LOCUS       DAOSIT010000001        19006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_3133_length_19006_cov_6.504564, whole genome shotgun sequence.
ACCESSION   DAOSIT010000001 DAOSIT010000000
VERSION     DAOSIT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19006
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_19006_cov_6.504564"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="cysW"
                     /locus_tag="PLL14_00005"
     CDS             complement(<1..467)
                     /gene="cysW"
                     /locus_tag="PLL14_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806780.1"
                     /note="Part of the ABC transporter complex cysAWTP
                     involved in sulfate/thiosulfate import; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HOG02327.1"
                     /translation="MRGNKLTRATQEPAWVRYTLLTVGLGFLFFFLGLPLVAVFVEAL
                     AQGLPIYLEALKESETRSAIWLTIVIALAVLPFNILFGVAAAWAIAKFEFKGKSLLIT
                     LIDLPFAVSPVVAGLIFILMFGSQGYFGEWLAAKDVKIIFAVPGMILATLFIT"
     gene            complement(449..1339)
                     /gene="cysT"
                     /locus_tag="PLL14_00010"
     CDS             complement(449..1339)
                     /gene="cysT"
                     /locus_tag="PLL14_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOG02328.1"
                     /translation="MSAKSQRVLPGFGLALGYTLLYLSLLILLPLGGMILKASELSFG
                     QILDIALGERSLAAFRVTFLSSLLAASINAFFGLIVAWILVRYRFPGKRFVDALVDLP
                     FALPTAVAGITLATLYAGNGWLGQYLEPLGVKVAYTPLGIIVALTFITLPFVVRTLQP
                     VIEDIETEVEEAAASLGASRWQTFVKAIFPAIGPALLTGFALAFARALGEFGSVIFIA
                     GNLPLISEIVPLLIISKLEQYDVLGATALAVVMLAFSFVLLLVINALQWWAANRHKNS
                     EPLEVAAGKAKAGAACVATN"
     gene            complement(1429..2427)
                     /locus_tag="PLL14_00015"
     CDS             complement(1429..2427)
                     /locus_tag="PLL14_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG02329.1"
                     /translation="MTHFRKSIATVLLSLVAGAALADATLLNASYDVARDVYKDYNPM
                     FQKYWKANAGESVELKQSHGGSTKQVRAVADGLEADVVTMNQANDIQFLAEKALVAKD
                     WAKKFPNNASPYTSAMVFIVRKGNPKALKDWSDLAAPGIQVILPHPKNTGNGRNTYLS
                     AWAWALKQPGGSDAKAQEFVGKLLRNAPLFAAGGRDATTTFMQRGLGDVLVTFESEAE
                     MIAKEFGKGNFEVVYPSLTMQTEFPVAVVEKVVDKKGTRKQATAYLEYLWSKEGQENA
                     AQNYLRPRDAELLKKYGAFFPPVKTFTVDEVFGGANKAFATHFKDGGTFDQIYVAK"
     gene            complement(2654..4969)
                     /locus_tag="PLL14_00020"
     CDS             complement(2654..4969)
                     /locus_tag="PLL14_00020"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG02330.1"
                     /translation="MSHDHAIHGHTHHATSARDPVCGMDVSLDAGKPTAEHAGTVFHF
                     CSQKCHDKFVADPQRYLSPAPTKAEAESAGTEYVCPMHPEVRQFGPGTCPKCGMALEP
                     AVPELEREEDDGEFRDFRRRFWGSLPLTVAVVLLAMAGHLVAGLTPAARTWAELLLST
                     PVVLWAGGPFFVRGWQSIVRRSPNMWTLIGIGTGAAFVYSVVAALAPGLFPDSFEAHG
                     RIGVYFEAAAVIISLTLLGQMLELRARSKTSAAIKSLLGLAPKTARRIRPDGSEEDVP
                     LTHVHVGDILRVRPGEKVPVDGEVTEGASAVDESMLTGEPLPVTKWPGDKVIGATLNT
                     SGSLVIRSEKVGAQTVLAQIVQMVAQAQRSKAPMQRLADVVAGYFVVAVVGIALLSFF
                     GWGIFGGERGWLFGLINAVSVLIIACPCALGLATPMSIMVATGKAATQGILFRDAAAI
                     ERLCEIDTLIVDKTGTLTEGRPAFERAVAAPGLAEDEVLRLAASLDQGSEHPLADAIV
                     RAAREKGLTLDKAENFESSSGIGVRGSVGGRRLALGNTVLMEQEGADVSPLAAQAEEL
                     RREGASVMHLAAGSRLLGLLAVADPVKTSTPEALAVLRQAGLRVIMATGDGETTARAV
                     GQRLGIDEVYGEVKPADKLALVERLQKEGHVVAMAGDGINDAPALARADVGIAMGTGT
                     DVAINSAQVTLVKGDLRGIARARLLSAATVGNMRQNLAFAFLYNALGVPIAAGVLYPS
                     FGLVLSPIIAAAAMSLSSVSVVGNALRLRGS"
     gene            complement(4974..5636)
                     /locus_tag="PLL14_00025"
     CDS             complement(4974..5636)
                     /locus_tag="PLL14_00025"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOG02331.1"
                     /translation="MTETAPAYGLWSLVLINSVLFIFFAFSFFKPQSARDWRTLGMFS
                     AFVLALFTEMYGFPLTIYLLSGWLTKQFPGVDFLAHDSGHLLEVMFGWRANPHFGPFH
                     LLSAALIGGGFWSLAAAWRVLYAAQASGRLATSGPYARVRHPQYVAFVLIMVGFLLQW
                     PTLITLVMFPILLVVYVRLARREERDSAAAFGEAWIRYASMTPAFFPRWNDGQNPASP
                     RS"
     gene            complement(5636..5896)
                     /locus_tag="PLL14_00030"
     CDS             complement(5636..5896)
                     /locus_tag="PLL14_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HOG02332.1"
                     /translation="MQTHDHSPHGTLHATTARISRRSRWVLGGFALLAVLLLAFEHWM
                     HLSGLIGWLPYLVLLACPLMHLFMHGGHGHGGGDGRSNDGAG"
     gene            complement(5883..6038)
                     /locus_tag="PLL14_00035"
     CDS             complement(5883..6038)
                     /locus_tag="PLL14_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02333.1"
                     /translation="MGIGMILLWLIPIALLVWLFAKAAAGRTSKDETHRPPPPKSLPA
                     GEYDANP"
     gene            complement(6091..6474)
                     /locus_tag="PLL14_00040"
     CDS             complement(6091..6474)
                     /locus_tag="PLL14_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02334.1"
                     /translation="MPAFAEESHHPAQPGGAPTTQAQADGKTVQKMQDNLGRMHTQLQ
                     RLAKAKTADKRQKLMDEHMKTMQENMGMATGMQSGMMDCPMMQGGMGMMGQGKVSQHD
                     MMSKRMEMMMQGRMGMPLGEPPKPA"
     gene            complement(6648..7085)
                     /locus_tag="PLL14_00045"
     CDS             complement(6648..7085)
                     /locus_tag="PLL14_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOG02335.1"
                     /translation="MKPAIRTTVLLALFAPAVWGAEALPTVKVYKTPTCGCCSNWVDH
                     MKASGFKVVTQDMNDVTPHKQRLGVPPAMGSCHTAEVAGYLIEGHVPAADVKRLLAEK
                     PQAKGLVSPGMPQSAPGMDAPGKQPYEIFLVRQDGSTATYARH"
     gene            complement(7212..7739)
                     /locus_tag="PLL14_00050"
     CDS             complement(7212..7739)
                     /locus_tag="PLL14_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02336.1"
                     /translation="MDIVAHGLWAGLGIAWAGKYHRINTKTKMATIGLAVAPDLIHLL
                     PLMIAAPFAANGLEIFTAYVNASPGFEPPLSPNLNQLTHHLHCVMHSALVAGAITAAV
                     WLWLGRLWVPLLGWWSHILIDVFTHSAEFYPSPVLYPLTYWGFDGIAWNAPWFMSINY
                     AVMTIVAYIIFLRRN"
     gene            complement(7776..8243)
                     /locus_tag="PLL14_00055"
     CDS             complement(7776..8243)
                     /locus_tag="PLL14_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02337.1"
                     /translation="MKKALFLIAAASAFALASPTLLAHTDEALDTQKAPNGGQLRMAG
                     AYHYELVVAKDSKEAKDNPVVVYVTDHAGTKVPTAGAKGTVTILSGKAKVTVSLIPDG
                     DNRLKGSGAYASAPDMKAIVSVTLPNKSPEQARFTPLDKPMLSTQDGHAGHSH"
     gene            complement(8275..11379)
                     /locus_tag="PLL14_00060"
     CDS             complement(8275..11379)
                     /locus_tag="PLL14_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG02338.1"
                     /translation="MFDYIVHTALKQRLIVLGISLLLIIYGAITLRQMPVDVFPDLNK
                     PTVTLMTEAGGMAPEEVEQLVTFPVESSMNGMPGVTRVRSVSGIGLSIIYVEFEWGSD
                     IYRNRQQIAERLNLVREALPPGIVPQLGPISSIMGEILLIALPADPDKISPMTVREYA
                     DWVMRPRLLTIPGVAQVIPIGGEVRQYRVEIKPAQLQALGIEREKLEAALKDFGANTS
                     GGFLEAQGREYLIRQIGRSSRIENLQNLVVATKNGQPILLRQVADVKLAPAVKRGDGS
                     YKGKPAVILSVQKQPAADSVQLTREVEKAMAELGKSLPKGFDTPSFLFKQADFIEHSV
                     NNVEEALRDGAILVAVILFVFLLNVRTTLISLMAIPVSLLVTALVFHYFGLSINTMTL
                     GGLAIAIGELVDDAVVGVENVLRRLKLNRATDSPRPVAEVIAAATLEVRSAIVYATVI
                     IVLVFVPLFVLPGIEGRLFTPLGVAYIVSILASMIVSVTLTPVMAYYLLPQMKQLHAG
                     DSPLVIWLKRWDAKLLHWSFGHARRLLAGAAALVIIVAASVPFFPRSFLPPFNEGTLT
                     VNVLLNPGTSLAESNSIGTLAEQLVMQVPEVTQLGRRTGRAELDEHAEGVHYTEMDVD
                     LKASARSREEIIADIRQRLASLPAASNVGQPISHRLDHLLSGVRAQIALKIYGDDLDT
                     LRGLAAEMRDRLARIPGVADLQIEKQVLIPQIKIRLDYEQAARYGVAPGSLLRGLEQM
                     IEGEKITQIIEGNRRFDLLVRLPESARGPQALADLLIETPTGYVPLSKIASVEESDGP
                     NQVSRENSRRRIVLSANTDGRDMSKVIADIRAELAAKPLPEGYFTALEGQFQAQEQAA
                     KLITLLALVSLTMIFMVLYSRYRSMALTLIIMGNIPLALIGSVIALWISGQPLSVAAL
                     VGFITLTGIATRNGILKISHYINLCAFEGETFGQHMIVRGSLERLTPVLMTALVAAFA
                     LMPLLLSADAPGKEVLHPVAVVIFGGLISSTLLDTLLTPVMFWLWGQRPLDSLLASQE
                     KESF"
     gene            complement(11381..12463)
                     /locus_tag="PLL14_00065"
     CDS             complement(11381..12463)
                     /locus_tag="PLL14_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG02339.1"
                     /translation="MAHGNEDHSQDAKKKAPAAVSAVGTTGVTAALQRLADGSLFVPK
                     PVQRQLGLRTQPVRIAELAATVELNGQIIPDPETSGRVQAMFAGSVIPGPKGMPTAGR
                     KVAKGEVLVYLRPISGAIERGNQKASLAELEAQLAIAKGKMKRFEQLEGAVPQKEIEA
                     ARIEHAALQQRRAYVRASIDSAEPLVAPAAGIISASHHLLAGQVVDAKEVLFEIVDPA
                     RLAVEALAYDVGIAKTLVSASALADQTALDLKFVGGGQQLREQALPLLFRITNRNAAV
                     AVGQPVKVFARTEQGVKGATAPRTALTQVGAGETAVWVHIEAERFVARRIRHQSLDAA
                     NVAILDGLHDGDRVVTVGAGLLSQVR"
     gene            complement(12532..13053)
                     /locus_tag="PLL14_00070"
     CDS             complement(12532..13053)
                     /locus_tag="PLL14_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02340.1"
                     /translation="MRHAVAVVLFSVAFSAFGHGGEDHGAPPPPVTQAVEPRAAAATE
                     EFEVVTSLEGKKLVVYVDRFASNEPVVQAKVEIEGAGLKGVASETTPGTYVLDVATPL
                     PPAMHPLTISVEAGDSIDLLCATLDTTASVTTEVHTHGWREWVVWALPGALLLITGIW
                     FAVRRHKRAKKGI"
     gene            complement(13050..14309)
                     /locus_tag="PLL14_00075"
     CDS             complement(13050..14309)
                     /locus_tag="PLL14_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG02341.1"
                     /translation="MKPVRFHQFRQLRRAAVCATVLLAGLSVQAAETPKSGLATAVEQ
                     AWHLNPQAAGLDAREAEARAAQDVAAGLTPEPGSVSIGNLNDRFDRNQGKQEYEVELA
                     VPLWLPGQKAAREAEASSRIDEAVARRTAVRLEVSGEVREAWWALAAARSAKVLASRR
                     LETARALETDVMRRYKVGELSRIDANLAQSETHAANAELIEAEANLLQAEQAVQVLTG
                     SPAPKNLDEESPTTRRLADETGDSAASHPLLAVTAAAARSARARVKVADESRRAAPEL
                     TLRVVRERDDFADSYSNNVGIKLKIPFSSGSQVRRETSAAQAEANQSDADLLRTHTRV
                     RLDAERAQRNLQSAERQFTMAQERRQLSAENLRLAEKAFALGESDLTTLLRIRSAAFD
                     ADAFLDRQRVARAAAISRLNQSMGVLP"
     gene            14529..15206
                     /locus_tag="PLL14_00080"
     CDS             14529..15206
                     /locus_tag="PLL14_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HOG02342.1"
                     /translation="MKILVVEDEIKTGDYLRQGLTEAGFVVDLARDGLDGLHLAQTDA
                     YDLAILDVMLPGIDGWAVLQGIRRAGKDMPVLFLTARDQVQDRVHGLELGADDYMVKP
                     FAFAELLARVRIHLRRGSKTKESEYLRAADLELDLMRRRVTRAGRRIDLTVKEFALLE
                     LLLRRQGEVLPRSLIASQVWDMNFDSDTNVIEVAIKRLRLKIDEDFELKLIRTVRGMG
                     YVLEIAE"
     gene            15203..16591
                     /locus_tag="PLL14_00085"
     CDS             15203..16591
                     /locus_tag="PLL14_00085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HOG02343.1"
                     /translation="MKSAGRKSIIFRLTLLFASASTAVLLVLGLLIGNSVEHHFEVQD
                     MEVLTGKLELTRHALEKVHSQSDLDALPQQLDDSLVGHHGLAVIVVAPSGQTLFVTSG
                     AEFPQVLLARSPDMTNSGPIIWKSTDHIPLRGISAMVKTGIEGAKPAVVAVATDISKH
                     EHFMSWFRFTLWSVVILAALMTGFLGWVAVRRGLAPLHAIKLEAAAITADRLHSRLAT
                     ESIPIELVDLAETLNQMLARLEDSFRRLSDFSSDIAHELRTPVSNLLTQTQVMLLRVR
                     TIDEYQDVLASNAEELERMAKMIADMLFLAKADNNLVVPNLEAVDLRAEAEGLASFYE
                     AVAEENSVALTVDGSATVTGDRLMLRRAIGNLLSNAFGHTPRGGYVRIQIGKNEDRMA
                     TIQVENSGETISPEHLPRLFDRFYRADPSRKRLADGVGLGLALTRSIIQAHGGSVSVR
                     SADGVTIFELQM"
     gene            complement(16848..17147)
                     /locus_tag="PLL14_00090"
     CDS             complement(16848..17147)
                     /locus_tag="PLL14_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopK family periplasmic copper-binding protein"
                     /protein_id="HOG02344.1"
                     /translation="MKTKLAKLALIAVLSTSGATTAFADDAARAEAKQVVDLKNGATL
                     YIFNDGKMAIEDRYGRATRTTPGTEVETSDGRKITTQSDEVARLNSLINKGHKGG"
     gene            17745..18152
                     /locus_tag="PLL14_00095"
     CDS             17745..18152
                     /locus_tag="PLL14_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02345.1"
                     /translation="MANGLKQRFPFVEAIVRGTSTLDLAWTGAGEPTAGEMEARCDLP
                     RVGDLAELGENTGNHRQRLQRFAELVGREILSPVLAYGVDLACLERGNCGEHAACASI
                     DAVVKAGMCRAMEETGHGSHHLKCQLWLLGKAM"
     gene            18234..18545
                     /locus_tag="PLL14_00100"
     CDS             18234..18545
                     /locus_tag="PLL14_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOG02346.1"
                     /translation="MIRSDLITVLASRFPSLVAKDAEIAVKEILDAIGIALAQGDRVE
                     IRGFGSFRLNYRPARTGRNPKSGEPVSVAEKYVPHFQAGKEMRERVEQSVKPAPLQRA
                     A"
     gene            complement(18693..>19006)
                     /locus_tag="PLL14_00105"
     CDS             complement(18693..>19006)
                     /locus_tag="PLL14_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="muramidase"
                     /protein_id="HOG02347.1"
                     /translation="RLRQHIKKDRGDLWTRAANYHSRTPQYNAVYRADLLVKAGRWAD
                     WLEARFVTVDVTKVGALPSSMPVALATVQPSSAQTTKAATVAWNTSGYVPRKVIIGGR
                     P"
BASE COUNT         3666 a         5783 c         5923 g         3634 t
ORIGIN      
        1 aaggtgatga acagggtggc gaggatcatg cccggcacgg cgaagatgat cttgacgtcc
       61 ttggccgcca gccactcgcc gaaatagccc tgcgagccga acatgaggat gaagatcagg
      121 ccggcgacca ccggcgacac cgcgaagggc aggtcgatca gcgtgatcag caggctcttg
      181 cccttgaact cgaacttggc gatggcccag gccgcagcga cgccgaacag gatgttgaag
      241 ggcagcacgg ccagcgcaat gacgatggtc agccagatcg ccgaacgggt ttccgattcc
      301 ttcagcgcct caagatagat cggcaggccc tgcgccagcg cttcgacgaa aaccgcgacc
      361 agcggcagcc cgaggaagaa gaacaggaag ccgagaccaa ccgtcagcag cgtgtaacgt
      421 acccaggccg gttcctgcgt ggcgcgcgtc agtttgttgc cacgcatgcc gcacctgcct
      481 tggccttgcc agccgccact tccaacggct cgctgttctt gtgccgattg gctgcccacc
      541 attgcagcgc attgatgacc agcagcagca cgaaggaaaa ggccagcatg accacggcca
      601 gcgcggtggc gccgagcacg tcgtactgct ccagcttgga gatgatcagc agcggcacga
      661 tttcggaaat cagcggcagg ttgccggcga tgaagatgac cgagccgaat tcgccgagcg
      721 cccgggcaaa ggccagcgcg aagccggtga gcaaggccgg gccgatggcc gggaaaatcg
      781 cttttacgaa ggtctgccag cgcgaggcgc cgagcgaggc ggctgcctcc tcgacttcgg
      841 tttcgatatc ctcgatcacc ggttgcaagg tgcgcacgac gaagggcagg gtaatgaagg
      901 tcagggcgac gatgatgccg agcggcgtat aggccacctt gacccccagt ggctcaaggt
      961 actgccccaa ccagccgttg ccggcgtaaa gcgtggccag cgtgatgccg gccaccgctg
     1021 tcggcagtgc gaagggcaga tcgaccagcg catcgacgaa gcgcttgccg gggaagcgat
     1081 agcggacgag gatccaggcg acgataagcc cgaagaaggc attgatcgac gcggccagca
     1141 gcgacgacag gaaggtgacg cgaaaggctg ccagcgaccg ttcgccgagc gcaatgtcga
     1201 gaatctggcc gaaactgagt tcggatgcct tgaggatcat gccgcccagg ggcagcagga
     1261 tcagcagcga gagatagagc agcgtgtagc ccagcgccag accgaagcca ggcaacacgc
     1321 gttgggattt tgcggacatg agtattcttg tttctagcca aattttccgg tcgaccgcag
     1381 caaagcctca ggccgggcag aacccggccg gctttggcag gcgaacgctt acttggcgac
     1441 gtaaatctgg tcgaaagtgc cgccatcctt gaagtgggtg gcaaatgcct tgttggcacc
     1501 gccgaacacc tcatcgacgg tgaatgtctt caccggcggg aagaacgccc catacttctt
     1561 cagcagctcg gcgtcacgcg gccgcaggta gttctgcgcg gcgttctcct ggccttcctt
     1621 ggaccacagg tactccaggt aagccgtggc ctgcttgcgc gttcccttct tgtccaccac
     1681 cttctcgacg acagcgaccg ggaactcggt ctgcatggtc aggctgggat agaccacctc
     1741 gaaattgccc ttgccgaact ccttggcgat catttcggct tcggattcga aagtcaccag
     1801 tacgtcgccc agaccgcgct gcatgaaggt cgtcgtcgca tcacggcctc cggcggcgaa
     1861 caacggggcg ttcctcagca gcttgccgac gaattcctgc gccttggcat cgctgccacc
     1921 cggctgcttc aaggcccagg cccaagccga caggtaggta ttgcggccat tgccggtgtt
     1981 cttcgggtgc ggcaggatga cttgaatgcc gggcgcagcc agatcggacc aatccttcag
     2041 ggctttcggg ttgcccttgc gcacgatgaa gaccatcgcc gacgtgtacg gcgacgcgtt
     2101 gttcgggaat ttcttcgccc agtccttggc gaccagagct ttctcggcca agaactggat
     2161 atcgttggcc tgattcatcg tcaccacgtc ggcctccagc ccgtcggcca cggcccgcac
     2221 ctgcttggtc gagccgccgt gcgactgctt cagctccacg ctttcgccag cgttggcttt
     2281 ccagtacttc tggaacatcg ggttgtagtc cttgtagaca tcgcgcgcga cgtcgtagga
     2341 cgcgttgagc agcgtggcat cggccagggc agccccggca accaggctga gcagaacggt
     2401 agctatcgac ttacggaaat gggtcatgat tcacctcgct atggatggcc gctaatctag
     2461 cggggtcact tataaccaca aagacagttt ttggatttac ttattacacc aagcgcaaag
     2521 ccgcacataa accaatcatc ggcactattc gccattcatg cgagcggcca tcattgaaaa
     2581 ttcttcaagc cgccgacaat caagatgggg catattgagt gatggcgtgt gtcaggcaag
     2641 gctgtcccga tcttcagcta ccccgcagcc gcaatgcgtt gccgaccacg ctcaccgacg
     2701 acaggctcat ggcagcagcg gcgatgatcg gcgacagaac caatccaaag ctcgggtaca
     2761 gcacacccgc cgcgatcggc acgcccagtg cgttgtagag gaaggcaaag gcgaggtttt
     2821 ggcgcatgtt gccgaccgtt gccgccgaga gcaggcgagc gcgggcgatg ccgcgcaggt
     2881 cgcccttgac cagcgtgact tgggcactgt taatggctac atcagtgccg gtgcccatgg
     2941 cgataccgac gtccgccctg gcaagtgccg gggcgtcgtt gatgccgtcg ccggccatgg
     3001 ccacgacatg cccttccttc tgcaggcgct cgaccagtgc cagtttgtcg gccggtttga
     3061 cctcgccgta cacctcgtcg atgccgagcc gctgtccgac ggcgcgcgcc gtcgtctcgc
     3121 catcgccggt ggccatgatc actcgcagtc ctgcttggcg caacacggcc agagcctcag
     3181 gcgtgcttgt tttgaccgga tcggcgacag ccagcaggcc cagtagccga ctgccggcgg
     3241 ccaggtgcat gacgctcgcc ccctcgcgcc gcagttcctc ggcctgagcc gccaagggcg
     3301 acacgtccgc gccttcctgt tccatcagca cggtattgcc cagcgcaagg cggcgtccgc
     3361 cgacgctgcc gcgcacgccg atgccgctgg aggactcgaa gttctcggcc ttgtccagcg
     3421 tcagcccctt ttcccgggcc gcccggacga tggcgtcggc gagcggatgc tcgctgccct
     3481 gatcgagact ggccgccagc cgcagtacct cgtcttcggc gagccccggc gccgcgaccg
     3541 cgcgctcgaa ggccgggcgg ccttccgtca gcgtgccggt cttgtccacg atcagggtgt
     3601 cgatctcgca caggcgctcg atggccgccg catcgcggaa caatatcccc tgcgtcgcgg
     3661 ccttgccggt ggcgaccatg atcgacatag gcgtggccag gcccagcgcg caggggcagg
     3721 cgatgatcag gactgagacg gcgttgatga ggccgaacag ccagccgcgc tcgccgccga
     3781 agatccccca gccgaagaag gagagcagtg cgatcccgac caccgccacc acgaaatagc
     3841 cggccacgac gtcggccaga cgctgcatcg gcgctttgga acgctgtgcc tgggcgacca
     3901 tctggacgat ctgggcgagc acggtctgag caccgacctt ttcggagcgg atcaccaggc
     3961 tgcctgaggt gttgagcgta gcgccgatca ccttgtcgcc gggccacttt gtcacgggca
     4021 ggggttcgcc ggtcagcatg gattcgtcaa ccgcactggc gccttccgtg acttcgccat
     4081 ccaccggcac cttctcgccc ggccgcacgc gcaggatgtc accgacgtga acatgcgtca
     4141 gcggcacgtc ttcctcgctg ccgtccggcc ggatgcgtcg ggcggtcttg ggtgcgaggc
     4201 cgagcaggga cttgatcgcc gccgaggttt tcgagcgggc gcgcagttcc agcatctgcc
     4261 cgagcagggt cagcgagatg atcactgctg ccgcctcgaa atagacgccg atgcggccgt
     4321 gggcttcgaa cgagtccggg aacaggccgg gcgccaaggc cgccacgaca ctgtagacaa
     4381 aagcggcgcc ggtgccgatg ccgatcagtg tccacatgtt cgggctgcgc cggacgatgg
     4441 actgccagcc gcgcacgaag aacggccctc ccgcccaaag aacgaccgga gtgctgagta
     4501 acaactcggc ccaggtacgc gctgccgggg tcaatccggc gacgaggtgc ccggccatgg
     4561 ccagcaggac tacggcgacg gtgagcggca gactgcccca gaagcgtcgc cggaaatccc
     4621 gaaactcacc atcgtcttcc tcacgttcca gttcgggcac ggcgggttcc agggccatgc
     4681 cgcacttcgg gcaggtgccc ggcccgaact ggcggacttc cgggtgcatt gggcagacat
     4741 attcggtgcc cgccgactct gcctctgctt tcgtcggcgc gggagacaga tagcgctgcg
     4801 ggtcggcaac gaacttgtcg tggcatttct gcgagcagaa gtgaaaaacc gtgcccgcgt
     4861 gttctgcagt cggcttaccg gcatccagcg acacatccat gccgcatacc ggatctctcg
     4921 cgctcgtggc atggtgggtg tggccatgaa tagcgtgatc gtgactcatg cggtcaactc
     4981 cttggcgacg ccgggttctg tccgtcgttc caacgtggaa agaaggccgg cgtcatgctg
     5041 gcatagcgta tccatgcctc gccaaaggca gcagcggaat cgcgctcctc gcgccgggcg
     5101 agacgcacat acacgacgag caggatgggg aacatcacca gcgtaatcag cgttggccat
     5161 tgaagcagga acccgaccat gatcaggacg aaggcgacgt actgcggatg acgcacacgc
     5221 gcgtaagggc cgctggtcgc cagccgaccg cttgcctgcg ccgcatagag cacgcgccat
     5281 gcggccgcga gcgaccagaa gccgccgccg atcaacgccg ctgacaacag gtggaacggc
     5341 ccgaagtgcg gattggcgcg ccagccgaac atcacttcca gaagatgccc cgagtcatgg
     5401 gcgaggaagt ccacgccggg gaactgcttg gtcagccagc ccgagagcag atagatggtg
     5461 agcggaaagc catacatctc cgtgaacaac gcaagcacga aggccgaaaa catgcccaga
     5521 gtgcgccagt cgcgagcgct ttgaggtttg aagaagctga aggcaaagaa gatgaacagc
     5581 accgagttga tcagcaccag cgaccacaaa ccataggcgg gagcagtttc tgtcattatc
     5641 ctgctccatc gttggatctg ccgtcgcctc caccatggcc atggccgccg tgcatgaaca
     5701 ggtgcatcag cgggcaggct agcaggacca ggtagggcag ccagccaatc aggccggaga
     5761 ggtgcatcca gtgctcaaaa gccaggagca gaacggcgag gagtgcgaag cctcccaaga
     5821 cccagcggga gcgccggctt atcctggcgg ttgtcgcatg cagtgtcccg tgtggggaat
     5881 ggtcatgggt ttgcatcgta ttcacctgct ggtaatgact taggtggcgg gggtcgatgc
     5941 gtttcgtcct tgctggtgcg accggccgcc gctttggcga acagccagac cagcaaggcg
     6001 atcggtatca accagagcag aatcatgccg atgcccatca ggcctcctcc gtgatcgtat
     6061 ccccacatgg caatgcctcc atgaatgtgg tcaggctggt ttcgggggct cgcccagcgg
     6121 catgcccatg cggccctgca tcatcatctc catgcgtttg gacatcatgt cgtgctggga
     6181 aaccttgcct tgtcccatca ttcccatgcc cccctgcatc atcggacagt ccatcatgcc
     6241 cgattgcatc cccgtggcca tgcccatgtt ctcctgcatc gtcttcatat gctcgtccat
     6301 gagtttctgg cgtttgtcgg cggtcttcgc tttcgccagc cgctggagct gggtgtgcat
     6361 tcggcccagg ttgtcctgca tcttttgcac ggtcttgcca tccgcctgcg cctgggtagt
     6421 tggcgccccg ccgggttgcg ccgggtgatg cgattcctca gcgaatgccg gcatggcaag
     6481 ggcaagtgaa acagcaattg ctgaaagggc gacggttctc aaaaaattct ctttgatcga
     6541 tgttgtgaaa cgacttcaaa gccagaagtg caggcccgcc acccagcgcg cttcgccgga
     6601 aggacgagag aaaatccgct gtgtcgcatc aattaatcct cccggggtta atgacgtgcg
     6661 taagttgcgg tactgccgtc ctggcgcacg aggaaaatct cgtagggctg cttgccgggc
     6721 gcgtccatgc cgggtgcgct ctgcggcatt cccggggaga cgaggccctt ggcctgcggc
     6781 ttctcggcga gcaggcgctt gacgtcggcg gccggcacgt ggccctcgat caggtagccg
     6841 gcaacctcgg cggtgtggca ggagcccatc gccggtggga cgcccagtcg ctgcttgtgc
     6901 ggggtgacgt cgttcatgtc ctgcgtgacg accttgaacc cgctggcctt catgtgatcc
     6961 acccaattgc tgcagcagcc gcaggtcggt gtcttgtaaa ccttgacggt cggcagcgct
     7021 tcggcccccc atacggcagg cgcgaacagg gcaagcagga ctgtggtgcg gatagcgggt
     7081 ttcatgtggg actcctttgt gattcgatgg gttcagtatg aactgcggac accaacacca
     7141 gcctgaccgc gggattacat ttctgtcatt ctgcggcgca agtaggcatc atttgaaatc
     7201 ggtggtatcg atcaatttcg tcgcaagaag atgatgtagg caacgatggt catcacagcg
     7261 tagttgatag acataaacca cggcgcattc catgcgatgc catcgaatcc ccagtaggtc
     7321 agtggatata agacggggga agggtagaac tccgccgaat gcgtaaatac atctatcaga
     7381 atatgcgacc accaacccaa tagtggaacc cagaggcgcc ccagccacaa ccaaacggca
     7441 gcagtaatcg cgccggccac caaggcactg tgcatcacgc aatgcagatg atgggtaagt
     7501 tgattgaggt tgggggacaa cggaggctca aagcccggtg aagcattgac ataggcagtg
     7561 aatatttcca gcccatttgc cgcgaatggc gccgcgatca tcagggggag caaatgtatg
     7621 agatcggggg caaccgccag cccgatcgtc gccatcttcg tctttgtgtt tatgcgatga
     7681 tattttccgg cccaagcaat gccgaggcca gcccaaagac catgtgcaac gatgtccaag
     7741 ttttcgctcc cgaagtgttc aacggcgcgt tccgttcagt gcgagtgccc ggcgtgcccg
     7801 tcctgagtag agagcatcgg tttgtccagt ggcgtgaagc gagcttgttc tggagacttg
     7861 ttcgggagcg tgaccgaaac gatagccttc atatccggcg ctgaggcgta ggcgcccgaa
     7921 ccctttagtc ggttgtcgcc atccggtatc aggctgacgg tgaccttggc ttttccggaa
     7981 aggatggtta ccgtaccttt tgcacctgcc gtcggcacct ttgtaccggc atggtcggtg
     8041 acatacacga ccaccgggtt gtccttcgct tccttgctgt ccttggcgac taccagttcg
     8101 tagtgatagg cgccggccat gcgcaactgg ccgccgttgg gcgctttctg ggtatccagg
     8161 gcttcgtcgg tgtgggcaag caacgtcggg ctcgccagag caaatgcact ggcggcagca
     8221 atcaggaaca gggctttctt catggcaaac atctccttgg gcgggttgaa agggtcagaa
     8281 actctctttt tcctgactgg ccaacaggct atccagggga cgttgtcccc atagccagaa
     8341 catcaccggg gtcagcagcg tatcgagcaa agtggagctg ataaggccac cgaagatcac
     8401 taccgccacc ggatgcagca cttctttgcc cggagcatcg gccgagagca gcagcggcat
     8461 cagcgcgaag gcggcgacca gcgcggtcat cagcaccggg gtgagccgct ccagcgaccc
     8521 gcgcacgatc atgtgctgac cgaaggtttc gccttcgaag gcgcacagat tgatgtaatg
     8581 actgatcttg aggatgccat tgcgcgtggc gatgccggtt aaggtgatga agccgaccag
     8641 cgcagcgacc gacagcggct ggccggaaat ccacaaggcg atcacgctgc cgatcaacgc
     8701 cagcgggatg ttgcccatga tgatcagtgt cagcgccatc gagcggtagc ggctgtagag
     8761 caccatgaag atcatcgtca gcgacaccag cgccagcaag gtgatgagtt tggcagcctg
     8821 ctcctgcgcc tggaactgtc cttcgagcgc ggtgaaatag ccctccggga gcggcttcgc
     8881 ggccagctcg gcgcggatgt cggcgatcac ctttgacatg tcgcggccgt cggtgttggc
     8941 cgagagcacg atacggcgac gtgagttttc gcggctgacc tggttgggac catcgctttc
     9001 ctcgacgctg gcaatcttgg acagtgggac gtagcccgtt ggcgtctcga tcaacaggtc
     9061 ggccagcgcc tgcggcccgc gcgcggattc cggcaggcgc accaatagat cgaagcggcg
     9121 gttgccttcg atgatctggg tgattttctc gccctcgatc atctgttcca gcccacgcaa
     9181 caaactgccg ggcgccacgc cgtagcgagc tgcctgctcg tagtcgaggc gaatcttgat
     9241 ctgcgggatc agcacctgct tttcgatctg cagatcggcg acgccgggaa tccgtgccag
     9301 ccggtcgcgc atctcggccg ccaggccgcg cagggtatcg agatcgtcgc cgtagatctt
     9361 cagcgcaatc tgggcgcgca caccggaaag gaggtgatca agccggtgcg aaattggctg
     9421 gccgacgttg gaggcggccg gcaggctggc aagacgctgc cggatgtcgg caatgatctc
     9481 ttcacggctg cgcgccgagg ctttcaggtc gacgtccatt tcggtgtagt gcacgccttc
     9541 cgcatgctca tcgagctccg cgcggccggt gcggcgtccg agctgcgtga cctcgggcac
     9601 ttgcatcacc agttgctcgg ccaaagtgcc gatgctgttg gattcggcga gcgaggtacc
     9661 gggattgagc aggacattga ccgtcagcgt gccctcgttg aaaggcggca ggaaggagcg
     9721 cggaaagaag ggcacgctcg ccgcgacgat gatgaccagc gccgcggccc cggccagcag
     9781 gcggcgtgca tgaccgaacg accaatgcag aagcttggca tcccagcgct tgagccagat
     9841 caccaacggg ctgtccccgg cgtggagctg cttcatctgc ggcagcaggt agtaggccat
     9901 caccggcgtc agcgttaccg aaacgatcat gctggcgaga atcgagacga tgtaggcgac
     9961 gccgagcggg gtaaacagcc ggccctcgat gcccggcagc acgaacagcg gcacgaacac
    10021 caggacgatg atcacggtcg catagacgat ggcggagcgc acttccagcg tcgcggcggc
    10081 gatgacttcc gccaccgggc gcggcgaatc agtggcccgg ttcagcttca gacgtcgcaa
    10141 cacgttctcc acgccgacca ccgcgtcatc caccagctcg ccgatggcga tggccaaacc
    10201 gcccagcgtc atggtgttga tcgacaggcc gaagtaatgg aacaccagcg ccgtaacgag
    10261 cagcgacacc ggaatcgcca tcaacgaaat cagcgtggtg cgcacattga gcaggaacac
    10321 gaagaggatc acggccacca ggatggcgcc gtcgcgtagt gcctcttcaa cgttgtttac
    10381 cgaatgctcg atgaagtcgg cctgcttgaa caggaaggac ggcgtatcga atcccttggg
    10441 caggctcttg ccgagctcgg ccattgcttt ctcgacttcg cgggtcagct ggacgctgtc
    10501 ggcagccggc tgtttctgca ccgacaaaat cactgccggc ttgcccttgt aactgccgtc
    10561 gccgcgcttg acggccgggg cgagtttcac gtccgccact tgccggagca ggatcggctg
    10621 gccattcttg gtcgccacaa cgagattttg caggttttca atgcgcgagc tgcggccgat
    10681 ctggcggatc aggtattcgc ggccctgggc ttcaagaaag ccgccgctgg tgttggcgcc
    10741 gaagtccttc agggcggcct ccagcttctc gcgctcgata cccagcgcct gcaactgtgc
    10801 cggcttgatc tccacccggt attgccgcac ctcgccaccg atcgggatga cctgggcgac
    10861 accgggaatc gtcagcagac gcggacgcat cacccagtct gcgtattcgc ggacggtcat
    10921 cggactgatc ttgtcgggat cggccgggag cgcgatcagc aaaatctcgc ccatgatcga
    10981 cgagatcggc ccgagctgcg gcacgatgcc gggcggcagg gcctcgcgca ccagattcag
    11041 ccgctcggca atctgctgac ggttgcgata aatgtccgaa ccccattcga attcgacata
    11101 gatgatcgac agaccgatgc ctgacaccga gcggacgcgc gtcacgcccg gcatgccgtt
    11161 catgctcgat tcgaccggga aggtcaccag ttgctcgacc tcctcagggg ccatgccgcc
    11221 ggcctcggtc atcagcgtca ccgttggctt gttgaggtcg gggaagacgt cgaccggcat
    11281 ctgccgcagg gtgatcgcac cgtagatgat cagcagcagt gagatgccga ggacgatcaa
    11341 ccgctgcttg agcgcggtgt gaacgatgta gtcgaacata tcagcgcacc tgcgagagca
    11401 gcccggcgcc gacggtcacc acgcggtcgc cgtcatgcag gccgtcgagg atggcgacgt
    11461 tggccgcatc gagcgactgg tggcgaatcc ggcgggcaac gaagcgctca gcctcgatat
    11521 gcacccagac cgccgtctcg ccagcaccga cttgtgtcag tgccgtcctc ggcgcagtcg
    11581 cacccttgac gccttgctcg gtgcgggcga acaccttgac cggctgaccg acggccactg
    11641 ccgcgttgcg gtttgtgatg cgaaacagca ggggcaaagc ctgctcgcgc agttgctgac
    11701 cgccgccgac aaacttgagg tccagcgccg tttgatctgc caaggcgctg gcggaaacca
    11761 gtgtcttggc gatgccgaca tcgtaagcca gcgcctccac ggcgaggcgg gcgggatcga
    11821 cgatttcaaa cagcacttcc ttggcgtcga ccacctgccc ggccagcaag tgatgcgagg
    11881 cgctgatgat gccggctgcc ggtgccacga ggggttcggc ggagtcgatg ctggcccgga
    11941 cataggcccg ccgttgctgc aaggcggcat gctcgatgcg cgccgcctcg atctccttct
    12001 gcggcacggc gccttcgagt tgctcgaagc gcttcatctt gcccttggcg atggccagtt
    12061 gcgcttcgag ttctgcgagg ctggctttct ggttgccacg ctcgatggca ccgctgatcg
    12121 gtcgcaggta gaccaggact tcgcccttgg cgacctttct tccggccgtc ggcatgccct
    12181 ttgggccagg gatgacgctg ccggcgaaca tggcctgcac ccgaccgctg gtttccggat
    12241 cgggaataat ctggccattc aattcgaccg tcgccgccag ttcggcgata cgcaccggct
    12301 gggtacgcaa gccgagctgg cgttgcaccg gcttcggcac gaacagactg ccgtcggcca
    12361 accgctgcag cgctgcagtc accccggttg tgcctaccgc cgacacagcg gccggcgcct
    12421 tcttcttggc gtcctgactg tgatcttcgt tcccgtgagc caaggctgcc tccgtgacca
    12481 tacttaatgc cagcgcggtc accagtacat gcaaatcgat cttgcgcatc atcaaatccc
    12541 cttcttggcg cgtttgtgac gccgaacggc aaaccatatt ccggtgatca gcagcagcgc
    12601 tccgggcaaa gcccagacga cccactcgcg ccagccgtgg gtatgcacct cagtggtcac
    12661 ggaggcggtg gtgtccagtg tggcgcagag gagatcgata ctgtcgccgg cctcgacgct
    12721 gatggtcagc gggtgcatgg ccggcggcag cggtgtggcg acatcgagca cgtaggttcc
    12781 gggtgtggtt tcgctggcca cgcccttcag ccccgcaccc tcgatttcca ccttggcctg
    12841 caccacgggt tcgttactgg cgaaacgatc cacatacacc accagcttct tgccttcgag
    12901 cgacgtgacg acttcgaatt cctctgtcgc tgcggcggca cggggctcga ccgcctgggt
    12961 aacagggggc ggcggtgcgc cgtgatcctc cccaccgtgg ccaaaagccg agaaggccac
    13021 ggaaaataga acaacggcaa cagcatgtct cacggcagca cccccatgga ttggttcaaa
    13081 cgcgatatcg cagcggcgcg ggcgacgcgc tggcggtcaa gaaaggcatc ggcatcgaag
    13141 gcggcggagc gaatgcgcag caaggtcgtc agatcggatt cgcctagcgc aaaggctttc
    13201 tcggcaaggc gcaggttctc ggcggaaagc tggcggcgct cctgcgccat ggtgaactgg
    13261 cgttcggccg attgcaggtt gcgctgcgcg cgctcggcgt cgagccggac ccgggtgtgg
    13321 gtacgcagca aatcggcatc ggactgattc gcctcggcct gtgccgccga cgtttcccga
    13381 cgtacttgcg aaccggacga gaacgggatc ttgagtttga tgcctacgtt gttggaatag
    13441 gagtcggcaa agtcgtcccg ctcgcgcacg acccgcagcg tcagttccgg cgcggcacga
    13501 cgcgactcat ccgcgacctt gacgcgcgca cgggcgctgc gcgcggcggc ggcggtaaca
    13561 gccagcaagg gatgagatgc ggctgaatcg ccggtttcat ccgccagtcg tcgcgttgtc
    13621 ggcgactctt cgtccagatt cttcggggca ggagaaccag taaggacttg tacggcctgc
    13681 tcggcctgga gcaggtttgc ttccgcttcg atcaattcgg cattggcggc atgggtctcg
    13741 gattgcgcga ggttggcatc gatgcgcgac agttcgccca ccttgtagcg ccgcatgacg
    13801 tcggtttcca aagcgcgggc ggtttccaga cgacgcgagg ccagaacctt ggcactgcgg
    13861 gctgcggcca acgcccacca tgcctcccgc acttcgccgc tgacctccag acgcacggcg
    13921 gtgcgtctgg caacggcttc gtcaatgcga ctgcttgcct cggcttcgcg cgccgctttc
    13981 tggccgggca gccacaacgg caccgccagt tcgacctcgt attcctgctt gccctgattg
    14041 cggtcgaacc ggtcgttcaa gttgccgatg ctgaccgaac ctggctcggg cgtcagccca
    14101 gccgcgacat cctgcgctgc gcgtgcttcg gcctcgcggg catccaatcc ggcggcttgc
    14161 gggttcaagt gccaggcctg ttcgacggct gtcgccagtc cggattttgg cgtttctgcc
    14221 gcctgtacgg atagaccggc aagcagtacg gtggcgcaaa ctgccgctcg cctcagttgg
    14281 cggaattgat gaaacctcac gggtttcatg actgccggga agaaagaagc aggaataggc
    14341 gacggcccgc aatttcctga cgatttcgag tatgagtttc cacagcgttc gctcctgttc
    14401 gacgatgagc gaattctgcg taacaccagt taacgccatg ctgaccttca gattacattt
    14461 cggtaatctt gggggattgc ctaagttgcc ggcgcagaat agcccaaatt gcaacacggg
    14521 agatgcacat gaaaatactg gtggtcgaag acgagatcaa aaccggggat tacctgcgac
    14581 aaggcctgac cgaagccggg ttcgttgtcg acctggcgcg tgatggcctt gatgggttgc
    14641 atctcgctca gaccgatgcc tatgacctgg cgatactgga tgtcatgctg cccggtatcg
    14701 acggctgggc ggtgttacag ggcattcgca gggccggcaa ggacatgccg gtattgttcc
    14761 tgacggcccg cgatcaggtg caggatcgtg tgcacggcct ggagttaggg gcggacgatt
    14821 acatggtgaa gcccttcgct ttcgccgaac tgttggcgcg cgtgcgtatc catctgcggc
    14881 gcggcagcaa gacgaaggag tccgagtatc tgcgcgcagc cgatctagaa ctcgacctga
    14941 tgcggcggcg cgtcacccgc gccggcaggc gaatcgacct gacggtgaag gagttcgctc
    15001 tgctcgaatt gctgttgcga cggcagggcg aagtgctgcc gcgctcgctg atcgcctctc
    15061 aggtgtggga catgaacttc gacagtgaca ccaacgttat cgaggtggcg atcaagcgtt
    15121 tgcggctcaa gatcgacgaa gacttcgaac tcaagctgat ccgcaccgtt cgtggaatgg
    15181 ggtatgtgtt ggagatcgcc gagtgaaatc agccggacgc aaatccatca ttttccgcct
    15241 gacgctgcta ttcgcttcgg catcgaccgc cgtcctgctt gtgcttgggc ttttgatcgg
    15301 caattcagtc gagcaccatt tcgaagtgca ggacatggag gtactgaccg gcaaactgga
    15361 actgacccgg cacgcgctgg aaaaggttca ttcgcaaagc gacctagatg ctttgccaca
    15421 gcaactggat gactccctgg tggggcatca tggcctggcg gtcattgtgg tggcgccttc
    15481 aggacaaacc ctgtttgtta ccagtggtgc cgagtttcca caggtcttgc ttgcgcgaag
    15541 ccctgatatg accaactcgg gtccgatcat ctggaaatcg acggatcaca taccgctgcg
    15601 cgggatttcg gcgatggtca aaaccggcat cgagggggcg aaacctgccg tcgttgccgt
    15661 agcgacggac atatcgaagc acgaacactt catgagttgg tttcgattca cattatggtc
    15721 tgtcgtgatt ctggctgcac tcatgaccgg atttctcggc tgggtggctg tcagacgcgg
    15781 gttggcgcca ttgcacgcga tcaagctgga ggcagcggcc attacagcgg atcgcctgca
    15841 ctccaggtta gctaccgaat cgatcccgat cgaactggtg gatcttgccg agactctgaa
    15901 ccagatgcta gcccgtctgg aggattcttt ccggcggttg tcggatttct cctcggatat
    15961 tgcccacgaa ctgcgaacgc cggtgagcaa tctgctgacg cagacccaag tcatgctctt
    16021 gcgtgtcagg acgattgacg aatatcagga tgtacttgcg tcaaacgcgg aggaactgga
    16081 gcgcatggcg aaaatgatcg ccgacatgct gttcctcgcc aaggccgaca ataacctcgt
    16141 ggtaccaaat ctcgaggccg ttgatcttcg ggcagaggcg gaaggattag cctcgttcta
    16201 cgaggctgtc gccgaggaaa acagcgttgc tctgactgta gacgggagcg ctaccgtaac
    16261 cggagaccgg ttgatgttgc gtagagccat cggcaacttg ctgtcgaatg cctttgggca
    16321 taccccccga ggaggttacg tacgaattca gatcggcaag aatgaagacc gaatggcaac
    16381 gatccaggtt gaaaactccg gcgagacgat ctcgccagaa catctgccgc gtctctttga
    16441 ccgtttctac cgggcagacc cttcaaggaa acgccttgcc gatggagttg gtctggggct
    16501 ggcgctcaca cgctcgatca ttcaagcgca tggaggatcc gtatcggttc ggtcggccga
    16561 tggggtcacc atatttgagc tgcaaatgta gccaagaccg cctttgatac agacttctgg
    16621 gttatggccg gtgtgtcgac cgcataccgg gcgtttgtgc atcaggggcg caacgataag
    16681 ttgctgcggc gttcataggg actgcacccg agatgttcgc caaagcttgt ccaggccagc
    16741 agcaccgaac tgcgacagga aatgcgttca ctgctttctc gttactgcca aacggaagca
    16801 gcccccgaag gagccgcttc acgatcagag gaatcggctg tttagcctca gcctcctttg
    16861 tgccctttgt tgatcaggga attcagacgc gcgacctcgt cactctgcgt cgtgattttc
    16921 cgtccatcac tggtctctac ctccgtgccc ggagtcgttc gagtggcacg accgtaccgg
    16981 tcctcgatcg ccattttccc atcattgaag atgtaaaggg ttgccccgtt tttcagatcg
    17041 acaacctgtt tcgcttcggc acgggcagca tcatcggcaa acgcggttgt cgcacctgag
    17101 gtgctcaaga ccgcaatcag ggccagtttg gcaagcttgg ttttcatggt ttactccttg
    17161 tgagatgggt tgattccatc gggcatatct cgtccgacgg tgatgccatc ttaggagacg
    17221 cataccaaca gggaggtgac caggaaatca cgtttcagtc agcattcgtt tggctatgag
    17281 gttttgttga ggtggctgaa tgaatcgccg ttgaatgaca gcataccgac ctcattgctg
    17341 acagattggg atcgcaggtt caaggtcagc tccggccgag ctgcagccgg cgcccaggtc
    17401 acagtgtccg tcagcttgcg gatcaggaag ccgccaaaga gcttgtaaac atcatcaggc
    17461 agcaatgggt cgttatgcaa agcgaccctt atggaaagct ttgcataagg gtcggtggca
    17521 gccggtggcc caaaaaatgt gaacgactgc ttcccgtctg acccggccgg tgatgctcgg
    17581 cctcggcggt ttcccatcga gctggtcgct ccaggaccac aagcggccac tgagttttcg
    17641 tggcttgctg tcggcaaccg acccatctcc agccgaatga ccggttacgg ttgatcgccc
    17701 cgaggtcggc aggccgcagc tcatgccgca tagctaccca ggcgatggcg aacggtctga
    17761 agcagcgctt cccgttcgtt gaggcaatcg tgcggggcac gagcacgctg gacttggcct
    17821 ggaccggcgc tggcgagcca accgctgggg aaatggaagc acgctgcgat ctcccacgtg
    17881 tcggcgacct cgccgagttg ggcgagaaca ccggcaatca ccggcagcgg ttgcaacgct
    17941 tcgctgaact ggtgggcagg gaaatactct ctcccgtcct agcttacggc gtagatcttg
    18001 cctgccttga aaggggaaac tgcggagagc atgctgcgtg cgcatcgatc gatgctgtcg
    18061 tgaaagccgg gatgtgccgt gcaatggagg aaaccggtca tggttctcat catctgaagt
    18121 gtcagctatg gctgttgggc aaggcaatgt gactggcaat tggtcatgtc ctcgcccagt
    18181 cggggcatca tccactcatg gttttccgca ttcgagaagg tagaatcacc gccatgatac
    18241 gctctgacct catcaccgtt ctcgcttcgc gcttccccag tcttgtggcg aaggatgccg
    18301 aaatcgcggt taaagagatc ctcgacgcca tcgggattgc tctggcacaa ggtgatcgcg
    18361 tcgaaatccg tggtttcgga agcttccgtt tgaattaccg cccggcacga acagggcgca
    18421 acccgaaaag cggagagccc gtgtcggttg ccgagaaata tgtgccgcac ttccaggccg
    18481 gcaaggaaat gcgcgagcgc gttgaacagt cggtcaagcc cgcgccactc cagcgggctg
    18541 cctgatgcgc gtccagcggt tgtttgcgct gatcgtgttg agcggaggac tggctttcca
    18601 gtgacggcca agcgccgatc gccatgccac cggcgcggcc tgttttcgca aaacgtggcc
    18661 atcgctgtcg ccgccgactt tgagcgtgtt gctcatggcc gtccgccgat gatgactttt
    18721 cgcgggacat atccggaggt attccacgca accgttgccg cctttgtcgt ctgcgccgag
    18781 ctaggctgca ccgttgccag cgccacaggc atcgacgagg gcagggcccc cactttcgtc
    18841 acgtccaccg tgacaaagcg ggcctccagc caatccgccc accttccggc cttgacaagc
    18901 agatccgccc ggtacaccgc gttgtactgc ggcgtccggg agtgataatt tgcggcccgc
    18961 gtccagaggt cgcccctgtc cttcttgatg tgctgccgca gccgcc
//
LOCUS       DAOSIT010000002        18112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_3317_length_18112_cov_10.919090, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000002 DAOSIT010000000
VERSION     DAOSIT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 18112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18112
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3317_length_18112_cov_10.919090"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLL14_00110"
     CDS             <1..898
                     /locus_tag="PLL14_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG02348.1"
                     /translation="RVPTSTEAAPPAAGRAANRQRFLWVDLRVASAEASVCEELATAF
                     QGIKVSEPPGIGEAITVHRPSFICFEFDQPDRRRLQTLQATKLYFPPLPILMLTEAHS
                     EALATWSFRSRVWDYLVKPVTFEELAWRARALYRLAETTRATGERHNLFPSQPVPFGE
                     RVGRSMPRTATAAASDYVRAHLDIGISLAKAASLCHLSVSEFSRVFKREHGRTFCDFL
                     LRSRVAKAWELLASPRVTVSEVAFAVGFNDLSYFTRIFRRYTGVSASEYRKASVQRVA
                     ETGSVFRPSGSEMPTSSLHPCL"
     gene            complement(901..1233)
                     /locus_tag="PLL14_00115"
     CDS             complement(901..1233)
                     /locus_tag="PLL14_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG02349.1"
                     /translation="MALSHAKSGDLVNTINASATLAGNTSVTLVRDDHIEIFRLVLGA
                     GTHMQEHSAAGAMTLQCLSGEVDLSCHGKTHTLQPGDLIYLNDAEPHALHCKQQAILL
                     VTLILHRK"
     gene            complement(1587..3692)
                     /gene="ptsP"
                     /locus_tag="PLL14_00120"
     CDS             complement(1587..3692)
                     /gene="ptsP"
                     /locus_tag="PLL14_00120"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG02350.1"
                     /translation="MSSTDNTQPAPLMIVGEVASSGLARGPAMLCDCARTQTAVPLRQ
                     VSAAEVQAEVERFDAAVLAAEGKLREVQESVRRTLGKDEAEIFEAQVLLLRDVQLRDA
                     VRALCLEKRMNVEAAVEEAIKQLMDLFGRLEDPYFRERATDLYDVGRRLLDHLAESGL
                     PDVPVVHEGCVIITSEILASVVARLEGQGVRGLIVEKGGLTAHATILARSLGIPTLVQ
                     APEATAKIRAGDLVIVDALAGRAFINPQADILRKYDQVDANLKAHQGVLKGLIGLPTV
                     THDGVEIKLCANIGQNADALAAANVKADGVGLYRTEFVFLVQDHFPSEDEQYQFYRAT
                     AEHLQPAETVIRVLDVGSDKPLSYFPLPREANPAMGFRGIRLLLAHPTLLHTQLRAIL
                     RLSASHPVAILLPMIDGIDELRAAKAAIEHAKEELAAAGQSFDPLIPVGAMIETPSAA
                     ILIEHLADEVDFFSVGSNDLVQFLLAADRIRGEMKSTYDPLHPAVIQVLAKLATVARR
                     KGTPISLCGEIASDPTYTNLLIGLGFRSFSVSPGRLLDIKHAIRSTDLQQAEKLAEQV
                     LSVHSTRDIRAQVQDDWNRRRPVASPEFNGPTGPVGTSLAVRHNVARQRFEAQAGESA
                     PAFLSYRGEGERVILKHTFVPDALRGQGIAADLVRVALEEARQRHWKIVPRCSYVAGF
                     IERHPEFCDLVDRQEGNLD"
     gene            complement(3689..5059)
                     /locus_tag="PLL14_00125"
     CDS             complement(3689..5059)
                     /locus_tag="PLL14_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG02351.1"
                     /translation="MDICCSDISAGNLPVSLYHSMIWLAGCNGKLPGRGVSQTSPGNK
                     VLIPRYPQAKTTQLNPMTIDPHTHGLSTLCIHAGTHLDQATGGACSPIFTSTAYAFPN
                     PANANIYPRYFNTPNQQVINRKLAALEKGEGALVFGSGMAAISTLLLANLKPGDHAIF
                     QNDLYGGSMRLISSELPRLGVQLSWGANVADFAEALRPETRLIYVESPSNPLLHCVDL
                     AEMAALAKRHGVLTVIDNTFATPINQNPLPLGIDAVIHSATKYLNGHSDVNAGVVVAS
                     AEIVSRVTGSAVNFGGMLDAHACSQLERGLKTLALRVQRHNENAETLARFLASHPAVS
                     KVNYPGLPEHPDHAIAARQMRGFGGMLSFELRQPEQLDRLLSRLRIVMPALSLGGVES
                     LICVPSRSTHRTMTPAERRRAGISDGLLRVSAGIEDVQDLIDDFTDALAGDDRRSPAS
                     TRESPL"
     gene            5037..5456
                     /locus_tag="PLL14_00130"
     CDS             5037..5456
                     /locus_tag="PLL14_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG02352.1"
                     /translation="MSEQQMSKVDFETLAHFRYQLRRYLRFSEDLTQRAGITPLQYLL
                     MLQIKGFPGRESANVGELAERLQAKHHGVVSLISRCEAAGLVQRSVSSSDRRQVDVQL
                     TRKGNECVEQLAGLHRRELLSLQGTFTVPDLTTLGRK"
     gene            5508..5969
                     /locus_tag="PLL14_00135"
     CDS             5508..5969
                     /locus_tag="PLL14_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG02353.1"
                     /translation="MQATAACADTDSDLYQAIVDQAPDVIIFADCEGAIRVWNHGAET
                     VFGHSAAEVLGESLDVIIPERLRKAHWEGFHKAVDSGQTKYRNQVLTTRSVHKDGSKL
                     YVELSFGLVRDGSGTIIGSLAIGRDCTARHLKAVAQSSAAAQDKPSDRALK"
     gene            6026..6514
                     /locus_tag="PLL14_00140"
     CDS             6026..6514
                     /locus_tag="PLL14_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG02354.1"
                     /translation="MLSACHGRIETQCVTLRRLVPHLLVHGVDEEARAAATRVIRYFD
                     TSALHHHADEEDDLFPALIESMAGSDAICLRAVIDGLKVDHRALEACWRHLRGILERI
                     AAGEQVSLVADDVEALAVRYACHIEYEEKELLPMAARLLSDHDLARIGRAMRERRGID
                     EA"
     gene            complement(6602..7306)
                     /locus_tag="PLL14_00145"
     CDS             complement(6602..7306)
                     /locus_tag="PLL14_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02355.1"
                     /translation="MPAAIEISGLTKRYGVRDAAVDALKGVDMIVQPGEVVGLIGPSG
                     SGKSTLLKCLGAVIEPSAGRMALAGEAIYDNGWQVADLRALRRDRIGFVFQAPYLIPF
                     LDVTDNVALLPMLAGVANASARERARAMLAALDVGHRSQARVAQLSGGEQQRVAIARA
                     LVNQPPIILADEPTAPLDSERALAVVRILNEMAQRFATAIIVVTHDEKIIPTFRRIYH
                     IRDGRTYEEAGEGRAA"
     gene            complement(7307..8500)
                     /locus_tag="PLL14_00150"
     CDS             complement(7307..8500)
                     /locus_tag="PLL14_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02356.1"
                     /translation="MINLAGRDILNGWGKFVFTGLGLGLLIGVTLSMAGIYRGLVDDG
                     RVLLGNAGADLWVVQRNTLGPYAEPSNVRDDAYRALLGVKGVVEAGNVTYLTMQIEDQ
                     GQDVRVMLVGFQPGHPGEPGYLVAGRPITRSHYEAIADIKTGFRLGQRIRIRRHEYTV
                     VGLTSRMVSSAGDPMVFVPLRDAQEVQFLKDNDALVNERARTAANPAFNRPGIPGLLE
                     AVQASQASSRTVNVVLLRLATHADEATVASNIRRWKHLEAYTRADMDEILVAKLIATS
                     AKQIFMFLVILAIVSAAIVAFIIYTMTLNKVREIAVLKLIGARNRTIAAMILQQALGL
                     GAIGFLVGKTAATLWAPIFPKYVLLLPGDAVRGALLVLAICALASVVAIRAALRVDPA
                     TAIGG"
     gene            complement(8497..9681)
                     /locus_tag="PLL14_00155"
     CDS             complement(8497..9681)
                     /locus_tag="PLL14_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG02357.1"
                     /translation="MNLRFSRKAVLRAVAVASVLLLGWLATSQGPLAPTRVTLTKVQL
                     GPLLESTFGIGTVEARRSYALGPTTASRVARVLVDQGDAVTAGQLLAELDPVDLDDRV
                     ASGRLVAQRAASAVRAAQAELVAAQSRALVAVASARRSAELRARGFFSQEAADAKQQE
                     ANAATAVADATAAQLAAARRDHERALADVAGVGKLRAQALLASPVDGIVSARLFEPGS
                     TIVAGQAVLQVIDPASLWVRARIDQGQAGGVRVGQSAEIVLRSDAKRVYPGEVRRVDW
                     LSDSVAEERIVNVAFAAIPDALSIGELVEVTIGTAELPDAPWLPTAAVKRVDRQDGVW
                     RIKDGRVTFHPVEVGISTLDGRSQILAGLAAGDEVVVHSEQALQPDAKVRVVVAIVGA
                     RR"
     gene            complement(9678..10313)
                     /locus_tag="PLL14_00160"
     CDS             complement(9678..10313)
                     /locus_tag="PLL14_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG02358.1"
                     /translation="MDIQSSVHKASRQRLSSDERQREIVAAVLALARERGPDAITTQA
                     IADRIGVTQGAIFRHFPHKEAIWMAVFAWVRESLGTLLATAVAQRQSPLAKIEAAFFA
                     HVAFIATNPGVPRVLFHELQYPGDSPVRAEVQAMIAGYRERLTALFTEARTAGELPAA
                     LDTALAPVLFIGAVQGLVIESALAGNEDAMMARAQQMFPLLVHGYRGPQEA"
     gene            10411..11016
                     /locus_tag="PLL14_00165"
     CDS             10411..11016
                     /locus_tag="PLL14_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02359.1"
                     /translation="MNFPEFFESAPRITVRDPLARFLGAVEDGIIEYTYSDAVKLAGH
                     SCPTVASAYLMTRAAMRALYPAAFPERGGIRVELRDDRLTGVAGVVANVVSLLTGASN
                     DTGFKGIAGRFDRRCLLSFGADIPGQLRFTRQDTSTAAIVSARLERLPGDARIASLMP
                     RCVAGTATAGQTAQFQSLWQDRVRTLLVEHADDPEIIVVQH"
     gene            11027..11530
                     /locus_tag="PLL14_00170"
     CDS             11027..11530
                     /locus_tag="PLL14_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02360.1"
                     /translation="MNIHRTLLTAALLTTFAIGAPASAAPASATPASATNSHQHAAAA
                     PTKLMLDHGKKWATDEPLRRNMSDIRTALVAKATAIHHGTLTADEYKALGSLVEAHIA
                     KIVTECKLDPAADENLHLIVAELTTGADAMQGKSKATPAAGAAQTVGAVNQYGRYFQH
                     PGWKPID"
     gene            11626..12780
                     /locus_tag="PLL14_00175"
     CDS             11626..12780
                     /locus_tag="PLL14_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014338858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOG02361.1"
                     /translation="MNDILQPDWDPKSDAVQRDQRAAYDEMRERCPVAYSDFLGWSVF
                     RHQDIVRVLMDPDTFSNAASRHLSVPDGMDPPEHTEYRRMIVQYFRPERVDAFEPQCR
                     EIAAKRLQSLLERDELEFIGDFAHWFAVRIQSASLGWPLEMCEPLRLWTQKNHQATFA
                     QDRTAMAEVAKEFEGYVHELLQIRRAAGAQASDDITGSLLRQQVQGQPLRDEEIVSIV
                     RNWTVGEVGTISAALGILVHYLSQHPELQQQLRTQPSLLPAATEEILRIYGPLVANRR
                     ITTRPVEIGGRKIAAGERISLIWISANRDGRVFEDPEAYRLDRDPTANLLYGAGIHVC
                     PGAPLARMEMRVVMEEILGRTTRIDPIPDRPPTGAVYPGSGFATLPIRLR"
     gene            12970..13098
                     /locus_tag="PLL14_00180"
                     /pseudo
     CDS             12970..13098
                     /locus_tag="PLL14_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(13119..14204)
                     /locus_tag="PLL14_00185"
     CDS             complement(13119..14204)
                     /locus_tag="PLL14_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOG02362.1"
                     /translation="MRVAPKIVLSEEERGELTKLSRSRRASVRLAQRARIVLLAADGM
                     RNDEIADQLGIGRVQVSRWRGRYAKSRLAGIERDLPRGAPPVKVDVARLVELTTQSTP
                     EAATHWSTRKMAAELGVSAASISRHWRANGLKPHLVRGFKVSRDPRFVEKLEDIVGLY
                     MSPPEHALVLCCDEKSQVQALDRTQPGLPLKKGRAATMTHDYKRNGTTTLFAALNVLD
                     GSVIAQCEQQHRHTEWLKFLRKIDRETPKDKALHLIADNYATHKHPVVQEWLAKHPRI
                     QMHFTPTSASWLNMVERFFRDITTERLRRGVFTSVPALVSAIDDYIAHHNTKPKPFIW
                     TKSARDILQKVIRANSRLSAKQNATLH"
     gene            complement(14282..15688)
                     /gene="ccoG"
                     /locus_tag="PLL14_00190"
     CDS             complement(14282..15688)
                     /gene="ccoG"
                     /locus_tag="PLL14_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOG02363.1"
                     /translation="MPQQPAPRKVIKITPVVDPDAKVYPRSISGPFTRWRWIFVWLTQ
                     IVFYGLCWVPWHGRQAILFDLETRKFYFFDLVLWPQDTIYLVLLMVLGALALFLFTAV
                     AGRLWCGYACPQTVYTEIFLWFEKIIEGERPQRIKLDAAPWSLRKLAIKTAKHAMWLL
                     FSLWTGFTFVGYFMPIRDLAQDLVTFSPGAWSSFWVVFYGFATYGNAGFLRDQMCRQI
                     CPYARFQFVMFDPDTLIIAYDVERGESRGARARGIDPRTQGLGDCVDCSICVQVCPTG
                     IDIRDGLQNECIGCAACIDACDQVMDKMNYPRGLIRYSTENAVSKRYTPMDIVRRAFR
                     PRVIIYSAILVLLVAATSWSLKTRVPLKVNILKDRNTLSREADDGSIENIYRLQIMNT
                     GDLTRTFRISIAGIENIHLDDDKTITVTGGEIGTRTAIVRTNPGAAKSGANPIRFSVE
                     DIDDPTVAVTELSKFWMP"
     gene            complement(15726..16127)
                     /locus_tag="PLL14_00195"
     CDS             complement(15726..16127)
                     /locus_tag="PLL14_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG02364.1"
                     /translation="MKRHPELLTLSREHHAALKLARDARRAAKSGEASELGNLAQRVV
                     SFFSAELDPHFRIEEKHLLVFLAHAGERDLVQRTLTEHAELRSIASTLCCPDPAMLLR
                     FADLLAEHVRFEERELFEVAQARLDGNPIQI"
     gene            complement(16161..16331)
                     /locus_tag="PLL14_00200"
     CDS             complement(16161..16331)
                     /locus_tag="PLL14_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02365.1"
                     /translation="MHLIIADLGTAAEALAGNDQKMSRQEGAMRLQNVLKTYGEFFDH
                     PNWRLSTSAHEH"
     gene            complement(16322..16654)
                     /locus_tag="PLL14_00205"
     CDS             complement(16322..16654)
                     /locus_tag="PLL14_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02366.1"
                     /translation="MSYQTRNWLIIAATLALNIGSAFAIDHAHDLEGYGATTLTLNNG
                     TKWPIDAPLRQGMTSIKAALLPQLPAIHENRLKASQYLRLAKQTNTQIGFMVTNCKLS
                     PDTTRNCI"
     gene            16762..17193
                     /locus_tag="PLL14_00210"
     CDS             16762..17193
                     /locus_tag="PLL14_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG02367.1"
                     /translation="MKLTTFSDYTLRVLMFLALNRDRLATIPEIAAAYDISENHLMKV
                     VHQLALAGVIETLRGKGGGIRLAREPEDIRLGQIIRTSEGSTPIVECLSDGGACRIAP
                     ACRLTAVLVRAFDALFATLDEYTLADLVCAPRSLNALLVRG"
     gene            complement(17507..17761)
                     /locus_tag="PLL14_00215"
     CDS             complement(17507..17761)
                     /locus_tag="PLL14_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02368.1"
                     /translation="MLKEQLQEAKSFAESFQARAGRAESEAAFWRGKAEAAAEESSRW
                     QRASEEAAGKIDGLQGRIRILEHELTERAPEESPTEKAAN"
     gene            17747..>18112
                     /locus_tag="PLL14_00220"
     CDS             17747..>18112
                     /locus_tag="PLL14_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02369.1"
                     /translation="MFLEHGFIRLCSLPDLIGLAHQVLTFAGGNFPLALDRFVRPLPF
                     GVHRLLVSVTFGEKFRLVITSLTLQGVGKPSAIFAHLADLLVTGPKEGDGIGQALPQV
                     FGLGLASLESRLKGLPLSLQ"
BASE COUNT         3404 a         5644 c         5579 g         3485 t
ORIGIN      
        1 gcgcgtgcct acgtcgaccg aggcggcacc gccagcggca ggccgcgctg ccaatcggca
       61 acgctttctg tgggtcgatt tgcgcgtcgc cagcgccgaa gcgagcgtct gcgaagagtt
      121 ggcgactgcc tttcagggca tcaaggtgag cgaaccgcca gggatcggtg aggcgatcac
      181 agtgcatcgc ccttcattca tctgcttcga attcgaccag cccgaccggc gccggctgca
      241 aacgctgcag gcgaccaagt tgtactttcc gccgctgccg attctcatgc tgaccgaggc
      301 ccactcggaa gcgctggcta cttggtcatt ccgctcgcgc gtctgggatt acctggtgaa
      361 gccggtcacc tttgaggaac tggcttggcg agctagggcg ctctatcgcc tcgcagaaac
      421 aacacgagcg acgggggagc ggcacaattt gtttccttcg caaccagtcc cgttcggcga
      481 acgggtcggc cggtcaatgc cacgcaccgc caccgcggcg gccagcgact acgtccgcgc
      541 ccacctggac atagggattt cgctggccaa ggcggccagc ttgtgccatc tgagcgtgtc
      601 cgaattcagc cgcgtcttca aacgcgagca cggccggacc ttttgcgatt tcctgctgcg
      661 cagccgggtg gccaaggctt gggagcttct ggcatcgccc cgggtgaccg tgtccgaggt
      721 cgcgtttgcc gtcggcttca acgacctgtc ctacttcacg cggattttcc gccgctacac
      781 cggggtttct gcgtccgaat accggaaggc aagcgtccag cgtgtggccg agacaggctc
      841 cgtgttcaga ccatcgggtt ctgagatgcc gacgtcatcg ctgcacccat gcctctgaag
      901 tcattttcga tgcaagatca gggtgacaag aaggatggct tgctgtttgc aatgtaatgc
      961 gtgcggctct gcatcgttca ggtagataag gtcgccgggc tgcagcgtgt gggttttacc
     1021 gtgacagctg aggtcgactt cgccgctcag acactgcaag gtcatggcac cggcagcgct
     1081 gtgctcctgc atatgggttc cggcgcccag gacgaggcgg aagatctcga tatgatcatc
     1141 gcgcaccagc gtcaccgaag tgttgccggc aagcgtggcg ctggcattga tggtgttcac
     1201 cagatcgccc gacttggcgt gtgacagtgc catggcaata ctcccctgac aaggcgacca
     1261 ttccgcggca gagccgggga cggtcggcat gtgagccgct catggcggca aaacggggac
     1321 gctaacacgg ccccaacaag ttcgcggcgc aacctgcgga ggagccgttc cgcccggatg
     1381 agccgggtga atggctcgga caatctaaag tggtccgacc agttttccca gatttcgatg
     1441 ctccgcaagc agttgcgaaa tgatctcgtt cacgcagtcc gccgttcttt gtgagccggc
     1501 attttcgggg aaaacagccc tcgtgaatcc cgccgcaacg actccatgga cccggcttca
     1561 acgcacccca gcccgaggca cttgcttcag tccaggtttc cttcctgccg gtcgacaagg
     1621 tcgcaaaatt cggggtggcg ttcaataaag ccggcgacat acgaacagcg cgggacgatc
     1681 ttccagtgcc gttgccgtgc ctcctcgaga gcgacacgaa ccagatcagc ggcgattccc
     1741 tggccgcgaa gcgcgtccgg cacgaaggtg tgcttcagaa tgacgcgctc gccttcgccg
     1801 cggtagctca gaaacgccgg ggcgctttcc cctgcctgcg cctcgaagcg ctgacgagca
     1861 acgttgtgtc tcaccgcgag cgaggtgccg acggggcctg ttgggccgtt gaactcgggc
     1921 gaagcaaccg ggcgacgccg gttccagtcg tcctgcacct gcgcccggat gtcgcgcgtt
     1981 gagtgcaccg acagaacctg ctcggcgagc ttttcagcct gttgcaggtc ggtcgagcga
     2041 atggcgtgct tgatgtccag cagccgtccc gggctgacac tgaaacttcg aaatccgaga
     2101 ccgatcaaga ggttggtgta ggtggggtca ctggcaattt cgccgcacaa ggagattggt
     2161 gttccttttc tcctggccac cgtcgccagc ttggcgagca cctggatgac tgccggatgc
     2221 aagggatcgt aggtggactt catttcacca cgaatccggt cggcagccag cagaaactgg
     2281 acgaggtcgt tgctgccgac actgaagaag tcgacctcgt cggccaggtg ctcgatgagg
     2341 atcgccgctg aaggcgtctc gatcatcgcc ccgaccggga tcagcggatc gaacgactgg
     2401 ccggctgcgg cgagttcctc cttcgcatgt tcgatcgccg ctttcgccgc acgcaactcg
     2461 tctatgccgt cgatcatcgg cagaagaatg gcgaccggat gcgaggcact caggcgaagg
     2521 atggcccgca gttgcgtgtg cagcagggtg ggatgcgcga gcagcagcct gatgccccga
     2581 aagcccatgg ccggattggc ttcgcgcggc agcgggaaat agctcagcgg tttgtcactg
     2641 ccgacatcga gaacgcggat caccgtttcc gccggttgca ggtgctccgc cgtggcgcgg
     2701 tagaactggt actgctcgtc ttccgacggg aaatgatcct ggaccaggaa gacgaactcc
     2761 gttcgataca gtccaacccc atcagccttg acattcgccg ctgccagcgc gtcggcattc
     2821 tggccgatgt tggcacagag cttgatttcg acgccatcgt gtgtgaccgt cggcagacca
     2881 atcaagccct tcaggacgcc ttggtgagct ttcaggttgg cgtccacctg atcgtacttc
     2941 ctcaggatat cggcctgagg attgatgaag gcgcgtccgg ccagagcatc gacgatcacc
     3001 agatcgcccg cacggatctt cgccgtcgct tccggcgcct gtacgagcgt ggggatgccg
     3061 agcgagcggg caaggatggt ggcgtgcgcc gtgaggccgc ctttctctac aatcaagcca
     3121 cgcacgccct ggccttccag gcgagcaacg acggaggcga gaatttcgct ggtgatgatc
     3181 acgcatccct cgtggacaac cggaacatcc ggcagtccgc tttccgccag gtgatcgagg
     3241 aggcgtctgc cgacgtcgta cagatcggtg gcacgctcgc ggaagtaggg atcttcgagg
     3301 cggccgaaca agtccatcaa ttgcttgatc gcttcttcga ccgccgcctc cacattcatc
     3361 cgcttttcga ggcaaagcgc acgcaccgca tcgcgcagtt gcacgtcgcg caggagaagg
     3421 acttgcgctt cgaaaatctc cgcctcgtcc ttccccaaag tccgccggac gctctcctga
     3481 acttcccgca gcttgccctc ggcggcaagc accgcggcat cgaaacgctc gacctcagct
     3541 tgcacctccg ccgcgctcac ctgccggagc ggaacggctg tctgcgttcg ggcgcaatcg
     3601 cacagcatgg cgggtccgcg ggcaaggccc gacgacgcca cttcgccgac gatcatcaat
     3661 ggggcgggct gcgtgttgtc agtgctgctc ataagggaga ttccctcgtg gaggccgggg
     3721 aacgacgatc atccccggcc agtgcgtcgg tgaagtcgtc gatcaggtct tgcacatcct
     3781 cgatgcctgc agagacgcgt aacagaccat cgctgatgcc ggcacgccgt cgctcggcag
     3841 gcgtcatggt gcggtgtgta ctgcggctgg gaacgcaaat caggctttcc acgccgccca
     3901 gactcagggc cggcatgacg atacgcagcc ggctcaacaa gcgatccagc tgctctggtt
     3961 gacgcagctc gaacgacagc atgccgccaa acccgcgcat ctggcgtgcc gcgatggcgt
     4021 ggtcgggatg ttcgggcagg ccgggataat tgactttgga caccgccgga tgcgaggcaa
     4081 ggaaacgtgc cagcgtctcg gcattctcat tgtgccgttg cacgcgcagg gccagtgtct
     4141 tcagtccacg ttccagttga gaacaggcgt gcgcatcgag cattccgccg aagttgacgg
     4201 cactccccgt cacgcggctg acgatttcag cggaggcaac gaccacgccc gcgtttacgt
     4261 cgctgtggcc gttgaggtac ttggtagcac tgtggatgac cgcatcgata cccagcggaa
     4321 gcgggttctg attgatcggc gtggcaaaag tgttgtcgat gacggtcaaa acgccgtggc
     4381 gtttcgcaag cgcagccatt tcggccagat caacgcaatg cagcagcgga ttcgacggag
     4441 attcgacgta gatcagccgc gtctccggtc gcaaggcctc ggcgaagtcc gccacgttcg
     4501 ccccccagga aagctgcacg ccgagccgcg gcagttcgct gctgatcagc cgcatactgc
     4561 cgccgtagag atcgttctgg aagatggcgt gatcgccagg tttgagatta gcaaggagca
     4621 aggtggagat cgccgccatg cccgagccga agacaagcgc cccctcgccc ttttcgagcg
     4681 ctgccagctt gcggttgatg acctgctggt tgggcgtatt gaagtaacgc gggtagatgt
     4741 tcgcgttggc cggattcggg aacgcgtagg ccgtggaagt gaagatgggg ctgcacgcgc
     4801 cgccggtggc ctggtccaga tgcgtgccgg cgtgaatgca gagtgttgac aggccgtggg
     4861 tatgtgggtc gatagtcatc gggtttagtt gagtcgtctt tgcctgtgga tacctcggga
     4921 taaggacttt attgccagga gaagtttgcg aaacaccgcg cccaggcaac ttgccattgc
     4981 atccagccaa ccatatcata ctgtgataca aacttacggg aaggttccct gcagaaatgt
     5041 ccgagcagca aatgtccaag gtcgatttcg aaacgcttgc ccacttccgc taccagctgc
     5101 gccgctacct gcgcttcagc gaagatctga cgcagcgggc cgggataacc cccttgcagt
     5161 atctgttgat gctccagatc aaaggctttc ccggacggga atcggccaac gtcggcgaac
     5221 tcgccgaacg cctgcaggcg aagcatcatg gggtcgtgtc gctgatttct cgatgcgagg
     5281 cggccggact agtacagcgt agcgtcagca gcagcgaccg gcggcaggtg gacgtgcaac
     5341 tgacaagaaa aggcaacgaa tgcgttgagc aactggctgg ccttcatcgc cgtgagctcc
     5401 tgtccttgca gggaacattc acggtgcccg acctaaccac gctcggccgc aaatagcaac
     5461 gacggcgagc gcttgtctca ttcgaccaag tggaaaggag ttccaggatg caggcaactg
     5521 ccgcgtgtgc ggatacagat tccgatctct atcaggcgat cgtggatcag gccccggatg
     5581 tgatcatttt cgccgattgc gagggagcga tccgcgtctg gaatcatggc gctgaaaccg
     5641 tgttcggcca ttccgccgcc gaagtcctcg gcgaaagcct cgacgtgatc attccggaac
     5701 ggctccggaa agcacactgg gaaggctttc acaaagcggt tgacagcggc cagaccaagt
     5761 accggaacca ggtactcaca acccgctcgg tgcacaagga tgggagcaag ctctacgtgg
     5821 aactgagctt cggcctggtg agggacggca gcggcacgat catcggttcc ctggccatcg
     5881 gccgcgactg taccgcccgc cacctcaagg cagtcgcaca gtcttcggct gccgcgcaag
     5941 acaagccgag cgacagggcc ttgaagtgac gacaagcatt cctggattct ccgcaccgac
     6001 cgtcggaacc gaggcgcctc tggagatgct ttccgcctgc catggcagaa ttgaaacgca
     6061 atgcgtgacc ttgcggcgcc tggttccaca cctgctggtt catggcgtcg acgaagaagc
     6121 gcgtgcggcg gcgaccaggg taatacgcta cttcgacaca tccgccctgc accatcatgc
     6181 cgatgaagaa gacgacctct ttccggcgtt gatcgagtcg atggccggtt ctgacgccat
     6241 ctgcctgcgc gcggtgatcg acgggctgaa agtcgatcac cgcgcgctcg aagcgtgttg
     6301 gcgacacctg cgcggcatcc tcgaaaggat cgccgccggc gagcaggtgt cactggtcgc
     6361 tgacgatgtc gaagcgctgg ccgttcgcta tgcgtgccat atcgagtacg aggaaaagga
     6421 gctcctgccc atggctgcgc gcttgttgag cgaccatgat ctggcccgca tcggacgcgc
     6481 gatgcgcgag cgccggggaa tcgacgaggc ttaggcgagg tttaggcaac aagctcgcgc
     6541 acgcgagcgc aaatcccggc tgccgacgat cgccgagcgt ctctttctcg tcctccgcgg
     6601 atcaagcggc ccgcccctcg ccggcttcct cataggtgcg tccgtcacgg atgtgataaa
     6661 tccgccggaa ggtcgggatg atcttttcgt cgtgggtgac gacgatgatc gctgtcgcga
     6721 agcgctgtgc catctcgttg aggatgcgga ccacagccaa tgcgcgctcg ctgtccaggg
     6781 gggccgtcgg ctcgtcggcg aggatgatcg gcggctggtt aaccagcgcg cgagcgatcg
     6841 ccacccgctg ctgctcaccg ccggacagct gcgccacccg tgcttgggaa cgatgcccga
     6901 catcgagtgc cgcaagcatc gcccgcgccc gctcgcgcgc actggcgttg gccacaccgg
     6961 ccagcatcgg tagcaacgcc acgttatcgg tgacgtcgag gaaaggaatg agatacggcg
     7021 cctggaagac gaagccgatg cgatcgcgac gcaaggcgcg cagatcggcc acctgccagc
     7081 cattgtcgta gattgcctcg ccggccagcg ccattcgccc cgcgctgggc tcgatcaccg
     7141 cacccaggca cttgagcagc gtgctcttgc ccgagccact ggggccgatc agcccgacaa
     7201 cctcgccggg ctggacgatc atgtccacgc ccttcagggc atcaaccgct gcatcgcgga
     7261 cgccgtagcg cttggtcagg ccgctgattt cgatcgccgc gggcatctat cccccgatcg
     7321 ccgtcgctgg atcgacgcgc aacgcggcgc ggatggcgac gacgcttgcc agcgcgcaaa
     7381 tcgccagcac caacagcgca ccgcgcacgg cgtccccggg gagcagcagg acgtacttgg
     7441 ggaagatcgg tgcccacagc gtcgccgcag tcttgccgac gagaaaaccg atcgcaccca
     7501 ggccgagcgc ctgctgcagg atcatggcgg cgatggtccg gttgcgtgcc ccgatcagct
     7561 tgagcaccgc gatctcgcgg accttgttca gggtcatcgt gtagatgatg aaggcgacga
     7621 tggccgcgct gacaatcgcc aggatgacca ggaacatgaa gatctgcttc gccgacgtgg
     7681 cgatcagctt ggcgacgagg atttcgtcca tgtcggcacg cgtataggct tcgaggtgtt
     7741 tccagcgccg gatgttgctg gctacggtcg cttcatcggc gtgcgtggcg agccgcagca
     7801 ggaccacatt caccgtccgg ctgctcgcct ggctggcttg tacggcctcg agcagtcccg
     7861 gtataccggg gcggttgaac gccggattgg cggcagtgcg tgcgcgttca ttgaccaacg
     7921 catcgttgtc cttgaggaac tgcacttcct gcgcatcgcg cagcggcacg aagaccatcg
     7981 ggtcaccagc cgacgacacc atgcggctgg tcaagccgac caccgtgtac tcgtgccgtc
     8041 ggatacggat gcgctgccca aggcggaagc cggtcttgat atcggcaatc gcctcgtagt
     8101 gcgaacgcgt gatcggacgt ccggcgacga ggtagccggg ctcaccgggg tgtccgggct
     8161 ggaagccgac gagcatcacc cgtacatcct ggccctgatc ttcgatctgc atcgtcaggt
     8221 aggtgacatt gccggcctcg accaccccct tgacgccaag cagcgcgcga taggcgtcgt
     8281 cgcgtacgtt cgagggttcg gcgtaggggc cgagcgtatt gcgttgcacg acccatagat
     8341 cggccccagc attgcccagc aggacgcgtc cgtcatcgac cagtccgcga tagatcccgg
     8401 ccatcgacag cgtgacgccg atcagcaagc cgagcccgag gccggtgaag acgaacttgc
     8461 cccagccgtt gaggatgtcg cgaccggcga gattgatcat cgcctggcac cgacgatggc
     8521 gacgacgacc ctgactttcg cgtcgggctg caatgcctgc tcgctgtgta cgacaacttc
     8581 gtcgcctgct gccagaccag caagaatctg gctgcggcca tccagggtgg agatgccgac
     8641 ctcgaccgga tgaaacgtga cgcgcccgtc ctttattcgc cacacgccat cctgtcggtc
     8701 cacacgcttc accgctgccg tcggcaacca gggtgcatcg ggcagttcag cggtgccgat
     8761 ggtcacctcg accagctctc cgatcgagag tgcgtcggga atcgccgcga aggcgacatt
     8821 gacgatgcgc tcttcggcga ccgaatcgct tagccaatcg acgcgccgga cttcgcccgg
     8881 gtagacacgc ttggcatccg agcgcagcac gatctcggct gactgtccaa cgcgcacgcc
     8941 gccggcctgg ccctggtcga tgcgcgccct gacccacagg ctcgccgggt caatgacctg
     9001 cagcacggcc tgcccggcaa cgatcgtcga tcccggttcg aacagtcgcg cactgacgat
     9061 gccatcgacg ggactcgcca gcagtgcctg cgcgcgcagc ttgccgacac cggcaacatc
     9121 ggccagcgcg cgttcgtgat cgcgccgcgc cgccgccagt tgggcggccg tggcgtccgc
     9181 taccgccgtc gcggcattgg cttcttgctg cttggcgtcg gcggcctctt ggctgaagaa
     9241 accgcgtgcg cgtagttcgg cggaacgacg cgcgctggcg accgcgacta gcgcacggct
     9301 ctgcgccgcg accagctcag cttgcgccgc gcggacagcg cttgcggcac gttgtgcgac
     9361 gagtcggccg ctcgcaacgc gatcgtcgag gtcgaccgga tcgagttcgg caagtagttg
     9421 cccggccgtg acggcatcgc cctggtcgac cagcacgcgc gccacgcggc tggccgtcgt
     9481 cggacccagt gcatagctgc ggcgagcctc gactgtgccg atgccgaagg tgctctccag
     9541 cagcggcccc agctgcacct tggtcaaagt caccctggtt ggcgccagcg gtccctggct
     9601 ggtcgccagc cagccgagca acagtacact ggcgacggcg actgcgcgca ataccgcctt
     9661 gcgggaaaag cgcaagttca tgcttcttgt ggcccgcgat agccgtgcac gagcagcggg
     9721 aacatctgct gcgcacgcgc catcattgcg tcctcgttgc cggcgagcgc tgactcgatg
     9781 accaggccct gcaccgcccc aatgaacagc accggcgcca gcgcggtatc cagcgcggcg
     9841 ggaagctcac cggccgtcct ggcctcggtg aacagcgcgg tcagccgctc gcggtagcca
     9901 gcgatcatcg cctggacctc ggcgcgcacc ggcgaatccc cgggatattg cagctcgtgg
     9961 aacagtacgc ggggaacgcc ggggttggtc gcgatgaagg ccacgtgcgc gaagaacgcg
    10021 gcctcgatct ttgccagcgg cgactggcgc tgcgccacag cggtcgccaa aagcgtgcca
    10081 agcgattccc ggacccaggc aaagaccgcc atccagattg cctccttgtg cgggaagtga
    10141 cggaagattg ccccctgcgt gacgccgatc cggtcggcga tggcctgcgt ggtgatcgca
    10201 tccggcccgc gctcgcgggc cagcgcaagt accgcagcga cgatctcgcg ctgtcgttcg
    10261 tcgctgctca gacgttggcg agaagccttg tggacgctgc tctgaatatc catgatggtg
    10321 gtcagcttgc atttctgtaa gtaagtggtt actatcttag gcgtgtagtg tccgtttggc
    10381 aagcgcgact gtcccgagga gaacgaactc atgaatttcc cggagttttt tgaatctgca
    10441 ccacgaatta ccgttcgcga cccactggcc cggttcctcg gggcggtgga ggacggcatc
    10501 atcgaataca cctacagcga cgcggtcaag ctcgccgggc attcgtgccc gaccgtcgct
    10561 tccgcctatc tgatgacccg cgcagccatg cgcgccctct acccggcagc ctttcccgag
    10621 cgcggtggca tccgcgtcga gctccgggat gaccgactca ccggggttgc tggcgtcgtt
    10681 gccaatgtcg tcagcctgct caccggtgcg agcaacgata ccggcttcaa gggaattgca
    10741 gggcgttttg atcgccgttg cctgctctcc ttcggcgccg acatccccgg ccagctgcgt
    10801 ttcacgcggc aggacaccag tacggcggcc attgtctccg cccggctgga gcggttgccg
    10861 ggcgatgcgc gcatcgcttc cctgatgccg cgctgcgtcg ccggtactgc caccgccggg
    10921 cagaccgcac agttccaatc actatggcaa gaccgcgtcc ggacgctgct cgtcgagcat
    10981 gctgacgatc cggagatcat tgttgttcaa cattgaggag aatcccatga acatccaccg
    11041 caccctgctc acagccgccc tgctcactac cttcgccatc ggcgcgcctg cctctgccgc
    11101 ccccgcctct gccacccctg cttcggccac caattcgcac cagcacgcag cggctgcgcc
    11161 aaccaaattg atgctcgacc acggcaagaa gtgggcaacg gacgagccgc tgcgccggaa
    11221 catgagcgat atccgcaccg cgctggtcgc gaaagcgaca gccatccacc acggaacgct
    11281 gacggcagac gagtacaagg cgttgggcag cctggtcgag gcgcacatcg ccaagatagt
    11341 caccgagtgc aagctcgacc ccgcagcaga cgaaaacctg cacctcatcg tcgccgaact
    11401 cactaccggt gccgacgcga tgcagggcaa gtcgaaagcc acgccggctg ccggagcagc
    11461 gcagaccgta ggcgcggtga atcagtacgg ccgttacttc cagcacccgg ggtggaaacc
    11521 aatcgactga ttcgtctggc gagcaaacaa tgaatggctg cgcggccggc ctttcatcgt
    11581 cgagcgcggg ggccgctact ctaagacgat gaggaaggaa cgaccgtgaa cgatatcttg
    11641 caaccggatt gggacccgaa atccgacgcc gtccagcgtg atcagcgagc tgcctacgac
    11701 gagatgcgtg agcgctgtcc ggttgcgtac agcgactttc tcggctggtc cgtgtttcgg
    11761 caccaggata tcgttcgcgt gctgatggat cccgacacct tcagcaacgc cgcatcccga
    11821 catctctccg ttccggacgg catggatccc cccgagcata ccgagtaccg acggatgatc
    11881 gtgcaatatt ttcggccgga acgtgtggac gcgttcgagc cgcaatgtcg cgagatcgcc
    11941 gcgaagcgtc ttcaatcgct gctggagcgc gatgagctgg agttcatcgg cgacttcgcc
    12001 cattggttcg ccgtacggat tcagtccgcc tcgctcggtt ggccgctcga aatgtgcgaa
    12061 ccgctgcgtc tctggacgca gaagaatcat caggcgactt ttgcgcagga ccggacggcg
    12121 atggcggagg tcgccaagga attcgagggc tacgtccacg agttgctgca gatccggcgg
    12181 gcggccggtg cgcaggccag cgatgacatc accggttcac tgcttcggca acaggtacag
    12241 ggccaaccgt tgcgcgatga agaaatcgtc agcatcgtgc gaaactggac cgtcggtgag
    12301 gtcggcacga tttcggcagc gctgggcatc ctggttcact acctctcgca acacccggaa
    12361 ctgcagcagc agctccgcac gcagccctcc ctgctgcccg ccgcaaccga agagattctc
    12421 cggatctacg gaccattggt cgccaaccgc cgcatcacca ctcgtccggt agagatcggt
    12481 ggcaggaaga tcgccgccgg cgaacggatc tcgctgatct ggatttctgc caaccgcgat
    12541 ggtcgtgttt tcgaggatcc ggaggcgtat cggctggacc gcgacccgac cgcaaacctg
    12601 ctatatggcg cgggaatcca cgtctgccca ggcgctcctc tggctcggat ggagatgcgt
    12661 gtggtgatgg aggagatcct cgggcgcacg acccgcatcg acccgatccc ggaccggccg
    12721 cccaccggcg cggtgtaccc cggcagtggt ttcgccaccc tgcccatacg actccgatag
    12781 ggggggctac gccggaaccc cagaagattc cgtcatcggg ttctgagatg cagtggagcc
    12841 gaaaaagcag ttaagaggcc aaaatagatg gtcaaatagg gcgtagccaa tttacattgg
    12901 ctcttttgtt cttgaccaca tcttcaaaag atagccaagt tcggtatacc caacttggcc
    12961 atccactaca tgcaaatcgc ccgactctac ctgcgtgtca gcaccgacga acaagacctt
    13021 gcgcgccaag ccgacatcga gcacagcgcc cgtcaggctg gctattacat cgccggcatc
    13081 tatcgcgaga aggccaacac ccgatcatgg acggcccgct agtgtagtgt tgcattctgt
    13141 ttagcgctta agcggctgtt ggcgcgaatg accttctgga ggatgtcgcg agcgctcttg
    13201 gtccagataa agggcttggg cttggtgttg tgatgggcga tgtagtcgtc gatggcagag
    13261 accaacgcgg ggacgctggt gaacacgcca cggcgcagtc gttcggtcgt gatatcgcgg
    13321 aagaagcgct cgaccatgtt cagccaggag gccgaggtgg gggtgaaatg catctgaatc
    13381 cgcggatgct tggccaacca ttcctgtaca acgggatgct tgtgtgtggc gtagttgtcg
    13441 gcgatcaggt gcagcgcctt gtccttcggc gtttcccggt cgatcttgcg aagaaacttg
    13501 agccactctg tgtgacggtg ctgctgttcg cactgtgcga tcacgctgcc gtccagcacg
    13561 ttgagcgcgg cgaacagcgt ggtcgtgccg ttgcgcttgt aatcgtgggt catggtggct
    13621 gcacgccctt ttttcagcgg cagcccgggc tgcgtacggt cgagtgcctg cacctggctt
    13681 ttctcatcgc agcacaacac cagcgcatgc tcgggtggcg acatgtacag accgacgatg
    13741 tcttcgagtt tctcgacgaa cctggggtca cgagagacct tgaagccacg taccaggtga
    13801 ggcttcaagc cgttggcgcg ccagtggcgc gagatgctgg ccgcactgac gcccagttcg
    13861 gcggccatct tgcgggtact ccagtgcgtc gctgcttcgg gtgtgctttg cgtggtcagt
    13921 tccaccagtc gcgccacatc caccttcacc ggaggggcgc cgcgcggcaa gtcgcgctcg
    13981 attcccgcca gccgcgactt cgcataacgc cctcgccaac gcgacacctg tacgcggcca
    14041 atccccaact gatccgcgat ctcatcgttt cgcatcccat cagcggccag cagcacaatc
    14101 cgcgcacgct gtgccagccg cacgctggcg cgcctggaac gagaaagctt ggtcaactcg
    14161 ccacgctcct cttcggacaa gacaatcttc ggggcaactc gcatctgcat ctccactccg
    14221 cacacaacgt gcattggaga cgtggtatcc agacaagttc cctcaagaat gcaacactac
    14281 actagggcat ccagaacttt gagagttcgg taacggcaac ggtaggatcg tctatatcct
    14341 cgacactgaa cctgatcggg ttggcgccag attttgccgc tccgggattg gtcctgacaa
    14401 tggcggttcg ggtaccgatt tcgccgcctg tcaccgtgat cgttttgtcg tcgtcgaggt
    14461 gtatgttttc aatgccggca atgctgatcc ggaaggtccg ggtcaaatca cctgtgttca
    14521 tgatctgcag tcggtagatg ttttcgattg acccatcgtc agcttcgcgt gagagcgtgt
    14581 tgcggtcctt cagtatattg actttgagcg gtacgcgcgt tttcagtgac caagaggtcg
    14641 ccgccaccag caagacaagg atcgcgctgt agatgatcac tcgtggccga aacgctcgcc
    14701 gcacgatatc catcggcgtg tagcgcttgc tgaccgcgtt ctccgtggag tagcggatca
    14761 ggccgcgagg ataattcatc ttgtccatca cttgatcgca ggcgtctatg caggcggcgc
    14821 agccaatgca ctcattctgc aaaccatccc ggatgtcgat tccggtggga caaacctgga
    14881 cgcagatgct gcagtcgacg caatcgccga ggccctgtgt tcgcgggtca atacctctgg
    14941 cacgtgcccc acgcgactcg cctcgttcga catcgtaggc gatgatcagg gtgtcgggat
    15001 cgaacatgac aaactgaaat cgcgcgtatg ggcaaatctg tcgacacatc tggtcgcgca
    15061 ggaagccggc attgccatac gtggcgaagc catagaaaac tacccagaag gatgaccatg
    15121 cgccaggcga gaaggtgaca agatcctgcg cgagatcgcg aatcggcatg aaatagccga
    15181 cgaaagtgaa accggtccac agggagaaga ggagccacat ggcgtgcttg gccgtcttga
    15241 tggctagctt gcgtaacgac cacggcgcgg catcgagctt gatgcgctgc ggccgttcac
    15301 cttcgatgat tttctcgaac cacaggaaga tttccgtata caccgtttgc ggacaggcat
    15361 agccgcacca caggcgtccc gctaccgcgg taaacaggaa cagggcgagc gctccgagaa
    15421 ccatcagcag aacgagatag atcgtgtcct gcggccacag taccagatcg aagaagtaga
    15481 atttgcgcgt ctcgagatcg aacaggatgg cctgccgacc gtgccatggc acccagcaaa
    15541 ggccgtagaa aacgatctgc gtcagccaga caaaaatcca gcgccagcga gtgaatggcc
    15601 cgctgatgga gcgcgggtaa accttggcat ccggatcaac gaccggtgtg atcttgatga
    15661 cttttctggg ggctggctgc tggggcattt tttttccgac ggccgcaatc cgaatggatt
    15721 gcgcctcaaa tttgaattgg attcccatcg agcctggcct gcgccacctc gaatagttcg
    15781 cgctcctcaa agcggacatg ttccgccagc agatctgcaa atcgcaacaa catggctgga
    15841 tcggggcaac acagtgtgct ggcgatactg cgcaattctg catgttcagt cagcgtgcgc
    15901 tgaaccaggt cacgttctcc ggcatgcgcc agaaacacca gaaggtgctt ctcctcgata
    15961 cggaagtgcg gatcgagttc ggccgagaag aaactgacga cgcgttgtgc cagattcccc
    16021 agttcgctcg cctcgccaga tttcgcggca cgtcgggcgt cacgcgcgag cttgagcgcg
    16081 gcgtgatgct cacgggaaag cgttagcaac tccggatgcc gcttcatgct tgagatctgt
    16141 ccgccaaatg atgtcgccga tcagtgttca tgcgcggaag tggacagcct ccagttggga
    16201 tgatcgaaga attcgccgta ggttttaaga acgttctgca gccgcattgc gccctcctgg
    16261 cgcgacatct tctggtcgtt tcccgccaag gcttcggcgg cggtgccaag atcggcgatg
    16321 atcagatgca attgcgcgtt gtgtcaggcg aaagcttgca attggtgacc ataaagccaa
    16381 tctgcgtgtt cgtctgtttt gccaatcgca ggtattgaga agccttcagc ctgttttcgt
    16441 gaatcgcagg taattgcggt agcagtgcgg ccttgatact cgtcattccc tggcgcaatg
    16501 gagcgtcgat cggccacttc gtgccgttgt tcaatgtcag cgtggtggca ccgtagcctt
    16561 cgaggtcatg cgcgtggtcg atggcgaagg ccgaaccgat attcagcgct agagtggcgg
    16621 cgatgatcag ccagttgcga gtttggtatg acatggcgaa cgtccttgag aaagcggtat
    16681 aattaatatt gcttcatcgt aacaccctct ttttcgtaaa gcaatatagt ttatacagct
    16741 tatgatcgct cacacgctgg cgtgaaactc actaccttct ctgactacac cctgcgcgtc
    16801 ctcatgttcc tggcgctcaa tcgtgaccgg ctcgccacca ttccggagat cgccgccgcc
    16861 tacgacatct cggagaatca cctgatgaag gtggtgcatc aactggcgct ggcaggggtc
    16921 atcgaaactt tgcgagggaa aggtggcggc atccgcctgg cgcgcgagcc tgaggacatt
    16981 cggctggggc agatcattcg caccagcgaa ggcagtacgc ccatcgtcga atgcctgtcc
    17041 gacggcggtg cctgtcgcat cgcaccggct tgccgactga cggcagtact ggtccgcgcc
    17101 tttgatgcgc tcttcgcgac gctggacgaa tacacgctgg ccgatctagt gtgtgcgccg
    17161 cgcagcttga atgcattgct ggtccgcggc taaccaggaa gtggcaaccc ggcagatcat
    17221 tatgttctgg ctgatggcac cttgagtgtg aagcaggttt ccaagccgct ggagcggttt
    17281 cggcgagttc gcacgcaaaa caccgtattt ttctggcgcg ctcatcttgg ttgttggcct
    17341 ttacctccgc gctggcctga ttggtagcag gctatcgagg aagcattgcg acgggttccc
    17401 ttgtccatcc ttggtcattt gcagtgtgcc gctggccagg tacggcacct gttggagaac
    17461 gatcacgccc gtgcctggga agaactcaac ccggaatagt cgctgctcag ttcgccgcct
    17521 tttccgtagg agactcctct ggtgctcgct cggtgagttc gtgctccagg atccgtatgc
    17581 gcccctgcaa gccatcgatt ttcccggccg cttcctctga cgctcgctgc cagcggctgg
    17641 actcttccgc cgccgcttcg gccttgccgc gccagaacgc cgcttcgctt tctgcccggc
    17701 cggctcgcgc ctggaagctc tcggcgaagg atttggcctc ctggagttgt tccttgagca
    17761 cggcttcatt cgcctttgca gcttgccgga tctcatcggt ctggcgcatc aggtactgac
    17821 gttcgccggc ggcaatttcc cgctcgcgct cgaccgattc gtgcgcccgc tgccgttcgg
    17881 cgttcaccgc ctgctcgtgt cggtcacgtt cggcgagaag ttccgcctcg tgattacgtc
    17941 gctcactctc cagggtgttg gcaagccgag cgccatcttc gcgcatctgg ccgatctgct
    18001 cgtcacgggc ccgaaggagg gtgatggtat cggacaggcg ttgccgcaag tcttcggtct
    18061 cggcctggct tccctggagt ctcgcctgaa gggcttgccg ctctccctgc aa
//
LOCUS       DAOSIT010000003        17817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_3383_length_17817_cov_8.032598, whole genome shotgun sequence.
ACCESSION   DAOSIT010000003 DAOSIT010000000
VERSION     DAOSIT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 17817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17817
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_17817_cov_8.032598"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..1495)
                     /locus_tag="PLL14_00225"
     CDS             complement(320..1495)
                     /locus_tag="PLL14_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG02370.1"
                     /translation="MAATDLISAAILIATDDVTDAEMVRKNLAEEFKNVFVSTNPDLI
                     AQDFERRNPDVLVLAFATLEKAERYYLGLYRLYPAVQQHLHRTVILCNKDEVKRVYEL
                     CKKSYFDDYVLFWPMNYDGSRLPMSIHRALHELSVIKGGGPSVAEFAAQARRLAELEK
                     QLDAQMATGNRHIDVASRAMQQAEQDVGAALDGFSRRLIAGALPGAVTVNNAGALQDE
                     IGRLKREDVMERFRTAAKSVAPLKQWADDFKQEFSTHMASVRELNVLADSVRPTVLVV
                     DDDELQQKLVAKILAAENYQLIFAAKGVEALAVMRKTRPDVILMDFKMPEMDGIETTR
                     RLKAVPQFAKLPVIMITGQSEGSVVVDSLKAGACGFLVKPFARDTLIAKLRQVLNGA"
     gene            complement(1495..2730)
                     /locus_tag="PLL14_00230"
     CDS             complement(1495..2730)
                     /locus_tag="PLL14_00230"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HOG02371.1"
                     /translation="MTNNFAVKIMLLDDEPFMLKLIGRQLENLGFTALSPYTSGAAAL
                     DALAGPSGPPELILLDLNMPEMDGIEFVRHLVERHYAGSLVLISGEDERMLQASTKLV
                     QAHKIKVLGHLRKPVSPTALAALLEGWAPAATARLRTAKAAYDVEAVRAAINNGELVN
                     YYQPKVAVADGRVVGVETLVRWRHPVDGMVFPDQFIGVAEAHGLIDDLTRVVLRAALA
                     QARVWRDAGLALHVAINVSMDNLAALGFADFVAAEALAAGVSPTEIVLEVTESRLMAD
                     LRVPLEILTRLRLKRFRLSIDDFGTGHSSLAQLRDIPFDELKIDQGFVHRAWHNETLR
                     AIFDASLGLARQLNMEAIAEGVEDQADWDFLRSTRCDLAQGYFIARPMPAAELPGWIA
                     SWQARRRDLVPAPAEGWTE"
     gene            complement(2727..8750)
                     /locus_tag="PLL14_00235"
     CDS             complement(2727..8750)
                     /locus_tag="PLL14_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG02372.1"
                     /translation="MALAYLLLTLPVLLVNAAQGYALPLWPAAGLALAALAAGGWRLW
                     PGVWLGALVAECLLRLQLGNTLAWPELLAQSALIAGGDALQAVLGALLVRRVFANAEP
                     LATASSTLRFLVLGGPVSCLIAATVGVAALRLIGGLPGPELPGKWLTWWVGDSLGVLL
                     FAQLVLLVLPETRRHWRGRVLQISVPILVTGVLLLGGYGLVSRVEQAAAQKNFKVAAE
                     ELYDRVMVRMSKKIGAIEGTAALFEASDHVSRAEFAHYAARIMPQTDTQALAWLPRVA
                     LAERAAVESTARREDIADFVFRERGAQGLLVPAAERAEYYPALYVAKLGGKTAVLGAD
                     AGSREINRAALQSATDTTLPALSEPDGFYDQDGVRRDDWLLYLAVYRAGFDARSTGID
                     ARREALRGFVVLAFSAQQLLADVGNSAQHNLAYRLSTVSTSAPPGLILEHAVPQQRIN
                     RPDATYQLPGLAGERLQLEIWDRVPWQAGHSETEHFYLLGGVLLALLSVMFILTAAGQ
                     SVRVAREVARRTEELRASEAYNRSIVDSNPDCLKLISLDSRLLYMAPHACRMMEVDDF
                     CQIENADWLAFWQGDARMAAEVAVDAARNGAMGRFQGFCPTLKGNPRWWEVTISPILG
                     ADGQPARLLAVSRDITAQRALDLAVRESEARLRSIVDNTAAFVGEMTPDGVLVEANRM
                     ALTIGGLQRKNVVGKRLEDTWWFAHSPEVQAQIRDDIAKALRGEVVRHDVDVRAADGT
                     TFPMDFMLVPVANAEGRIVKLIPSGVDISERKRAEADVLRLNRNLEAQVLARTESLRR
                     TEREFRSTFEGAGLGIAHISPEGRFLRVNPQLCAYSGYAEDELLGQKISLLPHPDELA
                     AGLQAFAPVLRGEAAVMTIESRMQCKDSSPLWIHLTASLVRDEAGEIEYIVCFIKNIS
                     ERKRLDAEFMAQQTVLRHILENLEEGVVCCDPAGNLTFFNKATQQWHGTDIRNVPMED
                     WARFYALFQGDGKTLFKTEELPIIRALSGEVVRNVEMVIVAQGQPPRFLVGSADPMFD
                     DDGRKLGAVLVMHDVTQRRHAETALRKTNEELEQRVAARTAALSEVSERIAAILATVV
                     DGIITIDARGTIETFNPAAERLFGYAAAEAIGQNVKLLMPEPYHGEHDGYLAHYHDTG
                     EARIIGIGREVVGRRKDGSVFPMELAVSEMRLGPERQQIGFTGIVRDITQRKAGERAA
                     RYLRDAVQAIPEAFAIYDTDDRLLLCNQSYLAFYHLGASEDAIGRSFEDIVRFGLARG
                     DYPNAIGREEAWLAERLEAHRNPRPAFDQSMADGCWVRISESRMSDGSTAGLRTDITA
                     LKQAEAAADAANRAKSDFLATMSHEIRTPMNGVIGMIDVLQQTSLHGYQVEMVETIRD
                     SAYALLGVIEDILDFSKIEAGKLDVENVPIPIATVVEKACGMLDHLAVDKQVDLTLFT
                     DPALPALVLGDAQRLRQIIINLANNAIKFSAGREQPGSVSVRAVLAGQRPDGIVVDIR
                     VTDNGIGMDAEMLGRLFSAFTQADASTTRRFGGTGLGLSIARNLAGLMGGKIDVQSAP
                     GEGSIFTLQLPCVPVADHEALDAHPATRGDIASAVDGLCCRVVGNADSLADDWAAYLT
                     AAGAVVERAADLAAARELTAPQTPGPCLWLIDTGHRAPDTNELHALAAARTADDLRIL
                     VVGRGQRRRLRRLDVAGRLWDIDANVLTRQGLLKNVAIAAGRLAADEQAPTIGKSAAA
                     LAPPPRADALRQGRLILVAEDNETNQKVIVQQLALLGFAADVASDGEQALARWQSGNY
                     ALLLSDLHMPKMDGYELTAAIRGAERAAEAENPGGARRIPIIALTANALRGEAVRCLD
                     AGMDDYQSKPTPLAELKAMLEKWLPAVVTVAADSSASIAVPAAPTVLAVNVSVLAALV
                     GDDPATIREFLTDFRASANTIAAELATACAAGDAAEAAALAHKLKSSARSVGALALGE
                     LCAEMEAAGQIGQSSALTGLWPRFAMEMATVDAALADLTARDGKTGSES"
     gene            complement(9364..11637)
                     /locus_tag="PLL14_00240"
     CDS             complement(9364..11637)
                     /locus_tag="PLL14_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG02373.1"
                     /translation="MNARLTPPMVNVSLRITSIRSRGKAGGAVFAGVTDGAEHYAVVS
                     DYRLLPDASLIEKGQVWLIEGPVTMRSATVGSTAILRHEQVINATAAEMLRPTGRNMI
                     DWIANCPDCAGIGQVKARRLYDRFGPDLADLIEDRDLEALTQIISAEAAELLCNAFDK
                     FKVANALLWLDRVGMSRKMGQSVVSYWKGEAQEKIEANPYALISFEADWKKVDTFVRT
                     RLGIAEDDPRRLAGAVEDVLYGSMRHGHTCLPESEARTRLSRLLDSAELAERALNPLP
                     QNDPQSEDVSGIPFHRIDGLLQAHGAWLIESYVARRLHEMVAGEDESGQSGLFAQIAP
                     HPRGIEQVIDDYEATHGITLSEEQRLAVRTSAANHLSLILGGAGTGKTTVLKALYEAL
                     GDQRENMPIYQLALAGRAAQRMAEATQREAMTIAGFLAGIEPGSIEIGSAVVVDEVSM
                     VDVILMYRLLRHIPSGVRLILVGDPSQLPPIGPGLVLHALAELPSIPQTELKVVKRQS
                     SASGIPQVAAAIRAHQVPAWVEYQGKGSGVSFVPCDDARLEDTVQAIYEDLGGNGTDY
                     AVQILSITNANLGGVKNLNVALHNRYQADGEVVMALDAEYGNVAANTLDRIPLCVGDL
                     VIFTENNYKLGLRNGSLGRIVGALPVSEPDDPCCRCMFEGGEYLLDSTQVQTLSHSYS
                     ITVHKSQGSQFTRVIVPIRKSRLLDQTLIYTAVTRGVDQVVLVGDLDATNASIKAPAT
                     AARRNVMLPRLLTKGLK"
     gene            complement(11634..12104)
                     /locus_tag="PLL14_00245"
     CDS             complement(11634..12104)
                     /locus_tag="PLL14_00245"
                     /inference="COORDINATES: protein motif:HMM:NF040697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPA1267 family protein"
                     /protein_id="HOG02374.1"
                     /translation="MSQSNQSGQQLARDNLAKFQSWIAEREVTNDWHDYLRGDKLNRS
                     EIAAECDFSLSVMRQNPAVKEALEALESRLTTLGLTQPLRTAPDASNEAAAASEKVID
                     KRIMAAKGRAEARVKALEEQNASLKAEVASLRERLRRFEHLDDHLSRTGRLLPA"
     gene            complement(12101..12814)
                     /gene="gmtX"
                     /locus_tag="PLL14_00250"
     CDS             complement(12101..12814)
                     /gene="gmtX"
                     /locus_tag="PLL14_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-mobile-trio protein GmtX"
                     /protein_id="HOG02375.1"
                     /translation="MTTDVHPDEVLKAILDKGNRRDKEEKLRKIHELCSLEYSRHSQG
                     ARDLSIANMARVAESHGLFKARTIYNKQSEDYAALIKAWEAYNGPKPSKAVKAQLAPA
                     DKHAFLKKIEDPAVRSLCQMAIIERDKIRAELNMLKSVTVVPIDMRPLGAEIAKGSTN
                     VAIIEPAAQLTDSERNALAAAIDPKSLAQQKLRIGDTGEIVDERGRFVFNPGFATAIA
                     KILGRSDRTPMLEVKGKRV"
     gene            complement(12792..16040)
                     /locus_tag="PLL14_00255"
     CDS             complement(12792..16040)
                     /locus_tag="PLL14_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPA1269 family protein"
                     /protein_id="HOG02376.1"
                     /translation="MKSKLTQAQKDEIQRRAADGEIRTVIAKTMGIAVHLVYDRAPKV
                     PPRVTREQKMEIVRRVESGETKNALAKEFGLSKVTVQDYTKHVIRKLPEVPQEVRKEI
                     IRRAIEGESAKTIARELGYSVGTVDYHIKNVASDYQLSDEQTAAIWHARQTGKKYGQI
                     AEQLKLPRRVIYAALGIYRKKSTDDALRAQAIRAMETGESSTSVATRLGVSSTAVLSW
                     FRRDVAEGKAKRPENVSKSDDDQFMWIAKRDSSLEHWRKLIVEYAATHSPPFSTFINA
                     LSVFVDRYLLAHRLPLNPADLLNRKQLVPDFYAACPQGKSGRTYNTQIYNLIEWVLDS
                     EQFADMEDGEPLRFTDLYRNPIDPNAGGEDALPQARESTKTLLSYLLVSLLRKQIVQG
                     PNFRDWTWAQGLMGNKDLDGNSLGPDWFEVSEDRLDKSDLDCVWRVRSPSTVDRPVLE
                     MWSPVRWVHALLHLQTPVRGGQARMADSGEADTFIWHDAKFIPNPSKLCQGSARNPRQ
                     QGVFRRPSTEEESAGAKITLYFNSNKTADIGKGGSALGFECPWPQMEESDEDPYRWLA
                     KLRDWQMKYNPIQRLTRWIDLEGATKLSPQSKNRLQDYPDTAFLFRLPEDAKHPDWPL
                     SSNRANVAWQILLTAFESALGEQGIRSPTGERIELINPETGRAWSSPHATRPSLITHL
                     IMDGNVPPIIMTKVVGHARFIMTLYYTKAGLVGIQNSIKQATKVLEETKHKTFERDLL
                     SANAERMREIAVFNTEDWTTVLPLNSADRSPLGWYYMHDRVCFAGGNTHGDNQVPGCH
                     NGGPVRTKEGRNQKDIHDPVPGGVRNCCRCRWSASGKEHLDSLAATLTNRSWHLQNAS
                     NEALATLRERDAILKSKARTEAANEPFGQTHDLTSAERRHEAAMHRMQELTLDVAALN
                     RTIDRVMALPVNVDGPTVLALQGDALTAHAVIEDASELLQLANVVGDMELYPDLQSNT
                     GEAVYAYADLLDNAFECEGHPRILVRLSKDEKMQCANALMRQLERHANPQNHLLGRRA
                     VVEIIDRGESLQKLLGVKLNEIARQALPAKNQTPLRLVKPTTEDDHDDRRAS"
     gene            complement(16050..17435)
                     /gene="gmtY"
                     /locus_tag="PLL14_00260"
     CDS             complement(16050..17435)
                     /gene="gmtY"
                     /locus_tag="PLL14_00260"
                     /inference="COORDINATES: protein motif:HMM:NF040693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-mobile-trio recombinase GmtY"
                     /protein_id="HOG02377.1"
                     /translation="MVFSVVSGKVYSDNTGTSLQIPVLMTPQGPVRPLIDYCLSLRRS
                     LAWQEKLVRGVKLFLEYLEVNAVQGEEEWRMFRNFSNALHVGTVDPDTRDDPSNLWWS
                     GMAPREANFMITQLSDFFDWLGREDAPRAAKFNPRYEGNTHDRRLDYQAYKYRRDKAF
                     LGHAWSTKPQERGRLTRGERTPKVLPKRPPAFPEERFEELLVKGFRVAGKQDYRGMLI
                     TLLLFGGGLRVSEPFHLYVADVHPHWDDPSRAFVAVHHPSLGYAPDDWKTPTGQRGSR
                     QEYLALKYGLVPRHKIRGKLNAGWKHPALDDRWYMHVHWYPETYGLWFMQIWKCYMAQ
                     IADIERNHPYAWINVGSDKQGGIYTIAQYEKALQNAVGRIGLIYGKSYGTTAHGGRHA
                     YGQRTRRGGIDPIIVQRLMHHCSPDSQIVYTQAEFNEAQDTLRKGVETLRQNNLMVPS
                     QQLALTSELDN"
     gene            17581..>17817
                     /locus_tag="PLL14_00265"
     CDS             17581..>17817
                     /locus_tag="PLL14_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuC"
                     /protein_id="HOG02378.1"
                     /translation="MGVINGCVVARSAEVKALGIPMGVPWFQLQAQARKHGIVACSSN
                     YALYGDMSNRVVEVLSSFSPKIEVYSIDESFLDFT"
BASE COUNT         3302 a         5476 c         5397 g         3642 t
ORIGIN      
        1 aagcggcggc tgtaccctga aaactgctga aaggccaatt cacactcctc ggcctgaccg
       61 gccatccagg acttgctgct tggacgactg caccttggcg cattgccgcc gttcgagggg
      121 ggcgccgaaa aaacctgggc cgacgagtcc gggtgaccgc ttaccaaaaa acgctactca
      181 cactttcgac ccacacccgt cacaagataa atgcatagtc attgaccgct tcagctccca
      241 ttgctgtctc ttgcagtcgc atcaataaac cggatcagat ccggtaatcg ccgaaggctc
      301 atgcccactg cgccggcgtt cacgccccgt tcagcacctg cctcaacttg gcgatcagcg
      361 tgtcgcgcgc aaagggtttg accaggaatc cgcacgcacc tgccttcagg ctgtcgacca
      421 cgacacttcc ctcgctctgg cccgtgatca tgatgaccgg tagcttggcg aattgcggca
      481 cggccttgag ccgccgcgtg gtttcgatgc cgtccatttc gggcatcttg aaatccataa
      541 ggatgacatc gggccgcgtc ttgcgcatca ccgcgagcgc ctcaacgcct ttggcggcaa
      601 agatcagctg atagttctcc gctgcgagaa tcttcgctac cagcttctgt tgcagctcgt
      661 cgtcatcgac caccagcacg gtgggtcgga cgctatcggc cagcacattc agctcgcgga
      721 ccgacgccat atgcgtcgag aactcctgct tgaaatcgtc ggcccactgc ttgagcggcg
      781 ccaccgattt ggctgcggta cggaaacgtt ccatgacatc ctcgcgtttg aggcggccga
      841 tttcatcctg cagggcaccg gcattattga ccgtgaccgc accgggcagc gcgcctgcga
      901 tcaggcgccg ggagaacccg tccagcgccg cgccgacatc ctgttccgcc tgctgcatgg
      961 cgcggctggc aacatcgatg tgccggttgc ccgtcgccat ctgcgcgtcg agctgctttt
     1021 ccagttccgc caggcggcgt gcctgtgcgg caaactccgc caccgacggc ccgccgccct
     1081 tgatcacaga gagttcgtgc agcgcacgat gaatcgacat gggcagccgc gacccgtcat
     1141 agttcatcgg ccagaacagc acgtagtcgt cgaaataact cttcttgcac agctcgtaga
     1201 cccgcttcac ttcatccttg ttgcacagga tcacggtgcg gtggaggtgc tgctgaaccg
     1261 ccgggtacag gcgatataac ccgaggtaat agcgctcggc cttctccagc gtggcaaagg
     1321 ccaacaccag cacgtcgggg ttgcggcgct cgaaatcctg cgcgatcagg tcgggattgg
     1381 tcgagacaaa gacattcttg aattcctccg cgagattctt cctgaccatc tcggcgtcgg
     1441 tgacatcgtc ggtggcgatc aggatggcgg cactgatcag gtcggttgcg gccattattc
     1501 agtccatcct tccgccgggg ctggaaccag gtcgcgccgc cgcgcctgcc agctcgcgat
     1561 ccagcccggc agctcggccg ccggcatcgg ccgggcgatg aaataaccct gcgccagatc
     1621 gcagcgcgtg ctgcgcagaa aatcccagtc ggcctggtct tccacccctt cggcaatggc
     1681 ttccatgttc agttgcctgg ccaggccgag gctggcgtca aagatcgccc gcaaggtttc
     1741 gttgtgccag gcgcgatgca cgaagccctg atcgattttc agttcgtcga agggaatgtc
     1801 gcgcagctgg gccaatgaag aatggccggt gccaaaatcg tcgatcgaca ggcggaagcg
     1861 cttcaggcgc agccgggtga ggatttccag cggcacccgc aagtcggcca tcagccggct
     1921 ttccgtcacc tccagcacaa tctccgtggg cgacacgccg gccgcgagcg cctcggcggc
     1981 gacgaaatcg gcaaaaccca gcgccgccag attgtccatc gacacattga tcgccacgtg
     2041 caaggccagc cccgcgtcgc gccagacccg ggcctgcgcc agcgcagcgc gcagcacgac
     2101 gcgggtcagg tcgtcgatca ggccgtgcgc ctcggccacg ccgatgaact ggtcggggaa
     2161 caccatgcca tcgaccggat gccgccagcg caccagcgtc tcgaccccga ccacgcggcc
     2221 gtcggccacc gccaccttcg gctggtaata attgaccagc tcgccattat tgatggcggc
     2281 gcgcactgcc tctacgtcgt acgccgcctt ggctgtccgc aagcgggcgg tcgcggctgg
     2341 cgcccacccc tcaagcagtg cggccagcgc cgtcggcgaa accggcttgc gcaggtggcc
     2401 gagcaccttg atcttgtgcg cctgcaccag cttcgtcgag gcttgcagca tgcgttcgtc
     2461 ctcgccgctg atcagcacca ggctgccggc gtagtgccgt tcgaccaggt ggcggacgaa
     2521 ttcgatgccg tccatttccg gcatgttgag gtcgagcagg atcagctccg gcgggccgga
     2581 tggaccggcg agcgcatcga gtgcagcggc gccgctggtg tagggcgaca gcgccgtaaa
     2641 gccgaggttt tccagctggc gcccgatcag cttgagcatg aaaggttcgt cgtcgagcag
     2701 catgattttg actgcgaaat tgttggtcat gattcgcttc ctgtcttccc gtcgcgcgcc
     2761 gtcaagtccg ccagtgcggc atcgacagtc gccatttcca tcgcaaagcg cggccacaaa
     2821 ccggtcagcg cggagctctg cccgatttgc ccggctgctt ccatctcagc acacaactcg
     2881 cccagcgcca gcgctcccac cgagcgcgcc gaggacttga gcttgtgcgc cagcgcggcc
     2941 gcctctgccg cgtcgccggc ggcgcaggca gtcgccagtt ctgccgcgat ggtgttggca
     3001 ctggcgcgga aatccgtcag gaattcgcgg atcgtcgccg gatcgtcgcc gaccagtgcg
     3061 gcgagcacgc tcacattaac cgccaggaca gtcggcgccg ccggcacggc gattgaagcc
     3121 gatgaatctg ccgctacggt aaccacggcc ggcagccatt tctccagcat cgccttcagt
     3181 tcggccagcg gtgtcggctt cgactgatag tcatccatgc cggcgtcgag gcaacgtacg
     3241 gcttcgccgc gcagggcatt ggcggtgagg gcgatgatcg ggatgcggcg cgcgccgccg
     3301 gggttctcgg cctcggcggc gcgttctgca ccccgaattg cagcggtcag ttcgtaaccg
     3361 tccatcttcg gcatgtgcag atcgctcagc aacaacgcgt agttgccgct ctgccagcgc
     3421 gccagcgcct gctcgccgtc gctggcaaca tcggccgcaa agccgagcaa ggcaagctgc
     3481 tggacgatca ccttctggtt agtttcattg tcttcggcca ccaggatcag ccggccctgc
     3541 cgcagcgcgt cggcacgggg gggcggcgcc agggcggcgg cgcttttgcc gatggttggc
     3601 gcttgctcgt ccgcagccag gcgtccagcg gcaatggcca cgttcttcag cagcccttgc
     3661 cgcgtcagca cgttggcatc gatgtcccac agtcggcccg cgacatccag ccggcgcaga
     3721 cgccggcgct ggccgcgccc gacgacaagg atgcgcagat cgtccgcagt ccgagcggcg
     3781 gcgagggcgt gcaattcgtt ggtatccggc gcgcggtggc ccgtgtcgat cagccacagg
     3841 cacggccccg gcgtttgggg tgcggtcaat tcgcgggccg ctgcaaggtc tgccgcccgc
     3901 tcgaccacgg cgccggcggc ggttagataa gccgcccaat cgtcggccag gctgtcggca
     3961 ttgccgacca cccggcagca caagccatcc accgccgacg ctatatcgcc gcgggttgcc
     4021 gggtgtgcgt caagcgcttc atggtcggcc accggcacgc agggaagctg cagggtgaag
     4081 atcgaccctt cgcccggcgc actctgcacg tcgatcttgc cccccatcaa gccggccagg
     4141 ttgcgggcga tggagagccc caggccggtg ccgccgaagc gccgggtggt cgaggcgtcg
     4201 gcctgggtga aggcgctaaa caaccggccc agcatctccg cgtccatgcc gatgccgttg
     4261 tcggtgacgc gaatgtccac gacgatcccg tccggccgct gcccggcaag caccgcgcgc
     4321 accgacacgc tgcccggctg ttcgcgacca gcggagaact tgatcgcatt gttggccagg
     4381 ttgatgatga tctggcgcag ccgctgtgca tcgccaagga ccagcgccgg caacgccgga
     4441 tcggtgaaca gcgtcagatc gacctgcttg tcgaccgcca ggtggtcgag catgccgcaa
     4501 gccttttcga cgactgtggc aatggggatc ggcacgtttt caacatcgag cttgccggcc
     4561 tcgatcttcg agaagtcgag gatgtcctcg atgacgccga gcagcgcata ggccgaatcg
     4621 cggatggtct cgaccatctc cacctggtag ccatggaggc tggtctgctg cagcacgtcg
     4681 atcatgccga tgacgccatt catcggcgtg cgaatctcgt ggctcatcgt cgccagaaaa
     4741 tccgacttgg cgcggttggc ggcgtcggca gcggcctcgg cttgcttgag ggcggtgatg
     4801 tcggtgcgca gcccggcggt cgagccgtcg gacatccggg attccgaaat gcgcacccag
     4861 catccatcgg ccatgctctg atcgaacgcc ggacgggggt tgcggtgcgc ctctaggcgc
     4921 tcggccagcc aggcttcttc tcggccgatg gcattgggat aatcgccgcg cgccaggccg
     4981 aagcgcacaa tatcctcgaa gctccgacca atggcatcct cagacgcacc aagatggtag
     5041 aaagcgagat acgactggtt gcacagcagc aggcggtcgt cggtgtcgta gatggcaaaa
     5101 gcctcgggaa tcgcctgcac cgcgtcccta agatagcgtg cggcgcgttc gccggctttg
     5161 cgctgggtga tgtcgcgcac gatgccggta aagccgatct gctggcgctc tggacccagc
     5221 cgcatttcgc tcaccgccaa ttccatcgga aagacgctgc cgtccttgcg ccggcctacg
     5281 acttcgcggc cgatgccgat gatgcgcgct tcgccggtat cgtggtagtg cgccaggtag
     5341 ccgtcgtgct cgccgtgata gggctcgggc atcagcagct tgacgttctg cccgatggcc
     5401 tcggcggcgg cgtagccaaa caggcgctcg gcggccgggt tgaaggtttc gatggtgccc
     5461 cgcgcgtcga tggtgatgat gccgtcgacc acggtggcga gaatcgccgc gatccgctcg
     5521 ctaacctcgc tcaacgcggc agtgcgcgcc gccacccgct gctccagttc ttcgtttgtt
     5581 tttcgcagcg ctgtttcagc atgccgtcgt tgggtgacgt catgcatcac caagaccgcg
     5641 ccgagtttgc gtccgtcatc atcaaacatc ggatccgcgc tgccgacaag gaaacgcggt
     5701 ggctgtccct gcgcgacaat gaccatctcg acattccgga cgacctcgcc agacagggcg
     5761 cgaattatcg gtaactcttc ggttttaaaa agggtcttgc catcaccttg gaagagagcg
     5821 tagaacctcg cccagtcctc catggggaca ttgcgtatat cggtaccgtg ccactgttgg
     5881 gtcgccttgt tgaagaaagt aagattgccg gcagggtcgc agcaaacaac gccttcctcg
     5941 agattttcga ggatgtgacg caacacggtc tgctgcgcca tgaattccgc gtccagccgc
     6001 ttgcgctcgg aaatattctt gatgaaacag acgatgtatt cgatctcgcc cgcttcatcg
     6061 cgcaccagcg aagcggtcag gtggatccag agcggcgagc tatccttgca ctgcatgcgg
     6121 ctttctatcg tcatgaccgc cgcttcaccg cgcaagacgg gagcgaatgc ctggagcccc
     6181 gcagcaagtt cgtcgggatg cggcaacaag gagattttct gcccgagcaa ctcgtcttcg
     6241 gcataaccgg agtacgcaca tagttgcggg ttaacccgca ggaaacgccc ttccggactg
     6301 atgtgtgcga tacccagacc agcgccctcg aaggtcgaac gaaattcccg ctcggtgcgc
     6361 cgcagcgatt cggtccgtgc caaaacttgc gcttcgaggt tccggttcag cctgagaaca
     6421 tcggcttcag cgcgcttgcg ctcgctgata tcgacgcccg acggaatcag cttgacgatg
     6481 cggccctcgg cattagcaac cggcaccagc atgaagtcca tcgggaaggt cgtgccgtcc
     6541 gccgcgcgaa catcaacatc gtggcgaacc acctctccgc gcaaggcctt cgcgatgtcg
     6601 tcacggatct gcgcctgaac ctcgggcgaa tgggcaaacc accaagtatc ctcaagccgc
     6661 ttgccgacga cgtttttacg ctgcaagcca ccgatggtca gcgccatgcg attcgcctca
     6721 accaacacgc cgtcaggggt catttcaccg acgaaggccg ccgtgttgtc gacaatgctg
     6781 cgcagccgcg cttcactttc gcgcacggca aggtcgagtg cccgctgcgc ggtgatgtcg
     6841 cgcgaaaccg cgagcagccg cgccggctgc ccgtcggcgc cgagaatcgg actgatggtg
     6901 acctcccacc agcgcggatt gcccttcagc gtcgggcaaa acccctgaaa cctgcccata
     6961 gcgccgttgc gggcggcatc taccgcaact tcggcggcca tccgggcgtc gccctgccag
     7021 aaagcgagcc agtcggcatt ctcgatctgg cagaaatcgt ccacctccat catgcggcag
     7081 gcgtgcggcg ccatatagag cagccgggaa tcgaggctga tcagtttcag gcagtccggg
     7141 ttgctgtcga cgatactgcg gttgtaggcc tcgctggcgc gcaattcttc ggtgcgccgg
     7201 gccacttcgc gggcgacgcg aacgctctgc ccggcagcgg tcagaatgaa catcacgctg
     7261 agcagggcga gcagcacacc gcccagcaga tagaagtgtt ccgtctctga gtgtcctgcc
     7321 tgccatggca cgcgatccca gatttcgagc tgcaagcgtt cgccagccag tccgggaagc
     7381 tggtacgttg catctggacg gttgattcgt tgttgcggga cagcgtgctc gaggatcaga
     7441 ccaggggggg ccgaagtgga cactgtcgac agccgatagg cgagattgtg ttgcgcgctg
     7501 ttacccacat cggccagtaa ctgctgcgcg gagaacgcga ggacgacaaa accgcgcagg
     7561 gcttcacggc gggcgtcgat gcctgtcgag cgggcgtcga aaccggcgcg gtaaaccgca
     7621 aggtagagca gccagtcgtc cctgcgcacg ccgtcctgat cataaaaccc atcaggctcg
     7681 gacagcgccg gcagggtcgt atcggtggcg ctttgcaggg cggcacggtt gatttcgcgg
     7741 gatccggcat cggcgcctag cacagcggtt ttccccccca atttggcgac ataaagcgct
     7801 gggtagtatt cggcacgctc ggcggcaggc accagcagcc cctgtgctcc gcgttcacgg
     7861 aaaacaaaat cggcgatgtc ctcgcgtcgc gccgtgctct cgacagcggc gcgctcggcc
     7921 agcgccaccc gtggcagcca ggccagcgcc tgtgtatctg tctgcggcat gatgcgcgcc
     7981 gcatagtggg caaattcggc gcgcgacaca tggtcgctgg cttcgaacag ggcggccgta
     8041 ccttcgatcg cgcctatctt cttcgacatg cggaccatga cgcggtcgta caactcctcc
     8101 gcagcgactt tgaaattctt ttgcgcagca gcctgctcga cccggctgac caagccatag
     8161 ccgccgagca gcagcacgcc agtgaccagg atcgggaccg agatctgcag tacgcggccg
     8221 cgccagtgcc ggcgcgtctc tggcaacacc agcaggacca gttgcgcgaa gaggaggaca
     8281 ccgaggctgt cgccgaccca ccaggtcagc catttgccgg gcagttccgg gccgggcagg
     8341 ccgccgatca ggcgcaacgc agcgacgccg acagtggccg cgatcaggca ggaaaccggc
     8401 ccgcccagta ccaggaaacg cagcgtgctg gaggccgtcg ccaacggctc ggcgttggcg
     8461 aaaacgcgac gcaccagcag tgcgcccaac accgcctgca gggcgtcgcc cccggcgatc
     8521 agcgcggatt gcgccagcag ttctggccat gcgagggtgt tgccaagctg caagcgcagc
     8581 aggcattcgg caaccagggc gcccagccag acccccggcc acaggcgcca gcccccggcg
     8641 gcaagcgcgg cgagcgccag cccggccgcc ggccacagcg gcagcgcgta gccctgcgcc
     8701 gcgttgacca gcagcaccgg cagggtgagc aaaagatagg cgagcgccag accagccagc
     8761 cgcgccggca gcgatctggc cgctaccgca ggatcagcgg gggggggggc atccctagct
     8821 gacgggcatc ttcgggaggc gtgctgattg aaactttcat gatatttccg tggtgtaggt
     8881 aatgcttact gcaatcaatg gcgcggggct gcacaaaagc cataacgcgg tcacaagttt
     8941 cagcctaccc cttaacggga cagtaccaac agcataaatt gtacagcggc gtagcgggcc
     9001 tggcgggaaa gctggccaag ttcttcaaca ttagtccagc ggtattcgtg cccggctgaa
     9061 gcaaggtctg cgctcttccg ggtcggtatc gcgtacagca ggtgccggcc ggttacctgt
     9121 cgttcaaaat tgggtcgttt tcccgtctcc accgaccgca gatggccgta cccggacctt
     9181 ggggcgctgc cccaaacccc gcactcgccg tggggcagcc ggggcgggtt aaaaaccaac
     9241 ccaccccagc agccgggagc gttgggtcgc gtcgttcagt caagggcttt cgcggcataa
     9301 acaggcttcc tcccgcaagc gggcccctcc tatttattcc acggtccctt gacagcccgt
     9361 cgctcatttc agtcccttgg tcagcaatct cggcaacatc acattccgac gagcggcggt
     9421 ggctggggct ttgatggagg cgttcgtagc atccagatct cccaccagca ccacctgatc
     9481 gacgccccgg gtgactgctg tgtagatcag ggtttggtca agcaagcgag acttgcggat
     9541 cggcacgatc acccgcgtga actggctccc ctgactcttg tgcaccgtga tgctgtagct
     9601 gtggctgaga gtctggacct gggtggagtc aagcaggtac tcaccgcctt caaacatgca
     9661 gcggcagcag gggtcatccg gttcgctgac cggcaacgct ccgacaatgc gcccgagact
     9721 gccattgcgc aggccaagct tgtagttgtt ttcggtgaag atgactaaat cgcccacgca
     9781 cagaggtatg cggtcgagcg tattggcggc gacgttgccg tactcggcgt ccagcgccat
     9841 gaccacctcg ccatcggcct gatagcgatt gtgcagggcg acattgaggt tcttcacccc
     9901 gcccaggttg gcgttggtga tggagaggat ttgcacggcg tagtcagtgc cattgccgcc
     9961 caagtcttcg taaatggcct gtaccgtatc ctcaagtcgc gcgtcatcgc agggcacgaa
    10021 ggacacccct gatcccttgc cctggtactc cacccaggcc ggcacctgat gggcgcggat
    10081 cgcggcggca acttgcggga tgccactggc gctgctctgg cgcttgacca ccttcagttc
    10141 ggtttgcggg atgctcggca gctccgccaa ggcatgcagg acgagtccgg ggccgatggg
    10201 cggaagctgg ctggggtcgc cgacgagaat caggcgcaca cccgagggga tatggcgcaa
    10261 caaccgatac atcaggatca catcgaccat cgagacttca tcgacgacga ctgcgctacc
    10321 gatttcgatg gaacccggct cgatcccggc aagaaagccg gcgatggtca tggcctcgcg
    10381 ttgggtggcc tccgccatgc gctgggcggc acggccggcc aaggcaagct gatagatcgg
    10441 catattctca cgctgatccc ccagtgcttc atacagcgcc ttgagcacgg tggtcttgcc
    10501 ggtgccggct ccacccaaga tcaggctcag atgattggcg gccgaggtac gcacagcgag
    10561 gcgctgttcc tcggacaggg tgattccgtg cgtggcctca tagtcgtcaa tgacttgttc
    10621 aatgcccctg gggtgggggg cgatctgcgc gaagaggccg gactgtcctg attcatcctc
    10681 gcccgcgacc atttcgtgca ggcgacgggc aacgtagctt tcgatcaacc atgcgccatg
    10741 ggcttgcagg aggccgtcaa tgcggtgaaa tggaatgcca gatacatcct ccgattgggg
    10801 atcattttgt ggcaaaggat tcagcgcccg ctcggccagc tcagcgctgt ccagaagccg
    10861 ggacaggcgt gtgcgggcct cgctctcggg gaggcaggtg tggccgtgcc tcatgctgcc
    10921 gtacagcacg tcctcgaccg ctccggcgag gcggcgggga tcgtcttcgg cgatgccgag
    10981 gcgggtgcgg acaaaggtat cgactttctt ccagtcggcc tcgaaggaga tcagggcata
    11041 ggggttggcc tcgatcttct cctgcgcctc gcccttccag tagctgacga cgctctggcc
    11101 catcttgcgg ctcatgccca cgcgatccag ccagagcagg gcattggcaa ccttgaactt
    11161 gtcgaaggcg ttgcatagca gctcagcggc ttccgcgctg atgatctggg tcagggcctc
    11221 caggtcgcgg tcttcaatga ggtcagccag atcgggaccg aagcggtcat acagccgtct
    11281 ggccttgacc tgcccgatcc cggcacagtc ggggcagttg gcgatccaat cgatcatgtt
    11341 gcgcccggtg ggtcgcagca tctcggcggc ggtggcgttg atgacctgct catgccggag
    11401 gatggcggta ctgccgacgg tcgcgctgcg catcgtcacc ggcccctcga tgagccaaac
    11461 ctgacccttc tctatcaggc tggcatccgg aaggaggcgg tagtcggaga cgacggcgta
    11521 gtgctctgcg ccatcggtca cgccggcgaa cacggcacca ccggccttgc cgcgactgcg
    11581 gatcgacgtg atgcgcaggc tgacattcac catcggcggg gtgagccggg cgttcatgcg
    11641 ggcagcagcc ttccggtacg agaaaggtga tcatccagat gctcgaagcg gcgcaaccgc
    11701 tcacgcaagc tggcgacttc ggccttgagg ctggcgttct gttcttccag cgccttgacc
    11761 cgcgcttccg cgcgaccctt ggcggccatg atccgcttgt cgatgacttt ctcggaggcg
    11821 gctgcggcct cattggaagc gtcaggagcg gttcttaggg gttgggtgag gccaagggtt
    11881 gtcaatcgac tctccagggc ctccagagcc tccttgacgg ccgggttctg acgcatgacc
    11941 gacagggaga agtcgcattc ggcggcaatc tcgctgcggt tgagcttgtc gcctcgcagg
    12001 tagtcgtgcc agtcgttggt gacctcgcgc tcagcgatcc acgattggaa cttggcgagg
    12061 ttgtcacgtg cgagttgctg gccggattgg ttggattggc tcatacccgc tttcccttca
    12121 cttccagcat tggcgttctg tcactcctgc cgaggatttt ggcgatggcg gtggcgaagc
    12181 ccggattgaa gacgaagcgc ccgcgttcat ctacgatctc cccggtatcg ccgatacgca
    12241 gcttttgctg agccagggac ttgggatcga tggcggcagc gagagcattg cgttcggaat
    12301 cggtgagttg cgctgccggt tcgattatcg ctacattcgt tgatccctta gcaatctcag
    12361 cccccaacgg cctcatgtcg atgggaacga ctgtcacgct cttgagcatg ttgagttcgg
    12421 cgcgaatctt gtcgcgctca atgatcgcca tctgacaaag gctacggacg gccgggtctt
    12481 caatcttctt gaggaaggca tgcttgtcag ctggtgcaag ctgggccttg accgctttcg
    12541 acggcttggg accgttgtag gcttcccacg ccttgatcag tgctgcgtag tcttccgact
    12601 gcttgttgta gatcgtgcgc gccttgaaga gtccatggct ctcagcaacg cgggccatgt
    12661 tggctatgct caggtcgcgg gcgccctggc tgtgccggct gtattccaat gagcacagct
    12721 catggatctt gcgcagcttc tcttccttgt cgcgacgatt gcccttgtcg aggatcgcct
    12781 tcaacacttc gtcaggatgc acgtcggtcg tcatgatcgt cctccgtcgt tggcttcaca
    12841 agacgcaagg gcgtctgatt cttggcgggc aatgcctggc gcgcaatctc attcagcttc
    12901 accccaagca gcttttgcag cgactcgcca cggtcaatga tttcaaccac tgcccggcgt
    12961 ccgagcagat ggttttgcgg gttggcgtgc cgctccagtt gccgcatgag ggcgttggcg
    13021 cactgcatct tttcgtcctt ggatagtcgt actaggatgc gtggatgacc ctcgcactcg
    13081 aaagcgttgt cgagcagatc ggcataggca tagaccgcct cgccagtatt cgattgaagg
    13141 tctgggtaca actccatgtc tcccaccaca ttcgccagtt gcagcagctc actggcatct
    13201 tcaatgacag cgtgggcggt cagggcatcg ccttgtaacg ccagtacagt ggggccatcg
    13261 acgttgacgg gcagggccat gacccgatcg atggtgcgat tcagcgctgc aacatccaac
    13321 gtgagttcct gcattcgatg catcgcagct tcatggcggc gctcggcgct cgtgagatca
    13381 tgagtctggc cgaaaggttc attagccgcc tcggttctag ccttggactt gagaatcgca
    13441 tcacgttctc gaagtgtcgc caaggcttcg ttcgaggcat tctgaaggtg ccaggatcgg
    13501 ttggtcaggg tcgcagcgag agagtcaagg tgctccttgc ccgatgcact ccagcggcaa
    13561 cggcagcagt tacgcacgcc gcccggaacc gggtcgtgga tgtctttctg atttcgacct
    13621 tctttggttc tgaccgggcc gccgttatgg caaccgggga cctggttgtc tccatgtgta
    13681 ttgccgcctg cgaaacagac tcgatcatgc atgtaatacc aaccaagtgg cgagcgatcc
    13741 gcagaattca gcggtagaac cgtagtccag tcctctgtat tgaatacggc gatttcacgc
    13801 atccgttctg catttgcgct aagcagatcg cgctcaaagg tcttgtgctt cgtttcttcc
    13861 aatacttttg tcgcctgctt gatcgaattc tggatgccga caagtccggc cttggtgtag
    13921 taaagggtca tgatgaaccg cgcatgaccg acgaccttcg tcataatgat aggcggcacg
    13981 tttccgtcca tgatcaggtg cgtgatcagg cttgggcggg tcgcgtgggg ggaactccat
    14041 gctcgacctg tctcgggatt gatcagttcg atgcgttctc ctgtaggact gcgaatgccc
    14101 tgctcgccga gagcgctttc gaacgcggtt aggagaattt gccaagcaac attggctcta
    14161 tttgatgaaa gtggccaatc ggggtgcttg gcatcttccg gaagccgaaa cagaaaggct
    14221 gtatcgggat agtcctgcaa ccgattcttg ctctggggag agagtttggt agcgccttcc
    14281 aggtcaatcc atcgcgtcag acgttggatc gggttgtact tcatctgcca gtcccgcagt
    14341 ttggcaagcc aacggtaggg gtcttcgtcc gattcctcca tctgcggcca aggacattcg
    14401 aaccccagtg ctgatccgcc tttgcctata tcggcggtct tgttggagtt gaaatagagc
    14461 gtgatctttg ctcctgcgga ctcttcctcg gtcgaggggc gacggaacac accctgctgg
    14521 cgtgggttgc gggccgaacc ttggcagagt ttgcttggat tgggaatgaa tttagcatcg
    14581 tgccagatga aggtgtctgc ctcgccgctg tcggccatgc gcgcctgacc tccccgtacg
    14641 ggggtttgca ggtgcagtaa ggcatgcacc cagcgcacag gcgaccacat ttcgagaacg
    14701 ggccgatcta cagttgaagg gcttcgtacc cgccagacgc aatctagatc gctcttgtcg
    14761 agtcgatcct cgctaacctc gaaccaatcg gggccaaggc tgttcccatc gaggtcttta
    14821 ttccccatca aaccctgcgc ccaagtccaa tcgcggaagt tcggaccctg tacgatctgc
    14881 ttgcgcagaa gagagacgag caagtaactt agcaaggtct ttgtggattc ccgagcttgc
    14941 ggtaatgcat cttcaccacc agcattagga tcaatcggat tacgatagag gtcggtaaag
    15001 cggagcggtt cgccgtcctc catatcggca aattgctcgc tatccagcac ccactcgatc
    15061 aggttgtaaa tctgggtgtt atatgttcgc cctgacttac cctgcggaca ggcagcatag
    15121 aagtcaggta ccaactgttt gcggttgagt aagtcggctg gattcagggg aaggcgatgg
    15181 gcgaggaggt aacgatctac gaatacgctc agagcgttaa tgaaggtaga aaatggggga
    15241 ctgtgggtgg cggcatactc gacaatcaat ttgcgccaat gctcaagact tgagtctctt
    15301 ttggctatcc acatgaactg atcatcgtct gattttgaga cattttccgg tcgcttggcc
    15361 ttaccttcgg caacatcccg cctaaaccaa ctgagtacag cagttgaact aacccccaat
    15421 cgtgtggcta cggaagtgga agattcgccg gtttccatag cgcgtatcgc ctgagcgcga
    15481 agggcgtcat cagtgctctt ctttctgtat atccctaatg ccgcatagat aacccgtcgt
    15541 ggaagtttca gttgctcggc aatctgcccg tatttcttac cagtctggcg cgcatgccat
    15601 attgctgccg tttgctcatc agaaagttgg tagtcgctgg cgacattttt gatgtgatag
    15661 tcaactgttc cgacgctgta gcctaattcg cgagcgatcg tttttgctga ttcaccctca
    15721 attgcgcggc gtatgatttc ctttctgacc tcttgtggca cttcgggcag ttttcgaatg
    15781 acgtgcttgg tgtaatcctg aacggttact ttggataggc cgaactcctt cgcgagagcg
    15841 ttctttgtct cgccactttc gacgcgtctt acgatctcca ttttctgctc acgcgtaaca
    15901 cgtggcggca ctttcggcgc gcggtcatag acaaggtgga cggcgatacc catcgttttt
    15961 gcgataacag tacggatctc accatccgcc gctcgtctct gaatttcgtc cttttgcgcc
    16021 tgcgttagct tgcttttcaa ctttgtccct cagttatcaa gttcgcttgt cagcgctaat
    16081 tgctgcgatg gaaccatcag gttattttga cgcaaggttt ctacgccctt gcgcagcgtg
    16141 tcctgcgcct cattaaattc ggcttgggta tatacaatct gactatctgg cgagcaatgg
    16201 tgcatgagtc gctggacgat gatcggatcg atgccccccc gtcgcgtacg ctgaccgtag
    16261 gcatgccgtc ctccgtgggc cgtggtgccg tagctcttgc catatatcaa cccaatgcgc
    16321 ccgacagcgt tttgcagggc cttttcatac tgcgcgatgg tgtaaatacc cccctgttta
    16381 tcgctaccta cattgatcca tgcataggga tgattgcgct caatgtcggc gatctgcgcc
    16441 atgtagcact tccagatttg catgaaccaa agcccgtagg tttctggata ccagtgcaca
    16501 tgcatatacc aacggtcatc cagcgcagga tgcttccagc ccgcgttgag cttgccccgt
    16561 atcttgtgac gcggaaccaa gccatattta agtgcaaggt attcctgtct tgaaccccgc
    16621 tgaccggttg gggttttcca gtcatcgggc gcgtaaccaa gactcggatg atggacggcg
    16681 acgaatgcgc ggctgggatc atcccaatgg ggatgcacat cggcaacgta caaatggaac
    16741 ggttcgctga cgcgaagtcc accaccaaac agcaacaaag tgatcagcat gccgcgatag
    16801 tcctgctttc ctgcgacccg gaatcctttg actaacaact cctcgaaccg ctcttcgggg
    16861 aacgccggag ggcgctttgg aagcaccttc ggggtacgtt cgccccgcgt cagtcgtcca
    16921 cgttcttggg gtttggttga ccacgcgtgt ccaaggaacg ccttatcacg acgatatttg
    16981 tacgcttggt agtccaagcg tcgatcatgt gtgttgcctt catagcgagg attgaacttg
    17041 gctgcgcgcg gcgcatcctc gcgacccagc cagtcaaaga agtcgctgag ttgggtgatc
    17101 atgaagttag cttcgcgtgg tgccattcca gaccaccaga gattgcttgg gtcgtcccgt
    17161 gtgtcgggat cgacggtgcc gacatgcagg gcgtttgaga agttgcggaa catccgccat
    17221 tcctcttcgc cctggacggc attgacctcc aggtactcca agaacagttt gacgccacgc
    17281 accaactttt cttgccacgc gagactcctg cgcagcgaaa ggcagtagtc aatcaacggg
    17341 cggacagggc cttgcggtgt cattagcacc gggatttgca acgatgtacc ggtattgtcg
    17401 gaatagacct tgccgctgac cacgctaaag accacaagta gttccttcga tcaccatagt
    17461 tctggttgca ttatatgtat taaaggaggt gatagcacta tacttttggg caagtttttt
    17521 tagtttcgca aattttggtt cgaaaaaagg gggcagaacg gggaaagtat gtgttcgaaa
    17581 atgggggtca taaacggctg tgtcgtggcg cgttctgccg aggtcaaggc actgggtatc
    17641 ccgatgggcg tgccctggtt ccagcttcag gcgcaggccc gaaaacacgg catcgttgcg
    17701 tgctcctcga actacgcctt gtacggcgac atgagcaacc gggtggtcga ggtgctctcc
    17761 agtttctcgc ccaagatcga ggtgtacagc attgacgaat cgtttctcga cttcact
//
LOCUS       DAOSIT010000004        13727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_4565_length_13727_cov_10.666325, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000004 DAOSIT010000000
VERSION     DAOSIT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_13727_cov_10.666325"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLL14_00270"
     CDS             <1..585
                     /locus_tag="PLL14_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG02379.1"
                     /translation="FIYFGRRDEADNRFAAGECGMLTSSSSFYGTLHEKGKLDTGVSA
                     LPYHDDVPGTPQQTLAGGASLWVGAGQKPAEYKGVAQFVNFLMEPDLQVELSAAAGFL
                     PMTSAARAAAGSKLLKADVAGLNVAYKQLQGPGALRTLRVSEIERVRIIAEEELEAAW
                     AGKTPAKQALDVAVQRGNLVMPGASAPAAATRKK"
     gene            609..1325
                     /gene="ugpQ"
                     /locus_tag="PLL14_00275"
     CDS             609..1325
                     /gene="ugpQ"
                     /locus_tag="PLL14_00275"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOG02380.1"
                     /translation="MPRVFAHRCGGALAPENTLAGLRIAARLGLRAVEFDVMLSADAS
                     PWLLHDESLQRTTNGSGRVCATRDAQLRVLDAGAHQHPAFAGEAIPTLAAAAHACRQL
                     GLSANVEIKPAAGFEALTGEVVARQVRTLWAGAALPLVSSFSETALAAARAAGPELPL
                     GLLYERPPADWLAGAREFAAQTLHCAAEFLEDDLLCEARAAGIPVLCYTVNDPLVAAA
                     LLRRGVTAVFSDRIDCVGED"
     gene            1478..4093
                     /gene="alaS"
                     /locus_tag="PLL14_00280"
     CDS             1478..4093
                     /gene="alaS"
                     /locus_tag="PLL14_00280"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG02381.1"
                     /translation="MTSAEIRDKFLKFFASKGHQIVASSSLVPHADPTLLFTNAGMNQ
                     FKDVFLGFDKRPYLRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     LDAIRYAWELLVEVYHLPPERLWVTVYAEDDEAYDIWTREIGIPAERVVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGEGIPGGPPGSPDEDGDRYIEIWNNVFMQFNRD
                     EAGVMHPLPKPSVDTGMGLERTAAVLQHVHSNYEIDLFVRLIAAAARETAASDLDSPS
                     LRVLADHIRACAFLISDGVIPGNEGRGYVLRRIIRRAIRHGYKLGARAAFFHRLLPDL
                     VAEMGAAYPELVTNQDRVSEVLRQEEVRFFETIEHGMSILEGELAAMSGKGVFNGATA
                     FKLHDTFGFPLDLTADVCRERGVSVDVAAFDAAMAQQKEQARAAGKFRMAATLDYCGP
                     TTKFHGYESLEFSGQVLALYRDGSPVDALREGDLGVVVLDETPFYAESGGQVGDRGVL
                     QSTQGIFAVEDTQKIQAAVFGHQGVVRTGLIRVGDAVAARVDVLARARTVRNHSATHL
                     MHKALREVLGGHVQQKGSLVDAEKTRFDFAHNAPMSADQIRRVEVMVNNEILANAATE
                     ARIMPIEEAQRSGAMMLFGEKYGDEVRVLTIGSSKEFCGGVHVRRSGDIGLFKIVSET
                     GVAAGIRRVEAVTGDNALALVQQQQATLAEAAAAFKAPLAEVPAKLAQVLDNVRVLER
                     ELARLKARLASAQGDELLAQATEVKGARVLAAVMDGADVNALRATIDKLRDKLKSAAV
                     VLASTSDGKVTLIAGVTADLTGKVKAGDLVNMVARQVGGKGGGRPDMAQAGGSDPAGL
                     PAALASVRAWVEQRL"
     gene            4122..>4377
                     /locus_tag="PLL14_00285"
     CDS             4122..>4377
                     /locus_tag="PLL14_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOG02382.1"
                     /translation="MQHFASDNYAGICPEALAALLAANDGHVRSYGDDEWTLRAADRL
                     RELFETDCDVYFVFNGTAANSLALAALCQSYHSVICQQLAH"
     assembly_gap    4378..4477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4478..5311
                     /locus_tag="PLL14_00290"
     CDS             <4478..5311
                     /locus_tag="PLL14_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOG02383.1"
                     /translation="LAALCQSYHSVICQQLAHVETDECGAPEFFSNGAKLLAVAGEQG
                     KLTPAAIVDVVTRRRDIHYPRPKVVTLTQATEVGTVYQPDELRAIADTAHELGLRVHM
                     DGARFANAVAGLGLSPADVSWRTGVDVLCFGGTKMGLPVGEAVVFFDRRLSEDFAYRC
                     KQAGQLASKMRFLSAPWLGILENGAWLRHAAHANAMARRLAAGLAEGTGLPALFPVQA
                     NGVFVRLPSRIEEGLRARGWLFYNFIGAGGVRLMCGWDTAPETVDRLLDDIRDLSAPP
                     A"
     gene            5308..>5573
                     /locus_tag="PLL14_00295"
     CDS             5308..>5573
                     /locus_tag="PLL14_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose pyrophosphatase"
                     /protein_id="HOG02384.1"
                     /translation="MSAWRFCPLCGADLQVRAVADLPRLACGADCGFVHWDNPAPVVA
                     ALVEYHGRVVLARNQGWAAGAFGLITGFLERDEDPAAGVAREVK"
     assembly_gap    5574..5673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5747..6298
                     /locus_tag="PLL14_00300"
     CDS             5747..6298
                     /locus_tag="PLL14_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HOG02385.1"
                     /translation="MKTLIAALALAATGVASAVPVTTFVASFGGSDYYTVTDDSDGKL
                     LWTEAQAAAVGIGGNLVAINNAAEESWLRSTFGATERFWIGFTDQTSEGVWVWVSGDA
                     VTYSHWSSGEPNSGGGSYEEDYAVINWSTSGHWNDLPNSGAGFGGGHRGIIEVAKVPE
                     PAVLGLLAFGLAGLGATRRRKAA"
     gene            complement(6393..10286)
                     /locus_tag="PLL14_00305"
     CDS             complement(6393..10286)
                     /locus_tag="PLL14_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG02386.1"
                     /translation="MFPIRPGVGKKLLGLALFHAAAFALIAVIAATAFNRVEILATDI
                     ARNQMTGVLENASIARELSDALSEIDLISRSCRSGSASEDNGKRMSAALLAIAQKNQD
                     KQQADAIAALSTTATELLDQCAAIRRVLLSLDGIDRQILAELATLENLIGRILIDQTL
                     SGKTTDYLDQIMNLITGFRETVLQIGKNIAERTVLASADTKNGNPNVALIDDLNLRLQ
                     TLTASSPEIARIARRAIRLTVAYRREILELDIASDRFHDAVTRSHAAQTAVLKTMSHL
                     DEQASGRVADLGLQIHQAIAAASRWVFGLSVLIALLSFLAIAVIVRRSIKRPLQRVLQ
                     HIDAVRHGTATPDVGTPGRDEWGTIHTALLDMTAALASSRSLLQSVVDTAPIRVFWKD
                     RDLRYLGCNPAFARDAGKTHPEELLGKDDYQMGWAKEAERYRADDQAVMDSGVARLFF
                     VEPQTTPDGRTIWLRTSKVPLRDSNNQTIGILGVYEDITERKRIERRLDMAIKVTQVI
                     LWELDLTDGHLAYDRAMLPVLGLTPDEELDSLQHWVARVHPDDRTSFQEAVARAIQPE
                     AGTFDFEYRLRDSTAQYQWIHTRGSVIQRDPQGNAELAVGTSMNITERKSVQEAMRQS
                     EERSRLLAALLRLMADNVPDMIWAKDLEKRYLFANRAFCRQLLNAADTDEPLGKTDMF
                     FAQRERDRHPDDPQWHTFGELCQDSDAITLQHGGPSVFEEFGNLKGRLTYLDVHKAPF
                     LNERGMVIGTVGSARDITERKQAEAELERHRQHLEELVEQRTTELLATEARATRILES
                     SADGLYGVDGEGRITFINPAACQMLGFTAEQTMGRGAHALFHHRRPDGSPYPAAECTT
                     CQGWRQGCDSRIGDETYWHADGHAVPVTLASRPIVENGQIVGAVVSVVDVSLQRAAIQ
                     AREQALIAAENLARARSEFLANMSHEIRTPMNGVLGFAQIGRRNHQDPEKARNAFEKI
                     LTSGKQLLGVVNEILDFSKIDAGKLQVEANEMSLDEALEHALEIVTARARAKGLELRL
                     EKAPDLPPTCIGDPLRLGQILLNLLSNAVQFTATGGVTLSAARQGRELLFRVTDTGIG
                     MTADQLGFVFNPFQQADGSTTRKFGGTGLGLAICKRLVELMQGEIRVESTPGAGSMFE
                     VRLPYVRTAPHPDPREATSVTLPDEPLAGLCILLVEDDPINQMMLEVNLEDDGARLVI
                     VGDGATAVERIIADGPDTYDVVLMDIQMPVMDGYQAARRILELAPELPIIGQTAHAFD
                     EDRDKCLATGMAGHIAKPIDPQALAALILEVVARR"
     gene            complement(10303..11292)
                     /locus_tag="PLL14_00310"
     CDS             complement(10303..11292)
                     /locus_tag="PLL14_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOG02387.1"
                     /translation="MKFLCSAVLLAVILCSQVKVALAAEFKVLVVMSYEANNPWVIEI
                     REGIDSVLAASSEITYFYMDTKVSPAAGPRKGEEAYALYQRLQPDGVIVADDDAQAMF
                     VVPFLKDKVRTPVMFNGVNNSAAKYGFPATNVSGILERAHVRESLAFAKQLIPSIRTA
                     CFMTNDVPAGRALRAQVEEEKETYPVKVGAFHLVKNTRGIDALVKNGHSACDTILIDS
                     LEGIVDDTARPLNNKEVLEALRKVYSGPILGGNRYQVEQGAWAAVVKTGHEQGETSAE
                     MLLKAMRGTPVARIPVVSNVRGQRVINVTALEAHQVVLKPTILRGATLVRQQP"
     gene            complement(11405..>12531)
                     /locus_tag="PLL14_00315"
     CDS             complement(11405..>12531)
                     /locus_tag="PLL14_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG02388.1"
                     /translation="SGATAALCAAYSELGMQSALGNSCAFIDIDSDFLQERAIELLPP
                     AGVVLELMLDDVPDKATLARCHYLRDRGYTLALARYRGIDDRSRPVLPMLQVIKIDIE
                     TASESELRDLAGSLRHLPLKLLAQGVANREQMECCRRLGFELFQGRYFAQAEVVSGRR
                     LSASQAALIRLINLVGRDVDTMVIEDAFKHEPALTLNLLRVVNAVGHRGGGLAQPVTS
                     LRHAITLFGRRQLQRWLSLLLLAPHGGTADPARSPLLQVAALRGRMMELLVPVGHVND
                     HKLADLAFITGILSMMPAALGLPIEEILEQIAVEPKVRHALCQHEGLLGTILALLERF
                     DSDDAEGCDRLLSVLPGAFDRHTLNTCLSVALRWLNSTGD"
     assembly_gap    12532..12631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12632..12816)
                     /locus_tag="PLL14_00320"
     CDS             complement(<12632..12816)
                     /locus_tag="PLL14_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG02389.1"
                     /translation="MTEPALQNAFLFRQPILNHREELAGYQLSFGSGDESAAACSRTG
                     SGATAALCAAYSELGMQ"
     gene            complement(12853..>13727)
                     /locus_tag="PLL14_00325"
     CDS             complement(12853..>13727)
                     /locus_tag="PLL14_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG02390.1"
                     /translation="KIVIDCGNGVAGGIAPELFRSLGCELVELFCEVDGTFPNHHPDP
                     SKPENLQDLIRALRTTDAEIGLAFDGDGDRLGVVTKDGEIIYPDRQLMLFAADVLSRC
                     PGGQIIYDVKCTRRLAPWIREHGGEPLMWKTGHALVKAKLKETGAPLAGEMSGHVFFK
                     ERWYGFDDGLYAGARLLEILSRADDANAILKALPDSSSTPELNIGMQEGEPFALIDEL
                     QRHAHFEGAREIITIDGLRVEYADGFGLARPSNTTPVVVLRFEADNAAALERIQADFR
                     RVLATARPGLALPF"
BASE COUNT         2250 a         4235 c         4443 g         2499 t
ORIGIN      
        1 ttcatctatt tcggccgccg cgacgaggcg gacaaccgct ttgcggcggg cgagtgcggc
       61 atgctgacca gttcttcttc gttctacgga acgctgcatg aaaagggcaa gctggacacc
      121 ggtgtttcgg cgctgccgta tcacgacgac gtgccgggca cgccgcagca gacgcttgcc
      181 ggcggcgctt ccctgtgggt gggtgccggg cagaagccgg ccgagtacaa gggcgtggct
      241 caatttgtaa acttcctgat ggaacctgac ctgcaggtcg aactctcggc cgccgccgga
      301 ttcctgccga tgacctcggc cgcccgtgcc gccgccggca gcaaactgtt gaaggccgac
      361 gttgccggtc tcaacgtggc ctacaagcag ttgcaggggc ctggcgcctt gcgcacgctg
      421 cgcgtttccg aaatcgagcg ggtgcggatc atcgccgaag aggaactcga ggccgcctgg
      481 gccggcaaga ctccggccaa gcaggcgctc gacgtcgcgg tccagcgtgg caacctcgtc
      541 atgccgggcg cgtcggcacc cgcagcggca acacgcaaga agtgattcgc ccgcggctcg
      601 gctggtcctt gccgcgtgtc ttcgcgcacc gctgtggcgg tgcgctggcg cccgagaaca
      661 cgctggccgg actgcgcatc gccgccaggc tgggtttgcg ggccgtcgag ttcgacgtga
      721 tgctgtcggc cgatgcttcg ccgtggttgc tgcacgacga gtccctgcaa cgtacgacga
      781 acggcagtgg gcgggtgtgc gcgacgcgcg acgcgcaact gcgcgtgctc gatgccggcg
      841 cccaccagca cccggcattt gccggcgagg cgatcccgac tctggctgcg gcagcgcacg
      901 cctgtcggca actgggtttg tcggccaacg tcgaaatcaa gccggccgcc ggtttcgagg
      961 cgctgaccgg tgaagtcgtg gcgcgccagg tgcgcaccct gtgggcgggt gcggcactgc
     1021 cgctggtgtc gtcattctcg gaaacggcgt tggcggcggc gcgcgcggcc gggcccgaac
     1081 tgccgctcgg cttgctctac gagcgcccgc cggcagactg gctggccggg gccagggaat
     1141 ttgctgcgca gaccctgcat tgcgccgccg agttcctgga agacgacctg ctgtgcgaag
     1201 cccgcgccgc cggcatcccg gtgttgtgct acacggtcaa tgacccgctg gttgccgccg
     1261 ccctcctgcg gcgcggcgtg acggcggtgt tcagcgaccg catcgactgc gtcggcgagg
     1321 actaggggcg cttcccggca gggccgcacg gcggcttgtt gctccctcat tccaaacgac
     1381 cgccggcagc ccggccgcgg cgattcggcg gtcgcgaatc cgactttcac gttagaatcg
     1441 cagctctttg aacgaccctg aaccgggatg gatcaccatg accagcgctg aaatccgcga
     1501 caagttcctc aagttcttcg cctccaaagg tcaccagatt gtcgcttcaa gttccctggt
     1561 gccgcatgcc gacccgacgc tgctgttcac caacgccggc atgaaccagt tcaaggacgt
     1621 cttcctcggt ttcgacaagc ggccctacct gcgcgcgacg acgtcgcaga aatgcgtgcg
     1681 cgccggtggc aagcacaacg acctcgagaa cgtcggctac accgcgcgcc atcatacttt
     1741 cttcgaaatg ctcggcaatt tctccttcgg cgactatttc aagcttgacg ccatccgcta
     1801 cgcctgggaa ttgctggtcg aggtgtacca cttgccgccc gagcggctgt gggtcacggt
     1861 gtacgccgag gacgacgagg cctacgacat ctggacacgc gagatcggca tcccggccga
     1921 acgcgtggta cgcatcggtg acaacaaggg cgcacgctac gcctccgaca atttctggat
     1981 gatgggcgat accggtccct gtggaccctg cacggaaatc ttctacgacc acggtgaagg
     2041 gatccctggc ggaccgccgg gatcgccgga cgaagacggc gatcgttaca ttgaaatctg
     2101 gaacaacgtt ttcatgcagt tcaaccgtga cgaggctggg gtcatgcatc cgctgcccaa
     2161 gccgtcggtc gacaccggca tggggctcga gcgcacggcc gcggtgctgc agcacgtgca
     2221 cagcaactac gagatcgacc tgttcgtgcg gctgatcgcc gccgccgcgc gcgagaccgc
     2281 tgcgtccgac ctcgacagcc cgtccttgcg cgtgcttgcc gaccacattc gcgcctgcgc
     2341 tttcctgatt tccgacggcg tcatccccgg caacgagggg cgtggttacg tgctgcgccg
     2401 gatcatccgg cgcgccatcc ggcatggcta caagctcggc gcgcgcgcgg ccttcttcca
     2461 ccgcctgctg cccgatctgg tggccgaaat gggtgcggcc tatcccgaac tggtcaccaa
     2521 ccaggaccgc gtgtcggaag tgctgcggca ggaagaggtg cgcttcttcg aaaccatcga
     2581 gcatggcatg agcatcctcg aaggcgaact cgcggcgatg tccggcaagg gcgtgttcaa
     2641 cggtgcgacg gcattcaaac tgcacgacac tttcggtttc ccgctcgatc tcaccgccga
     2701 cgtctgccgt gagcgtgggg tgagcgtcga cgttgccgcc ttcgatgccg ccatggcgca
     2761 gcagaaggaa caggcgcgtg ccgccggcaa gttcaggatg gctgcgacgc tggactactg
     2821 tgggccgacg acgaaatttc atggttacga atcgctcgaa ttcagcggcc aggtgctggc
     2881 gctctatcgg gacggcagcc cggtcgatgc cctcagggag ggcgacctgg gcgtcgtggt
     2941 gctcgacgaa acgccgttct acgccgaatc cggcggtcag gtcggcgacc gcggcgtgct
     3001 gcagagcacg cagggcattt ttgcggtcga ggatacgcag aaaatccagg ctgccgtctt
     3061 cggtcatcag ggcgtcgtcc ggaccgggct gatccgcgtc ggcgacgctg tcgccgccag
     3121 ggtcgacgtg ctggcacgcg cgcgcacggt gcgcaaccat tcggcgacgc atctgatgca
     3181 caaggcgctg cgcgaggtgc tcggcgggca cgtgcagcag aagggttcgc tggtcgatgc
     3241 cgagaagacg cgcttcgact tcgcgcacaa cgccccgatg agcgccgacc agattcgtcg
     3301 cgtcgaagtc atggtcaaca acgagatcct tgccaatgcg gccaccgagg cacgcatcat
     3361 gcccatagag gaagcgcagc ggtcgggcgc gatgatgttg ttcggcgaga agtacggcga
     3421 cgaagtgcgg gtgttgacca tcggttcgtc gaaggagttt tgcggcggcg tgcacgtccg
     3481 ccgtagtggc gacatcggcc tgttcaagat cgtctccgag accggcgtcg ccgccggtat
     3541 ccgccgtgtc gaggcggtca ccggtgacaa cgcgctggcg ctggttcagc aacagcaggc
     3601 gacgctggcc gaagcggctg ccgcgttcaa ggcgccgctc gcggaagtcc cggccaagct
     3661 cgcccaggtg cttgacaacg ttcgcgtgct ggaaagggaa ctggcacgcc tcaaggccag
     3721 gctggcatcg gcgcaaggcg acgaactgct cgcgcaggcg accgaagtca agggcgccag
     3781 ggtgctcgct gcggtcatgg acggcgccga cgtcaatgcc ttgcgcgcga ccatcgacaa
     3841 actgcgcgac aagctgaaga gcgccgccgt cgtgctcgcc tcgaccagcg acggcaaggt
     3901 cacgctgatc gccggcgtca ccgccgacct gactggcaag gtcaaggccg gcgacctggt
     3961 gaacatggtc gcccgccagg tcggcggcaa gggcggtggt cgtccggaca tggcgcaggc
     4021 cggcggcagc gatccggcag gtttgccggc ggcgctggcc tcggttcggg cctgggtcga
     4081 gcagcggctg tgacccgcca gtgacgcgac ccgagccggc actgcagcac ttcgccagcg
     4141 acaactacgc cggcatctgt ccggaggccc tggccgcact gctggcggcc aacgacggcc
     4201 acgtccgctc gtatggcgac gacgaatgga cgctgcgtgc ggcggaccgc ctgcgcgaac
     4261 tgttcgagac cgattgcgac gtctatttcg tcttcaacgg cacggcagcc aattcactgg
     4321 cactcgccgc gctttgccag tcgtaccaca gtgtcatctg ccagcaactg gcgcacgnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc gccgcgcttt gccagtcgta
     4501 ccacagtgtc atctgccagc aactggcgca cgtcgagacc gacgaatgcg gcgcgccgga
     4561 gttcttctcc aacggcgcga agctgctggc cgtcgctggc gagcagggaa aactgacgcc
     4621 ggcagcgatc gtcgacgtcg tcacccgccg ccgcgacatc cactaccccc ggccgaaggt
     4681 ggtgacactg acgcaggcga ccgaagtcgg caccgtctac cagcccgacg agttgcgtgc
     4741 catcgccgac acggcgcacg aactggggct gcgtgtgcat atggacggcg cccgttttgc
     4801 caacgccgtc gccggtctcg gcttgagccc ggccgacgtc agctggcgga ctggggtcga
     4861 tgtgctgtgt tttggcggca ccaagatggg cttgccggtg ggcgaggcgg tggtcttttt
     4921 cgaccgccgc ctttccgaag actttgccta tcgctgcaaa caggccgggc aactggcgtc
     4981 gaagatgcgc ttcctgtccg caccgtggct gggaattctg gagaacggcg cctggttgcg
     5041 gcacgccgcg cacgccaacg ccatggcccg ccggttggcg gcgggtctgg ccgaaggcac
     5101 cggattgccg gcgcttttcc cggtgcaggc caacggcgtc tttgtgcggc tgccgagccg
     5161 tatcgaggag gggctgcgcg cgcgcggctg gttgttctac aacttcatcg gtgccggggg
     5221 ggtacgcctg atgtgtggct gggataccgc cccggaaacc gtcgatcgcc tgctcgacga
     5281 catccgcgac ttgtcggcgc cgccggcatg agcgcctggc gcttctgccc gctgtgcggc
     5341 gccgatttgc aggtgcgcgc ggtcgccgat ctgccgcgac tcgcctgtgg cgccgactgt
     5401 ggtttcgtgc attgggacaa cccggcgccg gtggtggcgg cgctggtcga atatcacggt
     5461 cgggtcgtcc tggcacgtaa ccagggttgg gctgcaggtg cgttcgggtt gatcaccggt
     5521 tttctcgaac gcgacgagga tccggccgcc ggcgtcgcgc gtgaggtcaa ggannnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntggactt cgatcatgcg agatccaccg
     5701 taagcactga gatgacccaa gaaaaccagc cagttggaga tataccatga aaacactgat
     5761 cgccgctcta gcccttgccg ccaccggcgt cgcctctgcg gtaccggtta caaccttcgt
     5821 cgccagtttc ggcggcagcg actactacac ggttaccgac gattctgacg gcaaactgct
     5881 gtggaccgag gcgcaagccg ccgccgtggg catcggcggt aacctggtgg cgatcaacaa
     5941 tgccgccgag gagtcctggc tgcgctcgac gtttggcgct accgagcgtt tctggatcgg
     6001 ctttaccgac cagaccagtg aaggcgtctg ggtttgggtc agcggagatg ccgtgaccta
     6061 ctcgcactgg agcagcggcg agcccaattc cggcggcggc agctatgagg aagactacgc
     6121 cgtgatcaac tggtcgacca gtgggcattg gaacgacctg ccaaacagcg gcgccggctt
     6181 tggtggcgga caccgcggca tcatcgaggt ggcgaaggtc cccgaaccgg ccgttctggg
     6241 ccttctcgcc ttcggtcttg ccgggcttgg tgcgacacgt cgcagaaaag cagcctgatt
     6301 gattcgcaac tcggacgttc acgggagctt cggctcccgt tctgctttcc tgggatggtc
     6361 cgagtgacat gccgtctgat gcggctgcgg gttcaccttc tggcaacaac ctccaggatg
     6421 agtgcagcca gagcctgagg atcaatgggt ttggcaatgt ggccggccat cccggtcgcc
     6481 aggcacttgt cccgatcttc gtcgaatgca tgcgcggtct gaccgataat cggcagctcg
     6541 ggtgccagtt cgagtatccg ccgcgcggcc tggtaaccgt ccatcaccgg catctggatg
     6601 tccatcagca cgacatcgta agtatccggg ccgtcggcga tgatccgctc cactgccgtg
     6661 gcgccatcgc cgacgatcac caggcgcgcg ccgtcgtctt ccagattgac ttccagcatc
     6721 atctggttga tcgggtcatc ctcgaccagc agaatgcaga ggcccgccag cggttcgtcg
     6781 ggtagcgtca ccgaagtcgc ttccctgggg tcgggatgcg gtgccgtccg cacataaggc
     6841 aggcgcactt cgaacatgct gccggcaccg ggagtgctct cgacccggat ctcgccctgc
     6901 atgagttcca ccaggcgctt gcagatcgcc agacccagcc cggtgccacc gaatttgcgc
     6961 gtcgtggagc cgtctgcctg ctggaagggg ttgaacacaa agccgagttg atccgcggtc
     7021 atgccgatgc cggtatcggt gacgcggaac aacagttccc tgccctggcg tgcagccgac
     7081 agggtgacgc cgccggttgc ggtgaactgg acggcgttgc tgagcaggtt gagcaggatc
     7141 tggcccaggc gtagcgggtc gccgatgcag gtcggcggca agtcgggtgc tttctccagg
     7201 cgcagttcga gtcctttcgc acgagcacgc gcggtgacga tttcgagggc gtgttcgagg
     7261 gcttcgtcaa gagacatttc gttggcttcg acctgcagtt tgccggcgtc gattttcgag
     7321 aagtcgagga tttcattgac gacgccgagc aactgcttgc cggaggtcag gattttctcg
     7381 aaggcgttgc gggctttttc gggatcctga tggttgcgtc tgccgatctg ggcaaagcca
     7441 agcacgccgt tcatcggggt gcggatctcg tggctcatgt tggcgaggaa ttcgctcctg
     7501 gcgcgcgcca ggttctcggc ggcgatcagc gcctgttcgc gggcctggat ggcagcgcgc
     7561 tggagactga cgtccaccac actgacgacc gcgccgacga tctggccatt ctcgacaatc
     7621 gggcggctgg ccagagtcac cggcacggca tggccgtcgg cgtgccagta ggtttcgtcg
     7681 ccgatgcgcg agtcgcatcc ctgccgccat ccctgacagg tcgtgcattc cgccgccggg
     7741 tagggactgc catccggacg gcggtggtgg aacagagcgt gcgcgccacg gcccatggtc
     7801 tgttccgcgg taaagccgag catctggcag gcagcggggt tgatgaaggt gatccggcct
     7861 tcgccatcga cgccataaag gccatcggcg ctcgattcga ggatgcgcgt ggcgcgcgct
     7921 tcggtagcca gcagttcggt ggtacgctgc tcgaccagtt cttcgaggtg ctggcggtga
     7981 cgttccagtt cagcttccgc ctgcttgcgt tcggtgatgt cgcgtgccga gccgaccgtt
     8041 ccgatgacca tgcccctttc gttgaggaag ggcgccttgt gcacatcaag gtaagtcagg
     8101 cggcccttga ggttgccgaa ctcctcgaac accgatggtc cgccgtgctg tagcgtgatc
     8161 gcatcgctgt cctggcagag ctcgccgaag gtgtgccatt gcgggtcgtc gggatgcctg
     8221 tcgcgctccc gctgcgcgaa gaacatgtcg gtcttgccca agggctcatc ggtgtcggcg
     8281 gcgttcagga gttgccggca gaacgcccgg ttggcgaaca ggtaacgctt ttcaaggtcc
     8341 ttggcccaga tcatgtccgg cacgttgtct gccatcaggc gcaagagtgc ggccagcagg
     8401 cgcgaacgct cttcgctctg gcgcatggcc tcctggacgg acttccgctc ggtgatgttc
     8461 atcgaagtac cgaccgcaag ctcggcattt ccctgcgggt cacgctggat cacggagccc
     8521 cgggtgtgaa tccactggta ctgggccgtc gagtctcgca gccggtactc gaagtcgaag
     8581 gttccggcct ccggctggat ggcgcgcgca acggcctcct ggaaggacgt acggtcgtcc
     8641 gggtgcaccc gtgcgaccca gtgctgtagt gagtccagtt cttcgtccgg cgtcagcccc
     8701 aggacgggaa gcatcgcccg atcgtaggcc aggtggccat ccgtgagatc gagttcccaa
     8761 aggatcacct gggtgacctt gatggccatg tccagccgcc gctcgatacg cttgcgttcc
     8821 gtgatgtctt cgtagacgcc caggatgccg atcgtctggt tgttgctgtc cctgagcgga
     8881 accttggagg tgcgcagcca gatcgtccga ccgtccgggg tcgtctgcgg ctcaacgaag
     8941 aacagccggg cgacgccgga atccatgacc gcctggtcgt cggcgcgata gcgctcggcc
     9001 tctttggccc agcccatctg gtaatcatcc ttgccgagga gttcttccgg atgggtcttg
     9061 ccggcgtcgc gggcaaaggc cgggttgcag cccaggtagc gcaggtcccg gtctttccag
     9121 aaaacccgta ttggcgcggt gtccacgacg gattgcagca agctccggga gcttgccagc
     9181 gcggccgtca tgtcgagcag ggcggtatga atggttcccc attcatccct gcctggcgta
     9241 ccgacatcgg gtgtcgcggt gccatggcgg accgcatcga tgtgttgcag caccctctgc
     9301 agcggccgct tgatgctgcg tctgacgatg accgcaatcg ccagaaagga cagcagtgca
     9361 atcagcaccg acagcccgaa aacccatcgg ctggccgccg caatggcctg atgaatctgc
     9421 aaacccagat ccgccacacg accggacgcc tgttcgtcca ggtgactcat tgttttcagt
     9481 accgccgtct gggctgcatg gctgcgggtg acggcatcat ggaagcgatc gctggcgata
     9541 tccaattcga ggatttcccg ccgataggcg acggtcagac gaattgcccg cctggcgatt
     9601 cgcgcaatct cgggactcga tgcggtgagc gtctgcaacc tcaggttgag atcgtcgatc
     9661 agcgctacgt tgggattgcc gttcttcgta tctgccgaag caagcacagt cctttcggca
     9721 atgttcttgc caatctggag cacggtttcc cgaaagcctg tgatcagatt catgatctga
     9781 tcgaggtaat cggttgtttt gccggacagg gtctggtcga tcagaatccg gccgatgagg
     9841 ttttccaggg tagcgagttc agcgagaatc tggcggtcga taccatccag gctgagcagg
     9901 acccgccgga tcgcggcgca ctggtcaagc agttcggttg ccgtggtgga cagcgcggcg
     9961 atggcatccg cctgttgttt gtcctggttc ttttgtgcga ttgccagcag ggctgccgac
    10021 atccgcttgc cgttgtcctc cgaagctgaa ccactacggc aacttcggct gatcaaatcg
    10081 atttccgaaa gcgcatcgga cagctcgcga gcaatcgagg cattttccag cacccccgtc
    10141 atctggttgc gggcgatgtc ggtggccaga atctcgacgc gattgaaagc cgtggcggcg
    10201 attacggcga tcaatgcaaa ggcggccgca tggaatagcg ccaacccgag cagcttcttg
    10261 ccgactccgg gacggatagg gaacacttcc tcttgctttc cgctagggtt gctgtcgcac
    10321 cagggtggcg cctctcagga tggtcggttt cagtaccacc tggtgggcct cgagggcggt
    10381 gacgttgatg acccgttgcc cgcgaacatt cgaaaccacg ggaatcctgg ccaccggtgt
    10441 acctcgcatg gccttcagca gcatctctgc cgatgtttca ccctgctcat gaccggtctt
    10501 gacgaccgcg gcccaggccc cctgttcgac ctgatagcgg ttcccgccaa ggatggggcc
    10561 actatagacc ttgcgcaggg cttcgagaac ctccttgttg ttcaagggcc gggcggtatc
    10621 gtcgacaatc ccctccaggc tgtcgatcag gatcgtgtcg caagcggaat ggccattctt
    10681 cacgagcgca tcgattcccc tggtgttctt gaccaagtga aacgccccga ccttgaccgg
    10741 ataagtctcc ttttcctctt ccacctgggc gcgcagggcc cgaccggccg gcacgtcatt
    10801 ggtcatgaag caggctgtcc ggatcgacgg gatcaattgc ttggcaaagg ccagggattc
    10861 gcggacatgc gcccgctcca gtattccgga gacattagtg gccgggaagc cgtatttcgc
    10921 tgccgagttg ttgaccccgt tgaacatgac cggcgtcctg accttgtctt tcaggaacgg
    10981 gacgacgaac atggcctgcg catcgtcgtc cgcgacgatg accccatcgg gttgcaggcg
    11041 ttgatagagc gcgtacgctt cctccccttt cctgggacct gccgccgggc tgaccttggt
    11101 atccatgtag aagtaagtga tttcgctgga tgccgccaaa accgagtcga ttccctcacg
    11161 gatctcgatc acccacggat tgttcgcctc gtagctcatg acgaccagca ccttgaattc
    11221 ggcagccagc gcaaccttta cctgggagca gagaatgacc gccagcaata ccgcggaaca
    11281 caggaacttc atcgacgaaa tcctcctact ttgtcattgt caagccggtt ctttccggcg
    11341 gtggggcaag ccaccgtttc gccggttgcc gatctgtccg gcgagcaccg cgccctgtcg
    11401 gcggctaatc accggtgctg ttcagccagc gcagggcgac gctgaggcag gtgttcagcg
    11461 tatgacggtc aaaggcgccg gggagcacgg acaacaggcg gtcgcagcct tcggcgtcgt
    11521 cgctgtcgaa gcgctcgagc agcgccagga tggtgcccag cagaccctcg tgctggcata
    11581 gcgcgtggcg caccttgggc tcgacggcaa tctgttcgag gatctcttcg atcggcagac
    11641 ccagcgctgc cggcatcatc gacaggatgc cggtgatgaa ggccaggtcg gcgagtttgt
    11701 ggtcgttgac gtgcccgacc gggaccagca gctccatcat gcgtccgcgc agggcggcga
    11761 cctgcagcag cggtgagcgg gcggggtcgg ccgtgccgcc atgcggtgcc agcagaagca
    11821 gcgacagcca gcgctgcaac tggcgccggc cgaacagggt gatcgcgtgt cgcagcgaag
    11881 tcaccggctg cgcgaggccg ccaccgcggt gcccgaccgc gttgaccacg cgcaacagat
    11941 tgagcgtcag cgccggctcg tgcttgaagg cgtcctcgat caccatcgta tcgacatcgc
    12001 gtccgaccag gttgatcagg cgaatcagtg cggcctgcga agccgagagg cggcgaccgc
    12061 tgacgacttc cgcctgcgcg aagtagcgcc cctggaaaag ctcgaagccc aggcgacggc
    12121 agcactccat ctgctcgcgg ttggcaacgc cctgcgccag gagtttgagc ggcaggtgtc
    12181 gcagcgatcc ggccaggtcg cggagttcgc tttcactcgc cgtttcgatg tcgatcttga
    12241 tgacctggag cattggcagc accggtcggc tgcggtcgtc gatgccccgg tatcgcgcca
    12301 gcgccagcgt atagccccga tcgcgcagat agtggcaacg cgccagcgtc gccttgtcgg
    12361 gcacgtcgtc gagcatcagt tcgaggacga cgccggccgg cggcagcagc tcgatggccc
    12421 tttcctgcag gaagtcgcta tcgatatcga tgaacgcgca actgttgccc agcgcgcttt
    12481 gcatgcccag ttcggaatac gcggcgcaga gtgcggcggt ggcgccgctg cnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctttgcatg cccagttcgg aatacgcggc
    12661 gcagagtgcg gcggtggcgc cgctgcccgt gcggctgcag gcggcggcgc tttcgtcgcc
    12721 gctgccgaac gacaactggt aaccggcgag ttcctccctg tggttcagga tcggctgccg
    12781 gaagaggaaa gcgttctgga gtgcgggttc ggtcatcggc gcggaggtcc cggatacgcg
    12841 gagtcgaagg aatcagaacg gcagcgcgag gccggggcgc gcggtggcga ggacacggcg
    12901 aaaatcggcc tgaatgcgtt cgagcgcggc cgcattgtcg gcctcgaagc gcagcacgac
    12961 caccggcgtg gtattcgagg gacgcgccag cccgaagccg tcggcgtact cgacgcgcaa
    13021 gccgtcgatg gtgatgattt cgcgcgcgcc ctcgaagtgg gcgtgccgtt gcaattcgtc
    13081 gatcagcgcg aagggttcgc cttcctgcat gccgatgttc agctcgggcg tcgagctgct
    13141 gtcgggcagg gccttcagga tggcgttggc atcgtcggcg cgtgacagga tttcgagcag
    13201 ccgggcgccg gcgtagaggc cgtcgtcgaa gccgtaccag cgttccttga agaaaacgtg
    13261 gccgctcatt tcaccggcca gcggcgcgcc ggtttccttg agcttggcct tgaccagcgc
    13321 gtgcccggtc ttccacatca gcggttcgcc gccatgttcg cgaatccagg gcgccagccg
    13381 gcgcgtgcat ttgacatcgt agatgatctg cccgcccggg caacgactca ggacgtcggc
    13441 cgcaaacagc atcagttgac gatccgggta gatgatctcg ccgtctttgg tcaccacccc
    13501 gaggcggtcg ccgtcgccgt cgaaagcgag gccgatttcg gcgtcggtgg tgcgcagcgc
    13561 gcggatgagg tcctgcaggt tctcgggttt cgacgggtcc ggatggtgat tcgggaaggt
    13621 gccgtcgact tcgcagaaca gttcgaccag ttcgcagccc agcgagcgaa acagttccgg
    13681 ggcgatgccg ccggcgacgc cgttgccgca gtcgatgacg atcttca
//
LOCUS       DAOSIT010000005        13176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_4775_length_13176_cov_6.510632, whole genome shotgun sequence.
ACCESSION   DAOSIT010000005 DAOSIT010000000
VERSION     DAOSIT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_13176_cov_6.510632"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLL14_00330"
     CDS             <1..243
                     /locus_tag="PLL14_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOG02391.1"
                     /translation="PGFQVEGWFAVIGPKGLSAEQVKRVNQAVVTAFNTPEVKEAMAK
                     QGNMIQVSTPEAATAHFKRELAKYAALVKKAGVEPQ"
     gene            complement(344..505)
                     /locus_tag="PLL14_00335"
     CDS             complement(344..505)
                     /locus_tag="PLL14_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02392.1"
                     /translation="MTQGVQLDFQEKAFQGVQNQVRAGHSSLDGLASQFAMHRRTLNR
                     RLLAWQTFR"
     gene            773..2491
                     /locus_tag="PLL14_00340"
     CDS             773..2491
                     /locus_tag="PLL14_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG02393.1"
                     /translation="MPAAHAVDAARPVQVLIINSFSRENSPYEVFAAQFRRDLAQRLH
                     TPVAFYDTSLDGARFDPAKDSDAFVAFLRARFGTDPPDLVVPIGPPATRFYGLNRGRL
                     FPGTPMMLAVPEERVMRGIELGPRDAATVISLDIPRLFANILQVLPATTTIAVVSGNS
                     PIERFWVNVVRQGTQALGERVRIEYLNELPLGDIEKRVASLSTNSAILFAQMYVDGAG
                     VSQDHDVALARIRAAAAAPVFGLFGNQLGKGIVGGPLMSEMVAASKTAELARAVLADE
                     NGVKPTVVRLELAAPAYDWRELKRWHIREGNLPDGSTVLFRQPTLWDEYRWAIVTGTA
                     ALLLQSALLVALVVQRSRRRLAESEARHLSGRILTAHEDERCKLARELHDDLSPRLAR
                     LAIDAARLQPVTEGPDSGGGVAMHEELSRLSQDVHGLAYRLHPTALEDLGLGDALRSE
                     CERVSKGNGLLVDQQIDALPAGIAPAVTVCLFRVAQEALRNIVRHSGAHSAHVSLAPK
                     DGGLQLLVTDDGGGFEQRIGRSAGLGIASMRERVREVGGKFSIRSEPGAGTTVQAWVP
                     LTAQNE"
     gene            2488..3132
                     /locus_tag="PLL14_00345"
     CDS             2488..3132
                     /locus_tag="PLL14_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG02394.1"
                     /translation="MKNRPRVLLADDHQMFADGLRTLLGEEFELVEYVADGVAMLEAA
                     QRLRPDVIVADITMPRLNGIDALTQLRANLPDVRVVFLTMHHDAAYARRALAAGAFGF
                     VLKHSAVQELVLALRAALEGRIFVAPSLGADLVARHQTNPGRRTDPCAALTPRQREVL
                     KLLADGKSVKQVASSLSISTRTVEFHKYTMMETVGAKGMADLIRIAIRYGLVSE"
     gene            3539..6373
                     /locus_tag="PLL14_00350"
     CDS             3539..6373
                     /locus_tag="PLL14_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOG02395.1"
                     /translation="MITIGFKALRVAAAFVTALALLPSQAQQARLDEVKATAEEAYLY
                     GFPMIVGYDVMNKFFIDRDSGQFKAPINTLSNEARVFTPKDTAISTPNSDTPYSMAML
                     DLRAEPMVLCMPAIEKARYYDVQLIDLYTNNFGYIGSRATGNGAGCYLVSGPEWQGEK
                     PPGIAKSFRSETQLGLVIYRTQLFNPADMDNVKKIQAGYKLQPLSTFLGKPAPPAAPA
                     INWPKLTPEMFTTGFAEYLDFLLQFAPPTGTAAVEKPMRDKFAAIGIGADRKAPPKTP
                     PSPEVKAALGEGVKEAFAKIGATAEGVGTTVNGWQIGSAAGSREFYKGNWALRAAAAK
                     LGIYGNSEAEAVYPFTRSDASGIVLDGSKHTYQMTFAPGQLPPVNAFWSITMYDGRTQ
                     LLIDNPINRYLVNSPMLPDLKKNPDGSLTLFIQRDSPGKDKESNWLPAPNRPMFVVMR
                     LYWPKDGAPSVYPLGKGSWKPPAIVPVRNLNALAVTRLGDKSLENIVRTDERYGHDGV
                     FQGPRGWGYWNHLEYPKPIQNPNLWPDTQSTYFLGRLELPAGSVMTMDFRYPQARYFQ
                     FALYRWERNTFVAADAIAGPEIEPEPGSTNPFRVGANRLGERRDFKLRIVADDAPVAP
                     KKRAPNTVYAGKAGGQIEGVIRIYLSDQGRDGAGWGPADASYGSRGFPTYSATLANGT
                     KLSAADVVKQMARPMTGATKQPLTAEQWEEIVNAKTNDPTLDPATAPARKNPQWEKFW
                     TLRYSVLGAFKSPEDRAKIPFAGAMEGGGDPSTQYLITYLSRKFGPVYVMRGKMPTFP
                     NTYAGAGGKGLEVMPDAQTQYWSLVSAEAAPSGQIVDGLTDMQVPLDADRNYTIVVSR
                     KEDRPSNATLANGIAWMEWSPRGEGVDGPKNRADFGMLMLRIMANNPSWTQSPDKVTK
                     PGMEAAVMGPYLPKGEYTTKAAFEANGLKK"
     gene            6429..8060
                     /locus_tag="PLL14_00355"
     CDS             6429..8060
                     /locus_tag="PLL14_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02396.1"
                     /translation="MTKSGIFKLVGLAVLVLLAVAAYRLLGTVVEPKIEHRIGEIGLT
                     PNETAANGPLPGDVSLYAKELACAQNLQTPGYFPSINGAELADAQRSGMFSCATFSGS
                     FDGPNRVFAWRSAVEYDTTSYINNRRPGELFISGGQAPPPKGTMPAGPFIAKADATTG
                     REIWRTYLDNGNVSGAWIANTNLNILPNGRVVTAWQNKVALLDGDTGLVVKQATLPTG
                     PTPAIDASYKHLTIAPDGTLILKDQTRPSGCKLQGTMAIIQCNEPGMKQGNSNLVAVH
                     PDTLDVLDSIALPEPATVPHVITMFEGRIAIYLGVNSGALRYFWDPQAKKLSQDTSWV
                     VKPMKEGQTTSDAPSILGDWIVLQTNGIGSETVASSIVAVNQKDSKRMQVIFPFGELK
                     KGEWSWAPPKPQTDPENSMIYSADMGVGKVAGIKIDQATGEMKTVWVVDDTTNAFQPL
                     IGPKDKRVMLLSNARKNVEKEPIKLALFTGNYKEQVTWRDAATGRIIAQSDFFEPLSI
                     GALITPGFGGRVYFPTGKGFITLQVMPAAAPPASK"
     gene            8142..8885
                     /locus_tag="PLL14_00360"
     CDS             8142..8885
                     /locus_tag="PLL14_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuromedin U"
                     /protein_id="HOG02397.1"
                     /translation="MSAEELAKLAQNPVGNLVSVPFQNNTNFNTGPLSGTQNILNIQP
                     VIPVNVNKDWNIITRTIMPLIWQPASLPGQGDTFGLGDIQLSAFLSPAEPGPGGLIWG
                     AGAIVQMPTNTSDMLGNKNWGLGPTAVVLKLEKGSPWVYGALFNNIWSLSSDNQGGRY
                     NNFLLQPFLNYNFPSGMYLTMAPIITANWEAASSQRWTVPIGGGIGKIFHLGKLPVNT
                     QLSGYYNVVRPDGGPNWQLRLQVQFMFPK"
     gene            8934..9746
                     /locus_tag="PLL14_00365"
     CDS             8934..9746
                     /locus_tag="PLL14_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02398.1"
                     /translation="MRQRINTRFANCLLAIGLFGLVPVGAQAQAANDKWQWELGVYGW
                     VPAIGGATSFPSGASASIDVSARDVIDALKMTFMGQIEARKGQWGVWSDVVYADLGGS
                     KDGSRDFTVGGHPVGASANLGLDVKSTVWSLAGIYNLTSKPENTTDLLFGARMLNMKQ
                     TLNWSLAGSVPELPTRSGQASVDGTNWDAIVGLKGRYYLGAERKWFLPYYVDVGTGQS
                     KLTWQINAGVGYTFDWGSVVATWRYLDYNFKSGDALQGMNMNGALIGVAFQF"
     gene            9802..10080
                     /locus_tag="PLL14_00370"
     CDS             9802..10080
                     /locus_tag="PLL14_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02399.1"
                     /translation="MIKKITIAAVALVAGVAVAQQYPMLDNVANKVVQKYQGMSCEQL
                     WAQKAQPKSAEEQRVIGLLKSDPAMRTEFLNRVAGPITNKMFECGMVP"
     gene            10242..10433
                     /locus_tag="PLL14_00375"
     CDS             10242..10433
                     /locus_tag="PLL14_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02400.1"
                     /translation="MPLLIEQRAIDLIKAASARLATAKAMSFAAGVDVEYPSKIGPPL
                     AYPVRYDVAMQRFRKVLIS"
     gene            complement(10503..11552)
                     /locus_tag="PLL14_00380"
     CDS             complement(10503..11552)
                     /locus_tag="PLL14_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG02401.1"
                     /translation="MSIKASADLLPPDSGMIRVGPLLVIPGLLREFGVDPVTVFEGVG
                     LPLATLADPEETIPFRLMCHLLAACVGATACPHFGLLVGQHNGIGSMGLIGFLAMQAP
                     DAGTALREMIQHTDLHDRGSMVRLSVAEGTATLRFDIIKPSVTGAAEVTDGALALLRN
                     TLLALCGRECKLLRVDLRHPRPEDVRPYQQTFQAPLSFGAEQNALVFNAKWLDSPVQQ
                     ADPLMRRYLRDRVNESIRRVHLDFQDKVFQSVLDQVREGSSSLDALANRLAMHRRTLN
                     RRLELCGTSFRALKSQARREIACEQLRDTGNSVESVASELGYTSASAFTRAFVKWEGL
                     PPAAWRRKAALPAKT"
     gene            11735..13132
                     /locus_tag="PLL14_00385"
     CDS             11735..13132
                     /locus_tag="PLL14_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOG02402.1"
                     /translation="MSLLCLAIPGLLPVSARAAQPDLGAIEAVRIATEAYIYGYPLVT
                     FDMARKQQTNVVQADAEHAPMGQMIRMRSYPAVDNHCCAAPNADTLYTEAWLDVSKEP
                     VVFSIPDMGNRYYMMPMLSGWSEVIKVAASTTTGSKAQAYAITGPGWSGTLPKGVTQV
                     KSPTGMVWILGRIYSTGTPEDYKAVHALQDQFSVVPLSAWGKPYTPPPGVVDPAFDMK
                     TAVRKQVNALDIEAYFSRLAQLMKTNPPTAQDAAMVAQMARIGLVPGKDYDPSGLGAI
                     DREVLKAVPKLALVEMGIHLKRQKTTNGWLFFTKGVGNFGTDYLTRGMANLLGPGWNR
                     PQDAVYPLSQKDAKGDAYDGSKANYVVRFAKGQLPPADAFWSLTMYDSDFFFVPNALN
                     RYDLAQRDKLITNADGSIDMYLQAESPGKDKEANWLPAPKGKFQLVMRLYAPRKTAPS
                     VLDGSWTPPPVRKAP"
BASE COUNT         2563 a         4195 c         4183 g         2235 t
ORIGIN      
        1 ccgggctttc aggtcgaggg ctggtttgcg gtcatcggcc ccaagggcct gagcgcggag
       61 caggtgaagc gcgtgaacca ggccgtcgtg accgcgttca acacgccaga ggtcaaggag
      121 gcgatggcca aacagggcaa catgatccag gtctccaccc cggaggctgc gacggcccat
      181 ttcaagcgcg aactggccaa gtacgcggcg ctggtgaaga aggcgggcgt cgagccgcag
      241 taggtcgtga ctgcagcggc gtcatagcga atcagcaaag tcctgatttc atagcgtcaa
      301 acgaccattt aacgctggga actggccaaa tattcagaaa ttcttatctg aaggtctgcc
      361 aggccagcag gcgccggttc agcgttcgcc ggtgcatggc gaactggctg gcgagtccat
      421 ccagcgaact gtgaccggct cgcacctggt tctggacgcc ctggaaggcc ttctcctgaa
      481 aatcaagctg aaccccttgg gtcatctggt cgacgctggc cgccaggtac tcgcacctga
      541 agtcgaagca tggtgttgga aacgaacctt tacactaccc ggcgggcagg cggatgctgc
      601 gcgaatcctt cggcggtgcg ggtgccttgc tcataccgca agcatgaccc aatcgttggc
      661 atatgaccgt tgcgcttcgt tcgtccgcgt cctgtggcag agtttcgtcg ctcgggcgag
      721 gctggtacgg tttggcatca gcatcagcat cggcatcgct tgcctggcga ccttgcctgc
      781 tgcccacgca gtcgacgctg cgcgaccggt acaggtcctg atcatcaact ctttcagtcg
      841 ggaaaactcg ccctacgaag tcttcgcagc tcagttccga cgcgatctgg cccagcgcct
      901 gcacacaccg gttgccttct acgacacgtc cctcgacggc gccaggttcg atccagccaa
      961 ggactccgat gccttcgtgg catttctgcg cgcccgcttc ggtacggatc cgccggacct
     1021 cgtggtcccg atcggtccgc cggctacgcg gttttacggc ttgaaccgcg gtcggctgtt
     1081 tccgggcacg cccatgatgc tcgccgttcc agaggagcgt gtcatgcggg gcatcgaact
     1141 cgggccacgc gatgccgcga cggtgatctc cctggacatt ccccgcctgt tcgccaatat
     1201 tcttcaggtg ctgcccgcga cgacgaccat cgctgtggtg agcggcaatt cgccgatcga
     1261 acgcttctgg gtgaatgtcg tgcgccaggg gacgcaggcc ctcggcgagc gcgtgaggat
     1321 cgaatacctg aacgagctcc ccctcggcga catagagaaa cgcgtcgcaa gcctgagcac
     1381 gaattcggcg atcctcttcg cccagatgta tgtcgacggc gctggcgtct cccaggacca
     1441 cgacgttgcg ctggctcgta tccgtgctgc cgctgctgcg ccagtcttcg gccttttcgg
     1501 caaccaactc ggcaagggca tcgtcggtgg gccgttgatg tcggaaatgg tggcggcgtc
     1561 gaagacggca gaactggccc gtgccgtgct ggccgacgaa aacggcgtca agccgacggt
     1621 tgtccgtttg gaactcgctg cgccggcgta cgactggcgc gagttgaagc gatggcatat
     1681 ccgggagggc aacctgccgg atggaagtac ggtcctcttc cgccagccga cgctgtggga
     1741 tgagtaccga tgggccatcg tgacgggtac cgcagcgttg ctgctgcaat cggccttgct
     1801 ggtggcactg gtcgtccaac gatcgcgccg gcggcttgcc gagagcgaag cccggcactt
     1861 gagcggccgc atcttgacgg cgcacgagga cgagcgttgc aagctcgccc gcgaattgca
     1921 cgacgacctg tcgccgcggc ttgcgcgtct ggccatcgat gcggcacgcc tgcagccggt
     1981 gacggaaggt cccgattcgg gcgggggcgt agccatgcac gaggaactct cgaggctcag
     2041 ccaagatgtg catggcctag cctatcgact tcacccgact gctctcgaag atctagggct
     2101 cggcgacgcg ctgcgctcgg aatgcgagcg agtaagcaaa ggcaatgggc tactcgtcga
     2161 ccagcagatc gacgcgctgc ccgcaggcat tgccccggcc gtgacggttt gcctcttccg
     2221 cgttgcgcaa gaggcactgc gaaacattgt ccggcactct ggggcgcact ccgcgcatgt
     2281 gtcgctcgcg ccgaaggacg gtgggctcca actgctcgtt accgatgacg ggggcggctt
     2341 cgagcaaaga attggccgca gcgccggatt gggcattgcc agcatgcgcg agcgcgtgcg
     2401 agaggttggt ggcaagttca gcatccgaag tgaaccgggc gcgggaacga ccgtgcaggc
     2461 atgggttccc ctgacagccc agaacgaatg aagaaccgac cgcgcgtctt actcgctgat
     2521 gatcaccaga tgttcgcgga cggcctgcgg acgttgctcg gtgaggagtt tgaactggtc
     2581 gaatacgttg cggacggtgt ggcgatgctg gaggcggcac agcggctacg accggatgtg
     2641 atcgtcgccg acatcacgat gccgcgcctc aacggcattg acgcattgac gcagttgcgt
     2701 gcaaatctac ccgatgtgcg ggttgtcttc ctgacgatgc accacgatgc tgcttatgcg
     2761 cgcagggcgc tggccgccgg agcttttggg ttcgtgctca agcactccgc ggtccaggaa
     2821 ctggttctcg ccctgcgcgc tgcgctggag gggcgcatct tcgtcgcgcc cagccttggc
     2881 gccgatctgg tggctcgcca ccagacaaac ccgggtcgac gcaccgaccc gtgcgcagcg
     2941 ctcacgcctc ggcaacggga agtcctgaaa ctgctcgccg atggcaagtc ggtcaagcag
     3001 gtcgcatcca gtctatccat ctccacccga accgtcgaat tccacaagta cacgatgatg
     3061 gagacggtgg gtgccaaggg catggccgac ctgatccgca tcgcgatcag gtacggcctc
     3121 gtcagcgaat agcccttttc caggtgccgc ttcgcgccct agtaaatttt ctaggagctt
     3181 tcttggcggc acttcggtac agtgcgctgg cgccgaggcg catggcgatt ggatgagctc
     3241 gacagattgc gcccttgcgg aaagccttgg attccacaaa acaacttatc tcgttgatgc
     3301 attcgcaggc gatgctggcg tgcaatcgcc ggtacccggc aagttgtggg tacggcatgc
     3361 cgccgacacc gtgcaagggt ctgtgtcaat tgacacgtcg atccccgata agtcgggata
     3421 ggccgagttc cctgccggta gcggctggcc gagcccgcgg ggctggcgca cgttacgggc
     3481 cgtcgatcgc gctcggccgg gtgggtgcac cctcatttaa gctaggaagg aacaaggaat
     3541 gatcacgata ggtttcaagg cactgcgggt ggccgcggct tttgtcacag cgctcgcgct
     3601 gctgccgagc caggcgcaac aagccaggct tgacgaggtg aaagccaccg ccgaggaagc
     3661 ctacctgtac gggtttccga tgatcgtcgg ctacgacgtc atgaacaaat tcttcatcga
     3721 tcgcgactcg gggcagttca aggcgccgat caacacgctc agcaacgagg cgcgcgtctt
     3781 cacgccgaaa gacacggcga tcagcacgcc caacagcgac acgccgtact cgatggcgat
     3841 gctggacttg cgcgccgagc cgatggtgct ttgcatgccg gcgatcgaga aggcgcgcta
     3901 ttacgacgtg caactcatcg acctctacac caacaacttt ggctacatcg gtagccgcgc
     3961 caccggcaac ggcgcgggtt gctacctcgt cagcgggccg gagtggcagg gggaaaagcc
     4021 gcctggtatc gccaaatcat tccggagcga gacgcagctc ggtctcgtca tctaccgcac
     4081 gcagctcttc aaccctgccg acatggacaa tgtcaagaaa attcaggcgg gctacaagct
     4141 gcagccgctc tcgacgttcc tcggcaagcc cgcgcccccc gccgcaccgg cgatcaactg
     4201 gccgaaactg acgccggaga tgttcacgac cggcttcgcc gagtacctgg actttctgct
     4261 gcagttcgct ccgccgacag gcaccgccgc ggtcgagaag ccgatgcgcg acaagttcgc
     4321 cgcgatcggc atcggcgcgg accgcaaagc gccgccgaag acgccgccct cgccagaggt
     4381 gaaggcggca ctcggcgaag gggtcaagga ggcattcgcg aagatcggcg caaccgccga
     4441 gggcgtgggc acaacggtca acggttggca gatcgggtcc gccgccggca gccgcgagtt
     4501 ctacaagggc aactgggcct tgcgcgccgc cgccgccaag ctcggtatct acggcaacag
     4561 cgaggccgag gccgtctatc ctttcacacg cagcgacgcg agcggcatcg tcctcgacgg
     4621 gagcaagcac acctaccaga tgacgttcgc gcccgggcag cttcccccgg tcaacgcgtt
     4681 ctggtcgatc acgatgtacg acgggcgcac gcagttgctg atcgacaacc cgatcaatcg
     4741 ctatctcgtc aattcgccga tgctcccgga tctgaagaag aacccggacg gctcgctcac
     4801 gctcttcatc cagcgcgact cgccgggcaa ggacaaggaa tccaactggc taccggcgcc
     4861 caaccgcccg atgttcgtcg tgatgcgcct gtattggccc aaggacggag cgccctcggt
     4921 ctacccgctg ggcaagggaa gctggaagcc gcccgccatc gtaccggtgc gcaacctcaa
     4981 cgcgctggcc gtgacgcgct tgggtgacaa gtcgctggag aacatcgtgc gcacggacga
     5041 gcgctacggg cacgacggcg tgttccaggg cccacgcggg tggggctatt ggaatcatct
     5101 cgaatacccg aagccgatcc agaacccgaa cctgtggccc gacacccagt cgacctattt
     5161 cctcgggcgc ctcgaattgc ccgcgggcag cgtcatgacg atggacttcc gctacccgca
     5221 ggcgcgctac ttccagttcg ccctttaccg ctgggaacgc aataccttcg tcgcggccga
     5281 cgcgatcgcg gggcccgaga tcgagcccga gccggggtcc acgaacccct tccgggtcgg
     5341 cgccaatcga ctcggcgagc gacgcgactt caagctgcgg atcgtcgccg atgacgcccc
     5401 ggtggcgccg aaaaagcgcg caccgaacac cgtttatgca ggaaaagccg gcggccagat
     5461 cgagggcgtg atccgcatct acctgtcgga ccagggccgc gacggcgccg gatgggggcc
     5521 agcggatgcg tcctatggaa gccgcgggtt cccgacctac tctgccacac tggccaatgg
     5581 gaccaagctc tcggctgcgg atgtcgtcaa acagatggcg cggcccatga cgggtgccac
     5641 gaagcagccg ctgacggccg agcaatggga agagatcgtg aacgcgaaga ccaacgatcc
     5701 gacgctcgac ccggcgaccg cgcccgcgcg caagaatccc cagtgggaga agttctggac
     5761 gctgcgctac tccgtgctag gtgcgttcaa gtcgcccgag gaccgggcca agattccgtt
     5821 cgcgggcgcg atggaaggcg gcggcgatcc ctcgacccag tacctcatca cctacctgtc
     5881 gcgcaagttc ggtccggtgt acgtgatgcg cggcaagatg cccactttcc cgaataccta
     5941 cgccggagcg ggcggcaagg gcctcgaagt gatgcccgat gcgcagaccc agtattggtc
     6001 gctcgtcagc gcggaggcgg cgccgtccgg acagatcgtc gacggcctga ccgacatgca
     6061 ggttccgctg gatgcagacc gcaactacac gatcgtggtc agccgcaagg aagaccgccc
     6121 gagcaacgcg acgctggcca atggcatcgc gtggatggag tggagtcctc gtggcgaagg
     6181 tgtcgatggg ccgaagaacc gagcggactt cggcatgctg atgctgcgca tcatggcgaa
     6241 caacccaagc tggacgcaga gccccgacaa ggtgaccaag cccggcatgg aagcggccgt
     6301 gatgggccca tatctgccca agggcgagta cacgaccaag gcggcgttcg aggcgaacgg
     6361 gctgaagaag tgaagaagca gcggatggcg gagcactgtg ccaagcaaaa agtaagggaa
     6421 acattcaaat gaccaaatcc ggaatcttca agctcgtagg gctcgccgta cttgtcctgt
     6481 tggccgttgc ggcctaccgt ctgctgggta cggtggtcga gccgaagatc gagcaccgca
     6541 tcggcgagat cgggctcaca ccgaacgaga ccgcggccaa cggtccgctg cctggcgatg
     6601 tgagcctgta cgcgaaggag ctggcctgcg cgcagaactt gcagacgccg ggttatttcc
     6661 cctcgatcaa cggcgcggaa ctcgccgatg cgcagcgcag cggaatgttc tcctgcgcca
     6721 cgttcagcgg tagcttcgac ggaccgaatc gagtcttcgc gtggcgcagc gccgtggaat
     6781 acgacaccac gtcgtacatc aacaaccggc gccccggcga gcttttcatt tcgggggggc
     6841 aggcgccacc ccccaaggga acaatgccgg cggggccctt catcgccaag gccgacgcga
     6901 ccacgggccg tgagatctgg cgcacctacc tcgacaatgg caatgtgtcg ggtgcgtgga
     6961 ttgccaatac caacctcaac atcctgccca acggccgggt cgtcacggcg tggcaaaaca
     7021 aggtcgcctt gctcgacggc gacaccggcc tcgtcgtgaa gcaggccacg ctgcctaccg
     7081 gcccgacacc cgccatcgat gcgagctaca agcacctgac gatcgcgccc gacggcacgc
     7141 tgatcctgaa ggaccagacg cgtccgagcg ggtgcaagct tcagggcacg atggccatca
     7201 tccagtgcaa cgagccgggc atgaagcagg gcaattcgaa cctggttgcc gtccatcccg
     7261 acacgctcga cgtactcgac agcatcgcgc tgcccgagcc ggcgacggtg ccgcacgtca
     7321 tcacgatgtt cgagggccgg atcgcgatct acctgggcgt gaacagcggc gcgctgcgct
     7381 acttctggga cccgcaggcg aagaagctgt cgcaggacac gagctgggtg gtcaagccca
     7441 tgaaggaggg ccagaccacc tccgatgcgc ccagcatcct cggcgactgg atcgtgctgc
     7501 agacgaacgg catcggcagc gagacggtgg catcgagcat cgtggcggtg aatcagaagg
     7561 attcaaagcg catgcaggtg atcttcccat tcggcgaact gaagaagggc gagtggagct
     7621 gggcgccacc caagccacag acagaccccg agaacagcat gatctactcg gcggacatgg
     7681 gtgtgggcaa ggtcgcaggc atcaagatcg accaggctac tggcgagatg aagacggtgt
     7741 gggtcgtcga tgacacgacc aacgccttcc agccgctgat cggccccaag gacaagcgtg
     7801 tgatgctgct gtcgaacgcc aggaagaacg tcgagaaaga accgatcaag ctcgcgctgt
     7861 tcaccggcaa ctacaaggaa caggtgacct ggcgcgatgc ggcgaccggc cgcatcatcg
     7921 cgcaatcgga cttcttcgag ccgctctcga tcggcgcgct gatcacgcca ggcttcggtg
     7981 ggcgagtcta cttcccgact ggcaaggggt tcatcacgct gcaggtgatg ccggcagctg
     8041 cgccacccgc gagcaagtga cgcatgactt tcgcgcgctc ttcaggcgta atcgcggcac
     8101 tggtcgcact ggcaatgcca tgcctgccgg tgcgggctga gatgtcggcc gaggaactgg
     8161 ccaagctggc gcagaaccct gtgggcaacc tcgtcagcgt gccgttccag aacaacacca
     8221 acttcaacac tgggccgttg tcgggcacgc agaacatcct gaacattcag ccggtgatcc
     8281 cggtcaacgt caacaaggac tggaacatca tcacccggac gatcatgccg ctgatctggc
     8341 agccggcatc gttgcctggc caaggcgaca ccttcggcct gggcgacatc cagctctcgg
     8401 ccttcctgtc gccggctgag cccggccccg gggggctgat ctggggcgct ggtgcgatcg
     8461 tgcagatgcc gaccaacacc agcgacatgt tgggcaacaa gaactggggc cttggtccga
     8521 ctgcagtcgt gttgaagctg gagaaaggca gcccctgggt ctacggcgcg ctgttcaaca
     8581 atatctggtc gttgtccagc gacaaccagg gtgggcgcta caacaatttc ttgctgcagc
     8641 ccttcctgaa ctacaacttc cccagcggga tgtacctgac gatggcaccg atcatcaccg
     8701 cgaactggga ggctgccagc agtcagcggt ggacagtgcc gattggtggc ggcatcggca
     8761 agattttcca cttgggcaag ctgccggtga acacgcagct tagcggctac tacaacgtcg
     8821 tgcgtcccga cggaggtccc aactggcagc tgcggctgca agttcagttc atgttcccga
     8881 agtgagtgtc tagctccttc cttttcttca acacgcttag agaggattca gcgatgaggc
     8941 agcgcatcaa cacccgattt gcgaactgct tgctcgccat cggcctgttc gggctcgtgc
     9001 ccgtcggggc ccaggcgcaa gccgcaaacg acaagtggca atgggaactt ggcgtctacg
     9061 gctgggtccc ggcaatcggg ggcgccacgt cgttcccgag tggcgccagc gcgagcattg
     9121 acgtcagcgc gcgcgacgtg atcgacgcgc tgaagatgac cttcatgggc cagatcgaag
     9181 ctcgcaaagg ccagtggggc gtttggagcg acgtggtcta tgccgacctc ggtggctcga
     9241 aggacgggtc gcgcgacttc accgtgggcg gccatccggt cggcgcctcg gccaacctcg
     9301 gcctggacgt gaagtcgacg gtatggtcgc tggccggcat ttacaacctg acctcgaagc
     9361 ccgagaacac gaccgacctg ttgtttggcg cccgcatgct gaatatgaag cagacgctga
     9421 actggtcgct ggccggcagc gtccccgagt tgccgacgcg aagcggccaa gcgagcgtcg
     9481 atggcaccaa ctgggacgcc atcgtcggcc tcaagggccg ctactatctc ggcgccgaga
     9541 gaaagtggtt cctgccgtac tacgtcgatg ttggcaccgg ccagtccaag ctcacctggc
     9601 agatcaacgc tggcgtgggc tacacgttcg actggggttc tgtggtcgcc acctggcggt
     9661 acctggatta caacttcaag tcgggcgatg cgctgcaggg catgaacatg aatggcgcgc
     9721 tcatcggcgt ggcgttccag ttctgacgga atggcaagac cacccgcttt tcttcaaccc
     9781 gcttcaagcc gaggactcac catgatcaag aagatcacca tcgctgccgt cgctctcgtc
     9841 gctggcgtgg ccgtcgcgca gcagtatccg atgctcgaca acgtcgccaa caaggtagtg
     9901 cagaagtacc agggcatgag ctgcgagcag ctctgggcac agaaagcgca gcccaagtca
     9961 gccgaggagc agcgcgtcat cggcctcctc aagagcgatc ccgcgatgcg caccgagttc
    10021 ctgaaccgcg tcgccgggcc gatcacgaac aagatgttcg aatgcggcat ggttccctga
    10081 gggcccgcca tgacctccat ccctaagacg ccgcgcgccg gccgctgggc acagatgttc
    10141 gtcggcgccg ccctgctgag cgctggggcc ctggccgaag gtaaccctgg cacgttggcc
    10201 gcgccggcaa gccgccggcc aaggcgcccg tcaaggcccg ggtgccactg ctgatcgagc
    10261 agcgcgccat cgacctgatc aaggccgcca gcgcccgctt ggcgacagcc aaggcgatga
    10321 gcttcgccgc tggcgtggac gtcgagtacc cgagcaagat cggcccgccg ctggcttacc
    10381 cggtgcgcta cgacgtggcc atgcagcgat tcaggaaagt cctgatttca tagcgtcaaa
    10441 tgaccacctt acgctgggat ttggcgtatt ttgcagaaaa tcttgtctga aggcctgcca
    10501 ggtcatgtct tggcaggcaa ggcggccttt ctccgccagg cggccggcgg caagccttcc
    10561 cacttgacaa aggcccgggt gaacgcgctg gcgctggtat agccgagttc gctggcaacg
    10621 ctctcgacgc tgttgccggt gtcgcgaagc tgctcgcagg cgatctcgcg gcgcgcctgg
    10681 cttttcaggg cgcgaaagct ggtgccgcac agctccagcc gccggttcag ggtccggcga
    10741 tgcatggcaa gccgattggc caaggcgtca agggaactgc tgccctcgcg tacctgatcg
    10801 agcacggatt gaaatacttt gtcctggaag tcgaggtgca cccgacggat gctttcattc
    10861 acgcggtccc gcaagtagcg ccgcatcagg gggtcagcct gctgcaccgg tgaatccagc
    10921 catttggcat tgaagacgag agcattttgc tctgcgccaa acgacaaggg cgcctggaac
    10981 gtttgttgat agggacgaac atcttcgggc ctgggatggc ggagatccac gcgcagcaac
    11041 ttgcattcac ggccgcacaa ggccagcaag gtgttgcgca gcagcgccag ggcgccgtcg
    11101 gtgacttcgg ccgcgccggt gaccgatggc ttgatgatgt cgaagcgcag ggtggccgtt
    11161 ccctctgcca ccgatagcct caccatcgag cctctgtcat gcagatcggt gtgctggatc
    11221 atctcgcgca aggccgttcc ggcatccgga gcctgcatcg ccaggaaccc gatcagaccc
    11281 atggagccaa ttccgttgtg ctggcccacc agcaagccaa agtggggaca ggccgtcgca
    11341 cccacgcagg cggccagcaa atggcacatg agccgaaatg gaatggtctc ctctggatcc
    11401 gcgagagtcg caagtggcag accgacgcct tcaaacacgg tcaccggatc aacgccgaat
    11461 tcgcgcaaca ggccgggaat caccagcaag gggccgacgc ggatcatccc cgaatcgggg
    11521 ggcagcaggt ccgcggaagc cttgatgctc atggttattg acctcaatca gggtacttgg
    11581 cgcaaagtat caagaactcg tcgcgcgaag tctaacgctg gcgaaccccc aacgctacat
    11641 tgttttcgtt ggtcggccgt gcctgcgtag ctggcgtccc tgaacaaaag gaggggtgat
    11701 gacgagatgg tgcaaccgat gggtggggcg ctgggtgtcc ctgctgtgtc tggccattcc
    11761 aggtcttttg cctgtgtccg cgcgcgcggc acagcctgat cttggcgcca ttgaagcggt
    11821 ccgcatcgcg accgaggcct atatctacgg ctatccactg gtcaccttcg acatggcccg
    11881 caagcagcag accaatgtcg tgcaggccga tgccgagcac gcgccgatgg gccagatgat
    11941 ccgcatgcgc agctaccccg ccgtggacaa ccactgctgc gcggccccca atgccgacac
    12001 gctgtacacg gaagcctggc tcgacgtgtc caaagagcct gtcgttttca gcatccccga
    12061 catgggcaac cgctactaca tgatgcccat gctgagtggc tggtccgagg tgatcaaggt
    12121 ggccgccagc accacgaccg gcagcaaggc ccaggcctat gccatcaccg gccccggctg
    12181 gagcggcacg ctgcccaagg gcgtgacgca ggtgaagtcc cccaccggca tggtctggat
    12241 cctcgggcgc atctacagca ccggaacccc agaggactac aaggccgtgc acgccttgca
    12301 ggaccagttc agtgtggtgc cgctttccgc ctggggcaag ccctacacgc cgccgcccgg
    12361 cgtggtggat cctgccttcg acatgaaaac cgccgtgcgc aagcaggtca acgcgctcga
    12421 catcgaggcc tatttcagcc ggctggcgca gctgatgaag accaacccgc cgacggcgca
    12481 ggatgcggcc atggtcgcgc aaatggccag gatcggcctg gtgccgggca aagactacga
    12541 cccgtccggc cttggcgcga tcgatcggga ggtgctgaaa gcagtgccca agcttgcgct
    12601 ggtcgaaatg ggcatccacc tcaagcggca aaagaccacc aacggctggc tgttttttac
    12661 caagggggtc ggcaacttcg gcaccgacta cctgacgcgc ggcatggcca acctgctcgg
    12721 cccgggctgg aaccgcccgc aggacgccgt ctacccgctg tcgcagaaag acgccaaggg
    12781 cgacgcctac gacggctcga aagccaacta cgtggtccgg ttcgccaagg gccagttacc
    12841 gccggccgac gccttctggt cgctgacgat gtacgacagc gacttcttct tcgtgcccaa
    12901 cgccctcaac cgctacgacc tggcgcagcg cgacaagctg atcaccaacg ccgatggttc
    12961 gatcgacatg tatctgcagg ccgagtcccc cggcaaggac aaggaggcca actggctgcc
    13021 ggcgcccaag ggcaagttcc agctcgtgat gcgcctttat gccccgcgca agaccgcgcc
    13081 gtcggtcctg gacggcagct ggacgccgcc cccggtgagg aaggcgccat gaccgaacag
    13141 catgggagcc cgcccgaaat ccccgagatc tggctc
//
LOCUS       DAOSIT010000006        12482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_5083_length_12482_cov_10.560554, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000006 DAOSIT010000000
VERSION     DAOSIT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 12482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12482
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5083_length_12482_cov_10.560554"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLL14_00390"
     CDS             complement(<1..596)
                     /locus_tag="PLL14_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOG02403.1"
                     /translation="MRRQLIKRYFITGLLIWVPLGITAWVLVLIVGTMDQSLRLLPET
                     IHPRNMLGFDIPGAGAILTLLVIVLTGVLAANFIGQRLVTWWERLLTSIPVVNSIYNS
                     VKQVSDTLFSSSGNAFRKALLIEYPRRGAWTIAFLTGKPGGDVLQHLDGDYVSVYVPT
                     TPNPTSGFFLMLPKAEVIELEMSVDTALKYVISMGVVAP"
     gene            complement(581..922)
                     /locus_tag="PLL14_00395"
     CDS             complement(581..922)
                     /locus_tag="PLL14_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG02404.1"
                     /translation="MPIYAYRCDSCGFEKDHLQKLGDSVLRICPQCGQATYSKQLTAA
                     GFQLKGTGWYATDFKGGNSKATPTTGKPESTDKPASTEVNTDGKAGSENKASADATPA
                     SASGASACAGS"
     gene            complement(1026..1886)
                     /locus_tag="PLL14_00400"
     CDS             complement(1026..1886)
                     /locus_tag="PLL14_00400"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOG02405.1"
                     /translation="MPGALTVDLDPTTFTVIAGVLGLLVGSFLNVVIHRLPRIMEREW
                     RAHCAELRGETPVEHERFSLARPRSQCPACGHAITAIENVPVLSWIFLRGRCSACAKP
                     ISPRYPLVEATSGLLCAFAALHFGYGWAAAGALLLIWCLIALAWIDLDTQLLPDSITL
                     PLLWVGLLFNVSGSFVDLQSAVIGAVGGYLSLWLVYWAFKLTTGKEGMGYGDFKLLAA
                     LGAWLGWQMLPLTILLSSLLGAVVGITLIVVIKRGRSVPIPFGPYLAAAGILALFWGK
                     PLTRAYIGLL"
     gene            complement(1886..>2424)
                     /locus_tag="PLL14_00405"
     CDS             complement(1886..>2424)
                     /locus_tag="PLL14_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG02406.1"
                     /translation="FGGIGGAIYAFFYTWKRSETMQIAFDRAFLRMPVFGDIIRKSVI
                     ARWTRTLATMFAAGVPLVESLESVGGAAGNYVYKAATRQIQSEVSTGTSLTTAMQNTS
                     LFPNMVNQMVAIGEESGALDAMLGKVADFFEAEVDDAVEALASLMEPMIMVVLGVLIG
                     GMVIAMYLPIFKLGAVVG"
     assembly_gap    2425..2524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2525..2910)
                     /locus_tag="PLL14_00410"
     CDS             complement(<2525..2910)
                     /locus_tag="PLL14_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG02407.1"
                     /translation="MATAARPPKKTESDVKEFVFLWEGKNKAGKVVRGEQRASSQTVV
                     QATLRRQGILVSKISKQRFKGGRQITDKDITLFTRQLATMMKAGVPLLQTFEIVGRGH
                     DNPAVGKLLLDIKADVETGSSLSQAFR"
     gene            complement(2924..4639)
                     /gene="pilB"
                     /locus_tag="PLL14_00415"
     CDS             complement(2924..4639)
                     /gene="pilB"
                     /locus_tag="PLL14_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOG02408.1"
                     /translation="MAANPQQSPLSGFARALVQAGRLKEAEAESLLAQAAAKKTSLIE
                     QLVGAKKGTFLEFARFAADKFGYPLLDLAAFDDSYIQKNAIDRKLIATHRVVPLHKRG
                     NRLAIAIADPTNLRALDEIRFQTGLAVDPVIVEENKLGPLVTKLSESVEETLKSFTSE
                     DINLEFTDEQSPEKVDDASSLEVDDAPVVRFIQKMMLDAINEGASDIHFEPYEKSYRI
                     RFRSDGILREVAAPPLVIKDKIASRIKVISRLNIAEKRVPQDGRMRLVLSKNRSIDFR
                     VSTLPTMYGEKIVLRILDPASASLGIDALGYEPEQKSVLLDAIHRPYGMILVTGPTGS
                     GKTVSLYTCLNILNRPGVNIATAEDPAEIPLPGINQVNVDDRAGLTFPIALKAFLRQD
                     PDIIMVGEIRDIETAEIAIKAAQTGHMVLSTLHTNDAPATLTRLMNMGIPTFNLASSI
                     LLITAQRLVRRLCTCKKPLDTPIETLLNAGFRESDLDGTWTLYGPGECERCKGSGYKG
                     RVGLYEVMPVTEAIQRIIMASGTELDIAIQARKEGVNDLRRSGLLKVKQGLTSLDEVL
                     GSTNA"
     gene            complement(4880..>5815)
                     /locus_tag="PLL14_00420"
     CDS             complement(4880..>5815)
                     /locus_tag="PLL14_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG02409.1"
                     /translation="YPDGEDERILRATQTILNEGLTSPILIGRPDVIAARLERIGSKL
                     RVGTHFEVVDPNSDQRYKECWTTYHQLRKRQGVTIDIAKTRLRSDNTIIGAILLKLGY
                     ADGMICGLSGRFAHHLRQVDEIIGKAPGCSTMAAMTHLLLPGRALFLCDTHVNEEPDA
                     AQLAEIALMAVDAVRRFKIRPKVAMLSHSNFGSSQSASASKVAEAARIVRARAPDLEV
                     DGEMHGESALSEAIRRQSDPESPLSGEANVLVMPNLDAANISYNLLKVTGGEGMSVGP
                     ILLGAARPVHVLAPTSSVRRVVDMTALVVVDAASK"
     assembly_gap    5816..5915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5916..6910)
                     /locus_tag="PLL14_00425"
     CDS             complement(<5916..6910)
                     /locus_tag="PLL14_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOG02410.1"
                     /translation="MENDPRQNELREAALEYHRYPTAGKIAVQPTKGLSNQIDLALAY
                     SPGVAYACHAIVDDPGTASLYTSRANLVGVITNGTAVLGLGNIGPLAAKPVMEGKGCL
                     FKKFAGIDVFDIELAENDPDRLIDMIAAMEPTLGGINLEDIKAPQCFYIEAKLKERMS
                     IPVFHDDQHGTAIISSAAILNALKVTGKNIGEVKVVCSGAGAAAISCLNLWCDLGVQR
                     ENIIVCDSKGVVYVGRDENMEPTKARYARTTDARTLSEALDGADVFLGLSAAGALEPD
                     MLRRMARQPIVFALANPTPEIMPEIARAVRSDVLMATGRSDYPNQVNNVLCFPFI"
     gene            7209..>9159
                     /gene="fusA"
                     /locus_tag="PLL14_00430"
     CDS             7209..>9159
                     /gene="fusA"
                     /locus_tag="PLL14_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG02411.1"
                     /translation="MGQTATSNLRTVALVGHGAAGKTTLAESLLAAAGAISSRGSVEK
                     GSTVCDFDPQEKEFGHSLNAALASFSWQGAQVHLIDTPGFPDFAGPAIGALAAVDTAL
                     VVISAQNGIELSSERMMRLAAARGVCRMIVINKIDAENVNLPALVGEIRERFGRECML
                     LDLPAKQGSDVVELLGHDDGESDFESVAAAHRALIDQIVEEDEDLLAKYLDEGVDPSP
                     DELHLPFEKALRAGHLIPILFVSAKTGAGILQLLDVLARLAPNPAEGNPPPFYRGEPG
                     GPVEAFQAEPDPQKHVLAHVFKVVSDPFIGKVGVFRVHQGTIRRDSQLFVGDSKRPFK
                     VGHMYRLQGKEYVEVETLVPGDLGAIAKVEEIEFDCVLHDSHDEDHIHLKPLEFPQPM
                     HGLAVHARRKADEQRLFEILHKLEVEDPCFRIERHPTTNETVIRGIGEMHLRAKLTRL
                     SQQYKLELDTKPPQIAYREAITGTAEGHCRHKKQSGGAGQFGEVMLRVEPLERGAGFD
                     FVDIVKGGVIPGVFMAAVEKGVRQALADGVVAGFPVEDLRVTVHDGKSHPVDGKDIAF
                     FTAARKATVDAIRAARPIILEPLVNIEILAPDAMTGDVTGDLSSKRGHLTGTQPRGPG
                     VMAITGQVPLAELEGYQSRLKSLTGG"
     assembly_gap    9160..9259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9260..11494
                     /gene="plsB"
                     /locus_tag="PLL14_00435"
     CDS             <9260..11494
                     /gene="plsB"
                     /locus_tag="PLL14_00435"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HOG02412.1"
                     /translation="VLPRSFFFLNRDRSLAGRARDGSSYSLLLARLISQAIADPLVDV
                     QLVPVVILWGRSPDKQDSVLKALFSETWRPPGAWRQLLAILLHGRNVLVRYNAAISLR
                     DLLQGGLAEEPALRKLSRVLRVHFRRQRQMAIGPDLSHRNTQVDAVLAGEQVRAAIAS
                     EAAIAGISAGEAKARAANFAIEIASDYSYGVVRALELFLSWLWTRLYDGTELHNFDTV
                     TRIAAGHEIVYVPCHRSHIDYLLLSYIIISKGLTPPHIAAGANLNLPLVGSLLRRGGA
                     FFLRRSFKGEPLYAAVFHEYLHLMLARGFPIEYFIEGGRSRSGRMLSPKAGILGMTVQ
                     SFIREHARPLVFVPVYIGYEKLIEGRSYLGELAGKPKQRESLWGLVTSVRNIKRVFGK
                     VHVNFGEPLELAGFLDQQQPGWSEETTASQSLWSRSATRNAAAELAKRINEAAVVNPV
                     NLIALALLATPKHTADEHALQRMIVHYQALLREAPYAPTTIPCPLDAQQVVTYAERLA
                     VVERFADPLGDLIRVRDEQAPLLAYFRNNVLHVFALPALVACLLSHNRRLDAARVTRA
                     VSGICALMRAELFLRWPPGELEAATEAVIRVLLARGLLCRSPASGRLAAPEPISQEFA
                     ELHLLGETIRPLLERHFLALALLDRQGTGQLTRQALEDNCHLLAQRLSLLYEFNTPEF
                     PEKATFSAFIANLIEADILHEDDQGLLHFDERLMTPLAHSELVLSVEARRAIRRIAGA
                     RVAK"
     gene            complement(11521..12162)
                     /locus_tag="PLL14_00440"
     CDS             complement(11521..12162)
                     /locus_tag="PLL14_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOG02413.1"
                     /translation="MSFIQHSLITIGLMVALGTFKPFFGESAVAEQPQAAATESATET
                     DTEASEVVVSKPAAEILSPRLQRVLDYVSRRYRVAPEALVPVFEAAQAVGRERRLDPL
                     LIVAIIGIESRFNPFAESASGAKGLMQVIPGYHLDKVPRGAGEQPFLDPVTNIQVGVH
                     VLEEAIRWRGSLISGLQHYGGSPEDAETEYANRVLAEKERLEQAARRGPGSRA"
     gene            12152..>12482
                     /locus_tag="PLL14_00445"
     CDS             12152..>12482
                     /locus_tag="PLL14_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02414.1"
                     /translation="MKLKVASVRRPVVFLSDNVADMLSPSLWSSRHSNGSTMDGYRRV
                     QGLEYLGDSSPEAGTLYGPKGQKTERRKMQMGSFGTSVCCRRNCSLLINLDLVNPHRK
                     SGSTAPAP"
BASE COUNT         2455 a         3793 c         3591 g         2343 t
ORIGIN      
        1 ggcgccacga cgcccatcga gatcacgtac ttcagggccg tatccacact catctccagt
       61 tcaatgacct ccgccttggg cagcatcaaa aagaaaccgg aagtgggatt cggtgtcgtc
      121 ggaacataca cgctgacata gtcgccgtcc agatgctgca ggacatcgcc accgggcttg
      181 ccggtaagga aagcgatcgt ccacgcgcca cgtcggggat actcgatcag cagtgccttc
      241 cgaaaagcgt tgccggacga cgagaaaagc gtatcggaaa cctgcttgac gctgttataa
      301 atggaattga ccaccggaat gctggtcaag agccgctccc accaggtaac cagccgctgt
      361 ccgatgaaat tggcggcgag cactcccgtg agcacgatga ccaacaaggt gagaatcgcg
      421 ccagcgccgg gaatgtcaaa acccagcata ttgcgtgggt gaatcgtttc aggcaaaagg
      481 cgcaaagact ggtccatggt gccgacgatc agcaccaaca cccaggctgt gatccccagc
      541 ggaacccaga tcagcaggcc ggtaatgaag tagcgcttga tcaactgccg gcgcacgcac
      601 tggctcccga ggcactggct ggagtcgcgt ctgcactcgc cttgttttcg gagcctgcct
      661 taccgtcggt atttacctcc gtgcttgcgg gtttgtccgt gctttccggc ttgccggtcg
      721 tgggtgtagc cttgctattg ccgcccttga aatctgtagc gtaccagccg gtgcccttca
      781 actgaaagcc cgcagccgtc aactgcttgc tataagtcgc ttgaccacac tgagggcaga
      841 tacgcaacac cgagtcgcca agcttttgca gatgatcttt ctcaaacccg caggaatcgc
      901 agcgataagc gtaaatcggc atgtcaaacc ctcgaaaagc acttgaagaa acttgaagaa
      961 tcgagattat acgcgaaccg gcggcaattc cagcttgctc gccgggcggg tgcagccgga
     1021 tggcactaca gcaaaccgat gtaagcgcgc gtcaggggtt ttccccagaa cagggccagt
     1081 atccctgccg cagccagata ggggccgaag ggaatcggta cacttcgccc gcgtttgatg
     1141 accacgatca gagtgatgcc gacaaccgcg cctagcagcg acgagagcag aatggtcagc
     1201 ggcagcatct gccatccgag ccaggcgcca agtgccgcga gcagcttgaa gtcaccgtaa
     1261 cccatgccct ccttgccggt ggtcagcttg aaagcccagt agaccaacca cagggagagg
     1321 tagccgccca ccgcaccgat cacggccgat tgcagatcaa cgaaactccc cgatacgttg
     1381 aacaacagcc caacccagag caagggcagc gtgatcgaat ccggcaacaa ctgcgtgtcg
     1441 aggtcgatcc aggccagggc aatcaggcac cagatcagca gcagggcgcc tgcagctgcc
     1501 cagccgtagc cgaaatgcag cgcggcgaag gcgcacaaca ggccgctggt ggcttcgacc
     1561 agcggatagc gcggggagat cggcttggcg caggccgaac agcgtccacg caggaatatc
     1621 cagctcagca ccggcacatt ctcgatggcc gtgatcgcat gaccgcaggc tgggcactgt
     1681 gagcgtggcc tggccagcga gaaccgttcg tgctcgaccg gcgtttctcc gcggagttcg
     1741 gcacagtggg cgcgccattc ccgctccatt attctgggca ggcgatggat gacaacgttg
     1801 agaaagctgc ccaccagcag cccgaggacg cccgcgatga ccgtaaacgt ggtcggatcc
     1861 agatcgacag tcaaggcgcc aggcattatc cgacgacagc gcccagtttg aagatcggca
     1921 gatacatggc aatcaccatg ccaccgatca gcaccccgag aaccaccatg atcatcggct
     1981 ccatcaggct ggcaagggcc tcgacggcgt cgtccacctc cgcttcgaag aaatcggcga
     2041 ccttgccgag catggcatcg agagcgccgg actcctcccc gatggcgacc atctgattca
     2101 ccatgttcgg gaacagtgag gtattctgca ttgccgtggt caggctggta ccggtgctca
     2161 cctcactctg aatctgccgt gtggccgcct tatacacgta gttgccggcg gcaccaccga
     2221 ccgattccag ggactccacc agaggaacac cagctgcgaa catggtggcc agcgtacggg
     2281 tccagcgagc gatgaccgac ttgcgaatga tgtcgccaaa aacgggcatg cgcaggaatg
     2341 cccggtcgaa agcgatctgc atggtttccg atcgtttcca ggtatagaag aaagcgtaga
     2401 tcgcgccacc gatgccgccg aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnncggaac gcttgcgaaa gcgagctacc cgtctcgaca tcggccttga tatccagcag
     2581 caacttgcca acggccgggt tgtcgtgccc gcgaccgacg atttcaaagg tctgcagcag
     2641 tggcacacca gccttcatca tcgtcgccaa ctggcgcgta aacagcgtga tatccttgtc
     2701 ggtgatctgc cgcccgccct tgaagcgctg cttggaaatc ttgctgacca ggatcccttg
     2761 ccgacgcagc gtggcttgca caactgtctg gctgctggca cgctgttcac cacgaacgac
     2821 cttcccggct ttgttcttgc cttcccaaag aaaaacaaat tccttgacgt ccgactcggt
     2881 tttcttggga ggtcttgctg cagtcgccat tcttacctct tcttcaagcg ttggtgctgc
     2941 cgagaacctc atcaagagag gtcagccctt gcttgacctt cagcaggccg gaacgacgca
     3001 ggtcattgac gccttccttt cgggcctgga tcgcaatatc aagctccgtt ccgctggcca
     3061 tgatgatgcg ctgaatcgct tcagtcaccg gcatgacctc atacagaccg acccttccct
     3121 tgtagccgct tcctttgcaa cgctcgcatt cccctggacc gtagagggtc catgtgccat
     3181 cgagatcgct ttcccgaaag ccggcattga gcagggtttc aatcggcgta tccagcggct
     3241 tcttgcaggt gcacaaccgg cgaaccagac gctgcgcggt gatcaacagg atgcttgaag
     3301 ccagattgaa tgtcgggatg cccatgttca tcaggcgggt cagcgtcgct ggcgcatcat
     3361 tggtgtgcag ggtggacagc accatgtgtc cggtctgtgc agccttgatc gcaatctctg
     3421 cggtttcaat gtcgcgaatc tcaccgacca tgatgatatc cgggtcctga cgcaggaacg
     3481 ccttgagggc tatcggaaag gtcaggccgg cacggtcatc gacattgacc tgattgattc
     3541 ccggcaaggg aatttcggcc gggtcttccg ccgtggcaat attgacccct ggcctgttca
     3601 ggatgttgag gcaggtgtag agcgaaacgg tttttcccga acctgtcggg ccggtaacca
     3661 ggatcattcc gtagggacga tggatcgcgt caagcaggac cgacttctgt tccggctcat
     3721 agcccagagc gtcgataccg agactggcgc tggccggatc cagaatccgc aagacgattt
     3781 tttcaccgta catcgtcggc aaggtactga cgcggaagtc gattgaccgg ttcttcgaaa
     3841 gcaccagacg catgcgtccg tcctgcggca cgcgtttttc ggcaatgttg agtcgtgaaa
     3901 tcaccttgat gcgcgaggcg atcttgtcct tgatgaccag gggcggtgct gccacttccc
     3961 gcaaaatgcc atcgctgcga aatcggattc gatagctttt ttcatagggt tcgaagtgga
     4021 tgtcggacgc accctcgttg atcgcatcga gcatcatctt ctgaatgaac ctgaccacag
     4081 gagcgtcatc aacttcgagg ctgctcgcat cgtccacctt ctccggactc tgctcgtcgg
     4141 taaactcgag gttgatgtcc tcgctggtaa aactcttcag ggtctcctcg accgactcgg
     4201 acagcttggt gaccagcgga ccgagtttgt tttcctcgac gatgaccggg tcaactgcca
     4261 agccggtctg aaagcgaatc tcgtcaagcg ctcgcagatt ggtcgggtcg gcgatggcga
     4321 tggccaggcg attgccgcgc ttgtgcaatg gcaccacgcg atgagtcgca atcaacttcc
     4381 ggtcaatggc gttcttctga atataggaat cgtcgaacgc agccagatcg agcagcggat
     4441 aaccgaactt gtctgcagcg aaacgtgcaa attccaggaa ggtgcctttc ttggctccga
     4501 ccagttgctc gatcagcgaa gtcttcttgg ccgctgcttg ggcgagcaga gactcggcct
     4561 cggcttcctt gagtcggcca gcttgtacca gcgcacgggc gaaaccgcta agcggtgatt
     4621 gttgcggatt ggctgccata tttcgcgata ggaccctgca acgcgttgtg aaccatcagg
     4681 tcccggctat accgagttgg cctactcgat gatgcattgc ctgagagcgt ttgtaaagca
     4741 gtcaccgcgc aggcgagcgc gaacgccagc aggcccgccc tctaccacag gcggcgatga
     4801 ctggcatatg cctcccaaac acagcgatca ccgcaatcgg cacccggcca gcatgggtca
     4861 tcgcggcaaa accacgccgc tacttgctcg ccgcgtccac caccaccagt gcggtcatgt
     4921 caaccacccg gcggaccgac gaggtcggag ccagaacatg caccggacgc gcggcgccca
     4981 ggaggatcgg tcccaccgac attccctcgc cgccagtaac cttgagcagg ttataggaaa
     5041 tattggcggc atcgagattg ggcatcacca gcacgttggc ctcaccgctc agtggtgact
     5101 ccgggtccga ttgcctgcgg atggcttcgg agagcgcgct ttcgccgtgc atttcgccgt
     5161 cgacctccag atcgggcgct ctggcgcgca cgattcgcgc ggcttcggcc actttgctcg
     5221 cggaggcact ctgtgaggat ccgaagttcg aatgcgacag catcgccacc ttcggcctga
     5281 tcttgaagcg acgcaccgcg tctaccgcca tcagcgcaat ttccgccagt tgcgcggcat
     5341 cgggctcttc gttgacatgc gtatcgcaga ggaacagcgc gcgtccgggc aacaacaagt
     5401 gggtcatcgc cgccatcgtg ctgcagcctg gcgccttgcc gatgatctca tcgacctggc
     5461 gcagatgatg cgcgaaacga ccggacagac cacagatcat gccgtcggcg taaccgagct
     5521 tgagcaggat ggcgccgatg atggtgttgt cgctgcgcag gcgggtcttg gcgatgtcga
     5581 tcgtcacccc ttggcgcttc cgcagttgat gataggtcgt ccagcattcc ttgtagcgct
     5641 ggtcactatt gggatcgacc acctcgaaat gcgtaccgac acgcagcttc gagccaatcc
     5701 tctcgagccg tgcggcaatc acatcggggc gaccaatcag gatcgggctg gtcaggcctt
     5761 cattgagaat cgtctgcgtc gcgcgcagga tccgctcatc ctcgccgtcc ggatannnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaaat gaacggaaaa cacaggacat
     5941 tgttgacctg gttggggtag tcggatcgcc cggtggccat gagcacgtcc gaacgcactg
     6001 cacgggcaat ctcaggcatg atttcgggtg tcgggttggc cagcgcgaag acgatcggct
     6061 gcctggccat gcgccggagc atatccggtt cgagggcgcc ggcggccgag agcccgagga
     6121 acacgtcggc tccgtccagc gcttccgaaa gggttctggc atcggtagtc cgggcgtaac
     6181 gcgccttggt cggttccatg ttctcgtcgc gaccgacgta aaccacgcct ttcgagtcgc
     6241 agacgatgat gttctcgcgc tggacaccca ggtcgcacca caggttgaga caggaaatgg
     6301 ccgccgcgcc tgcgccggag caaaccacct tcacttcacc gatattcttg ccggtcacct
     6361 tgagcgcgtt caggatcgcc gccgacgaga tgatcgccgt gccatgctga tcgtcatgaa
     6421 agaccggaat tgacatcctc tccttgagct tggcctcgat gtagaagcac tgcggggcct
     6481 tgatgtcttc caggttgatg ccgccgagtg tcggttccat ggcggcgatc atgtcgatca
     6541 gccggtctgg gtcattttcg gcgagttcga tgtcgaacac atcgatcccg gcgaacttct
     6601 tgaacagaca acccttgcct tccatcaccg gtttggcggc cagaggaccg atatttccga
     6661 gaccgagcac cgccgtacca ttggtgatga caccgaccag attcgcccgc gaagtgtaca
     6721 ggctggccgt gccggggtca tcgacgatgg catgacaggc gtaggccacg cctggcgaat
     6781 aagccagggc caggtcgatc tggttgctga gacccttggt gggctggacg gctatcttgc
     6841 ctgcagtcgg gtaacggtga tactcaaggg ctgcctcgcg aagttcgttc tggcgtggat
     6901 cgttttccat tctgcctcct gacgtgctgt tcatcaaagc aaaagcgcga cactttagcc
     6961 gactgacgcg gttaacacaa aactcatgtt atcgcgatat tcaccaacgc acggtgcagg
     7021 tgccgttgcg aagcagaaat acagttgcac ggcgaccgct gtaggccgtg ccgtcggtat
     7081 cgaccggggt gcggcctcca ccgctgcggg cgtggcagag gtagaatgat cggcgcacgc
     7141 gcaaccgcca tgccatgggg ctattcctgc ggtggcgacg ggcattcgac ttgaatggga
     7201 gatgatgaat gggccagacc gccaccagta accttcgcac tgtcgctctc gtcggccacg
     7261 gggccgccgg caagaccacc cttgccgaaa gcctgctcgc cgcagccggg gccatcagca
     7321 gccgcggctc cgtcgagaaa ggcagcactg tttgcgattt cgacccgcaa gagaaggaat
     7381 tcggccactc gctgaatgcc gcgctggcga gtttttcctg gcaaggggcg caggtgcacc
     7441 tgatcgacac gcccggcttc cccgatttcg ccggcccggc gatcggcgcg ctcgcggccg
     7501 tggacacggc tctggtggta atcagcgcgc agaacggcat tgagctttcg agcgagcgga
     7561 tgatgagact ggccgcggca cggggagtgt gccggatgat cgtgatcaac aagatcgatg
     7621 ccgagaacgt caatctgccg gcactggtcg gcgaaattcg cgagcgcttc ggtcgggaat
     7681 gcatgttgct tgatttgccg gccaaacagg gcagcgacgt agtggaattg cttgggcacg
     7741 atgacggcga aagcgatttc gagtcggtgg cggcggcgca ccgcgcgctg attgatcaga
     7801 tcgtcgaaga agacgaggat ctgctcgcga aataccttga cgaaggcgtc gatcccagcc
     7861 ccgacgaact gcacctgccc tttgaaaagg cgctgcgtgc tggccatctg attccgattc
     7921 ttttcgtttc cgcaaaaacc ggtgccggca tcctgcagtt gctcgatgtg ctcgccagac
     7981 tggccccgaa tccggcggaa ggtaacccgc cgccgttcta ccgcggcgaa cccggcggtc
     8041 cggtggaagc cttccaggcc gagccagacc ctcaaaagca cgtgctggcg cacgtcttca
     8101 aggtcgtcag cgatcccttc atcggcaagg tcggcgtctt ccgcgtccat cagggaacga
     8161 tccgccggga ctcgcaactc ttcgtcggcg acagcaaacg tccgttcaag gtcggccaca
     8221 tgtatcgcct gcagggcaag gagtatgtcg aggtcgagac gctggtaccc ggcgatctgg
     8281 gggccatcgc caaggtcgag gaaatcgagt ttgactgcgt gctgcacgat tcgcatgacg
     8341 aagaccatat ccacctgaag cctctcgagt ttccgcagcc gatgcacggc ttggcggtgc
     8401 atgcgcggcg caaggccgac gagcaacgcc tgttcgagat cctgcacaag cttgaggtcg
     8461 aggacccctg ctttcgcatc gagcgtcacc cgacaaccaa cgaaacggtg attcgcggca
     8521 tcggcgaaat gcacctgcgc gccaaactga cacgtctgtc gcagcagtac aaactcgaac
     8581 tcgacaccaa gccgccacag attgcctacc gcgaagccat cacgggtacc gccgaagggc
     8641 actgccggca caagaagcag agcggtggcg ctggccagtt cggtgaagtc atgctgcgtg
     8701 tggagccact cgaacgtggc gccggtttcg acttcgtcga tatcgtcaag gggggcgtca
     8761 tcccgggcgt attcatggca gccgtggaaa agggcgttcg ccaggcactt gccgacggcg
     8821 tcgtcgccgg cttcccggtc gaggatttgc gggtcaccgt acacgacggc aaatcgcatc
     8881 cggtcgacgg caaggacatc gcatttttca ccgctgcccg caaggccacc gtcgacgcga
     8941 ttcgggccgc caggccgatc atcctcgaac cgctggtcaa cattgaaatc ctcgcaccgg
     9001 acgcgatgac cggtgatgtc accggcgacc tgtccagcaa gcgtggccac ctgaccggca
     9061 cacagccgcg cggccccggc gtgatggcca tcaccggcca ggtaccgctg gccgaactcg
     9121 agggctacca gtcgcgcctc aagtcgctga ccggcggcgn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnng tcctgccccg ctcgttcttc ttcctgaatc gtgaccgcag
     9301 ccttgccggc agggctcgcg atggcagcag ctactcgcta ttgctcgccc gcctgatcag
     9361 ccaggcgatt gccgaccccc tggtcgatgt acagttggta ccggtcgtca tcctctgggg
     9421 ccgcagtccg gacaagcagg attcggttct caaggccttg ttctcggaaa cctggcgtcc
     9481 gccaggcgcc tggcgacaac tgctggccat cctgctgcat ggtcgcaatg tgctggttcg
     9541 ttacaacgcc gcaatttcac tacgcgatct cctgcaggga gggcttgctg aagaacccgc
     9601 gctacgaaaa ctgtcgcgcg tgttgcgagt ccatttccgt cgccagcggc agatggcaat
     9661 cgggcccgac ctctcgcacc gcaataccca ggtggacgca gtgcttgccg gtgaacaggt
     9721 gcgcgcggcg atcgccagcg aagcggcaat cgccggcatc tcggcggggg aagccaaagc
     9781 ccgcgccgcc aacttcgcga tcgagatcgc gtccgactat tcctatggtg tcgtgcgcgc
     9841 actcgaactc ttcctgtcct ggttatggac caggctttac gacggtaccg agttgcacaa
     9901 cttcgatacc gtcacacgca tcgctgcggg acacgagatc gtttacgtgc cctgccaccg
     9961 cagccatatc gattatctgc tgctgtctta catcatcatc agcaaagggc tgacaccacc
    10021 gcatatcgct gccggtgcca acctcaacct gccactggtc ggatcactgc tgcgccgcgg
    10081 tggtgccttt tttctgcgcc gcagtttcaa gggcgagccg ctatatgcgg cggtgttcca
    10141 cgaatacctg catctgatgc tcgcgcgagg tttcccgatc gagtatttca tcgaaggtgg
    10201 gcgcagccgc agtggacgga tgctaagccc aaaagccggc atcctcggaa tgacggttca
    10261 gagcttcatt cgcgagcacg cgcggccgct ggttttcgtt ccggtgtaca tcggttacga
    10321 gaaattgatc gagggccgca gctacctcgg tgaactggcc ggcaagccca agcaacgcga
    10381 atcgttgtgg ggccttgtca cgagtgtacg caacatcaaa cgcgttttcg gcaaggtaca
    10441 cgtgaatttt ggcgaaccgc tggaactcgc cggctttctc gaccagcaac agcccggctg
    10501 gagcgaagaa accacggcca gccaatcgct atggtcgcgc agtgcgacgc gcaatgcggc
    10561 agcggagctc gccaagcgca tcaacgaggc ggccgtagtc aatccggtga acctgatcgc
    10621 actcgcgctg ctcgccaccc ccaagcacac ggccgatgaa cacgcactgc agcggatgat
    10681 cgtgcactac caggcactgc ttcgcgaagc accttatgcg ccgacgacga ttccctgccc
    10741 gctcgacgcg cagcaggtgg tgacctacgc cgagcgactc gcagtcgtcg agcgtttcgc
    10801 cgatccactg ggcgatctga tccgcgtgcg tgacgagcag gcgcccttgc tcgcctattt
    10861 tcgcaacaac gtactgcatg ttttcgccct gccggcactg gtcgcctgcc tgctgagtca
    10921 caaccgccgc ctcgatgccg cgcgcgtgac acgtgcggtg agcggcattt gcgccctgat
    10981 gcgcgcggaa ctgtttctcc gctggccacc cggcgaactg gaagccgcca ccgaagcagt
    11041 cattcgtgtc ctgctcgcgc gcgggctgct gtgtcgatcg ccagcatcgg gaagactcgc
    11101 cgccccggaa ccgatcagcc aggaatttgc cgaactgcac ctgctcgggg aaaccatccg
    11161 ccccctgctg gaacgtcact tcctcgccct ggcgctcctc gatcggcagg gcaccggcca
    11221 gctcacccgc caggcgctcg aagacaactg tcacctgctg gcgcagcggc tatcgctgct
    11281 ctacgaattc aatacgccgg aattccccga gaaggccacc ttttccgcct tcatcgccaa
    11341 cctgatcgag gccgacattc tgcacgagga tgaccagggc ctgctgcact tcgacgagcg
    11401 tttgatgacc ccactggcgc actcggaact cgtgctctcc gtcgaagcgc gacgcgccat
    11461 tcggcgtatc gcgggtgccc gcgtggccaa gtaaccgcgc atgcagaaat cccgaaatgc
    11521 tcaggcacgg ctacccggac cgcgccgggc cgcctgctcg aggcgctcct tctcggccag
    11581 aaccctgttc gcatactcgg tttcggcatc ctcgggcgag cccccataat gctgcagccc
    11641 ggaaatcagg ctgccacgcc agcgaatcgc ttcctcaagg acgtgtacgc ccacctgaat
    11701 gttggtaacc ggatcgagaa acggttgctc gccagctccg cgcggcacct tgtcgaggtg
    11761 gtagccgggg atcacctgca tcagcccttt ggcaccactc gcactttcgg caaaggggtt
    11821 gaaacgggac tcgatgccga tgatggcgac gatcaacagc ggatcgagac gccgctccct
    11881 gcccacggcc tgcgctgcct cgaagaccgg caccagcgct tccggggcaa cccggtaacg
    11941 acgcgagaca tagtccagga cacgctgcaa tcgcggcgaa agaatttcgg cagcaggttt
    12001 actgacgacc acttcagagg cttcggtgtc ggtttcggtc gcagactcgg tagccgccgc
    12061 ttgcggctgc tcagccaccg cactttcgcc gaaaaacggc ttgaatgtcc ccagcgcgac
    12121 catcagtccg atggtgatca ggctgtgttg tatgaaactc aaggtggcgt cagtgcgacg
    12181 cccggttgta tttctcagtg ataatgttgc ggacatgctc tctccttcac tttggtcgtc
    12241 gcgccactca aacggctcga cgatggacgg ttacaggcgg gtgcaaggcc tcgaatactt
    12301 gggggatagt tcccctgagg ccggaactct gtacggcccg aaaggccaga aaacagagcg
    12361 gcgaaaaatg cagatgggca gcttcggtac atcggtttgc tgccggagaa attgcagtct
    12421 attgattaat ctcgatttgg tcaacccgca tcgaaaatcc gggagcacag cgcctgcacc
    12481 tg
//
LOCUS       DAOSIT010000007        12337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_5153_length_12337_cov_6.769663, whole genome shotgun sequence.
ACCESSION   DAOSIT010000007 DAOSIT010000000
VERSION     DAOSIT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5153_length_12337_cov_6.769663"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..603)
                     /locus_tag="PLL14_00450"
     CDS             complement(7..603)
                     /locus_tag="PLL14_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG02415.1"
                     /translation="MTNESLFQPVRTRRGFEAVCDRIREQLMSGELKPGDQLPGEREL
                     AERFGIGRAAARDALRSLEVSGVVEARKGIQGGFFIRNGGSGGLAQTVQDMVSLAQAS
                     LENVMEARTEIICVAIRLACPRATNEEFDTIQRDIDLHADLFRLGRASRNTQSVIEFY
                     RLIAQATHNPLVLMMVDALSEVYRGLLVFSAISDGTKS"
     gene            669..1829
                     /locus_tag="PLL14_00455"
     CDS             669..1829
                     /locus_tag="PLL14_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG02416.1"
                     /translation="MDLDLTPEQRAFRDEVRTFIREKLPSEIRERLRAGHPPRKQDTV
                     AWQRILNERGWAAPHWPKRYGGADLGPMERLILLDEAYRAPAPLPQVFNVGMLGPVLM
                     KFGTPAQCDHFLPRLANLDIWFCQGFSEPGSGSDLASLRTAARREGDHYIVNGQKIWT
                     TTAHLADWVFALVRTDTEARKQEGISFLLIDLRTPGITIRPIHSIDGEHHLNEVFFDE
                     VRVPVGNLVGQENKGWDCAKFLLSNERTGIANVGLCRERLDYARELAGRYTQAGKPLL
                     DDPKLRADIAMLEAETRALEITNWRFLLTDSEQKRLPAFASVLKLKGTELQQEINALL
                     ARIVGPGGLERRSADEVTHGPIAPRYFYSRAASIYGGTTEVQKDILAKAIVG"
     gene            1837..2973
                     /locus_tag="PLL14_00460"
     CDS             1837..2973
                     /locus_tag="PLL14_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG02417.1"
                     /translation="MNFELNEDQAALAESLSRLLADRYGFEKRRALTASGAAHDEKTW
                     AGLAELGLTALPVPEALGGFGGGARDLLPAMQAFGRSLSMEPFLASAVLAGTALRLGA
                     DEAMQTRLLPGMASGEIVSAWAHDEPAGRHAPLWVETRAERKDGGWKLDGIKSNVPAG
                     GLARYFVVSARIAGAPADANGCALFLVDAKDAGVALREFRLVDDTAAGELVLTQAAAQ
                     PLANPEDSTRTRLAIQGTVAAGIAAVCADMSGAAQAAFDLAMDYLRTRKQFGRLIGEN
                     QALRHRASEMLVSLEMARSMAMAAAVAADDASAEDSAPDLHRAKLSVARNARNVALGA
                     IQLHGGIGMTEEYAVGHYLRRIHVLDQMFGDADAHAARLAAALL"
     gene            2985..4517
                     /locus_tag="PLL14_00465"
     CDS             2985..4517
                     /locus_tag="PLL14_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOG02418.1"
                     /translation="MSITPMIYLDGRGVTRDEVLRVAQQAATGFEQLGVSEGDTVALL
                     LRNDFAFFEVQQAAAAVGAYGVPLNWHGRTEEVIYVLEDAKPKVLVAHADLLAPIRAL
                     VPAGIRVFVVPTPPEIQQRYGVSTELARPLPGDTVWREWAERFEPLQAPPRRARATMI
                     YTSGTTGHPKGVKRQPATPEESKAYVELFASVYGLKPGMRALVCGPLYHASPNGYGRQ
                     ALMTADVLVLQSKFDPEETLALIEKHRIDNAVMVPTMFVRMLKLPPEVRNRYDVRSLR
                     WVTHTGAPCPREVKKDLMDWWGPVVYEGYGGTEVGTATLATPQDWLEHPGCVGMPTPG
                     TRMAFYDEEGNRLPEGEIGEIFARVPAYADFTYLNHEEKRKSVERDGLISLGDVGYMK
                     EGRLYLCDRRSDMVIFGGTNIYPAEIEMVLTQCPGVKDCAVFGIPDDDFGESLAAAVE
                     RMPESQVTPEEIRSYLLERLAKFKVPKRIDFHESLPREDSGKIFKRRLRDPFWQNAGR
                     KI"
     gene            4528..4764
                     /locus_tag="PLL14_00470"
                     /pseudo
     CDS             4528..4764
                     /locus_tag="PLL14_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            4991..5962
                     /locus_tag="PLL14_00475"
     CDS             4991..5962
                     /locus_tag="PLL14_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOG02419.1"
                     /translation="MKFTRRQTLAAALGSLGAGVLPRAQAQTQPFPSQVIKFVIPTPA
                     GGDHDTMMRIIGQKLTDAWGQPCIVESRSGASGAIAAATVSQAPADGYTVLIGYSALL
                     SNTVLMPKPGYRLEDLQPVGMLALTPIAFGVRESLPASTLKQYVALAKTRPGKLSYGS
                     YGPGSGGHFVGELFNMAAGIDTVHVPYKGEPPALQDLIGGQIDAAVVSVGGVNRYPGK
                     IKPLAVASATRFPSYPDVPTFAELGYPEVDMPGWGAAFVPAKTPKAIVDKLNTEMGRI
                     LVMPDVRAKLLELGFEPAPWSPDKTRRFMKDQLALTQKLIDAGRVKL"
     gene            complement(5966..6691)
                     /locus_tag="PLL14_00480"
                     /pseudo
     CDS             complement(5966..6691)
                     /locus_tag="PLL14_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
     gene            complement(6688..7563)
                     /locus_tag="PLL14_00485"
     CDS             complement(6688..7563)
                     /locus_tag="PLL14_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOG02420.1"
                     /translation="MIDYEKTKAWRSDPVRHTYTERDTILYALGVGFAADPLVLEELR
                     FVYEKELQAVPTMAAVLASPGFWMRDRKELGIDYLKLVHGEQGVTIHSPLPAAGTLIG
                     QSRVTRIVDKGEGKGATMHVEKTLTDEASGRPVATAEMVAFLRGDGGFSKNGGGDEPV
                     PAAPATPDAAPEVTIEMPTRADAAVLYRLSGDINPLHIDPAVAAKAGFPRPILHGLAT
                     YGNACRGILAKFCGHDASRMKSIRARLTSPVYPGETLVLDCWRLGSEEIAFRASVKER
                     GVQVLANGRAVLGAR"
     gene            complement(7568..8365)
                     /locus_tag="PLL14_00490"
     CDS             complement(7568..8365)
                     /locus_tag="PLL14_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG02421.1"
                     /translation="MTGDSVLFERADGVAVLTLNRPSAKNAIDIPTTDALVAALRGLR
                     AADDIRAVVLAGAGGDFCAGGDVKGMADGASRTVEQRRAGMARYAELARALASLDKPL
                     IAAVDGVAFGAGLSMALYADIVLVSTRARLAMVFHRIGLVPDVGAWYQLPRVVGLQRA
                     KELIYSAREFGADEAVRMNIALEALAPEALMPRAMELARSLAGASPTAFSLSKRALAA
                     TLQSDLETMLDMEASSQAIALSSAYFREAIRRFAAKEPAQFCWPNKD"
     gene            complement(8367..9140)
                     /locus_tag="PLL14_00495"
     CDS             complement(8367..9140)
                     /locus_tag="PLL14_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG02422.1"
                     /translation="MDPIVSLQGRTIVVTGAGQGIGKGIVELAIELGANVVAVDLNGQ
                     TLDAAVSPLPQARVLAVQGSVADPAVADRAVTEAVAKFGAVHGLVNNAGIIRPAMIDK
                     MTDQQWQEVIDVHLTGSFYWMRATGRHMVERSKGGERTGGSIVNISSEAGRRGSIGQI
                     NYAAAKAGMLGMTMTAAREWGRYNIRTNSICFGVVETPMTEVVRGEKFRDGMLAQIPL
                     GRWSTPGEVVKSVCFLLSDGSSYITGQHLGVNGGIHISL"
     gene            9217..10386
                     /locus_tag="PLL14_00500"
     CDS             9217..10386
                     /locus_tag="PLL14_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOG02423.1"
                     /translation="MTIKRKACIAGAYEHPTRKAPDKTVAQLHAECARGALADAGLTL
                     QDVDGYFCAGDAPGLGANNMADYLGLTKLRHIDTTDTGGSAYLIHVSHAAQAIAAGKC
                     DVALVTLAGRPRSEGSSGVAPRLVTANTPDVPWEMPYSPVTVNMYALAAARHMHEYGT
                     TSEQLAWIKVAAAAHAQHNPNAMLREPVTVEEVLASPMISDPLHKLDCCVVSDGGGAL
                     VVVRPEIAARLKRPVVNILGAGETIKGQLGGHVDLTWSGAAISGPIAFKEAGVSPADI
                     QYASIYDSFTITVLMQLEDLGFCKKGEGGRFVADGNLISGVGTLPFNTDGGGLCNNHP
                     ANRGGITKVIEAVRQLRGEAHPKVQVPDCTLALAQGTGGLLGSRHGSATLIMERQ"
     gene            10390..10788
                     /locus_tag="PLL14_00505"
     CDS             10390..10788
                     /locus_tag="PLL14_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOG02424.1"
                     /translation="MTTPYTERPLAPVTVDAATEHFWTQAKEGLLCTRRCTSCGEVHW
                     YPRALCPFCMGDTEWCSLSGLGTIYSVSVTRRAGPIAYAIAYVALDEGITLLTNIVDC
                     DFDDLKIGQRVEVTFKPAEGGAMVPMFKPA"
     gene            10800..11597
                     /locus_tag="PLL14_00510"
     CDS             10800..11597
                     /locus_tag="PLL14_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG02425.1"
                     /translation="METLDFSVENAVGWIRLTRPEIHNPLDVPLRTDLMTVLEQVRND
                     PAVRVLVLNGGAGAFCAGGNLHALQEHANAGPAYWQRRINAGLRLTDDLLNVGCPVIA
                     AIDGPAIGAGFALALSADMIIASPRAKFAMAHLRLGLVPDLGALYLLPRVVGLQRAKE
                     LVFSTRDVSADEARELGIVMEVHASEALEARARDMAERMARAAPTAVALSKAMLNASL
                     DSDRQSAFALEAASQAAAFAAPEPAAQVEAILGKQRPAFSGLKYARG"
     gene            complement(11603..>12337)
                     /locus_tag="PLL14_00515"
     CDS             complement(11603..>12337)
                     /locus_tag="PLL14_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOG02426.1"
                     /translation="EAGFGAVGLRVSGRNPGDPWPQPNAGGDGLERIRARAEALSIRI
                     SSISGYYMSEKTSLSHLLANVEAARRVGAPLISQGCFEPDPSRVAELLRDYARAADDA
                     GVRIALEFMPMSSLKTIADAQRVIADSGARNVGLLIDSLHLARSGAGGADVRALDARC
                     IHLTQLCDAPAVRAAETTLFDEAMSGRMYVGDGGLDLKGLVEALSPDAEIELETPVIA
                     DATLPGGERARRAAEKAMAFFVSHFG"
BASE COUNT         2152 a         4219 c         3977 g         1989 t
ORIGIN      
        1 ccagtgctag cttttcgtac cgtcacttat tgcactgaaa acgaggagac cccggtacac
       61 ctcagacaac gcgtccacca tcatcagcac cagcgggttg tgcgtcgctt gcgcgatgag
      121 ccggtagaac tcgatcaccg actgcgtgtt gcgcgaggcg cgccccagtc ggaacagatc
      181 cgcatgcagg tcgatgtcgc gctggatcgt gtcgaactcc tcgttggtcg cgcgcgggca
      241 ggcgaggcgg atcgccacgc agatgatctc ggtgcgcgcc tccatcacgt tctccagcga
      301 cgcctgcgcg agcgacacca tgtcctgcac cgtctgcgca agaccgccgc tgccgccatt
      361 cctgatgaag aagccgccct ggatgccctt gcgcgcttcc accacgcccg agacctccag
      421 gctgcgcaat gcgtcgcgtg ctgccgcgcg tccgatgccg aagcgctcgg ccagttcgcg
      481 ctcgccgggg agctggtcgc ccggcttcag ctcgccgctc atcaactgtt cgcgaatgcg
      541 gtcgcacacg gcctcgaagc cacgccgggt gcgcacaggt tggaagagcg attcgttggt
      601 catgaaagat ccttttgagg gttcggcagt attgcatatt cattggttga actataagat
      661 gattcgccat ggacttggac ctcacccccg aacagcgcgc cttccgtgac gaggtgcgca
      721 ccttcatccg cgaaaagctg ccctcagaga tccgcgaacg cctgcgtgcc ggccatccgc
      781 cgcgcaagca ggacacagtg gcctggcagc gcatcctcaa cgagcgtggc tgggccgcgc
      841 cgcactggcc caagcgctac ggcggcgccg acctcggccc gatggaacgc ctgatcctgc
      901 tcgacgaagc ctatcgcgcc cccgccccgc tgccgcaggt gttcaacgtc ggcatgctcg
      961 gcccggtgct gatgaagttc ggcacgcctg cacagtgcga ccacttcctg ccgcgcctcg
     1021 cgaatctcga tatctggttc tgccagggct tctcggagcc cggctccggc tccgacctcg
     1081 cgtcgcttcg cactgccgca cgccgcgagg gcgatcacta catcgtcaac ggccagaaga
     1141 tctggaccac cactgcgcac ctcgccgact gggtgttcgc cctcgtgcgc accgataccg
     1201 aggcgcgcaa gcaggaaggc atttctttcc tgctgatcga cctgcgcacc cccggcatca
     1261 cgatccggcc gatccactcc atcgacggcg agcaccatct caacgaagtc ttcttcgacg
     1321 aggtgcgcgt gcccgttggc aacctggtcg gccaggagaa caagggctgg gattgcgcga
     1381 agttcctgct gtcgaacgaa cgcacgggca tcgccaacgt cggcctgtgc cgcgagcggc
     1441 tcgactacgc gcgcgaactc gccggacgct acacacaggc cggcaagccg ctgctcgacg
     1501 accccaagct gcgcgcagac atcgccatgc tggaggccga gacgcgcgcg ctggagatca
     1561 ccaactggcg cttcctgctc accgacagcg agcagaagcg cctgccggcc ttcgcgtcgg
     1621 tactcaagct caagggcacc gagttgcagc aggagatcaa cgcgcttctc gcacgcatcg
     1681 tcggcccggg cggtctggag cgccgctccg ccgacgaagt cacgcacggt ccgattgcgc
     1741 cgcgctattt ctattcgcgc gcagcgtcga tctacggcgg cacgaccgaa gtgcagaagg
     1801 acatcctggc caaggccatc gtcggataag gcaaacatga atttcgaact gaatgaggat
     1861 caggcggcgc tcgcggagag cctttcccgc ctgctggccg atcgctatgg attcgagaag
     1921 cgccgtgcgc tcaccgcctc cggcgcagcg catgacgaaa agacctgggc tggattggcc
     1981 gagctgggcc tgacagcgtt gccagtgcca gaagcactcg gcggcttcgg tggcggtgcg
     2041 cgcgacctgc tgcccgcgat gcaggccttc ggccgctcgc tctcgatgga gccgttcctg
     2101 gcgagcgcgg tgctcgcagg caccgccctg cgcctcggcg ccgacgaggc gatgcagaca
     2161 cgactgctgc ccggcatggc cagcggcgag atcgtgtcgg cctgggcgca tgacgaaccc
     2221 gccggccgtc acgcgccgct gtgggtggag acgcgtgccg aacgcaagga cggcggctgg
     2281 aagctcgacg gcatcaagtc caacgtgccc gccggaggtc tggcgcggta cttcgtcgtg
     2341 agcgcgcgca tcgccggtgc accggctgac gcgaacgggt gcgcgctgtt cctcgtggac
     2401 gccaaggacg caggcgtcgc gctgcgcgag ttccgcctgg tcgatgacac agcagcaggc
     2461 gagctggtgc tgacgcaagc agcggcgcag ccgttggcca accccgaaga ttcgactcgc
     2521 acgcgtctcg ccatccaagg caccgtggcc gctggcatcg ccgccgtgtg cgccgacatg
     2581 agcggcgccg cccaggccgc cttcgatctc gcgatggact acctgcgcac acgcaagcag
     2641 ttcggccgcc tgatcggcga gaaccaggcg ctgcgccatc gcgcgtccga gatgctggtc
     2701 agcctcgaga tggcgcgcag catggccatg gccgccgctg ttgcggcaga cgatgccagc
     2761 gcggaagaca gtgcccccga cctgcatcgc gcgaagctct ccgtcgcccg caacgcacgc
     2821 aacgtggcgc tcggcgcgat ccagcttcac ggcggcatcg gcatgacaga ggaatacgcc
     2881 gtcgggcact acctgcggcg catccacgtg ctcgaccaga tgttcggcga cgccgacgcc
     2941 catgccgcgc gtctcgcggc tgcccttctt tgagacctac accgatgagc atcaccccca
     3001 tgatctacct cgacggccgc ggcgtgacgc gcgacgaggt gctgcgcgtg gcgcagcagg
     3061 cagccacggg cttcgagcaa ctcggcgtga gcgaaggcga caccgtcgcc ctgctgctgc
     3121 gcaacgactt cgccttcttc gaagtacagc aggccgccgc cgcggtcggc gcctatggcg
     3181 tgccgctcaa ctggcacggc cgcacggaag aagtgatcta cgtgctcgaa gacgcgaagc
     3241 cgaaggtgct ggtcgcgcat gccgaccttc tcgccccgat ccgcgcgctc gtgcccgcgg
     3301 gcatccgcgt cttcgtggtg ccgacgccgc cggaaatcca gcagcgctac ggcgtctcga
     3361 ccgaactcgc acggccctta ccgggcgaca cggtttggcg cgaatgggcc gagcgcttcg
     3421 agccgctgca ggcgccgccg cgccgcgccc gcgcgaccat gatctacacc tccggcacca
     3481 ccggccatcc caagggcgtg aagcgccagc ccgccacgcc cgaggaaagc aaggcctatg
     3541 tcgagttgtt cgccagcgtg tatgggctca agcccggcat gcgtgcgctg gtgtgcggcc
     3601 cgctctacca cgcgtcgccc aacgggtacg gccgtcaggc gctgatgacg gcggacgtgc
     3661 tggtgctgca gtccaagttc gatccggaag agacgctggc gctgatcgag aagcaccgca
     3721 tcgacaacgc ggtgatggtg cccacgatgt tcgtgcgcat gctcaagctg ccgccggagg
     3781 tgcgcaaccg ctacgacgtg cgctcgctgc gatgggtgac gcacaccggc gcgccgtgtc
     3841 cgcgcgaggt gaagaaggat ctgatggatt ggtggggtcc cgtggtctac gagggctacg
     3901 gcggcactga agtcggtacc gccacgctcg ccacgccgca ggactggctg gagcatcccg
     3961 gttgcgtcgg catgcccacg cccggcacgc gcatggcctt ctatgacgag gagggaaatc
     4021 gcctgcccga aggcgagatc ggcgagatct ttgctcgcgt gccagcgtat gccgacttca
     4081 cttatctcaa ccacgaagag aagcgaaaga gcgtggagcg cgacggcctc atcagcctgg
     4141 gcgacgtggg ctacatgaag gaaggccggc tctatctctg tgaccgccgt tcggacatgg
     4201 tgatcttcgg cggcaccaac atctatccgg ccgagataga gatggtgctg acccagtgcc
     4261 ctggcgtgaa ggactgcgcc gtgttcggca ttcccgacga cgacttcggc gaatcgctcg
     4321 ctgccgctgt cgaacgcatg cccgagtcgc aagtcacgcc ggaggagatc cggtcctatc
     4381 ttctcgaacg tctcgcgaag ttcaaggtgc ccaagcggat cgacttccac gaatcgctgc
     4441 cgcgcgagga cagcggcaag atcttcaagc gccgcttgcg cgacccgttc tggcagaacg
     4501 ccgggcgcaa gatttgaggg gcggcgcatg ggcaagtggc tgcaatccaa tgaacacctc
     4561 accttcgagg tgcgagagcg cgtcgcgcgc atcaccctca accggccgga ccggcgcaat
     4621 gcgctgtcgt gggagatgct ctccgagctg cgcgcagcgc tgctggaggc cgacgacctg
     4681 cggagcgtga gcgtgatcgt gctcggaggc gcaggccccg atttctgcgc gggctacgac
     4741 atgaagtcgg cctacgcgcg ctacgccgcc gaaggcgatg ccgcgctcgc accctcagcg
     4801 cgccatcgaa ggcaccgatc tcgcggcgga tcaacgcatc gagcgcgctc gtctgcgccg
     4861 cgtcgagcgc acttgctgtc gtgaatgttg cggtggatga atttttcagt ttgtcgttta
     4921 atctatggtt gaaccaatga tttcgacgaa tcgtatggca gccagatcca tcacacaagg
     4981 agacatcgca atgaagttca ctcgacgcca gacgcttgct gcagccctgg gctcgctcgg
     5041 tgcgggcgtt ctgccgcgcg cgcaggcgca gacgcagccc ttcccttcac aggtcatcaa
     5101 gttcgtgatt cctacgccgg cgggcgggga ccacgacacc atgatgcgga tcattggcca
     5161 gaagctgacc gacgcctggg gccagccctg catcgtcgaa tccaggtccg gtgcgagcgg
     5221 cgccatcgcc gcagccacgg tgtcgcaggc gccggcggac ggatacaccg tgctgatcgg
     5281 ctacagcgcg cttctcagca acacggtgct gatgccgaag ccgggctatc ggctcgaaga
     5341 cctgcagccg gtcggaatgc tggctctcac gccgatcgcg ttcggcgttc gcgagagcct
     5401 gccggcctcg acgctcaagc aatacgtggc gctcgccaag acacggcccg gcaagctctc
     5461 ctatggctcc tatggtccag gctcgggtgg tcacttcgtc ggcgagctgt tcaacatggc
     5521 agccggcatc gacaccgtgc acgtgccgta caagggcgag ccgcccgcgt tgcaggacct
     5581 cattggcgga cagatcgacg cggcagtggt gtcggtcggc ggcgtgaacc gctaccccgg
     5641 caagatcaag cctcttgccg ttgccagcgc cacgcgcttt ccttcgtatc ccgacgtgcc
     5701 gaccttcgcc gaactcggct atccggaggt cgacatgccg ggctggggcg ccgccttcgt
     5761 gccggccaag acaccgaagg cgatcgtgga caagctcaac acggagatgg gccgcatcct
     5821 cgtgatgccg gatgtgcgcg cgaagctgct tgagctcggc ttcgagccgg caccgtggag
     5881 ccctgacaag acgcggcgct tcatgaagga ccagcttgcg ctcacgcaga agctgatcga
     5941 tgccgggcgc gtaaagctct agtcctcagc gcctgaccag caggcgcgcg atcaatcgtt
     6001 cgcgcgcttg ctgatcgaga ccgaaaggtc cttccaggta agcatgcatc gagccccagc
     6061 gcttgtcgat ttcatcgaat gcgcattgca gatagtcggc gtgcaccgtg gctcgcagtg
     6121 cccagtcggc ggaaacaccc tgcatggacc agcgaccgtt gctcgccata aagtcgtcga
     6181 gcacagtttc gcgcggcacg ccgagcgcaa gcagcagcaa cgccaaagcc cagccggtgc
     6241 gatcctttcc ggcggtgcag tgaaccacca acgggagcgg cgtcgcctca tcggcgacca
     6301 gccgcaggag cgaggcgaag acaggcgtgt gttcgttcac gaagcgccga tagacttcga
     6361 acatgatggc ggccagcgcg gacttgtcgg gtccacccgc ctcacgcagt tcgcgcagcg
     6421 cgcccatcgc gcgcggctcg atcggcagat gcggctcgcg gatgccgaat tgcgccaggc
     6481 gcgacggcgc acgggtccgc gcgtccgcgt cccgcagatc gatcaccgac tacagtccaa
     6541 tggcgcgcag cgcatccagt tcgtcgtccg agaccgaacc gaagtgactg gaacgccaga
     6601 gtcgaccggg tgcaatgaca tggcccgacg cggtggcgat gccacccagg tcgcgcaggt
     6661 tcggcacgcc ggcggcgacc agcccagtca tcgagccccc aggacagcgc gtccgttggc
     6721 cagcacctgg acgccgcgct ccttcacgct ggcacggaag gcgatctcct ccgagcccag
     6781 tcgccagcaa tcaagcacca gcgtttctcc gggatagacc ggcgaagtca accgcgcgcg
     6841 gatcgacttc atgcgcgacg catcgtgccc gcagaacttc gccaggatgc cgcggcatgc
     6901 gttgccatag gttgcgaggc catgcaggat cggtcgggga aatccggcct tggcagcaac
     6961 tgcaggatcg atgtgcagcg gattgatgtc gcccgagaga cggtacagca cggcggcatc
     7021 cgcgcgggtc ggcatttcga tcgtcacctc cggcgccgca tcgggtgtcg caggcgcagc
     7081 aggcacaggc tcgtccccac cgccgttctt cgagaaccca ccatcgccgc gcaagaaggc
     7141 caccatctcc gcggtggcga ccggccgtcc gctcgcttcg tcggtgaggg tcttctccac
     7201 atgcatcgtc gcgcccttgc cctcgccctt gtcgacgatg cgggtgacgc ggctctggcc
     7261 gatcagcgtg ccggctgcgg gcaacggcga atggatcgtc acgccctgtt cgccgtgcac
     7321 gagcttcagg tagtcgatgc cgagttcctt gcgatcacgc atccagaagc caggcgatgc
     7381 caggaccgct gccatcgtcg gtacggcctg gagttccttt tcgtagacga agcgcagctc
     7441 ttccaacacc agcggatcgg cggcgaaacc gacgccgagg gcatagagga tggtgtcgcg
     7501 ctcggtatag gtgtggcgca ccggatcgga acgccaggcc ttcgtcttct cgtagtcgat
     7561 catgctttca atccttgttc ggccagcaga actgcgccgg ctccttggca gcgaagcggc
     7621 ggatcgcttc gcggaagtac gcactggaca aggcaatcgc ctgcgatgac gcttccatgt
     7681 ccagcatcgt ctccagatcg ctctgcaagg tcgcggccag cgcgcgcttg gagaggctga
     7741 atgccgtcgg cgatgcaccc gcgagactgc gggccagttc cattgcgcgc ggcatcaggg
     7801 cctccggcgc gagagcctcc agcgcgatgt tcatgcgcac cgcctcgtcg gcaccgaact
     7861 cgcgggccga atagatcagt tccttggcac gctgcaaccc caccacacgt ggcaactggt
     7921 accaggcacc tacatcgggc acgaggccga tgcgatggaa caccatggct aggcgcgcgc
     7981 gcgtgctcac cagaacgatg tcggcataga gcgccatgct gaggcccgcg ccaaaagcga
     8041 cgccgtcgac tgcggcgatc aacggcttgt ccagcgacgc cagcgcgcga gccagttcgg
     8101 catagcgcgc catgcccgcg cggcgctgct cgaccgtccg cgaggcgcca tcggccatgc
     8161 ccttgacgtc gccgcccgcg cagaagtcgc cgcccgcacc cgccaacacc acggcgcgga
     8221 tgtcgtcggc ggcacgcagc ccacgcaagg ctgcgacgag cgcgtcggtg gtcgggatat
     8281 cgatcgcgtt ctttgccgat ggccggttca gcgtgaggac ggccacgccg tccgcgcgct
     8341 cgaagaggac gctgtcgccc gtcatgtcag aggctgatgt ggatgccgcc gttcacaccc
     8401 agatgctggc ccgtgatgta actcgatcca tccgacagaa ggaagcagac gctcttcacc
     8461 acctcgcccg gcgtggacca gcgacccaac ggaatctgag cgagcatccc gtcgcggaac
     8521 ttctcgccgc gcacgacttc ggtcatcggc gtttcgacca cgccgaagca gatcgagttg
     8581 gtgcggatgt tgtagcgacc ccattcgcgt gcggcggtca tcgtcatgcc cagcatgccg
     8641 gccttggctg ctgcgtagtt gatctggccg atcgagcccc tgcgcccggc ctcggacgag
     8701 atgttgacga tcgagccgcc cgtcctttcg ccgcccttgg accgctcgac catgtgacgg
     8761 ccggtggcac gcatccagta gaaagaaccg gtgaggtgca cgtcgatcac ctcctgccat
     8821 tgctggtcgg tcatcttgtc gatcattgcg gggcggatga tgccggcgtt gttcacgagg
     8881 ccgtgcacag cgccgaactt ggcaaccgct tcggtaaccg cacgatcggc cacagccggg
     8941 tcggccacgc tgccttgcac tgcaagcacg cgcgcctgcg gcagcgggga gaccgcggcg
     9001 tcgagcgtct gaccgttgag gtcgacggcc accacgttgg cgccgagttc gatggccagt
     9061 tccacaatgc ccttgccgat gccttggccg gccccggtga cgacgatggt gcgcccttgc
     9121 aggctgacga tgggatccac tttgatgctc cttgtttgct tgttcgatgg ttgaaccaat
     9181 gatatcctac tcgcgaacag tggctggagc aaacacatga ccatcaaacg caaggcctgc
     9241 atcgcaggcg cctatgaaca cccgacccgc aaggcccccg acaagacagt ggcgcaactg
     9301 cacgctgaat gcgcgcgcgg cgcgctggcg gatgccggac tcaccctaca ggacgtcgac
     9361 ggctacttct gcgctggcga tgcgcccggc ctgggtgcca acaacatggc cgactatctg
     9421 ggcctgacca agctgcgcca tatagacacc accgacaccg gcggttcggc ctacctgatc
     9481 cacgtgtcgc acgcggcgca ggcgatcgcc gccggcaagt gcgatgtcgc gctggtgacc
     9541 ctcgcgggtc gtccgcgcag cgaaggatcg agcggcgtcg cgccccggct cgtcacggcc
     9601 aacacgccgg acgtgccctg ggagatgccc tacagccccg tcaccgtgaa catgtacgcg
     9661 ctggctgccg cgcgccacat gcacgagtac ggcaccacca gcgagcaact cgcgtggatc
     9721 aaggtggcag ccgctgcgca tgcgcagcac aacccgaacg cgatgctgcg cgaaccggtg
     9781 acggtggaag aagtgctggc ctccccgatg atttccgatc cactgcacaa gctggactgc
     9841 tgcgtggtga gcgacggcgg cggcgcgctg gtggtggtgc ggcccgagat cgccgcgcgc
     9901 ctgaagcgcc cggtggtcaa catcctcggc gcgggcgaaa ccatcaaggg tcagctcggc
     9961 ggccacgtcg acctgacctg gtccggcgca gcgatctccg ggccgatcgc gttcaaggaa
    10021 gcgggcgtga gcccggccga catccagtac gcatcgatct acgacagctt caccatcacc
    10081 gtgctgatgc agctggaaga cctcggcttc tgcaagaagg gtgaaggcgg tcgcttcgtg
    10141 gccgacggca acctgatctc gggcgtcggc acgctgcctt tcaataccga cggtggcggg
    10201 ctgtgcaaca accatccggc caatcgcggc ggcatcacga aggtgatcga ggccgtgcgc
    10261 cagttgcgcg gcgaagcaca cccgaaggtc caggtgcccg actgcaccct ggctctcgcc
    10321 caaggcaccg gcggcctgct cggctcgcgc cacggcagcg ccaccctgat catggaaagg
    10381 caataagcga tgaccactcc ctacaccgag cgcccactgg cacccgtgac cgtcgacgcc
    10441 gccaccgagc acttctggac gcaggccaag gaaggccttc tttgcacgcg gcgctgcacc
    10501 agttgcggcg aggtccactg gtatccgcgc gcgctctgcc ccttctgcat gggcgacacc
    10561 gagtggtgca gcctctcggg actgggcacg atctacagcg tgagcgtgac gcgacgcgcc
    10621 gggcccattg cctacgcgat cgcctacgtc gcgctcgatg aaggcatcac cctgctcacc
    10681 aacatcgtcg actgcgattt cgatgacctg aagatcgggc aacgcgtcga ggtgaccttc
    10741 aagccggcag agggcggcgc gatggtcccg atgttcaagc cggcctgagc ccagtgcaga
    10801 tggagacgct cgatttctct gtggagaacg cggtcggctg gattcgcctc acccggccgg
    10861 agatccacaa cccgctggac gtgccgctgc gcaccgacct catgacggtg ctggagcaag
    10921 tgcgcaacga ccctgcggtg cgcgtactcg tcctcaacgg cggcgcgggc gccttctgcg
    10981 ccgggggcaa cctccacgcg ctgcaggagc atgcgaatgc agggcccgcc tactggcaac
    11041 ggcggatcaa cgccggtctt cgcctcaccg acgatctgct gaacgtgggc tgccccgtga
    11101 tcgccgccat cgacggtccg gcgatcggcg ccggtttcgc gctggcgctc tccgcggaca
    11161 tgatcatcgc cagcccgcgc gcgaaattcg ccatggccca cttgcggctc ggactcgtgc
    11221 cggaccttgg cgcgctctat ctgctgccgc gtgttgtggg gctccagcgt gcgaaggagc
    11281 tggtgttctc cacgcgcgat gtcagcgccg acgaggctcg cgaactcggc atcgtgatgg
    11341 aggtgcacgc cagcgaagcg ctcgaagcac gtgcgcgcga catggccgaa cgcatggccc
    11401 gggccgcgcc gaccgcagtc gcgctttcca aggcaatgtt gaatgcctcg ctcgatagcg
    11461 acagacagag cgccttcgcg ctggaagccg ccagccaggc tgccgctttc gcggcgccgg
    11521 aaccggccgc gcaggttgaa gcaattcttg gaaaacagcg tccggccttc agcggcctga
    11581 agtacgcgcg aggctgacga cctcagccga agtgcgatac gaagaaggcc atggccttct
    11641 ccgctgcacg tcgcgcgcgt tcgcctcctg gcagcgtggc gtcggcaatg acgggcgttt
    11701 ccaactcgat ctccgcgtcc ggcgacaagg cttccacaag ccctttcaga tcgagccccc
    11761 catcgcccac gtacatgcgg cccgacatcg cttcgtcgaa cagggtcgtt tcggccgccc
    11821 gcacggcggg cgcatcacag agttgcgtga gatggatgca tcgggcgtcg agcgcacgca
    11881 cgtccgcgcc gccggcgccc gagcgcgcga gatggagcga gtcgatcagc aggcccacgt
    11941 tgcgcgcacc gctgtcggca atcacgcgct gcgcatcggc aatcgtcttg agcgagctca
    12001 tgggcatgaa ctcgagcgca atgcgcacgc cggcatcgtc ggccgcgcgc gcgtagtcgc
    12061 gcagcagttc ggccacgcgc gacggatcag gctcgaagca tccctgcgag atcagcggcg
    12121 caccgacgcg gcgtgcggct tcgacgttgg cgagcaggtg cgacaggctc gtcttctccg
    12181 acatgtagta gccggagatg cttgagatgc ggatcgagag cgcctcggct cgcgcgcgta
    12241 tccgttccag gccgtcaccc ccggcattcg gctgcggcca cggatcaccg ggattgcgac
    12301 cgctgacacg caagcccaca gcgccgaagc ctgcttc
//
LOCUS       DAOSIT010000008        12305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_5175_length_12305_cov_7.713633, whole genome shotgun sequence.
ACCESSION   DAOSIT010000008 DAOSIT010000000
VERSION     DAOSIT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_12305_cov_7.713633"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..>1357)
                     /locus_tag="PLL14_00520"
     CDS             complement(285..>1357)
                     /locus_tag="PLL14_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02427.1"
                     /translation="GIPPKKGNLMPTGIAADLRSIAEAAVMAPSADNQHCFELRARDD
                     RILLSGSEAYVGAPFHKKLLSLISFGAVVENMTIRAARLGYQTSVLWLPDPLRQPVIA
                     ELQLARGAPSAPPLDTAISRRHTNRRIRFGGPPLSDSERSEFQHLLRPIDGVTLDFFD
                     RGRQHSELLHLIRIAEAERFNSQALHADLFSAVRFDVGWHASADVGLPPAALAVEPGA
                     RWAFTQLRRWPVMNGLRRFGVQHALGFRAGYLPCRLAPQLCVLSTSLPLDQGAPAVGR
                     ALERFWLEAETRGLALQPFAGSALLALKEYPDVPPATGEALRRGWKHLVGAQPLMVFR
                     LGRARRPLIRTSRQAPEHFLRD"
     assembly_gap    1358..1457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2093..3034
                     /locus_tag="PLL14_00525"
     CDS             2093..3034
                     /locus_tag="PLL14_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOG02428.1"
                     /translation="MENLIGPQGVTLLSDQPLPGRGASSWSYGEAFSRNLGLVSAAEQ
                     ERLRASRVAVVGLGGVGGVDLVALARLGIGKFSIADPDIFEMRNTNRQYGATRSAEGR
                     YKADVMCQIVRDINPEADVRVFREPIGPLNADAFLEGADVLVDGIDAFEIDLRRLLFR
                     KARERGIHALGAGPVGFSTVWVIFGPQGMSFDRYFDLADQMDGVETFVAYVTGMAPSS
                     LQRGYMDLSYLNFEGHTGPSAGLACHLASGVIAAEVLKILLARGRIYTAPVYHQFDAY
                     LGRFVRKRLFGGNRNPLQRIKRRWLVNYIRQRLKSKA"
     gene            3148..4206
                     /locus_tag="PLL14_00530"
     CDS             3148..4206
                     /locus_tag="PLL14_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOG02429.1"
                     /translation="MPATRFVEGVLLRRDIERLYLVFGGHIFFQTLRTAVQLDLFTLL
                     SREGPLTRQEIAKRLGVAEQPARVMILGLTVVGLLRKSGAKYTNSRLAGTLLVKDSPR
                     KITAYVELQHRAMYKGLYWMLEAVREYRNVGIKEFAGEEATLYERLAHDPEVEQIFQE
                     AMQELSLQANADMARFIDLRGVKHLVDVGGGDGTNIIAIARRWPHLRATVFDSPTVCE
                     IARKNIAACGLADRLDAVPGECFADPFPQDADCLVFAHFFTIWGEKQDRELLKKCYDS
                     LPSGGQVVIFNMMQHDDESGPFSAAVGSPYFLTLATGVGMLYTWNEYETWMRDAGFND
                     VRRHRLLRDHGLIVGRKP"
     gene            complement(4307..5371)
                     /gene="fba"
                     /locus_tag="PLL14_00535"
     CDS             complement(4307..5371)
                     /gene="fba"
                     /locus_tag="PLL14_00535"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOG02430.1"
                     /translation="MPIVSMRQLLDHAAENGYGLPAFNVNNMEQVWAIMEAAAEVDAP
                     VIMQASAGARKYAGEPFLRHQILAALEAYPQIPIVMHQDHGQSPAVCMSAIRSGFSSV
                     MMDGSLMADGKSVASYEYNVEVTRKVVDFAHAIGVSVEGELGVLGSLETMRGDKEDGH
                     GADGQMTREQLLTDVEQAADFVRLTQCDALAIAIGTSHGAYKFTKKPTGDILAIERIK
                     EIHARIPNCHLVMHGSSSVPQELLAEIRQFGGDMKETYGVPVEEIVIGIKHGVRKVNI
                     DTDIRLAMTGAIRRYFAENPSKFDPRDYLKPAREAARKICRARYEAFGTAGQASRIRV
                     IPLDKMAERYSQGELTQTVL"
     gene            complement(5434..6867)
                     /gene="pyk"
                     /locus_tag="PLL14_00540"
     CDS             complement(5434..6867)
                     /gene="pyk"
                     /locus_tag="PLL14_00540"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG02431.1"
                     /translation="MSRDTKIVATLGPASSDAEVLERMIRAGVDVVRLNFSHGKPQDH
                     VDRARLVREVAARVGRPVGILADLQGPKIRVGKFADGKVRLETGERFILDARCELGNN
                     ERVGLDYKDLTRDVSAGSVLLLDDGRIVLDVQRVVASEIFTVVRHGGDLSDNKGINRQ
                     GGGLSAPALTAKDMEDIRTAASIDADFIAVSFPKSAADMYMAKQLIHAAGGQAQTIAK
                     IERVEAVAALEEIISASDGIMVARGDLAVEVGDAAVPALQKRMIRLAREKNKLAITAT
                     QMMESMILSPAPTRAEVSDVANAVLDGTDAVMLSAETASGRYPVETVEAMARICEEAE
                     KSAAVTLDHEFLNQVFTRIDQTISLAAIWTAHHLKVKAIAALTESGATALWMSRLNCG
                     VPVYALTPQAEAVTKMSLYRAVFPLFMKQRPTTRDELLYDAEQLLMNEGIVVKGDFIV
                     LTAGDPMGTSGGTNTLKIVRVGDQQPC"
     gene            complement(6922..7920)
                     /gene="gap"
                     /locus_tag="PLL14_00545"
     CDS             complement(6922..7920)
                     /gene="gap"
                     /locus_tag="PLL14_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG02432.1"
                     /translation="MTIKVGINGFGRIGRMVFRAAVQHFDDIEVVGINDLLEPDYLAY
                     MLKYDSVHGRFKGTIAVEGNTLIVNGKKIRLTAVKDPAELKWDEVGADIVVDSTGLFL
                     TTESCQKHIAAGARKVIQSAPSKDDTPMFVFGVNDHTYAGQTIISNASCTTNCLAPVA
                     KVLHDNWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTGMAFRVPTSDVSVVDLTVELNKDATYEDICAAMKAASEGPMKGVLGYT
                     DEKVVATDFRDEVCTSVFDAEAGMALDSTFVKVVSWYDNEWGYSCKVLEMVRVMAR"
     gene            complement(8152..8448)
                     /locus_tag="PLL14_00550"
                     /pseudo
     CDS             complement(8152..8448)
                     /locus_tag="PLL14_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002657.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
     gene            8416..8613
                     /locus_tag="PLL14_00555"
     CDS             8416..8613
                     /locus_tag="PLL14_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02433.1"
                     /translation="MNEVDLLDRVHGQGADGAGQGGKSSGHDDSPIQEMGFSRMCKLS
                     AKTGGFANFRSDAGFGIAAGH"
     gene            8773..10152
                     /locus_tag="PLL14_00560"
     CDS             8773..10152
                     /locus_tag="PLL14_00560"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HOG02434.1"
                     /translation="MDQSNRYADLSLREEDLIKGGRHILVAYKMKPKAGYDYLQAAAH
                     FAAESSTGTNVEVCTTDEFTRGVDALVYLIDEAGEEMRIAYPLDLFDRNVTDGRMMIV
                     SFLTLVIGNNQGMGDIEHAKIYDFYMPERAIQLFDGPAKDISDLWRILGRPVKDGGYI
                     AGTIIKPKLGLRPEPFAKAAYQFWLGGDFIKNDEPQGNQVFAPMKKVMPLVYDAMKRA
                     QDETGQAKLFSANITADDHYEMCARADYILETFGPDADKVAFLVDGYVGGPGMVTTAR
                     RQYPNQYLHYHRAGHGAVTSPSSKRGYDAYVLAKMSRLQGASGIHVGTMGYGKMEGSA
                     DDRAIAYVIERDEYQGPAYHQKWYGMKPTTPIISGGMNALRLPGFFANLGHGNVINTS
                     GGGSYGHIDSPAAGAISLRQAYECWKAGADPIEWARQHPEFARAFESFPGDADKLYPG
                     WREKLGVHR"
     gene            10241..11044
                     /locus_tag="PLL14_00565"
     CDS             10241..11044
                     /locus_tag="PLL14_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOG02435.1"
                     /translation="MTDKGQFRVLDEPYYQPQGNEVALYEAAYAARLPVMVKGPTGCG
                     KSRFIEYMAWRLGKPLITIACNEDMTASDLVGRYLLEANGTRWLDGPLTTAARIGAIC
                     YLDEIVEARQDTTVVIHPLTDHRRTLPLDKKGELLRAHPDFQLAISYNPGYQSLMKDL
                     KQSTKQRFTAFEFEYPAAALEIAILAGESGIDTVTAAQLVNVGAAARRLKGHGLDEGI
                     STRLLVYAATLIRAGVSPREACRMALVRPITDDRDIRDTLDHAIDATFA"
     gene            11066..>12305
                     /locus_tag="PLL14_00570"
     CDS             11066..>12305
                     /locus_tag="PLL14_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02436.1"
                     /translation="MSANVTDSRDPATWDERLQGYRAQLDCRFPQVAEVFERCLIEAL
                     AVLSTEGVDAYLDHARVLSRMGRGVEPVLVFLEEWPSIAAILGEQALPSIARTIDALG
                     KSPNAKAIFPFLQTLAAVARRLPAAAQMQHYLDLALDLMQRTSGSIHGIHQTFASPAL
                     PEFFGQAPRLLHALSIAGLSKWVEYGIRNYHNHPERQRAYFSLQSPDSRAVLQRERHG
                     TLLVDHERQLDLYLRGLWGDSALLVPYATNLEQSPQSVPYYDASGIRLPDVFDDAHGV
                     SGIDRYRALLAHIAGHRRWSRPIFADNFSPMQRLAIEVFEDSRIEELILRAYPGLRHI
                     FLALHPIPLEGACDPETSSCLRHRLTMLSRALLDPEHGYQDVPLLEFVDRFRETMARG
                     ESGSEEMAALALAYVARTRRQ"
BASE COUNT         2303 a         3661 c         3800 g         2441 t
ORIGIN      
        1 ggtcaaggcc tgtcgaacgg cgccatcgct cacagcgatc tggatgctca gaagtatgtc
       61 gctcccgagc cgggttccct ggcgctcctg ggcgccggcc tgttcggcct gcagggtctg
      121 cgtcgccgtc aagccaagaa gacggtctga agcagcgaag agattcagtc ttacggatcg
      181 aaaacccgcc actccggcgg gtttttttta tgttcgattc gcacgccccc caggcagggg
      241 ccgtcgagtg taccgagtcc gatggtgtcg tgttgcggcg gccgtcagtc gcgaagaaag
      301 tgttcaggcg cctgccggct ggtccggatc agaggccgtc tggctctccc cagacgaaag
      361 accatcagcg gctgcgcgcc gacaagatgc ttccagccgc gacgcagcgc ctcgccggtt
      421 gccggcggca catccggata ctccttcagc gccagcaggg cggatccggc aaaaggctgc
      481 agcgccaggc cgcgcgtctc cgcctcgagc cagaagcgtt cgagggccct gccgaccgcg
      541 ggtgcgcctt ggtccagtgg caggctggtg ctcagcacgc agagctgggg ggcgagacgg
      601 catggcaggt agcccgctcg gaatcccaat gcgtgctgca ctccgaatcg ccgcaagcca
      661 ttcatgaccg gccagcgcct gagctgggta aacgcccagc gggcccccgg ttccacggcc
      721 aacgcggccg gaggcagacc gacgtccgcc gacgcgtgcc agccgacgtc aaagcgcacg
      781 gccgaaaaca ggtccgcgtg cagggcttgg ctattgaagc gctccgcttc ggcgattctg
      841 atgaggtgca gtaactcgct gtgttgcctg ccgcgatcga agaaatccag cgtcacgccg
      901 tcgatgggac gcaacagatg ctggaactcg ctgcgctcgc tgtcgctgag tggcggtccg
      961 ccaaaccgga ttcggcgatt ggtgtgccgc cttgagatgg cggtatcgag agggggggcg
     1021 ctcggtgcgc cgcgcgccag ctgcaactcg gcgatcactg gctgccgcaa ggggtcgggt
     1081 agccagagaa cgctcgtttg gtatcccagg cgcgctgcac gaatggtcat gttttcgacc
     1141 accgcgccga acgagatcag gctcagcagc ttcttgtgga atggcgcgcc gacatacgct
     1201 tcgctgccgg agagcaggat gcggtcgtct ctcgcccgca actcgaaaca gtgctggtta
     1261 tcggccgaag gcgccattac tgcggcctca gcgatgcttc gcagatcggc agcaatcccg
     1321 gtgggcatga gatttccctt cttgggtggt attcccgnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnaga tcggcagcaa tcccggtggg catgagattt cccttcttgg
     1501 gtggtattcc cgctgcgccg gcgcacatgt ccttgacccc tgtcgccagg gcgatgagcg
     1561 aggaattgat ggatggtcgt cgactcgacc agccgcgact tcaataacat ctgccatgtc
     1621 atcgagcttg ccagatccgg cgccaagtcg attcttggta acgttcgagc gacattaaca
     1681 aatagcccca tccagtacaa gtcaaaggga gtgccgacag gaggtggctg gctgcgaggc
     1741 gatgcctttc ccaggcttgc cggggtagca ccgcaatgcg ctccgcgttg aagatctcac
     1801 acttgtgcta tggttgcagc cgtcgaaagg cagaagaggg atgcagcgtt cggcccggtc
     1861 atcgcaaaca tcgccagtgc aggtgctgag agccgctggg agttatggcc gtgtcactgg
     1921 tatgcgcccg accagcagca tttgccggcc ggcccacgct gggtggtacg gttagcgctg
     1981 aaaggcggta gaatccaatc gccgaaatga aacaacaggg cctgaatctg caggaatcag
     2041 tccggcttct tcctgcgcaa taacggcttg actgtccgct agggttggtg gcatggaaaa
     2101 cttgattgga ccacagggag taacgttgct ttccgaccag ccgctgccag gtcgaggagc
     2161 gtcgtcgtgg tcctatggcg aagccttttc acgcaatctg ggactggtca gtgctgccga
     2221 gcaggagcgc ctgcgggcga gccgtgtggc ggttgtcggt ttgggagggg tcggtggcgt
     2281 cgatctggtt gccctggccc gcttggggat cggcaaattc agcattgccg atcccgacat
     2341 ctttgaaatg cgcaatacca accggcaata tggcgcaaca cgttcggcgg aaggccgcta
     2401 caaggccgac gtcatgtgcc agatcgtgcg agacatcaat cccgaggcgg atgtccgcgt
     2461 ttttcgcgaa cccatcgggc cgttgaacgc ggacgccttt ctcgaaggcg ctgacgtact
     2521 ggtggatggg atagacgcct tcgagattga tctgcgtcgc ctgttgttcc gcaaggcccg
     2581 cgaacgtggc atccatgcgc taggcgcggg gcctgtcggc ttcagtacgg tatgggtgat
     2641 attcgggccc cagggcatga gtttcgatcg ctacttcgat cttgccgacc agatggacgg
     2701 cgttgagacc tttgtcgctt acgtcacagg aatggctccc tcgtctctgc agcgcggcta
     2761 catggatctg tcttacctga acttcgaggg gcacaccgga ccctcggcag gcctggcctg
     2821 ccatctggcg tcgggggtca ttgccgccga ggttctgaaa atcctcctgg cgcgcggccg
     2881 aatttacacg gcaccggtat accatcaatt tgatgcgtat ctcggtcgtt ttgtccgcaa
     2941 acgcctgttc ggagggaatc gcaaccctct gcagaggatc aagcgtcgct ggttggtcaa
     3001 ctacatcaga cagcgcctca agtccaaagc ataagggcgt gccaacgcaa tcttcaacct
     3061 cgatataggg gcacatttca aatggcaaat tcacacggct ctggcccaca aagcgcagtc
     3121 ggtaggctct gggatcaata cgtggcgttg cccgccacac gatttgtcga gggcgttctg
     3181 ctccggcgag acatcgagcg cctgtatctg gtcttcggtg gtcacatctt ctttcaaacc
     3241 ttgcgtacgg ccgtgcaact ggacctcttt acgctgctgt ccagggaggg gccgctcacc
     3301 cgccaggaga ttgccaagcg tcttggcgtt gccgaacagc cggcaagggt catgatcctg
     3361 gggctgacgg tggtcgggct actccgcaag agtggtgcaa agtacaccaa cagtcgcctg
     3421 gcgggaacct tgctcgtcaa ggatagtccg cgcaagatca cggcctacgt cgagttgcag
     3481 caccgggcca tgtacaaggg gctgtactgg atgctggagg ccgttcgcga gtaccgcaac
     3541 gtggggatca aggaatttgc cggcgaggaa gcgacacttt atgaacgcct ggcgcatgat
     3601 ccggaagtcg agcagatttt tcaggaagcc atgcaggaac tgtcgctgca ggccaacgcc
     3661 gacatggcgc gcttcatcga cctgcgtggg gtgaaacatc tggtggacgt gggcggtggc
     3721 gatggcacca acatcattgc cattgcgcgt cgttggccgc acctgcgggc aacggttttc
     3781 gactcgccaa cggtgtgtga aatcgcccgc aagaatattg ccgcttgcgg acttgccgac
     3841 cgcctggatg cggttcccgg agaatgcttt gccgacccgt tcccgcagga tgcagactgc
     3901 ctggtgttcg cacacttttt cacgatctgg ggcgagaagc aggaccgcga actgttgaag
     3961 aaatgctacg actcgctgcc atcgggcggt caggtcgtga ttttcaacat gatgcagcat
     4021 gacgacgaga gcggcccgtt cagtgctgct gtcggatctc cgtattttct cacccttgcc
     4081 acaggggtgg ggatgctcta cacgtggaac gagtatgaga cgtggatgcg cgacgccggc
     4141 ttcaatgatg tacgtcggca ccgcttgctg cgggatcacg ggctgatcgt cggcaggaaa
     4201 ccgtaaacgg cacttccagc gagtcttcag acacggcgct gcgcgccatc cccaccatcc
     4261 ttggcaagaa acggaacacg ccagcggcgt gttccgttgc cggcgatcag aggacggtct
     4321 gggtcaactc gccctggctg tagcgttcgg ccattttgtc cagcgggatg accctgatcc
     4381 ggctggcctg gccggcggta ccgaaagcct cgtagcgggc ccgacaaatc ttcctggccg
     4441 cttcacgggc tggtttgagg tagtcgcgcg ggtcgaactt ggacgggttt tcggcaaagt
     4501 agcggcgaat ggcgccagtc attgccaggc ggatgtcggt gtcgatattg accttgcgta
     4561 cgccgtgctt gatgccgata acgatttcct cgaccggtac gccgtaggtt tccttcatgt
     4621 cgccgccgaa ctggcgaatt tccgcaagca gttcctgcgg caccgacgaa gaaccgtgca
     4681 tgaccaggtg gcaattcggg atgcgcgcgt ggatttcctt gatccgctcg atggcgagaa
     4741 tgtcgccggt gggcttcttg gtgaacttgt aggcgccgtg cgacgtgccg atggcgatcg
     4801 ccagggcgtc gcattgcgtc agtctgacga agtcggcagc ctgctcgaca tcggtcagca
     4861 actgctcgcg ggtcatctga ccatcggcgc catggccatc ctccttgtca ccgcgcatgg
     4921 tttccagtga accgagaacg ccgagttcgc cttcgaccga gacaccgatg gcgtgcgcga
     4981 agtcgaccac cttgcgagtg acctcgacgt tgtattcata agacgccacg ctcttgccgt
     5041 cggccatcaa cgagccgtcc atcatcaccg aggaaaaacc tgaacggatc gcgctcatgc
     5101 acacggccgg ggactggccg tggtcctggt gcatgacaat cgggatctgc gggtaggcct
     5161 ccagcgccgc caggatctgg tgacgcagaa acggctcacc ggcgtacttg cgggcaccgg
     5221 cagaggcctg catgatcacc ggcgcatcga cttcagcggc ggcttccatg atcgcccaga
     5281 cctgttccat gttgttgacg ttgaaagcgg gcagaccata gccgttctcg gcggcgtggt
     5341 cgagcaattg acgcatcgat acgatgggca tgatgtacct tctccagtga gggctgcttg
     5401 cttgagtgaa ggagcgtgca cgttcgctga ccgtcagcag ggctgctgat ctccgacacg
     5461 cacgatcttc agcgtattgg tgccgccgct ggtgcccatc gggtcgccgg cggtgagcac
     5521 gatgaaatct cccttgacga cgattccctc gttcatcagc agttgttcag catcgtagag
     5581 cagttcgtcg cgggtggtcg ggcgctgttt catgaacagt gggaacaccg cccgatagag
     5641 gctcatcttg gttaccgctt cggcctgtgg cgtcagcgca tacaccggaa cgccgcagtt
     5701 gaggcggctc atccacaggg cggtcgcccc tgattcggtg agtgccgcga tggctttgac
     5761 cttgaggtgg tgcgccgtcc atatcgcggc cagcgagatg gtctgatcga tgcgcgtgaa
     5821 cacctgattg aggaactcgt ggtcgagcgt caccgccgcc gacttttcgg cttcctcgca
     5881 gatccttgcc attgcctcga cggtttcgac cggatacctg ccggaggcgg tttcggccga
     5941 cagcatcacg gcgtcggtac cgtcgagcac cgcgttggcc acgtcggaga cttcggcgcg
     6001 cgttggtgcc ggtgagagaa tcatcgattc catcatctgc gtggcggtga tcgccagttt
     6061 gtttttctcg cgtgccaggc ggatcatccg cttctgcaag gccggtacgg cggcatcgcc
     6121 gacctcgaca gccaggtcgc cgcgcgcgac catgatgccg tcggaagcgg agatgatctc
     6181 ttccagggcg gccacggcct cgacacgttc gatcttggcg atggtctgcg cctgtccacc
     6241 ggccgcgtga atcagttgtt tggccatgta catgtcggcc gcgctcttgg ggaaggaaac
     6301 ggcaataaaa tcggcgtcga tgctggccgc ggtccggatg tcttccatgt ccttggcggt
     6361 cagcgccggt gccgaaagcc cgccgccctg ccggttgatg cccttgttgt ctgacagatc
     6421 gccaccgtgg cgaacgacgg taaagatttc cgacgccacg acccgctgta cgtcaagcac
     6481 gatgcgaccg tcgtcgagca gcaggacact gccggcggag acgtcccggg tcagatcctt
     6541 gtagtcgagg ccgacacgtt cgttgttgcc cagttcgcag cgggcatcga ggatgaagcg
     6601 ctcgccggtt tcaagccgaa ccttgccgtc ggcgaatttc ccgacgcgga ttttcgggcc
     6661 ctggagatcg gcgagaatgc ccaccgggcg cccgacgcgg gcagccacct cgcgcaccag
     6721 tcgtgccctg tcgacgtggt cctgcggttt gccatgggag aagttgagcc gcacgacatc
     6781 gacgccggcg cgaatcatcc gttccagcac ctcggcgtcc gatgacgccg gcccgagggt
     6841 ggcgacgatc ttggtatctc gtgacatgaa tttcctatgc gagaacacga ggggcgcccg
     6901 taagcgcccc tctgtggccg tctagcgggc cattacacgg accatttcca gcaccttgca
     6961 ggagtagccc cactcgttgt cgtaccacga gacgaccttg acgaacgtgc tgtcgagggc
     7021 catcccggct tctgcgtcaa aaacggaagt gcagacttcg tcacggaaat cggtggcgac
     7081 caccttctca tccgtatatc cgaggacgcc cttcatcgga ccttcggaag cggccttcat
     7141 ggctgcgcag atgtcctcgt aggtggcatc cttgttcagt tcgacggtaa ggtcgacgac
     7201 ggacacgtcg gaagtgggga cgcggaaggc catgccggtg agcttcttgt tcaattccgg
     7261 aatgaccttg ccgacagcct tggcggcacc ggtcgaggac ggaatgatgt tctcgagaat
     7321 gcctcggccg ccacgccagt ccttgttgga tggaccgtcg acagtcttct gggtggccgt
     7381 tgcggcatgc accgtggtca tcagcccgcg cttgattccc caattgtcat gcaggacctt
     7441 ggccaccggt gccagacagt tggtggtgca cgaggcgttc gagatgatcg tctggccagc
     7501 gtaggtgtga tcattcacac cgaatacgaa catcggtgtg tcgtccttgc tcggagcgct
     7561 ctggatgact ttccgggcgc ctgcggcgat atgcttctgg cacgattcgg tggtcaggaa
     7621 gaggccggtg gagtcgacga cgatgtcggc gccgacttca tcccacttga gttctgccgg
     7681 atccttgacg gcggtcaggc ggatcttctt gccattgacg atcagcgtgt tgccctcgac
     7741 ggcgatggtg cccttgaagc ggccatgcac cgagtcatac ttgagcatgt acgcgagata
     7801 gtcgggttcg agcagatcgt tgatgccgac cacctcgatg tcgtcaaagt gctgtaccgc
     7861 tgcgcgaaac accatccgtc cgatacggcc aaagccgttg attcctacct tgattgtcat
     7921 gaaacggtct ccttgagaaa ttacaacagt gatttcacgg tgttaaccac gttgttcacc
     7981 gtgaaaccga taaagtcaaa caacgggccg gccggagccg actctccaaa acgatcgata
     8041 ccgatcacgg tacgagtcca acatacttgc accagaagtt gccgacgccc gcttcgatgg
     8101 ccagacgcgg cacggcgtgg ggcaggacat ctcccgacag gaggcgtcct gcccggtggt
     8161 ggtttcgact ccggggggat gatcgacttc cgggtgcacc ggagtcttcg agacaaactg
     8221 atgaaaattc tgcggttcat cgatcgacac gtcgttgctg ccgaggtgca gcagcgtgaa
     8281 gaccggtaac aatccgtgat tgtgtgaggg cacggatcgg tcgagttcag gccagtctgg
     8341 attggccggg ttgtggcgca agtggcgacg ccagtggact tctgcgatat cgggcatgcc
     8401 catcggcacg ccagggtgaa cgaagttgac ttgctggacc gcgtccatgg ccagggcgcg
     8461 gatggcgcgg gtcagggggg caaaagcagt gggcacgatg actcgccgat ccaggaaatg
     8521 ggttttagtc gcatgtgcaa attatccgcc aaaacgggag gctttgccaa tttccgtagc
     8581 gacgccggtt tcggaattgc tgccgggcat tgatcatcgg cttgatcggt tcgttgtcaa
     8641 atgatttatt gaaagtaaaa gctaaacata aaacacaaaa agtttaactt ttaataatct
     8701 atcgcgcgtc ctacactgct tcgcaaccaa cgacgaacgc ctctcttcac ccttatcgac
     8761 acggagacca acatggacca atcgaatcgt tacgccgacc tcagcctcag ggaagaggac
     8821 ttgatcaagg gtggcaggca cattctcgtg gcttacaaga tgaagccgaa agcgggctac
     8881 gattatctgc aggcggcagc ccacttcgcc gccgagtcat ctaccggtac caatgtcgaa
     8941 gtgtgcacca ccgacgagtt tacccgcggc gtcgatgccc tggtctacct gatcgatgaa
     9001 gccggcgaag agatgcgcat cgcctatccg ctggacctgt tcgaccgcaa cgtcaccgac
     9061 gggcggatga tgatcgtatc gttcctgacg ctggtgatcg gcaacaacca gggcatgggt
     9121 gacatcgaac acgccaagat ctacgatttc tacatgcccg agcgtgccat tcagcttttt
     9181 gacgggccgg ccaaggacat ctccgacctg tggcggatcc tgggtcgccc ggtcaaggat
     9241 ggcggctata ttgccggcac gatcatcaag ccgaaactcg gtctgcgtcc ggagccgttt
     9301 gccaaggcgg cttaccagtt ctggctgggc ggggacttca tcaagaatga cgaaccgcaa
     9361 ggcaaccagg tttttgcacc aatgaagaag gtgatgccgc tggtgtacga tgccatgaag
     9421 cgcgcgcagg acgagaccgg tcaggccaag ctgttctcgg ccaacatcac tgccgacgac
     9481 cactatgaaa tgtgcgcgcg cgccgattac atcctggaga ctttcgggcc tgatgccgac
     9541 aaggtcgcct ttctcgtcga tggctacgtc ggcggtccgg gcatggtcac cactgctcgc
     9601 cggcagtatc ccaaccagta cctgcactac caccgtgccg gtcacggcgc cgtcacttcg
     9661 ccgagttcca agcgtggcta cgatgcctat gtgctggcca agatgtcgcg tctgcagggt
     9721 gcctcgggaa ttcacgtcgg caccatgggc tacggcaaga tggagggcag tgccgacgat
     9781 cgggcgatcg cctacgtcat cgagcgtgac gagtatcagg ggccggctta tcaccagaag
     9841 tggtatggca tgaagccgac cacgccaatc atttccggcg gcatgaacgc cttgcggctg
     9901 ccgggtttct tcgccaatct gggacacggc aacgtcatca acacttccgg tggcggttcc
     9961 tatggccaca tcgacagccc ggccgctggt gccatttcgc tacgacaggc ttacgagtgt
    10021 tggaaagccg gggccgaccc gatcgaatgg gccaggcagc acccggaatt cgcgcgtgct
    10081 tttgagtcct tccccgggga tgccgacaaa ctctatccgg gctggcgcga aaagctcggt
    10141 gttcatcgct agaggcaagg ggctggggta agggtgacgt gttgccggag cgtcgccccg
    10201 actacgggtt gagcgccgac gacatttcgg gagatgggca atgaccgaca aggggcaatt
    10261 cagggttctc gacgaaccgt actatcagcc acaaggcaat gaagtggcgc tctacgaagc
    10321 ggcctatgcg gcgcgtctgc cggtgatggt caagggaccg acaggctgcg gcaagtcgcg
    10381 cttcatcgaa tacatggcct ggcggctggg caagcccttg atcaccatcg cctgcaacga
    10441 ggacatgacc gcctccgatc tggtcgggcg ctacctgctc gaagccaacg gcacgcgctg
    10501 gctcgacggt ccgctgacca ccgctgcacg tatcggggcc atctgttacc tcgatgaaat
    10561 tgtcgaggcg cgtcaggaca ccacggtcgt cattcatccg ctgaccgacc atcggcgtac
    10621 gctgccgctc gacaagaagg gagaactgct gcgcgcccac cccgattttc agttggccat
    10681 ttcgtacaac cccggctacc aaagcctgat gaaggacttg aagcagtcga ccaagcagcg
    10741 cttcaccgcc ttcgagttcg aatatcctgc ggcggcactg gagatcgcca ttcttgccgg
    10801 cgaaagcggt atcgataccg tcaccgccgc acaactggtg aatgtcggcg cggccgcccg
    10861 ccggctcaag gggcacgggc ttgacgaggg catctcgacg cgtttgctgg tctatgccgc
    10921 gaccctgatc cgggcaggag tttcgccgcg tgaggcttgt cgcatggccc tggtgcggcc
    10981 aatcaccgac gaccgtgaca ttcgcgacac cctggatcac gccatcgacg cgaccttcgc
    11041 ctaggccagg gcgaagctcc cagccatgtc cgcgaacgtc actgacagca gggatcccgc
    11101 aacctgggat gagcgcctgc aaggctaccg tgcgcaactc gactgtcgct ttccgcaagt
    11161 cgccgaggtg ttcgagcgat gcctgatcga ggcgcttgcc gtgctctcga cagaaggggt
    11221 cgatgcctac ctcgaccatg cgcgcgtgct cagccgcatg gggcgcggtg tcgagccggt
    11281 gctggtgttt ctcgaggagt ggccgtcgat cgccgcaatc ctcggtgaac aggccttgcc
    11341 gtcgatcgcc cggaccattg acgcgcttgg caaatcgccg aatgcaaaag cgattttccc
    11401 ctttctgcag acgcttgccg cagtcgcgcg gcgtttgccg gcagcggcac agatgcagca
    11461 ctatctggat ctggcgcttg atctcatgca acgtaccagc gggtcgatcc acggcattca
    11521 ccagaccttc gccagcccgg cgctgcccga gttcttcggc caggcgccac ggctgctgca
    11581 tgcgctgagt atcgccggac tgagcaagtg ggttgaatac ggcattcgca attaccacaa
    11641 ccatccggaa cgacagcgcg cctacttcag cctgcagtcg ccggacagtc gcgcggttct
    11701 gcagcgtgag cgccacggca cgctgctggt cgaccatgag cgtcagctcg atctttacct
    11761 gcgaggtcta tggggcgata gcgccctcct ggtgccctat gcgacaaacc tggagcaatc
    11821 gccgcaatct gttccttact acgacgcttc tggaatacgc ctgccggatg tcttcgacga
    11881 tgcgcatggc gtctccggca ttgaccgcta ccgcgcgctg ctggcgcaca tcgcgggcca
    11941 tcggcgctgg agccgcccga tatttgccga caacttcagc ccgatgcagc gtctggcgat
    12001 cgaggtcttc gaggacagtc gcatcgaaga gctgatcctg cgtgcatatc ccggtctgcg
    12061 ccacatcttt ctcgccctgc acccgatacc gctggaaggc gcttgcgacc cggaaaccag
    12121 ttcctgtctt cgccaccggc tgaccatgct gtcgcgcgct cttctcgacc ccgaacatgg
    12181 ctatcaggac gtgccgttgc tggagttcgt ggatcgcttc cgggaaacga tggcccgcgg
    12241 cgaatccggc agcgaagaaa tggctgctct cgcgctggcc tacgtcgcgc gcacccgtcg
    12301 acaga
//
LOCUS       DAOSIT010000009        10962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_5893_length_10962_cov_12.897405, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000009 DAOSIT010000000
VERSION     DAOSIT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5893_length_10962_cov_12.897405"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLL14_00575"
     CDS             complement(<1..878)
                     /locus_tag="PLL14_00575"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG02437.1"
                     /translation="MKITVLGAGAWGTALAIAFSARHTVTLWARRAEVRSVLSADREN
                     RRLLPAHPFPETLRIGDDFAAAAAAADLLIIATPISGLRQTLCRLREAGVPRPLIWVC
                     KGLEAETAKLPHQVVAEELGPQALCGALSGPSFAEEVARGLPAAVTLAANDADFGHRT
                     ALALHSPHFRIYANDDLPGVEVGGGVKNIMAIATGVCDGLGLGLNARAALITRGLAEI
                     ARFGVALGGHPQTFMGLAGMGDLILTCTGDLSRNRRVGLALGAGKTLPQILRDLGHVA
                     EGVSTTREVARLAMQLG"
     gene            complement(885..1319)
                     /locus_tag="PLL14_00580"
     CDS             complement(885..1319)
                     /locus_tag="PLL14_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOG02438.1"
                     /translation="MKRLLSLLLTAGLLAPALAIEYRTVDTPAVLYDAPSQKGSKLFV
                     VRRGTPVEMVVNLEGWSKVRDADGGLAWIEARYLAKRRSVIVSVVRAQVRQSADEAAA
                     VVFEAEKNVSLDYLETMPGGWVRVRHRDGQTGFVRVNQIWGV"
     gene            complement(1332..1799)
                     /gene="secB"
                     /locus_tag="PLL14_00585"
     CDS             complement(1332..1799)
                     /gene="secB"
                     /locus_tag="PLL14_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOG02439.1"
                     /translation="MSEQETPIFAIEKIYIKDLSVEVPNAPQIFLERESPKVSIQLQT
                     NAQGIGEGVFEVVLTTTVTSKLEEKTVFLVEVGQAGIFRVQNVPSENMEPLLSIACPN
                     ILFPYAREVVSDAVTRAGFAPVLLQPVNFEALYMSRLQQQEAAKEASGESTIQ"
     gene            complement(1852..2127)
                     /gene="grxC"
                     /locus_tag="PLL14_00590"
     CDS             complement(1852..2127)
                     /gene="grxC"
                     /locus_tag="PLL14_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HOG02440.1"
                     /translation="MSIASRVLMYSTAVCPFCVRAEQLLRARGVVDIEKVRVDLDPAR
                     RAEMLERTARRTVPQIYIGETHVGGCDELIALDRAGKLLPLLAGACV"
     gene            complement(2124..2543)
                     /locus_tag="PLL14_00595"
     CDS             complement(2124..2543)
                     /locus_tag="PLL14_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG02441.1"
                     /translation="MEFIQQNILLVAVAVVSGSMLLALTFRRPGGTNAVTPTQATMLI
                     NRENAQLIDIREPNEYVAGHLGDSRNIPAGSLAERAAELEPFKDKPLILICQSGMRSS
                     AARSTLAKLGFLKVHTLDGGVAAWTEAGLPLKKGARR"
     gene            complement(2631..2939)
                     /locus_tag="PLL14_00600"
     CDS             complement(2631..2939)
                     /locus_tag="PLL14_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG02442.1"
                     /translation="MPITSSLIDKQEHIEQAARALKSISHPLRLKILCVIGDQEACVQ
                     EIVQAVGTSQSNISQHLAILREKGVLLARKESNRVYYRVGDARTLQLIGMMREVFCGG
                     "
     gene            3239..4594
                     /locus_tag="PLL14_00605"
     CDS             3239..4594
                     /locus_tag="PLL14_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG02443.1"
                     /translation="MPTHAITASHPAGARNADTASPNEVAERQGDLKSLRLQIDTLRR
                     EMAAAEGSRRDAVDQLKDAERAISTTQRDLLQLTTQIGSLQATLRDLGTQARALEQQL
                     SAQQAQLGKLLYRQYLRGTPDPLRLFLNGDDPNQLARDLHYLGLIGRARSQILQEIEA
                     TLHRKQALATDTRQQAEQLSASEAREKEAHAKLLAQREQRQAMLDKVSDHIATQRREI
                     GNLQRDERRMAELVERLSKIIATRAREAQEAHREAQRREAARQAAQQAAQQAARQAAR
                     QTTTQEKPARAARSLAEKPLVEKAPVATTQVPEAHVEPTPPASGNLARMKGQLRLPTR
                     GSISNRFGTPRQEGSTWKGLFILASTGSEVRSIAGGRVVFAEWMRGFGNLLIIDHGGS
                     YLSIYANNDSLLKQVGDEVRAAETIATVGNSGGNPESGLYFELRHQGKPLDPLAWVNP
                     K"
     gene            4617..6026
                     /locus_tag="PLL14_00610"
     CDS             4617..6026
                     /locus_tag="PLL14_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG02444.1"
                     /translation="MGKLKQAGLVCAGLVGGVLISLQFSAIADKETRASLPVEELRTF
                     AEVFNAIKTGYVEPVEDKKLITHAISGMLSNLDPHSSYLDADAFKDLQVGTQGEFGGI
                     GIEVGMEDGLVKVVSPIEDTPAYRAGVKPGDLIFKIDDTLVKGLTLNEAVKRMRGKPR
                     SQIRLSILRKGEAKPLEITLTREVIKVQSVKSKLVEDGYGYLRITSFQENTGAAVVKH
                     LQDLFKPGPMKGLVLDLRNDPGGLLNSAVGVSAAFLPLDTLVTSTDGRTPDAKHRFSA
                     SPADYLRGSRDDYIKALPPEARKVPMVVLVNGGSASASEIVAGALQDHRRAIVLGTTT
                     FGKGSVQTVLPLPGNTAIKLTTARYFTPSGRSIQAKGIVPDIVVEESANGSSAERLRE
                     ANLEHHLDGSKDPASAKDAPAKPAKTAPPAKPKASAPKDDPDEAAEPPSRELASKKDY
                     QLNQALNLLKGMQIMQNNR"
     gene            6050..6469
                     /locus_tag="PLL14_00615"
     CDS             6050..6469
                     /locus_tag="PLL14_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02445.1"
                     /translation="MKRPAAIPQTPIRKQRNILFAKFPPGQVPEASDFLGKLEDLELE
                     RREQPRALGVAYDLHHHCLQELEGTLEDQGYHLDNTLMSKMMRALIYYVEETQLHNLD
                     APVRPLKRSQSEAYTHAWERHPHGDHDDTPPEWREYK"
     gene            6483..7235
                     /gene="moeB"
                     /locus_tag="PLL14_00620"
     CDS             6483..7235
                     /gene="moeB"
                     /locus_tag="PLL14_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOG02446.1"
                     /translation="MNDGQLLRYSRHILLDEIGVEGQQRLLDAQALIIGAGGLGSPAA
                     LYLASAGVGHITLADGDTVDLTNLQRQILHTDDRIGESKACSGKIALQTLNPEVRVTA
                     VSRRLAGEALEALVDEADVVLDCCDNFATRHAVNRACVKLRRPLVSGAAIRFAGQLAV
                     FDARREDTPCYYCLFPEGEDVEELRCAVMGVFAPLTGIIGAMQAAEALKLIIGAGEPV
                     VGRLWLLDGLAMAWRCVKFARDPQCPVCAQRA"
     gene            complement(7232..8302)
                     /gene="ribD"
                     /locus_tag="PLL14_00625"
     CDS             complement(7232..8302)
                     /gene="ribD"
                     /locus_tag="PLL14_00625"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOG02447.1"
                     /translation="MARALRLAERGLFSTSPNPRVGCVIVRDGEVVGEGWHVRAGGQH
                     AEVHALQAAGDRARGATLYVTLEPCSHHGRTPPCAEALLVAGVARVVAAMQDPNPQVA
                     GRGLAHLRVAGVLAECGLLAAEAQELNVGFVSRMTRARPWLRMKLAASLDGKTALQNG
                     ASQWLTGAAARQDGHRWRARACAILSGIGTVRHDNPQLNVRAVEAPGAELRQPLKVIV
                     DSRLELPLDARLLRDGGVLIAAAADDCGRSAALVERGAEVIVLPGPGGQVDLVALLHE
                     LARRGINEVHAEAGFALNGSLLRAGVVDELLLYLAPCLIGDAARGMFDLPPLQSLADK
                     RTLQIRDLRMLGADLRVLARFG"
     gene            complement(8338..8808)
                     /gene="nrdR"
                     /locus_tag="PLL14_00630"
     CDS             complement(8338..8808)
                     /gene="nrdR"
                     /locus_tag="PLL14_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOG02448.1"
                     /translation="MKCPFCGADETAVADTRINDDGDIVRRRRRCLKCDKRFTTYERA
                     ELRLPQIIKKNGSRVDFDRDKLAASLSLALRKRPVSSAMVEAAIARIEEKLLALGERE
                     VASEKVGEMVMRELKKLDKVAYVRFASVYRNFEDVDEFSKVVREVSRSTPPSGR"
     gene            complement(8805..10064)
                     /gene="glyA"
                     /locus_tag="PLL14_00635"
     CDS             complement(8805..10064)
                     /gene="glyA"
                     /locus_tag="PLL14_00635"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG02449.1"
                     /translation="MFSDRDTLARVDPKIWQVLENENRRQEEHIELIASENYVSHAVM
                     AAQGSQLTNKYAEGYPGKRYYGGCEYVDVAEQLAIDRLKKLFGADAANVQPNSGSQAN
                     QAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFKVVAYGLDEHEAIDYAKMEA
                     LAREHKPRILIAGASAYSLRIDFERFANVAREIGAIFMVDMAHYAGLIAAGCYPNPVP
                     HADVVTSTTHKTLRGPRGGVILMKAEHEKAINSAIFPGLQGGPLMHVIAAKAVAFKEA
                     LSPAFRDYQEQVVANARVLSRVLSAERGLRIVSGRTESHVFLVDLRAKNITGKDAEAA
                     LGAAHITVNKNAIPNDPQKPFVTSGIRIGSPAMTTRGFTEFESETVAHLIADVLDAPG
                     DADVLKRVRADVGVLCRKFPVYGSSSG"
     gene            complement(10186..>10962)
                     /locus_tag="PLL14_00640"
     CDS             complement(10186..>10962)
                     /locus_tag="PLL14_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG02450.1"
                     /translation="VLGLVYFMNSEGGSIAALLAKMAEIIETSRATLPGWLGDLLPRD
                     SEALREHASHWLREHSTELQMLGKETGHVVAHVLIGMIIGGMVSLREANHNEHMGPLA
                     RALAERVFRLGEAFRRVVFAQVRISALNTLFTTLYLGVVLPVFGVHLPLTKTMVVVTF
                     IAGLLPVVGNLISNTVIFVVSLAHSPGVALSSLAFLVGIHKLEYFLNARIVGAQIRAK
                     AWELLTAMLLMESTFGLAGLVAAPICYAWLKDELSRRRLI"
BASE COUNT         2149 a         3566 c         3430 g         1817 t
ORIGIN      
        1 cccagttgca tcgccaggcg ggcgacctcg cgcgtcgtgc tgacgccctc ggcgacatgt
       61 ccgaggtcgc gcaggatttg cggcaaggtc ttgccggcac ccagagccag accgacgcgg
      121 cgattgcgcg acaggtcgcc ggtacaggtg aggatcagat cgcccatgcc ggcgagcccc
      181 atgaaggttt gcgggtggcc gccgagggcg acaccaaaac gtgcgatctc cgcgaggccg
      241 cgggtaatca aggccgcacg ggcgttgagc ccgagtccga ggccatcgca gacgccggtg
      301 gcgatggcca tgatgttctt cacgccgccg ccgacttcaa cgcctggcag gtcgtcgttg
      361 gcgtagatgc gaaaatgcgg gctatgcagg gccagcgccg tgcgatgccc gaaatcggcg
      421 tcattggctg ccagtgtcac cgccgccggc aagccgcgcg ccacttcttc ggcgaagctc
      481 ggtcccgaca gggcgccaca cagggcttgc ggacccagtt cttcggcgac gacctgatgc
      541 ggcagcttgg ccgtttcggc ctccaggccc ttgcacaccc agatcagcgg tcgcggcacc
      601 ccggcctcgc gcaggcgaca gagcgtctgg cgcagaccag agatcggcgt ggcgatgatc
      661 agcagatcgg cagcggcggc ggcggcggca aaatcgtcgc caatccgcag ggtctccggg
      721 aaagggtgag cgggcagcag gcggcggttt tcgcgatcgg cggacagcac gcttctgact
      781 tcggcccggc gcgcccacag ggtaacggtg tggcgggcgc tgaaggcgat ggccagggcc
      841 gtaccccagg cacccgctcc caacacggtg atcttcatgg ccgctcagac cccccagatc
      901 tggttgacgc gcacgaagcc tgtctggccg tcgcgatgcc gcaccctgac ccagccaccg
      961 ggcatggttt cgagatagtc cagtgaaacg ttcttttccg cttcgaagac gaccgccgcc
     1021 gcctcgtcgg cgctctgccg aacttgcgcg cgcaccacgc tgacgatgac cgaacgccgc
     1081 ttggcgagat acctggcctc aatccaggcc agaccgccgt cggcatcacg gaccttggac
     1141 caaccttcga gattgacgac catctcgacc ggcgtgccgc gcctgacgac gaagagcttg
     1201 ctgcctttct gcgaaggcgc atcgtaaagc accgctggtg tatcgacggt gcggtactcg
     1261 atcgccaacg ctggcgccaa caatccggcg gtcagcagca ggctcaacag ccgcttcatc
     1321 gtgcggcccg cttactggat cgtcgactcg ccggaggcct ccttggcggc ttcctgctgc
     1381 tgcagacgcg acatgtagag cgcttcgaag ttgaccggct gcagcagcac gggtgcgaaa
     1441 ccggcacgcg tcacggcatc ggagacgact tcacgcgcat acgggaagag gatgttcggg
     1501 caggcgatcg acagcagcgg ttccatgttt tcgctcggca cgttctgcac gcggaagatg
     1561 ccggcctgac cgacctcgac cagaaacacc gtcttctctt cgagcttgga ggtcacggtg
     1621 gtggtcagga cgacctcgaa gacgccctcg ccaatcccct gcgcgttggt ctgcaactgg
     1681 atgctgacct tgggagactc gcgttccaga aagatctgcg gcgcgttggg cacttcaacc
     1741 gaaagatcct tgatgtagat tttctcgatc gcaaagatgg gggtttcttg ttcgctcatg
     1801 gtagtttctt ggccaatggg atgagggggt tagtgaggct cggggcgggc atcaaacgca
     1861 tgcacccgcg agtaagggca gcaacttgcc cgcacgatcg agagcaatca actcgtcgca
     1921 accaccgacg tgcgtttcgc cgatgtaaat ctgtggaacc gtacgcctgg cggtcctttc
     1981 gagcatctcg gcgcggcgcg cgggatcgag atcgacccgc actttctcga tatcgacaac
     2041 accgcgcgcg cgcagcaact gctcggcacg tacgcagaaa ggacaaaccg cggtcgagta
     2101 catcagcacg cgtgacgcga tgctcatcgc cgggctccct tcttcagcgg cagaccggct
     2161 tccgtccatg ccgcaacacc accatcgagg gtgtgcacct tgaggaaacc aagcttggcg
     2221 agagtcgaac gggcggcgga cgagcgcatt ccggactggc agatgaggat cagcggcttg
     2281 tccttgaatg gctccagctc ggccgcgcgt tccgcgagcg atcccgcagg aatgttgcgc
     2341 gagtcgccaa ggtgcccggc aacgtattcg ttcggctcac gaatgtcgat caactgggcg
     2401 ttctcgcggt tgatcagcat cgtcgcctgc gtcggcgtga cggcgtttgt cccccccggc
     2461 cggcggaaag tgagcgccag cagcatcgag ccactgacca cggcgacggc caccagaaga
     2521 atgttctgct gtatgaattc caatcgagac tccagaaact gacccggctc aacagacggg
     2581 agcgggatgc cgctccccgg caaaaaacat cggcgataac aaaaaaaccc ctagccgccg
     2641 cagaaaacct cgcgcatcat gccaatcaac tgcagcgtgc gggcatcgcc aacccgataa
     2701 taaacgcggt tggactcctt gcgcgcgagc agaacaccct tttcgcgaag aatggcaaga
     2761 tgctgggaaa tgttgctttg cgaagtgcct acggcctgga cgatctcttg aacacaggct
     2821 tcctgatccc cgatcacgca aaggatcttg agtcgcaggg gatgtgatat cgacttcagc
     2881 gcacgcgctg cctgctcaat atgctcctgc ttgtcaatga gactgctcgt gatcggcacg
     2941 atacgccaga ttcgtcgttt atcatataat cgccgattat agaacaaaac cttgatgcaa
     3001 gcgtgtcccg cacgccatcg ccctgacggt agcctcccgc gcgctggctc gatcgaggtt
     3061 tctgcaaggc gtgtgccgtt cattgacgaa taccccagac aaatccccgg cccatgccgc
     3121 gccgggcgct accagcgccc cggtagcggg gccggcgatc accatgccac cccgcagatc
     3181 agcgaggctc ccaccgctgc tccggtgctg gttgctgatc gggtggttgg cgttggcatt
     3241 gccgacacac gcgatcaccg ccagtcaccc ggcgggtgcc agaaacgccg acacggcttc
     3301 gccgaacgaa gtcgccgaac ggcagggcga cctcaagtcg ctacgtttgc agatcgatac
     3361 gctgcgcagg gagatggctg ccgccgaagg gtcgcgccgg gacgcggtgg atcagttgaa
     3421 ggatgccgag cgcgcgatct cgaccaccca gcgcgacctg ctgcaactga ctacccagat
     3481 cggtagcctg caggcaacgc tcagggacct cggcacgcag gcgcgcgcac tcgaacaaca
     3541 actgagcgca cagcaggcgc aactgggaaa gctgctctac cgacagtatc tgcgaggtac
     3601 tccggatcct ctgcgactgt tcctcaacgg cgacgatccc aatcaactgg cgcgggatct
     3661 gcactatctg gggctgattg gcagagcccg cagccagatc ctgcaggaaa tcgaggccac
     3721 cctgcaccgc aagcaggcgt tggctaccga cacccggcag caggccgagc aattgtcggc
     3781 cagcgaagcc cgcgagaagg aagcgcacgc caaattgctg gctcaacgcg agcagcgcca
     3841 ggcgatgctg gacaaggtct cggatcacat tgcgacgcag cgccgcgaga tcggcaatct
     3901 gcagcgtgac gagaggcgaa tggccgaact cgtcgaacgc ctgtcgaaaa tcatcgccac
     3961 cagggcccgt gaagcgcagg aagcccaccg cgaagcacaa cggcgcgagg cggcgcgaca
     4021 ggctgcgcag caggcggccc agcaggccgc gcggcaggcg gcgcgacaga cgacaacgca
     4081 agaaaaacct gcgcgggcgg cacgatcgct cgccgagaaa ccactcgtcg aaaaagcgcc
     4141 cgtcgcgacg acccaggttc cggaggcgca cgtcgagcct acgccgccgg catcgggcaa
     4201 tctggcaaga atgaaaggac aactgcgcct gccgacgcgc gggtcgatca gcaatcgctt
     4261 cggtacccca cgccaggagg gcagcacctg gaagggcctg ttcatcctcg ccagcaccgg
     4321 cagtgaagtc cgcagcatcg ccggtggccg ggtggtcttt gccgaatgga tgcgcggttt
     4381 tggcaatttg ctgatcattg accatggcgg cagctatctt tcgatctatg ccaacaatga
     4441 ctcgctgctg aagcaggtcg gcgacgaggt gcgcgccgcc gaaaccatcg ccaccgtcgg
     4501 caacagcggt ggcaatcccg aatccggttt atactttgag cttcgccacc aagggaagcc
     4561 gctggacccg ctggcttggg ttaacccgaa atgaggtgcg cactggagtg atacgaatgg
     4621 ggaagttgaa acaggccggg ctggtttgtg ccggattggt tggcggcgtt ctgatcagcc
     4681 tgcagttttc ggcgatcgcc gacaaggaaa cgcgagccag tctgcctgtt gaggaactgc
     4741 gcaccttcgc cgaggtattc aacgccatca agaccggcta cgtcgagccg gtcgaagaca
     4801 agaagctgat cacgcacgcg atcagcggca tgctctccaa tctcgacccg cactcaagct
     4861 atctcgatgc cgatgcgttc aaggacctgc aggtcggaac gcagggcgag ttcggcggca
     4921 tcggcatcga agtcgggatg gaagacgggc tggtcaaggt ggtttcgcca atcgaggaca
     4981 cgccggctta ccgcgccggg gtaaagccag gcgatctgat cttcaagatc gacgatacgc
     5041 tggtcaaggg tctgacgctc aacgaggccg tcaagcgaat gcgcggcaag ccgaggtcgc
     5101 agatcaggct gtcgattctc cgcaagggcg aggccaaacc cctggaaatc accctgacgc
     5161 gtgaagtcat caaggtgcag agcgtcaagt cgaaactcgt cgaggacggt tacggctatc
     5221 tgcgtatcac ctcgttccag gagaataccg gcgcggcggt ggtcaagcat ctccaggacc
     5281 tgttcaagcc tggcccgatg aagggcctgg tgctcgatct gcgcaacgat ccgggcgggc
     5341 tcctcaacag cgcggtcggc gtttcggcag ccttcctgcc gctggacacc ctggtcacct
     5401 cgaccgatgg gcgcaccccg gacgccaagc accgtttctc ggcatcgccc gcggactacc
     5461 tgcgcggtag tcgggacgac tacatcaagg cgctgccgcc cgaggcgcgc aaggtaccga
     5521 tggtggtgct ggtcaatgga ggctcggcct cggcatcgga aatcgtcgcc ggggcgctgc
     5581 aggaccaccg ccgtgcgatt gttctcggga cgacgacttt cggcaaaggc tccgtacaaa
     5641 ccgtgttgcc cttgcccgga aacaccgcga tcaaactgac caccgcacgc tacttcacac
     5701 cttcgggccg ctcgatccag gccaaaggca tcgtgccgga catcgtcgtc gaagagagcg
     5761 ccaacggcag ttcggccgag cgcctgcgcg aggccaacct cgaacaccat ctcgacggca
     5821 gcaaggaccc ggcgtcggcc aaggacgcgc cggcgaagcc tgccaagaca gcgccacccg
     5881 ccaagccgaa ggccagcgca cccaaggacg atccggacga ggccgccgag ccgccgagcc
     5941 gcgaactggc gtcaaagaag gactatcagt tgaaccaggc gctgaacctt ctcaagggga
     6001 tgcagatcat gcagaataat cggtgagcct ttctctcgga gcggggaacg tgaaacgacc
     6061 ggccgccatc ccacagacgc cgattcgcaa gcagcgcaac atcctgtttg ccaagttccc
     6121 gcccggccag gtccccgagg cgtcggattt tctcggcaag ctggaggacc tcgaactcga
     6181 acggcgcgaa cagccgcgcg cactcggtgt ggcctacgat ctgcaccacc attgtctgca
     6241 ggaactcgaa ggcacgctcg aggaccaggg ttatcacctc gacaacacgc tgatgagcaa
     6301 gatgatgcgc gcgctgatct actacgtcga ggaaacgcaa ctgcacaatc tcgacgcgcc
     6361 ggtgcggccg ctcaagcgct cacagtcgga agcctacacg cacgcctggg aacgtcaccc
     6421 gcacggcgac cacgacgata ctccgccgga gtggcgcgaa tacaagtaat gcgctcctgc
     6481 cgatgaatga cgggcagttg ctgcgctaca gccggcacat cctgctggac gagattggcg
     6541 tcgaaggaca gcagcgcctg ctcgatgcgc aagcgctgat catcggcgcc ggcggtctcg
     6601 gttcgccggc ggccctgtat ctcgcgtctg ccggcgttgg ccacatcacg ctggcggacg
     6661 gcgacacggt agacctgacc aacctgcagc ggcagatcct gcacactgac gaccgcatcg
     6721 gcgagtccaa ggcctgctcc ggcaagatcg cgttacagac cctcaatccc gaggtacggg
     6781 tcacagcggt cagccgtcgg cttgccggcg aagcgctgga agctttggtc gacgaggccg
     6841 atgtggtgct cgactgctgc gacaacttcg ccacccgcca cgccgtcaat cgggcctgtg
     6901 tgaagttgcg ccggccgctg gtttccggag cggcgatccg ctttgccgga cagcttgcgg
     6961 ttttcgatgc tcggcgcgaa gacacgccgt gttactactg tctctttccc gaaggggagg
     7021 acgtcgagga gttgcgttgc gcggtgatgg gcgttttcgc accgctcacc ggcatcatcg
     7081 gcgcgatgca ggcggccgag gcactgaaac tgattatcgg ggccggcgag ccggtcgtcg
     7141 gccggctctg gttgctcgac ggcctggcga tggcctggcg ctgcgtgaaa ttcgcgcggg
     7201 atccgcaatg cccggtctgc gcgcagcgcg cctagccgaa gcgggccagc acgcgcagat
     7261 cggcgccgag catgcgcagg tcgcggattt gcagcgtgcg tttgtcggcc agcgattgca
     7321 atggcggcag gtcgaacatg ccgcgcgccg cgtcgccgat caggcatggc gccaggtaaa
     7381 gcagcagttc gtcgaccaca cccgcccgca acagcgaacc attgagcgcg aaaccggcct
     7441 cggcgtgcac ttcgttgatg ccgcgccgcg ccagttcgtg aagcagcgcc accagatcga
     7501 cctgtccgcc cggtcccggc agcacgatga cttcggcgcc tcgctcgacg agggccgcgc
     7561 ttcggccgca gtcatcagcg gcggcagcga tcaggacacc gccatcgcgc aacaagcggg
     7621 cgtcgagcgg cagttcgagc cggctgtcca cgatcacctt caagggctgg cgcaactccg
     7681 cgcccggagc ttcgacagca cgcacattga gttgtgggtt gtcgtggcgg accgtcccga
     7741 tgccgctcag aatggcgcac gcgcgcgccc gccagcgatg cccgtcctgc cgggctgccg
     7801 cgccggtcag ccactggctg gcaccgttct gcagggccgt cttgccgtcg aggctggcgg
     7861 caagtttcat ccgcaaccac ggccgcgcac gcgtcatgcg cgagacaaaa ccgacattga
     7921 gttcctgcgc ctctgcggcc agcaagccac actcggccag caccccggcc acacgcaagt
     7981 gcgccagccc ccgtccggcc acttgcggat tgggatcctg catcgctgcg acgacccgcg
     8041 ccaccccggc gacaagcagg gcttcggcgc agggaggggt gcgaccatgg tgactgcagg
     8101 gttccagggt aacgtaaagg gtcgccccgc gcgctctgtc gcccgcggcc tgcagcgcgt
     8161 gaacttcggc gtgctgcccg ccggcacgta cgtgccagcc ctcgccgacg acctcgccat
     8221 ccctgacgat gacacagccg acgcgcggat tggggctggt cgagaagaga ccgcgctcgg
     8281 ccaggcgcag ggcgcgtgcc atgtgtgcat ggtcagaggc gctgaacgcc ggggccatca
     8341 gcggccggac ggcggcgtcg agcgagagac ttcgcgaacc accttggaga actcgtcgac
     8401 gtcctcgaaa ttgcggtaca ccgaggcaaa acgaacgtag gcgaccttgt cgagcttctt
     8461 caactcgcgc atgaccatct cgccgacttt ctccgaagcc acttcacgct cgccgagcgc
     8521 cagcagcttt tcttcgatac gggcaatcgc cgcctcgacc atcgcgctgc tgaccggtcg
     8581 tttgcgcagc gccagcgaaa ggctggcagc cagtttgtcg cggtcgaaat cgacccgcga
     8641 gccattcttc ttgatgatct gcggcaggcg cagctccgcc cgctcgtaag ttgtaaaccg
     8701 tttgtcacac ttcaggcaac gccgccgccg ccggacgatg tcgccatcgt cgttgatccg
     8761 ggtgtcggcg acggctgttt cgtcagcgcc gcagaacggg catttcatcc cgacgacgag
     8821 ccatagaccg gaaacttccg gcacagcacg ccgacatcgg cgcgcacgcg cttcaggaca
     8881 tcggcgtcgc ccggcgcatc gagtacatcg gcaatgaggt gcgccaccgt ctcggattcg
     8941 aactcggtga agccgcgcgt ggtcatcgcc ggcgagccga tgcggatgcc ggaagtcacg
     9001 aagggcttct gcggatcgtt cgggatcgcg ttcttgttaa ccgtgatgtg ggcggcaccc
     9061 agagccgcct cggcgtcctt gccggtgatg ttcttggcgc gcagatcgac cagaaacaca
     9121 tgcgattcgg tacggccgga gacgatccgc agaccgcgct cggccgacag cacgcgcgac
     9181 aatacgcggg cattggccac cacctgctcc tggtagtcgc ggaaagccgg tgacagggct
     9241 tccttgaaag ccaccgcctt ggcggcgatc acatgcatca gcggtccccc ttgcaggccg
     9301 gggaagatcg ccgaattgat cgctttttcg tgttcggcct tcatcaggat gacgccgcca
     9361 cgcggcccac gcagtgtctt gtgcgtggtc gaggtcacca catcggcgtg gggcaccggg
     9421 ttggggtaac aaccggccgc gatcaggccc gcgtaatgcg ccatatcgac catgaatatc
     9481 gcgccgattt ccctggccac attggcgaaa cgctcaaagt cgatgcgcag ggaataggcc
     9541 gaggccccgg caatcaggat gcgtggcttg tgctcgcgcg ccagcgcttc catcttcgcg
     9601 taatcgatcg cctcatgctc gtcgagacca taagccacaa ccttgaacca cttgccggac
     9661 atgttgagcg gcatgccatg cgtgagatgg ccaccctcgg cgaggctcat gcccatgatc
     9721 gtgtcgccgg gtttggcaaa ggccatcagc accgcctgat tcgcctgcga tccggaattc
     9781 ggctggacat tggcggcatc ggcgccaaac agtttcttca gtcggtcgat cgccagttgc
     9841 tcggcgacat cgacgtactc gcaaccaccg taatagcgct tgccggggta accctcggca
     9901 tacttgttgg tcagttgcga gccctgcgcc gccatcaccg cgtgactgac gtagttctcg
     9961 gaggcaatca gttcgatgtg ctcttcctga cggcgattct cgttctcgag cacctgccag
    10021 atcttggggt ccactctcgc cagggtgtct ctatcggaaa acatcatgcg cctcgcaagc
    10081 cataaataga agcggattgt agcacgcagc aggattccca cagaatctgc gccggatcca
    10141 caaccagccg gtggccaccg acgctcgcca tgaaagcgcg aacgctcaga tcaggcgccg
    10201 ccgtgacagt tcgtccttga gccaggcgta acaaatcggc gccgcgacca gaccggcaag
    10261 gccaaacgtg ctctccatga gcagcatggc ggtcagcaat tcccaggcct tggctctgat
    10321 ctgggcgccg acgatgcgtg cgttcaggaa atactccagc ttgtgaatcc cgaccagaaa
    10381 agccagcgag gaaagcgcca cgcccggcga atgcgccaga ctgaccacga agatcaccgt
    10441 attggaaatc agattgccaa ccaccggcag caggccggcg atgaaggtca cgacgaccat
    10501 cgtcttggtc agtggcaggt gtacgccgaa cactggcagc accacaccca ggtacaaggt
    10561 agtgaacagg gtattcaacg cggagatccg gacctgggcg aagaccacac gccggaaggc
    10621 ttcacccagg cgaaagacac gttctgccag cgcgcgcgcc aatggcccca tgtgctcgtt
    10681 gtgattggcc tcgcgcagcg acaccatacc gccgatgatc atgccgatca ggacgtgtgc
    10741 gacgacgtgg ccggtctcct tgccaagcat ctgcaactcg gtcgaatgtt cccgcagcca
    10801 gtggctggcg tgttcgcgca gggcctcgct gtccctcggg agcaggtcgc cgagccagcc
    10861 gggcaacgtc gcgcgcgagg tttcgatgat ctcggccatc tttgccagca acgcggcgat
    10921 gcttccaccc tcgctgttca tgaaatacac cagcccaagc ac
//
LOCUS       DAOSIT010000010        10806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_5987_length_10806_cov_9.073389, whole genome shotgun sequence.
ACCESSION   DAOSIT010000010 DAOSIT010000000
VERSION     DAOSIT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5987_length_10806_cov_9.073389"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1391)
                     /locus_tag="PLL14_00645"
     CDS             complement(<1..>1391)
                     /locus_tag="PLL14_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02451.1"
                     /translation="ALDAAGRLRLTPLTPGLIDLRVTATDRDGFTATRTHSVRVKDPS
                     DNLAPQLAWGGTLGSALAGSAAVEVHTPTALSANLREAQLMGWRLQVAAAGSDAWTTL
                     AEQESRAIALDQPIALGNIDPGRLSNGVWRLRLTAWDLAGRSSEIESRIVVDSTDKAL
                     PEALVTDASYALGGHTLALTRLLSSLSSPAAQDEPTGMNPGGDFGNWALPLLDTQLSS
                     DQPATLASGATAAWLEGARVWLNVPEDLSAVTTGVRSLSFTLTTGADRLGNEADAAQV
                     RHPQFSSSHGWTLEAYATAGSNTADSVLRQGNRLYDGVSGLPWVPAAYTLIAPTGDRY
                     ELDGQGHIKRIAFADGAQWLVSDAGVAALTADGRPAERLEFARDGQGRIVHISGVRQG
                     QTEPSSTAYRYDHQGRLVLVRALDTADDGTHYGYAPDGRLLTDMLSAHLGTPSRWDGV
                     GNTWTGELSGTAV"
     assembly_gap    1392..1491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1492..>5412)
                     /locus_tag="PLL14_00650"
     CDS             complement(<1492..>5412)
                     /locus_tag="PLL14_00650"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOG02452.1"
                     /translation="AADGAILQRLKAPGGDPLTHALAIDPQGGKIYVSSGRGIEVYNP
                     AESDPARAWRHFSTQRVGDLAFAPDGRLWAVKWSGSEIASAAPTPSSEILSFPMNGRD
                     VGRAELEYALPGVIDSIAFGAADTPLAGLLLASSQLGQRPVFTAGEATPHQSAVWMIE
                     LASRERLQLASGGTRGESIVATHDGRVLVAETGHIDEIAPWRAPLVKAVTVPDGALLP
                     LPLNTIGVVFDQDMWIGDADDGGSVLNVDNYTLSTASDSTTTASSRHPATIRWDAGTR
                     TAWLDVAGLQAGEYRLHIASRLQSAAEIPLRNDFETRFTALRDLSNRIRLDFSHTRAD
                     RATGEISYDVSVTNIGSEVIEGPLTLLLDPGQHFADIIAGATVGGGDQADLWVLDLGS
                     ALRALGGRLSAGATITDQTVSIVHGNRLAGALSLRAGMAELVKANLGHGIYALPQDTL
                     PPTLSVAGESDADELPSAQVGEDWSAQIEAIAADATRLYWQLLDALAGVSLTPSTATD
                     SDAGTLAIATLNWTPTARAAAASEIRVRVEDSRGGFAWRRFRIAVAGGNRAPTFEPIE
                     ELTLKEGETLTLPLRAADADGDHLTQIIRNLPPGATFDAARGLLTWTPGYDQAGVWSD
                     ITHIVSDGNATSSRNLAITVEQAYPKPVLDPLPVQALREGESYALQLAGFVPGRAQVD
                     GSTLRLEYGAPWLPGGATLNPETGWLAWTPSYNQHGRYTVPVTLTASWTATDGTVSET
                     RIGRELVLDVANANGAPIFAPAETWNVVEGQPLRVSVFAFDPDNPEFAPKVRLRPDAD
                     AVGPETTPATVSYESSGLPPGAVFDAETLEIVWTPAYDQSGSYAITVTATDNGDPSSG
                     AGAAAGTAAVSQLHLPIVVSNANRAPSVADIPNTFVERGSVIEIPVFAEDADARLAGV
                     PITLSFSGLPRFARFIENSALDSTHGPVAGPGEVRGVLRVAPAEGDRGDYTITLTATD
                     DGDSSVGTETAAGALSEARSFVLSVRSFSEAPQINAPRQVVAVVGQPLAVAVAASDLD
                     QDPLTWIVDGLPLGAELKLASQYGQATLAWTPTADDIGNHEIELIVSDSGLPPRDAGY
                     IEPADPVPNVSRHRLRIVVRAENAAPELVGLQVDGERIADNGGTLRVDAREGTPLAID
                     FFGDDADADFIDWQVLDLPPGMQATAADNRLSLSWTPDRFAAQDGNTATLAPGVWRFS
                     VSGSDGMATFSRTLEVAVANVNQGPRLLPLPLQLVNEGDTLHFTVRAADADHDAVQLT
                     LLHDENTPPGVAFNPASGSFEWTPDQDTVDNGNADSRPYTFTFRA"
     assembly_gap    5413..5512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5513..>10806)
                     /locus_tag="PLL14_00655"
     CDS             complement(<5513..>10806)
                     /locus_tag="PLL14_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOG02453.1"
                     /translation="IDPQPFADLVTSAVVAPDQAVHGASIEVRYQVTNHGSAATRGES
                     AALDTWTDSIWLARDKRRPGAYKGDILLASFTHIGKLGVGEDYLATTQVAIPDDALSG
                     QYYLTVWSDTYDVMLEDTLATHINPDDPSQIDNNNYLARPISILGSTPPDLVLSQVVA
                     PEMASAGDAYTFSYTVENHGERFTGSWVDAVYLTDNADWNAAREIWHLGNFAQSRSLG
                     NGEKYTLTQTLPLAPSVSGRHLIVRTDIANQVGETGESNNSRAAASLVTTRAADLRVT
                     ELRSEAIASSGEETTVTWTVGNFGAAVWSGTRGWVDAVYLSRDPEFITERAIPLGTFN
                     HANLGGLAAGGSYTTSARVRLPAGSEGRYYLYLITDAESDVGDPRLRQSRRELTSGDN
                     TNAREAYYARSAYEGARNDNNLGRALLDVAYREPDLQIDEIVVSDRDARSGTPITVTW
                     TVSNRGTRQTRSSTWFDGLYLSRDASLDDGDYPLLDRGSPSEVSLGIRSISLTENGKP
                     KYLQVGESYTASATFKLPDSISGDFHLIVRADTAFARNPSERSTIRDGLPGMGRFGDG
                     TGTLQEFRDEGNNEASATLSVALATPPDLQVSEVSIPTSVLAGQPFSVDWRVRNAGGD
                     TPDDQGAWNDLVYLSRDRFLDLDQDRYLGYLAHHGALTAAGSYDAHLQVSAPRDLEGP
                     YYLFVISDPARAWGSGETGKVREFGHEQNNAATAPQPILVEAPPPADLTATQVLLPAS
                     AGVGDEIRVDFTIGNDSINTAYGRWTDAIYLSADNTWDLGDRLIGMVEHVGDLPGHGS
                     YSGTLSTPLPPIRDGNWRVIVRPDLFNEVFEGRIAYTASGLALPPGEVNNRMASGATL
                     QLRVPELPVGSLVTSSLSPGEARVYKISVAAGETLRVSLDAAAAEGSNEVYLRYGDLP
                     TGTVFDAAYSNPVAADQEALVPSTRAGDYYLLVRARQGAANSPINLRADLLPLSITRI
                     TPDHGGTGDDAQRWITLDIHGSHFLPGALVKLSRPGIAEVEPDRWQVLDATHIRAVFD
                     SRGLPHGLYDLTIINPDGQRVTEAERYLVERGVETEVTLGIGGPRTLATGESATYSVS
                     LQNLGNLDTPYVRFDFGVPEMGVSADVLKGLQLPYLVFGSSVGGRPDGRTIDLAGNTR
                     SYGTTPSDGTARGDIPWARLDGADNDRGSNLAPGYVFDLQAGGFVGTSFTLQTYPGLA
                     EWLAYDFAGLRNRLYALRPDWQEQGLLDGGVADLDRIADGLSRKFLSRVPDEHLTRLE
                     ALALPFRFDVAGAATPLTRDEFIADQQAHAQKLRAAILADSAAPPSLAALAADAAQWQ
                     TGWLAALEAAGLLRPLDEAPPIRDHPQVLSLNATLATGILLGKGGDSYRTEADLLGFF
                     SKVQTWYGDTAGYAGDTHAAQAPVDYLEVRQIPEGSVEIPVPTLADAARFDFGASHTT
                     RFIDFNIFVGGQSELEYLRHIGVLDEDFKAVAGQALDLGRYLQQAAAETAAAATLLSV
                     RGPQVLRGDDGQGYVPADTPLPFNITFSNPSETPAGQLRLVSQLDPSLDARSFRLGDI
                     RIGDIQVHLPADQASFQGDFDFTASKGFILRISAGVEPSTGIATWLLQAIDPDSGEVL
                     RDATRGLLAGDLDGASNAAADASGSATDQLRRGFASYSVRAATSAAAGSEIAATARLL
                     VDAAPPIDSETARVRLDTRAPQTTLKVTTLTSAGAADAAPIFDLQWSSIDDLSGVKSV
                     TLYVAEDAGDFRIWQRQLDPQTTQALFTGEAGRHYEFLA"
BASE COUNT         1612 a         3244 c         3824 g         1926 t
ORIGIN      
        1 atgaccgcgg tgccggagag ttcgccggtc caggtgttgc cgacgccatc ccatcggctt
       61 ggcgtcccga gatgtgcgct gagcatgtcg gtcagcaatc ggccatccgg ggcgtagcca
      121 taatgcgtgc cgtcgtccgc ggtgtcgagg gcgcgaacga gcaccaggcg gccctggtgg
      181 tcgtagcgat aggcggtgct gctgggttcg gtctggccct gccgcacgcc actgatgtgg
      241 actatcctcc cctggccgtc gcgcgcgaat tcgaggcgct cggctgggcg accgtcggcg
      301 gtcagtgcgg cgacaccggc gtccgagacc agccactggg cgccatcggc gaacgcgatg
      361 cgcttgatat gtccttggcc atcgagttca tagcggtcgc cggtgggtgc gatcagtgtg
      421 taagcggcgg gtacccacgg caggccgctg accccgtcgt agagacgatt gccctggcga
      481 aggacgctgt cggcggtgtt gctgccggcg gtcgcgtagg cttcgagggt ccagccatgg
      541 ctgctgctga actggggatg cctgacctgc gccgcatccg cttcgttacc caggcgatcg
      601 gcgccggtgg tcagggtgaa ggataacgaa cgcacgccgg tcgtcaccgc cgacaggtct
      661 tccggcacat tgagccagac gcgggcgcct tcgagccatg cggcggtggc gccggatgcc
      721 agcgtcgccg gctggtcact gctcagttgc gtgtcgagca gtggtagcgc ccagttgccg
      781 aaatcgccgc cggggttcat ccctgtcggc tcgtcttggg ctgccggtga cgacagcgag
      841 gagaggaggc gcgtcagggc gagggtgtgc ccgccgagcg cgtagctggc gtcggtgaca
      901 agcgcctctg gcagagcctt gtcggtgcta tcgacgacga tgcggctttc gatctcgctg
      961 ctgcgaccgg cgagatccca ggccgtcagg cgcaggcgcc agacgccgtt ggacaggcgt
     1021 ccggggtcga tattgccaag ggcgatcggc tgatcgaggg cgatggcgcg gctctcctgc
     1081 tcggcgagtg tcgtccatgc gtcgctgccg gcagcggcga cctgcaggcg ccagcccatc
     1141 agttgcgctt cgcgaaggtt ggcgctcagc gctgtcggcg tgtgcacctc gaccgcagcg
     1201 ctgccggcga gcgcgctgcc cagcgtgcca ccccaggcca actgcggcgc caggttgtcg
     1261 gacgggtcct tgacgcgcac gctgtgcgtg cgggtggcgg tgaagccgtc acgatcggtg
     1321 gcggtcaccc gcaggtcgat cagaccggga gtgagtggcg tcaatcgcag gcgtccggcg
     1381 gcgtcgagcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgcggaag
     1501 gtgaaggtat aggggcggct gtcggcgttg ccgttgtcga cggtgtcctg atccggcgtc
     1561 cattcgaagc tgccgctggc gggattgaac gcgacgcccg gcggcgtgtt ctcgtcgtgg
     1621 agcagggtca actggacggc gtcgtgatcg gcatcggccg cgcgtaccgt gaaatgtagc
     1681 gtatcgcctt cgttgaccag ttgcagcggc agcggcagca ggcgcggccc ctggttgacg
     1741 ttggccaccg cgacctcgag cgtacggctg aaggtcgcca tgccgtcgct gccgctgacg
     1801 ctgaagcgcc acactccggg cgccagggtc gcggtattgc cgtcctgtgc cgcaaaacgg
     1861 tcgggtgtcc aggatagcga caggcggttg tccgccgcag tcgcctgcat gccgggcggc
     1921 aggtcaagta cctgccagtc gatgaagtcg gcgtcggcat catcgccgaa aaagtcgatc
     1981 gccagcggcg taccttctct ggcatcgacg cgcagcgtgc cgccgttgtc ggcgatgcgc
     2041 tcgccgtcga cctgcagccc gaccagttcg ggtgccgcat tctcggcacg cacgacaatg
     2101 cgcagcctgt gacggctgac gttggggacc gggtcggccg gttcgatgta accggcatcg
     2161 cgcggcggca agccgctgtc gctgacgatc agctcgatct catggttacc gatgtcgtcg
     2221 gcggttgggg tccaggccag cgtggcctga ccgtactggc tggcgagctt cagttcggcg
     2281 ccgagcggca gaccgtcgac gatccaggtc agcggatcct gatcgaggtc gctggccgct
     2341 accgcgacag cgaggggttg cccgacgacg gccaccacct gccggggtgc attgatctgt
     2401 ggtgcttcgc tgaagctgcg cacgctcagc acgaaactgc gcgcctccga gagcgcgccg
     2461 gccgctgttt ccgtgcccac ggacgaatcg ccgtcgtcgg tggccgtgag ggtgatcgtg
     2521 tagtcgccac gatcaccctc agcgggcgcg acacgcagga ctcccctgac ttcgccggga
     2581 ccggcgaccg gtccgtgcgt cgaatccagt gcggagtttt cgatgaaccg ggcaaagcgc
     2641 ggcaggcctg agaagctgag cgtgatcggc acgccggcca atcgcgcgtc ggcgtcttct
     2701 gcgaagaccg gaatctcgat cacgcttccg cgctcaacga aggtgttcgg gatgtcggcg
     2761 acgctcggcg cacggttggc gttgctgacc acgatcggca aatgcagttg gctgaccgcc
     2821 gcggtgccgg cagccgctcc agcgcctgac gagggatcgc cgttgtcggt ggcggtgacg
     2881 gtaatggcgt agctgccgga ctgatcgtag gccggtgtcc agacgatctc cagcgtctcg
     2941 gcgtcgaaga cggcgcccgg cggcaggccg ctgctctcgt agctgaccgt ggccggcgtc
     3001 gtttctggtc cgacggcgtc ggcatccggt cgcaggcgga ccttgggtgc gaattcgggg
     3061 ttgtcgggat cgaaggcaaa aacgctgact ctgagcggtt ggccttcgac gacgttccag
     3121 gtttccgcag gtgcgaagat cggcgcgccg ttggcattgg ccacgtcgag caccagttcg
     3181 cgcccgatgc gggtttcgct gaccgtaccg tcggtggcgg tccagctggc cgtcagcgtc
     3241 accggtacgg tgtagcgtcc atgttgattg taggatggcg tccacgctag ccagccggtc
     3301 tcggggttga gcgtcgcacc gcccggcagc cacggcgcgc cgtattcgag gcgcagcgtg
     3361 ctgccatcga cttgcgcccg gccggggacg aaaccggcga gttgcagggc gtaactctcg
     3421 ccttcgcgca gcgcctgtac cggcagcggg tcgagtaccg gctttgggta cgcctgttcg
     3481 acggtgatcg ccaggttccg gctcgaagtc gcgttgccat cgctgacgat gtgcgtgatg
     3541 tcgctccaga cgccggcctg atcgtagccc ggcgtccagg tcagcaggcc acgggcggcg
     3601 tcgaaggtgg ctccgggtgg caggttcctg atgatctggg tgaggtggtc gccgtcagca
     3661 tcggcggcgc gcaggggtag cgtgagtgtt tcgccttcct tgagtgtgag ttcctcgatc
     3721 ggctcgaagg tcggtgcgcg gttgccgccg gcgacggcga tccggaagcg acgccaggca
     3781 aagccaccgc ggctgtcttc gacgcgcacc cggatttcac tggccgccgc cgctctggcg
     3841 gtgggcgtcc agttcagggt ggcgatggcc agcgtgcccg cgtcgctgtc ggttgccgtc
     3901 gatggcgtca ggctgactcc ggccagcgcg tcgagcagtt gccagtaaag tcgtgtcgcg
     3961 tctgcggcga tggcttcgat ctgggcactc cagtcctcgc cgacctgggc agacgggagt
     4021 tcgtcggcat cgctttcgcc ggcgacactc agcgtcggcg gcagggtgtc ctgcggcaac
     4081 gcatatatcc cgtgcccgag gttggccttg accagctcgg ccatgccggc acgcaggctc
     4141 aaggcgcccg ccaggcggtt gccgtggacg atgctcaccg tctggtcggt gatcgtcgca
     4201 ccggcggaca aacgaccgcc cagcgcccgc agtgcgctgc ccagatcgag gacccagaga
     4261 tccgcctgat cgccgccgcc gacggtggcg ccggcaatga tgtccgcgaa gtgctgcccg
     4321 ggatcgagca gcagggtcag cggcccctcg atcacctcgc tgccgatatt ggtgacgctc
     4381 acgtcgtagc tgatttcgcc agtggcgcgg tcggcacgtg tatgcgaaaa atcgaggcgt
     4441 atgcggttgc tgaggtcgcg cagggcggtg aagcgggttt cgaagtcatt cctgaggggt
     4501 atttcggctg cgctttgcag gcggctggcg atgtgcaggc ggtattcgcc ggcctgcagg
     4561 ccggcaacgt ccagccacgc cgtacgcgtg ccggcatccc aacggatcgt ggccggatgc
     4621 cggctgctgg cggttgtcgt actgtcgctc gccgtgctga gcgtgtagtt gtcaacgttg
     4681 aggacgctgc cgccatcgtc ggcgtcaccg atccacatgt cctggtcgaa aaccacgccg
     4741 atggtgttca gcggcagggg cagcaatgcg ccgtcgggta ccgtgacggc cttgaccagc
     4801 ggagcccgcc acggcgcaat ttcgtcgatg tgcccggttt cggcgacgag gacccggcca
     4861 tcgtgcgtgg cgacgatgga ctcgccacgc gtaccgccgc tggcgagttg caggcgttct
     4921 cggctggcca gctcgatcat ccatacggcg ctctggtgtg gcgtagcctc gccggcagtg
     4981 aacaccgggc gctgcccgag ctggcttgag gcgagcaaca ggccggcaag cggggtgtcg
     5041 gcggcgccaa aagcgatgct gtcgatgaca ccggggagag catattcgag ttctgcacgg
     5101 ccgacgtcac gaccgttcat cgggaagctg aggatttcac tgctgggcgt gggcgcggcg
     5161 ctggcgattt cgctgccgct ccatttgacc gcccacagcc gtccatccgg cgcaaaggcg
     5221 aggtcgccga cgcgctgcgt gctgaagtgt ctccaggccc tggcaggatc agactcggcc
     5281 ggattgtaaa cttcgatgcc gcgtccggag gagacgtaga tcttgccgcc ctgcgggtcg
     5341 atggccagcg catgcgtcag tggatcgccg cccggcgcct tcaggcgctg caggatggca
     5401 ccatcggcgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccaggaa
     5521 ctcgtagtgc ctgcccgctt cgccggtaaa tagtgcctgt gtcgtctgcg ggtcaagctg
     5581 acgctgccag atgcggaagt cgccggcgtc ctcggcaacg tagagggtga ccgacttgac
     5641 gccagagagg tcgtcgatgc tgctccattg caggtcgaaa atcggtgccg cgtcagcagc
     5701 acccgccgag gtcaaggtcg tgaccttgag ggtggtctgc ggcgcacgcg tgtcgagtct
     5761 gacccgcgca gtttcgctgt cgatcggcgg tgcggcgtcg accagcagcc gagccgtggc
     5821 ggcgatttcg ctccctgcgg cggcgctggt ggcggcacgc acgctgtagc tcgcgaagcc
     5881 gcgccgcagt tgatcggtgg cagagcccga ggcatcggcc gcggcgttcg acgcgccgtc
     5941 gagatccccg gccagcagac cgcgagttgc gtcgcgcaga acctcgccgc tatccgggtc
     6001 gatggcctgc aacagccagg tcgcgatgcc ggtcgatggc tcgacgccgg cgctgatgcg
     6061 caggatgaag cccttgctgg cggtgaagtc gaaatcgccc tggaaactgg cctgatcggc
     6121 tggcaggtgg acctgaatgt cgccgatccg gatgtcgccg agccggaacg agcgcgcgtc
     6181 aaggctgggg tcgagctggc tgaccaggcg caactggccg gccggggttt cgctcgggtt
     6241 gctgaaggta atgttgaagg gtagtggggt atcggccgga acgtagccct ggccatcgtc
     6301 gccgcgtagt acctgcgggc cgcgcacgct gagcagcgtt gccgcggcag ccgtctctgc
     6361 ggcggcctgc tgcaggtagc gtccgagatc gagcgcctgg ccggcgacgg ccttgaagtc
     6421 ttcgtcgagg accccgatgt ggcgcaggta ttcaagctct gactggccac cgacaaagat
     6481 gttgaagtcg atgaagcgcg ttgtgtggct cgcgccaaaa tcgaaacgcg ccgcgtcggc
     6541 cagcgttgga accgggatct cgacgctgcc ctccgggatc tgccgcactt ccagatagtc
     6601 gaccggcgcc tgggcagcat gggtgtcgcc ggcgtagccg gcggtatccc cgtaccaggt
     6661 ctgcaccttg gagaagaaac cgagaagatc ggcttcggtg cgatagctgt cgccgccctt
     6721 gcccagcagg atgccggtgg ccagcgttgc attgaggctc agtacctggg ggtgatcgcg
     6781 gatcggcggt gcttcatcga gcggccgcaa caggccggcg gcttccagcg cggccagcca
     6841 gccggtttgc cattgcgctg cgtcggcggc cagtgcggca aggctgggcg gggcagcact
     6901 gtcggcaaga atcgctgccc gcaatttctg tgcgtgtgct tgctgatcgg cgatgaattc
     6961 gtcgcgggtc agcggcgtgg ctgcgccggc gacgtcgaag cggaacggta gggccagtgc
     7021 ttcgagacgg gtgaggtgtt catcgggaac cctggacagg aatttcctgg agaggccatc
     7081 ggcaatgcgg tcgagatccg ccacgccacc atcgagcagt ccttgctcct gccagtcggg
     7141 gcgcagcgcg tagagtcggt tgcgcagacc ggcgaagtcg taggccagcc attcggcaag
     7201 gcccggataa gtctggagcg tgaatgacgt gccgacgaag cctccggcct gcaggtcgaa
     7261 aacgtaaccc ggcgcgaggt tactgccgcg gtcgttgtct gcgccgtcca ggcgcgccca
     7321 aggaatgtcg ccgcgagcgg tgccgtcgct gggtgtggtg ccatagctgc gggtgttgcc
     7381 ggcgaggtcg atagtcctgc cgtctggccg gccgccgacg ctgctcccga agacgaggta
     7441 gggcagctgc agtcctttga ggacatcggc gctgacaccc atttcgggga cgccgaagtc
     7501 aaaacgcacg tagggcgtat cgagattgcc gaggttctgc agcgagacgc tataggtcgc
     7561 gctttcgccg gtcgccagcg tgcgtgggcc gccgatgccg agggtaactt cggtctcgac
     7621 gccacgctcg acgagatagc gctcggcctc ggtgacgcgc tgaccatccg ggttgatgat
     7681 cgtcaggtcg tagagcccgt gcggcagtcc gcggctgtcg aataccgccc ggatatgagt
     7741 ggcgtcgagc acctgccagc gatcgggttc tacctcggcg atgccgggtc gcgagagctt
     7801 gacgagcgcg ccgggcagga agtgcgaacc gtggatgtcg agcgtgatcc agcgctgcgc
     7861 gtcgtccccg gtacccccat ggtccggggt aatgcgggtg atcgacagtg gcaacagatc
     7921 ggcgcgcagg ttgatcggcg agttcgcggc tccctggcgg gcgcgtacca gcagatagta
     7981 gtcgccggca cgggtgcttg gcaccagcgc ctcctggtcg gcagccacgg ggttgctgta
     8041 ggcagcgtcg aagacggtgc cagtgggcag gtcgccgtag cgcaggtaaa cctcattgct
     8101 gccttctgcg gccgcggcgt cgaggcttac acgcagggtt tcgccggcgg cgacgctgat
     8161 tttgtagacg cgtgcctcac cgggactgag gctgctggtc accaggctac cgaccggcag
     8221 ctccggcacg cgcaactgca gcgtagcgcc ggaggccata cggttgttga cttcacccgg
     8281 cggcagcgcg aggccgctgg cggtgtaggc gatcctgcct tcgaagacct cgttgaagag
     8341 gtcgggccgg acgatgacgc gccagttgcc gtccctgatc ggcggcagtg gggtgctcag
     8401 tgtgccactg tagctgccgt ggccggggag gtcgccgacg tgttcgacca tgccgatgag
     8461 ccggtcgccg agatcccagg tattgtcggc agaaaggtag atcgcgtcgg tccagcggcc
     8521 gtaggcggta ttgatcgagt cgttaccgat ggtgaaatcg acgcgtatct catcgccgac
     8581 gccagcgctg gcaggcagca ggacctgggt ggcggtgagg tcggcgggtg gcggtgcttc
     8641 gaccagaatc ggttgcggcg cggtggcggc gttgttctgc tcatggccaa actcccggac
     8701 cttgccggtc tcgccgcttc cccaggcgcg tgccgggtcg ctgatcacga agaggtaata
     8761 ggggccttcg aggtccctgg gtgcgctgac ttgcaggtga gcatcgtagc tgccagcagc
     8821 ggtaagtgcc ccgtggtgtg caagatagcc gaggtagcgg tcctgatcga gatcgaggaa
     8881 gcggtccctg gagagataga cgagatcatt ccaggcaccc tgatcgtctg gcgtgtcgcc
     8941 gcccgcgttg cgcactcgcc aatcaacgct gaaaggctgt ccggcaagca cgctcgtcgg
     9001 aatgctgact tcgctcacct gcaagtctgg tggggtagcc agcgccacgg aaagcgtggc
     9061 cgacgcttcg ttgttgccct catcgcggaa ctcctgcagc gtgcctgtgc catcgccgaa
     9121 tcgacccatt cccggcaggc cgtcgcggat cgtgctgcgt tcgctggggt tcctggcgaa
     9181 cgcggtgtcg gcacgcacga tcaggtggaa gtcaccgctg atcgagtccg gcagcttgaa
     9241 ggttgccgag gcggtatagg actcgcccac ttgcaggtac ttcggcttgc cgttttcggt
     9301 gaggctgatg ctgcggatgc ccagcgacac ttccgacggc gacccgcggt cgagcagggg
     9361 gtagtcgccg tcatcgagcg aggcatcacg tgagaggtag aggccatcaa accaggtact
     9421 gctgcgcgtc tggcgtgttc cccggttgct gaccgtccag gtgacggtga tcggcgtacc
     9481 cgagcgggcg tcgcgatccg aaacgacgat ctcgtcgatc tgcaggtcgg gttcgcggta
     9541 ggcaacgtcg aggagggcac ggccaaggtt gttgtcgtta cgggcgcctt cgtaggctga
     9601 acgcgcgtag taggcctccc tggcgttggt gttgtcgccg gaagtgagtt cgcgacggga
     9661 ctggcgcagt cgtggatcac cgacgtcgct ttcggcatcg gtgatgaggt agaggtaata
     9721 ccggccttcg ctaccggccg gcagtcggac cctggcagaa gtggtgtagc tgccgccggc
     9781 ggcgaggcca ccaaggttgg cgtgattgaa ggtgccgagt ggaatcgcgc gctcggtgat
     9841 gaactcggga tcacggctga ggtataccgc gtcgacccaa ccacgggttc ccgaccagac
     9901 ggcagcaccg aagttgccga ccgtccacgt cacggttgtt tcctcgcccg aactggcgat
     9961 tgcttcactg cgcagctcgg tgacgcgcag gtcggccgcc cgggtggtga ccagtgaagc
    10021 ggcggcacgg ctgttgttgg actcgccggt ttcgccgacc tggttcgcga tgtcggtgcg
    10081 cacgatgagg tgacgtccgc tgaccgatgg cgccagcggc agcgtctgtg tcagagtgta
    10141 tttctcgccg ttgccgagac tgcggctctg tgcaaaattg ccgagatgcc agatctcgcg
    10201 tgcggcgttc cagtcggcgt tgtcggtgag gtagaccgcg tcgacccagc tgccggtaaa
    10261 gcgctcaccg tggttttcta cggtgtagct gaaggtatag gcgtcgccgg cactcgccat
    10321 ttcgggggcg acgacttggc tcagcaccag atccggtggt gtgctgccga ggatgctgat
    10381 cggccgcgcc aggtagttgt tattgtcgat ttggctcggg tcatcgggat tgatgtgcgt
    10441 agcgagcgtg tcttcgagca ttacatcata ggtatcgctc cagacggtca ggtaatactg
    10501 gccggagagc gcatcgtcgg gtatcgccac ctgcgtggtg gcgagataat cctcgccgac
    10561 gcccagtttg ccgatatggg tgaaggacgc gagcaggatg tcgcccttgt aggcgccggg
    10621 ccggcgcttg tcgcgcgcca gccagatgct gtcggtccag gtatcgagcg ctgccgattc
    10681 gccacgggta gcggcactac cgtggttcgt cacctggtag cggacctcga tgctggcacc
    10741 atgcaccgcc tggtcggggg cgacgaccgc gctggtgacc agatcggcga aaggctgcgg
    10801 atcgat
//
LOCUS       DAOSIT010000011        10685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6073_length_10685_cov_9.412041, whole genome shotgun sequence.
ACCESSION   DAOSIT010000011 DAOSIT010000000
VERSION     DAOSIT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6073_length_10685_cov_9.412041"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /gene="cobT"
                     /locus_tag="PLL14_00660"
     CDS             complement(<1..965)
                     /gene="cobT"
                     /locus_tag="PLL14_00660"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOG02454.1"
                     /translation="MHFSISPPATMPGLQARIDGKTKPPGSLGQLERLALQIGQVQQS
                     LSPSLDHPHLLVFAGDHGAARAGVSAYPQEVTWQMVENFLAGGAAINVFARHNGLQLQ
                     VIDAGVAHDFGARDGLVDAKIAFGTHNAIEQAAMSAAERDLALARGAAIARGLADQGC
                     RVMAFGEMGIGNTAAASLLTQQLTGAPLADCVGRGTGLDTVGLARKRTLLAQAVGRAA
                     LPPDADALLVLAEFGGFEMAMMAGAMLAAAERGILLLIDGFIVTSALLVATAIAPSLR
                     DYCIFCHRSAEPGHVVQLRALQAEPLLDLGLCLGEGTGAALAWPLV"
     gene            1031..1576
                     /gene="cobU"
                     /locus_tag="PLL14_00665"
     CDS             1031..1576
                     /gene="cobU"
                     /locus_tag="PLL14_00665"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOG02455.1"
                     /translation="MRELILGGARSGKSRLAEIRATESGLRVVYVATALPLDAEMSRR
                     IEHHRARRCPDWGLVVEPLDLAAALRRHAAADVCLLVDCLTLWLSNVLFSGEAACQAE
                     AGEAVCCQHLAGATQALIDLLPQLPGHVILVSNEVGCGIVPMAALSRLFADEQGRLNQ
                     RVAAVCDRVTLVAAGLPLTLK"
     gene            1660..2124
                     /locus_tag="PLL14_00670"
     CDS             1660..2124
                     /locus_tag="PLL14_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG02456.1"
                     /translation="MYKRIMVAVDESFMTDQVLHAAIELAQVTGAKLAICHAIDETIL
                     AQREVAMMLPNSVGKTEARLRLGAQAFLDRLAATARGLGVDAEVKIVESEQKHVSDML
                     IEAAAEWQADLLVVGTHGRRGIERYFVGSAAERLVRKAQLSLLLVRGEEPDE"
     gene            2764..3249
                     /locus_tag="PLL14_00675"
     CDS             2764..3249
                     /locus_tag="PLL14_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02457.1"
                     /translation="MYIVDDPTLALIARFVGGLSNSEFSDAEFFRQQVAAIADYVGKF
                     PPEERDLRALAWIEANARQYRQQWQKHVAVNRLAMTRCADCPLAGGDKQIPCAIHSRW
                     LALLRRYAADELSSHEYVTKTLVLLTTHKDWLRVSEARHFLPSPRQAVLNPEAEAAAL
                     D"
     gene            complement(3340..4776)
                     /locus_tag="PLL14_00680"
     CDS             complement(3340..4776)
                     /locus_tag="PLL14_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG02458.1"
                     /translation="MAVAEMTLGDASLITLTFLFTDIEGSTSLWERESEAMRFSLQRH
                     NVLLSSAIERHRGCVFKTVGDAFCVTFDDAADALRAACQIQTSLAAEKWPTRASLRVR
                     VALHTGPAYLSHGDYFGTTVNRCARLLAVTRGGQTLLTSATQALVRESLSSGTSLRDT
                     GMLRLRDLPHPVHVFQLLHPGLSPSLLDISDTSGGNKLTAEPMARFKPIDVYEVPTLP
                     AIVMQALRLLQDPNTDAKAVERVIVHDPAISAKILRVANSVFFGFSRRVSTIAEAVRV
                     LGFTNVQGMLISVGAFDAFRTERLNLRDFWKHSIATATAARFLSPQVHCAADEAFMAG
                     LLHDIGKLIFAVQAESAYQRVLELQHGSALGSLEAERTLFEFTHPEVGEMVAERWDLP
                     ARYVAGIAHHHDPVAAGDEQPFCAVIGLSDQAAHAALCVQGPSGTHDEERTALLETLN
                     LSPKIWDDCLRHLREAQPNIEAFVGAIH"
     gene            5187..>6568
                     /locus_tag="PLL14_00685"
     CDS             5187..>6568
                     /locus_tag="PLL14_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOG02459.1"
                     /translation="MPQSPFGTLSRFMPSGQAEAWFYSLPALIAADIGRVARLPVSLR
                     IVLEALLRHCDGQRVTERHILDLAEWQPRAVRNEEVPFVVARVVLQDFTGVPLLCDLA
                     AMRHVAQARGLDPRRIEPLVPVDLVVDHSVQVDYYGTSQALQQNMAREFERNRERYQF
                     MKWGMQAFETFRVVPPGVGIVHQVNLEHLFHGVRQQAIGSGRLCFPDTLVGTDSHTTM
                     INGVGVVGWGVGGIEAEAAMLGQPVYFLTPDVVGVELSGRLREGVTATDLVLTVTELL
                     RREKVVGTFVEYFGEGAGSLSVTDRATLANMAPEYGATMGFFPVDEKTVDYMRATGRS
                     EEDCVLFEAYFRAQGLFGMPRADQIDYSRSLRLDLSTIVPSLAGPKRPQDRIELPAMA
                     SRFDALLSAPEAADGFDRPAASLGQRYPTEREGIDLGHGDVLIAAITSCTNTSNPAVM
                     IAAGLLARKAV"
     assembly_gap    6569..6668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6669..7760
                     /gene="acnA"
                     /locus_tag="PLL14_00690"
     CDS             <6669..7760
                     /gene="acnA"
                     /locus_tag="PLL14_00690"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767491.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOG02460.1"
                     /translation="EARIHPNLRANYLASPPLVVAFAIAGRVNIDLDSEALGVGSDGR
                     EVYLRDVWPTSEEINAVLPFALDPETFRRRYADVTADQELWKAVPAPVGEVYNWPPST
                     YIACPPFFELPEELAGDIRGARLLLLLGDSVTTDHISPAGSFGEATPAGHWLREQGVS
                     RAEFNSYGARRGHHEVMMRGTFANVRIRNLMLPVDAAGRRPEGGYTLLDGQQTSVFAA
                     AMSHRQRGTPTIVVAGEEYGTGSSRDWAAKGTRLLGVRAVIARSYERIHRANLVGMGV
                     LPLQFVGDDSAASLDLRGDETFDILGLGPGLLPMQKLKLVVERADGQRFERQLLCRID
                     TPIEVRYYRSGGILPFVLEELLGERTANH"
     gene            complement(7843..9090)
                     /locus_tag="PLL14_00695"
     CDS             complement(7843..9090)
                     /locus_tag="PLL14_00695"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG02461.1"
                     /translation="MTIAPNILALVLAGGEGTRLHPLTQHRSKPSVPFGGRFRIVDFV
                     LSNLVNSKIFAIYLLVQYKSQSLIEHIRRSWVLAPIFPEQFIAVVPPQMREGPEWFQG
                     TSDAVYQNLGLIEKRSPDLVVVFGADHLYRMDVRQMVAFHLQHAADATIAALPVPIGM
                     ASAFGIIDAGDDGCVRGFREKPPDPTPMAGDPTRAFASMGNYIFTTEILSTALREGHR
                     LGEKDFGKDLLPRLIQTKRVFAYDFSGNRVPGTRDYEERGYWRDVGTIDAYFAAHQDL
                     LGAEPKFDIFNPRWRIGSSNYQGPSPKFVHAEVDNSIISSGGLIRGARVRNSIVRREV
                     LMEEDVELDECIVMDYAVLRKGVRLRRVIVDRYNTINAGERIGYDMDADRRRFTVTDS
                     GIVVVPRGVGTDTRAEEMAFRYL"
     gene            9421..10590
                     /locus_tag="PLL14_00700"
     CDS             9421..10590
                     /locus_tag="PLL14_00700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG02462.1"
                     /translation="MPSPLMISSIPAANAVAREAWSRLCPVGHPFLNADFLAIIERHG
                     AAAPAWGWTPRHLVASDERGAVVGLLPLYGKTHSHGDFIYDWAWAAAYRQIGRQYYPK
                     LMSCIPHTPVAGPRILVGQGPDAVAIRQALIDGARMLAIRSGASSWHVALPGDDEIPL
                     LERAGLLLSHDVQFHWEDPGCGDFDGYLATFSAAKRRKVRAERRQVQDSGLSVETRHG
                     DQIEPAEWPLLHALYTSTFEKFNNHAAFSAACFADLGANLGRRMVAFIARDRGLPVAV
                     ALCFRSDEALYGRYWGCREHYPSLHFELCFYQGIAYCLREGLRRFEPGAGGEHKLARG
                     FTPIVVHSAHWIADPYMRQVLDRHLALQGGQVADYREQAAAHLPFRRDDSPPSAG"
BASE COUNT         1826 a         3238 c         3502 g         2019 t
ORIGIN      
        1 accagcggcc aggcgagagc ggcgcccgtt ccctcgccga ggcacaggcc gaggtcgagc
       61 agtggttcgg cctgcaacgc ccgcagttgg acgacatggc ccggttccgc cgagcggtgg
      121 cagaagatgc agtagtcgcg aagcgatggc gcgatggcgg tggccaccag caacgccgag
      181 gtgacgatga agccatcgat cagcagcagt atcccgcgct cggctgcggc gagcatcgcg
      241 ccagccatca tcgccatctc gaagccgccg aactcggcga gcaccagcaa cgcatcggca
      301 tcgggcggaa gtgccgcgcg gcccaccgcc tgcgccagca aggtccgttt gcgtgcaagt
      361 ccgacggtgt cgaggccggt gccccgcccg acgcagtcgg ccaggggtgc gccggtcagt
      421 tgctgcgtca gcagcgacgc ggcggcggta ttgccgatgc ccatttcgcc gaaggccatt
      481 acccggcaac cctggtcggc caggccgcgg gcgatggcgg cgccgcgtgc cagcgccagg
      541 tcgcgctctg cggcgctcat cgccgcctgt tcgatagcgt tgtgcgtgcc gaaggcgatc
      601 ttggcgtcga ccaggccatc gcgcgcgccg aaatcgtgcg ccacgccagc gtctatcacc
      661 tgcagctgga ggccgttgtg gcgggcgaaa acattgattg ccgcgccacc agcgagaaag
      721 ttctcgacca tctgccaggt cacttcctgc gggtacgccg agacaccggc gcgggcggcg
      781 ccgtggtcgc cggcgaagac gagcagatgc gggtgatcga gactcgggct cagtgactgc
      841 tggacctggc cgatctgcag ggccaggcgt tcgagctggc cgagtgaacc gggtggcttg
      901 gtcttgccgt cgatccgggc ctgcaggccc ggcatggttg ccggtggcga gatggagaaa
      961 tgcatgcgct gacaggcctc ggcaaaagcc gcagattata cgcatcgccg gccatgctaa
     1021 gctgtgcggg atgcgagaac tgatccttgg tggcgcccgt tcgggcaaga gtcgattggc
     1081 cgagatccgt gccactgaaa gcggcctgcg ggtcgtttac gtggcgacgg cactgcccct
     1141 cgacgccgag atgtcgcggc gtatcgaaca tcatcgcgca aggcgctgtc ccgactgggg
     1201 gctggtcgtc gagccgctcg atctggccgc tgcattgcgt cggcacgcgg ctgccgacgt
     1261 ctgcctgctg gttgactgcc tgacgctgtg gttgtcgaac gtgttgttct ccggcgaagc
     1321 cgcctgccag gccgaagccg gcgaagcggt gtgctgccag catctggccg gcgcgacgca
     1381 agccctgatc gatctcctgc cgcaactgcc cgggcacgtc atcctggtct ccaacgaggt
     1441 cggctgcggc atcgtgccga tggcggcact gtcacgcctc tttgccgacg agcaggggcg
     1501 cctgaaccag cgggtggcgg cggtatgcga tcgggtgacg ctggtggcgg caggcttgcc
     1561 gctgaccctc aagtaagtag tgcccgctgt tcgcgaccgg gcgatcgatg ctattgttga
     1621 actggtctga caactttgcc cagagaggag ccgagacaaa tgtacaagcg aatcatggtt
     1681 gccgtggatg agagcttcat gaccgaccag gtcctgcacg cggcgatcga actggcccag
     1741 gtcacggggg cgaagctggc gatctgtcat gcgatcgacg agacgattct tgcccaacgt
     1801 gaagtggcga tgatgttgcc gaacagcgtc ggcaagaccg aggcccgctt gcgcctcggt
     1861 gctcaggctt ttctcgaccg cctggcagca acggcgcgtg ggttgggagt cgacgccgag
     1921 gtcaagatcg tcgaatcgga gcagaagcat gtttcggaca tgctgatcga ggccgcagcc
     1981 gagtggcagg ccgacttgct ggtggttggt acgcacggcc ggcgcggtat cgaacgttac
     2041 ttcgtgggta gcgcagccga gcgtttggtg cgcaaggcgc agctttcgct cctgttggta
     2101 cgcggcgaag aaccggacga gtagttcgcc agggcgccgg ttcctgcgca gctgaccatc
     2161 cctcggtaaa gcggcccctc tagtcaaggc aagaatcaag tttgttgagg ctgagctctc
     2221 gacttcagta gcagttggac ggcgctgccc cggttttgcc agcgccctgg tttgagctgt
     2281 ggctgtggat cttgctattg ctggggttgg gggagggacg tgctggttgt ctctattgcc
     2341 gaggactgca tattccttcc tttctccgcc agcaggccgg cttttgtaac gccgatatgt
     2401 ccatgcgctg agaaccgaag atctcttgcg caagagccca acgggcacgc tctgcccgag
     2461 aaacgcctgc catttgctca agccacttct ggatgcgatt catggtaagc aagtcctcca
     2521 aaggaatgaa ttgatcagga tccgcagcaa cgtgtgcaat actaatcaaa ggctgtataa
     2581 atgtacagca cattttgttc atcgcttcgg cacgacgttg attcaatcga tctgcagcga
     2641 ctgattggca ggatcggaat cacagtctgg cgtgaggttg tcgtattgct gcaccgcaac
     2701 aaattgtgtt acagtaaact tgtttgctgg ttgtgcgccg atgctgccgc tgaaggtgtg
     2761 gatatgtaca tcgttgatga tccgacgctg gcgctgatcg cccgttttgt cggtggcctg
     2821 tcgaattccg agttttccga tgcggagttc tttcggcagc aggtggccgc catcgccgat
     2881 tatgtcggga aatttccgcc ggaagagcgg gacctgcggg cgttagcctg gattgaagcc
     2941 aacgccaggc aatatcgtca acaatggcag aagcatgtag cggtcaatcg gctggcgatg
     3001 acccgctgcg cggattgtcc gctggccggt ggcgacaagc aaattccctg tgccattcat
     3061 tcccggtggc tggcgttgtt gcggcgctac gccgcggacg aactctcttc acacgagtat
     3121 gtgaccaaga ctctggtctt gctgactacc cacaaggact ggctgagggt gagcgaagcc
     3181 cgccatttcc tgccgtcccc gcgccaggcg gtgctcaacc ccgaggccga agcggcggcg
     3241 ctggactagg cgccagactc tcaccgcgga gcggtttgcc acccggtggc cgtcgtcgaa
     3301 gacgcgtcgg gccggcacgg gacggcgtcc cagtcaatct cagtggattg ccccgacaaa
     3361 ggcctcgatg tttggttgcg cctcgcgtag atgccgaaga cagtcatccc aaatcttcgg
     3421 gctcaggttg agggtttcga gcagggctgt tcgttcttcg tcgtgcgtgc cagacggacc
     3481 ctggacgcag agggcggcgt gcgccgcctg gtcggaaagg ccgatcaccg cacagaacgg
     3541 ctgctcgtcg ccggccgcaa ccggatcgtg gtggtgggcg atgccggcaa cgtacctggc
     3601 cggcagatcc cagcgttcgg caaccatttc gccgacttcg gggtgggtga attcgaacag
     3661 tgtcctttcc gcctcaagac tgcccaaagc ggatccatgc tgaagttcga ggacccgttg
     3721 gtaggccgat tccgcctgga cggcgaagat cagtttgccg atgtcgtgca gcaggccggc
     3781 catgaacgct tcatcagcgg cgcaatgcac ctgcggggaa aggaaacgtg cggctgtcgc
     3841 cgtggcgatc gagtgtttcc agaaatccct caggttcagc cgctcggtgc ggaaggcgtc
     3901 gaaggcgccg accgagatca gcatgccctg cacgttggtg aagccgagaa cgcgtacggc
     3961 ttcggcgatg gtcgagacgc gtcgggagaa accgaaaaac accgagtttg ccactcgcag
     4021 gatcttggcc gagatcgccg ggtcgtgcac gatcacccgt tccacagcct tggcatcggt
     4081 gttgggatcc tgcagcaacc tgagcgcctg catgacgatc gccggcaggg tcgggacttc
     4141 gtaaacatcg attggcttga agcgggccat cggttcagcg gtaagcttgt tcccgcccga
     4201 ggtgtcgctg atgtccagca ggctgggcga cagcccggga tgcaggagct ggaagacatg
     4261 caccgggtgc ggcaggtccc gcaggcgcag catgccggta tcgcgcaggc tggtgcccga
     4321 ggacagggat tctcgcacca gggcctgggt cgccgaggtc agcagcgtct gcccgccgcg
     4381 ggttaccgcc agcaagcggg cgcagcggtt gactgtcgtg ccgaaatagt cgccatggct
     4441 caggtacgcc ggaccggtgt gcagcgcgac gcgcacccgc aggctggctc gtgtcggcca
     4501 cttctctgcg gcaaggctgg tctggatctg gcaagctgct ctgagcgcgt cggcggcgtc
     4561 gtcgaaagta acgcagaaag cgtcgccgac agtcttgaag acacagcccc ggtggcgctc
     4621 tatggccgag gagagcagca cgttatggcg ttgcagactg aagcgcatcg cttcggactc
     4681 gcgctcccaa aggctcgtcg agccctcgat gtcggtaaac aggaaggtga gcgtaatcag
     4741 cgacgcatcg cccaaggtca tttctgctac agccattcgc agtctcgctc gttcatccgt
     4801 ggggaatcgt cggctgccgc ccgatgatcg tcgtgcggcg ggggaatatg agattcaaca
     4861 cacgagagta tcccggcttc tggcggttgc cgtcaatcgc cgattgtcgg caaccgccgc
     4921 gttggcaagg gtcgagatcc gtacgttgct cgtggtcggc aaccgctggt tgtctgggca
     4981 agcataaaat cgcgaggtga gcgtcgaccg catcgcgtcg ccggtgcggc gacaagtccg
     5041 cgcagttggc cgtttccctg gacagcccga atcgcgttgc ggcgtggccc gcaaagtgcc
     5101 gcaagccggg gaacgagcag cgctgccctg gtgtcacttg cgtatccacg cgaagtgcgg
     5161 acctgttacc tgttcggagg cctgtgatgc cccaatcgcc attcggtacc ttgtcgcgtt
     5221 tcatgccttc cggtcaggcg gaggcctggt tttattcatt gccggctttg attgccgccg
     5281 atatcgggcg ggtggcgcgc ctgccggtgt cgctgcgcat cgttctcgaa gcgctcctgc
     5341 ggcattgcga tggccagcga gttaccgagc ggcatattct cgacctcgcc gagtggcagc
     5401 cccgagcggt acgcaacgag gaggttcctt tcgtcgtcgc acgcgtcgtg ctgcaggatt
     5461 tcaccggtgt accgctgctt tgcgacctcg cagcgatgcg gcatgtggcg caggcgcgtg
     5521 ggctcgatcc gcggcgtatc gagccactgg tgcccgtaga cctggtcgtc gaccattcgg
     5581 tacaggtcga ttactacggc acgtcgcaag ccctgcagca gaacatggcg cgggagttcg
     5641 agcgcaaccg tgagcgctac cagttcatga aatggggcat gcaggcattc gaaaccttcc
     5701 gcgtcgtacc gcccggcgtc ggcattgtcc atcaggtcaa tctcgaacac ctgttccatg
     5761 gcgtgcgcca gcaagccatt ggcagcgggc ggctgtgttt tcccgacacg ctggtcggca
     5821 ccgattcgca caccacgatg atcaatggcg ttggcgttgt tggctggggg gtcggcggca
     5881 tcgaggccga ggcagcgatg ctcggtcaac ccgtgtattt tctgacgccc gacgtggttg
     5941 gcgtcgaatt gagcggtcgt ttgcgcgagg gcgttaccgc caccgatctg gtgctgacgg
     6001 tgaccgaact gttgcgccgc gaaaaggtgg tcggcacgtt cgtagaatat ttcggcgagg
     6061 gtgccggcag cctgtcggtg accgaccgcg cgaccctggc caacatggcg ccggaatacg
     6121 gggcgacgat gggcttcttc ccggtcgacg agaagaccgt cgactatatg cgcgcaactg
     6181 gtcgcagcga agaggattgc gtgctcttcg aggcctattt ccgtgcgcag ggcctgttcg
     6241 gcatgccgcg tgccgatcag atcgactact cgcgcagcct gcgcctcgat ctgtcgacca
     6301 tcgtgccctc gttggccggc cccaaacggc cgcaggaccg gatcgagctg ccggccatgg
     6361 ccagtcgctt cgatgccttg ttgagcgcgc ccgaagccgc agatggcttt gaccgtccgg
     6421 ccgcgagcct tgggcagcgc tacccgaccg agcgggaggg catcgatctc ggccacggcg
     6481 acgtgctgat cgcggcgatc acgtcctgca ccaacaccag caatccggcg gtgatgatcg
     6541 ccgccggcct gctcgccagg aaggcggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnga agcgcgcatc catcccaacc tgcgcgccaa ttacctggct tcgccaccgt
     6721 tagtggtggc ttttgccatt gccggccggg tcaacatcga cctcgacagc gaagcactgg
     6781 gagtcggcag cgatggtcgg gaggtctacc tgcgtgacgt ctggccgacg tcagaagaaa
     6841 tcaacgccgt gctgccgttc gcactcgacc ctgaaacctt ccgccgccgg tacgccgacg
     6901 tgaccgccga ccaggaactg tggaaagccg ttcccgcgcc cgtcggggag gtctacaact
     6961 ggccgccatc aacttacatc gcctgcccac cattcttcga actgcccgaa gaactggcag
     7021 gcgacatccg cggggcacgt ctgctgttgc tgctcggcga ttcggtgaca accgaccaca
     7081 tctcgccggc cggctcattc ggcgaagcga caccggccgg tcactggctc agggagcagg
     7141 gcgtcagccg tgccgaattc aattcctacg gcgcacgccg tggccaccac gaggtgatga
     7201 tgcgtggcac tttcgccaac gtccgcattc gcaatctgat gctgcccgtc gatgccgcgg
     7261 ggcgccggcc ggaaggcggc tacacgctgc tcgacgggca gcagacaagc gtctttgccg
     7321 ccgcgatgag ccaccgtcaa cgcggcacgc cgaccatcgt cgttgccggc gaggagtatg
     7381 gcaccggttc gtcgcgcgac tgggcagcca aggggacgcg tctgctcggg gtgcgggcgg
     7441 tgattgcgcg cagctatgaa agaattcacc gtgccaacct ggtcggcatg ggcgtgctgc
     7501 cgttgcagtt cgtcggtgac gattcggcgg cgtcacttga cttgcgcggc gacgaaacct
     7561 tcgacatcct tggcctcggc ccggggctgc tgccgatgca gaagctcaag ctggtggttg
     7621 agcgtgctga cggtcagaga ttcgagcgcc aactgctgtg tcgcatcgac acgccgatcg
     7681 aggttcgcta ctaccggtct ggcggtatcc tgcccttcgt gctcgaagaa ctgcttggtg
     7741 agcgcacggc gaaccattga gcggcgctgc ggacggtgcc tgggagcacc ggcgcattgg
     7801 gaaaacggca tggtcggctc ggatggcaag tgccggccgc gttcagagat agcggaatgc
     7861 catctcctcg gcacgggtat ccgttcccac ccctctgggt acgaccacga tacccgagtc
     7921 ggtgacggta aaccgccgcc ggtcggcgtc catgtcgtag ccgatccgct ctccggcgtt
     7981 gatcgtgtta tagcggtcga cgatgacgcg cctgagtctg acgcccttgc gcaggacggc
     8041 gtagtccatg acgatgcact cgtcgagttc gacatcctct tccatcagta cttcgcgacg
     8101 aacgatcgaa ttgcggacgc gagcccctct gatcagaccg ccggagctga tgatgctgtt
     8161 gtccacttcg gcatgcacga acttcggcga tggtccctga tagttgctgg agccgattcg
     8221 ccacctggga ttgaatatgt cgaacttcgg ttcggcaccc aggaggtcct ggtgggcagc
     8281 gaagtaggca tcgatcgttc cgacgtcgcg ccaatagcca cgttcctcgt agtcgcgtgt
     8341 tcccggcacg cgattcccgg agaagtcgta ggcaaacacc cgcttggttt gaatcaggcg
     8401 cggcagcaga tccttgccaa agtccttctc tcccaggcgg tgaccctcgc gcagcgcggt
     8461 gctcagtatc tcggtagtga aaatgtagtt gcccatcgac gcgaaggcgc gtgtgggatc
     8521 gccggccatg ggagtgggat cgggtggttt ttcccggaag ccacgcacgc aaccgtcgtc
     8581 gccggcgtcg atgatcccga atgccgaggc catgccaatc ggcaccggca gagctgcgat
     8641 ggtcgcgtcg gcagcgtgtt gcaggtggaa ggcaaccatc tggcggacat ccatgcgata
     8701 gaggtggtcg gcgccgaaga ccacgaccag gtccggagaa cgcttctcga tcagtccgag
     8761 gttctgatag accgcgtcgg aggttccctg aaaccattcc gggccttccc gcatttgcgg
     8821 tggaaccacg gcgatgaact gctcggggaa gatcggggcc agtacccagg agcggcggat
     8881 gtgttcgatc agcgactgcg atttgtactg gaccaggagg taaattgcga agatcttgga
     8941 attgaccagg ttggaaagca cgaagtcgac gatgcggaag cggccaccga aaggaacaga
     9001 cggcttggag cggtgttggg tcagcggatg cagacgggtg ccttcgccgc ctgccaggac
     9061 cagagccaga atattgggcg cgatggtcat ctcctcgttt gtccctgtaa gacagtaggg
     9121 atcgcgtgct gccgctgcaa ggcagtgacc agcgcgccgc cgcatcgccc ttgccaggat
     9181 ccgcacaagt cggttcgagc gtttgttgaa gttaaactcg accatgcgat catcgggaag
     9241 ttccttccgt tccccgtcgc ggcgcgattg ttgcgtgtca ggcatgcgct atcgcgctcg
     9301 accggagcgc agttgtcatt ctgcttcgcg ctgtggcaac atcaggcgta tgcagcgccg
     9361 ttggcccgat cccgttcgcg ccgatcccct ggcgctgcac caatcagccc ggagaccggc
     9421 ttgccgtcgc cactcatgat cagcagtatc cctgccgcca atgcggttgc gcgcgaggca
     9481 tggtcccgcc tttgcccggt cgggcatcct ttcctcaatg ccgattttct ggcaatcatc
     9541 gagcgccacg gggcggcggc gcccgcctgg gggtggaccc cccggcatct ggtggcaagc
     9601 gacgaacggg gtgcggtcgt cggtctgctg ccgttgtacg gcaagactca ttcgcacggc
     9661 gatttcatct acgactgggc gtgggcggcg gcttaccggc agatcggccg ccagtattac
     9721 ccgaagttga tgagctgcat accgcacacg ccggttgccg ggccgcgcat cctggtcggg
     9781 caagggccgg acgccgttgc catccggcag gccctgatcg atggcgcgcg catgctggcg
     9841 attcgatccg gcgcttcctc gtggcacgtg gcgttgcccg gtgacgacga aatccctctg
     9901 ctggagcgtg caggactgtt gctcagccac gatgttcagt ttcactggga ggatcccggc
     9961 tgcggtgact tcgatggcta tctggccact ttcagtgccg ccaagcggcg caaggtcagg
    10021 gccgaacggc ggcaagtcca ggacagcggc ttgtccgtcg agacccgcca tggggatcag
    10081 atcgagcccg ccgaatggcc gctgctgcac gcgctttaca ccagcacctt cgagaagttc
    10141 aacaatcacg ctgcgttttc tgcagcctgc ttcgccgatc tcggggcgaa cctcggtcgc
    10201 cggatggtgg ccttcatcgc ccgcgaccgc ggtctgccgg tggccgtcgc tctctgtttc
    10261 cgcagcgacg aagcgctcta cgggcgctat tggggttgcc gcgagcacta ccccagcctg
    10321 catttcgaac tctgcttcta tcagggcatt gcgtattgtt tgcgcgaagg cctgcggcgc
    10381 ttcgaacccg gcgccggcgg cgaacacaag ctcgcacgcg gcttcacgcc gatcgtggtg
    10441 cactcggcgc actggatcgc cgacccgtac atgcgccagg tgctggaccg gcacctggca
    10501 ttgcagggtg ggcaggtcgc cgactaccgc gagcaggcgg ctgcgcatct gccgttccgg
    10561 cgtgatgact cgccgccgtc agcaggttga ggcagcgttg agggattgcc accatgccgg
    10621 tttcgctgcg cgcagccgag gataccgcaa cggatatcca ggcgatgctg actctggtca
    10681 gcctg
//
LOCUS       DAOSIT010000012        10232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6378_length_10232_cov_9.286135, whole genome shotgun sequence.
ACCESSION   DAOSIT010000012 DAOSIT010000000
VERSION     DAOSIT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_10232_cov_9.286135"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLL14_00705"
     CDS             <1..265
                     /locus_tag="PLL14_00705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG02463.1"
                     /translation="NAGVRLTLAPTAGCSLATLPSAVGGAVELLVGPEGGLAPEEVRL
                     AALTGFVSVRLGPRVLRTETAGLAALAAIQFLWGDLKEETTDV"
     gene            258..1943
                     /gene="pap"
                     /locus_tag="PLL14_00710"
     CDS             258..1943
                     /gene="pap"
                     /locus_tag="PLL14_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOG02464.1"
                     /translation="MFESAELGHKVDKETFRVEEPKLRAALLETQEQLRENGSFPVII
                     LISGVDGSGKGETINILYEWMDPRYLSTHAFYAPTAEERARPSMWRYWRALPPKGRIG
                     IFSGSWYSHPIAERISNSLSRSDFDQRMDQLNRFEAMLVNEGALVLKFWIHLSKDAQR
                     ERLKSIEANPLTRWRVTKASWDRLKTYDKLQEVAGHLLRTTNTPWAPWIIVEGTDDRY
                     RSLTVGRTILDSLQKRLAEKREQSSPVAPPLSPRIDRLDVLSSLDMSQSLNKKAYETE
                     LATWQGRLAELMRHPEFKKRALILAFEGSDAAGKGGAIRRVMSAMDARQYQIIPIAAP
                     TEEERAQPYLWRFWRHMPRLGRVAIFDRTWFGRVLVERVEGFCSEADWLRAYSEINDF
                     EHDIWRAGGIVIKFWLQISDEEQLKRFEERAQIEHKRFKITEEDWRNREKAGAYHTAV
                     CDMVDRTSSGTAPWTIVESNDKLFARIKVLRTICERLEKELKVDPMKEARVLMPRAAK
                     AEAQKEAKTEGPPPARDAQDAKAHKAKALHVEKEKEAKDPKAAKAGEKDRPAV"
     gene            1945..3264
                     /gene="argA"
                     /locus_tag="PLL14_00715"
     CDS             1945..3264
                     /gene="argA"
                     /locus_tag="PLL14_00715"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOG02465.1"
                     /translation="MADAVSAAQFVAWFRSVAPYVNLFRGKTFLIAFGGKAIAGQLAR
                     TLAYDVNLLAALGVRLVLVHGARPQIEALLRQRGLQSVYHGNYRVTDAQAIDCVIDAV
                     GSIYVEIEALLSQGLADTPMANSTIRVVGGNFITAKPVGVVDGVDLQYSGQVRKIDAE
                     GIRAQLGLGNIVLLSCIGSSPTGEIFNLAMEEVAEAVATALLADKLIFLSDSRGATDI
                     DGALLDELTVEAAERLISLGDWLSTDLKRYLPCAVCASRAGVGRVHLIGYDEDGTLLR
                     ELFTRDGVGTVVTRESLEQLHDARPDDIGGLVALIEPLEEDGTLVRRSRELLEREITR
                     FSVVEHDGVIVGCAALYPYGEGQAELACLAVHPHYRRWGYGALLMKHIEAQARASGVK
                     RLFVLTTVTSQWFKERGFTVRQIDELPAEKRLVYNLQRRSSVLMKEL"
     gene            3333..3605
                     /locus_tag="PLL14_00720"
     CDS             3333..3605
                     /locus_tag="PLL14_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOG02466.1"
                     /translation="MTRMVNCVKLGRAAEGLDFPPYPGELGKRVYEAVSKEAWQQWVR
                     MQTMIINENRLNLADAAHRKYLAEQMERHFFGGGADKVQGYVPPGA"
     gene            complement(3668..4330)
                     /gene="rpiA"
                     /locus_tag="PLL14_00725"
     CDS             complement(3668..4330)
                     /gene="rpiA"
                     /locus_tag="PLL14_00725"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOG02467.1"
                     /translation="MTQDELKKAVGRAAIAHVVEGGIVGVGTGSTANFFIDALAEIRH
                     RIRGAVASSEATRKRLEGHGIEVFDLNDVDQIPVYVDGADEINAAMQMIKGGGGALTR
                     EKIVAAVAGKFVCIADASKLVPVLGRFPLPVEVIPMALGHVQRELSRIGGSPRLRANF
                     VTDNGNRIVDVHALRIEDAVALETRLNQIVGVVTNGLFACRPADICLLATGAGVQTLT
                     AT"
     gene            4519..4893
                     /locus_tag="PLL14_00730"
     CDS             4519..4893
                     /locus_tag="PLL14_00730"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOG02468.1"
                     /translation="MADESPFKGQTGIRRIWNALHFSGAGLRAAYAAEDAFRQEVLLA
                     ALLIPLAFFLSPNGLGRALLVGSVLLVLIVELLNSAIEATVDRISMERHPLAKRAKDI
                     GSAAVLLALFNVLAIWACVLIG"
     gene            complement(4975..5874)
                     /gene="ftsX"
                     /locus_tag="PLL14_00735"
     CDS             complement(4975..5874)
                     /gene="ftsX"
                     /locus_tag="PLL14_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOG02469.1"
                     /translation="MSAWLAQHLAALRDALRRLAAAPLNSLLSLLVIGVALTLPTAGW
                     LTLDNLRKLTGETPGVQQISIFLALDAGKREHSEIESRLKEVRAGPWRFVPREEALKR
                     LQASEGMSEIIASLPRNPLPDAYIITPTEAQPETLESLARIFSTWPKVAHVQLDSAWV
                     KRFDALLRLGKLVVILLAVLFAGALVTITFNTIRLQILAQAAEIELAKLIGATDTYIQ
                     RPLHYFGLLQGALGGLCAALLVSAGFQLLTPPVAELARLYGANFALQGLSVSDILVLT
                     AIGGALGWLGAKISVLIHLRRII"
     gene            complement(5871..6557)
                     /locus_tag="PLL14_00740"
     CDS             complement(5871..6557)
                     /locus_tag="PLL14_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG02470.1"
                     /translation="MITACDVTKRYPEGREALRKVSFEITAGEMVFLTGHSGAGKSTL
                     FKLLAAIERPTSGSIVINGQNLASLRRNAIPYLRRKLGLIFQDRKLLFDRTVLDNVLL
                     PLAIVGHPAREAMRRAQAALAKVGLPNREKAFPIALSGGEQQRLAIARAVVNRPAIIL
                     ADEPTANLDAASAGEILEIFRAFQQVGVTVLIATHDPLHLPHLQPRILRLDQGEMSDA
                     QAQSTEGNTA"
     gene            complement(6554..7546)
                     /gene="ftsY"
                     /locus_tag="PLL14_00745"
     CDS             complement(6554..7546)
                     /gene="ftsY"
                     /locus_tag="PLL14_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG02471.1"
                     /translation="MFGFLKKQPGQKPTPTTPADTEVAVNAAAVPWRERLKAGLARTR
                     AQFGGKLKLLFSRGKVDDELLEDLEALLLGSDVGLDATQHLLYHLKVRAKRDKLDTPQ
                     AIQRALSEILLELLQPLEEPLDLASHRPFIIMIAGVNGAGKTTSIGKLAKHFQAQGLS
                     VLLAAGDTFRAAAREQLQTWGERNNVSVVSQLSGDPAAVIFDAIAAARARGIDIVLAD
                     TAGRLPTQLHLMDEIAKVRRVIQKADPSGPHETLLVLDATFGQNTLQQVMAFDKAIQV
                     TGLVLTKLDGSAKGGVVAAIARQCPKPVRFIGVGESIDDLRPFVARDFVDAMFE"
     gene            7642..9009
                     /locus_tag="PLL14_00750"
     CDS             7642..9009
                     /locus_tag="PLL14_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG02472.1"
                     /translation="MPMRHARLIPLLLALAAPAPVLAETYEHQLANGLRIIVKEDRRA
                     PTAAHMVWYRAGSMDETNGTTGVAHVLEHMMFKGTPSAGPGEFNRRVAAAGGRDNAFT
                     GHDYTAYFQQVPNQKLSEMMQLEADRMRHLTLDAQEFAQEIRVVMEERRLRTEDQPQA
                     LLFEQLSAVAFQAHPYRVPVIGWMNDLENMTVNDARAWYEHWYAPNNAYVVVVGDVDH
                     QTVFAAAEQYYGPLPARPLPGRKPQDEPVQTGIRRLTVKAPAELPVVMMAYKVPVLRD
                     VDQDVEPYALEMLSAVLAGHEAARFTNHLIRQQRLAVDATASYDSTRRGPGVFHLYGS
                     PSEGKTRADLESGLRAEIALVQAQGVTPDELARAKAQLIAGQIYKLDSIFAQAMEIGQ
                     LESVGIPNAQNRRMIEKLQAVTAEQIQAVARKYFRDEQLTVAELDPQPLPTAPRARSP
                     FAPRH"
     gene            9020..>10232
                     /locus_tag="PLL14_00755"
     CDS             9020..>10232
                     /locus_tag="PLL14_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG02473.1"
                     /translation="MNPSMLLIGLCLALFAQLSHAGPKIERWQTTSGARVLFVENHSL
                     PIVDVQVDFAAGTAQDGEGKAGVASLTRGILDLGVPGMDETQIASSMADIGALLSGGV
                     DMDRASVTLRTLSMADKRGPALEMLRAILSQPQFPAAVFDREQARSISALKEALTRPD
                     AIASKAFWAAMYVAHPYGRYATPESVSSLARADVVAFHAAHYTAQRATVAIVGDLSRA
                     QAESVAEQLTVGLPSGPAMAGVVLPKLPSGGEQRIAHPAVQAHLLLGMPALRRGDPDF
                     FPLLVGNYSLGGGGFVSRLMKEVRDKRGLAYSVHSYFLPLQQLGPFQIGLQTKKAQAN
                     DALKVTREVLATFLAEGPSETELQAAKQNLIGSFPLRLDSNRKILENVALIGFYDLPL
                     DYLDRYPENIEK"
BASE COUNT         1906 a         2962 c         3388 g         1976 t
ORIGIN      
        1 gaacgcaggt gtgcgcctga ccctggcgcc cacggcggga tgttctctgg cgacgctacc
       61 gtcggcggtc ggcggcgcag ttgaactgtt ggtcggcccc gagggcgggc tggcacccga
      121 ggaggtgcgg ttggcggcgt tgaccggttt tgtatcggtt cgccttgggc cgcgcgtcct
      181 gcgtaccgaa acggctggcc tcgctgccct ggcagcaatc cagtttttgt ggggtgactt
      241 gaaggaggag actactgatg tttgaatcag cagaacttgg gcacaaggtc gacaaggaaa
      301 cgttccgggt cgaggaaccg aagctgcgcg cggcgctgct cgaaacgcag gagcaattgc
      361 gcgaaaacgg cagctttccg gtgatcattc tgatcagcgg tgtcgacggg tccggcaagg
      421 gtgaaacgat caacatcctc tatgagtgga tggacccacg ttacctctcg acgcatgcct
      481 tttacgcgcc gaccgctgaa gagcgcgccc gtccgtcgat gtggcggtac tggcgtgcgc
      541 tgcccccgaa agggcgaatc gggatctttt cgggttcgtg gtattcgcac ccgatcgccg
      601 agcgaatcag caacagcctt tcgcgatccg atttcgacca gcgcatggat cagctcaacc
      661 gtttcgaggc gatgctggtc aacgaaggcg cgctggtgct caagttctgg attcacctct
      721 ccaaggatgc gcaacgcgag cgcctgaagt cgattgaagc gaacccgctg acccgctggc
      781 gagtgaccaa ggcgagttgg gaccgcctga agacttacga caaattgcag gaagttgccg
      841 gccacctgct gcgcacgacg aacaccccat gggcgccgtg gatcatcgtc gagggcaccg
      901 acgaccgtta tcgttcactg accgtcggtc ggacgatcct cgactcgctg cagaagcggt
      961 tggccgaaaa gcgcgagcag agttcgccgg tggctccgcc gttgtcgccg cgaatcgacc
     1021 gtcttgatgt gctgagttcg ctggacatgt cgcagagtct gaacaagaag gcctacgaaa
     1081 cggaactggc cacctggcag gggcggctgg cggagttgat gcgccacccg gagttcaaga
     1141 aacgggcgtt gattctcgct ttcgagggtt cggacgcagc cggcaagggc ggtgccattc
     1201 ggcgcgtcat gtcggccatg gacgcgcgcc aatatcaaat catcccgatc gccgcgccaa
     1261 ccgaagaaga gcgcgcacag ccctatctgt ggcgattctg gcggcacatg ccgcgcctcg
     1321 ggcgagtggc cattttcgac cgtacctggt ttggtcgcgt gctcgtcgag cgggtggagg
     1381 gattctgcag cgaagcggac tggttgcgcg cctattccga gatcaacgat ttcgaacatg
     1441 acatctggcg tgcaggcggc attgtcatca agttctggtt gcagatcagc gacgaggaac
     1501 aactcaaacg cttcgaggag cgcgcccaga tcgagcacaa acgcttcaag atcaccgagg
     1561 aagactggcg caaccgcgag aaggcaggcg cctatcatac ggcggtctgt gacatggtcg
     1621 accgtaccag ttccggtacc gcgccgtgga ccatcgtcga gtccaacgac aagttgtttg
     1681 cccggatcaa ggtcttgcgg acgatctgcg aacgtctgga aaaggaactg aaagtcgacc
     1741 cgatgaagga ggcgagggtt ctgatgccga gagcggcgaa ggcagaggcg cagaaggaag
     1801 cgaaaaccga ggggccaccg ccagccaggg atgcccagga cgccaaggcc cataaggcga
     1861 aagccttgca cgtcgagaag gagaaagagg cgaaggatcc gaaggcagcc aaggccggcg
     1921 agaaggacag accggcggtg tgagatggcg gacgccgtga gtgctgccca gttcgtggcc
     1981 tggttcaggt cggttgcgcc gtacgtcaat ctgtttcgcg gcaagacctt cctgatcgcc
     2041 ttcggaggca aggccatcgc cggacaactg gcgcgtacgc tggcctatga cgtgaatctg
     2101 ctggcggcgc tcggcgtgcg cctggtgctg gtgcacggtg cacggccgca gattgaagcg
     2161 ttgttgcgcc agagagggct gcaatcggtc tatcacggca actatcgggt taccgatgcg
     2221 caggcgatcg attgtgtcat cgacgctgtg ggcagcatct acgtggaaat cgaggccctg
     2281 ctgtcgcagg gcctcgccga taccccaatg gccaacagta ccattcgggt ggtgggtggg
     2341 aatttcatta ccgccaaacc ggtgggcgtg gttgatggcg tcgatctgca atactccggc
     2401 caggtgcgca agatcgatgc cgagggcatc cgcgcgcagt tggggctcgg caacatcgtc
     2461 ctcctgtcgt gtatcggctc gagtccgacc ggcgagatct tcaacctggc gatggaggag
     2521 gtcgcagagg ctgtggccac cgcgctgctc gccgacaagc tgatttttct gagcgatagt
     2581 cgcggtgcga ccgacatcga cggagccttg ctcgacgagt tgacggtcga agctgccgag
     2641 cgcctgatca gcctgggtga ttggctgtcg acggacctca agcgctacct tccctgcgct
     2701 gtctgcgcca gtcgcgccgg tgtcggccgt gtgcacctga ttggttacga tgaagatggc
     2761 accttgctgc gagaactttt tacccgcgat ggggtgggta cggtagtcac ccgcgagtcg
     2821 ctggaacagt tgcacgacgc ccggcccgat gacattggtg ggctggtcgc cctgatcgag
     2881 ccgctcgaag aagatggtac actggtgcgc cgctcgcgcg agttgctcga gcgcgagatc
     2941 acgcgcttct ccgtggttga gcacgatggc gtgatcgtcg gatgcgctgc cctgtatccc
     3001 tatggcgaag ggcaggccga actggcctgt ctcgccgtgc atccgcacta tcgtcgctgg
     3061 ggctacggcg ccctgttgat gaagcatatc gaggcacaag cgcgtgcttc tggtgtcaag
     3121 cgcctgttcg tgctgactac cgtgacctcg cagtggttca aggagcgtgg ttttacggtc
     3181 cgccagattg acgagttacc ggctgagaaa cgactggtct acaacctgca acgtcgttcc
     3241 agcgtattga tgaaggaact ctagggaccg cttgccaggt agcccgtggg ccggccggcg
     3301 gaaccccgag gagtcattga ggagttgatt gcatgacccg aatggtgaat tgtgtcaagc
     3361 tcggccgtgc ggcagagggc ctggatttcc cgccttatcc cggcgagttg ggcaagcgtg
     3421 tatatgaggc cgtttcaaag gaagcatggc aacaatgggt tcgcatgcag accatgatca
     3481 tcaacgagaa tcgtctcaat ctggccgatg cggcgcatcg caagtatctg gccgaacaga
     3541 tggagaggca tttcttcggt ggcggtgccg ataaggtgca ggggtacgtg ccgcctggcg
     3601 cctgatccgg ggtcgcctct gggttcgccg ggccgttcga gacaccgaac agcgggccgt
     3661 cgggtgttca ggtggcggtc agcgtctgca caccggcgcc tgtagccaac aggcagatat
     3721 ccgccggacg acacgcaaaa aggccgttgg tgaccactcc gacgatctga ttgaggcgtg
     3781 tttccaacgc caccgcgtct tcaatcctga gtgcgtggac gtccacaatg cgattgccgt
     3841 tgtcggtgac gaaattcgcg cgcaggcgtg ggctgccacc gattctagac agttcgcgtt
     3901 ggacgtggcc aagcgccatc gggatcactt cgaccggtag cgggaagcgg ccgagcaccg
     3961 gtaccaactt ggaggcatcg gcaatgcaga cgaactttcc ggcgacggcg gcaacgatct
     4021 tctcacgggt caaggcgccg ccgccaccct tgatcatctg catggccgca ttgatctcgt
     4081 cggcgccgtc cacgtaaacc gggatctggt ctacgtcgtt gaggtcaaat acctcgatgc
     4141 catggccttc gaggcgtttg cgcgtggcct ccgagctggc tactgcgccg cggattcggt
     4201 gccggatttc agccagcgca tcaatgaaga aattggctgt cgagccagtt ccgacaccga
     4261 cgattcctcc ttcgacgaca tgtgcgatcg ctgcgcgacc gaccgctttc ttcagttcgt
     4321 cctgggtcat gattgttctt tgcctcgtcg tcggtggtgt gtcgaagggt ggcattgtag
     4381 cgaacgccgg tgattgaaga cagccgtagc ttcacgacgc ccgtggagga gcgctgtgcg
     4441 acaagccggg ttggcgcggg tggcaggcgc caggggaaat cagcgataat ccgctcgaac
     4501 catcaacagg aggcgcgagt ggctgacgaa agtccgttca aggggcagac cgggatccgg
     4561 cgcatatgga acgccttgca tttctcaggc gccggcctgc gagcggctta tgcggcggaa
     4621 gacgcttttc ggcaggaggt gctgctcgcc gcgctgctca tacccttggc cttcttcctg
     4681 tcgccgaatg gcctggggcg cgcgctgctg gttggcagcg tgttgctggt gttgatcgtc
     4741 gaattactca attcggcgat cgaagccacc gtcgatcgga tctcgatgga gcggcatccg
     4801 ctggccaagc gggccaagga catcggtagt gctgcggtgt tgctggcgct gttcaatgtg
     4861 ctggcgatct gggcctgcgt gttgatcggc tgaacggggt ctctgccggc atcctgtccg
     4921 tcatgcccgt tgggtctgcg cgaagcggtc gcggacccgt cgccccggtg ttcttcaaat
     4981 gatgcggcga agatggatca gcaccgaaat cttcgctccc agccagccca gggcgccgcc
     5041 aatggcggtc aggacaagga tgtcactgac cgatagacct tgcagggcga agttggcacc
     5101 gtagaggcgt gcaagttcgg ctaccggtgg ggtgaggagt tgaaaaccgg cgctcaccaa
     5161 tagggccgca cacaagcccc caagcgcacc ttgtaacagc ccgaaatagt ggaggggccg
     5221 ctgaatgtag gtatcggttg ccccgatcag tttggcaagt tcgatttcgg cagcctgcgc
     5281 gaggatttgc agacgaatgg tgttgaaggt gatggtcacc agagcgccgg caaagaggac
     5341 agcgagcagg atgacgacga gtttgccgag gcggagcagg gcatcgaagc gtttgaccca
     5401 ggccgagtcg agttggacgt gcgcaacctt tggccaggtg gaaaatatcc tggccagact
     5461 ttcgagcgtt tcaggctgcg cctctgtcgg ggtgatgatg tacgcgtccg gcagcgggtt
     5521 tcgcggcagg ctggcgatga tttcggacat gccttcggag gcctgcaggc gcttgagcgc
     5581 ttcctcgcgc gggacgaaac gccaggggcc ggccctgact tccttgagcc gggattcgat
     5641 ttcagaatgc tcgcgtttgc cggcatcgag ggccaggaaa atgctgatct gttgtacgcc
     5701 gggtgtctcg ccggtcagct tgcgcaaatt gtcgagagtc agccagcccg ccgtcggcag
     5761 ggtcagcgcg acgccgatga ccagcagcga cagcagcgaa ttgagcggtg ctgctgccag
     5821 gcgacgcagc gcgtcccgca gcgccgccag atgttgcgcg agccatgcac tcacgcggta
     5881 ttcccctcgg tactctgggc ttgcgcgtcg ctcatctcgc cctggtcgag gcgcagaatt
     5941 cgtggctgca gatgcggcag atgcaggggg tcgtgggtgg cgatcagtac ggtcactcca
     6001 acctgttgaa aagcgcggaa gatctcgagg atctcgcccg ccgatgcggc atcgaggttg
     6061 gcggtgggtt cgtcggcgag aatgatggcc ggtcgattga cgaccgcacg ggcaatcgcc
     6121 aggcgctgtt gctcgccgcc cgagagggcg attggaaagg ccttttcgcg attgggcaga
     6181 ccgaccttgg ccagcgcggc ttgtgctcgc cgcatcgctt cgcgggccgg gtggccaacg
     6241 attgccagcg gcaacagcac gttgtcgagc accgtgcggt cgaacagaag cttgcgatcc
     6301 tggaagatca ggccgagctt tcggcgcagg tacggaatgg cgttcctgcg cagtgaagcg
     6361 aggttctggc cgttgatcac gatgctgcca gacgtcggac gctcgatcgc ggcgagcagt
     6421 ttgaagaggg tcgatttccc ggcgccggag tggcccgtta gaaacaccat ctcgcctgcc
     6481 gtgatttcaa agctcacttt cctgagcgct tcacggcctt ccgggtagcg tttggtgacg
     6541 tcgcaggcgg tgatcactcg aacatggcat ctacaaaatc gcgggccacg aacggccgca
     6601 gatcgtcaat gctctcgccg acgccgatga agcgaacggg tttcgggcac tggcgggcga
     6661 tcgcggcgac caccccgcct ttagccgaac catcgagctt ggtcagcacc aacccggtga
     6721 cctggatggc cttgtcaaag gccatcacct gttgcagcgt gttctggcca aaggtggcgt
     6781 cgagtaccag cagcgtctca tgcgggccgg acggatcggc cttctggatg acgcggcgca
     6841 ccttcgcgat ttcgtccatc aggtgcaact gcgtcggcag gcggccggcg gtgtcggcga
     6901 gcacgatgtc gataccgcgt gctctggcag cggcaatggc gtcgaagatc actgccgccg
     6961 ggtcgccgga caactgtgag acgacgctga cgttgttgcg ctcgccccag gtctgcagtt
     7021 gctcacgtgc cgccgcacgg aaggtgtcgc cagcagccag cagtacggac aaaccctggg
     7081 cctggaagtg tttggccagc ttgccgatcg aggtcgtctt gccggcgccg ttcacgccgg
     7141 cgatcatgat gatgaacggg cggtgcgagg caagatcgag aggttcttcg aggggctgca
     7201 ggagttccag caggatttcc gacagcgctc gctggatggc ctgcggggtg tcgagcttgt
     7261 cgcgtttcgc ccgtaccttc aggtggtaga gaaggtgctg ggtagcgtcc aggccgacgt
     7321 cactgcccag cagcagggct tcgagatctt cgagcaattc gtcgtcgacc ttgccgcgtg
     7381 agaagagcaa cttcagcttg ccgccaaact gcgcccgggt gcgtgcgagt ccggccttga
     7441 gtcgctcgcg ccacggtacg gcggcggcat tgaccgcgac ttcggtatcc gccggtgtgg
     7501 ttggcgtggg cttctggccg ggttgcttct taaggaaacc aaacatggga cttccgtgtc
     7561 ttaggggcgc tcggtaaggt cggcagtggt aagatgccgg cgcaattacc cgacaacctg
     7621 aaattctatc agaagcgact catgcccatg cgtcacgcgc gattgatccc cctgttgctg
     7681 gctctggctg cgccagcgcc ggtgcttgcg gaaacttatg aacaccaatt ggcaaacggc
     7741 ttgcggatca tcgtcaagga agatcggcgc gcacccaccg cggcgcacat ggtctggtat
     7801 cgggctggca gcatggacga gaccaacggc accacgggtg tcgcgcatgt gctcgaacac
     7861 atgatgttca aggggacgcc gagcgcgggc cccggcgagt tcaaccgccg cgtggcagcc
     7921 gccggcgggc gggacaatgc cttcaccggt catgattaca ccgcctactt tcagcaggtt
     7981 ccaaatcaga aactgtccga gatgatgcag cttgaggccg atcgcatgcg ccatctgaca
     8041 cttgatgcgc aggagttcgc gcaggagata cgggtggtca tggaagagcg gcggttgcgc
     8101 acggaggacc agccgcaggc gctgctcttc gagcaactgt cggcagtggc ttttcaggcg
     8161 catccgtatc gtgtgccggt catcggctgg atgaatgatc tggagaacat gaccgtgaat
     8221 gacgcgcgcg cctggtacga gcactggtac gcaccaaaca acgcctacgt cgtggtggtc
     8281 ggtgacgttg accaccagac cgtcttcgcg gcggccgagc aatactacgg acctttgccg
     8341 gcgcgtccgc tgccgggcag gaaacctcag gacgagcccg tgcagaccgg catacgccgc
     8401 ctgacggtga aggcgccggc cgagttaccg gtggtgatga tggcctacaa ggtgccggtg
     8461 ctccgtgacg tcgatcagga cgtcgagcct tatgcactcg aaatgctctc ggcggttctt
     8521 gccggccacg aagcggcgcg ctttaccaac catctgattc gtcagcaacg cttggcggtg
     8581 gatgcgacgg ccagttacga ttccacccgg cgtggtccag gcgtcttcca tctgtacgga
     8641 tcgcccagcg aagggaagac tcgtgccgac ctggagtccg gtctgcgcgc cgaaattgcc
     8701 ttggtgcaag cacagggggt gactccagac gaactggcgc gggccaaggc acagttaatt
     8761 gccgggcaga tctacaagct ggattcgata tttgcacagg ctatggaaat cgggcaactg
     8821 gaatcagtgg gcattcccaa tgcccagaac cggcgcatga tcgaaaagtt gcaggcggtc
     8881 accgccgagc agatccaggc ggtggccaga aagtattttc gcgacgagca gttgaccgtg
     8941 gccgaactcg atccgcagcc cctgccgaca gcgccgcgcg cgcgttcgcc attcgccccc
     9001 cgtcattgag agggtcctca tgaatccgag catgctgttg atcggcctgt gtctggcgct
     9061 tttcgcgcag ttgagtcacg ccggcccgaa gatcgagcgc tggcagacga cttcgggcgc
     9121 gcgtgtccta ttcgtcgaga accatagtct tccgattgtg gatgtccagg tcgatttcgc
     9181 cgcgggtacg gcacaagacg gcgagggcaa ggctggcgtg gcttccctga cccgaggtat
     9241 cctcgatctg ggtgtccccg gcatggacga gacgcagatc gccagcagca tggccgatat
     9301 cggggcgctg ttgtcgggcg gcgtggacat ggatcgggcc tcggtcacgc tgcgaacgct
     9361 gtcgatggct gacaagcgcg gaccggcgct ggagatgctg cgtgccatcc tgagtcagcc
     9421 gcagtttcct gccgcggtct tcgaccgtga gcaagcacgt tccatctctg ccctgaagga
     9481 ggccttgacg cgtcctgacg ccattgccag caaagccttc tgggcggcaa tgtacgtcgc
     9541 gcatccctac ggacggtatg cgacgccgga atcggtgagt tccctcgcac gtgccgatgt
     9601 cgtcgctttt cacgctgccc attacacggc ccaacgcgcg acggtggcca ttgtcggaga
     9661 tctgtcacgt gcccaggcgg agtcggtcgc cgagcaactc accgtcgggc tcccgtcggg
     9721 acccgccatg gccggggtcg tgttgcccaa attgccgtct ggaggcgaac agcggattgc
     9781 gcatccggcg gtgcaggcgc atctccttct cggtatgccg gcactccggc gcggcgatcc
     9841 ggacttcttt cccttgcttg tcgggaacta ttccctcggt ggcggtggtt ttgtttcgcg
     9901 cctgatgaag gaggttcgcg acaagcgtgg cctagcgtat agcgtacata gctatttcct
     9961 gccgttgcag cagttgggcc ccttccagat cggcctgcag accaagaagg cgcaggccaa
    10021 cgatgcactg aaggttaccc gcgaagtact ggccaccttt ctggccgaag gaccgagtga
    10081 aacggaattg caggcggcca agcagaacct gatcggcagc tttccgttac gcctcgacag
    10141 caaccgcaag attctggaga atgtcgcgct gatcggattt tatgacttgc cactcgacta
    10201 tctcgatcgc tacccggaaa acatagaaaa gg
//
LOCUS       DAOSIT010000013        10123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6456_length_10123_cov_10.938518, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000013 DAOSIT010000000
VERSION     DAOSIT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6456_length_10123_cov_10.938518"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..915
                     /locus_tag="PLL14_00760"
     CDS             4..915
                     /locus_tag="PLL14_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOG02474.1"
                     /translation="MASRLSERGVRVTIPTRHRDNTKKLIMLPTVDMVEADVQDPQTL
                     ARLMQGQDAVINLVGILHDSDSRLPYGKGFAAAHVDLPRKIVAAMKASGVSRLVHMSA
                     LKAAINAPSEYLCSKGEGEACIDAAADDLAVTIFRPSVIFGPDDAFLNTFAKLIKLFP
                     VLPLGAGEARFQPVYVGDVARSFVDCLGESTTFGKTYELCGPKVYTLREMVEYTARLV
                     GKSPRIIDLGTGGWAYLQAGLLWLLPNPPLSPDNLRSMEVDSVTDGAHNYPGWHPTAL
                     EAVAPGYLSSNEMPQPRLDRYRYRAGR"
     gene            940..>1478
                     /locus_tag="PLL14_00765"
     CDS             940..>1478
                     /locus_tag="PLL14_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOG02475.1"
                     /translation="MLTLVIGDRNFSSWSLRPWLTARQAGLPFTEVAIRLDRSNTKEE
                     ILQYSPSGKVPCLIDDQGGERLLIWDSLAICEYLAELVPSLWPDERAQRAEARALCAE
                     MHSGFLPLRQEMPMDVRASRPGQPRSPAVCADIRRIVAIWEACRSRFATSGPFLFGAF
                     SIVDAMYAPVVWRFITYGVG"
     assembly_gap    1479..1578
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1579..2658
                     /locus_tag="PLL14_00770"
     CDS             <1579..2658
                     /locus_tag="PLL14_00770"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HOG02476.1"
                     /translation="TERKTGHGYKGFAFHAAPEVSLEEDLARRDLTINAIARAADGSL
                     IDPYRGVDDLAARVLRHVGPAFGEDPVRVLRVARFAARLVDFSVAPETLALMQAMVDG
                     GELDHLVAERVWQELAKGLMEVRPSRMFVILRACGALARLLPELDRLFGVPQRADYHP
                     EVDTGVHVMMVVDQSALRGCALPVRFAALLHDLGKGTTPATDLPRHPGHEARSVDLVG
                     EVCARFKVPVDCRDLALLVARYHGDIHRGPELAAATIVRLLEATDALRRPARFEQLLA
                     ACACDFHGRRGWQDVPYRPPALFRQALALLRGVDAAAIARACDDRAGIAARVHAARVA
                     AVRIGLSNRSDEAFGRAGPALNEGG"
     gene            complement(2673..3851)
                     /locus_tag="PLL14_00775"
     CDS             complement(2673..3851)
                     /locus_tag="PLL14_00775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG02477.1"
                     /translation="MLITRTPGTLPLPTADALACSAALCSRIVAEIDAAGGWIGFDRF
                     MDLALYTPGMGYYAGGAHKFGAAGDFLTAPELSDAFAQALGAQVAQLLALGAPQIIEV
                     GAGSGQLAADLLLELDRRDRLPERYCILELSGELRARQHATLSTRVPQFLPRLCWLEC
                     LPEDFDGLVLANEVLDAMPVHLVVWNGEDIAERGVAVDNGQFTWRDRPAGGRLRERAQ
                     ALAAACAIAPGYLSEISLLAPAWIAQWAAILRRGALLLIDYGFPRHEYYHPQRDAGTL
                     MCHYRQHAHAEPFFLPGLQDMTAHVDFTAIVEAGCEAGLELLGYSTQASFLFNCGLTE
                     ILARTPASEPLRYLPLASAVQKLISPAEMGELFKVIALGKGISEPLLGFANGDRSATL
                     "
     gene            complement(3869..4723)
                     /locus_tag="PLL14_00780"
     CDS             complement(3869..4723)
                     /locus_tag="PLL14_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02478.1"
                     /translation="MSNLDRPLDADELITLAERIAQLPAADAEWVGMLLRELLRARAR
                     EAELLAEQASVRHETEAHSGELDDQLAQVALDTAEWLRTLWNVGYMGAGSFRSQPRSA
                     FPAIDLEDVRKSSLFARIRQGKHALPFPPPTRQGLPWHELLEDSGQRSHRVDAEVVRE
                     ETGLAFGAIIEGCAEWQVMEECVENKEFVVQHQGKGPTYRLRLGDGATARLRREPPHT
                     TRKIHLQGHGGFHSYTLEWPADGDGRTQYVPLRAATWTRAESEAEHWLASTHPELYGQ
                     VRFEICEL"
     gene            4858..>5042
                     /locus_tag="PLL14_00785"
     CDS             4858..>5042
                     /locus_tag="PLL14_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG02479.1"
                     /translation="MNRKTILVTGAARRVGSAIARALHATGANILVHYHLASEAAAAL
                     VAELNLARPSSAACLQA"
     assembly_gap    5043..5142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5143..5430
                     /locus_tag="PLL14_00790"
     CDS             <5143..5430
                     /locus_tag="PLL14_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG02480.1"
                     /translation="PLYCAAKAGLLGLTRALAIELAPLVRVNAVAPGPILWPESDLFD
                     SAARAEILAHTLLQRAGCPQDIAHAVRYLLEDAAYVTGQVINVDGGRTAHL"
     gene            5457..6344
                     /gene="ttcA"
                     /locus_tag="PLL14_00795"
     CDS             5457..6344
                     /gene="ttcA"
                     /locus_tag="PLL14_00795"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOG02481.1"
                     /translation="MELSNTLLRLKKRLEGKVGKAIADYHMIAHGDTVLVCLSGGKDS
                     YTLLSILMALRERAPVNFRLIAMNLDQKQPGFPADVLPNYLAAIGVEYRIVEQDTYSV
                     VKSKIPEGKTTCSLCSRLRRGIIYRTAKEVGANKIALGHHRDDIVHTLFLNLLFGGQL
                     KAMPPKLVTDDGAHVVIRPLAYCSEADIARFARAMEYPIIPCNLCGSQDNLQRQKIRE
                     MMGDWDRRYPGRTLSVFSALQKVIPSHLADHALFDFRGLRPGADLTTMAGGDTVFDPQ
                     PHDTVATAPAAFPLPVVSA"
     gene            6414..>7141
                     /locus_tag="PLL14_00800"
     CDS             6414..>7141
                     /locus_tag="PLL14_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOG02482.1"
                     /translation="MSSQERTLSVVVAVVDETPRLFEKLGGAEALHAIERCMKRMMRG
                     IDGFHGHVVRSRRNDLTAIFANANDACQATIAMQRRIADLPPVSGMQLAIRIGFQHGT
                     VFEQGGEFLGDCVNTAECLAELAVAGQVLTGGEVRALLSPHLQSSTRHLQHLDTHELP
                     TKTQVFEVPWLEPRAAAAPVVIPPRPAPERDLRLCVRYGGDVKLLDKQRPTLVMGRDA
                     ACDINVRDRRASRHHARIERRGEH"
     assembly_gap    7142..7241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7242..7462
                     /locus_tag="PLL14_00805"
     CDS             <7242..7462
                     /locus_tag="PLL14_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOG02483.1"
                     /translation="DRRASRHHARIERRGEHFVLRDLSTNGTFITVSGEHELFLRHEE
                     FVLRGSGIISFASSANHPSADIAEFEHL"
     gene            complement(7521..8201)
                     /locus_tag="PLL14_00810"
     CDS             complement(7521..8201)
                     /locus_tag="PLL14_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG02484.1"
                     /translation="MVRRSCARLLAQDCLLCSAESGNNLLCPACENDLPRLATARCPR
                     CALPTPGGEVCGRCLARLPHYDATLAVFRYGFPIDKLVQSFKYGHRLALGSYFGRQLA
                     AQTGRFAADLLIPMPLHPQRLRERGFNQALELARPVGKAWRMPVDARSCCRIRHTAAQ
                     ADLPWRERAGNVRGAFACAADFTGRRVILIDDVMTTGASLNELARTVKLHGAAEVTLL
                     VLARALPP"
     gene            8425..9414
                     /gene="bioB"
                     /locus_tag="PLL14_00815"
     CDS             8425..9414
                     /gene="bioB"
                     /locus_tag="PLL14_00815"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOG02485.1"
                     /translation="MNAIPQAAALTPDTAAAGQAARWTVAEVEKLYQLPLMDLIFRAQ
                     QVHREHFDANEIQRSTLLSVKTGGCSEDCGYCSQSARYSTDTEREALLPLDEVVAAAQ
                     AAKDKGASRFCMGAAWRGPKDKDLAKVSEMIRAVKGLGLQTCVTLGMLKEGQASELKE
                     AGLDYYNHNLDTDASFYGQVITTHQHADRVDTLGQVREAGIKVCSGGIIGMGEGRRNR
                     AALIAQLANLNPPPESVPINNLVPIPGTPLANVAPIDNFEFVRTIAAARITMPTSFVR
                     LSAGRQAMPEEVQALCFLAGANSIFYGDKLLTTGNPEADRDEALFEKLGLRPI"
     gene            9534..>10123
                     /locus_tag="PLL14_00820"
     CDS             9534..>10123
                     /locus_tag="PLL14_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG02486.1"
                     /translation="MAGTLYWSSMKTAAQFLDQRQVRRNFARAASTYDQVAVLQREVG
                     RRMLERLDYVRLEPQRVLDLGCGTGASLTALHERYPKAAVIGVDLSEAMLRIGQKQRS
                     RLRWLLPFLPGRRPALLAADAAALPFKPQSIGLLWSNLLLHWLDDPLQGFREAYRLLE
                     IDGLLMFSTFGPDTLKELRASFGDGYPHTQRFTDMH"
BASE COUNT         1741 a         3190 c         3127 g         1765 t
ORIGIN      
        1 tggattgcca gccgcctttc ggaacgcggt gtccgggtga cgataccgac ccgccatcgc
       61 gacaatacca agaagctcat catgctgccg acggtggaca tggtcgaggc cgacgttcag
      121 gatccgcaga cgctcgccag gctgatgcag gggcaggacg cggtgatcaa cctggtcggc
      181 atactgcacg acagcgattc gcggctgcct tacggcaagg gtttcgctgc agcgcatgtc
      241 gatctgccgc gcaagatcgt cgccgcgatg aaggcaagcg gcgtcagccg cctggtgcac
      301 atgagcgcgc tcaaggcggc gatcaacgcg ccttccgagt acctctgttc gaaaggcgag
      361 ggcgaagcct gtatcgacgc ggctgccgat gacctggcgg tcacgatctt ccgtccttcg
      421 gtgatctttg gtccggatga cgcctttctc aacaccttcg ccaagctgat caaactcttc
      481 cccgtcctgc cgctgggtgc tggcgaggcg cgcttccagc cggtctatgt cggcgacgtc
      541 gccaggtcct tcgtcgattg cctgggcgag agtaccactt ttggaaagac ttacgagctc
      601 tgtggaccca aggtctacac cctgcgcgaa atggtcgagt acaccgctcg cctggtcggc
      661 aaatcgccac ggatcatcga cctcggcacc ggcggctggg cctacctgca ggctggcctg
      721 ctgtggctgt tgccgaatcc gccgctgtcc cccgacaatc tgcgttcgat ggaagtcgac
      781 agcgtcaccg acggtgcgca caactatccc ggctggcatc cgacggcact cgaggcggtg
      841 gcgccaggtt atctttcgtc gaacgaaatg ccgcagcctc gccttgaccg ttatcgctac
      901 cgggccggtc gctgagcgaa tgaccgggag agcatactca tgctgacgct cgtcatcggc
      961 gatcgcaact tttcttcgtg gtccctgcgg ccctggctga ccgccaggca ggcgggactg
     1021 ccgttcaccg aggtcgcgat tcgcctggac cgcagcaaca ccaaggaaga aattctccag
     1081 tattcgccat cgggcaaagt gccttgtctg atcgacgatc aaggaggcga acgcctgctg
     1141 atctgggact cgctggcgat ctgcgaatac ctcgccgaac tcgtgccctc gctgtggccc
     1201 gacgagcggg cgcagcgggc cgaggcacgc gcgctctgcg ccgaaatgca ttcgggattc
     1261 ctgccactgc gccaggaaat gccgatggat gttcgcgcca gcaggccggg ccagccgcga
     1321 agcccggccg tctgtgccga catccggcgc atcgtggcca tctgggaggc ttgtcgcagt
     1381 cgctttgcga cgtccggacc ctttctgttc ggcgccttct cgattgtcga tgccatgtat
     1441 gccccggtgg tgtggcgttt tatcacctac ggtgtcggnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnac cgagcgcaag accggtcatg gctacaaggg ctttgccttt
     1621 catgccgcgc cggaagtcag cctcgaagag gatctggcgc gccgcgacct gacgatcaat
     1681 gccatcgccc gtgccgccga tggatcgctg atcgatccgt atcgcggtgt cgacgatctg
     1741 gcagcgcgcg tcctgcggca tgttggtccg gccttcggcg aagacccggt gcgggtgctt
     1801 cgtgtcgccc gcttcgccgc gcgtctggtc gatttcagcg tcgcaccgga aaccctggca
     1861 ctgatgcagg cgatggtcga cggcggcgaa ctcgaccatc tggtggccga acgcgtctgg
     1921 caggaactgg ccaaggggct gatggaagtc cgcccttcac ggatgttcgt catcctgcgc
     1981 gcatgcgggg cactggcccg cctgctcccc gagctcgacc gccttttcgg cgttccacag
     2041 cgcgccgact accacccgga ggtggacacc ggtgtgcatg tgatgatggt cgtcgaccag
     2101 tcggcgctgc gcggctgcgc gctgccggtt cgcttcgcgg cgctgctcca cgatctcggc
     2161 aaaggcacga cgcctgctac cgatctgcca cggcatccgg gacatgaggc acgcagtgtc
     2221 gacctggtcg gcgaagtctg cgcacgcttc aaggtcccgg tggactgccg cgatctggcc
     2281 ctgctggtgg cacgctacca tggcgacatc catcgcggac cggaactggc ggcggcaacc
     2341 attgtcaggc ttctcgaagc tactgacgcc ttgcgccgac ccgcccgctt cgaacaactc
     2401 ctcgcggctt gtgcctgcga ctttcatggc cgtcgcggct ggcaggacgt gccctatcga
     2461 ccaccggcgc ttttccgtca ggccctggcg ctgctgcgcg gagtcgacgc cgcagcgatt
     2521 gcgcgggcct gcgacgatcg agccggcatt gctgcgcggg tgcacgcggc gcgcgtcgcg
     2581 gcggtgcgga tcgggctgtc aaatcgcagc gatgaggctt tcgggcgggc tggtccggca
     2641 ctgaacgagg gaggatgagc ggtgggccag gctcacaagg tcgcgctgcg gtcgccgttc
     2701 gcgaaaccca acaggggttc gctgatgcct ttgcccaggg cgatgacctt gaagagttcg
     2761 cccatttctg caggtgagat cagcttctga acggcgctcg ccagcggcag gtagcgcagc
     2821 ggttcgctcg ccggcgtgcg ggccaggatt tcggtcagcc cgcagttgaa aagaaagctc
     2881 gcctgcgtac tgtagcccaa caactcaagt ccggcctcgc agccggcttc gacgatggcc
     2941 gtgaaatcga catgcgcggt catgtcctgc aggcccggca ggaagaaggg ctcggcgtgc
     3001 gcgtgctggc gatagtggca catcaacgtc ccggcgtcgc gctgcggatg gtaatactcg
     3061 tgccgcggga aaccgtagtc gatcaacaac agcgcaccgc gtcgcaggat cgctgcccat
     3121 tgtgcaatcc aggccggagc aagcagggaa atttcgctga gataccccgg cgcgatcgcg
     3181 catgcggcgg ccagcgcctg tgcgcgttca cgcaagcgcc cgccggccgg gcgatcgcgc
     3241 caggtaaact gcccgttgtc cacggccacc ccccgctcgg caatgtcttc cccgttccac
     3301 accacaaggt gcaccggcat cgcatcgagg acttcgttgg ccaataccag accgtcgaaa
     3361 tcctcgggca ggcactcaag ccagcagagg cgcgggagga actgcggaac gcgcgtgctc
     3421 agcgttgcat gctggcgcgc gcgcaattca ccggacagtt cgagaatgca gtagcgttca
     3481 ggcagccggt cgcgccggtc gagttcgagc aacaggtcgg cggcgagttg tccgctgccg
     3541 gcgccgacct cgatgatctg cggcgcgccg agcgcgagta actgcgcgac ctgagcgccg
     3601 agcgcctggg cgaaggcatc ggagagttcc ggcgcggtca ggaaatcgcc ggcagcgccg
     3661 aacttgtgtg ccccacccgc gtaatagccc atgccgggcg tgtagagagc gaggtccatg
     3721 aagcgatcga aaccgatcca gccgccggcc gcgtcgattt cggcgacgat tcgggaacac
     3781 aacgcggcgc tgcaggccag ggcatccgcc gtcggaagtg gcagagtgcc tggggttcgg
     3841 gtgatcagca tgccgatcag gtcatgcttc agagttcaca gatttcgaag cggacctgtc
     3901 cgtacaattc cgggtgcgta ctcgccagcc agtgctcggc ctcggattcg gcgcgcgtcc
     3961 aggtcgcggc gcgcaaaggc acgtactggg tacgcccgtc gccatcggcc ggccattcga
     4021 gggtgtagct gtggaaaccg ccgtgcccct gcaggtggat cttgcgcgtc gtatgcggtg
     4081 gctcgcggcg caaccgagcg gtcgcgccat cgcccagacg cagccgatag gtcggcccct
     4141 tgccctggtg ctgcacgacg aactccttgt tctcgacgca ctcttccatc acctgccact
     4201 cggcgcagcc ttcgatgatc gcaccgaagg ccaggcccgt ttcctcgcga acgacctcgg
     4261 cgtcgacgcg atgcgagcgc tgccccgagt cctccagcag ttcgtgccag ggcaagccct
     4321 ggcgggtcgg cggcgggaat ggcaaggcat gtttgccctg gcgaatgcgc gcgaaaagcg
     4381 aggacttcct gacatcttcg aggtcgatcg ccggaaaggc cgaacgcggt tgcgaacgga
     4441 aactgccggc ccccatgtag ccgacgttcc acagcgttcg caaccactcg gcggtgtcga
     4501 gagcaacctg cgccagttgg tcgtcgagtt caccgctgtg cgcctcggtt tcgtgccgga
     4561 cgctggcttg ttcggccagc aactccgctt cgcgcgcgcg cgcgcgcagc aactcccgca
     4621 gcagcatgcc aacccactcg gcatcggctg ccggcagttg ggcgattcgc tcggcaagcg
     4681 tgatcagttc gtcggcgtcg agtggtctgt ccaggttgct catcaacgat ctcccattct
     4741 tacacgtgaa ttcctcgcag tttagcgcgc tggcgcccga aaggactgct ttttccgaag
     4801 gttggggcag gaatccgtta aaatcaaatt cttgttggtg caggcctgga tctggagatg
     4861 aaccgcaaaa cgatattggt aaccggcgct gccagacggg tgggcagtgc gatcgcgcgc
     4921 gcgctgcacg ccaccggagc caacatcctg gtgcactacc atctggcgtc ggaagcggct
     4981 gcggctctgg ttgccgaact gaatctggca agaccgtctt cggcggcctg cctgcaggcc
     5041 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccctgta ttgcgccgcc
     5161 aaggcgggcc tgctcggact gacgcgggcc ctggccatcg aactggcgcc gctggtacgg
     5221 gtcaacgccg tggcgccggg tccgatcctg tggccggaga gtgatctctt tgacagcgcc
     5281 gcgcgcgccg agatcctggc gcataccttg ttgcaacgag ccggttgtcc gcaggacatc
     5341 gcgcacgcgg tacgctacct gctcgaggac gcggcctacg ttaccggcca ggtcatcaac
     5401 gtcgatggcg gccgtaccgc gcatctgtag tccgtcacgc catcagagag gactccttgg
     5461 aactgtcaaa caccttgctg cgcctcaaaa agcggcttga aggcaaggtc ggcaaggcga
     5521 tcgccgacta ccacatgatc gcgcacggcg acactgtcct ggtttgcctt tccggcggca
     5581 aggactcgta cacactgctg tcgatactga tggcattgcg cgagcgtgcg ccggtgaatt
     5641 tccgtctgat cgccatgaac cttgaccaga agcagccggg cttcccggct gacgtgctgc
     5701 cgaattatct ggcggccatc ggggtcgagt accggatcgt cgagcaggac acctacagtg
     5761 tcgtcaagag caagattccg gagggcaaga ccacctgctc gctgtgctcc cgtctgcggc
     5821 gcggcatcat ttatcggacc gccaaggaag tcggcgccaa caagatcgcc ctcggccatc
     5881 atcgcgatga catcgtccat acgttgtttc tcaatctgct cttcggcggc caactcaagg
     5941 cgatgccgcc caaactggtc accgacgatg gcgcacacgt ggtgatccga cccctggcct
     6001 actgcagcga agcggacatc gcgcgtttcg cgcgcgccat ggaatacccg atcatccctt
     6061 gcaatctctg cggttcgcag gacaatctgc agcgccagaa gattcgcgaa atgatgggag
     6121 actgggatcg acgctatccc ggtcgcacgc tatcggtgtt ttctgcactc cagaaggtga
     6181 ttccctcaca tctggccgac catgccctgt tcgacttcag gggcctgcgc ccgggcgcgg
     6241 atctgacgac gatggctggc ggcgataccg tgttcgaccc gcagccgcat gacacggttg
     6301 cgactgcacc ggcagcattc cctttgccgg tggtaagcgc atgacacgat cgagcatgcc
     6361 tgccgcgcgc agtgctggcc acacgctaac cggcagccgg gagagtggca ttcatgagta
     6421 gccaggaaag gacgctgtcg gtcgtcgtcg ccgtcgttga tgaaactccg cgtctgttcg
     6481 agaagcttgg cggcgccgag gcgctgcatg ccattgagcg ttgcatgaag cgcatgatgc
     6541 gcggcattga cggtttccac ggtcacgttg tacggagccg ccggaatgac ctcacggcaa
     6601 tcttcgcgaa cgccaacgat gcctgccagg caaccatcgc catgcagcgg cgcatcgccg
     6661 acctgccgcc tgtttcaggc atgcaactcg ccatccggat cggttttcag cacggcacgg
     6721 tgttcgagca ggggggcgag ttccttggcg actgcgtcaa taccgcagag tgcctggccg
     6781 aactggcggt cgccggacag gtgctgaccg gcggtgaagt gcgggccttg ttgtcgccgc
     6841 atctgcaatc gtcgacccgc cacctgcagc acctggacac ccatgagctg ccgacgaaaa
     6901 cacaggtttt cgaagtgccc tggctggaac cgagagctgc ggcagcgccg gtggtgatac
     6961 cgccaaggcc ggccccggag cgcgacctgc gactttgcgt acgttatggc ggggacgtga
     7021 aactgctcga caagcaacga ccgacactgg tcatgggacg tgatgcggcg tgcgatatca
     7081 acgttcgtga tcgccgcgcg tcgcgtcacc acgcacgcat cgaacggcga ggcgagcact
     7141 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgatcgcc gcgcgtcgcg
     7261 tcaccacgca cgcatcgaac ggcgaggcga gcactttgtg ctgcgcgatc tcagtaccaa
     7321 cgggactttc attaccgtca gcggcgagca cgaactgttc ctgagacatg aggagttcgt
     7381 gctgcgcggc agcggcatca tctccttcgc ttcatcggca aaccacccga gcgcggacat
     7441 cgccgagttc gaacatcttt gagtcgtggc gccgccgaac cgctgctgca tggccggcat
     7501 ttgccgatcg ttccgggaga ttacggcggc aaggcgcggg caagcaccag cagcgtcacc
     7561 tcggctgcac catgcaattt gacggtgcgt gccaattcgt tgagggacgc gcccgtggtc
     7621 atcacgtcgt caatcaggat cacccgcctg ccggtgaaat ccgccgcgca agcgaaggcg
     7681 ccgcgaacgt tcccggccct ttcgcgccag ggaaggtcgg cctgggcagc ggtatggcgg
     7741 attcggcaac aactgcgtgc gtcgacgggc atgcgccagg ccttgccgac cggacgagcc
     7801 agttcgagcg cctggttgaa gccacgctcg cgcaggcgct ggggatgcag cggcatgggg
     7861 atgagcaggt ccgccgcgaa gcgcccagtc tgcgcggcga gctgccgacc aaagtagctg
     7921 ccgagcgcca ggcgatgtcc atacttgaac gactgaacca gcttgtcgat cggaaaacca
     7981 taacgaaaaa cggccagggt ggcgtcgtaa tgcggcaacc tcgcgagaca acggccgcag
     8041 acttcgccac cgggcgtcgg cagcgcgcag cgtggacagc gtgccgtcgc gagtcgcggc
     8101 aagtcgttct cgcaggccgg gcagagcaga ttattgccac tttcggcgct gcacagcagg
     8161 cagtcctgag ccagcagtcg tgcgcacgat ctgcggacca aggcggcgct ttgagttaag
     8221 attgacaccc gtgcagtgct cgtaaagaat ccgtgatgac ggaccttaca ttattccagc
     8281 agacgtgact ttgccagccc ccgcgagcat gcaggtcata cccgtcccgc tcaatccgac
     8341 tcttctcccg catccggaag aagggagaat ctttttcgtg agtcgccgaa gcgacgttca
     8401 cggtaactct caggtctaca actcatgaac gcaatccctc aagccgccgc gctcaccccc
     8461 gataccgccg cagcgggcca agccgcccgc tggaccgtcg ccgaagtcga aaagctctat
     8521 cagctaccgc tgatggacct gatttttcgt gcgcagcagg tgcatcgcga gcacttcgac
     8581 gccaacgaga ttcaacgctc gacgctgctc tcggtcaaga ccggcggctg ttccgaggac
     8641 tgcggctatt gctcgcagtc cgcccgctac agcaccgaca ccgaacgcga agccttgctg
     8701 ccgctcgatg aagtggtcgc cgcggcgcag gccgccaagg acaagggcgc ctcgcggttc
     8761 tgcatgggtg cagcctggcg tggtcccaag gacaaggacc tggctaaagt gagcgagatg
     8821 attcgcgccg tcaagggcct gggtctgcag acctgcgtca ccctgggcat gctcaaggaa
     8881 ggccaggcca gcgaactcaa ggaagcggga ctcgactact acaaccacaa cctcgatacc
     8941 gatgcctcct tctacgggca ggtgatcacc acccaccagc acgccgaccg cgtcgatacg
     9001 ctggggcagg tgcgcgaggc cggaatcaag gtctgttccg gtggcatcat cggcatgggc
     9061 gagggacgcc ggaaccgcgc ggcgctgatc gcgcagctcg ccaacctcaa cccgccgccg
     9121 gaatcggtac ccatcaacaa tctggtcccc attcccggta cgccgctggc caacgtcgcg
     9181 ccaatcgaca atttcgagtt tgtgcgcacc attgccgccg cacgcatcac catgccgacg
     9241 agcttcgtac ggctctccgc ggggcgccag gcgatgcccg aagaagtgca ggcgctgtgt
     9301 tttctggctg gcgccaactc gatcttctac ggcgacaaac tgttgaccac cggtaacccg
     9361 gaagccgacc gtgatgaagc gctgttcgag aagctcggtc tgcggccgat ctagtcgctg
     9421 gttgccgtgc aatgcccagc ccagagccac gctgttcggc ctgagcgcgt cgcgggccgg
     9481 caggcaaacc gtcaccccgg cgatccatcg ctttcacctc gagtggtaag ggtttggctg
     9541 gaacactcta ctggtcgtcg atgaagactg ccgcgcagtt cctcgaccag cgccaggtgc
     9601 gacggaattt tgcgcgcgcg gcctcgacct acgatcaggt cgcggttctg cagcgcgaag
     9661 tcggccggcg catgcttgaa cggctggatt acgtgcgcct cgaaccgcag cgtgttctcg
     9721 atctcggctg cggcaccggc gccagtctca ccgctctgca cgaacgctat ccgaaggctg
     9781 cggtgatcgg cgtcgacctc agcgaggcca tgctgcgcat cgggcagaaa cagcgctcgc
     9841 gcttgcgctg gttgttgccg tttctgcccg gcaggcggcc ggcgctgctg gcggcggacg
     9901 cggcagcctt gcccttcaag ccgcagtcga tcggtctgct gtggtcgaac ctgttgctgc
     9961 actggctcga cgatccgctg cagggtttcc gcgaggcgta tcgactgctc gaaatcgacg
    10021 ggctgctgat gttctcgacc ttcggcccgg acaccctgaa ggaactgcgc gccagttttg
    10081 gcgacggcta cccgcatacc cagcgcttta ccgacatgca cga
//
LOCUS       DAOSIT010000014        10068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6504_length_10068_cov_7.683412, whole genome shotgun sequence.
ACCESSION   DAOSIT010000014 DAOSIT010000000
VERSION     DAOSIT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_10068_cov_7.683412"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..870)
                     /locus_tag="PLL14_00825"
     CDS             complement(85..870)
                     /locus_tag="PLL14_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02487.1"
                     /translation="MAYEHQQAPITFGYRCGRSGAHASRTMMLAELSTLLAHVPPGAA
                     RWDYQREVVDSNTLGKPTMKARQLTFRHLVELYGLDPSLAVFRVFRQLWNLDEQARPV
                     LALMVALVRDPLLRLSSDFMLAKHPGESVQRAELEALLATDDPDRFTPASRKSFAQNI
                     NGTWTQAGFLVGRVHKTRVVPCITPTNVTFALFLGHLEGLTGERLFASQWMNLLPGSP
                     DDLVMQANSAANRGQIVFMNAGGVKEVRFPGYLSPAEEMWLHE"
     gene            complement(886..>1245)
                     /locus_tag="PLL14_00830"
     CDS             complement(886..>1245)
                     /locus_tag="PLL14_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOG02488.1"
                     /translation="PAESRAQDVDEGASRADSGASIAVVPLTIPLLTGPASISTVVIY
                     AEKARHFWELGVLVGYGLVIGVATWLALSAAGRIGRFMGQTGINVMTRLMGLILAAIA
                     VEVMADGLIKLFPVLGR"
     assembly_gap    1246..1345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1346..2290
                     /locus_tag="PLL14_00835"
     CDS             <1346..2290
                     /locus_tag="PLL14_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG02489.1"
                     /translation="MQSLWMLLASLLFACMGVCVKLVAATHSAVEIVFFRSVLSLLLM
                     YALVRLRGVPLKTVHWRWQVSRGAIGFSALCAYFWAITLLPLATAVTLNYTSAIFLAA
                     YLALSGMRLNVGIIAALALGLGGVGLLLKPTLHAELWIGGLIGLASGVLAGMAYFSVR
                     ELGSRGEPETRTVFYFSLVSSVCASVWLLFCEIHPLDLRSGALLFGVAAFATAAQLAM
                     THAYAGSRTLLAAALAYSTVVFSSVFGILLWNEAHDGWTWLAMALIVISGLAATYFSP
                     GSPATRSSAPPDQPAIESSPRPAGKTQASGKSRASRTS"
     gene            complement(2251..>2572)
                     /locus_tag="PLL14_00840"
     CDS             complement(2251..>2572)
                     /locus_tag="PLL14_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HOG02490.1"
                     /translation="ARWGGEPAATLLACDLQPSVLTLYADKLSARLVAQQKLTAAGPV
                     AYQNLLEVRKPFWGRSLSIIGQRDTVPPALVYADLLATGNPRCLEAARAIYDVRLARL
                     FPEA"
     assembly_gap    2573..2672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2673..2862)
                     /locus_tag="PLL14_00845"
     CDS             complement(<2673..2862)
                     /locus_tag="PLL14_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02491.1"
                     /translation="MNAQKASTLLEKAAQALRGHGLMVSVQKQDGSEGRWRSATCLGV
                     RKDRCGTDYAAEVRCKVTS"
     gene            complement(2939..6442)
                     /locus_tag="PLL14_00850"
     CDS             complement(2939..6442)
                     /locus_tag="PLL14_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG02492.1"
                     /translation="MSQQRPDDCPPSRAVSLRAAKPVFFDPASKRWPRLRLGMTLIGL
                     TLSLLLGALVLSILVSPVLPALNLSGVSFLPQGAHALPAVPALAPERPLTRRERALRN
                     VQNRLASERARELKQLLQSRKAGPVARRQALAIGFFVNWDDSSMSSLKENLGSLDMVI
                     AEWLHLAAEDGSLRENDPTRTAQVTEYVRARRPEVSIVPLVNNWNGHDWEGAKLARML
                     ARPAARARTIEQLVAYVERNRFAGISIDFENVSTKAQPDFQRFMAELYAAMHPKNLLV
                     SVNVPASDPAFDYRRLVRSADYLILMAYDEHWATSAPGPIASLPWFASVLRQRQRDIP
                     AEKMIVAIGNYAYDWQPGQPAEERTFEEAVLVAKESEGKIRLDPASLNPTFAYADDND
                     RIHHVWMLDAVTAFNQLLAIRTLQPRGVALWRLGSEDPALWQIFGKDTPLDAVRAAEL
                     GNIRFAYGVDYEGKGEILEVTAKPQTGSRIIEFDPKRGLISSERFTAFPSPYVITRHG
                     GAERKVVLTFDDGPDLQYTPQVLDALRLAGVPAAFFIMGVNGQMHPELLRRMLDEGHE
                     IGNHTFTHPNISTISPMQFQLELSATQHLLASVVGRHSLLFRPPYAVDAEPETIDQVR
                     PIELAAEQGYLVVGMQIDPDDWKRPGVAEIVRRTVEQAERGEGNIVLLHDSGGDRSQT
                     VQAIPRIVEELRRRGFQFVSVSELLGRSRDALMPPVPPETRWRIWLDGAAFAVLNWSA
                     ATVHWLFLLGIVLGIARLLFVGTLAIYQRWQQRREVFDPEYSPTVTLVIPAYNEEKVI
                     VQTIASLLACETPRKLEIIVVDDGSSDATYRVAREAFAGDPRVRVYSVVNGGKPAALN
                     FGMAQSNAEIVVALDADTVFTRDTIVKLVRHFVDPKIGAVAGNTKVGNRINLLTRWQA
                     LEYITSQNLDRRAFDVLNCITVVPGAVGAWRRELIERVGGFTDLTLAEDADLTLAIRK
                     LGYAIVYEDEAVALTEAPDTVRGFIRQRYRWMYGTMQAAWKHRDVLFRPRYGALGFVA
                     LPNVIVFQVLFPLVSPIMDLLLIGSLGSAAFNHWQHPEEYSPDALWRVLFYYALFVTV
                     DYLAAILAFTLERKESWWLLIWLFWQRFFYRQLMYYVAIKSTLTSLRGVLVGWGRIDR
                     KATVQAVSGSRIREAGAAGVD"
     gene            complement(6423..8675)
                     /locus_tag="PLL14_00855"
     CDS             complement(6423..8675)
                     /locus_tag="PLL14_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOG02493.1"
                     /translation="MILVMTLASPLSSFGAEAPARVTIDLTSGPSNTFVPAVALGAGV
                     DGHSRGDAAAIYRPRNLRAMRSVGLRPLSYRLRTELGVEAWHWNPRGRWSDSEHARGY
                     WTSDDQPGAPILASFGYRLPRRGYTVDQAEDNGYSRLADGDLDTFWKSNPYLDRGFTG
                     EDNSRHPQWLIVDLGKKMPVDAMRIAWGMPYAVRYQVQYWDGEDAALPDEYPSGHWQT
                     FPEGEVRQGSGGDENLRLARHAVAVRYLRVLLEVSSGSPAPGARDARDALGYAVREVY
                     VGALDANDRLHDAVRHAASHDGQTRMFVSSTDPWHRAKDLDPEVEQAGIDRVFTSGLG
                     HGLPMLVPVGVLYDTPDNAAAFLRYLRRRAYPIRGVELGEEPDGQYVAPEDYGALYLQ
                     VTDALRAVDPDVNLGGPSFQSLWDAPMMAWSGTSVAPDRPWLARLLDYLRVRERAADL
                     GFLSFEWYPFDEVCTPPAEQLRQAPGLLTEAMAELYQQGLPRGTPLLMTEYGYSAFST
                     QTAVDLPGALFNADVVGTFLTEGGAVAYLYGYEPGPLDKGPDCDTWGTNTLFLSDQRR
                     KILARTATYHGARLLARQWAGSPTQAHAVYRTRVDDDAAGGVSPVSAYALRRPDGLWS
                     VLLVNKDPQRQRTVALHFSGPEANALTPLQGPADLYQFSAAQYRWRANGAFGRPQRSL
                     PPQYRLLPGHQAPLHLRLPPWSLTVVRSRAPQAGQPNPSAHLGRVYANPQRRTLAVSR
                     PGTHHVAAAS"
     gene            complement(8782..9120)
                     /locus_tag="PLL14_00860"
     CDS             complement(8782..9120)
                     /locus_tag="PLL14_00860"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HOG02494.1"
                     /translation="MDLDLFNSELCLADDAPIRLLSARGVRIRCTSGVVWLTIAGEAG
                     DIFLAAGDSYLVRGRGLALLEAIGGGRVRFEKASLPLSAFFITMLKGCWRRICYFPAT
                     GGEAHVTPSA"
     gene            9269..>10068
                     /locus_tag="PLL14_00865"
     CDS             9269..>10068
                     /locus_tag="PLL14_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG02495.1"
                     /translation="MENELLRYERLAEDLGGIIVAGNLRPGERLPSVRRLSRERRLSV
                     STVLQALRRLEDRGLVEARPQSGYFVRHTAARRSQPNARSTPEAPVPVDVSQRLMRVL
                     QTSMQPGVTPLAAALPACSLLPLAALQRLYGGVARRHPHLLEGGSHVNMDTPALVRQL
                     VLRSVGWAGPLAASEFVITNSCTEALGLCLRAVTQPGDTVAVESPAYYLMLQLLETLG
                     LKALEIPTDPHSGLSIEALDLATRQGKVAACLLVPNASNPLGSIMPDE"
BASE COUNT         1810 a         3194 c         3099 g         1765 t
ORIGIN      
        1 aagatcactt tctgtatcga ggccagcccg gcttgccacg gcaccgtcaa atgctcgcgg
       61 tacgcgctga ccagcttcgc aactttactc atgcagccac atctcctctg cgggcgagag
      121 gtaaccgggg aagcgcactt ccttgacacc gcccgcgttc atgaaaacga tctgtccacg
      181 gtttgctgca gagtttgcct gcattaccag atcatcaggc gaacctggca gcaaattcat
      241 ccattgcgag gcgaacaggc gctcgcctgt cagcccttcg aggtggccga ggaacaacgc
      301 gaaagtgaca ttggtcgggg tgatgcaagg gacgacacgg gtcttgtgca cccgaccaac
      361 cagaaatccg gcctgagtcc aggtgccgtt gatgttctgg gcaaacgatt tccgcgaggc
      421 gggcgtgaag cgatcaggat cgtctgtggc gagcaaggct tccaactcag cgcgttgtac
      481 ggattcgcca ggatgcttgg caagcatgaa atcactggat agacgcagaa gggggtcgcg
      541 caccagcgca accatcaagg cgagcacagg acgagcctgc tcgtcaagat tccacagttg
      601 gcggaagacg cggaacactg ccagactcgg gtcgaggcca tacagttcca ccagatggcg
      661 aaaggtcagt tggcgtgcct tcatggtcgg ttttccaagc gtgttggagt cgaccacttc
      721 gcgctgatag tcccaacgcg ccgcgcctgg cgggacatgc gcgagcagcg tggaaagctc
      781 agcgagcatc atggtccgtg acgcatgcgc cccgctacga ccgcagcggt agccgaatgt
      841 gatgggtgct tgttggtgct cgtaggccat gcatctcttc ggcggtcagc gcccaagtac
      901 cggaaacagt ttgatcagcc cgtcggccat cacttcgacg gcgatggccg ccaggatcag
      961 gcccatcagt cgggtcatga cgttgatccc ggtctgaccc atgaacctgc cgatgcgacc
     1021 ggccgccgac agcgccagcc aggtcgccac accgatcacc aggccatagc cgacgagcac
     1081 cccgagttcc cagaagtggc gggctttttc agcgtagatc accaccgtcg atatcgacgc
     1141 cgggccagtc agcagcggaa tggtcagcgg gaccacggca atgctcgcgc cggaatcggc
     1201 gcgactggcg ccctcgtcga cgtcctgtgc ccgcgactcg gcgggnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnatgca atccctgtgg atgctccttg ccagcctcct
     1381 gtttgcctgc atgggggtgt gcgtgaaact ggtcgccgcc acgcactccg ccgtggagat
     1441 cgtttttttc cgcagcgtcc tgtcgttact cctgatgtat gccctggtcc ggctgcgcgg
     1501 cgtgcccttg aagaccgtgc actggcgctg gcaggtcagc cgcggcgcga tcggtttctc
     1561 ggcgctgtgt gcctacttct gggcaatcac cctgctaccg ctggcgaccg cagtgacgct
     1621 caactatact tcggcgatct tcctcgccgc atatctggcg ctctccggca tgcgcctgaa
     1681 cgtgggaatt atcgcagcgc tggcgctcgg gttgggcggt gtgggtctgc tcctgaaacc
     1741 gaccctgcac gccgaactgt ggatcggcgg cctgattggt ctggcttccg gcgttcttgc
     1801 cggcatggcc tatttcagcg tccgcgaact gggttcgcgt ggcgaaccgg aaacgcgcac
     1861 ggtcttctat ttttcactgg tgtcctcggt atgcgccagc gtctggctgt tgttttgcga
     1921 gatccatccg ttggacctgc gcagcggcgc actgctgttc ggtgtagcgg ctttcgccac
     1981 cgccgcccaa ctggcgatga cccatgctta cgccggtagc cggacgctgt tggccgccgc
     2041 cctggcctac agcacggtcg ttttttccag cgtgttcggg atcctgctct ggaacgaggc
     2101 gcatgacggc tggacctggc tggctatggc actgatcgtg atctccggtc ttgccgccac
     2161 ttatttctcg cccggcagtc cggctacacg gtcgtcggca ccgccagatc agccagcgat
     2221 cgaatcctca ccgagacctg caggcaaaac tcaagcctcc gggaagagcc gcgccagtcg
     2281 aacgtcgtag atcgcccttg cggcctccaa gcagcgcggg ttgccggtag ccagcaggtc
     2341 cgcatagacc agagccggcg ggaccgtgtc gcgctgcccg atgatggaca gggagcggcc
     2401 ccagaacggc ttgcgcactt caagcaggtt ctggtacgcg acggggcctg ccgctgtgag
     2461 tttctgctgg gccaccagtc gcgccgacaa cttgtcggca tagagcgtca gcacgctcgg
     2521 ctgcaggtcg caggcaagca gcgttgccgc cggctcgcca ccccagcgcg ccnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggacgtg accttgcacc ggacttcggc
     2701 ggcgtaatcg gtgccacatc tgtccttgcg cacgcccagg caggtcgccg atcgccagcg
     2761 gccctcgctg ccgtcctgtt tctgtaccga aaccatcagg ccgtgtccgc gcaatgcttg
     2821 cgcggccttt tcgagaagcg tgcttgcttt ttgtgcgttc atggcgctgc cggcgactcg
     2881 tctggtgaac agtccgggat tatgaccgct gcggtttcga tgttcaagcc ggagctggtc
     2941 aatcgacgcc tgccgcgccc gcttcccgga tccgcgatcc actcaccgcc tgcacggtcg
     3001 ccttgcggtc gatcctgccc cagcccacca gcaccccacg cagcgaagtc agggtgctct
     3061 tgatcgccac gtagtacatc agctgccgat agaagaagcg ttgccagaac aaccagatca
     3121 gcagccacca actttccttg cgctccaggg taaaagccag aatggccgcc aggtagtcta
     3181 cggtcacgaa cagcgcgtag tagaacaaca cccgccacaa ggcgtccggg gaatactctt
     3241 ccgggtgttg ccagtgattg aacgccgccg atcccagcga accgatcagc agcaggtcca
     3301 tgatcggcga gaccagcggg aacaggacct ggaacacgat cacgttcggc agggcgacaa
     3361 agcccagcgc gccgtaacgg ggcctgaaca acacgtcgcg atgcttccac gccgcctgca
     3421 tcgttccgta catccagcgg tagcgttgcc ggatgaagcc gcgcacggta tcgggcgcct
     3481 cggtgagggc caccgcctcg tcctcataga cgatcgcgta ccccagtttg cggatcgcca
     3541 gggtcagatc cgcgtcttcc gccagggtca ggtcggtaaa gccgccgacc cgctcgatca
     3601 actctcgccg ccaggcgccc actgcgccag gtaccacggt aatgcagttg agaacgtcga
     3661 aggcccgccg gtccaggttc tggctggtga tgtactccag cgcttgccag cgggtcagca
     3721 gattgatccg gttgccgacc ttggtgttgc cggcgaccgc gccgatcttt ggatcgacga
     3781 aatgccggac cagtttgaca atcgtgtcgc gggtaaacac cgtgtcggca tcgagggcca
     3841 ccacgatctc ggcattggac tgcgccatcc cgaaattgag cgccgccggc ttgccgccat
     3901 tgaccaccga gtacacccgc acgcgcgggt cgccagcaaa agcctcgcgc gccacacgat
     3961 aggtggcatc gctggaaccg tcatcaacga cgatgatctc gagcttccgg ggcgtctcgc
     4021 aagcgagcag cgaggcaatg gtctgaacga tcaccttttc ctcgttgtag gccgggatca
     4081 ccagggtgac cgttggcgaa tattccgggt cgaagacctc gcgacgctgc tgccaacgct
     4141 gataaatggc cagcgtgccg acaaacaaca agcgcgcaat gcccagcacg atgcccagca
     4201 ggaataacca gtggaccgtc gctgccgacc agttgagcac cgcaaaggcc gctccgtcca
     4261 gccagatccg ccagcgggtt tccggtggca ccggcggcat cagcgcgtcg cggctgcgcc
     4321 cgagcagctc ggaaacgctg acgaactgga agccccgtcg ccgcaattcc tcgacgatgc
     4381 gggggatcgc ctgcaccgtc tggctacgat caccgccgga atcatgcaac agcacgatat
     4441 tgccttcacc gcgctccgcc tgctcgacgg tacgccgaac gatctcggcg acccccgggc
     4501 gtttccagtc atccgggtcg atctgcatgc cgaccacgag ataaccctgc tcggcggcca
     4561 gttcgatggg gcgcacctgg tcgatggtct ccggctcggc gtctaccgcg tagggtgggc
     4621 ggaacagcag tgaatggcgg ccgaccacgc tggccagcag atgctgggtc gccgagagtt
     4681 cgagttggaa ctgcatcggc gagatggtcg aaatattggg gtgggtgaag gtgtgattgc
     4741 cgatttcgtg gccttcgtca agcatgcggc gcagcagttc cggatgcatc tgaccattca
     4801 cgcccatgat gaaaaaggcc gccggcactc ccgccaggcg caaggcatcc agcacctgcg
     4861 gggtgtactg aagatcggga ccatcgtcaa aggtcaacac gaccttgcgc tccgcgccgc
     4921 cgtgacgggt gatgacgtag ggtgaaggga aagccgtgaa ccgctcggaa ctgatcagcc
     4981 cgcgcttggg gtcgaattcg ataatccgac tgccggtctg tggttttgcc gtgacttcaa
     5041 ggatctcgcc cttgccttca tagtccacgc cgtaggcaaa gcggatgttg ccgagctccg
     5101 cggcgcgcac cgcatcgagg ggggtatcct tgccgaagat ctgccacagc gccggatcct
     5161 cgctgcccag ccgccacagg gcgacgccgc gcggctgcag ggtacggatt gccagcaact
     5221 ggttgaaggc ggtcacggcg tcgagcatcc acacgtggtg aatgcggtcg ttgtcatcgg
     5281 cataggcgaa agtcggattc agggaagccg gatccagcct gattttgcct tccgactcct
     5341 tggccaccag caccgcctcc tcgaaagttc gctcctcggc gggctggcct ggttgccagt
     5401 cataggcgta attgccaatg gccacgatca tcttctcggc gggtatgtcg cgctgccgct
     5461 gccgcagcac gctggcgaac cacggcaaac tggcgatcgg gccgggcgcg ctggtcgccc
     5521 aatgttcgtc gtaggccatg agaatcaggt aatcggcact acgcaccagg cggcggtaat
     5581 caaacgccgg gtcactggcc gggacgttga ccgagaccag caggtttttc gggtgcatgg
     5641 cggcgtacag ttccgccatg aaacgctgga aatcgggctg ggccttggtc gacacgtttt
     5701 cgaagtcgat gctgatgccg gcaaagcggt tacgctccac ataggcgacc aattgttcga
     5761 tggtgcgcgc ccgcgctgcc ggcctggcca acatgcgtgc cagcttggca ccctcccaat
     5821 cgtgcccgtt ccagttgttg accaggggca cgatcgacac ctcggggcga cgggcgcgga
     5881 cgtattcggt cacctgcgcg gttcgcgtcg gatcgttctc ccgcagcgat ccatcctcgg
     5941 ctgccaaatg cagccactcg gcgatcacca tgtccagact gcccaggttt tccttgagag
     6001 aactcatgct ggagtcgtcc cagttcacaa agaacccgat cgccagagcc tgacgacggg
     6061 caaccgggcc cgccttgcgc gactgcaaca attgtttcag ttcgcgtgcg cgctcgctcg
     6121 ccaatctgtt ctggacatta cgcagggcgc gctcgcggcg ggtcagtgga cgctcgggag
     6181 ccagcgcagg caccgccggc aaggcatgtg cgccttgcgg cagaaagctg acccccgaca
     6241 gattcagcgc cgggagcacc ggactgacga ggatgctcag taccagcgcg cccagcaata
     6301 atgacagggt caggccaatc agcgtcattc ccagccgcag gcgcggccag cgcttgctgg
     6361 ccggatcgaa gaagaccggt ttggcggcgc gcaggcttac cgcccggctg ggcgggcaat
     6421 cgtcaggacg ctgctgcgac atgatgagtt cctggcctgc tgacggccag tgttcgccgc
     6481 tgagggttgg catacactct gccgaggtgc gcgctcgggt tcggctgtcc ggcttgcggc
     6541 gcccggctgc ggactacggt cagggaccac ggtggcaacc gcagatgcag cggcgcctga
     6601 tggccaggca gcaggcggta ttgcggcggg agactgcgct gcgggcgccc gaacgcccca
     6661 ttggcccgcc accgatactg ggcggccgag aactggtaca gatccgccgg gccctgcagc
     6721 ggggtgagcg cgttcgcctc gggaccggag aagtgcagcg caacggtcct ttgccgttgc
     6781 ggatccttgt tgaccagcag taccgaccac aagccatcgg gacgccgcag cgcataggcg
     6841 ctcaccggcg aaacgccgcc cgccgcgtca tcatcgaccc gggtgcgata gacagcgtgg
     6901 gcttgcgtcg ggcttccagc ccattgacga gccagcaacc tggcaccgtg ataggtggcg
     6961 gtgcgcgcca gaatctttcg ccgctggtcg gacaggaaca gggtgttcgt accccaggtg
     7021 tcgcaatccg gccccttgtc cagggggccg ggttcatagc cgtacagata ggcgacggca
     7081 ccgccctcgg tcaggaatgt cccaacgacg tcagcgttga acagcgcgcc gggcagatca
     7141 actgcggtct gcgtcgagaa ggccgagtaa ccgtactcgg tcatcaatag cggcgtgccg
     7201 cgaggcagac cctgttggta aagctccgcc atcgcctcgg tcagcaatcc cggcgcttgc
     7261 cgcaactgtt ccgcgggcgg cgtacacacc tcgtcgaagg gataccattc gaaagacaga
     7321 aaaccgaggt cggcggcgcg ctcccgcacg cgcagatagt cgagcagtcg ggccagccac
     7381 ggtcggtcgg gggcgaccga ggtcccggac caggccatca tcggtgcatc ccacaaggac
     7441 tggaaagacg gtcccccgag gttcacgtcc ggatcgaccg cgcgcaaggc atcggtcacc
     7501 tgcaggtaga gggcgccata gtcttccgga gccacgtact ggccatccgg ttcctcgccc
     7561 aactcgacac cgcgaatcgg ataagcgcgt cgccgcaggt agcgcaggaa cgcagcggcg
     7621 ttgtccggag tatcgtacag cacaccgacc ggaaccagca tcggcagacc gtggccgagc
     7681 ccgctggtga acacgcggtc gatgccggcc tgttccacct ccgggtcgag atccttggcg
     7741 cgatgccagg ggtcggtcga cgacacaaac atccgggtct ggccgtcgtg gctggcggcg
     7801 tgtcgtacgg catcgtgcaa ccggtcgttc gcgtcgagcg cccccacata gacctcgcgc
     7861 accgcgtagc ccagtgcatc gcgcgcatcg cgcgcaccag gcgctgggct gccggacgag
     7921 acttccagca gaacacgcaa ataacgcacc gccaccgcgt gccgggccaa tcgcagattt
     7981 tcatcgccac cactaccctg tctgacctct ccctcgggga aagtctgcca gtgcccgctc
     8041 ggatactcgt ccgggagggc agcgtcttcg ccatcccaat actgcacctg atagcgcacg
     8101 gcatacggca ttccccaggc gatgcgcatc gcgtccaccg gcatcttctt tccgagatcg
     8161 acgatcagcc actgtggatg gcggctgttg tcctcaccag tgaaaccgcg gtccagatag
     8221 ggattgctct tccagaaggt gtcgagatcg ccgtcggcca gtcgcgagta accgttgtct
     8281 tcggcctggt cgacggtgta tccgcggcgc ggcaaacggt agccaaacga cgccagaatc
     8341 ggcgcgccgg gttggtcgtc ggaagtccag tagcctcgcg cgtgctcgct gtcgctccag
     8401 cggccgcgtg gattccagtg ccaggcctcg acgcccaact cggtacgcag acgatagctg
     8461 agtggacgca ggcctaccga gcgcatggca cgcaggttcc tcggtcggta gatggctgcc
     8521 gcatcaccgc gcgagtgccc atcgaccccg gcaccaagcg caaccgcggg cacaaaggtg
     8581 ttgctcggtc cactggtcag gtcaatggtc accctggccg gtgcctccgc tccgaaactg
     8641 gatagcggac tggccagggt catgacaagg atcattgcgg cagtctgacg ggaggagaaa
     8701 tcatgcattc ttgcgtactc gctgaaacgg ccgtcgcctg ttcctccagc cgttccccgc
     8761 ctggacggag ccgcattata ctcacgccga tggggtgacg tgcgcctcgc cacccgttgc
     8821 cggaaaatag cagatcctgc gccaacatcc cttcagcatg gtgatgaaaa aggccgacag
     8881 gggtaggctg gccttttcga acctcacccg cccaccgccg atggcttcca gcaaggccag
     8941 gccacgcccg cgaacgaggt aactgtcgcc cgcagccagg aagatgtcac cggcctctcc
     9001 ggcgatggtc agccacacca cgcctgatgt gcagcgaatt cgcacccccc gggctgatag
     9061 caggcgaatg ggtgcgtcgt cggcgaggca aagctcactg ttgaagaggt cgaggtccat
     9121 ggcggtttct ccttgtgttg acgtcgagga gtgtagggca gttggcctaa agggtacagt
     9181 tacaatataa tataattgta accggtacaa ttttgaatct ggcgaattgt actggccatt
     9241 gcgcgtcgta actgtactgg tgaggctgat ggaaaacgaa ctcttgcgtt acgaacggct
     9301 ggccgaggat ctgggcggca tcatcgtggc cggcaacctg cggcccggcg agcgtttgcc
     9361 gtcggtgcgg cgcctgtcgc gcgagcggcg gctctccgta tcgaccgtgc tgcaggcctt
     9421 gcgccggctc gaagaccgag gcctggtcga ggcgcggccg cagtcggggt acttcgtgcg
     9481 ccatacggca gcacgaagat cacaaccgaa cgccaggtca accccggaag cgccggtacc
     9541 ggtcgatgtc agccagcggc tgatgcgggt attgcagacc agcatgcaac ccggggtcac
     9601 cccgctggcg gccgccctgc ccgcgtgctc cctgttgcca ctggctgccc tgcagcgact
     9661 ctacggcggc gtcgcacgcc gacatcccca cctgctcgaa ggcggcagcc atgtcaacat
     9721 ggatacgcca gcgctggtcc gtcagcttgt cttacgctcg gtaggctggg ccggaccact
     9781 ggccgcgagc gagttcgtga tcaccaattc ctgtaccgaa gccctgggac tctgcctgcg
     9841 ggcggtaacg caaccgggag acacggtggc cgtcgaatca ccggcttatt acctgatgct
     9901 gcagttgctc gaaacgctcg ggctgaaggc gctcgaaatt cccaccgacc cgcacagcgg
     9961 cctgtcgatc gaggcccttg atctggctac tcgccaaggc aaggttgctg cttgtctgct
    10021 ggtgcccaac gccagcaatc cattgggcag catcatgccg gacgagca
//
LOCUS       DAOSIT010000015         9834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6665_length_9834_cov_11.004193, whole genome shotgun sequence.
ACCESSION   DAOSIT010000015 DAOSIT010000000
VERSION     DAOSIT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6665_length_9834_cov_11.004193"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLL14_00870"
     CDS             complement(<1..221)
                     /locus_tag="PLL14_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOG02496.1"
                     /translation="MSHAICRHLRISGRVQGVGYRWSLCAEAERLGLSGWVRNRRDGS
                     VEALLSGPIEAVDALTLWAQRGPSMARVD"
     gene            343..1815
                     /locus_tag="PLL14_00875"
     CDS             343..1815
                     /locus_tag="PLL14_00875"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG02497.1"
                     /translation="MLKALLLLPLLGAAVLSLLPSRSVDLLRHLASLVAAATMVCAFL
                     LLVDFDSGSAEIQFFDTRPWNPRVGSHLALGVDGVSLPMVLLATVLCFVAIFSSTGIR
                     SGARLYFSLLLVLETSLLIVFTARDWSLFYVCWELTLIPLFFLIDRLGGGNRHRAALN
                     FVLYTLGGSVFMLIALLLLYDVAPGHSFAMADMAAGGAQLPVRTQILVFLGLLLGFGV
                     KMPIVPLHGWLPLAHVEAPSAVSILLSGILLKMGAYGLIRAAATLPAAVQVLQNGLAL
                     LAMVSLLYGAVLAWRQTDLKAMIAYSSISHMGVVLLGIAALNPAGLSGALTQMVAHGL
                     VAGLLFLLIGLLYERTHTRDLAGYGSLNRIAPRFAVFMVFTLLASVGLPGSAGFIAEL
                     QVLVGAYGRWGGWLMVLSMALLVSAAYALRAIRCLSTGPERPFGTTPAGVAHFADLKR
                     SECAAAWLLTAGIVGIGFYPAPLLRLMASSVGRLAQHFGV"
     gene            1816..5013
                     /locus_tag="PLL14_00880"
     CDS             1816..5013
                     /locus_tag="PLL14_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HOG02498.1"
                     /translation="MSSPVPSQTTAASVAAVRHVQTCLQHLAHLLPAQAPLRDFVHHN
                     TLHAFQHLPFADALKEAARLSGARPWLDENRCRELFRQGRVDAGDLDAALRQSAQTRV
                     DELLGVGTPIALRRGDVLRAALLYPPVAISAPRLRWQREEKGAFERLQADLDPGARQR
                     LLSTAAADGDDERAAVADLWAAACALGARPPVSGWVAGGVDATPESAVALWHELLDRL
                     GGEWTVASLLCHLTGEDFRQSLQPALIRHLAAHLDQGLAAWKNPAQSQGFYAAWRACA
                     GSDWAWELDEFAGARREIEALPDDPLQAMASELLRLGIDEARTGAYLQRLALELPGWA
                     GMFHWRESHAAGDDAPVRLADLLAVRVILERLHAEHLVHRVWGLPLPLAELGEYFARH
                     PAELAVRHACGNRQLPEELLAAVRPQLAWPSADVHEGWRHVAVALAEAHNAGMDDRER
                     AELAAWPLFVLAQGLGLCGRELRALGAEGAAALLRCAGSLDDEQRGEVWLLAYEHHYR
                     RQILAALQANHGRAPVPTGTAQFVFCMDDREEGTRRHLEEVNPAVETFGAAGFFGLPM
                     LWQGLDDEAPTALCPVVVRPEHAVREAVLPGCEAAQRVHQRRRRWRLAWRERLHQGSR
                     RGALAAALLIAAAAPWALLGLLARTLAPGRLGAWLVRTRDAFDLPVPGGLQLTAEADE
                     ATRVASPEAPRLGFGEAEQVARIGGFLRSIGLTTHFAPLVVIVGHGSDSRNNPHLAAY
                     DCGACSGRHGGPNARVFAAMANRPTLRASLAQQGIVIPATTYFVSAEHNTCDETFVWY
                     DLALLPSSHRQAFAALRKDCEEALRLHAVERCRRFASAPLAPLPSQARQHLADRRHDI
                     GQARPELGHATVAAAFVGRRAMSRGAFFDRRVFLISYDPLDDADGRVLEATLLAAGPV
                     GAGINLEYYFSTVNNEGYGCGSKVTHNLAGLFGVMQGASSDLRTGLPLQMVEVHEAMR
                     LLVVVEQQLEIVSAIYQRQPPLQELIGNRWVVLAAKHPQSGAIHLFDPASGWQPWNDS
                     AGQPAALPAVERSADWFAGRRDPLSPALLRRPVSA"
     gene            5013..6563
                     /locus_tag="PLL14_00885"
     CDS             5013..6563
                     /locus_tag="PLL14_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG02499.1"
                     /translation="MDWLEALLPALATTTRLVWLVPLLPLLAAAAIGAGLLLRRFNGD
                     AGEPLIAAMASAGALAALLLLLVFDLAALGGAAFEPLVFGEWLRIGAIPVRLSFVLDA
                     YSLPAATLVALVAWLALRFSATYLHRESGFPRFFLGMCLFLAGMLLIVLSANAVLAFV
                     GWELAGISSWLLIAYNWERPLATGNALFVFLASRVGDAGFMLGIGLAVWSAGTVEWLW
                     LAGDGSMATVTARLLAAGFVLAALVKSAQLPFTPWIARALEGPTPSSAVFYGSLMVHA
                     GVYLLCRLQGLLLQVPDLLALLAVVGLTTALYGGLCALVQSDVKSALIFSVVAQVGLM
                     FFCCGLGLFWLAAVYSGLHAAWRAYQFLLAPAYMHLVRRPARPLPRWLAAQRWCYTAV
                     LQRFWIEALANSLLTRPTLALGRDVRALDERFIDPLVGAPRDDEPVLGTDASDQLIRG
                     HGLAGRALFAFANRLQRLESRLLLSGDGALSRVLRRGVSYADAIESLLEQPRYLMLMV
                     MATLVVIL"
     gene            6560..8059
                     /locus_tag="PLL14_00890"
     CDS             6560..8059
                     /locus_tag="PLL14_00890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG02500.1"
                     /translation="MNEVLWAAQAGYPPLATLQWLPLAAAVLLMIMREDDLALVLGRL
                     FAVAELVVAIDLYARIDVTRNVLQFAERLDVFAYHAAADGVSVLFILLTALLGVLLSF
                     YGMARQQLTPVQLLSVLLLIESSQMVMLTTMNLLWFAAASAVQLALVAYLLWRWASAR
                     EENLALSRFLQYQLFGWCLFVAGAVVLVWSHADVLGGHWSFDLFDLLQTPQIGKYQSV
                     AFYLLFYGLAIRTPLFPLHGWLPNSAGYGLIAVGPVLLLGVKVGIYGMARFLLPLTAE
                     AVMIWQSYVVAFAMVGVFFAAIMAFRQANLRRLMAFAVVSHTSLIVIGLFTLHPAGLQ
                     GALLLAVNFGLAITLMLLMVGYVYRRTGTTSLDRLGGLFDRVPFIAVAFLIGGLSLLG
                     MPGTPGFDAVHLVLEAAIETFGALPTIATALGNVAAAGFLLWAFQRAFLAPRPRDLPA
                     ARIARTARMEYFVGGATLLVLLAAGFYPEPWLQLTDTATQALAAYFQHG"
     gene            8052..9548
                     /locus_tag="PLL14_00895"
     CDS             8052..9548
                     /locus_tag="PLL14_00895"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOG02501.1"
                     /translation="MAELAAWPLLSILLALPVAGALLTALLRRAPWAQWIALGTATMT
                     LLCALLIVAAFDRADPDFQLLDSADWVSGLGVRYLVGVDGLSLLFLPATALLFCGAIV
                     AGWRVRAAASPLAPGLYFALLLLLEAATLGVFCALDSILFFFCWEFTLLPLYFLVSLW
                     GTGAGRQAAAVRYFLVMLAGGVPLLFGLLALVFGQAAGGALAFDLPTLLATPLPESRQ
                     YLVFLLLLVGFGVKVPLVPLHTWLPSLAMGAPAAVTALLVGLKLGAYGLIRFAIPLAP
                     IAARDLHWLLAGFGTVAILYGGVAALAQSNLRSILAYASLAHVGLVLLGLAAFSVSAL
                     QGVLLQLLNFSLSAGGSFLALAFLQRRTASTDISQLGGLLRSMPVLSGFFLLFGLAGI
                     GLPGTSGFPGELLIIVAALHTHTGAGLAALFGMVLAAAGFLAPFRQAFLGPTRNPDVA
                     DADDLLPREVAVLLVPGLLILAIGVYPLPVLELIRPAAEAWVAALAGL"
     gene            complement(9601..>9834)
                     /locus_tag="PLL14_00900"
     CDS             complement(9601..>9834)
                     /locus_tag="PLL14_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02502.1"
                     /translation="VLERLGWQFVRIRGSAFFRNPEAAMRPVFDRLAALAIPPAVDVD
                     IHAAQGEDMTLIHELEALKKGKVAIGGDDIRLL"
BASE COUNT         1187 a         3297 c         3354 g         1996 t
ORIGIN      
        1 ctgtcgaccc tggccatcga ggggccacgc tgcgcccaga gggtcagcgc gtcaacggcc
       61 tcgatggggc cgctgagcaa cgcctcgacg ctgccgtcgc ggcggttgcg tacccagccc
      121 gacaggccga gccgttcggc ctcggcgcac agcgaccagc ggtagcccac cccctggacg
      181 cggccgctga tgcgcaggtg acggcagatt gcgtggctca tcttcaactc cggactctgg
      241 acaagggatg cgagcgtcgc cattactggc ataatcgtcg tccatgagag caagaatatc
      301 aagggggggg cggctcaaac ccctcgctgc cgctgcccga cgatgctgaa agccctgctg
      361 ttgttgccgc tgcttggcgc cgccgtcctg tcgctgctgc cgagccgcag tgtcgatcta
      421 ctgcgccatc tggcgagtct cgtcgctgcg gcgaccatgg tctgtgcgtt tctgctgctg
      481 gtcgattttg attcgggcag cgccgaaatc cagttcttcg acacgcggcc ctggaatccg
      541 cgagtcgggt cgcacctggc gctcggcgtc gatggcgtgt ccttgccgat ggtcttgctg
      601 gcgacggtcc tgtgtttcgt cgcgatcttc tcgtcgaccg gcattcgcag cggcgccagg
      661 ctctatttct ctcttctgct ggtgctcgaa acctcgctgc tgatcgtgtt cacggcgcgc
      721 gactggtcgc tgttctacgt ctgctgggaa ttgacgctga tccccctgtt cttcctgatc
      781 gaccgtctgg ggggcggcaa tcgtcatcgc gcagcgctca atttcgtcct ctatacgctc
      841 ggcggctcgg tgttcatgct gattgccttg ctgctgctct acgatgtcgc ccccgggcac
      901 tcttttgcaa tggccgacat ggccgcaggt ggcgcgcagt tgccggtacg cacgcagatc
      961 ctggtctttc tcggcctgtt gctgggcttc ggcgtcaaga tgccgatcgt gccgctgcat
     1021 ggctggttgc cgctcgccca cgtcgaggcg ccgagtgcgg tgtccatcct gctctcgggg
     1081 attctgctga agatgggcgc ctacgggctg attcgcgccg ccgcgacctt gccggcggcg
     1141 gtgcaggtcc tgcagaacgg gctggcgctg ctggccatgg tcagcctgct ttacggtgcc
     1201 gttctggcct ggcggcagac cgacctgaaa gcgatgatcg cctactcctc gatttcgcac
     1261 atgggtgtcg tgttgctcgg catcgccgcg ctcaaccccg ccggcctcag cggcgcgctg
     1321 acgcagatgg tcgcgcatgg cctggtcgcc ggcctgctgt ttctgctgat cggcctgctc
     1381 tacgaacgca cgcatacccg agacctcgcc ggatatggtt cgctcaatcg catcgccccg
     1441 cgtttcgcgg tgttcatggt gttcaccctg ctggcgtcgg tcggtctgcc gggtagcgcc
     1501 ggtttcatcg ccgaactgca ggtgctggtc ggcgcttatg gccgctgggg cggctggctg
     1561 atggtgctgt cgatggcttt gctggtctcg gcggcctatg cattacgtgc catccgctgc
     1621 ctgtcgaccg gccccgagcg tccgttcggc acgacgccgg cgggggtggc gcacttcgcc
     1681 gacctgaagc gctcggagtg cgccgcggcc tggctgctga ccgccggcat tgtcggcatc
     1741 ggtttttatc cggcccccct gctgcgcctg atggcgtcgt cggtgggccg gctggcacag
     1801 cattttggag tctgagtgag ttcgccggtg ccttcgcaaa ctaccgccgc gtcggtggcc
     1861 gcagtgcggc acgtgcagac ctgcctgcaa cacctcgcgc atctgttgcc ggcgcaggcg
     1921 ccgctgcgcg attttgtcca tcacaacacc ctgcatgcct tccagcatct gccttttgcc
     1981 gacgccctga aggaggccgc gcgtctgagc ggcgcgcgtc catggctcga cgaaaatcgc
     2041 tgtcgcgagc tgttccggca ggggcgcgtg gatgccggcg atctcgatgc cgccttgcgg
     2101 caatcagcgc agacacgggt tgacgaactg ctcggtgtcg gcacgccaat cgcgctacgg
     2161 cgcggcgatg tcctgcgggc agcgctgctg tatccgccgg tggccatttc cgccccgcgc
     2221 ttgcgctggc aaagggaaga gaagggcgct ttcgaacgac tgcaagccga cctggacccg
     2281 ggcgcgcggc agcgcctgct gagcacggcg gccgctgacg gtgacgatga gcgtgcggcc
     2341 gttgcggacc tgtgggcggc ggcatgcgca ctgggcgcgc ggccgccggt gtccggctgg
     2401 gtcgccggcg gcgtcgacgc gacgccggaa tctgccgtcg ccctctggca cgagttgctc
     2461 gaccgtctgg gcggagaatg gacggtggcg agcttgctct gccacctgac cggcgaggat
     2521 tttcgccagt cgctgcagcc ggcgctgatc cgtcatctcg ccgcgcatct cgatcagggc
     2581 ctcgccgcct ggaaaaaccc ggcgcagtcg cagggtttct acgcggcctg gcgagcgtgt
     2641 gccggtagcg actgggcctg ggaactcgac gagtttgccg gcgcgcggcg tgaaatcgag
     2701 gcactgccgg acgacccgct gcaagcgatg gcgagcgaac tgttgcgcct cggcatcgac
     2761 gaagcacgca ccggcgccta tctgcagcgg ctggcgctgg aattgccagg ctgggccggc
     2821 atgttccact ggcgggaatc acatgccgcc ggcgacgacg cgccggttcg gctggccgat
     2881 ctgctggcgg tacgggtgat cctcgaacgt ctgcatgccg aacatctggt gcaccgcgtc
     2941 tggggtttgc cgctgccact cgccgaactc ggcgagtact ttgctcgtca cccggccgaa
     3001 ctggccgtgc gacacgcctg cggcaaccga cagttgccgg aagaactgct cgccgcggtg
     3061 cgcccgcaac tcgcctggcc gtctgcggac gttcacgagg gctggcggca cgtcgccgtc
     3121 gcgcttgccg aggcccacaa cgccggtatg gatgaccgcg agcgtgcgga actggcggcg
     3181 tggccgctgt tcgtcctggc gcagggtctg gggctttgtg gccgcgaact gcgtgcgctc
     3241 ggggcggagg gcgcggcagc cctgctgcgc tgtgccggct cgctcgatga cgagcagcgt
     3301 ggcgaggtct ggctgctggc ctacgagcac cattatcggc ggcagattct ggcggcgctg
     3361 caggccaacc acggccgcgc gccggtgccg accggcacgg cgcagttcgt gttctgcatg
     3421 gacgatcgcg aggaggggac gcgacgccac ctcgaagagg tcaatccggc ggtcgagact
     3481 ttcggtgcgg ccggattctt cggcttgccg atgctttggc aaggtcttga cgacgaggca
     3541 ccgacggccc tgtgtccggt cgtggtgcgt cccgagcatg ccgtgcgtga agcagtgctg
     3601 cccggctgcg aggcggcgca gcgcgtgcat caacgacgtc gccggtggcg tctggcatgg
     3661 cgggagaggc tgcatcaggg gagtcggcgc ggcgccctgg cggccgcgct gctgatcgcc
     3721 gccgccgcgc catgggcgct gctcgggctg ctggcgcgaa cactggcgcc ggggcgcctg
     3781 ggggcctggc tagtgcgcac ccgggacgct ttcgacctgc cggtgccggg cggcctccag
     3841 ctgactgccg aagccgacga agcgacgcgt gtcgccagtc ccgaggcgcc gcggctcggc
     3901 ttcggagaag cggagcaggt cgcgcgcatc ggtggcttcc tgcgcagtat cgggttgacg
     3961 acgcacttcg caccgctggt ggtgatcgtc ggtcacggct cggacagccg caacaacccg
     4021 catcttgccg cttacgattg cggtgcctgt tccggccgcc atggcgggcc gaatgcacgc
     4081 gtgttcgcgg cgatggccaa ccggccgacg ctgcgcgcaa gcctcgcgca acagggtatc
     4141 gtgattccgg cgacgacgta tttcgtcagt gccgaacaca atacctgcga cgagaccttc
     4201 gtctggtacg accttgcgct tcttccgtcc agccaccgcc aagcctttgc cgcgctgcgc
     4261 aaggattgtg aagaggcgct gcgcctgcac gctgtcgagc gctgtcgccg cttcgcctcg
     4321 gcaccgcttg ctcccttgcc gtcacaagca cggcagcacc tcgccgaccg gcggcacgac
     4381 atcgggcagg cccgccccga actcgggcat gcgaccgtcg ccgcggcgtt cgtgggacgt
     4441 cgcgcgatga gccgcggagc gtttttcgat cgtcgcgttt tcctgatctc ctacgatccg
     4501 cttgacgatg ccgacggccg cgtgctggag gcgacgctgc tggcggcggg tccggtcggg
     4561 gcgggaatca atctcgaata ttacttttcg acggtgaaca acgagggata tggctgtggc
     4621 agcaaggtca cacacaacct cgccggcctg ttcggggtga tgcagggcgc cagttcggac
     4681 ctgcgcaccg gcctgccctt gcagatggtc gaggtgcatg aggcgatgcg gctgctggtg
     4741 gtcgtcgagc agcaactgga aatcgtcagc gcgatctacc agcggcaacc ccccctgcag
     4801 gaactgatcg gcaatcggtg ggtcgtcctg gcagccaagc acccgcagag cggcgcgatc
     4861 cacctcttcg atccggccag cggctggcag ccctggaacg acagtgccgg gcagccggcg
     4921 gctctgcccg cggtggagcg ttcggccgac tggttcgccg gtcgccgcga tcccctctcg
     4981 ccggccttgc tgcgccgccc ggtgagtgcc tgatggactg gctggaagcc ctgttgccgg
     5041 cgctcgcgac gacgacacga ctggtctggc tggtgccgct gttgcccttg ctggccgctg
     5101 cggcaatcgg cgctggcctg ctcctgcggc gattcaatgg cgacgccggc gagccgctga
     5161 tcgccgcgat ggccagtgcc ggcgcactcg ctgcgctgtt gctgctgctg gtttttgacc
     5221 tggcggcgtt gggcggtgcc gcgttcgaac cgttggtctt tggcgagtgg ttgcggattg
     5281 gcgcgatacc ggtgcgcctg tcattcgtgc tcgacgccta cagcctgccg gcggcgacgc
     5341 tggtcgccct ggtcgcctgg ctggcgctgc gcttttcggc gacctacctg caccgcgaga
     5401 gcggtttccc gcgttttttc ctcggcatgt gcctgtttct ggccggcatg ttgctgatcg
     5461 tcctttccgc caacgccgtg cttgccttcg tcggctggga actggccggc atcagctcgt
     5521 ggctgctcat tgcctacaac tgggaaaggc cgctggccac cggcaacgcc ttgttcgtct
     5581 tccttgccag ccgtgtcggc gacgccggtt tcatgctcgg gatcgggctt gcggtgtggt
     5641 cggcggggac cgtcgagtgg ctctggctgg ccggcgacgg cagcatggcc acggtcacgg
     5701 cccgcttgct ggcggccggt ttcgtgctgg cggcgctggt caagtcggcg caactgccat
     5761 tcacgccctg gattgcacgt gccctcgaag gcccgacacc gtcgtcggcg gtgttctacg
     5821 gctcgctgat ggtgcacgcc ggcgtctatc tgctctgtcg tctgcagggg ctgttgctgc
     5881 aggtgcccga cctgctggcc ctgctggccg tcgtcggtct gacgaccgcc ctgtacggtg
     5941 gcctctgcgc gctggtgcag agcgacgtca agtcggcgct cattttctcg gtcgtggcgc
     6001 aggtcggatt gatgttcttt tgttgtggtc tcggtctgtt ctggctcgct gcggtgtatt
     6061 ccggcctgca tgcggcctgg cgtgcctacc agttcctgct cgccccggcg tacatgcacc
     6121 tggtgcgtcg tccggcgcgg ccgttgccgc gttggctggc cgcgcagcgc tggtgctaca
     6181 ccgcggtgtt gcaacgcttc tggatcgaag ctctggccaa cagcctgctg acccgcccga
     6241 ctctggctct cggccgcgac gtgcgggcac tcgacgaacg tttcatcgac ccgctggtcg
     6301 gcgcgccgcg cgacgatgaa ccggtgctcg gcacggacgc gagcgaccaa ctgatccgcg
     6361 gtcatggtct ggccgggcgc gcgctgttcg ccttcgcgaa ccgcttgcag cgcctggaga
     6421 gtcgcctgct gctcagtggc gacggtgcgc tgagccgggt cctgcggcgt ggcgtcagct
     6481 acgcggatgc catcgagtcg ctgctcgaac aaccgcgtta tctgatgttg atggtcatgg
     6541 cgacgctggt ggtcattctg tgaacgaagt cctctgggcc gctcaagccg gttacccgcc
     6601 gctggcgacg ctgcaatggt tgccgctggc tgccgccgtg ctgctgatga tcatgcgcga
     6661 ggatgacctg gcgctggtcc tcgggcgcct gtttgcggtc gccgaactgg tggtggcgat
     6721 cgatctctac gcccgcatcg acgtgacccg caatgtcctg cagtttgccg agcgcctcga
     6781 tgtgttcgcc taccatgccg cggccgacgg cgtcagcgtg ctgttcatcc tgctgacggc
     6841 gctgctcggc gtcctgctgt cgttctacgg catggcgcgc cagcagctca cgccggtgca
     6901 actgctcagc gtcctgctgc tcatcgaatc gtcgcagatg gtgatgctga cgaccatgaa
     6961 tctgctgtgg tttgccgccg cctcggcggt gcagctggcg ctcgtggctt acctgctctg
     7021 gcgctgggcc tcggccagag aggagaacct cgccctgtcg cgttttctgc agtatcagct
     7081 cttcggctgg tgcctgttcg tcgccggtgc cgtggtgctg gtctggagcc acgccgacgt
     7141 cctcggcgga cactggagct tcgatctctt cgatctgctg cagacgccgc agatcggcaa
     7201 gtaccagtcg gtggcgtttt acctgctgtt ctacggcctg gccatccgta cgccactgtt
     7261 tccgctgcac ggctggctgc cgaattccgc cggctatggg ctgatcgccg tcggcccggt
     7321 gctcctgctc ggcgtcaagg tcggcattta cggcatggcg cgcttcctgc tgccgctgac
     7381 cgccgaagcg gtcatgatct ggcagtccta cgtcgtcgct tttgccatgg tcggcgtatt
     7441 ctttgcggcg atcatggcct tccggcaggc caacctgcgc cgcctgatgg ccttcgctgt
     7501 cgtcagccac accagtctga tcgtgatcgg tctgtttacg ctgcatccgg ccggcctgca
     7561 gggggcgctg ctgctggcgg tgaacttcgg gctggcgatc acgctgatgc tgctgatggt
     7621 cggctacgtc taccgccgca ccggtaccac cagcctcgac cgcctcggcg gcctgttcga
     7681 tcgcgtgccc ttcattgccg tcgccttcct gatcggtggc ctgtcgctgc tcgggatgcc
     7741 ggggacaccc ggtttcgacg ccgtgcacct ggtgctcgaa gccgccatcg agaccttcgg
     7801 cgccttgccg acgatcgcca cggcgctggg caatgtggcc gctgccggtt tcctgctgtg
     7861 ggctttccag cgcgcctttc ttgcgccgcg gcccagggac ctgccggccg cgcgcatcgc
     7921 acgtaccgcc cgcatggagt atttcgtcgg tggcgcaacc ctgctggtgt tgcttgccgc
     7981 cggcttctac cccgagccct ggctgcaact gaccgacacc gcgacgcagg ccctggccgc
     8041 ctattttcaa catggctgaa ctcgccgcct ggccactgct gagtatcctg ctggcgctgc
     8101 cggtggccgg tgcgctgctg acggcgctgc tgcggcgcgc cccctgggcg cagtggatcg
     8161 cgctcggcac cgccacgatg acgctgttgt gcgcactgct gatcgtcgcc gccttcgacc
     8221 gcgccgaccc ggacttccag ttgctcgatt cggcggactg ggtgagcggg ctcggcgtcc
     8281 gctatctggt cggtgtcgat ggcctctcgc tgcttttcct gccggcaacg gcgctgctgt
     8341 tctgcggcgc gattgtcgcc gggtggcggg tcagggcagc cgcctcgccg ctggcgccgg
     8401 gcctgtactt tgcgctgctg ctgttgctcg aagcagccac gctgggcgtc ttctgcgcgc
     8461 tcgacagcat cctgttcttc ttctgctggg agttcaccct gctgccgctg tacttcctgg
     8521 tcagcctgtg gggcaccggg gcgggccgcc aggcggccgc ggtgcgctat ttcctggtca
     8581 tgctggccgg tggcgtgccg ctgttgttcg gtctgctggc gctggttttc gggcaggctg
     8641 ccggcggtgc cctggcgttc gacctgccga cgctgctggc gacaccgctg cccgagtcac
     8701 gacagtatct ggtgttcctg ttgctgctcg tcggcttcgg cgtcaaggtg ccgctggtgc
     8761 cgctgcacac ctggttgccg tcgctggcca tgggcgcgcc ggcggcggtc accgcgctgc
     8821 tggtcgggct gaagctcggc gcctatggcc tgattcgctt cgccatcccg ctggcgccga
     8881 tcgccgcgcg cgacctgcac tggctgctcg ccggtttcgg cacagtggcc atcctctacg
     8941 gcggcgtcgc cgcgctggcg cagagcaacc tgcgcagcat cctggcgtac gcgagcctgg
     9001 cgcatgtcgg actggtgctg ctcggcctgg ccgcgttctc ggtttcagcg ctgcagggcg
     9061 tgctgttgca actgctcaat ttcagcctgt ctgccggcgg cagcttcctg gcgctggcct
     9121 ttctgcagcg ccgcaccgct tccaccgaca tcagccaact cggtggcctg ctgcgcagca
     9181 tgccggtgct gtccggtttc ttcctgctgt tcgggctggc cggtatcggc ctgccgggca
     9241 ccagcggctt ccctggcgaa ctgctgatca tcgtcgccgc gctgcacacc cataccggcg
     9301 ccggccttgc cgccctgttc ggcatggtgc tcgccgccgc cggctttctg gcgccgttcc
     9361 ggcaggcctt tctcgggccg acgcgcaatc ccgacgtggc cgacgccgac gacctcttgc
     9421 cacgcgaggt ggcggtgctg ctggtacccg gcctcctgat tctggcgatt ggcgtctatc
     9481 cgctgccggt gcttgaactg atccggccag ccgccgaggc ctgggtggcg gcgctggcgg
     9541 ggttgtaggc ggccgggctc gtgctggttc gagtgtacgc aagaaacgcg ttcctggggt
     9601 tcaaagcagc cgtatgtcgt ccccgccgat cgccaccttt ccctttttca aggcctccag
     9661 ttcatgaatc agtgtcatgt cctcaccctg cgcggcgtga atgtcgacat cgacggccgg
     9721 gggaatcgct aacgccgcca gtcgatcgaa taccggccgc atggcagctt ctggattgcg
     9781 gaagaaggcg ctgccgcgga tacggacgaa ttgccagcca agccgttcga ggac
//
LOCUS       DAOSIT010000016         9788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6700_length_9788_cov_11.564060, whole genome shotgun sequence.
ACCESSION   DAOSIT010000016 DAOSIT010000000
VERSION     DAOSIT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6700_length_9788_cov_11.564060"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLL14_00905"
     CDS             <1..722
                     /locus_tag="PLL14_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG02503.1"
                     /translation="GDGVMVLNRDDVRSLAAARPDRELVTFGLDPATRVGDYGVVDGC
                     IVRDGEKLLALDELRLVGRHNAANAMAALALCEAVGIQAGSVLPALLAFRGLAHRVEW
                     VAEINGVGYFDDSKGTNVGATLAAIEGLGRRVAIILGGDGKGQDFSPLRHAIEQHARG
                     VALIGRDASEIAAVLAGCQVPQRVCADMAEAVRWCAAQAQQGDAVLLSPACASMDMYR
                     DYAHRAEVFVDAVRRIEREAA"
     gene            722..1882
                     /gene="ftsW"
                     /locus_tag="PLL14_00910"
     CDS             722..1882
                     /gene="ftsW"
                     /locus_tag="PLL14_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOG02504.1"
                     /translation="MLHTLDAPRRLPSEVDLALLWSTLMLLVIGMVMVYSASMAIAEA
                     GRQTGNQSAYFLIRHAVFLVVALVASMVAFQVPLATWQRWSPWLFVGGFVLLALVLIP
                     GIGRDVNGARRWLPLGIANLQPSELMKLFAVLYAADYTARKMPHMHDLKKAFLPLASA
                     MVAVGILLLKEPDFGAFVVIISIAMGILFLGGMKARLFVVLIIVLGAAFAALIIFSPY
                     RRDRIFGFMDPWADAFGRGYQLSHALIAFGRGELLGVGLGASVEKLFYLPEAHTDFLL
                     AVIAEELGFVGVLVVILLFGLLIQRAFAIGRQAVALERLYSALVAQGIGVWLGVQAFI
                     NMGVNMGLLPTKGLTLPLMSFGGSGILANCVALAVLLRIDWENRQLMRGAKV"
     gene            1896..2954
                     /gene="murG"
                     /locus_tag="PLL14_00915"
     CDS             1896..2954
                     /gene="murG"
                     /locus_tag="PLL14_00915"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOG02505.1"
                     /translation="MNRTLLVMAGGTGGHVFPGLAVADLLKSRDWRVVWMGAPDGLEA
                     KLVPPRGYEMAWVRFAALRGKGLLRKLLLPFHLFAACWQARREIRRVRPDVVLGMGGY
                     VSFPGGMMAALLGCPLIIHEQNSIAGLANRVLARFARRVACGFPNALPSGVWVGNPLR
                     PEMSRVPAPAQRLAGRQAPLHLLVLGGSQGAAALNDIVPRGLALIDSEQRPLVVHQAG
                     EKHLAQLRQNYEAAGVQAHCAAFIDDMAGAYEWADLVICRAGALTVAELAATGVASVL
                     VPFPYAVDDHQTHNAIFLSQAGGAILLPQDQMTPESISEIRNYPRSQLQEMAEKSHEL
                     ARPEATAELARMCEELAQ"
     gene            2951..4339
                     /gene="murC"
                     /locus_tag="PLL14_00920"
     CDS             2951..4339
                     /gene="murC"
                     /locus_tag="PLL14_00920"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOG02506.1"
                     /translation="MKHKVKNIHFVGIGGAGMSGIAEVLANLGFSVSGSDLAESSTTR
                     RLGSLGVRIMIGHAAANVDAAEAVVVSSAVKSDNPEVLAARRRKVPVVPRAQMLAELM
                     RLKQGIAVAGTHGKTTTTSLVASILAEGGMDPTFVIGGRLNAAGANARLGSGDFLVAE
                     ADESDASFLLLSPVISIVTNIDADHMETYGHDFSRLKQAFVDFLQRLPFYGVAVLCAD
                     DVNVREIMPLVSKQIVSYGFDASANIYAENLTAVAGQMHFDCVRVNGSVSRLPVILNL
                     PGRHNVLNALAAIAVANELGIADAAIIKALGEFRGVGRRFQRYGDLALPGGGHCTLVD
                     DYGHHPVEMRATLAAARGAFPGRRLLLAFQPHRYTRTRDCFEDFVKVLSGVDALVLTE
                     VYAAGEQPIVAADARALARAVRVAGKVEPVFVEAIADLPQAILDAARDGDVVMTMGAG
                     SIGAIPGKLAAG"
     gene            4341..5273
                     /locus_tag="PLL14_00925"
     CDS             4341..5273
                     /locus_tag="PLL14_00925"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOG02507.1"
                     /translation="MRTMTVKDFGKVAVLFGGRSAEREVSLNSGSRVLAALQRQGVDA
                     TAFDPSERKLDELAAFDRAFIALHGRYGEDGTIQGILELMGIPYTGSGVMASALGMDK
                     WRSKLLWQACGLPVPEYVLLDANSDFARVEAELGLPLFVKPACEGSSIGISKVKQVGG
                     LGVAYAEAAKHDSLVIAERAILGGEYTTAILGDAALPIIKIEPRGEFYDYEAKYLRDD
                     TAYRCPCGLSEERERELRAQALEAFRVLGGRGWGRVDFLMEPAANGSGRAYFLEVNTS
                     PGMTDHSLVPMAARAAGVGYDELVLRVLALAALG"
     gene            5343..6029
                     /locus_tag="PLL14_00930"
     CDS             5343..6029
                     /locus_tag="PLL14_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOG02508.1"
                     /translation="MLVAAVVWGTPRLRLFPLHEVQVTHALQEVRQSDVEQSLSDVLR
                     GNFFTVDIEALRRAVEQLPWVRRAEVWRKWPSRIEVRIEEHRAAAHWGDGPEQLVNTY
                     GELFSAALSQDQQLPRLSGPVGSSSEVLRRYEEFAQILKPTGRLPAQMALSPRLAWLL
                     KLEDGMLVELGREQAKAPIRMRLQRFVDYYPTLSNTRQGRPMAVDMRYPNGFALRFPA
                     SAAQEVKGKK"
     gene            6026..7255
                     /gene="ftsA"
                     /locus_tag="PLL14_00935"
     CDS             6026..7255
                     /gene="ftsA"
                     /locus_tag="PLL14_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOG02509.1"
                     /translation="MSRDSKDLIVGLDIGTSKIVALVAEVTPEGRLNVIGMGSQESKG
                     LKKGVVVNIEETVATISRVLQEVELMADCKVRDVYTGIAGSHIRSFNSNGMVAIKDKE
                     VTTMDVERVIETARAMPIPADQEILHILTQEFIIDDQDGIREPIGMSGFRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVNDLVLQPLASSCAVLSEDEKDLGICLIDIGGGTTDLA
                     VWTQGAIRHTSIIPIAGDQITNDIAMALRTPTREAEDIKRKYGCALAHLADPEDVLDV
                     AGVDDRPSRKLSRRALADVIQPRVEELFELIQAELRRSGFEDVLSSGIVLTGGASVMP
                     GMIELGEEIFHMPVRLGVPKYQGALADVVQSPRFATACGLLLEAQTQRKRGLKVRETR
                     DVKQVFGRMKSWFEKNF"
     gene            7323..8507
                     /gene="ftsZ"
                     /locus_tag="PLL14_00940"
     CDS             7323..8507
                     /gene="ftsZ"
                     /locus_tag="PLL14_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG02510.1"
                     /translation="MFEIIDKDERDSDQDTVIKVIGIGGAGGNAVDHMIREGVNGVID
                     FIAANTDSQALGRSIAVQKLQLGKTGLGAGAKPEAGRSAAIEERDAIAEALRGAHMVF
                     ITAGMGGGTGTGAAPIVAEVARELGVLTVAVVTKPFGFEGKRLKVADVGIAELQKHVD
                     SLIVILNDRLMDVLGDDISMDDAFKAADNVLRNAVGGIAEIINFPGLVNVDFEDVRTV
                     MGEMGMAMMGSANAAGVDRARIAAERAVASPLLEGVNLSGAKGVLVNITATRSLKMKE
                     VNEVMNTVRAFAAEDAHIIFGAVYDQDMGEDIRVTVVATGLGQPQAKRQNFEVINSAS
                     SLNQATGTGDRYPHGPSIDYSSLDQVPAIVRKGRSATVEALANSGVDRYDIPAFLRKQ
                     AD"
     gene            8597..9526
                     /gene="lpxC"
                     /locus_tag="PLL14_00945"
     CDS             8597..9526
                     /gene="lpxC"
                     /locus_tag="PLL14_00945"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOG02511.1"
                     /translation="MLKQRTLKSVVRAAGVGLHSGVKVTISLRPAAPGTGIVFRRIDL
                     DPVVDLRASALLVGDTRMCSCLEHEGAKVGTIEHLMSAFAGLGVDNAFVDLDAAEVPI
                     LDGSASPFVFLIQSVGIEEQPVAKRFIRVKRPIEVRDRDASGEKWARLEPYDGFRLSF
                     SIGFNHPAIDRTGQSVTIDFADHSYIREVARARTFGFMQEVEWLRENGLAQGGGLDNA
                     VVLDEFRVLNADGLRYSDEFVKHKVLDAIGDLYLLGQPLLASFIAHKSGHALNNALAR
                     ELLASPEAWEYVSFENVDLAPQTVSRWLALPAF"
     gene            9531..9719
                     /locus_tag="PLL14_00950"
     CDS             9531..9719
                     /locus_tag="PLL14_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02512.1"
                     /translation="MWLLRLLAVLTMISVAAGVVVFLFTRDARYLQISWRLFRYALIV
                     ALLVFALMILERVAIIPV"
BASE COUNT         1653 a         2950 c         3275 g         1910 t
ORIGIN      
        1 agggcgatgg cgtgatggtc ctcaatcgcg acgatgtgcg cagtctggct gccgctcgtc
       61 cggatcgcga gctggtgacc ttcggccttg atccggcaac gcgcgtcggg gactacggcg
      121 tggtagacgg ctgcatcgtt cgagacggcg agaaactgct tgcccttgac gagttgcgcc
      181 tggtcggccg ccacaatgcc gccaacgcca tggctgcgct ggccttgtgc gaggcggttg
      241 gcatccaggc cggatcggtg ttgccggccc tgttggcgtt cagggggctc gcccaccgcg
      301 tcgagtgggt cgccgaaatc aatggcgtcg gttacttcga cgactcgaaa ggaaccaacg
      361 tgggcgccac cctggcggcc atcgagggtt tgggtcgccg cgttgcgatc atcctcggcg
      421 gcgacggcaa gggacaggat ttctcgccct tgcggcatgc gatcgagcag catgcgcggg
      481 gggtggcgtt gatcggccgc gacgcgtcgg agattgctgc ggtcctggcg ggttgccagg
      541 tgccacaacg cgtttgcgcc gacatggcgg aggcggtgcg ctggtgcgcc gcccaggcac
      601 agcagggcga cgccgtgctg ctctcgccgg cttgcgccag catggacatg tatcgcgact
      661 atgcgcatcg cgccgaagtc ttcgtcgatg ccgttcgtcg tatcgaaagg gaggccgcct
      721 gatgctgcac actctcgacg ccccacgccg tctgccgtcg gaagtcgacc tcgcactgct
      781 gtggagcacc ctgatgctcc tggtgatcgg catggtcatg gtctattcgg cgtcgatggc
      841 cattgccgag gccgggcgcc agaccggcaa ccaatcggcg tattttctga tcaggcatgc
      901 ggtgttcctg gtcgttgcgc tggtcgcctc catggttgcg tttcaggtgc cactggcgac
      961 ctggcagcgc tggtcaccgt ggctgttcgt gggcggattc gtgctgttgg cgctggtgct
     1021 gatacccggt atcggtcgcg atgtgaatgg tgcgcgccgc tggctgcccc ttggaatcgc
     1081 caatctgcag ccctccgaat tgatgaagct gtttgccgtc ctttatgctg ccgactacac
     1141 ggcgcgcaag atgccgcaca tgcacgatct gaaaaaggcc ttcctgccgc tggcaagcgc
     1201 aatggtcgcc gtcggcatcc tgttgctcaa ggaaccggac tttggcgctt tcgtggtgat
     1261 catctcgatc gccatgggca tcctttttct cggcggcatg aaggcgcgcc tgttcgtcgt
     1321 gctgatcatc gtgctgggtg ccgctttcgc cgcgctgatc atcttttctc cttaccggcg
     1381 tgaccgcatt tttggtttca tggacccgtg ggcggacgcg ttcgggcggg gctatcagct
     1441 ttcgcatgcg ctgatcgctt tcgggcgtgg cgaattgctg ggagtcggcc tgggtgcgag
     1501 cgtcgagaag ctgttctatc tgccggaagc ccataccgac tttctgctgg cggtgatcgc
     1561 cgaagaactg ggttttgtcg gcgtcctggt ggtcatcctc ctgttcgggc tgctgatcca
     1621 gcgcgccttc gccatcgggc gccaggccgt cgccctggag cgtctgtact ccgcgctggt
     1681 ggcgcagggc atcggggtct ggctgggcgt gcaggccttc atcaacatgg gcgtgaacat
     1741 gggtctgctg ccgaccaagg gattgaccct gcccttgatg agcttcggcg gttcggggat
     1801 tctcgccaac tgcgtggcgc tggcggtgct gctgcgaatc gactgggaga accgccagtt
     1861 gatgcgtggg gccaaggtat gagcaaggtc ggggcatgaa caggaccctg ctggtgatgg
     1921 ccggcggcac cggcggccac gtatttccgg gtctggcggt ggctgacctg ctgaaaagcc
     1981 gtgactggcg agtcgtctgg atgggtgcgc cggacggttt ggaagcaaag ctcgttccgc
     2041 cgcgcggcta cgaaatggcc tgggtgcgct tcgccgcgtt gcgcggcaag ggtctgctgc
     2101 gcaagctgct gttgcccttt cacctgttcg ccgcgtgctg gcaagcgcgg cgagaaattc
     2161 gccgcgtcag acccgacgtg gttctgggca tgggtggcta cgtcagcttt cccggcggca
     2221 tgatggcggc gctgctgggc tgcccactga tcatccatga acagaattcg attgccgggc
     2281 tggccaaccg ggtgctcgca cgctttgcca ggcgcgtcgc ctgcggtttc ccgaatgcgt
     2341 tgccgagcgg cgtgtgggtc ggcaatcccc tgcgtccgga aatgagccgc gtgccggcac
     2401 cggcgcagcg tctcgccggc cgtcaggcgc cgctgcacct gctggtcctc ggcggcagtc
     2461 agggcgccgc ggcgctcaat gacatcgtgc cccgggggct ggccttgatc gattccgagc
     2521 agcggccgtt ggtcgtgcac caggcgggcg agaagcacct cgcgcagctt cgccagaact
     2581 acgaggcggc cggtgtgcag gcgcactgcg cggcgttcat cgacgacatg gccggggcct
     2641 acgagtgggc tgacctggtg atctgccgcg ccggcgcgct caccgtcgcc gaactggcgg
     2701 ccactggcgt ggccagcgtg ctggtgccgt ttccgtacgc cgtggacgac catcagacgc
     2761 acaacgccat cttcctgtcg caggcgggtg gcgccatcct gttgccgcag gaccagatga
     2821 cgcccgaatc gatcagcgag atccgcaact atccccgtag ccagttgcag gaaatggccg
     2881 aaaaatccca tgaactggcc agaccggaag cgacggcgga gcttgcccgg atgtgcgagg
     2941 agcttgcgca gtgaaacaca aagtcaagaa catccacttc gtcggcatcg gcggcgcggg
     3001 catgagcggc atcgccgaag tgctggccaa tctgggcttt tcggtcagcg gctcggacct
     3061 tgccgagagt tcgacgaccc ggcgcctggg cagcctcggc gtacggatca tgatcgggca
     3121 tgccgccgcg aacgtggacg ccgccgaggc ggtggtagtt tcttcagcgg tcaagtccga
     3181 caaccctgaa gtccttgccg cgcgtcgtcg caaggtgccg gtggtgccgc gtgcgcagat
     3241 gcttgccgag ttgatgcgcc tcaaacaggg catcgccgtc gccggcacgc acggcaagac
     3301 caccaccacc agtctggtgg cgtcgattct tgccgagggt ggcatggacc cgaccttcgt
     3361 cattggtggc cggctcaacg ccgccggcgc caatgcacgc ctggggtccg gcgattttct
     3421 ggtggccgag gccgacgagt ccgatgcttc cttcctgctc ctgtcgccgg tgatttcgat
     3481 cgtcaccaac atcgatgccg atcacatgga gacctatggc catgatttca gccggctcaa
     3541 gcaggcgttc gtcgatttcc tgcagcgcct gccgttctat ggcgtggccg tgctctgcgc
     3601 tgacgacgtc aacgttcgcg aaatcatgcc cctggtcagc aagcagatcg tcagctacgg
     3661 tttcgacgcg tcggcgaaca tctatgccga gaacctgacc gccgtcgccg gccagatgca
     3721 tttcgattgc gttcgggtca acgggagcgt cagtcgactg ccggtgatcc tcaacctgcc
     3781 cggtcgacac aatgttctga acgcgctcgc agcgatcgcc gtggccaacg aactgggcat
     3841 tgccgacgcg gcgatcatca aggcattggg cgaatttcgc ggtgtcggcc ggcgttttca
     3901 gcgttacgga gatctggcgt tgccgggcgg cggccactgc acgctggtcg acgactatgg
     3961 gcaccatcct gtcgagatgc gcgcgaccct ggccgctgcg cgcggggctt tcccgggtcg
     4021 ccgtctgctg ctcgccttcc agccgcaccg ttacacccgt acgcgtgact gtttcgagga
     4081 tttcgtcaag gtcttgagcg gcgtcgacgc gctggtgctg accgaagtct atgcggccgg
     4141 cgagcagccc atcgtcgctg ccgacgcgcg cgcactggcg cgggcggtac gggttgccgg
     4201 caaggtcgag ccggtattcg tcgaagccat tgccgacctg ccgcaagcga ttctggacgc
     4261 cgcccgcgat ggcgacgtgg tgatgaccat gggcgccggg tcgatcggcg cgattcccgg
     4321 caaactggct gccggctagg atgaggacga tgacggtcaa ggatttcggc aaggtcgcgg
     4381 tgctcttcgg ggggcgttcg gccgagcgtg aagtgtcgct gaacagtggt tcgcgggttc
     4441 tggcggcttt gcagcggcag ggcgtggatg ccactgcctt tgatccgtca gagcgcaagc
     4501 tcgacgaact ggcagccttc gatcgcgctt tcattgcgct gcatgggcgt tacggcgaag
     4561 acggcaccat tcagggaatc cttgaactga tgggcattcc ctataccggt agtggcgtga
     4621 tggcttcggc actgggcatg gacaaatggc gcagcaaatt gctctggcag gcttgtgggc
     4681 taccggtacc ggaatacgtg ctgctcgacg ccaacagtga ttttgcccgc gtcgaagccg
     4741 agctgggctt gccgctgttc gtcaagccgg cctgcgaagg ctcgtcgatc ggcatcagca
     4801 aggtcaagca ggtgggcggt cttggcgtgg cctacgccga agcggcgaag cacgattcgc
     4861 tggtgattgc cgaacgcgcg atcctgggcg gcgaatacac gaccgccatt ctcggcgatg
     4921 cggcgctgcc gatcatcaag atcgaaccgc gcggcgagtt ctacgattac gaagccaaat
     4981 acctgcgcga cgacaccgct taccgctgtc cctgcggcct gtcggaggaa cgcgagcgtg
     5041 aattgcgcgc gcaggccctt gaggccttcc gggtcctcgg tggccgcggc tgggggcggg
     5101 tcgacttcct gatggagccg gcggcgaacg gcagcggtcg ggcctatttt ctggaggtga
     5161 atacctcgcc cggaatgacc gatcactcgc tggttccgat ggcggcacgc gccgccggcg
     5221 tgggctatga cgaactcgtg ctgcgggtgc tggcactcgc ggcactggga tgagcgatgt
     5281 ggcacaaacc gcgggtgatg acggctgttt ccgacctgct cttcctggca ggtgccgcgg
     5341 cgttgctggt cgcggcggtc gtttggggga cgccgcggct gcgcctgttt ccgctgcacg
     5401 aagtacaggt gacgcacgcc ttgcaggagg tgcgccagag cgacgtggag caatcgctgt
     5461 cggacgtgct gcgcggcaat tttttcaccg tcgatatcga agcattgcgc cgcgccgtcg
     5521 agcagttgcc gtgggtgcgg cgtgccgagg tctggcgaaa atggccgtca cgcatcgagg
     5581 tgcggatcga ggaacatcgg gccgcagcgc actggggcga tgggccggag caattggtca
     5641 acacctacgg cgagctgttt tccgccgcgc tgtcgcaaga tcagcagctt ccccggctca
     5701 gcggaccggt cggttcgtcg agcgaagttc tgcgccgata cgaggagttc gcgcagatcc
     5761 tgaaacccac cggccggctg ccggcgcaga tggcgttgtc gcctcgcctg gcctggctcc
     5821 tgaaactgga ggatggaatg ttggttgaac tgggtcgcga gcaggccaag gcgccgatcc
     5881 gcatgcgcct gcagcggttt gtcgactact acccgacact gtcgaacacc cgccaggggc
     5941 ggccgatggc ggtggatatg cggtatccaa atggtttcgc attgcgcttt ccggccagtg
     6001 cggctcaaga agtcaaagga aagaaatgag cagggatagc aaggatctga tcgtcggact
     6061 ggacatcggc acgtcgaaga ttgtggcact ggttgccgaa gtcacgccgg aggggcggct
     6121 gaacgtcatc ggcatggggt cgcaggagtc gaagggtctc aagaaaggcg tcgtggtcaa
     6181 cattgaagag actgtcgcca caatctcccg cgtgctgcag gaagtggaac tgatggccga
     6241 ttgcaaggtg cgagatgtgt acaccggaat cgccggcagt cacatccgca gcttcaactc
     6301 caacggcatg gtggccatca aggacaagga agtcaccacc atggatgtcg agcgggtgat
     6361 cgaaacggcg cgggcgatgc cgattcccgc cgatcaggaa atcctgcaca tcctgaccca
     6421 ggaattcatc atcgacgacc aggatggcat ccgcgagccg atcggcatga gtggtttccg
     6481 gctcgaggtg aaggtgcaca tcgtcaccgg agcggtttcc gccgcccaga acatcgtcaa
     6541 gtgcgttcgc cgttgcggac tggaggtgaa cgacctggtg ctgcagccgc tggcgtcgag
     6601 ttgcgcggtg ctctcggaag acgagaagga cctcggcatc tgcctgatcg acattggtgg
     6661 cggcaccacc gatctggcgg tgtggacgca gggggccatc cgccacacgt cgatcattcc
     6721 catcgccggc gaccagatca ccaatgacat tgcaatggca ctgcgtacgc cgacgcgcga
     6781 ggccgaagac atcaagcgca agtatggctg tgcgctggcg catctggcgg acccggaaga
     6841 cgtgctggat gtcgccgggg tagacgatcg tccttcgcgc aaactgtcgc gccgggcgct
     6901 ggcggatgtc atccagccgc gcgtcgagga attgttcgaa ctgatccagg cggaacttcg
     6961 tcgttccggt ttcgaggatg tgttgtcctc cggcatcgtc ctgaccggag gcgcctcggt
     7021 catgccgggg atgatcgaac tgggcgagga aatctttcac atgcccgttc gcctgggtgt
     7081 tcccaagtat cagggggcac ttgccgatgt ggtgcagagt ccgcgttttg cgacggcctg
     7141 cggtttgctg ctggaagcac agacgcaacg caagcgcggc ttgaaggtgc gggaaacacg
     7201 cgacgtcaaa caggtgttcg ggcgcatgaa atcctggttt gaaaagaatt tctgatcccg
     7261 ttgtgaacga taatcacacg ttttttacca gctattgagc tacttttgca gaggaggcaa
     7321 caatgtttga aatcatcgac aaagacgaaa gagacagcga tcaggatacg gtgatcaagg
     7381 tcatcgggat cggcggcgcc ggaggcaatg ccgtcgatca catgatccgc gaaggagtca
     7441 atggtgtgat cgacttcatc gccgccaata ccgactcgca ggcgctgggc cgcagtattg
     7501 ccgtccagaa actgcaactg ggcaagaccg gccttggcgc cggtgccaaa ccggaagccg
     7561 ggcgcagcgc cgcgattgag gagcgcgacg ccatcgccga ggcgctcagg ggtgcacaca
     7621 tggtgttcat caccgccggc atggggggcg gcacgggaac cggcgccgcc ccgatcgttg
     7681 ccgaggtggc ccgcgaactc ggcgtgctga ccgtggccgt ggtgaccaag ccattcggtt
     7741 tcgaaggcaa gcgcctgaaa gtggcagacg tcggcattgc cgaacttcag aagcacgtcg
     7801 attcgctgat cgtcatcctg aatgaccggc tgatggacgt cctcggcgac gacatttcga
     7861 tggatgatgc cttcaaggcc gccgacaacg tgttgcgcaa cgcggttggc ggaattgccg
     7921 agatcatcaa cttccccggc ctggtcaacg tcgacttcga ggacgtgcgt acggtgatgg
     7981 gcgaaatggg catggccatg atgggctcgg ccaacgccgc cggtgtcgac cgcgcgcgca
     8041 ttgccgccga acgtgcggtg gcctcgccat tgctcgaagg cgtcaacctg tccggcgcca
     8101 agggcgtact ggtcaacatc accgccacgc gctccctgaa gatgaaggaa gtgaatgagg
     8161 tgatgaatac cgtccgcgcc ttcgcggctg aagacgcgca tatcatcttc ggcgccgtct
     8221 atgatcagga catgggcgag gacatccgcg tcacggtggt ggccacgggt ctcggtcagc
     8281 cgcaggccaa gcggcagaat ttcgaagtga tcaactcggc ttccagcctc aatcaggcca
     8341 ccggcaccgg cgaccgttac ccgcatgggc cgagcatcga ctactcaagc ctggatcagg
     8401 ttccggcaat cgtgcgcaag ggtcgcagcg cgaccgtcga agcgctggcc aatagcggcg
     8461 ttgatcgcta cgacatcccg gcgttcctga gaaaacaggc cgactaggcc gggccgccgc
     8521 ctactcctct gcaggcggat tccggggcag gccagcctgt gctagaatcc gccgattcct
     8581 gcggtttttc agatccatgt taaagcagcg aacactcaaa tcggtggtcc gcgccgctgg
     8641 cgtcggacta cattccggcg tcaaggtgac catcagcctg cgcccggcag ctcccggtac
     8701 cggcatcgtt tttcgccgca ttgacctcga tccggtggtc gacctgcgcg cttcggccct
     8761 gctcgtcggc gatacgcgca tgtgctcgtg cctggagcac gagggcgcca aggtgggcac
     8821 gatcgaacac ctgatgtcgg cttttgccgg cctcggcgtc gacaacgcct tcgtcgacct
     8881 ggacgccgcc gaagtgccga ttctggacgg atcggcgtcg ccattcgtct ttctgattca
     8941 atcggtcggc atcgaagagc aaccggtcgc caaacgcttc atccgcgtca aacgcccgat
     9001 cgaagtccgc gaccgcgacg cctccggcga aaagtgggcg cggctcgagc cttacgacgg
     9061 attccgcctg tcgttttcga ttggtttcaa tcatccggca atcgatcgta ccggtcagtc
     9121 ggtgaccatt gattttgccg accactcgta tatccgtgaa gtcgcgcgcg cgcgcacctt
     9181 cggtttcatg caggaagtgg aatggttgcg cgagaacggt ctggcgcagg ggggcggcct
     9241 ggacaacgcc gtcgtgctcg acgaatttcg cgtgctcaac gccgacggtc tgcgctatag
     9301 cgacgagttc gtcaaacaca aggtgctcga cgcgatcggc gacctctacc tgctcgggca
     9361 gcccttgctg gcctcgttca tcgcccacaa atcggggcat gcgctgaaca atgcgctggc
     9421 ccgcgaactg cttgccagcc ccgaagcctg ggaatacgtc agtttcgaaa acgtcgacct
     9481 ggcaccacag acggtttccc gctggctggc gttgccggcg ttctgacggc atgtggttgt
     9541 tgcgcctgct ggcggtcctg acaatgatca gcgtcgccgc cggcgtggtg gttttcctgt
     9601 tcaccaggga cgcacgctac ctgcagattt cctggcgtct gtttcgctat gcgttgatcg
     9661 tcgccctgct cgtcttcgcg ctgatgatcc ttgagcgggt ggcgatcatt cctgtctaga
     9721 cgaacgcgcc agcagcgttt cgagtgcagc acgcagcggt gatgccggca gcgattcgct
     9781 caagtgct
//
LOCUS       DAOSIT010000017         9744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6736_length_9744_cov_10.493756, whole genome shotgun sequence.
ACCESSION   DAOSIT010000017 DAOSIT010000000
VERSION     DAOSIT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_9744_cov_10.493756"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>2186
                     /locus_tag="PLL14_00955"
     CDS             46..>2186
                     /locus_tag="PLL14_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG02513.1"
                     /translation="MSFYIALILAAFTIVFGTRHLDATERHEGMVAAIALESGVKLVA
                     FLAVGGFVTYGLYDGFGDIFEHITTNPELRHLATAIPSGAGYTGWFSLIVLSGMAVLF
                     LPRQFQVAVVENVNEAHLKRAAWLFPLYLLLINIFVLPIALGGLLHFAGQGVDADTFV
                     LTLPMSQRQEGLALLVFIGGLSAATGMVIVETIALSTMVCNDLVMPLLLHHKRLAIDE
                     AKDLSGVLLRIRRGAIVLILLLGYLYFRLAGEAYALVAIGLISFAAVAQFAPAMIGGM
                     YWRGGTRGGALAGLAAGFLVWAYTLLLPSFAKSGWLPDEFLMLGLFGVELLRPQQLFG
                     LSGFDEISHCLFWSFFANIGAYVGVSLLRPPMVAEATQATVFVDALKNNGPVGAALWR
                     GRAKVSDLQELVGRFLGPTRAQTLFVAYARRRGHADGVMLEADAQLVQFAESLLAGAI
                     GSASARVMVASVAKEEPLSIEEVMHILDEASQLRTYSRELERKSRELTAATLELQEAN
                     ERLQELDRVKDDIMSSVTHELRTPLTSIRAFSELLRDDPRMHLADRERFLGLIVSEAE
                     RLTRLINQTLDLAKIESGRADWNGCELDLKEVIEQSIAATSQLFREKGADVELDLPDN
                     PPLILADRDRLIQVMLNLLSNAVKFLAPGSGRVRVVLQRLADGLEVSVADNGPGIRVE
                     DQQLIFEKFRQVGDTMTAKPAGTGLGLPISRR"
     assembly_gap    2187..2286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2289..4208
                     /locus_tag="PLL14_00960"
     CDS             2289..4208
                     /locus_tag="PLL14_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG02514.1"
                     /translation="MGEYGAEELRVLVQAANQVLAVRDALEMDVAERIRQARASLEEE
                     RSRLAALMADLSQSVVVCNLDGRVLLYNQRARQELSSGTDGANAELLGLGRSIYTVFD
                     RALIEHALERLQQASREGAEAADSRRLLGSAQFVTATRAGRLLRAHMSPVLGGEGPHH
                     ARVGAVSITGFVLVLEDVTSTNERHARRDALIQSLIEGGRGPLGSIRAAGEMLSDFSD
                     MPGQQRDRFLGVIRDEARALSAQFENAAAAFSNDLRERWLLEEILGAELVAVAARRIG
                     ERRASERRVLAVKSENIDEDLWLRVDSFGLLQALRYLALRLQDEYEVREVRLSLTRSG
                     PLAQLDLSWLGTFMNNETAMSWLQDPMTSGGEISPLSVVDVIERCNGDIWFQRERVSH
                     RAFFRTMLPAAEAPKDLPHAIMAAPEGERPEFYDFDIFLWSAASHELDDRPLVELSYT
                     VFDTETTGLLPSEGDEIIQIGATRIVNRRLLRHECLDQLVDPRRPLSPQSIAIHGITP
                     DLLVGQPPIEIVLPKFHAFCADTVLVGHNAAFDMRFLQLKEEQTGVRFEQPVLDTFLL
                     SAVIHPNQTSHRLEAIAERLGVSVFGRHTALGDAMVTGEVFLKMLPLLAEMGITTLRQ
                     AREASQRTFHARLKY"
     gene            4189..>4529
                     /locus_tag="PLL14_00965"
     CDS             4189..>4529
                     /locus_tag="PLL14_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOG02515.1"
                     /translation="MPDSNTESATRAASFSPYVALRSLVRGRALTVGPAVSVREALLL
                     LDRMRADAAVVLDEGTRVPLGIMTLPDVVRRVAIEGCDLQAPIAAVMTSGLITVPADG
                     TVHQASVVMVRR"
     assembly_gap    4530..4629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4630..5529
                     /locus_tag="PLL14_00970"
     CDS             <4630..5529
                     /locus_tag="PLL14_00970"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOG02516.1"
                     /translation="LSSLNDLLTLQVIELVAGQFDLPYVPWCWLVFGSEGRLEQTLAT
                     DQDNGLIFAAESDAQASSLRTAFLPFARAVNEALDACGFPLCKGNIMASNPQWCLSTS
                     EWRSAFSGWLELPQPEALLNSSIFFDFRALYGQESLVNELRAWLLSRASSYPIFLRAM
                     VENTLNWESPLNWWNGFRYDRDKAFPHTIDLKKHGSRPFVDAARIHALASRIPDTNTV
                     ERLRVSGEKMGMAAEELAALVDAFHHIQRLRLEHQMRGGAGSDCNRIDPDDLHELDRL
                     ILKESLKQVQNLQRRLARAHAPV"
     gene            complement(5583..6008)
                     /locus_tag="PLL14_00975"
     CDS             complement(5583..6008)
                     /locus_tag="PLL14_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG02517.1"
                     /translation="MPRIQIDLPENFAFSTEITLYLSHMNYAGHLDNAALLAVVSETR
                     ARFFQSLGYTELDVEGLGIVIADAALQYKSEAFHGEVMVVQMTARDFGSKGCDLLWCM
                     REKSSQREVARGKTGIVFYDYGVRKVAAVPENFRERAAS"
     gene            complement(6167..6346)
                     /locus_tag="PLL14_00980"
     CDS             complement(6167..6346)
                     /locus_tag="PLL14_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02518.1"
                     /translation="MNTDLKREEIMMIVERARYQRSIAAGEVTAVSLRKSLAWLAKWT
                     DRFLHALLMSPTANH"
     gene            6462..>7348
                     /locus_tag="PLL14_00985"
     CDS             6462..>7348
                     /locus_tag="PLL14_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOG02519.1"
                     /translation="MTYRLPPLNALRAFEAAARHLSFKDAGEELSVTPTAISHQIRGL
                     EEYLGFYLFRRLTRALELTREGRAMLPKVREGLECFAAAIESTRQHDAGGRLLVTSPP
                     AFANRWLIRHLPAFTAAYPEIQLHMAASLDTIDARDVSGVSGLEGIDVRDDEAEVFIR
                     FGHGQYDGCRVDRLFAPAYAVVCSPRLLLGMRPLNEPGDLRRHNLLHDESIMELLDRP
                     TWAEWLQVAGVSGINPDAGTHFSDPGLVLSAAIDGVGVAIASKPLIEAEVTAGRLVVP
                     FDIVIRRTQAYYLVAPEAIA"
     assembly_gap    7349..7448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7449..7923)
                     /locus_tag="PLL14_00990"
     CDS             complement(<7449..7923)
                     /locus_tag="PLL14_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOG02520.1"
                     /translation="MSKTLTALVDRVGNEQLLTKPLESARKFWLFGLGAYSLACKSGA
                     RTVETLVQQGKAFEPKARQQIAEKSAELLSSASGKFQRGEELVKERLIRPLDFLVVAS
                     KRDVELLTMRVIQLSAEVRSLAGGKTKPIAKPLSKPSSKPAARVAAKPEAKPAAAD"
     gene            8232..9653
                     /locus_tag="PLL14_00995"
     CDS             8232..9653
                     /locus_tag="PLL14_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOG02521.1"
                     /translation="MHKLSLLDVAFFIAESEASPKHVGGLMICKRPPRSKPGFAADLY
                     REFLTFTDVQAPFNRLIHFSLTTMPTWRDCESVDLQQHVFYHKLPRGKNGRPELYRLV
                     ADLHQPMLDRSRPLWEMHVIDGLSEGRFAVYVKTHHASADGVTMMRWAVDSLSPRADD
                     LELKPIWSIGHALGHRSEKRLNEKMAQSLLANLAGAGKRVLGIGRLAAMLLLESIKLT
                     KNAISLPFVADGNTPLTGQVTAGRQFATASVSMDRVSLIRTRTRSTLNHVALTCLDGA
                     LHRYLRDEGVDLQRPITIQMPVNLRKEGDQGAGNKIGIIQVELSPPTDDPYVRLRNIG
                     FSLRNVRTMIDSVAPEAIESYTLITGVVGQLAEMLKLSNHLPPTGNTLVSNVPGPREY
                     LYIKGARLEELHPISTLPAGNLLNITLFSYAGQLFFGLIATDELPNLERLSGYVAEAF
                     TELEQSVLDAHQPPIPGREPRET"
BASE COUNT         1649 a         2840 c         3011 g         1944 t
ORIGIN      
        1 atcgtgatgc cggaccgcag tgcgacgatg tcggtggcgg ccgacatgag cttctacatc
       61 gccctgatcc tggcggcgtt cacgattgtc ttcggaaccc ggcacctcga tgccaccgaa
      121 cgccatgaag gcatggtggc ggcgattgcg ctggaatccg gagtcaaact ggtggccttc
      181 ctggccgtcg gcgggttcgt tacctacggc ctttacgatg ggttcggcga catcttcgag
      241 cacatcacca caaaccccga gctgcgccat ctggcgaccg cgattccgtc gggcgccggc
      301 tataccggct ggttttcgct gatcgtgctt tccggcatgg cggttctctt cctgccgcgc
      361 cagttccagg tcgcggtggt cgaaaatgtc aacgaggcgc acctgaagcg tgccgcctgg
      421 ctgtttccac tgtatttgct gctgatcaat atcttcgtgc tgccgatcgc gcttggcggg
      481 ctgctgcatt ttgccgggca gggggtcgac gccgatacct tcgtcctgac cctgccgatg
      541 tcgcagcgcc aggaggggct ggcgctgctg gtattcatcg gtggcctgtc ggcggcgacc
      601 ggcatggtga tcgtcgaaac cattgccctg tcgacgatgg tctgcaacga tctggtgatg
      661 cctttgctgt tgcatcacaa gcgattggcg atcgacgagg cgaaggatct ctccggcgtg
      721 ttgctccgca tccggcgcgg tgcgatcgtc ctgatcctgc tgctgggcta cctctacttc
      781 aggcttgccg gcgaagctta cgccctggtg gccatcgggt tgatcagttt cgccgcggtg
      841 gcccagtttg ccccggcgat gatcggcggc atgtactggc gcggcggaac gcgcggcggt
      901 gccctggccg gcctggcggc aggtttcctg gtctgggcct ataccttgct gctgccgtcg
      961 tttgccaagt cgggctggct tccggacgag ttcctgatgc tcggcctgtt cggagtcgaa
     1021 ctcttgcgac cccagcaatt gtttggtctc agtggcttcg acgaaatttc ccactgcctg
     1081 ttctggagtt tcttcgccaa tatcggcgcg tatgtcgggg tctcgctgct gcggccgcca
     1141 atggtcgccg aggcgaccca ggcgacggtt ttcgtagacg cgctgaagaa taacggtccg
     1201 gtaggggccg ccttgtggcg cgggcgagcg aaagtgagcg acctgcagga actggtcggg
     1261 cgtttcctcg gcccgacacg ggcacaaacg ctgtttgtgg cctacgcacg ccggcgtggt
     1321 catgccgacg gcgtgatgct cgaagccgat gcgcagttgg tgcagttcgc cgaatcgctg
     1381 ctggcgggtg ccattggcag tgcgtcggcg cgggtcatgg tggcctcggt ggccaaggag
     1441 gagccgctga gtatcgaaga ggtcatgcac attctcgacg aagcatcgca gttgcgcacc
     1501 tacagccgcg aactggagcg caaatcgcgc gagttgacgg cggccacgct ggagttgcag
     1561 gaagccaacg agcgcctgca ggaactcgat cgcgtcaagg acgacatcat gtcctcggtg
     1621 acgcacgaac tgcgtacgcc gctgacatcg atccgcgcct tttcggaatt gctgcgcgac
     1681 gaccccagga tgcacctggc ggatcgcgaa cgttttctcg ggctgatcgt cagcgaggcc
     1741 gagcggctga cgcgattgat caatcagaca ctcgatctgg cgaagatcga atcgggacgc
     1801 gccgactgga atggctgtga actcgacctc aaggaggtca tcgagcagtc gattgccgcg
     1861 accagtcaac tgtttcgtga gaaaggagcg gacgtggaac tcgatctgcc cgataatccg
     1921 ccgttgattc tcgcagatcg cgatcgactg attcaggtca tgttgaatct gctttcgaac
     1981 gcggtaaagt tcctggcgcc aggtagcgga cgtgttcgtg tcgtgctgca gcggctggcg
     2041 gatggactcg aagtcagtgt ggccgataat gggcccggca ttcgtgtgga agatcagcaa
     2101 ctgattttcg agaagtttcg ccaggtcggc gatacgatga ccgccaagcc tgcgggtacc
     2161 ggtctcgggc tgccgatcag ccggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnntgct gggcgagtat ggcgcagaag aactgcgagt cctggttcag gccgccaatc
     2341 aggtgctggc ggtgcgcgac gcactggaaa tggatgtcgc cgagcgcata cgccaggcgc
     2401 gcgcttccct tgaggaagag cgcagccgcc tggcggcatt gatggctgac ctcagccaga
     2461 gtgtcgtggt ctgcaacctc gatggccggg tcctccttta taaccagcgg gccaggcagg
     2521 agctttcgag cggtacggat ggcgcgaatg ccgaattgct gggtcttgga cgttcgattt
     2581 acacggtgtt tgaccgggca ctgatcgagc atgcgctcga acgtctgcag caggccagtc
     2641 gcgagggggc ggaggcggcg gatagccgga gactgctcgg atcggcgcag ttcgttacgg
     2701 cgacgcgcgc gggtcgcctg ttgcgcgcgc acatgtcgcc ggtgcttggc ggcgagggtc
     2761 cgcaccacgc ccgcgtgggc gctgtttcga tcaccggatt cgtgctcgtg ctcgaagacg
     2821 tcaccagtac caacgagcgg cacgcgcgcc gcgatgcgct gattcagtcg ctgatcgaag
     2881 gcggtcgcgg gcccctgggc agcattcgtg ccgcaggcga aatgctctcc gatttttccg
     2941 acatgcctgg ccagcaacgc gaccgttttc tgggcgtcat ccgtgacgag gcgcgggcat
     3001 tgtcggcaca gttcgagaac gcggcagcgg ccttctcgaa cgatctgcgc gagcgctggt
     3061 tgctggaaga gatcctgggt gcagaactcg tcgcggtcgc cgcccggcgc atcggcgagc
     3121 ggcgtgccag cgagcgtcgc gtactggcgg tgaagagcga gaatatcgat gaagacctgt
     3181 ggctgcgggt ggacagcttc ggacttctgc aagccttgcg ctatctggcc ctgcgcttgc
     3241 aggacgagta cgaggtgcgc gaggtgcgtc tgtcgctgac gcgcagcggg ccattggcgc
     3301 agctcgatct gtcatggctg ggcacgttca tgaacaacga gacggcgatg agctggttgc
     3361 aggatccgat gaccagcgga ggcgaaatat ctccgctgag cgtcgtcgat gtgattgagc
     3421 gctgtaacgg cgacatctgg tttcagcgcg agcgggtgtc gcatcgcgct ttctttcgga
     3481 cgatgttgcc ggccgccgag gcgccgaagg atctgccgca tgcgatcatg gcggcaccgg
     3541 agggcgaacg tcccgagttc tatgatttcg atatcttcct gtggtcggcc gcgagccacg
     3601 aactcgacga ccggcctctc gtggaactgt cctacaccgt attcgatacc gaaacgaccg
     3661 gcctgttgcc gtccgaaggc gacgaaatca tccagatcgg cgcgacacgc atcgtcaatc
     3721 gtcgactgct gcgccatgag tgtctcgatc aactcgtaga cccgcgccgt ccactttcgc
     3781 cgcagtcaat cgccatccac ggcatcaccc cggatctgct ggtcgggcag ccgccgatcg
     3841 agattgtcct gccgaagttc catgcctttt gcgctgacac ggttctggtc gggcacaacg
     3901 ccgctttcga catgcggttc ctgcaattga aggaagaaca gaccggggtg cgtttcgagc
     3961 aaccggtgct cgacaccttc ctgctttcgg ccgtaatcca tcccaaccag accagccatc
     4021 gcctggaagc gatcgccgaa cgcctcgggg tatcggtttt cgggcgccac acggcgcttg
     4081 gcgatgcgat ggtgactggc gaagtgttcc tgaagatgct gcccctgctc gccgagatgg
     4141 gcatcacgac cttgcggcag gcacgtgaag ccagtcagag aactttccat gcccgactca
     4201 aatactgagt cggcaacacg ggcggcatcg ttctcgccgt atgtcgcgct gcgcagtctg
     4261 gtgcgcgggc gagcgctcac ggtcggtcca gcggtcagcg tgcgcgaggc gctgctgctg
     4321 ctcgaccgca tgcgggctga cgccgcggtg gtgctcgatg agggtacccg ggtgccgctg
     4381 ggcatcatga ccctgcccga tgtggtgcgc cgcgttgcca ttgagggctg cgacctgcag
     4441 gcaccgatcg ccgcggtgat gaccagtggc ctgattaccg tgcctgctga cggcaccgtt
     4501 catcaggcca gcgtggtgat ggtcaggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnc tttcgtcgct caacgatttg ctgacgctgc aggtcatcga actggttgcc
     4681 ggccagttcg atctccctta cgtaccctgg tgctggctgg tgtttggctc ggaaggcagg
     4741 cttgagcaga cgctggccac cgaccaggac aacgggctga tctttgctgc tgaatcggat
     4801 gcgcaggcga gcagcctgcg cacggcgttc ctgccctttg cccgggcggt caatgaggca
     4861 ctcgatgctt gtggtttccc gctgtgcaaa ggcaacatca tggccagcaa cccgcagtgg
     4921 tgtctatcca ccagtgagtg gcgaagtgcc ttttctggct ggctggagtt accacaaccc
     4981 gaggcgctcc tcaattcgag catcttcttc gactttcgcg cgctttatgg gcaggagtcg
     5041 ctggtcaatg aattgcgtgc ctggttgctg tccagagcgt cgtcgtaccc gatcttcctg
     5101 cgcgcgatgg tcgagaacac gctcaactgg gagtcgccgc tcaactggtg gaatggcttc
     5161 cgctatgatc gggacaaggc ttttccgcat accatcgatc tcaagaagca cggctcgcga
     5221 cccttcgtag acgctgcacg catccatgcc ctggcaagcc ggataccgga taccaatacg
     5281 gtcgagcggc tacgcgtcag cggcgagaag atggggatgg cagcggagga gttggcggcg
     5341 ctggtcgacg cctttcatca cattcagcgt ctccggctcg agcaccagat gcgcggcggc
     5401 gccggatccg actgtaaccg aattgatccc gacgatctgc acgaactgga ccgcctgatc
     5461 ctgaaggaat cgttaaagca ggtccagaat ttgcagcggc gcctggcgcg tgcgcatgcg
     5521 ccggtttagg cgacttttcc ggcagttgcc gtagccgttc ggaggggctt gtcgccgggt
     5581 cttcaggatg ctgcgcgctc gcgaaagttt tcgggtacgg cagccacctt gcgtacgccg
     5641 taatcgtaga aaacaatgcc ggtcttgccg cgtgcgacct cgcgttgact cgatttctca
     5701 cgcatgcacc atagcaggtc gcagcccttg ctgccgaaat cccgggccgt catctgtacg
     5761 accatcacct cgccgtgaaa cgcttccgac ttgtactgca gcgccgcgtc cgcaatgacg
     5821 atgcccagtc cttcgacatc cagttcggta tacccgagcg actggaagaa tcgcgcccgg
     5881 gtctcggaaa ccacggccaa cagcgctgcg ttgtcgaggt gaccggcgta attcatgtgg
     5941 ctcagataga gcgtgatttc ggtggaaaag gcaaagttct cgggcaggtc gatctggatt
     6001 ctgggcatgg tctggcctgt atcggaggat tgcagaggaa atgttgctcg cgtatggggt
     6061 tccggaaggc agaaccagcg acgctgctcg attgtcctct cttttgatgc aagcggcggc
     6121 gggccgatcc gttgggggct gccctggacg actcgggtcg gcgttttcag tggttcgcgg
     6181 tgggcgacat cagcagagca tgcaggaacc tgtcggtcca ctttgccagc caggccagcg
     6241 acttgcggag gctgacggca gtgacttcgc cagcggcgat gctgcgctgg taacgtgcgc
     6301 gctcaacgat catcatgatt tcttcgcgtt tgaggtcggt attcatttgc ttcttccttg
     6361 ggttgttctc ggtccccgag agtgcatcgt cactgtattt cctgggagac gtgcgggcaa
     6421 atgatcaatt gtcagccgat gcgtgagaat atctcatcca tatgacttac cgtctgcccc
     6481 ctctcaacgc cctgcgtgcc ttcgaagcgg cggctcgcca cctcagtttc aaggacgcgg
     6541 gcgaggaact gtccgtgacg cccacagcca tcagtcacca gatccggggc cttgaggagt
     6601 atctgggctt ttaccttttc cgccgactga cgcgggcgct ggaactgacg cgcgagggcc
     6661 gggcgatgct gccgaaagtg cgcgagggtc tggagtgctt tgccgcggcg atcgagagca
     6721 cgcgacagca cgatgccggc gggcgcctgc tggtgacctc gccaccagcg ttcgccaacc
     6781 gctggttgat ccgccacctg cctgctttca ctgccgctta tccggagata caactgcaca
     6841 tggcggcgtc gctggacacc atcgatgcgc gcgatgtttc cggcgtatcg ggcctggagg
     6901 ggatcgacgt tcgtgacgac gaagccgagg tattcatccg tttcggccac ggtcagtacg
     6961 acggttgccg cgtcgatcgg ctgttcgctc cggcctatgc cgtggtctgc agtccgcggt
     7021 tgctgttggg gatgcgaccg ctcaacgaac ccggcgacct gcgccgacac aaccttctgc
     7081 atgacgaatc gatcatggag ttgctggatc gcccgacctg ggccgaatgg ctgcaggtgg
     7141 ccggggtcag cggcatcaat ccggacgccg gaacgcattt cagcgatcct ggactggtcc
     7201 tttcggcagc gatcgacggc gtgggggtgg cgatcgcctc gaagccgctg atcgaagccg
     7261 aggtcacggc cggacgactg gtggtgcctt tcgacatcgt catccgccgc acacaggcct
     7321 actacctggt agcgccggaa gccattgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnca tcagccgccg ccggtttcgc ctccggcttg gctgccaccc tggccgccgg
     7501 cttgctcgat ggtttgctca atggcttggc gatcggtttg gtcttgcctc cggccaggct
     7561 acgcacttcg gcactcagtt gaatcacgcg catggtgagc agttcgacat cgcgcttgct
     7621 ggccacgaca aggaaatcca gtggccggat gaggcgttcc ttgaccagtt cctctccgcg
     7681 ctggaatttg ccgctggcgc tgctcagcaa ctcggccgat ttctccgcga tctgctggcg
     7741 cgccttgggt tcgaaggcct tgccctgttg taccagcgtt tcgacagtcc gcgcaccgct
     7801 cttgcaggcc agcgaatagg cgccaaggcc aaaaagccag aatttgcgtg cagattccag
     7861 cggttttgtc agcaactgct cgttgccaac ccgatcgacc aacgctgtga gtgtcttgct
     7921 catttgtttc tcctttatcc gactgaatct gaaaggtctt gcggctgctt gagcagccac
     7981 cgaaagcaag tctatcgaca agctctagag gacacacact aaaagcctga gtgttttcct
     8041 ctagaacgtc gctcgcgcga tgcggtatcc ttacgacact gtggcagcag agcctgcgat
     8101 cgtcggcagt tcgagtggtg gtcgactgcg ccgtgcgtgc cgcacgcccc gacggggtgc
     8161 aaggcagttc ccggcgtggg tgtgcaggcg tccgttggca gcacaacaca cgcaggacga
     8221 aaggaacgcg gatgcacaaa ctctccttgc tggacgtcgc tttcttcatc gccgagtcgg
     8281 aagcgagccc gaagcacgtg ggtggcctga tgatctgcaa gcgcccgccc agatcgaagc
     8341 ctggttttgc ggccgatctg taccgggagt ttctcacctt caccgacgtt caggcgcctt
     8401 tcaaccgctt gattcacttt tcgctgacga ccatgcccac ctggcgggac tgcgagtcgg
     8461 ttgacctgca gcagcacgtg ttctatcaca agctgccgcg cggcaagaac ggacggccgg
     8521 aactgtaccg gctggtagcg gacctgcatc agccgatgct cgaccggtcg aggccgctgt
     8581 gggagatgca tgtcatcgat ggactcagcg aagggcgttt tgcggtatac gtcaagacgc
     8641 atcatgccag cgccgacggc gtgaccatga tgcgctgggc cgtcgattcg ctgtctccgc
     8701 gggccgacga tctggaactg aaaccgatct ggtcgatcgg gcatgcactt ggccaccgca
     8761 gcgagaaacg gctcaacgag aaaatggcgc agtcgctgct ggccaacctg gctggggccg
     8821 gcaagcgtgt gctggggatt gggcgtctgg cggcgatgct tttgctggaa agcatcaaac
     8881 tcaccaagaa tgccatttcg ctgcccttcg tggccgatgg caatacgccc ttgaccggac
     8941 aggtcacggc tggccgccag tttgcgacgg ccagtgtaag catggacagg gtcagcctga
     9001 tacgtaccag gacccggtcg acgctgaacc atgtcgcact gacgtgcctg gacggcgcct
     9061 tgcaccgcta cctgcgcgac gagggcgtcg acctgcagcg gccgatcacg attcaaatgc
     9121 cggtcaacct gcgcaaggag ggcgaccagg gtgccggcaa caagatcggc atcatccagg
     9181 tcgaactttc gccgccgacc gatgatccct atgtccgcct gcgcaacatc ggcttctcgc
     9241 tgcgcaatgt gcgcaccatg atcgacagtg tcgctcccga ggcgatcgag tcctacacgc
     9301 tgattaccgg tgtggtcgga caactcgcgg aaatgctgaa gctcagcaac cacctgccgc
     9361 cgacgggcaa cacgctggtc tcgaacgtgc cgggtccgcg tgaatacctc tatatcaagg
     9421 gcgctcgcct tgaggagctg catccaatca gtaccctgcc ggccggcaac ctgctcaaca
     9481 tcaccttgtt cagctacgcc gggcaactgt tcttcggttt gattgcgact gacgaactgc
     9541 cgaatctcga gcgtttgagc ggctacgtgg ccgaagcgtt taccgaactc gagcagtccg
     9601 tcctcgacgc acaccagccc cctatccctg gtcgggaacc ccgtgaaaca tgagcaaaaa
     9661 aagaccgggc attgcacccg gtctaatgct cggatttcgt ttttccgcag ggttcgaggc
     9721 gaccctgctt gaacttccac tacg
//
LOCUS       DAOSIT010000018         9561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6887_length_9561_cov_7.112455, whole genome shotgun sequence.
ACCESSION   DAOSIT010000018 DAOSIT010000000
VERSION     DAOSIT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6887_length_9561_cov_7.112455"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1841
                     /locus_tag="PLL14_01000"
     CDS             288..1841
                     /locus_tag="PLL14_01000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOG02522.1"
                     /translation="MARSRTASPHDSTSALGFEAKLWLAADKLRSNMDAAEYKHVVLG
                     LIFLKYISDTFEEHRAKLIAGQGDYKGANPEDADEYRAENIFWVPKEARWSHLQANAK
                     QPTIGKIVDDAMVAIERDNPRLKGVLPKDYARPGLDKHRLGELIDLIGTIGLGDRENR
                     AKDILGRVYEYFLTQFASAEGKNGGQFYTPSCVVRCLVEMLAPYKGRIYDPCCGSGGM
                     FVQSEKFVEAHGGKLGDISIYGQESNATTRRLAVMNLALRGIEADFGKEHADTFRADQ
                     HPDLRADYVLANPPFNDSDWFRKDDDVRWQYGVPPKGNANFAWVQHFIHHLAPQGMAG
                     FVLANGALSATGPQGEIKKAVIDADLVDCIVMLPPKLFYTTQAPVSLWFFSRAKTSPQ
                     GNERRRSRETLFIDARKCFVKSDPTHNTLTDEHIELIANTYHAWKSRGQKGYEDVIGF
                     CRSVRTELIAECDYSLVPARHVGINVDPPDLDSTRQALDEALKSITTSLHEMDELAAH
                     VKESVLKAK"
     gene            1838..2503
                     /locus_tag="PLL14_01005"
     CDS             1838..2503
                     /locus_tag="PLL14_01005"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG02523.1"
                     /translation="MSTVKPPHPILGTLPDDWKVEKLIDCCCYLQRGKAPSYVEESEV
                     LALNQKAVRWGRIEDEHLKYHDPEVEIAAKHFIKAGDVVINSTGDITIGRAYLFHEAP
                     KKLFADSHVTIIRTIKTKLRPEFLVNLLATREYQDLIYSLVTGSTGQLELNKSNLEGL
                     PVICPPIEIQDELAQLWEPLYRSMALADAMRTDCEKAIASLFQRDVMDPWRGARWDGK
                     KVK"
     gene            2695..3102
                     /locus_tag="PLL14_01010"
     CDS             2695..3102
                     /locus_tag="PLL14_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02524.1"
                     /translation="MDFEDSLPKACTELDSALLGLGFKYLEQDTEDSERRAYYIRKFS
                     TDSSNVLLKVVLMFDLSISDDPYASYNENHEMVFNRAYIKVYNRQMEVLRKTRFGVEY
                     DEDTAKSMLVDVYPIAPKSLDHLKGLLEILAAT"
     assembly_gap    3228..3327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3556..3708
                     /locus_tag="PLL14_01015"
     CDS             3556..3708
                     /locus_tag="PLL14_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02525.1"
                     /translation="MPLNESTVEEAALGWFSDLHYAVGHGPAMAPGELQAERESFGEV
                     LGERDS"
     gene            3738..5462
                     /locus_tag="PLL14_01020"
     CDS             3738..5462
                     /locus_tag="PLL14_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOG02526.1"
                     /translation="MNVPDNTALLALLARLDTQPAQELESEHLDFKAWTGPKEDLRTA
                     VEYAVCFANAGGGVVVFGVDDRRKGRTAAIHGAKGYDLDVWRRGIFDSTRPNLTVELD
                     ELAVPEGTGKLLVVRIPKGAMPPYGTAQGVFKQRVGKNCMPLDPARFARARVSSGAID
                     WSGEATDLPTSVLDPVELARARAILRRVNPESELLRLSDEPFLVALGAARGGRVTHAG
                     LILFGGEPLLKERCPQHQVHYVHQTSEVAVSRNDSYRLGLLNVLERIEQNFTGPANAE
                     QELEVGLFKLRVPAFPLGVVREAVLNAVTHRDYGDPGEVLIRHGNGELVVTSPGGFLG
                     GITPENILRQEPISRNRTLAEALEKLRLVERAGIGRRRIYLPMLEFGRRPPFYETDGT
                     RVTLRIFGGAFDARMAALVAKWRQEGVEVDLDGLLLLFHLREHSFIDSAKAAEVLQLD
                     RDTVRGVLDGLTLPPTAMLERRGRTKAVTYHLSKVIASDLLGKSVYSAGRGINPLRYA
                     EMVRVFLEDHRMITPKQCRELLHLGESQSARVEISRLLRKWSAADGFLTREGKPPSVV
                     YHLKEGNP"
     gene            5649..8810
                     /locus_tag="PLL14_01025"
     CDS             5649..8810
                     /locus_tag="PLL14_01025"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOG02527.1"
                     /translation="MPLNESTVEEAALGWFSDLNYAVGHGPAMAPGEPQAERDTFGEV
                     VLVDRLRAAIHKLNPEVPADSQEEALRKVLRLATPSLVQSNRAFHTLLRNGIDVEYAR
                     TDGTAKHDKVWLVDFENERANDWLAVNQYTVIEAGHNRRADIVVFVNGLPLGLIELKN
                     AADGEATIWSAYAQLQTYKAEVPSLLHYNALLIVSDGVEARVGSLTANQEWFKVWRTI
                     EGDGDAPKSALELEVLMRGVFERRRFLQLLQHFIVFEDDPDTGAVHKIIAGYHQFHAV
                     NAAIDETVRASGMGVAGQVDEDPGTYWSGKMSGGKPGDRRAGVVWHTQGSGKSFTMLF
                     FAGRVIREAAMQNPTLVVLTDRNDLDDQLFGQFQRCHEILGQTPVQAPSREKLRELLA
                     VASGGVVFTTIQKFMPEKGEKMPCLSQRRNIVVIADEAHRSQYDLIDGLARHLRDALP
                     GASFIGFTGTPIEKTDANTRAVFGDYISIYDIQRAVTDQATVPIYYESRIAKLGLNTS
                     ELPKLDAEFEEITEGEESTRKEKLKTKWAALEALVGDPKRLALVAADLVAHFEKRSEA
                     NNGKAMIVCMSRRICVDLYAALTKLRPEWASAKDDDSEAEKGKACVVKVVMTGSADDG
                     PGWQPHIRNKERRRKLANRFKDSKDPFRIVIVRDMWLTGFDAPCLHTMYADKPMQGHG
                     LMQAIARVNRVFRNKPGGLVVDYLGLAEQLKKALATYTESGGKGDPTYDTRQAIAVML
                     EKYGIACDMLHGFDWAKWTTGTPAEKLGLIPAGQEHILTQDEGKRRWIQNTIELSRAF
                     ALCAASDEATEIRDDVSFFQALQATLSKKTTTTQKTPEQLDAAVRQLVSQAITTEGEV
                     IDVFTAAGLPKPDIGILSEQFLAEVRGLKYKNVAAELLEKLLNDEIKVRGKRNLVQAQ
                     VFSDKLRQALNAYHNRAISTVEFIDELIKMAKQLSQIDQHNEALKLSPEEVAFYDALA
                     ANESAKQVMGDETLRVIATELITQVRKSVTIDWTIRESAQAKIRVLVKRILNRFGYPP
                     DMQDAAVKTVLEQAKLLCAEWAA"
     gene            9351..>9561
                     /locus_tag="PLL14_01030"
     CDS             9351..>9561
                     /locus_tag="PLL14_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOG02528.1"
                     /translation="MHLHALDIAIIVGYFVLVLCLGLWVSRKGTKDLDSYFLGGKSLP
                     WYVLGVSDASGMFDIAGTMWLVYALF"
BASE COUNT         2052 a         2925 c         2774 g         1710 t
ORIGIN      
        1 gctgccgacg cagccagaaa caaaaggaat tgggtgggat cgaacatgct tcgataaacg
       61 gcacacgcgt tgataccgcc caccgccccc aagccaagcc cgcgctccag ctccgacgtc
      121 gcgcaggctt cccgcccgcc gtctttcact cttcaccctt cagtcttcgc aattcagccc
      181 cctttcactt ttcactcttc taagaaaccc gttgccaaat aagtgtaaac acgcgcaccc
      241 tccccaccgc cagttttctg accccagtca tcctttccct tatcggcatg gcccgttctc
      301 gcaccgcctc cccccacgat tcgacttccg ccctcggctt cgaggccaag ctctggctcg
      361 ccgccgacaa gctccgctcg aacatggacg ccgcggagta caagcacgtc gtcctcgggc
      421 ttatcttcct caagtatatc tccgacacct tcgaggagca ccgcgccaag ctcatcgccg
      481 gccaaggcga ctacaagggc gccaaccccg aggacgccga cgagtaccgc gccgaaaaca
      541 tcttctgggt gcccaaggag gcccgctggt cgcacctcca agccaacgcc aagcagccca
      601 ccatcggcaa gatcgtcgac gacgccatgg tcgccatcga gcgcgacaac ccccgcctca
      661 agggcgtgct cccgaaggac tatgcccgcc ccgggctcga caagcaccgc ctcggcgagc
      721 tgatcgacct catcggcacc atcggcctcg gcgaccggga aaaccgcgcc aaggacatac
      781 tcggccgcgt ctacgagtac ttcctcaccc agttcgccag cgccgagggc aagaacggcg
      841 ggcagttcta cacgccgtcc tgcgtcgtgc gctgcctggt cgagatgctc gccccctaca
      901 agggccgcat ctacgacccg tgctgcggct ccggcggcat gttcgtgcag tcggagaagt
      961 tcgtcgaggc ccacggcggc aagctcggcg acatcagcat ctacggtcag gagagcaacg
     1021 ccaccacccg ccggctcgcg gtgatgaacc tcgccctgcg tggcatcgag gccgacttcg
     1081 gcaaggaaca cgccgacacc ttccgcgccg accagcaccc cgacctccgc gccgactacg
     1141 tcctcgccaa cccgcccttc aacgactccg actggttccg caaagacgac gacgtgcgct
     1201 ggcagtacgg cgtgccaccc aagggcaacg ccaacttcgc gtgggtccag catttcatcc
     1261 accacctcgc gccccaaggc atggccggct tcgtcctcgc caatggtgcg ttgtcggcca
     1321 caggaccgca gggagaaatc aagaaagcag tcatcgacgc tgacctagtg gattgcatcg
     1381 tgatgcttcc gccgaagttg ttttacacaa cacaagcgcc ggtatcgctc tggttcttta
     1441 gtcgggctaa aacgtcacct cagggaaatg aacgccgccg cagccgtgag acgctcttca
     1501 tcgatgcgag aaagtgcttt gtgaaatctg atccgacgca caacacgctt accgatgaac
     1561 atatcgagct aattgcgaac acgtatcacg cttggaaaag ccgcggacaa aagggctacg
     1621 aagacgttat tggcttttgc cgaagcgtaa gaaccgaact catcgccgag tgcgactaca
     1681 gcctcgttcc ggctcgccac gtcggaatca atgttgaccc gcccgacctc gactcgactc
     1741 ggcaggcgct ggacgaagca ttgaagtcaa tcactacgag cctccacgaa atggacgaac
     1801 tcgcggcgca tgtgaaggaa tccgtcttga aggccaaatg agcactgtaa aaccaccgca
     1861 cccaattctc ggcactctgc ctgacgactg gaaagtcgag aagctgattg attgctgctg
     1921 ctacctccag cgcggcaaag cgccaagtta cgtggaggag agcgaagtct tggccctgaa
     1981 tcaaaaagca gttcgttggg gccgaattga ggatgagcat ctcaaatatc acgaccccga
     2041 ggttgagatt gcggcgaagc attttatcaa ggccggagac gttgtcatca actcgaccgg
     2101 agacatcacg attggccgtg catacctatt tcacgaggct cccaagaagc tgtttgcgga
     2161 ctctcacgtc accatcatcc gaaccatcaa gaccaagctg cgacctgagt tccttgtgaa
     2221 cttgctggcg acgcgtgaat accaagatct catctattcg cttgttactg gaagcactgg
     2281 gcagctcgaa ttgaacaaat caaaccttga gggtttgccc gtgatttgtc cgccgatcga
     2341 gattcaggac gaacttgcgc agctttggga gccattgtat cgcagtatgg ctttggccga
     2401 cgcgatgagg acggattgcg agaaggcaat cgcatcattg tttcagcgcg acgtgatgga
     2461 tccgtggcga ggtgctcgct gggatggcaa gaaagtgaaa tagccatgat tgattcacac
     2521 caatcccaca ccctcgacac cctgcgcgac acgctgctgt caaagctgct gagcggggac
     2581 tgccagatca ttgtttaacg gttcagcaaa cctcacaaac gcgcaccggt agcgcaacag
     2641 cgcatggatt tagccagctt gtagtcaaaa taaacaacca acaaaattca atatatggac
     2701 tttgaggact cactcccgaa agcatgcacc gaacttgact cagctctctt ggggctggga
     2761 ttcaagtacc ttgaacaaga cacagaggat tctgaaagaa gggcttacta tattcgaaaa
     2821 ttcagcacag attcgagcaa tgtgctgcta aaggtcgttt tgatgtttga tctttcgatt
     2881 tcagatgacc cgtatgcgtc atacaatgaa aaccatgaaa tggtttttaa tcgggcctat
     2941 atcaaggttt acaaccgcca gatggaggtg ctgcgcaaaa cgcgatttgg cgtcgagtat
     3001 gatgaggata ccgccaagag tatgctggtt gatgtctatc cgattgcgcc aaaatcgctc
     3061 gatcatttga agggattgct cgagatttta gccgctacct aaatcctggc agcagtgctg
     3121 aacttgccgc cgacaacgac gtatctgcta aaacaccaag ggattcccga attttagcca
     3181 gcttgtagtc aaaataaaca accaacaaaa ttcaatatat ggactttnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnntga acttgccgcc gacaacgacg tatctgctaa
     3361 aacaccaagg gattcccgaa ttgccccccc catgagcgaa ctaaagacat gctcagacca
     3421 aacgctgtta ccgactcagc gaggtatctt aacaacgatc ttagcattgc actcaaaccc
     3481 ctgatgcaga tttagaaacc gactggttat aaaagtaata aattttatta cataactctt
     3541 agcattcggt cctccatgcc cctcaacgaa tccactgtcg aagaagccgc actcggctgg
     3601 ttctcggacc ttcactatgc cgtgggccac gggccggcga tggcgccagg cgagctgcag
     3661 gccgagcggg agtcgtttgg cgaggtgttg ggagagcgtg actcttgatg taattctggg
     3721 ctgagtatcc gacgcgcatg aacgtacccg acaacaccgc gcttcttgca ctactcgccc
     3781 ggctagacac acagccggcg caggaactcg aatccgagca cctcgacttc aaggcgtgga
     3841 cggggccgaa ggaggatctg cgcacggcgg tcgaatatgc ggtctgcttc gcgaatgcgg
     3901 gaggcggcgt ggtggtgttc ggagttgatg accggcggaa ggggcgcacc gcggcgatcc
     3961 acggcgccaa ggggtacgac ctcgatgtgt ggcgacgggg catattcgac agcacccggc
     4021 ccaatttgac ggttgagctc gatgagctgg cggtgccgga gggcacagga aagctgctgg
     4081 tggtgagaat tcccaagggg gcaatgcctc cctatggcac cgcccaaggc gtcttcaaac
     4141 agcgagtcgg gaagaactgc atgccgctcg atcccgcgag gtttgcacgg gcacgagtgt
     4201 cgtccggggc catcgattgg agcggagagg cgacggacct gccgacgagt gtgctcgatc
     4261 cagtggagct ggcgcgggcg cgggcaattt tgcgacgcgt caatccggag tccgagctgc
     4321 tccgcctgtc agacgaacca tttctggtgg cgctgggcgc ggcgcgtggc gggcgcgtaa
     4381 cccatgcggg cttgatcctc tttggtggcg aaccgctgct gaaggagcgg tgcccacagc
     4441 atcaagtcca ctatgtgcac cagacgtccg aagtggctgt gtcgcgcaac gattcgtacc
     4501 gcctcggatt gctcaacgta ctggagcgga tcgaacagaa cttcaccggg ccggccaacg
     4561 cggagcagga gctggaggtc gggctgttca agttgcgagt gccggcattc ccgctcggcg
     4621 tcgttcgcga agcggtgctg aacgccgtga cgcaccggga ttacggcgat cctggtgagg
     4681 tgctcatccg ccacggcaac ggtgaattag tggtgaccag ccccggcggt tttctgggcg
     4741 gtatcacacc ggagaatatc ctgcggcagg agccgatcag ccgaaaccgc acgttggcgg
     4801 aggcgctgga aaaactgcgc ctggtggagc gagcgggcat cgggcggcgg cgaatctacc
     4861 tgccgatgct cgaatttggc cggcgtcctc cgttctacga aacggatggg acgcgggtga
     4921 ccctgcgaat cttcggcggt gcttttgatg cacgcatggc tgcgttggtt gccaagtggc
     4981 ggcaggaagg cgtcgaggtc gacttggacg gtctgttgct gctcttccat ctgagggaac
     5041 attcgttcat cgattcagca aaggccgccg aggttctcca actagaccgt gacactgtgc
     5101 gtggagtgct cgacggattg acgctgcccc ccaccgcgat gctggagcgc cggggacgga
     5161 ctaaggcggt gacctatcac ctgtccaaag tgatcgccag cgacctgctc ggcaagtcgg
     5221 tctactcagc tgggcgaggg atcaacccgt tgcgctacgc cgagatggtc cgcgtatttc
     5281 tggaggatca tcggatgatc acgccgaagc aatgccgtga gttgctgcat ctgggcgagt
     5341 cgcagtcggc tcgagtggag atttcgcgtc tgctccggaa gtggagtgcc gccgatggat
     5401 tcttaacccg tgaagggaag ccgccgtccg tcgtttacca cctgaaggag gggaacccat
     5461 aactggggtg ttagcagact cccactaagt cccatgaacg cccactaact ccggcaaact
     5521 ccggcaaaat tcggcaaacc agcacaatgc aacatgaccg ccgcccaatc atagtgtgat
     5581 gacagctctt ggtgagtgtt agcaagtgtt agcaagtgtt agtctgccct accacagatt
     5641 ctatccccat gcccctcaac gaatccactg tcgaagaagc cgcgctcggc tggttctcgg
     5701 acctcaacta tgccgtgggc cacggaccgg cgatggcgcc gggcgagccg caggccgagc
     5761 gggacacgtt tggcgaagtg gtgctggtgg accggctgcg agccgcaatc cacaagctca
     5821 atcccgaagt gccggcagac tcacaggagg aggcgctgcg aaaggtgctg cgactggcca
     5881 cgccgtcgct ggtgcagagc aaccgagcct tccatacctt gctccgcaat ggcatcgacg
     5941 tggaatatgc ccggacagac gggacggcga agcacgacaa ggtctggctg gtcgatttcg
     6001 aaaacgagcg cgccaacgac tggctggcgg tcaatcaata caccgtcatc gaggcggggc
     6061 acaatcggcg agccgacatc gtggtcttcg tcaacgggct gccgctcggg ctgatcgagc
     6121 tgaaaaacgc cgccgatgga gaggccacga tctggagcgc ctacgcccag ctacaaacct
     6181 acaaggcgga ggtcccttcc ctgctccact acaacgcgtt gctaatcgtc agcgacgggg
     6241 tggaggcgcg ggtgggttcg ctcaccgcca atcaggaatg gttcaaggtc tggcgcacca
     6301 tcgagggaga tggcgatgcc cccaagtcgg ccctggagct ggaggtcctc atgcgcgggg
     6361 tctttgagcg gcggcggttc ctccaactgc tgcagcactt catcgtgttt gaggacgacc
     6421 ccgataccgg cgccgtccac aagatcatcg cgggctatca tcaatttcac gcggtcaacg
     6481 ccgccatcga cgaaaccgtg cgcgcgagcg gcatgggcgt ggcgggccag gtggacgaag
     6541 atccgggcac ctactggagc ggcaaaatgt cgggcggcaa accgggcgat cgtcgcgccg
     6601 gcgtggtctg gcatacgcag ggcagcggca agagtttcac catgctcttc ttcgccggtc
     6661 gcgtgatccg cgaggccgcg atgcagaacc ccaccctcgt cgtcctcacc gaccgcaacg
     6721 atctcgacga ccagctcttc ggccagttcc agcgctgcca cgaaatcctc ggtcaaacgc
     6781 ccgtgcaggc gccgagtcgc gaaaaactgc gcgagctttt ggccgtcgcc agcggcggcg
     6841 tggtcttcac cacgatccag aagttcatgc cggaaaaggg cgagaagatg ccctgcctga
     6901 gccagcggcg aaacatcgtg gtgatcgccg acgaagccca ccgctcgcag tacgacttga
     6961 tcgacgggct ggcgcgccac ctgcgcgatg cgttgccggg ggcctcgttc atcggcttca
     7021 ccggcacgcc catcgaaaag accgacgcca acacgcgggc ggtgtttggc gactacatct
     7081 ccatctacga catccagcgc gccgtcaccg accaggccac ggtgcccatc tactacgaaa
     7141 gccgcatcgc caagctgggg ctcaatacca gcgagctgcc caagctcgac gcggagtttg
     7201 aagagatcac cgagggcgag gaatcgaccc ggaaagagaa gctcaagacg aagtgggcgg
     7261 cgctggaagc gctcgtgggc gacccgaaac gcctggcact cgtcgccgcc gatctggtcg
     7321 cgcacttcga aaagcgcagc gaggccaata acggcaaggc catgatcgtc tgcatgagcc
     7381 gccgcatctg cgtcgatctc tacgccgcgt tgaccaagct gaggccggag tgggccagcg
     7441 cgaaggacga cgatagcgaa gcggagaagg gcaaagcgtg cgtcgtgaag gtcgtgatga
     7501 ccggaagcgc cgacgatggc ccgggctggc agccgcacat ccgcaacaag gagaggcgcc
     7561 gcaagctggc caaccgtttc aaggacagca aagacccctt ccgtatcgtg atcgtgcgcg
     7621 acatgtggct gaccggcttt gacgcgccgt gcctgcacac gatgtacgcc gacaagccga
     7681 tgcagggcca cggcctcatg caggccatcg cacgggtgaa ccgcgtattt cgcaacaaac
     7741 cgggtggcct cgtggtggat tacctcgggc tggcggaaca gttgaaaaaa gccctcgcca
     7801 cctacaccga gagcggcggc aagggcgacc ccacctacga cacccgccaa gcgatcgccg
     7861 tgatgctgga gaagtacggc atcgcctgcg acatgttgca tggcttcgac tgggcgaagt
     7921 ggacgaccgg aacccccgcc gaaaagctcg gcctcatccc cgccggccag gaacacatcc
     7981 tcacacagga cgagggcaag cggcgctgga ttcaaaacac catcgagcta tcccgcgcct
     8041 tcgcactctg cgccgccagc gacgaggcga ccgagatccg cgacgacgtc agtttcttcc
     8101 aagccctgca ggccacgctc agcaaaaaaa cgacgacgac gcagaaaacc ccggagcagc
     8161 tcgacgccgc ggtacgccag ctcgtgagcc aagcgattac gaccgagggc gaagtgatcg
     8221 acgtgttcac cgccgccggg ctgcccaaac ccgacatcgg catcctcagc gagcagttcc
     8281 tcgccgaagt gcgcggccta aagtacaaaa acgtcgccgc cgagttgttg gagaaactgc
     8341 tcaacgacga aatcaaggtg cgtgggaagc gcaatctggt gcaggcgcag gtcttttccg
     8401 acaagctcag gcaggcgctc aatgcctacc acaaccgggc catctccacg gtcgagttca
     8461 tcgacgagct gatcaagatg gcgaaacagc ttagccagat cgatcagcac aacgaggcgc
     8521 tcaaactctc gccggaggag gtcgctttct acgacgccct cgccgccaac gagagcgcca
     8581 aacaggtgat gggcgacgaa acacttcgcg tgattgcgac cgaactcatc acccaggtgc
     8641 gcaagagcgt gaccatcgac tggacgatac gggaaagtgc ccaggcaaaa atccgcgtgc
     8701 tggtaaaacg catcctcaat cgtttcggat atccgccaga catgcaggat gccgccgtca
     8761 aaacggtctt ggaacaagcc aagctgctct gcgccgagtg ggccgcctaa gccattaggg
     8821 aaccgctctt acgtggcgca ggtttccgtc tgcttttcct ggagggctgc ggcgctgcct
     8881 gccaacaccg gccacgcctg ccgacgtggc cgggcccggc attgggcagg gacgttcggt
     8941 gccggccgca gccaaccctg cggcagcagg tttagcggct gttccaacgg tggatttcca
     9001 gcagcgacgt gatgccgaaa gactgcctct ggccggcgcg gtggcaagcg ccgccaaacg
     9061 gaggcggcca accctcatca agctcggcag gcggggaggc gccttcgcct cgatctgccc
     9121 ggattcgtga agatctgtgg ccattcgtgg atgcctgctt tcccggagca gactggaagt
     9181 ctgcgctaca ccaaaccacc gcgaccccgc cccttttcac ttttcactct tcccccgccc
     9241 cggctctcac gagtgaacag ggttttcgtt gcccctggtg aagcgggggc gcttgctcgg
     9301 cgctttcgtc cgggccacgc ggtccggcgc cccactccca ctttccggtc atgcatctgc
     9361 acgcgctcga tatcgcaatc atcgtcggct atttcgtcct cgtcctctgc ctgggcctct
     9421 gggtctcgcg caagggcacc aaggatctcg actcgtactt cctcggcggg aaatcgctgc
     9481 cgtggtacgt gctgggcgtc tccgatgcgt cgggcatgtt cgacatcgcg ggcacgatgt
     9541 ggctcgtcta tgcgctgttt t
//
LOCUS       DAOSIT010000019         9412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_6998_length_9412_cov_8.365288, whole genome shotgun sequence.
ACCESSION   DAOSIT010000019 DAOSIT010000000
VERSION     DAOSIT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6998_length_9412_cov_8.365288"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLL14_01035"
     CDS             <1..258
                     /locus_tag="PLL14_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG02529.1"
                     /translation="YLHNPKPVYPAASRRLGEEGRVLLRVRVSAQGLPLSVDVRQSSG
                     SPRLDEAARAAVERWRFVPAKQGTEAVESTVVVPLQFTLDS"
     gene            272..1006
                     /locus_tag="PLL14_01040"
     CDS             272..1006
                     /locus_tag="PLL14_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG02530.1"
                     /translation="MRMEMGLAHFWAQGDLLTQAVAILLVLLSVASWCVIAAKAHSVW
                     QARRCHGRALAAFWGAPSLPAAIEAIRREDRTSILASLAVAGANAAGLHREHSDRGIG
                     AGVSVSDFVTRSLRSQIVEAQASIESGLTFLASVGSTAPFIGLFGTVWGIYHALVGLS
                     GATQVVLDKVAGPVGEALIMTAAGLFVAIPAVLAYNAFTRGNRLTLARLDGFAHDLHA
                     YLTTGLRGRPGDKLVDLGAVRAGRGG"
     gene            987..>1339
                     /locus_tag="PLL14_01045"
     CDS             987..>1339
                     /locus_tag="PLL14_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG02531.1"
                     /translation="MPAVAAEGEGAMAFGSFENSGASQPMAEINTTPLVDVMLVLLVI
                     FIITAPLFHEAVPIDLPRVDASRLDDRPRIVSVAINAAGQVFVDGREVARGELPARLA
                     AIGAAGQPELHLRADR"
     assembly_gap    1340..1439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1440..1934)
                     /locus_tag="PLL14_01050"
     CDS             complement(1440..1934)
                     /locus_tag="PLL14_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG02532.1"
                     /translation="MPVVLGMALLGCGSAATSPPVPELQAAPGLSADSDKPLAAPPTE
                     GTAAAALEDEHSIYFASGATRVDAAGQQKLRRHAARLQENPAQVLTLVGHTDDVGSSS
                     YNLAVAEQRIEAVARLLRAYGVPRKQMHPVRSYVVGAGKSAPTCRTAACRQKMRRVEL
                     IYAH"
     gene            2266..3039
                     /locus_tag="PLL14_01055"
                     /pseudo
     CDS             2266..3039
                     /locus_tag="PLL14_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
     gene            3053..4327
                     /locus_tag="PLL14_01060"
     CDS             3053..4327
                     /locus_tag="PLL14_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG02533.1"
                     /translation="MFSHLTMPVRVAWEDLQERLFFLPPFPDELDPLALFSMLLVVGL
                     LVGEWLRATLGWPKVIGYVLAGTLFGPSVLGWISIEALAQIRPIADAALGILMLEIGR
                     RLDLRWLRDNHELLRATLGEITLSFATIFLFAWAVVGLIPAWAAAAAAVTMASAPAVV
                     LLTVEESGAQGQVTERIILHTALSAAASFVVFAVVVGVVHAEYSEHWANAVVHPLWVV
                     VGGLIIAWLMARIALLVAALLPKRSLAQVFILVATALLAVGTARMLAVPVFLTLFLMG
                     VILSCTDRQKTLSYTHLPEGHWLLAIILFVVVGASLPWPDFTWLIGLQAIGLLGVRVL
                     AKIAALAWAGGGLTGAKRVLVGIGIQPLSATAVFMAYEIAGLYPEIGRSSLSLPLFAA
                     AIMELVGPALCRLALVRAGETVDTEKSTGGVA"
     gene            4324..5478
                     /locus_tag="PLL14_01065"
     CDS             4324..5478
                     /locus_tag="PLL14_01065"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HOG02534.1"
                     /translation="MNPAIAVFSHSEPLTLGVELELQLLSPRDFDLTRGATDLLGSMD
                     YDGHCGAIKLEITESMIEVSTLPQRQVDGIAADLLGLSQVLVRDCVRNHIVVSGGGTH
                     PFHHWPDRRICPGERFDEISHRYGYLAKQFTVFGQHIHVGCTSGDEAIWLTQALGPYV
                     PIFIALSASSPYVDGVDTLFQSARLNAVSAFPLSGQCPELRDWAGFIEHFSFLQSCGI
                     ARSIKDLYWDIRPKPEFGTVEIRVCDTPLTVERAAALAALAQSLVHHLLRTRPRLDAQ
                     GHLQVARYNKFQACRYGFDAQISDPVGRRQIPLRTLARELLILLAEDARVLGCWPWLE
                     LLDAALITGVSDADWLRGRQARHGNLNDVVCAAAMRFSSTASEAVAGRSR"
     gene            5475..6737
                     /locus_tag="PLL14_01070"
     CDS             5475..6737
                     /locus_tag="PLL14_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG02535.1"
                     /translation="MKYWLALLPLLLPLAWSLPALAQCTVEPLPRSSRSLAHWIDQDN
                     WLAPENLRFGLQSAGSFMPLVKLAGRAAAPLPAPLPRQIDLDKIRTIDPLDQQKRTVG
                     FLLESRLYADGLLVLRDGKVFGERYWNGLAAQQPRLLLGATRPLLSLLGAIAVAQGKL
                     APDRSVLRYIPALGAQTGLRKLSMRRLIAGESSFEWSPQELADWQAAGGWTSGGQTGG
                     IRGWLSQSGRWDRNSAAEPLFGVAGPEGELLAWALAESYRLPLARIFCDELFGRLRSE
                     NAALWLTDAQGTELSTGLALSLRDFARLGQLLIEARNSSGRSRIPRWFIETLTASAGL
                     RTAEATELAGLKKGSEFRYGFVHLGGTANRIAILGPYGNSLYVDLDRRLVIAIFAAYP
                     RNQSAGMRATLEQVWDTLGAATQPGGRR"
     gene            complement(6840..7010)
                     /locus_tag="PLL14_01075"
     CDS             complement(6840..7010)
                     /locus_tag="PLL14_01075"
                     /inference="COORDINATES: protein motif:HMM:NF021190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HOG02536.1"
                     /translation="MVDSLDPERVLPGDAVIGTLPIHLAARQTVTRFLVDSLAGHPGY
                     CRAEWRGQFGIA"
     gene            7441..8037
                     /locus_tag="PLL14_01080"
     CDS             7441..8037
                     /locus_tag="PLL14_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02537.1"
                     /translation="MKKLAMSALLGAMVLASANASATVINFGSLGLAEGQSVQGSTFS
                     GVTFTGQAGTLIYTGSYGAGLYDGLGAANDITLSFSSGVSSLSLRAGDGGGDLDAFGV
                     SLYEFGTSNFLGYFTTPLFGGPSEPEWYTLNLAGLGLIGKAVFDPGNSGVLPGLPSGF
                     GGVILTTVSFDEHAVPIPGTLALALLGLAGLASARRRS"
     gene            complement(8263..>9412)
                     /locus_tag="PLL14_01085"
     CDS             complement(8263..>9412)
                     /locus_tag="PLL14_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG02538.1"
                     /translation="TLELGIDVGDLDYVIQIDAPSTVSSFLQRMGRSGRRPGSRRNCL
                     FLTTTDEAFLLACAITTLWREKYVEHIEPPPLPWHMVAQQMLALVLEFPGLPAHKVVG
                     VARKQFPDLDPATLATVFDFMVGKGILFVSSGLASMGPEGEKLFGRAHFLELLSAFAS
                     PMVLAARYGSSELGYVDPMAVQPLKEGPTVLLLGGRSWRVISVDWSRRMVYLEPTDEK
                     GTSRWLGTSRWLGFTVCQAMRRVLLQEDDAKLGLSRRGTRQLDEVRALITAPERQGGL
                     LLERLPSGRLRWWTFAGGAVNSALALRLGEVGDTRVDDLWIETAPGVSVPTLIQHPVE
                     QSAMSAFAEHLAKRCELKFAACLPTDLAAAVLVGRSLDEANLRRLVSG"
BASE COUNT         1548 a         3006 c         3005 g         1753 t
ORIGIN      
        1 tacctgcaca atcccaagcc ggtgtatccg gcggcgtcgc gccggctcgg cgaggaaggg
       61 cgggtcctgc tgcgggtccg ggtcagcgcg cagggcctgc cactgagcgt ggacgtcagg
      121 cagtcgagcg gctccccgcg tctcgacgag gcggcacgcg ccgccgtcga gcgttggcgc
      181 ttcgtgccgg cgaaacaggg gacggaagca gtcgaatcga cggtggtggt gccgctgcaa
      241 ttcacactgg acagttgaag aggcaaacac aatgcgaatg gagatgggtc tggcccattt
      301 ttgggcccag ggcgacctgc tgacgcaggc cgtggcgatc ctgctggtgc tgctgtcggt
      361 ggccagctgg tgcgtgattg ccgccaaggc gcacagcgtc tggcaggcca ggcgttgtca
      421 tgggcgggcg ctggccgcct tctggggcgc gccgagcctg ccggcggcga tcgaagcgat
      481 ccgccgcgag gaccgcacca gcatcctggc cagcctggcg gtcgccggcg ccaacgccgc
      541 cggcctgcat cgcgaacatt ccgatcgcgg catcggcgcc ggcgtcagcg tcagcgactt
      601 cgtcacccgc tcgctgcgca gccagatcgt cgaagcgcag gcgagcatcg aatccggact
      661 cacctttctc gcctcggtcg gatcgacggc gccgttcatc ggcctgttcg gcacggtgtg
      721 gggaatctac cacgccctgg tcgggctctc gggtgcaacg caggtggtgc tcgacaaggt
      781 cgccgggccg gtcggcgaag cgctgatcat gaccgccgcc ggtctgttcg tggccattcc
      841 ggcggtgctt gcctacaacg ccttcacccg cggcaaccgc ctgacgctgg ctcgcctcga
      901 cggcttcgcg cacgacctgc acgcctacct gaccaccggc ctgcgcggtc gtccgggcga
      961 caagctggtc gacctcggcg ccgtgcgtgc cggccgtggc ggctgagggg gagggcgcga
     1021 tggcctttgg ttccttcgaa aacagcggcg ccagccagcc gatggccgaa atcaacacca
     1081 cgccgctggt cgacgtgatg ctggtgctgc tggtgatctt catcatcacc gcgccgctgt
     1141 tccacgaagc ggtgccgatc gacctgccgc gtgtcgatgc cagccggctc gacgaccgac
     1201 cacgcatcgt gtcggtggcc atcaacgccg ccggccaggt cttcgtcgat ggccgggagg
     1261 tcgcgcgcgg cgaactgcct gcccggctgg ccgccatcgg cgccgccggc cagcccgaac
     1321 tgcacctgcg cgccgaccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1441 cagtgcgcgt agatcaactc gacccggcgc atcttctgcc ggcaggccgc tgttcggcag
     1501 gtcggcgccg attttccggc accgacgaca tagctgcgca ccggatgcat ctgcttcctg
     1561 ggcacgccat aggcgcgaag gagtctggcg accgcttcga tgcgctgctc cgcgaccgcc
     1621 agattgtatg agctgctgcc gacgtcgtcg gtatgaccga ccagggtgag cacctgtgcc
     1681 ggattctcct gcaggcgtgc ggcatggcgg cgcagctttt gctgccccgc cgcatcgacg
     1741 cgcgtggcgc cggaggcaaa atagatgctg tgttcatctt caagtgcagc ggcggccgtg
     1801 ccctcggttg gcggcgcagc cagcggcttg tccgagtccg cggacaggcc gggcgcagct
     1861 tgtagctccg ggactggcgg tgaggtggcc gcgctgccgc agccgaggag cgccatgccg
     1921 aggacaactg gcacagtata ccgccagggg tggcggtcgc ttggtggtcg caggggagtc
     1981 gtcatctggc gtcggcgatt gcgcaactgg ggcgcgttaa ctatattgaa caaaaatgta
     2041 gcattaatca gtgcttcttg aattcgtttc cggtaatcgg cgcgcggggc cattttacga
     2101 cctcgcctgc tgagcacggt gtcgcgcctg cggttttccg accccctgct ggcccggaag
     2161 ggtgcccagg ggttgcggtg aggcaacgga tttgccgtcg gaccgcccgg cagacgacgg
     2221 agccgccgcc agccgagctt tcagtggaga tcagtcatga gcaggcgccc cctacgtatc
     2281 ggtctttccg cacgcatcta ccacccgcag gctggtgcca ccggcctgca gtcgaaatcc
     2341 ctgcaatatc ttgagcaatc ggtcgcgcac tgggtgatgt cccgtgacgt gatggtgttc
     2401 atgattccgg cggtcagtgg cgacgccacg gtgcatcgca gcagcatccg gctctcggac
     2461 tacccggact atctcgatgg cctgattctg caaggcggcg ccgacatcaa tccgcagagc
     2521 tacggggagc agccgttgca tccggactgg gtcggcgacc gggttcgtga cgcctacgag
     2581 atggaactgc tgcacgaatt catggaggcg ggaaaagccg tgctcggcat ctgtcgtggc
     2641 gcgcaactga tcaacgtggc gcttggcgga acactttatc aggacattgc gacgcaggtc
     2701 gacacggcca ccgagcacgt gcacgccaac tacgaccggc atacccatgc catcgaatgg
     2761 actcccaaat cggggatggc aaagctctat ccgggcgcga ccggcgggcg ggtggtgtcg
     2821 attcatcatc aggcgatcaa ggccctgggc cgggaactgc gggtcgaggc gcgcagtacc
     2881 gaggatggtt tgatcgaaac gatacgcctg caaggcagac cgtacgtcgt cggcctgcag
     2941 tggcacccgg agttccatcc cccaggtagc gaggatctcc tggactgtac gccgatcctc
     3001 gacgaatttc tggtggccgc acgcggtcga ctgtggtgag cagagcgcgg cgatgttcag
     3061 ccatttgacg atgccggtac gcgtcgcctg ggaagatctg caggagcgcc tgtttttcct
     3121 gccgcctttc cccgatgagc tcgatccact ggccttgttc agcatgcttc tggtcgtcgg
     3181 tctgctggtc ggcgagtggt tgcgcgccac gctcggctgg cccaaggtca tcggctacgt
     3241 cctcgccggg acgctgttcg gaccctcggt gctgggctgg atcagtatcg aggccctggc
     3301 ccagatccga ccgattgccg acgcggcgct cggcatcctg atgctcgaga tcggccgccg
     3361 cctcgatctg cgctggctgc gcgacaacca cgaactgctg cgggcaacgc ttggcgagat
     3421 cacgctgtcg ttcgccacca tctttctctt cgcctgggcc gtggttggcc tgataccggc
     3481 atgggcggcc gctgcggcgg cggtgaccat ggcctcggcg ccggcagtgg tcttgctgac
     3541 cgtcgaggaa tccggtgcgc agggacaggt gaccgaacga atcatcctgc acaccgcgct
     3601 cagcgcggcg gcatcattcg tcgtgtttgc cgtcgtcgtc ggcgtggtgc atgcagaata
     3661 cagtgagcac tgggccaatg cggtggtaca tccgctatgg gtcgtggtcg gtggcctgat
     3721 catcgcctgg ctgatggcca ggatcgcgct gctggtggcc gcgctgttgc cgaagcgctc
     3781 gctggcacag gtgtttatcc tggtggcgac ggcgttgctc gccgtcggta ctgcgcgcat
     3841 gctggcggtg ccggttttcc tgaccctctt cctgatgggc gtgatcctgt cgtgcaccga
     3901 ccgacaaaag accctcagct atacgcacct ccccgaaggt cactggcttc tggcgatcat
     3961 tctctttgtc gttgtcggcg cttccctgcc ctggccggat ttcacctggc tgattggact
     4021 gcaggccatc ggcctgctgg gcgttcgggt gctggccaaa atcgcggccc tggcctgggc
     4081 cggcggtgga ctgaccggcg ccaaacgggt gctggtaggc atcggcatcc agcccttgtc
     4141 ggcgaccgcc gtgttcatgg cctatgaaat tgccggcctt tatccggaga ttggtcgttc
     4201 gtcgttgtca ctgccgctgt ttgccgcggc gatcatggaa ctggtcggtc ccgctctctg
     4261 ccgtcttgcc ctggtacgcg cgggagaaac ggtcgacacc gaaaaaagta cgggaggtgt
     4321 ggcatgaatc cggccatcgc cgttttcagt cacagcgagc cattgaccct cggcgtcgag
     4381 cttgagttgc agttgctctc cccgcgcgat ttcgacctga cgcgcggcgc taccgacctc
     4441 ctcggcagca tggactacga tggccactgc ggtgccatca agcttgagat caccgaaagc
     4501 atgatcgaag tcagcaccct gccgcagcgc caggtcgacg ggattgccgc ggatctgctg
     4561 ggcctcagcc aggtgctggt ccgcgactgc gtgcgcaacc acatcgtcgt cagcggtggc
     4621 ggtacgcatc ccttccatca ctggccggat cgccgcatct gtccgggcga gcgcttcgac
     4681 gaaatatcgc atcgctatgg ctacctggcc aaacaattca ccgtcttcgg gcagcatatc
     4741 catgtcggct gcacctcggg tgacgaagcg atctggttga cgcaagccct gggaccttac
     4801 gtgccgatct tcatcgccct gtccgcctcc tcgccctatg ttgatggcgt ggataccctg
     4861 ttccagtccg cccgcctcaa tgccgtctcg gctttccccc tgagcgggca atgcccggaa
     4921 ttgcgcgact gggccgggtt catcgaacac ttttcgtttc tgcagtcctg tggcattgcg
     4981 cgcagcatca aggacctgta ctgggacatt cgtcccaaac ccgaatttgg caccgtcgaa
     5041 atccgtgtct gcgatacgcc actgaccgtc gagcgcgccg ctgcgctggc ggcactggcg
     5101 caaagcctgg tccaccatct cctgcggact cgtcccaggc ttgatgccca agggcatctg
     5161 caggttgccc gctacaacaa gttccaggcg tgtcgctatg gtttcgacgc gcagatctcc
     5221 gatccggtcg gcaggcgaca aatcccgctg cgaacgctcg cgcgcgaact ccttatcctg
     5281 ctggccgagg atgcgcgtgt gctgggctgt tggccctggc tggaactgct cgatgccgcg
     5341 ctgatcaccg gcgtctccga cgccgactgg ctgcgcggac gccaagccag gcacggcaat
     5401 ctcaacgatg tggtgtgtgc tgccgcgatg cgtttcagca gcaccgccag tgaggccgtc
     5461 gccgggaggt cccgatgaag tactggctgg cgttgctgcc gttgctgctt cccttggcgt
     5521 ggtccttgcc ggcactggcg caatgcactg tcgaaccgct gccccggagc agcagaagcc
     5581 tcgcccactg gatcgaccag gacaactggc tggcgccgga aaacctgcgc ttcggcctgc
     5641 agtcggctgg cagcttcatg ccgctggtga aactggcggg tcgcgcggca gcaccgcttc
     5701 cggctccgct accgcgtcag atcgacctcg acaaaatcag gacgatcgac ccgctcgatc
     5761 agcagaagcg gacggtcgga tttctgctgg aaagccgatt gtatgccgat ggcctgctgg
     5821 tgctgcgcga cggcaaggtg ttcggcgagc gttactggaa tggcctcgcg gcgcagcaac
     5881 cgcgcctgct gctgggcgcc acaaggccgc tgctgtcgct gctgggggcg atcgcggtcg
     5941 cccagggcaa gcttgcgccg gatcgctcgg tgctccgcta catcccggcc ctgggcgctc
     6001 aaacgggctt gcgcaagctt tcgatgcggc gcctgatcgc cggagaaagc agcttcgagt
     6061 ggtcgccaca ggaactcgcc gactggcagg cagcgggtgg atggacgtcc ggcgggcaaa
     6121 cgggggggat tcgcgggtgg ctcagtcagt cgggccgctg ggacaggaat tccgctgcgg
     6181 aaccgctttt cggcgtcgcc ggaccggagg gtgagttgct ggcctgggca ctcgccgaaa
     6241 gttatcgcct accgctggca cgaattttct gcgacgaact gttcggcaga ctccgctcgg
     6301 aaaacgcggc cctctggctg accgacgcgc agggaaccga actgtccacc ggattggcgc
     6361 tctcgcttcg cgactttgcc cgtctcgggc agttattgat cgaggccaga aacagcagtg
     6421 gacgcagccg gattccgagg tggttcattg aaacgctgac ggcctccgcg gggttgcgca
     6481 ccgccgaagc cactgaactt gccggcctga agaaaggaag tgaattccgc tatggctttg
     6541 tgcaccttgg cggaacagcg aaccgcatcg ccatcctggg cccctacggc aacagccttt
     6601 atgtcgatct tgatcgccga ctggtgattg ccatttttgc cgcctacccg aggaatcaaa
     6661 gcgccggcat gcgcgcaacc ctcgaacagg tgtgggacac cctgggtgcg gccacccagc
     6721 cgggcggcag gcgctgatcg cggtggcacc aggcacgcgc ccgcctggtc gctgcactgc
     6781 cgcgccggac caaggttccg gacttcctgc cgtggctgcc agcgcgcctt gccgacgggt
     6841 cacgcaatgc cgaattggcc gcgccattca gcgcggcaat atcctgggtg ccctgccagc
     6901 gaatcgacca ggaatcgggt gacggtctgc cgggcggcga gatggatcgg cagtgtgccg
     6961 atgacagcgt cacccggcag cacccgctcg ggatcgaggc tgtcgaccac gccattgccg
     7021 cagacctcaa ttccggcctc gattgcctat tgcaccgcac aaagcgctgc cgcccggcct
     7081 gagcatcccg tgaccgctgc ttcggccgcc ggcaaaggcc atggggtgca gtaacgcgta
     7141 catgtgaaag gcattccgac aacggatcga gccttgcagc gcacaactgt cgaaaaactt
     7201 tacattggcg atgtcgtcac cccagcacag cgcgatgggg atttgattca atagccattt
     7261 atggcttgtg cgcaaaatgg attgattctt gcttatggtt tgtgggcgca cagaatatcc
     7321 cgcggccgtg cttgcatcgc ttgactccat gtttcggcca gtaggcatta tgtagacggg
     7381 aagattgtcg tcaaatgagt agcttgacct gtggattgca cttctctctt gggggtaatg
     7441 atgaaaaaac ttgcgatgag cgccttgctt ggcgcaatgg ttcttgcttc ggccaacgcc
     7501 tcggcaacgg tgatcaattt cggttcgctg ggcctggctg agggccagag cgttcaaggt
     7561 tctacgttca gtggcgtgac ctttaccggg caagcgggca ctttgatcta caccggtagc
     7621 tatggggcgg gcctttatga cggtcttggc gccgcgaatg atattacgct gagcttctcc
     7681 agcggggttt cctccctgtc tctgcgcgct ggcgatggtg gcggcgacct cgatgccttt
     7741 ggcgtttcgc tttacgaatt cggtaccagc aacttcctgg gttatttcac gaccccgcta
     7801 ttcggcggac cgagcgagcc tgagtggtac acccttaacc tggcaggcct cggcctgatc
     7861 gggaaagcgg tgtttgatcc aggcaacagc ggtgtcctgc cgggactgcc aagcggcttc
     7921 ggtggcgtca ttctcactac ggtaagtttc gacgaacacg cggtacccat ccccggcact
     7981 ctggcgcttg ccctgctggg tctggctggc ctggcctcgg ctcgccgtcg aagctaagcc
     8041 tccgggtggc tttcgggaag cctggcgaaa cgggcaaggc ttcctgagcg aatttcattc
     8101 gactgggcca aaacggccgt tcgcgtttgt caatgaccga atgtccggtc tcacatacac
     8161 agcggacttg agagggtgaa acgagagtgt cggctttggc cgatcaatag tctggcatcc
     8221 aacagggcca gcgtccgcct gcaatatgcg tcatggcccc cgctatcccg acaccagccg
     8281 ccgcaagttc gcttcatcca gggacctgcc cacaagcacg gcagccgcca ggtcagtggg
     8341 cagacaagcg gcgaacttca actcacagcg ctttgccagg tgctcggcaa acgcactcat
     8401 cgccgactgc tcgaccgggt gttgaatcag cgttggcaca gagacgccag gcgccgtctc
     8461 gatccacaga tcgtccaccc gggtgtcccc gacctcgcca agtctcagcg ccaatgcact
     8521 gttgaccgct ccgccggcaa acgtccacca gcgaagccgg ccagatggca ggcgctccag
     8581 cagaaggccc ccctggcgct cgggcgcggt gatcagcgca cgaacctcgt cgagctgccg
     8641 agtgccgcgc cgcgataggc ccagtttcgc gtcgtcctcc tgcaacaaca cgcggcgcat
     8701 cgcctgacaa accgtgaaac ccagccagcg cgacgtgccg agccagcgcg acgtgccttt
     8761 ctcgtcggtc ggctccaggt acaccatgcg cctggaccag tcgactgaga tcacccgcca
     8821 gcttcggccg ccaagcagga gaacggtcgg cccctccttc agcggctgca ccgccatcgg
     8881 gtcgacgtaa cccagctcgc tggaaccgta acgggccgcc agcaccatcg ggctggcaaa
     8941 ggccgacagc aactccagaa aatgagcgcg tccgaacagc ttctcgcctt caggccccat
     9001 cgaggcaagg ccgctgctga caaacaggat gcccttgccc accatgaagt cgaacaccgt
     9061 cgccagcgtc gccggatcga gatcggggaa ctgcttgcgc gccacgccga cgaccttgtg
     9121 cgccggcagg ccggggaact ccaggaccag cgccagcatc tgttgcgcca ccatgtgcca
     9181 cggcagcggc ggcggctcga tgtgctcgac atacttctcg cgccacagcg tggtaatcgc
     9241 gcaggcaagc aggaaggcct cgtcggtggt ggtcagaaac aggcagttgc ggcggctgcc
     9301 gggacgccga ccgctgcgcc ccatgcgctg caggaacgaa gacaccgtgc tcggcgcgtc
     9361 gatctggatc acgtaatcga ggtcgccgac atcgatcccc agttccagcg tg
//
LOCUS       DAOSIT010000020         9354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7038_length_9354_cov_8.706097, whole genome shotgun sequence.
ACCESSION   DAOSIT010000020 DAOSIT010000000
VERSION     DAOSIT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_9354_cov_8.706097"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="lpxK"
                     /locus_tag="PLL14_01090"
     CDS             complement(<1..626)
                     /gene="lpxK"
                     /locus_tag="PLL14_01090"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG02539.1"
                     /translation="MAVSGLAQRLPGWWYQRRLVVALWPLLPLSWLFGVLGRLRRGLY
                     RCGVLRTYHLPVPVVVVGNLTVGGSGKTPLVLWLVSRLRDHGWFPGIISRGYGGTGEP
                     VRPVLTSSPCSVVGDEPLMLARRSGVPVFVGRDRVAVGQALLAAHPECNVIVADDGLQ
                     HYRLQRAVELVVFDGRGIGNGHLLPAGPLREPLRRLSGVAAVIWNGLP"
     gene            complement(626..1042)
                     /locus_tag="PLL14_01095"
     CDS             complement(626..1042)
                     /locus_tag="PLL14_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOG02540.1"
                     /translation="MNFRRGRGSDVPEINLIPLIDVLLVIIIFLMLTTTYAKFSGLEI
                     SLPTADANKQTEQPNEINVALTAAGQVLVNKIPLATASVPAISDALRRAAGDTREPVI
                     VINADARANYQSVVDIMQAAQTAGYPRISFATQSPH"
     gene            complement(1053..1661)
                     /locus_tag="PLL14_01100"
     CDS             complement(1053..1661)
                     /locus_tag="PLL14_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG02541.1"
                     /translation="MFSIIQAAGWPIWPLLMASIIAVALIIERLAALRRSKVVPPGLL
                     QRVVAEYRQNGVTGAMVGDLEAHSPLGRVLGAGLRNVGSSREIMKEAIEEAGSVVSHD
                     LERYLTTLGTIAAISPLMGLFGTVVGMIEIFGSQAPSGSNPIQLAHGISVALYNTGFG
                     LLIAIPSMIFWRHFRALVDSFVIEMQQQAVRLVEVLHGQRKS"
     gene            2001..3410
                     /gene="xseA"
                     /locus_tag="PLL14_01105"
     CDS             2001..3410
                     /gene="xseA"
                     /locus_tag="PLL14_01105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG02542.1"
                     /translation="MSDIACAHIGLPGSAPVIPVSLLNRLARERLEATFPLCWVAGEI
                     SNLSHAASGHLYFSLKDSAAQVRCVMFRSRAQILGWRLENGQQIEARVLVTLYEARGD
                     FQLNVEAARRGGIGNLYERFLRLQDQLASEGLFDSAAKRPLPTFPQRIGIITSPQAAA
                     LRDVLSTLRRRAGHVSIVLYPTPVQGDGAARQIAEAILGAGARRECDLLIVCRGGGSI
                     EDLWAFNDEAVARAIRACSLPVICGIGHETDFTIADFAADQRAPTPTAAAEIAAPERA
                     ALLARLAAGELALRRQIEQQLNQRGQHLDWLAHRLQHPALYLARQHERLENLQRRLGA
                     GLLQASARARTMLAGLNRRLLLARPDPVRQAGHLETLAPRLRGAWSRTLQGCFADLAR
                     LSAGLEHLNPTAVLARGYSIVTNAQRQIVRESRSLEPGQQIVVFFHRGSAEATVRAIS
                     EDLAGPLAGGGIASTASAD"
     gene            3501..4088
                     /locus_tag="PLL14_01110"
     CDS             3501..4088
                     /locus_tag="PLL14_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOG02543.1"
                     /translation="MEHQLPQLPFALDALAPHMSRETLEYHYGKHHQAYVTNLNNLIK
                     GTDYEALDLEAIVKKAPAGGIYNNSAQVWNHTFFWNCLTPNGGGAPGGELAAAIDGKW
                     GSFADFAKAFQASAVGNFGSGWTWLVKKADGSLDIVNMGAAGTPLTTGDKALLCIDVW
                     EHAYYIDYRNLRPKFVETFLGSLANWRFAEQNFAA"
     gene            complement(4118..4852)
                     /locus_tag="PLL14_01115"
     CDS             complement(4118..4852)
                     /locus_tag="PLL14_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HOG02544.1"
                     /translation="MTYCVAMRLDAGLVFLSDSRTNAGVDHINTFRKMTVIERVGERV
                     LVLMTAGNLSVSQSVVNILRERLDRSDQENLCSLPNLFEAARHVGSVVREVHQRDAAA
                     LQEFGIEFNASIIFGGQIGEEDPRLFNIYAAGNFIEASEDTPYFQIGESKYGKPIIDR
                     VVNRRTTFEEAAKCALISMDSTIRSNLSVGMPLDLLIYERDALRVRDHVSIGPQNAYF
                     NTIRTLWGERLRQAFTALPDPDWGSF"
     gene            complement(4916..5851)
                     /locus_tag="PLL14_01120"
     CDS             complement(4916..5851)
                     /locus_tag="PLL14_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOG02545.1"
                     /translation="MLSRTADHLYWMARYTERAENLARLLDVSYQMSLVPQSVASQNE
                     NWRAILALNSLEAAFALVYNEVNVENVLRFMVSDPSNPSSIYSCLRAARENAHAVRGT
                     LTAEMWESVNATWIELRQQNFEHILARGVSEFFDWVKLRSAVTRGVTFGTMLKDDALH
                     FIRLGGLLERGDNTARILDMQYHILRQNDDEGASDFYRWGALLRSLSAFQVYRKVYRD
                     AITPGRVAELLILRMDMPRSLHACADGIVRMLQLIANGASAETERQAGVLHARLHFAR
                     IDDVLAGGLHEYLTDFMDRIYELGDGISRDFLIPG"
     gene            complement(5870..>6316)
                     /locus_tag="PLL14_01125"
     CDS             complement(5870..>6316)
                     /locus_tag="PLL14_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOG02546.1"
                     /translation="PEMIRFYLGEEPKLNNVPTWMLRKPDDLKYVLAHLPELVVKEVH
                     GAGGYGMLVGPASTHAEIEDFRARILAAPEKYIAQPTLALSNCPTFVDAGIAPRHLDL
                     RPFVLSGKRGIGMVPGGLTRVALREGSLVVNSSQGGGTKDTWVLEN"
     assembly_gap    6317..6416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6417..7141)
                     /locus_tag="PLL14_01130"
     CDS             complement(<6417..7141)
                     /locus_tag="PLL14_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOG02547.1"
                     /translation="MNADFYNEMYDSAGTLRPHYSAFAEWLAGMPPERLERKRAEADT
                     AFHRVGITFAVYGEESGTERLIPFDIIPRIIPAAEWQALQAGLWQRVKALNAFLHDIY
                     HEQAILRAGVIPSEQVLNNAQFRREMMGVDVPSGIYAHIAGVDIVRAGAGESYVLEDN
                     LRVPSGVSYMIEDRKMMMRLFPELFSRHLIAPVQHYPDMLLENLRTVAPKGVADPTVV
                     LLTPGAYNSAYFEHTFLAQQMGV"
     gene            complement(7309..8604)
                     /gene="gltA"
                     /locus_tag="PLL14_01135"
     CDS             complement(7309..8604)
                     /gene="gltA"
                     /locus_tag="PLL14_01135"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOG02548.1"
                     /translation="MVSERKATLSIVGEPAVHFSVLAPTHGSDCLDIRTLGARTGRFA
                     YDSGFESTASCKSKITFVDGEKGELLHHGYPIKQLAEQCDFLDVAYLLKHGELADAGQ
                     KVAFESAIRKHTSVHEQMVKFYQGFRRDADPMAVMVGVVGALSAFNHDAADYFDTAQR
                     NLNFHRIVARMPTIVAMAYKYSIGEPFMNPRADLGYAANFLHMMFATPREEFRPNPVL
                     VRALDRLFIVFADQGQDASTSTVRMAGSSGANPFACVSAAIACLSGPAHSGASEACLA
                     MLQEIGDTSRLGGFVARARNKRNGFTFSGFGHPVYRHFDPRAQLLREVCDEVLQELGL
                     EQHRLFALAKALETLSLEDPYFVAEGLYPSVDFYSAIVQKALGIPTSMFACIQALART
                     AGWMAQWEEMLADSEYKIARPRQLYTGVNRRDLSAAAHR"
     gene            9031..>9354
                     /locus_tag="PLL14_01140"
     CDS             9031..>9354
                     /locus_tag="PLL14_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02549.1"
                     /translation="MNPPMVLPWLASKAGVSIRQAEALWPSACRQAAQITGQRDGSTY
                     WGTAVQTLLRLLQEERRQTVSLLTWPWLLLQSGVEHWSRLTRNWLAPIDASLHTWKLH
                     LEGSRH"
BASE COUNT         1822 a         2995 c         2843 g         1594 t
ORIGIN      
        1 tccggcagac cgttccagat caccgcggcc acccccgaca aacgccgcaa gggctcgcgc
       61 aaggggccag ccggcagcag gtggccgtta ccaatgccgc gtccgtcgaa caccaccaac
      121 tcgaccgcgc gctgcagacg gtagtgctgc aggccgtcgt cggcgacgat gacgttacat
      181 tcggggtggg ctgccagcag ggcctgcccg acggcgacgc gatcacgacc gacgaagacc
      241 ggcacaccac tgcgcctggc cagcatcagg ggctcgtcac cgaccaccga gcaaggtgag
      301 gaagtcaata ccgggcgcac cggctcgcca gttccaccgt agccgcgact gatgatgccc
      361 gggaaccagc cgtggtcacg caaacgactg accagccaca gtaccagcgg tgtcttgccg
      421 ctgccgccta cagtcaggtt tccgaccacg accaccggca ccggcagatg ataggtacgc
      481 agcacgccgc agcggtagag tccgcgccgc aaccggccga gcacgccgaa cagccaggat
      541 agcggcagca gcggccacaa ggcgacgacc aggcgacgct ggtaccacca gccggggagg
      601 cgctgcgcca ggccggaaac ggccatcagt gcggcgattg ggtggcgaag gaaatgcgcg
      661 gatagccggc ggtctgcgcc gcctgcatga tatcgacgac gctctgatag ttggcccggg
      721 cgtcggcatt gatcacgatc accggttccc tggtgtcgcc ggctgcgcgg cgcagcgcgt
      781 cgctgatcgc cggcacgctg gccgtagcca gcggtatctt gttcaccagc acctggccgg
      841 ccgccgtcag tgcgacattg atctcgttgg gctgctccgt ctgcttgttg gcgtcggcgg
      901 tgggcaggct gatctcgagc ccggagaact tggcgtaggt ggtggtcaac atcaggaaaa
      961 tgatgatcac cagcagtacg tcgatcagcg ggatcaggtt gatctcgggc acatcactgc
     1021 cacgaccgcg tcgaaagttc atcgccagcc cctcaagact tgcgctggcc gtgcagaacc
     1081 tcgaccaggc gcactgcctg ctgctgcatc tcgataacga aactgtccac cagagcacgg
     1141 aaatggcgcc agaagatcat gctcggaatg gcgatcagca ggccaaaacc ggtgttgtac
     1201 agggctaccg aaatgccatg cgcgagttgt atcgggttac tgccgctggg tgcctgcgaa
     1261 ccgaaaatct cgatcatgcc gacgacggtg ccgaacaagc ccatcagcgg gctgatggcg
     1321 gcgatggtac ccagtgtcgt caggtagcgt tcgagatcgt gtgacaccac gctgccggcc
     1381 tcctcgatcg cttccttcat gatctcgcgg gaactgccga cattgcgcag accggcgcca
     1441 agtacgcggc caagcgggga atgcgcctcg aggtcgccga ccatggcacc agtcacgcca
     1501 ttctggcgat actcggcgac gacccgctgc aacaggccgg gggggacgac tttgctgcgc
     1561 cgcagcgccg caaggcgctc gatgatcagc gcgacggcga tgatcgacgc cataagcaag
     1621 ggccagatcg gccaaccagc agcctgaatg atggaaaaca cgttctcctc caggagattt
     1681 tctcaggcgg gcactttagc ctgcgccgac gtttgcagca agccccggaa tcggcactgg
     1741 ccttcgccgg gatcgaccgg ctatccacaa catctgtgga taaccctgcc ccaaggctgt
     1801 tggggaacca gtcaagtcca gagcggaaaa ggagtttttc cgggtgccgg aaatcacggc
     1861 agcgggccgt cggatagcag tcgtcacgca gcgggcggcg gcgaccgcca gggacgtgtc
     1921 gtcagacctt gacctcccct gctgcggctg ccacggccca ttcccggcgc aagcggcagg
     1981 cagccatgta gaattggcgc atgtcagata ttgcatgcgc acacatcggg ctgcccgggt
     2041 cggcaccggt gattccggtt tcgctcctca atcgcctcgc gcgcgagcga ctcgaagcga
     2101 cctttccctt gtgctgggtg gctggcgaga tttccaacct cagccatgcg gcgtccgggc
     2161 atctctattt ttcgctcaag gacagcgccg cgcaggtgcg ctgcgtcatg ttccgcagtc
     2221 gcgcacagat tcttggctgg cgactggaga atggccagca gatcgaggcg cgcgtgctgg
     2281 tgacgctgta cgaggcacgc ggggatttcc agctcaacgt cgaagcggcg cgtcgtggcg
     2341 gcatcggcaa cctctacgag cgtttccttc ggctgcagga ccagctcgcc agcgaaggac
     2401 tgttcgacag cgccgcgaag cgccccttgc cgacgtttcc gcagcgcatc ggcatcatca
     2461 cctcgccgca ggcggcggcg ctacgcgacg tcttgagtac gctccgacgc cgcgccggac
     2521 acgtgtcgat cgtgctttac ccgactccgg tacagggtga cggggcggcc agacaaatcg
     2581 ccgaggcgat tctcggtgcc ggtgcgcgtc gggaatgcga tctgctgatc gtctgccgcg
     2641 gtggcggcag catcgaagac ctctgggcat tcaacgacga ggccgtggca cgggccattc
     2701 gtgcgtgttc gctaccggtg atctgcggca tcggtcacga aaccgatttc accattgccg
     2761 actttgcggc cgatcagcgg gcgccgaccc ccaccgccgc cgccgaaatc gcggctcccg
     2821 aacgggccgc gctgctcgcc cggctcgccg ccggcgaact cgccttgcgc cgtcagatcg
     2881 agcagcagct caatcagcgt ggccagcacc tcgactggct ggcacaccgg ctgcagcacc
     2941 cggcgctgta cctcgcacgc cagcatgagc gcctcgaaaa cctgcagcgc cgcctcggtg
     3001 ctggactgct gcaagccagt gcgcgcgcgc gcacgatgct ggcgggcctc aaccgtcgcc
     3061 tgctgctggc ccgtcccgat ccagtacgcc aggccggcca cctcgaaacg ctggcgccgc
     3121 gcctgcgtgg cgcctggagc aggacactgc agggctgctt cgccgatctc gcccgtctct
     3181 cggccggtct cgaacacctc aacccgaccg cggtcctggc gcgaggttac agcatcgtca
     3241 ccaacgcgca gcgtcagatc gtgcgcgaga gccggtcgct tgaacctggg cagcagatcg
     3301 ttgtcttctt ccaccggggc agcgccgaag cgacggtacg ggcaatttcc gaagacctcg
     3361 caggccccct cgcaggcggc ggcattgcca gcaccgcaag cgcagactag aatcgcctgt
     3421 tgaagcgcca gcgaagacgg ttcacagcag gcattaaatc agccctctta tccgcataac
     3481 gaccctcacg gagaacattc atggaacatc aattgccgca actgcccttc gccctcgacg
     3541 ccctcgcccc gcacatgtcc agggagactc tggaatacca ctacggcaag caccaccagg
     3601 cctacgtcac caacctgaac aacctgatca aaggtactga ttacgaagcc ctcgacctcg
     3661 aagcgatcgt caagaaggct ccggcgggtg gcatctacaa caactcggcg caggtctgga
     3721 accacacttt cttctggaac tgcctgacgc caaacggtgg cggcgcgccg ggcggtgaac
     3781 tggctgcggc catcgatggc aaatggggat cgtttgcgga ttttgccaaa gccttccagg
     3841 cttccgcggt cggcaatttc ggctccggct ggacctggct ggtcaaaaag gccgatggtt
     3901 cgctggacat cgtcaacatg ggtgccgccg gcaccccgct gaccaccggc gacaaggcgc
     3961 tgctgtgcat cgacgtctgg gagcacgcct actacatcga ttatcgcaac ctgcgtccga
     4021 agttcgtcga aaccttcctc ggcagcctcg ccaactggcg tttcgccgaa cagaactttg
     4081 ccgcatagca aaccgtcgac gcccgctcgt gcgggcgtca gaagctaccc cagtccgggt
     4141 cgggcaacgc ggtgaatgcc tgccgcagac gctcgcccca gagcgtgcgg atggtgttga
     4201 aataggcgtt ttgcgggcca atactgacat gatcgcgcac ccgcagggca tcgcgctcgt
     4261 agatcagcaa atcgagcggc atgcccaccg agaggttcga gcggatcgtc gaatccatcg
     4321 aaatcagcgc gcacttggcc gcttcctcga aggtggtacg gcggttcacg acgcggtcga
     4381 tgatcggctt gccgtatttt gattcgccga tctggaaata cggggtgtct tcgctggctt
     4441 cgatgaagtt gccggctgca tagatgttga acaggcgcgg atcttcttcg ccgatctgac
     4501 cgccaaagat gatcgacgcg ttgaactcga taccgaattc ctgcagtgcc gcggcatccc
     4561 gctgatgcac ctcgcgcacg acgctgccga catggcgcgc ggcttcgaag aggttgggca
     4621 gactgcagag attctcctgg tcgctgcgat ccagacgttc gcggagaata ttgaccaccg
     4681 attgactgac cgaaaggttg ccggcggtca tcaggaccag cacgcgctca ccgacgcgct
     4741 cgatcaccgt catcttgcgg aaggtgttga tgtggtcgac gccggcgttg gttcgcgagt
     4801 cggacagaaa caccaggccg gcatccagcc gcatggcgac acagtaggtc atgaacgacg
     4861 gccacgcggg cgacgcgccg tagcggcagg cacgctgggc gaggccacgg cgcgctcagc
     4921 caggaatcag gaaatcgcga ctgataccat cgccaagttc gtagatacga tccatgaagt
     4981 cggtcaggta ctcgtgtagt ccaccggcca gcacatcgtc gatacgcgcg aaatgcagcc
     5041 tggcatgcag cacacctgcc tgacgttcgg tttccgcgga agcgccattg gcaatcaact
     5101 gcagcatccg gacgatgccg tcggcacagg catgcagcga cctcggcatg tccatgcgca
     5161 ggatcagcaa ctcggcgacc cggccaggcg tgatggcgtc gcggtacacc ttgcggtata
     5221 cctgaaacgc cgacagcgag cgcagcagcg ccccccagcg atagaagtcg ctggccccct
     5281 cgtcgtcatt ctggcgcagg atatggtact gcatgtcgag aatgcgggcg gtgttgtcac
     5341 cgcgctcaag caggccgcca aggcggatga aatgcagtgc atcatccttg agcatggtcc
     5401 cgaaggtcac cccgcgggtg accgccgaac gcagcttgac ccagtcgaag aattcgctga
     5461 ccccgcgcgc gaggatgtgc tcgaagttct gctgacgcag ttcgatccac gtggcgttga
     5521 cgctttccca catctcggcg gtcaacgtgc cacgcacggc gtgcgcattt tcgcgcgcgg
     5581 cccgcaggca actgtagatc gacgagggat tggacgggtc ggaaaccatg aagcgcagga
     5641 cgttctcgac attcacctcg ttgtagacca gcgcaaaagc agcctcgagg ctgttgaggg
     5701 caagaatggc gcgccagttc tcgttctgcg aggcaacgga ctgcggcacc agtgacatct
     5761 ggtaggacac gtcgagaaga cgtgcgaggt tctcggcgcg ctcggtgtaa cgcgccatcc
     5821 agtacaggtg atcggctgtg cggctcaaca tctcggctct ccgtgcatct cagttttcca
     5881 acacccaggt gtccttggtg cccccgccct gcgacgagtt cacgaccagc gagccttcgc
     5941 gcagcgccac gcgcgtcaga ccgcccggca ccatcccgat gccacgcttt ccggacagga
     6001 caaaaggacg caggtcaagg tgacgcgggg cgatgcccgc gtcgacgaaa gtaggacagt
     6061 tggacagcgc cagcgtcggc tgcgcaatgt acttctcggg ggcggcgagg atacgggcgc
     6121 gaaaatcctc gatctcggcg tgcgtcgagg ccggcccgac cagcatgccg tagccaccgg
     6181 cgccatgcac ctccttgacc accagttccg gcaggtgtgc caggacgtat ttgagatcgt
     6241 ccggtttgcg cagcatccag gtcggaacgt tgttgagctt cggctcttcg ccgaggtaga
     6301 agcggatcat ctccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga
     6421 cgcccatctg ctgcgccagg aaggtatgtt cgaaataggc gctgttgtag gcccccggcg
     6481 tcagcaacac gaccgttggg tcggcaaccc ctttcggcgc cacggtgcgc aggttttcga
     6541 ggagcatgtc tggataatgc tgaaccgggg cgattagatg ccgcgagaac agttccggaa
     6601 acagccgcat catcatcttg cggtcttcga tcatgtacga gacgcctgaa ggcacgcgca
     6661 ggttgtcctc gaggacgtag gactcgccgg cgccggcacg cacgatgtcg acaccggcga
     6721 tatgggcgta gatgcccgac ggcacgtcga cgcccatcat ctcgcgacga aactgcgcat
     6781 tgttcaggac ctgctcggac ggaatgacgc cggcccggag gattgcctgc tcgtggtaga
     6841 tgtcgtgcag gaaggcgttg agcgccttca cccgctgcca taaaccggcc tgcagcgcct
     6901 gccactcggc tgccgggata atgcgcggga tgatatcgaa cggaatcagt cgttcggtac
     6961 cggactcctc gccataaacg gcgaaagtga taccgacgcg gtggaaagcc gtgtcagcct
     7021 cggcgcgctt gcgctccagg cgttccggcg gcatccctgc cagccattcc gcaaaggcgc
     7081 tgtagtgtgg ccgcagggtc ccggccgaat cgtacatctc gttgtagaag tctgcgttca
     7141 tgatgctcac caccaggttg ggtctgcaac taaaaagcat gaatcgtgcc aaataaaaac
     7201 cgtcggaagg tcgggaaagc gggcaccgaa taagccatgc tatgccagaa tatggtgcgg
     7261 ggccagcgct ttacgccccc tactggtgca tccgcggccc acactgcgtc agcgatgcgc
     7321 cgccgccgat agatcacgtc gatttactcc agtataaagc tgccgtgggc gcgcgatctt
     7381 gtattccgaa tcggccagca tttcctccca ttgcgccatc cagccggcgg tgcgggcgag
     7441 agcctggatg caggcgaaca tcgatgtcgg aatacccagc gccttctgca cgatggcgga
     7501 atagaaatcg acactcggat agagaccctc agcgacgaaa tacgggtcct ccagcgacaa
     7561 tgtctctaac gcctttgcca gcgcgaacag acgatgctgc tccagcccca gttcctgcag
     7621 gacctcgtcg cagacttccc gcagcaactg cgcacgcggg tcgaagtgcc ggtacaccgg
     7681 gtgcccgaaa ccgctgaacg tgaagccatt gcgcttgttg cgggcacggg cgacgaatcc
     7741 gccgaggcgc gacgtatcgc cgatctcctg gagcatggcc aggcacgcct cgctcgctcc
     7801 actatgcgcg ggccctgaca gacaggcgat ggccgccgac acacaggcaa agggattggc
     7861 gcccgaggaa ccggccatcc gaaccgtcga ggtcgaagca tcctgcccct ggtcggcgaa
     7921 gacaatgaac aacctgtcga gcgcacgcac cagcaccgga ttcggccgaa actcctcacg
     7981 cggggtagcg aacatcatgt gcaggaagtt ggcggcatac ccgagatcag cacgtggatt
     8041 catgaacggt tcgccgatcg agtacttgta ggccatggcg acgatcgtcg gcatcctggc
     8101 aacgatccgg tggaaattga ggttgcgttg cgcagtatcg aaatagtctg ccgcgtcgtg
     8161 attaaacgcc gacaacgcac ccaccacccc gaccatcacg gccatgggat cggcgtcacg
     8221 gcgaaaaccc tgatagaact tcaccatctg ctcgtgcacc gacgtgtgct ttctgatcgc
     8281 ggactcgaac gcaaccttct gcccggcgtc agccaattcc ccgtgcttca gcaggtaggc
     8341 cacatcaagg aagtcacatt gttcggcgag ttgtttgatc gggtagccgt gatggagcag
     8401 ctctcccttc tcgccatcga caaaggtgat tttcgacttg caactggcgg tcgactcgaa
     8461 acctgaatcg tacgcgaagc gaccggtcct ggcgcccaac gtgcgaatat ccaggcagtc
     8521 gctaccgtgt gtcggcgcca ggactgaaaa atgcactgcc ggctcgccaa ctatgctcaa
     8581 ggtggctttg cgctcgctga ccatgacttc ctccttttca gattcctcga cgaccgcgct
     8641 tgcaaacggc ggacgctcag cccggcaaca agccggtcac ccgcaccctt ggcgccgccg
     8701 ccctcttttg gggcttcgtc ggcgccttgc gcaccgacgc tgtgcccccg cgccctgcga
     8761 atagaagttt tctgcccgga ccggcctggc ccggaggccg caaagcgctc tacgacaaac
     8821 gctcagcgcg cacggcaccc cgttctgcaa gccaaacaaa gcaaaataca ttcccgcatg
     8881 caacccgaat ttggtacgga atttgcttat ttcacggcag acgccccgga gcggctcatc
     8941 atctgggtcc cgcacggcgg cgtgccgaca cggccggcaa gcacatgtcc aaccgcccgt
     9001 gcaggcggcg ttgaaaaggt tgaagatcac atgaatccac cgatggtact cccctggctc
     9061 gcaagcaagg ctggcgtgag cattcgccaa gccgaagcgc tctggccgtc agcgtgtcgt
     9121 caagccgcgc aaatcactgg ccagcgggac ggctccacct actggggcac ggcggtgcaa
     9181 accctgttgc gcctgctgca ggaggagcgc aggcagacgg tttccctgct gacctggccg
     9241 tggctactct tgcagagtgg cgtggaacac tggtcccggc tcaccaggaa ctggctggcg
     9301 ccgatcgatg cttcgttgca cacctggaaa ctccacctcg aaggtagtcg gcac
//
LOCUS       DAOSIT010000021         9080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7253_length_9080_cov_10.101828, whole genome shotgun sequence.
ACCESSION   DAOSIT010000021 DAOSIT010000000
VERSION     DAOSIT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7253_length_9080_cov_10.101828"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..2454
                     /gene="parC"
                     /locus_tag="PLL14_01145"
     CDS             97..2454
                     /gene="parC"
                     /locus_tag="PLL14_01145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOG02550.1"
                     /translation="MRKDSTPDLFADLPVDGQSPPPAPPAPPAQPAVEDDDSILLHDS
                     AARDYLEYAIAVVKGRALPDVKDGLKPVQRRVLFAMRELGLSATAKPVKSARVVGDVI
                     GKYHPHGDTSAYDAMVRVAQPFMLRYPLVDGQGNFGSRDGDNAAAMRYTEARLTPIAE
                     LLLGELGEGTVDFQPNYDGSFEEPAFLPARLPFLLLNGASGIAVGMATEMPSHNLREV
                     AAAAMHMIEHPQAGVGELMQHVPGPDYPGGGQIISAPGDIAAAYGSGRGSLRVRARYE
                     IEEMARGAWQVVFKELPPGVSSAKVLSEIGALLNPQPKAGKKAIEQPAAQLKALMASQ
                     LDRARDESGKDDDVRLVFEPSTSRIDRNEFVALLLAHTSLETSAPINLVAVGIDGRPC
                     QKSLAELIGEWCAFRIRTVHRRTEYRLQKADDRIHILEGRHIVFLNIDEVIRIIRESD
                     DPKAALMARFTLSDRQAEDILEIRLRQLARLEGIRIEQELAKLRGEREGLLKLLGSDA
                     LLRRQVIREIRADAEKHGDVRRTIIQPAAIASRSEVAAVADEPVTVIISEKGWARVRQ
                     GHGLDLSGVVFKDGDRAGSAQECRSVDSLVILSTEGRAFTVAVASLPDGRGMGAPLSS
                     FVDLGTGKIAHVLTGLPEEQLLVAKTSGYGFVCTYADLLSRQKAGKAFLSVEDGATIL
                     PPVRLAGKDHLAALSEDGRLLIFPLEQMKRLSAGKGVQLIALREGESLRAVVATLGAR
                     VTIKGTVRNRIKTLLSEERHCGLRARRGSPVGQLGNVMLEACPEP"
     gene            2561..3004
                     /locus_tag="PLL14_01150"
     CDS             2561..3004
                     /locus_tag="PLL14_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOG02551.1"
                     /translation="MCGVLVLCSTLVLAEQVGCVTTEWKLVGANHRVCVESFPDPKIP
                     GITCHVSQARTGGVSGSFGLAQDPSQFSLACRQTGPITLPAKLPKEATVFAEDTSILF
                     KETRVVRMWDEAHKTLVYLAISRKLVNGAPANSISTVPVMPWGAP"
     gene            3128..4492
                     /locus_tag="PLL14_01155"
     CDS             3128..4492
                     /locus_tag="PLL14_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG02552.1"
                     /translation="MAVSEVRRPDCDVSRLGQVFTPPMIVDAMLALVRNCGRVLEPAC
                     GDGAFLQHFPFALGIEIDARHAPAGARVMDFFELPERETFATIIGNPPYVRYQDIQPE
                     TRRHVRRSVLDGRANLYLFFIEKCLRHLAPGGELVFITPRDFVKATSAVPLNRLLHAA
                     GTITDFVDLGDSRLFDGAAPNCAIWRFERDNFLRLTRHAQQGVADGLAGLARLQWEER
                     HFVESGGHLLFTRGDYALQLADIAFVKVGAVSGADDLYVSEDHGNRSFVCSATVATGN
                     TRRMLWCAPGDPPPAMLLSHRKRLIARRIRPFDDSNWWQWGRGYHQTEQPRVYVNNKT
                     RRSQPFFVHPCTHYDGSVLAIFAHDPTVDVQALAAALNAVDWSDLGFVCDGRYLFTQR
                     SLEQTPLPESFRAFLPGGGVRSQPADASLLPPLQVLPPSISRPRPVPKGRRKAHGSTP
                     HDGL"
     gene            4464..5774
                     /gene="gshA"
                     /locus_tag="PLL14_01160"
     CDS             4464..5774
                     /gene="gshA"
                     /locus_tag="PLL14_01160"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOG02553.1"
                     /translation="MVPRLTTAFSGPLLEIERCVLDAMPEIERWLRGQWQEHTPPFYG
                     SVDLRNAGFKLAPVDMNLFPGGFNNLDATFLPLCVQAVVTAVDRICPDARRLLLIPEN
                     HTRNVFYLQNVAQIAAILRLTGLEVRLGSLLPEIDKPTPLTLPDGTTLLLEPVLRNRR
                     RIGLQDFDPCAILLNNDLSAGIPAILQHLDEQFVLPPLHAGWAVRRKSNHFAAYDEVA
                     GDFARLVGIDPWRINPYFSVCDSVNFHDRQGEECLAANVDAVLGLIREKYQEYAIDET
                     PYVVVKADAGTYGMGVMTVKDASQVTGLNRRQRNKMSVIKEGLAVSQVIIQEGVHSYE
                     RVGSGKDEGVAEPVVYMIDRFVVGGFYRVHSGRGKDENLNAPGMHFEPLAFETSCSLP
                     DQCQNPDAAPNRFYAYGVVARLAQLAASLELEHTAPVEEALACA"
     gene            5765..6718
                     /gene="gshB"
                     /locus_tag="PLL14_01165"
     CDS             5765..6718
                     /gene="gshB"
                     /locus_tag="PLL14_01165"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOG02554.1"
                     /translation="MRIAFVVDPLPSLRAYKDTSIALMRAAQASGHAVWTIQQEAIHW
                     SPLHGVCAEAVHLEMLADDKDWFVEVDRHVVRLRDFRAVLMRKDPPFDVEYVTTTWLL
                     ERAEAEGARVFNRPRALRDHSEKLSVAEFAQFSVPTLVARDPLLIHAFVDLERDTVLK
                     PLDGMGGSEVFRVSHDDPNRNVIVETLTRHGTRTIMAQRYIPEIVDGDKRILIVGGEV
                     VPYCLARIPKAGETRGNLAAGGTGVARELSARDREIASTLAPVLAARGLLLVGLDLIG
                     DWLTEINVTSPTCMVEIAEQTGFDAAEMLIAALERQCAARA"
     gene            6700..7737
                     /locus_tag="PLL14_01170"
     CDS             6700..7737
                     /locus_tag="PLL14_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG02555.1"
                     /translation="MRGARLVLLLVVFGCLVACGKAPLHHQEAFVFGTRVEVLIAGLD
                     EAQARPAAAAVLREFDRLHRSYHAWKPSELSALNAAIAAGKRQAITPEMAALIVEAQR
                     LTRLGEQLFDPGIGRLVSLWGFQSDELPATLPDAGALAAWRVARPSILDLRIDDHYAS
                     SRQPQLALDFGGYLKGVALDRAASILRAQGVRDALINIGGNVMALGSKNGQSWRVGIQ
                     HPRQPGPLATLELQDGEAIGTSGDYQRYFEVDGRRYCHLLDPRSGQPVTHTQALSVLI
                     TPRANTGALSDVASKPLFIAGDDWPRLARALAVEHVLRVDAEGRLQVSSAMQRRLVFV
                     GGAPKALQVMP"
     gene            7808..8200
                     /locus_tag="PLL14_01175"
     CDS             7808..8200
                     /locus_tag="PLL14_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HOG02556.1"
                     /translation="MIGILLITHGSYGEALIQNVCHVLNRRPALIAQFGVAAQDDPLD
                     ILPLARLLLKEVDGGEGVLVMTDVFGATPGNLALKLLEPGHVEGVAGLSLPMLLRALT
                     YRERGMETMLQKAISGAHDGITKLPPPA"
     gene            8223..8492
                     /locus_tag="PLL14_01180"
     CDS             8223..8492
                     /locus_tag="PLL14_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG02557.1"
                     /translation="MVRAETAILNKLGLHARASAKLTQLASHFASDVWMEKGARRINA
                     KSIMGVMMLAAGKGSTVIVETDGADEQAASAAILELIAGRFGETE"
     gene            8500..>9080
                     /locus_tag="PLL14_01185"
     CDS             8500..>9080
                     /locus_tag="PLL14_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOG02558.1"
                     /translation="MSFTLHGLAVSGGIAIGQAHLMSHATLEVSHLFIAPRLVDKEVA
                     RFEAALVRVREEFANLKGRMQHASAEFGAFIDLHSMILADPELAEAPKQLIRERRCNA
                     EWALVQQMEVLVDQFERFDDQYLRERSYDVRQVVERVIKELVGQPGRMALRTGKGIKE
                     ENLIVVAHDLSPADVIAYKEHHFAAFVTDVGGST"
BASE COUNT         1589 a         2773 c         3017 g         1701 t
ORIGIN      
        1 acatgttgat gggcaagaac gagtccgccg gccggcgggc gtggatggaa gaaaatggcg
       61 ccctggtcgg tgcggagaca tgaaggtgag cagcaagtga ggaaagattc gacccccgac
      121 ctgtttgccg acctgcctgt cgatggccag tcgccaccac cggcaccccc agcgcctcca
      181 gcgcagccgg ccgtggaaga cgatgacagc attctgctgc acgactcggc ggcgcgcgac
      241 tacctggaat acgccatagc ggtggtcaag ggtcgggcgc tgccggatgt caaggacgga
      301 ctgaaaccgg tgcagcgtcg ggtactgttc gcgatgcgtg aactggggct gtcggcgacc
      361 gccaagccgg tgaagtcggc gcgcgtcgtc ggcgacgtga tcggcaagta ccacccacac
      421 ggcgacactt cggcctatga cgcgatggta cgggtggcgc agccgttcat gctgcgctat
      481 ccgctggtcg atgggcaggg gaatttcggc tcgcgtgacg gcgacaacgc ggcggcgatg
      541 cgctacacgg aagcgcgcct gacaccgatt gccgaactgt tgctcggcga actgggtgaa
      601 ggcaccgtcg attttcagcc gaattacgac ggttccttcg aggaaccagc cttcttgccg
      661 gcgcggctgc cgttcctgtt gctcaacggc gcttctggaa ttgccgtggg catggccacc
      721 gaaatgccct cgcataacct gcgcgaggtc gccgctgcgg cgatgcacat gatcgagcat
      781 ccgcaagccg gcgtcggcga gttgatgcag catgttccgg ggccggatta tccgggggga
      841 gggcagatca tctctgcgcc cggcgatatc gccgccgcgt atggcagtgg ccgcggtagc
      901 ctgcgcgtgc gtgcccgcta cgagatcgag gagatggcgc gcggtgcctg gcaggtggtg
      961 ttcaaggagt tgccgccggg cgtttcgtcg gccaaggtgc tgtccgaaat cggagcgttg
     1021 ctcaacccgc agcccaaggc cggcaagaag gcgatcgagc agccggcggc gcagctcaag
     1081 gctctgatgg cctcgcagct cgatcgggcg cgcgacgagt cgggcaagga cgacgatgtg
     1141 cgactggtct tcgagccgag cacctcgcgc atcgaccgca acgagtttgt ggctctgctg
     1201 ctggcccata ccagcctcga aacctcggcg ccgatcaatc tggtcgcggt cggcatcgac
     1261 ggccggcctt gccagaaatc gctggccgag ttgatcggcg agtggtgcgc tttccgcatc
     1321 cggaccgtac accggcgtac cgaatatcgc ctgcagaagg ccgatgaccg catccacatt
     1381 ctggaaggcc ggcacatcgt cttcctgaac atcgacgaag tgatccgcat catccgcgaa
     1441 tcggatgacc cgaaagcggc gttgatggcg cgcttcacac tgtctgatcg acaggcggaa
     1501 gacatcctcg aaatccgcct gcgtcaactg gcccggctcg aagggatcag gatcgagcag
     1561 gaactggcga aactgcgcgg cgagcgcgaa ggactgctca agctcctggg tagcgacgcg
     1621 ctgctgcgcc ggcaggtgat ccgcgaaatc agggccgatg ccgagaagca cggcgacgtg
     1681 cggcgtacga tcatccagcc ggcggccatc gcgtcgcgct cggaggttgc cgccgtcgcc
     1741 gacgagccgg tcacggtgat catttccgaa aagggctggg cgcgtgtcag gcaggggcac
     1801 ggcctggacc tctcgggtgt tgtcttcaag gatggtgacc gcgccggttc ggcgcaggag
     1861 tgtcggtccg tcgattcgct ggtcatcctc tcgactgaag gccgggcgtt taccgtggcg
     1921 gtcgccagcc tgccggacgg tcgcggcatg ggtgcgccgc tgtcatcgtt cgtcgacctc
     1981 ggcaccggga aaattgcgca cgtgctgacc ggcttgccgg aggagcaatt gctggttgcc
     2041 aagacctccg gttacggctt tgtctgtacc tacgccgacc tgctttcgcg tcagaaggcg
     2101 ggcaaggctt ttctgagcgt cgaagatggc gcgaccatcc tgccgccggt gaggcttgcg
     2161 ggcaaggatc atctggcggc attatcggag gacggtcgcc tgttgatctt ccccctggaa
     2221 cagatgaaac gcctgtcggc cggcaagggg gtccaactca tcgcactgcg cgaaggtgaa
     2281 tcgctgcgtg ccgtggtggc caccctgggc gcgcgggtga cgatcaaggg tacggtccgc
     2341 aaccggatca agacgctgct ttccgaagag cggcattgcg gtctgcgcgc ccgccgtggc
     2401 tcaccggttg gacaactcgg caacgtgatg ctggaagcct gtcccgagcc ctgattgttg
     2461 gcaggctgtg ccggggcgtg gcggttaacc tcctggtgct gcaaaggaga aagaatttgt
     2521 gaagagcaag acgagcacga gtgggtacag acaggccggc gtgtgcgggg tgctggtcct
     2581 gtgcagcacg ctggtgctgg ctgagcaggt cggctgtgtg accaccgagt ggaagctggt
     2641 gggggccaat caccgggttt gcgtcgaaag tttccccgac cccaaaatac cgggtatcac
     2701 ctgccatgtc agtcaggcca ggaccggtgg cgtcagcggc tcgttcgggc tggcgcagga
     2761 cccgtcgcaa ttctcgctgg cctgcaggca gaccggaccg atcacgctgc ctgcgaagct
     2821 ccccaaggag gcgacggtgt ttgccgaaga cacctcgatt ctgttcaagg agacccgcgt
     2881 tgtccggatg tgggacgaag cccacaagac actggtctat ctcgcgatca gccgcaagct
     2941 tgtcaatggc gcgccggcaa acagcatttc gacggtaccg gtgatgccct ggggggcgcc
     3001 ttgatgtgct gccgaccaac gactaccgtc ccgatccgga aggtcctgat ggttcaggaa
     3061 ggcctccagt gagccgccca ggctggccgt cgttttctcc tccaggcgat cgcgcggctg
     3121 cccggcgatg gcggtgagcg aggtacgacg acccgattgc gacgtcagtc gactcgggca
     3181 ggtgtttacg ccaccgatga tcgtcgacgc gatgctggct ctggttcgca actgcgggcg
     3241 ggtgcttgag ccggcctgtg gtgacggcgc tttcctgcag cattttccgt ttgcgctggg
     3301 catcgagatc gacgcacgcc acgcacccgc cggggcgcgg gtgatggact tcttcgaact
     3361 gccggagcgc gagacatttg ccaccatcat cggcaatcct ccgtatgtgc gttaccagga
     3421 tatccagccc gagacgcgcc ggcatgtccg gcgcagcgtc ctcgatgggc gcgccaacct
     3481 gtatctgttc ttcatcgaga agtgcctgag gcatctggca ccgggcggcg agctggtgtt
     3541 catcacgccg cgagatttcg tcaaggccac ttcggcggtc cccctcaacc ggctgctgca
     3601 cgccgcggga acgatcaccg atttcgtcga tctcggggat tcacggctat tcgacggggc
     3661 ggcgccgaac tgcgcgatct ggcgtttcga acgcgacaac ttcttgcgtc tgacgcgcca
     3721 tgcgcagcag ggcgtggccg acggtctggc gggtctggcc cggctgcagt gggaggagcg
     3781 gcattttgtc gagagtggcg ggcacctgct cttcacgcgc ggcgactatg ccctgcagct
     3841 cgccgatatt gccttcgtca aggtgggggc agtctctggt gctgacgatc tctatgtcag
     3901 cgaagatcat ggtaaccgca gcttcgtctg ttcggcgact gtggccaccg gtaacacacg
     3961 gcgcatgctc tggtgtgcgc cgggcgatcc gccgccagcg atgctgctgt cgcacagaaa
     4021 gcgtctgatc gcgcgtcgta tccggccatt cgacgactcc aactggtggc agtgggggcg
     4081 tggctaccat cagacggaac agccgcgcgt gtatgtcaac aacaagacgc gccggtcgca
     4141 gcccttcttc gtacacccct gcacgcatta cgacgggtcg gtgctggcga tttttgccca
     4201 tgacccgacg gtcgatgtgc aggcactggc tgccgcgctg aatgcggtcg attggtccga
     4261 cctcggcttt gtctgtgacg gtcgatacct gtttacacaa cgtagtcttg agcagacacc
     4321 gcttcccgag tcgttccgtg ccttcctgcc cggtggcggt gtaagatcac agccggcgga
     4381 tgcgtctctc ttgccgccgc tgcaagtttt gccaccgagc atttcccggc cccggccggt
     4441 gccgaaggga aggagaaaag cccatggttc cacgcctcac gacggccttt agcggtccgc
     4501 tgctggagat cgaacgttgt gtcctcgatg ccatgccgga aatcgagcgc tggttgcgcg
     4561 gtcaatggca ggaacatacg ccgccctttt acggttccgt cgatctgcgc aatgccggtt
     4621 tcaagctggc gccggtggac atgaatctct ttcccggggg attcaacaat cttgacgcca
     4681 cgttcctgcc gctctgcgtc caggcggtgg tgacggccgt cgaccgcatc tgccccgacg
     4741 ccaggaggtt gctgctgatt cccgaaaacc atacgcgcaa cgtcttttat ttgcagaatg
     4801 tggcccagat cgcggcaatc ctgcgcttga ccggtcttga ggtgcgcctg ggctcgctgc
     4861 tgccggaaat cgacaagccg accccgctga ccttgcccga cggtacgaca ctgcttctcg
     4921 aaccggtgct gcgcaatcgc cggcggattg gcctgcagga tttcgatccc tgcgcgatcc
     4981 tgctcaacaa cgatctgtcg gcaggtatcc ctgcaattct gcagcatctc gacgaacagt
     5041 tcgtgttgcc gccgttgcac gccggttggg cggtgcgccg caaatccaac cactttgctg
     5101 cttacgacga ggtcgcgggt gactttgcca ggctcgtcgg catcgacccg tggcggatca
     5161 atccctattt ttcggtctgt gacagtgtca acttccatga tcggcagggt gaggagtgcc
     5221 tggcggccaa tgtcgatgcc gttctcggcc tgatccgcga gaaataccag gaatacgcga
     5281 tcgacgagac gccctacgtg gtggtcaagg ccgacgccgg tacctatggc atgggagtga
     5341 tgacggtcaa ggatgcgtcc caggtgaccg gtctcaatcg ccgacagcgc aacaagatgt
     5401 cggtgatcaa ggaaggtctg gcggtttcgc aggtgatcat ccaggaaggc gttcatagct
     5461 acgagcgggt gggcagcggc aaggacgagg gggtcgccga acccgtcgtc tacatgatcg
     5521 accgctttgt cgtcggcggc ttctatcgcg tgcacagcgg gcgcggcaag gacgaaaacc
     5581 tcaacgcgcc gggcatgcac ttcgagccac tggctttcga gaccagttgc tcgttaccgg
     5641 atcagtgcca gaatcccgac gccgcaccca accgcttcta cgcctatggg gtcgtggccc
     5701 gcctggcaca actggccgcg tcgcttgaac tggaacatac ggcacctgtt gaggaggcac
     5761 tggcatgcgc atagcttttg tcgtcgaccc gctgccgtca ctgcgggcgt acaaggacac
     5821 cagcatcgct ctgatgcggg ccgcgcaggc ttccgggcac gcggtgtgga cgatacagca
     5881 ggaggcgatt cactggtcgc cgctgcacgg cgtctgtgcc gaagcggtgc atctggaaat
     5941 gctggccgac gacaaggact ggttcgtcga ggtcgatcgc catgtcgtgc gcctgcggga
     6001 ttttcgggcc gtgctgatgc gcaaggaccc gcccttcgac gtggagtacg tgaccacgac
     6061 ctggctgctg gaacgtgccg aagccgaagg cgcgcgcgtc ttcaatcgac cgcgcgcctt
     6121 gcgtgaccat tccgaaaagc tgtcggtcgc cgagttcgcg caattttcgg tgccgacact
     6181 ggtagcccgt gaccctcttc tgatccatgc gttcgtcgac ctcgaacgcg atactgtcct
     6241 caagccgctc gacggcatgg gcggtagcga ggtcttccgc gtgtcgcacg acgacccgaa
     6301 ccgcaacgtg atcgttgaaa cgctgacccg ccacggcacg cgaacgatca tggcccaacg
     6361 ctacattccc gagatcgtcg atggcgacaa acggatcctg atcgtcggcg gtgaggtcgt
     6421 tccctattgt ctggctcgca ttcccaaggc cggggagacg cgcggcaacc tggctgccgg
     6481 tggcaccggc gtggcccgcg aactgtccgc tcgtgaccgc gagatcgcca gcacgctggc
     6541 gccggtgctg gccgcacgcg gtttgctgct ggtcggtctg gatctgatcg gcgactggct
     6601 gacggaaatc aacgtcacca gcccgacatg catggtcgag attgccgaac agaccggttt
     6661 cgacgccgcc gagatgctca tcgccgcgct ggagcgtcag tgcgcggcgc gcgcctagtc
     6721 cttctgcttg tggtgttcgg ctgccttgtc gcttgcggca aggcgccgct gcatcaccag
     6781 gaagcctttg ttttcggaac ccgcgtcgag gtgttgattg ccgggctgga cgaggcgcag
     6841 gcgcgtccag cagcggcggc cgtgctgcgc gagttcgatc gcctgcatcg cagctatcac
     6901 gcctggaagc cttccgaatt gagcgccctg aacgcggcca tcgctgccgg caagcggcag
     6961 gcgataacgc ccgaaatggc cgcgctgatc gtcgaagcac agcgcctgac gcggctcggc
     7021 gaacagctct tcgaccccgg gatcgggcgt ctggtcagcc tctggggctt tcagtccgac
     7081 gaactgccag caaccctgcc cgacgccggc gcgctggctg cctggcgagt ggccaggccg
     7141 agcatcctcg atttgcgcat tgacgaccat tacgccagta gccggcaacc acaactggcg
     7201 ctcgatttcg gcggctatct caagggcgtc gcgctggacc gcgcggcgag catcctcagg
     7261 gcgcagggtg tgcgtgacgc gttgatcaac atcggcggca acgtcatggc cctgggcagc
     7321 aagaacgggc aaagctggcg agtcggcatc cagcatccgc gccagcccgg tccgctggcg
     7381 acgctggaac tccaggacgg cgaggcgatc ggtacctcgg gggactacca gcgctacttt
     7441 gaagtcgatg gccggcgcta ttgccacctg ctcgacccgc gtagcggcca gccggtgacg
     7501 cacacgcagg cactgagcgt gttgatcacc ccgcgggcga acacgggggc gctttcagac
     7561 gttgcttcga aaccgttgtt catcgccggc gacgactggc cacgtctggc gcgtgcgctg
     7621 gcggtcgagc atgtgctgcg ggtcgatgcc gaaggtcgtc tgcaggtcag cagtgccatg
     7681 cagcgacgac tcgttttcgt cggcggtgcg ccgaaggcgc tccaggtcat gccgtgaagc
     7741 gttcgtcaat gctgccgagc ggcgcggaat cgcatagaat ggcggcctgt cgcaagggat
     7801 gaaggcaatg atcggtattc tcctgataac gcacggctcg tacggcgaag ccctgatcca
     7861 gaacgtgtgt catgtgctca acaggcgtcc ggcactgatc gcccagttcg gggtcgccgc
     7921 tcaggacgac ccgctcgaca tcctgccgct ggcgcggctg ttgctcaagg aggtcgacgg
     7981 cggtgaaggg gtgctggtaa tgaccgatgt gtttggcgcg acgccgggca acctggcgct
     8041 caagctgctc gaaccgggcc atgttgaagg cgttgccggt ctcagcctgc ccatgttgtt
     8101 gcgcgccctg acctatcgcg agagagggat ggaaacgatg ctgcagaagg cgatcagcgg
     8161 tgcacacgat ggcataacca agctgccgcc gcctgcctga gatcataata cgggagttca
     8221 ggatggttcg cgctgaaact gcaatcctca acaagctggg cctgcacgct cgtgcttcgg
     8281 ccaaactgac gcaacttgcc agccacttcg ccagcgacgt ctggatggag aagggggccc
     8341 gccgaatcaa tgcgaaaagc atcatggggg tgatgatgct ggctgcaggc aaaggctcga
     8401 cggtgatcgt cgaaaccgat ggcgccgacg agcaggcggc cagcgcagcg atcctcgaac
     8461 tgatcgccgg ccgcttcggc gagacggagt gagtccatca tgagctttac gctgcatggc
     8521 ctggcggtct ccgggggtat cgcgatcggt caggcgcatt tgatgtcgca cgccacgctc
     8581 gaggtgtcgc acctgttcat cgccccacgt ctggtggaca aggaggtggc ccgtttcgag
     8641 gccgcgctgg tgcgcgtccg cgaagagttc gcgaacctga aggggcgcat gcagcatgcg
     8701 tcggccgagt tcggtgcgtt catcgacctg cattcgatga ttctcgccga tccggaattg
     8761 gcggaggcgc caaagcagtt gattcgcgag cgtcgttgca atgccgaatg ggcgctggtg
     8821 cagcagatgg aagtcctggt tgaccagttc gaacgttttg acgaccagta cctgcgtgag
     8881 cgcagctacg atgtgcggca ggtcgtcgag cgggtgatca aggagcttgt tggacaaccg
     8941 ggccggatgg cgctcagaac gggcaagggg atcaaggaag agaacctgat cgtcgttgcc
     9001 cacgatctct cgccggccga tgtcattgcc tacaaggagc atcatttcgc ggccttcgtt
     9061 accgatgtcg gcggctcgac
//
LOCUS       DAOSIT010000022         8780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7515_length_8780_cov_10.249398, whole genome shotgun sequence.
ACCESSION   DAOSIT010000022 DAOSIT010000000
VERSION     DAOSIT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_8780_cov_10.249398"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1422
                     /locus_tag="PLL14_01190"
     CDS             <1..>1422
                     /locus_tag="PLL14_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOG02559.1"
                     /translation="MPALAHLNRVLPGLQPLLTYRAADWPHDLKAGLSVAAVAIPVAI
                     AYAQLAGFSPSTGLYASILPMIVYALAGTSRQLIIGPDAATCAMIAAVVTPLAAGNSG
                     LYLSFTIALTLLTGIFCVAASFLRLGGLADFLARPILVGLLNGVSISIMIGQVGKVLG
                     SKPEATRFFRQVLEIPDLILKAHVPTVLLSIGSVVVMLVMPRISKRVPASLVTMLIAG
                     LAAYLLRLDQMGVALIGPVASGLPDLEWPDLPFHEIGDLAGSAAGLALVLFTSGMLTA
                     RSFAERNGYDLDVDREFAAFGMANIASALSQGFAVTGADSRTAVNDANGGRTQMVSIV
                     AAAAISLLLLLGTGSLAWLPISALGVVLLFASWSLLDMQVFRRYHQIERAALVLGAIT
                     MFGVLAFGSIKAIMLAVTLALLMFIRRVARPVCEELVNVPGRAGLYNHQLFPDAATID
                     GLLLLRFCGPLVFFNANHFRSEV"
     assembly_gap    1423..1522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1523..2238
                     /locus_tag="PLL14_01195"
     CDS             <1523..2238
                     /locus_tag="PLL14_01195"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOG02560.1"
                     /translation="RFFTRLPVPAWVGHSSEALERATRYFPAVGLLVGAIAASIFSVT
                     STLWPTTLAVLASMAGTLYLTGGFHEDGWSDMVDGFGGGWEKTQILAIMKDSRIGSFG
                     AMALVMLLLTKFCALVEVDPLAIPAALLGGHAVSRFAAATLLRILDYVRDEGKAKPLA
                     TRIGGGELIFAGGTALLPLLLLPLPEALCGLTLAALATLWLARMFHRQIGGYTGDCLG
                     ATQQLSEVAFYCGLLCSFS"
     gene            2223..2819
                     /locus_tag="PLL14_01200"
     CDS             2223..2819
                     /locus_tag="PLL14_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG02561.1"
                     /translation="MQLFLIRHPRPLLAAGICYGQLDVEAADPQPLAERLRGLLPAGT
                     PVVASPSRRARGLAEALQRRPRFDRRLLEIDFGAWEGQPWDQVDRQLLDAWAADVLHF
                     VPPGGESAAMLQTRAVDCVRSLRGPRCALVTHAGVIRAVLGHWLGLPVGEWSRLTLDF
                     GSITRLEIERAQNELPSGRRAERQDHAPAAGVLHYLNR"
     gene            complement(2850..3764)
                     /gene="xerD"
                     /locus_tag="PLL14_01205"
     CDS             complement(2850..3764)
                     /gene="xerD"
                     /locus_tag="PLL14_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOG02562.1"
                     /translation="MKAVEVDPADLAEIDAFCDTLWLEDGLAKASLDSYRSDLMQLAT
                     WLAEQQLGSLCSIDEAILLRFVAMLSQTLRASSQARHVSTLRRFYRQQLARGRRSNDP
                     TLQVAMPVKPSRLPKVISEGQVERLLAAPQVETTLGQRDRAMLETLYATGLRVSELVG
                     LKLHELNLDMGVVRIFGKGGKERLVPLGEEARDWLCRYLGDARLALLEGRQSDDLFVT
                     ARGSAMTRQAFWLLIKRYALPAGMDPARLSPHVLRHAFATHLLNHGADLRVVQMLLGH
                     SDISTTQIYTHVARERLKLLHAQHHPRG"
     gene            complement(3751..4227)
                     /locus_tag="PLL14_01210"
     CDS             complement(3751..4227)
                     /locus_tag="PLL14_01210"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG02563.1"
                     /translation="MSPPAEPPYQAVISAAGFSLGVCCDEEQIHGIAFLPPRPAVAAR
                     NALAAEAASQLAAYLADAEFVPALPLRPAGTPFQRGVWQHIAAIPTHQTRTYGEIARA
                     LHNAPRAVGQACGANPFPVIVPCHRVVATGGLGGFAGQRAGFLLDVKRWLLAHEGC"
     gene            complement(4254..4826)
                     /locus_tag="PLL14_01215"
     CDS             complement(4254..4826)
                     /locus_tag="PLL14_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOG02564.1"
                     /translation="MKHLLLAVAAALSFHLAAAVEVGGVKFDDKTRVGSGELLVNGAG
                     VRKKAVFKVYAMALYLPEKRTDAEAVLAARGAKRISLSLLRDLSPQQFVEALQEGMTS
                     NHSEAEMAGLKERLKQFSDSMLAAGEPKTGTVVLIDWLPENGTRLTVNGQVKGKDIAG
                     EDFYKALLKIWLGNKPVQDDLKQALLGKAS"
     gene            complement(4940..6196)
                     /locus_tag="PLL14_01220"
     CDS             complement(4940..6196)
                     /locus_tag="PLL14_01220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOG02565.1"
                     /translation="MNIEQYMQRVGRQAREASRLIARADSQAKNAALRAIAAAIRREC
                     GTLVAANREDLAAAHAAGLEPALLDRLTLSEQGVAAMAEGVEQVAALPDPVGEISDLK
                     FRPSGIQVGRMRVPLGVIGIIYEARPNVTADAAALCLKSGNASILRGGSEAIRCNQAI
                     AALVQEGLASAGLPATAVQVIETTDRAAVGHLIRMREFVDVIVPRGGKGLISRLLAEA
                     RVPMIQHLDGNCHVYLDADADPEKALQIVENAKTQRYGTCNTAESLLLARTVVPRLLP
                     VIAAMLTRKGVEIRGCPETLDRVREARPASEEDYASEFLAPIISVKVVDGLGEAIEHI
                     NAYGSHHTDAIVTENYTSAMRFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDKI
                     HARGPVGLEGLTSQKWIVLGNGEVRA"
     gene            6327..6566
                     /locus_tag="PLL14_01225"
     CDS             6327..6566
                     /locus_tag="PLL14_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02566.1"
                     /translation="MILPAEVKVCATCSYWDGERTVDPELRMVVVAEDCAGECLVQTK
                     CSHGLNDVRAETEQCAWEHLAPDVAPGDDGKGISA"
     gene            complement(6588..>7433)
                     /gene="holA"
                     /locus_tag="PLL14_01230"
     CDS             complement(6588..>7433)
                     /gene="holA"
                     /locus_tag="PLL14_01230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG02567.1"
                     /translation="AIAGFDWNGLYHAAGSLSLFGGRTLIDLRIPTGKPGREGGAALQ
                     EYCTRPSPDSLLLVSMIGVDWREEKSAWMSALATAGVVVKLPTPGLGELPAWLAGRLR
                     RQKQSASAEGLRFIAERVEGNLLAAHQEILKLGVLYPDGELSPQQIRDAVLNVARYDL
                     DGLREALLVGDVARLVRAIDGLQQEGEAPLLILWAMAEEVRALAQISTGLARQQPLDA
                     LLRDARIWGARQTLIRQALKRLDRARAELALAHAARIDRMIKGLASGDVWDEFRQLGL
                     RLAAA"
     assembly_gap    7434..7533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7534..7895)
                     /locus_tag="PLL14_01235"
     CDS             complement(<7534..7895)
                     /locus_tag="PLL14_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02568.1"
                     /translation="MRAALPSAWLAVVLALLAGCGFQLRGAYTLPFESLYLSMPEYSV
                     IGASLKRAIRASGSTRLALTASDAQATLLPGAEYRDRVILSLSGSGRISELRLRYLYN
                     YRLTDDKGRDLVPPATIEL"
     gene            complement(7936..>8780)
                     /locus_tag="PLL14_01240"
     CDS             complement(7936..>8780)
                     /locus_tag="PLL14_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG02569.1"
                     /translation="GKKQWLNPADVDAVHDERGRASGATLRADGLPVVVGGIEKMSKS
                     KNNGVDPQALIDQYGADTARLFIMFASPPDQSLEWSDAGVEGAFRFIKRLWRIVYEHV
                     SAGPVAAAPAADLSGELKALRRQLHQTIGKVADDYGRRKQFNTAIAAVMELLNAYGRI
                     ADDSPAARAVRQEALEAVTLMLSPIVPHVCEALYAALRPGHTASQEAFPVVDAGALVQ
                     DEIELVLQINGKLRGSLRVPAGADRAAIESAALACEVARKHLAGAAPRKLVVVPGRLV
                     NIVA"
BASE COUNT         1406 a         2909 c         2744 g         1521 t
ORIGIN      
        1 catgccagcc cttgcccatc tgaatcgcgt gctgcccgga ctgcaacccc tgctcaccta
       61 ccgcgcagcg gactggccgc acgacctgaa ggctggcctc tcggtggcgg cggtggccat
      121 cccggtggcc atcgcctacg cgcaactggc aggcttcagt ccttcgacag gcttgtacgc
      181 gtcgatcctg ccgatgatcg tgtacgcgct cgccggcacc tcgcggcaac tgatcatcgg
      241 ccccgacgcc gccacctgcg cgatgatcgc cgccgtggtc acgccactgg ccgcgggcaa
      301 cagcggcctg tacctctcgt tcaccatcgc cctgacgctg ctgaccggga tcttctgcgt
      361 ggccgcaagt ttcctgcgtc tcggtggtct ggccgacttc ctggccagac cgatcctggt
      421 cggcctgctc aacggcgtat cgatctcgat catgattggc caggtcggca aggtcctcgg
      481 cagcaaaccg gaagcgaccc gcttctttcg ccaggtgctg gaaattcccg atctgatcct
      541 gaaggcgcac gtgccgaccg tcctgctgtc gatcggcagc gtcgtggtga tgctggtgat
      601 gccgcggatc tcgaagcgcg tgccggcctc gctggtgacc atgctcatcg ccggtctggc
      661 cgcttatctg ctgcgcctgg accagatggg ggtggccctg atcgggccgg tcgccagtgg
      721 gctgccggac ctcgagtggc cggatctgcc gttccacgaa atcggcgatc tcgccggatc
      781 ggcggccgga ctggcgctgg tgctatttac cagcggcatg ctcaccgcgc gcagtttcgc
      841 cgaacgcaac ggctacgacc tcgacgtcga ccgtgaattt gccgctttcg gcatggccaa
      901 cattgcctcg gcgctgtcgc agggtttcgc cgtcaccggc gccgattcgc gcacggcagt
      961 gaatgacgcc aacggcgggc gcacccagat ggtcagcatc gtcgccgcgg cggccatttc
     1021 cctgctgctg ctgctcggaa ccggttcact ggcctggttg ccgatttccg cgcttggcgt
     1081 ggtgctgctc ttcgcgtcgt ggtcactgct cgacatgcag gttttcaggc gctatcacca
     1141 gatcgagcgc gccgccctgg tactcggcgc gatcaccatg ttcggcgtac tcgccttcgg
     1201 ctcgatcaag gcgatcatgc tggcggtgac gctggccctg ttgatgttca tccgccgggt
     1261 ggcgcgcccg gtctgcgagg agctggtcaa tgtccccggc cgggccggcc tctacaacca
     1321 tcaattgttt cccgatgcgg cgaccatcga tggcctgctg ctgctgcgct tctgcggtcc
     1381 gctggtgttc ttcaacgcca accatttccg cagcgaagtc cannnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nntgcgcttt ttcacacgct tgccggtgcc cgcctgggtc
     1561 gggcacagca gcgaggcgct ggagcgggcc acccgctatt ttccagcggt tggcctgctg
     1621 gtcggtgcga tcgcggcgtc gatcttcagc gtgacttcga ccctctggcc gacgacgctg
     1681 gcggtgctgg cgtcgatggc cggcacgctc tacctgaccg gcggatttca cgaggacggc
     1741 tggagcgaca tggtcgacgg tttcggcggc ggttgggaga agacgcagat cctggcaatc
     1801 atgaaggatt cgcgcatcgg cagcttcggc gccatggcgc tggtgatgct gctgctcacc
     1861 aaattctgcg ccctggtcga ggtcgatccg ctggccattc cggcggcgct gctgggcggg
     1921 cacgccgttt cccgcttcgc ggcggcaacc ctgctgcgca tcctcgacta cgtgcgcgac
     1981 gagggcaagg cgaaacccct ggccacacgc atcggcggcg gcgaattgat ctttgccggc
     2041 ggcacggcgt tgctgccgct gctgctgctt cccctgccag aggcgctgtg tggcctcact
     2101 ctggccgccc tggccacctt gtggctggcg cggatgtttc accggcagat cggcggctat
     2161 accggcgatt gtctcggtgc cacgcagcaa ctctcggaag tcgcctttta ctgcggactg
     2221 ctgtgcagct tttcctgatc cggcaccccc gcccgctgct ggcagccggc atctgttatg
     2281 gtcaactcga tgtcgaggcg gccgacccgc agcccctcgc cgagcgcctg cgtgggctgc
     2341 tgcccgccgg cacaccggtg gtcgccagtc cctcacggcg cgcgcgcggc ctcgccgagg
     2401 ccttgcagcg acgaccacgc ttcgatcggc gactgctcga aatcgatttc ggcgcatggg
     2461 aaggccagcc ctgggatcag gtcgaccgcc agctgctcga cgcctgggcg gctgatgtcc
     2521 tgcatttcgt cccgccgggc ggcgaatcgg cggcgatgct gcagacacgc gccgtcgact
     2581 gtgtgcggag cctgcgcgga ccacgttgcg cactggtcac gcacgccggt gtgatacgcg
     2641 ccgtgctcgg gcactggctg gggttgccgg ttggcgaatg gagccggctg acgctcgatt
     2701 tcggcagcat cacgcggctc gaaatcgagc gcgcgcagaa cgaactgccg tcagggcgaa
     2761 gggcggaacg gcaagaccac gcccctgcag cgggcgtgtt gcactatctc aaccgttgaa
     2821 accgggagcg tgccgccggc cgtcgaacct catccgcgcg ggtggtgttg ggcgtgcaga
     2881 agcttgaggc gttcgcgcgc gacatgcgtg tagatctgcg tcgtcgagat gtccgaatga
     2941 cccagcagca tctgcacgac gcgcagatcc gcgccatgat tgagcaggtg cgtggcaaag
     3001 gcgtgccgca gcacatgcgg cgacaggcgc gccggatcca tcccggccgg cagcgcgtag
     3061 cgcttgatca gtagccagaa cgcctgccgg gtcatcgccg agccacgcgc ggtgacgaac
     3121 aggtcgtcgc tctgccgccc ttcgagcaac gccagccggg cgtcgcccag atagcggcac
     3181 agccagtcac gcgcctcttc gccgagcggt accagccgct ccttgccgcc cttgccgaag
     3241 atccgcacga cgcccatgtc gagattgagc tcgtgcaatt tgagtcccac cagttcgctg
     3301 acgcgcaatc cggtcgcata gagggtttcc agcatcgccc gatcgcgctg cccgagtgtc
     3361 gtctcgacct gtggtgcagc gagcagacgt tccacctgtc cttcggaaat cactttcggc
     3421 aggcgcgaag gtttcaccgg catggcgacc tgcagcgtag gatcgttgct cctgcggccg
     3481 cgcgccaact gctgccgata gaaacggcgc agtgtcgaca catgccgagc ctgcgaggag
     3541 gcgcgcagcg tttgcgagag catggccacg aagcgcagca atattgcttc gtcgatgctg
     3601 cacaggctgc ccagctgctg ctccgcgagc caggtcgcga gctgcatcag atcgctgcga
     3661 taactgtcca gcgacgcctt ggccagcccg tcctcgagcc acagcgtgtc gcagaaggca
     3721 tcgatctcgg cgagatcagc cggatcgacc tcaacagcct tcatgcgcca gcaaccagcg
     3781 cttcacgtcg agcaggaaac ccgccctttg tccggcaaag cctccgaggc cgccagtcgc
     3841 cacgacgcga tggcagggaa cgatcaccgg aaaggggttc gcaccgcacg cctggccgac
     3901 tgcccgtggc gcgttgtgca gcgccctggc gatttctcca taagtgcgcg tctggtgcgt
     3961 cggaatcgcc gcgatgtgct gccagacgcc gcgttgaaag ggagttccgg cggggcgcag
     4021 gggcaaggcc gggacgaatt cagcgtcggc gaggtaggcc gccagctgac tcgcggcttc
     4081 ggcggcgagc gcgttgcgcg cagcgaccgc cggccgtggc gggaggaagg caatgccgtg
     4141 gatctgctcc tcgtcgcagc agacgccgag gctgaatcct gcggcactga tcaccgcctg
     4201 gtatggcggc tcggcgggcg ggctcatcgc tgcgcgcttc gcggcaccgc tcgtcaggag
     4261 gccttgccga gcagcgcctg cttcagatca tcctgcaccg gcttgttgcc gagccagatt
     4321 ttcagcagcg ccttgtagaa atcctcgccg gcgatgtcct tgcctttcac ctgcccgttg
     4381 accgtcagcc gggtgccgtt ctccggcagc cagtcgatga gcacgacggt accggtcttg
     4441 ggttcgccgg cggccagcat gctgtcggaa aactgcttca gacgctcctt caggccggcc
     4501 atttcagctt ccgaatgatt gctggtcatg ccttcctgca gcgcctcgac gaactgctgc
     4561 ggcgagaggt cgcgcagcag gctgagcgaa atccgcttgg cgcccctggc cgccagcacg
     4621 gcctcggcgt cggtgcgttt ctccggcagg taaagcgcca tcgcatagac cttgaagaca
     4681 gccttcttgc gtaccccggc accgttgacc agcaattcgc cggaacccac gcgggtcttg
     4741 tcgtcgaatt tgacgccgcc gacttcgacg gcagccgcga gatggaacga cagggcggca
     4801 gcgaccgcca gcaacagatg tttcatcgag tctcctttaa gggtgtgctt gcttcggcgg
     4861 gtctgggcaa acctgcagtg gcctccgcct gccactgctg tgctacggtc ggcaggtgct
     4921 gccgaccgat cgactacggc taggcacgga cctcgccgtt gccgagaacg atccatttct
     4981 ggctggtcag cccttcaaga ccgaccgggc cacgcgcgtg aatcttgtcg gtcgaaatgc
     5041 cgatttcggc accgagtccg tactcgaaac catcagcgaa acgcgtcgag gcgttgacca
     5101 tcaccgacgc cgaatcgact tcgcgcaaaa agcgcatcgc cgaagtatag ttttcggtga
     5161 cgatggcgtc cgtatggtgg gaaccgtagg cgttgatgtg ctcgatcgcc tcgccgagcc
     5221 cgtccaccac cttgaccgaa atgatcggcg ccagaaattc gctggcgtag tcttcctcgc
     5281 tggctggccg cgcctccctg acccgatcca gggtttccgg acagccgcga atctcgacgc
     5341 ccttccgggt cagcatggcc gcgatcaccg gcaacaggcg aggcaccacc gtgcgcgcga
     5401 gcaacagcga ttcggcggta ttgcaggtgc cataacgctg ggtcttggcg ttctcgacga
     5461 tctgcagcgc tttttcagga tcggcatcag catcgaggta aacatgacag ttgccgtcga
     5521 ggtgctggat catcggcacg cgcgcctcgg cgagcaggcg cgagatcagt cccttgccgc
     5581 cgcgcggaac gatcacgtcg acaaactcgc gcatcctgat caggtggccg accgcggcgc
     5641 ggtcggtggt ttcgatcacc tgcacggctg tcgccggcag cccggcgctg gccagtcctt
     5701 cctgtaccag tgcggcaatg gcctggttgc agcggatcgc ttccgagccg ccgcgcagaa
     5761 tcgacgcatt gcccgacttc aggcacaggg ccgcggcgtc ggcggtgacg ttcggacgcg
     5821 cctcgtagat gatcccgatg acgcccagcg gcacgcgcat cctgcccacc tgaatgccgc
     5881 tcggtcggaa cttcaggtcg ctgatctcgc ccaccggatc gggcagcgcc gcgacctgct
     5941 cgacgccctc ggccatcgcc gcaacgccct gctccgaaag cgtcaggcgg tcgagcagcg
     6001 ccggttccag accggccgca tgggccgccg ccagatcctc ccgattggcg gcgaccagcg
     6061 tgccacactc ccgccggata gccgcggcaa tggcccgcag cgcagcgttc ttcgcctggc
     6121 tgtcggcgcg ggcgatcagc cgcgaagctt cgcgggcctg cctgccgacg cgctgcatgt
     6181 actgctcgat gttcatgagg ttccctgttt taccgacaga gtgattatag ccaccgacgg
     6241 tgcgcaagcg tcaacggaac ccctggaaag gaatacactc tgaggcttat cgcaattttc
     6301 cgccgccgcc gaccatgaag aaaaaaatga ttctgcccgc tgaagtgaaa gtctgcgcaa
     6361 cctgcagcta ctgggacggt gaacgaacgg tcgatcccga actgcgtatg gtggtcgtcg
     6421 ccgaggattg cgcgggtgag tgcctggtgc agaccaaatg cagccatggc ctcaacgacg
     6481 tacgcgccga aaccgaacag tgcgcctggg aacacctggc acctgacgtc gcccccggtg
     6541 acgatggaaa gggcatcagc gcctgacccg gcgggcaagt gcttgcttca ggcggctgcc
     6601 aggcgaagac ccagttgccg gaactcgtcc catacatcgc cgctggccag ccccttgatc
     6661 atccggtcga tacgcgcggc gtgcgccagc gccagctcgg cccgcgccct gtcgagccgc
     6721 ttcaatgcct gcctgatcag cgtctgccgt gccccccaga tacgcgcgtc gcgcagcaac
     6781 gcatcgagcg gctgttgcct ggccagcccg gtgctgatct gggccagcgc ccgcacttcc
     6841 tcggccatcg cccagaggat cagcagtggc gcctcccctt cctgctgcag tccgtcgatg
     6901 gcgcgcacca gacgcgcgac atcgccgacg agcagcgctt cgcgcaggcc gtcgaggtcg
     6961 taacgggcga cgttgaggac ggcgtcgcga atctgctgcg gactcagctc accatcgggg
     7021 taaagcacgc ccagcttcag tatctcctgg tgtgccgcca ggagattgcc ttcgacgcgc
     7081 tcggcgatga agcgcaaacc ctcggcggac gcgctctgct tctggcggcg caggcgaccg
     7141 gcaagccagg ccggcagttc gcccaggccc ggcgtcggca acttgaccac cacgccggcg
     7201 gtggccaagg cactcatcca cgcggatttt tcctctcgcc agtcgacgcc gatcatgctc
     7261 accagcaaca gcgaatcggg tgaaggacgc gtgcaatact cctgcagcgc ggcgccgcct
     7321 tcgcgtcccg gtttgccggt cggaatacgc agatcgatga gcgtacgacc accgaagagc
     7381 gacaggctgc cggcggcgtg atacaggccg ttccagtcga aaccggcgat cgcnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagttcga tggtcgccgg cggcaccaga
     7561 tcgcgcccct tgtcgtcggt gagccggtag ttgtaaagat aacgaagacg cagctcactg
     7621 atccgaccgg agcccgagag cgaaaggatc acccggtcac gatactcggc gcccggcaac
     7681 aaggtggcct gcgcgtcgct ggccgtcaac gccagacgcg tcgaccccga ggcacgaatg
     7741 gcccgcttca ggctggcacc gatcaccgaa tattccggca tcgacagata caggctttcg
     7801 aaaggcaagg tgtaggcgcc gcgcaactga aagccgcaac cggcaagcag cgcgagcaca
     7861 acggcaagcc aggcagacgg tagcgctgca cgcatggtga tcgtcctcga agtcttgatg
     7921 ggctggcgat gcgcgtcagg caacgatatt caccagacgg ccgggaacga ccaccagttt
     7981 cctcggtgcg gcaccagcca gatgcttgcg cgccacttcg caggccagcg cagcactctc
     8041 gatggccgcc cgatcggcac cagccggcac gcgcaggctg ccgcgcagct tgccgttgat
     8101 ctgcagcacc agttcgattt catcctgcac cagcgcgccg gcgtcaacta ccgggaacgc
     8161 ctcctggctg gccgtgtgac cgggtctgag ggccgcatac agtgcctcgc agacatgcgg
     8221 gacgatcggg ctgagcatca gcgtcaccgc ttcgagcgct tcctgcctga ccgcgcgcgc
     8281 ggcaggcgag tcgtcggcga tccggccata ggcgttgagc agttccatca ccgccgcgat
     8341 cgcggtattg aactgcttgc ggcggccata atcgtcggcg accttgccga tcgtctgatg
     8401 caactgccgg cgcaaggctt tcagttcacc ggacaaatcg gctgccggag cggccgcgac
     8461 aggccccgcc gagacatgct cgtagacgat tcgccacagt cgcttgatga aacggaaggc
     8521 accttcgacg ccggcgtccg accattcgag cgactggtcg ggcgggctgg cgaacatgat
     8581 gaacaggcgc gccgtgtcgg ccccatactg gtcgatcagc gcctgcgggt cgaccccgtt
     8641 gttcttcgac ttcgacatct tctcgatgcc cccgacgacg accggcaaac catcggcgcg
     8701 cagggtcgcc ccgctggcac ggccacgttc gtcatggacg gcatcgacgt cggccgggtt
     8761 gagccactgc ttcttgccct
//
LOCUS       DAOSIT010000023         8770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7527_length_8770_cov_10.052783, whole genome shotgun sequence.
ACCESSION   DAOSIT010000023 DAOSIT010000000
VERSION     DAOSIT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_8770_cov_10.052783"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLL14_01245"
     CDS             <1..750
                     /locus_tag="PLL14_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG02570.1"
                     /translation="EEINRVLTDQIADRLYTTERSAQANLAREGIAEERVCFVGNVMI
                     DSLLSNREFAHSPTKTLQMAGVDPAVLDDRRGYGVVTLHRPSNVDRAEQLAPLLSVLR
                     EVSEQLPLVFALHPRTRANIDRFGLGEILASPRIVLLPPQGYLEMLGLLAGAKLVLSD
                     SGGLQEETTALGVPCLTIRENTERPITVEQGTNTLVGCDPQAIRKGVGEILSGTGKTG
                     RVPELWDGRAAERIAADLSQWLLARHARRGS"
     gene            766..1632
                     /locus_tag="PLL14_01250"
     CDS             766..1632
                     /locus_tag="PLL14_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOG02571.1"
                     /translation="MPGQPLTNALTIDVEDYFQVSAFAPYIARDRWDACECRVERNVE
                     RILLMLEEMHTRATFFTLGWIAERYPQLVRRIVDGGHELASHGYGHQRASDLIATAFF
                     ADVYTAKKLLEDISGHPVIGYRAPSFSIGEGNLWAFDCLERAGYRYSSSIYPIRHDHY
                     GMPDAPRFSHQVRPGLLELPVTTARFFNRNWPASGGGYFRLLPYALSRWLLQKVNTRD
                     QQAAIFYFHPWEIDAGQPRVAGINARTRFRHYVNLQHTEGRIRRLLADFRWGRVDEVF
                     LGGGCPAAGVGQ"
     gene            1629..2717
                     /locus_tag="PLL14_01255"
     CDS             1629..2717
                     /locus_tag="PLL14_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HOG02572.1"
                     /translation="MNSSREEVARLAAAATVPVSVRRLDPDDPGAVGRWDAFVEACQE
                     ATFFHKSGWQRIMREVFGHRTHFLYAERAGVVEGVLPLAQVKSLLFGNTLVSLPFAVY
                     GGVAASSPAAAEALEREAQVIARRLGVAHLELRNTQQRHPEWPLQDLYVRFRKVIAPE
                     VEANMLAIPRKQRAMVRKGIKNGLTSEIDRDPGRFFALYADNVHRHGTPALPRRYFDA
                     LQRVFGSDCEVLTVVDGAGRSLSTVMSFYFRDEVLPYYAGDELSARELAANDFKYWEL
                     MRRSCERGLKVFDYGRSKQGTGSYAFKKNWGFEPQPLFYEYCLYRRDAIPQNNPSNAK
                     YRLLIDTWRRMPIGLANWLGPFIVRNLG"
     gene            2801..>3627
                     /gene="xrtA"
                     /locus_tag="PLL14_01260"
     CDS             2801..>3627
                     /gene="xrtA"
                     /locus_tag="PLL14_01260"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HOG02573.1"
                     /translation="MNPAVPIPSSSATAAAWRVAWPALALTFLLILVLFRETAMAMAS
                     IWVRSDTYAHGLIVPPLTLWLIWRKRASLALLAPTHSYGALLLLAGAGFAWLLGELAA
                     VNALSQFALVFILIFIVPVVLGWRVARCIAFPLCFLLFAVPFGDFTQPKLMEWTAHFT
                     VLGLRLTGIPVYSEGQHLIIPTGSWSVVEACSGVRYLIASVTVGTLFAYLTYHSLARR
                     LTFVAISILVPIVANWGRAYMIVMLGHLSGNKLAVGVDHLIYGWVFFGIVIMAMFWIG
                     "
     assembly_gap    3628..3727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3728..4123
                     /locus_tag="PLL14_01265"
     CDS             <3728..4123
                     /locus_tag="PLL14_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOG02574.1"
                     /translation="NQDQQHKMVSSMNVLVTNDDPVWAKVAGGTREISFNQQALPVST
                     TQLRGPGGTRLVVWQWYWVNGQWTSSDALVKVYTALSRLTGKGDDSAVIVLYAPQGQA
                     GEGDAVLADFARAAGPAVSSALQQTLAIR"
     gene            4120..5307
                     /locus_tag="PLL14_01270"
     CDS             4120..5307
                     /locus_tag="PLL14_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOG02575.1"
                     /translation="MILAQDPRPLVAHVVFRFDVGGLENGVVNLINHMPADAYRHAVI
                     SLTEITDFRRRIVRDDVSFVALEKPPGHAIRIYPRLFSLFRQLRPAIVHSRNLAALEV
                     AMPAWAAGVPVRIHGEHGRDIGDLDGSSRKYQWLRRFYRPFVTRYIALSRDLEDYLTC
                     RVGVNPSRVSQIYNGVDAQRFHPAGTRQPIVGCPFSDSRCWLVGTVGRMQGVKDQTTL
                     VKAFIRALERFPVLRSDLRLVIIGDGPLRAQAQAMLEQAGLADRAWLPGQRDDVPEIL
                     RGLDCFVLPSLAEGVSNTILEAMASGLPVIATAVGGNGELIDQGSTGQLVAAADAEAM
                     AREIAGYALDPNRARAAGRAGRAVVEQQFSLEAMTQRYQGLYDQLLGSAAGRARRSTL
                     IHP"
     gene            5347..7281
                     /locus_tag="PLL14_01275"
     CDS             5347..7281
                     /locus_tag="PLL14_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase 1, exosortase A
                     system-associated"
                     /protein_id="HOG02576.1"
                     /translation="MCGITGIFDTRGQRDIDRAVLTRMNESQHHRGPDEGGLHVEPGL
                     GLGHRRLSIIDLSTGQQPLYNEDQSVCVVFNGEIYNYQELIPELLALGHVFHTRSDTE
                     VIVHAWEAWGEDCVRRFRGMFAFALWDRNRETLFLARDRLAVKPLYYALLPDGTFLFG
                     SELKSLLAHGGLAREIDPLAVEEYFALGYVAEPRTIFRQARKLPPAHTLTLRRGQPAA
                     APKEYWDLRFTLDAACSVDEACEQLDEKLRESIRLRMIAEVPLGAFLSGGVDSSAVVA
                     LMAGLSNDPVNTCSIAFDDPAFNEAQFAQKVADRYRTRHHCDLVASDDFDLVDTLASL
                     YDEPYADSSAIPTYRVCQLARRHVTVALSGDGGDETFGGYRRYRLHLMEERLRAAVPH
                     SVRRPLFGLLGRCYPKADWAPRMFRAKTTFEGMARTSVEAYFHSVSILRGPMRKQLFT
                     DRFRREIDGYSAEDVFRYHAGRADTDDPLALIQYLDLKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHELLEWMATLPSAFKVRGQEGKFLLKKAMEPRLPQDILYRPKMGFSVPLA
                     RWFRGPLRERVRDAVLGPRLAATGWFNEDYLRHVVDAHQSGVSDYSAALWTLLMFEAF
                     LRNVVDAPFDQAAVAGVAAQ"
     gene            7298..8497
                     /locus_tag="PLL14_01280"
     CDS             7298..8497
                     /locus_tag="PLL14_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOG02577.1"
                     /translation="MSNLLYLVHRLPYPPNKGDKVRSYHLLKHLAARHRVFLGTFIDD
                     PQDEAYVEAVREYCADLYLVRLKPYLARLLSLRGLLGNEPLTLPYYRHAGLQDWVDRT
                     LREQQIDAALIFSSVMASFVERAPRLRQLVDFVDVDSLKWGQYAANHRWPMSWLYRRE
                     GRRLLAFERAVAIRAERSFFVTQGEADLFRGLAPESAGKVEAMCNGVDADFFSPRNDL
                     SSPFRVDERAVVFTGAMDYWPNIDAVSWFAADILPRLRQNWPDLRFYIVGRSPTAAVQ
                     ALAGEHVVVSGTVPDVRPYLQHAAVVVAPLRLARGVQNKVLEAMAMGLPVVASRECST
                     AIDAVPERDFLTAGTVPEYVSQIEALLREPARSAAIGIAARQQVLARYSWDAHLSVID
                     RHLGGAT"
     gene            8652..>8770
                     /locus_tag="PLL14_01285"
     CDS             8652..>8770
                     /locus_tag="PLL14_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbuB"
                     /protein_id="HOG02578.1"
                     /translation="MRILHILDHSIPLHSGYTFRTLSILREQRKLGWQTFHLTT"
BASE COUNT         1407 a         2729 c         2812 g         1722 t
ORIGIN      
        1 gaggagatca accgcgtcct caccgaccag atcgccgacc gcctgtatac caccgagcgc
       61 agcgcacaag ccaacctggc gcgcgagggg atcgccgagg aacgcgtgtg ttttgtcggc
      121 aacgtgatga tcgattccct gctgtcgaat cgggagtttg cccattcccc gacgaagacg
      181 ctgcagatgg ccggtgtcga tccggcggtt ctcgacgatc gacggggtta tggtgtggtg
      241 acgctgcacc gtccgtcgaa tgtcgaccgg gccgaacaac tggcgccctt gctgagcgtg
      301 ttgcgcgaag tttccgagca actgccgctg gtctttgccc tgcatccgcg cacgcgcgcc
      361 aacatcgacc gcttcggcct tggcgagatc ctggccagtc cgcgcatcgt tctgctgccg
      421 ccgcagggtt atctggaaat gctcggcttg ctcgccggcg cgaaacttgt gctttccgat
      481 tccggaggcc tgcaggaaga aaccacggca cttggcgtac cctgcctgac cattcgcgag
      541 aataccgagc gtccgattac cgtcgagcaa ggcaccaata ccctcgtcgg ctgcgatccc
      601 caggcgatcc gcaagggcgt cggcgagatc ctgtccggta ccggcaagac cggacgcgtt
      661 cccgagctat gggatgggcg ggcggcagag cgcatcgccg ccgatctctc gcagtggttg
      721 ctcgcgcgcc acgcgcggcg cggcagctag aacgagtttc cgaaaatgcc cggccagccg
      781 ctgaccaacg ccctgacgat cgacgtcgaa gactacttcc aggtttcggc gttcgctccg
      841 tacatcgcgc gcgatcgatg ggacgcctgc gagtgccggg tcgagcggaa tgtcgagcgt
      901 attctgctga tgctcgaaga aatgcacacg cgtgcgacat tcttcacgct cggctggatc
      961 gccgaacgct atccgcaact ggtgcgtcgg attgtcgatg gggggcacga gctggccagt
     1021 cacggttacg gccatcagcg tgccagtgat ctgattgcca cggcgttttt cgccgacgtg
     1081 tatacggcca agaagctgct ggaagatatt tccggtcacc cggtcatcgg ttaccgggcg
     1141 ccaagcttct cgattggtga aggcaatctg tgggccttcg actgtctcga acgtgccggt
     1201 tatcgctaca gttcgagcat ctatcccatt cgccatgacc actacggcat gccggatgcg
     1261 ccacgctttt ctcaccaggt gcgtcccgga ttactcgaac tgccggtgac cacggcgcgt
     1321 ttcttcaacc gcaactggcc ggccagcggt ggtggctact ttcgcctgtt gccctatgcg
     1381 ctgtcgcgct ggctgctgca aaaggtcaat acccgcgatc agcaggcggc gatcttctac
     1441 ttccatccct gggagatcga cgccgggcaa ccgcgggttg ccgggatcaa tgccaggacg
     1501 cgtttccgcc actatgtcaa cctgcagcat accgaaggcc ggatcaggcg cttgctcgcc
     1561 gacttccgct gggggcgggt cgacgaagtg tttctcggcg gcggttgccc tgccgcaggg
     1621 gtcggtcaat gaacagcagc cgcgaggaag tggccaggct tgctgccgcg gcgaccgtgc
     1681 cggtcagcgt ccgccgcctt gatcccgacg acccgggggc cgtcggccgc tgggacgcgt
     1741 tcgttgaagc ctgccaggaa gccacctttt tccacaagtc cgggtggcag cgcatcatgc
     1801 gtgaggtctt cgggcatcgt acgcattttc tctatgccga gcgggcgggt gtcgtcgagg
     1861 gtgtcctgcc gctggcgcaa gtcaagagcc tgctcttcgg gaacacgctg gtgtcgcttc
     1921 cctttgccgt ttatggcggg gtggccgcca gcagcccggc ggcggccgag gctctggaaa
     1981 gggaggcgca agtcatcgcc aggcgcctcg gcgttgccca tctggagttg cggaacacgc
     2041 agcaacgtca ccccgaatgg ccgctgcagg atctctacgt ccgtttccgc aaggtgatcg
     2101 ccccggaagt cgaagccaac atgctggcca tcccgcgcaa gcagcgggcg atggtgcgca
     2161 agggcatcaa gaatggcttg accagcgaga tcgatcggga tccgggccgg ttcttcgcac
     2221 tctacgcgga caacgtccat cggcacggga caccggcatt gccgcggcgt tatttcgacg
     2281 ccctgcaacg ggtgttcggc agcgattgcg aggtgcttac ggtggtggac ggcgccgggc
     2341 gttccctgag cacggtcatg agtttctact ttcgtgacga ggtcctgccg tattacgccg
     2401 gcgacgagtt gtcggcgcgc gaactcgcgg ccaacgattt caagtactgg gagttgatgc
     2461 gccgctcctg cgagcgtggg ctgaaggttt tcgactacgg ccgcagcaag cagggcaccg
     2521 gctcctatgc cttcaagaag aactggggct tcgagccgca gccgctgttc tacgagtatt
     2581 gcctttatcg ccgtgatgcc attccgcaga acaacccgtc caatgccaag taccgtctgc
     2641 tgatcgacac ctggcggcga atgccgatcg gcctggccaa ctggcttggg ccgttcattg
     2701 tgcgaaacct cggttgagca gcccaccagc gcgtttccct tggcggtgat cacgcggaac
     2761 tgatgacttc cgaagccagc aatttgctga aagccactcc atgaacccag ccgtgccgat
     2821 tccgagttct tctgccaccg ctgccgcctg gcgagtcgcc tggcctgccc tggcgctgac
     2881 cttcctgctg atcctggtac tctttcgcga gacggcgatg gcgatggcga gtatctgggt
     2941 ccgctccgat acctacgcgc atggactcat cgtgccgccg ctgacgctgt ggctgatctg
     3001 gcggaagcgt gcaagcctgg cgttactggc acccacccac agctacgggg cgctgctgct
     3061 gttggccggc gcgggtttcg cctggctgct cggcgagttg gcggcggtca atgcgctgtc
     3121 gcagtttgcg ctggtcttca ttctgatttt catcgtgccg gtggtgctcg gctggcgggt
     3181 cgcgcgctgc attgcctttc ccctgtgttt cctgcttttc gcggtgccct ttggtgattt
     3241 cacccagccg aagttgatgg agtggacggc gcacttcacc gtgctcggtt tgcgcctgac
     3301 gggcatcccg gtgtatagcg aagggcagca cctgatcatc cccaccggga gctggtcggt
     3361 ggtcgaggcc tgcagcggcg ttcgctatct gatcgcttcg gtgacggtgg gaacgctgtt
     3421 cgcctacctg acctatcact ctctggcgcg gcgcctgact tttgtcgcca tctcgatcct
     3481 cgtaccgatc gtcgccaact gggggcgtgc gtacatgatc gtgatgctgg gtcacctgtc
     3541 gggcaacaag ctggccgtcg gcgtcgacca cctgatctac ggctgggtgt tcttcggtat
     3601 cgtgatcatg gccatgttct ggattggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnaac caggaccagc agcacaagat ggtcagttcg atgaatgttc tggtgaccaa
     3781 cgatgacccg gtctgggcca aggtcgccgg gggcacacgc gaaatctcct tcaaccagca
     3841 ggcgctcccg gtgagcacga cgcagttgcg cgggcccggc ggcacccgtc tggtggtctg
     3901 gcagtggtac tgggtgaatg ggcagtggac ctccagcgat gcgctggtca aggtctatac
     3961 ggcgctgtcg cggctgaccg gcaagggcga cgactcggcc gtgatcgttc tctacgcgcc
     4021 gcaaggtcag gccggcgagg gcgacgccgt gctcgccgac tttgcacgcg ccgccgggcc
     4081 ggcagtgagc agcgcactgc agcaaacgct ggccattcga tgattctggc gcaagacccg
     4141 cgccccctgg tcgcgcatgt ggtttttcgt ttcgacgtcg gcggtctgga gaatggcgtc
     4201 gtcaacctga tcaatcacat gccggccgac gcctatcgcc acgcggtgat ttccctgacc
     4261 gagattacgg atttccgacg ccgcatcgtg cgtgacgatg tcagcttcgt cgccctggag
     4321 aaaccacccg gacacgcgat ccggatttat ccccgcctgt tcagcctttt tcggcagttg
     4381 cggccggcga tcgtgcacag ccgcaatctg gcggcactgg aggtggcgat gccggcctgg
     4441 gcggcgggtg ttccggtgcg catccacggc gaacatggcc gcgatatcgg cgatctcgat
     4501 ggttccagcc gaaagtacca atggctgcgg cgtttttatc gcccattcgt gacgagatac
     4561 atagcgctgt cgcgcgatct cgaagattat ctcacctgcc gggtgggagt gaatccatcc
     4621 cgggtgtcac agatttacaa cggtgtcgat gcgcagcgct ttcatccggc ggggacgcgt
     4681 cagccgatcg tcggctgtcc gttctcggat tcgcgttgct ggctggtggg taccgtcggt
     4741 cgcatgcagg gggtcaagga tcagacgaca ctggtcaagg ccttcatcag ggcacttgag
     4801 cgattccctg tgctgcgcag tgacctgcgg ctggtgatca tcggcgatgg accgttgcgg
     4861 gcgcaggcgc aggcgatgct cgaacaagcc ggactggccg accgggcgtg gttgccgggg
     4921 caacgtgacg acgttcccga gatcctgcgt ggtctggact gtttcgtgct gccatcgctg
     4981 gccgaggggg tatcgaacac catcctcgaa gcgatggcca gcggcctgcc ggtgattgcg
     5041 accgccgtcg gtggcaatgg cgagttgatt gaccagggta gtacgggcca gctcgtggcg
     5101 gcggccgacg ccgaggcgat ggccagggag atcgccggct acgcgctgga cccgaaccgt
     5161 gcgcgcgctg ccgggcgtgc gggacgagcc gtggtcgagc agcagttcag cctggaggcg
     5221 atgacgcagc gctaccaggg actctacgat cagttgttgg gttcagcagc cggcagggca
     5281 aggcgcagca ccttgatcca cccgtaggcg aacgcggcaa ctcaggaagg gaagcgaagc
     5341 aagactatgt gcggcattac cggaatcttt gatactcgtg gccagcgcga catcgatcgc
     5401 gccgtgctga cacggatgaa cgagtcgcag catcatcgtg gcccggatga gggcgggctg
     5461 cacgtcgaac cgggcctggg tctcggccat cggcggctct cgatcatcga cctgtcgacc
     5521 ggccagcagc cgctctacaa cgaggaccag agcgtttgcg tggtgttcaa cggggaaatc
     5581 tacaactatc aggaactcat tccggagttg ctggcgctcg ggcatgtctt ccatacgcgc
     5641 agcgacaccg aggtcatcgt ccatgcctgg gaagcctggg gtgaggactg tgtgcgccgt
     5701 tttcgcggca tgttcgcttt tgcgctctgg gatcgcaacc gcgagacgct ttttctcgcg
     5761 cgcgaccggc tggcggtcaa gccgctctac tacgccctgt tgcccgatgg cacctttctg
     5821 ttcggctcgg aactcaagtc gctgctggca catggcggcc tcgcgcgcga gatcgacccc
     5881 ctggcggtcg aggagtactt tgcgcttggt tacgtcgccg agccgagaac catctttcgt
     5941 caggcgcgca agctgccccc ggcgcatacc ctgaccctgc ggcgtgggca gccggcggcg
     6001 gcgccgaagg aatactggga cctgcgcttt accctcgatg ccgcgtgttc ggtagacgag
     6061 gcctgtgagc aactcgacga gaaactgcgc gagtcgatcc gcctgcgcat gatcgccgag
     6121 gtgccgctgg gggccttcct ctcgggaggg gtagattcca gcgcggtcgt cgcgttgatg
     6181 gccggtctct cgaacgatcc ggtgaatacc tgctcgattg cctttgacga tcccgcgttc
     6241 aacgaagcgc agtttgccca gaaggttgcc gaccgttacc gcacccgcca tcactgtgac
     6301 ctggtcgcca gcgacgactt cgatctggtc gatacgctgg ccagcctgta cgacgagccc
     6361 tatgccgaca gttcggcgat tcccacctac cgcgtctgtc agctggcgcg ccggcacgtc
     6421 accgttgccc tgtcgggtga cggtggcgat gaaacctttg gcggctatcg tcgctaccga
     6481 ctgcacctga tggaagaacg cctgcgcgcg gccgtgccgc atagcgtacg tcggccgctg
     6541 tttggtctgc tcggtcgctg ctatccgaaa gcggactggg cgccgcgcat gtttcgcgcc
     6601 aaaacgacgt ttgaaggcat ggcgcgcacc tcggtcgaag cctatttcca ttccgtatcg
     6661 atcctgcgtg gtccgatgcg caaacaactt tttaccgatc gcttccggcg agaaatcgac
     6721 ggctacagcg ccgaggacgt ctttcgctac catgccgggc gggcggatac cgacgatccg
     6781 ctggccctga tccagtacct cgacctcaag acctacctgg tcggcgacat caacaccaag
     6841 gtcgaccgtg ccagcatggc gcactcgctg gaggtgcgcg aacccttgat ggaccacgaa
     6901 ctgctcgaat ggatggcgac cctgccgtcc gcgttcaagg tccgcggtca ggaaggcaag
     6961 ttcctgctca agaaagcgat ggagccgcgc ctgccccagg acatcctcta tcgaccgaag
     7021 atggggtttt cggtgcccct ggcgcgctgg ttccgaggcc cactgcgcga gcgcgtccgc
     7081 gacgcggtgc tcggtccgcg cctggcggcg accggctggt tcaacgagga ttacctgcgg
     7141 cacgtcgtcg atgcccacca gtcgggcgtc agcgactaca gcgccgcgct gtggaccctg
     7201 ctgatgttcg aggccttcct gcgcaacgtc gtcgatgcgc cgttcgatca ggcggcggtc
     7261 gccggcgttg cggcgcaatg accctgggtg gccgcagatg agcaatcttc tctacctggt
     7321 gcatcgcctt ccctacccac cgaacaaggg cgacaaggtt cgctcgtatc acctgctcaa
     7381 gcacctggcg gccaggcacc gggtattcct cggcacgttc atcgatgatc cgcaggacga
     7441 ggcctatgtc gaggcggtgc gcgagtactg tgccgacctg tatctggtcc ggctcaagcc
     7501 gtatctggcg cgcctgctga gtctccgggg cctgctcggc aatgaaccgc tgacgctgcc
     7561 ctactatcgc catgccgggc tgcaggactg ggttgatcgc acgctgcgcg aacagcagat
     7621 cgacgccgcc ctgatttttt cctcggtcat ggcgagcttc gtcgagcgcg cgccgcggct
     7681 acgtcagttg gtcgactttg tcgacgtcga ttccctgaaa tggggccagt atgccgccaa
     7741 ccaccgctgg ccgatgtcct ggttgtaccg gcgcgagggc aggcgcctgc tggctttcga
     7801 acgtgcggtc gcgatccggg ccgaacgatc gttctttgtc acccagggcg aggccgacct
     7861 gtttcggggt ctggcgccgg agtccgccgg taaggtcgag gcgatgtgca acggggtgga
     7921 cgccgacttc ttctcgccgc gaaacgatct gtcttcacct ttccgcgtcg acgaaagggc
     7981 ggtggtgttt accggcgcca tggactactg gccgaacatc gatgcggtga gttggttcgc
     8041 cgccgacatc ctgcccaggc tgcgtcagaa ctggccggat ctgcgcttct acattgtcgg
     8101 acgcagcccg accgcggcgg tgcaggcgct ggccggagag cacgtggtgg tcagcggtac
     8161 cgtcccggac gttcgtccct acctgcagca tgcggcggtc gtcgttgcgc ccctgcggct
     8221 ggcgcgtggc gtccagaaca aggtcctgga ggcgatggcc atgggcctgc cggtcgtcgc
     8281 ctcccgggag tgttcgacgg ccatcgacgc tgtccccgag cgcgatttcc tgaccgcagg
     8341 aaccgtgccg gagtacgtca gccagatcga agccctgttg cgcgagccgg cacgttctgc
     8401 cgccatcggc attgccgcac gccagcaggt tctggcgcgc tacagttggg acgcccatct
     8461 ctcggtgatc gaccgccacc ttggcggggc aacgtgaaat actgccggtg cccggcaggc
     8521 gctctgtgta tgatcggctt ctccagatgc aggggcagca gcatgacgtt tagtcggtgt
     8581 tcggcgatgg tcttcaagac ctgcctgggt ggcgcacccg cgggcaggcg cagggagggt
     8641 ggccggtgag cctgcgcatt ctgcacattc tcgatcactc cattccactg cacagcgggt
     8701 ataccttccg caccctgtcg atcctgcgcg agcagcgcaa gctgggctgg cagacgttcc
     8761 acctcacgac
//
LOCUS       DAOSIT010000024         8673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7616_length_8673_cov_10.489209, whole genome shotgun sequence.
ACCESSION   DAOSIT010000024 DAOSIT010000000
VERSION     DAOSIT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_8673_cov_10.489209"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1695
                     /locus_tag="PLL14_01290"
     CDS             4..1695
                     /locus_tag="PLL14_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOG02579.1"
                     /translation="MMALAVGSLHAEDAQPGKKKALEEGPMQIGGSSPVGNIEMVHTL
                     NPKSPPMTKADFEVGKKIYFERCAGCHGVLRKGATGKALTTDITLERGSDYLRTFIFY
                     GSPQGMPNWGTSGTLSEKEVDIMTRYIQHEPPTPPEYGLQEMNATWKLIVPPSQRPTK
                     KMNNLDLANLFSVTLRDAGKIALIDGSSKKIIDVIDTGYAVHISRISKSGRYLYVIGR
                     DAKIVLIDLWMEKPAPVAEIRVGLEARSVETSKFKGYEDRYAIAGSYWPPQYTLMDGD
                     TLKPLKVIGTRGMTSDTNEYHPEPRVAAIVASHYHPEFLVNAKETGMVHMVDYSDLEN
                     LKVRMIKTPRFLHDGGFESTGRYFMDAANASDKIAVIDTKEGKLAAVVAVGKTPHPGR
                     GANFVHPKFGPVWATSHLGDDSVSLIGTDPVKHSQSAWKVVQTLKGSGGGSLFLKTHP
                     KSRNLWVDAPLNPDPAISQSVAVFDINNLDKGFTLLPIGEWAGVKPGSKRVVQPEYNR
                     DGDEVWFSVWSAGSEQSAIVVVDDKTRKLKAVIKDPRVITPTGKFNVYNTQNDIY"
     gene            complement(1818..2717)
                     /locus_tag="PLL14_01295"
     CDS             complement(1818..2717)
                     /locus_tag="PLL14_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG02580.1"
                     /translation="MPWKDWSAERLGIALAVLAALGFSFKAIFVKLAYAAAPVDAVTL
                     LSLRMVFALPAFLWVGFVASRSAPPLTRHDWSMLVALGLLGYYGASILDFIGLQYISA
                     GLERLILFTYPTLTVLIGVLFMGKSLTRREIGSLLLSYAGIGLAFAHDLQVAGEMTAV
                     LVGVAFVFGSSLSYACYLSGSAPAIQRLGAARFTALAMLVSTLATQLHFVATQPLTAL
                     IQPLPIYAYGAAMALFSTVLPVFMQSAAIRRIGSAKAVLIGTLGPILTIFFGWWLLAE
                     PLSAAQLGGAALVLAGVLLVSRR"
     gene            2815..3705
                     /locus_tag="PLL14_01300"
     CDS             2815..3705
                     /locus_tag="PLL14_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG02581.1"
                     /translation="MNYDLTDLRLFVAIAESQNLSRGAERVHLAPSSASHRMRLLEAG
                     IGTPLLLRQARGVSLTRAGEALLRHARQVFAQLEQMHADLAPYAKGVRGHVTLWANTH
                     ATHSFLPDDLAEFLRRYPQVSVTLAEHTSPEIVMAVARGEVEVGVVAERVEGAEVEML
                     PYRADRLVLITPGTHPLATQTSARLAEVLDYPFVMLHAGSAIHTFTMNAAANLGRHVN
                     VRIQVRSFEAVCRMVGAGVGIGLVPRSAVPQRGLREPPTVVTLDETWAQRDLQVCVRR
                     REDLSGFAADLVRSLAGCTG"
     gene            3924..4244
                     /locus_tag="PLL14_01305"
     CDS             3924..4244
                     /locus_tag="PLL14_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HOG02582.1"
                     /translation="MSEVTELSTANKQKLVSDMKVVVADAEEILRATAGVANDKMGDL
                     RERFGERLRDAKLRLADAEAALVDRTKAAARATDDYVHENPWRAVGVAAGIGLLLGVI
                     IGRR"
     gene            4261..4656
                     /locus_tag="PLL14_01310"
     CDS             4261..4656
                     /locus_tag="PLL14_01310"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOG02583.1"
                     /translation="MSETTGSGGGGLLAAARNSAATLLASGRTRLELLGNEIKAEKLR
                     AVHLLLVSQTVAFCLLVGILLAIALLLVLFWDNRVALLGSFSCLFFVIAGLAYLALKR
                     LTEAPENLFSTSIAELEEDLRRLHDTARK"
     gene            4653..4976
                     /locus_tag="PLL14_01315"
     CDS             4653..4976
                     /locus_tag="PLL14_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HOG02584.1"
                     /translation="MNQELIDLAIERGRLIERISSQRQLLGQQLQPMADALHATDRVA
                     ATVRSGCLYVRQHPHVVTAAVAVLVVLHPRRVWRWTTRGLFVWRTWRMLRREMADLGL
                     VPDRP"
     gene            complement(5052..5786)
                     /locus_tag="PLL14_01320"
     CDS             complement(5052..5786)
                     /locus_tag="PLL14_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG02585.1"
                     /translation="MERDSLIATSLIDAARQLSANLVPLHFSAPVSHIYNPLTYAWGA
                     HELYLRRYGAGPRRVVFLGMNPGPFGMVQCGVPFGEVTVVRDWLGITSGVQKPELENP
                     ARPIEGFACRRSEVSGKRLWGLFRERFGSPDVFFREHFVANYCPLAFFDHARNLTPDK
                     LVAAEAVPLRAVCDAHLQALVGALQPEWVIGVGAFAAARAAEVLAGSSIRTGRVLHPS
                     PASPLANRGCWAVAATEQLQALGVWR"
     gene            complement(5753..>5887)
                     /locus_tag="PLL14_01325"
     CDS             complement(5753..>5887)
                     /locus_tag="PLL14_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HOG02586.1"
                     /translation="TAGALVESMGWISFFLLCFVLAAPGMLLLLKVAPWNEIRSSPQV
                     "
     assembly_gap    5888..5987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5988..6784)
                     /locus_tag="PLL14_01330"
     CDS             complement(<5988..6784)
                     /locus_tag="PLL14_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG02587.1"
                     /translation="MPGWLRLLLTRQMLICVFTGFSSGLPLYLLLNLLPAWLRSEGVN
                     LKTIGFFALIQFPYTWKFLWSPLLDRYRLPLGRRRGWMLLSQLGLLFCIGLLGGFSPG
                     SDLVPVIWLSVVLAFLSATQDVALDAFRREILSDQELGLGNSVHVNAYRIAGLVPGSL
                     SLILADLLPWSQVFWITAAFMLPGMVMVLVVREPAVLGVPKTLRQAVVEPFHEFIGRQ
                     GWRGAMLVLGFIFLYKLGDSLATALATPFYLDLGYSKTDIGVIAKHAG"
     gene            complement(6784..7407)
                     /gene="metW"
                     /locus_tag="PLL14_01335"
     CDS             complement(6784..7407)
                     /gene="metW"
                     /locus_tag="PLL14_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOG02588.1"
                     /translation="MTEHDRRVRAEQRERFDFAVIANWIPPGERVLDLGCGDGRLLRY
                     LSETRDVTGYGVEIDRESVLGCIRNGVDVIQMDIECGLSGFEDASFEHVIISQALQTM
                     HTTERILGEMLRVAAEAVVSFPNFAYRTNRAAIAAGHMPVSEDLPYDWFDTPNVRFFT
                     IVDFENLCARLGIEIRERLAFDEAGRAVSEDPNLNGSLAFYRLGRRT"
     gene            complement(7404..8531)
                     /locus_tag="PLL14_01340"
     CDS             complement(7404..8531)
                     /locus_tag="PLL14_01340"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOG02589.1"
                     /translation="MSQENSVGFVTPQRAYFNEPLKLKSGAELPRFELVFETYGTLNS
                     ERSNAVLVCHALSGSHHVAGCYADDPENVGWWNNLVGPGKALDTRKFFVVGVNNLGGC
                     YGSTGPLSLNPETGKRYGAAFPLVTVEDWVAAQARLADHLGVQTWAAVIGGSLGGMQA
                     LEWSLQFPDRIRHAMVIASAPKLSAQNIAFNEVARQAIISDPDFHGGDYAEHGVVPTR
                     GLRLARMIGHITYLSDTQMAEKFGRQLRHGEHKFSYEVDFEIESYLRYQGDKFAAFFD
                     ANTYLMTTKALDYFDPAYDFDGNLAAALARAKASFFVAGFSTDWRFAPARSREIVFAL
                     LHNRLHVVYAEIDCSAGHDSFLLDDAHYHALLRAYLENIAT"
BASE COUNT         1842 a         2740 c         2597 g         1394 t
ORIGIN      
        1 gccatgatgg cgctggcggt cggtagcctg catgcggagg atgcacaacc cggcaagaag
       61 aaagcgctcg aagaggggcc aatgcagatt ggtggttcgt cgccagtcgg gaacatcgaa
      121 atggtacata cgctgaaccc gaaatcgcca cccatgacca aggccgattt cgaggtcggc
      181 aagaagatct acttcgagcg ttgcgcgggg tgccatggcg tattgcgcaa gggcgcgacg
      241 ggcaaggcgc tgacaacgga catcaccctg gagcgcggct ccgactatct gcggaccttc
      301 atcttctatg gttcaccgca aggcatgcca aactggggga cttccggcac gctcagcgag
      361 aaggaagtgg acatcatgac gcgctacatc cagcacgaac cgccgacccc gccggagtac
      421 ggtctgcagg aaatgaacgc gacctggaag ctgatcgttc cgccgtcgca gcgcccgacg
      481 aagaaaatga acaacctcga cctggccaat ttgttctcgg tgacgctccg cgacgccgga
      541 aagattgcgc tgatcgacgg cagcagcaag aagatcatcg acgtcatcga taccggctac
      601 gccgtccaca tttcgcgcat atcgaagtcc ggtcgttacc tttatgtgat tggccgtgac
      661 gccaagatcg tcctgattga tctgtggatg gagaaaccgg caccagttgc ggaaatcagg
      721 gtcggcctgg aagcgcgctc ggtcgaaacc tcgaagttca agggctacga ggaccgctac
      781 gcgattgccg gcagttactg gccaccacaa tacacgctga tggacggcga tacgctcaag
      841 ccgctcaagg tgatcggcac acgcggcatg acctcggaca ccaacgagta ccaccccgag
      901 ccgcgggtag cggcgatcgt cgcgtcgcac tatcaccccg agttcctggt taacgccaag
      961 gaaaccggca tggtgcatat ggttgactac agcgatctcg aaaacctcaa ggtcaggatg
     1021 atcaagacgc ctcgcttcct gcacgatggc ggcttcgagt ccaccggtcg ctacttcatg
     1081 gatgcggcca acgcctcgga caagattgcc gtgatcgaca ccaaggaggg caaactggcc
     1141 gcggtggtgg ccgtcggcaa gacgccgcac cccgggcgcg gtgcaaactt cgtgcatccg
     1201 aagttcggac cggtctgggc gaccagccac ctcggtgacg acagcgtgtc gctgatcggt
     1261 accgatccgg tgaaacacag ccagtccgcg tggaaagtgg tgcaaaccct caagggctct
     1321 ggtggcggat cgctgttcct gaagacccat ccgaagtcga gaaacctgtg ggtcgacgca
     1381 ccgctgaatc ctgatccggc aatttcgcag tcagtcgcgg tgttcgacat caacaatctg
     1441 gacaaaggct tcactctcct gccgatcgga gaatgggccg gtgtcaagcc gggttccaaa
     1501 cgcgtcgtgc aacccgagta caaccgcgat ggcgatgagg tctggttctc cgtctggagt
     1561 gcaggcagcg agcagtcggc gatcgtagtt gtcgacgaca agacgcgcaa gctcaaggcg
     1621 gtcatcaagg atccacgcgt gattacaccg accggaaaat tcaacgtcta caacacccag
     1681 aacgacatct actgagggat gccgggcacc gaccggcggg ggacctcgtg accccgtccg
     1741 gtcgactacg gctttgcgca gagtgtgcga tccggcgtcc gtcaagagca ccgcgccacc
     1801 cgacgcacca ccgcaatcta cctccggctg accagcagca ctccggccaa gaccagagcg
     1861 gcaccgccca gttgcgccgc cgacagcggc tcggcaagca accaccagcc gaagaagatg
     1921 gtcagtatcg gacccagcgt gccgatcagc acggccttcg ccgatccgat gcgacgaatc
     1981 gccgccgact gcatgaatac cggcaggacc gtcgaaaaga gggccatcgc agcgccatag
     2041 gcgtatatcg gcaacggctg gatcagcgcc gtgagcggct gcgtcgcaac aaaatgcaac
     2101 tgcgtggcca gcgtcgacac cagcatcgcc agcgccgtga agcgggctgc gccgaggcgc
     2161 tgaatggccg gcgcgctgcc ggaaagatag caagcgtagg acagcgaaga cccgaaaacg
     2221 aaagccacgc cgaccagcac cgccgtcatc tcgcccgcca cctgcagatc gtgcgcaaag
     2281 gccaggccga taccggcgta ggaaagcaac agggaaccga tctcgcgccg ggtcagcgac
     2341 ttgcccatga acaacacacc gatcagcacg gtcagcgtgg ggtaggtgaa caggatcaga
     2401 cgctcaaggc cggccgaaat gtactgcaag ccgatgaagt cgaggatgct cgcaccgtag
     2461 tagccgagca gacccagtgc caccagcata ctccagtcgt ggcgggtcag cggcggcgcc
     2521 gagcggcttg cgacgaagcc gacccagaga aaggccggca gcgcaaacac catgcgcagc
     2581 gacaacaacg tgaccgcatc gaccggtgca gccgcgtatg ccagcttgac gaagatcgcc
     2641 ttgaacgaga aaccgagcgc tgcaagcacg gcgagggcga tgccgaggcg ttcggcggac
     2701 cagtctttcc agggcatgga tcagccagaa tgagtgaaaa ccgaatgatg ccgctcacca
     2761 aatcgccaaa caattgctat attgacaagc ctgcgttcgt cagaaacgaa tggtatgaat
     2821 tacgacctca ccgacctgcg tctcttcgtg gccatcgccg agtcgcagaa cctgagccgc
     2881 ggcgcagaac gggtgcacct cgcgccttcc tcggccagcc atcgcatgcg cctgcttgaa
     2941 gcgggtatcg gtacgccgtt gctgcttcga caggcacgtg gcgtttccct gacccgcgcc
     3001 ggcgaggcgc tgctgcggca tgcgcgacag gtctttgccc aactcgaaca aatgcacgcc
     3061 gacctcgcac catacgcgaa aggcgtgcgt gggcacgtga ccttgtgggc caatacgcac
     3121 gccacgcaca gcttcctgcc tgacgacctg gctgaattcc tgcgccggta cccgcaggtc
     3181 agcgtcacgc tggccgagca caccagtccc gagatcgtca tggccgtggc acgcggcgag
     3241 gtcgaggtcg gcgtggttgc cgaacgcgtc gaaggggccg aggtggaaat gctcccttac
     3301 cgcgccgacc gcctggtgct gatcaccccc ggcacgcacc cgctggcgac acagaccagc
     3361 gcccgtctgg ccgaggtgct tgactacccc ttcgtgatgc tgcacgcggg ctcggccatc
     3421 cacaccttca cgatgaatgc cgctgccaac ctcggccgtc acgtcaacgt gcgcatccag
     3481 gtcaggagct tcgaggccgt ctgccgcatg gtcggagccg gcgtcggcat cggcctggtc
     3541 ccgcgcagcg ccgttccaca gagggggttg cgcgaaccac ccaccgtggt aaccctggac
     3601 gagacctggg cacagcgcga cctgcaggtg tgcgtgcgca ggcgcgagga cctgtcggga
     3661 ttcgctgcag atctggtgcg cagccttgca ggctgcaccg gctaacggct ggaaaaccgc
     3721 gccctgaccg cagcagcgag gccaacggcc ccaacagggt caaatccagc ctccggccac
     3781 cgcacccgcc atgaacgcgg ccgcttgtgg cggttggcat tgctctcggc gcgcgatttg
     3841 tataacctga taacctagtt gcaggcgtta cggctgcgac atccaggctc ttcctgaaat
     3901 caccttccac tgcgaggttc atcatgtccg aagtaaccga actttccacc gccaacaagc
     3961 aaaaactcgt ctccgacatg aaggtcgtcg tcgcagacgc agaggaaatc ctgcgcgcca
     4021 ccgccggcgt ggccaacgac aagatgggtg atctgcgcga gcgcttcggt gagcggctgc
     4081 gcgacgccaa actgcgtctg gccgatgccg aagccgcact ggtcgaccgt accaaagccg
     4141 cagcccgcgc caccgacgac tatgtccacg agaatccatg gcgcgcggtc ggcgttgccg
     4201 ccggcatcgg tctgctgctc ggcgtgatca tcggccgccg ctgaacgaca cctccaggac
     4261 atgagcgaaa ctaccggcag tggtggcggc ggcctgttgg cagccgccag aaacagcgcc
     4321 gccaccctgc tggccagcgg gcgaactcgc cttgaactcc tcggcaacga gatcaaggcg
     4381 gagaagctgc gcgcggtcca cctgctgctc gtgtcgcaga ccgtggcatt ctgtcttctg
     4441 gtcggcatac tcctggccat cgccttgctg ctggtcctgt tctgggacaa cagggtggcc
     4501 cttctgggat cattttcctg cctcttcttt gttatcgccg gcctcgcgta cctggccctg
     4561 aaacggttga ccgaagcgcc tgaaaacctt ttcagcacca gcattgccga gttggaagaa
     4621 gaccttcgtc ggttgcacga cacggcccgc aagtgaatca agagctgatt gatctggcca
     4681 tcgagcgcgg caggctgatc gaacggatca gcagccagcg gcaactgctt ggccagcaac
     4741 tgcaacccat ggccgatgcc ctgcacgcca ccgaccgagt cgccgccacg gtccgcagtg
     4801 gatgcctcta cgtccgccaa cacccgcatg tggtcactgc cgccgtcgcc gtgctggtcg
     4861 tgctgcatcc tcggcgcgtc tggcgctgga caacacgcgg tcttttcgtg tggcgcacct
     4921 ggcgcatgct gcgcagggaa atggcagacc tcggtctggt gcccgaccgc ccataacgga
     4981 cagacgcagg tccaagcaca tcgccccgat gcgatcatcc ggtgcgatac ctgcacaaca
     5041 cggcaaggaa ctcaccgcca gacgccgagc gcctgtagtt gctccgtcgc cgccaccgcc
     5101 cagcagcccc gattggccag cgggcttgcc gggctgggat gcagcactcg cccggtcctg
     5161 atgctgctcc cggccagcac ctcggctgcc cgtgctgccg caaaggcacc gacgccaatc
     5221 acccactcgg gctgcaaggc gccgaccagc gcttgcaggt gagcgtcgca aaccgcgcgc
     5281 aatggtaccg cctccgctgc gacaagtttg tctggggtca gattgcgtgc gtggtcgaag
     5341 aaggccagcg ggcaatagtt ggccacgaaa tgttccctga aaaacacgtc gggggaacca
     5401 aagcgctccc gaaacaagcc ccacaggcgt tttccgctga cttccgaacg cctgcaggca
     5461 aagccttcaa ttggtctggc gggattctcg agttccggtt tttgcacgcc ggaggtgatt
     5521 cccaaccagt cgcgcaccac cgtcacttca ccgaaaggaa cgccgcattg caccatcccg
     5581 aaaggccccg gattcatgcc cagaaagacc actcgcctgg gaccagcgcc ataacggcgc
     5641 aagtacaact cgtgcgcacc ccaggcatag gtcagcgggt tgtagatatg gctgaccgga
     5701 gccgagaaat gcagtggcac caggtttgcc gacaactgcc gggccgcgtc gatcagactt
     5761 gtggcgatga gcgaatctcg ttccatggcg caaccttgag cagcagcagc atgcccggcg
     5821 cggcgagcac aaagcacaac aggaagaaac tgatccatcc catcgactcc accagcgcgc
     5881 ccgccgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg gcatgtttgg
     6001 caatcacccc gatatcggtc ttgctgtagc cgagatccag ataaaagggg gtcgccaggg
     6061 ctgtcgccag cgaatcgcca agcttgtaga gaaagatgaa accgagcacg agcatggcgc
     6121 cgcgccagcc ctggcggccg ataaactcgt gaaagggctc gacgaccgct tgccgcaggg
     6181 tctttggcac ccccagcacg gccggttcgc ggacgaccag caccatcacc atgcccggca
     6241 gcatgaaagc tgcggtaatc cagaacacct gcgaccaggg aagcaggtcg gcaagaatca
     6301 gcgacaagga tcccggcacc agaccggcaa tccgataggc gttgacgtgt accgagttac
     6361 cgaggccgag ttcctgatcg ctcaggatct cgcggcgaaa cgcatccagg gcaacatcct
     6421 gggttgccga cagaaaggcc aggactaccg acagccagat caccggtacc agatcgctgc
     6481 ccggcgagaa gcctccgagc agaccgatgc agaacaacag ccccagttgc gagagcagca
     6541 tccagccacg gcggcgcccc agtggcagcc gatagcggtc gagcaacggc gaccagagga
     6601 acttccaggt gtacggaaac tggatcaatg cgaagaagcc gatggtcttg agattgaccc
     6661 cctcgctgcg cagccatgcg ggcaacagat tgagcaacag gtacagcggc aggcccgaac
     6721 tgaaaccggt gaatacgcag atcagcatct gccgggtcag cagcaggcgc agccagcccg
     6781 gcatcaggtt cttcgtccga ggcgataaaa ggcaagactg ccgttgagat tgggatcctc
     6841 ggaaaccgca cgcccggcct cgtcgaaggc cagtcgctcg cgtatttcga taccgagtcg
     6901 agcacaaaga ttctcgaagt cgacgatcgt gaagaagcgc acattcggcg tatcgaacca
     6961 gtcatacggc aggtcctcgg ataccggcat gtgcccagcg gcgatcgccg cccgattggt
     7021 gcggtaggca aaattgggaa agctgacgac cgcctcggcc gcgacgcgca gcatttcgcc
     7081 gaggatgcgc tcagtggtgt gcatcgtctg cagcgcctgc gagatgatca cgtgttcgaa
     7141 ggaagcgtcc tcaaatcccg aaaggccgca ctcgatatcc atctgaatca cgtcaacgcc
     7201 gttgcggatg caccccagaa cgctttcgcg gtcgatttcg acaccgtagc ccgtgacgtc
     7261 acgggtttcg ctcaggtagc gcaacaacct gccgtcgccg cagccgagat cgaggacacg
     7321 ttcgccaggc ggaatccagt tggcgatcac cgcaaaatcg aagcgttcgc gctgctcggc
     7381 cctgactctc ctatcgtgct cggtcatgtg gcgatgttct ccaggtaggc gcgcagcagg
     7441 gcatggtagt gtgcgtcgtc cagcaaaaac gagtcgtgcc cggcactgca gtcgatctcg
     7501 gcgtaaacga catgcaagcg gttgtgcagc agggcgaaga caatctcgcg cgaacgcgcc
     7561 ggcgcgaaac gccagtcggt tgaaaaaccg gcaacgaaaa agctggcctt ggcacgcgcc
     7621 agagccgccg ccagattgcc gtcgaagtcg taggcgggat cgaaatagtc gagcgccttg
     7681 gtggtcatca gataggtatt ggcatcgaaa aaagcggcga acttgtcgcc ctgatagcgc
     7741 aggtaggact cgatctcgaa atccacctcg taactgaact tgtgttcgcc atgccgtaac
     7801 tgccgaccaa atttctcggc catctgcgtg tccgacaggt aggtgatgtg gccgatcatg
     7861 cgcgccagtc gcaaacccct ggtcggaacg acgccgtgct ccgcgtagtc accgccgtgg
     7921 aaatcagggt cggagatgat cgcctgcctg gccacttcgt tgaaggcaat attctgggcc
     7981 gacagcttcg gcgccgaggc aatgaccatg gcgtggcgga tgcgatccgg gaattgcagc
     8041 gaccattcga gcgcctgcat gccgccgagg ctgccgccga tgaccgcggc ccaggtctgc
     8101 acgcccagat gatcggccag ccgggcctgt gcggccaccc agtcctcgac cgtcaccagc
     8161 ggaaaggcgg caccgtaacg tttgccggtt tccggattga gcgatagcgg ccccgtcgat
     8221 ccatagcacc cgcccaggtt attgacgccg accacgaaga acttccgggt gtcgagcgcc
     8281 tttcccgggc cgacgaggtt attccaccag ccgacgttct ccgggtcgtc agcataacag
     8341 ccagccacgt ggtggctacc ggaaagcgca tgacaaacca gcacggcgtt ggaacgttcg
     8401 ctgttgagcg tgccgtaggt ttcgaatacc agttcgaagc gaggcaactc ggcaccgctc
     8461 ttcaacttca gcggttcgtt gaagtacgct cgctgcggcg tgacgaatcc gacagaattc
     8521 tcttgtgaca tcgtgatcgt atcggtacca agggcagaaa aaacccggtc ggctgccaaa
     8581 gcggaccggg cttgccgcgc tttagccgta tttgtagggc gcccgcaagc tgatcgttca
     8641 aatcggcgcc aaggcggcga aagttacccg cag
//
LOCUS       DAOSIT010000025         8440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7853_length_8440_cov_7.587716, whole genome shotgun sequence.
ACCESSION   DAOSIT010000025 DAOSIT010000000
VERSION     DAOSIT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_8440_cov_7.587716"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..880
                     /gene="radC"
                     /locus_tag="PLL14_01345"
     CDS             149..880
                     /gene="radC"
                     /locus_tag="PLL14_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG02590.1"
                     /translation="MKAQADSRAKAQPGERPETLHYHLHRQRLRDRFEKAGFAGLAEH
                     EVVELLLTLAIPRVDVKEQAKALLRRFGNLKGIMDAPIGELRGAAKIGAVAPVAIKII
                     KELATLYLQQDAEGSDCLAEPAKLENLWRMRIGGLPNEVFEVTYLDSAYRPLRDGVER
                     LEEGTIDRAAVYPRKVIEAALKRGAAAIVLAHNHPNGAVAPTEQDKLLTRAIVLAAET
                     VRVRVLDHLIVSTNEAFSFKKAGLL"
     gene            877..3732
                     /locus_tag="PLL14_01350"
     CDS             877..3732
                     /locus_tag="PLL14_01350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOG02591.1"
                     /translation="MSIAPAIRKHYPHLNDAQRAIVDHEDGPLLVIAGPGSGKTHCIV
                     LRALNLLVQEKATPKQLVMCTFTEKAAFEMRDRLASAARKVGYAGDLSELTVSTIHSF
                     CNRILSQHRHRTPLGHGFETLDDLSQLLFIFEQFDQIIGPQTEGFYLRRWSTRWTAIE
                     GARNYFGKITEELVDPVQLVAAADPFLTEIGNAYLRYEHALQEANRIDFAHLQRAVID
                     LLADPETAEAVTRDIRHVLVDEYQDTNHIQEQLLLKLTEKSRNLCVVGDEDQSLYRFR
                     GATVRNILEFPQRMPGCAVVKLTTNYRSHRDIVEKYNRWMASADWRNPNGPAFRYDKT
                     IEADATQKFPDYPATFCIWGKNQRDEVARFADMAAFLKKNGVIQDFNQIALLLHSVRE
                     EHSGPYIAALEAKGIPAFCPRARSFFLIPEIRDLVACFALLFGWHGDGRGEVHGSIAE
                     LAAYVDDALVQLARRFGGQHPLAKTLQRWVSEIAALQGKDALDFRPADYFYHLLALDP
                     FKRAIHNENIARNLAIFSQLLNVFQGYYHYTVITAKNREYIRFHLFNSFLRLLFEGGI
                     NEYEDADQPFPKGHVQIMTIHQAKGLEFPVVVVGSLSKQLSSPKKIDRDLGAFYHRAP
                     FEPEGRVTAFDRMRLHYVAFSRPQKVLVLTAGEAPKDYFAPIWQGLPQWPYVQTELLA
                     AQRFELRERIPLKKTYSFTGDLRIYETCPRQYQFFREYDFTPSRSSVIFFGLLVHQTI
                     EDIHRIVLDGKLDTLNDKRIREIFDRTFRFMCLSDVRPIGETAKEGAFRQVMDYYRQN
                     REEMRRVIQTEVDVSLEKDGYILIGKVDLLLGGDGKLELLDFKSSARPRNSPDLIAAY
                     ERQLCTYAHILERRHNRQVDRLLLYWTAEPRKEDALMVLPYDRARVEEAGRCFDKTVG
                     RIQAKDFAVSTPPERTICKECDLRMLCCGEGIIEGFK"
     gene            3744..5561
                     /locus_tag="PLL14_01355"
     CDS             3744..5561
                     /locus_tag="PLL14_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOG02592.1"
                     /translation="MATIDIIKTELVWPGKYNDDGTLKEVPRVNLPFQVIETVNESRA
                     TRETEKKVGGSLFDIYHGKEGDTFEAGWKNKLIWGDNLLVLGSLLEKFAGKIDLIYID
                     PPFATGADFSFTAEIGDSGKEILKEQSAIEEKAYRDTWGAGLRSYLTMMRDRLTIMRE
                     LLADHGSMVVHCDFRVDSHLRMIMDDVFGPGRYTNQIVWNYATGGASKEFFACKHDIL
                     LYYRKSDKYKFYPDRVREQRTEKALQRAQNPIGARISADDTTKLPTDVWQIPALNPMA
                     VERLNYPTQKPEDLLTKVLLATSDPGNLVMDIFCGSGTTLAVAEKLGRRWIGCDLGRW
                     GIHVSRKRLLGIDNCKPFEILNLGKYERQYWQGVTFAENGKTLTERALYEYLAFVLKL
                     YKAQPVAGMDHLHGKKSRAMIHIGAVDAPVTIAEIDAALDECVNVKQPELHILGWEWE
                     MGLYDLMVGAAKQKGVKLMLLQIPREVMEQQAADKGDVRFFELAHLEAEIKQSKNLEA
                     KVTLKDFVIPNTELIPEEVREKVKKWSDYIDYWAVDWDFQNDTFMQGWVAYRTRKERK
                     LSLTSDTHTYNKAGKYRILVKVIDIFGNDTSQAFEVEVK"
     gene            5564..8347
                     /locus_tag="PLL14_01360"
     CDS             5564..8347
                     /locus_tag="PLL14_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG02593.1"
                     /translation="MPNAIIAYDKDLPEIPGRRPWEKPTSFLVKDANSPTGWREDSSG
                     RRASTMLLVPKIRDAVDKWRAEGYPGASDVALRLFSYWFEEDHEVKGFSLPFRYYFCQ
                     REAIETMVWLVEIAKQKDAKKLIQSYATVFQKDLLSQNIEFQKTADGQRQIVRYVPEQ
                     DAEGVQDLPPENLRRFAFKMATGSGKTWVMAMAMVYSRFHKLMVLKSELSTNFLIVAP
                     NVIVYQRLARDFASNQIFHDLPLIPPEWRGQFSQKVILRGEAGEPDPSGNLFLANIQH
                     LYESRDQAWTPQNAIDALLGKKPNKDLAASGQRSMLERVKSLKDIVVINDEAHHVHDD
                     DLAWSQSLLAIHESLPKGLSMWLDFSATPKDQRGMYFPWTICDYPLAQAVEDRIVKAP
                     IIVTKEDDPKSPAKEPENISQGNVVKKYSYWIQAAVTRWKEHHQVYKKLKIQPVLFIM
                     AEKTAYADVIGKYLWETPEFGLKESEVLIIHTDTKGEITKADLDKAREAARDIDKADN
                     KVKAIVSVMMLREGWDVKNVTVVLGLRPFTADAQILPEQVVGRGLRLMSKFQVGPDQI
                     QTLEVLGTRKLLDMLRTTLEAEGVGVASTKTDPPKPVIIEALDARMKYDIAIPLTKPS
                     LSHDIRKIADLKVSSLEAIFDQKELMEVYRLHLKMEFATTETEVHQADIATEHLMAAQ
                     EILVKITNKIISQAGLPNRFADLYPLVRDYVVTRCFGRLIDLEDKAVQSNLDRYELQE
                     GIVKYLARKIAELTVAPRVIEFDRQDFRLSQTSPFCWRRDLPALKAEKTVFNFVATFN
                     KYERRFAEFLDKAKDIRRFASLGTTEQGDSGTQFRIDYMKPSGAIGFYYPDWVAVQKT
                     KDGEVNWIIETKGRVWVGTEAKDEAITRWCNRVSEATATNWKFVRVNQIVFDKTRWTS
                     LQQLIEQIQATLA"
BASE COUNT         1953 a         2396 c         2434 g         1657 t
ORIGIN      
        1 aatgggcctg tttttccaca gtgtggaaaa tatttttcca tagcgtggaa aaatcacgaa
       61 aaagttttcc atactgtgga aaacttgatg gtttgcgtgg gctttcggct ggtattcgcg
      121 ggcaggcatt gctagattct aaactgtttt gaaggcgcaa gcagacagca gggcaaaagc
      181 acagccgggg gaacggcccg agacgctcca ctatcatttg caccgccagc gccttcggga
      241 ccgcttcgag aaggccgggt ttgccggact ggcggagcac gaagtcgtcg aactgctcct
      301 gacgctggcc atcccccgcg tggatgtgaa ggagcaagcg aaggctttgc ttcgacggtt
      361 cgggaatctc aagggcatca tggatgcgcc catcggagag ttgcgcgggg cagccaaaat
      421 cggggcggtg gcccccgtcg ccatcaagat catcaaagaa ttggcgacgc tctatcttca
      481 acaagacgcg gagggcagcg attgcctcgc ggagcccgcg aaactcgaaa acctctggcg
      541 gatgcgcata ggcggactcc ccaacgaagt cttcgaagtc acatatctgg actccgccta
      601 tcgcccctta cgcgacgggg ttgaacggtt ggaggaaggc accatcgacc gggcggcggt
      661 ctatcctcgg aaggttatcg aagccgcctt gaagcggggc gcggccgcca tcgttctggc
      721 gcacaaccat ccgaacggcg ccgttgctcc aaccgaacag gacaaactgc tgacccgcgc
      781 cattgtcttg gcggcggaaa cggtgcgcgt gcgggtgttg gaccatttga tcgtttcgac
      841 gaatgaggct ttcagcttca agaaagccgg gcttttgtga gcatcgctcc cgcgatccga
      901 aagcattatc cgcacctgaa cgacgcacaa cgcgccattg tggaccacga agacgggccg
      961 ttgcttgtga ttgcggggcc cggttccggc aagacccatt gcatcgtttt gcgggcgcta
     1021 aatctgctgg tgcaggagaa ggccacgccc aagcaactcg taatgtgcac tttcactgaa
     1081 aaggctgcat tcgagatgcg ggaccgtctg gcttccgccg cccggaaggt gggctatgcc
     1141 ggggatctct cggaacttac ggtctccacg atccacagtt tctgcaatcg cattctgtcc
     1201 cagcatcgac accggactcc gcttgggcat ggttttgaaa ccttggatga cctgtctcaa
     1261 ctccttttca ttttcgagca gttcgaccaa atcatcggcc cgcagacgga gggattctat
     1321 ctccgtcgct ggagcactcg ctggacggcc atcgaaggcg cgcggaacta cttcggcaag
     1381 atcacggaag aactcgtcga tccggtgcaa ctggtcgccg ccgccgatcc gttcctaacc
     1441 gaaatcggca acgcctatct gcgctacgag cacgccctgc aagaggcaaa ccgcatcgat
     1501 ttcgcgcacc tgcaacgagc cgtgatcgat ctgctcgccg atccggaaac cgccgaagct
     1561 gtgacgcggg acatccgcca tgtcctcgtc gatgaatacc aggacacgaa ccacatccaa
     1621 gaacagttgc tcctgaaact cacggagaaa agtcgcaatc tctgcgtggt gggcgatgag
     1681 gaccagagcc tttatcgttt tcgcggcgcg acggtccgca acatcctgga gtttccgcag
     1741 cggatgccgg gttgcgcggt cgtcaagctc accacgaact accgctcgca tcgagacatc
     1801 gtggagaaat acaaccgctg gatggcgtcg gcggactggc gcaatccgaa cgggcccgcc
     1861 ttccgctacg acaagaccat cgaagcagat gccacgcaga agttcccgga ctatcccgcg
     1921 accttctgca tctggggcaa aaaccagcgc gacgaagtgg cccgctttgc agacatggcc
     1981 gcgtttctaa agaaaaacgg ggtcattcag gatttcaacc agatcgccct gttgcttcac
     2041 agcgtacgcg aggaacacag cgggccctac atcgccgcgc tggaagccaa gggcataccc
     2101 gccttttgcc cgcgggcccg gtcgttcttc ctgatcccgg aaatccgcga tctggttgca
     2161 tgtttcgcgc tcctgttcgg ctggcacggg gacggtcggg gcgaagtcca cggttccatt
     2221 gccgaactcg cggcctatgt ggacgacgcc cttgtccaac tggctcgccg cttcgggggg
     2281 cagcacccgc tggcgaagac cttgcaacgc tgggtcagcg aaatcgcggc gcttcaaggc
     2341 aaggatgccc tagacttccg cccggcggac tacttctacc acttgctggc cctcgacccg
     2401 ttcaagcgcg ccatccacaa cgaaaacatc gcccggaact tggcgatttt ctcccaactg
     2461 ctgaatgtgt tccaaggcta ctaccactac actgtcatca ccgctaagaa ccgcgagtac
     2521 atccggttcc acctgttcaa cagcttcctg cgcctcttgt tcgaaggcgg catcaacgaa
     2581 tacgaggatg cggaccagcc ctttcccaag ggccatgtgc agatcatgac catccatcag
     2641 gcaaaaggtt tggaattccc ggtggtcgtg gtcggctcac tcagcaagca gttgtcgagt
     2701 cccaagaaaa tcgaccgcga ccttggggcc ttctaccacc gagcgccctt cgagcccgag
     2761 gggcgcgtca ccgcgttcga ccggatgcgc ctgcactatg tcgccttttc ccggccgcag
     2821 aaggttctgg tgctcacggc cggcgaggcc ccaaaggact atttcgcgcc catttggcag
     2881 ggcctgccgc aatggcccta tgtgcagacg gagctgctgg ccgcccaacg gttcgagctg
     2941 cgcgagcgca ttccgctcaa gaagacctac agtttcacgg gcgatcttcg gatttacgaa
     3001 acctgccccc ggcaatacca attcttccgc gagtacgatt tcacgccgtc acggtcgtct
     3061 gtgatcttct tcggtttgct ggtccaccag accatcgagg acattcaccg catcgtgctg
     3121 gacggcaagc tcgacaccct caacgacaag cgaattcgtg aaatcttcga tcggacattc
     3181 cggttcatgt gcctgtccga tgtgcggccc atcggcgaga cggccaagga aggcgcgttc
     3241 cgtcaggtaa tggactacta ccgccagaac cgcgaggaaa tgcgccgcgt gatccagacc
     3301 gaggtggatg tctcgcttga gaaagacggc tacatcctca ttgggaaggt ggatcttctg
     3361 cttggcggcg atggcaagct ggaactgttg gacttcaagt catcggctcg gccccggaac
     3421 agccccgatc tgattgccgc ctacgaacgc caactctgca cctatgcgca catcctggaa
     3481 cggcgccaca accgccaggt tgaccggctg ttgctatact ggacggccga gccgcgcaag
     3541 gaggatgccc tgatggtgct gccttacgac cgggcgcgag tcgaggaagc cgggcggtgt
     3601 tttgacaaga ccgttggccg gatacaggcg aaggattttg ccgtctccac accaccggag
     3661 cgaacaattt gcaaggaatg cgacctgcgc atgctatgct gcggcgaagg aatcatcgag
     3721 ggattcaaat aaggagcgaa atcatggcga caatcgacat catcaagacc gaactggtct
     3781 ggccgggaaa atacaacgat gacggcactc tcaaggaggt tccgcgcgtt aacctgcctt
     3841 tccaggttat cgaaaccgtc aacgaaagtc gcgccacccg cgaaaccgaa aagaaggttg
     3901 gaggttccct ttttgacatc tatcatggca aggaaggcga taccttcgaa gcaggctgga
     3961 agaataaact gatttggggt gacaatcttc tggtcctagg ttcgttgttg gagaagttcg
     4021 ccggaaagat agacctcatc tacatcgacc cgccttttgc gacaggtgcg gacttctcat
     4081 tcacagccga gatcggggat tctggaaaag aaatattgaa ggaacagtcg gccattgaag
     4141 agaaggcata tcgcgacaca tggggggctg gccttcgatc gtacctaacc atgatgagag
     4201 accgcctaac gataatgcgc gaactcttgg cagaccatgg cagcatggtg gttcactgtg
     4261 atttccgtgt tgacagtcat ttgcgaatga taatggacga tgtcttcggt cccgggaggt
     4321 acaccaacca aatagtttgg aactacgcga caggcggagc aagtaaagag ttctttgcct
     4381 gcaaacacga cattctcctc tactaccgca aaagcgacaa atacaagttc tatccagatc
     4441 gggtacgaga gcaacgcacg gagaaagcat tgcagcgtgc acagaacccg atcggtgcaa
     4501 gaatcagcgc tgacgacact acgaaattac caacagatgt atggcaaata cctgcgctca
     4561 atcctatggc agtcgaacgc ctaaactacc cgacgcagaa gcctgaagac ctgctcacca
     4621 aggttcttct ggcaacctcg gatcctggca atcttgtcat ggatatattt tgcggatccg
     4681 gcaccacact tgccgtcgcc gaaaagcttg gccgtcgctg gatcggctgc gatctcggtc
     4741 gctggggcat tcatgtgtcg cgcaagcgcc tgcttggaat cgataactgc aagccgtttg
     4801 agatattgaa cctcggcaag tacgaacggc agtactggca aggggttacc tttgcagaga
     4861 acggaaagac gctgaccgag cgggcgctct acgaatatct ggcgttcgtc ctaaagctat
     4921 acaaggccca gccggtcgcc gggatggacc atctgcacgg taagaagagt cgggccatga
     4981 ttcacatcgg agctgtggat gcccccgtga ccatcgccga aatcgatgcc gcgctggacg
     5041 aatgcgtgaa tgttaagcag ccggaactgc acatcctggg ttgggaatgg gagatggggc
     5101 tttatgacct tatggtcggg gcggccaagc agaaaggcgt caaacttatg cttctgcaga
     5161 ttccccgcga ggttatggag cagcaagcgg ctgacaaggg cgatgtgcgg ttcttcgagc
     5221 tggctcacct ggaagcggag ataaaacagt caaagaatct ggaggccaag gtcacgctga
     5281 aagacttcgt gattccgaac actgaattga tccctgagga ggtacgcgaa aaggtcaaga
     5341 agtggtcgga ctacatcgat tattgggccg tggattggga tttccaaaac gacaccttca
     5401 tgcagggctg ggtggcctat cggacgcgca aagagcgcaa gctttcgctg acatccgaca
     5461 cgcacaccta caataaggcg ggcaaatacc gcatcctggt caaggtcatc gacattttcg
     5521 gcaacgatac ttcgcaggcg ttcgaagtgg aggtcaagta gccatgccga atgcaatcat
     5581 cgcctacgac aaggacctgc cggaaatacc ggggcggcgg ccttgggaaa aaccaacctc
     5641 gtttctcgta aaagacgcca atagtccgac gggctggcgg gaagactctt ccggccgccg
     5701 agccagcacg atgctgctgg tgcctaaaat ccgcgatgca gtggataaat ggcgggcgga
     5761 aggctatccg ggggcttccg atgttgcgct gcggctgttc tcgtattggt tcgaggagga
     5821 ccacgaggtc aaaggcttca gcctgccttt tcgttactat ttctgccagc gggaggccat
     5881 cgaaacgatg gtctggctgg tggaaatcgc caagcaaaaa gacgcgaaaa agcttatcca
     5941 atcctatgcc acggttttcc agaaggattt gctgagccag aacatcgaat tccaaaagac
     6001 ggcggacggc caacggcaga tcgtgcgcta tgtgccggag caggatgccg agggcgtgca
     6061 ggacctgccg cccgagaatc tgcgccgctt cgccttcaag atggcaacgg gttcgggcaa
     6121 gacctgggtc atggctatgg cgatggtcta tagccgcttc cacaagctta tggtcctaaa
     6181 gtcggagctt tcgaccaatt tcttgatcgt ggcgcccaat gtgatcgtct atcagcggct
     6241 ggcaagggac tttgccagca accagatttt ccacgatttg ccgctgatcc cgcccgagtg
     6301 gcgcggacag ttttcgcaaa aggtcatcct gcggggcgag gccggcgagc ccgatccttc
     6361 gggcaacctg tttttggcca atatccagca tctttacgaa tcgcgggatc aggcgtggac
     6421 gccgcaaaac gccattgatg cgttgctggg caagaaaccg aacaaggatc tggcggcttc
     6481 cggccagcgg tcgatgctgg agcgcgtcaa gtcgctgaaa gacatcgtgg tcatcaacga
     6541 cgaggcccac catgttcacg acgacgatct ggcctggagc cagtcgctgc tggccatcca
     6601 cgagtcgctt cccaaggggc tttcgatgtg gctggatttt tcggccacgc ccaaggacca
     6661 gcgaggcatg tatttcccgt ggacgatctg cgactacccg ctggcccagg cggtggagga
     6721 ccgcatcgtc aaggccccga tcatcgtcac caaggaggac gatccgaaaa gcccagcgaa
     6781 ggagccggaa aacatcagcc aaggcaatgt cgtaaagaag tacagctatt ggattcaggc
     6841 agccgtcacg cgctggaagg aacatcacca ggtctacaag aagctaaaga ttcagcccgt
     6901 gctgttcatc atggcggaga aaacagccta tgccgatgtt atcggcaagt atctgtggga
     6961 aactccggaa ttcggcctca aggaatcgga agtgctgatc atccataccg acaccaaggg
     7021 cgaaatcacc aaggcagacc tggacaaggc ccgagaggcg gcgcgagaca tcgacaaggc
     7081 cgacaacaag gtcaaggcca tcgtcagcgt gatgatgctg cgcgagggct gggatgtgaa
     7141 gaatgtcaca gtggttttgg ggctacggcc cttcacggcg gacgcccaga tcctgcccga
     7201 gcaggttgtc gggcgcggcc tgcggctgat gtctaagttc caggtggggc ccgaccagat
     7261 ccaaacgctg gaagttctcg gcacgcgcaa gttgctcgac atgctgcgca ccaccttgga
     7321 ggccgagggc gtcggcgtgg cgtccaccaa gaccgacccg cccaagcccg tcatcatcga
     7381 ggccctggac gcgcggatga aatacgacat cgcgattcca ttgaccaaac cgagcctttc
     7441 gcacgacatc cgcaagattg ccgacctgaa agtctcttct ctggaggcca tcttcgacca
     7501 gaaggagctg atggaggtat accggcttca tttgaagatg gagtttgcga ctaccgagac
     7561 cgaagtgcac caggctgaca tcgcgacgga gcacctcatg gcggcgcaag agattttagt
     7621 aaaaatcacc aataaaatca tcagtcaggc ggggttgccg aaccgctttg ccgatctcta
     7681 tccgctggtc cgggattatg tcgtcacgcg gtgctttggc cgcctaatcg atcttgaaga
     7741 caaggcggtt caatccaatc tggaccggta tgaattgcag gagggcatcg ttaaatatct
     7801 cgcccgcaaa attgccgaat taacggtcgc gccccgcgtg atcgagttcg atcgtcagga
     7861 cttccggctt tcgcagacca gtccgttttg ctggcgccgg gatctgccgg cattgaaagc
     7921 agaaaaaacc gttttcaact ttgttgccac attcaataag tacgagcgta ggtttgccga
     7981 gtttctggac aaggccaagg atattaggcg tttcgcctcc ttgggcacaa ccgagcaggg
     8041 cgattccggc actcaattcc gcatcgacta catgaagccc agcggcgcaa tcggcttcta
     8101 ttatcccgat tgggttgccg tacagaaaac caaggatggc gaggtcaact ggatcatcga
     8161 aaccaagggc cgcgtgtggg taggcacgga ggccaaggac gaggccatta cccgctggtg
     8221 caatcgcgtc agcgaggcca cggccaccaa ctggaaattt gtacgagtaa atcagatagt
     8281 cttcgacaaa acccgctgga ccagccttca gcagttgatt gagcagattc aggccacgct
     8341 ggcttgatcc ataatccggt ttaagacttc gcaagattaa accggaagac gcgcgcgccc
     8401 ccacccgaag cccgcagggc ggaagagcgc ggcagaacgc
//
LOCUS       DAOSIT010000026         8409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7895_length_8409_cov_9.353005, whole genome shotgun sequence.
ACCESSION   DAOSIT010000026 DAOSIT010000000
VERSION     DAOSIT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7895_length_8409_cov_9.353005"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /gene="truB"
                     /locus_tag="PLL14_01365"
     CDS             complement(<1..246)
                     /gene="truB"
                     /locus_tag="PLL14_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767422.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG02594.1"
                     /translation="MTARKAWQRVDGVLLLDKPTGMSSNAALQAARRLFSAAKAGHTG
                     TLDPLASGLLPLCFGEATKFSVDLLAADKTYEAELQFG"
     gene            complement(248..625)
                     /gene="rbfA"
                     /locus_tag="PLL14_01370"
     CDS             complement(248..625)
                     /gene="rbfA"
                     /locus_tag="PLL14_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG02595.1"
                     /translation="MATNRGFSRRDRIAEQIRRELAEVIRTELRDPRVGMISLTDVQV
                     SPDYAHAKIFFSSLAGSDTLVSVQEGLEKAAGFLRSELGKRISIHMTPQLHFVFDESL
                     ERGAQLSKLISEAVAISDQDHQA"
     gene            complement(626..3571)
                     /gene="infB"
                     /locus_tag="PLL14_01375"
     CDS             complement(626..3571)
                     /gene="infB"
                     /locus_tag="PLL14_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG02596.1"
                     /translation="MAQTSVAQFATQLKMPAGTLLEQLQKAGVAKKQADDLLSEQDKS
                     RLLEYLRRSHGPADAKTKITLTRKETTEIRSHDAHGKSHTVQVEVRKKRVLVKRDVPD
                     VRAERPPGQPLPAVPEAVVVAAEAVAETRVEAVVETRVEPAAEVKTAPPSEEKAVPLV
                     EAKAAPMVEVKAAVPVVEARAVPAVEVKTVPPIDVRGKGKGKATADAAVRGATAETRI
                     EPVAAPDAKTGAKKVVTRASIIGEEQQRLRAEEERRNAELRALQEAEFKSKQQRSADL
                     ARLKQDAEEKAIAAKAAGVAKQVAAQTASDQPTEDKTLHKPVAKPATTDKKVPDKKAP
                     EKKVADKKGQWKSEGANKRPGLKTRGATGGTGWRDNKHGKRAGRGGGDEDEQHSFQLP
                     VDPVVHQVYVPETISVADLAHKMAVKATEVIRALMKMGSMVTINQALDQETAMIIVEE
                     MGHKAFAAKLDDPDAFLDDSSAEQKDVPLESRAPVVTVMGHVDHGKTSLLDYIRRTRV
                     ASGEAGGITQHIGAYHVQTDRGMVTFLDTPGHEAFTAMRARGAKATDLVILVVAADDG
                     VMPQTREAIHHAKAAGVPIVVAVNKIDKPGAQPERVKQELVGEQVVPEEYGGDAPFVS
                     VSAKTGVGIDELLENVLLQAEVLELKAPRKAPAKGLIIEARLDRGRGPVATMLVLSGT
                     LRQGDVLLAGQVFGRVRAMLDENGKVIKEAGPSIPVEILGLSDVPAAGQEAVVLADER
                     KAREIALFRQGKYRDVKLATKQAANLESILDQMTEAEAKVLALIIKADVQGSQEALVQ
                     SLQKLSTGEVKVNVIHAAVGAISESDVHLAQASKAVIIGFNTRADAGARKAAETAGVD
                     IRYYNIIYDAVDEVKAALSGMLSPEKREDITGLVEIRQVFRATRIGTIAGCYVLEGVI
                     KRSSRARLLRNHIVVWDGEIESLKRFKDDVREVRGGFECGLSLKNFSAYEEGDQLEVY
                     DVTEVARTL"
     gene            complement(3591..5063)
                     /gene="nusA"
                     /locus_tag="PLL14_01380"
     CDS             complement(3591..5063)
                     /gene="nusA"
                     /locus_tag="PLL14_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOG02597.1"
                     /translation="MSREILLLVDVLAREKNVTKDIVFGALEMALASATKKRIHDDAN
                     VRVAVDRETGDFETFRRWEVVADQDFLDEEQQVPLSEAQAQDPEVEIGDYLEEALEPI
                     DFGRIGAQAAKQVILQKIRDAEREQVLSDFLGRKEHLVTGTIKRMERGNAIIETGKME
                     ALLPRDQMIPRENLRIGDRVKAYLLRIDRSARGPQLVLSRTAPEFIIQLFALEVPEVD
                     DGLLEIKAAARDPGMRAKIAVKSNDQRIDPIGTCVGMRGSRVTAVTNELAGERVDIVL
                     WSADPAQFVVGALAPAEVSSIIVDEEKHSMDVVVDEQNLAIAIGRGGQNVRLASEMTG
                     WTINLMTEEESKTLVEAEAAAIRVLFMEKLDVDEDVANILIDEGFSTLEEVAYVPLHE
                     MLEIEAFDEATVQELRERARNVLLTDAIVTEEKIGDVAEDMLTLEGMDKSLAGKLAGS
                     GIKTRDDLADLAVDELTEITGIDAQRAKQLITTARAHWFE"
     gene            complement(5078..5545)
                     /gene="rimP"
                     /locus_tag="PLL14_01385"
     CDS             complement(5078..5545)
                     /gene="rimP"
                     /locus_tag="PLL14_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOG02598.1"
                     /translation="MDMHEVLEKTVTGLGYELVDVERSPHGRVLRVFIDKPDKPRGVD
                     VEDCALVSNQLSRVLTVENIDYDRLEVSSPGIDRPLKKATDFERFAGSEITLKLRLPL
                     NGRRNFSGVLHGIQDGRLRLQTDAGEMEVDLVNIDKARLVPKFDMLKSGDRLV"
     gene            complement(5676..6647)
                     /locus_tag="PLL14_01390"
     CDS             complement(5676..6647)
                     /locus_tag="PLL14_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG02599.1"
                     /translation="MPNNRRRPPADPPRSPLSSASPAAAGGRRRPPAPASSLAADNSR
                     HALRTTTGESSRRSRHHEPVGKPERLHKLLANSGIGSRREMEALIGAGRIMVNGEPAH
                     IGQTVSPGDRIKVNGKLVQLRFSDRLPRVILYHKPEGEIVSRNDPDHRPNVFTSLPRM
                     SAGRWVAVGRLDFNTSGLLLLTTSGDLANRLMHPRYQLVREYAVRILGELPDEARLRL
                     LEGIELEDGPAKFSSFQEAGGEGANRWYRVSLFEGRNREVRRMFEAVGVVVSRLMRVR
                     YGPFLLPPGLKRGQVQELPEAEVKKLLADFGMATAQPERPARRSRER"
     gene            complement(6637..7224)
                     /gene="scpB"
                     /locus_tag="PLL14_01395"
     CDS             complement(6637..7224)
                     /gene="scpB"
                     /locus_tag="PLL14_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG02600.1"
                     /translation="MSVATSDNSLPANAPEAPDEWKRILEAVLLSAREPLSLVELRKV
                     FADRVAGEVLHALLDELRAEWRERPLELLQVASGWRFRTRAEYMPYLERLNPEKPPRY
                     SRAVLETLAIIAYRQPVTRGDIEDIRGVTVATQVIRVLEERGWIDVVGHRDTPGRPAL
                     LATTRKFLDDLGLRSLAELPALEQINPTLDLANAQ"
     gene            complement(7202..8047)
                     /locus_tag="PLL14_01400"
     CDS             complement(7202..8047)
                     /locus_tag="PLL14_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOG02601.1"
                     /translation="MSDTASVEEAAAAPLDSVVSPLAMIYGQPLAQFPRDLYIPPDAM
                     AVMLDAFEGPLDLLLYLIRKANVNVLDIPMALLTEQYLVYVEAMRSQNLELAADYLVM
                     AAMLIEIKSRMLLPRPKAATGDETEDPRAELVRRLIEYEQMKLAAQQINAWPQAERDF
                     DWVEVWVEKALLRRLPEVSPLDLQNAWHGILRQARLHTRHRVEREELSVREHMGVILR
                     RLREAGGFVDFVDLFDPTQGVPVLVVHFLAVLELAREHLLEMTQVEVFAPIYLRLTDE
                     RSDFR"
     gene            complement(8074..>8409)
                     /locus_tag="PLL14_01405"
     CDS             complement(8074..>8409)
                     /locus_tag="PLL14_01405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG02602.1"
                     /translation="MPTDPARIRRTDPGEPANCPVWQLHQVYSDETCQAWVEQGCRSA
                     GIGCLDCKQPVIDGILREQAPMRARAQPFLEDPKLVREIIGDGNSKARCLARETMRDV
                     RQAIGLDFN"
BASE COUNT         1505 a         2795 c         2387 g         1722 t
ORIGIN      
        1 cccgaactgg agttcggctt cataggtctt gtcggccgcg agcaggtcga ccgagaactt
       61 ggtggcttca ccgaaacaaa gcggcagcag accgctagcc agaggatcga gggtcccggt
      121 gtgccccgcc ttggccgcag aaaagagacg ccgtgcggcc tgcaacgccg cgttcgacga
      181 catgccggtc ggcttgtcaa gcagcagcac gccatccacg cgctgccagg ccttcctggc
      241 ggtcaattca ggcctgatga tcttgatccg agatcgccac ggcctcgctg atcagcttcg
      301 acaactgcgc gccgcgttcc agcgattcgt cgaacacgaa atgcagttgt ggcgtcatgt
      361 gaatgctgat gcgcttgccc aactcgctgc gcagaaatcc tgcggccttt tccaggccct
      421 cctgcaccga tacgagtgtg tcgctgccgg cgagactact gaagaagatt ttggcgtgtg
      481 catagtccgg actcacctgc acatcggtca gactgatcat cccgacgcgc gggtcccgga
      541 gttcggtccg gatcacctcg gcaagttcac ggcgaatttg ttcggcaatc cggtcccgtc
      601 gggaaaaccc cctgttggta gccacttaca gcgtcctggc aacttccgtg acgtcataca
      661 cttcgagttg gtcgccctcc tcgtaggcac tgaaattctt cagtgagagg ccgcattcaa
      721 agcccccgcg aacctccctg acgtcatcct tgaaacgctt caaggactcg atttcgccat
      781 cccagacgac gatgtggttg cgcagcagcc gcgcacggga acttcgcttg atgactcctt
      841 caagcacgta gcagccggcg atggtgccga tgcgggtggc gcggaacacc tgcctgatct
      901 cgaccagacc agtgatgtcc tcccgcttct cgggtgaaag catgccggac agtgcagcct
      961 tcacttcatc caccgcatcg taaatgatat tgtagtagcg gatatcgacg cctgcggttt
     1021 ctgccgcctt gcgtgcaccg gcgtcggcac gcgtattgaa gccgatgatg accgccttcg
     1081 aagcctgcgc cagatggacg tcggactcgc tgatcgcgcc caccgccgcg tgaatgacgt
     1141 tgaccttgac ttcgccggtc gacaacttct gcagggactg caccagggct tcctgggagc
     1201 cctgtacgtc ggccttgatg atcagtgcca ggaccttggc ttccgcttca gtcatctgat
     1261 cgagaatgct ctcgagattc gctgcctgct tggttgccag tttgacatcg cgatacttgc
     1321 cctgacggaa cagggcaatc tcgcgcgcct tgcgctcgtc cgccaatacc acggcttcct
     1381 gcccggcagc cgggacatcc gagagaccca ggatttcgac cggaatcgac ggaccggcct
     1441 ccttgatgac cttgccgttt tcgtcgagca tcgcccgtac ccgaccaaac acctgaccgg
     1501 caagcaacac gtcaccctgg cgcagcgttc cggacagaac cagcatcgtg gccactggcc
     1561 cgcgccctcg gtccaggcgg gcctcgatga tcaggccctt ggccggcgcc ttcctcggcg
     1621 ccttcaattc cagtacctcg gcctgcagca ggacgttttc gagcagttcg tcgataccta
     1681 cgcccgtctt ggccgagaca ctgacgaagg gggcgtcgcc gccatattcc tccggcacca
     1741 cttgttcgcc aaccaactcc tgtttcaccc gctcgggctg agctcccggc ttgtcgatct
     1801 tgttcactgc caccacgatc ggcactccgg ccgctttcgc atggtgaatc gcttccctgg
     1861 tctgcggcat cacgccgtcg tcggcagcca ccaccagaat gaccaggtca gtggccttgg
     1921 ccccgcgcgc gcgcatggcg gtgaatgcct cgtgacccgg cgtgtcgaga aaagtcacca
     1981 tgccgcggtc ggtctgcaca tgataggcac cgatgtgctg ggtaatgcca cccgcctcgc
     2041 cactggcgac gcgggtacgg cgaatatagt cgagcaagga agtcttgcca tggtcgacgt
     2101 gtcccatcac cgtgaccacc ggggctcgcg actcaagcgg aacgtctttc tgctcagcgg
     2161 agctgtcatc caggaaggca tcggggtcgt cgagcttggc cgcgaaagcc ttgtgaccca
     2221 tttcttcgac gatgatcatc gccgtttcct gatcgagtgc ctgattgatg gtcaccatcg
     2281 agcccatctt catcagcgcc ctgatcactt cggttgcctt gaccgccatc ttgtgcgcca
     2341 agtcggcaac tgagatggtt tcgggaacgt acacctgatg gaccaccgga tcgaccggca
     2401 actggaagga atgctgctcg tcctcgtcgc cgccgccgcg acccgctcgc ttgccatgct
     2461 tgttgtctcg ccagccagtg ccaccggtcg ctccgcgcgt cttcagtccg ggccgcttgt
     2521 tggccccctc actcttccac tggccttttt tgtctgccac cttcttttcg ggcgctttct
     2581 tgtccggcac cttcttgtcg gtggtcgctg gcttggccac tggcttgtgc agcgttttgt
     2641 cttccgttgg ctgatcactc gccgtctgcg cggcaacctg cttggcaacc cctgcggcct
     2701 tggcggcgat cgccttttct tccgcatcct gcttcagtcg tgcgagatcg gcgctgcgct
     2761 gttgcttgct cttgaactcg gcctcctgca gggcacgcag ttcggcgttg cgccgctctt
     2821 cctcggcgcg cagtcgctgc tgttcctcac cgatgatcga ggcgcgcgtc accaccttct
     2881 tggcgcccgt cttggcatca ggtgccgcga ctggctcgat tctcgtttcg gcagtagcgc
     2941 cacgcaccgc ggcgtcggca gttgccttgc ccttaccctt gcccctgacg tcaatcggcg
     3001 gcacggtttt cacttcgacg gcgggcacag cccgggcttc gaccacgggc actgctgcct
     3061 ttacttcgac catcggtgct gccttggctt caaccagggg cacagctttc tcttcgctgg
     3121 gtggcgccgt cttgacttca gcagccggct cgactctcgt ttcgaccacg gcttcgactc
     3181 tggtttcagc caccgcctcg gcagcaacga ccaccgcctc gggcactgcc ggaagaggct
     3241 gcccgggggg gcgttcggca cgcacatcgg ggacatcccg tttgacgaga acgcgcttct
     3301 tgcgcacctc gacctgcacc gtatgcgatt ttccgtgcgc gtcgtgagat ctgatctccg
     3361 tcgtttcctt gcgtgtcagg gtgatcttgg tcttggcgtc cgccggccca tgcgatcgac
     3421 gcaaatattc cagcaacctg gatttgtcct gctcggacag cagatcgtct gcctgcttct
     3481 tggccacacc agctttctgc aattgctcga gcaacgtccc agccggcatc ttgagctggg
     3541 tcgcaaattg ggcaacactt gtttgcgcca tatgaattcc tctttcccgc ttactcgaac
     3601 cagtgcgcac gcgccgtggt gatcagttgt ttggcccttt gggcatcaat gccggtgatc
     3661 tcggtaagtt cgtcgacggc cagatcggca agatcgtcgc gcgtcttgat gccactacca
     3721 gccagcttac cggccagtga cttgtccatc ccttcgagag tcagcatgtc ctcggcgacg
     3781 tcgccgatct tctcctcggt gacgatcgcg tcggtcagca gaacattacg tgcccgctcg
     3841 cgcagttcct gaaccgttgc ttcgtcgaag gcctcgatct cgagcatttc gtgcagcggc
     3901 acataggcca cttcctcaag ggtcgagaag ccttcgtcga tgaggatgtt ggcgacatcc
     3961 tcatcgacgt cgagtttctc catgaacagc acacggatcg ccgcggcttc ggcctcgacc
     4021 agagtcttcg actcttcctc ggtcatcagg ttgatcgtcc agccggtcat ttcagacgcg
     4081 aggcgaacgt tctgaccccc acgaccaatg gcgattgcca gattctgctc gtcaaccacc
     4141 acgtccatgc tgtgcttctc ctcgtcgaca atgatcgagc tgacttcagc cggcgccagc
     4201 gcgcccacga caaactgtgc cgggtcagcc gaccagagga cgatatccac acgttcgcca
     4261 gccagttcat tggttaccgc agtcacccgg gaaccgcgca tcccgacaca ggtcccgatg
     4321 ggatcgatac gctggtcatt ggacttgacc gcgatcttcg cacgcatacc ggggtcgcga
     4381 gccgcggcct tgatctccag caggccatca tccacttcgg ggacttccaa ggcgaacagt
     4441 tgaataatga attccggcgc ggtacgcgag aggaccagtt gtggtccacg cgccgagcgg
     4501 tcgatgcgca acagataagc cttgacacga tcaccgatgc gcagattctc tcgcggaatc
     4561 atctgatcgc gcggcaacaa ggcttccatc ttgccggttt cgatgatcgc gttgccacgc
     4621 tccatgcgct tgatcgtgcc agtcaccaga tgttccttgc gacccaggaa atcgctcagc
     4681 acctgttccc gctcggcatc gcgaatcttc tgcagaatca cctgcttggc cgcctgcgca
     4741 ccgatccgac cgaagtctat gggctccaga gcctcttcca ggtaatcccc gatctcgacc
     4801 tcgggatcct gcgcctgcgc ttccgacaac ggcacctgct gctcctcgtc aaggaagtcc
     4861 tgatcggcaa cgacctccca gcgacggaag gtctcgaaat cgccggtttc gcgatcaacc
     4921 gcaacgcgga cgtttgcgtc gtcgtggata cgcttcttgg tagccgacgc cagcgccatt
     4981 tccagcgcgc cgaagacgat atccttggtc acgttctttt cacgtgccag gacatctacc
     5041 agcagcaaga tttcgcggct catttcctca ttacctccta aaccagcctg tcgcccgatt
     5101 tcagcatgtc gaacttgggc accagccgtg ccttgtcaat attgaccagg tcgacctcca
     5161 tttcgcccgc atcggtctgc agacgcagcc tgccatcctg aatgccgtgc agtacgccgc
     5221 tgaagttgcg ccggccattc aacggcaggc gcaatttcaa tgtgatttcg gaacccgcga
     5281 aacgctcgaa atcggtggct ttcttcaacg gcctgtcaat ccccggcgag gagacttcca
     5341 gccggtcata gtcaatgttc tcgaccgtca gcacgcgtga gagctgattg ctcaccagag
     5401 cacaatcctc cacatcgacg ccgcgcggct tgtccggctt gtcgatgaac acccgcagca
     5461 cgcggccgtg cggacttctt tcgacgtcta ccagctcata gccgagaccc gttaccgtct
     5521 tttcaagaac ttcgtgcata tccatggctg caaacaaaaa atgggcgaaa cgcccatccc
     5581 cgaaaaaatt gccccctggg gggcgagcgg aaaacccttg gattataacg aaaatatcag
     5641 caaaggtaaa tcggcctgcg atcaatcgcg atacttcaac gttctcgcga ccgccgcgcc
     5701 gggcgctcgg gttgcgctgt cgccatcccg aagtcagcca ggagcttctt cacttccgcc
     5761 tcgggcagtt cctggacctg accccgcttc aagccaggcg gcagcaggaa ggggccgtag
     5821 cggacccgca tcaggcggct gacgacgaca ccgacggcct cgaacatgcg ccgcacctca
     5881 cggttgcgcc cctcgaacag cgatacgcgg taccagcgat tggccccctc gccgccggct
     5941 tcctgaaagc tcgaaaattt tgccgggccg tcctcgagtt cgataccctc cagcaggcgc
     6001 agtcgtgctt cgtcgggcaa ttcgccaagg atccgcaccg cgtactcacg caccagctga
     6061 taacgtggat gcatcagccg attggccaga tcccctgaag tggtcaacag cagcagccca
     6121 ctggtgttga aatcgaggcg gccgacagcc acccagcgcc cggccgacat ccgtggcaga
     6181 gaggtaaaca cgttcggccg atgatccgga tcattgcgcg aaacgatttc gccttccggt
     6241 ttgtgataaa ggatcacccg cggcaggcgg tcggagaagc gcagttgtac cagcttgcca
     6301 ttgaccttga tccggtcgcc cggactcacc gtttggccga tgtgtgccgg ctcaccgttg
     6361 accatgatgc ggccggcacc gatcagcgcc tccatctccc gacgcgagcc aatgccgcta
     6421 ttcgccagca gtttgtgcag gcgctccggc ttgccgacgg gttcatggtg acggctacgc
     6481 ctcgacgact ccccggtagt ggttcgcaac gcatgccgcg aattgtcggc cgcgagcgaa
     6541 gacgcaggcg caggcggccg ccgtcggcct cctgccgctg cgggcgatgc cgacgacaaa
     6601 ggcgaacgag gagggtcggc aggaggtctg cgtcggttat tgggcatttg cgaggtccag
     6661 ggtgggattg atctgttcga gcgcgggcaa ctcggcgaga ctgcgcaaac cgagatcgtc
     6721 gaggaatttc ctggtagtcg ccagcaaggc tggtcggcca ggcgtatcgc gatgaccgac
     6781 gacatcgatc cagccgcgct cttcgagtac ccggatcacc tgtgtggcga ccgtcacacc
     6841 gcggatgtcc tcgatgtcgc cacgcgttac tggctggcga taggcaatga tggccaaggt
     6901 ttccagcacc gctcgcgagt agcgcggcgg cttttcaggg ttcagccgct ccaggtaggg
     6961 catatactcg gcacgcgtcc ggaaacgcca gccgctcgcc acctgcagca gctcgagcgg
     7021 acgttcgcgc cattccgcgc gcaactcgtc gagcagtgca tgcaggactt caccagcgac
     7081 ccggtcggcg aacacctttc gcagttcgac cagcgacagc ggctcgcggg cgctcagcaa
     7141 caccgcttcg agtatccgct tccattcgtc gggagcctcc ggcgcattgg ccggcagcga
     7201 gttatcggaa gtcgctacgc tcatcggtca acctcaggta gatcggagcg aacacctcga
     7261 cttgtgtcat ttcgagcagg tgctcgcgcg ccagttcgag caccgccagg aagtgcacga
     7321 ccagcaccgg aaccccctgc gtcggatcga aaagatcgac gaagtcgaca aagcctccgg
     7381 cctcgcgcag acgacgcagg atcacgccca tatgctcgcg taccgacaac tcctcgcgct
     7441 ctacccgatg gcgggtatgc agtctggcct ggcgaagaat gccatgccag gcgttctgca
     7501 gatccagcgg actgacctcc ggcaggcgcc gcagcagggc cttctcgacc cagacctcga
     7561 cccagtcaaa atcacgctcc gcttgcggcc acgcattgat ttgctgcgcc gccagtttca
     7621 tctgctcgta ctcgatcagg cgccgcacca gttccgctcg cggatcctcg gtttcgtcgc
     7681 cggtcgctgc tttcgggcgg ggaagcagca tccgcgactt gatctcgatc agcatggcag
     7741 ccatcaccag atagtcggcg gcgagttcga gattctgcga acgcatcgct tccacgtaaa
     7801 ccagatactg ctcggtcaac agcgccatcg gaatatccag tacattcacg ttggccttgc
     7861 ggatcaggta cagcagcagg tcgagcggcc cctcgaaggc atcgagcatg accgccatgg
     7921 catcgggcgg gatgtacagg tcacgcggaa actgcgccag cggctgcccg tagatcatgg
     7981 ccagcggact gaccacactg tccaggggcg ccgccgctgc ctcttcgacg ctggcggtat
     8041 cgctcatcgg caatcgcaga acggcaccgg cgctcagttg aagtccagcc cgatcgcctg
     8101 gcgtacgtcg cgcatcgttt cgcgggcgag gcaacgcgcc ttgctgttgc catcgccaat
     8161 gatctcgcgc accagtttcg gatcctccag aaatggctgc gcacgcgcgc gcatcggcgc
     8221 ctgctccctg aggatgccgt cgatcaccgg ctgcttgcag tcgaggcaac cgataccggc
     8281 gctgcgacag ccctgctcca cccacgcctg acaagtctcg tcggagtata cctggtgtaa
     8341 ttgccagacc ggacagttgg ccggctcacc ggggtcggta cggcggatgc gtgccggatc
     8401 ggtgggcat
//
LOCUS       DAOSIT010000027         8386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7927_length_8386_cov_9.654183, whole genome shotgun sequence.
ACCESSION   DAOSIT010000027 DAOSIT010000000
VERSION     DAOSIT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7927_length_8386_cov_9.654183"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2503
                     /locus_tag="PLL14_01410"
     CDS             <1..2503
                     /locus_tag="PLL14_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HOG02603.1"
                     /translation="AALGDQADGPDDAFEVALLDANSGVSVLGGTGLGHSDAIINRQA
                     DGSEYRSDGISVLTNPDGSRSYRLDLSGVAAGTVLNLAFDLIGFGKGADAGSSRVSIR
                     DLHLGSEPARPEAMDDAVITDEDRPLLIDALANDRNARQPGFAPVVVDGPVHGELVVN
                     ADASFRYTPAKDWYGEDRFTYRLSDGQIESGVATVTLTVMPVNDAPLVSPLTATLLED
                     GSVTLDLLSGATDPDGDALIVTAGSPRHGTLSRNDDGSYTYRPAIDYHGEDELAFTIS
                     DGQATTEGTLRLMISAQADAPTLTLGERAGATREIFRSGWESVANTGDTPTPVKGDVL
                     EGWQRLSGGPSMPGGSFQIWSGGDRMRNALGGSLSAYPAAGSGQNWLELNIVGSTPIP
                     TRAIERGIDTIAGATYTLSLTLAGAPGYAAEYTRIGITLDGRNIGSEASTSPLDALAW
                     QTRTFQFVGAGGRQTLRIASEATRFDAWSSGTMIDDIVLSETLPANVGYEDAPMQLPT
                     IAATLRDDDGSEALSIDIAGLPVGATLSDGEHHFTASSLAQRLTISDWDLGKLSITPP
                     PDRTGRFTLTIVATASEQSNGSQASTTASFEVLVLPVDDAPTWTSAPPASFELQAGMA
                     AAGMRLQPSTTSTGVGVTVMTQPITAMPTVNDIGPHRPDAPGARLDMPPQVFRYQAMA
                     RDTDGDALSYRLLDGPAGARIDAASGLLLWETPQAGEQIFRLEADDGKGNRIEQRFTV
                     QFGSALRTGTSGKPPALPGAAGVAAISSRANEQSPRKPGPQNTTPSRIEWGGAQPACF
                     AGARLAQSPWLPEFLGSAKREQSTERAALQIRIMS"
     gene            complement(2647..4050)
                     /gene="fumC"
                     /locus_tag="PLL14_01415"
     CDS             complement(2647..4050)
                     /gene="fumC"
                     /locus_tag="PLL14_01415"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOG02604.1"
                     /translation="MGNSDQTGGEVRAEHDSLGAIDVAATHLWGAQTQRSLKYFAIGR
                     ERMPTEIIHALLLIKRAAAQANSALGEIDAAIAVAIVQAADEILAGQWPDEFPLSVWQ
                     TGSGTQTHMNVNEVLANRASELLGGPRGLGRLVHPNDHVNCGQSSNDVFPTAMHIAGV
                     LLVEQRVLPALDMLAATLRVKAEAFSGIVKIGRTHLQDATPLTLGQEFSGYVEQLRMA
                     RAAIAMTLPGLHALALGGTAVGTGLNAHPDFATRAIAEMARHSGLPFVAAGNYFAALA
                     GHEPLVMAHGALKTLATALSKIANDLRWLGSGPRCGIGELRLPENEPGSSIMPGKVNP
                     TQCEALLMVCAQVFGNDVAISVGAASGNFELNVMKPLIARNFLDSARLLADAMRSFDS
                     YCVCDLQANAARIDELLHGSLMLVTALAPHIGYDRAAQIARRAHAEGTTLRAAAIASG
                     LLSAEAFDQWVVPEDLV"
     gene            complement(4114..5724)
                     /locus_tag="PLL14_01420"
     CDS             complement(4114..5724)
                     /locus_tag="PLL14_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG02605.1"
                     /translation="MKIKTLLWSGFAAICALMAVLVSIGIWQLDVIDKATEDVTRADD
                     NESMARDIAERVNGMRRYQLSALVAADERDQQLERATKAGRENVQQAEALEKKQRNPE
                     TRQIAANMRTLNERYVRSHEQVVALGKEGKLDAMRDLIQGEARSVQRELAAESEKFIK
                     IQSERQAQAEKVMEAAHTLAARLMLGLLVVGVVLGIGIAFLVTRRITGQLGGEPEYAR
                     QLVQRIADGDLSQDLALQSGDTQSLMAYQQQMQLRLRTMLKNIGSTVVSTEGAAQTLA
                     GSSQQVAAASRSASDSAASMAAVVEEMSVSISQVADNARDALKTASHAAELSDSGGGV
                     IEQATSAINRIADTVRQTSGAMRTLDESATRISTVVQVIKEVADQTNLLALNAAIEAA
                     RAGESGRGFAVVADEVRKLAERTGKATNEINSMVLQIQHETRNSLDSMTTAVQQVDQG
                     VDLAGSAGEAIRDIRSSVEQVVAVVNDIGNAISEQSIASQQIAQRVEQVAQASEENNA
                     AAEETASAARTLSQLAGGLRNTVAQFRT"
     gene            6016..>8386
                     /locus_tag="PLL14_01425"
     CDS             6016..>8386
                     /locus_tag="PLL14_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG02606.1"
                     /translation="MASADNPILNSPYDEPARHYATDAQGNLNYRDIRPGRRIFTPDV
                     PQIPIGQPNQGSMFDVNDFRAEYGEHLINRLRDELRAWRQAGYRGVTSRVTRDLLDYW
                     FANPERAAHQKLFFAQQEAVEAAIWLNEVAEKSNTGTHIQNQLRQRQATAGNDPASQL
                     PRIAFKMATGSGKTVVMACLILYHYLNRREYRNDTRYVDYFLILAPGITIRDRLNVLL
                     PDQRNAPPAAARDYYQQRTLVPPAQQHALGELAHRLIITNYHEFLPRILGGNKRTPFD
                     GKLGADGHKVENREDENQMLRRVLGSFKAGRRLLVINDEAHHCYLPRARGRDTELDNS
                     ETENERAAVWFSGLRACARRFQVRVVYDLSATPYYLSGSGFPAYSLFPWVVSDFGLIE
                     AIEAGLVKIPFLPIDDSSQAIDEPVLKNLYENCKTELPRKGQRTQRRETQQANAGEAA
                     PNLPALVRQALDQFYAHYEEYERGLRRRGELKADLFTAPPVFIVVCGNTAVSREVFKE
                     IAGYERVDQSGQTTVVAGRFALFSNFDRDSGRARARPPTLLIDSDALEHSGQIDEGFK
                     KVFAPEIERFKRAYRLRHPERGVDSLTDAELLREVVNTVGKPDTLGAHVRCVVSVAIL
                     SEGWDANTVTHVMGLRAFGSQLLCEQVAGRALRRRYYFTDPKTGKFPPEYAHIIGIPF
                     KLFKAGQSSPVEPPEYATLRALRERDSLEIRFPNLVGYRLQTDSDQLTADFSGTPDYR
                     LDTTRLPVETTLGTAVSEDRQTMRLKLEELRDQTVIYKLTRDYLRRIHGD"
BASE COUNT         1469 a         2988 c         2573 g         1356 t
ORIGIN      
        1 tgccgctctg ggcgatcagg ccgacggtcc cgacgacgct ttcgaagtcg cgctgctcga
       61 cgccaacagc ggcgtgtcgg tgctcggcgg cacaggactc ggccacagcg acgccatcat
      121 caaccgtcag gcggatggta gcgaataccg gtccgatggc atcagcgtgc tcactaatcc
      181 cgatggcagc cgcagttatc ggctggatct gagcggcgtt gccgcaggta cggtgctgaa
      241 cctcgctttc gacctgatcg gtttcggcaa gggcgcggat gccggaagca gtcgggtcag
      301 catccgtgac ctgcaccttg gcagcgaacc ggccaggccg gaagcgatgg acgatgccgt
      361 gatcaccgac gaggaccgcc cgctgctcat cgacgcgctg gccaacgacc gcaacgcgcg
      421 tcaaccaggt ttcgcgccgg tagtcgtcga cggcccggta catggcgaac tggtggtcaa
      481 cgccgatgcg agcttccgct acacgccggc gaaggattgg tatggcgaag atcgcttcac
      541 ctaccggctc agcgatggcc agatcgaatc cggagtcgcc acggtgaccc tcacggtgat
      601 gccggtgaat gatgcgccgc tggtcagccc gctcaccgct acgctgctcg aggatggcag
      661 cgtgaccctc gatctgctgt ccggcgcgac cgacccggac ggcgatgcgc tgatcgtaac
      721 tgccggctcg ccccggcacg gcaccttgag ccggaacgac gacggcagct acacctaccg
      781 cccggcgatc gactaccacg gcgaggatga gcttgccttc acgatcagcg acgggcaagc
      841 gactaccgaa ggcacgctgc gcctgatgat cagcgcgcag gccgatgcgc cgacattgac
      901 cctcggcgag cgcgccggag caacgcggga aatcttccgc agcggctggg aaagcgtcgc
      961 caataccggc gatacgccga cgccggtcaa gggagacgtg ctcgaaggct ggcaacggct
     1021 gagtggcgga cccagcatgc ccggcggcag tttccagatc tggagcggtg gcgaccgcat
     1081 gcgcaatgcc ctcggcggca gcctgagcgc gtacccggct gccggcagcg gccagaactg
     1141 gctcgaactc aacatcgtgg gcagcacgcc gattccgaca cgggccatcg agcgtggcat
     1201 cgacaccatc gccggcgcca cctacaccct ctccctgacc ctcgccggcg cgccgggcta
     1261 cgctgccgaa tacacgcgca tcggcatcac cctcgatggc cggaacatcg gtagcgaggc
     1321 gagcaccagc ccgctcgacg cgctcgcctg gcagacgcga accttccagt tcgtgggcgc
     1381 cggcggccgg cagaccctgc gcattgcctc ggaagccaca cgcttcgacg cctggagcag
     1441 cggcacgatg atcgacgaca tcgtgctcag cgaaacgctg ccggccaacg tcggctacga
     1501 ggatgcgccg atgcaactgc cgacgatcgc cgccacgctg cgcgacgacg acggctcgga
     1561 agccctgtcg atcgacatcg ccggcctccc tgtcggcgcg acgctcagcg acggcgagca
     1621 ccatttcacc gcgtcgagcc tggcgcaacg gctgacgatc agcgactggg atctcggcaa
     1681 actgtcgatc acaccaccgc ccgacaggac tggccgcttc acgctgacca tcgtcgctac
     1741 tgcctccgag cagtcgaatg gcagccaggc cagcacgacg gccagcttcg aggttctggt
     1801 gctgccggtg gacgatgcac cgacgtggac cagcgctcct cccgccagct tcgaactgca
     1861 ggcgggcatg gccgcggccg gaatgcgcct gcagccgagc acgacatcga caggcgtagg
     1921 cgtgacggtc atgacgcagc cgatcaccgc aatgccgacc gtcaacgata tcggccctca
     1981 ccggccggat gcccccggcg cgcgcctcga catgccgccg caggttttca gataccaggc
     2041 catggctcgc gataccgacg gcgacgcgct cagctatcgt ctcctggacg gacccgccgg
     2101 tgcccgcatc gacgctgcca gcggactcct gctctgggag acaccgcagg ccggcgagca
     2161 gatcttccgg ctggaagcgg acgacggcaa gggcaaccgc atcgagcagc ggttcacggt
     2221 gcaattcggc agcgcgctgc gcaccggcac gagcggcaag ccccctgccc tgccaggtgc
     2281 tgccggagta gccgcgattt ccagtcgggc gaacgagcaa agccctcgca agccaggccc
     2341 gcagaacacg accccgtcgc gcatcgagtg gggcggcgcg caacccgcct gctttgccgg
     2401 cgcacgcctg gcgcaaagcc cttggctacc ggaattcctc ggcagcgcga agcgcgagca
     2461 aagcacggag cgtgcagccc tgcagatccg gatcatgtct tgaactggct ggccgaatcg
     2521 gaccggtggt ttctcctgcc tgattgggaa agaccaggcc tacggagacg gcgtcgtcca
     2581 aagatgaaac cgctgcatct tcgccccctt ctcaggcgcg cgtcggcgct gcgataagcg
     2641 tgcgcttcag accaggtctt ccggcaccac ccactggtcg aacgcttcgg cgctcagcag
     2701 gccggaggcg attgccgccg cgcgcagcgt cgtcccctcg gcatgcgcac gcctggcgat
     2761 ctgcgccgcg cggtcgtagc cgatgtgcgg cgccaaagcg gtgaccagca tcaggctgcc
     2821 gtgcagcagt tcatcgatgc gcgccgcatt ggcctgcagg tcgcacacgc aatagctgtc
     2881 gaaactgcgc atcgcgtcgg ccagcaggcg ggcgctgtcc aggaagttgc gggcgatcag
     2941 cggcttcatc acgttcaact cgaagttgcc cgaagcagcg ccgacgctga tcgccacgtc
     3001 gttcccgaaa acctgcgcgc agaccatcag cagcgcctcg cattgcgtcg ggttgacctt
     3061 gccgggcatg atcgaactgc ccggttcatt ctccggcaga cgcaactcgc cgatgccgca
     3121 acgcggaccg ctgcccagcc agcgcaggtc gttggcgatc ttgctcagcg ccgtagccag
     3181 cgtcttcagg gcgccgtgcg ccatgaccag cggctcatga ccggccagag ccgcgaaata
     3241 gttgccggcg gcaacgaaag gcaagccgct gtgacgggcc atttcggcga tcgcgcgcgt
     3301 ggcaaaatcc ggatgcgcat tgagacccgt acccaccgcc gtaccgccga gcgccagggc
     3361 atgcaggccg ggcagggtca tcgcaatggc cgcgcgcgcc atgcgcagtt gctcgacgta
     3421 gccggaaaac tcctgcccga gggtgagcgg cgtggcatcc tgcagatgcg tgcggccgat
     3481 cttgacgatg ccactgaacg cttccgcctt cacccgcagt gtcgccgcca gcatgtcgag
     3541 cgccggcagc acgcgctgct cgacgagcag cacgccggcg atgtgcatcg ccgtcggaaa
     3601 gacatcgttg gacgactgcc cgcaattgac gtggtcgttg ggatgcacca gccggccgag
     3661 gccgcgcggc ccgccgagca actccgaggc acggttggcc aacacctcat tgacgttcat
     3721 gtgggtctgc gtgccggagc cggtttgcca gaccgacagc ggaaattcat cgggccactg
     3781 gccggcaagg atttcgtcgg cagcctgcac gatggccacg gcgatcgccg catcgatctc
     3841 gccaagcgcg ctattggcct gcgccgcggc gcgcttgatc agcaacagcg cgtggatgat
     3901 ctccgtcggc atgcgctcgc ggccaattgc gaaatacttg agggagcgtt gcgtctgcgc
     3961 tccccagaga tgcgtggccg cgacgtcgat cgcgccgagg ctgtcgtgct cggcgcggac
     4021 ctcaccgccg gtctggtcgc tgttgcccac ggctcacact cccgctggca tcaccgcagg
     4081 cgcactgcgc gcggcctgtt gattggtctg cactcaggtc ctgaactgcg cgacggtatt
     4141 acgcaagccg cccgcgagct ggctgagcgt gcgcgccgcg ctggcggtct cttcggccgc
     4201 ggcattgttc tcctcggaag cctgcgcgac ctgctcgacc cgttgcgcga tctgctggct
     4261 ggcgatcgat tgctcgctga tcgcattgcc gatgtcgttg accacggcga ccacctgctc
     4321 gacgctgctg cggatgtcgc ggatcgcctc acccgcggaa ccggccaggt ccaccccttg
     4381 atcgacctgc tgcacggcgg tcgtcatcga gtcgagtgaa ttgcgggttt cgtgctggat
     4441 ctgcaggacc atgctgttga tctcgttggt ggccttgccg gtgcgctcgg cgagcttgcg
     4501 cacctcgtcg gcgacgaccg cgaagccacg ccccgactcg ccggcgcgcg ccgcctcgat
     4561 cgccgcgttc agcgccagca gattggtctg atcggcgacc tccttgatca cctgcaccac
     4621 cgtcgagatg cgcgtcgcgc tttcatcgag ggtgcgcatc gccccggaag tctgacggac
     4681 ggtgtcggcg atgcggttga tggcgctggt cgcctgctcg atcacgccgc cgccgctgtc
     4741 ggacagctcg gcggcatggc tagccgtttt cagcgcatca cgggcgttgt ccgccacctg
     4801 gctgatgctc accgacatct cctcgaccac cgccgccatc gaggctgccg agtcgctggc
     4861 cgagcgcgaa gcagcggcca cctgctgtga cgatccggcc agcgtctgcg ccgcgccctc
     4921 ggtgctgacc acggtactgc cgatgttctt caacatcgtc cgcagtcgca actgcatctg
     4981 ctgctggtag gccatcaggc tctgcgtgtc gccgctctgc agcgcgagat cctggctcag
     5041 gtcgccgtcg gcgatgcgct gcaccagttg ccgggcgtat tcgggctcac cgccgagctg
     5101 gccggtgatc cgacgggtga ccagaaaggc gatgccaatg cccaacacca ccccgaccac
     5161 cagcagcccg agcatcagcc tcgcggccag cgtgtgcgca gcctccatga ctttctccgc
     5221 ctgcgcctgg cgctcgctct gaattttgat gaatttctcg ctttccgcgg cgagttcgcg
     5281 ctgcacgctg cgcgcctcac cctggatcag atcgcgcatc gcatcgagct tgccttcctt
     5341 gccgagcgcg accacctgct cgtggctgcg cacgtagcgc tcgttcagcg tgcgcatgtt
     5401 ggccgctatc tgccgggtct cgggattgcg ctgcttcttc tcgagcgctt ccgcctgttg
     5461 gacattttcc ctgccggcct tggtcgcacg ctccagttgc tggtcgcgct cgtcggcggc
     5521 gaccagcgca ctcagttgat agcggcgcat gccattcacc cgctcggcaa tgtcacgtgc
     5581 catcgattcg ttgtcatcgg cgcgggtgac atcttcggta gccttgtcga tcacgtccaa
     5641 ctgccagatg ccgatgctca ccagcaccgc catcaaggcg cagatcgcgg caaagccact
     5701 ccagagaagc gttttgattt tcattgttag ggcctcttgt caaagaacgg taaggtgcaa
     5761 tcagaaagga attcgtgatt cggtcgcgat tcggcgcagg cgcgcagcag caagtggcac
     5821 gcactttgcg gcatgccaca aacggttgtc aactatcgct tttgccgccc cggcaatacg
     5881 cttggcggat gccattcgag cgcatttcgc caccttttcc ggcgcaattt cacggctttc
     5941 gacgctgccg atacgaagtc aacgagcggc ggcgactgtt agaatgcgcc taccccgcaa
     6001 ccggcgtcac cgcccatggc ctccgccgac aaccccatcc tgaactcccc ctacgacgag
     6061 ccggcgcggc actacgccac cgacgcgcag ggtaacctga actatcggga catccgcccc
     6121 ggccgccgca tctttacccc ggacgtgccg cagataccga tcggccagcc gaaccagggc
     6181 agcatgttcg acgtcaacga cttccgggcg gaatacggcg aacacctgat caatcgcctc
     6241 cgggacgagc tgcgcgcctg gcggcaagcg ggttatcgcg gggtcaccag ccgcgtcact
     6301 cgcgatctgc tcgattactg gttcgccaat cccgagcggg ccgcgcatca gaaattgttt
     6361 ttcgcgcagc aggaagccgt cgaggcggcc atctggttga acgaggtcgc cgaaaagtcg
     6421 aacaccggca cgcatatcca gaaccagttg cggcaacgac aggccaccgc tggcaacgac
     6481 ccggccagcc agttgccgcg catcgccttc aagatggcca ccggcagcgg caagaccgtg
     6541 gtcatggcct gcctgatcct ctaccactac ctcaaccgtc gcgagtaccg caacgacacc
     6601 cgctacgtcg actacttcct gatcctcgcc cccggcatca ccatccgcga ccgtctcaat
     6661 gtccttctgc cggaccagcg caacgcgccg cccgccgccg cccgcgacta ctaccagcag
     6721 cgcacgctgg tgccgccggc tcagcagcac gccctcggcg aacttgccca ccgcctgatc
     6781 atcaccaact atcacgagtt cctgccgcgt atcctcggcg gcaacaagcg cacgcccttc
     6841 gacggcaagc tcggcgccga cggtcacaag gtcgaaaacc gcgaagacga aaaccagatg
     6901 ctgcggcgcg tgctgggcag cttcaaggcc ggacgccggc tactggtgat caatgacgaa
     6961 gcgcaccact gctacctgcc gcgcgccagg ggccgggata ccgaactcga caactccgaa
     7021 accgaaaacg aacgcgctgc ggtgtggttc tccggcctgc gcgcctgcgc ccggcgcttt
     7081 caggtgcgcg tggtctacga tctctcggcg acgccctact acctttccgg atcggggttt
     7141 ccggcgtact cgctgttccc ctgggtggtc agcgacttcg gcctgatcga agcgatcgaa
     7201 gccgggctgg tcaagattcc cttcctgccg atcgacgaca gcagccaggc gatcgacgag
     7261 ccggtcctga aaaacctcta cgagaactgc aagaccgaac tgccccgcaa ggggcagcgc
     7321 acgcagcgcc gcgagacgca gcaagccaac gccggcgaag ccgccccgaa cctgccggcg
     7381 ctggtgcggc aggcgctcga ccagttctac gcccactacg aagaatacga acgcggtctg
     7441 cgccggcgcg gcgaactgaa agccgacctg ttcaccgcgc cgccggtgtt catcgtcgtc
     7501 tgcggcaaca ccgccgtttc gcgcgaagtg ttcaaggaga tcgccggcta cgaacgcgtt
     7561 gaccagagcg gccagacgac tgtcgtcgcc ggtcgtttcg ccctcttctc caacttcgac
     7621 cgcgacagcg gccgcgcgcg cgcccgcccg ccgacgctgc tcatcgacag cgacgccctc
     7681 gaacactccg gccagatcga cgaaggcttc aagaaggttt tcgctcccga gatcgagcgc
     7741 ttcaagcgcg cctaccgcct ccgccacccc gagcgcggcg tcgacagcct caccgacgcc
     7801 gaactgctgc gcgaagtggt gaacaccgtc ggcaaacccg acaccctcgg cgcgcacgtg
     7861 cgttgcgtgg tttccgtcgc cattctcagc gaaggctggg acgccaacac cgtcactcac
     7921 gtcatgggcc tgcgcgcctt cggctcgcaa ctgctctgcg aacaggtcgc cggccgcgca
     7981 ctgcgccgcc gctattactt caccgacccg aagaccggca agttcccccc cgaatacgcg
     8041 cacatcatcg gcatcccctt caagctgttc aaggccggcc agagcagccc cgtcgagccg
     8101 cccgaatacg ccacgctgcg tgcgctgcgc gagcgcgaca gcctcgaaat ccgcttcccc
     8161 aacctggtcg gctaccgcct gcagaccgac agcgaccaac tcaccgccga tttcagcggc
     8221 acgcccgact atcgcctcga taccacccgc ctgccggtcg aaaccacgct cggcaccgcc
     8281 gtctccgaag accgccagac gatgcgtctc aagctcgaag aactgcgcga ccagaccgtc
     8341 atctacaagc tgacccgcga ctacctgcgc cgcatccatg gcgacg
//
LOCUS       DAOSIT010000028         8329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_7998_length_8329_cov_9.426638, whole genome shotgun sequence.
ACCESSION   DAOSIT010000028 DAOSIT010000000
VERSION     DAOSIT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7998_length_8329_cov_9.426638"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /gene="sdhA"
                     /locus_tag="PLL14_01430"
     CDS             complement(<1..1774)
                     /gene="sdhA"
                     /locus_tag="PLL14_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOG02607.1"
                     /translation="MSKLTIPVRKFDAVIVGAGGSGLRAAIQLSEAGLKTAVLSKVFP
                     TRSHTVAAQGGVSASLGNSEEDHWHWHMYDTVKGSDWLGDQDAIEYMCRMAPEVVVEL
                     EHFGMPFDRTDEGKIYQRPFGGHMSNFGEKPVRRACAAADRTGHAMLHALYQRNVRAN
                     TQFFVEWMALDLIRDDKGQVLGVIALEMETGDVVAFHAKATVFATGGAGRIFYSSTNA
                     FINTGDGLGMAARAGIALEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNSANERFM
                     ERYAPNAKDLASRDVVSRAMVTEINEGRGCGPNKDFVLLDITHLDPATIMKRLPGIRE
                     ISIQFAGIDPIKAPIPVVPTCHYQMGGVPTNYKGQVVSQDATPIPGFYAAGEVACASV
                     HGANRLGTNSLLDLLVFGKSSGDSVIDDLRSGAIGLKELPADYAERTLNRLNRLNTQT
                     GGTSVNETRLELQRTMQKHCGVFRFKDMLVEGVAKILDVEKAVLRTEIKDKSQVWNTA
                     RVEALELDNLIEVAKATMISAEARKESRGAHVRDDAPDTAANPNGRDDVNWHKHTLWH
                     RESNRLDYKPVIMKPLSVESIALKT"
     gene            complement(1775..2122)
                     /gene="sdhD"
                     /locus_tag="PLL14_01435"
     CDS             complement(1775..2122)
                     /gene="sdhD"
                     /locus_tag="PLL14_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOG02608.1"
                     /translation="MVNRILVGAHYGLKDWIIQRATAIVMAVYTLIFLVVVGAVGPDT
                     FEAWRGIFANGFMKFATFLFFVSVFYHAWIGIRDIWMDYVKPDGLRLLMMIATAAVLV
                     GYAGWAVQILWRM"
     gene            complement(2116..2508)
                     /gene="sdhC"
                     /locus_tag="PLL14_01440"
     CDS             complement(2116..2508)
                     /gene="sdhC"
                     /locus_tag="PLL14_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOG02609.1"
                     /translation="MAELAIKKKRPKNLDLPTIRLPLPGILSIIHRISGAAMFLLLPF
                     LLWLFQGSLTSPESFAGVKAVVGHPLAKIVLLGLVWFYMHHFCAGIRYLLLDLHKGVE
                     LEAARLSSKIVFVVSIALTLIIGARVLW"
     gene            complement(2627..3364)
                     /locus_tag="PLL14_01445"
     CDS             complement(2627..3364)
                     /locus_tag="PLL14_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG02610.1"
                     /translation="MSRPSSVHSPTFSPLYRQIKELILRDLESGEWGPGDPIPSESEL
                     AGRFGVSQGTVRKAIDEMAAENLLVRQQGKGTFVATHKDPGSFFRFLRLVPNDGDLQV
                     SQSVPLECWRAKAGADVARTLALDTGAPITIVRRLLKLGDEPVVFDEIYLPSELFPDL
                     SLDVLRSGESLYSLFETRYGVRMIRADERLRAVSADRVSAEWLQVADGSPLLLVERVT
                     FTYGHKPVEWRRGFYSTRNYHYHNELG"
     gene            3573..4559
                     /locus_tag="PLL14_01450"
     CDS             3573..4559
                     /locus_tag="PLL14_01450"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOG02611.1"
                     /translation="MSKAPMRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKACQGVIMELEDCAFPLLAGMFATDDPNLAFKDADVCLLVGARPRGPGMERADL
                     LTANGAIFTVQGKAIAENAKEDVRVLVVGNPCNTNALIAGAAARKVGRTNPGNYHGML
                     RLDHNRALSQLAAKTGRPVASFKQLVVWGNHSPTMYADYRNCTSDGDNVKMLVNDPVW
                     NNDVFLPTVGKRGAAIIDARGLSSAASAANAAIDHMHDWVLGSDEWVTMGVPSDGSYG
                     IPTGIVFGFPCECKGGSYTIIQGIEIDDYSREKINKTLKELTDEAEAVQGML"
     gene            4674..5663
                     /locus_tag="PLL14_01455"
     CDS             4674..5663
                     /locus_tag="PLL14_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOG02612.1"
                     /translation="MRHPSQVLFAGEQPFPMLPAVDHYAGSEKLMYKALQLQHELGPI
                     FDVTCDCEDGAAAGAAREHAEMVAAVIMSDANQHGRVGVRIHDLSHPHWQQDIEIIVT
                     RAGRRLAFVTLPKVRGSDDVRRLLGELARATTAAGLDRQIPAHVLIETHGALREVWDI
                     AALSAVESLDFGLMDFVSAHHGAIPGSAMQSPGQFEHPLVVRAKCEISAAALANGAVP
                     SHNVTTELKDLDVIRDNAQRARNEFGYLRMWSIHPNQIVPIFEAMRPDFSEVEDAAAI
                     LVAAQDCEWGPIQHHGKLHDRASYRYYWELLRRARATGMSLREDIKQRFFGSP"
     gene            5660..6082
                     /locus_tag="PLL14_01460"
     CDS             5660..6082
                     /locus_tag="PLL14_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOG02613.1"
                     /translation="MSLLALTAEVIEFREQRNWAQFHNLRNLIVSLNLEASELLELTQ
                     WKSDADMAALAEDARAREALHDECADVLIYLLLIAENAGIDLEAAARAKLARNAVRYP
                     VELCFGSSRKYAADTANPERSDGLTRCRAPVRTPDPQA"
     gene            complement(6057..6902)
                     /gene="rlmJ"
                     /locus_tag="PLL14_01465"
     CDS             complement(6057..6902)
                     /gene="rlmJ"
                     /locus_tag="PLL14_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HOG02614.1"
                     /translation="MLSYRHAFHAGNHADVLKHLVLVQLARYLCQKEQAFWYLDTHAG
                     AGSYALDSAAAAKLGEFRDGVGRLWGRKDLPAALADYLALVRKVNPDGGLKVYPGSPL
                     LALAIMRAQDRLRLFELHSREVLRLRENFQAFGKQVIVHDSDGFASLKALLPPPPRRA
                     VVLIDPPYEERRDYDRVVHSLKECLTRFPTGTYMLWYPQLTKLEAHDLPQRLQRLAAS
                     NWLQVTLCVRTPAGDGFGMHGSGLYILNPPWTLQSTLQEVMPYLVGVLGQDAGAGFTL
                     AGQVS"
     gene            complement(6950..8194)
                     /locus_tag="PLL14_01470"
     CDS             complement(6950..8194)
                     /locus_tag="PLL14_01470"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG02615.1"
                     /translation="MKSVDDFRLRFGNKELVPIMIGGMGVDISTAELALEAARLGGVG
                     HISDAMVNTVADRRFNAKFVKDKLRQYKFNVANSDKSMVRFDLGQLAEATEMHVGRTM
                     EAKRGDGLIFVNCMEKLTMNAPRETLRVRMAAALDAGIDGITLAAGLHLGSFALIEEH
                     PRFRDAKLGIIVSSLRALQLFLRKTARCNRLPDYVVVEGPLAGGHLGFGMDWAQYDLA
                     TIFGEIHRYLQAEHLDIPLIPAGGIFTGSDAVSFLDQGAAAVQVATRFTVAKECGLPE
                     DVKQRYFAASEDQIEVNQISPTGYPMRMLSNSPAIGDGIRPNCEAYGYLLDSSGNCSY
                     ITAYNREVAANPGVKKISVRDKTCLCTQMRNFDCWTCGHYAYRLKDTSKRLPDGSYQL
                     LSAEHIFLDYQFSTDGKLRVPE"
BASE COUNT         1773 a         2641 c         2358 g         1557 t
ORIGIN      
        1 gcgtcttcag cgcgatggat tcgacggaca gcggcttcat gatcaccggc ttgtaatcga
       61 gacgattgct ctcgcggtgc cacagggtgt gcttgtgcca gttgacgtcg tcgcggccgt
      121 tcgggttggc ggcggtatcc ggcgcgtcgt cgcgaacatg cgcaccgcgc gactccttgc
      181 gtgcttcggc cgaaatcatc gtcgccttgg cgacttcgat caggttgtcg agttccagcg
      241 cttcgacacg cgcggtgttc cacacctgcg atttgtcctt gatctcggtt ctgagcaccg
      301 ccttctcgac atcgagaatc ttcgccaccc cttcgaccag catgtccttg aagcgaaaaa
      361 cgccacaatg cttttgcatg gtccgctgca attcgagacg cgtttcattg acgctcgtcc
      421 caccggtctg cgtattgagg cgattcaggc gattcagcgt acgttcggcg taatccgctg
      481 gcaactcctt gagtccgatg gcccccgacc ggaggtcgtc gatcaccgaa tcgccagaag
      541 acttgccgaa aaccagcagg tccagcagcg aattggtccc cagccggttg gcaccatgca
      601 ccgaagcaca ggccacttcg ccggcggcat agaagccggg gatcggcgtc gcatcctgcg
      661 acaccacctg tcccttgtag ttggtcggca caccgcccat ctgatagtga caggtcggaa
      721 ccaccggaat cggcgccttg atcgggtcga tgccggcaaa ctggatcgaa atttcgcgaa
      781 tccccggcag ccgcttcatg atcgtcgccg gatcgaggtg ggtaatgtcg agcaggacaa
      841 aatccttgtt cgggccacaa ccacgtccct cgttgatctc cgtgaccatc gcccgcgaca
      901 ccacgtcgcg cgacgcaagg tccttggcgt tgggagcgta gcgctccatg aaacgttcgt
      961 tggctgagtt gcgcagaata ccgccctcac cgcgcacacc ttccgtaatc agcacgccgg
     1021 caccggcgac accggtggga tggaactgcc agaattccat atcttcgagt gcgatgccgg
     1081 cgcgcgccgc catacccaga ccgtcaccgg tattgatgaa ggcattggtc gacgaataga
     1141 agatccggcc ggctccaccg gtggcgaaga ccgttgcctt ggcatggaag gcgacgacat
     1201 caccagtctc catttcgagt gcgatgaccc cgagtacctg ccccttgtcg tcgcggatca
     1261 gatcaagggc catccattcg acgaagaact gggtattggc acgtacgttc cgttgataca
     1321 gcgcatgcag catcgcgtgg ccagtacggt cggcggcggc gcaagcacgc cgtaccggct
     1381 tttcgccaaa attcgacatg tgcccgccga aaggacgctg gtagatcttg ccttcgtccg
     1441 tacggtcgaa aggcatgcca aaatgctcaa gttcgacgac cacctcgggc gccatgcggc
     1501 acatgtattc gatcgcgtcc tggtcaccga gccagtcaga acctttcacc gtatcgtaca
     1561 tatgccagtg ccagtggtcc tcctcggagt tgccgagcga ggccgagacg ccgccctgcg
     1621 cagccacggt atgcgaacgg gtgggaaaaa ccttggacag gacggccgtc ttcaatccgg
     1681 cttcggaaag ctggatcgcc gcccgcagac ccgaaccgcc agccccaaca atgactgcat
     1741 cgaacttacg tactggaatg gttagcttgc tcacttacat tctccacaga atctgaaccg
     1801 cccagccggc atatccgacc agcaccgcag ccgtcgcaat catcatcaac agacgcaagc
     1861 catccggctt gacgtaatcc atccagatat cccgaatccc aatccaggca tggtagaaga
     1921 cgctgacaaa gaacaggaat gttgcaaatt tcatgaagcc gttggcaaaa atgccgcgcc
     1981 aggcctcaaa ggtatcgggc ccgacagcac cgacgacgac taggaagatg agcgtataga
     2041 cggccatcac gatggcggtg gcgcgctgga tgatccagtc cttgaggccg taatgcgccc
     2101 cgacgagaat acgatttacc atagcactct agctccgatg atcagggtca gggcaatgct
     2161 gacgacgaaa acgatcttgc tcgaaaggcg cgccgcctcg agttccaccc ccttgtgcag
     2221 atccagcagc aggtagcgga tgccggcaca gaagtggtgc atgtagaacc agaccaggcc
     2281 gagcaggaca atcttcgcca acgggtgacc gaccaccgct ttgacgccgg caaaggattc
     2341 aggagacgtg aggctaccct gaaacagcca tagcaggaac ggcagcagca ggaacatcgc
     2401 cgctccgctg atgcggtgaa tgattgacag gatccccgga aggggtagcc tgatagtggg
     2461 caaatccaaa ttctttggac gctttttctt gattgccagc tctgccatgc acgtcatccc
     2521 tcatgtgtta tacggccgaa tgcacgtcat ccggcccctg cgataccacg ctagattata
     2581 aaccatccaa gcgtgcctga attgcccgac gggcctgttc cagcactcac cccagttcat
     2641 tgtgataatg ataattgcgt gtcgaataga atccccggcg ccattcgacg ggtttgtgac
     2701 cataggtgaa ggtgactctt tcgaccagca gcaggggact tccatcggcc acctgcagcc
     2761 actctgcgct gactctgtca gccgagacgg cgcgtagccg ttcatcggcg cgaatcatgc
     2821 gcacgccata gcgagtctcg aacaggctgt acagcgactc tcccgaacgc agcacatcga
     2881 gcgacaagtc gggaaagagt tcgctgggca aatagatctc gtcgaaaacg accggctcgt
     2941 cgcccaactt cagcaaccgg cgaacaatcg taatcggcgc cccggtgtca agagcgagag
     3001 tacgtgcgac gtctgcgccg gccttggcgc gccagcattc gagcggcacg ctttgggaaa
     3061 cctgcagatc cccatcattc ggcaccagtc ggagaaagcg gaaaaaggaa cccggatcct
     3121 tgtgagtggc gacgaaggtt cccttgccct gctgccgtac caggaggttc tcggcagcca
     3181 tctcgtcaat cgccttgcga accgtaccct ggctcacacc gaagcggcca gcaagttccg
     3241 actcgctcgg gatcgggtca cccgggcccc actcgccgga ctccagatca cgcaggatca
     3301 attccttgat ctgccgatac agggggctga atgtgggcga gtgcactgac gaaggccggc
     3361 tcatggatcg tcatttcagc ataatttcac acttccgtcc atcccgctgg agttatttat
     3421 atcttatata agacataaga tatatttgac actccgcgac cttagatctt atgatttgag
     3481 tgatttgctg ctcctgcact caacggcgtg cggggtcaac gccaatcgta ttctgcccat
     3541 ttttgctcat caccaataac aggagccgca tcatgtccaa agcccccatg cgcgtcgcgg
     3601 tcaccggcgc cgccggtcaa atcggttaca gtctgctctt ccggattgcc tccggcgaaa
     3661 tgctcggcaa ggatcaaccg gtcatactcc aactccttga tctgccgcaa gcacaaaaag
     3721 cctgtcaggg cgtgatcatg gaactcgagg actgtgcctt cccgctactc gccggcatgt
     3781 tcgccaccga cgacccgaac ctcgccttca aggacgctga cgtctgcctg ctggtcggcg
     3841 cccgtccgcg cggcccgggc atggagcggg cagacctgct gaccgccaac ggcgccatct
     3901 tcaccgttca gggcaaagcc attgcggaga acgccaagga ggatgtccgc gtgctcgtcg
     3961 tcggcaaccc ctgcaacacc aatgccctga tcgccggcgc cgccgctcga aaagtcggcc
     4021 gcaccaatcc aggcaactac catggcatgc tgcgcctcga tcacaaccgt gccttgtcac
     4081 aacttgctgc gaagaccgga cggccggtag ccagcttcaa gcaactcgtc gtgtggggca
     4141 atcattcgcc gacgatgtac gccgactacc gcaattgcac ttctgatggc gataacgtca
     4201 agatgctggt caatgatcca gtgtggaaca acgatgtgtt cctgccgacc gtcggcaagc
     4261 gcggcgcggc gatcatcgac gcgcgcggcc tgtcgtccgc cgcttcggca gccaacgcag
     4321 ccattgatca catgcacgac tgggtcctcg gttccgacga gtgggtgacc atgggcgtcc
     4381 cctcggacgg ctcctacggc atcccgaccg ggattgtttt cggcttcccg tgcgaatgca
     4441 agggcggttc gtatactatt atccagggca tcgaaatcga cgattactca cgcgagaaga
     4501 tcaacaagac gctcaaggaa ctcaccgacg aagcagaagc cgtgcagggc atgctttaaa
     4561 caacggcgca accagtcaaa agccgcggcc agaccgcggc ttttttgcct ccgcattccc
     4621 tcccagggca cagtagaata cgcaatccca agttcgaata cacagacacc ccgatgcgcc
     4681 atcccagcca ggtcctgttc gcaggagagc agccgtttcc catgttgccc gctgtagacc
     4741 actacgccgg ctcggaaaaa ttgatgtaca aggcactcca gttgcagcac gaattggggc
     4801 cgattttcga cgtcacctgc gactgcgagg atggcgccgc agccggtgct gcccgggaac
     4861 acgccgaaat ggtggccgcg gtcatcatgt ccgacgccaa ccagcacggg cgcgttggcg
     4921 tccgcattca cgacctcagc catccgcact ggcaacagga catcgaaatt atcgtcaccc
     4981 gtgccgggcg acgcctggcg ttcgtcaccc tgccgaaagt gcgcgggagc gacgatgtcc
     5041 gaaggctgct gggcgagttg gcgcgcgcaa ccaccgcagc agggctcgac cgccagattc
     5101 ccgcacacgt cctgatcgag acgcatggtg ccctgcgtga agtctgggat atcgcagcgt
     5161 tgtctgcagt ggaatcgctc gatttcggac ttatggattt tgtcagcgca caccacggcg
     5221 ccatcccggg ctcagcgatg cagagcccag gtcagttcga gcatccgctg gtcgtccgcg
     5281 ccaagtgtga aatctcagcc gcagcactgg ccaatggcgc agtaccgtca cacaacgtga
     5341 ccaccgaact caaggatctc gatgtcatcc gcgacaacgc gcagcgcgcg cgcaacgagt
     5401 tcggctacct gcgcatgtgg agtattcacc ccaatcagat cgtgcccatc ttcgaagcca
     5461 tgcgccccga cttctcggaa gtcgaagatg cagccgcaat cctagtcgcc gcccaggatt
     5521 gcgaatgggg gccgatccag caccatggca aactgcatga ccgcgcctcg taccgctact
     5581 actgggaact gctcagacgc gcacgcgcaa ccggcatgag cctgcgcgag gacatcaaac
     5641 aacgtttctt cggcagccca tgagtctgct ggcgttgacg gcggaagtca tcgaatttcg
     5701 cgagcaacgg aactgggcgc agttccacaa cttgcgcaac ctgatcgtct cgctgaatct
     5761 cgaggccagt gaactgctcg aattgacgca gtggaaaagc gatgccgaca tggccgccct
     5821 ggccgaggat gcccgggctc gggaagcact ccacgacgag tgcgccgatg tcctcattta
     5881 tctgctgctg atcgccgaaa atgccggcat cgaccttgaa gccgcagcac gtgccaaact
     5941 cgccaggaat gccgtcagat acccggtgga actctgtttc ggttcgagcc gcaagtacgc
     6001 agcggacacc gccaatccag agcggtccga cggcttgact cgctgccggg cgcccgtcag
     6061 gacacctgac ccgcaagcgt aaaaccggcg cctgcatcct gcccgagcac cccgaccaga
     6121 tagggcatca cctcctgcaa ggtggactgc agggtccacg gcggattgag gatgtacagg
     6181 ccgctgccgt gcattccgaa accgtcaccg gccggggtgc ggacgcatag cgtcacttgc
     6241 agccagttgc tggccgccag gcgttgcaga cgttgcggca agtcatgcgc ttcgagcttg
     6301 gtgagttgcg gataccacag catgtaggtg ccagtcggga agcgtgtcag gcactccttg
     6361 agcgaatgca ccacccggtc gtagtcgcgg cgctcttcat acggcgggtc gatcagtacc
     6421 acggcacgcc tcggcggtgg cggtaacagg gccttgagcg acgcaaatcc atcgctgtcg
     6481 tgaacgatca cctgcttgcc gaaggcctgg aaattctccc gcagccgcag cacctcgcgg
     6541 ctatgcagtt cgaaaaggcg caggcggtcc tgtgcgcgca tgattgcgag cgccagcaag
     6601 ggtgaaccgg gatagacctt caaaccgccg tcgggattga ccttgcgcac cagcgccaag
     6661 taatcagcca gagccgccgg cagatccttg cgcccccaca gccgaccgac accatcccga
     6721 aactccccca gtttggctgc cgcagcggaa tccagagcat aggatcccgc gccggcgtgc
     6781 gtatcgaggt accagaaggc ttgctccttc tggcagaggt agcgcgcaag ctgcaccagc
     6841 accagatgct tgagcacatc ggcgtggttc ccggcatgga aggcatgacg ataactgagc
     6901 atccgctaac gcccgtccgc agtggcggag gaacgccgca gccagcgcac tactccggca
     6961 cccggagctt gccgtcggtg ctgaactgat agtcgaggaa gatgtgctcg gcgctcagca
     7021 actggtagct gccgtcgggc aagcgcttcg acgtatcctt caggcggtac gcatagtgac
     7081 cacaggtcca gcaatcgaag ttgcgcattt gcgtacatag acaggtcttg tcccgaaccg
     7141 atatcttctt gacgcccgga ttggccgcca cctcgcggtt atacgcggta atgtaggaac
     7201 agttgccgct gctgtcgaga agatacccat aggcctcgca attgggccga atcccgtcgc
     7261 caatcgccgg gctgttgctc aacatgcgca tcgggtaacc ggtaggcgag atctggttga
     7321 cctcgatctg gtcttcactg gcagcgaaat agcgttgttt gacgtcttcc ggcaaaccac
     7381 attccttggc cacggtgaaa cgcgtagcca cctgaactgc cgcagccccc tggtcgagga
     7441 aggaaacggc gtcactcccg gtgaagatac cgccggccgg aatcagcgga atatccagat
     7501 gctcggcctg cagataacgg tgaatttcac cgaagatggt cgccaggtca tactgggccc
     7561 agtccatgcc gaaacccaga tgcccgccgg ccagtggccc ttcaaccacc acatagtccg
     7621 gtagccggtt gcagcgtgcg gtcttgcgca ggaacaattg cagggcgcgc agcgaggaga
     7681 cgatgattcc cagcttggcg tcgcggaaac gcggatgctc ctcgatcagg gcaaacgacc
     7741 ccaggtgcag accggcagcc agagtgatgc cgtcgatgcc ggcatcgagc gccgccgcca
     7801 tgcgcacgcg caaagtctcg cgaggcgcgt tcatggtcag cttctccatg cagttcacga
     7861 agatcaggcc gtcaccacgt ttcgcttcca tggttcggcc gacgtgcatc tcggtggctt
     7921 cggcgagttg tccgagatcg aagcgcacca tcgacttgtc ggagttggcg acgttgaact
     7981 tgtactgcct gagcttgtcc ttgacgaact ttgcattgaa ccgccgatcg gccacggtat
     8041 tcaccatggc atcggaaata tgtccgacac cgccaagccg agccgcttca agcgcgagtt
     8101 cggcggttga aatatccacc cccatgccgc cgatcatgat cggcaccagt tccttgttgc
     8161 cgaaccgcag acggaaatca tcaacgctct tcatccctac ccttcccaac ccgcgtcagc
     8221 gttcccaact cgcctatagg tgatcaccac gacccccaac cccggcatta tcgctcatca
     8281 aatgcctgta tggcttgatg catgcaagga aatcgcgcgc acgggcggc
//
LOCUS       DAOSIT010000029         8167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_8190_length_8167_cov_8.599112, whole genome shotgun sequence.
ACCESSION   DAOSIT010000029 DAOSIT010000000
VERSION     DAOSIT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8190_length_8167_cov_8.599112"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1293
                     /locus_tag="PLL14_01475"
     CDS             427..1293
                     /locus_tag="PLL14_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02616.1"
                     /translation="MTAHQSACSKHFAAAALGVLCLTAAAPACAEAGSPQVNRFDGQW
                     HFSLTPYLWLPTIYTSASFAGPLGIAPGVDANMHTTPGTYLHHLSFAFFLGGEARKGE
                     WSLFTDYMYLKFKDQNASVRTVTGPAGLVTRVVDRGSTMDLKSNVWTLAGGYTAWRRG
                     NSHLDVLVGTRYLSMDSTLDWNVSGAAGLLPGRQRSLSSNVDKWDAIVGLKGQLTLSD
                     DGKWFMPYYLDVGTGSRNTTWQALLGAGYRYGWGDVTLVLRNLSYDFSGHHDDNIDAR
                     FTGLGLGATFVF"
     gene            1381..3840
                     /locus_tag="PLL14_01480"
     CDS             1381..3840
                     /locus_tag="PLL14_01480"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOG02617.1"
                     /translation="MACGGTSLAQPAASAPGSPSATTSISGEQLPPPPPKFGGRIERN
                     AAQSKPYWPARVEPPKGAPNVLLIITDDTGYGTPSTFGGVVPTPALDRVAANGLRYTN
                     FNSTALCSPTRAALLTGRNHHSAGFGVISEQSSGYPGYNSIIERDKATIGRILKDNGY
                     RTSWFGKDHNVPAFQASQDGPFDQWPTGMGFEYFYGFIGGDANQWQPNLFRNTTQIYP
                     FIGKPGWNLTTAMADDAISYMNRINALAPDQPFFIKYAPGGVHAPHHPTPEWIEKISK
                     LKLFDKGWNALREQIFENQKKLGVIPANARLTPWPKDLLKEWDTLSADEKKMFIRQVD
                     VFAAYVAYTDHEIGRVIQAVEDLGKLDNTLVIYISGDNGGSAEGTLVGTPNEVAMFNG
                     LVIPVEEQLKYFYDVWGSDKTYNHMAVPWSWAFDTPFSWTKQVASHFGGVRQGMAIMW
                     PKVITDKGGMRHQFHHVIDIVPTILEAAKIRQPKVVDGIRQSPIEGVSMLYTFDKANE
                     NAPSTHKTQYFEMMGDHAIYHDGWIASSKVMRPPWVTMSPTSQDPAGYPWELYELDKD
                     WTQSEDVAAKYPQKVKEMEKIFWAEAKKYQVLPLDSSVATRLVTPRPSITAGRNVFTW
                     TAPLTGTPNGDAPSILNASYNFKAEVEIPQGLAQGMLITQGGRFGGYGFYVLKGKPVF
                     TWNLVDMKRVKWEGSEALTPGKHTLEFDFKYDGLGMGTLAFNNLSGIGRGGTGVLKVD
                     GNVVAEQKMERTLPLILQWDEALDIGSDTLTGVNDQDYLPPFAFNGKLNRITLSIDRP
                     KLTPADEKKLLAAQRNNKASE"
     gene            3902..4186
                     /locus_tag="PLL14_01485"
     CDS             3902..4186
                     /locus_tag="PLL14_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02618.1"
                     /translation="MSRNLWRFAAVPAVFFAGVSLAQFPVLDMVANKVIEKYQNSSCE
                     QLWAQKGQPKSPREQEMIQMLRNDPAMRSEFINRVAAPMANKMFECGMIP"
     gene            4236..5114
                     /locus_tag="PLL14_01490"
     CDS             4236..5114
                     /locus_tag="PLL14_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HOG02619.1"
                     /translation="MIQNKSARRAALLALSLLLAGSVGAQQALAATKAAKPARTSSRV
                     VAPAPRPVLESRAIDLLKGMSARLAAARSMAFTATVMYEAPSLLGPALAYTTSSEVLM
                     QRPDKLRVLTRGDGPASEFYYDGKTMTAFAPVENLVAVAAAPATVDATLKAAFESAAI
                     YFPFSDVLLADPYKNIGEGLTNAFYIGQSKVVGGTVTNMLVFVNDYVFVQIWIGAEDQ
                     LPRMLRAVFRDDPLRLRHQLELSNWQLDPAVAADAFSPSKTAGAAAIAFAHPAAPGFT
                     PPARGKPGTAPTSKNQ"
     gene            5125..5808
                     /locus_tag="PLL14_01495"
     CDS             5125..5808
                     /locus_tag="PLL14_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02620.1"
                     /translation="MNRIVIALAGVISAMLVTGEAAAWGSANRFGGSTEHVAGVGTEH
                     TNAWGGSSEHAYGGGSEHTNMYGGSTAGRYGEGAEHTNVYGGTTAARYGEGAVHTTSY
                     GATAYRPPVPPPVYPYHPPVAVPYYAAAGCAGCAAAAGAVVGVAAGAAVASANTAAAT
                     SSAYSSGYAAGSTNTAAAYAMGVNYAALPAGAMAVQKYGNTYYLVGNTWFQPAYGANG
                     VYYRVVPTP"
     gene            complement(5936..>6502)
                     /locus_tag="PLL14_01500"
     CDS             complement(5936..>6502)
                     /locus_tag="PLL14_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG02621.1"
                     /translation="LAVAGSAVLVVLAARDGDPWKVVSVSIYGATLVLLYCFSTLYHS
                     FRGRAKDILRKLDHQSIYLLIAGSYTPFCLVTLRGPWGWSLLAVVWGLAVVGGLQELW
                     LRSEARVLSVVIYVVMGWAVVVALVPLLQALGTAGFAWLAVGGVLYTTGIIFYALDTR
                     LRHAHGVWHLFVLGGSAAHYLVILIYVL"
     assembly_gap    6503..6602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6609..>8167
                     /locus_tag="PLL14_01505"
     CDS             6609..>8167
                     /locus_tag="PLL14_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOG02622.1"
                     /translation="MRNNQPVTAVETVIPEGEFIYSRTNLQGTIEEANEAFANISAYA
                     RAEMIGQPHNMVRHPDMPEAAFADLWADLKAGRPWRGLVKNRRKDGGYYWVVANASPV
                     RENGQIVGYQSVRARPTRPEIAAAEAAYRRIKEGDQSLRVEHGRVVRVLPRVVDWFGS
                     LHTQMLLMGLLAVVPGMVGLLSSLLEGRLLNHVHDVLAVIAAVYALYFLFWVTPHVSR
                     DLKATADHLETVLSTGDLKTRLDINRRDIIGRVARLADNFIASVQATVQGMADTAKQV
                     DDANNHVRAGVHNVNQSAVVQSDATSASAAAVEQVTVSIGEVAAHAASTEAAAHKAGD
                     VAQAGEELSLRASNTILSLAGTVKASAQQVESLGQRSEEISRIVAVIKEVADQTNLLA
                     LNAAIEAARAGEAGRGFSVVADEVRKLAERTGKATQEISTMISSIQAETKQAVEGMRN
                     GAHEVEESVRLVQEAREALRGINEQMRTTVSMVSEITHSSNEQQSAMTELAQNVERVA
                     SMTEQNVSVTR"
BASE COUNT         1589 a         2697 c         2531 g         1250 t
ORIGIN      
        1 cggggccgtt gatcatgtcg cctcctccaa tccgttgatg aacgtgatgc gccgcaggca
       61 gatgacccgc cgcgggcaca tctttccaca ttgcccgtgc gatccgcaat gggcaggtct
      121 ggcctggcca tcgcggatcg cctgatggtc cttcccgccg gccgctgctg gcgatggctg
      181 gggaacggcg gtggctgcga cgacaagcgc agatacctgc cccacatatc ctggggctgt
      241 ggaataattc gcagcgacgc aatgaacttg cgttctcttc tcgggagggg aagccatgta
      301 caaacgctgt gcaatagcgg tcatcgccgc gatcgggtgc gtgtcggcgc caggcatcca
      361 cgccgccgac tggtcgtggg atccgataac caacaggatg catacgccag ggaggaaaaa
      421 ttgaccatga ccgcacacca atccgcttgc agcaaacact ttgccgccgc cgcgctgggc
      481 gtgctttgcc tgacggctgc ggcaccggcc tgcgccgagg cgggcagtcc gcaggtgaac
      541 aggttcgacg gacagtggca cttcagcctt acaccttacc tctggctgcc gaccatctat
      601 acctcggcgt cgttcgccgg accactgggc atcgctccgg gcgtcgatgc caacatgcac
      661 accactcccg gcacctacct ccatcatctc tcgttcgcct tcttccttgg cggcgaggcg
      721 cgcaaaggcg agtggtcact gttcaccgac tatatgtacc tcaagttcaa ggaccagaac
      781 gccagcgtca ggaccgtcac cggaccagcc ggactggtca cgcgcgtcgt cgatcggggc
      841 agcacgatgg atctgaaatc gaacgtgtgg accctcgccg gcggctacac cgcctggcgc
      901 cggggcaact cgcacctcga cgttctggtg ggcacccgtt acctgtcgat ggactccacc
      961 ctcgactgga acgtcagcgg cgccgccggg ctattgccgg ggcggcagcg cagtctttcg
     1021 agcaacgtgg acaaatggga cgccatcgtc ggcctgaaag gacagctcac tctcagcgac
     1081 gacggcaagt ggttcatgcc ctactacctc gacgtcggca ccggttcgcg gaacaccacc
     1141 tggcaggcct tgctcggtgc cggctaccgc tacggctggg gtgacgtgac gctggtgctg
     1201 cgcaatctct cgtacgactt cagcggccac cacgacgaca acatcgacgc gcgcttcacc
     1261 ggcctcggac tcggcgcgac cttcgtgttc taaagctcta ttgatctgca gcaccaccag
     1321 gaggtagcta tgcaaacaac acgacgtcga tgggcttttc tcgctttgct ggccctgtcc
     1381 atggcctgcg gtggcaccag cctcgcccag ccagccgcca gcgcgccggg ttcgccgagc
     1441 gccacgacca gcatcagtgg cgagcagttg ccgccaccgc caccgaagtt cggtggcagg
     1501 atcgaacgta atgccgcaca atccaaaccc tactggccgg cacgcgtcga gccacccaag
     1561 ggcgcgccga acgtgctgct gatcatcacc gacgataccg gctacggcac gccgagcacc
     1621 tttggcggcg tcgtgccgac accggcgctc gaccgcgtgg cggcgaatgg cctgcgttac
     1681 accaacttca actcgacggc cctgtgttcg ccgacgcgcg ccgcactgct caccggccgc
     1741 aaccatcact cggccggctt cggcgtgatc tccgaacagt ccagcggcta tcccggctac
     1801 aacagcatca tcgagcgcga caaggcgacg atcggccgca ttctgaagga caacggctac
     1861 cgcacctcgt ggttcggcaa ggaccacaac gtgccggcgt tccaggccag ccaggatggc
     1921 ccgttcgatc agtggccaac cggcatgggc ttcgagtact tctacggctt catcggcggc
     1981 gacgccaacc agtggcagcc gaacctgttc cgcaacacca cgcagatcta ccccttcatc
     2041 ggcaagccgg gatggaatct caccaccgcc atggccgacg acgcgatctc gtacatgaac
     2101 cggatcaacg cacttgcgcc ggatcagccg ttcttcatca agtacgcgcc gggcggcgtg
     2161 catgcgccgc atcacccgac gcccgagtgg atcgagaaga tcagcaagct gaagctcttc
     2221 gacaagggct ggaatgcgct gcgtgagcag atcttcgaaa accagaagaa actcggcgtg
     2281 attccggcca acgccaggct gaccccctgg ccgaaggacc tgctcaagga gtgggacacg
     2341 ctgagcgctg acgagaagaa gatgttcatc cgtcaggtgg atgtcttcgc cgcctacgtg
     2401 gcgtataccg accacgagat cggccgcgtc atccaggccg tcgaggatct gggcaagctc
     2461 gacaacaccc tggtgatcta catcagcggc gacaacggcg gcagcgccga ggggacgctg
     2521 gtcggcacgc ccaacgaagt ggcgatgttc aacggtctcg tgattccggt cgaggagcag
     2581 ctcaagtact tctacgacgt ctggggctcc gacaagacct acaaccacat ggccgtgccc
     2641 tggtcatggg ccttcgacac gccgttctcc tggaccaagc aggtcgcctc gcacttcggc
     2701 ggcgtgcgcc agggcatggc gatcatgtgg ccgaaggtga tcaccgacaa aggcggcatg
     2761 cgtcaccagt tccaccacgt gatcgacatc gtgccgacca tcctcgaagc ggcgaagatc
     2821 aggcagccga aagtggtcga tggcatcagg cagagcccga tcgaaggcgt gagcatgctg
     2881 tacaccttcg acaaggccaa cgaaaacgcg ccatcgacgc acaagacgca gtacttcgag
     2941 atgatgggcg accatgccat ctaccacgac ggctggatcg ccagcagcaa ggtgatgcgg
     3001 ccgccgtggg tcacgatgtc gccgaccagc caggatccgg ccggctaccc gtgggaactg
     3061 tacgagctcg acaaggactg gacgcagtcc gaggatgtcg ccgcgaagta cccgcagaag
     3121 gtgaaggaga tggagaagat cttctgggcc gaggcgaaga agtatcaggt cttgccgctc
     3181 gactcgtcgg tggccacccg cctggtgacg ccgcgcccga gcatcactgc cggtcgcaac
     3241 gtgttcacct ggacggctcc gctcaccggt acgccaaacg gcgacgcccc gagcatcctc
     3301 aacgcctcgt acaacttcaa ggcggaagtc gagattccgc agggtctggc gcagggcatg
     3361 ctgatcaccc agggtggccg cttcggtggc tacggcttct atgtgctcaa gggaaagccg
     3421 gtgttcacct ggaacctggt cgacatgaag cgcgtgaaat gggagggtag cgaagccctg
     3481 acgcccggca agcacaccct ggagttcgac ttcaagtacg atggcctggg catgggcacg
     3541 ctggccttca acaacctctc cggcatcggc cgtggcggta ccggcgtgct caaggtggac
     3601 ggcaatgtgg tcgccgagca gaagatggag cgtacgctgc cgctgatctt gcagtgggac
     3661 gaggcccttg acatcggctc ggacaccctg accggagtga acgaccagga ctacctgccg
     3721 ccgttcgctt tcaacggcaa gctcaacagg attacactga gcattgaccg gccaaagctg
     3781 acgcctgccg acgagaagaa gctgctggcc gcgcagcgca acaacaaggc cagcgagtag
     3841 catcaccaga tcgggcagcc cctgcgcggg ctgccctcgc aacccagtgg aggagtcaag
     3901 catgagcagg aatctctggc ggtttgccgc cgtgccggca gtgtttttcg ccggcgtcag
     3961 tctggcgcag ttcccggttc tcgacatggt cgccaacaag gtgatcgaga agtatcagaa
     4021 ctcgagttgt gagcaactgt gggcacagaa ggggcagccg aagtcgccgc gtgaacagga
     4081 aatgatccag atgctgcgca acgaccccgc gatgcgtagc gagttcatca accgggtggc
     4141 tgcaccgatg gccaacaaga tgttcgaatg cgggatgatt ccgtaagcgg ctcaccggcg
     4201 gcacttcgct tcgttcaacc atgaggagaa acttgatgat tcagaacaaa tccgccaggc
     4261 gcgcagcgct gctcgccctg agcctgctgc tggccgggag cgtcggcgca cagcaggccc
     4321 tggccgcgac gaaagccgcg aagccggcca ggacgtcgag ccgggtcgtc gcgccggccc
     4381 ccagaccggt gctcgaaagt cgcgccatcg acctcctgaa aggcatgagc gcgcgcctgg
     4441 cggcggcacg ctcgatggcg ttcaccgcaa ccgtgatgta cgaagcgccg agcctgctgg
     4501 gccctgccct cgcctacacg acaagctccg aggttctgat gcagcggccg gacaagctgc
     4561 gcgtgctgac ccgcggcgac ggcccggcgt ccgagttcta ctacgacgga aagacgatga
     4621 ccgcctttgc gccggtggaa aatctggtcg ccgtcgccgc cgccccggcg acggtcgacg
     4681 ccacgctcaa ggcggccttc gagagtgcgg cgatctactt tccgttctcc gacgtgctcc
     4741 tggccgaccc ctacaagaac atcggcgagg gcctgaccaa cgccttctac atcgggcagt
     4801 cgaaagtggt cggcggcacc gtgaccaaca tgctggtgtt cgtcaacgac tacgtcttcg
     4861 tgcagatctg gattggcgca gaagaccagt taccgcgcat gctgcgggcg gtgtttcgcg
     4921 atgatccgct acggctgcgt caccagttgg agctttccaa ctggcaactc gacccggcgg
     4981 tggctgccga tgccttctcg ccgagcaaga ctgccggcgc ggcggcgatc gctttcgccc
     5041 atccggctgc gcccggcttc acgccgccgg cacgcggcaa gccgggcacc gcgccaacca
     5101 gcaagaacca gtgaggagtc acagatgaac aggatcgtaa ttgcccttgc gggcgtgatc
     5161 agcgccatgc tggtgaccgg tgaggccgcc gcctggggca gcgccaaccg ttttggcggc
     5221 agtaccgagc acgtcgccgg agtaggtacc gagcacacca acgcctgggg cggcagctcg
     5281 gaacacgcct acggcggcgg cagcgagcac accaacatgt atggcggcag caccgccggc
     5341 cggtacggtg agggggccga gcataccaac gtctacggcg gcaccaccgc cgccaggtac
     5401 ggcgaggggg cggtgcacac caccagctac ggcgccaccg cctaccgtcc accggttcct
     5461 cctcccgttt acccgtacca cccgccggtg gcggtgccct actacgcggc agcgggctgc
     5521 gccggctgcg ccgccgcggc cggggcggtc gtcggcgtcg ccgccggcgc cgccgtcgct
     5581 tcggcgaaca ccgccgccgc gaccagcagc gcctactcct cgggttatgc cgcgggcagt
     5641 accaacaccg ctgcggccta tgcgatgggg gtcaactacg ccgccctgcc ggcaggcgcg
     5701 atggccgttc agaagtacgg caacacctac tatctggtcg ggaacacctg gttccagccg
     5761 gcctacggcg ccaatggcgt ctattaccgg gtggtcccga cgccgtagga tgcggcgccg
     5821 gggtcaggac cgactccgac agggcgcgcg acgtggcggc agtgaacgcg ccgccacgcc
     5881 gcggttcgcg tgcccggcag cagtcgccat ggccgctgct ctagccggat gagcttcaaa
     5941 gcacgtagat caggatcacc agatagtgcg cggcgctgcc ccccaggacg aacaggtgcc
     6001 aaacaccgtg cgcgtgtcgc aggcgcgtgt cgagagcata gaagatgatc cccgtggtgt
     6061 acagcacgcc gcccaccgca agccaggcga aacccgccgt ccccagggcc tgcagcaagg
     6121 gaaccagcgc caccaccacc gcccagccca tgaccacgta gatcaccacc gacagaaccc
     6181 gcgcctcgct gcgcagccac agttcctgca gaccgccgac cacggcaagc ccccacacca
     6241 ccgcgagcag cgaccagccc cagggtccgc gcagggtgac cagacagaac ggcgtataac
     6301 tgccggcgat cagcaggtag atactctggt ggtccagctt gcgcaggatg tccttggcac
     6361 gcccgcgaaa gctgtggtaa agcgtggaaa agcagtacag aaggaccagc gtggccccgt
     6421 agatcgagac gctcaccacc ttccaggggt cgccgtcgcg cgccgcgagc accaccagca
     6481 cggccgagcc ggccactgcc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nncctaccat gcgaaacaac cagcccgtaa ccgcagtcga gacggtcatt cccgaaggcg
     6661 agttcatcta ctcgcggacc aacctccagg gtaccatcga ggaagccaac gaggctttcg
     6721 cgaacatcag cgcctacgcc cgtgccgaga tgatcggaca accgcacaat atggtccgcc
     6781 acccggacat gccggaagcg gccttcgccg atctgtgggc cgacctcaag gccggtcgcc
     6841 cctggcgcgg cctggtcaag aaccgccgca aggacggtgg ctattactgg gtcgtcgcca
     6901 acgcttcgcc ggtacgcgaa aacggccaga tcgtcggcta ccagtcggtg cgggcgcgcc
     6961 ccacccggcc ggaaatcgcg gccgccgaag cggcctatcg acggatcaag gagggtgacc
     7021 aatccttgcg cgtcgagcat ggccgggtgg tccgcgtgct gcctcgtgtc gtggactggt
     7081 tcggcagtct gcacacccaa atgctcctca tgggcttgct ggccgttgtc cccggtatgg
     7141 tcggtctgct gtccagcctg ctcgaaggca ggctgctgaa tcatgttcac gacgtcctcg
     7201 cggtgatcgc cgcggtgtat gccctgtact tcctgttctg ggtgacgccc cacgtatcac
     7261 gcgatctcaa ggccactgcc gaccacctcg aaaccgtcct ttccaccggt gatctgaaga
     7321 cgcgcctgga catcaaccgg cgcgacatca tcggacgcgt cgcccgcctg gccgacaatt
     7381 tcatcgcctc ggtacaggcc accgtacaag gcatggcgga taccgccaag caggtggacg
     7441 acgccaacaa ccatgtgcgt gccggcgtgc acaacgtcaa ccagtcggcg gtcgtgcaaa
     7501 gcgacgccac ctcggcgtcg gcagcggctg tcgaacaggt cacggtatcg atcggcgaag
     7561 tggcggcgca tgccgccagt accgaagccg cggctcacaa ggcgggtgac gtggcacagg
     7621 ccggcgaaga gctttcgctg cgggcgagca ataccatcct ctcgctggcc ggaacggtga
     7681 aggcctcggc acagcaggtc gaatcgctgg ggcagcgctc cgaggagatc agccgcatcg
     7741 tggcggtgat caaggaggtc gcggaccaga ccaacctgct ggccctcaac gccgccatcg
     7801 aagccgcacg cgcgggtgag gccggacgag ggttttccgt ggtcgccgat gaggtgcgca
     7861 aactcgcgga gcgtaccggc aaggcaacgc aggaaatcag caccatgatc agcagcatcc
     7921 aggccgaaac caagcaggcg gtcgagggca tgcgcaatgg cgcgcacgag gtcgaggaga
     7981 gcgttcgtct ggtgcaggaa gcccgcgaag cgctgcgcgg catcaacgag caaatgcgca
     8041 cgacggtgag catggtcagt gaaatcacgc actcctccaa tgagcagcaa tcggcaatga
     8101 ccgaactggc ccagaacgtc gagcgcgtcg cctcgatgac cgaacagaac gtgtcggtga
     8161 cccgcga
//
LOCUS       DAOSIT010000030         8036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_8346_length_8036_cov_10.701917, whole genome shotgun sequence.
ACCESSION   DAOSIT010000030 DAOSIT010000000
VERSION     DAOSIT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_8036_cov_10.701917"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLL14_01510"
     CDS             <1..334
                     /locus_tag="PLL14_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOG02623.1"
                     /translation="FEQADFALVYFAVGGFFATFVFAISVIALPLMLDRKTDAITAAI
                     ASLVACGRNPGTMLLWAACIVVLVSVGFATLFFGLIVTMPLVGHATWHAYRDLVDNDD
                     SPEPGNRR"
     gene            complement(366..1226)
                     /locus_tag="PLL14_01515"
     CDS             complement(366..1226)
                     /locus_tag="PLL14_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG02624.1"
                     /translation="MRLRRWILLASTTVGLCLAVPVHAAGTLDKIKASQAIALGYRET
                     SIPFSYTGDDNQPWGYSVDLCTRVAAAIVKQLGLEELQLQWVAVTPENRIAKLKSGEI
                     DLECGSTTSSLTRMQEVDFSLPTFVDGAAYLSRRTAGIKRLEDLAGRKIAVAAGTTTE
                     RTIAQVLKQYKLDAQLVKVSDHQQGIAAMVREQVDAYASDRSLLIGLALDSGNQNDWS
                     LGPETFSYEPYALMMRRNDADFRLAVNRELARLSRSREIYGIYDRWFGFLAKPGPRLE
                     SMYLLNALPE"
     gene            1472..2473
                     /locus_tag="PLL14_01520"
     CDS             1472..2473
                     /locus_tag="PLL14_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HOG02625.1"
                     /translation="MNHQIPAIALLLASILTAIPAHAETTRYRGFCDASAAVALGPGH
                     FVVADDDRDILTIYPLGKPDATASVDLGEYLGNRQGTDKARKSDIEGAARIGNRIYWI
                     ASHARNSKGKFQEERQRLFATEVVDSAAGPTLKAATSPPYPRLLQDLAKDARFASLTA
                     ASALPPEAPGGLNIEGLAATPDGQLLIGFRNPLSAGQALLLPLKNPAAVLDQAASPVF
                     GDLVALDLGGRGIRSIEFIASRFVIVAGPFDNGKGSGKGFALYTWSGKGADAPQRVKE
                     VDFGGFHPEALFATGGRNEITVLSDDGNDSCKDQNLPASGKSFRGLSVTLPEPGQRR"
     gene            complement(2499..3284)
                     /locus_tag="PLL14_01525"
     CDS             complement(2499..3284)
                     /locus_tag="PLL14_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG02626.1"
                     /translation="MRPVLAFDIETVPDVEGLRRLHALDQTLSDTEVAELAFQQRRAA
                     SGNDFLPLHLQRVVVISCVLRVGKDFRVWSLAEPEQGEAEIIQRFFDGVEKFTPQIVS
                     WNGGGFDLPVLHYRGLMHGLVAPRYWDLGDGDYPDSREFKWNNYISRYHTRHLDLMDL
                     LAMYQPRASVPLDELAKLIGFPGKLGMDGSKVWGAWQAGRIAEIRDYCETDALNTYLV
                     AVRFRLMRGEFARAEYDAEMAFVRAELERIGKPHWQEFLAAWP"
     gene            complement(3294..4193)
                     /gene="cysM"
                     /locus_tag="PLL14_01530"
     CDS             complement(3294..4193)
                     /gene="cysM"
                     /locus_tag="PLL14_01530"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HOG02627.1"
                     /translation="MDKTLEDYVGGTPLVRLKRLPGAASDARGNRVLAKLEGNNPAGS
                     VKDRPALSMISRAETRGEIKPGDTLIEATSGNTGIALAMAAAVRGYRMILVMPENQSV
                     ERRQTMRAFGAELVLTPKDGGMELARDVAEGMRNEGRGIILDQFANPDNPLAHYEGTG
                     PELWHQTAGRITHFVSSMGTTGTIMGVSRYLKEQNPGVCIVGCQPEDGSQIPGIRKWP
                     EAYLPKIFDRTRVDRVESVAQEEAEEMTRRLASEEGIFAGISSGGSLVVALRLAGELE
                     NSTIVSIVCDRGDRYLSTGVFPA"
     gene            complement(4186..5190)
                     /gene="rfaD"
                     /locus_tag="PLL14_01535"
     CDS             complement(4186..5190)
                     /gene="rfaD"
                     /locus_tag="PLL14_01535"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOG02628.1"
                     /translation="MYMIVTGAAGFIGSNLVKALNERGVRKIIAVDNLTRADKFRNLV
                     DCDIADYIDKEEFLDRLIAGDFDGDVDAVFHQGACSDTTESDGRYLMENNYRYSSALL
                     DWCLDQEVPLLYASSAATYGGGGVFREERAHEAPLNVYGYSKFLFDQIVRQRLAVAGS
                     FNSQVVGFRYFNVYGPRESHKGRMASVAFHHYHQFRGQGKVRLFRGCDGYGDGEQRRD
                     FVHVGDVVRVNLFFLDHPEKSGIFNVGTGRAQTFNELSMATVNGCRALAGDAALPLGE
                     LVEQGLIEYMPFPDDLRGKYQSFTEADLSRLRKAGYDAPFAGVEEGVAQYVEWLSRHG
                     "
     gene            complement(5199..6131)
                     /gene="rfaE1"
                     /locus_tag="PLL14_01540"
     CDS             complement(5199..6131)
                     /gene="rfaE1"
                     /locus_tag="PLL14_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOG02629.1"
                     /translation="MHLADLSAARLLIVGDVMLDRYWFGEVARISPEAPVPVVKVGRS
                     EERPGGAANVARNVAALGARVALLSVVGEDEAGRSLQRLLTASGIDVSLHLDPAISTT
                     VKLRVIGRQQQLLRIDFETAPSHEVLRAKLVEFESRLLDCDAVILSDYGKGGLTHIGE
                     MIRLARAAGKQVLVDPKGDDFSKYAGATIVTPNRAELREVVGRWKDDEDLARKAEVLR
                     RGLALEALLVTRSEEGMSLFQAAGALHEKAVAREVFDVSGAGDTVIATLAVMLACGAS
                     WQDAVHVANIAAGVVVGKLGTAVVSRDELAAAMK"
     gene            complement(6218..7387)
                     /gene="lapB"
                     /locus_tag="PLL14_01545"
     CDS             complement(6218..7387)
                     /gene="lapB"
                     /locus_tag="PLL14_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HOG02630.1"
                     /translation="MIEFEYWQLLLFPLFFGLGWAAARVDIRHLVKESRALPRSYFQG
                     LNFLLNEQPDRAIEAFVEAVQVDPQTIELHFALGSLFRRRGETERAIRMHQNLVGRDD
                     LPQDLKLQALAELGQDYLKAGLLDRAEEIFDKLRDSDLAEDAKRNLLEIYQLEKDWEK
                     AIAIASELPDFASHREIAEYYCELAAAEIMRSRRDLAAVYLQTAFERNRKCVRASLLL
                     GDIQFQQDQLLAAIETWQRIEQQDPPYLALVAQRLLDAFRKLDRREEGLQLLRGYLEH
                     YPSLDLLDVVFQLVLEGDGPEAAYKLVRDELKRNPTLLGFDKLLEARLLVTAPENRPD
                     LDLAKGIVQGYTRRLARYRCDNCGFKARQFYWRCPACGGWETYSPKRTEEFDLTP"
     gene            complement(7578..7871)
                     /locus_tag="PLL14_01550"
     CDS             complement(7578..7871)
                     /locus_tag="PLL14_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOG02631.1"
                     /translation="MTKSDLIAQLAGRFPQLVAKDADLSVKMILDAMAEALSKGDRIE
                     IRGFGSFALNYRPPRVGRNPKSGEKVEVPEKWVPHFKAGKELRERVDGSLESI"
BASE COUNT         1522 a         2674 c         2353 g         1487 t
ORIGIN      
        1 tttcgagcaa gcggacttcg cactcgtcta ttttgccgtc gggggcttct ttgcgacctt
       61 cgtcttcgcc atcagtgtaa ttgccttgcc actgatgctc gatcgcaaga ccgatgcgat
      121 caccgcggcg atcgccagcc tcgtcgcctg cggccgcaac cccgggacga tgctgctctg
      181 ggccgcgtgt atcgtggtcc tggtgagcgt gggcttcgcg accttgttct tcggcctgat
      241 cgtgaccatg cctctggtcg ggcacgcgac ctggcacgcg tatcgggatc tggtcgacaa
      301 cgacgactcc ccggagccag ggaacaggcg ctgagccacg aggcaatgcc gcgctgcctg
      361 ctcggctact ccggcaaagc gttcagcagg tacatgcttt cgaggcgtgg acccggcttg
      421 gccagaaagc cgaaccagcg gtcgtagatg ccgtagatct cgcggctgcg cgagagcctg
      481 gccagttcac gattcaccgc caggcggaag tcggcatcgt tgcggcgcat catcaaggcg
      541 taaggctcat aggagaaggt ttccggacct agcgaccagt cgttctggtt gcccgagtca
      601 agcgcgagcc cgatcagcag ggatcgatcg gaggcgtagg catcgacctg ttccctgacc
      661 atcgccgcaa tcccttgctg gtggtcgctg accttgacca actgcgcatc cagtttgtac
      721 tgtttgagaa cctgggcaat cgtccgctcg gtcgtcgtac cggcggcgac ggcaattttc
      781 ctgccggcca ggtcttcgag ccgcttgatg cccgccgtcc ggcgtgacag gtacgctgcg
      841 ccatcgacga aagtcggcag gctgaagtcg acttcctgca tgcgcgtcag cgagctggtc
      901 gtcgatccgc attcgagatc gatctcgccg gacttgagtt tggcgatacg gttttccggg
      961 gtgactgcga cccactgcaa ctgcagttcc tccagaccca gctgcttgac gatcgctgcc
     1021 gccacccggg tgcacaagtc caccgaataa ccccagggct ggttatcgtc gccggtatag
     1081 gagaaaggga tcgatgtctc gcgatagccg agggcaatcg cttgcgaagc cttgatcttg
     1141 tcgagtgtcc cggcagcgtg caccggcacg gccaggcaca ggcctacagt cgtcgaggcc
     1201 aatagaatcc agcgacgcag tctcatacga tttctcccca acggtcatgg atttgtcaga
     1261 caccccggat gatgaaagcc atgaccgttt ccctcacccc cgacccctct cccgcctgca
     1321 ggcgaggcga gattcgtcag tcgctggcgc gactttcaca gcatacgtgg cttctccacg
     1381 ggcgatgatt ttgaccgatg tacggcaatt tggaaatcgc tttacgggat aaagctatac
     1441 tgcacggcca ctcgcgagga aaccaccgac catgaaccac cagattcccg ccatcgccct
     1501 gctcctggca tccatcctga ccgccatccc cgcgcacgcg gaaaccaccc gctaccgcgg
     1561 cttctgcgat gcttccgcgg ccgtcgcact cggccccgga cacttcgtcg tggccgacga
     1621 cgatcgcgat atcctgacca tctacccact cggcaagccg gacgcgacgg cgagcgtcga
     1681 cctgggcgaa tacctcggca atcgccaggg tacggacaag gcgaggaagt cggacatcga
     1741 gggcgccgcg cgcatcggca accgcatcta ctggattgcc tcgcatgcgc gcaatagcaa
     1801 gggcaagttc caggaggaac ggcaaagatt atttgccacc gaggtagtgg acagtgctgc
     1861 cggtccgacc ctgaaggcgg cgacctcgcc accctaccct cgcctgctgc aagaccttgc
     1921 caaagacgcc cgctttgcct ccctcaccgc ggccagcgcc ttgccaccgg aagccccagg
     1981 tggcctgaac atcgaaggcc ttgccgcaac acccgacggg caactgctga tcggcttccg
     2041 caatccactc tcagcaggcc aggccttgct gctgccgctg aagaaccctg cggcggttct
     2101 cgaccaggcc gcttcgccgg ttttcggtga cctcgtcgcc ctcgacctcg gtggcagggg
     2161 cattcgcagc atcgaattca ttgcatcgcg cttcgtgatc gtcgccgggc cgtttgacaa
     2221 cggcaagggc agtggcaaag gctttgccct ctacacctgg tccggcaaag gtgctgatgc
     2281 gccgcagcgc gtcaaggaag tggacttcgg cggattccac ccggaggcac tcttcgccac
     2341 tggcggccgc aacgagatca ctgttctcag cgacgacggc aacgacagtt gcaaggacca
     2401 gaatctgccg gccagcggaa agtcgtttcg tggcctttcc gttaccttgc cggaacccgg
     2461 ccagcgtcgc tgaccctgcc agccctggcg aagccatact acggccaggc ggccaggaac
     2521 tcctgccagt gcggtttgcc gatccgctcc agttcagcgc gcacaaaggc catctcggcg
     2581 tcgtattcgg cacgtgcgaa ctcgccgcgc atcaggcgga aacgcaccgc gacaaggtag
     2641 gtgttgagcg cgtcggtttc gcaatagtcg cggatttctg caatcctgcc cgcttgccag
     2701 gccccccaga ccttgctgcc gtccatgccg agtttgcccg ggaaaccgat cagcttggcc
     2761 aactcgtcaa gcggcacact ggcccgcggc tgatacatcg ccagcaggtc catgaggtcg
     2821 aggtgacgcg tgtggtagcg gctgatgtag ttgttccact tgaactcgcg gctgtccggg
     2881 tagtccccgt cgccaaggtc ccagtaacgc ggtgcgacca gcccgtgcat cagcccgcga
     2941 tagtgcagga ccggcaaatc aaaaccaccg ccgttccagg acacgatctg cggcgtgaat
     3001 ttctcgacgc cgtcgaagaa acgttgaatg atctcggctt cgccctgctc gggctcggcc
     3061 agcgaccaga cgcggaaatc cttgccaacg cgtagcacgc aggaaatcac caccacccgc
     3121 tgcagatgca gcggcaggaa gtcattgccg ctcgccgcgc gccgctgctg aaaagccagc
     3181 tcggcgacct cggtgtcgga cagggtctga tcgagcgcat gcagcctgcg caaaccctcg
     3241 acgtccggaa cggtttcaat gtcaaaggcg aggaccggcc gcatcgtgcg ccctcaggcc
     3301 ggaaagaccc cggtcgacag ataacggtca ccgcggtcgc agacgatact gacgatggtc
     3361 gagttttcga gttcgccggc cagtcgcagg gcgacgacca gcgagccacc ggaagagatg
     3421 ccggcaaaga ttccctcttc gctggccagg cgacgggtca tttcttccgc ctcctcctgc
     3481 gcgaccgact ccacgcgatc cacgcgcgtt cggtcaaaaa tcttcggcag gtaggcttcc
     3541 ggccatttgc gaatgccggg gatctgcgaa ccgtcttcgg gctggcagcc gacgatgcaa
     3601 acgccggggt tctgctcctt gaggtagcgt gaaaccccca tgatcgtccc ggtcgtgccc
     3661 atgctgctga cgaaatgcgt gatccgcccc gccgtctggt gccacagttc cgggccagtc
     3721 ccttcgtagt gcgccagcgg gttgtccggg ttggcgaact ggtcgaggat gatgccgcga
     3781 ccctcgttgc gcatgccctc ggcgacgtca cgcgcgagtt ccatgccgcc atccttgggc
     3841 gtcaacacca gctcggcgcc gaaggcgcgc atcgtctggc gtcgttcgac actctgattt
     3901 tccggcatca ccaggatcat ccggtagcca cgcacggcag cggccatcgc cagggcaatg
     3961 ccggtattgc cggaagtggc ctcgatcagc gtgtcaccgg gcttgatctc gccacgcgtc
     4021 tcggcacgcg agatcatcga caaggccggg cggtccttca ccgacccggc cgggttatta
     4081 ccctccagct tggccagtac gcggttgcca cgcgcgtcgc tggccgcccc cggcaggcgc
     4141 ttgagacgca ccagcggagt acccccgacg tagtcctcca gcgtcttatc catgccggct
     4201 caaccattcc acgtactgcg cgacaccttc ttcgacgccg gcaaacggtg catcgtagcc
     4261 cgccttgcgc agcctcgaca gatcggcctc ggtgaaactc tgatacttgc cccggaggtc
     4321 atcgggaaac ggcatgtatt cgatcaaacc ctgctccacc agttcgccaa gcggcagcgc
     4381 cgcgtcgcct gcaagcgcac ggcagccatt gacggtggcc atcgaaagct cgttgaaggt
     4441 ttgtgcgcgg cccgtgccga cgttgaaaat gcctgatttt tccggatgat cgaggaaaaa
     4501 caggttgacc cgaacgacgt cgccgacatg cacgaaatcg cgtcgctgct cgccatcgcc
     4561 ataaccatca caaccccgaa acagccggac cttgccctgg cctcggaact gatggtaatg
     4621 gtgaaacgcc accgaagcca tgcgtccctt gtgcgactca cgcggaccat agacattgaa
     4681 gtagcgaaag ccgacgacct gcgagttgaa cgagccggcg accgcgaggc gctgtcggac
     4741 gatctggtcg aagaggaact ttgaatagcc gtagacgttc agcggcgcct cgtgcgcccg
     4801 ctcttcccgg aacacgccac cgccgccgta ggtcgccgca ctcgacgcgt aaagcagcgg
     4861 cacctcctgg tcgaggcacc agtcgagcaa cgccgacgag tagcggtagt tgttttccat
     4921 caggtagcgc ccgtcgctct cggtcgtatc ggagcatgcg ccctggtgaa aaacggcatc
     4981 gacgtcacca tcgaaatcgc cggcgatcag gcggtcgagg aattcctcct tgtcgatgta
     5041 atccgcgatg tcacaatcga cgaggttccg gaacttgtcg gcacgggtca ggttgtcgac
     5101 cgcaatgatc tttcgtacgc cgcgctcatt gagcgccttg acgagattcg agccgatgaa
     5161 gccggcagcg ccggtaacga tcatgtacat cccttacctc acttcattgc cgcggcaagt
     5221 tcgtcgcgac tgaccaccgc cgtgccgagc ttgcccacca ccacgcctgc ggcgatgttc
     5281 gccacatgca ctgcgtcctg ccaactggcg ccacacgcga gcatcaccgc gagggtggca
     5341 atgaccgtgt cgccggcgcc ggaaacgtcg aacacctctc tcgccacggc cttttcgtgc
     5401 agcgcgcccg ccgcttgaaa aagcgacatc ccttcctcgc tgcgggtgac cagcagggcc
     5461 tccagcgcca acccgcggcg cagtacctcg gccttgcgcg cgagatcctc gtcatccttc
     5521 cagcgaccga ccacttcccg caattcggcg cggttcgggg tcacaatggt cgcgccggca
     5581 tacttcgaga aatcgtcccc cttcgggtca acgaggactt gcttgccggc tgcccgcgcc
     5641 agacggatca tttcgccgat gtgcgtcaag ccacctttac catagtcgga aaggatcacc
     5701 gcgtcgcagt ccagcagacg ggattcaaac tcgaccagct tggcgcgcaa cacctcgtgt
     5761 gagggcgccg tctcgaaatc gatacgcagc aactgctgct gccggccaat cacgcgcagc
     5821 ttgacggtcg tgctgatcgc cgggtcgagg tgcaggctga catcgatgcc actcgccgtc
     5881 aacaggcgct gcaggctacg ccccgcctcg tcttcgccga cgaccgacaa cagggcaacg
     5941 cgtgcgccca gagcggccac gttgcgcgcc acgttggcgg cgccgcccgg gcgctcttcg
     6001 ctgcgcccga ccttgaccac cggcaccggc gcctcgggcg aaatgcgggc cacctcgcca
     6061 aaccagtaac ggtcgagcat cacgtcgccg acgatgagca gacgtgcggc cgacaggtca
     6121 gcgaggtgca tcgctgcggg gctcggcaag gcgaggaatg atacccggac agcgcctgta
     6181 gcgcaggtcg cgcatgacga ctggcaagct ggatctccta tggtgtcaga tcgaattctt
     6241 ccgtccgttt tggcgaatag gtctcccaac cgccgcaagc cggacagcgc caatagaact
     6301 ggcgcgcctt gaaaccgcaa ttatcgcatc gatagcgagc caggcggcgc gtgtagccct
     6361 gcacgatacc cttggccaga tccagatccg gtcgattctc gggtgccgta accaacagtc
     6421 tggcctccag caatttgtcg aatccgagca gtgtcggatt gcgcttcagc tcatcgcgaa
     6481 ccagcttgta cgccgcctcg ggcccatcgc cctcgagcac gagctggaac accacgtcca
     6541 gcaggtccag ggaaggatag tgttccaggt aaccgcgcaa caactgcagg ccctcttcgc
     6601 gtcgatccag tttgcggaag gcgtcgagca aacgctgcgc gaccagcgcc agataagggg
     6661 gatcctgctg ttcgatgcgc tgccacgttt cgatcgccgc aagcagttgg tcctgttgaa
     6721 actgaatgtc cccaaggagc aggctggcac gaacgcattt gcggttgcgc tcgaacgcgg
     6781 tctgcaggta gaccgccgcc agatcgcgcc gcgagcgcat gatttccgcc gccgccagtt
     6841 cgcagtagta ctcggcgatt tcccggtgtg aggcgaagtc gggaagttcc gaagcgatcg
     6901 cgatcgcctt ctcccagtcc ttctcaagct gataaatttc gagcaggttg cgtttcgcgt
     6961 cttcggccag atccgaatcg cgcagtttgt cgaagatttc ctcggcgcgg tcgagcaacc
     7021 cggccttgag atagtcctgc cccagttcag caagcgcctg cagtttcaga tcctgcggaa
     7081 gatcatcgcg cccgacgaga ttctggtgca tgcggatggc gcgctcggtt tctccgcggc
     7141 ggcggaacag gctgccgaga gcaaaatgca gttcgatggt ctgcggatcc acctggacgg
     7201 cttcgacaaa cgcctcgatc gcacgatccg gctgctcgtt gagcaggaag ttcagtccct
     7261 gaaaatacga acgcgggaga gccctggact ccttgaccag atgccggatg tcgacccgtg
     7321 ccgctgccca ccccaagccg aagaacagcg ggaagagcaa tagttgccag tattcgaact
     7381 cgatcatgca ctcaactctg tggtttcagg caacgcccga accgctctcc gggcggcttc
     7441 acaggtctgg cgttcgactt gccgaccacc ctcacccaca actcccgatt cgggctggcc
     7501 aaggtacgca tcaatcacct ttcgacaaga agcgggcggc acctcgccgc ccgcaatccg
     7561 ccacacggtc tgagctctca gatcgattcc agcgagccgt cgacgcgctc ccgcaattcc
     7621 tttcccgcct tgaaatgcgg cacccacttt tccgggacct caaccttctc acccgatttc
     7681 ggattgcggc cgacacgcgg tggccggtag ttcagggcaa aactcccgaa gccgcgaatc
     7741 tcgatgcggt cacctttgga caaagcctcg gccatcgcat cgagaatcat ctttaccgac
     7801 aaatccgcat ctttggcgac cagttgaggg aaacgcccgg ccagttgagc gatgagatcc
     7861 gacttggtca tcctcctcga accctattgg ggattgttga gcttggcttt gagcaaggca
     7921 cccagattcg tcgttcccga actggcgcta ctgtcggcag aaaacttctg catcgcctct
     7981 tgctgctcgg cctgctcccg ggccttgatc gacaggctga tcgagcgcgt cttgcg
//
LOCUS       DAOSIT010000031         7734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_8690_length_7734_cov_10.235968, whole genome shotgun sequence.
ACCESSION   DAOSIT010000031 DAOSIT010000000
VERSION     DAOSIT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8690_length_7734_cov_10.235968"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..901)
                     /locus_tag="PLL14_01555"
     CDS             complement(38..901)
                     /locus_tag="PLL14_01555"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG02632.1"
                     /translation="MAGKKALFVGINKFANYSQFTLNGCVNDARDMAALYKDLLGFKT
                     TELSVLTDAQATKANIMARLNTMVADAKAGKLNYLVFSLSSHGTQMNDSSGDEPDGKD
                     EAFVPHDIAEKDGAWDPAHIISDDELHDLFVQLPANVLLEVYLDTCHSGTGLRGAEFG
                     LHAPRARFIAPPEHEFGTKFASVRGFTLDRPAAGKKGSAVAEKAPVAGANHVLWTGCK
                     ADQTSADAYFSGRYNGAFTYYLIKVVRESQNKLSRKETVTRMRKLMKSGFAQSPQLEG
                     NASNRARAIVY"
     gene            complement(1046..1627)
                     /locus_tag="PLL14_01560"
     CDS             complement(1046..1627)
                     /locus_tag="PLL14_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HOG02633.1"
                     /translation="MMVPPISRFPVPALDQLPEDVRSRILAVQEKAGFVPNVFLALAH
                     RPDEFRAFFAYHDALMEKESGLSKAEREMIVVATSGANQCQYCVVAHGAILRVRARNP
                     LIADQVAVNYRKADLSARQRAMLDFALKVSLHAAEVGDADFASLHAHGFTEQDIWDIG
                     AVAAFFAMSNRLANMTSMRPNDEFFLMGRLPKA"
     gene            complement(1628..2059)
                     /locus_tag="PLL14_01565"
     CDS             complement(1628..2059)
                     /locus_tag="PLL14_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG02634.1"
                     /translation="MNSLGGYNIEDLQTGMSASIAKTVTEADIVLFAGITTDVNPMHL
                     NEEWAKTTQFGGRIAHGMLSAGFISAVLANKLPGPGTVYLGQTLKFKAPVRIGDTVTA
                     TVTVRELNVSRRRCVMDSVCTVNGKTVIEGESTLYCTTGEA"
     gene            2136..3014
                     /locus_tag="PLL14_01570"
     CDS             2136..3014
                     /locus_tag="PLL14_01570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOG02635.1"
                     /translation="MSRSSDFDSLVPAVPFSRRSFMISALGAGFALSVQPVAAQSVIS
                     TDSVDLNAGEIKIPAAGGEMPAYRAQPAQGNQWPVILVVQEIFGVHEHIRDICRRLAK
                     LGYLAIAPELYARQGDPRQYSNVQELLDKVVSKIPDAQVMADLDACVAWAKAHDGNVG
                     RLGITGFCWGGRIVWLYAAHNPHLKAGVAWYGRVVGPSSELTPKQPIDIAARLYAPVL
                     GLYGGADQGIPLDSLEQMRKALVMAANPPSKASTIHVYEQAPHAFHADYRPSYRKSEA
                     EDGWRRLQAWFKQNGV"
     gene            3280..>7734
                     /locus_tag="PLL14_01575"
     CDS             3280..>7734
                     /locus_tag="PLL14_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG02636.1"
                     /translation="MAKAHGEEAHPVRNDERPFIVGIGASAGGLEALSLLLPGLPKNL
                     GLSYVVVQHLSPTYRSMMSQLLGRETTMPVRDIEDGMLPEPNTVYITPPNRNLTLVAG
                     HFRLVEPARESLPKPSVNRFFASLAEEVGESTIGIILSGTGSDGAAGIHAIKAAGGFT
                     FSQDPETAKYNGMPQSAIDTGSVDWILPPENMGAEISLIVLNRGLIPVATQAASAPAT
                     LKTLLGKVRARTKVDFSQYKEPTLWRRIERRMAANHVSTLLDYLQVVDQTPVELDKLC
                     KDILISVTAFFRDTEAFLRLDKVVADILANKQPGDDIRVWVAGCATGEEAYSLAILFS
                     ERLGASFDQYRLQIFATDIDLDAMALARRGVFAASSLAHMDRARLRANFTPHGDRYEI
                     NKNLRDVVIFARQDLVQDPPFLRLDLVSCRNVLIYFQSELQARLLSVFHYALNPGAYL
                     FLGKSEGIFQQEALFGVVDKEGRLYRRHGVGARLPLLRSEMQLPAAGLNQHQRVSKPT
                     TPLGFENILLEAAGRHFIPTSILINSKFEIRHIHGDASRLLNVSPGRPAFDLISLIRR
                     ELRTEVQVLMRQAQVKQAVAPGRPRHIKALDPNRGVRLSVHPVLAAGNEALFMVCIEW
                     IAPPAGKNTVEDSTNVTDKELEDELAATREHLQTLVEELETSNEEMQALNEEIQASNE
                     EMQASNEELEASNEELQSTNEELATVNEELQIKTAETQELNIELECIQNSVDYPLLVL
                     DRNACLLRFNSGAARLFKLGAAQVGRHLRDLPLAPDMPDLVADSQKVIDTQNALDRQI
                     VNANRRHYALHIAPLLRDTQRIAGVIMLFTDNTSLYEIERSARETQVRLLAVMNNSVS
                     LMAVKDASGRYQFANPKFEQTFGFEAGQAIGKTDRQLFPEAVCDLFRDSEIEAMRLRR
                     EIEREEALPLAGGDRHFLAVRFPLFDDDGAITGLCFQATDITARKRAEERLRLAAMVF
                     ERASEGVMVTDTEQRILTVNDAFTTLTGFPRKEVVGKKPSILRSERTAPEVYVDMWDR
                     VNRLGVWQGEIWNCRKNGEQFLEWLSINTVKDKNGKVVNYVGMFSDITKVRESQQRIE
                     YLATHDELTGLPNRALFNDRLHLALARAERSRESIGVVFIDLDNFKVVNDTLGHVTGD
                     KLLKQAAMRLLDCVRAEDTVARLGGDEFVVLLETADRREATLTAERLLSALSASYQFD
                     EHECFISASIGLSMFPEDASDANALMRNADSAMYRAKDHGKNAFRFFTADLAHQATRR
                     LALETGLRRAIENKELFIYYQPQIKLENHTVVGAEALVRWHCNGELVEPVVFIPVAEQ
                     SNLIVAIDEWVLGEACRQIVAWDQDGLPPVRISVNISARHFRKEGMAADLMRIVNAHG
                     VAPQRLCIEITEGVLMDFDRAQRMLAELVAFGVMISIDDFGTGFSSLSYLKRFPIHEL
                     KIARSFIDGISTSADDRAIGSAIISLARHLGMSVVAEGVEMVDQHSELD"
BASE COUNT         1522 a         2427 c         2327 g         1458 t
ORIGIN      
        1 gacttgcgca gcccccctgc cctgcgccgc ggcgaattca atagacgatc gcccgcgcgc
       61 gattgctggc attgccttcc agttgtgggc tctgggcaaa accggatttc atcagcttgc
      121 gcatcctggt gaccgtttcc ttgcgcgaca gcttgttctg cgactcgcga acgaccttga
      181 tcaggtagta ggtgaaggcg ccgttatagc gaccactgaa ataagcgtcg gcgctcgttt
      241 gatcggcctt gcagccggtc cagaggacat gattggcgcc ggcaaccgga gctttctcgg
      301 caacggcaga tccctttttg ccggcagccg gacggtcgag cgtgaaaccc cgcacgctgg
      361 cgaacttcgt gccgaactca tgctcgggtg gtgcgatgaa acgcgccctg ggcgcgtgca
      421 gcccgaactc cgcgccgcgc aaaccggtgc cgctgtgaca ggtatcgagg tagacctcca
      481 gcagcacatt ggccggcaac tgcacgaaca gatcgtgcag ttcatcatcg ctgatgatgt
      541 gcgccggatc ccaggcgccg tccttttcgg caatgtcgtg cggtacgaag gcttcgtcct
      601 tgccgtccgg ttcgtcgccg ctgctgtcgt tcatctgggt gccgtgtgag gacaggctga
      661 aaaccagata attgagcttg ccggccttgg catcggcaac catggtgttc agcctggcca
      721 tgatgttcgc cttggtagcc tgtgcgtcgg tgagcacgct caactcggtg gtcttgaaac
      781 ccaacagatc cttgtacagc gccgccatgt ccctggcgtc gttgacgcag ccattgagcg
      841 tgaattgcga gtagttggca aacttgttga tgcccacgaa gagcgctttc ttcccagcca
      901 tgagcttgtc tccttccagt accgcagcca ataaggttga cggtggtgcg gttgccaccg
      961 ctatggcatt gtgcggcaat cgtcgaccgg ccgcaacgca gtgttgctgt tgccataaca
     1021 ggcggcgggc gggtgcagtt tcgcttcagg ccttgggcaa ccggcccatc agaaagaatt
     1081 cgtcgtttgg ccgcatcgaa gtcatgttcg ccagccggtt cgacatcgcg aagaatgcag
     1141 ccactgcacc gatgtcccag atgtcctgtt ccgtgaagcc atgcgcgtgc agcgaagcga
     1201 aatcggcgtc gccaacctcg gccgcgtgca gcgaaacctt gagcgcgaag tcgagcatcg
     1261 cgcgctggcg ggcgctgagg tcagccttgc gataattgac cgccacctgg tccgcgatca
     1321 gcgggttgcg ggcgcgcacc cgcaggatcg cgccgtgcgc cacgacgcaa tactggcatt
     1381 ggttggcgcc gctggtggcg acgacgatca tttcccgctc ggccttgctg agaccgctct
     1441 ccttttccat caacgcatcg tgataggcaa agaaagcgcg gaactcgtcg gggcgatgcg
     1501 ccagcgcaag gaagacattg ggaacgaaac cggctttctc ctgcacggcg aggatgcggc
     1561 tgcgcacatc ctccggcagt tgatcgagtg ccggcaccgg gaagcggctg atcggcggca
     1621 ccatcaatca ggcctcgccg gtggtgcagt acagcgtcga ctcgccttcg atgaccgtct
     1681 tgccgttaac ggtacatacc gagtccatca cgcaacgccg ccggctcacg ttcaactcac
     1741 ggacggtgac cgtcgcggtg acggtgtcgc cgatacgcac cggcgccttg aacttgagcg
     1801 tctgaccgag atagacggtc cccggtcccg gcagcttgtt ggccaatacg gcggaaatga
     1861 agccggccga aagcatgcca tgcgcgatgc gaccgccgaa ctgggtggtc tttgcccatt
     1921 cctcattgag gtgcatcggg ttgacgtcgg tcgtaatgcc ggcgaagagg acgatgtccg
     1981 cctcggtaac cgttttggca atgctcgcgc tcatgccggt ttgcagatct tcgatgttgt
     2041 agccgccaag tgaattcatc actatctcca tggtccgtgg gttgcagatc gtgccatgat
     2101 acacaatcga cgtatccatc agaggggaga tattcatgag ccgcagcagt gattttgaca
     2161 gccttgtacc cgccgttcca ttcagccgtc gcagcttcat gatcagcgcc ctgggcgccg
     2221 gctttgccct gtcggtgcaa ccggtggcgg cgcagagcgt gatcagcacc gacagcgtcg
     2281 acctcaacgc cggcgagatc aagattcctg cggccggtgg cgagatgccg gcctaccgcg
     2341 cgcaacctgc gcagggtaac cagtggccgg tgattctggt ggtgcaggag attttcgggg
     2401 tgcatgaaca tatcagggac atctgccgtc ggctggcgaa actcggttac ctggcgatcg
     2461 cgccagaact ctacgcccgt cagggcgacc cgcgccagta cagtaacgtc caggaactgc
     2521 tcgacaaggt ggtgagcaag atcccggacg cacaggtgat ggccgatctc gacgcctgcg
     2581 tggcctgggc caaagcgcac gatggcaacg tcggtcgcct ggggatcacc ggcttctgct
     2641 gggggggacg catcgtctgg ctgtacgccg cgcacaatcc acatctgaag gccggtgtcg
     2701 cctggtatgg tcgggtcgtc ggtccgtcca gcgaactgac cccgaaacag ccgatcgaca
     2761 tcgccgccag actgtacgca ccggtcctgg gactctacgg aggagccgat cagggaatcc
     2821 cgctcgacag cctggagcag atgcgcaagg cactcgtcat ggctgccaat ccgcccagca
     2881 aggcttcgac gatacacgtc tatgagcagg ccccgcacgc ctttcatgcc gattaccggc
     2941 cgagctaccg caagagcgag gccgaagatg gctggcgccg cctgcaggcc tggttcaaac
     3001 agaacggcgt ctgattccgg cagatgccgc ttaggaatac aatgcgttgc cgcccgagct
     3061 gagccgaaga gttcctgcga cgcccccgcc ggcctgaagg ggaaccagat ctggaagaca
     3121 caggtccact gctgggtttt ccgacggccg tccagcgtcg gtcccttcca gcttggacag
     3181 acgcgaagcg cattacaggg cagaacaacc aaaacgatga ccatcagcaa cgcagaagaa
     3241 cccaaaaagc ggcgcagtgt ttcacgaccg ccgttgcccg tggctaaggc ccatggcgag
     3301 gaggcccatc cggtcaggaa cgacgaacgg ccattcattg tcggtatcgg cgcctcggcc
     3361 ggtggtctgg aagcactcag cctgctgttg ccgggcctgc cgaagaatct cgggctgagc
     3421 tacgtggtgg ttcagcatct gtcgccgacc taccgcagca tgatgtcgca gttgctcggt
     3481 cgcgagacga cgatgccggt ccgggacatc gaggacggca tgctgccgga gccgaacacg
     3541 gtttacatca ccccaccgaa tcgcaacctg acgctggtgg ccggccattt ccgcctcgtc
     3601 gaaccggcgc gcgaatcgct gcccaagcct tcggtgaacc gcttttttgc ctcgctcgcc
     3661 gaagaggtcg gcgaatcgac tatcggcatc atcctctccg gtaccggttc tgatggtgcg
     3721 gccggcatcc atgcgatcaa ggcggcgggt ggattcacct tctctcagga tcccgaaacc
     3781 gctaaataca atggcatgcc ccagtcggca atcgacaccg gcagtgtgga ctggattcta
     3841 cctcccgaga acatgggcgc cgagatcagc ctgatcgtcc tcaaccgcgg cctgattcct
     3901 gtcgccacac aggcggcgag cgccccggcg acgctgaaaa cgctgctcgg caaggttcgc
     3961 gcccgtacca aggtggattt ctcgcagtac aaggagccga ccctgtggcg gcgcatcgag
     4021 cggcgcatgg ctgccaacca cgtcagcacg ctgctcgatt acctgcaggt ggtcgaccag
     4081 acgccagtcg aactcgacaa gctgtgcaag gacattctga tctcggtcac cgcctttttt
     4141 cgcgataccg aagcttttct tcggctcgac aaggtcgtgg ccgacattct cgccaacaag
     4201 caacccggtg acgacatccg cgtctgggtc gccggctgcg ccaccggcga ggaagcgtat
     4261 tcgctggcca tcctgttttc cgaacgtctg ggtgcctctt tcgaccaata tcggctgcag
     4321 atcttcgcca ccgacattga cctggatgcg atggcgctgg cgcggcgcgg cgtttttgcc
     4381 gcgtccagcc tggcgcacat ggaccgcgcc cgcctgcgtg ccaacttcac accgcacggc
     4441 gaccgctacg agatcaacaa gaacctccgc gacgtggtga tcttcgcccg tcaggatctg
     4501 gtgcaggacc cgccctttct tcgcctggat ctggtcagtt gccgtaatgt cctgatttat
     4561 tttcagagcg agttgcaggc acggttgctg tcggtgttcc attacgccct caacccgggt
     4621 gcttacctgt tcctcggcaa gtccgaaggc atcttccagc aggaagcgct gttcggcgtg
     4681 gtcgacaagg aaggtcgact ttaccgtcgt cacggcgtcg gcgctcgtct gccgctgcta
     4741 cgttccgaga tgcaactccc tgccgcgggc ctcaaccagc accagcgcgt cagcaagccg
     4801 acaacgccgc tgggtttcga aaacatcctg ctcgaggccg ccgggcgcca tttcatcccg
     4861 accagcatcc tgatcaacag caagttcgaa attcgccata tccatggcga cgccagccgc
     4921 ctgctcaacg tttctcccgg ccgaccggcc ttcgacctga tctcgctgat acgtcgcgag
     4981 ttgcgcaccg aagtgcaggt cctgatgcgc caggcgcaag tcaaacaggc cgtggcaccc
     5041 ggacgtcccc ggcacatcaa ggcgctcgac ccgaatcgcg gcgtccgcct ttcggtgcat
     5101 ccggtattgg ctgccggtaa tgaagcgctg ttcatggtct gcatcgagtg gatcgcaccg
     5161 ccggccggca agaacaccgt cgaggacagc accaacgtca ccgacaagga actcgaggac
     5221 gaactggcgg ccacgcgcga acacttgcag acgctggtcg aggaactcga aacctcgaac
     5281 gaggaaatgc aggcactcaa cgaggagatc caggcttcaa acgaggaaat gcaggcctcc
     5341 aacgaggaac tcgaagcgtc caacgaagaa ctgcaatcga ccaacgaaga actggcaacg
     5401 gtcaacgaag aactgcagat caagaccgct gaaacgcagg aactcaatat cgaactggag
     5461 tgtatccaga acagtgtcga ctatcccttg ctggtgcttg accgcaatgc ctgcctgttg
     5521 cgcttcaaca gcggtgctgc acggctcttc aaactgggcg cggctcaagt cggtcgtcat
     5581 ctgcgggatc tgcctctggc cccggacatg ccggatctgg tggccgacag tcagaaggtg
     5641 atcgacaccc agaatgcact cgaccgccag atcgtcaatg ccaaccgtcg ccactacgca
     5701 ctgcacatcg ctccgctgct gcgcgacacg caacgcattg ccggagtcat catgctcttt
     5761 accgacaaca ccagcctcta cgaaatcgag cgctcggcgc gcgaaacgca ggtcaggtta
     5821 ctcgcggtaa tgaacaattc ggtatcgctg atggcggtca aggacgcttc cgggcgttac
     5881 cagtttgcca accccaagtt cgagcagacc ttcgggtttg aggccggaca ggcgatcggc
     5941 aagaccgatc ggcaactttt tcccgaagcc gtgtgcgatc tgttccgcga cagcgagatc
     6001 gaggcgatgc gtctgcgccg ggaaatcgag cgcgaagagg cgctgccgct ggccggtggc
     6061 gatcgccatt tccttgccgt gcgctttccg cttttcgacg acgacggcgc aatcaccggt
     6121 ttgtgtttcc aggctaccga cattaccgcc cgcaagcgcg ccgaagaacg tttgcgtctg
     6181 gcggcgatgg ttttcgagcg cgcttccgaa ggcgtcatgg tcaccgacac cgagcagcgc
     6241 atcctgaccg tcaatgacgc tttcaccacc ctgaccggtt ttccgcgaaa ggaagtcgtc
     6301 ggcaagaaac cgagcatcct gcgctcggaa agaaccgcgc ccgaggtcta cgtcgacatg
     6361 tgggaccgcg tcaaccgcct cggcgtctgg cagggggaga tctggaattg ccgcaagaac
     6421 ggcgagcagt tcctcgaatg gttgtcgatc aatacggtca aggacaagaa cggcaaggtc
     6481 gtcaactacg tcggcatgtt cagcgacatc accaaggtgc gcgagtcaca gcagcgtatc
     6541 gaatacctgg cgacacacga tgaactgacc gggctgccca accgtgcgct gttcaatgac
     6601 cgcctgcatc tggctctggc gcgtgccgag cgctcgcgcg aaagcatcgg cgtggtgttc
     6661 atcgatctcg acaacttcaa ggtggtcaac gacaccctcg gccatgtcac cggtgacaag
     6721 ctgctcaaac aggcggcgat gcgcttgctc gactgcgtgc gcgccgaaga caccgtcgcc
     6781 cgcctggggg gtgacgagtt tgtggtcctg ctcgaaaccg ccgatcgccg cgaggcgacg
     6841 ctgaccgcag agcgcctgct cagcgccttg agtgccagct accagttcga cgagcacgaa
     6901 tgcttcatca gtgccagcat cggtctgagc atgttccccg aggatgcgtc cgatgccaac
     6961 gcgctgatgc gcaatgctga ctcggccatg tatcgggcca aggatcacgg caagaacgcc
     7021 ttccgcttct tcaccgccga cctggcccac caggcgacgc ggcggctggc actggagacg
     7081 ggcctgcgac gagcgatcga gaacaaggag ttgttcatct actaccagcc gcagatcaaa
     7141 ctggagaacc acacggtggt cggcgccgag gctctggtgc gctggcactg caacggggaa
     7201 ctggtggaac cggtggtctt cattccggtc gccgagcaaa gcaatctgat cgtggccatt
     7261 gacgagtggg tgctgggtga ggcctgtcgg cagatcgtcg cctgggatca ggacggactg
     7321 ccgccggttc gtatcagcgt caacatctcg gcccggcact tccgcaagga gggcatggcc
     7381 gccgacctga tgcgcatcgt caacgcgcat ggcgtcgcac cgcagcgcct gtgcattgaa
     7441 attaccgaag gcgtgctgat ggatttcgat cgcgcccaac ggatgctcgc cgaactggtt
     7501 gctttcggtg tgatgatcag catcgatgat ttcggaaccg ggttttcatc actgtcctac
     7561 ctcaaacgct tcccgatcca cgaactcaag atcgcacgca gcttcatcga cggcatctcg
     7621 accagcgccg atgatcgggc gatcggctcg gcaatcatct cgctggcgcg ccacctcggc
     7681 atgagcgtcg tcgccgaagg cgtcgaaatg gtcgatcagc atagcgaact cgac
//
LOCUS       DAOSIT010000032         7630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_8846_length_7630_cov_9.575050, whole genome shotgun sequence.
ACCESSION   DAOSIT010000032 DAOSIT010000000
VERSION     DAOSIT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8846_length_7630_cov_9.575050"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..363
                     /gene="rpsN"
                     /locus_tag="PLL14_01580"
     CDS             58..363
                     /gene="rpsN"
                     /locus_tag="PLL14_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOG02637.1"
                     /translation="MAKLAVINRGEKRRKTVEKYAKKRSELMAIVADQSRSIEERYEA
                     RLKVQALPRNASPVRLRNRCALTGRPRGVYRKFGLGRGKLREFVMQGEVPGMIKASW"
     gene            379..774
                     /gene="rpsH"
                     /locus_tag="PLL14_01585"
     CDS             379..774
                     /gene="rpsH"
                     /locus_tag="PLL14_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG02638.1"
                     /translation="MSMSDPISDMLTRIRNAQMASKASVAMPSSKVKIAIAQVLKDEG
                     YVEDFAVSPNEGKPILEIGLKYYAGRPVIERIDRVSRPGLRIYRGAGDIPQVMNGLGV
                     AIVSTPKGVMTDRKARASHVGGEVLCFVA"
     gene            784..1320
                     /gene="rplF"
                     /locus_tag="PLL14_01590"
     CDS             784..1320
                     /gene="rplF"
                     /locus_tag="PLL14_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG02639.1"
                     /translation="MSRVAKKPIVVPQGVSVNLSAGHISVQGPLGVLDFAVHSAVSVE
                     QEGDSLLCKPVAGVAQADALSGTVRAVVSNMVTGVSKGFERKLSLVGVGYRAQAQGDT
                     LNLTLGFSHPVAYRLPSGITAATPAQTEIVIKGVDRQLVGQVAAEVRAYRKPEPYKGK
                     GVRYVDEVVAIKETKKKK"
     gene            1334..1687
                     /gene="rplR"
                     /locus_tag="PLL14_01595"
     CDS             1334..1687
                     /gene="rplR"
                     /locus_tag="PLL14_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG02640.1"
                     /translation="MDKKQARLRRARKTRAKIAELKSVRLSVHRSNCHIYAQVISACG
                     SKVLAAASSLEPEVRKMLPNGGDIAAAKTIGVLIAERARSAGIEQVAFDRAGFRYHGR
                     IKALADAAREAGLKF"
     gene            1715..2239
                     /gene="rpsE"
                     /locus_tag="PLL14_01600"
     CDS             1715..2239
                     /gene="rpsE"
                     /locus_tag="PLL14_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG02641.1"
                     /translation="MAKPQSRKPQQSEKPDDGMREKMISINRVTKVVKGGRILGFAAL
                     TVVGDGDGRVGMGKGKSREVPVAVQKAMEEARRNLIKVSLKNGTLQHTVVGHHGASRV
                     MMQPAPEGTGIIAGGAMRAVFDVIGMANVVAKAHGSTNPYNIVRATINGLRSMTTPAE
                     VAAKRGKSVAEILG"
     gene            2243..2425
                     /gene="rpmD"
                     /locus_tag="PLL14_01605"
     CDS             2243..2425
                     /gene="rpmD"
                     /locus_tag="PLL14_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOG02642.1"
                     /translation="MTDKKVKITLVRSLIGTKESHRATVRGLGLRRMHSSSLLEDTPA
                     VRGMINKVSYLLKCEG"
     gene            2427..2861
                     /gene="rplO"
                     /locus_tag="PLL14_01610"
     CDS             2427..2861
                     /gene="rplO"
                     /locus_tag="PLL14_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG02643.1"
                     /translation="MELNSVKGAAGARPDRKRVGRGIGSGLGKTCGRGHKGQKSRAGG
                     FHKVGFEGGQMPLQRRLPKRGFKSMTGERNFEVRLSEIARLPVDEIDLATLIQASLVS
                     QHALSAKVVLSGEIHRKVTLKGVAATAGARAAIEAAGGSVSA"
     gene            2885..>3222
                     /gene="secY"
                     /locus_tag="PLL14_01615"
     CDS             2885..>3222
                     /gene="secY"
                     /locus_tag="PLL14_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437564.1"
                     /note="forms heterotrimeric complex in the membrane; in
                     bacteria the complex consists of SecY which forms the
                     channel pore and SecE and SecG; the SecG subunit is not
                     essential; in bacteria translocation is driven via the
                     SecA ATPase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG02644.1"
                     /translation="MVTSSSPISKGGKFGDLKRRLWFLLGALVVYRIGSHIPVPGIDA
                     QVLSELFNKQQGGILGMFNMFSGGALSRFTIFALGIMPYISSSIIMQLMTHASPQLEA
                     LKKEGEAGRR"
     assembly_gap    3223..3322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3323..4000
                     /locus_tag="PLL14_01620"
     CDS             <3323..4000
                     /locus_tag="PLL14_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG02645.1"
                     /translation="VISLLVVLVTAFVVFVERGQRKILVNYAKRQVGNKIYGGQSSHL
                     PLKLNMAGVIPPIFASSIILFPATIAGWFGAGESMRWLKDIAGALSPGQPIYVMLYAA
                     AIIFFCFFYTALVFNSRETADNLKRSGAFVPGIRPGDQTARYIDKILMRLTLVGAGYI
                     TIVCLLPEFLILKWNVPFYFGGTSLLIIVVVTMDFMTQVQAYAMSHQYESLLKKANFK
                     GSGLPVK"
     gene            4002..4220
                     /gene="infA"
                     /locus_tag="PLL14_01625"
     CDS             4002..4220
                     /gene="infA"
                     /locus_tag="PLL14_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG02646.1"
                     /translation="MAKEDLIEMQGEVIENLPNATFRVKLENGHIVLGFISGKMRMHY
                     IRILPGDKVTVQLTPYDLSRARIVFRAK"
     gene            4288..4401
                     /gene="rpmJ"
                     /locus_tag="PLL14_01630"
     CDS             4288..4401
                     /gene="rpmJ"
                     /locus_tag="PLL14_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG02647.1"
                     /translation="MKVLASVKRICRKCKIIRRHGIVRVICTDRRHKQRQG"
     gene            4481..4843
                     /gene="rpsM"
                     /locus_tag="PLL14_01635"
     CDS             4481..4843
                     /gene="rpsM"
                     /locus_tag="PLL14_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG02648.1"
                     /translation="MARIAGVNLPNHQHAEIALTAIYGIGRPRAQKICDAAGVQRSTK
                     MKDLTEAELERLRDQIGKFSVEGDLRREVTMSIKRLMDLGCYRGLRHRKGLPSRGQRT
                     RTNARTRKGPRKPIAGKK"
     gene            4864..5259
                     /gene="rpsK"
                     /locus_tag="PLL14_01640"
     CDS             4864..5259
                     /gene="rpsK"
                     /locus_tag="PLL14_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG02649.1"
                     /translation="MAKTATKVRKKVKIRKNVAEGVAHIHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGRVAVECGVKNLEVRIKGPGPGRESSVRAL
                     NALGMKITAITDVTPIPHNGCRPPKRRRI"
     gene            5272..5901
                     /gene="rpsD"
                     /locus_tag="PLL14_01645"
     CDS             5272..5901
                     /gene="rpsD"
                     /locus_tag="PLL14_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG02650.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFTDKCAIERRSYAPGQHG
                     QKSGQRLSDYGVHLREKQKIRRIYGVLEGQFRKVYKEADRRKGVTGEVLLQLLEARLD
                     SVCYRMGFAASRSESRQVVRHNGVLVNGRRVNIPSYQVRPGDVVEVCDKAKVHLRIKA
                     ALAAAEARGFPEWVEVDAKAAKGTYKAHPERSELPPTINESLVIELYSK"
     gene            5944..6918
                     /gene="rpoA"
                     /locus_tag="PLL14_01650"
     CDS             5944..6918
                     /gene="rpoA"
                     /locus_tag="PLL14_01650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG02651.1"
                     /translation="MQSSGLFKPRIIDVQSLSPVHARVVMEPFERGFGHTLGNALRRI
                     LLSSMPGYAPTEVSIEGVLHEYSTIDGVQEDVVDILLNLKGVVFKLHNRDEVVLQLRK
                     EGKGVVRAADIEVSHDVEIINPEHVIAQLSAGGKLAMELKVEKSRGYVAGNLRQLSDG
                     SKNIGKLVLDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGAIEPEEAIRTAAR
                     ILMEQLSVFADLAGTAMPVEYQKTVQVDPLLLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLENWPPAGLDK"
     gene            6949..7335
                     /gene="rplQ"
                     /locus_tag="PLL14_01655"
     CDS             6949..7335
                     /gene="rplQ"
                     /locus_tag="PLL14_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG02652.1"
                     /translation="MRHRLGLRKLNRTSAHRSAMLRNITVSLLREELIKTTLPKAKEL
                     RRVIEPIITLGKKPSLANRRLAFDRLRDRDMVVKVFDELGPRYATRNGGYLRILKCGF
                     RDGDNAPMAYVELLDRPEAATESADK"
BASE COUNT         1595 a         2128 c         2415 g         1392 t
ORIGIN      
        1 gaagaggcgc gtgccttgct tggcgcgttt aaatttccct tcaggaactg agacgaaatg
       61 gcgaaactcg cagtgatcaa ccgcggtgaa aagcggcgca agacagttga aaaatatgcc
      121 aaaaagcgtt ctgagctgat ggccatcgtc gctgaccaga gccggtccat cgaagagcgc
      181 tatgaagcgc gcctgaaggt gcaggcgctg ccgcgcaatg ccagtccggt aaggctgcgc
      241 aaccgctgcg ctctgacggg tcgcccgcgg ggcgtttacc ggaagttcgg tttgggtcgc
      301 ggcaagctgc gcgagttcgt catgcaaggc gaagtcccgg gaatgatcaa ggctagctgg
      361 taggcaggag aaaacagaat gagtatgagc gatccgatca gcgacatgct gacccgcatc
      421 cgaaatgccc agatggcaag caaggcctcc gtcgccatgc cgtcttcgaa ggtcaagatt
      481 gccatcgccc aggtgctgaa ggacgaggga tacgtagagg acttcgccgt cagcccgaac
      541 gaaggcaagc cgattcttga gattggcctg aagtactatg cgggccgccc ggtcatcgag
      601 cgtatcgacc gcgtgtcgcg cccaggcctg cgtatctatc ggggcgcagg cgatattccg
      661 caggtcatga atggccttgg ggtggcgatc gtgtcgactc cgaagggcgt catgactgac
      721 cgcaaggccc gcgccagcca tgtcggcggc gaagtcctgt gcttcgtcgc ataaggggga
      781 gctatgtccc gcgttgctaa gaaaccaatt gttgtcccgc agggtgtctc cgtcaatctt
      841 tcggcagggc atatttccgt gcaaggaccg ctcggcgtgc tcgactttgc tgtgcattct
      901 gcggtgagcg tcgaacagga aggtgacagc ctgctgtgca agccggtcgc gggtgtggcc
      961 caggcagatg cgctttccgg gaccgtcagg gcggttgttt cgaatatggt taccggtgtc
     1021 agcaagggtt ttgagcgcaa gctgagtctg gtgggtgtgg gctaccgcgc gcaggcgcag
     1081 ggcgatacgc tcaacctgac ccttgggttc tcgcatccgg tggcgtacag gctgcccagc
     1141 ggcatcactg ctgcgacacc ggcgcagacc gagatcgtga tcaagggagt tgaccggcag
     1201 ctggttggcc aggtggcggc tgaagtccgc gcctatcgca agcctgagcc ctacaagggc
     1261 aagggcgtgc gctatgtcga cgaagttgtc gcgatcaagg aaacgaagaa gaaaaagtaa
     1321 gggtgccgag atgattgaca agaaacaggc gcgtttgcgc agagcccgca aaacccgggc
     1381 caagattgcc gaactgaaat ccgtgcgctt gtcggtgcac cgcagcaact gtcacatcta
     1441 cgcacaggtg atttccgcgt gtggatcaaa ggtgctggct gcggcttcat cgcttgagcc
     1501 ggaagttcgc aagatgcttc ccaacggcgg tgacatcgcc gcggcaaaga cgattggagt
     1561 gctgattgcg gaacgggcca ggagtgccgg catcgaacag gtcgcgttcg accgcgccgg
     1621 cttccgctac cacggacgga tcaaggcgct ggcggatgcg gcgcgtgagg ccggtctcaa
     1681 gttctgatcg cagcgcaaag atttggagta agccatggcg aagccacaaa gtagaaaacc
     1741 gcagcagtcc gagaagcctg acgacggcat gcgcgagaag atgatttcaa tcaaccgcgt
     1801 cacgaaggtg gtgaagggcg gtcgcatcct gggatttgca gcgctgacgg tcgtcggcga
     1861 cggcgacggg cgtgtcggga tgggcaaggg caagtcgcgc gaggtacctg tcgcggtgca
     1921 gaaggcaatg gaagaggccc gccgcaatct gatcaaggtg agcctaaaga acgggaccct
     1981 gcagcatacc gtcgtcggtc atcatggcgc ctcgcgcgtg atgatgcagc cggcgcccga
     2041 aggtaccggg atcatcgccg gcggcgccat gcgcgccgtc ttcgacgtca tcgggatggc
     2101 caacgtggtc gccaaggcgc acggttcgac caacccctac aatattgtcc gggcaacgat
     2161 caacggattg cggtcgatga ccacgccggc cgaagtcgcc gccaagcggg gcaagtccgt
     2221 cgccgagata ctggggtaag aaatgaccga caaaaaagtg aaaatcacgc tggtcagaag
     2281 cctgatcggc accaaggagt cgcatcgtgc gacggtacgc ggcctgggtc tgcggcgcat
     2341 gcacagcagt tcgctcctcg aagacacccc ggcggtacgc gggatgatca acaaggtgtc
     2401 gtacctcttg aagtgtgagg gctgagatgg aactgaatag cgtcaagggt gccgctggcg
     2461 cccggccgga ccggaagcgc gtcggtcgcg gcatcggatc gggtcttggc aagacctgtg
     2521 gacgtggcca caagggacag aagtcgcgtg ccggaggctt ccacaaggtt ggcttcgaag
     2581 gcggtcaaat gccgctgcaa cgccgccttc cgaagcgcgg tttcaagtcg atgaccggag
     2641 agcgcaactt cgaggtccgc ctgtccgaaa tcgcccgcct gccggtcgat gagatcgacc
     2701 tggcgacgct gatccaggcc agcctggtgt cgcagcatgc gctgtcggcg aaggtggtgc
     2761 tttcgggcga gatccatcgc aaggtcacgc tcaagggtgt tgcagcgacg gccggagcac
     2821 gcgcagccat cgaagccgcc ggcggcagcg tttcggcata acgagcaggc aaaggtgatc
     2881 ttttttggta actagttcga gcccgatcag caaaggtgga aagttcggcg atctgaagcg
     2941 ccggctgtgg ttcctgctcg gtgcgctggt agtttatcgc atcggctcgc acatcccggt
     3001 tcccgggata gatgcgcagg tgttgagcga actgttcaac aagcagcaag gcggcatcct
     3061 gggcatgttc aacatgttct cgggcggtgc gctgtcgcgc ttcaccattt tcgcgctcgg
     3121 gatcatgccg tacatttcgt cgtcgatcat catgcaactg atgacccacg ccagccccca
     3181 actggaggcc ctgaagaagg agggggaggc cggacgccgc aannnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nngtgatcag cctgctggtg gtgctggtga cggccttcgt
     3361 ggtgttcgtc gagcgcggcc agaggaagat cctggtgaac tatgccaagc gccaggtggg
     3421 caacaagata tacggtgggc agagttcgca tctgccattg aaactgaaca tggccggggt
     3481 cattccgccg attttcgctt cgtcgatcat tctgttcccg gcgacgatcg ccggctggtt
     3541 cggcgctggc gagtcgatgc gctggttgaa ggacatcgcc ggtgccctgt cgccggggca
     3601 accgatctat gtcatgttgt acgctgcggc gatcatcttt ttctgctttt tctacaccgc
     3661 gctggtgttc aattccagag aaaccgccga caacctgaaa cgcagcggcg cgttcgttcc
     3721 gggaatccgt ccgggggacc agacggcacg ttatatcgac aagatcctga tgcgcctgac
     3781 cctggtcggc gccgggtata tcaccatcgt ttgtctgctg ccggagttcc tgatcctcaa
     3841 gtggaacgtg cccttttact ttggcggcac ttcactgctg atcattgtgg tggtgacgat
     3901 ggatttcatg acccaggtac aggcgtatgc catgtcgcac cagtatgaga gcctgttgaa
     3961 aaaggctaac ttcaaaggct ccggtttacc ggtgaaatag gatggcaaag gaagatttga
     4021 ttgaaatgca gggcgaggtc atcgagaacc tccccaacgc caccttccgg gtcaaactgg
     4081 aaaacggcca catcgtcctg ggtttcatat ccggcaagat gcggatgcac tacatccgga
     4141 tcctgccggg cgacaaggtg acagtgcagc ttacgcccta tgatctgagt cgcgcgcgaa
     4201 tcgtcttccg cgccaagtga agaatctcta cgcaacaacc cggcgaaagg ctggctgcgc
     4261 ctcgtgccgg ggaaacagga gtgaatcatg aaagtgctgg cctctgtgaa acgcatttgc
     4321 cgcaagtgca agattattcg gcgccacggc atcgtgcgcg tgatctgtac ggatcggcgt
     4381 cacaaacagc gtcaaggttg atcgctgcga cccacaaatc tgtggattgt cagacggttg
     4441 aaggtataac tgtaagtttc gattattttg gggtgaatcg atggcccgca tcgcaggcgt
     4501 aaacctcccg aaccaccagc atgccgagat cgcgctaacc gccatctacg gtataggacg
     4561 tccgcgcgca cagaaaatct gtgatgcagc tggggttcaa cgttcaacaa aaatgaagga
     4621 cctcaccgag gccgaactgg agcgcctgcg cgatcagatc ggcaagttca gcgtcgaggg
     4681 cgatctgcgt cgcgaagtca cgatgagcat caagcgattg atggatctgg gctgctatcg
     4741 agggcttcgg caccgcaagg gcctgccgtc gcgtggtcag cggacgcgta ccaacgcccg
     4801 tactcgcaag ggtccgcgga agccgatcgc cggcaagaaa tgattaggaa ctaggacctg
     4861 actatggcca agaccgctac aaaagttcgc aagaaagtca agatcagaaa gaacgtggcc
     4921 gagggtgtcg cacacatcca cgcctcgttc aacaatacga tcatcaccat taccgatcgc
     4981 caggggaatg cgctgtcgtg ggcaacctcg ggcggcgccg gcttcaaggg ctcgcgcaag
     5041 agcacgccgt ttgctgcgca ggtggccgct gaagccgccg gcagggtggc cgtggagtgc
     5101 ggcgtcaaga atctcgaagt gcgcatcaag ggtccaggcc ccggacgtga atcctcggtg
     5161 cgcgcgctca atgcgctcgg catgaagatc acggcgatta ccgatgtgac gccgatcccg
     5221 cacaatggct gccgtccgcc caaaaggcgt cggatttaag gagaaggaaa agtggctcgc
     5281 aatctcgatc cgaagtgccg tcagtgccgc cgtgaaggcg aaaagttgtt cctcaagggc
     5341 gaaaagtgct ttaccgacaa gtgcgcgatc gaacgtcgct cgtacgcacc cggtcagcat
     5401 ggccagaagt ccgggcagcg tttgtccgac tatggcgttc acctgcggga gaagcagaag
     5461 atccggcgga tctatggcgt tctcgaaggc cagttccgca aggtgtacaa ggaagccgac
     5521 cggcgcaagg gtgtgactgg cgaagtgctg ctgcagttgc tcgaagcgcg cctcgacagt
     5581 gtctgttacc gcatgggctt cgccgcctcg cgctcggagt cgcgtcaggt cgttcgccac
     5641 aatggcgtgc tggtcaacgg tcgccgggtg aacatcccgt cgtatcaggt ccgtccgggt
     5701 gacgtcgtcg aagtctgcga caaggcaaag gtccacctgc gtatcaaggc cgcgcttgcc
     5761 gccgctgaag ctcggggttt tccggagtgg gtcgaggtgg atgcaaaggc tgcgaagggc
     5821 acctacaaag cgcacccgga acgtagcgaa ctgccgccga cgatcaacga gagtcttgtc
     5881 atcgaactct actcgaagta gtcgtagcga cgaggcttga cggcatcagg caaaggacag
     5941 tacatgcaaa gcagtggact atttaaaccg cgcatcatcg atgtgcagag cttgtcgccg
     6001 gtgcacgccc gggtggtcat ggaaccgttc gaacgcggct tcgggcacac gctcggcaac
     6061 gcgctgcgac ggatcctgct ctcgtcgatg ccgggttacg caccgaccga ggtcagcatc
     6121 gaaggcgtgc tgcatgaata ttcgacgatc gatggcgtcc aggaggatgt cgtcgacatc
     6181 ctcctgaatc tcaaaggggt ggttttcaaa ctgcacaacc gcgatgaggt cgtcctgcaa
     6241 ctccgcaagg aaggcaaggg tgtcgtccgg gccgcggata tcgaggtttc gcatgacgtc
     6301 gagatcatca atcccgagca tgtgatcgcg cagctttcgg ccggcggcaa actggcgatg
     6361 gaactgaagg tcgagaagtc gcgtggctac gtggccggca acctgcgcca gttgagtgac
     6421 ggcagcaaga atatcggcaa gctggtcctc gacgcctcgt tcagcccggt gcggcgggtc
     6481 agttatgcgg tcgagagcgc gcgtgtcgaa cagcggaccg atctcgacaa gctgatcatg
     6541 gacatcgaaa ccaacggcgc gatcgagcct gaagaggcga tccgcaccgc cgcccggatt
     6601 ctgatggaac agctttcggt gttcgccgat ctcgccggca cggccatgcc ggtcgaatac
     6661 cagaagacgg tgcaggtcga cccgttgctg ctgcgtccgg tggacgacct ggaattgacg
     6721 gtgcgctcgg cgaactgcct gaaagcggag aacatctact acatcggtga cctgatccag
     6781 cgcaccgaga acgagttgct caagaccccc aatctgggtc gcaagtcgct gaatgaaatc
     6841 aaggaagtgc tggccgcgcg cggcctcaca ctgggcatga aactcgagaa ctggccgccc
     6901 gccggactcg acaagtgatc cgccggacaa gaacaggaag gaattgatat gcgtcaccgt
     6961 ttgggtcttc gcaaactgaa tcgtaccagc gcccatcgct cggcgatgtt gcgcaacatt
     7021 accgtctcgc tgctgcgcga ggagttgatc aagacgacgc tgccaaaagc gaaggaactt
     7081 cggcgcgtca tcgaaccgat catcacgctc ggcaagaagc cgagcctggc caaccgccgc
     7141 ctcgccttcg accgcctgcg cgaccgcgac atggtggtca aggtgttcga cgaactcgga
     7201 ccacgttacg cgacccgtaa tggcggctat ctgcggattc tgaagtgtgg tttccgcgac
     7261 ggagacaatg cgccgatggc gtatgtcgaa ttgctcgacc gtccggaagc cgcgacggag
     7321 tctgctgaca agtagaaggc acgcatcgcc gatccgcaaa gccaggccag gcctggcttt
     7381 tttgtgcctg atcgaaaggc gggtgagacc ttgccggccc cgcaaagccg gcagtttctc
     7441 gctcgaatca gcaccccggc gcaatgttct tcctccgcgt cccgcccgcc tttggccagt
     7501 acggaacgcg caggcagatc ctcgacgact ggccggccgt cttttccccg gtgcgtgcgc
     7561 ggctgttcac ccctgcgcgg cgatcggcat ctggcggccg ttgccgaagg cgcgaccgcg
     7621 cagacgaatg
//
LOCUS       DAOSIT010000033         7501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9028_length_7501_cov_5.932984, whole genome shotgun sequence.
ACCESSION   DAOSIT010000033 DAOSIT010000000
VERSION     DAOSIT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9028_length_7501_cov_5.932984"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..341
                     /locus_tag="PLL14_01660"
     CDS             156..341
                     /locus_tag="PLL14_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02653.1"
                     /translation="MNTKELAIRTLEELPEDATWEDVQERINFLIGVRKGLRELDEGN
                     GIPHDRVKEEFAQWLTC"
     gene            326..634
                     /locus_tag="PLL14_01665"
     CDS             326..634
                     /locus_tag="PLL14_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG02654.1"
                     /translation="MAYLLIWSPSARFDLREISAYIAEADPIAAAKFIRGVFNTVERL
                     SRFPESGRIVPDFGDPNIREVIRRPCRIVYRIRHSSEVVEIIRVWHAARGVPELPWND
                     "
     gene            841..4122
                     /gene="drmD"
                     /locus_tag="PLL14_01670"
     CDS             841..4122
                     /gene="drmD"
                     /locus_tag="PLL14_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HOG02655.1"
                     /translation="MASVTQQKQIPEPGQVVRVRTRTYLVEAVAPSPGGHLVTMACLD
                     DDAQGQALKVVWEIELDTEILDEEAWRSIGAKGFDSPRYFGAYMHTLRWNCVTATNPR
                     LFQSPFRAGIRIDAYQLEPLQKALRLPRVNLFIADDVGLGKTIEAGLIASELLLRRRI
                     SEIVVACPPSMLVQWQDEMETRFGLTFEILDRDYVEKVRRERGYGVNPWTTFPRFLIS
                     QRLLIDEAYAAPLRDWLENLRSGSLLILDEAHHAAPASGAKFAIDSRITRAVRDLAPR
                     FEHRLFLSATPHNGHSNSFSALLEILDSNRFARGVKVLKSNLDDVMVRRLKEDIREIE
                     GGFPERKVEQIDIQGLPDDAPELVLSRLLNAYREVRQRRVEGQSKRRQAEAALLISGL
                     QQRLLSSVDAFARTLAVHRRTMERLWQQTGGGASATASTKTARSSEKGLMLLGGSLGN
                     DDERAELTEEELEALEDAQFEVSTAATAGDSACADVAREKELIRQMTEIADQARGRPD
                     ARVCHLLKWIQEHMCPGIRLPDQPRPASGAPWNDRRLLIFTQYDDTKRYLVKMLQAAI
                     AGTELAEYRIEIFHGPTPLKKREQIKRAFNAPPDQHPVRILIATDAAREGINLQAHCH
                     DLFHFDVPWNPSRLEQRNGRIDRKLQPSPKVFCRYFVYTQRPEDQVLRALVRKTSTIQ
                     DELGSLSQVLEARLAETLRIGIRHESAGDLARDIEDADIDPERKAVAEEELDAVRQRR
                     DQLREEIKTLNRRVEESRKWIGLDNDALRDALSCALEMLGAEPLQEDGTPPKGHPVRY
                     RFPNLESRRGGDPSWAPTLDTLRKPPDEGEAGFQWRQNAPIRPVTFDPPPGIDDSTVQ
                     LHLQHRVVQRLLGRFRAQGFVHHDLSRACLTQSQDRIPRVILLGRLSLYGSGATRLHE
                     EILTVTARWLPPDQRSGALAPYARETEARTLELLEAALHPKAQKELPKHVQTTLLASL
                     PRDITELLPHLQSRGESARSDAEAALAERGRAESSAMRRILEDQRRRILAELGTPMIQ
                     LTLPGMDDAERRQRESNRRYWQRWIDNVQDDLEREPARILNFYQVKSCRIEPIGIAYL
                     YPASN"
     gene            5687..>7501
                     /locus_tag="PLL14_01675"
     CDS             5687..>7501
                     /locus_tag="PLL14_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG02656.1"
                     /translation="MVLVPTDERRRTGSHYTPRALTGPIVRKTLEPVLRQLGDRPTPD
                     QILNLKVCDPAMGSGAFLVEACRQLASALVEAWAAHGWKPYIPPDTTEELHAQRLVAQ
                     RCLYGVDRNPMAVDLAKLSLWLATLARDLPFTFLDHALRTGDSLVGLTRLQIMGFHWA
                     PEQQPAFLEARIRECLEKVTRTRRQILDAGDNMPPGNKRQILGVADEQLDTVRLLGDA
                     VVSAFFHGGKKREREERLVQLFVKAEDRYIRNDFAALLDLKAAARELRTGAHPVTPFH
                     WEAEFPEVFAPDRGGFDAIVGNPPFAGKNTLINGNRAGYLDWLQTIHEEAHGNSDLVA
                     HFFRRAFGLLRPDGAFGLIATNTIGQGDTRHTGLRWICTHGGTIYAATKRYKWPGLAA
                     VVVSVVHVAKGEPEGPFELDGRAVPVITAYLFHAGGHDTPATLHANAGKSYIGSYVLG
                     MGFTFDDTDKKGVANPISLMHELIAKDPRNAERIFPYLGGEEVNTSPTHAHHRYVINF
                     EDFPLRRAELQGTWKGADEQKRTAWLRTGIVPLDYPDPVAADWPDLLAIVEAKVKPQR
                     NKDKREVRRKYWWRFGETTPALFAAIAGLERVLTISRVS"
BASE COUNT         1414 a         2508 c         2264 g         1315 t
ORIGIN      
        1 caaaggacat ggccgtaaag ccgacgcctc gcgtggccgg tgcccggtca aataaaatcg
       61 ctgctcggcc ttttcgccgc agaaggggca aaggccgatt gctattgtgg ttggatcaga
      121 ttgcttgtct tggaactgtg tgttaaagtg aaaccatgaa cacgaaagaa ctcgccattc
      181 gtacacttga ggagttgccc gaagacgcga cttgggaaga tgtgcaggag cgtatcaact
      241 tcctgatcgg cgtccgtaag ggactccggg agttggatga aggcaatggt attcctcatg
      301 accgcgtcaa ggaggagttc gcccaatggc ttacctgttg atttggtctc cctccgcccg
      361 gttcgacttg cgcgagatca gtgcctacat tgccgaagca gaccccatag ccgcagccaa
      421 attcatccgt ggcgttttta acaccgtgga gcgtctgtcg cgctttcccg aatcaggacg
      481 aatcgttccc gatttcggcg acccaaacat ccgagaagtg attcgccgac cttgccggat
      541 tgtatatcgt atccgccact caagcgaagt tgtggagatc atccgcgtat ggcacgccgc
      601 aaggggtgtt cccgagttgc catggaacga ctaagggaca ggtatttagg acgtttaatc
      661 ctgcttaata tcgtcagcga agcagtaaag aagtcgttat ttggagggcg atccaatatt
      721 ctgctctggc cgatcgcgac agctatactt ttgccgttgt gaacattgcc caagtttgga
      781 taatatggcg tcacacgcgc gaccgagtag cggtgaaatc atccagattt ggacagaagc
      841 atggcgtcag taacacagca gaaacagatc cccgagcccg gccaggtggt tcgtgtgcgc
      901 acgcggacct atctggtgga ggcggtcgcc ccgagccccg gcggacatct ggtcaccatg
      961 gcgtgcctgg atgacgatgc gcagggacag gcgctcaagg tggtctggga aatcgagctg
     1021 gatactgaga tccttgacga ggaggcgtgg aggtcgatcg gcgcgaaggg gttcgacagc
     1081 ccgcgttatt tcggcgccta catgcacacg ctccgctgga actgtgtgac cgccacgaac
     1141 cccagattgt tccagtcgcc gttccgtgcc ggtatccgta ttgatgcgta tcagcttgaa
     1201 ccgctccaaa aggcgctgcg cctgccgagg gtcaacctgt tcatcgctga cgatgtcggt
     1261 ctcggcaaga ccatcgaggc cgggcttatc gcctcggaac tgctgctccg ccgacgcatc
     1321 agcgaaatcg tggtcgcctg tccgccttcg atgctcgtgc agtggcagga tgagatggaa
     1381 acgcggttcg ggctcacctt tgagattctt gatcgagact atgttgagaa ggtgcgccgg
     1441 gaacgcggct acggggtcaa tccatggaca acctttcccc gctttctcat ctcccagcgc
     1501 ctgttgatcg atgaggccta cgccgcgcct ttgcgcgact ggttggagaa cctgcgctct
     1561 ggctctttgc tcattctgga tgaggctcac cacgccgccc cggccagcgg tgccaaattt
     1621 gccatcgact cgcgcatcac ccgcgcggtc cgtgacctcg cgccccggtt cgagcatcgc
     1681 ctgttcttat cggcgacacc gcacaacggc cactcgaaca gctttagtgc gctgctggag
     1741 atccttgact ccaaccgctt cgcacggggt gtcaaggtgc tgaagagcaa tctggatgat
     1801 gtcatggtgc ggcgcttgaa ggaggacatc cgcgagatcg agggcggctt ccccgaacgg
     1861 aaggtggagc agatcgatat ccaaggtctc cccgatgacg ctcccgagct ggttctgtcg
     1921 cggctgctga acgcgtaccg tgaagtccgg cagcgccggg tggaggggca gagcaagcgc
     1981 aggcaggctg aagcggcgct gctgatctcc ggcttgcagc aacgcctcct gtcgtcggtc
     2041 gatgccttcg cccgtacctt ggccgtccac cggcggacca tggagcggct ctggcaacag
     2101 acgggaggcg gcgcttcagc caccgcgtcg acgaaaacgg ctcggagttc agagaaaggc
     2161 ctgatgctgc tgggcggcag tctcggcaac gatgacgagc gggccgaact gaccgaagag
     2221 gagcttgagg cgcttgagga cgcccagttc gaggtttcga ccgcagccac ggcgggcgac
     2281 agcgcatgcg ccgatgtcgc ccgcgagaag gagctgatcc ggcagatgac ggagatcgcc
     2341 gatcaagccc gcggacgccc cgacgcgcgt gtctgccatc tgctcaagtg gattcaggaa
     2401 cacatgtgcc ccggcatacg tctgcccgac caaccccggc ccgcttccgg cgcgccatgg
     2461 aatgaccggc ggctcctgat tttcacgcaa tacgatgata ccaagcgtta tctggtgaag
     2521 atgctgcagg cggccatcgc cggtaccgaa ctcgccgaat accggatcga gattttccat
     2581 gggccaacgc cactcaaaaa gcgcgaacag atcaaacgcg ccttcaacgc cccgccggat
     2641 cagcatcccg tgcgcatcct gatcgcgacg gacgcggcgc gggagggcat caaccttcag
     2701 gcccactgcc acgatctctt ccatttcgac gtgccgtgga atcccagccg cctcgagcag
     2761 cgcaacggtc gcatcgaccg caaactgcag ccgtcgccga aggtgttctg ccgctacttc
     2821 gtctatacgc agcgccccga agaccaagtg cttcgtgcgc tcgtccgcaa aacttccacc
     2881 atccaagatg aactgggcag tctgtcccaa gtgctcgaag cacggctggc ggaaaccttg
     2941 cgcatcggca tccgtcacga gtcggcgggc gatctggcca gggacatcga ggacgcggat
     3001 atcgaccccg agaggaaggc ggtggccgag gaggagctgg atgccgtgcg ccagcgccgg
     3061 gatcagttgc gcgaggagat caaaacgctc aaccggcgcg tggaagagtc gcgcaaatgg
     3121 atcggcctgg ataacgatgc cctccgtgat gccctctcct gcgctctgga aatgctgggc
     3181 gccgagccct tgcaggagga cggcacgccc ccaaaaggac atccggttcg ctatcgcttc
     3241 ccgaacctcg agtcccgccg gggcggcgac ccgtcatggg cgccgaccct cgatacgctg
     3301 cgcaagccgc cggatgaagg cgaggccgga ttccaatggc ggcagaacgc gcccatccgc
     3361 ccggtgacct ttgatcctcc ccccggcata gacgacagca cggtgcaact tcaccttcag
     3421 caccgtgtcg tgcaacgcct tctcggcagg ttccgcgctc agggcttcgt tcaccacgac
     3481 ctctcccgcg cctgcctgac gcagagccaa gaccggattc cgcgtgtgat cctgctcggt
     3541 cgcctctcgc tttacggctc aggcgcgacc cgtctgcatg aggagatcct cacggtcacc
     3601 gcccgctggc tcccgcccga tcaacgcagc ggcgccctcg cgccctacgc cagagagacc
     3661 gaagcccgga cgctcgaatt gctggaagcg gcgctccacc ccaaggcgca gaaggagctg
     3721 ccaaaacatg tgcagacgac cctgctggcc tcgctgccgc gcgacatcac cgagttgctg
     3781 ccgcacctcc aatcgcgcgg cgagagcgcc cggagcgatg cggaggccgc gctggcggag
     3841 cgcggccgcg ccgagtccag cgccatgcgc cgtatcctcg aggatcagcg caggcgcatc
     3901 ctggcagagc tgggaacccc gatgatccag ctcacgctgc ccggcatgga tgacgccgaa
     3961 cgccgccagc gcgagtcgaa ccggcgctac tggcaacgct ggatcgacaa cgtccaagac
     4021 gatctggaac gcgaaccggc acgcattctc aacttctatc aggtcaagtc ctgccgtatc
     4081 gagccgatcg gcatcgccta cctctatccc gcgagcaact gacaccatgg caaccgatcc
     4141 tgcaattcga gatcaccaga cctggctggg ctacctgcag ccagagggcc tcgtggtgtc
     4201 ccccgccgca ctggtagacg cccaagtcgc cgtcaacacc aacgccctgc cgctgcagca
     4261 gcgctttctg caattcatcg aggagatcga gtatcaggac ggcgagatca tcgccatcca
     4321 ggatctgggc gtgttcctgc gcgagttcct tggctggccc gaggaacgcc tggtcggcaa
     4381 gggctgtgcc gagccgaccc ccgaaccgct caaggcaccg ctccgcgagt tcggcgaaac
     4441 gcttgagccc gacctggcct tcatcgaccg gcaacctgcc gacgacgccg tcccgtggct
     4501 cctgctggtg caggatttgc cgctgggcac cgatctggat gtgccggtcg aatcggccga
     4561 ggccggatgg gccgcgtcac atacccggcg cttcgaacga ctgctgcggg cggtcaaggt
     4621 gcccatcggc ctgctttcca accgcaccca cttgcgtctg gtctacgccc cgcacaacga
     4681 gaacgcggga acgctgacct tccctgtcgc ggccatgtcg gaagtggcgg gccgtccgat
     4741 cctggcggcg ctcgaaatgc tgctgtcgcg ctaccgcctg ctggccgccc cctcggaagc
     4801 gcgtctgccg gcgctgctcc agcgcagccg ggaataccaa tcccgcgtct caaccgcctt
     4861 ggccaagcag gtgctcaact cgctctatga gctgctgcgc ggctttcagg ccgccgatga
     4921 acacagccgc ggcgacctgc tgcaccaggt gctggccgaa catccggacg cggtctacta
     4981 cggcctgctc acggtgctca tgcgcctggt cttcctgctc tacgccgaag atcgcggcct
     5041 gatgcccggc ggcgatctct atgtgcgcaa ctacgccatc cacggtctct tcgaacgcct
     5101 gcgcaccgat gcccggcagt tcccggacac catggatcac cgctacggag cctgggcgca
     5161 gctcgtggcg ctgttccgcg ccgtccacaa cggctgcacg cacaagctgc tcgagatgcc
     5221 ccgccgcgaa ggccatctct tcgaccccga ccgcttcccc ttccttgaag gacgcacaac
     5281 ggcagaggct cgcttgccgc tggtcgcgga cggcgtcgtc taccgggtgc tggaaaacct
     5341 gctcgtgctg gacggcgaac ggctgagtta ccgaacgctc gacgtcgaac agatcggcag
     5401 cgtctacgag accatgatgg ggttcgaact caagctgacg cagggacaga ccatcgccct
     5461 ccagcccggc aagacccacg gcgcgccgat cccggtcaac ctcgaagcgc tgcttgcgac
     5521 cccacccgaa cagcgcgcca aatggattct cgaacgtacc gactacaaac tcaccgccac
     5581 gatgaccagc gccgtcaagg ctgccgactc gctcgacacc ctgctggcgg cgctcgaacc
     5641 gcgcatcgcc cgcgaggcca cgccgcaccc cgtaccgccg ggggccatgg tgctggtgcc
     5701 gaccgacgag cggcggcgca ccggctccca ctacaccccg cgcgcgctga ccggccccat
     5761 cgtccgcaag accctcgagc ccgtcctgcg gcagcttggc gaccgcccga cgcccgatca
     5821 gatactgaac ctgaaagtgt gcgatcccgc gatgggctcc ggcgcctttc tggtcgaggc
     5881 gtgccgccag ttggccagtg cgctggtcga ggcgtgggcc gcccacggtt ggaagcccta
     5941 catcccgccc gacaccaccg aggaactcca tgcccagcgc ctcgtcgccc agcgctgcct
     6001 gtacggtgtg gaccgcaacc ccatggcggt ggatctggcc aagctctcgc tctggttggc
     6061 cacattggcg cgtgatctgc ccttcacctt cctcgatcac gcgctacgca ccggcgactc
     6121 ccttgtcgga ctgacgcgcc tgcagatcat gggctttcac tgggcgcccg agcaacagcc
     6181 cgcctttctt gaggcgcgta ttcgggaatg cttggaaaag gtgacgcgca cccggcggca
     6241 gattctcgat gcgggcgaca acatgccgcc cggcaacaag cgccagatac tcggtgtggc
     6301 ggatgaacag ctcgatacgg tgcgcctgct cggtgacgcg gtggtttccg cctttttcca
     6361 cggcggcaag aagagagagc gcgaggaacg cctggtccag ctcttcgtca aggccgagga
     6421 tcgctacatc cgtaacgact tcgcggcgct gctagacctc aaagccgccg cccgcgaact
     6481 ccgcacgggc gcgcatccgg tcaccccctt ccattgggag gccgagttcc ccgaggtctt
     6541 cgcgccggat cggggcggct tcgacgccat tgtcggcaat ccgccgttcg cgggtaaaaa
     6601 caccctcatc aacggtaacc gcgcgggcta tctcgattgg ctgcagacga tccacgaaga
     6661 agcgcacggc aactccgact tggtggccca cttcttccgt cgcgccttcg ggctgttgcg
     6721 cccggacggc gcgttcggcc tgatcgccac caacaccatc ggtcagggcg acacgcgcca
     6781 taccggcctg cgctggatct gcacccacgg cggcaccatc tacgccgcca ccaagcgcta
     6841 caagtggccc ggcttggccg ccgtggtcgt cagtgtggtg catgtagcca aaggcgagcc
     6901 agaggggccg tttgagttgg acggtcgtgc ggtccccgtc atcaccgcct acctctttca
     6961 cgccgggggg cacgataccc cggccacgct gcacgccaac gccggcaaga gctacatcgg
     7021 tagctatgtc cttggcatgg gctttacctt tgacgacacc gacaagaaag gcgtcgccaa
     7081 tccgatcagc ctgatgcacg aactgatcgc caaagatccc cgcaacgccg agcgcatctt
     7141 cccctacctt ggcggcgagg aggtcaacac cagtccgacc cacgctcacc accggtatgt
     7201 gatcaacttc gaggatttcc ccctgcgccg cgccgagttg caaggcacat ggaagggtgc
     7261 cgacgagcag aagcgcaccg cttggctgcg taccggcatt gttcctctcg actatcccga
     7321 tcccgtcgcc gccgactggc ccgacctgct tgccattgtc gaggcgaaag tgaaaccgca
     7381 gcgtaataag gacaaacgag aagtccgcag gaaatactgg tggcggtttg gagaaacgac
     7441 tcccgccctt tttgccgcta tcgcagggct ggagcgcgtg ctgacgattt cgcgagtgag
     7501 c
//
LOCUS       DAOSIT010000034         7148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9538_length_7148_cov_9.587904, whole genome shotgun sequence.
ACCESSION   DAOSIT010000034 DAOSIT010000000
VERSION     DAOSIT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_7148_cov_9.587904"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="mtnA"
                     /locus_tag="PLL14_01680"
     CDS             complement(<1..896)
                     /gene="mtnA"
                     /locus_tag="PLL14_01680"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOG02657.1"
                     /translation="MLINGTPTRTIQPVADARAVDIIDQTALPHVYRILRLRTLAEVA
                     HAIRSMQVRGAPLIGVAAAYGVALALTQRADDATLAVAIETLAATRPTAVNLRWALAR
                     LQAVLQTLPPSARADAAWREAATLAEEDVQQCRRIAAHGLQLLRAGIAQKGRLEIMTH
                     CNAGWLATVDYGTALAPVYAAFDAGIDVHVWVSETRPRNQGLLTAWELGRHGVPRTLI
                     ADNAAGLLLQNGRIDAVMVGADRVAANGDVANKIGTYLKALAARAAAVPFYVAAPRST
                     IDFACPNGAAIPIEERDADELRV"
     gene            1057..1614
                     /locus_tag="PLL14_01685"
     CDS             1057..1614
                     /locus_tag="PLL14_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HOG02658.1"
                     /translation="MKLPSRVKALRTPTLLFCALAAGHLAGSLQAAEMPKRKAGLWEI
                     NTRMDGMPAMGAIQQCVDQKTDDLMQQQARKEQPNCSVIDVNNQGNKVSVHSVCKVQG
                     STATTDGVFVGSFDAAYKGDMLTRFNPPLHGTKESKVSLEARWVGPCKAGQKPGDVIM
                     PNMAGSNLKEMMKDPTVQEMMKRQK"
     gene            1891..3567
                     /locus_tag="PLL14_01690"
     CDS             1891..3567
                     /locus_tag="PLL14_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG02659.1"
                     /translation="MSYSAFVQARDFLLAHRTDYETAYRDFQWPQLGEFNWALDYFDV
                     MAAGNDRPALWVVNEDGSEQKMSFSEMSRRSNQVANWLRAQGVKRGDRILMMLGNEVP
                     LWDTMLASIKLGAVLSPATTLLAPDDLRDRLDRGQVRHVIIGHTHAEKFSDLPGDYTR
                     VTVGGAVAGWQQLEQAYEGSSDFQPDGPTQATDPQLLYFTSGTTSKPKLVLHSQQSYP
                     VGHLSTMYWLGLQPGDVHWNISSAGWAKHAWSCFFAPWNAGATVFIYNYARFAAASIL
                     ETLVRCSVTTLCAPPTVWRMLIQEDLSKYPVKVRELIGAGEPLNPEVIDQVKEAWGIT
                     LRDGFGQTETTAQVGNSPGQAVKLGSMGRPMPGYQVRLVDMDGHVSDEGEICLTLSPR
                     PLGLMISYDDDPEKTAEVMRDGFYHTGDTAAIDADGYITYVGRADDVFKASDYRISPF
                     ELESVLIEHEAVAEAAVVPSPDALRLAVPKAFLTLRAGYEPSRELAREIFVFLRSRLS
                     PYKRVRRLEFGELPKTISGKIRRVELRKGEQDKAAGPRGAMEFYEEDFQF"
     gene            complement(3684..5579)
                     /locus_tag="PLL14_01695"
     CDS             complement(3684..5579)
                     /locus_tag="PLL14_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOG02660.1"
                     /translation="MSAFVSSPAAAAMPEDPASPRKTLSAFASEQELAGLFKRWTDEA
                     QRRRDEQRARRDKSSEFVVGQVLPAPAPAAAAPAAVAKEAAGAKNESVTNVQHAGVDE
                     GGIVKVHGEHLVILRRGRLFTIRIGDNQLRPVSAIDAYGRDVDPGGAWYDEMLISDNT
                     IVVIGYSYARGGTEVGVFDISRAGQLAYRATYHLRSNDYYSSRNYASRLIGSKLIVYS
                     PHILSPWGDPYAAFPAMRRWREGAQASDFRRIAPATRIYRSDEALDPFAGAALHSVTV
                     CDLAKPEMDCQSTAVLGPPGRVFYVSPTAVFVWTTAWRGAPQAAVATSAVFRIPLDGS
                     APSALKTMGSPIDQFSFLEGDGMLNVLLRSNGRGDGMWAAEVNAGDLALLRVPLASFS
                     DGRESAPMAAYRRLPKAAGYAIQSRYVGPFLLYGGGTGWHAPQATNRSQVYAVRYAQG
                     EVYELPLPHAVDRLEALGGNALVVGSDGKDLHLTSVRLGSLPMTVGQYTRRDAAQGET
                     RSHGFFYKAESELDGLLGLPIVGGSESGIRQLGTESAALLFLRNRGLLLSELGTLKAR
                     PSASDRNDACRASCVDWYGNSRPLFVRGRVFALMGYEIVEGKVDETRIVETRRVNFAP
                     TVLQVAP"
     assembly_gap    5606..5705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5706..>7148)
                     /locus_tag="PLL14_01700"
     CDS             complement(<5706..>7148)
                     /locus_tag="PLL14_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HOG02661.1"
                     /translation="QPIDLVASRRPFDDPGVERVYYRLEREKETIVDKTHMPYALGQA
                     RMARWRELFLAPDYRVDELPSYALDVAANPFIAFRALPTTARYQFMLDEAQFTIMGFI
                     KGPVCRGQVALNVIEDQFWVFFEANADDAQQQLGDFLAREASLLKLPAELGSDAGIIR
                     PWREYARREASYLQKKSDFLTRFTATNRRPDLNWIWDGNGRNPNAALTVFRHFDSASV
                     VKGLVGQPPKTAWVIGYGLLERIHYLLVAGFDVYGNVGHQLLTRLYMDFMRMEGEFNF
                     IAFLPRDRRMAVRDYWYQGASEEVKEHVYGRLASLDSETGIRFQGDDPQQEFYTLLRK
                     RLGPVLDHRYDLPALGDVGLQNDLAALAELRGPALSWLPELVYLRIDEGERAPRYVTL
                     LRNTGHSNVSSLLREGQELRPEENTLTVAAGFIGAYPNAIYRVQRSEIRVLAQAIREL
                     SSESDYHALADRYVVRRSNPRFWEYSDHLQQ"
BASE COUNT         1319 a         2305 c         2219 g         1205 t
ORIGIN      
        1 acgcgcaatt cgtcggcgtc gcgctcctcg atcgggatgg cagcgccgtt cgggcaggca
       61 aagtcgatcg tcgaacgcgg cgccgcgaca taaaacggta ctgcggccgc gcgtgcggcc
      121 agcgccttga gataggtccc gatcttgttg gcgacgtcgc cgttggcggc aacgcgatcg
      181 gcgccgacca tcaccgcgtc gatgcgaccg ttctgcaaca gcagaccggc cgcgttgtcg
      241 gcgatcagcg tgcggggtac gccgtgccgc ccgagttccc aggcggtcaa caggccctgg
      301 ttgcgcgggc gggtctcgga aacccagaca tgcacgtcaa ttcccgcgtc gaaagcggca
      361 tagaccggcg ccagcgcggt gccgtagtcg accgtcgcca gccaaccggc gttgcaatgg
      421 gtcatgattt cgagccttcc cttctgcgcg atgccggcac gcagcaactg caggccatgc
      481 gcagcgatgc gtcggcactg ctgcacgtct tcttcggcaa gggtagcggc ttctcgccac
      541 gccgcatcgg cgcgtgccga cggtggcagc gtctgcagca cggcctgcag gcgcgccagc
      601 gcccagcgca ggttgaccgc cgtcggccgt gtcgcggcca gcgtttcgat ggcgacggcg
      661 agtgtggcat cgtccgcccg ctgcgtcagt gccagggcga cgccataagc ggccgccacc
      721 ccgatcaacg gcgcgccgcg cacctgcatg ctgcggatcg cgtgcgccac ctcggcaagc
      781 gtacgcagcc gcaggatgcg gtacacgtgc ggcagcgccg tctggtcgat gatgtcgacg
      841 gcacgggcgt cggccactgg ctggatcgtt cgcgtcggcg tgccattgat taacatgccc
      901 gccattctag cagccggctg cccggcaccg aacttccatc acactcccgc gggtgcagat
      961 ccgaagccct gcagggctcc ctccgcacgt caacgccgtt cgggtttaat atgccatggt
     1021 cgcttgtccg cgtattttcc ccactgagga acgcccatga aactgccatc ccgggtcaag
     1081 gcccttcgca cccctaccct gctgttctgc gctctcgccg ccggtcacct ggcaggaagc
     1141 ctgcaggccg ccgagatgcc caaacgcaag gccggcctgt gggagatcaa cacgcgcatg
     1201 gacggcatgc cggcaatggg cgcgatccag caatgcgtcg accagaagac cgacgacctg
     1261 atgcaacagc aggcccggaa ggagcaaccc aactgcagcg tcatcgacgt caataaccag
     1321 ggcaacaagg tgagcgtgca ctcggtctgc aaggtgcagg gcagtaccgc caccaccgac
     1381 ggcgtattcg tgggctcgtt cgacgccgcc tacaagggag acatgttgac ccgcttcaat
     1441 ccaccgctgc atggcaccaa ggagtcgaag gtctcgctgg aagcgcgctg ggtcggaccg
     1501 tgcaaggccg gtcagaaacc cggcgatgtg atcatgccga acatggcagg gagcaacctc
     1561 aaggaaatga tgaaggatcc gacggtccag gagatgatga agcggcagaa atagagctgc
     1621 tgcatggggc tccggcaggc atacgcggtc gccccgcctc gccgcaagtc ccttacaata
     1681 atggcctgca tgctgggcgg gggttggtcg gcgacgcggc agaatgggca cggcaaggta
     1741 ctcctgcggt gacatgagcg tcgacgtgca tggacagaac ctgcgctcaa atggggagag
     1801 aggacgccga ttgccgtgca agtcgccttg tcgcgccaca ttcgggttga tcggcggtcc
     1861 agcggtttca tcaacaagaa aggagcaaac atgtcttatt cggccttcgt acaggcacgc
     1921 gatttccttc tggctcaccg caccgactac gaaaccgcct atcgcgattt ccagtggccg
     1981 caactgggcg aattcaactg ggccctcgat tatttcgatg tgatggcagc gggcaacgat
     2041 cgcccggcgc tgtgggtggt caatgaagac ggctcggaac agaagatgtc gttttccgag
     2101 atgtcccggc gttcgaacca ggtcgcgaac tggctgcgcg cgcaaggcgt caagcgcggc
     2161 gatcgcatcc tgatgatgct cggcaacgag gtgccgctgt gggatacgat gctggcatcg
     2221 atcaaactcg gcgccgtgtt gtcgccggcc accacgctgc tggctcccga cgacttgcgt
     2281 gaccgcctcg accgcggcca ggtacgacac gtgatcatcg gccacacgca cgccgaaaag
     2341 ttcagcgacc tgcctggcga ctacacacgc gtcaccgtcg gcggcgcggt ggccggctgg
     2401 cagcagcttg aacaggccta cgagggatcc agcgacttcc agcccgacgg gccgacacag
     2461 gcgaccgacc cgcagcttct gtactttacg tccggcacca cctcgaaacc caagctggta
     2521 ctgcacagcc agcaaagcta tccggtcggg cacctgtcga cgatgtactg gctcgggctg
     2581 caacccggcg acgtgcactg gaacatcagt tccgccggct gggccaagca tgcctggagc
     2641 tgcttctttg cgccctggaa tgccggcgcc acggtgttca tctacaacta cgcccgcttc
     2701 gcggcagcat cgatcctgga aaccctggtc agatgttcgg tgaccaccct gtgtgcgcca
     2761 ccgacggtct ggcggatgct gatccaggag gacctgagca agtatccggt caaggttcgc
     2821 gagctgatcg gcgccggcga gccgctgaac cccgaagtca tcgatcaggt caaggaggcg
     2881 tggggcatta ccctgcgcga cggcttcggc cagaccgaaa cgaccgcaca ggtcggcaac
     2941 tcgcccggac aggcggtcaa gctcggctcg atgggacggc cgatgcccgg ctatcaggtg
     3001 cgcctggtcg atatggacgg ccatgtgagt gacgaaggtg aaatctgtct gaccttgtcg
     3061 ccgcgtccgc tcggactgat gatctcctac gacgacgacc ccgagaaaac ggccgaggtg
     3121 atgcgcgacg gtttctatca caccggcgac acggccgcga tcgatgccga cggttacatc
     3181 acctacgtcg gccgtgccga cgacgtcttc aaggcgtctg actaccgcat cagcccgttc
     3241 gaactggaaa gcgtcctgat cgaacacgag gcggtcgccg aagcggccgt ggtgccgagt
     3301 cccgatgcct tgcgtctggc ggtaccgaag gccttcctga ccttgcgcgc aggctatgaa
     3361 ccgagtcggg aactggcgcg cgagatcttc gtctttctgc gcagccgtct gtcaccttac
     3421 aagcgcgtgc gtcggctcga attcggcgaa ctgccgaaga ccatttccgg caagatccgc
     3481 cgcgtcgagt tgcgcaaggg cgagcaggac aaggccgccg gaccacgtgg tgccatggag
     3541 ttctacgagg aggatttcca gttctgagaa tcgccgtgta atctgctcgc catgcgcaca
     3601 gaccccatgc cgacgccctt ggcatggggt ttttcatttc cgggacggca tcgcccgccg
     3661 tggtacgcct gcctcaccgt tgcctacggc gcgacctgca ggaccgtcgg ggcgaagttc
     3721 acccgtcgcg tctcgacgat ccgcgtctcg tcgaccttcc cctcgacgat ctcgtaaccc
     3781 atcagcgcga agaccctgcc gcggacaaag agcggccgcg agttgccgta ccaatcgacg
     3841 catgaagcgc gacacgcatc gttgcggtcg ctggcggacg ggcgagcctt gagcgttccc
     3901 agttcactca gcaacagacc gcgattgcgc agaaaaagca gggccgccga ctcggtgccg
     3961 agttggcgga taccggactc gctacctccg acgatcggca ggccgagcag accgtccagt
     4021 tcgctttccg ccttgtagaa aaagccgtgg ctgcgcgttt caccctgcgc ggcgtcccgg
     4081 cgcgtgtact ggccgacggt catcggtagc gacccgaggc gcacgctggt gagatgcaga
     4141 tccttgccgt cactgccgac aaccagcgca ttgccgccga gcgcctccag acggtcaacg
     4201 gcgtgcggta aaggcaactc gtaaacctca ccctgcgcat agcgtaccgc gtagacctgc
     4261 gaacggttcg tcgcctgcgg cgcgtgccag ccggtcccgc caccgtagag cagaaacggg
     4321 cccacgtagc gactctggat ggcgtaaccc gctgctttcg gcaagcggcg atacgccgcc
     4381 atcggagcgc tctcacggcc atcggagaag ctcgccagcg gtacccgcag cagcgccagg
     4441 tcgccggcgt tgacctcggc ggcccacatg ccgtcgccgc ggccattgga acgcagcagc
     4501 acgttcagca tgccgtcgcc ttccaggaac gagaactggt cgatcggact gcccatggtc
     4561 ttcagcgcac tcggtgcgga accgtcgagc gggatacgga acaccgccga tgtcgcgacg
     4621 gcggcctgcg gcgcgcctcg ccacgccgtt gtccagacga acaccgcggt cggcgagacg
     4681 tagaagacgc gtcccggcgg ccccagcacg gccgtgctct ggcagtccat ttcgggcttg
     4741 gcgagatcgc agaccgtgac gctgtgcagc gcggcaccgg caaaggggtc gagcgcctcg
     4801 tcgctgcggt aaatccgggt cgccggggca attcgcctga agtcgctcgc ctgcgcgccc
     4861 tcccgccagc ggcgcatggc cgggaatgcc gcgtacggat ccccccatgg actgaggata
     4921 tgtggcgaat aaacgatcag cttgctgccg atcagtcggc tcgcgtagtt gcgcgacgaa
     4981 tagtagtcgt tggaacgtag gtgataggtc gcccgatagg ccagttgccc tgcacgcgaa
     5041 atgtcgaaca cgccgacttc ggtgccgcca cgcgcgtagc tgtaaccgat gacgacgatg
     5101 gtgttgtcgg aaatcaacat ctcgtcgtac cacgcaccgc cgggatccac atcgcgcccg
     5161 taggcgtcaa tcgccgaaac cggtcgcagt tggttgtcac cgatgcggat ggtgaacaaa
     5221 cgtccgcgac gcaggatcac cagatgctcc ccgtgcacct tgacgatgcc gccttcgtcg
     5281 acgccggcgt gttgcacgtt ggtcaccgac tcgttcttgg cacccgccgc ttccttggcc
     5341 accgccgcgg gcgcggcggc tgccggagcc ggcgcgggaa gcacctggcc aaccacgaat
     5401 tccgaagatt tgtcacggcg agcgcgttgc tcgtcgcggc gacgctgtgc ctcgtcagtc
     5461 cagcgcttga acaggcccgc gagttcctgt tcgctggcga aagcgctgag cgtcttgcga
     5521 ggcgaggcag ggtcttcggg cattgcggcc gctgccggtg aggaaacgaa cgcgctcagc
     5581 gcggcgacaa cgaggcaatt gacgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnctgct gcaggtggtc actgtactcc cagaagcgtg ggttgctgcg gcgaacgaca
     5761 tagcgatccg ccagggcatg gtagtcgctc tccgacgaca gttccctgat cgcctgggcc
     5821 agcacccgaa tttccgatcg ctgtacgcga tagatggcat tcggataggc accgatgaaa
     5881 cctgccgcga cggtcagggt attctcctcg ggtcgcaact cctgcccttc gcggagcagg
     5941 cttgatacat tgctgtgacc ggtgttgcgc aacaaggtga catagcgcgg cgcgcgctcg
     6001 ccctcatcga tgcgcaggta gaccagctcc ggcaaccagg acagcgccgg gccgcgcagt
     6061 tccgccagcg ccgcaagatc gttctgcagg ccgacatcgc ccagagccgg gagatcgtat
     6121 cggtgatcca gcacgggccc caggcgtttt ctgagcagcg tgtagaactc ctgctgagga
     6181 tcgtcgccct ggaagcggat gccggtctcg ctgtcgaggc tcgccagacg cccatagacg
     6241 tgctccttga cctcctcgct ggcaccttga taccagtaat cacgcacagc catgcgccgg
     6301 tcgcgaggca gaaaggcaat gaagttgaac tcgccctcca tgcgcatgaa gtccatatac
     6361 agccgggtca gcaactgatg acccacgttt ccgtaaacat cgaagccggc caccaggaga
     6421 tagtggatgc gctccagcaa gccgtagccg atcacccagg cggttttcgg cggctgaccc
     6481 accagtccct tgaccaccga cgcactgtcg aagtgacgga acaccgtcaa cgccgcgttc
     6541 gggttgcgac cattgccatc ccaaatccag ttcaggtccg gccggcgatt ggtcgcggta
     6601 aaacgcgtca gaaaatcaga cttcttctgc aggtagctgg cctcccgtcg cgcatattcg
     6661 cgccatggcc tgatgatccc cgcgtcactt cccagttccg ccgggagttt cagcaggctg
     6721 gcctcgcgcg ccaggaaatc gcccaactgc tgctgcgcat cgtcggcatt ggcttcgaaa
     6781 aagacccaga actggtcctc gatcacgttc agcgcgacct gaccacgaca gaccgggccc
     6841 ttgatgaaac ccatgatcgt gaattgtgct tcatcaagca tgaactgata tcgcgccgtg
     6901 gtcggcaacg cccggaacgc aatgaaggga ttggcggcca cgtcgagagc gtaggacggc
     6961 agttcgtcca cccggtaatc cggcgccagg aagagttcgc gccaacgcgc catgcgtgcc
     7021 tgcccaagcg catagggcat gtgtgtcttg tcgacgatgg tttccttttc gcgttccagc
     7081 cgatagtaga cgcgctctac gccggggtcg tcaaacggcc gtcggctggc aaccagatcg
     7141 atcggctg
//
LOCUS       DAOSIT010000035         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9672_length_7066_cov_9.006846, whole genome shotgun sequence.
ACCESSION   DAOSIT010000035 DAOSIT010000000
VERSION     DAOSIT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9672_length_7066_cov_9.006846"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLL14_01705"
     CDS             <1..402
                     /locus_tag="PLL14_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG02662.1"
                     /translation="LGAAEFCWHTARQYTLDRQQFGRPLGANQLIQKKLADMQTEITL
                     GLQGCLRLGRMKDEGSATPEITSMMKRNSCGKALDIARTARDMLGGNGISDEYGVIRH
                     LVNLEVVNTYEGTHDVHALILGRAQTGIAAF"
     gene            complement(421..558)
                     /locus_tag="PLL14_01710"
     CDS             complement(421..558)
                     /locus_tag="PLL14_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02663.1"
                     /translation="MTLCPIAIVAGCRKCPAFSLCPLKTVLGDAPKQEEAPTSPPEDK
                     K"
     gene            complement(621..1373)
                     /locus_tag="PLL14_01715"
     CDS             complement(621..1373)
                     /locus_tag="PLL14_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOG02664.1"
                     /translation="MIVARDIRKRFGVVEVLKGVSLEIAKGDVVAVIGPSGSGKSTFL
                     RCLNHLETVDAGEVLIAGETLVSSDAAGVCRYAPDAELRRICRRMGMVFQHFNLFPHL
                     TVLQNIIEAPLTVKGLARDAVVPKAEELLRKVGLFDKCDSYPARLSGGQKQRVAIARA
                     LAMEPDIMLFDEPTSALDPELTGEVLRSMRQLAEEHMTMLVVTHEMAFAREVSSSVVF
                     MDAGEIVEARAPAEMFSSPAHPRTQAFLEHML"
     gene            complement(1393..2046)
                     /locus_tag="PLL14_01720"
     CDS             complement(1393..2046)
                     /locus_tag="PLL14_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG02665.1"
                     /translation="MDYIIEILGPLLQGTAVTLQVFLLTLLLSVPLGLGLALVRVSRF
                     HLASAMVNGYIWLMRGTPLMLQMLFIYFALPFVPVIGVRLPDFPAAIVAFVLNYAAYY
                     AEIFRAGIQSIERGQYEAARVLGLSYGQTMRRIVLPQVVKRILPPMSNETITLVKDTS
                     LIYVLAMNDLLRAARGIVQRDFTTMPFVVAAAFYLLMTLVLTWGFHYLEKRHAVYDE"
     gene            complement(2194..2988)
                     /locus_tag="PLL14_01725"
     CDS             complement(2194..2988)
                     /locus_tag="PLL14_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG02666.1"
                     /translation="MKKSISLLLTTLATGVLLLGCGKQEAPQAAAAKPAAVTRIVVGL
                     DDNFPPMGFRDKENKLIGFDIDLAREAAKRLGAEVEFKPIDWNAKEAELNGKRVDALW
                     NGLTITEKRKENIAFTTPYLENRQIVVVVAKSPLKSKADLAGKVVGLQEGSSAVEAVE
                     KDAVAKSIKELKKYGDNVTALMDLSTGRLDALVVDEVVGRYYVAKKPGEYVVLEENFG
                     TEEYGVGVRKSDTELLAKLQKTMDEMKKDGSAARISTTWFGKDIIK"
     gene            complement(3161..>3678)
                     /locus_tag="PLL14_01730"
     CDS             complement(3161..>3678)
                     /locus_tag="PLL14_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG02667.1"
                     /translation="AVNVSMPQFRKADFVEQVADTLARFQMPASLLELEITESIAMDE
                     PKVVLQGLQALKRLGVQIAIDDFGTGYSSLGHLQSLPIDCLKVDRSFVKEIRDGQGGV
                     FAETIVGLSDKLGVKSVAEGVETLEQAGFLRRLGCAVAQGYLYAPPMPAEPLHAWLHQ
                     QARSRRDPRPV"
     assembly_gap    3679..3778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3779..4900)
                     /locus_tag="PLL14_01735"
     CDS             complement(<3779..4900)
                     /locus_tag="PLL14_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOG02668.1"
                     /translation="MLDELVPFGDDIQEHESPPSGGTPWKVLVVDDDDEVHDVTRFVL
                     HDLRIFGRPLNLLFAHSARQARSVLRQHPDTAVALLDVVMETERAGLDLVGYLRDELG
                     LAECRIILRTGEPGYAPELSVMNEYDINDYRTKSELTHTRLITTVSAALRAYEQLRAI
                     AEHRRGLELIVHAAADLLEQHAVADLAEGVLTQLAALLKLPAEGIVCTQKGSPFGDDG
                     EHCYVVGAAGRHAPYIAQPLEALPEAAIVAAIQNSVARRQHVFAADHTVLYLKAAGNQ
                     EAAVYLESGHVAAALDRPLLDVFVANIAACFGNVKLVERLNHIAFHDPLTRLPNRVQF
                     IADLDGVAGRDADTVVALLDIDHFADLNDGLGQDIGNALL"
     gene            5129..6292
                     /locus_tag="PLL14_01740"
     CDS             5129..6292
                     /locus_tag="PLL14_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG02669.1"
                     /translation="MRSPPLAGLRVLDLTRLLPGPLASMHLADLGADVIKIEDTGAGD
                     YARSAGTVHGDVSYFQLVNRNKRSLSLDLKQAAGVEAFMRLAATADVILEGFRPGVVD
                     KLGIGYASVAAINERIVYCAITGYGQDGPYRDRAGHDLNYLGYCGLLDQIGVAGSAPA
                     IPNFQIGDLLGGSLSALVGLLAAVVDARATGRGRYVDVAMTDAVFAHTIFPLLAVLGL
                     GQALPRGEDVLSGGMPSYGVYATSDGRHLAVSAMEPKFWQMLCSAIDRPDLKPFAFAT
                     GAEGLRIRGELAAAFAERSFADWTAIFERVDCCVSPVLRFEESLENEQLRARAMIVEV
                     AGVKQFAPPFKISELPFETRLPPPAAGEHSEEILLEAGFSTGEIARLRTQKVI"
     gene            complement(6344..6943)
                     /gene="mobA"
                     /locus_tag="PLL14_01745"
     CDS             complement(6344..6943)
                     /gene="mobA"
                     /locus_tag="PLL14_01745"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HOG02670.1"
                     /translation="MRVDGITGVVLAGGLGRRMGGLDKGLQELAGRPMIAWVIERLGP
                     QVDELLINANQNGERYSAFGHRVVPDQVADFAGPLAGLHAAMSVARHPLVATAPCDSP
                     FLPADLVARLQQALTASGASLAVVRTGAQPQPVFSLCRRELLPSLGDYLGNGGRKVAR
                     WHDTLTVVEVAFDDQVAAFENINTCADLQRLATNPARTP"
BASE COUNT         1257 a         2258 c         2219 g         1232 t
ORIGIN      
        1 ctcggcgccg ccgaattctg ctggcacacg gcgcggcagt acacgctcga tcgccaacag
       61 ttcggtcgtc cgctgggggc caaccaattg atccagaaaa agctcgccga catgcagacc
      121 gaaatcaccc tcggcctgca aggctgcctg cgcctcggac ggatgaagga cgagggtagc
      181 gccacgccgg agatcacctc gatgatgaaa cgcaattcgt gcggcaaggc cctcgacatc
      241 gcccgcaccg cccgcgacat gctcggcggc aacggcatct ccgatgaata tggtgtcatc
      301 cgccatctgg tcaacctcga ggtggtcaac acctacgaag gaacacacga tgtgcatgcg
      361 ctgatcctcg gccgggcgca gaccggcatc gcggcgttct gagaccggcg cgcggcggcc
      421 ctacttcttg tcctctggcg gggaagtcgg cgcctcctcc tgcttcggcg catcgccgag
      481 cacggttttc aggggacaga gggaaaacgc cgggcatttc ctgcagccgg cgacaatggc
      541 gatcgggcac aaggtcatgg catgtctcct tcggttgaga gtgggcagct agcatacacc
      601 atcgactgcg ccgccgggcg ctacagcatg tgctcaagaa aggcctgtgt acgcggatgc
      661 gcggggctgg aaaacatttc ggcgggcgcg cgcgcttcga caatttcgcc cgcgtccatg
      721 aacaccacgc tgctggagac ctcgcgggca aaagccattt cgtgagtgac cacgagcatg
      781 gtcatgtgct cttcggccag ctggcgcatg ctgcgcaaca cttcgccggt caattcgggg
      841 tcgagcgccg aggtcggctc gtcgaagagc atgatgtccg gttccatggc cagcgcgcgg
      901 gcaatcgcga cgcgctgctt ctgcccgccc gaaagacgcg ccggataact gtcgcacttg
      961 tcgaacagac ccaccttgcg cagcaattct tcggccttgg ggacgaccgc gtcgcgcgcc
     1021 aagcccttga ccgtcagcgg cgcctcgatg atgttctgta acaccgtgag atgcggaaac
     1081 aggttgaaat gctggaacac catgcccatc cggcggcaaa tgcgacgcag ttcggcatcc
     1141 ggcgcgtagc ggcagacgcc ggcggcgtcg ctgctcacca gggtttcgcc ggcgatcagc
     1201 acttcgccgg cgtcgacggt ttcgaggtgg ttgaggcagc gcaggaaggt gctcttcccg
     1261 gaacctgacg gaccgatcac ggcgaccacg tcgcccttgg cgatctccag agaaacgccc
     1321 ttcaacacct cgacaacgcc aaaacgcttg cgaatgtcgc gggccacgat catcggagcg
     1381 acgttcgcca gctcattcgt cgtagacggc atgtcgcttc tccaggtaat ggaagcccca
     1441 ggtgagcacc agtgtcatca gcagataaaa ggccgcggcg acgacgaatg gcatggtcgt
     1501 gaaatcgcgc tgcacgattc cccgcgcggc gcgcagcagg tcgttcatcg ccaggacgta
     1561 gatcagcgag gtgtccttga ccagggtgat ggtctcgttg ctcatcggcg gcagaatgcg
     1621 cttgaccacc tgtggcagga caatgcggcg catggtctga ccatagctca ggccgaggac
     1681 gcgggcggcc tcgtactgcc cgcgttctat cgactggata ccggcgcgga agatctcggc
     1741 ataataggcg gcataattga gcacgaaggc gacgatggcg gccgggaaat cgggcagccg
     1801 cacgccgatc accggtacga acggcaaggc gaagtagatg aacagcattt gcagcatcag
     1861 cggcgtgccg cgcatcaacc agatgtaacc attgaccatc gcgctggcca ggtgaaagcg
     1921 ggaaacccgc accagcgcca ggcccagccc caacgggacg gacagcagca aggtcagcag
     1981 gaagacctgc agcgtcaccg ccgtgccctg cagcagcggg ccgaggattt cgatgatgta
     2041 gtccatgcca tccgccttgt cgccgggatg cacaaccggc gagcgcccct ggcgctgagc
     2101 cgcgcaccag gacggtggtg aagtggaatc gacagggcgg tgcacgccct gtcgcctgca
     2161 gaaagcgact gccgttcggt gggcgggcca ggcttacttg atgatgtcct tgccgaacca
     2221 ggtcgtcgag atacgggcgg cggaaccgtc cttcttcatc tcgtccatgg tcttctgcag
     2281 cttggcgagc aactcggtat cgctcttgcg gactccgacg ccgtactcct cggtgccgaa
     2341 attttcttcg agcacgacgt attcgccggg cttcttggcg acgtagtaac gaccgaccac
     2401 ttcgtccacc accagcgcgt cgaggcggcc agtggagaga tccatcagcg ccgtgacgtt
     2461 gtcgccgtac ttcttcagtt ccttgatcga ttttgcgaca gcgtccttct cgaccgcctc
     2521 gaccgcgctg ctgccttcct gcagaccgac caccttgccg gccaggtcgg ccttgctctt
     2581 cagcggcgac ttggcgacga ccacgacaat ctggcggttt tccaggtacg gcgtggtgaa
     2641 ggcgatgttc tctttgcgct tctcggtgat cgtcagaccg ttccacaagg catctacccg
     2701 tttgccattg agttcggctt ccttggcgtt ccagtcgatg ggcttgaact cgacctcggc
     2761 gcccagccgc ttggccgctt cacgcgccag gtcgatgtcg aaaccgatca acttgttctc
     2821 cttgtcgcgg aaacccatcg gcggaaaatt gtcgtcgagg ccgacgacga tccgggtgac
     2881 cgcagccggc ttggccgccg cggcttgcgg cgcttcctgc ttgccacagc caagaagcag
     2941 gacgccggtc gccagcgtgg tgagcaacaa tgaaatgctt ttcttcatgc gtgtttacct
     3001 tgattgatgg gaggtgaaaa aaggcctcgg gtcgcctgct gtagtggcag ttaccccggt
     3061 tctggggtgc gactttcgag caacctgcaa ttatcccgga aaaaattgcc cttcgccggc
     3121 gttggcgacg attttcccgg tagcggccgg ggcgcggcct tcaaaccggg cgaggatcgc
     3181 gacgtgatct ggcctgctgg tgcagccagg cgtgcaaggg ttcggcgggc atcggtggag
     3241 cgtacaggta accctgcgcc actgcgcacc ccaggcgacg cagaaagccc gcctgctcca
     3301 gagtctcgac gccctcggcg accgacttca cccccagttt gtcgctcaag ccgacgatgg
     3361 tttcggcaaa gacgccgccc tggccatcgc ggatttcctt gacgaaggaa cggtcgacct
     3421 tgaggcaatc gatcggcagt gactgcaggt gccccaacga ggaatagccg gtgccgaagt
     3481 cgtcgatggc aatctggacg cccagtcgtt tgagcgcttg cagtccctgc aacaccacct
     3541 tcggctcatc catggcaatg ctttcggtga tttccagttc gagcagtgag gccggcatct
     3601 ggaagcgcgc aagcgtatcg gcgacctgct cgacgaaatc ggccttgcgg aattgcggca
     3661 tgctgacgtt gaccgccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     3781 gcagggcgtt gccgatgtcc tggccgaggc cgtcgttgag atcggcgaaa tggtcgatat
     3841 cgagcagggc cacgacggtg tcggcatccc ggccggccac gccatcgagg tcggcaatga
     3901 actgcacgcg gttcggcagc ctcgtcagcg gatcgtgaaa agcgatgtgg ttgaggcgct
     3961 cgaccagttt gacattgccg aaacaggcgg cgatgttggc cacgaagacg tcgagcagag
     4021 gccggtcgag agccgccgcg acgtgcccgg actcgagata cacggcggct tcctggttcc
     4081 cggcggcctt caggtagagc acggtgtgat ccgcggcgaa gacgtgctgc cgacgcgcca
     4141 cgctgttctg aatcgccgcg acaatggccg cttccggcaa cgcctccagt ggctgcgcga
     4201 tgtaaggcgc gtgccgacca gcggcaccga ccacgtagca gtgctcgccg tcgtcgccga
     4261 acggcgatcc cttctgcgta cagacgatgc cttccgcggg cagtttgagc agcgcggcga
     4321 gctgggtgag cacgccttcg gccaggtcgg cgacggcatg ctgctcaagc aggtcggctg
     4381 ccgcatgcac aatcagttcc agcccacgcc ggtgctcggc gatggcgcgc agttgctcgt
     4441 aggcacgcag cgcggcgctg accgtggtga tcagacgggt atgggtgagt tcgctcttgg
     4501 tccggtagtc gttgatgtcg tattcgttca tcaccgacag ttccggcgca tagcccggct
     4561 cgccggtgcg caggatgatg cgacattcgg ccagacccag ttcgtcgcgc aggtatccca
     4621 ccaggtcgag cccggcgcgc tcggtttcca tcaccacatc cagcagggct accgcggtat
     4681 cgggatgctg gcgcagaacc gagcgagcct ggcgggcact gtgcgcgaac aacaggttca
     4741 ggggtcgccc gaaaatccgc agatcgtgga gcacgaagcg ggtgacgtca tgcacctcgt
     4801 catcgtcatc gaccaccagc accttccatg gcgtaccacc tgacgggggt gactcgtgtt
     4861 cctggatgtc atccccaaac gggaccaatt cgtcaagcat gcaggcactc cactgtaggg
     4921 ccctcggagg cggtccgggg gatcgctgag gtcaaggtgt aggcttggcc cgcgttgctg
     4981 tatcccggcc tcgtctcgcc aagtagacca gtggtcggat tatagacaat atagcggccg
     5041 cgcccacagc gacaatcgcc atgccggctg ggccggcgtt ctgcgccggc cgcccggccc
     5101 gccatccacc atcccgaaag caatcaccat gcgttctccc cctctcgccg gcctccgcgt
     5161 cctcgatctg acgcggctgt tgccggggcc cctggccagc atgcacctcg ccgaccttgg
     5221 tgccgacgtg atcaagatcg aagataccgg cgccggtgac tacgcgcgca gcgccggcac
     5281 cgtacacggc gacgtcagtt acttccagct cgtcaaccgc aacaagcgca gcctcagtct
     5341 cgacctcaag caggcggccg gcgtcgaggc cttcatgcgt cttgccgcca ccgccgacgt
     5401 gattcttgag gggtttcgac ccggcgtggt ggacaagctc ggcatcggct acgcgagcgt
     5461 ggccgcgatc aatgaacgca tcgtgtactg cgcgattacc ggctacggtc aggatggacc
     5521 ctatcgggac cgcgccgggc acgacctgaa ctatctcggc tactgtggcc tgctcgacca
     5581 gattggcgtc gccggcagcg cgccggcgat cccgaacttt cagatcggcg acctgctcgg
     5641 cggctcactg agtgcgctgg tcggcctgct cgccgcggtt gtcgatgcgc gcgccacagg
     5701 tcgtgggcgc tatgtcgacg tggcgatgac cgatgccgtt ttcgcgcata cgatctttcc
     5761 gttgctcgcc gtactgggcc tggggcaggc actgccgcgc ggcgaagatg tcctctccgg
     5821 cggcatgccc agctacggcg tctatgccac cagcgacggg cgccatctgg ccgtcagcgc
     5881 gatggagccg aagttctggc agatgctgtg ttcggccatc gatcgtcccg atctcaaacc
     5941 ctttgccttc gccaccggcg cggagggtct gcgtatccgt ggcgagctcg cagcggcttt
     6001 cgccgaacgc agtttcgccg actggacggc gatcttcgag cgcgtcgact gctgcgtctc
     6061 accggtgctg cgtttcgagg aaagcctgga gaacgagcaa ctgcgcgcgc gcgcgatgat
     6121 cgtcgaggtg gccggagtca aacaattcgc gccaccgttc aagatcagcg aactgccttt
     6181 cgaaacccgc ctgccaccac cggccgccgg cgagcacagc gaggaaatcc ttcttgaggc
     6241 tgggttcagc acgggagaaa ttgcccggtt gcgcacgcag aaagtgatct gacccgctga
     6301 ccaggatgcc ggatgggcag ccttgccggg cgcgcgcccg cctctacggc gtgcgcgccg
     6361 ggttcgtcgc cagacgctgc agatccgcac aggtattgat gttttcgaaa gctgccacct
     6421 ggtcatcaaa tgcgacctcg accaccgtca gcgtatcgtg ccagcgcgcg accttgcgcc
     6481 cgccattgcc gagataatct cccagcgacg gcaggagttc gcgtcggcac agcgaaaaca
     6541 ccggttgcgg ttgcgcaccg gtgcgtacca ccgcgaggga agcaccactc gcggtcaagg
     6601 cctgttgcag gcgcgccacc aggtcggcgg gaagaaaggg ggaatcgcag ggagccgtgg
     6661 cgaccaaagg gtgccgggcc accgacatcg cggcgtgcag accggccagc ggcccggcga
     6721 aatcagcgac ctgatcgggc acgacacgat ggccaaaagc gctgtagcgc tcgccgttct
     6781 ggttggcgtt gatcagcagt tcgtcgacct gcggaccgag ccgctcgatc acccaggcaa
     6841 tcatcggtcg cccagcgagc tcctgcagac ctttgtcgag cccacccatg cgccgaccga
     6901 gcccgcccgc gaggacgacc cccgttatcc catcgacgcg caaggcgcat gcctcctgcc
     6961 gcccgcgccg ccgaccctcg gacgctgagc gagggtcatt cgccgtgctc gccggcgcgg
     7021 cgtggcaccg cggcctgtgg cgtgggctcc gctggccgca gggcaa
//
LOCUS       DAOSIT010000036         7064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9674_length_7064_cov_10.264660, whole genome shotgun sequence.
ACCESSION   DAOSIT010000036 DAOSIT010000000
VERSION     DAOSIT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9674_length_7064_cov_10.264660"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLL14_01750"
     CDS             complement(<1..881)
                     /locus_tag="PLL14_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HOG02671.1"
                     /translation="MADYSLPLRRYYAYYTVGFFLFILMLGVLERHGMPPRWIGYSFL
                     FLTIFLYATIGVLARTASVSEYYVAGRRVPAVFNGMATGADWMSSASFMGLAGTLYLT
                     GYQGLAYVMGWTGGYVLVALLLAPYLRRFGQYTIPDFLAARYGGNLARLVGVFATVVA
                     SFVYVVAQIYGVGLITSRFVGLQFEVGVFVGLAGILVCSFLGGMRAVTWTQVAQYAIL
                     IVAYLVPVTILSYEVTGIPVPQLTYGQVLQKVQVLEERIFDDPAEVDARRLFRERADG
                     YYTRILTLPDSLEEERR"
     gene            complement(897..1610)
                     /locus_tag="PLL14_01755"
     CDS             complement(897..1610)
                     /locus_tag="PLL14_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG02672.1"
                     /translation="MTWFSRLFAGPGAPGVALTARQQQLIAAWQQLPAPDLRRSHYRC
                     RYVVVDVETSGINVRTDRLYAIGAVALVDGQIDFRDTLQLILAAAASTSEVAAATSDA
                     ASAPVVGPQAVEALIAFLQFVGKAPLVAYNVPFVAAMVERVLAECLGIELALPWIDLE
                     WVMPDLFRDIDSTQGRLDAWLTHFEIDSIHRHNAVSDAYATAQLLQVTIARGARKGFE
                     NPASLIELEKARRHLHQSS"
     gene            complement(1618..3528)
                     /locus_tag="PLL14_01760"
     CDS             complement(1618..3528)
                     /locus_tag="PLL14_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HOG02673.1"
                     /translation="MNNSATEMLVAACIEFLQAYPPFDRMEAEALRFLAEHVRLAHYP
                     KGALILSSEMGVAPVLYILQRGKVRVRASGGAGNVEFSAITLGPGQAFAIGALMAQRP
                     TSSAYVAREDVFCYELPADDFFTLTQQSTVFSLFCSQYIAGLLKQSQQQLQLQFAQRA
                     AEQKTMNSPLAVIVKREPVSVPADASIRQVVELMADRHLGSMVIVDAGEQPVGIFTLS
                     DVLKRIVLPGRSLDQPISSVMSTSPQTLPLAANAHDAALAMAMHGIRHVLAVDEGGRL
                     KGVVSERDLFKLQGAGLRQIRHAIDAATSLDVLQHAREDVRQLSLTMLTEGGGAQEIT
                     QFISTLNDTVTRRVIELNLDRHDLYGIDWAWLSFGSEGRDEQTFTTDQDNGIVFICTD
                     IMDRELTQLRFLEFARDVNADLDTCGFPLCKGNIMASNPALCLTLEEWEEKFAHWVRT
                     PEPLALLNATIFFDFRPLYGRFNLAHRMRLSLLRQTRGNPLFLRMLAQNALSVSPPLG
                     RIRDFVTGADPEHPGTIDLKKYGVRLFTDAARVFALAHQIEATNTVSRLKRASTLVNF
                     SSDDLAASLEGFNFIQLLRLRHQHFEQEHGRPGDNLIRPDELNEIERRILKEAFRQAR
                     KLQARLKLDYQL"
     assembly_gap    3548..3647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3648..5344)
                     /gene="acs"
                     /locus_tag="PLL14_01765"
     CDS             complement(<3648..5344)
                     /gene="acs"
                     /locus_tag="PLL14_01765"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOG02674.1"
                     /translation="MSLNEAVNFFEPSAEFVKQATISGMDAYRALCAEAEKDYTGYWA
                     RLAREHVSWKTPFTQVLDESNAPFFKWFADGTLNVSYNCLDRNVEAGLGDKVAIVFEA
                     DDGEVKKVTYKELLSRVSQFANGLRARGIKKGDRVVIYMSMGVEGIIAMQACARIGAI
                     HSVVFGGFSAQSVRDRVNDAGAVAVITADEQCRGGKKIPLKPAVDDGIALGGCESVKT
                     VIVFQRTGGTCHMVPGRDVWWHDVIAGQSDICEPEWVGAEHPLFLLYTSGSTGKPKGV
                     QHSTGGYLLHAIVTMKWTFDLKPNDLFWCTADIGWVTGHTYITYGPLACGGTEMVFEG
                     IPTYPDAGRFWKMIQDHKVTVFYTAPTAIRSLVKAADTNPAVAPTQYDLSSLRLLGTV
                     GEPINPAAWQWYYDSVGGSRCPIVDTFWQTETGGHMITPLPGVTPLVPGSCTLPFPGI
                     QAAVVDETGTELDWGKGGLLVVKRPWPAMIRNIWGDPERFKKSYYPDDFNGKLYLAGD
                     GSIRDVNTGYFTITGRIDDVLNVSGHRMGTMEIESALVSHPMVAEAAVVGRPDDLTG"
     assembly_gap    5731..5830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6013..>7064
                     /locus_tag="PLL14_01770"
     CDS             6013..>7064
                     /locus_tag="PLL14_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG02675.1"
                     /translation="MTTIKQEDFIQSVADAFQYISYYHPLDYILALGAAYEREQSPAA
                     RDAIAQILTNSRMCAEGHRPICQDTGIAVIFLKVGMDVHWEAGLSVQEMCDEGVRRAY
                     NNPDNKLRASVLLDPAGARRNSRDNTPAVVHYEIVPGHHLEVICAAKGGGSENKSKFY
                     ALNPSDSIVDWVLKTVPTMGAGWCPPGILGIGIGGTPEKAMLLAKESLMAPVDIHELI
                     ARGPKNRVEELRLELYEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAMIPNC
                     AATRHAHFHLDGSGPAVLTPPDLNDWPKVNWTPNTETSTRVNLDTLTAAEVAAWKPGQ
                     TLLLNGKMLTGRDAAH"
BASE COUNT         1382 a         2151 c         2118 g         1213 t
ORIGIN      
        1 ctgcgccgct cttcctcaag cgagtcggga agggtgagga tgcgcgtgta gtagccatcg
       61 gcgcgttcgc gaaacagacg gcgggcgtcg acttcagccg gatcgtcaaa gatcctctcc
      121 tcaagaacct gcactttctg cagcacctgg ccgtaggtca actgcggaac cgggatgccg
      181 gtaacctcgt aggagagaat cgtcaccggc acgagatagg cgacgatcag gatcgcgtat
      241 tgcgccacct gcgtccaggt cacggcccgc atgccgccca ggaacgagca gaccagaatt
      301 ccggcgaggc cgacaaagac gccgacttcg aactgcagac cgacaaagcg gctggtgatc
      361 aggccgacgc catatatctg cgccactaca tagacaaaag acgccacgac ggtggcgaag
      421 acgccaacca gccgtgccag gttgccgcca tagcgcgcag cgagaaaatc cggaatcgtg
      481 tattgcccga aacggcgcag gtaaggcgcc agcagcagcg cgaccagaac ataaccgccg
      541 gtccagccca tcacgtaggc caggccctga taacccgtca ggtagagcgt cccggccagt
      601 cccatgaagg aagccgaact catccagtcg gcgccggtgg ccattccgtt gaaaaccgcg
      661 ggcacgcgcc gaccagcgac gtagtactcc gagacactgg cggtgcgcgc cagcaccccg
      721 atcgtggcgt aaaggaaaat ggtcaggaag agaaacgaat aaccgatcca gcgcggcggc
      781 atgccgtggc gttcgagcac ccccagcatc aggatgaaca ggaaaaaacc gaccgtgtaa
      841 taggcgtaat agcgacgcag cgggagagag taatctgcca tcgcggagcc gccctcctag
      901 ctgctctggt gcagatgacg gcgcgccttc tcaagctcaa tcagactggc cgggttttcg
      961 aagcccttgc gtgcgccacg ggcaatcgtg acctgcaaca actgggccgt ggcgtaggcg
     1021 tcggagacgg cgttgtggcg gtggatactg tcgatttcga aatgcgtgag ccaggcgtcc
     1081 aggcgccctt gcgtactgtc gatgtcgcga aacaggtcgg gcatgaccca ttccagatcg
     1141 atccacggta acgcgagctc gattcccagg cactcggcga gcacgcgctc gaccatcgcc
     1201 gccacgaacg gcacattgta ggcgaccagt ggcgccttgc cgacaaactg cagaaaggcg
     1261 atcagcgcct cgaccgcctg cggcccgacc accggcgcag acgctgcgtc gctggtggcc
     1321 gctgccactt cgctggtcga tgcggcagcg gccaggatca gttgcagggt atccctgaaa
     1381 tcgatctgtc catcgaccag cgccaccgcg ccaatcgcgt acaagcggtc ggtcctgaca
     1441 ttgatgccgc tggtttcgac gtcgaccacc acgtagcggc agcgatagtg cgagcggcgc
     1501 aggtccggcg cgggcaattg ctgccaggcg gcaatcaact gctgctgcct ggcagtcagg
     1561 gccacgcctg gagcgcccgg gccggcgaag agtctggaga accacgtcat gagaggatca
     1621 gagctgataa tcgagtttca ggcgcgcctg caatttacgc gcctgtcgga atgcttcttt
     1681 gagaatgcgc cgttcaatct cgttcaattc gtccggacgg atcagattgt ctcccgggcg
     1741 cccgtgttcc tgttcgaaat gctggtgtcg caggcgcagg agctggatga aattgaagcc
     1801 ctccaggctg gccgccaggt catccgaaga aaaattcacc agggtgctgg cacgcttgag
     1861 acggctcacg gtgttggtcg cctcgatctg gtgcgccagc gcaaagacgc gcgctgcgtc
     1921 cgtgaacaga cgcaccccat atttcttgag atcaatcgta ccgggatgct cggggtcggc
     1981 gccagtcacg aaatcgcgta tccggcccag cggcggcgag acgctgaggg cgttttgcgc
     2041 cagcatgcgc aggaacagcg gattgcctcg cgtctgccgg agcagtgaga gacgcatgcg
     2101 gtgggccaga ttgaacctgc cgtaaagcgg ccgaaagtcg aagaagatcg tggcgttgag
     2161 cagggccagc ggttccggtg tgcgcaccca gtgggcaaac ttttcctccc actcctccag
     2221 cgtcaggcac agggccgggt tgctggccat gatgttgccc ttgcacagcg ggaaaccaca
     2281 ggtatcgagg tcggcgttga catcgcgggc aaattccaga aagcgcaact gcgtcagttc
     2341 gcgatccatg atgtcggtgc agatgaagac gatgccatta tcctggtcgg tggtaaaggt
     2401 ctgctcgtcg cgcccctcgg agccgaacga cagccatgcc cagtcgatgc cgtagaggtc
     2461 gtgccgatcg agattcagtt cgatgacccg acgggtcact gtgtcgttca gcgtcgagat
     2521 gaactgcgtg atctcctgcg caccccctcc ctcggtgagc atcgtcagcg acaattgccg
     2581 gacatcctcg cgggcgtgct gcagcacatc aaggctggtc gccgcatcga tcgcatgccg
     2641 gatctgccgc aggccagcac cttgcagctt gaacaggtcg cgctcggaaa ccacgccctt
     2701 gaggcgtccg ccctcatcca ccgccagcac gtggcggatg ccatgcatgg ccatcgccag
     2761 cgccgcatcg tgggcgttgg ccgccagtgg cagagtctgt ggcgacgtag acattaccga
     2821 ggagatgggc tgatcgagcg accggccggg gagcacgatg cgcttcagaa catccgagag
     2881 ggtaaagatc ccgaccggct gctcgccggc atcgacgatc accatcgacc ccaggtggcg
     2941 gtcggccatc agttcgacca cctggcgaat cgacgcgtcc gccgggaccg aaaccggttc
     3001 gcgcttgacg attaccgcca gcggcgaatt catcgttttc tgttcggcgg cgcgctgtgc
     3061 aaactggagt tgcagctgtt gttgcgactg cttgagcaag ccggcaatgt actgcgagca
     3121 gaacaggctg aagaccgtac tctgctgggt gagggtgaag aaatcgtcgg ccggcagctc
     3181 gtagcagaaa acatcctcgc gcgccacgta agcgctcgaa gtcgggcgct gcgccatcaa
     3241 cgccccaatg gcgaaggcct ggccgggacc gagcgtgatc gccgagaatt cgacgttgcc
     3301 ggcaccaccg ctggcccgca cccgcacctt gccgcgctgg agaatataaa ggaccggcgc
     3361 cacccccatc tcgctcgaca ggatcagcgc gcctttcgga tagtgtgcca gccggacatg
     3421 ctcggcaaga aagcgcagtg cctccgcctc catccgatca aaaggcgggt aagcctgcag
     3481 gaactcgatg catgccgcga ccagcatctc cgtcgcactg ttgttcatgc ctgctcctcg
     3541 atcggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg ccggtcagat
     3661 cgtcggggcg accgacgacg gccgcttccg ccaccatcgg atgggacacc agcgcggact
     3721 cgatttccat ggtccccatg cggtggcctg aaacgttcag cacatcgtcg atacgaccgg
     3781 tgatcgtgaa atatccggtg ttgacatcgc gtatcgagcc gtcgccggcg aggtagagct
     3841 tgccgttgaa atcgtccggg taatacgact tcttgaagcg ctccggatcg ccccagatgt
     3901 tgcggatcat cgccggccag ggtcgcttga cgaccagcag gccgcccttg ccccaatcga
     3961 gctcggtgcc ggtttcgtcg accaccgcgg cctggatgcc cgggaagggc agggtgcagg
     4021 agccgggcac cagcggggtt acgcccggca gcggcgtgat catgtgccca ccggtttcag
     4081 tctgccagaa cgtgtcgacg atcgggcagc ggctgccacc gacgctgtcg taataccact
     4141 gccaggccgc cgggttgatc ggctcgccga ccgtgccgag cagtcgcagc gacgacaggt
     4201 catactgggt cggcgctact gccgggttgg tatcggcggc cttgaccaac gaacggatgg
     4261 cggtcggtgc agtgtagaag acggtgacct tgtgatcctg gatcatcttc cagaagcggc
     4321 ccgcatccgg ataggtcggg atgccttcga agaccatctc ggtaccgccg caggcaagtg
     4381 gtccataggt gatgtaggta tggccggtaa cccagccgat gtcggcggtg caccagaaca
     4441 ggtcgttcgg cttgaggtcg aaggtccact tcatggtcac gatggcgtgc aacaggtacc
     4501 cgcccgtcga gtgttgcacg cccttcggct tgcccgtcga acccgaggtg tagagcagga
     4561 acaaggggtg ctcggcaccg acccactccg gctcgcagat gtcggactgc ccggcgatca
     4621 cgtcgtgcca ccagacgtcg cgcccgggga ccatgtggca ggtgccaccg gtgcgctgga
     4681 aaacgatcac ggtcttgacc gactcgcaac cgcccagcgc gatgccgtca tcgacggccg
     4741 gcttgagcgg aatcttcttg ccgccacggc attgctcgtc ggcggtaatc accgccacgg
     4801 cacccgcgtc attgacccgg tcacgtaccg actgggccga gaagccgcca aacacgaccg
     4861 agtggatggc accgatgcgc gcgcaggcct gcatcgcgat gatgccctcg acgcccatcg
     4921 acatgtagat caccacccgg tcgcccttct tgatgccacg tgcccgcagg ccattggcaa
     4981 actggcttac ccgcgacagc aattccttgt aggtgacttt cttgacctcg ccgtcatcgg
     5041 cttcaaaaac gattgccacc ttgtcgccga gtccggcctc gacgttacgg tccaggcagt
     5101 tgtatgaaac gttcagcgtg ccgtccgcga accacttgaa gaagggtgcg ttggattcgt
     5161 cgagtacctg cgtaaacggg gtcttccagg acacgtgctc gcgcgccagc cgggcccagt
     5221 aaccggtgta atccttttcc gcctcggcgc acagcgccct gtacgcatcc atgcccgaaa
     5281 tcgtggcctg cttgacgaat tcggcagaag gctcaaaaaa atttacggcc tcgttcagtg
     5341 acatgtccca tctcctgttg gttttggtgg aggattcaag aattcaatgc agcaagtcgc
     5401 gaaaagaacc catgcttcgc aacctccgcg tagcggaagg cagataatac gctgctcatc
     5461 cggccgcgtg tcccgccaat cgcttcagat taaacgccgc caatcttaca ggccacttac
     5521 gccagggcct tgcagcacaa ccgaaagacg cctttttttg atgctagaat cgtgtttttg
     5581 gatcattccg cactgaaaac caatggcctc actgaaaccg acggttgaca aactcgagat
     5641 gttcatcttc tggagccgct ggctgcaggc accgctctac cttggcctga tcattgccca
     5701 gggcgtctat gtctacctgt tcatgcttga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn cggagcacac gatcatgtgg caggtcatca tccatgcgct ttttctttgc
     5881 tcggcactgg cgatggtctg ggtagaccgg gtgatgaccc agacgctggc gctgagcaag
     5941 gcacagcacg ctgcgccgca ttgaaccgac acccgcggcg atgcctcgcc gccaccaacg
     6001 agagagcctg aaatgacgac tatcaagcag gaagacttca tccagagcgt tgccgacgcg
     6061 ttccagtaca tcagctacta ccatccgctc gattacatcc tggcgctcgg cgccgcctac
     6121 gaacgcgagc agagcccggc ggccagggat gccatcgccc agatcctgac caacagtcgc
     6181 atgtgtgcgg agggccaccg tccgatctgc caggacacgg gtatcgcggt gatctttctc
     6241 aaggtcggca tggacgtcca ttgggaggcc ggcctgtcgg tccaggaaat gtgtgacgaa
     6301 ggtgtccggc gcgcctacaa caacccggac aacaagctgc gcgcgtcggt gctgctggac
     6361 ccggccggcg cacgccgcaa ttcgcgcgac aacacccccg cggtggtgca ttacgagatc
     6421 gtcccgggtc accacctcga agtgatctgc gcagcgaagg gtggcggttc ggaaaacaag
     6481 tccaagttct atgcgctcaa tccctcggac tcgatcgtcg attgggtgct gaagaccgtg
     6541 ccgaccatgg gtgccggttg gtgtccgccg gggatcctcg gcatcggcat cggcggcacg
     6601 ccggaaaagg cgatgttgct ggccaaggag tcgctgatgg cgccggttga cattcatgaa
     6661 ttgatcgcgc gcggcccgaa gaatcgcgtc gaggagcttc gccttgagct ctatgagaaa
     6721 gtcaacgccc tcggtatcgg tgcgcagggc ctgggtggcc tgacgacagt gctcgacgtc
     6781 aagattcttg actacccgac gcatgcggca agcctgccgg tggcgatgat cccgaactgt
     6841 gcagcgacgc ggcacgcaca ctttcacctc gacggttccg ggccggcggt gctgacccct
     6901 cccgacctga atgactggcc caaggtcaac tggacgccaa acaccgaaac ctcgacgcgc
     6961 gtgaacctgg acacgctgac cgcggcggaa gtcgcggcct ggaaaccggg ccagacgctg
     7021 cttctcaacg gcaagatgct gaccggtcgc gatgcggcgc acaa
//
LOCUS       DAOSIT010000037         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9679_length_7063_cov_7.950771, whole genome shotgun sequence.
ACCESSION   DAOSIT010000037 DAOSIT010000000
VERSION     DAOSIT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9679_length_7063_cov_7.950771"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLL14_01775"
     CDS             complement(<1..611)
                     /locus_tag="PLL14_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG02676.1"
                     /translation="MKTVALLFALLLPAFASAGVYEDMEEALISGDTPWAIQLINRGM
                     DVNSVDAAGNTLLMQTVQRDNMELFDYLLKRRARINVRNRNGETALSLASYQGRLPFV
                     KSLVEAGADVNLYGWPPLIYAAFNGHAAVVDYLLKKGAEVNATTANGSTALLFAARFG
                     HIEVVKLLLQNRADPNIANERGATAIDWALKTDNTDIADLLRKA"
     gene            complement(608..1390)
                     /locus_tag="PLL14_01780"
     CDS             complement(608..1390)
                     /locus_tag="PLL14_01780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG02677.1"
                     /translation="MLVDSHCHLDFPELSARINQVLDLMHDNGVGCAVCIAVNLEELP
                     RVLALAETWPAILATVGVHPEATHVREPTVDELVDLARHPRVIAIGETGLDYYWHKDA
                     PEWQRERFRTHIRAARLAGKPLVVHTREAATDTLRVMQEEGASEAGGIMHCFTESWQV
                     AEAALDQGFYISMSGIATFKNAGTVHDVARRVPLDRLLIETDAPYLAPVPYRGKTNQP
                     GYVKHVAEEVARLRGLSYEHLVDATTTNFFRLFPEARLKVAL"
     gene            complement(1401..1781)
                     /locus_tag="PLL14_01785"
     CDS             complement(1401..1781)
                     /locus_tag="PLL14_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG02678.1"
                     /translation="MADAAPPKSSASTAPRPSVLSLNINSKSALYAAYMPLLRNGGIF
                     IPTTRNYAMGDEVFMLLSLMDDPAKLPIAGTVVWITPVGAQNNKAQGIGVHFKSDESG
                     VEARRRIEGLLGGVMQSGRPTHTV"
     gene            complement(1789..>2879)
                     /gene="holB"
                     /locus_tag="PLL14_01790"
     CDS             complement(1789..>2879)
                     /gene="holB"
                     /locus_tag="PLL14_01790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOG02679.1"
                     /translation="GHHRESDSKSCCRSLFMKIIEMHHVVWQQLCARRQQLPHALLIS
                     GPRGIGKFDLARAFAESLLCENLTDSGEACGKCLACGWLAQGNHPDLRRLQPEVVAAD
                     AGGAVAEGSDAGKKKPSQQITIDQVRGLDDFLHVGTHRHGARLVLLNPAEAMNRPTAN
                     ALLKSLEEPIDNTLFILVTSEPSRLLPTIRSRCQAVPVRCPARALAGRWLSDAGVRGA
                     GDWLALAGGAPLLALELSVSGERVLLDSLLAQVSRGTGLDALAAAANLDKTVRAEKRP
                     AALKRVIDWAQKWLFDLTLASEGLPPRYFLPQGPLLQRLAQATDTRKLLAFNKKALQY
                     RAQCEQPLNSRLFLEDFFLSYVRLFRTS"
     assembly_gap    2880..2979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2980..3310)
                     /gene="tmk"
                     /locus_tag="PLL14_01795"
     CDS             complement(<2980..3310)
                     /gene="tmk"
                     /locus_tag="PLL14_01795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG02680.1"
                     /translation="MQPGRFVTFEGIDGAGKSSHIATAAAFVRDHGKTVVCTREPGGT
                     RLGEKLRELLLHEAMHLETEALLMFAARREHLEQVIEPALARGDWVICDRFSDATYAY
                     QSGGRGLD"
     gene            complement(3325..4326)
                     /gene="mltG"
                     /locus_tag="PLL14_01800"
     CDS             complement(3325..4326)
                     /gene="mltG"
                     /locus_tag="PLL14_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG02681.1"
                     /translation="MRKLLRSLLVFFTLALTLLAVLVAALALTPLDLQRESIEFTVVP
                     GSSLRGAAREISAAGVELDPWILLLLARLMRVDASIKAGSYEISRGITPVDLLKKLTR
                     GDFTQAELVFIEGWTFSQLRRQLDAHPDLRHDTLGMPEAEIMRRVGASQGVAEGAFFP
                     DTYLFAKRSSDVDLLARAYRAMQRHLLDEWQGRAAMLPYRDAYQALIMASIVEKETGR
                     SEDRSLVAGVFVNRLRRGMLLQTDPSVIYGLGETFDGNLRKRDLLADTPYNTYTRPGL
                     PPTPIAMPGLASLQAAMHPAPTEALYFVARGDGSSHFSSTLAEHNQAVARYQRGAKP"
     gene            4534..>4932
                     /locus_tag="PLL14_01805"
     CDS             4534..>4932
                     /locus_tag="PLL14_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOG02682.1"
                     /translation="MSSSWLEFLGTCGARTAQGVVTDFGDAAAELAAARTATIVSPLR
                     HLGVLEVSGADAMSFLHQQLTSDVKHLAEDAAQHSAWCSAKGRMLASFLVYRKGPAYH
                     LELSADLLPRVRKRLQMFVLRSKVHIADLSD"
     assembly_gap    4933..5032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5033..5404
                     /locus_tag="PLL14_01810"
     CDS             <5033..5404
                     /locus_tag="PLL14_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOG02683.1"
                     /translation="IPLITERTQEEFVPQMANFEKLGAVSFHKGCYPGQEIVARTQYL
                     GKVKRHLYRVHSASPLAAGQALCSSANPEHPCGMIVNAAPAPAGGYDALAIVQEAFAA
                     AGDLELAVPGGARVAILPPGA"
     gene            5428..6210
                     /locus_tag="PLL14_01815"
     CDS             5428..6210
                     /locus_tag="PLL14_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOG02684.1"
                     /translation="MCLILIAWQAHPDYPLVVAANRDEFFARPTAAAAFWPDAPQVLA
                     GRDLEAGGTWLGVSRQQRFAAVTNYREGGRQRVDARSRGALVADFLTSQASPVAYLTP
                     LEADAAEYNGFNLVVGNGESLAYYSNRGDGQPRWLKPGLYGLSNHLLDTPWPKLASAK
                     AAFAAALLALPVQDPFLDLLADQEIVPDTHLPETGVPLAWERILSAVFVRSENYGTRA
                     STLLTIDRGGRTTLIERSFDAAARASGEVCETFQSSLISTVV"
     gene            complement(6180..6662)
                     /locus_tag="PLL14_01820"
     CDS             complement(6180..6662)
                     /locus_tag="PLL14_01820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOG02685.1"
                     /translation="MHIDISIKQQTLRLSDASGQVLRTYSVSTAAKGAGEVNGSYCTP
                     RGRHLIRAKIGAGQAENTVFVGRRPNGEIYSQDLARRFPKRDWILTRILWLSGCEPGR
                     NRLGRVDTMRRYVYIHGSPDSTPMGCPGSIGCIRMRNADIVELFERVPPYTTVDISED
                     "
     gene            complement(6665..>7063)
                     /gene="tadA"
                     /locus_tag="PLL14_01825"
     CDS             complement(6665..>7063)
                     /gene="tadA"
                     /locus_tag="PLL14_01825"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOG02686.1"
                     /translation="EVPVGALVVRDGVIVGRGFNSPIGEHDPTAHAEIAALRDAARNL
                     RNYRLPGCELFVTLEPCAMCAGAVLQARIARLIYGARDPKTGVHGSVVDLFAVERLNH
                     HTEVVGGVLAEACGQLLSDFFAARRSRRGE"
BASE COUNT         1260 a         2197 c         2143 g         1263 t
ORIGIN      
        1 gccttgcgca acaagtcggc aatgtcggtg ttgtctgttt tcagtgccca gtcgatggcc
       61 gtcgcgccac gctcgttggc aatgttcggg tcggcccggt tctgcagcag cagtttgacg
      121 acctcgatgt ggccaaagcg tgcggcaaac agcagcgctg tcgaaccatt ggccgtcgtc
      181 gcgttgactt cggcgccttt cttgagcagg tagtccacca ccgcggcatg gccattgaag
      241 gcggcgtaga tcagcggtgg ccagccatac aggttgacgt cggcgcccgc ttcgaccagg
      301 cttttgacga acggcagccg gccctggtag gaggccaggc tcagcgctgt ttcgccgttg
      361 cgattgcgga cattgatgcg cgctcgccgc ttcagcaggt agtcgaacaa ctccatgttg
      421 tcgcgctgta cggtttgcat cagcaaggtg ttgcccgcgg catcgaccga attgacgtcc
      481 atgccgcggt tgatgagttg gatggcccag ggcgtgtccc cggagatcag tgcctcctcc
      541 atgtcttcat acacgccggc ggaggcaaag gctggcagga gcaaggcaaa cagcagagca
      601 acagtcttca tagggcaact ttcaagcggg cttccggaaa cagacgaaaa aaattggtgg
      661 tggtggcatc gacaaggtgt tcatagctca ggccgcgcag gcgtgccacc tcctcagcga
      721 catgcttcac gtaacccggc tggttggttt tcccacggta gggtaccggc gcgaggtagg
      781 gggcgtcggt ttcaatcagt agacggtcga gtggtacacg ccgcgccacg tcgtgcaccg
      841 tgccggcatt cttgaaggtg gcaatgccgg acatggaaat gtagaaaccc tgatcgaggg
      901 ccgcctcggc tacctgccag ctctccgtaa agcaatgcat gatgccgcct gcttcgctgg
      961 cgccctcctc ctgcatcact cgcagcgtgt cggtcgcggc ctcgcgggta tgcaccacca
     1021 atggcttgcc ggcgagacgg gcagcacgaa tatgggtacg aaaacgctcg cgctgccatt
     1081 ccggagcgtc cttgtgccag taatagtcga gcccggtttc gccgatggca atcacgcgtg
     1141 ggtggcgcgc caaatcgacg agttcgtcga ccgtcggttc gcgtacatgg gttgcctcgg
     1201 gatggacgcc aaccgtagcc agaatcgccg gccaggtctc ggcaagcgcc agcacgcgcg
     1261 gcagttcttc aaggttgacg gcaatacaga cggcgcagcc cacgccattg tcgtgcatca
     1321 ggtcgagcac ctgattgatc cgggcggaca actcggggaa gtcgaggtgg cagtgcgaat
     1381 cgaccagcat cacggctcca tcagacggta tgcgtcggac ggccggactg catcacgccg
     1441 cccagcagcc cttcgatgcg ccgacgcgct tcgacgccac tttcgtcact cttgaaatgc
     1501 acaccaatgc cctgcgcctt gttgttctgc gcgccaaccg gggtgatcca gacgaccgta
     1561 ccggcaatcg ggagcttggc cgggtcgtcc atcagcgaca ggagcatgaa cacctcatcg
     1621 cccatggcat agttgcgggt cgtcggaatg aagatgccac cgttgcgcag cagcggcatg
     1681 tacgccgcgt acagcgcaga tttcgagttg atgttcagcg acagtacgct tgggcgcggt
     1741 gctgtgcttg ctgaagattt gggtggggct gcgtctgcca tatccctctc aggaggttcg
     1801 gaagagcctg acgtagctca ggaagaagtc ttcgagaaac agacggctat tcaatggttg
     1861 ttcgcactgg gcccggtatt gtagcgcctt tttgttgaac gccaagagct tgcgggtatc
     1921 ggtggcctgt gccagccgtt gcagcagtgg tccctgcggc aggaagtaac gcggtggcag
     1981 tccttcactg gccaaagtca gatcgaacag ccacttctgt gcccagtcga tgacccgttt
     2041 cagagctgcc ggacgctttt cggccctgac tgtcttgtcg agatttgccg ccgcagccag
     2101 ggcatcgagc ccggtgccac gcgacacctg cgccagcagc gaatcaagca ggaccctttc
     2161 gccgctaacg ctgagttcca gtgccagcag cggtgccccc ccggcgagtg ccaaccagtc
     2221 gccggcgccg cggacgccgg catcactgag ccagcgtccc gcaagggcgc gggccgggca
     2281 gcgcacggga accgcctggc aacggctacg aatcgtcggc agaaggcgtg atggttcact
     2341 ggtaaccaag ataaacaacg tgttgtctat cggttcttca agtgatttga gaagtgcgtt
     2401 cgccgtcggg cggttcatcg cctcggccgg gttcagcaat accaggcgcg cgccatggcg
     2461 atgggtgccc acgtgcagaa agtcatccag ccccctcacc tgatcaatcg tgatctgctg
     2521 gctgggcttc ttcttgcccg catcactgcc ttcggctacc gcgccccccg catcggcggc
     2581 cacgacttcc ggctgcaaac ggcgcaggtc cgggtggttg ccctgcgcga gccagccgca
     2641 agccagacac ttgccgcagg cctcaccaga atcggtgaga ttctcgcaga gcagcgattc
     2701 ggcgaaggcg cgtgccagat cgaacttccc gatcccgcgc gggccggata tcaggagggc
     2761 atgcgggagt tgctgacgcc tggcgcaaag ctgttgccaa accacatgat gcatctcaat
     2821 tattttcata aacaatgatc tacaacagct ttttgaatca gattctcgat gatgtccggn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcgaggccg cgtccgccgc
     3001 tttggtaagc ataggtggcg tcgctgaagc ggtcacagat cacccagtcg ccacgcgcca
     3061 gggccggctc gatcacctgc tcgagatgct ccctgcgggc ggcaaacatc agcagggcct
     3121 cggtctccag gtgcatggcc tcgtgcagca gcagttcgcg gagcttctcg ccaagccgcg
     3181 taccgccggg ctcgcgggtg cacaccaccg ttttgccgtg gtcgcgaaca aacgccgcgg
     3241 cagtggcaat atggctgctc ttgccggcgc cgtcgatgcc ttcaaaggtg acgaatcggc
     3301 cgggctgcat gcctgcaccg tgactcaagg tttcgcaccc ctctgataac gtgccacggc
     3361 ctggttgtgt tccgcgagcg tgctggaaaa atggctgctt ccatcgccgc gcgcgacgaa
     3421 gtacagcgcc tcggtcggag ccggatgcat cgccgcctgc agggatgcga ggccgggcat
     3481 ggcaatcggc gttggtggca ggccaggacg cgtgtaggta ttgtagggcg tatcggcgag
     3541 caggtcgcgc ttgcgcaggt tgccgtcgaa ggtttcaccc aggccgtaga tcacgctggg
     3601 gtcggtctgc aacagcattc cccggcgcaa ccggttgacg aatacgccgg ccaccagcga
     3661 acgatcctcc gaacgcccgg tttccttttc gacgatcgaa gccatgatca gggcctggta
     3721 ggcatcacga taaggcagca ttgccgcccg cccctgccac tcgtccagca gatgccgctg
     3781 catggcgcgg tacgctcgcg ccagcagatc gacatcactg ctgcgcttgg caaaaaggta
     3841 ggtatcggga aagaatgccc cctcggctac cccttgcgac gcgcccaccc tccgcatgat
     3901 ctccgcctcg ggcatgccca gcgtgtcgtg gcgaaggtcc ggatgcgcgt cgagttgccg
     3961 gcgcaactgg ctgaaggtcc agccttcgat gaaaacaagt tccgcctgcg tgaagtcgcc
     4021 gcgcgtcaac ttcttcagca ggtcgaccgg cgtgatgccg cgactgattt cgtagctacc
     4081 ggccttgatc gaagcgtcga cacgcatcag cctggccagc aggagcagga tccacgggtc
     4141 cagttcaaca cctgcggcgg agatctcccg tgcagcgccg cgcaggctgc tgccggggac
     4201 gacggtgaat tcgatcgact cccgctgcaa atccagtggc gtcaacgcca gagcggcaac
     4261 cagcacggcc agcagcgtca gcgcgagggt aaaaaaaacc agcaagctgc ggagaagttt
     4321 acgcatcaat ccggcgcagc cggggaggct gcagggcggt aacggggaat gggcttcgag
     4381 tcgtcctata atagccgaaa gccgcagccc tgaactcacg acgcacccgg agggtcagag
     4441 ctgacgcccg tcgggacctt catgaaaacc gctttgccgc ggtaggcgac gagtccacac
     4501 ttccgaaaac cacctgatga aaatgaagcg acaatgagtt cgagctggct ggaatttctt
     4561 ggcacctgtg gtgcacgcac ggcacagggc gtggtcaccg attttggcga tgccgctgcg
     4621 gaactcgccg cagcgcgtac ggcgaccatc gtctcaccgc tgcgccacct cggcgtactc
     4681 gaagtcagcg gcgccgacgc catgtcgttt ctgcaccagc aactgaccag cgacgtcaag
     4741 cacctcgccg aggatgcggc gcagcattcg gcctggtgct cggccaaagg ccggatgctc
     4801 gccagctttc ttgtctaccg caagggtccc gcctatcatc tggaattgtc cgcggacctg
     4861 ctgccgcggg tccgcaaacg cctgcagatg ttcgtcctgc gcagcaaggt gcatatcgcc
     4921 gacctgtcgg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattccact
     5041 gatcaccgaa cggacgcaag aagagttcgt tccgcaaatg gccaatttcg aaaaactggg
     5101 cgcggtcagc ttccacaagg ggtgttatcc cggtcaggaa atcgtcgccc gcacccagta
     5161 cctcggcaag gtgaagcgcc acctctatcg cgtgcactcg gccagcccgc tggccgccgg
     5221 tcaggcgctc tgttcatcgg ccaaccccga acatccgtgc ggcatgatcg tcaacgccgc
     5281 accggcaccg gcgggcggtt acgacgccct ggcgattgtc caggaggctt ttgccgctgc
     5341 cggtgatctg gaactcgccg ttccgggtgg cgcgcgtgtc gctatcctgc cgccgggggc
     5401 gtaggtcacc gcctgccgct gccgacgatg tgcctcatcc tgatcgcctg gcaggcacac
     5461 ccggactatc cgctggtcgt tgccgccaat cgcgacgaat tctttgcccg tcccaccgcc
     5521 gccgccgcgt tctggccgga cgccccacag gtcctggcgg gacgcgatct cgaagccggt
     5581 ggtacctggc taggcgtcag ccggcagcag cgcttcgccg ccgtgaccaa ttaccgcgag
     5641 ggtggccggc aacgtgtcga cgcccgctcg cgcggtgccc tggtcgctga ttttctgacc
     5701 agccaggcca gcccggtcgc ctatctgacc ccgctcgaag ccgacgctgc cgaatacaac
     5761 ggcttcaacc tcgtggtcgg taatggcgag tctctggcct actactcgaa ccgtggcgac
     5821 ggccagcccc gctggctgaa acccggcctc tatggcctct ccaatcatct cctcgacacg
     5881 ccgtggccga agctggccag cgccaaggcg gcctttgccg cggcgctgct ggcgctgccg
     5941 gtgcaggatc cgttcctgga tctgctcgcc gaccaggaaa tcgttcccga tacacatctg
     6001 cctgaaaccg gcgtaccgct ggcctgggag cgcatcctgt cggcggtgtt cgtgcgctcg
     6061 gagaactacg gcacgcgcgc ctcgacactg ctgaccatcg atcgcggtgg gcgaaccacg
     6121 ctgatcgagc gcagcttcga cgctgctgcc cgggcatccg gcgaagtctg cgaaaccttt
     6181 cagtcttcgc tgatatcgac tgtggtatag ggcggtaccc gttcgaaaag ttcgacgata
     6241 tcggcgttgc gcatgcggat gcagccgatc gacccggggc agcccatcgg cgtgctgtcc
     6301 gggctgccgt ggatgtaaac gtagcggcgc atggtatcga cgcgtccgag gcggttgcgc
     6361 cccggctcgc agccggatag ccagaggatg cgcgtgagaa tccagtcacg cttcgggaag
     6421 cgcctggcga ggtcctggga gtagatttcg ccgttcggcc ggcgtccgac aaacaccgtg
     6481 ttttcggctt gtcccgcgcc aatcttggca cggatcagat gtctgccgcg cggcgtgcaa
     6541 taactgccat tgacttcacc agcgcctttc gctgccgttg acacgctgta ggtgcgcagc
     6601 acctgaccgg aggcgtcgga caggcgcagc gtctgctgct tgatcgagat atcgatgtgc
     6661 accctcattc ccctcgacgg ctgcgccgtg cggcaaaaaa gtcggaaagc aactgcccgc
     6721 atgcttcggc caggacccca ccgacgactt cggtgtgatg gttgaggcgt tcaaccgcaa
     6781 aaagatcgac cacgctgcca tgcacgcccg tcttcggatc ccgggcaccg tagatcaggc
     6841 gggcaatgcg cgcctgcaag acggctccgg cacacatggc gcaaggttcg agagtcacga
     6901 acagttcgca gccgggcaga cggtagttcc gcaggttgcg cgcagcgtcg cgcaaggcgg
     6961 caatctcggc atgcgccgtc gggtcatgtt cgccgatcgg cgagttgaag ccgcgcccga
     7021 cgatcacccc atcacgcacg accagcgcac ccaccggcac ctc
//
LOCUS       DAOSIT010000038         7037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9727_length_7037_cov_8.022343, whole genome shotgun sequence.
ACCESSION   DAOSIT010000038 DAOSIT010000000
VERSION     DAOSIT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9727_length_7037_cov_8.022343"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLL14_01830"
     CDS             complement(<1..250)
                     /locus_tag="PLL14_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOG02687.1"
                     /translation="MTTLIPPDYAAWLAELKDAIRTARLKAGLAVNWELVLLYWRIGK
                     EILERQSAQGWGAKVIDQLARDLRAEFPDMRGLSPRNMK"
     gene            complement(261..899)
                     /locus_tag="PLL14_01835"
     CDS             complement(261..899)
                     /locus_tag="PLL14_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG02688.1"
                     /translation="MPLVTKRAAARRDLVEQFVYLAENAGLNVAERFLSPAEASFADL
                     ARQPMMGAPVTLKHPDLAGMRKWRVKDFDNHLVFYQPRSGGVSIVRVCMRPATGGACW
                     DSRRSGTENRAGSRLRRCLMALMLKDDILIRDAATEVSAFLSDLTPNRPGPPRSLCSS
                     ADRRDGRRSRSSRFVSCRLADLGRRAGSIHAELRNSAAAWWTIPDQVTRRKP"
     gene            complement(899..1159)
                     /locus_tag="PLL14_01840"
     CDS             complement(899..1159)
                     /locus_tag="PLL14_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOG02689.1"
                     /translation="MQSMNISLPDPLKKFVDGQIAQGRYSSVSEYVRELIRADEKRKA
                     EEQLEAKLLEGLSGAESELTPADWSAIRREALAKVEARKKPR"
     gene            1392..1710
                     /locus_tag="PLL14_01845"
                     /pseudo
     CDS             1392..1710
                     /locus_tag="PLL14_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611249.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            2282..2989
                     /locus_tag="PLL14_01850"
     CDS             2282..2989
                     /locus_tag="PLL14_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02690.1"
                     /translation="MSRHQEWKMVHLLYQYAHRSGRSARPVLTEDELRNAGVHCDVDT
                     WQILKDASVVKRVGNAFELSKAARQLVSTFTLAKGPETDQDIRVDYPEAFVVMPFSQP
                     WSNEVYENLFKPGIEDAGFEATRGDSIVRLGDLRTNVWRSITQAGVIVADVTTPNPNV
                     YYELGLADALGKATFLFKQEGSSLPADIGGEHFYEYNLKHLGANQQVLTQALRKWANE
                     RDNMFFGVKTLADAHHK"
     gene            3701..6040
                     /locus_tag="PLL14_01855"
     CDS             3701..6040
                     /locus_tag="PLL14_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02691.1"
                     /translation="MAQEKKGSRPGDGNSAPSKLAEALTHVVRELSPADREDVHDPDF
                     QFALRELLQAYKPLLEADLARVDAPDELTKEVLEAGPNCEDEFAAADQLLGRFLTEEV
                     AVRLLPANARELLGPIERWRWCLLHIRCCIIFGWLLCRRPHTFRTSNYYLYRFWRCVR
                     QAIGTPVGDALNAAERADFARLVQAMAAAYKPYLSDQLASAEFPSGIPDEIISGKLDC
                     LEDDDTAGSIFERLLAPDVAPALLGEEMFAKHQQEPWFWFCRCWCLCAIRFGCCLARI
                     RNLRDFVRCLLFYRRCLRQCFRPLSCELTEPTGCVPEEVNVELKALVVAVRGTAAGGM
                     FHHYVLEWSQDGINWHASDFHYPPIPPGGGAQGNFAVNNGLLAYFDTSARDPGLYFIQ
                     MTVFSVEGTTQVCPTRFSLFKQEVRIISVDGAALDTVWADPNARFIESVPGICEDPPG
                     TWRRFPSTDEMSFGECLHIYGGAFVGGCDGKRIKRYMIDYKPGFELDCFTAGWSNIWK
                     VEFNTVWQYRDSNMRKDTSNLTAVWNTDCIVPVFFPPYCLHSEPQARLDPSCWPSHTG
                     LCELSGLVTLRLMVEDTDGNLYCDTQRLWIDNKPICAKVRIDAVPKCQDLFVSQFANP
                     PECSAAWNLPLSGIAYDAYINDALPLTRPNDNFDYYQIHVTKQGGPTITIPISMGPTA
                     PCFKGTKRIGKCDPCNALDPHAGDVYGTLAQFDLRAVDLACKGSLPYAVPDAFTIPRQ
                     ECCVYTFDLWVYDRTVRSSGINWAHASWPVKICNDLKPT"
     gene            6095..6502
                     /locus_tag="PLL14_01860"
     CDS             6095..6502
                     /locus_tag="PLL14_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02692.1"
                     /translation="MTEIGFWPTLFLTTLAAWRLTHLLANEDGPADLVLRLRRALGDS
                     GIGHLMDCFQCLSLWVAAPLALILSTELLTWLIAWLGLSGAACLLERCGRQVEAIQAP
                     PLLIGGDIDHGMLWPRQGSAEEKPDGESAGGAR"
     gene            6606..6761
                     /locus_tag="PLL14_01865"
     CDS             6606..6761
                     /locus_tag="PLL14_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02693.1"
                     /translation="MLRYLEHSPVVIHGSSTGRRYEFSAASPKQRVDARDAESLLATP
                     FFRRVNA"
BASE COUNT         1391 a         2117 c         2090 g         1439 t
ORIGIN      
        1 acttcatgtt gcgaggggaa agcccgcgca tgtcggggaa ctcggcacgg aggtcacggg
       61 ccagttggtc gatgaccttc gccccccaac cctgggcgga ttgccgctcg agaatctcct
      121 tgccgatgcg ccaatagagc aggaccagtt cccagttaac cgcgagacca gctttgagcc
      181 tcgctgtgcg gatggcgtcc ttcaactccg ccagccatgc ggcgtagtcg ggagggatga
      241 gggttgtcat gcttcggcgg ttatggtttt cggcgagtca cttgatctgg aattgtccac
      301 caggctgctg cactgttgcg gagttcagcg tgaatcgacc cggcacgcct cccaaggtcg
      361 gccagccggc atgatacgaa gcgcgaagaa cggctgcgcc gtccatcgcg ccggtctgcc
      421 gatgaacaca gtgatcgggg tggcccggga cgatttgggg taaggtccga caaaaaagcg
      481 ctcacttccg ttgcggcgtc tcgaatgaga atgtcgtctt ttagcataag cgccatcagg
      541 caccttcgaa gccgcgatcc tgcacgattc tccgtcccgc tacgcctcga atcccaacag
      601 gctccaccag tcgctggccg catgcagaca cgcacgatgg acacgccgcc ggagcgcggc
      661 tggtagaaca ccaggtgatt gtcgaaatcc ttgacccgcc atttgcgcat gcccgccagg
      721 tcaggatgtt tgagcgttac cggtgcgccc atcatgggct gccgcgccag gtcggcgaag
      781 ctggcttcgg ccgggctcag gaagcgttcg gccacgttca gcccagcgtt ttcggccaga
      841 tagacgaact gttctaccag gtcgcgccgc gcggccgcgc gcttggtgac cagcggcatc
      901 agcgcggttt ctttcgcgct tcgaccttgg ccaaggcttc cctgcggatg gcgctccagt
      961 ccgccggggt cagttcactt tcagcgccgc tcagtccttc caacagcttc gcttcaagct
     1021 gttcttcggc tttgcgtttc tcgtcggcgc ggatcagctc gcgtacatat tcgctcacgc
     1081 tgctgtagcg gccttgggcg atctgtccat ccacaaactt ctttaagggg tcgggcagcg
     1141 aaatattcat gctttgcatg gagcacctcc ttatctcacc taaagtgcca caaacagcaa
     1201 aagatggcaa taactgccat gtgacccagg gcaatcgcat gaatgccatc gtcgtgaacc
     1261 ctccaaaata atcgtttacg ctcaatgagt tacaaagggg ctgttcacag ccccttgatt
     1321 tgtatagttt tctgtctttt tggggttttc catgagggca gaaagcaagt tccgattggt
     1381 cgacgtctgg gcacgtctat cactcgtgca ggaaccggca ctgcccgcta tgtcagaccc
     1441 gggcgaagga agcctgggtc gccgcaaggc ggcgtgaagt gctgcccgtg ccttactttc
     1501 acctggtgtt cacgctgccg cacgacctca atggcctgat tgccgccgca ccacggtcgc
     1561 ttcacgagac gctgttctcc gcggtatcgg caacgcttac cgaattcgcc gccaacccgc
     1621 gacatctggg cggcgtgtcg gcgttcttgc tggtgctgca taacgtggaa gcaggatctc
     1681 ggccgacatg tccatctgca tgcgctggta cttcaagacc cgaggcaccc gaagctgggt
     1741 atttgccgca acagaagaaa aagaggatgg aacgaaaagg gaacttacct tgctgctaga
     1801 gtcggatacg ccgataagac ggcatgtcaa gatcaaagct gacgcgaacc cgcatgaccg
     1861 ccaatgggaa cagtacttcg aggcccgatg gggcaagaaa atgctgtatt cagtgtgcgg
     1921 tcgcaggaag ttttaccgcg tctggcaaag gcaagacggc ctgtgttcgg tctgtcaaaa
     1981 ttccatcacg acgagcactc cctggggtgt tcgccacatc gtgaaacgga cggatggcgg
     2041 atcggatgcc gcaagcaacc ttcagatgca acatctcagt tgccgtagaa acctgtagta
     2101 tgcccgaatg aaagttgtga aaccgggtat cgaaagatgc ctttgtagag gcttgagccg
     2161 tgtgtgctga aaggtgcaag cacggttctt agggggccgg gcgcgggcaa ccgtgttcgg
     2221 ctacccgacg ttaggcggca taacccatca ccgtgaatcg tctacctaaa aggagatcga
     2281 gatgagccga caccaggagt ggaagatggt tcacctgctc tatcagtacg ctcatcgcag
     2341 cgggcgcagt gctcgcccgg ttctaaccga agacgaactc cggaacgctg gagtccactg
     2401 cgacgttgac acgtggcaaa ttctgaaaga tgccagcgtg gtaaagcggg ttggaaacgc
     2461 cttcgaactc tctaaggccg ccaggcagct cgtaagcaca tttacccttg ctaagggtcc
     2521 tgaaaccgat caagacataa gggtcgacta cccagaagca tttgtcgtca tgcccttcag
     2581 tcagccatgg tccaacgagg tatacgagaa tctgttcaaa cctggaatcg aggatgctgg
     2641 ctttgaagcc actcgtggcg actccatagt acgcttgggt gacctccgga caaatgtttg
     2701 gaggagcatt acgcaggcag gagtcatcgt tgctgatgtc acaactccaa atccaaatgt
     2761 gtactacgaa ctggggctag cagatgcgct cggaaaggca acctttctct ttaagcagga
     2821 gggttcctcc ttaccagcgg acatcggcgg cgagcacttc tacgagtaca acttgaagca
     2881 tcttggcgcc aatcagcagg tgctcaccca ggccctccga aagtgggcca atgaaaggga
     2941 caacatgttc ttcggtgtca agacgcttgc tgacgctcat cacaagtgaa aatgccgcct
     3001 accattcgct caaccggagc cattgcggca tgcggctaaa tgcccgccat ttcattctga
     3061 gcctttagcc acacaccgca acggtccggt tagctcgaac gctgaacgtc cgctatccta
     3121 atcgggctac ttccgctttg ggtcgtaacc ggaagctcac gattcgaagg gactgcggtc
     3181 gtggcgagct ttgctggctg gaaaagctga accccgcaaa tgacataggc ctaacccggt
     3241 tttccgttcc actgcgcctc aaatcccaag tgcgtcgcga gcgggctggc cgcggtcccg
     3301 agaccaagct gtgacggttg tcgttgccga tacgcgcgtc tatgtttcgg cgctcgtctt
     3361 cggtggtgtg ccgttggccg ccctcactaa aacgctcacg gcccccagac tgtccaataa
     3421 gcggcttttt ctgtcagaac gatatgctct cgttggtccg gttggatttc ctatccttca
     3481 gacctaaaag taagcagctg acatggcgca ccggatactc ctgccaatcg ggggggtgta
     3541 gggcttttcc acccactggg tgccgagcac caccgcagga tccttccaat cgccagcagc
     3601 tttgtcctaa ccccggtttt ggccatggcc tacactttca gcgacggggc cggctgccgt
     3661 cgggtagcgg tgaacgcttg gtgtggaaca ggaggctgac atggcacaag agaaaaaagg
     3721 tagtcgtccg ggtgacggca acagcgcacc gtccaagctg gcggaagcgc tgacgcatgt
     3781 ggtcagggaa ctcagtcccg cagataggga ggatgtccat gatcccgatt tccagttcgc
     3841 cttgcgcgag ctgctgcagg cttacaagcc gcttttggag gccgatctgg cgcgagtgga
     3901 cgcgcctgac gagctgacca aggaagtgct cgaagcgggg cccaattgtg aagatgagtt
     3961 tgccgccgcc gaccaactgc tcggcagatt ccttaccgaa gaggtggccg ttcgcctttt
     4021 gccggcgaat gcgcgcgaac tgctcgggcc gatcgagcgc tggcggtggt gcctgctgca
     4081 tattcgctgc tgcatcatct tcggctggct gttgtgtcgc cgaccgcaca ccttccggac
     4141 gtccaactac tacctgtacc gcttctggcg ctgcgttcgc caggcgattg gcacgccggt
     4201 cggcgatgcg ctgaatgctg ccgagcgcgc cgacttcgcg cggctggtgc aggcaatggc
     4261 ggcggcctac aagccctacc tctccgatca actggccagc gccgaattcc cgagcggaat
     4321 tcccgacgaa atcattagcg gcaagcttga ctgtctggaa gacgacgata ccgccggctc
     4381 cattttcgag cgcctgctgg cccccgacgt ggcgcctgcc ttgctgggcg aagagatgtt
     4441 tgccaagcac cagcaagagc cgtggttctg gttctgccgc tgctggtgtc tgtgcgcgat
     4501 ccgcttcggc tgttgcctgg cgcggattcg caacctgcgc gatttcgtcc gctgtctgct
     4561 cttctatcgg cgttgcctgc gacagtgctt ccgcccgctg agctgcgagt tgaccgagcc
     4621 gaccggctgc gtccctgaag aggtcaatgt cgagctcaag gcactcgtcg tcgccgtccg
     4681 tggaacggcg gccggtggca tgttccacca ctatgtgctc gaatggtcgc aggacgggat
     4741 caactggcat gcgtccgatt tccactatcc gccgattcca cccggcgggg gcgcgcaggg
     4801 gaacttcgct gtcaataacg ggttgttggc ctatttcgac accagcgcgc gcgatcccgg
     4861 cttgtacttc atccaaatga ccgtcttctc ggtcgagggc acaacccagg tttgtccgac
     4921 gcggttctcc ctgttcaagc aggaagtacg aatcatcagc gtggacggcg ccgcgctcga
     4981 cacggtctgg gccgatccga acgcgcgatt catcgagagc gtgccgggca tctgcgaaga
     5041 cccaccaggc acttggcgac gattcccgag cactgatgag atgtcgttcg gcgaatgcct
     5101 gcacatctac ggcggcgcct tcgtcggggg ttgcgacggc aagcggatca agcgctacat
     5161 gatcgattac aagccggggt tcgaactcga ttgcttcacc gccgggtgga gcaacatctg
     5221 gaaggtcgag ttcaacacgg tctggcagta ccgcgattcg aacatgcgca aggatacgtc
     5281 caacctgacc gccgtctgga ataccgactg cattgtgccg gtgttcttcc cgccgtactg
     5341 cctgcactcg gagccccagg cgcgcctcga tccatcgtgc tggccaagcc acaccgggct
     5401 ctgcgaactg agcgggttgg tcaccttgcg cctgatggtc gaggataccg atggcaacct
     5461 ttactgcgac acccagcggc tgtggatcga caacaagccg atctgcgcca aagttcgcat
     5521 cgatgccgtg ccgaagtgcc aggatctgtt tgtcagccag ttcgccaatc cgcccgaatg
     5581 ctccgcggca tggaacctgc cgctgtccgg cattgcctac gacgcgtata tcaacgatgc
     5641 actgccgcta acgcgcccga acgacaactt cgactactac cagatccacg tcaccaaaca
     5701 gggcgggccc acgatcacca tccctatttc catggggcca actgcaccgt gtttcaaggg
     5761 caccaagcgc atcgggaagt gcgatccatg caatgcgctc gatccgcacg ccggcgacgt
     5821 ctacggtacg ctggctcagt tcgatctgcg ggcggtggac ctcgcctgca agggcagcct
     5881 gccctacgcg gtaccggacg ccttcacgat cccgcgccag gaatgctgcg tctacacctt
     5941 cgacctgtgg gtttacgacc gtactgtgcg ctcttcgggc atcaactggg cgcacgcctc
     6001 gtggccggtg aagatctgca acgacctgaa gcccacctga cttgaagcac acaaggggcc
     6061 ggtccgtaac cgacccctcc ccggggacgc agcgatgacc gagatcggat tctggccgac
     6121 attgtttttg actacactcg cggcgtggcg actgacccac ctgcttgcca acgaggacgg
     6181 gccggcggac ttggtacttc gcttgcggag agcgctgggg gacagcggca tcggtcatct
     6241 gatggattgt tttcagtgtc tcagcctgtg ggtcgcggca ccgctggcac tgatcctcag
     6301 tacggaatta ctcacctggc tgatcgcctg gctcgggctg tcgggcgcgg cctgcctgct
     6361 cgaacgctgt gggcggcagg ttgaggcaat ccaggcacca ccactcttaa tcggcggaga
     6421 tatcgatcat gggatgctgt ggccaaggca gggaagcgct gaagaaaagc cagacggcga
     6481 gtccgccggt ggcgcacgtt agcgccgcga cgtcgtccgt ttatcaacga tctggcggcg
     6541 cacgcccggc cgcatccgct attggcgcgg cggcaagcgg tgctgctggc gcgtcgggac
     6601 aggtcgtgct gcgctacctg gagcactcgc cggtggtgat ccacggcagt tcgactggcc
     6661 ggcgttacga attctccgcg gcctcgccga agcagcgagt cgacgcgcgt gacgcggagt
     6721 cgttgctcgc aacgccgttc ttccgccggg taaacgcttg accgcatggc catgagtcgg
     6781 cgatcggcaa agccgtcgtt tacccacggg caagagccag gttgatcaat gacttttccc
     6841 aagtgttgcc tataatataa cgaagctttt ttgcattcgg cccgaaaaca tcgtcattcc
     6901 tggactggac caatagtccc cggatctgca ggtcctgcca cccccaaggg ttcgcaagag
     6961 cagggcctcg gagtgacatc gagcgggagg tgaccgtgcc aacggaacgt gaaatccaga
     7021 aggttcaacg gcgcgtg
//
LOCUS       DAOSIT010000039         7031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9740_length_7031_cov_9.884604, whole genome shotgun sequence.
ACCESSION   DAOSIT010000039 DAOSIT010000000
VERSION     DAOSIT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9740_length_7031_cov_9.884604"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1453
                     /locus_tag="PLL14_01870"
     CDS             26..1453
                     /locus_tag="PLL14_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG02694.1"
                     /translation="MIRRLRAVLPVQALLVEEEDLRPYDCDGLTAYRQLPLLVALPEN
                     ESQAQAILKVCHELKAPVVARGAATGLSGGATPHADGVVVSLARLKKIVSIDALSRTA
                     VVQPGVRNLAVSEAAAPYGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTVHNVL
                     RVRGLTMAGEIVEFGNAALDAAGYDLLALLNGSEGLLAMITEVTVRLTPKPQLAQVVI
                     ASFDDVRRAGDAVAGIIAAGIIPAGLEMMDRKAIHAVEPYVNAGYDMDAEAILLCESD
                     GTPEEVAEEIGRMTAVLERSGASAYCVSRTEAERLKFWAGRKAAFPAVGRLTPDYLCM
                     DGTIPRKRLAEMLQEITVLSGKYDLRCANVFHAGDGNLHPLIMYDANQPGQLERAMAF
                     GAEILELSVRFGGTITGEHGVGIEKVNLMAEQFGMAEIETFHRLKAAFDEHGLLNPGK
                     GVPTLARCREYIQARVARAPTAPRF"
     gene            1453..2514
                     /gene="glcE"
                     /locus_tag="PLL14_01875"
     CDS             1453..2514
                     /gene="glcE"
                     /locus_tag="PLL14_01875"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HOG02695.1"
                     /translation="METLIDDWRARIAAAAGSGGKLAIRGGGSKCFYGGPETGERLDV
                     EAYRGIVAYEPSELVVTARAGTGLRELAATLAAKGQGLVFEPPHFGAAATLGGMLATG
                     LSGPRRQAVGAVRDFVLGVKLLNGSGEVLSFGGQVMKNVAGYDVPRLLAGSLGTLGVV
                     LEVSLKVLPLPVAEKSLRFALDEAAALQKLNQWGGRPLPISASAWHDGLLTLRLSGAA
                     AAVAAAERELAGEVLADADAFWDSLREQKQAFFLGDAPLWRLSLPSVTPPQALGETLI
                     EWGGAQRWLRGGDARRIRAAAAKAGGHATLFRGDDALKTEVGVFQPLTAPLARIHRHL
                     KQAFDPQGVFNPGRMYPGL"
     gene            2528..3781
                     /gene="glcF"
                     /locus_tag="PLL14_01880"
     CDS             2528..3781
                     /gene="glcF"
                     /locus_tag="PLL14_01880"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HOG02696.1"
                     /translation="MQTNLADFIKDTPVGREADAILRSCVHCGFCNATCPTFQLLGDE
                     LDGPRGRIYLMKQMLEGHAVTTRTQLHLDRCLNCRACETTCPSGVRYHRLLEIGRAVI
                     EQRVPRSPAARATRFLLCEALPRNWIFKPAVRVGQLLRPLLPAALADKVPSAAGGVRR
                     PWPRPAARPRRMIALAGCVQPTLTPNTNAAMARVLDRLGIELCEVPGAGCCGALRYHL
                     NAQEKALDDMRRVIDAWWPHVENGSVEGFVMTASGCGAHVREYGELLRHDPAYADKAA
                     RIAAMTRDASEVLAAEQQQLVELLRRQGDGSGQRVAFHSPCTLQHAQKIRGVVEALLT
                     AAGYQLSPVADSHLCCGSAGTYSISQPILARQLRDNKLAAMRAGSPALLVTANIGCQT
                     HLQSGSKIPVRHWIELIDERLGAAS"
     gene            3802..5445
                     /locus_tag="PLL14_01885"
     CDS             3802..5445
                     /locus_tag="PLL14_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG02697.1"
                     /translation="MELDSQAGSYQELCRSFRWQIPEQFNLAVDICGRWAEQRHRFAL
                     YYEDESGFTSAHTFWDIQRQANRLSNVLAALGTLPGDRVAILLPQCPEAAIAQIAICQ
                     MGALAVPLSPFLGPDSLEHRLGDAAAHLAIVDATTQPGVLGLRQRLPRLRHVLGVGSA
                     AGPGIRPWDEVLEHASARYTPTVTAAGDPAMILYRRGTQGGTRGALMAHRMLFGKLSA
                     FVCAHDFFPQPRDMLWSPGEPAWAGGFWDALLPTWHFGMPLLAYNGPLDAVKVFALIE
                     KYGIRNAVLWPTLLQRMMQRVADPGAVYDLDLRTLASVGEPVDPALWHWARDKMGVAI
                     NGVFGPAGLPDLVGNWASRWPASPGAVGRAYPGHRVAVIDAQGNALPPGEVGELAVFR
                     QYHAEDDPLPMLGYWRDPEATSEAVVGDGWVRTGESATLDGEGNLFCQRPADDTFASA
                     GYRVDPSEIETCLRAHADVANCAVIGRADELGGTRPRAFVVLRPGVLGTPELVRELQR
                     HVGRYLAPYETPKAIEFTAALPLGASGEVLRCALSTREA"
     gene            5484..5786
                     /locus_tag="PLL14_01890"
     CDS             5484..5786
                     /locus_tag="PLL14_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02698.1"
                     /translation="MSEQLEQAAGLVDERRQNQRSRAAFDSAFVMIEPFFDPGQGWGG
                     QSLELLAYRIVRENFPELSGEEVHAVVVAAHRVYIERNPQRSDHLPRPSELRRVTL"
     gene            5874..6689
                     /locus_tag="PLL14_01895"
     CDS             5874..6689
                     /locus_tag="PLL14_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOG02699.1"
                     /translation="MPISIDACVAQHQGDRREQQDRVAILPHPRGGGVALAVLADGMG
                     GHTGGVIAAQQVIHTARNNLEGYSARTESARVLLESCINEAHMLIKASRFINEKDPHS
                     TVVMLLLQPGRVSWAHCGDSRFYHFRGDALLFRSTDHSYVAQLVVQGRITPEQALVHP
                     NRNILLTSLGGGDAPKIAFGEAEGLQAGDTFLLCSDGLWGYFTDQELAWVISGSSSAR
                     EASELLIGRARSMGHGDGDNISVAILKLVNVPQPEPAATPGARTTLLAPEAAK"
     gene            complement(6663..>7031)
                     /locus_tag="PLL14_01900"
     CDS             complement(6663..>7031)
                     /locus_tag="PLL14_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOG02700.1"
                     /translation="EQVAIPGFEPEYDYCYPPGDKRSHHRRPTAAPPALAPLATAPGE
                     RAPSRAAAREGSNRPGSGRRPSHIAADGFDFSKPYESPEPHPVAAAVGSEAERALARS
                     EHPHKPRRLVAALLGGLGRK"
BASE COUNT         1104 a         2200 c         2472 g         1255 t
ORIGIN      
        1 gcgtttcgcg gttgaccggc cgaccctgat caggcgtttg cgcgcggtgt tgccggtgca
       61 ggccctgctg gtcgaggaag aggacctgcg gccgtatgat tgcgacggac tgaccgctta
      121 tcgccagttg ccgttgctcg tcgcccttcc cgagaacgag tcacaggcac aggcgatcct
      181 caaggtctgc catgagctga aggccccggt ggtggcgcgc ggtgccgcca ccgggctgtc
      241 gggcggcgcg accccgcatg ccgacggcgt tgtggtgtcg ctcgccaggc tcaagaagat
      301 cgtcagtatc gacgccctgt cgcgtaccgc ggtggtgcag ccgggggtgc gcaacctggc
      361 ggtgtccgag gcggcggcgc cctatgggct gtattacgcg cccgaccctt cgtcgcagat
      421 cgcctgtacg atcggtggca acgtcgccga gaactccggc ggtgtgcact gcctcaagta
      481 tggcctgacc gtgcacaatg tcctgcgtgt gcgcggcctg accatggcgg gtgagatcgt
      541 cgagttcggc aacgcggcac tcgacgcggc cggctacgat ctgctggcgc tgctcaacgg
      601 ctccgaaggt ctgctggcca tgatcaccga ggtgaccgtc aggttgacgc ccaagccgca
      661 actggcgcag gtggtgatcg cctctttcga cgacgtgcgg cgagccggcg atgccgtcgc
      721 cgggatcatt gccgccggca tcatcccggc cggtctcgaa atgatggaca ggaaggccat
      781 ccacgctgtc gagccctacg tcaacgccgg ttacgacatg gacgccgaag cgatcctgct
      841 gtgcgaatcg gatggcacgc cggaagaagt cgccgaggag atcgggcgca tgacggcagt
      901 gctggaaagg agcggcgcct cggcctattg cgtttcgcgc accgaagccg agcgcctcaa
      961 gttctgggcc ggacgcaagg cggcgttccc ggctgttggt cggttgaccc cggattatct
     1021 gtgcatggac ggcaccattc cgagaaagcg gttggccgaa atgctccagg aaatcaccgt
     1081 gctttccggg aaatacgatc tgcgttgcgc caacgtcttc cacgccggcg acggcaatct
     1141 gcatccgctg atcatgtacg atgccaatca gcccggacag cttgagcggg cgatggcttt
     1201 cggtgccgaa atcctggaac tgtcggtacg cttcggaggc accatcaccg gcgagcatgg
     1261 ggtggggatc gagaaggtca atctgatggc cgagcagttc ggaatggccg agatcgaaac
     1321 ctttcatcgc ctcaaggcgg ccttcgacga gcatgggctg ctcaatcccg gcaagggcgt
     1381 gccgacgctt gcccgttgcc gcgaatatat ccaggcgcgc gtggcgcgtg cgccgactgc
     1441 gccgaggttc tgatggaaac actgatcgac gactggcgcg cacgcattgc cgctgcggcc
     1501 ggttcgggcg gcaagctggc gattcgtggc ggcggcagca agtgtttcta cggtggtccg
     1561 gagaccggcg agcggctcga tgtcgaggcc taccgcggga ttgtcgccta cgagccaagc
     1621 gaactggtgg ttaccgcacg tgccggaacg ggtctgcgcg aactggcggc aacgctcgcc
     1681 gcgaaggggc agggcctggt cttcgagccg ccgcatttcg gggccgcggc gacgctcggc
     1741 ggcatgctcg ccaccggttt gtcgggacca cgccggcagg cggtgggtgc ggtgcgcgat
     1801 ttcgtgctcg gcgtcaaatt gctcaatggc agcggcgaag tgctctcctt cggcgggcag
     1861 gtgatgaaga acgtcgccgg ctacgatgtc ccgcgcttgc tggcgggcag cctcggcacg
     1921 ctcggcgttg tgctcgaagt ttcgctcaag gtgctgccgc tgccggtggc cgagaaaagc
     1981 ttgcgttttg cgctcgacga ggccgcggcg ttgcagaagc tcaaccagtg gggtggccgg
     2041 ccattgccga tttcggcgtc ggcgtggcac gacggcctgc tgacgctgcg cctgtccggg
     2101 gctgccgccg cggtcgctgc cgcagagcgc gaactcgctg gcgaggtgct ggccgatgcc
     2161 gacgccttct gggattcgct gcgcgagcag aagcaggcgt tctttctcgg cgacgcgccg
     2221 ctgtggcggc tttcgctgcc gtccgtgacg ccgccgcagg cgctcgggga gacgctgatc
     2281 gaatggggcg gcgcgcagcg ctggctgcgc ggtggcgacg ccaggcggat tcgcgcggcc
     2341 gcggcaaagg ccggcgggca tgcgaccctg tttcgaggtg acgatgccct gaagacggag
     2401 gtcggcgtct tccagccgct gaccgcgcct ctggcgcgca tccatcgcca tctgaagcag
     2461 gccttcgatc cgcagggggt tttcaacccc ggccggatgt atccgggtct atgaaagttc
     2521 gccgatcatg caaaccaatc tagccgattt catcaaggac acgccagtcg ggcgcgaggc
     2581 cgacgcgatt ctgcgcagtt gcgtgcattg cggcttctgc aacgcgactt gtccgacctt
     2641 tcagttgctc ggcgatgaac tcgacgggcc gcgcggtcgc atctatctga tgaaacagat
     2701 gctcgaaggg catgcggtca ccacccggac gcaactgcat ctcgatcgct gcctgaattg
     2761 ccgcgcctgc gaaacgacct gtccttcagg cgtcaggtat catcgcctgc tggagatcgg
     2821 acgcgcggtc atcgagcaac gtgtaccgcg ttcacccgcc gcccgggcga cgcgtttcct
     2881 gctttgcgaa gcgctgccgc gcaactggat tttcaagccg gccgtgcgtg tcggacaact
     2941 gttgcggccg ctgttgccgg ccgccctggc cgacaaggtg ccgagcgctg ccggtggggt
     3001 gcgcagaccg tggccgcggc cggccgcgcg gccacgcagg atgatcgccc tggccggttg
     3061 cgtgcagccg accctgaccc cgaataccaa tgcggcgatg gcacgtgttc tcgaccggct
     3121 cggtatcgaa ttgtgcgaag tgccgggcgc cggctgttgt ggcgcgctgc gctaccacct
     3181 caatgctcag gaaaaggcac tcgacgacat gcggcgggtg atcgacgcct ggtggccgca
     3241 tgtcgagaac ggttccgtcg agggcttcgt gatgaccgcg tcgggctgcg gtgcgcacgt
     3301 gcgcgaatac ggtgaactgc tcaggcacga tccggcgtac gccgacaagg cggcgcgaat
     3361 tgcggccatg acgcgcgatg cctccgaggt cctggctgcc gagcagcaac aactcgtcga
     3421 gttgctgcgt cggcagggcg atggcagcgg ccagcgggtg gcgtttcact cgccctgcac
     3481 cttgcagcac gcgcagaaga ttcgcggtgt cgtcgaggcg ctgctgactg ccgccggtta
     3541 ccagctctcg ccggttgccg attcgcacct gtgctgcggc tcggcgggga cctattcgat
     3601 cagtcaaccg atactcgcca ggcagttgcg tgacaacaag ctggcggcga tgcgcgcggg
     3661 ttcgccggcg ctgctggtga cggccaatat cgggtgccag acccacctgc aaagcggcag
     3721 caagataccg gtacggcact ggatcgaact gatcgacgag cgcctgggcg cagcctcctg
     3781 agcagccgat gcggagacag gatggaactc gacagtcagg cgggtagtta ccaggagctt
     3841 tgccggagct ttcgctggca gatcccggag cagttcaacc tcgcagtgga catctgcggc
     3901 cgctgggccg agcaacgtca ccgcttcgcg ctgtactacg aggacgagag cggtttcacc
     3961 tcggcacaca ccttctggga tattcagcga caggcgaacc ggctctcgaa cgtgctcgcg
     4021 gcattgggta cgctgcctgg cgaccgggtg gcgatcctct tgccgcaatg cccggaagcg
     4081 gccatcgcgc agatcgcgat ttgccagatg ggcgcgctgg ccgtccctct gtcgccgttc
     4141 ctggggccgg attcactgga acaccgtttg ggcgatgccg ccgcccacct ggccatcgtc
     4201 gatgcgacga cgcagcccgg agtgctaggg ctgcgccaga ggttgccgcg gttgcgacac
     4261 gtgctcggcg ttggcagcgc cgcaggacca ggcatccggc cctgggatga agtgctcgag
     4321 cacgcttcgg cgcgctacac gccgacagtc accgcggccg gcgatccggc gatgatcctt
     4381 taccgccgcg gcacccaggg cggcaccagg ggtgccttga tggcgcaccg aatgctgttc
     4441 ggcaagctca gtgcgttcgt ctgcgcgcat gacttctttc cgcaaccgcg ggacatgctc
     4501 tggtcgcctg gcgaacccgc gtgggccggc ggcttttggg atgccctgct gccgacctgg
     4561 catttcggca tgccgctgct cgcgtacaac gggcccctgg acgccgtgaa ggtctttgcc
     4621 ctgatcgaaa agtacggtat tcgcaatgcc gttctatggc cgacgctctt gcagcggatg
     4681 atgcagcggg tggcggaccc cggcgcggtc tacgatctcg acctgcgtac gctggcgagc
     4741 gtcggcgagc cggtcgatcc agcgctgtgg cactgggcga gagacaaaat gggtgtggcc
     4801 atcaacggtg tcttcgggcc tgccggcctc cccgatctgg ttggcaactg ggcttcgcga
     4861 tggcctgcca gtccgggcgc ggtgggcaga gcctatccgg ggcaccgggt ggcggtgatc
     4921 gatgctcagg gaaacgcgct gccgccgggc gaggtcggcg agctcgccgt gtttcgacag
     4981 taccatgctg aggatgatcc cctgccgatg ctcggctatt ggcgggatcc cgaggccacg
     5041 tcagaggccg tcgtcggcga tggctgggtg cgtaccggcg aatcggcgac gctcgatgga
     5101 gagggtaatc tattctgcca gcggcccgcc gacgatacct tcgcgagtgc tggctatcgc
     5161 gtcgacccgt cggagatcga gacctgtctg cgtgcgcatg ccgacgttgc caactgtgcc
     5221 gtgatcggcc gtgcggacga gctgggcggc acacggccca gggctttcgt cgtcctgcgg
     5281 ccgggcgtgc tcgggacgcc ggaactggtt cgcgagttgc agcgacatgt cggccggtat
     5341 ttggcgccct acgaaacgcc gaaagcgata gaattcaccg cggcgctgcc gctcggcgcc
     5401 agcggggagg tcttgcggtg cgccttgagc acacgcgaag cgtaaccgac ggcatgcaca
     5461 gtaggtggaa tgagggggag gcaatgagcg aacagcttga gcaggcagcc ggcctggtgg
     5521 acgagcgacg tcagaaccag cgctcgcggg ccgctttcga cagcgctttc gtgatgatcg
     5581 agccgttttt cgatccaggg cagggctggg gggggcagtc gctggaactt ctggcgtacc
     5641 gtatcgtgcg cgagaatttt cctgaactga gtggcgagga ggtgcatgcc gtggtggtgg
     5701 cagcacaccg cgtgtatatc gagcgcaatc cgcaacgtag cgatcacctg ccgcgcccga
     5761 gcgaactacg gcgggtaacc ctctgaaccg gcatgcggtg ccttgggggg aggcggtaac
     5821 gcctgccgtg gcgcgcctga agtggctgcc gagtttgcgg agaaagcctg tcgatgccga
     5881 tttcgattga tgcctgtgtt gcccagcatc agggcgaccg tcgcgagcaa caggaccggg
     5941 tcgccatcct gccgcatccg cggggcggcg gggtggcgtt ggcggtgttg gcggacggca
     6001 tgggcggcca taccggtggg gtcattgccg cccagcaggt gatccacacc gcgcgcaaca
     6061 acctcgaggg atattcggcg cgaaccgaat cggcgcgcgt gctgctggaa tcgtgcatca
     6121 acgaagcgca tatgctgatc aaggcttccc gtttcatcaa cgagaaggat ccgcacagca
     6181 ccgtggtgat gttgctgctg cagccgggcc gggtgagctg ggcacactgc ggtgacagcc
     6241 gtttctacca cttccgcggc gatgccttgc tcttccgcag caccgatcac tcgtacgtgg
     6301 cgcaattggt ggtgcaaggc cggatcactc ccgaacaggc gctggtccat cccaaccgca
     6361 acattctgct gacctcgctg ggcggcggcg acgctcccaa gattgccttc ggcgaagccg
     6421 aaggcctgca ggcaggggac accttcctgc tctgctccga cggtctctgg ggctatttca
     6481 ccgaccagga actggcctgg gtgatcagcg gctcgtcttc ggcgcgggaa gcgtccgagt
     6541 tgctgatcgg gcgcgcgcgc tcgatggggc atggcgacgg cgacaacatc tcggtggcca
     6601 tcctgaagct ggtcaatgtg ccccagccgg agccggcggc cacaccaggc gcaaggacga
     6661 cgctacttgc gcccgaggcc gccaagtagc gcggcgacca ggcggcgtgg tttgtgcggg
     6721 tgctcactgc gggccagggc cctttcggcc tcgctgccga ctgctgccgc cactggatgc
     6781 ggttccgggc tttcgtaagg cttgctgaag tcgaaaccgt cggcggcgat gtgcgacggg
     6841 cgtcggccac tgcccggccg gttcgatcct tcacgcgccg ccgcgcggct cggggcgcgc
     6901 tcgcccggtg ctgtcgccag tggcgccagg gctggcggag ctgccgttgg ccggcggtgg
     6961 tgactgcgtt tgtcgccggg cgggtagcag tagtcgtatt ccggctcgaa gccggggatg
     7021 gctacctgct c
//
LOCUS       DAOSIT010000040         7010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9772_length_7010_cov_9.328541, whole genome shotgun sequence.
ACCESSION   DAOSIT010000040 DAOSIT010000000
VERSION     DAOSIT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9772_length_7010_cov_9.328541"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLL14_01905"
     CDS             <1..267
                     /locus_tag="PLL14_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOG02701.1"
                     /translation="ETVTGEHTGWAAWSPDQAAMARDRYEFHRVRKARDATAHAEKLE
                     KKAEMKLADLAAHSQHTDPLILDKKRLVIEAALARARAKRAGGD"
     gene            complement(306..719)
                     /locus_tag="PLL14_01910"
     CDS             complement(306..719)
                     /locus_tag="PLL14_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG02702.1"
                     /translation="MSQRHVISLGPQATIWDAACLMTKANCGSVLIVDPASALLGIVT
                     ERDLMTRVLAKALNPATTLVSEIMTRNPRCVGPDVLVSDAVLIMIERGFRHLPIMGEG
                     SRILGVFSVRDALPREIGTAVEMAEFHEQVNDALG"
     gene            complement(803..1858)
                     /locus_tag="PLL14_01915"
     CDS             complement(803..1858)
                     /locus_tag="PLL14_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOG02703.1"
                     /translation="MTYIAKPRLHHPTLTTNKVGYTRRDYEGRISTLCAGCGHDSISA
                     AIIQACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANLANR
                     DLLYLGVSGDGDSASIGLGQFANAMRRGVRMAYIVENNGVYGLTKGQFSATADRGSKS
                     KKGVVNSDSPVDLVGIALQLGATFVARSFSGDKAQLVPLIKGAMAHGGAAFIDVISPC
                     VTFNNHGGSTKSYDYVRQHNEAVSRIDFMTPRSEITTEYAPGEVVDVPQHDGTVLRLR
                     KLHEDYDPTDRYAAMSYMNEHQSRGEVVTGLLYLDPLATDLHTAMNTSHRPLNTLGRA
                     ELCPGVGALEKINASLR"
     gene            complement(1855..>2853)
                     /locus_tag="PLL14_01920"
     CDS             complement(1855..>2853)
                     /locus_tag="PLL14_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG02704.1"
                     /translation="ARAFTATSGPGVSLMTEFIGLAYFAEIPVTIINVQRGGPSTGMP
                     TRTQQSDLLSCAYASHGDTKHVLLFPQDPHECFEHSAAALDLADRLQTPIFVMTDLDI
                     GMNQRLCKPFSWDGARDYDRGKVMTAEELEAGKDFGRYKDVDGDGIPWRTLPGTHPTK
                     GSFFTRGTTRNPYAQYSERGPDYIYNMERLLRKFRTAADLVPQPVLREATQPTPYGVI
                     YFGSTTPAMREALDVLEADGQHLDALRLRAFPFPDSVSEFIATHEKVFVVEQNRDAQM
                     RSMLVNELHFDPARLVPVLHYDGTPITARFIVQAIRDNIQPASANASAPARQKETA"
     assembly_gap    2854..2953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2954..3312)
                     /locus_tag="PLL14_01925"
     CDS             complement(<2954..3312)
                     /locus_tag="PLL14_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG02705.1"
                     /translation="MNRIEAINDFVIKFANVNGSGSASANELFSKAVMRMGVPVSPRN
                     IFPSNIQGMPTWYEARVCEKGYHGRRGGVDMMVAMNPQTWDTDIAEIEPGGYLFYDST
                     RPIPESRFRKDVHVIGMP"
     gene            complement(3309..5135)
                     /locus_tag="PLL14_01930"
     CDS             complement(3309..5135)
                     /locus_tag="PLL14_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG02706.1"
                     /translation="MQPTNTTRPDYFHKVVDCQWACPAHTPVPEYIRLIGQGRYSDAY
                     MINWVSNVFPGVLGRTCDRPCEPACRRGRVEENNGAAPEPVAICRLKRVAADNKGEVK
                     ARMPAIAAPNGKRIACVGAGPASLTVARDLAPLGYEVTVFDGEAKAGGFIRSQIPRFR
                     LPESVIDEETGYILDMGVKFRSGERVESMQALLKQGYDAVFVGCGAPRGRDLDVPGRS
                     EAAAHIHIGIDWLASVSFGHVTNVPPRVIVLGGGNTAMDCCRSARRMGGSDVKVIVRS
                     GFEEMKASPWEKEDAIHEGIPIINFHVPKSFQHKDGKLLGMTFEIVRAVYDEKGRRTL
                     VPTGQPDAFFECDAVLVAVGQENAFPWIESDCGIQFDKWGLPVLGQETFQSTVPQVFF
                     GGDAAFGPKNIITAVAHGHEAAVSIDRFLNGESVATRPAPRVNLMSQKMGIHEWSYHN
                     DVSGDTRFKVPWAKAETALASIRVEVELGFDAATAFKEASRCLNCDVQTVFNRDTCIE
                     CDACVDICPMDCITFTGNGDEADLRTRLKAPAMHTAQDLYVSAELKTGRVMVKDEDVC
                     LHCGLCAERCPTGAWDMQKFLLNTTQAGPGCRDARKAHLEVA"
     gene            complement(5313..5795)
                     /locus_tag="PLL14_01935"
     CDS             complement(5313..5795)
                     /locus_tag="PLL14_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG02707.1"
                     /translation="MQRPFKVLGIQQIAIGGPDKKRLQTLWVDMFGLEVTGTFRSERE
                     NVDEDICAIGSGPFKVEVDLMQPLDPDKKPAVHTTPLNHIGLWIDDLPLAVQWLSAQG
                     VRFAPGGIRKGAAGFDITFLHPKGNDEFPIGGEGVLIELVQAPPEVVSAFARLAAAAD
                     "
     gene            complement(5826..>7010)
                     /locus_tag="PLL14_01940"
     CDS             complement(5826..>7010)
                     /locus_tag="PLL14_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HOG02708.1"
                     /translation="TRLQVEHPVTECITGLDLVELMIRVAAGEKLPITQADVKREGWA
                     IECRINAEDPFRNFLPSTGRLVRFAPPAQTMFQSDTGQRYGVRVDTGVQDGGEIPMYY
                     DSMIAKLIVHGTDRNDAIAKMREALNGFVIRGISSNIPFQAALLAHPRFVSGEFNTGF
                     IAEHYAKGFRAEDVPHDDVNFLVAMAAFVRRKSRERAAGMSGQLPGYGVKVGVDYTVV
                     VLSENGHAEYRYVPVHVDEFTGKAGAAAIHVEGKLYEVNSGSRLNDICIEGTVNGEPF
                     TAQMERGTAKNPLALRVQHNGTKIEALVMSPRMAELHRLMPFKAPPDMSRYVLSPMPG
                     LLVDVAVQPGQKVQAGERVAVIEAMKMENVLFAAADGVVGKVLAAKGESLTVDQPIIE
                     FV"
BASE COUNT         1256 a         2156 c         2179 g         1319 t
ORIGIN      
        1 gaaacggtca ccggtgagca caccggatgg gcggcctggt cgccggatca agcggccatg
       61 gcccgggacc ggtacgaatt ccatcgcgtt cgcaaggcgc gcgatgcaac cgcccatgcg
      121 gaaaagctgg aaaaaaaggc cgaaatgaaa ctcgccgacc ttgccgcaca ttcccaacac
      181 accgacccgc tgatcctgga caagaagcgt ctggtgatcg aggcagcttt ggcgcgtgcc
      241 cgcgcgaaac gcgccggagg cgactgaact ggcccctgct ggaccagcag gtcagcaagc
      301 tcggatcatc ccaatgcgtc gttgacctgc tcgtggaact cggccatttc gaccgctgtt
      361 ccgatctccc tgggcaaggc gtcgcgcacc gagaacacgc cgagtatgcg agacccctca
      421 cccatgatgg gcaggtgacg gaaccctcgc tcaatcatga tcagcaccgc gtcggaaacg
      481 agcacatcgg gcccaacgca gcgcgggttg cgcgtcatga tttcagagac cagggttgtc
      541 gcaggattga gcgccttggc cagcacccgg gtcatgagat cccgttcggt aacgatgccg
      601 agcagagcgc tggccgggtc cacaatcagc acgctgccac agttggcctt ggtcatcagg
      661 cacgcggcgt cccagatcgt tgcctgtggc ccgaggctga tgacatgtcg ttgggacatg
      721 gattgaaaaa ccgtgcgttc aggcatgatg gcagcccttt caaatgacat tgtggaatcg
      781 gatttcctgc agcggcatgg gcctagcgca gggacgcgtt gatcttctcc agcgcgccaa
      841 cgcccgggca gagttcggcg cgtccgaggg tgttgagggg ccggtggctg gtgttcatgg
      901 ccgtgtgcaa atccgtcgcc agcggatcca ggtaaagcag gccggtcacg acctctccac
      961 gtgactggtg ctcgttcatg taactcatgg cggcgtagcg gtcggtggga tcgtaatcct
     1021 cgtgcagctt gcgcaggcga agcacggtgc catcgtgctg gggcacatca accacttcac
     1081 ccggcgcgta ctcggtggtg atttcgctgc gcggggtcat gaaatcgatg cggctgacgg
     1141 cctcgttgtg ctgacgcaca tagtcgtagc tcttggtgct tccgccgtgg ttgttgaagg
     1201 tgacgcaggg gctgatcaca tcgatgaacg cggcaccgcc atgggccatg gcacctttga
     1261 tcagtggcac caactgggct ttgtcgccgg agaaactgcg cgcgacgaaa gtggcgccca
     1321 gttgcagggc gatacccacc aggtcgacag ggctgtcgct gttcacgacg cccttcttgc
     1381 ttttcgatcc acggtccgcc gttgcagaga actgcccttt ggtcagaccg tagacgccgt
     1441 tgttctcgac gatataggcc atacggacgc cacggcgcat ggcgttggca aactgaccca
     1501 gtccgataga ggcggaatct ccgtcgcccg acacgccaag gtacagaaga tcccggttgg
     1561 ccaggttggc gcccgtcagc accgatggca tgcggccgtg caccgtattg aaaccatggg
     1621 aggcccctag gaagtagtcg ggcgtctttg agctgcaacc gatgccggaa agcttggcga
     1681 cacggtgggg ttcgatatcg agttcccagc aggcctgaat gatggccgcg gaaatcgagt
     1741 cgtgcccgca tcctgcgcac aaggtcgaga tgcggccctc gtagtcacgc cgggtgtagc
     1801 ccaccttgtt ggtcgtgagc gtcgggtgat gcagtctggg tttggcaatg taggtcatgc
     1861 ggtctccttc tggcgggccg gggctgaagc gtttgccgac gcgggttgaa tgttgtcgcg
     1921 tatggcctgg acgatgaacc gtgccgtgat cggagtaccg tcatagtgca ggaccggaac
     1981 caggcgcgca ggatcaaaat gaagctcgtt gaccagcatc gagcgcatct gcgcatcacg
     2041 gttttgctcc accacgaaaa ccttctcgtg cgtcgcaatg aactcgctga ccgagtcggg
     2101 gaacgggaag gcgcgaagac gcagggcgtc cagatgctgg ccgtcggctt ccagcacgtc
     2161 cagcgcttca cgcatggccg gcgtggtgga cccgaaataa atgacgccat aaggggtcgg
     2221 ttgggtcgcc tcacgaagca caggttgcgg aacaaggtca gcggccgtgc ggaatttgcg
     2281 cagcaggcgc tccatgttgt agatgtagtc ggggcctcgc tcggaatact gcgcatacgg
     2341 gttgcgggtg gtgccccggg taaagaaact ccccttggtc gggtgggtcc ccggcaacgt
     2401 gcgccaggga ataccatctc catcgacatc cttgtagcgg ccgaagtcct tgccggcctc
     2461 gagttcctcg gccgtcatga ctttgccccg gtcatagtcg cgggccccgt cccagctgaa
     2521 tggcttgcac aggcgctggt tcatgccgat gtcgaggtcg gtcatgacga agatcggtgt
     2581 ctgtagccgg tctgccagat cgagtgcagc agccgagtgc tcgaagcatt cgtgcgggtc
     2641 ctggggaaac agcaacacat gtttggtgtc accgtgcgat gcataggcac aggaaagcag
     2701 gtcggactgc tgcgttcggg tcggcatgcc cgtggacgga ccgccccgct gcacgttgat
     2761 gatggtgacg gggatttccg caaagtaggc caggccgatg aactcggtca tcagcgagac
     2821 gcccggccca gatgtggccg tgaacgcacg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnggcatcc cgatcacgtg aacgtccttg cgaaaacgcg attcagggat
     3001 cggccgggtg ctgtcgtaga acaggtagcc gcccggctca atctcggcaa tgtcggtgtc
     3061 ccatgtctgg ggattcatgg cgaccatcat gtcgaccccg ccgcgccgtc cgtgatagcc
     3121 tttttcgcac acccgcgcct cgtaccaggt cggcatgccc tggatgttgc tcgggaagat
     3181 gttgcggggg ctcaccggca cgcccatgcg catcacggcc ttggaaaaga gttcgttggc
     3241 cgaagccgat ccggagccgt tgacgttggc gaacttgatg acgaaatcgt tgatggcttc
     3301 aatgcggttc atgcgacctc cagatgtgct ttgcgtgcgt cccgacagcc aggtccggcc
     3361 tgcgtcgtgt tgagcaggaa cttctgcatg tcccatgccc cggtcgggca gcgttcggcg
     3421 caaaggccgc agtgcaggca gacgtcctcg tccttgacca tgacccgccc ggtcttgagt
     3481 tcggccgaca cgtacagatc ctgtgccgtg tgcatcgcgg gtgctttcag gcgggtgcga
     3541 aggtccgcct cgtctccgtt gccggtgaag gtgatgcaat ccatcggaca gatgtccaca
     3601 caggcatcgc attcgatgca ggtatcccgg ttgaaaacgg tctggacgtc gcaattcagg
     3661 caccggctgg cttccttgaa cgcagtggct gcatcgaacc ccagctccac ctccacgcgg
     3721 atgctggcca gcgccgtctc ggccttggcc catggcacct tgaagcgggt atctcccgac
     3781 acgtcgttgt ggtagctcca ttcatggatg cccatcttct gcgacatcag gttgacccgc
     3841 ggcgccggtc gggtggccac cgactcgcca ttcaggaagc ggtcgatgga aaccgctgct
     3901 tcatggccgt gagcgacggc cgtgatgatg ttcttgggcc cgaaggcggc atcgccacca
     3961 aaaaagacct gcggcacggt ggactggaag gtctcctgcc cgaggactgg aagcccccac
     4021 ttgtcgaact ggatcccgca gtccgattcg atccagggga aggcgttttc ctgcccgacg
     4081 gccaccagca cggcgtcgca ctcgaaaaat gcgtctggct gccctgtggg aacgagcgtg
     4141 cgccggcctt tttcgtcgta gaccgcccgc acgatttcga aggtcatgcc caacagcttg
     4201 ccatccttgt gctgaaagct cttcggcaca tggaaattga tgatcggaat gccctcgtga
     4261 atggcgtcct ccttctccca cggcgaggcc ttcatctcct cgaaaccgct gcgaacgatg
     4321 actttcacgt cggagccccc catgcgacgg gcagaacggc agcaatccat cgcggtattg
     4381 ccaccaccga gcacgatgac acgtggcggc acattggtga catggccgaa tgaaaccgag
     4441 gccagccaat cgatgccgat gtggatgtgt gcggccgcct cgctgcgtcc gggcacgtcg
     4501 agatcgcgtc ctcgcggtgc cccgcatccg acgaacacgg cgtcgtaccc ctgcttgagc
     4561 aaggcctgca tggactccac gcgctcgccg ctgcggaact tcacgcccat gtcgaggatg
     4621 tatcccgttt cctcgtcgat caccgattcg ggcagacgga agcgtggaat ctgcgagcgg
     4681 atgaagccgc ccgccttggc ttcgccgtcg aacacggtga cctcgtatcc gagcggggcc
     4741 agatcgcgtg ccacggtcag ggaggcggga ccggcgccga cacaggcgat gcgctttccg
     4801 ttcggagcag caatggcggg catgcgcgcc ttgacctcgc ccttgttatc tgcagcgacc
     4861 cgcttcagcc ggcaaatcgc aaccggctcc ggtgccgctc cgttgttttc ctcgacgcgg
     4921 ccacgtcggc aggcgggctc gcatgggcgg tcgcaggttc gacccaggac acccggaaag
     4981 acattcgaca cccaattgat catgtaggca tcgctgtagc ggccctgacc aatcaggcga
     5041 atgtattcgg gtacgggggt gtgggccgga caggcccact gacaatcgac gaccttgtga
     5101 aaatagtcgg gccgggtggt gtttgttggc tgcaagacgg tctccttcac cagcaatggc
     5161 gccaggggcg gcgccacggg tgcgggcagt ctattccttc aggcacccat tcggtgcgac
     5221 cggtcgggca aaaagcgccg atctatgacc caaatcaaga attttgtgcc ccaaccccga
     5281 gcacgaccga cgcggatcgc gctcagagtc tctcagtcgg cggcagcggc caggcgcgcg
     5341 aatgccgata ccacttccgg cggcgcctgc accagttcaa tgagcacgcc ttcgccgccg
     5401 atcgggaatt cgtcgttgcc cttcggatgc aggaaagtga tgtcgaaacc ggcagccccc
     5461 ttgcggatgc cgcccggcgc gaatcggacg ccttgcgccg acagccattg caccgccaac
     5521 ggcaggtcgt cgatccacag gccgatgtgg ttcagtggcg tcgtgtgcac cgccggtttc
     5581 ttgtcggggt ccaacggctg catcaggtcc acctcgacct tgaagggccc gctgccgatg
     5641 gcacagatgt cttcatcgac gttctcgcgc tcgctgcgga aggttcccgt cacttccagg
     5701 ccgaacatgt cgacccaaag cgtctgcagg cgtttcttgt cgggcccgcc gatggcgatc
     5761 tgctggatgc ccagcacctt gaatggtcgt tgcatgagtg attgatccag ggttgtcact
     5821 gccgctcaga cgaactcgat gatcggctgg tccacggtaa ggctctcgcc cttggccgcc
     5881 agaaccttgc ccaccacccc atccgctgcc gcgaacagca cgttctccat cttcatggcc
     5941 tcgatcacgg cgacccgctc gcctgcctga accttctggc cgggttgaac ggccacgtcg
     6001 accagcaggc cgggcatcgg cgacagcacg tagcggctca tgtccggcgg cgccttgaag
     6061 ggcatcagcc gatgcagctc cgccatgcgt ggcgacatca ccagggcctc gatcttggtt
     6121 ccgttgtgct gcacgcgcag ggccagcggg ttcttggccg tgccgcgctc catctgcgcc
     6181 gtgaaaggct cgccattgac ggtcccctcg atgcagatat cgttcaggcg cgaaccgctg
     6241 ttgacttcgt acagcttgcc ctccacatgg atggcggccg caccggcctt gccggtgaat
     6301 tcgtcgacgt gcaccggcac ataccggtac tcggcatgtc cattctccga cagcaccacg
     6361 acggtgtagt cgacacccac cttcacgccg tagccgggga gttgcccgga catgcccgcg
     6421 gcgcgttcgc gcgacttgcg gcgcacaaac gcggccatgg caacgaggaa attgacgtcg
     6481 tcgtgcggca catcctccgc ccggaagccc ttggcgtagt gttcggcgat gaagcccgtg
     6541 ttgaattcac cactgacaaa ccttgggtgc gccagcagcg ctgcctgaaa gggaatgttg
     6601 ctgctgatgc cgcggatcac gaatccgttg agcgcctcgc gcatcttggc gatggcgtcg
     6661 ttgcggtcgg ttccgtgcac gatcagcttg gcaatcatcg agtcgtagta catcgggatc
     6721 tcgccgccat cctgcacgcc ggtatccacg cgcacaccgt atcgctggcc ggtgtcggac
     6781 tggaacatgg tctgcgctgg cggcgcgaat cgcaccagac gcccggtaga cggcaggaag
     6841 ttgcggaacg gatcctcggc gttgatgcgg cactcgatgg cccagccctc acgcttgaca
     6901 tcggcctgcg tgatcggcag cttttcgccc gcggccacac ggatcatcag ctccacgaga
     6961 tccaggccag tgatgcactc ggtcaccggg tgttccacct gcagccgggt
//
LOCUS       DAOSIT010000041         6994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9796_length_6994_cov_10.033434, whole genome shotgun sequence.
ACCESSION   DAOSIT010000041 DAOSIT010000000
VERSION     DAOSIT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9796_length_6994_cov_10.033434"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1426
                     /locus_tag="PLL14_01945"
     CDS             <1..>1426
                     /locus_tag="PLL14_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG02709.1"
                     /translation="MKQAILAAEDERFYQHGGVDTQGVLRALYANLVSGGKRQGASTI
                     TQQVAKNFFLSSEKTYTRKLYEALLSFKIEHNLSKDQILELYINQIFLGQRAYGFAAA
                     AQIYFGKPLGELTLAEAAMLAGLPKAPSTFNPVINPKRARLRQQYVLRRMNELGFITD
                     AQQEAALQQPLVIKREVNSTPVHAGFVTEMARQIAAERFPDDVYTRGLRVYTTIVRED
                     QEAAYNSVRRGVMDYDRRHGYRGAEAYVDISEIKSEQDEALDELLLEFQDAEDLHPAI
                     VLNVDSKRIRAYRRGGEIVTVSGEGLKFAARMIDDKAPPNKRLRRGAVIRVQTDDKDN
                     WRIVQLPEVEAAFLAADPHDGAIRALVGGFDFNRNQFNHVTQAWRQPGSSFKPFIYSG
                     ALEKGFTPASVINDEPISFPSSVTGSQSWEPKNYDGKYEGPMRMRTALAKSKNMVSIR
                     LLQASGVRFVQDYVTRFGFEASKHP"
     assembly_gap    1427..1526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1527..1871
                     /locus_tag="PLL14_01950"
     CDS             <1527..1871
                     /locus_tag="PLL14_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOG02710.1"
                     /translation="LAGKTGTTNEHVDAWFCGYQQTVVACSWIGFDQPRNLGSGETGG
                     TAALPTWISYMAKVLKDVPESFLPAPEGVVAVASPSSSGKGPAEELFYQENAPAELEP
                     EPPVDHSIKPED"
     gene            2105..2305
                     /locus_tag="PLL14_01955"
     CDS             2105..2305
                     /locus_tag="PLL14_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02711.1"
                     /translation="MKVMMVVEGGDCALRIEPEDQEGRALLAAFGVTGEFRTRLGSSA
                     PAPAVSAAQVAAIFEGAPAELD"
     gene            2482..>4034
                     /gene="msbA"
                     /locus_tag="PLL14_01960"
     CDS             2482..>4034
                     /gene="msbA"
                     /locus_tag="PLL14_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOG02712.1"
                     /translation="MSSASQPPPSSLRIYFRLLGYLRPLLGLFGVSLLGYLMFASSQP
                     MLAGILKYFIDGLNTAGGKIPYALPLLGEMDLIHALPVLLVVVVSWQGLGSFLGNYYL
                     ARVSLGLIDDLRRGLFDSLLRLPNHYFDQQNSGHLISRITYDVTMVTGAATEAIKVVI
                     REGMTIVFLFGYLLWMNWRLTLVMVAILPIIALMVASASRKFRKQSRKIQVAMGDLTH
                     VASETIQGYRVVRSFGGEDYESARFRAASADNTAKQLRMVRTAASYTPALQFVTFSTM
                     AALLVLVLLLRGDASAGDLVAYITAAGMLPKPIRQISEVSGTIQKGLAGAESIFAQLD
                     EAPEPDLGTVERERVEGRLQVSDLSFAYPGSTQRVLDKVSFSVEPGQMVALVGRSGSG
                     KSTLASLIARFYNHDSGHILIDGVDVEDYTLRNLRRHIALVTQQVVLFNDTVAGNIAY
                     GDLASAPRAAIEAAAEAAFAREFIDRLDAGFDTLIGENGVMLSGGQRQRLAIARALLK
                     DAPILILDEA"
     assembly_gap    4035..4134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4135..5438)
                     /locus_tag="PLL14_01965"
     CDS             complement(<4135..5438)
                     /locus_tag="PLL14_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOG02713.1"
                     /translation="MAATKPHAPGPGARPGDEFPALVDAATLRAAGRDPGLPFRIALA
                     DGRQLGMRRLLRVLPGKRLVGEAEIDGCRVLAKLFVGRRSAKHWRQERTGLAALAAAA
                     VPTPALLAATPLAGGGHALLTAFLDPAETLAEAWARLPRQSPDDAAALAVLAPAVRLL
                     GRLHAAGLVQEDLHLGNFLHHAGEVLVIDGDALRRVGRGQPLPMARACDNLAVLLAQL
                     PSAWDERLSILQAPYGEAAASAFPDPQALRARIAHVRAWRLRDFLGKTVRDCTLFAVE
                     HTLTRFTAVLRRQADALAALLAAPDAALDSAVVLKDGGTCTVARIVVDERVLLLKRYN
                     LKSLGHALNRCWRPSRAWHSWREAHRLQFLGIPTPAPLALVEERIGPLRRRAWLVSEF
                     CSGPNLLSHLAADREPPAAEAEAIVSLFAAMHRQRISHGDLKA"
     gene            complement(5425..6186)
                     /locus_tag="PLL14_01970"
     CDS             complement(5425..6186)
                     /locus_tag="PLL14_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOG02714.1"
                     /translation="MIDWWMNPQYASAAALFGDIDRVFALAGERITSDSLSRVLRVSA
                     AGKRYYVKCYSGTGKSPLRRWFGLRRWLGPPRVRAEWQNLLAFRAWGIPTATLVAYGL
                     ERRCGGFSRGALVTEEIPDTLDLAGMARAGDPRLRDRRWVEQVSRQVADITRTLHAAR
                     FAHNDLKWRNLLVSGGAQPTVYLIDCPCGGFYRGALLDYRMIKDLACLDKVGRKCLSR
                     SQRLRFYLDYTQHARATADDRRRIGKIVNFFNGRD"
     gene            complement(6183..>6994)
                     /gene="rfaP"
                     /locus_tag="PLL14_01975"
     CDS             complement(6183..>6994)
                     /gene="rfaP"
                     /locus_tag="PLL14_01975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HOG02715.1"
                     /translation="VFLDEPFRSLWAGRDPFVAVEALAGEVYRELEGRRTLRTEVAGR
                     GYFVKIHRGIGWAEIAKNLASLRLPVLGAANEWLAIQRLRELGVDSMRAVAFGLRGRN
                     PAQQLSFIITEELVPTISLEDYCLGWAEHPPSPRLKRALLVAVAQAARRMHLGGVNHR
                     DFYICHFLLHLQPPPTADRLRLSLIDLHRAQVRDITPRRWRDKDLAALYFSVLDMPFT
                     RHDRLRFLSAYFAQPLRRVLHDEAPLLARLASEAQRLQARFARKSAAGEMT"
BASE COUNT         1125 a         2248 c         2337 g         1084 t
ORIGIN      
        1 tcatgaagca ggcgatcctg gccgccgagg acgagcgctt ctatcagcac ggcggcgtcg
       61 atacgcaggg cgtcctgcgc gccctgtacg ccaacctggt gagcggcggc aagcggcagg
      121 gggcgtcgac gattacccag caggtggcga agaacttctt cctttcgtcg gaaaagacct
      181 atacccgcaa gctctacgaa gcgctgctgt cgttcaagat cgagcacaat ctgagcaagg
      241 accagatcct cgagctgtac atcaaccaga tctttctcgg acagcgcgcc tacggttttg
      301 ccgcggccgc gcagatctat ttcggcaaac cgctgggtga gctgacgctc gccgaagcgg
      361 cgatgctggc cggactgccc aaggcgccct cgaccttcaa tccggtgatc aacccgaagc
      421 gcgcgcggct gcgccagcag tacgtgctgc ggcggatgaa cgaactcggg ttcatcaccg
      481 atgcgcagca ggaagccgcc ctgcagcagc cgctggtgat caagcgcgag gtcaacagca
      541 cgccggtgca cgccggattc gtcaccgaaa tggcccgcca gattgccgcc gagcgttttc
      601 cagacgacgt ctatacacgc ggcctgcggg tgtatacgac gatcgtcagg gaagatcagg
      661 aggccgccta caacagtgtt cgccgcggcg tcatggatta cgaccgccgg cacggttatc
      721 gcggtgctga ggcctacgtc gacatcagcg agatcaagtc cgagcaggac gaggcgctcg
      781 acgagctgct gctcgagttc caggacgccg aggatctgca tccggcgatc gtcctcaatg
      841 tcgacagcaa gcggattcgc gcctatcgcc gtggtggcga gatcgtcacc gtcagcggcg
      901 agggcctgaa gttcgcggcc cggatgatcg atgacaaggc gccaccgaac aagcgcctgc
      961 ggcgcggcgc ggtgattcgc gtccagaccg acgacaagga caactggcgg atcgtgcaac
     1021 tgcccgaggt cgaagccgct ttcctggccg ccgatccgca cgacggtgcg atccgcgcgc
     1081 tggtcggcgg cttcgacttc aaccgcaacc agttcaatca cgtcacgcag gcgtggcggc
     1141 aaccggggtc gagcttcaag cccttcatct attccggcgc gctcgaaaag ggcttcaccc
     1201 cggcctcggt gatcaacgac gaaccgatca gcttcccctc ttcggtcacc ggcagccagt
     1261 cgtgggagcc gaagaactac gacggcaagt acgagggccc gatgcgcatg cgcaccgcgc
     1321 tcgccaagtc gaagaacatg gtctcgatcc gcctgctgca ggcgagcggt gtgcgctttg
     1381 tgcaggacta tgtcacccgt ttcggcttcg aggccagcaa gcacccnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnctgg cgggcaagac cggcaccacc aacgagcacg
     1561 tggacgcctg gttctgcggc tatcagcaga ccgtcgtcgc ctgttcgtgg atcggttttg
     1621 accagccgcg caatctcggc agcggtgaaa ccggcggcac cgccgcgctg ccgacctgga
     1681 tcagctacat ggccaaggtc ctcaaggacg ttcccgagtc gttcctgccg gctcccgagg
     1741 gcgtcgtcgc cgtcgcctct ccgagcagca gcggcaaggg cccggccgag gagctgttct
     1801 accaggaaaa cgcgcctgcc gagctcgaac cggagccgcc ggtcgaccac agcatcaagc
     1861 ccgaagactg acgactagcg ccggtagtac ggctgccggc gtacgctgcc gcggccggtg
     1921 tgaactagtt ccttccgctg tcggtgcgtg attggcgata cttccaccag aggtcacgat
     1981 tgcgccttgg ccctttgacc tttcagtcct tgacgtgagc agcgggcgcc agggcgactg
     2041 tcaagccgct gggtgagcag gcccgtctgt ccaccgtgag agccgggcga acatggaggt
     2101 cgaaatgaaa gtgatgatgg tggtcgaggg cggcgattgc gcgctgcgca tcgagcccga
     2161 ggatcaggag ggccgggcgc tgctggcggc tttcggagtc accggggaat tccggacccg
     2221 gctcgggagt tcggcgccgg caccggcggt atcggcggcg caggtcgcgg cgatcttcga
     2281 gggcgcgccg gccgaactcg actgaagccg caaccggtcg atgccgccgg ccatcgacac
     2341 gggtgaacct ggcaatacgc tcaggcgaga tgtcggcggc cggtgcggca gggtcggtct
     2401 gcggcgccgg tggagcgatc gggacggcag gttacagtgt tacactccgg cctcgcagtt
     2461 ccccgcaggc cgccgtctcc catgtcctcc gccagccagc ccccgccttc ctcgttgcgc
     2521 atctattttc gcttgctcgg ctacctgcgg cccttgctcg ggctgttcgg cgtcagcctg
     2581 ctcggctacc tgatgttcgc ctcgtcgcaa ccgatgctgg ccgggattct caagtacttc
     2641 atcgatggcc tgaacacggc cggcggcaag attccgtacg cactgcccct gctcggcgag
     2701 atggacctga tccacgccct gccggtgctg ctggtggtcg tcgtgagctg gcagggcctc
     2761 ggctcgttcc tcggcaacta ctatctggcg cgggtttcgc tcggcctgat cgacgacttg
     2821 cggcgcggtc ttttcgacag cctgctgcgg ctgccgaacc actacttcga ccagcagaac
     2881 tccgggcacc tgatctcgcg catcacctac gacgtgacga tggtcaccgg cgccgctacc
     2941 gaggcgatca aggtggtgat tcgcgaaggc atgaccatcg tcttcctgtt cggctatctg
     3001 ctgtggatga actggcgact gacgctggtg atggtcgcca tcctgccgat catcgccctg
     3061 atggtggcca gcgccagccg gaaattccgc aagcagagcc ggaagatcca ggtggccatg
     3121 ggcgacctga cgcatgtcgc gtcggaaacc atccagggtt accgcgtggt gcgcagtttc
     3181 ggcggcgagg actacgagtc ggcgcgcttt cgcgcggcca gcgccgacaa taccgccaag
     3241 cagttgcgca tggtcaggac cgccgccagc tacacgccgg cgctgcagtt cgtcaccttc
     3301 agcaccatgg ccgcgctgct ggtgctggtg ttgttgctgc gcggcgacgc ctcggccggc
     3361 gacctcgttg cctacatcac cgccgccggc atgttgccga agccgatccg gcagatttcc
     3421 gaagtcagcg gcaccatcca gaaggggctg gcgggcgccg agagcatctt cgcccaactc
     3481 gacgaggcgc ccgaacccga tctggggacc gtcgaaaggg agcgggtcga gggtcgactg
     3541 caagtcagcg atctctcgtt cgcctatccc ggcagtacac agcgcgtgct cgacaaggtc
     3601 agtttttcgg tcgaacccgg acagatggtg gccctcgtcg ggcgttcggg cagcggcaag
     3661 tccacgctgg cgagcctgat cgcccgcttc tacaaccacg attccggcca catcctgatc
     3721 gacggcgtcg atgtcgagga ctacacgctg cgcaacctgc gcaggcacat cgcgctggtg
     3781 acccagcagg tggtgctgtt caacgacacg gtagccggca acattgccta cggcgacctg
     3841 gcgtcggcgc cgcgggcggc gatcgaggcg gccgccgagg cggcctttgc ccgcgagttc
     3901 atcgaccgtc tggatgccgg cttcgacacg ctgatcggcg agaacggggt gatgttgtcc
     3961 ggcgggcagc ggcagcgcct ggcgattgcc cgggcgctgc tcaaggatgc gccgatcctg
     4021 atcctcgacg aggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccttc
     4141 aggtcgccat ggctgatccg ctgccggtgc atggccgcga acaggctgac gatcgcctcg
     4201 gcctcggcgg ccggcggttc gcgatcggcg gcgaggtggc tgagcaggtt cggcccgctg
     4261 cagaattcgc tgaccagcca ggcgcgccgg cgcaacggcc cgatgcgttc ctcgaccagt
     4321 gccagcggcg ccggtgtcgg gatgccgagg aactgcaggc gatgcgcttc gcgccacgag
     4381 tgccaggccc ggctcggtcg ccagcagcgg ttcagggcgt gccccaggct cttcaggttg
     4441 taacgcttga gcagcagcac ccgctcgtcg accacgatcc tggccaccgt gcaggtgccg
     4501 ccgtccttga gcaccacggc gctgtcgagc gcggcatccg gggccgccag cagggcggcc
     4561 agcgcgtcgg cctggcggcg cagcacggcc gtgaaccggg tcagcgtgtg ttcgaccgca
     4621 aacagcgtgc agtcgcgcac ggtttttccg agaaagtccc ggaggcgcca ggcccgcacg
     4681 tgcgcgatcc gggcgcgcag ggcctgcgga tcgggaaagg cgctcgccgc ggcctctcca
     4741 tagggggctt gcaggatgct cagccgctcg tcccatgcgg aaggcagttg cgccagcagc
     4801 accgccagat tgtcgcaggc gcgtgccatc ggcagcggct gcccgcggcc gacgcggcgc
     4861 agcgcgtcgc cgtcgatgac cagtacttct ccggcgtgat gcaggaagtt gccgagatgc
     4921 aggtcttcct ggaccaggcc ggcggcgtgc aggcggccca gcaggcgcac agccggtgcc
     4981 agcacggcca gggcggcggc gtcatccggc gactgccgcg gcaggcgcgc ccacgcctcg
     5041 gcaagggtct cggcgggatc gaggaaggcg gtcagcagtg cgtggccgcc tccggccagc
     5101 ggcgtcgcgg cgagcagcgc cggggtcggt acggcggccg cggccagtgc cgcgaggccg
     5161 gtgcgttcct gccgccagtg tttcgcgctg cgtcgtccga cgaagagctt ggcgaggacg
     5221 cggcagccat cgatttcggc ttcaccgacc agccgcttgc ccggcagcac gcgcagcaga
     5281 cggcgcatgc cgagctgtcg gccgtcggcc agggcgatcc ggaaggggag cccgggatcg
     5341 cggccggcgg cgcgcagggt ggcggcatcg accagggcgg ggaactcgtc gccagggcgt
     5401 gctcccggtc ccggtgcgtg cggcttagtc gcggccattg aagaagttca cgattttgcc
     5461 gatgcgccgg cggtcgtcgg cggtggcgcg cgcgtgttgc gtgtaatcca ggtagaaacg
     5521 cagccgctgg ctgcgcgaca ggcacttcct gccgaccttg tcgaggcagg cgagatcctt
     5581 gatcatccgg tagtcgagca gcgcgccgcg gtagaagccg ccgcagggac aatcgatcag
     5641 gtaaaccgtc ggttgggcgc cgccggagac cagcaggttg cgccacttga gatcgttgtg
     5701 cgcgaaacgt gccgcgtgca gggtgcgcgt gatgtcggcg acctgccggg aaacctgttc
     5761 gacccagcgt cggtcgcgca ggcgcgggtc gccggcccgc gccattccgg ccagatcgag
     5821 cgtgtcggga atttcctcgg tgaccagcgc gccacggctg aagccgccgc agcggcgttc
     5881 caggccgtag gcgaccagcg tcgccgtcgg aattccccag gcgcggaagg ccagcaggtt
     5941 ctgccattcg gcgcgcaccc gcggcggacc gagccagcgg cgcaggccga accagcggcg
     6001 cagcgggctt ttcccggtgc cgctgtagca tttgacgtaa taacgcttgc cggcagcgct
     6061 gacacgcagc acgcggctca gcgaatcact ggtgatccgc tcgccggcga gcgcaaagac
     6121 gcgatcgata tcgccgaaca gggctgccgc cgaggcgtat tgcggattca tccaccagtc
     6181 gatcatgtca tttcaccggc ggccgacttg cgcgcgaaac gcgcctgcag gcgctgtgcc
     6241 tcgctcgcca gcctggcgag caggggcgcc tcgtcgtgca gcacgcgccg cagcggctgg
     6301 gcgaaatacg cggagagaaa acgcagccgg tcatggcggg tgaagggcat gtcgagtacc
     6361 gaaaaataca gggcggcgag gtccttgtcg cgccagcggc gcggcgtgat gtcccggacc
     6421 tgcgcccggt gcaggtcgat cagcgacagc cgcagccggt cggcagtcgg tggtggttgg
     6481 agatgcagga ggaaatggca gatgtagaag tcccgatggt tcacgccacc caggtgcatg
     6541 cgccgcgccg cctgcgccac ggcaaccagc agcgcgcgct tgaggcgcgg cgacggggga
     6601 tgttccgccc agcccaggca gtaatcctcg agactgatcg tcggcaccag ttcctcggta
     6661 atgatgaacg acagttgctg cgccggattg cggccgcgca gtccgaaggc gacagcgcgc
     6721 atgctgtcca ccccgagttc gcgcaggcgc tggatcgcca gccactcgtt cgccgccccg
     6781 agcacgggca ggcgcagcga ggcgaggttc ttggcgattt cggcccagcc gatgccgcga
     6841 tggatcttca cgaagtagcc gcgcccggcg acctcggtgc gcagcgtgcg tcgcccttcc
     6901 agctcgcggt agacctcgcc ggcgagcgcc tcgacggcaa cgaacgggtc cctgccggcc
     6961 cacaacgagc ggaacggttc gtcgagaaag actt
//
LOCUS       DAOSIT010000042         6985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9815_length_6985_cov_8.420491, whole genome shotgun sequence.
ACCESSION   DAOSIT010000042 DAOSIT010000000
VERSION     DAOSIT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_6985_cov_8.420491"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="pglX"
                     /locus_tag="PLL14_01980"
     CDS             complement(<1..1361)
                     /gene="pglX"
                     /locus_tag="PLL14_01980"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HOG02716.1"
                     /translation="MIDAKLLLADLTRLLKRLEDDLRERALSPASELPQLRGQLQAEW
                     QAARDAQRTAETFESWAEQLITQAGVHWLLSGVFLRFIEDNELVERPWLGGTPQSGRL
                     ALARDRHDAYFRQHPHENDRDYLLACFHEAGALPGLHTFFDAAHNPVFRLGISGDAAM
                     AVMQFWQEVAADSGVLRRDFSDPTWNTRFLGDLYQDLSEATRKRYALLQTPEFVEEFI
                     LDRTLTPAIHEFGYREVRMIDPTCGSGHFLLGGFQRLVEQWSNNEPGRNRRDIAQQAL
                     NAVAGVDLNPFAVAIARFRLLLAALQASDVQRLAGAPDFRIQVAIGDSLLHGRRFGLT
                     ATEDMFQGAEHFTDTGLAHAYASEDLAEVQRILGRQYHAVVGNPPYIVVRDAALNAAY
                     RRQYASCHMKYSLGAPFTERFFELAITGRGAQPAGFVGLITTNSFMKREFGAKLIEQV
                     LPRL"
     assembly_gap    1385..1484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1639..5871)
                     /gene="pglW"
                     /locus_tag="PLL14_01985"
     CDS             complement(1639..5871)
                     /gene="pglW"
                     /locus_tag="PLL14_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system serine/threonine kinase PglW"
                     /protein_id="HOG02717.1"
                     /translation="MSASRWQAVAQSQFAWEREALDWLRRHLPDREPWHAWSNFEFID
                     DEGKVNEVDALLLTPAGLFLVEIKSRPGIVSGDAHTWLWVTDGHERAYDNPLILANRK
                     AKRLASLLRRQSSVARAKVRLPFVEPLIFLSATSLKCKLAGTARSGTYQRGQPDSEAD
                     PGIIGALQNGSGGSGGIRETQASHVNPQHARVLARAMAEAGIRPSNKHRQVADYQLGA
                     LLAEGPSYPSYQDWEGTHSAMDTVRRRVRIYTVAAATTADARKSLVRQAQREFQILEG
                     IDHPGIVKVKDYQDSELGPALIFDHDRKAIRLDFLLRERGQLLSVDQRLQIVRDLAET
                     LKYAHQKRLYHRALCPQSVLVQDVAGATPRPRIMNWQTGTREGTQSSDTTDPARRTLG
                     GTLHVEAYVEDPGLVYLAPETAQAEAQHGPSLDVFSLGAIAYHVFSGRPPASSVLDLA
                     EKLRIGQGLRLSDVLDGCGKSLQDLIQYSTHPDVMGRYGSIDEFLQDLDKVVDELTTP
                     EPEATVDPAEARPEERIEGGFTVIRKMGRGSSADVLLVRPDGGSEEMVLKVALDASLN
                     ERIAAEGEALAKLRHPNIVEWQRTLTVNGRTALLMRKAGERTLSHRLREEARLSLDLL
                     QRFGEELIQAVDYLDQQGVAHRDIKPDNIGMSQAGARGKLQLVLFDFSLTRTPPENIV
                     AGTHPYLDPFLSLRKPARWDLQAERFALAVTLHEMVSGVLPYWGDGKTQAAMLDCAAT
                     LATERFDPLLRDGFTAFFARALNRNPQQRFDNAEEMLRDWRRVFEAGAMASQAPPDPF
                     EAVARLATASTNMAELGYSVEAQNVLEAMAIHNARELLAVDRVRFRYLRGVGDRIRKE
                     IRLKAKALAAIRPDLVQGRPTLHEADDDSAAAGATSVGELAAQLLPRRPAGDDRAEEA
                     ALAIYLGLEEAEGSSLWTALGVAARALSLDRNDVSAALLKARERWLKTPSLTELRNHF
                     ESLLASQGGVMSVPELASALLALRGCASPDDAERLRLATAVVRACCEAEAHLERLRFQ
                     EFEHRPWPLIAVSGELAEYARRLGASADACAEAEPLLTPQRARETLEAVPAPEASPAL
                     AAQRLLRLATAASQRAALSSRQEIYPRGMAAALALRQSLGALMGVRFLKPKDVQERVA
                     GRYPEAQPLPQRPLLDTLLAEVGALLVWRDDTPTGAGYVPSTQALGASAGTTTEYARA
                     ATAGERGWPASDTAGDAAAAAVIEAQKLEDRLAHAQRAGGFLALTVEPRLARPVEAEL
                     RRRFGRLRVSFDTLLLKAMHQQADALKVNWNLVLLADAAAPTSSDWSRLMRLVHRALP
                     QVKQTLLDATAPLLLVNCGLIARYGLMPLLDELRDEVGRPGRLASLWLLLPMAATGLP
                     TIDDVPVPVITSSQWANVPVAWARNLHRAAAA"
     gene            complement(5878..6159)
                     /locus_tag="PLL14_01990"
     CDS             complement(5878..6159)
                     /locus_tag="PLL14_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG02718.1"
                     /translation="MLRNTPRSIETPMVRSHLVELMRRHQLRIAELARLAEVNRSTVT
                     ALTRDRATRVDLGALERICTVLGCGLGDLLEIVPEEPAQDNDAPLAGTH"
     gene            complement(6649..>6985)
                     /locus_tag="PLL14_01995"
     CDS             complement(6649..>6985)
                     /locus_tag="PLL14_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02719.1"
                     /translation="SIMQNALVEPGKIYGAGTSSSFNGALTRIWNEFVTVAGLPDKYR
                     RQVNESAYALMKRLGVPQAIADGAIKHIPDLLEELGNKTPVPGGGKVLRKLSELFLNR
                     FSASTRKND"
BASE COUNT         1067 a         2174 c         2434 g         1210 t
ORIGIN      
        1 agtcgcggca gcacctgctc gatcagcttg gctccgaact cgcgcttcat gaacgaattg
       61 gtggtgatca gcccgacaaa gccggccggc tgtgcacctc gcccggtaat ggccagctcg
      121 aagaaacgct cggtgaacgg cgccccgagc gaatacttca tgtggcaact cgcgtactgc
      181 cggcgatagg ccgcgttcag cgccgcgtcg cgcacgacga tgtacggcgg gttgccgacc
      241 accgcgtggt actgcctgcc gagaatccgc tgcacctcgg cgagatcctc gctggcgtag
      301 gcgtgcgcga gtccggtgtc ggtgaagtgt tcggcgccct ggaacatgtc ctcggtggcg
      361 gtcagcccga agcgccggcc gtgcagcagg ctgtcgccga tcgcgacctg aatgcggaaa
      421 tccggcgccc cggccaggcg ctgcacgtcg ctggcctgca aggccgccag gagcaggcga
      481 aagcgcgcga tggccaccgc gaaggggttg agatcgacgc cggcgaccgc gttcagcgct
      541 tgctgcgcaa tgtcgcgccg gttgcgtccc ggctcgttgt tcgaccactg ctcgaccagg
      601 cgctggaagc cgccgagcag gaagtgcccc gagccgcagg tcggatcgat catccgcact
      661 tcgcgatagc cgaactcgtg gatcgccggc gtcagcgtcc ggtcgaggat gaactcctcg
      721 acgaactcgg gcgtctgcag cagcgcatag cgcttgcgcg tcgcttcgct gaggtcctgg
      781 tagaggtcgc cgaggaaacg cgtgttccag gtggggtcgc tgaagtcgcg ccgcagcacc
      841 ccggagtcgg cggccacctc ctgccagaac tgcatcaccg ccatcgccgc gtcgccgctg
      901 atccctaagc gaaagaccgg gttgtgggcg gcgtcgaaga aggtgtgcag gcccggcagc
      961 gcgcccgcct cgtggaaaca ggcgagcagg tagtcgcgat cgttctcgtg cggatgttgg
     1021 cggaagtagg cgtcgtgccg gtcgcgtgcc agcgccaggc ggcccgactg cggcgtgccg
     1081 ccgagccacg gccgctcgac cagttcgttg tcttcgatga agcgcaggaa gacgccactc
     1141 aacagccagt gcacgccggc ctgggtgatg agctgttcgg cccaggattc gaaggtctcg
     1201 gcggtgcgct gggcgtcgcg cgccgcctgc cactcggcct gcaattgacc acgcagttgc
     1261 ggcagctcgc tggctggact gagcgcgcgt tcgcgcaggt cgtcttcgag ccgcttgagc
     1321 aggcgggtca ggtcggcaag caggagtttg gcatcgatca tggaggcttc aggggaaggt
     1381 cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggaag gtcgcgacag
     1501 cgacgtttgc gattgtacgg gctggggtta caaggcacgg cagtgatccg tcggttttcc
     1561 cccatccccc tcccagcctc ccccttgaag ggggaggagt tccatgggcg atagggagtg
     1621 aattatttcc ttgtccattc aggccgcggc ggcgcggtgc aggttcctgg cccaggcgac
     1681 gggaacgttc gcccactggc tggaggtgat caccggcacg ggcacgtcgt cgatggtcgg
     1741 caggccggtg gccgccatcg gcagcagcag ccacaggctg gcgagccggc ccggccggcc
     1801 gacttcgtca cgcagttcgt cgagcagcgg catcaggccg tagcgggcga tcagtccgca
     1861 attgaccagc agcagcggcg cggtggcgtc gagcagcgtc tgcttcacct gcggcaaggc
     1921 tcggtgcacc aggcgcatca ggcggctcca atcgctgctg gtgggggcgg cggcgtcggc
     1981 cagcagcacg agattccagt tgaccttcag cgcgtcggcc tgctggtgca tggccttgag
     2041 cagcagcgtg tcgaagctca cgcgcaggcg gccaaagcgg cgtcgcagct cggcttcgac
     2101 agggcgcgcg aggcgcggct cgacggtcag cgcgaggaag ccgccggccc gctgcgcatg
     2161 cgccaggcga tcttcgagct tctgcgcctc gatgaccgcg gcggcggccg catcgccagc
     2221 cgtgtccgac gctggccagc cgcgctcgcc ggcggtggcc gcgcgcgcgt attcggtggt
     2281 ggtgccggcc gacgcaccga gcgcctgcgt cgacggcacg taacccgcgc cggtcggcgt
     2341 atcgtcgcgc cagaccagca gcgcgccgac ctcggcgagc agcgtgtcga gcagcgggcg
     2401 ttgcggcagc ggctgcgctt ccgggtagcg gccggcgacg cgctcctgca cgtccttggg
     2461 cttgagaaag cgcacgccca tcagcgcgcc gagcgactgg cgcagcgcca gcgccgcggc
     2521 catgccgcgc ggatagattt cctgccggct ggagagcgcc gcgcgctgcg atgcggcggt
     2581 ggcgaggcgc agtagccgct gtgccgcgag tgccgggctg gcttccgggg ccggcaccgc
     2641 ctccagcgtt tcgcgggcgc gctgcggcgt cagcaggggt tcggcctccg cgcaggcgtc
     2701 ggctgacgcg ccgagccggc gggcgtattc ggccaactcg ccggaaacgg cgatcagcgg
     2761 ccacgggcga tgctcgaact cctgaaaacg caggcgttcg aggtgagctt cggcctcgca
     2821 gcaggcgcgc acgacggcgg tcgccaggcg caggcgttcg gcgtcgtccg gcgacgcgca
     2881 gccgcgcagg gcgagcagcg ccgacgccag ttcgggcacg ctcatgacgc cgccctggct
     2941 ggcgagcagg ctctcgaagt ggttgcgcag ttcggtgagc gacggcgtct tcagccagcg
     3001 ttcgcgggct ttcagcaagg cggcggaaac gtcgttgcga tcgagcgaca gcgccctggc
     3061 ggccacgccg agcgctgtcc agaggctcga accctcggct tcttcgaggc cgaggtagat
     3121 ggccagggcg gcttcttcgg cacggtcgtc accggctggc cggcgcggca gcaactgcgc
     3181 cgccagttcg cccacgctgg tggcgccggc cgctgccgaa tcgtcgtcgg cttcgtgcag
     3241 cgtcggccgg ccctgcacga ggtcgggtcg gatggcggcc agggctttgg ccttgaggcg
     3301 gatttccttg cggatgcggt caccgacgcc gcgcaggtag cggaagcgga cgcgatcgac
     3361 ggccagcaac tcgcgtgcgt tgtggatggc catcgcttcg aggacgttct gcgcttcgac
     3421 gctgtagccg agttcggcca tgttggtact ggcggtggcc aggcgggcga cggcttcgaa
     3481 ggggtctggc ggcgcctgac tggccatggc gccggcctcg aagactcggc gccagtcacg
     3541 cagcatctct tcggcgttgt cgaagcgctg ctgtgggttg cggttcagcg ctctggcaaa
     3601 gaaggcggtg aagccgtcgc gcagcagggg gtcgaagcgc tccgtcgcca gcgtggctgc
     3661 gcagtcgagc atcgcggcct gcgtcttgcc gtcgccccag tacggcagca cgccgctgac
     3721 catttcgtgc agcgtgacgg cgagggcaaa gcgctcggcc tgcagatccc agcgcgcggg
     3781 tttgcgcagc gagaggaagg ggtccagata cggatgcgtg ccggcgacga tattctctgg
     3841 cggggtgcgg gtgagcgaga aatcgaacaa cacgagttgc aacttgcctc tggccccggc
     3901 ctgcgacatg ccgatgttgt ccggcttgat gtcgcggtgg gcaacgccct gctggtcgag
     3961 gtagtcgacc gcctggatca gttcttcgcc gaagcgttgc aggaggtcga gcgacaggcg
     4021 ggcttcttcg cgcagccgat gcgacagtgt ccgctcaccg gccttgcgca tcagcaaggc
     4081 cgtgcgacca ttgacggtga gcgtgcgctg ccactcgacg atgttcggat gacgcagttt
     4141 cgcgagtgct tcgccctcgg cggcgatgcg ctcgttgagg ctggcgtcga gggcgacctt
     4201 gagcaccatt tcttcgctgc cgccgtccgg gcgaacgagc agtacatcgg ccgacgagcc
     4261 gcggcccatc ttgcgaatga ccgtgaagcc gccctcgatg cgctcttcgg gtctggcttc
     4321 ggccgggtcg acggtggcct cgggttccgg ggtcgtcagt tcatcgacga ccttgtcgag
     4381 atcctgcagg aactcgtcga tgctgccgta gcgacccatc acgtcgggat gggtgctgta
     4441 ctggatgaga tcctgcaatg acttgccgca accgtccagc acatccgaca gacgcaggcc
     4501 ctgcccgatg cgcagctttt cggcgagatc gaggaccgag cttgccggcg gccggccgga
     4561 gaacacgtga taggcgatcg cgcccaggct gaaaacatcc aggctggggc cgtgctgcgc
     4621 ctcggcctgc gcggtttccg gagccaggta gaccaggccg ggatcctcga catacgcctc
     4681 gacgtgcagg gtgccgccaa gcgtgcggcg tgccgggtcg gtcgtgtcgc tgctctgcgt
     4741 gccctcgcgc gtaccggtct gccagttcat gatgcgcggt cgcggcgtgg cgccggcaac
     4801 atcctggacc aggacgcttt gcgggcacag ggcacggtgg tacaggcgct tctggtgggc
     4861 gtacttgagc gtctcggcga ggtcacggac gatctgcagt cgctggtcga cgctgagcag
     4921 ttgaccccgc tcgcgcagca ggaagtcgag ccggatcgcc ttgcggtcat gatcgaagat
     4981 gagtgccggg ccgagttcgc tgtcctggta gtccttgacc ttgacgatgc cggggtggtc
     5041 gatgccttcg aggatctgga actcgcgctg cgcctggcga accagcgact tgcgggcgtc
     5101 ggcggtggtg gcggcggcga ccgtgtagat gcgaacgcgc cgacgtacgg tgtccatggc
     5161 gctgtgcgtg ccttcccagt cctgatagga cgggtacgac ggaccctcgg cgagcagtgc
     5221 gccgagctgg taatcggcca cctggcgatg cttgttcgac ggtcggatgc ccgcctcggc
     5281 catggcgcgg gcgagcacgc gcgcgtgctg tgggttgacg tggctcgcct gggtctcgcg
     5341 gatgccgccg ctgccgccgc tgccgttttg cagcgcgccg atgatgccgg ggtcggcttc
     5401 gctgtccggc tgaccgcgct ggtaagtccc tgagcgtgcc gtgccggcga gcttgcactt
     5461 gaggctggtc gccgacagga agatcagcgg ctcgacgaag ggcaggcgca ccttcgccct
     5521 ggctaccgag gactgccgcc gcagcaggct ggcaagccgc ttcgccttgc ggttggcgag
     5581 gatcagcggg ttgtcgtaag cgcgttcgtg gccatcggtg acccacaacc aggtgtgggc
     5641 gtcgccgctg acgataccgg gccggctctt gatctcgacg aggaagaggc ctgccggcgt
     5701 caggagcagc gcgtcgacct cgttgacctt gccttcatcg tcgatgaatt cgaagttgct
     5761 ccacgcgtgc cagggctcgc gatcgggaag gtggcggcga agccagtcaa gcgcctcgcg
     5821 ctcccaggcg aactgcgact gggcaacggc ctgccagcgg ctggcgctca tcggcattca
     5881 gtgcgttccg gcaagcggcg cgtcgttgtc ttgtgccggc tcctcaggga cgatctccag
     5941 caagtcgccc aagccgcagc cgaggaccgt gcagatccgc tccagcgcgc cgaggtcgac
     6001 ccgggtcgcc cgatcgcgcg tcaaggcagt caccgtgctc cgattgacct ctgccaggcg
     6061 cgccaactcc gcgatcctga gctgatggcg gcgcatgagt tcgacgagat gagaacggac
     6121 catgggggtc tcaattgatc gcggggtgtt gcgcagcata gcacgcattg ctgggcgacg
     6181 atgatttggg tatgaggcca agaacttccc catcgacttg cccgatccgg ttgaggctat
     6241 ccagttccgt atggaacaag ggttcaacga cttgttcgat ccgggcaggc tgttcgcaaa
     6301 tctgcagcag tcttgttcat gaacttgatg cagtgaattc aagccatctt ccctgcggga
     6361 aagaaacgac ctgttctcct gaattgccat gcagggggac atgggtatct gtgtttatct
     6421 gtcagtagag acgaggcact gtttgccaaa tctgatacac accccatgct ccatccccgc
     6481 cggatcgtgg cgtgcgcgat cgaagggcga tggtccaatc atcgccgcat ggcagtcaga
     6541 aagttggtga aacctgctca atgtgaatgg tgcgtcaggg tatggtgtca ggccgggcgc
     6601 aatcagcaaa cgctgtctca cctgcgccaa gtcttatatt cccgtcagtc agtcattctt
     6661 gcgagtgctg gcggaaaacc tgttgagaaa taattccgag agctttcgca agacctttcc
     6721 gccgccggga accggcgtct tgttgcccag ttcttcgagg agatccggta tgtgcttgat
     6781 cgcgccatcg gcaattgcct gcggtacgcc aagacgcttc atcagggcat aggccgattc
     6841 gttgacctgg cgtctgtact tgtcgggtaa cccggcgacc gtcacgaact cattccagat
     6901 gcgggttaac gctccattga acgaactgct ggtgccggca ccgtagatct tgcctggttc
     6961 aacaagtgcg ttttgcatga tcgat
//
LOCUS       DAOSIT010000043         6984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9820_length_6984_cov_6.687545, whole genome shotgun sequence.
ACCESSION   DAOSIT010000043 DAOSIT010000000
VERSION     DAOSIT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9820_length_6984_cov_6.687545"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1175
                     /locus_tag="PLL14_02000"
     CDS             <1..>1175
                     /locus_tag="PLL14_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HOG02720.1"
                     /translation="LSGRYPMAASLEQFWANLRDGVDCITEVPAERWDHSRYYHPDRS
                     RQGATNSKWGGFLDSVDQFDPLFFNISPRDAEIIDPQERLFLEGVYHAIEDAGYTRHS
                     LTESGNNVGVYVGVMYEEYQLYGAEQTALGRPMALSGSPSSIANRVSYFFDLNGPSMA
                     VDSMCSSSLTAIHLACQALQQGDCSMAIAGGVNLTLHPNKYLALGQGRFASSQGRCKS
                     FGEGGDGYVPSEGVGVLLLKPLAQAIADGDHIYGVVKGSAINHGGKTNAYTVPNPQAQ
                     AGVIARAYARAGIAPRTVSYVEAHGTGTALGDPIEIAGLSSSFRDGTPDTGFCAIGSV
                     KSNIGHCESAAGVAGVTKVLLQLKHRQLAPSLHAATLNANIDFDSTPFVVQQHLGPW"
     assembly_gap    1176..1275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1276..>6984
                     /locus_tag="PLL14_02005"
     CDS             <1276..>6984
                     /locus_tag="PLL14_02005"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG02721.1"
                     /translation="EKPRSDVLLQVVVPLDGEGALFAALAAMLKTAQLENPRLRTQLI
                     AISGSEDLAEILQTESAHPHVPQVRYRAGRRQVLEHVELPPAPVLAHPWRDKGVYLIT
                     GGAGALGLIFARDILRQAPRATVILSGRAAAVSLPASAAGMVYRQMDVADAEVVERML
                     ASIGREFGSLTGILHSAGVLRDSYLLKKSRADLDAVLAAKVRGLVNLDLASRALPLEL
                     FVAFSSLAGVTGNPGQADYASANSFMDHYVAYRNTLVTRGERAGRSLALNWPLWAEGG
                     MRPDPASVQLMRDRFGLSPLASESGIAAFYQALASGHDQVLIAAGERGRLLDLFDARG
                     VQPGSDAAALLPEAACDLGDRVRAMLRQHAADLIKVGTEVIEPDVALSEYGYDSVTMT
                     ELANKLNDAYDLELTPTLCFEYPSIAAMAGYLLGAHPAVFARRFAPRTVPPRQPPAQR
                     ERPPAAARPAPLPGASAPIAVVGISGCMPMAVDLDAFWANLVAERDCISEIPASRWDW
                     KALDGDPEREPNPSNVRWGGFIDGIDEFDPLFFGISPREAEVMDPPQRLLMMHVWKAL
                     EDAGHAPESLSGSRTALFVGTGASGYHTLFSATQRSMEGYSATASVPSIGPNRMSYLL
                     NLRGPSEPIETACSSSLVAVIRGVSVLRDGSCDLAIVGGINTIITPEAHISFNKAGML
                     SPDGRCKPFAQQANGYARGEGVGMLVLKRLSDAEAAGDHIHGVILGCAENHGGRATSL
                     TAPNPQAQAELLRDAYRRAGIDPRTISYVEAHGTGTRLGDPVEIAGLKAAFGESGEQG
                     FCGLGSVKSNIGHLELAAGVAGMLKVLLQMRHKTLVKSLHSEQLNPYFDLADSPFYVV
                     QQTAPWLAQRDAQGHPLPRRAGVSSFGFGGVNAHVLLEEYIPSPPPVVTNRPALIVLS
                     ARNEERLLEVVSNLLAFLNRAAEPGPAWLASLACTLQLGRDAMEERLAWSVTTVTELI
                     GNLQAFVARGALGGGMRGQVRRHKEALSVLAADEDAAALIDAWVAKGKTLRLLELWTK
                     GLHVGWERLYGAGMPPRLRLPTYPFARERYWAAPDATRPAAAGGSLLHPLLHANTSDF
                     NAQRFTSRFSGQESFLADHRVQGQRVLPGVAYLEMARAAISHSLRDCPGLVLNHVVWA
                     RPFVAGDTPPPPLDVVLSEQNDGRIAFAIQSAAEEAVIHCQGVAEVSASGEPAAVLDL
                     PAVQQRMLRGQLSGAQCYGAFAGMGIDYGPGHRAIAALHVGAGEVLARLSLPSLGAGF
                     VLHPGMLDAAIQSSLGLLLDDHGRLDGAAAMLPFAVKRVDIATPCTTEMWAVVATAGD
                     GLDMALCDLDGRVCVRLSGFRTRPVEASAAPRPPGSVVEALAGELHGQVRAALRQMVS
                     ALIKVNPTDIDLDSELSEYGFDSISLTELANRLNSEFDLELMPTLFFEFPSIVALAAH
                     LVEAHPVVFARRFGAAAATPTALPPSTAALRSRRRHRTAAVSADTASEPIAIVGMSAC
                     LPMAADVDAFWDNLLAGRDCISEIPASRWDWRAVYGDPGREANRTNVKWGGFIDGVDQ
                     FDPLFFGITPREAEVMDPQQRLLMTHVWKAIEDAGYAAQTLSGSRTALFVGTGASSYG
                     SLLARSPRAHDAFSSTAVVASMGPNRMSYFLNLHGPSEPIETACSSSLVALNRGVAVL
                     RDGSCDMAIVGGVNTMVTPDAHISFSKAGMLSPDGRCKTFSPQANGYVRGEGVGMLVL
                     KRLSDAEGAGDPIHGLIRACAENHGGRASSLTAPNPLAQAALLRETYARAGIDPRSIG
                     YIEAHGTGTRLGDPVEINGLKNAFGELGAQAFCGIGSVKSNIGHLELAAGVAGVIKVL
                     LQMRHKTLVKSLHSEHISPYIELAGSPFYVVQETTPWLAQRDAQGRELPRRAGVSSFG
                     FGG"
BASE COUNT         1103 a         2285 c         2339 g         1157 t
ORIGIN      
        1 ctgtcggggc gctatccgat ggccgccagc ctggagcaat tctgggccaa cttgcgcgac
       61 ggcgtcgatt gcatcaccga ggtcccggcc gagcgctggg atcacagccg gtactaccac
      121 ccggacagga gccggcaggg ggcgaccaac agcaaatggg gcggcttcct ggacagcgtc
      181 gatcagttcg atccgctatt cttcaacatc tcgccgcgcg acgccgagat catcgacccg
      241 caggagcgcc tgttcctcga aggggtctac cacgccatcg aagacgccgg ctacacccgc
      301 cacagcctga ccgagtcggg caacaacgtc ggggtctacg tcggcgtgat gtacgaggag
      361 taccagctct acggcgccga acagaccgct ctgggcaggc cgatggcctt gtccggcagc
      421 ccgtcgtcga ttgccaaccg ggtttcgtat ttcttcgatc tgaacggccc gagcatggcg
      481 gtggacagca tgtgttcgtc gtccctgacc gcgattcatc tggcctgcca ggccttgcag
      541 cagggcgatt gctcgatggc catcgccggc ggtgtgaacc tgacgctgca tccgaacaag
      601 tatctggcgc tggggcaagg ccggtttgcg tcgagccagg gccgctgcaa gagtttcggt
      661 gaaggcggcg acggctatgt accgagcgaa ggcgtcggcg tgctgctgct caagccgctg
      721 gcgcaggcca tcgccgatgg cgaccacatc tacggcgtcg tcaagggaag cgccatcaac
      781 catggcggca agaccaatgc ctataccgtg ccgaatccac aagcccaggc cggcgtcatc
      841 gcccgcgcct acgccagggc tggcatcgcc ccgcgcaccg tcagctacgt cgaagcgcac
      901 ggtaccggca ccgcgctggg agatccgatc gagatcgccg ggctgagcag cagcttccgc
      961 gacggcacgc ccgataccgg attttgcgcc atcgggtcgg tcaagtcgaa catcggtcac
     1021 tgcgaaagcg cggccggcgt tgccggcgtg accaaggtgc tgctgcagct caagcaccgc
     1081 caattggcgc cgtcgttgca tgcggccacg ctcaacgcca acatcgattt cgacagcaca
     1141 ccgttcgtgg tgcagcagca cctgggtcca tggcannnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnggaga agccgcgcag cgatgtgctg ctgcaggtgg tggttccgct
     1321 cgacggcgag ggggcactct tcgcggcact ggcggcgatg ttgaagaccg cgcaactgga
     1381 aaatccgcgc ctccggacgc agttgatcgc gatttccgga tcggaagatc tcgccgagat
     1441 tctgcaaacc gaaagcgcgc acccgcacgt gccacaggtg cgctatcgcg ccggccgccg
     1501 acaagtcctg gaacacgtcg aactgccgcc ggcccccgtg ctcgcgcatc cctggcgcga
     1561 caagggggtc tacctcatca ccggcggtgc cggtgccctg ggcctgatct tcgcccgcga
     1621 catcctccgc caggcgccgc gtgccaccgt gatcctgagc ggccgagcgg cggcggtgtc
     1681 gctgccggcg tccgccgccg ggatggtcta ccgccagatg gacgttgccg acgccgaggt
     1741 ggtggagcgc atgctggctt cgatcggtcg tgaattcggc agcctgacgg gcatcctgca
     1801 cagtgccggg gtgttgcgcg acagctacct gctgaagaag agccgtgccg atctggatgc
     1861 cgtgctggcc gccaaggtgc gcgggctggt gaatctcgat ctggccagtc gcgccttgcc
     1921 gctcgaactg ttcgtggcct tctcctcgct tgccggggtg accggcaatc cgggacaagc
     1981 cgactatgcg agcgccaatt ccttcatgga ccactacgtc gcttaccgca ataccctggt
     2041 cacgcgcggc gagcgcgctg gccgcagcct ggcgctgaac tggccgttgt gggctgaggg
     2101 cggcatgcgc cccgaccccg ccagcgtgca gctgatgcgc gatcgcttcg gtttgagccc
     2161 gctggccagc gaatccggca tcgcggcgtt ttaccaggcg ctcgccagcg gccatgacca
     2221 ggtgctgatc gccgccggcg agcgtggcag gctgctcgat ctgttcgacg cccggggcgt
     2281 gcagcccgga tccgacgcgg cggcgctcct gccggaagcg gcgtgcgatc ttggcgacag
     2341 ggtgcgtgcc atgctgcgcc agcacgcggc ggatctgatc aaggtcggaa ccgaggtgat
     2401 cgagccggac gtcgcgctca gcgaatacgg ctacgactcg gtaacgatga ccgagctggc
     2461 gaacaagctc aatgacgctt acgacctgga actcacgccg acgctgtgct tcgagtatcc
     2521 gagcattgcg gcgatggccg gttacctgct cggcgcgcat ccggcggtgt tcgcgcgccg
     2581 cttcgcgccg cgcacggtgc cgcctcgcca accgcccgca cagcgggaac gcccgccggc
     2641 agccgcgcgg ccggcaccgc tgcccggcgc atcggcgccg atcgccgtcg tcggcatcag
     2701 tggctgcatg ccgatggccg tcgatctcga tgccttctgg gccaatctgg tggccgaacg
     2761 cgactgtatc tcggaaattc cggcaagccg ctgggactgg aaggccctgg acggcgatcc
     2821 cgagcgcgaa ccgaacccgt cgaatgtcag gtggggcggt ttcatcgatg gcatcgacga
     2881 gttcgatccg ctgttcttcg gcatctcgcc gcgcgaggcc gaagtgatgg acccgccgca
     2941 gcggctgttg atgatgcatg tctggaaggc gctggaagac gccgggcatg cgcccgagag
     3001 cctgtccggt agccgcaccg cgctgttcgt cggcaccggt gcgagtggct atcacaccct
     3061 gttttcggcg acccagcggt cgatggaagg ttacagcgca accgccagcg taccgtcgat
     3121 cgggcccaac cggatgagct atctgctcaa tctgcgcggc cctagcgaac cgatagaaac
     3181 cgcctgttcg agttcgctgg tggcggtcat ccgtggcgtg tccgtgctgc gcgacggcag
     3241 ctgtgacctg gcgatcgtcg gcggcatcaa caccatcatc actccggaag cgcatatcag
     3301 tttcaacaag gccggcatgt tgagcccgga cgggcgctgc aagcctttcg cgcaacaggc
     3361 caacggctat gcgcgtggcg agggcgtcgg catgctggtg ctcaagcgcc tgtcagacgc
     3421 cgaggcggcg ggcgaccaca tccatggcgt gatccttggc tgcgccgaaa accatggtgg
     3481 gcgcgccacc tcactgacgg caccgaatcc gcaggcgcag gccgaattgc tgcgcgacgc
     3541 ctaccgccgc gccggcatcg acccgcgcac gatcagctat gtcgaggcgc acggtaccgg
     3601 tacgcggctc ggtgacccgg tcgagatcgc cgggctgaag gcggctttcg gcgagtcggg
     3661 cgagcagggg ttctgcggac tcggctcggt gaagagcaac atcggtcatc tggaactggc
     3721 cgccggcgtc gcgggcatgc tcaaggtgct gctgcagatg cggcacaaga cgctggtcaa
     3781 gagtctgcac agcgagcagc tcaatcccta tttcgacctg gccgacagtc cgttctacgt
     3841 cgttcagcag acggcgccgt ggctggcgca gcgcgatgca cagggccatc ccctgccgcg
     3901 ccgcgccggc gtcagttcct tcggcttcgg aggggtcaat gcgcatgtgc tgctggaaga
     3961 atatattcct tcgccgccgc ctgttgtcac caatcgaccg gcactgatcg tgctgtcggc
     4021 gcgcaacgag gagcggctgc tggaggtcgt gtccaatctc ctggccttcc tgaaccgcgc
     4081 cgccgagcct ggtccggcct ggctcgcatc gctggcctgc accttgcagc ttgggcgcga
     4141 cgcgatggaa gaacggctcg cctggagcgt gacgacggtg accgagctga tcggcaacct
     4201 gcaggctttc gttgcccgtg gcgcgctcgg cggcggcatg cgcggccagg tcaggcgcca
     4261 caaggaggcg ctgtcggtgc tggcggccga cgaggatgcc gcggccctga tcgatgcctg
     4321 ggtggccaag ggcaaaaccc tgcgcctgct cgaactgtgg accaaaggac tccacgtcgg
     4381 ctgggagcgg ctgtacggcg ccggcatgcc accgcgtctg cgtctgccga cctacccatt
     4441 cgccagggag cgctactggg ctgcgcccga cgcgacccgg ccggctgctg cgggaggatc
     4501 cctgctgcac cccttgttgc atgcgaatac ctccgatttc aacgcgcagc gcttcacctc
     4561 gcgtttcagc ggccaggagt cgtttctggc cgatcaccgg gtgcaggggc aacgtgtgct
     4621 gcccggcgtg gcgtacctgg agatggcgcg cgcggcaatc tcgcactcgc tcagggattg
     4681 cccgggcctg gtcctgaacc atgtggtctg ggcacgtccc ttcgttgccg gcgacacgcc
     4741 gccgccgccg ctcgacgtgg tcctgagtga gcagaacgac ggtcgaatcg ccttcgccat
     4801 ccagagtgcc gcagaggaag cggtgatcca ctgccagggt gtggccgaag tatcggccag
     4861 cggcgagccg gcggcggtac tggatttgcc cgccgtacag caaaggatgc tgcgcggcca
     4921 gttgagcggc gcgcagtgtt acggcgcctt tgccgggatg ggcatcgact acggacccgg
     4981 ccaccgcgcc atcgccgcgc tccacgtggg ggccggggaa gtgctggcgc gcctttcgct
     5041 gcccagcctg ggcgccggtt tcgtgctgca tccgggtatg ctcgatgcgg caatccagtc
     5101 gagtctgggc ctgctgctcg atgatcacgg ccggctggac ggcgcggcgg cgatgctgcc
     5161 gttcgcggtc aagcgggtcg acatcgccac tccctgcacc accgagatgt gggccgtcgt
     5221 ggcgaccgct ggcgacggac tcgacatggc tctgtgtgat ctggacggac gcgtctgcgt
     5281 gcgtctcagc ggtttccgca cgcgcccggt cgaggcgtcc gccgcgccgc ggccgcccgg
     5341 cagcgtcgtc gaggcgctgg cgggcgaact gcacggccag gtgcgcgctg ccttgcggca
     5401 aatggtgtcc gcgctgatca aggtcaatcc gacggacatc gatctggaca gcgagctgag
     5461 cgaatatggc ttcgattcga tttccctcac cgaactggcg aacaggctca attcggagtt
     5521 cgacctcgag ctgatgccga cgctgttttt cgagttcccg agcatcgtcg cgctcgccgc
     5581 tcatctggtc gaggcacacc cggtcgtttt cgcgcggcgt ttcggcgcgg cggcggctac
     5641 gccgacggca ctgccgccgt cgaccgcggc gctgcgcagc cggcgccggc accggactgc
     5701 cgcggtgtcg gccgacaccg cgtccgagcc gatcgccatt gtcggcatga gcgcctgcct
     5761 gccgatggca gccgatgtcg atgcgttctg ggacaatctg ctcgccgggc gcgactgcat
     5821 cagcgagata cccgccagtc gctgggactg gcgggccgtc tacggtgatc cgggacgcga
     5881 agcaaaccgg accaacgtca aatggggtgg cttcatcgac ggcgtggatc agttcgaccc
     5941 gctgtttttc ggcatcacgc cgcgcgaagc ggaagtgatg gatccgcagc aacggctgtt
     6001 gatgacgcat gtctggaagg ccatcgaaga cgccggctat gctgcacaaa ccctgtccgg
     6061 cagtcgcacc gcgctgttcg tcggtaccgg ggcgagcagc tatggcagcc tgctggcgcg
     6121 ctcgccgcgc gcgcacgacg ctttcagctc gaccgccgtg gtggcctcga tggggccaaa
     6181 ccggatgagt tacttcctca acctgcatgg cccgagcgag ccgatcgaaa ccgcttgctc
     6241 cagttcgctg gtggcgctca atcgaggcgt ggcggtgctg cgcgacggga gctgcgatat
     6301 ggcgatcgtc ggcggggtca acaccatggt cacccccgac gcgcacatca gcttcagcaa
     6361 ggccggcatg ttgagccccg atgggcgctg caagaccttc tcgccgcagg ccaacggcta
     6421 tgtacgcggc gaaggcgtcg gcatgctggt actcaagcgc ctgtccgacg cggaaggggc
     6481 gggcgacccc atccatggtc tgatccgcgc ttgtgccgag aatcacggtg ggcgtgccag
     6541 ttcgctgacc gccccgaatc cgctggcgca ggccgcgctg ctcagggaga cctatgcgcg
     6601 tgccggcatc gacccgcgca gcatcggcta catcgaggcg cacggcaccg gtacgcggct
     6661 gggcgatccg gtcgagatca acggcctgaa gaatgccttc ggtgaactcg gggcgcaggc
     6721 gttttgcggg atcggctcgg tgaagagcaa catcggtcac ctggaactgg ccgccggcgt
     6781 tgccggcgtg atcaaggtcc tgctgcagat gcgccacaag accctggtca agagtctgca
     6841 cagcgagcac atcagcccgt acatcgagct cgccgggagc cccttctacg tcgtccagga
     6901 aaccacgcca tggctggcgc agcgtgacgc gcaagggcgc gagttaccgc gccgcgccgg
     6961 cgtcagctcg ttcggcttcg gcgg
//
LOCUS       DAOSIT010000044         6977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9833_length_6977_cov_9.884282, whole genome shotgun sequence.
ACCESSION   DAOSIT010000044 DAOSIT010000000
VERSION     DAOSIT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_6977_cov_9.884282"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..85)
                     /locus_tag="PLL14_02010"
     CDS             complement(<1..85)
                     /locus_tag="PLL14_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOG02722.1"
                     /translation="MENCLFCKIVGGQIPCRKVYEDDDILAF"
     gene            complement(89..409)
                     /locus_tag="PLL14_02015"
     CDS             complement(89..409)
                     /locus_tag="PLL14_02015"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOG02723.1"
                     /translation="MNMDMLHRISDTLLERRKADPETSYTAQLLAKGPDSILKKIGEE
                     CAELIMAGKEGNRLHVVWESADVLYHTMVLLAFYGLTIEDVLQEMRRREGISGIDEKK
                     SRKS"
     gene            complement(406..810)
                     /gene="hisI"
                     /locus_tag="PLL14_02020"
     CDS             complement(406..810)
                     /gene="hisI"
                     /locus_tag="PLL14_02020"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOG02724.1"
                     /translation="MSDLNPRHACLDALKWDAQGMIPAIAQDADSGRVLMFAFMNRQS
                     LQETIDSGTAVYWSRSRQRLWRKGEESGNVQRVRSIRTDCDGDVLLLSIDQAGGIACH
                     TGRESCFFHQLQGNFWVAVDPVLKDPKDIYGK"
     gene            complement(807..1568)
                     /gene="hisF"
                     /locus_tag="PLL14_02025"
     CDS             complement(807..1568)
                     /gene="hisF"
                     /locus_tag="PLL14_02025"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOG02725.1"
                     /translation="MGLAKRIIPCLDVTAGRVVKGTNFVDLRDAGDPVEIARRYDEQG
                     ADEVTFLDITASSDQRDIILHIVEACAAQVFIPLTVGGGVRSVADVRRLLNAGADKVS
                     MNTAAVENPELVAEASAKVGNQCIVVAIDAKQSAPGVWQVFTHGGRRNTGIDAVAWAR
                     RVQQLGAGEILLTSMDRDGTRNGFDLSLTRAISDAVDVPVIASGGVGNLQHLADGVSE
                     GHADAVLAASIFHFGEFTVRQAKEYLRERGIEVRL"
     gene            complement(1609..2352)
                     /gene="hisA"
                     /locus_tag="PLL14_02030"
     CDS             complement(1609..2352)
                     /gene="hisA"
                     /locus_tag="PLL14_02030"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOG02726.1"
                     /translation="MLIIPAIDLKQGQCVRLKQGLMDQVTVFSHSPGAQASHWLAQGA
                     RRLHVVDLDGAFAGKPKNEKAIKEIVKAVGDDIPVQLGGGIRDLDTIERCLDDGVSYV
                     IIGTAAVKNPGFLHDACGAFPGHIIVGLDARDGRVAVEGWSKMTGHDVIDLARKYEDY
                     GVEAIIYTDIGRDGMLSGINIEATVKLAQALLIPVIASGGLSSLDDIRQLCAVEDEGI
                     SAAIAGRAIYDGSLDFAMAQAAADDAAGP"
     gene            complement(2404..>2811)
                     /gene="hisH"
                     /locus_tag="PLL14_02035"
     CDS             complement(2404..>2811)
                     /gene="hisH"
                     /locus_tag="PLL14_02035"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOG02727.1"
                     /translation="FLGICIGQQMLFEHSEEGDVPGLGVLRGSVRRFPADRMRDSDDH
                     KLKVPHMGWNQVRHCREHPLWAGIADGARFYFVHSYYVAPMDDDCVVGTTDYGILFTS
                     AVARDNIFAIQCHPEKSAQAGLAMLANFVAWKP"
     assembly_gap    2812..2911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2933..3520)
                     /gene="hisB"
                     /locus_tag="PLL14_02040"
     CDS             complement(2933..3520)
                     /gene="hisB"
                     /locus_tag="PLL14_02040"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOG02728.1"
                     /translation="MRQAEVSRNTLETRITVRLNLDGNGRGSFATGVPFLDHMLDQIS
                     RHGLIDLDIQATGDLHIDAHHTVEDIGITLGQALAQALGDKKGLRRYGHAYVPLDEAL
                     SRVVVDLSGRPGLEFHVDFSRAMIGTFDVDLVHEFFQGLVNHALLTLHVDNLRGDNAH
                     HQAETVFKAFGRALRMALEHDPRASDSIPSTKGSL"
     gene            3678..4754
                     /gene="hisC"
                     /locus_tag="PLL14_02045"
     CDS             3678..4754
                     /gene="hisC"
                     /locus_tag="PLL14_02045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOG02729.1"
                     /translation="MSRYWSSLVHRLTPYVPGEQPKLPNLVKLNTNENPYPPSAQVVA
                     AIADELANGGRSLRLYPDPNAERLKEAVAARYAGFGIRPQQVFVGNGSDEVLAHIFPA
                     LLKHTLPVLFPDITYSFYPVYCGLYDMAYRTVPLDEEFAIRVEDYAMANGGIIFPNPN
                     APTGCLLPLADIERLLLLHRESVVVVDEAYVDFGGDSAIALVNRHPNLLVVHTLSKSR
                     SLAGLRVGYAVGQNHLTEALERVKNSFNSYPLDRLALAGAVAAIDDQAYFELTRQAVI
                     DSREALRRALLELGFTVLPSAANFIFASHPQRDAGELAAALRQCGIIVRHFRLPRIDQ
                     YLRITIGTDAQCELLVEALRNILT"
     gene            complement(4846..5613)
                     /gene="tpiA"
                     /locus_tag="PLL14_02050"
     CDS             complement(4846..5613)
                     /gene="tpiA"
                     /locus_tag="PLL14_02050"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG02730.1"
                     /translation="MRKIVIAGNWKMNKTVAESVKLATDVVAAAQGIRSVVVVIAPTF
                     LALAKVAEVVQGSNVKLAAQDVHWENQGAFTGKVSADMLKEIGVEYVIIGHSEQRTYF
                     GETDATVNKKVRKVQSLDMTPIICIGETLAERQAGRLESVLTTQVSGAYEGLAADDAL
                     RTIIAYEPVWAIGTGVTASDDEAQQAHAFVRSLLAKRYGEDVAQSISIQYGGSMKPEN
                     AAGLLAQTDIDGGLIGGAALKADSFLGIVTPGESISK"
     gene            complement(5689..6945)
                     /gene="pgk"
                     /locus_tag="PLL14_02055"
     CDS             complement(5689..6945)
                     /gene="pgk"
                     /locus_tag="PLL14_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG02731.1"
                     /translation="MPKLFIEDLALEGKRALIRVDFNVPQDKVSGAITNTKRIEAALP
                     TIKYALDKGAAVILMSHLGRPDGKVVPQYSLAPVATALESLLGRPVTFLSDCVGPEVE
                     AACAQIKPGEVILLENLRFHIEEEGKATVTGPDGTVTKIKANPENVKAFRASLTRLAD
                     VYINDAFGTAHRDHSSMTGVQLPERSAGYLMNKELAAFSAVLESPQRPLLAILGGAKV
                     ADKIQLINNLLDKADQIIIGGGMAFTFKKVLSGMPIGNSLFDAEGAKLVPGLMDKARE
                     KGKQILLPVDFVIGDKFDAAANTGSANDVDGIPDGWLGLDCGPESTRLFSDAILQAKT
                     IIWNGPAGVFEFEKFEAGTRAMAEAVVQATAAGAITVIGGGDTATAAKKYGADKKVTH
                     SSTGGGASLEYLEGKILPGVAALSEK"
BASE COUNT         1238 a         2105 c         2112 g         1422 t
ORIGIN      
        1 gaaaggccag gatgtcgtcg tcttcataga ccttgcgaca cggaatctgg ccgccaacga
       61 ttttgcagaa caggcagttt tccatgtttc agcttttccg tgatttcttc tcgtcgattc
      121 ccgagatgcc ttcgcgccga cgcatctcct gcagtacatc ttcgatcgtc agaccataaa
      181 aagccagcag gaccatcgtg tggtagagga catccgccga ttcccagacc acgtgcaggc
      241 ggttgccttc cttgccggcc atgatcaact cggcgcattc ctcgccaatc ttcttcagga
      301 tcgaatccgg ccctttggcc agtagctgcg ccgtatagga agtttccggg tctgccttgc
      361 ggcgctcaag cagcgtgtcc gagatccggt gcagcatgtc catgttcatt tgccgtaaat
      421 atccttgggg tccttgagca ccgggtcgac cgcaacccag aagttcccct gcagttgatg
      481 aaagaaacag ctctcgcggc cggtatggca ggcgataccg cccgcctggt cgatcgagag
      541 cagcaatacg tcgccgtcac agtcggtccg gatcgagcgc acccgctgca cgttgccgga
      601 ctcttcgccc tttcgccaca gccgctggcg cgaacgcgac cagtataccg cggtcccgct
      661 gtcaatcgtc tcctgcagtg actgacgatt catgaacgca aacatcagca cccggccact
      721 atcggcgtcc tgtgcgatcg ccggaatcat accctgggca tcccacttga gggcatcgag
      781 gcatgcgtga cgagggttca gatcgctcac aggcggacct cgataccgcg ctcgcgcagg
      841 tactccttgg cctggcgaac ggtgaattca ccaaaatgga agatgcttgc cgccagaacg
      901 gcgtccgcat gcccttcgga cactccgtcg gcgaggtgct ggagattgcc gacgccgccg
      961 ctggcaatga ccgggacatc gacggcatcg gaaatcgccc gcgtcaatga cagatcgaag
     1021 ccgttcctgg tgccgtcacg atccatgctc gtcagcagga tctcgccggc accgagttgc
     1081 tggacgcggc gggcccaggc gacggcgtcg atgccggtat tgcggcgtcc accgtgggta
     1141 aatacctgcc agacacccgg cgccgactgc ttggcgtcga tggccacgac gatacactgg
     1201 ttgccaacct tggctgaggc ctcggctacc agttccgggt tctcgactgc cgcggtattc
     1261 atgctcacct tgtcggcgcc ggcattgagc aggcggcgca catcggccac cgagcgcacc
     1321 ccaccgccaa ccgtcaacgg tatgaaaacc tgcgccgcac aggcctcgac gatgtgcagg
     1381 atgatgtcgc gctgatccga ggaggccgtg atgtcgagga aggtcacttc gtcggcgccc
     1441 tgctcgtcgt agcggcgggc gatttccaca gggtcgccgg cgtcacgcag gtcgacgaaa
     1501 ttggtgccct tgacgacccg accggcagtc acatcaaggc acgggatgat gcgtttggcc
     1561 aggcccaagg tcagctaacg cctcgcgttt gccggaaggt cgcgcgcatc atggtcccgc
     1621 ggcatcgtcg gctgccgcct gcgccatggc aaaatcgagc gagccatcgt agatggcgcg
     1681 gccggcgatc gcggcgctga ttccttcgtc ttcgaccgcg cagagttgcc ggatgtcgtc
     1741 gaggctggac aggccgccgc tggcgatcac cgggatcaac aatgcctgag cgagttttac
     1801 cgtcgcctcg atgttgatcc ccgagagcat gccgtcacgg ccgatatcgg tatagatgat
     1861 cgcttcgacg ccataatcct cgtatttccg cgccaggtcg atgacatcat gtccggtcat
     1921 tttcgaccag ccttcaacag ccaccctgcc gtctctggca tcgagtccga caatgatgtg
     1981 accaggaaaa gcgccacagg catcatgcag gaaaccggga ttcttcaccg cggcagtgcc
     2041 gatgatcacg tagctgacgc cgtcgtcgag gcagcgctcg atggtgtcca aatcccggat
     2101 gccgccgccg agttgcaccg gaatgtcatc accaacagcc ttgacgattt ccttgatggc
     2161 tttctcgttt ttcggttttc cggcgaaggc gccatcgaga tcgaccacat gcaggcgccg
     2221 tgcaccctgc gccagccagt ggctggcctg cgcgccgggc gagtgggaga acaccgtcac
     2281 ctggtccatg aggccctgtt tgaggcggac gcactgtccc tgtttcaggt caatcgcggg
     2341 aatgataagc atgtcgaaag cggaagaaag gtgggttgaa acaagtcgga tcccggcaac
     2401 cggtcagggc ttccaggcaa cgaagttggc cagcatggca agtcccgctt gcgcgctctt
     2461 ttccgggtgg cactgaatgg caaagatatt atcccgcgcc actgcgctgg taaagaggat
     2521 gccataatcg gtcgttccga cgacacagtc gtcgtccatc ggggcgacgt aataactgtg
     2581 aacgaaatag aagcgtgcgc catcggcaat gcctgcccac agtgggtgct cccgacagtg
     2641 tcgtacctga ttccagccca tgtgcggaac cttgagcttg tggtcgtcgc tgtcgcgcat
     2701 cctgtccgcc ggaaaacggc ggacgctgcc ccgaaggact ccgaggcccg gaacatcacc
     2761 ttcctcactg tgctcgaaga gcatttgctg gccgatacag atgccgagaa annnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcgtgcgg gcggctcgtg catcacaggg
     2941 agcctttggt cgacggaatg ctgtcgctgg cgcgtgggtc gtgttccagg gccatgcgca
     3001 gcgcacgacc gaaggccttg aacaccgttt cggcctggtg gtgggcattg tcgccacgca
     3061 gattatcgac gtgcagggtc agcagggcat gattgacgag gccctggaaa aattcatgca
     3121 ccaggtcgac atcgaacgta ccgatcattg cgcggctgaa atcgacgtgg aactcgaggc
     3181 cgggtcggcc ggaaagatcg accacgaccc gtgacagcgc ctcgtcgaga ggcacgtagg
     3241 catgcccgta ccgccgcagg cccttcttgt cgcccagtgc ctgggccagc gcctggccga
     3301 gcgtgatgcc gatatcctcg accgtgtggt gtgcgtcgat atgcaggtcg ccggtggcct
     3361 ggatatcgag gtcgatcagg ccatggcggg aaatctggtc gagcatgtgg tcgaggaaag
     3421 gaacgccggt ggcgaagctt cccctgccgt tgccatccag attcagacga acggtgatcc
     3481 gggtttcgag cgtattcctg gaaacttctg cttgccgcat cgtgagacca ttgcagagtg
     3541 gcaaaaaact gtcaggtggg gaaaggcttc ggtttgctgc tgcgaactgt tgatcgtcgg
     3601 caggtgatgg ttctgcatcg acagagacgc atgataccat ttcaggcttg ccgtgcaccc
     3661 aacctgacct gcctttcatg agccgctact ggagttccct cgttcaccgt ctgacgcctt
     3721 acgttccggg cgagcagccg aagctgccga atctcgtcaa gctgaacacc aacgaaaatc
     3781 cctatccccc gtcggcacag gtcgtggcgg caatcgccga cgaactggcg aatggtgggc
     3841 ggtcgctgcg cctgtacccg gacccgaacg ccgaacgcct caaggaggcg gttgccgccc
     3901 gttacgctgg cttcggcatc aggccgcagc aggtatttgt cggcaacggt tccgatgaag
     3961 tgttggcgca tatctttccg gcgctgctca agcacacgct gccggtgttg ttcccggaca
     4021 tcacctatag tttctatccg gtctactgcg gcctctatga catggcctat cgtacggtgc
     4081 cgctggatga ggagttcgcc atccgcgtcg aggactatgc catggccaat ggcggcatca
     4141 tctttcccaa cccgaatgcg ccaaccggct gcctgctgcc gcttgcggac atcgagcgtt
     4201 tgctgcttct gcatcgtgag tcggtggtcg tggtcgatga agcctacgtc gatttcggcg
     4261 gtgattcggc gattgccctg gttaaccgcc atccaaacct gctggtggtg cacacccttt
     4321 ccaagtcgcg ctcgctggcc ggactgcggg ttggctatgc ggtgggccag aaccatttga
     4381 ccgaagcgct cgaaagagtc aagaacagtt tcaattcgta cccgctcgat cgcttggcac
     4441 ttgccggtgc cgttgccgcg atcgacgacc aggcgtattt cgaattgacg cgacaagccg
     4501 tcatcgacag tcgtgaggct ctgcgccgtg cgctgctgga gcttggtttc accgttcttc
     4561 cgtcggcagc caacttcatc ttcgccagcc acccgcagcg cgatgccggc gaactcgccg
     4621 ccgccctgcg gcagtgcggc atcatcgttc gccacttcag gttgccgcgt atcgaccagt
     4681 acctgcggat taccatcggc accgacgctc aatgcgaact gctggtcgag gccctgcgca
     4741 atatcctgac ttgatcggcg cgggccttgc gacgcagcca gtccgggtgg tgcggcgccg
     4801 gaaacccgcg ccgcactcac ccccttgacc gccacgccgt gcctgttatt tgctgatcga
     4861 ctcaccaggc gtgacgatgc ccaggaagct gtctgccttg agcgcggcac cgccgatcaa
     4921 gccaccgtcg atgtccgtct gagccagcag gccggccgcg ttctccggtt tcatcgagcc
     4981 gccgtactgg attgaaatcg actgggcgac gtcttctccg tagcgtttgg ccagcaggct
     5041 gcgcacgaaa gcgtgcgctt gctgcgcctc gtcgtcgctg gcggtgacgc cggtgccaat
     5101 ggcccagacc ggttcgtagg cgatgatcgt gcgcagcgca tcgtcggcgg caagaccttc
     5161 gtaggcgccg ctgacctgcg tggtcagtac gctctccagg cggccagcct gacgttccgc
     5221 gagcgtctcg ccgatacaga tgattggtgt catgtcgagc gactggacct tgcggacctt
     5281 tttgttgacc gttgcgtcgg tttctccgaa gtacgtccgt tgctcggagt ggccgatgat
     5341 cacgtactcg acgccgattt ccttgagcat gtctgcgctc accttgccgg tgaatgcgcc
     5401 ctggttttcc caatgcacat cctgagccgc gagtttgacg ttgctgccct gcaccacctc
     5461 cgcgaccttg gccagggcca ggaaggtcgg agcaatgacg acgacaacac tccggatgcc
     5521 ttgggccgct gccaccacgt cggtggcgag cttcactgat tcggcaacgg tcttgttcat
     5581 tttccagttg ccggcgataa cgatcttgcg catggtgttc ctcaatgtga tgattcctgg
     5641 gtgacgtgat gagcaaattg gccccggtga tgtcggggcc aaccgcattt atttctcgga
     5701 aagggcggcg acgcccggca ggattttgcc ttcgaggtac tcgagcgacg cgccacctcc
     5761 tgttgaactg tgggtgacct tcttgtcggc accatacttc ttcgcggcag tggccgtgtc
     5821 accgcctccg atcacggtga ttgcgcctgc agcggtggcc tggacgactg cttcagccat
     5881 ggcccgggtg cccgcttcga acttctcgaa ctcgaagacg ccggccgggc cgttccagat
     5941 gatggtcttc gcctgcagga tcgcgtcgct gaagaggcgc gtcgattccg gaccgcaatc
     6001 aagccccagc cagccgtcgg ggatgccgtc gacgtcgttg gcactgccgg tgttggccgc
     6061 ggcgtcaaac ttgtcgccga tgacgaagtc cacgggcagc aggatctgct tgcctttctc
     6121 gcgggccttg tccatcaggc cgggtacgag tttggcgcct tctgcgtcga acagggagtt
     6181 gccgatcggc attccggaga ggaccttctt gaaggtgaag gccatgccgc cgccgatgat
     6241 gatctggtcc gccttgtcga gcagattgtt gatcagctga atcttgtcgg cgaccttggc
     6301 gccgccgagg atggccagca gcggacgctg cggcgattcg agaacggccg aaaaagccgc
     6361 cagttccttg ttcatcagat agccggccga gcgttcgggc aactgcacgc cggtcatcga
     6421 cgagtggtcg cggtgagcgg tgccgaaggc gtcattgatg tagacgtcgg cgagcctggt
     6481 cagcgacgca cggaaggctt tcacgttttc cgggttggcc ttgatcttgg tgaccgtacc
     6541 gtcggggccg gtcacggtgg ctttgccttc ttcctcgata tggaaacgaa ggttctcaag
     6601 caggatgact tcgccgggct tgatctgcgc gcaggcggcc tcgacttccg gtccgacaca
     6661 gtcggagagg aaggtcactg gtctgccgag aaggctttca agagcggtgg caaccggcgc
     6721 caggctgtat tgcggcacga ctttcccgtc ggggcgcccg aggtgcgaca tgagaatgac
     6781 ggctgcgccc ttgtccaggg catacttgat cgtcggcaat gcggcctcga tgcgcttggt
     6841 gttggtaatg gcgccgctga ctttgtcctg ggggacgttg aagtcaacgc ggatcaaggc
     6901 gcgcttgcct tcgagggcga gatcttcaat gaacagtttg ggcatgattt cctcgacagt
     6961 gagtgggtgt tgaaaaa
//
LOCUS       DAOSIT010000045         6905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_9960_length_6905_cov_8.807883, whole genome shotgun sequence.
ACCESSION   DAOSIT010000045 DAOSIT010000000
VERSION     DAOSIT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9960_length_6905_cov_8.807883"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..793)
                     /locus_tag="PLL14_02060"
     CDS             complement(44..793)
                     /locus_tag="PLL14_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOG02732.1"
                     /translation="MKILVPVKRVVDYNVKVRVKSDASDVELANVKMSMNPFDEIAVE
                     EAVRLKEAGIATEVIAVSAGVLNCQETLRTAMAIGADRGILIQTDVELQPLAVAKLLR
                     AVCEKEQPQIVLCGKQAIDDDANQTGQMLAGLMNWPQATFASKIVVAGQLVTVTREID
                     GGLETVEVPLPAVLTADLRLNEPRYATLPNIMKAKRKPLETTNPAALGVDVTPRLKTL
                     KVAEPAKRTAGIKVADAAALVMKLKTEAKVI"
     gene            1013..1594
                     /locus_tag="PLL14_02065"
     CDS             1013..1594
                     /locus_tag="PLL14_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG02733.1"
                     /translation="MTMKLENARPRASLDRNGWVEAAIDMLAEQGVQGMRVEVLAKNF
                     GVTKGSFYWHFKDRQDLFTAVLETWRDGRIRDIHKQSVSTPGREHEQLLHLIDVYGAT
                     RNRRGISIELAVREWARRDAQAAAVVEEVDGWRLECTRKLFVARGVPDDEAKSRSLLL
                     YAYVFGQSLMACESYDPRIGDFKHWIAELIVKP"
     gene            1671..3182
                     /gene="glpK"
                     /locus_tag="PLL14_02070"
     CDS             1671..3182
                     /gene="glpK"
                     /locus_tag="PLL14_02070"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOG02734.1"
                     /translation="MQPLTSPAFILALDQGTSSSRAILFGRDGSIAALSQQEFTQHYP
                     QPGWVEHDAAEIWETQLTVARAVLRDHGVAANQVAAVGITNQRETTVLWDRHSGQPLH
                     RAIVWQDRRTAGLCDSLAAAGQAELFRERTGLVLDAYFSGTKLKWLLDHVPGTRARAE
                     RGELAFGTIDSWLAWKLSGGRVHVTDPSNAARTLLFDIRRRCWDEELLGLLDIPAAVL
                     PQIVDSSGLITTLDPDCLGAPIPLAGIAGDQQAATYGQACLQPGMAKNTYGTGCFLLM
                     NTGSQAMNSGHRMLTTIGWQRHEQTTYLLEGSVFMGGAIVQWLRDGLGLIASSDQIEA
                     LAASVPDSDGVFLVPAHTGLGAPYWDPYARGALLGMTRGTNRAHVARAALEAIAFQSA
                     EVLQAMELDAGGSLRELRADGGAAGNTLLLQFQADLLGVPVIRPRVTETTALGAAYLA
                     GLAVGFWQDEAELTSLWRAERQFDPTMTADQRTALLAGWRRAIERCRHWVTAT"
     gene            complement(3226..>3795)
                     /locus_tag="PLL14_02075"
     CDS             complement(3226..>3795)
                     /locus_tag="PLL14_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG02735.1"
                     /translation="SSIVDAKSHFTAAHSLGVAQLARYLGERVGLPADALAEVEVAGL
                     MHDVGKLCVPDEIVDKPAPLTPLEFSVMERHSFETYQILRHIPGLEQVAEWAAFHHEA
                     LNGHGYPFRRSAERLSPEARIIAVADIFQALAQERPYRGRQRVEQIPHFLNAFVNQGV
                     LDGDLVALACADLDGCWRAATPEVLPGSA"
     assembly_gap    3796..3895
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3896..4329)
                     /locus_tag="PLL14_02080"
     CDS             complement(<3896..4329)
                     /locus_tag="PLL14_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG02736.1"
                     /translation="MTLRIDLKSAILALSDALDLVGVNDFQHGKRVAMIARETALMLG
                     LPERRLDRLVCASLLHDLGVSSTAVHRRLLHEADWDARHTHCETGYCLLQPFAHLSQV
                     AKAIRYHHTPWVELPAGLDEADALDSNLILLGDRVDACLLQLG"
     gene            complement(4338..5159)
                     /locus_tag="PLL14_02085"
     CDS             complement(4338..5159)
                     /locus_tag="PLL14_02085"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOG02737.1"
                     /translation="MSKSIVMHLVSHASGELIEMLARYAVAQLDGVEVERRLWKVVRR
                     LDQVPEILGALAGSPGYVLHSISHGDVRAALEEGCLRLDVPCQFALEPLLGRLADYSG
                     AAVQSHNSTGDAFDEDYYHRVEAMKYTLAHDDGIGSDDLDGADVIVVGVSRATKTPTC
                     MYLASRGIKAANVPLVPGAPPPQGLLTAIGPLVVGLTVDPTRLAMVRAARLMALKDDT
                     NHDYADLDALRKEVLDARRLFLRRGWPIIDASHRSIEQTASMIIEMLRTRAGERR"
     gene            complement(5189..5941)
                     /locus_tag="PLL14_02090"
     CDS             complement(5189..5941)
                     /locus_tag="PLL14_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG02738.1"
                     /translation="MDLWACPAVVTGAASGLGAETARYLTEAGAKVTVIDIDGAGVRK
                     LADEIGAHAIPCDVADAHSAEQALAMACAVHGAARVLVHCVGRGHSEPILGAGGPIPL
                     EDFRQLVEVNLISTFNMMRLAAADMAGLPPLANGERGIILSTASVSAYDGQSGEAAYS
                     ASKGGVVSMILPAARELGPLGIRVVGIAPGLFGTQTLFDMEKNLDSRLARQIPFPHRF
                     GDPAEFAMLVLHVIKNVMINGSVLRLDGGYHR"
     gene            6178..>6905
                     /locus_tag="PLL14_02095"
     CDS             6178..>6905
                     /locus_tag="PLL14_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG02739.1"
                     /translation="MYRDQSFPVATSLFPALVFSATLVFSPLASGAAAVVKIDGSSAV
                     YPITEAVAQEFQRAKLHEVHVNVGVSGTSAGFVKFCRGETDISNASRPILKKEMEECA
                     KAGIRFYEMPLAYDALTVVVNPQNAFVSSMSVAELRKLWEPAAQGRIKTWKDLNPAWP
                     AQQIKLYAPGADSGTFESFTEVVVGTARSSRTDVSTAEDHDVLAQRIAADKDALGYLS
                     LDYYVENQKQLKAVAISDGKEAVLP"
BASE COUNT         1325 a         2228 c         2042 g         1210 t
ORIGIN      
        1 atcgaggtat tgtcgtgttc agcaataacg agaatggcca tggtcagatc accttcgctt
       61 cagtcttgag tttcatcacc agtgcggcgg cgtcggccac cttgatgccg gcagtacgct
      121 tcgcgggctc ggcaaccttg agggtcttca gacgcggcgt cacatcgaca cccagcgccg
      181 ccgggttggt ggtttcgagc ggcttcctct tggctttcat gatgttcggc aaggtcgcgt
      241 agcgcggctc gttgaggcgc aggtcggcgg tcagcacggc cggcagcggc acctcgacgg
      301 tttcgaggcc gccgtcgatt tcgcgagtca cggtcaccaa ctggccagcg acgacgatct
      361 tcgacgcgaa ggttgcctgc ggccagttca tcagtccggc cagcatctgc cccgtctggt
      421 tggcatcgtc gtcgatcgcc tgcttgccgc agaggacgat ctggggctgc tccttctcgc
      481 acacggccct gagcaacttg gcgactgcca gcggctgcag ctcgacatcg gtctggatca
      541 ggatgccgcg gtcggcgccg atggccattg ccgtgcgcag cgtttcctgg caattcagca
      601 cgcccgccga aacggcgatc acttcggtag caataccagc ctccttgagc cgcacggcct
      661 cttcaacggc aatctcatcg aagggattca tcgacatctt gacgttggcc agctcgacgt
      721 cgctggcatc ggacttgacc cgcaccttga cgttgtaatc caccacacgt ttgactggaa
      781 cgaggatttt caaatttttc tcctttatca ctaaccacag taatcgagat caagcacggg
      841 caagacaggc cgcgtttgac attgccgaat tctgaccgga caatagacca tactttatcg
      901 tatggatggc gtacggtacc gtatggagca atggctgtca acaccgcccg gcgacgcagc
      961 gacacatcaa tcagcaatat tgcctgacac gcgtgaatca ggaaaggatc cgatgacgat
     1021 gaagctcgaa aatgccagac cacgtgcttc gctcgaccgc aacggatggg tcgaagcggc
     1081 gatcgacatg cttgccgaac agggtgtgca ggggatgcgt gtcgaggttt tggcgaagaa
     1141 cttcggggtc accaagggca gcttctactg gcacttcaaa gaccgtcagg accttttcac
     1201 ggctgtcctg gaaacctggc gcgacggccg catccgcgac atccacaagc agagtgtatc
     1261 cacaccgggc agggagcatg aacagctcct gcatctgatc gacgtctatg gtgcgacacg
     1321 caaccgccga gggatctcga tcgaactggc ggtgcgcgaa tgggcacgcc gagatgccca
     1381 ggccgcagcg gtcgtcgagg aagtcgacgg ctggcggctg gaatgcaccc gcaaattgtt
     1441 cgttgcccgc ggcgttcccg atgacgaggc aaaaagccgc agcctgctgc tctacgccta
     1501 cgtctttgga cagagcctga tggcctgcga aagctacgac cccagaattg gcgacttcaa
     1561 gcactggatc gccgaactga tcgtcaaacc ctgactacag ccgctgccag aacgtccagc
     1621 gttcggcccg gactatcgac caccacgact tctcgccggg agtttgcctg atgcagccac
     1681 tcaccagccc agcattcatt ctcgccctcg atcagggcac aagcagttcc cgagccatac
     1741 ttttcggacg cgacggcagc atcgcggcgc tctcgcagca ggagttcacc cagcactacc
     1801 cgcaacccgg atgggtcgag cacgacgccg ccgagatctg ggaaacgcag ttgaccgtcg
     1861 cgcgcgccgt gctgcgcgac catggcgtcg ccgcgaacca ggtcgccgca gtcggcatca
     1921 ccaatcagag agaaacgacc gtgctgtggg atcgccacag cggccagccg ctgcaccggg
     1981 cgatcgtctg gcaggatcga cgcacggcgg gactctgcga cagcctggca gccgccgggc
     2041 aggccgagtt gttccgcgaa cgcaccggac tggttctcga cgcgtacttc tcgggcacca
     2101 aactgaaatg gctgctcgac catgtgcccg gcacccgtgc ccgcgccgaa cgtggcgaac
     2161 tggctttcgg aacgatcgac agttggctcg cgtggaaact ctccggcggg cgggtgcacg
     2221 tcaccgatcc gtcgaacgct gcacgcacgc tgctcttcga catcaggcgt cgttgctggg
     2281 acgaagagtt actgggcctg ctcgacatcc cggcagcggt acttccgcag atcgtcgaca
     2341 gcagcggcct gatcaccacc ctcgacccgg attgcctggg cgctccgatt ccactcgccg
     2401 gcatcgccgg ggaccagcaa gcagccacct atggccaggc ctgcctgcag ccgggcatgg
     2461 ccaagaacac ctacggcacc ggctgcttcc tgttgatgaa taccggcagc caggcaatga
     2521 actccgggca tcggatgctg accaccatcg gttggcaacg acacgagcaa acgacttacc
     2581 tgctcgaagg cagcgtcttc atgggcggcg ccatcgtgca atggttgcgc gacgggctcg
     2641 ggctgattgc cagttcggac cagatcgagg cactggctgc ctccgtcccg gacagcgatg
     2701 gggtgttcct ggtacccgca cataccggcc tgggcgcccc gtactgggac ccctacgcac
     2761 gcggcgccct gcttggcatg acacgcggca ccaaccgcgc gcatgtcgcg cgcgcggcgc
     2821 tcgaggccat cgccttccag agcgccgagg tgctgcaggc gatggagctg gatgccggcg
     2881 ggagcctgcg cgaattgcgc gctgatggcg gcgccgcggg caatacactt ttgttgcagt
     2941 ttcaggccga cctgctcggt gtgccggtga tccgtccgcg ggttaccgaa acgacggctc
     3001 tcggcgccgc ctacctggcg gggctggcgg taggcttctg gcaggacgaa gccgaactga
     3061 cttcactctg gcgtgcggag cgccagtttg acccgacgat gaccgcagac cagcgcactg
     3121 ccctgcttgc cggctggcga cgagccatcg aacgttgtcg ccactgggtc accgccacct
     3181 gaacgacggt gggtgccagc cccagacgca tcggatcaga ccctttcagg cgcttcccgg
     3241 caacacctct ggtgtcgccg cgcgccaaca gccgtcgagg tccgcacagg ccagcgcgac
     3301 cagatcgcca tcgagcacgc cctgattcac gaaagcattc agaaaatgtg gaatctgttc
     3361 gacgcgttgc cgcccgcggt agggacgctc ctgtgccaga gcctgaaaaa tatccgccac
     3421 agcaatgatc cgcgcctcgg gcgagagacg ctcggcactg cgccgaaacg ggtagccatg
     3481 gccattgagc gcctcgtgat ggaaggccgc ccattcggcg acctgctcaa ggccaggaat
     3541 atggcgcagg atctggtagg tttcgaagct gtgccgctcc atcaccgaaa actccagggg
     3601 cgtcagtggc gccggcttgt caacgatctc gtccggcacg cacagtttgc caacatcgtg
     3661 catcagcccg gccacctcga cctcggccag cgcatcagcg ggcagtccca cccgctctcc
     3721 gaggtaacgg gccagttgcg cgacgcccag tgaatgcgct gccgtgaagt ggctcttggc
     3781 gtccacgatg cttgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgag
     3901 ctgcagcagg caggcgtcca cccggtcacc gagaagaatc aggttgctgt cgagggcatc
     3961 cgcctcgtcc agaccagcgg gcagttccac ccacggcgta tgatgatagc gaatcgcctt
     4021 ggccacctgc gacagatggg caaagggctg cagcaggcaa taaccggttt cgcagtgggt
     4081 gtgtctggca tcccagtccg cttcgtgcag caggcgtcgg tgcacggcgg tcgaggacac
     4141 cccgagatca tgcagcaggc tcgcgcagac cagacgatcc agccgcctct ctggcaaacc
     4201 gagcatcaac gccgtctcgc gggcgatcat ggccacccgc ttgccgtgct ggaagtcatt
     4261 cactccgacc agatcgaggg cgtctgacag cgccaggatg gcgcttttga ggtcgattct
     4321 cagagtcatc gaacagtcta gcggcgctcc cccgcccgag tcctcaacat ttcgatgatc
     4381 attgatgccg tctgttcaat cgagcgatgg ctggcatcga tgatcggcca gccgcgcctc
     4441 agaaacaggc gacgcgcgtc caggacttcc ttgcgcagcg catcgaggtc ggcatagtca
     4501 tggttggtgt cgtccttcag ggccatcagg cgggccgccc tgaccatcgc caatcgcgtc
     4561 ggatcgacgg tcagtccgac gacgagcggc ccgatcgctg ttaacagccc ttgcggcggc
     4621 ggcgcacccg gtaccagcgg cacattggca gccttgatgc cgcgtgaggc gagatacatg
     4681 caggtcggcg tcttggtcgc ccgcgacacc ccgacgacaa tgacatcggc gccgtccaga
     4741 tcgtcgctgc cgatcccgtc atcgtgcgcc aaagtgtact tcatggcctc gacgcggtgg
     4801 taatagtcct cgtcaaaggc gtcaccggta ctgttgtgcg actgcacggc tgctccggag
     4861 taatcggcca gcctgccgag caagggttcc agcgcaaact ggcatggcac atcgagtctg
     4921 agacaacctt cttcaagtgc cgcccggaca tcaccgtggc tgatgctgtg caggacatag
     4981 ccgggcgagc ccgccagcgc tccgagaatt tcgggaacct ggtccaggcg acgaaccact
     5041 ttccacagcc gtcgctccac ttcgaccccg tccagttggg caaccgcgta gcgcgccagc
     5101 atctcgatca gttcacctga agcatgcgac accaggtgca tgacaatgct cttgctcatg
     5161 gcccctcctc ggtcatcaga ctcccaactc agcggtgata accaccatcc agacgcagca
     5221 cactgccatt gatcatgacg ttcttgatga cgtgcaggac cagcatggcg aactcggcag
     5281 gatcgccaaa gcggtgggga aacggaatct gcctcgccag ccgagaatca aggttctttt
     5341 ccatgtcaaa cagcgtttga gtaccaaaca gtccgggcgc gatgccgacc acacggatgc
     5401 ccagaggacc gagttcgcgg gcggccggca ggatcatgct gacgacaccg cccttggagg
     5461 ccgagtaggc cgcctcgccg ctttgtccat cgtaggccga gactgacgcc gtcgacagga
     5521 tgatgccacg ctcgccgttg gccagcggtg gcagcccggc catgtcggcg gcagccagcc
     5581 gcatcatgtt gaaggtcgag atcaggttga cctcgacgag ttgacgaaaa tcctccagcg
     5641 gtatcggacc gccagcaccg aggatcggct cggagtggcc gcgcccgacg cagtgtacca
     5701 gcacgcgcgc cgcgccatgg actgcgcagg ccatggcgag cgcctgctct gccgagtgtg
     5761 catcggcaac atcgcaggga atggcgtgcg caccgatctc gtcggcaagt ttccggaccc
     5821 ctgcgccatc aatgtcgatc accgtcacct tggcgccggc ctcggtaaga taacgcgccg
     5881 tctccgcgcc caagcccgac gctgcaccgg tgactaccgc cgggcatgcc cacaagtcca
     5941 ttgccacccc cctgacccgg ccgaccagtc cggccaacgc gcattatcgg tggtcgtggc
     6001 ggggcaagca aacgggcggg cagacctgcc ccttcctgcc ccagccgtcg aacaggacct
     6061 ctatccctac ggccgttcgg ccgttacgca aagcccctcg gactgtcatc aatccgtcac
     6121 aaacccgtca cataatctcc acgtcgtttc gacacactct agccacggag gctcgccatg
     6181 taccgcgatc agtcattccc tgttgcaact tcgctttttc ccgccctcgt tttctcggct
     6241 accctcgtgt tcagcccgct ggccagcggt gctgcggcag tggtaaaaat cgacggatcg
     6301 agcgcggtct atccgatcac cgaagccgtc gcccaggaat tccagcgcgc caaactgcat
     6361 gaagtgcacg tcaatgtcgg cgtgtcgggt accagcgccg gcttcgtgaa attctgtcgt
     6421 ggcgaaaccg acatctcgaa cgcgtcgcga ccgatcctga aaaaggaaat ggaggagtgc
     6481 gccaaggctg gcatccgctt ctatgaaatg cccctggcct atgacgcact caccgtggtg
     6541 gtcaatccac agaatgcctt tgtcagcagc atgagcgtcg ccgagctcag gaaactgtgg
     6601 gaacccgcgg cacaaggccg gatcaagacc tggaaagacc tcaacccggc gtggcctgcc
     6661 cagcagatca agctctatgc gccgggtgcc gattccggga ccttcgagtc cttcacggaa
     6721 gtggttgttg gtactgccag gtccagccgg accgatgtca gtaccgccga agaccatgat
     6781 gtgctggcgc agcgcattgc cgccgacaag gatgccctgg gttacctgag cctcgattat
     6841 tacgtcgaaa accagaagca gttgaaggca gtagccatca gcgacggcaa ggaagccgtt
     6901 ctgcc
//
LOCUS       DAOSIT010000046         6833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10098_length_6833_cov_10.005311, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000046 DAOSIT010000000
VERSION     DAOSIT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10098_length_6833_cov_10.005311"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2419)
                     /locus_tag="PLL14_02100"
     CDS             complement(101..2419)
                     /locus_tag="PLL14_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOG02740.1"
                     /translation="MRINILAFALGVGLLQIQERLPALPLLAGLLLGMGALFLLARRQ
                     PLLARTLTALICALLGCSWAALLAEQRLLEQLPAEWEGRDVQVVGVVAALPHAFERGE
                     RFEFVVESVETAGARVPRRIMLSWYHGRLEDEWREGLAIRPAERWRLTVRLKRPHGNA
                     NPHGFDYEAWLFERDLRATGYVRPRAVAQRLDDFVRQPPYALERLRDRIRRSFAAVLG
                     EAPYVGILVALAVGDQQAIPAEQWRLFRQTGVTHLMSISGLHVTMVAALFGGLFGGLW
                     RRSERLLLLLPVAQAAIAAGWLAAVAYSLVAGFAVPTQRTLYMLSVVALALWSGRNLG
                     ASRSLLLALLLVLLIDPWAVLAPGFWLSFVAVGLLFFVGTARLDVGRGWSRVIASWGA
                     TQWAVTLGTLPLLLLMFQQFSLVSPLANALAIPLVSFVIAPLALLFAVLPWPVLLHFD
                     HWLLSGLMAVLDWLAVWPVWEQPAPPLAATLLAVLGVLWLLLPRGFPGRWAGLCLVLP
                     ALFWPAARPAAGDAWVDVLDVGQGLAVVVRTARHTLLYDTGPLYSAESDAGQRIIVPY
                     LRASGIDQLDALIVTHRDKDHSGGVVAVQEAVPIARTLSSVQELPGERCIAGQSWEWD
                     GVRFAVLHPAAADYERKAARSNNMSCVLRIENGFGRVLLTSDIEARDEQALLARSPGL
                     LRSEILLVPHHGSGTSSTPEFIAAVGADEVIIPVGYRNRFQHPRADVVERYAGKRLWR
                     TDRDGAVRVRLADGGLSLLSYRAEYRRYWHGQ"
     gene            complement(2530..4551)
                     /locus_tag="PLL14_02105"
     CDS             complement(2530..4551)
                     /locus_tag="PLL14_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG02741.1"
                     /translation="MKTLFAPAIALLNRVGYTKKFAIMGALALMAIAVLLLNLFLSLH
                     RVIDSSLQERGGLQVLVPMAQVLQQLQAHRGLSAGVLNGNTAMEEQRAATEKAVAGAV
                     QAVTARLTPELAAGADWKKLQEGWQFLQSDGPGLIPRENFLAHTGLIADLLTFQKTIA
                     DNYALTNDPDIDATYLIDSLVDSLPWAIERMGQLRGLGTGVLAKQMPLVPSQQVEFTV
                     LLAGLNASVDSLRVNLTKTSRYNPGLQSSLAAVIRDLGASAEKMTGLVNQDILSGTYA
                     TPPAEYFALTTASVEKGYQEMFQTLFPTLEDLLQRRIDRVQRDLNLSIAVSVLIVLLY
                     AYVAMGLYYATIGSIDRLAEKARMIATGDLGVRVELGTRDELSLVGDSLNEMASAFGG
                     LIRSVQHGTGEVLQATKQLAGSAADINRASEQQSAAASTMAAAVEEMTVGIEQLSTSA
                     QDANRISSRAGELSADGNRVVGNVVREIERIAEVVNQSATLLAELGGRSERISIIVGV
                     IKEIADQTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTARSTQEIAGMIAAIQ
                     SGAHDAVASMNLGVSRVADGVALATQAGASIQEIGGNARQVVSRVADISKALGEQNIA
                     SSEIGRNVEQVARMAEENRAAVAGNAATAAHLERLAEGLESEVRRFRLL"
     gene            complement(4623..5309)
                     /gene="lolD"
                     /locus_tag="PLL14_02110"
     CDS             complement(4623..5309)
                     /gene="lolD"
                     /locus_tag="PLL14_02110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HOG02742.1"
                     /translation="MSEPVLACQGLSKIFRQGDTDVPVLLGVDLEIQVAERVAIVGAS
                     GSGKSTLLHLLGGLDAASGGSVQLMGHDLATIDDAQCGILRNRHLGFVYQFHHLLSEF
                     SALENVAMPLLIRRLPKAEAEARAAVVLGEVGLGHRLQHTPSELSGGERQRAAIARAL
                     VTEPACVLADEPTGNLDRQTAEGVFELMLALNRSRQTSFVVVTHDPALAGRVGRILTL
                     RDGRLQSVDD"
     gene            complement(5302..6549)
                     /locus_tag="PLL14_02115"
     CDS             complement(5302..6549)
                     /locus_tag="PLL14_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOG02743.1"
                     /translation="MALPYELLIGLRYTRAKKHNHFISFISLISMFGIALGVAALIVV
                     LSVMNGFQTELRSRILAVVSHVEISGVGGELVDWQRVAAQAAVQPQVIAAAPFVQAQG
                     MLAYGQAVRGAMVRGIQPDLEDQVADFRRHMKSGKFDALLPDRFNIILGSELARALGV
                     FVGDRVTLIAPQGVVTPAGVVPRLKTFTVVGLFEVGMYEYDSGLALIHLADAQRLYRM
                     DDRVSGVRLKLDDLFQAPRVARLLVGSLDTNAFISDWTRSHANFFRAVQIEKNMMFII
                     LSLIIAVAAFNIVSTLVMAVTDKQADIAILRTLGASPLSIMAVFIVQGALIGFIGLGL
                     GIAGGVALALNVDVVVPFIERLLGTQFLAKEVYYISNLPSELQWGDVTTITGVAFVLA
                     LVATLYPSWRAARVNPAAALRYE"
BASE COUNT         1325 a         2358 c         2112 g         1038 t
ORIGIN      
        1 ttgttgatga aaaacaggtc tgcaacagcg gccaggtcgg tccgggtggc agcgcgacgg
       61 ataccgtaca cacaaaggcc agtcacgcca ggcgtgcgga tcactgcccg tgccagtaac
      121 gcctgtactc cgctcgatac gacagcagcg aaagcccgcc gtcagccaat cgcacacgta
      181 ccgcaccgtc ccgatccgtg cgccacagcc gcttcccggc gtagcgttcg acgacatctg
      241 cgcgcggatg ctggaagcgg ttgcggtagc cgaccgggat gatcacttca tcggcgccga
      301 cagcggcgat gaattccggt gtcgacgagg tcccactgcc atgatgcgga acgagcagta
      361 tctcgctgcg cagcaggcct ggagagcggg cgagcagggc ctgctcgtcg cgcgcctcga
      421 tgtcggaagt cagcagcaca cgcccaaagc cgttttcgat ccgcagcacg caactcatgt
      481 tgttgctgcg cgcggccttg cgctcgtaat cggcggctgc gggatgcagg accgcgaagc
      541 gtacgccgtc ccactcccag gactgaccgg caatacaccg ttctccgggc agttcctgaa
      601 ccgacgacag cgtacgagcg attggcacgg cctcctgaac agcgaccacg cctcccgaat
      661 ggtccttgtc gcggtgcgtc acgatcaggg catcgagctg gtctattcca ctggcgcgca
      721 ggtagggaac gatgatccgt tgaccggcgt ccgactcggc gctgtacagc ggcccggtgt
      781 cgtaaagcag cgtgtgccgg gcagtgcgaa cgaccaccgc cagcccctgt ccgacatcaa
      841 ggacgtccac ccacgcgtca cccgccgccg gccgcgccgc cggccagaac agcgccggca
      901 ggaccaggca cagccccgcc cagcgacccg gaaaaccgcg cggcagcaac aaccagagca
      961 ccccgagcac ggcgagcaag gtcgccgcca gcggtggcgc cggctgctcc cagaccggcc
     1021 agaccgcgag ccaatcgaga acagccatca ggccggacaa cagccagtgg tcgaagtgca
     1081 gcaacactgg ccagggcagg acggcaaaca gcagcgccaa cggggcgatg acgaagctga
     1141 ccaacggaat cgccagcgca ttggcgagtg gcgaaaccag cgagaactgc tggaacatca
     1201 gcagcaacag cggcagcgtc ccgagggtca ccgcccactg cgtcgccccc caactggcga
     1261 tgacccggct ccagccacgc ccgacgtcaa gccgcgccgt gccgacgaag aagagcaggc
     1321 cgacggcgac aaaggataac cagaatcccg gtgcaagcac cgcccaggga tcgatcagca
     1381 gcaccagcaa caacgccagc agcaggctgc gactggcgcc gaggttgcgg cccgaccaca
     1441 gcgccagcgc caccacgctc agcatgtaca aggtgcgctg cgtcggcacc gcgaaaccgg
     1501 cgactaacga gtaagcgaca gcggccagcc agccggccgc gattgccgcc tgcgctaccg
     1561 gcagcaggag cagcaatcgt tcgctgcgcc gccatagccc gccgaaaagc ccaccaaaca
     1621 gagcggccac cattgtcacg tgtaaccccg agatcgacat caggtgcgtg acgccggtct
     1681 gcctgaacaa gcgccactgc tcggccggga tggcctgctg atcgccgact gccagagcaa
     1741 ccaggatgcc cacatacggc gcctcgccca gcaccgcagc aaaggatcgc cgtatccggt
     1801 cgcgcaagcg ctcaagggca tagggcggct gtcgtacgaa gtcgtccaga cgctgcgcta
     1861 ccgcgcgcgg gcgcacgtag cccgttgccc gcagatcgcg ttcgaaaagc caggcctcgt
     1921 agtcgaaacc gtgtggattg gcgttaccgt gcggacgctt caggcgcacc gtgagccgcc
     1981 agcgctcggc cggacgaatg gccagtccct cgcgccattc gtcttccagc cggccgtgat
     2041 accacgagag catgatccgc cgcggtacgc gcgcgcccgc ggtttccacc gactcgacga
     2101 cgaactcgaa acgctcgccc cgttcgaagg catgcggtag tgcggcgacg acaccgacga
     2161 cctgcacatc gcgcccctcc cactccgccg gcaactgctc cagcaggcgc tgttcggcga
     2221 gcagcgccgc ccaggagcaa ccgagcagcg cgcagatcag cgcggtcagc gttcgggcga
     2281 gcagtggctg gcgccgagcg agcaggaaca aggcgcccat gccgagcagc aggccggcaa
     2341 gcagcggcag cgccggcaaa cgctcctgga tctgaagcag acccacaccc agggcgaaag
     2401 caaggatgtt gatgcgcatc gcccattaaa gcagattacc cggaaacccg gtcaactgcc
     2461 ggccacctgc cgaaggaacc gcggcgaggt gccgctgaag cttccgcccg cctcccgata
     2521 cgcagtgact cagagcagcc gaaaacgacg cacctcggac tcaagacctt ccgcaagcct
     2581 ttcgaggtgc gctgccgtag ccgcattacc ggccaccgca gcacggtttt cctctgccat
     2641 gcgggccacc tgctcgacgt tgcggccgat ctcgctgctg gcgatgttct gctcgcccaa
     2701 ggccttcgag atgtcggcca cgcggctcac cacctgccgg gcattgccgc cgatctcctg
     2761 aatcgacgcg ccagcctgcg tggccagtgc gaccccatcc gccacacggc tgacaccgag
     2821 attcatgctc gccacggcat cgtgcgcacc actctggata gcggcaatca tgcccgcgat
     2881 ctcctgcgtc gaacgggccg tgcgctcggc cagcttgcgg acttcgtcgg cgacaaccgc
     2941 aaagccgcgg cccgactcgc cggctcgcgc cgcctcgatt gccgcgttga gcgccagcag
     3001 gttggtttga tcggcaattt ccttgatcac gccgacgatg atcgagatcc gttccgatcg
     3061 ccctcccagt tcggcgagga gggtcgccga ctggttcact acctcggcaa tccgctcgat
     3121 ctcgcggacg acgttgccga ccacgcggtt gccgtctgcc gacaattcac cggcacggct
     3181 ggaaatccgg ttggcatcct gggcactggt cgacaactgc tcgatgccga cagtcatttc
     3241 ttcgaccgct gcggccatgg tagacgccgc cgcactctgt tgctcgctgg cgcgattgat
     3301 gtccgcggcc gagccggcaa gctgcttggt ggcctggagc acctcgccgg tgccgtgctg
     3361 gacgctccgg atgaggccgc cgaaagcgct cgccatctcg ttcaggctgt cgccgacgag
     3421 gctcagttcg tcgcgcgtac ccagttcgac gcgcaccccg agatcgccgg tggcgatcat
     3481 cctggccttc tccgccagtc gatcgatgct gccgatcgtc gcgtagtaca gccccatggc
     3541 cacataggcg tagagcagca cgatcagaac cgaaaccgcg atgctcaggt tcaagtcacg
     3601 ctgtacgcgg tcgatccggc gctgcaggag atcttcgaga gtgggaaaaa gggtctggaa
     3661 catttcctgg tagccctttt cgaccgacgc ggtggtcaga gcgaagtact cggcgggagg
     3721 cgtcgcatag gtccccgaga gaatgtcctg gttgaccagc ccggtcatct tctctgccga
     3781 cgcgcccagg tcgcggatca cagccgccag cgaggactgc aaaccggggt tatagcgcga
     3841 ggtcttggtg agattgaccc gcaacgagtc gaccgaggcg ttgagtccgg ccagcaatac
     3901 ggtgaactcg acctgctgcg agggcaccag cggcatttgc tttgccagca cccccgtccc
     3961 gaggccccgc agttggccca tgcgttcaat cgcccaaggc aagctgtcga ccagcgaatc
     4021 gatcaggtag gtggcgtcta tgtcgggatc gttggtcagg gcatagttat cggcaatcgt
     4081 cttctggaaa gtcaataaat cggcgatcaa tccggtgtgc gccaggaagt tctcgcgcgg
     4141 gatcaacccg ggaccatcgc tctgaaggaa ctgccagccc tcctgtaatt tcttccagtc
     4201 ggcgccggcg gccagttctg gcgtcaatcg ggcagtcacc gcctgcacag cgccagccac
     4261 ggccttttcc gtggccgcgc gctgctcctc catcgccgtg ttgccgttga gcacccccgc
     4321 tgaaagcccg cgatgcgcct gcagttgctg cagcacctgc gccatcggca ccagaacctg
     4381 caggccgccc cgttcctgca gcgaactgtc gatcacgcgg tgcaggctca ggaagagatt
     4441 gagcagcagc acggcgatgg ccatcagagc cagggccccc atgatcgcga atttcttcgt
     4501 gtagcccact cgattgagca aggcaatggc cggggcaaac agagtcttca tggtcacagc
     4561 gccttcgagg tcgattgttc ccacagcata aaggccgcgc gtgcgcgtcc gtctggcgtc
     4621 gctcaatcgt caaccgattg cagacgtccg tcgcgcagcg tcaggattcg cccgacacgg
     4681 ccagccagcg ccgggtcgtg ggtgacgacg acgaaactcg tctgccgcga gcgattgagc
     4741 gcgagcatca attcgaaaac cccttcggcg gtctgccggt cgagatttcc ggtcggctcg
     4801 tcggccagca cgcaggccgg ctcggtgacc agtgcgcgcg cgatcgccgc ccgttgccgt
     4861 tcgccacccg acagttcgga cggcgtgtgc tgcaggcggt gtccaaggcc gacctcaccc
     4921 agcaccaccg cggcgcgcgc ttccgcctcg gctttcggca gccgccggat gagcagcggc
     4981 atggcgacgt tctcgagcgc gctgaattcg gacagcagat ggtgaaactg atacacgaaa
     5041 ccgaggtggc ggttgcgcag aatcccgcac tgcgcgtcgt cgatcgtggc cagatcgtgt
     5101 cccatcaact ggacactgcc gccgctcgcg gcatcgagtc cgccgagcag gtgcagcagc
     5161 gtgctcttgc ccgaacccga cgcgccgacg atcgccaccc gttcggcgac ctggatttcg
     5221 agatcgaccc ccagcaggac cgggacgtcg gtgtcgccct ggcggaaaat cttgctcagg
     5281 ccctggcagg ccagtaccgg ctcactcata gcgcaaggcc gccgccggat tgacccgcgc
     5341 cgcccgccag ctcggataga gcgtcgccac cagcgccagc acgaaggcaa cgccggtgat
     5401 cgtcgtcacg tctccccact gcaactcgga aggcaggttg gaaatgtagt acacctcctt
     5461 ggccaggaac tgcgtgccga gcaggcgctc gatgaacggc acgacgacat cgacgttgag
     5521 cgccagggcc acgccaccgg caatgccgag gcccagcccg atgaagccga tcagcgcccc
     5581 ctgcacgatg aacaccgcca tgatgctcaa cgggctggca ccgagcgtgc gcagaatggc
     5641 aatgtcggcc tgcttgtcgg ttaccgccat gaccagcgtg gaaacgatgt tgaacgccgc
     5701 tacggcgatg atcagcgaca ggatgatgaa catcatgttc ttctcgatct gtacggcacg
     5761 aaagaaattg gcatggctgc gcgtccagtc gctgatgaag gcattggtgt cgagggagcc
     5821 caccagcaga cgcgccacgc gcggcgcctg gaagagatcg tcgagtttca ggcgtacgcc
     5881 ggacactcga tcatccatgc ggtacaggcg ctgcgcgtcg gcaagatgga tcagcgccag
     5941 gccactgtcg tattcgtaca tgccgacctc gaacagcccg acgacggtaa aggttttcag
     6001 acgcggtacc acgccggccg gggtcaccac gccttgcggc gcgatcagcg tgacgcggtc
     6061 gccaacgaag acgcccagtg cgcgcgccag ttcggatccc aggatgatat tgaagcgatc
     6121 cggaagcagg gcgtcgaact tgccgctctt catgtgccgg cgaaaatcgg ccacctggtc
     6181 ttccagatcc ggctggatgc cgcgcaccat ggcgccgcgc acggcttgcc cgtaggccag
     6241 catgccctgc gcctgcacga agggcgcggc ggcgatgacc tgaggttgta ccgcggcctg
     6301 cgctgccacc cgctgccagt cgaccagttc gccgccgacg ccactgatct cgacgtgcga
     6361 gacgacagcg aggatccgcg accgcagctc ggtctgaaaa ccgttcatca ccgacagcac
     6421 gacgatcagc gcggcaacgc ccagggcgat gccgaacatc gaaatcagcg agatgaagga
     6481 gatgaagtgg ttgtgcttct tggcccgcgt ataacgcagg ccgatcagca actcgtaggg
     6541 aagggccatc ggcggcggca actcatatca gggcagcgct gcgaacgccg caaaggcaaa
     6601 aagcgctatg ctacactttt tccgggagcg accgcccctt gccggctccc gtcttcccgc
     6661 caggcagcgg cagcgcgcgt ccaccccctg ccgccaccgc cgaatacagc ccatcccgat
     6721 gcagctcacc ctggttgttc ccgaactcat ctggcccgaa cccgacgacc gcgaaagcct
     6781 cgacgccctc ccctgcccgg gcctgaacac gctgatcgcg cgcagccggt gca
//
LOCUS       DAOSIT010000047         6817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10127_length_6817_cov_9.217391, whole genome shotgun sequence.
ACCESSION   DAOSIT010000047 DAOSIT010000000
VERSION     DAOSIT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10127_length_6817_cov_9.217391"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLL14_02120"
     CDS             complement(<1..922)
                     /locus_tag="PLL14_02120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG02744.1"
                     /translation="MYAERVLSGMRPTGSLHLGHYHGVLKNWVRLQHEYPCLFFVADW
                     HALTTQYDDPRGIVNAAWDMVIDWLAAGVDPVRATLFIQSQVPEHAELHLLLSMMTPL
                     GWLERVPTYKDQQEKLENKDLSTYGFLGYPLLQSADILIYRADKVPVGEDQVPHIEFS
                     REIARRFNHLYGREPGFEDKAREAVRKLGAKRARRYEELRTRFQQQGEQAAIGWAHAL
                     LDELPGLAHDDRERLCGYLEGTGKMILVEPAALLTEASRMPGLDGQKMSKSYGNTITL
                     REDAASVTHKIRRMPTDPARIRRTDPGEPAN"
     gene            complement(940..1596)
                     /locus_tag="PLL14_02125"
     CDS             complement(940..1596)
                     /locus_tag="PLL14_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG02745.1"
                     /translation="MDPAMIQTIAIAALPVIFAITLHEAAHGYAARHFGDPTAWQAGR
                     ITANPLRHIDLVGTIIVPAIILLLSDNSILFGWAKPVPVNFGRLRHPKSDMLWVAAAG
                     PGANLAMLLFWAALAKMAWLLPINFFSLPMARMAEIGMSINIALMVLNLFPLPPLDGG
                     RIAVSLLPRDAAWQFAKLERFGFPILLLLLFTGILGSLLMPVMRLVGGLVEIIFQLPS
                     "
     gene            complement(1607..2233)
                     /locus_tag="PLL14_02130"
     CDS             complement(1607..2233)
                     /locus_tag="PLL14_02130"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG02746.1"
                     /translation="MIQYVTIHPDNPQERLLQKAVAVVRHGGIIALPTDSSYALGCHL
                     GDKAAVERIRSLRGVDDRHHLTLMCRDLSQIGQFARVDNARYRLLKATTPGSYTFILE
                     GTRELPRRVLHPKRKTIGLRIPAHTVTLALLEALGEPLLTSTLILAGDEDRLTEGWEI
                     RDRLDGQIELILDGGHCGIEPTTVVDLTGALPVLIRAGLGALAPLGLD"
     gene            complement(2256..3125)
                     /locus_tag="PLL14_02135"
     CDS             complement(2256..3125)
                     /locus_tag="PLL14_02135"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HOG02747.1"
                     /translation="MHSYFRCPYWSVSVFNCDLHCHSTCSDGLLPAAEVARRAAANGV
                     EMLALTDHDDLDGLPAARAVADAVGMAFVNGVEISIEWESLQIHILGLAFNRADPALN
                     AGLAAIRSGRIERARRMSAELEKVGIAGAFEGAMRFAANPSLVSRAHFGRYLVESGVC
                     KDLRSVFESYLVPGRPGYVDHRWATLSDSLRWILGAGGLAVVAHPGRYKLPRAEMRRF
                     LGEFKDLGGQAIEVMSGSHTAEHVEAFGRLAREHDFLASRGSDFHGPGESYVDLGKLA
                     PLPAGLTPVWQAF"
     gene            3370..4347
                     /gene="cysK"
                     /locus_tag="PLL14_02140"
     CDS             3370..4347
                     /gene="cysK"
                     /locus_tag="PLL14_02140"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG02748.1"
                     /translation="MSRWYEDNSLSIGRTPLIRLNRIVEGTSATLFAKIEGRNPAYSV
                     KCRIGAAMIWDAEKRGLLGPGKELVEPTSGNTGIALAFVAAARGIPLTLTMPETMSVE
                     RRKLLVAYGARLVLTEGAKGMSGAIAKAEEIAASDPNRYVLLQQFKNPANPEIHERTT
                     GPEIWDDTDGRIDILVSGVGTGGTITGVSRYIKNTRGKAITTVAVEPSASPVLTQTRA
                     GEPLKPGPHKIQGIGAGFVPQVLDLSLVDVIEQVSNEEAVDYARRLAREEGILSGISC
                     GAAVAAAVRHARKPENAGKTIVVVLPDSGERYLSSVLFEGIFDAAGLAI"
     gene            complement(4384..>5001)
                     /locus_tag="PLL14_02145"
     CDS             complement(4384..>5001)
                     /locus_tag="PLL14_02145"
                     /inference="COORDINATES: protein motif:HMM:NF037012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOG02749.1"
                     /translation="MGFSDLRVINDDWVQPGKGFDTHPHQDMEIFTYVLAGALEHRDS
                     MGNGSIIRPGDVQMMSAGTGIRHSEFNPSPQDSVHLLQIWIVPDRLRVAPRYQQVHFP
                     DSEKRGRLRLIISPDGTDGSLSVYQDARVYAGLFDGDERTTLDLGTSRHAFVHLARGS
                     LQINGQRLQAGDGARLRDETAIEITAGDAAEVLIFDLRANGAARS"
     assembly_gap    5002..5101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5102..6156)
                     /locus_tag="PLL14_02150"
     CDS             complement(<5102..6156)
                     /locus_tag="PLL14_02150"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG02750.1"
                     /translation="MSESAQRRLAGRRNLIKFTGASLLLTVSPLASAATNRNSGVLAV
                     RIWPAADYTRVAIEYAAPLKYTHFTVKNPERLVVDLEGIELNNVLESLANKVASDDPN
                     IRLLRAGRYKPGVVRLVMELKGEVNPQVFVLPPVGGYGHRLVLDVYPLNPPDPLLSLL
                     DSKEPRPAAVESRAGSRPDSRPEPPAEAKTEPKPEAPPQVAEQKVVKRQGRPMVDRLV
                     TITLDPGHGGEDPGAVGSGGSYEKNVTLAVAKRLRAKIEAEPNMRAVLTRDSDFFVPL
                     HARVQKARRIQSDLFVSIHADAFVRPDANGSSVFVLSESGASSSAARYLAQKENAADL
                     IGGVNLDVKDPILARTLL"
     gene            complement(6111..6614)
                     /gene="tsaE"
                     /locus_tag="PLL14_02155"
     CDS             complement(6111..6614)
                     /gene="tsaE"
                     /locus_tag="PLL14_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG02751.1"
                     /translation="MHSPDDNHSTFSLHLPDETATASFGRRLAPLLRPGMVVWLDGDL
                     GAGKTTLVRGMLRACNHVGPVRSPTYTLVEIYVISRIYWYHFDFYRFNFPEEFLDAGL
                     AEYFRDDAVCLVEWPEKAAEHAPPPDLVVRFEFAEPGRTLDVVARSEEGRTCLKALNG
                     GWQDAAT"
BASE COUNT         1356 a         2125 c         2060 g         1176 t
ORIGIN      
        1 agttggccgg ctcaccgggg tcggtacggc ggatgcgtgc cggatcggtg ggcatgcgcc
       61 gaattttgtg cgttaccgaa gcggcgtcct cgcgcaacgt gatcgtgttg ccgtaggact
      121 tcgacatctt ctgaccatcg agaccgggca tgcgcgaggc ttcggtcagc agcgcggcag
      181 gctcgaccag aatcattttc ccggtgcctt cgagataacc gcacagccgc tcgcgatcgt
      241 cgtgcgcaag ccccggcaac tcgtcaagca aggcatgtgc ccacccgatc gccgcctgct
      301 cgccctgctg ttgaaaacgt gtgcgcagct cctcgtaacg gcgggcccgc ttcgcaccca
      361 gcttcctgac cgcttccctg gccttgtctt cgaaaccggg ctcgcggccg tagagatggt
      421 tgaagcgacg cgcaatttcg cgtgaaaact cgatatgcgg cacctgatcc tcacctaccg
      481 gtaccttgtc ggcgcgatag atcagaatgt ccgcggactg cagcagcggg taaccgagga
      541 aaccgtaggt cgacagatcc ttgttctcca gtttctcctg ctggtccttg taggtcggaa
      601 cgcgctccag ccagcccagg ggagtcatca tcgacagcag cagatgcaac tcggcatgct
      661 cgggtacctg cgactggatg aacagcgtcg cccggaccgg gtcgacaccc gccgccagcc
      721 agtcgatgac catgtcccac gcggcattga cgatgcctcg cgggtcgtcg tactgggtgg
      781 tcagggcgtg ccagtcggcg acgaagaaca ggcaggggta ctcatgctgc agccgaaccc
      841 agttcttcag caccccgtgg tagtggccca gatgcaggct gccggtcggg cgcatcccgg
      901 agagaactcg ttctgcatac atgttctgtc ttcgtcagtt caggaaggta attgaaaaat
      961 gatctcgacg agtccgccga ccagacgcat caccggcatc agcaacgatc cgagaatacc
     1021 ggtaaataac agcagcagca ggatcggaaa accgaagcgt tcgagtttgg caaactgcca
     1081 ggccgcatct cgcggcaaca ggctgacggc gatgcgccca ccgtcgagcg gcggcaatgg
     1141 gaagaggttg agcaccatca gcgcgatgtt gatcgacatc ccgatttcgg ccatacgcgc
     1201 catcggcaaa ctgaagaaat tgattggcag caaccaggcc atcttggcca gcgccgccca
     1261 gaacagcagc atcgcgaggt tggcgccggg accggccgcc gcgacccaca acatgtcgct
     1321 cttcgggtgc cgcaggcgcc cgaaattgac cggtacgggc ttcgcccaac cgaacagaat
     1381 actgttgtcg gagagcagca ggatgatcgc cggcacgatg atcgtcccga ccagatcgat
     1441 atgcctgagc ggattggcgg tgatccgtcc ggcctgccac gccgtcggat caccgaagtg
     1501 gcgtgccgcg tagccatgcg cggcttcgtg caaggtgatc gcgaagatca ccggcaaagc
     1561 ggcgatggcg atggtctgaa tcatggccgg gtccatacgc ggttccttag tccaggccca
     1621 gcggcgccag cgcgccgagc ccggcgcgaa tcagcaccgg cagtgccccc gtcaggtcga
     1681 cgaccgtcgt cggctcgatg ccgcaatggc caccgtcgag gatcaattca atctggccgt
     1741 cgagtcgatc acgtatctcc cagccttcag tcagccgatc ttcgtcaccg gcgaggatca
     1801 gcgtcgaggt cagcaacggc tcgccaaggg cctcgagcag cgccagcgtg accgtgtgcg
     1861 ccggaatgcg caagccgatg gtcttgcgtt tcgggtgcag gacacgccgt ggcaattccc
     1921 gggtgccttc gaggatgaag gtgtagctgc ctggcgtggt cgccttcaac aagcggtagc
     1981 gggcattgtc gacgcgtgcg aactggccga tctgcgacag gtcgcggcac atcagcgtca
     2041 ggtgatgccg atcatcgacg ccacgcaggc tgcgaatacg ctcgactgca gccttgtcgc
     2101 cgagatggca acccaatgca tagctggagt cggtcggcaa ggcaatgatg ccgccatggc
     2161 gaacgaccgc gacggccttt tgcagcagcc tctcctgtgg gttgtcggga tgaatggtga
     2221 catactgtat catcgcggcg cactccctcc gaggctcaga atgcctgcca gacgggcgtc
     2281 aaacccgccg gcaggggcgc cagtttgccc agatcgacat aactttcacc cgggccatga
     2341 aaatcggaac cgcgcgacgc cagaaagtca tgctccctgg ccaggcgacc gaaagcctca
     2401 acatgttccg ccgtgtggct gcccgacatg acttcgatcg cctgcccgcc gagatccttg
     2461 aactcgccga gaaaacgccg catttcggcg cgcggcagtt tgtagcgccc gggatgagca
     2521 accaccgcca gcccaccagc gcccaggatc cagcgcagcg aatcggacag cgtcgcccag
     2581 cggtgatcga cgtagcccgg ccgacccgga accagataag actcgaagac gctgcgcaga
     2641 tccttgcaaa cgccgctttc caccagataa cgaccgaaat gcgcacgact gaccaggctc
     2701 gggttggcgg cgaagcgcat cgccccctcg aaggcaccag cgatgccgac cttttccagt
     2761 tcggccgaca tccgtcgcgc acgctcgata cgccccgaac ggatcgccgc caggccggcg
     2821 ttcaacgccg gatcagcgcg gttgaaggca agccccagaa tgtggatctg cagcgactcc
     2881 cattcgatcg agatttccac gccattgaca aacgccatgc ccacggcgtc cgcaaccgca
     2941 cgcgcggccg gcaggccgtc aaggtcatcg tggtcggtca acgccaacat ctccacaccg
     3001 ttcgcggccg cgcgacgagc cacttcggca gcgggcaaga gaccgtccga gcaggtggaa
     3061 tggcagtgga gatcgcagtt gaacacggat acggaccagt atgggcagcg gaagtaactg
     3121 tgcatgataa cgaatatggt tagatcgtgc gagcgaaagc cttgtccaac ggggtatgag
     3181 cgcgtcaatt gcgtccgcta acgctatgac tacccccgcg caaaaagcat aaccatagaa
     3241 aaacttcgtc attggcttga agccgacgcc gggttagcat tattcgctgc gaactgaacg
     3301 ctcggcgctg gatgcggctg ggccgccaac cagttttcca gacaccattg cccaccccag
     3361 gagatccgca tgtccagatg gtacgaagac aactcccttt cgatcggccg cacgccgctg
     3421 attcggctca accgcattgt cgagggcaca tcggcaaccc tgtttgccaa gatcgagggg
     3481 cgcaacccgg cttattcggt caagtgccgg atcggcgccg caatgatctg ggacgcggaa
     3541 aagcgtggct tgctggggcc gggcaaggaa ctggtcgaac ccaccagcgg caataccggt
     3601 atcgcgctcg cctttgtcgc tgctgcacgc ggcatccccc tgaccctgac catgccggag
     3661 acgatgagcg ttgaacggcg caaactactg gtggcctatg gcgccagact ggtattgacc
     3721 gaaggtgcca agggaatgag cggcgccatc gccaaggccg aggaaatcgc cgcctccgac
     3781 cccaaccgct acgtgttgct gcagcaattc aagaatcccg ccaacccgga aattcacgag
     3841 cgcacgaccg gtcccgagat ctgggacgac accgacgggc ggatagacat cctcgtttcc
     3901 ggcgtcggca ccggcggcac catcaccggc gtttctcgtt acatcaagaa tactcgcggc
     3961 aaggcaatca ctacggtcgc tgtcgagccc tccgccagcc cggtgctcac tcagacgcgt
     4021 gcgggcgaac cgctcaaacc gggcccgcac aagatccagg gcatcggcgc cggcttcgtg
     4081 ccgcaagtcc tcgacctctc tctggtggat gtcatcgaac aggtgtcgaa cgaagaggcc
     4141 gttgactacg cccgtcgcct ggcacgggaa gaaggcattc tctccggcat ctcctgcggg
     4201 gcagccgtgg ccgcggcagt acgtcacgcc cgcaagcccg agaatgcagg caagaccatc
     4261 gtcgtcgtcc tgcctgactc cggcgagcgc tatctcagtt cggtgttgtt cgagggcatc
     4321 ttcgacgccg ccggcctggc gatctgacaa aacggccgag cgggtgggga atctggggga
     4381 aggctagctg cgcgcggctc cgttggctcg aaggtcgaag atcaggactt cggcggcatc
     4441 tccggcggtg atctcgatcg ccgtttcgtc gcgtaggcgc gcgccgtcgc cagcctgcag
     4501 ccgctggcca ttgatctgca gcgagccgcg cgcgaggtgc acgaaggcgt ggcgcgaagt
     4561 accgagatcc aaagtcgtgc gttcgtcacc atcgaagagg ccggcgtaca cacgtgcgtc
     4621 ctgatacacc gacagcgagc cgtcggtacc atcgggtgaa atgatcagac gcagccgacc
     4681 gcgtttctcg ctatccggga aatgcacctg ctggtagcgc ggcgcgaccc gcaaacggtc
     4741 gggaacgatc cagatctgca acagatgcac ggaatcctga ggcgacggat tgaactcgct
     4801 gtggcgtatg ccggtaccgg cactcatcat ctgcacgtca cccggacgaa tgatcgatcc
     4861 gttgcccatc gaatcgcggt gttcgagggc tcctgcgagg acataagtga aaatctccat
     4921 gtcctggtgt ggatgggtgt caaaaccctt gcccggctga acccagtcgt cgttgatcac
     4981 ccgcaggtcg gagaaaccca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nagcagcgta cgcgccagga tggggtcctt gacatcgagg ttcaccccgc cgatcagatc
     5161 ggcggcgttc tccttttgtg ccagatagcg tgcggcggaa ctcgacgccc cgctctcgga
     5221 gagcacaaag accgaagatc cgttggcgtc gggtctgaca aatgcatcgg catggatcga
     5281 gacgaaaaga tccgactgga tgcgtcgggc cttctgcacc cttgcgtgca ggggtacgaa
     5341 aaaatcggaa tcgcgcgtca ggaccgcgcg catgttgggt tcggcctcga tcttggcgcg
     5401 taatcttttg gcaaccgcca gggtgacgtt tttctcgtag ctgccaccac tgccgacagc
     5461 gcccgggtcc tcgccgccgt gccccggatc gagggtgatg gtcaccaggc gatcgaccat
     5521 tggccgaccc tggcgtttga ccactttctg ttcagcgacc tgcggcgggg cttcgggttt
     5581 cggctctgtc ttggcttcgg cgggcggttc cgggcgactg tcgggcctgc tgccggctcg
     5641 gctctcgacg gccgcggggc gcggctcctt gctgtccagc agcgacaaca acggatcggg
     5701 cggattcagg ggatagacat caagaaccag tcggtgcccg taaccaccca ccggcggcag
     5761 gacaaacacc tgcggattga cttcaccctt cagttccatg accaggcgta cgacgcccgg
     5821 cttgtaacgt cccgcacgca acagcctgat gttcgggtcg tccgaagcta ccttgttggc
     5881 cagactctca agcacattgt tcaattcaat gccctcgagg tcgaccacca ggcgctccgg
     5941 gttcttcacc gtgaagtgcg tatatttcag gggcgcagcg tattcgatgg ccacccgcgt
     6001 gtagtcggct gccggccaga tacgaacggc gagaacaccg gagttgcgat tggtcgccgc
     6061 cgacgcgagc ggactgaccg tcaggagcag cgaggcaccg gtaaacttga tcaggttgcg
     6121 gcgtcctgcc agccgccgtt gagcgctttc agacatgttc tcccctcctc gctgcgcgcg
     6181 acgacatcca gcgtccgccc cggttcggca aattcgaagc gaacgacgag atctggtggc
     6241 ggcgcgtgct ccgcagcctt ctcgggccac tcgacaaggc acaccgcatc atcacggaag
     6301 tattccgcaa gccccgcatc caaaaactct tctggaaagt tgaaacgata gaaatcaaag
     6361 tgataccagt atatcctcga aatcacataa atttcaacca gtgtgtaggt agggcttctt
     6421 accggtccga catgattgca ggcacgcaac atcccgcgca ccagcgtggt cttgccggcc
     6481 cccagatcac cgtcgagcca gacgaccatg ccgggacgca gcaggggcgc cagtcgccgg
     6541 ccaaaactgg cagtcgccgt ctcgtccggc aggtgcaggc tgaacgtgga gtggttatca
     6601 tccgggctat gcacgcaggt gactccataa caggcgctga acccaagccg gggcaaggcc
     6661 cgaccgatta tgcgcaagtg gtcagcagga tcaaggcttg gggacaggaa ctcggctttg
     6721 cggcgatcgg cattgcgact gccgacgtta cctcagccag gccaaggctg atgcgctggc
     6781 tggaactggg gcggcacggc gaaatggatt atatggc
//
LOCUS       DAOSIT010000048         6816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10131_length_6816_cov_10.666913, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000048 DAOSIT010000000
VERSION     DAOSIT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10131_length_6816_cov_10.666913"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLL14_02160"
     CDS             complement(<1..332)
                     /locus_tag="PLL14_02160"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOG02752.1"
                     /translation="MPTFQEVILRLQEFWDQQGCALLQPYDIEVGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPSPENIQELYLDSLRALGI
                     DTSQHDIR"
     gene            complement(426..1949)
                     /gene="lnt"
                     /locus_tag="PLL14_02165"
     CDS             complement(426..1949)
                     /gene="lnt"
                     /locus_tag="PLL14_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOG02753.1"
                     /translation="MGWHCLGASLLGAAGVVAFAPVGWFPANWLSLGGLFGLLCAVTG
                     SQGQVRHGALIAAAYGLGQFLTGVSWVYVSLSVFGGMPAALAGLATLLFCGVLAIFPA
                     LAGALFVRLAAPGYWPRALLFAALWVLTEGLRAWAFTGFPWLSAGYSQTPPSPLAGYV
                     PLLGVHGASLASALLGTLVFEIVRRWLSTTDCPTHGWLRWCPALPLLAAAAILATGGL
                     LREVRWTQEEGEPLSVALLQGNVAQEMKWRPERFADSLHSYYRLALENPARLTVLPET
                     ALPAFLDQIPADYLDALQQLALRQQGELLLGIPVAEGRHYYNAAMSLGASGRQRYDKV
                     HLVPFGEFVPPGFAWFMAMANIPMSDFTAGSPTQAPLRLAGQRVGVNICYEDAFGSEI
                     IGALPTATLLVNLSNVAWFGDSLAPAQHLQIAQMRALETGRMLLRATNTGMTAIVDVD
                     GRVVSALPAFTRGALVGEVRGHSGATPFVRWGNWPVIGLALLSIAVSGRRRTGSGTA"
     gene            complement(1954..2796)
                     /locus_tag="PLL14_02170"
     CDS             complement(1954..2796)
                     /locus_tag="PLL14_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOG02754.1"
                     /translation="MDSNPAHKPGFFERLSSLLVHAPEDREQLMQLLHAAHQRQLLNA
                     DALGIIEGALAASEMSVREVMVPRPMMEVVDVQDSLPEVIAQVNTTLHSRFPVINGSR
                     DNVIGILLTKDLLRVPHTDGFNLRDWVRPAVFIPEYKRLDVLLREFRVSRNHMAIVID
                     EYAGIAGLITIEDVLEQIVGDIADEYDFDEADDNIQLDQNGAYRVKAHTTVADFNVAF
                     DTRFSDEKCNTVGGLILNHLGRVPQFNETIAIDDVNFQVLRADSRRIYTLVVSRSPAA
                     DESE"
     gene            complement(2887..3351)
                     /gene="ybeY"
                     /locus_tag="PLL14_02175"
     CDS             complement(2887..3351)
                     /gene="ybeY"
                     /locus_tag="PLL14_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG02755.1"
                     /translation="MPKSKRLDLGVQYACKRATLPGRKQVRTWVRRTLKATGRSGGQI
                     SVRFVDVEEGRRLNSGYRDKDYATNILSFPYQIEPSVCGDLVLCAPVLVREANEQGKS
                     LAAHCAHLIVHGLLHLQGFDHETGDEQAAAMEGHERRILAALGYADPYRDEL"
     gene            complement(3338..4369)
                     /locus_tag="PLL14_02180"
     CDS             complement(3338..4369)
                     /locus_tag="PLL14_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG02756.1"
                     /translation="MTARTVTAKRPSTELLLSPVNNKQLANLCGVLDENLRQIETALD
                     VSIARRGERFTLRGETAQTARASEALQQFYAQAKDTLSVDEIQLGLIELLNRPVRNIS
                     SGGGVSPALMTRKSELHGRTPRQIEYLKSIQEHDITFGTGPAGTGKTYLAVASAVDAF
                     ERELVQRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLMGFERVGKLFER
                     GSIEIAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGIGAKAVVTGDVTQIDL
                     QRGQKSGLIEARQILKEVRGIAFTEFLKEDVVRHPLVARIVSAYEAHTAALEAREAAA
                     NAARKHAKE"
     gene            complement(4366..5733)
                     /gene="miaB"
                     /locus_tag="PLL14_02185"
     CDS             complement(4366..5733)
                     /gene="miaB"
                     /locus_tag="PLL14_02185"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG02757.1"
                     /translation="MAKKLFIRTFGCQMNEYDSDKMADVLAASEEIVKTDTPDDADII
                     LFNTCSVREKAQERVFHDLGRVRLLKLANPDLVIGVGGCVASQEGAAIVARAPYVDVV
                     FGPQTLHRLPQLIAERRRLGKSQVDISFPEIEKFDHLPPARVEGIAAFVSIMEGCSKF
                     CSFCIVPYTRGEEVSRPFDDVLTEVADLAAQGVKEVTLLGQNVNAYRGAMSGSGQAPA
                     GEEAADLALLIEYIAEIPGIERIRYTTSHPREVSPRLIEVYARVPKLVSHLHLPVQSG
                     SDRVLAAMKRGYTTLEYKSLVRKLRAARPDLSLSSDFIVGFPGETAEDFEKTMKLIDE
                     VGFDASFSFLYSARPGTPAAELADDTPQPVKLERLLRLQKRIDELAQAVSAAMVGSVQ
                     RVLVEGLSKKDRNELAGRTDNNRVVNFAGHATDNTALLDRFLEVRITSAMPHSLRGEI
                     VSRPA"
     gene            complement(5750..6205)
                     /locus_tag="PLL14_02190"
     CDS             complement(5750..6205)
                     /locus_tag="PLL14_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02758.1"
                     /translation="MIVLQGSNTHRSKNRHLAHDGLPSGCRLVYNAAMDIPSALLYSI
                     LSAIAEAALAPAPDPQMAYEGQALSRSLPDEARQGVMQPPTGDGFVTISGRELPLAPT
                     AQIRSRQNLIVMPMQIQNPVEVVYITDASGAIYRVWMLTPSEASVSRPR"
     gene            6245..>6816
                     /locus_tag="PLL14_02195"
     CDS             6245..>6816
                     /locus_tag="PLL14_02195"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HOG02759.1"
                     /translation="MLESYRAHVAERAALGIPPLPLTAKQTEELIGLLKNPPAGEEAN
                     LVELITYRVPAGVDDAAKVKAEFLARVAKGEESCALISKEKATELLGTMLGGYNVKPL
                     IDLLAGPTGTIAAEALKKTLLVFDFFHDVKELADKDNANARAVMQSWADAEWFTSRPP
                     VPASQKLTVFKVTGETNTDDLSPAPDAWSRP"
BASE COUNT         1213 a         2121 c         2260 g         1222 t
ORIGIN      
        1 aagcggatgt cgtgctggct ggtatcgatc ccgagtgcgc gcagcgagtc gagatagagt
       61 tcctggatgt tctccggcga cggcttcagc accacctgat actggtagta atgctgcagg
      121 cggttcgggt tctcgccgta gcggccgtcc tttggccgac gcgacggctg tacgtaggcc
      181 gcgttccaag gttcgggtcc gatcgcgcgc aggaaggtcg cggtatgaaa ggtaccggca
      241 ccgacttcaa tgtcgtacgg ctgcagcagc gcacagccct gttggtccca gaactcctgg
      301 agacgcagaa tgacttcctg aaacgtaggc atgggagagc ttcgtgcaga accaaaagcc
      361 gggattttac tttgtttctc ggccgacaag aacctgcttc ctccggctcc cggcaagtcg
      421 gcatgtcagg ccgttcccga tcccgttctg cggcgacccg acacggcaat cgacaacaag
      481 gcgagaccga tgaccggcca gtttccccag cgtacgaaag gtgtggctcc cgaatggccg
      541 cgtacttcgc cgaccagtgc gccgcgcgtg aaagcgggca gggccgagac cacgcgaccg
      601 tcgacgtcga cgattgcggt catgccggtg ttggtggccc gcagcagcat gcgcccggtt
      661 tcgagggcgc gcatctgggc aatctgcagg tgttgtgccg gcgccagcga gtctccgaac
      721 caggcgacgt tggacaggtt gaccagcagc gtcgcagttg gcaaagcgcc gataatctcg
      781 ctgccaaatg cgtcttcgta gcagatgttc acccccacgc gttggcctgc aaggcgcagc
      841 ggcgcctgcg tcggtgagcc ggcggtgaag tcggacatcg ggatgttggc catcgccatg
      901 aaccaggcaa aacccggcgg aacgaactca ccgaaaggca ccagatgtac cttgtcgtag
      961 cgctgccggc ctgacgcccc cagactcatc gccgcattgt agtagtgcct gccttcggct
     1021 accgggatgc cgagcagcag ttcgccttgc tggcgcagcg cgagttgctg cagggcgtcg
     1081 agatagtccg cgggaatctg gtcgaggaaa gcgggcaggg cggtttcggg cagaaccgtg
     1141 agccgtgccg ggttctccag tgccaaccgg tagtagctgt gcagggaatc ggcgaaacgc
     1201 tcgggccgcc atttcatctc ctgcgccaca ttgccttgca gcaaggccac cgacagcggc
     1261 tcgccttctt cctgcgtcca gcggacttcg cgcagcagtc cgccggtcgc caggatcgcg
     1321 gcagctgcaa gcagcggcag ggctggacac cagcgcagcc agccgtgtgt cgggcagtcg
     1381 gtggtagaca gccaacgcct gacgatctcg aagaccaacg tgccgagcag cgccgaagcc
     1441 agcgacgcgc cgtggacgcc gagcagtggc acatagccgg caagcgggct cggtggtgtt
     1501 tgcgagtagc cggcggacag ccacggaaaa ccggtgaagg cccaggctcg caagccctcg
     1561 gtcagcaccc acagggcggc gaagagcagg gcgcgaggcc agtagccggg cgcggccagg
     1621 cgaacgaaga gcgcgccggc caaagccggg aaaatcgcca gcacgccgca gaacagcaac
     1681 gtcgccaggc ccgccagcgc tgccggcatg ccgccaaaga cgctgaggct gacatagacc
     1741 caggatacgc cggtcaggaa ctggccaaga ccataggcgg cggcgatcag cgcaccgtgg
     1801 cggacctgtc cctggctgcc ggttaccgcg cagagcaggc cgaagaggcc gccgaggctc
     1861 agccagttgg cgggaaacca gccgacgggc gcaaaagcga cgacacccgc agcaccgagc
     1921 aggctggcgc cgaggcagtg ccagcccagg cccctattca gattcgtcag ccgcaggcga
     1981 gcgcgatacg accagggtat agatgcgccg gctgtcggcg cgcaacacct ggaaattcac
     2041 gtcgtcaatg gcgatcgttt cgttgaactg tggtactcgc ccaagatggt tcaggatcag
     2101 gccaccgacg gtattgcatt tttcgtcgct gaagcgggtg tcgaaagcaa cattgaagtc
     2161 cgccacggtg gtgtgcgcct tgacccgata cgccccgttc tgatcgagct gaatattgtc
     2221 gtcagcctcg tcgaaatcgt actcgtccgc gatatcgccg acgatctgtt cgagcacgtc
     2281 ttcgatggtg atcagaccgg caatgccggc gtattcgtca atgacgatcg ccatgtgatt
     2341 gcgcgagacg cggaactcgc gcagcaggac gtcgaggcgc ttgtattcag ggatgaacac
     2401 cgccggtcgt acccagtcgc gcaggttgaa accgtcggtg tgcggcaccc gcagcaggtc
     2461 cttggtcaac aggatgccga tgacgttgtc acggctgccg ttgatcaccg gaaagcgcga
     2521 gtgcagggtc gtgttgacct gggcgatcac ttctggcagc gaatcctgga catcgacgac
     2581 ctccatcatc ggccgcggca ccatgacttc ccggacgctc atttccgaag ccgccagagc
     2641 gccttcgatg atgcccaagg catcggcatt gagcaactgg cgctggtgcg ccgcgtgcag
     2701 gagttgcatg agttgttcgc ggtcctccgg tgcatgcacc agcagcgagg agagacgttc
     2761 aaaaaatccg ggtttgtgag cggggttgct gtccatagga gccttttggc gtgcgggtcg
     2821 cgtcggcggc cgacgtatcg gtccactccg ggatgccgga gaagacctgt ggtggtgagg
     2881 tgagggtcac aactcgtcgc ggtaggggtc cgcgtagccc agggcggcga gtatgcgccg
     2941 ttcatggcct tccattgcgg ccgcctgttc gtcgcccgtt tcatggtcga agccctgcag
     3001 gtgcagcagg ccatggacga tcagatgcgc gcaatgcgct gccagcgact tgccctgttc
     3061 gttggcctcg cggacgagca ccggcgcgca caacaccagg tcgccgcaga cgctgggttc
     3121 gatctggtag gggaaggaca ggatgttggt cgcgtagtcc ttgtcgcggt acccgctgtt
     3181 gagtcgccgc ccttcctcga catcgacaaa gcgcacgctg atctgccccc ccgatctgcc
     3241 ggtcgctttc agcgtgcgac gtacccaggt ccggacctgc ttcctgccgg gtagggtggc
     3301 tcttttgcag gcgtactgca cgccgagatc caggcgctta ctctttggca tgctttcttg
     3361 ccgcgtttgc cgcggcttcg cgagcctcga gcgccgcggt atgcgcttcg taagcggaga
     3421 cgatgcgtgc caccagggga tggcggacca cgtcttcctt gaggaattcg gtgaaagcga
     3481 tgccgcgaac ctccttgagg atctggcggg cttcaatcag gccgctcttc tggccgcgtt
     3541 gcaggtcgat ctgggtgaca tcgccggtga ccaccgcctt ggcgccgatg ccgatgcgcg
     3601 tcaggaacat cttcatctgc tcgggcgtcg tgttctgcgc ttcgtcgagg atgatgaagg
     3661 cgtggttgag ggtgcggccg cgcatgtagg ccagcggggc aatctcgatc gagccacgct
     3721 cgaagagctt gccgacgcgc tcgaagccca tcagatcgta cagcgcatcg tacagcggtc
     3781 gcaggtacgg atcgaccttc tgcgccaggt cccccggcag gaagccgagg cgctcgccgg
     3841 cctcgacggc gggacgggtc agcacgatgc gctgcaccag ctcgcgttcg aaggcgtcga
     3901 ccgcggaagc caccgccagg taagtcttgc cggtgccggc gggaccggtg ccaaaggtga
     3961 tgtcgtgttc ctggatgctt ttcaggtatt cgatctgccg cggcgtgcgc ccgtgcaact
     4021 cgctcttgcg ggtcatcagc gccggcgaga cgccgccgcc cgagctgatg ttgcgcaccg
     4081 gccggttgag cagttcgatc aggccgagct ggatttcgtc gaccgacaag gtatccttgg
     4141 cctgcgcgta gaactgctgc agcgcctcgg aggcgcgcgc ggtctgcgcg gtctcgccgc
     4201 gcagcgtaaa gcgctcgccg cggcgggcga tcgagacgtc gagtgccgtc tcgatctggc
     4261 gcaggttctc gtcgagcacg ccgcagaggt tggcgagctg cttgttgttg accggcgaca
     4321 gcagcagttc ggtgctcggc cgcttggcgg tgaccgtcct ggcggtcatg ccggtcggct
     4381 cacgatttca ccgcgcagcg agtgcggcat cgccgaggtg atgcgcacct ccaggaagcg
     4441 atcgaggagc gccgtgttgt cggtggcatg gccggcaaaa ttgacgacgc ggttgttgtc
     4501 ggtgcgcccg gccagttcat tgcggtcctt cttcgacaga ccttcgacga gcacgcgctg
     4561 gacgctgccg accattgccg cggaaacggc ctgcgccagt tcgtcgatgc gcttctgcag
     4621 acgtaacagg cgttcgagct tgaccggctg cggcgtatcg tcggcgagtt ccgccgccgg
     4681 ggtgcccgga cgcgcgctgt agaggaaaga gaacgaggca tcgaagccga cctcgtcgat
     4741 cagtttcatg gttttctcga aatcctcggc cgtctccccc ggaaagccga cgatgaaatc
     4801 cgaggatagc gacaggtcgg gacgtgccgc ccgcagcttg cgcaccaggc tcttgtattc
     4861 aagcgtcgta tagccgcgtt tcatcgccgc gaggacgcga tccgatcccg actgaaccgg
     4921 cagatgcagg tgcgatacca gcttcggcac cctggcatag acctcgatca gccgcggcga
     4981 gacctcgcgc gggtgcgaag tcgtgtagcg gatgcgttcg atgccgggaa tctcggcgat
     5041 gtactcgatc agcagcgcga ggtcggccgc ctcctcgccg gcaggggcct ggccgctgcc
     5101 gctcatcgcg ccccggtagg cattgacgtt ctggccgagc agcgtgacct ccttgacccc
     5161 ctgcgccgcc aggtccgcca cttcggtcag gacgtcgtcg aagggacgcg aaacctcttc
     5221 accgcgggtg tagggcacga tgcagaacga acagaacttg ctgcagccct ccatgatcga
     5281 aacgaaggcc gcaataccct cgacccgggc gggtggcaga tggtcgaact tctcgatctc
     5341 ggggaaggaa atgtcgacct gcgacttgcc cagccggcgc cgctcggcga tcaattgcgg
     5401 caggcgatgc agggtctgcg ggccaaagac gacgtctaca tacggtgcgc gtgcgacgat
     5461 cgccgcccct tcctgactcg ccacgcagcc gccgacgccg atgaccaggt cgggattggc
     5521 aagcttcagc aggcgcaccc ggccgagatc gtggaacacg cgttcctgcg ctttctcgcg
     5581 taccgagcag gtgttgaaga ggatgatgtc cgcgtcgtcg ggagtgtcgg tcttgacgat
     5641 ttcttcggag gcagcgagca cgtcggccat cttgtccgaa tcgtactcgt tcatctgaca
     5701 cccgaaggtg cggatgaaca gttttttggc catcttttac ctgattgtct tagcgtgggc
     5761 gcgacaccga ggcttcgctc ggcgtcagca tccagacgcg gtaaatcgcg ccggaagcgt
     5821 cggtgatgta aacgacctcg acagggttct ggatctgcat cggcatcacg atcaggttct
     5881 gccggctgcg gatctgcgct gtcggggcca ggggcaactc gcgtccgctg atcgtcacga
     5941 agccgtcacc cgtcggtggc tgcatgacgc cctgcctggc ctcgtcgggt agcgagcggc
     6001 tcagtgcctg tccttcgtat gccatctgcg ggtcgggcgc cggcgccagg gcagcctcgg
     6061 cgatggcgct gaggatgctg tacagcaggg cggaagggat gtccatcgcc gcattataga
     6121 caaggcggca ccccgatggc agtccatcgt gcgccaggtg ccggttcttc gaccggtggg
     6181 tgttgctccc ttgcagtaca atcatgcttt tgctgccgta caacctttga gaaaggacgt
     6241 cgccgtgctg gaatcctatc gtgcccatgt tgccgagcgc gctgcgcttg gtattccgcc
     6301 gctgccgctg accgcgaagc agaccgagga actgatcgga ctgctgaaga acccgcccgc
     6361 gggtgaagag gcaaatctcg tcgagctgat cacctaccgt gtgccggccg gagtggacga
     6421 cgcagccaag gtcaaggccg agttcctggc ccgagtggcc aagggcgaag agtcctgcgc
     6481 cctgatctct aaggaaaagg cgaccgaact gctgggaacg atgctcggcg gttacaacgt
     6541 caagccgctg atcgacctgc tcgccggccc gaccggtacg atcgccgccg aagcgctcaa
     6601 gaagaccctg ctggtgttcg atttcttcca cgatgtcaag gaactggccg acaaggacaa
     6661 cgccaacgcc agggcggtca tgcagagctg ggcggatgcg gagtggttca cctcgcgtcc
     6721 gccggtgccc gcctcgcaga agctgaccgt tttcaaggtc accggcgaaa ccaacaccga
     6781 cgacctgtcg ccggcgcccg acgcctggtc gcgccc
//
LOCUS       DAOSIT010000049         6769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10213_length_6769_cov_10.061066, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000049 DAOSIT010000000
VERSION     DAOSIT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_6769_cov_10.061066"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLL14_02200"
     CDS             <1..699
                     /locus_tag="PLL14_02200"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG02760.1"
                     /translation="RLARGLLLEINDLGLPCATEFLDTITPQYTADLIAWGAIGARTT
                     ESQVHRELASGLSCPVGFKNGTDGNIRIAIDAIRAAQTPHHFLSVTKAGHSAIVSTGG
                     NEDCHVILRGGKAPNFDAAHVDAACKEIAAAGLAARLMIDASHANSNKRFKQQIEVAR
                     DTASQLAAGDERIIGVMIESHLVEGRQDLVPGQPLAYGMSITDACLGWEDSLLVLETL
                     ANGVRARRLVEAAQ"
     gene            complement(807..962)
                     /gene="rpmG"
                     /locus_tag="PLL14_02205"
     CDS             complement(807..962)
                     /gene="rpmG"
                     /locus_tag="PLL14_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOG02761.1"
                     /translation="MRDKIKLESTAGTGHFYTTTKNKKTMPGKMEIKKYDPKARKHVI
                     YKEIKLR"
     gene            complement(977..1210)
                     /gene="rpmB"
                     /locus_tag="PLL14_02210"
     CDS             complement(977..1210)
                     /gene="rpmB"
                     /locus_tag="PLL14_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG02762.1"
                     /translation="MARVCQVTGKGPMAGNNVSHANNRTKRRFLPNLQYRRFWIESEN
                     RWVRLRVSNAGLRTIDKNGIDVVLAELRARGEI"
     gene            1358..2920
                     /locus_tag="PLL14_02215"
     CDS             1358..2920
                     /locus_tag="PLL14_02215"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOG02763.1"
                     /translation="MSIRGVIAASEPHTAEAGARLLRRGGNAIDAIVAAKLAASVTEL
                     PLTSLGGGGACVWGDAADGYEVLDFFAHAPGQGLAGPPVLDFAPVTVDFGQTTQVFHV
                     GKAAAAVPGELVGLLELHRRAGRLPLREVVAPAAAWARDGFAISPQIAMIVGLIAPIA
                     HRSPAVARLFLPGNVLPQAGDRLANPDLGDFLHALGEGDPDAQVARYWTSLIDDFGPA
                     HGGLITAQDVAAYAPVVRQPLRVPFGSYTVLTNPPPSAGGGLIGVGLRLAETLGLGEE
                     AFLSEQHQLAIAAVLATVSQIRQSGYDQRLHRDPASIRDLVAGVALPAWVEVARALRG
                     ENPLGATTHISVIDAQGMAAAMTTSNGEGCGHVLPGLGIHINNFLGEDDINPAGFHQL
                     PPGMSMSTMMSPTLVLAGDRACFALGSGGSNRIRSAILQALLNLLAYRRPLDEAVNAA
                     RLHVEGRKLWFESPGLAAGAADALHSAWPEATRFDVKSMFFGGVNCVADYDGRLCGAG
                     DRRRGGVVVVAE"
     gene            complement(2962..>3156)
                     /locus_tag="PLL14_02220"
     CDS             complement(2962..>3156)
                     /locus_tag="PLL14_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG02764.1"
                     /translation="ADVSNLIGAGNPNAAATPHILPEIARNPGIFPPSEDLQRLEMLR
                     DFDPKTRRVLSRMWTEIKVR"
     assembly_gap    3157..3256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3257..3804)
                     /locus_tag="PLL14_02225"
     CDS             complement(<3257..3804)
                     /locus_tag="PLL14_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG02765.1"
                     /translation="MKALRVLLAGLLLAVLATLVHAEDVLYLFIWNTYLSEDTVRRFE
                     TQCRCRLNQDFYSDNEEMLAKLEAGAIGYDLIVPTGNAVETLLRKKALRPLDKTKLPN
                     LKNLKTEFRDPWYDPGLRYAVPYATSVTLLGYNATRLAERGLPTDSWALIFEPQHLEK
                     LKGKVTVLNSQRELMAAAMKYLG"
     gene            complement(3801..>4576)
                     /locus_tag="PLL14_02230"
     CDS             complement(3801..>4576)
                     /locus_tag="PLL14_02230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02766.1"
                     /translation="FGLWTAALAVYAFLYLPLAVVVIFSFNDSLLNAEWVGFTTRWYG
                     QLFEDTEMLRAAANSLFIALGTATLATVLGTMAGIALQRWRSGFLRALPFLVLAPVAM
                     PEILLGVSLLLFFRQVLDLTLGLVSILIAHITFSIGFVAIIVRVRLAGLDASIFEAAR
                     DLGAGPWQTFRWVTLPLILPGILAGFLMSFTLSIDDFVITFFVAGVGVTTLPLQIYSM
                     IKVAVSPEVNAVSTLLMALTLTLIALASKLAPDTLRSRA"
     assembly_gap    4577..4676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4677..5203)
                     /locus_tag="PLL14_02235"
     CDS             complement(<4677..5203)
                     /locus_tag="PLL14_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02767.1"
                     /translation="MTESRQTGHCPDPGEALRKWLVSLPPTLFLLVFFVAPALLMVVA
                     SFQQVGDFGGLLPVLENGQSTLSLEAYRFFFGDWIYAEIFLRSFLVAAATTLLCLLLA
                     YPLALLIATGGKRHRDLLVLLVILPLASNFLIRIYAWMIVLGPVELLYSPFAVIVGMV
                     YVHLPFMILPLYTNL"
     gene            complement(5196..6281)
                     /locus_tag="PLL14_02240"
     CDS             complement(5196..6281)
                     /locus_tag="PLL14_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02768.1"
                     /translation="MALLEIRKVSRRFGNLKALDDVSLSIEAAEFFTLLGPSGCGKTT
                     LLRLIAGFDEPDSGEVLLDAQPLAGIPAEKRPVHTVFQSYALFPHMTVAQNVAFPLQM
                     AGKHTQEIGRRLGEVLEMVHLADKAQQFPNELSGGQKQRVALARALINKPRLLLLDEP
                     LAALDAKLREQITSELIALQRDVGITFVFVTHSQQEALAVSHRVAVMNGGRVEQVGAP
                     EQIYGYPRNRFVADFIGTINLLPVEVRVAGGDALRLVANGLGEIVSTPVAEASAGQRG
                     AYAVRPERVRIFSAGDVCELDNHFQGTVNEHLYLGDVTVYKIALDNGVVLEALLANSA
                     SGRTGFLERGAVVGVGWHRSAGVYLRD"
     gene            6517..>6769
                     /locus_tag="PLL14_02245"
     CDS             6517..>6769
                     /locus_tag="PLL14_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOG02769.1"
                     /translation="MKAMLLDTQGQPLRLAEVPRPHAGANQILLAVQACGVCRTDLHV
                     MDGDLHGPRLPLILGHEIVGRVVEKGEQVERFAIGQRVGV"
BASE COUNT         1242 a         2124 c         2112 g         1091 t
ORIGIN      
        1 cgcctggcgc gcggcctgct cctcgaaatc aacgatctgg ggctaccctg cgccaccgag
       61 tttctcgata ccatcacccc acaatacacc gccgacctga ttgcctgggg ggcgatcggc
      121 gcacggacca cggaatcaca ggtccatcgc gagctggcct cgggcctgtc gtgtccggtc
      181 ggcttcaaga acgggaccga cggcaacatc cgcattgcca ttgatgcgat ccgtgcggca
      241 cagacaccgc accattttct ctcggtcacc aaggcgggcc actcggcgat cgtatctacc
      301 ggcggcaacg aggactgtca cgtgatcctg cgcggcggca aggctcccaa cttcgacgcg
      361 gcgcacgtcg acgccgcctg caaggaaatc gcggcggccg gcctcgccgc acgcctgatg
      421 atcgatgcgt cgcacgccaa cagcaacaag cggttcaagc aacagatcga agtcgccaga
      481 gacacggcca gccagctcgc ggccggtgac gagcggatca tcggcgtgat gatcgagtcg
      541 catctggttg aaggacgtca ggatctggta ccggggcagc cgctggccta cggcatgagc
      601 atcaccgacg cctgtctggg ctgggaggat tcgctgctgg ttctcgaaac cctggccaat
      661 ggggtacgcg cgcgccgcct ggttgaagcg gcgcagtgac cggcccgtga cgccgttggc
      721 gtcgagcgct ggctgagccg acggcgccag ccttcgatcg ttggcggcgg ctgccggtcg
      781 ccggggcgcg ctcctgagcg cgccgcctag cggagcttga tttccttgta gatcacgtgc
      841 ttgcgggctt tcggatcgta cttcttgatc tccatcttgc ccggcatggt cttcttgttc
      901 ttggtggtgg tgtagaagtg gccggtaccg gcggtagact ctagcttgat cttgtcacgc
      961 atggctgctc tcctggtcag atctcgccgc gcgcgcgcaa ctcggcgagc acgacgtcga
     1021 taccattctt gtcaatcgtt cgcaaaccag cgttcgatac acgcaagcgc acccagcggt
     1081 tctcgctctc gatccagaag cggcgatact gcagattcgg aaggaagcgg cgcttcgttc
     1141 tattgttggc atgggaaaca ttgttccccg ccatcgggcc cttgcccgta acctggcaaa
     1201 cacgcgccat gattgttgct ccaattgttt ttgggaagaa agcccgcgat tttagcccaa
     1261 gcagcgggtc aggttcaagc aatttcccaa ccgttggcac tattcggcag gcggacgaca
     1321 ccttcccgac cgccgcgcca tcgcgtattc tctgcccatg agcattcgtg gagtgatcgc
     1381 ggcttccgag ccgcatacgg ccgaggccgg agcgcgcctc ctgcgccgcg gcggcaacgc
     1441 gatcgacgcc atcgtcgccg ccaaactcgc ggcaagcgtc accgaactgc cgctgacctc
     1501 gctcggcggc ggcggcgcct gcgtctgggg cgacgccgcc gacggttacg aagtcctcga
     1561 tttttttgcc catgcgcccg gacagggcct cgccggtcca ccggtcctcg acttcgcacc
     1621 ggtgacggtc gatttcggac aaaccacgca agtctttcac gtcggcaagg cggcagcggc
     1681 ggtgcctggc gaactcgttg gcctgctcga actgcaccgg cgtgccgggc gcctgccgct
     1741 gcgtgaagtc gtcgcgccgg cggccgcctg ggcacgcgac gggttcgcca tcagcccgca
     1801 gattgcgatg atcgtcggcc tgatcgcacc gatcgcccac cgttcgccgg cggtggcccg
     1861 cctgttcctg cccggcaacg tgttgccgca agccggcgat cgcctggcca atcccgacct
     1921 cggggacttt ctgcacgcgc tcggcgaagg cgatcccgac gcgcaggtgg ccaggtactg
     1981 gacttcgctg atcgacgact tcggaccggc acacggcggg ctgatcaccg cgcaggacgt
     2041 cgccgcctac gcgccagtgg tacggcagcc cctgcgggtc cccttcggct cctatacggt
     2101 gttgaccaac ccaccgccct cggcgggcgg cgggctgatc ggcgtcggcc tgcgcctcgc
     2161 cgagacactc ggtctgggcg aggaggcatt cctgtcggag cagcatcaac tggcgattgc
     2221 ggcggtgctg gcaacggtgt cgcagatccg ccagtcgggt tacgaccagc gcctgcaccg
     2281 cgacccggcg tcgatccgtg acctggtcgc cggcgtcgcc ctgcccgcgt gggtcgaggt
     2341 cgcgcgcgcg ctgcgcggcg aaaacccgct cggcgcgacc acgcacatct cggtgatcga
     2401 tgcgcagggc atggcggcgg cgatgaccac cagcaatggc gaaggttgcg gccatgtgct
     2461 gccgggactg ggaatccaca tcaacaactt tctcggcgag gacgacatca accccgccgg
     2521 cttccatcag ttgccgccgg gaatgtcgat gagcacgatg atgtccccga cgctcgttct
     2581 cgccggcgat cgagcgtgct tcgcgctcgg cagcggcggt tcgaaccgga ttcgcagcgc
     2641 gatcctgcag gccctgctca acctgctggc ctatcgacgc ccgctcgatg aagccgtgaa
     2701 cgccgcgcgc ctgcacgtcg aaggccggaa actctggttc gagagccctg gcctggcagc
     2761 cggagccgcc gacgcgctgc actccgcgtg gccggaggcg acccgcttcg acgtgaaaag
     2821 catgttcttc ggcggcgtca attgcgtcgc cgactacgat ggccgattgt gtggcgccgg
     2881 cgaccgcaga cgtggcgggg tggtggtggt cgccgagtag cgccggtttg ggttgtgggc
     2941 gagcggcacc acctcacgcg ctcagcgaac cttgatctcc gtccacatac gcgagagcac
     3001 gcggcgggtc ttggggtcga aatcgcgcag catttcgagt cgctgcaggt cctccgatgg
     3061 tggaaagatg ccggggttgc gggcaatctc gggaagaatg tgcggtgttg ccgcggcgtt
     3121 cggattgccg gcgccgatca ggttcgagac atcggcnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnccga ggtatttcat cgccgcggcc atcagttcac gctggctgtt
     3301 gagtaccgtc accttccctt tcagcttttc gaggtgctgc ggctcgaaga tcagcgccca
     3361 gctgtcggtc ggcaaaccgc gttccgccag ccgtgtcgcg ttatagccga gcagcgtcac
     3421 cgaggtcgca taaggcacgg cgtaacgcag gcccggatcg taccagggat cgcgaaactc
     3481 ggtcttgagg ttcttcaggt tcggcagttt tgtcttgtcg agcggacgca gcgctttctt
     3541 ccgcagcaag gtctcgaccg cgttgccggt gggaacgatc aggtcatagc cgatggcccc
     3601 ggcttcgagc ttggccagca tctcctcgtt gtcggaatag aaatcctgat tcagtcggca
     3661 gcgacattgc gtctcgaaac ggcggacagt gtcttccgaa aggtaggtat tccagatgaa
     3721 gaggtacagg acgtcctcgg cgtgcaccag cgtcgcgagc acggccagca gcaagccggc
     3781 aagaaggacg cgcagcgcct tcatgccctg ctccgcaggg tgtccggcgc cagtttcgag
     3841 gccagcgcga tgagcgtcaa ggtcagcgcc atcagcagcg tcgacacggc attcacttcg
     3901 ggactcaccg ccaccttgat catcgaataa atctgcaacg gcagcgtcgt gacgccaacg
     3961 ccggcgacga aaaaggtgat cacgaaatcg tcgatggaaa gggtaaagct catcaggaag
     4021 ccggccagga tgcccggcag gatcagcggc agtgtcaccc agcgaaaggt ctgccaggga
     4081 ccggcgccga gatcgcgggc cgcttcgaag atgcttgcat ccaggcccgc gaggcgaaca
     4141 cggacgatga tcgcgacaaa accgatcgag aaggtgatgt gcgcgatcag gatcgacacc
     4201 agaccaagcg tcagatcaag cacctggcgg aagaacagca gcagcgaaac tccaagcaga
     4261 atctcgggca tcgccaccgg cgccaggacc agaaagggca aggcacgcag gaagcccgac
     4321 cgccagcgct gcagcgcaat accggccatg gtgccgagca ccgtcgccag cgtcgccgtg
     4381 ccgagagcga tgaacagcga attggccgcc gcgcgcagca tctccgtgtc ttcgaagagt
     4441 tgaccgtacc agcgcgtggt aaagccaacc cattcggcat tgagcagcga gtcgttgaac
     4501 gagaagatca cgacgaccgc cagcggcaaa tagagaaagg cgtaaaccgc cagagccgcg
     4561 gtccagaggc cgaacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcga
     4681 gattggtata caacggcagg atcatgaacg gcaggtgaac atagaccatg ccgacgatca
     4741 ccgcaaacgg cgagtacagc agttcgaccg ggccgagcac gatcatccag gcgtagatgc
     4801 gaatcaggaa gttgctggcc agcgggagga tcaccagcag caccagcaga tcgcgatgcc
     4861 gcttgccacc ggtcgcgatc agcagggcca gcggataggc cagcagaagg cagagcagcg
     4921 tcgtcgccgc ggcgacgaga aaggagcgca gaaagatttc cgcgtaaatc cagtcgccga
     4981 agaaaaagcg gtaggcctcc agcgacagtg tcgactggcc gttctccagg acaggcagca
     5041 gcccgccaaa atcccccacc tgctgaaacg aggcgacgac catcagcagc gccggtgcaa
     5101 caaagaagac cagcagaaac agcgtcggcg gcaggctgac cagccacttg cgcagagctt
     5161 ctcccgggtc ggggcaatgg ccagtttgcc ggctttcagt cacgcagata aaccccggcg
     5221 ctacggtgcc agcccactcc gaccaccgca ccacgttcaa ggaaaccagt gcgtccggag
     5281 gccgagttgg cgagcagcgc ctcaagcacg acgccgttgt cgagcgcaat cttgtagacc
     5341 gtgacatcgc ccagatacag gtgctcgttc acggtgccct ggaaatggtt gtccagctcg
     5401 caaacgtcac cggcgctgaa aatgcgcacg cgttccggtc gcaccgcgta ggcgccgcgt
     5461 tgaccggcgc tggcctcggc taccggtgtg ctgacgatct ccccgaggcc gtttgccacc
     5521 aatcgcaacg catcgccacc ggccacccgg acttcgaccg gcagcaggtt gatggtgccg
     5581 atgaaatcgg cgacaaagcg attgcgcggg tagccgtaga tctgctcggg agcgccgacc
     5641 tgctcgacgc ggccaccatt catcaccgcg acgcgatgcg agaccgccaa cgcttcctgc
     5701 tgcgaatggg tgacaaagac gaaggtgatg ccgacgtcac gctgcagggc gatcagttcg
     5761 ctggtgatct gctcgcgcag cttggcgtca agcgcggcca gcggttcgtc gagcaacagc
     5821 agacgcggct tgttgatcag cgcgcgggca agtgccacgc gctgcttctg gccaccggaa
     5881 agttcgtttg gaaactgctg tgccttgtcc gcgagatgca ccatttccag gacttcgccc
     5941 agacggcggc caatctcctg agtgtgtttt ccggccatct gcaggggaaa ggcgacattc
     6001 tgcgcgacgg tcatgtgcgg aaacagcgcg taactctgga aaaccgtgtg caccggccgc
     6061 ttctcggcag gaatgccggc caggggctgc gcatcgagca gcacctcgcc gctgtcgggc
     6121 tcgtcgaagc ccgcgatcag gcgcagcagc gtggtcttgc cgcagccaga ggggcccagc
     6181 agggtaaaaa actcggcggc ctcgatcgac agggaaacgt cgtcaagggc cttgaggttg
     6241 ccgaaacgtc ggctgacttt acggatctcg agtagcgcca tggcgacgca gagtctatac
     6301 cagaaaatcc gtggctgtga agggggcatg ccggtgcgcg cccaggagcg aatccgcccg
     6361 gaagcaacgg cgccagcttt accgccagcg ttggcgggtt tgcgtgcagc gaacacccgc
     6421 gcttagaatg ggtcgcccct cgtggtgaca cgcccagtgc ccgacccccc cgcgccaccg
     6481 cgagcgcagc cagcagcaac ccgacaggag aaacccatga aagccatgct tctcgacact
     6541 cagggccagc ccttgcggct ggccgaagtc ccccggccgc acgccggggc gaaccagatc
     6601 ctgcttgcgg tgcaggcctg cggtgtctgc cgcaccgatc tgcacgtcat ggacggcgat
     6661 ctgcatggac ccaggttgcc gctcatcctc ggccacgaaa tcgtcggcag ggtcgtcgag
     6721 aaaggcgagc aggtggaacg tttcgcaatc ggtcagcgtg tcggcgtgc
//
LOCUS       DAOSIT010000050         6699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10367_length_6699_cov_7.678356, whole genome shotgun sequence.
ACCESSION   DAOSIT010000050 DAOSIT010000000
VERSION     DAOSIT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10367_length_6699_cov_7.678356"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLL14_02250"
     CDS             <1..404
                     /locus_tag="PLL14_02250"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286056.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOG02770.1"
                     /translation="DILNHNLETVPRLYRQARPGADYAHSLAFMKAFKARYPQVPTKS
                     GLMVGLGETDDEILEVLRDLRANDVEMLTIGQYLAPSGHHLPVDRYVHPEIFRMYEQQ
                     ALAMGFTGAACGPMVRSSYWADVQAHSAGVA"
     gene            complement(427..945)
                     /locus_tag="PLL14_02255"
     CDS             complement(427..945)
                     /locus_tag="PLL14_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02771.1"
                     /translation="MKTHVLPDCLRRLLIRCVAHRYYSLVVTAIAFWSTMTFTFPFVA
                     VLIPAVLLSPRRWRSLGLLCGIASGCGAAALVEIFQYLGSEFVFSRYPDLLQTQAWQT
                     ASEWLQHWGLLALLIIAGSPIPQTPALLFCAFADVSTAGILVAVAIGKTVKYLFLAWA
                     TAHYPGRFIRYN"
     gene            1333..1824
                     /locus_tag="PLL14_02260"
     CDS             1333..1824
                     /locus_tag="PLL14_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOG02772.1"
                     /translation="MKLVFLVLASLLSGFAFGERAPALGALGEWPDTPPIISGKASFY
                     GAAFAGRRTASGDNFHHQTISGASNRFPLGTLVAVRRVLSARCVVVLINDRMGHRSRV
                     IDLSREAAVRLGMIDAGVTEVEVVPLPKSLIYHEHACTLAFQPPVHLHAAEGSGLASQ
                     VKK"
     gene            1989..>3082
                     /gene="prpC"
                     /locus_tag="PLL14_02265"
     CDS             1989..>3082
                     /gene="prpC"
                     /locus_tag="PLL14_02265"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF009006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HOG02773.1"
                     /translation="MDELREGELPRAKKWVALSGVVAGDTAICSVGRAGNDLYYRGYD
                     VAALAEDATFEEVAYLLLYGRLPSPTQLNQYKKKLKTLRGLPVMLRPMLENLPASAHP
                     MDVLRSGCSALGTILPEAQDHNPGAAREIADRLIASFGSMLLYWYHWSHNGRRIETET
                     DDDSIAAHFLHLLRGKAPSALQASCLDKSLVLYAEHEFNASTFAARTITGTGADLYAA
                     ISGAIGALSGRRHGGANEVAFEIQSRYNDADHAELDIRRRLENREIIIGFGHPLYTLS
                     DPRNKVIRAVAQQLCTDGDNLVLFEIASRIEAVMWEQKRMFPNVDWFSGVAYHMLAVP
                     TPMFTPLFVIARTSGWAAHVIEQRIDGKIIR"
     assembly_gap    3083..3182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3183..4532
                     /locus_tag="PLL14_02270"
     CDS             <3183..4532
                     /locus_tag="PLL14_02270"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HOG02774.1"
                     /translation="GAFETARYCLIDALGCGLEALGYPACSKLLGPIVPGTVVPQGAR
                     VPGTDFQLDPVQAAFNIGTLIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTRMA
                     HGQAPLEMRRVLEAMIKAYEIQGVLALENSFNRVGLDHVVLVKVATAAVCTWLMGGGR
                     QEIINAVSQAFVDGQPLRTYRHAPNTGSRKSWAAGDATARGVLLALFSLKGEMGYPTV
                     LSARTWGFYDVLFKGQAFRLSRPYGSYVMENVLFKISFPAEFHAQTAVECAVKLHPQV
                     VGRLDEIARIVIRTHESALRIIDKKGPLHNPADRDHCLQYMVAVGLIEGRLTCEDYED
                     SAAADPRIDALRAQMEVSEEPRFSADYLDPAKRSIANAVQVFFTDGSRTAELVAEYPL
                     GHCRRRAEGIPVLVEKFRTNLARRFSAAQQAAIEQSSRDVQHLGTLAVNEYIDQYVP"
     assembly_gap    4547..4646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4647..5868
                     /gene="pssA"
                     /locus_tag="PLL14_02275"
     CDS             <4647..5868
                     /gene="pssA"
                     /locus_tag="PLL14_02275"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG02775.1"
                     /translation="ARERILISALYLQDDTAGREVLAAIYAASAVRPDLQIAVFVDWH
                     RAQRGLVGKAASPGNAALYVEMARRLGPGVPIYGVPVQSREWMGVMHLKGFVIDDALL
                     YSGASINDVYLQRFDRYRLDRYHLIENRALADSIAGLMTRILQTNTAVRRLDVEPQPK
                     SGSLRGAIVGLRRVLAKSRYATTAGSVGNGEVGITPLLGFGHRDNQLNAVILQLIKRT
                     ERRLVVFTPYFNLPGPIRRALEGRLRRRCRVTIIVGDKTANDFYISPEDPFKTIGALP
                     YLYESNLRRFCKTHQGDIDEGLLDVQLWSHEHHSFHLKGLLSDDDYALLTGSNLNPRA
                     WRLDLENGLLIHDPQGGLHSQHQAELARILARTRRLDHHTALDDTDTYPVPVQRILKR
                     LARTRADRLLNQVL"
     gene            6052..6486
                     /locus_tag="PLL14_02280"
     CDS             6052..6486
                     /locus_tag="PLL14_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02776.1"
                     /translation="MKLTRIASLLVFAAASATAQVSVSVGGPGGVNVNTGGAGGVSVD
                     TGNGGGAKSGKTTTSGKGNAAYVNPGKSSSSITHSPGTTVSTGKGDDDDDASGISVVN
                     GNVKIDGVKVPATATRWTGKDGTKYRIKRSSKGNVSVEEIDD"
BASE COUNT         1260 a         1921 c         2033 g         1285 t
ORIGIN      
        1 cggacattct caatcacaac ctggagacgg tgccacgcct ttaccggcag gcacgccccg
       61 gcgccgacta cgctcattcg ctggcattca tgaaggcatt caaggcacgt tacccgcagg
      121 ttcccaccaa gtctgggctg atggtcggtc tcggtgaaac cgatgacgag atcctcgaag
      181 tgctgcgtga cctgcgtgca aatgatgtcg aaatgctcac cattggtcag tacctcgcac
      241 cgtccggcca ccatctgccg gtggaccgtt acgttcaccc ggagatcttc aggatgtacg
      301 agcaacaggc tctggccatg ggtttcactg gcgcggcctg tgggccgatg gtgcgctcga
      361 gctactgggc ggatgtgcag gcgcatagtg ccggcgttgc ctgatcggtg gtttggccaa
      421 ccatcctcag ttgtagcgga tgaagcgtcc cggatagtgg gccgtggccc aggcgagaaa
      481 aagatacttg acggtcttgc cgatggccac cgcgaccagg atgcccgctg tagacacgtc
      541 ggcgaaagcg caaaaaagga gtgccggggt ttgcggtatc ggcgagccgg cgatgatcag
      601 cagggccagc aggccccagt gttgcagcca ttcgctggcg gtctgccagg cctgagtctg
      661 cagcagatcg gggtagcgcg agaaaacgaa ctccgagccg aggtactgga agatttcgac
      721 cagtgcggcg gcaccgcagc cactggctat gccgcacagc agaccgagcg agcgccaacg
      781 tctgggagag agcagaaccg ccgggatcag gacggcgacg aaggggaagg tgaaggtcat
      841 cgtcgaccag aaggcgatcg ccgtcacgac gagcgagtaa tagcggtggg cgacacagcg
      901 tatcagcagg cgtcgcaagc agtcgggaag aacatgagtt ttcattgtcg agacaacttg
      961 agcggtcacc tttggtttgt cgtgtggcga tggctgcgct caatggtgtg gcagcttgat
     1021 cgccgcgcag cttgtgtgtc ggacagcacg caggatatgc ttacggcgtc gactcttgac
     1081 ccttgccacc cgtctccgcg gagttttcct gccatgcgcc agttcgcgca aattacccgg
     1141 attgtaagcg tactcctcct cagtgtgcag ttcctggcgc tggtgcgtag cgatgtgccg
     1201 aacaatgcgg tcgcttcctg atgtctgtcc agcaggtcga agcgatttgc cgggagggca
     1261 tgcggaaact tcccggcaag cgtaacgtgc aatacctctc tctcttgtgg ccgccctgag
     1321 gagccatacc ctgtgaaact tgtttttctg gtgctggctt cattactctc gggtttcgcc
     1381 ttcggcgaac gcgctcctgc tctcggggcc ttgggagaat ggcccgacac gccgccaatc
     1441 atttccggca aagccagctt ttacggtgcc gcgtttgccg gacgaaggac agcctctggc
     1501 gacaactttc atcatcagac gatctcgggg gccagcaatc gctttcccct gggcaccctg
     1561 gtcgcggttc gccgggtgtt gtctgcccgg tgcgtggtgg tcttgatcaa tgaccggatg
     1621 gggcatcgca gtcgggtcat cgacctgtcg cgtgaggcgg ctgtacgctt gggaatgatc
     1681 gacgcgggcg tgaccgaggt ggaagtcgtc ccgctgccga aatcgctgat ctatcacgaa
     1741 catgcatgca cgctggcgtt ccagcctccg gtacacctgc acgctgcaga gggttcgggg
     1801 ttggccagtc aggtgaagaa gtgaagttcc cggtgccaag aatgctgacg ttcgatggcg
     1861 cctgttcaaa gcgtcgcctg cgccaaccgg ggaaatcgag tggcaggcaa gcatcgccgc
     1921 cggggagagc cggcgcgtat gttgtgcgtg tcgcatgttg agtggctgca cgaaaggggg
     1981 agacgaagat ggacgagttg cgggaaggag aactacccag ggccaagaaa tgggttgcgc
     2041 tgtccggtgt ggttgccggc gatacggcga tctgttcggt cgggcgtgcg ggcaacgatc
     2101 tgtactaccg tggttacgat gtggcggcac tggccgagga tgccaccttc gaggaggtgg
     2161 cgtacctgct gctctacggg cgcttgccca gtccgacgca gctcaatcag tacaagaaaa
     2221 aactcaagac cttgcgtggt ttaccggtga tgctcagacc gatgctcgag aacctgcccg
     2281 cctccgctca tcccatggac gtcctgcgca gcggctgctc ggctctcggc acgattcttc
     2341 ccgaagcgca ggaccataac cctggtgcag cgcgcgagat tgcggatcgg ctgatcgcca
     2401 gcttcgggtc gatgttgctc tactggtatc actggtcgca caacggccgg cgcatcgaaa
     2461 ccgagaccga tgacgactcg atcgccgcac atttcctgca tctcctgcgg ggcaaggcac
     2521 cttcggcact gcaggccagc tgtctcgaca agtcgctggt tctctatgcc gagcatgagt
     2581 tcaatgcctc gacttttgcc gcacgcacga tcactgggac gggcgctgat ctctatgcgg
     2641 cgatcagcgg cgccatcggt gcgctgagtg gtcgccgaca cggcggagcc aacgaggtgg
     2701 ctttcgagat ccagagccga tacaacgacg ccgaccacgc cgaactggac attcgccgcc
     2761 gcctcgaaaa cagggagatc atcatcggtt tcgggcatcc gctgtacacc ctctccgacc
     2821 cgcgtaacaa ggtgattcgt gctgtggctc aacaactgtg caccgatggc gataacctgg
     2881 tgctgttcga gatcgccagt cgcatcgagg ccgtgatgtg ggagcagaaa cgcatgtttc
     2941 ccaacgtcga ttggttctcg ggcgtcgcct atcacatgct ggccgtgccg acaccgatgt
     3001 ttaccccatt gtttgtgatt gcgcgtacca gcggttgggc agcgcacgtc atcgagcagc
     3061 gcatcgatgg aaagatcatc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnggggcctt cgagaccgcg cgctactgtc tgatcgacgc cctgggttgc gggctcgaag
     3241 cgctcggata tccggcatgc agcaagctgc tcgggccgat cgttcccggc acggtggtgc
     3301 ctcagggcgc aagagtgccg ggtaccgatt ttcaactcga cccggtgcag gccgctttca
     3361 acatcggcac gctgattcgc tggctggatt tcaacgatac ctggctggcg gccgaatggg
     3421 gtcatccgtc cgacaacctt ggcggcattc tcgccactgc cgattggttg tcgcgcaccc
     3481 gtatggctca cggtcaggca ccactggaga tgcgccgggt tctcgaagcg atgatcaagg
     3541 cctacgagat ccagggtgtc ctggcgctcg aaaacagttt caatcgcgtc gggctggacc
     3601 atgtcgtgct ggtcaaggtc gccaccgctg ccgtttgcac ctggctgatg ggcggtggtc
     3661 gtcaggagat catcaacgcc gtgtcacagg cgttcgtcga tggacaaccc ttgcgcacct
     3721 atcgacacgc cccgaacacc ggttcgcgga agtcctgggc ggctggcgac gccaccgccc
     3781 ggggcgtgct gctggcgctg ttttcactca agggtgagat gggctatccc acggtgctga
     3841 gcgcacgcac ctggggcttc tacgatgtgc tcttcaaggg gcaggcgttc cgcctgtcgc
     3901 ggccctatgg cagctacgtg atggagaatg tgctgttcaa gatcagtttt ccggctgagt
     3961 tccacgccca gacggccgtc gagtgtgcgg tgaaactgca cccgcaggtc gtcggacggc
     4021 tcgacgagat cgcccgaata gtcattcgca cccacgaatc ggcattgcgg atcatcgaca
     4081 agaagggtcc gttgcacaat cctgccgacc gcgatcactg cctgcaatac atggtcgccg
     4141 tcggcctgat cgaaggtcgg ctgacctgcg aggactacga ggacagcgcc gcggcagacc
     4201 cgcgcatcga tgccttgcgg gcgcagatgg aagtcagcga ggaaccccgt ttctcggccg
     4261 actatctgga cccggccaaa cgctcgatcg ccaacgccgt acaggtattc tttaccgatg
     4321 gcagccggac ggcagaactg gtcgcggaat accctctcgg gcactgtcgt cgacgtgccg
     4381 aggggatccc tgtgctggtg gagaaattca ggaccaatct cgcgcgcagg ttttccgcag
     4441 cacagcaggc agccatcgag cagtcgtcgc gcgatgtgca gcatctggga acgctggcag
     4501 tcaacgaata catcgatcaa tatgtccctt gatgcagaat tgccggnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnncgcc agagagcgga tcctgatcag cgcactttat
     4681 ctccaggatg ataccgccgg cagagaggtg ctggcggcca tctacgccgc cagtgctgtc
     4741 cggccagacc tgcagatcgc ggtcttcgtc gactggcacc gcgcgcagag aggtctcgtc
     4801 ggcaaggctg cgagccctgg caatgccgcg ctctacgtcg aaatggcccg ccgccttggt
     4861 ccaggcgtcc ccatatatgg cgtaccggtg cagagtcggg agtggatggg ggtgatgcac
     4921 ctgaagggct tcgtcatcga cgacgccctt ctctatagcg gtgccagtat caatgacgtc
     4981 tacctgcaac gcttcgatcg ctaccgcctc gatcgctatc acctgatcga gaatcgcgca
     5041 ttggccgaca gcatcgccgg tctgatgacc cgcatcctgc agacgaacac ggctgttcgt
     5101 cgcctggatg tcgagccgca gccgaaatcc ggttcgctac gtggcgcaat cgtcgggttg
     5161 cgtcgggtgc tggcaaagag tcgctacgcg accaccgccg ggtcggtcgg caacggcgag
     5221 gtgggaatca cgccgttgct gggctttggt catcgtgaca accaactcaa tgccgtgatt
     5281 ctgcaactga tcaagcgcac cgagcgcagg ctggtcgtgt tcacgcctta tttcaacctg
     5341 cccggcccca tccgtcgcgc tcttgaggga aggttgagaa ggcgatgtcg agtcacgatc
     5401 atcgtcggtg acaagaccgc caatgatttt tacatttccc ctgaagatcc tttcaagacc
     5461 attggtgccc tcccttacct ctacgaaagc aatctgcgcc gcttctgcaa gactcaccag
     5521 ggcgatatcg acgaaggact gctcgacgta cagttgtgga gccatgagca tcacagtttt
     5581 catctcaagg gcttgttgtc cgacgacgac tatgccctgc tcaccggcag caacctcaat
     5641 cccagggcct ggcggctgga cctcgaaaac gggttgttga tccatgatcc tcagggaggc
     5701 ctgcacagcc agcatcaggc cgaactggcg cgaatcctgg cgcggacgcg ccgtctcgat
     5761 caccacaccg ccctcgatga cacagacacc tatccagtcc cggtccagcg gattctcaag
     5821 cgcctcgcac gcacccgcgc tgatcgcctg ctgaatcagg tcctttgatt cagcggctgc
     5881 ctttccgcat tcggaacgta cccgtggcaa gaacaccggg tgagaggatc gacacgaaac
     5941 tttcgattga cgaccgccgc cgggatacgg gatactcgtg tagaggccgg tagaagcatt
     6001 gaattagcaa tccttttccc gtagccgttg gttgaacaga aaggctttcg aatgaaactg
     6061 acacgtattg ccagcttgct tgtttttgcc gccgcgtctg cgacggctca ggtttcggtt
     6121 tcggtcggag gacccggcgg cgtcaacgtg aataccggcg gtgccggcgg ggtgagcgtg
     6181 gacaccggta acggaggtgg cgccaagtcc ggcaagacca cgaccagtgg caagggcaac
     6241 gcagcgtatg tgaatcccgg caagagttcg tcctcgatca cccattcacc gggaaccacg
     6301 gtaagcaccg gcaagggtga cgacgacgat gacgcgagtg gcatcagcgt ggtcaatggc
     6361 aatgtgaaga tcgatggcgt gaaggtgcca gcgacggcga cgcgctggac cggcaaggac
     6421 ggcaccaaat accggatcaa gcgctcttcc aaggggaacg tcagcgtcga agaaatagac
     6481 gactgaatgc ctgctttgtt caagtgcagg tcgctctggt gcacttagac ggacgccagc
     6541 acgtccgtcg gatttctgga cgagcgaaca ccctcagaag gtgtggttga cacccaccag
     6601 gaagggctgg ttttgctcgt cccgggcgcc ggtgagtgat gcaaccccat tggggccagc
     6661 ggcgatgctg ttgcggtcgt tatttgtcca gacataggt
//
LOCUS       DAOSIT010000051         6686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10386_length_6686_cov_12.598552, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000051 DAOSIT010000000
VERSION     DAOSIT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10386_length_6686_cov_12.598552"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLL14_02285"
     CDS             complement(<1..563)
                     /locus_tag="PLL14_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOG02777.1"
                     /translation="MQWSDALGKTHGRYSGRPRIVSLVPSVTELLVALGLAEALVGRT
                     GFCIHPRQLLRRVPKLGGTKGFDADKLRALEPTHVIVNIDENRREEVEALSDCVPHLI
                     VTHPLEARDNLDLYRLLGGVFGREATAEALCADFENAWRALRAATEPLPREKVLYLIW
                     REPWLSVARDTYISRMLAAAGWDTLPV"
     gene            600..1325
                     /locus_tag="PLL14_02290"
     CDS             600..1325
                     /locus_tag="PLL14_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG02778.1"
                     /translation="MLAQKLAVVTGAARGIGLAVARQLAAAGCTVLMVGRVAEAIENA
                     AGQLREKGHDVLPHVADVTDASAVAALAARLRREFGRADILINNAGVLLEPKDFALPQ
                     AASVFVVDPDVVAATHACNALAPLRLIQVITPLMRENDWGRIVNVSSSLEQLSEMGGY
                     WPGYRMSKAALNALTRLTAKELEGSPIKVNSVCPGWCRTDMGGKDAARSADEGARGIV
                     WAALLPDDGPSGGFFRDGEAIDW"
     gene            complement(1394..2167)
                     /locus_tag="PLL14_02295"
     CDS             complement(1394..2167)
                     /locus_tag="PLL14_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG02779.1"
                     /translation="MPTPVLKVFITGASSGIGWALSEHYAAQGALVGLCARRAERLRS
                     LSERWPGQVDCYPLDVTDAAALRAAAEDFIARHGVPDIVIANAGVSAGTLSECQEDLA
                     AIRRIMDVNFYGMAATFSPFIGAMRRAAGGRLVGVASLAGIRGLPGAEAYSASKAAAI
                     SYLESLRLELRGSGVRVVTLCPGYIQTPMTESNPFPMPFLIPADVAARRFARVIADGR
                     SYAVVPWQMAIVGKLLRLLPNALYDRLFAGVPRKPRGVS"
     gene            complement(2230..2871)
                     /locus_tag="PLL14_02300"
     CDS             complement(2230..2871)
                     /locus_tag="PLL14_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HOG02780.1"
                     /translation="MHRRISGWFAALTLAALTVVLPARAELVVGRDYVPIVPAQASDN
                     PAKLEVIEFFSYGCPHCSEFHPTVSKWSAALPGDVVFKRVPISFARPQWASLARLYYA
                     LEATGDLARLDAAVFEALHRTGSKLYDDKSIIDWVSAQGVDAKKFSDAYNSFGVVSKA
                     RRGDQMAQAYKIQSVPALAVDGKYLMTGGKGFPELIVLTDQVIAKARGERGKK"
     gene            complement(2891..3712)
                     /locus_tag="PLL14_02305"
     CDS             complement(2891..3712)
                     /locus_tag="PLL14_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG02781.1"
                     /translation="MKPLRTPPARKSGGGTLFGLFIGLVIGVIGVAGVVWYINKAPLP
                     FTTTGQQPKLPPPVASKPPTPAATTPEVPPVATAPVALPGKPGDPIGAEKPRFDFYKI
                     LPGKAEAIPDPKPEEPKPVEAKPTEGKIANNKHTETKPVETRPGETKPAETKAVDSRP
                     VEARPADSKATGAKTAEGKPGEAKQADSGKSKSETLKEPIYLQAGSFQSANEADNQKA
                     RLAMMGAEARIQQVMLQDKVWYRVRLGPYQKLDDVNHLRADLAKQGIDANVVKKD"
     gene            complement(3745..5517)
                     /gene="argS"
                     /locus_tag="PLL14_02310"
     CDS             complement(3745..5517)
                     /gene="argS"
                     /locus_tag="PLL14_02310"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG02782.1"
                     /translation="MSSDLKSHLADLMRAALRSVAPHEADTSIHIERPKQASHGDFSC
                     NLAMQLARSMQRNPRQLAQLLVSELPYSPLVDKTDIAGAGFINFHLRPAAKLEVVRAV
                     LEQGEAFGRSTTGGKRKVLVEFVSANPTGPLHVGHGRGAAYGGSLCTLLAFAGWDVTS
                     EYYVNDAGRQMDILALSTWLRYLELYRRHDESLPFPANAYQGEYVREMARQMLAAHAE
                     RFLRAPAGVIDGVPPLPDPGRTDAAAAQIREAHLDALIANAKRLLGEEWRYVHDFVLT
                     EQLADCRSDLEESGVHFDVWFSEQSLFATGLVTRGVERLQKAGHLYLQDGALWFRSTA
                     FGDEKDRVVQRDNGLYTYFASDIAYHLNKYERGFERMINVWGADHHGYIPRIKGALGA
                     LGLDPEALEVALVQFAVLYRSGWKAQMSTRSGEFVTLRTLRADVGNDACRFFYVLRKS
                     DQHLDFDLDLAKSQSNDNPVYYIQYAHARVCSLLALWGGDLATLAAAPFERLKGAHEL
                     ALAARIGEFPAVVETAAAELAPHLIAFYLKDLAAEFHSYYNAERILVDDSAQKDARLA
                     LAAAVRQVIRNGMTILGVSCPESM"
     gene            5657..>6686
                     /locus_tag="PLL14_02315"
     CDS             5657..>6686
                     /locus_tag="PLL14_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOG02783.1"
                     /translation="MRLFRLAKILSIAHRYGLDEMLFEHEPSGRLAAVSRVLHFRRRF
                     DAPPAVRLRLALESLGPIFVKFGQVLSTRRDLLRPDIADELARLQDRVPPFSPALALT
                     QIERAYGRPASAVFAEFDSTPVASASIAQVHFARLRPEDGGQEVAVKILRPEMHAVIA
                     NDLALLDTLAGLLEKVWSDGKRLKPREVVGEFAKYLYDELDLMREAANCSQLRRNFSG
                     SRLLQVPEVYWDQCTTTVMVMERMRGVPISQTEALLAAGIDLPALSRAGVEIFFTQVF
                     RDGFFHADMHPGNIFIATDADHHGSYIALDFGIVGTLTDTDKNYLAQNFLAFFQRDYK
                     RVATAHIEA"
BASE COUNT         1119 a         2127 c         2251 g         1189 t
ORIGIN      
        1 tcgaccggca gcgtatccca gccggcggcg gccagcatgc gcgagatata ggtgtcgcgg
       61 gcgacgctga gccagggctc gcgccagatc aggtagagca ccttctcgcg cggcagcggt
      121 tcagtcgcgg cacgcagcgc ccgccacgcg ttctcgaagt cggcacacag ggcttcggcc
      181 gtggcctcgc gcccgaaaac gccgcccagc aggcggtaga ggtcgaggtt gtcacgtgct
      241 tccagcgggt gcgtcacgat caggtgcggc acgcagtcgg acaaggcttc gacttcctcg
      301 cggcggtttt cgtcgatgtt gacgatcaca tgcgtcggtt ccagggcgcg cagtttgtcg
      361 gcgtcgaatc ccttggtgcc gccgagcttc ggcacccgcc gcagcaactg ccgcggatgg
      421 atgcagaagc cggtacggcc gaccagcgct tcggcgagtc ccagcgcgac cagcagttcg
      481 gtgacgctgg gcaccagcga aacgatccgt ggccggcccg aatagcggcc gtgtgtcttg
      541 ccgagcgcgt cagaccattg catgatgagg gttcgcggtt caaacaacag gaggaacaga
      601 tgctagctca gaaacttgcg gtcgtcacag gagccgcccg tggcatcggc ctggccgtgg
      661 cacgccagct tgcggccgcc ggctgcacgg tgctgatggt cggacgcgtt gccgaggcga
      721 tcgaaaacgc cgccgggcag ttgcgcgaga agggccacga cgtgctgccg cacgtcgccg
      781 acgtcaccga cgcgagtgcc gtggcggcgc ttgccgcgcg cctgcgccgc gagttcggcc
      841 gcgccgatat cctgatcaat aatgccgggg tgttgctcga accgaaggat ttcgccctcc
      901 cgcaggccgc gagcgtgttc gtcgtcgacc ccgatgtggt cgccgccacg cacgcctgca
      961 acgcgcttgc accgctgcgc ctgattcagg tgatcacgcc actgatgcgc gaaaacgact
     1021 gggggcggat cgtcaatgtt tcgtcgagcc tggagcaact gagcgaaatg ggaggctact
     1081 ggcccggcta ccggatgtcg aaagccgctc tcaacgcgct gacgcggctc accgccaagg
     1141 aactggaagg cagcccgatc aaggtcaact cggtctgccc cggctggtgc cgcaccgaca
     1201 tgggtggcaa ggatgccgcg cgcagcgcag acgagggcgc ccgcggcatc gtctgggccg
     1261 cgctgctgcc cgatgacggt ccgagcggcg gcttctttcg cgacggggaa gcgatcgact
     1321 ggtgacggca ccggcaggcg agcgcgcggc cgagttgcgc agcggcgggc gtccgggaaa
     1381 gtcgctttcc tgatcaagaa acaccgcgcg gcttgcgcgg taccccggca aacagccgat
     1441 cgtagagggc attgggcagc agccgcagca acttgccgac gatagccatc tgccagggca
     1501 caacggcata actcctgccg tcggcgatga cgcgcgcaaa acgccgtgcg gccacgtcgg
     1561 cgggaatcag gaaaggcatc ggaaaggggt tcgactcggt catcggggtc tggatatagc
     1621 ctggacagag ggtgaccacc ctgacgcccg agccgcgcag ttcgagacgc aggctttcga
     1681 gatagctgat cgccgccgcc ttcgaagcgc tgtaggcctc cgcgcctggc aagccgcgga
     1741 ttccggccag cgaggcgact cccaccaggc gaccaccggc ggcgcgacgc atggcgccga
     1801 tgaatggcga gaaagtggcc gccatgccgt agaaattgac gtccatgatc cggcggatcg
     1861 ctgccaggtc ttcctggcac tcgctcagcg tgcctgccga gacgccggcg ttggcaatca
     1921 cgatgtcggg aacgccgtgg cgggcgatga agtcttcggc ggcggcacgc agggccgccg
     1981 cgtcggtcac gtcgagcggg tagcaatcga cctgtcctgg ccagcgttcg gacaggctcc
     2041 gcaagcgctc ggcgcgccgg gcacaaagcc cgaccagcgc tccctgcgcg gcgtagtgct
     2101 cgctcagcgc ccagccgatg ccggaagagg cgccggtgat gaaaaccttg aggacggggg
     2161 tcggcacgcg caggcgtgga acgtcaccgc atcaggcggc agccgactcc caccgggcgg
     2221 gcggcggcgt cacttcttgc cgcgctcgcc gcgtgccttg gcgatcacct ggtcggtgag
     2281 cacgatcaac tcgggaaaac ccttgccgcc ggtcatcagg tacttgccgt cgacagccag
     2341 cgccggaacg ctctgaatct tgtacgcctg cgccatctga tcgccacgcc tggccttgct
     2401 gacgactccg aaggagttgt aggcatcgct gaacttcttg gcatcgacgc cctgtgcgct
     2461 cacccagtcg atgatgctct tgtcgtcgta aagcttgctg cccgtcctgt gcagggcttc
     2521 gaagaccgcg gcatcgagcc gggccagatc gccggtggcc tcaagggcat aataaagcct
     2581 ggccaggctc gcccactgcg gccgcgcgaa ggaaatcggc acgcgcttga agaccacatc
     2641 gcccggcaag gcggctgacc atttgctgac cgtggggtgg aattcgctgc aatgcggaca
     2701 gccgtacgag aagaattcga tcacttcgag tttggccggg ttgtcggacg cctgtgccgg
     2761 gacgatgggc acgtaatcgc ggccgacgac gagttcggcg cgggccggca gaaccactgt
     2821 cagcgcggcc agggtgagcg ccgcgaacca tccactgata cgtcgatgca tgactcttct
     2881 cctgtattgg ttagtctttc ttgaccacat tggcgtcgat gccctgcttg gcgaggtcgg
     2941 cacgcagatg gttgacgtcg tccagtttct gatacggtcc gaggcgaacg cggtaccaca
     3001 ccttgtcctg gagcatgacc tgctggatcc gcgcctcggc gcccatcatc gccagtcgcg
     3061 ccttctggtt gtccgcctcg ttggcgctct ggaacgaacc ggcctgcaga tagatcggct
     3121 ccttgagcgt ttcactcttg ctcttgccgc tgtccgcctg cttcgcctcg ccgggtttgc
     3181 cttcggcggt tttggctccc gtggccttgc tgtccgccgg cctggcttcc accggccggc
     3241 tgtccacggc cttggtttcg gccggtttcg tttcgccagg ccgggtttcg accggcttgg
     3301 tctcggtatg tttgttgttt gcgattttgc cttcggtggg cttggcttcg actggtttcg
     3361 gttcttccgg tttggggtcg ggaatggctt cggccttgcc tggcaggatc ttgtagaaat
     3421 caaagcgcgg cttctcggcg ccgatcgggt cacccggctt gccggggagc gccaccggtg
     3481 ccgtggcgac cggcggcact tcgggtgtgg tggccgccgg ggtcgggggc ttgctggcca
     3541 cgggtggcgg cagcttgggt tgctggccgg tggtggtgaa cggtagcggc gccttgttga
     3601 tataccagac gacgccggcc acgccgatga cgccgatgac caggccgatg aacaggccga
     3661 acaatgtgcc cccccccgac ttccttgcgg ggggggttct aaggggtttc aagtcgcgac
     3721 tcatggaagt cctctgttct ctgattacat cgattcgggg caggagacac ccagaatcgt
     3781 catgccgttg cgaatcacct gcctgaccgc agccgccagt gccaggcgcg catccttctg
     3841 cgccgaatca tcgaccagaa tgcgttcggc attgtaatag ctgtgaaatt cggcggccag
     3901 gtctttgaga tagaaggcga tcaggtgcgg tgccaactcc gcggcggcgg tttcgacgac
     3961 cgcggggaat tcgccgatcc gggccgccag ggccagttca tgcgcgcctt tgaggcgctc
     4021 gaaaggcgcg gcggcgaggg ttgccagatc gcctccccag agtgccagca gcgaacacac
     4081 gcgggcgtgc gcatactgga tgtaatagac ggggttgtcg ttcgactggc ttttcgccag
     4141 gtcgaggtcg aaatccaggt gctggtcgga cttgcgcagc acatagaaga agcggcaggc
     4201 gtcgttgccg acgtccgcgc gcagcgtgcg cagggtcacg aactcgcccg atcgggtaga
     4261 catctgcgcc ttccagccgg agcggtagag cacggcaaac tgcaccagcg ccacctccag
     4321 agcttccgga tcgagcccca gcgcgccgag cgcgcccttg atgcgcggaa tgtagccgtg
     4381 gtggtcggcg ccccagacgt tgatcatccg ttcgaagcca cgctcgtact tgttgaggtg
     4441 gtaggcgatg tccgacgcga aataggtata aaggccattg tcgcgctgga cgacgcgatc
     4501 cttctcgtcg ccgaacgcag ttgaccggaa ccacagggcg ccgtcctgca gatagagatg
     4561 gccggccttc tgcagtcgct cgacgccacg cgtgaccaga ccggtagcaa acagcgactg
     4621 ttcggagaac catacgtcga aatgcacccc ggactcttcg agatcgctgc ggcagtccgc
     4681 gagttgctcg gtgagcacga agtcgtgcac gtaccgccac tcttcgccga gcagtcgctt
     4741 ggcattggcg atcagggcgt cgaggtgcgc ttcacggatt tgcgcggccg cggcgtcggt
     4801 gcggccgggg tcgggcaagg gcggtacgcc gtcgatgacg ccggcgggcg cccgcaggaa
     4861 gcgctcggca tgcgccgcca gcatctgcct ggccatctcg cgcacatatt cgccctggta
     4921 agcattggcc gggaaaggca gggattcatc gtggcgacgg tacagctcca gatagcgtag
     4981 ccaggtcgac aacgccagga tgtccatctg ccggccggcg tcgttgacgt agtattcact
     5041 ggtcacgtcc cagccggcga aagcgagcag cgtacacagg ctgccgccgt aagcggcgcc
     5101 acgaccgtgg ccgacgtgca gcgggccggt cggattggcg gagacgaact cgacgaggac
     5161 cttgcgtttg ccgccggtcg tcgagcggcc aaaggcctcg ccctgttcga gaacggcgcg
     5221 cacgacttcg agcttggctg ccggccgcag gtggaagttg atgaagcccg cgccggcgat
     5281 gtcggtcttg tcgaccaggg gcgaatacgg caactccgaa accagcaact gggccagttg
     5341 ccgtggattg cgctgcatcg aacgcgccag ttgcatcgcc aggttgcacg aaaaatcacc
     5401 gtgcgaggcc tgtttgggtc gctcgatatg gatgctggtg tcggcctcgt gcggcgcgac
     5461 gctgcgtaac gcggcacgca tcaggtcggc gaggtgggac ttcaggtctg aactcatcgg
     5521 ggtgcgtctg ctgtaggatt cggtcaagcc gctgattata cgatgcacga ccgagcgcgg
     5581 ggcgaaggat gcggcgcgat ggcgcgcgtt tctgtgtaaa ctccctgatt tgcccaggac
     5641 aaggccgttg ccagccgtgc gacttttccg cctcgccaaa atcctcagta ttgcgcaccg
     5701 ctacggtctc gacgagatgc tcttcgagca cgagcccagc ggtcgcctgg cggccgtgtc
     5761 gcgggtcctg cacttccggc gccgcttcga cgcgccgccg gcggtgcgcc tgcgcctggc
     5821 gctggaatcg ctggggccga tcttcgtcaa gttcggccag gtcctgtcta cgcgtcgcga
     5881 cctcctgcgt cccgacatcg ccgacgaact ggccaggttg caggaccgcg tgccgccatt
     5941 ttcaccggca ctggcgctga cgcagattga gagggcctat ggccggccgg cgagcgcggt
     6001 gttcgccgag ttcgattcga caccggtggc cagcgcctcg atcgcgcagg tccattttgc
     6061 ccgactgcga cctgaagacg gcggccagga agtggcggtc aagatcctgc gcccggaaat
     6121 gcacgcggtg atcgccaacg acctggcgct gctcgatacc ctcgccggcc tgctcgagaa
     6181 agtctggtcg gacggcaagc ggctgaagcc ccgcgaagtc gtcggcgagt tcgccaagta
     6241 cctctacgac gaactcgacc tgatgcgcga agccgccaac tgctcgcagt tgcggcggaa
     6301 tttcagcggt tcgcggctgt tgcaggtgcc cgaggtgtat tgggatcaat gcacgacgac
     6361 ggtgatggtc atggagcgca tgcgcggcgt tccgatcagc cagaccgagg cgctgctcgc
     6421 tgccggcatc gacctgccgg cgctgtcgcg cgccggcgtc gagatcttct tcacgcaggt
     6481 gtttcgcgac ggcttctttc acgccgacat gcaccccggc aacatcttca tcgccaccga
     6541 tgcggatcac cacggcagct acatcgcgct cgacttcggc atcgtcggga cgctgaccga
     6601 caccgacaag aattatctgg cgcagaactt tctggccttc ttccagcgcg actacaagcg
     6661 cgtggcgacg gcgcacatcg aagccg
//
LOCUS       DAOSIT010000052         6682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10396_length_6682_cov_9.399728, whole genome shotgun sequence.
ACCESSION   DAOSIT010000052 DAOSIT010000000
VERSION     DAOSIT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10396_length_6682_cov_9.399728"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLL14_02320"
     CDS             complement(<1..350)
                     /locus_tag="PLL14_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOG02784.1"
                     /translation="MPDCPDKAGSLSNDPFRVPWTSDFVEDHFGLSMVLNVGQARQVF
                     RWVAPSRFLMGSPPDAADRGSTELLHEVTLSHGFWLAETTCTQAFWQAVWPVNPSHFQ
                     DHPQNPVENVSWHDA"
     gene            841..3420
                     /locus_tag="PLL14_02325"
     CDS             841..3420
                     /locus_tag="PLL14_02325"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG02785.1"
                     /translation="MNDKIQPENEPPVQADPDLLVGREDRRTGLGVDALKRALLDKLV
                     YEQARFPQVATRHDCFAALAQAVRDRLLQRWVQTARTYRDQGSRTVCYLSAEFLIGPQ
                     LGNNLINLGIFDNAEQAMRELGLDLEMLLDQEEEPGLGNGGLGRLAACYLDSLATLEI
                     PAIGYGIRYEFGIFTQAIRDGRQVELTDKWLRSGSPWLIHRPSIAFEVKLGGYTEHQY
                     EATGSRRLRVQWVPGKVVRGTAWDMPVLGYGVNTPNRLRLWSAEAPETFDFAAFNAGN
                     YYQAVDAQISSETITKVLYPNDETEAGQELRLEQQYFFVSCSLQDMIRLQLQREQNLD
                     HFNEKFVVQLNDTHPSIAVAELMRLLVDEFMMDWDQAWSITRKTFAYTNHTLLPEALE
                     KWRLNLFRRVLPRHFEIICEINERFLDEVRIRFPGDDARLARMSLIDEHGPRYVRMAN
                     LAVAGSFAVNGVARLHTELLKSDVLKDFHEMWPEKFSNKTNGVTPRRFVLLANPTMST
                     LISDTIGSGWPTDMGRLRELEPYADDPAFREAWRRIKAGNKEHLVREMKRNAHVDVDP
                     ASMFDIQVKRIHEYKRQHLNLLHVVSLYKRLKDNPNLNVAPRTVIFGGKAAPGYALAK
                     MMIRLITAVADLISRDPAMRGKLQVVFYPNYNVKNAQAIFPGADLSEQISLAGKEASG
                     TGNMKFQMNGALTIGTLDGANVEMREEVGEDNFFLFGMTTPEVMELKSRGYRPSSYYE
                     ANPHLREVIDLIAAGFFTRGDREVFRPMVDNLLNHDEYMLLADFQSYIDCQARVSETY
                     LDPERWSRMSILNVARSGFFSSDRAIREYCNEIWKVKPVRIELSDLSGDDMQFRRAAA
                     GAD"
     gene            complement(3518..5476)
                     /locus_tag="PLL14_02330"
     CDS             complement(3518..5476)
                     /locus_tag="PLL14_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HOG02786.1"
                     /translation="MVPKAYPRVIIEAVEPRVDGGRFPIKRVVGEKVVVSADIYKEGH
                     DKLAAALKVRQIGHQQWQESPMTQGDNDRWYGEFTVTAIGRWEYTIEAYAERYLSWTD
                     EIGKKNIPGANLTSELLEGLVILKKSAAAAQGEDRVRMHSVIAAIESALAAGEQQGAV
                     DLGVGSVMRSMMASYPDRSEGYELAPALPIMVNRPITRFAAWYEFFPRSQSNMPYRHG
                     TLQDSIRRLREIRTMGFDVVYLPPIHPIGHSFRKGKNNSLTAVPGDVGSPWAIGNENG
                     GHMALEPQLGTWDDWDQFVGTCRELGIEIALDYVMNCSPDHPYVAAHPDWFFHRPDGS
                     IKYAENPPKKYQDVYPLNFGTTDRNGLWEEMLRIFLFWVDKGVTTFRVDNPHTKPVPF
                     WEWVIGEVHRQHPEVIFLAEAFTKPKMMRLLAKVGFAQSYTYFTWRNHKHDLSEYVSE
                     LTCGEMKEYFTGNFFANTPDILPPILQYGGRPAFIMRAVLAATLSSVYGIYSGYELCE
                     NEAIPGKEEYLDSEKYEIRMRDWKAPGNIVHIITRINQIRHDSPALHTYNNVLFLATD
                     NPNLMAYAKMNEDRSDIIVCVVNLDPFHKQVATLHVPLGTFGIGEDEQYQAHDLLSDE
                     RYRWRGPTAYVELDPSTKVAHIIKIRRW"
     gene            complement(5487..>6682)
                     /gene="glgA"
                     /locus_tag="PLL14_02335"
     CDS             complement(5487..>6682)
                     /gene="glgA"
                     /locus_tag="PLL14_02335"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOG02787.1"
                     /translation="TREYPPYVYGGAGVHVEYLSGELAKLMHVEVRSFGDQDTVREGV
                     RVRGFPFGKGSFAHVDKKLSGALDTFETNLETLSEQVDADLVHVHTWYAHLGGILAKT
                     LYGIPLVHTVHSLEPLRPWKREQLGCGYDMSSWIERTSLGMADAVIAVSQGTKDDILA
                     HFDIDPAKITVIYNGINVEQYHPTEDTTALEKYGVDPQKPIVLFVGRITRQKGIVHLV
                     NAVQYINPTAQIVLCAGAPDTREVLAEMEAAIRHIRHEGFNNVIWIQEMVSKEEAIEL
                     YSHATVFCCPSIYEPFGIINLEAMACRTAVVASSVGGIKEVVVDGVTGYLVPLEQWHV
                     APFEPVNPDVFSRDLATAINKILDDPELAESMAAAGQQRAREVFSWSSIAQQTKRLYD
                     SLVKF"
BASE COUNT         1296 a         1967 c         2025 g         1394 t
ORIGIN      
        1 gcgtcgtgcc aactgacatt ctccacgggg ttctgcgggt gatcctggaa gtggctcgga
       61 ttcaccggcc atacggcctg ccagaatgcc tgcgtgcatg tggtttcggc gagccagaag
      121 ccgtggctca gtgtgacttc gtgcagtaat tcagtactgc cccgatcggc agcgtcaggc
      181 ggtgaaccca tcaggaaacg ggacggtgcc acccaacgga agacctgtcg tgcctggcct
      241 acgttcagga ccatcgacag gccaaagtgg tcttcgacga agtcgctggt ccacggtacc
      301 ctgaagggat cattggacaa gctgccggcc ttgtcagggc aatctggcat catcagtttc
      361 tccatatttc cgctctcagc gaggcgatgt ccgtggtgtg gtcgtgtctg ctgccttgcg
      421 acaccgtcag tgggtatcca gggcgcgttc gacgggcggg ggaattcggt ctatgaggca
      481 ggggcatggt cctgtatcac tcgacgatgg ccatcgcagg ccagctaccg atgctgggct
      541 ttcagtgtac ccgtccagtg acctgccggg aaggcctcgc tcttgtcaac ccggcacggc
      601 cgagtggcca tctgtgccca cgtcacgagg ccagggtacc cgcccatagt tcctcgtgaa
      661 ccgctgtcgg gtctcgtttg ggcattcccg agccagcatg caagatcaga tttttctgga
      721 cagcagcccc ctgtgcgtgc ttgcaaacaa tgctatagac tcgccgctga cgtggatcgt
      781 cagacaagga tcaacgaatt ctcatactgg aatgaacggc acgaacacgg gggtgttgac
      841 atgaacgaca agatacaacc tgaaaatgaa ccgcctgtgc aggcggatcc cgatcttctg
      901 gtggggcgcg aggatcggcg tacagggctg ggtgtggatg cattgaagcg tgcgttgctc
      961 gacaagctgg tgtatgagca ggcacgcttt ccacaagttg cgacgcggca cgactgcttt
     1021 gccgcgctcg cccaggccgt gcgcgaccgc ctgctgcaac gctgggtgca aaccgcacgc
     1081 acctaccgtg accagggcag ccgtaccgtc tgctacctgt cagccgagtt cctgattggt
     1141 ccgcagctcg gaaacaacct gatcaatctc ggcatcttcg acaacgccga gcaggcgatg
     1201 cgggaactcg gcctcgatct ggaaatgctg ctcgatcagg aggaggagcc tggccttggc
     1261 aacggcggtc tcggacgcct ggccgcgtgt tatctggact ccctggcaac gctcgaaatt
     1321 cccgccattg gttatgggat ccgctacgaa ttcggcatct tcacgcaggc gatccgcgat
     1381 ggccggcaag tagaactgac cgacaagtgg ttacgttccg gaagtccctg gctgattcac
     1441 cgtccgagca ttgctttcga ggtcaagctc ggtggctaca ccgagcatca gtacgaagcg
     1501 acgggcagtc gccgcctgcg cgtgcaatgg gtgccaggga aagtcgtacg tggtacagcc
     1561 tgggatatgc cggtgcttgg ctacggggtg aacacaccca accggctcag gctgtggagc
     1621 gccgaggcgc ccgaaacctt cgattttgcc gctttcaacg ccggcaatta ctaccaggcg
     1681 gtcgatgccc agatctcctc ggagaccatc accaaggtgc tatacccaaa cgatgaaact
     1741 gaagccgggc aggagttgcg gctcgagcag cagtactttt tcgtttcctg ctcactgcag
     1801 gacatgattc gtctgcaact gcaacgtgaa cagaaccttg atcacttcaa tgaaaaattc
     1861 gtcgtccagc tcaacgacac gcatccgtcg atcgccgtgg cggaactgat gcgcctgctg
     1921 gtcgatgagt tcatgatgga ttgggatcag gcctggtcaa tcacccgcaa gacctttgcc
     1981 tacaccaacc atacgctgct gccggaggcg ctcgagaagt ggcggctgaa tctgttccgg
     2041 cgcgtgctgc cgcgacattt cgaaatcatc tgtgagatca acgagcgctt cctcgacgag
     2101 gtgcgcattc gctttccggg tgacgacgcg cgcctcgccc ggatgtcgct gatcgacgaa
     2161 catgggccac gctatgtgcg catggcgaat ctggcggttg cgggcagttt cgcggtaaac
     2221 ggggtggcga ggttgcacac cgaactgctg aaatccgatg tcctcaagga cttccacgaa
     2281 atgtggccgg aaaaattcag caacaagacc aatggcgtga cgccgcggcg cttcgtcctc
     2341 ctggcgaatc cgacgatgtc caccttgatc agcgacacga ttggcagcgg ctggcctacc
     2401 gatatgggtc ggctgcggga actcgaaccg tacgccgacg acccagcctt ccgcgaggcc
     2461 tggcggcgga tcaaggccgg caacaaggaa cacctggtgc gcgagatgaa gcgcaacgcg
     2521 catgtggatg tcgacccggc gtcgatgttc gacatccagg tcaaacgcat tcacgaatac
     2581 aagcggcaac acctcaacct gctgcacgtg gtttccctgt acaagcgcct caaggacaat
     2641 ccgaacctga atgtcgcgcc acgcacggtg attttcggcg gcaaggctgc tcccggctac
     2701 gcgctggcca agatgatgat ccggctgatt accgccgtcg ccgatctgat cagtcgcgac
     2761 cccgcgatgc gcggcaagtt gcaggtcgtc ttctatccga actacaacgt caagaatgcc
     2821 caggcgatct tccctggcgc cgatctgtcc gagcagattt cgcttgccgg caaggaagca
     2881 tcagggaccg gcaacatgaa gttccagatg aacggtgcgc tcaccatcgg tacgctcgac
     2941 ggtgccaacg tcgagatgcg tgaggaggtt ggtgaagaca acttcttcct gttcgggatg
     3001 accactcccg aagtcatgga gctcaagagc aggggttacc ggcccagcag ttactacgag
     3061 gccaacccgc acctgcgcga ggtcatcgac ctgattgccg ccggcttctt cactcgcggc
     3121 gatcgcgagg tcttccggcc gatggttgac aacctgctca atcacgacga gtacatgctg
     3181 cttgccgact tccagtcgta tatcgattgt caggccaggg tttcggaaac ctacctggat
     3241 cccgagcgct ggtcgagaat gtccattctc aacgttgccc gttccgggtt cttctcttcc
     3301 gatcgggcga tccgtgaata ctgcaacgag atatggaagg tgaagccggt gcgcattgaa
     3361 ctcagtgact tgtcgggaga tgacatgcag ttcagacgcg ccgcagccgg agcagattga
     3421 gcggctgccg gcagggcagg ggcggcagcc ccgccctgct gccctgtccc tctccgttcc
     3481 gcgaacgcat gaccgcgtgc gcccccggcg ccgccttcta ccagcgacgg atcttgatga
     3541 tgtgtgccac cttggtgctc ggatcgagtt cgacgtaggc ggttggcccg cgccagcggt
     3601 agcgctcgtc cgagagcagg tcgtgcgcct gatactgttc gtcctcgccg ataccaaagg
     3661 tgcccagcgg cacgtgcagc gttgccactt gtttgtggaa cggatcaagg ttgaccacgc
     3721 agacgatgat gtccgacctg tcctcgttca tcttggcata ggccatgaga ttggggttgt
     3781 cggtagcgag gaagagcacg ttgttgtacg tgtgcagggc cggcgaatca tggcgaatct
     3841 ggttgatgcg ggtgatgatg tggacgatgt tgccgggcgc tttccagtcg cgcatcctga
     3901 tctcgtactt ctccgagtcg aggtattcct ccttgccggg aatggcttcg ttctcgcaca
     3961 gctcgtagcc gctgtagatg ccgtaaacgc tggacagcgt ggctgccagt acggcgcgca
     4021 tgatgaacgc cgggcgaccg ccgtactgga gaatcggcgg caggatgtcg ggagtgttgg
     4081 cgaagaagtt gccggtgaag tactccttca tttcgccaca ggtcagctcg ctcacatact
     4141 cgctgaggtc atgcttgtgg ttgcgccagg tgaagtaggt gtaggactgg gcgaagccta
     4201 cctttgccag cagcctcatc atctttggct tggtgaaggc ttcggccaga aagatgactt
     4261 cggggtgctg ccgatggact tcgccgatca cccattccca gaacggcacg ggtttggtgt
     4321 gcgggttgtc gacgcggaaa gtggtcaccc ccttgtctac ccagaacagg aagatcctga
     4381 gcatttcctc ccacaagccg ttgcggtcag tcgtgccaaa gttgagcgga tagacgtcct
     4441 ggtatttctt gggcgggttc tcggcgtatt tgatcgatcc gtcggggcga tggaagaacc
     4501 agtccggatg cgcggccacg tagggatggt cgggtgagca gttcatcaca taatcgagcg
     4561 caatctcgat gcccagttca cgacaggtgc cgacaaactg gtcccagtcg tcccaggttc
     4621 cgagctgtgg ttcgagcgcc atgtgaccgc cattctcgtt gccgatcgcc cacgggctgc
     4681 cgacatcgcc cggaacggcc gtcagactgt tgttcttgcc cttccggaaa ctgtgtccga
     4741 tgggatggat cggcggcaga tacacgacat cgaaacccat cgtcctgatc tcgcgcagac
     4801 gacggatgct gtcctgcagg gtgccatgcc ggtacggcat attggactgc gagcgcggga
     4861 agaattcgta ccacgcggca aagcgggtga ttggccgatt gaccatgatc ggcaatgctg
     4921 gcgctagttc gtaaccctcg ctgcgatcgg ggtagctggc catcatgctg cgcatcacgc
     4981 tgccgactcc caggtctaca gcgccctgct gctcgcctgc agcgagtgcg ctctcgatcg
     5041 cggcgatgac gctgtgcatg cgtacgcgat cctcaccttg cgctgcggca gcagacttct
     5101 tgaggatgac cagcccctcc agcagttcgc tggtaaggtt ggcacccggt atgttctttt
     5161 tgccgatttc gtcggtccat gaaaggtagc gctcggcgta agcctcgatg gtgtactccc
     5221 atcgaccgat cgcggtgacg gtaaactcac cgtaccagcg gtcattgtca ccctgcgtca
     5281 tcgggctttc ctgccactgc tggtggccga tctggcgcac cttgagggcg gcagccagct
     5341 tgtcgtggcc ttccttgtaa atgtcggcgc tgacgaccac tttctcgccg accacgcgct
     5401 tgatggggaa gcgtccgccg tcgactcgcg gctcgactgc ttctatgatc acgcggggat
     5461 atgctttggg aaccatcacc atgctcctaa aatttcacca gcgaatcgta gagtctcttg
     5521 gtttgctgcg cgatgctcga ccaactgaaa acttcgcgtg cgcgctgctg gccggctgcg
     5581 gccatggact cggcaagctc gggatcgtcc aggatcttgt tgatggccgt cgccaggtca
     5641 cgcgaaaaga catccgggtt gacgggctcg aacggtgcca catgccattg ctcgagcggc
     5701 accaggtagc cggtgacgcc atcgacgacg acctccttga tgcccccgac tgaactcgcc
     5761 acgacggccg ttcgacaggc catggcttcg agattgatga tcccgaaagg ctcgtagatg
     5821 gacggacaac agaagaccgt ggcgtgtgaa tacagttcga tcgcttcctc cttgctgacc
     5881 atctcctgaa tccagatcac gttgttgaaa ccttcatggc gaatgtgcct gatcgcggct
     5941 tccatctccg cgagcacctc cctggtatcg ggtgcgccgg cacagagcac gatctgagct
     6001 gtggggttga tatactggac cgcattgacc agatggacga tgcctttctg gcgcgtgatc
     6061 cggccgacga aaagaacgat cggtttttgc ggatcgacgc cgtatttttc cagcgcggtc
     6121 gtgtcctcgg taggatggta ctgctcgaca ttgatgccgt tgtagatcac cgtaatcttc
     6181 gccgggtcga tgtcgaaatg cgccaggatg tcgtccttgg tcccctgcga gacggcaatg
     6241 accgcatcgg ccataccgag tgaagtgcgt tcgatccacg acgacatgtc gtagccacag
     6301 ccaagctgct cgcgcttcca cggacgcagc ggctccagcg aatggaccgt atgcaccagc
     6361 gggatgccgt acagggtctt tgccaggata cccccgaggt gtgcatacca ggtgtgcaca
     6421 tgcaccagat cggcgtcaac ctgttcgctg agggtctcga ggttggtttc aaaggtgtcg
     6481 agtgcgccgc tgagtttctt gtcgacgtgg gcaaaactgc ccttgccgaa cgggaaaccc
     6541 cggacccgca caccctctct gacggtatcc tgatcaccaa aggagcggac ctcgacatgc
     6601 atcagcttgg cgagttcgcc agacagatac tcgacgtgaa caccggctcc gccgtagacg
     6661 tagggagggt attctcgcgt ca
//
LOCUS       DAOSIT010000053         6675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10407_length_6675_cov_6.684894, whole genome shotgun sequence.
ACCESSION   DAOSIT010000053 DAOSIT010000000
VERSION     DAOSIT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_6675_cov_6.684894"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..654)
                     /locus_tag="PLL14_02340"
     CDS             complement(73..654)
                     /locus_tag="PLL14_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG02788.1"
                     /translation="MSIKERLKSLGKLLLGGYEINRMYHSPPLHEHFVCPDGAHFATI
                     ETTRQVAGCEYPEISRHAWYTSKPGHSYGAWENGRLVCFCTYWTNTEDNLPRRFRNIR
                     DDEIVMVDLITAECARGKGFSVQMMAFSASELKKLGYRRCLTWIWHSNHPSIRAFEKA
                     GWIYDYFLIELPWRGEKDRIRIRLPRWVARLFG"
     gene            complement(651..>1394)
                     /locus_tag="PLL14_02345"
     CDS             complement(651..>1394)
                     /locus_tag="PLL14_02345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG02789.1"
                     /translation="ALSEDDMLQRFLRAYRRHQLKDIPGLILKNVRHAWTHFSEPSTP
                     NPSEFDATYHVETDSIRETGSLDIDSPNARHAVRYEPSPSGMVRSILSTLGLDWRRYT
                     FVDFGAGKGRVLLLAAELPFKSIIGVEFSPELAELASNNIARYLAQAGSSARISCLCL
                     DAVDFRFPEDPLVCYFYNPFGYPVLERVLSNLLASHARSARDIYVIYVHPVHQAEIKR
                     QSPALVPIREDEGLVVFHLGKDMGGQSIR"
     assembly_gap    1395..1494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1552..1800
                     /locus_tag="PLL14_02350"
     CDS             1552..1800
                     /locus_tag="PLL14_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HOG02790.1"
                     /translation="MNISSVLVYTRPQDLQLVRENLSALTGVEVHAASENGRLIVIIE
                     ADSVQAVADIFAQINQQPGVLAASMVYHQFEPDPDEEA"
     gene            1808..4336
                     /gene="napA"
                     /locus_tag="PLL14_02355"
     CDS             1808..4336
                     /gene="napA"
                     /locus_tag="PLL14_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HOG02791.1"
                     /translation="MTLTRRDFIKSQAVAAAACVAGMSVPGLAQAAPAASAREDGVRW
                     DKGVCRYCGTGCGVLVGTRDGRIVATQGDPDAPVNRGLNCIKGYFLSKIQYGKDRLTT
                     PLLRMKNGKFDKGGEFTAIAWDEAFEIMADKCKAALRKGGPRNIAMFGSGQWTIWEGY
                     AAAKLMKAGLLSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEHADAFVLWGSN
                     MAEMHPILWSRVTDRRLTGKNVKIHVLSTFSHRSTELADNELIFKPQSDLAILNYICN
                     YIIQNKAVNQEFVARNVNFRKGVTDIGYGLRANHPLEKAAMNNGYPGEDGKPRGNPAG
                     ASPISFDEFKAFVAEYTLDKAHELSGVPKERLEELARAYADPKTRVTSFWTMGFNQHT
                     RGTWVNNMIYNVHLLVGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADMLVTNP
                     KHREASEKLWKLPAGTIPDWIGAHAVAQSRLLKDGKINFYWTTTTNNMQAGPNVNDEI
                     FPGWRNPDNFIVVSDAYPTVSAMTADLILPSAMWMEKEGAYGNAERRTQFWRQQVKAP
                     GQARSDLWQYIEFSKRFKTDEVWPAALLEKNPEYKGKTLYDVLYANGQVNKFPLDQTY
                     KGFDNDESRACGYYLQKGLFEEYAAFGRGHAHDLAAFEVYHKARGLRWPVVDGKETLW
                     RFREGYDPYVKPGEGVKFYGHPDGKAVIFALPYQPAAEMPDKDYDLWLCTGRVLEHWH
                     TGSMTRRVPELYKAMPDAWIYMHPEDAKRRGLQRGDVVKTITRRGEIQLRVETRGRNK
                     PPVGLVFVPFFDEHRLVNKLTLDATCPISKETDFKKCACKVVKA"
     gene            4424..>5241
                     /gene="napG"
                     /locus_tag="PLL14_02360"
     CDS             4424..>5241
                     /gene="napG"
                     /locus_tag="PLL14_02360"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HOG02792.1"
                     /translation="MFPEKNHARRVPSRAENAPALARRQFVFDSARLLCGVGVLGLCL
                     AGYARKALAKPPMAIRPPGAGPEEDFLGACIRCGMCVRDCPYDILELARPESLVATGT
                     PYFTARSGPCEMCEDIPCVRACPTGALDHHLTDINKSRMGLAVLSDQETCLNFLGLRC
                     DVCYRVCPVIDKAITLELRPNTRTGRHSMFLPTVHSEHCTGCGKCEKACVTDEASIRV
                     LPPRLVKGSLGEHYRLGWEEKTRAGGHSLVEDKGIIDLPDRLPEGTTLEGHFDPG"
     assembly_gap    5242..5341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5342..6153
                     /gene="napH"
                     /locus_tag="PLL14_02365"
     CDS             <5342..6153
                     /gene="napH"
                     /locus_tag="PLL14_02365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HOG02793.1"
                     /translation="AILALFLLGPLAAVWVVKGNLAYSLTLDTLPLTDPYVLLQSVLA
                     GHLPEKSALIGALIVIIFYILVGGRVYCAWVCPLNLVTDLAGWLRYRLGIKGSVHVAR
                     SSRYGVLMMTLLLAALTGTLVWELVNPVSMLQRGLIFGLGAAWTVVLAVFLFDLFVMS
                     RGWCGHLCPVGAFYSVLGKWSPLRISASKRAACNDCMDCFEVCPEPQVIRPALKGAGK
                     GVGPVILAANCTNCGRCIDVCSKDVFTFGLRFNNPPGNGVQTATRHATSGN"
     gene            6205..6672
                     /locus_tag="PLL14_02370"
     CDS             6205..6672
                     /locus_tag="PLL14_02370"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HOG02794.1"
                     /translation="MKNLLKLAAAMTASCLTSLAFAADTTTSLRGIDVVAPDQAPEVK
                     NYAGRRPGTETPVARTFSTQPPVIPHAVENFDEINLDGNQCMDCHSGATYQKKKAPKI
                     GDSHFIDRQGNKLTEASASRYNCVQCHVPQVDAPPLVDNTFKGDAARKPVRKN"
BASE COUNT         1316 a         1931 c         1986 g         1242 t
ORIGIN      
        1 ctgttctcaa aaccgcgggc caacgccaac aaccgctgac ccgcagacca ggcgggtacg
       61 cttgctgctg ggtcagccaa acaatcgggc gacccagcgt ggcagcctga tacggatgcg
      121 gtccttctcg ccgcgccatg gcagttcgat caggaagtaa tcgtagatcc agcctgcttt
      181 ttcgaacgca cggatcgacg gatggttcga atgccagatc caggtcaagc atctgcggta
      241 tcccagcttc ttcagttcgc ttgcggaaaa ggccatcatc tgtacggaga atcccttgcc
      301 acgggcacat tcggcggtga tcagatcgac catcacgatt tcatcgtcac ggatgttccg
      361 gaagcgacgg ggcagattgt cttcggtatt cgtccagtag gtgcagaagc acaccagccg
      421 accattttcc caggcgccgt agctgtgccc cggcttgctg gtgtaccagg catgtctgct
      481 gatctccgga tactcgcatc cggctacctg ccgagtcgtc tcgatggtcg caaagtgcgc
      541 gccgtccggg cagacgaaat gctcgtgcag gggcggagag tggtacatcc gattgatctc
      601 gtagccgccc agcagaagct tgcccaggct cttgagacgt tccttgatgc tcatcggatc
      661 gattgccccc ccatgtcctt accgagatga aagaccacca acccttcatc ctcgcgtatc
      721 ggcacgaggg cagggctttg acgtttgatt tccgcctggt gcaccgggtg gacatagatc
      781 acgtagatgt cccgggcgga gcgcgcatgg ctggccagga ggttggacag caccctctcg
      841 agcaccgggt atccgaaggg attgtagaaa tagcaaacca gagggtcttc gggaaagcgg
      901 aaatcgaccg cgtccaggca cagacaggag atgcgcgcgc tgctgccggc ttgcgccaga
      961 taccgggcga tgttgttgct ggcgagttcg gcgagctcgg gggagaactc gacaccgatg
     1021 atgctcttga acggcaactc cgccgccagc aacaggaccc ggccctttcc cgcaccgaag
     1081 tcgacaaacg tataccgacg ccaatcgagg ccgagcgtgc tcaggatgga cctgaccatc
     1141 ccggagggtg acggttcgta tcggactgca tgcctggcgt tgggcgaatc gatgtccaga
     1201 gagcccgtct cgcggatact gtcggtttcg acgtgatagg tggcgtcgaa ttccgatggg
     1261 ttgggtgttg atggctcgct gaaatgcgtc caggcgtgac ggacgttctt caggatcaag
     1321 cccggtatgt ccttgagttg gtgccgccga taggcacgca gaaagcgctg taacatgtcg
     1381 tcctcggata acgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccggc
     1501 ggctgccggt gattgcgtcg gcagttctgc gaccaggagt atctcgactt catgaacatc
     1561 tccagcgtgc tggtctacac ccgccctcag gaccttcaac tggtacggga aaacctgtcg
     1621 gcgctcactg gtgttgaagt gcatgcggca agcgagaacg gccgcttgat cgtgatcatc
     1681 gaggctgact ccgtgcaagc tgtggccgac atctttgcgc agatcaatca gcagccgggc
     1741 gtgctcgcgg cgtcaatggt ttaccaccag ttcgaacccg atcctgatga ggaagcatga
     1801 gatgaaactg actttgaccc gtcgtgactt tatcaagagc caggcggttg ccgcggccgc
     1861 ctgcgttgcc gggatgagtg tgccggggct tgcacaggcg gcgccggcag cgtcggcgag
     1921 ggaggacggc gtgcgctggg acaagggcgt ctgccgctac tgcggcacgg gttgcggcgt
     1981 gctggtggga acccgggacg gccgtatcgt ggctacgcag ggtgaccccg acgccccggt
     2041 gaaccggggg ctgaactgca tcaagggtta cttcctgtcg aaaatccagt acggcaagga
     2101 tcgtctcacc acgcccctgc tgcgtatgaa aaacggcaag ttcgacaagg gcggtgagtt
     2161 tacggcgatt gcatgggatg aggccttcga gatcatggcc gacaagtgca aggccgcgct
     2221 caggaaaggc gggccgcgca atatcgccat gttcggttcc ggccaatgga cgatctggga
     2281 aggctatgcg gcggccaaac tgatgaaggc cggcctgctg agcaataacc tcgatcccaa
     2341 tgcgcggcac tgcatggctt cggcggtagc cggcttcatg cgcaccttcg gcatcgacga
     2401 accgatgggc tgctacgacg acatcgagca cgccgacgcc ttcgtactgt ggggctcgaa
     2461 catggccgag atgcacccga ttctgtggtc gcgggtgacc gaccgtcgtt tgaccggcaa
     2521 gaacgtcaag attcacgtgc tctcgacatt tagccatcgc tcgacagagc ttgctgacaa
     2581 cgaactgatc ttcaagccgc agagcgacct ggcgatcctc aactacatct gcaactacat
     2641 catccagaac aaggcggtca atcaggaatt cgtggcccgt aatgtgaact tcaggaaggg
     2701 agtgaccgac atcggctatg gcctgcgcgc caatcatcca ctcgagaagg ccgccatgaa
     2761 caacggctat ccgggtgagg acggcaagcc gcggggcaac cctgcgggcg ccagcccgat
     2821 cagctttgac gagttcaagg ctttcgtcgc cgaatacacc ctcgacaagg cacacgaact
     2881 atccggcgta cccaaggaaa gactcgaaga actggccagg gcctacgccg acccgaagac
     2941 cagggtgact tcgttctgga ccatgggttt caaccagcac acgcgcggca cctgggtcaa
     3001 caacatgatc tacaacgtgc acctgctggt gggcaagatc tccgaaccgg gcaacagccc
     3061 gttttcgctg accggccagc cttcggcctg cggcaccgca cgcgaagtcg gtactttctc
     3121 gcatcgcttg ccagccgaca tgctggtcac caatccgaag catcgggaag ccagcgagaa
     3181 gctctggaaa cttcccgccg gtaccatccc cgactggatt ggcgcacatg ccgtggcgca
     3241 gagccgcctg ctcaaggacg gcaagatcaa cttctactgg acgacgacga ccaacaacat
     3301 gcaggccggg ccaaacgtca acgacgagat tttccctggc tggcgcaatc cggacaactt
     3361 catcgttgtt tccgatgcct atccgaccgt ctcggcaatg accgccgacc tgatcctgcc
     3421 atcggcgatg tggatggaga aggaaggtgc ttacggcaac gccgaacggc gcacgcagtt
     3481 ctggcgtcaa caggtcaagg caccgggtca ggcgcggtcc gatctctggc agtacatcga
     3541 gttctcgaag cgcttcaaga ccgacgaggt gtggccggca gcgctgctgg agaaaaaccc
     3601 cgagtacaag ggcaagaccc tttacgacgt gctctatgcc aacggtcagg tgaacaaatt
     3661 cccgctcgat cagacctaca aaggcttcga caacgacgaa tcgagggctt gcggctatta
     3721 cctgcagaag ggtctgttcg aggaatatgc cgccttcggt cgtggccatg cccacgatct
     3781 ggcagcgttc gaggtttatc acaaggcacg tggcttgcgc tggccggtgg tcgatggcaa
     3841 ggaaacgttg tggcgctttc gcgagggcta cgatccgtat gtcaaaccgg gcgagggcgt
     3901 caagttctat ggccatcccg atggcaaggc ggtgatcttt gccttgccct accagccagc
     3961 cgccgaaatg ccagacaagg actacgatct ctggctgtgc accgggcgcg tgctcgaaca
     4021 ctggcatacc ggttcgatga cgcggcgcgt cccggagttg tacaaggcaa tgccggatgc
     4081 gtggatctac atgcatccgg aagatgcgaa aaggcgtggg ctgcaacggg gcgatgtggt
     4141 caagaccatc acccgtcgcg gcgagatcca gttgcgggta gaaacccgcg gccgcaacaa
     4201 gccacccgtc ggtctggttt ttgtgccctt cttcgacgaa caccggttgg tgaacaaact
     4261 gacgctcgat gccacctgtc cgatttcgaa ggagaccgat ttcaagaaat gcgcatgcaa
     4321 ggtggtcaag gcctgatcga cacgccagac ccggacctgc ctgggtgggc tgatcaccgt
     4381 ccgtcccccg gctgaccacc gtctgctttc tgcccacacc atcatgtttc ccgagaaaaa
     4441 ccacgccagg cgtgtgccgt cccgtgccga aaacgcaccg gcgctggcgc gtcggcaatt
     4501 tgtcttcgac tccgcccgtc tgctctgtgg cgttggcgtc ctcgggctgt gtcttgcggg
     4561 atacgccagg aaagctctgg cgaagccgcc gatggctatt cgcccgccgg gtgccggccc
     4621 ggaagaggac tttctcggtg cctgcatccg ttgcggcatg tgcgttcgcg attgccctta
     4681 cgacattctc gaactcgcca ggccggaatc gctggtcgcc accggtacgc cctacttcac
     4741 ggcgcgcagc gggccctgtg agatgtgtga ggacatcccc tgtgtcaggg cctgtccgac
     4801 cggcgccctc gaccatcacc tgacggacat caacaaatcc cgcatgggcc tcgctgtcct
     4861 gtcggatcag gaaacctgcc tgaattttct tggcctgcgc tgcgatgtct gttatcgcgt
     4921 ttgcccggtg atcgacaagg cgatcaccct cgagttgcgc ccaaataccc gtactggtcg
     4981 gcacagcatg ttcctgccga cagtgcactc cgagcactgc accggttgcg gcaagtgcga
     5041 aaaggcgtgc gtgaccgacg aggcctcgat cagggtcttg ccgcccagac tggtcaaggg
     5101 ttcactgggc gagcactacc gcctcggttg ggaagagaag acgagagcgg gtggccattc
     5161 gctggtcgag gacaagggaa tcatcgatct gcctgatcga cttcccgagg gaacgacgct
     5221 ggagggccac ttcgatccag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 ntggccatcc ttgccctgtt cctgctcggg ccgctcgcgg cggtatgggt ggtcaaggga
     5401 aatctggcgt acagcctgac gctcgacacc ctgcctttga ccgatcctta cgtgctgctg
     5461 caatcggttc tcgccggcca cctgcccgag aagagcgccc tgatcggcgc actgatcgtc
     5521 atcatcttct acatccttgt cggcggccgt gtctattgcg cctgggtttg tccgctcaat
     5581 ctggtgaccg atctcgcggg ctggttgcgt taccggctgg ggatcaaggg cagcgtgcat
     5641 gtcgcccgca gcagtcgcta cggggtcctg atgatgacct tgctgctggc agcgctcacc
     5701 ggtaccctcg tatgggaatt ggtcaacccg gtatcgatgc tgcagcgtgg gctgatcttc
     5761 gggctgggag cggcatggac agtggtgctg gcggtctttc tgttcgacct gttcgtgatg
     5821 agtcgtggct ggtgtgggca tctgtgcccg gtcggtgcct tctacagtgt gttgggcaaa
     5881 tggagcccac tacggatcag cgcgtcgaaa cgcgcggcct gcaacgattg catggactgc
     5941 ttcgaggtat gcccggaacc gcaggtcatt cgtccggcac tgaagggagc gggcaagggc
     6001 gtcggcccgg tcattctggc cgccaactgt accaattgcg ggcgttgcat cgatgtctgt
     6061 tccaaggatg tcttcacgtt cggactgcgt ttcaacaacc cgcccggcaa tggggtgcag
     6121 accgcaacac ggcatgcgac aagcggcaac tgaatcacta gagaagcatc gaactcatcc
     6181 tttgacgtgg aggaaacgcc gatcatgaaa aacttactga aacttgcggc cgccatgacg
     6241 gcaagctgcc tgacgtctct ggcgtttgcc gcagacacga cgacttcact gcgcggtatc
     6301 gacgtcgtcg cgccagacca ggcacccgaa gtcaagaact atgccggcag gcgcccgggg
     6361 acggaaaccc cggtggcgag gaccttcagc acgcagccac cagtgattcc acacgcggtg
     6421 gagaacttcg acgaaatcaa tctcgatgga aaccagtgca tggattgcca ttcgggggcc
     6481 acataccaga agaaaaaggc accgaaaatt ggggacagcc acttcattga tcgtcagggc
     6541 aacaagctga ccgaagcgag cgcgagccgg tacaactgcg tgcagtgtca tgtcccgcaa
     6601 gtcgatgcgc cgccgctggt ggacaacact ttcaagggtg acgcagccag gaagcctgtc
     6661 aggaaaaact gaagc
//
LOCUS       DAOSIT010000054         6630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10509_length_6630_cov_6.801369, whole genome shotgun sequence.
ACCESSION   DAOSIT010000054 DAOSIT010000000
VERSION     DAOSIT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_6630_cov_6.801369"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="PLL14_02375"
     CDS             <1..1658
                     /locus_tag="PLL14_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOG02795.1"
                     /translation="GRVAEVAARFTLDAMPGKQMAIDADLNAGLIGEDEARKRRATIA
                     QEADFFGSMDGASKFVRGDAVAGILIMLINVVGGLFVGVLQHNLDIGTAAKTYTLLTI
                     GDGLVAQIPALIISIAAGMVVTRVGDDEDVSQQFISQLFSNPKLLFLTAAIIGMLGLI
                     PGMPNFVFLLLASALGAMAWRLEKRSQLAVPVPVTAAPVAPRETQEASWADVSPLDVL
                     ALEVGYRLIPLVDKSQDGELLRRIRGIRKKFAQEVGFLVSPVHIRDNLELKPNAYKIL
                     LKGVEIGDGEAFPGNLLAINPGRVAGQIPGTVTKDPAFGLPAVWIDPALREQAQAYGY
                     TVVDASTVAATHLNHLILSHAAELLGRQETQALLDHLAKEMPKLVEDVVPKVLSLGVV
                     QKVLRNLLEEGVHLRDMRTVIETLADVAPRSQDPEVLTAQVRTALGRSIVQELYPGGA
                     EMQVMSLDPQLERLLLQAVAGGGDSAGIEPGLAETLVREAVAAAQRQEEVGLPTVLLV
                     PGNLRTLLSRFLRRSISQLKVLAHAEVPENRIIKVTSIIGQRV"
     gene            1655..3040
                     /gene="flhF"
                     /locus_tag="PLL14_02380"
     CDS             1655..3040
                     /gene="flhF"
                     /locus_tag="PLL14_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOG02796.1"
                     /translation="MNVRKFIAATARDALRKVKETLGPDAIILSNRGIPGGVEIMAVA
                     ARDMDMIVPTSEREASPARAMDYPTVSSVDRGPATAPEPPSVRGASPAAGQQSAAARS
                     LPPPLARPLAPPLERPQSSRSLPPLERPRPQAESMAKGDAEVVPAAVMDEIRSLRRIV
                     EQQLAGFAWGESARSEPVKTEILRQMLDAGFSPHFARGLLADLPRDLDSVQALAWVRG
                     GAERSMLTISSETDIVHRGGIYALVGPTGVGKTTTTAKLAARCVLRHGARKLALVTTD
                     GYRIGAHEQLRIYGRILGVSVHLARDAKDLRATLRDLQHTHMVLIDTMGMSQRDRMVS
                     EQVAMFGDCNVKSLLLLSATSRGDTLDDVVRAYSGLDLAGCILTKVDEAASLASSLDV
                     IIRHGLLMYYVSNGQRVPEDLHLPNRPYLLHRAFKDLPETSPHRLAGVEPGLMMASST
                     VSSFTAGGQRA"
     gene            3033..3917
                     /locus_tag="PLL14_02385"
     CDS             3033..3917
                     /locus_tag="PLL14_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG02797.1"
                     /translation="MPEFRGDQAAGLRRLLGRQSLRIVTFAAGSIGVGKSVAVANLAA
                     SLARQGREVLVVDENTEDGIAAYYGVLARYDLQQVIDREKSLSEVILSVAPGIRVLPV
                     ARVVRHLARLSDAEQRILLGCLTEIGPPADVLLVDTSLDHPLGFSPLGLAAHETVIVV
                     SPSSAAITEAYALIKKVSLGYARQDFRILVNKARAAREARAIYDNIAHVTGSRGLARL
                     DYAGYVPVDEHLRQASRLCQPVSALFPDSPAAKAYQIVANDLLNWPGADDEIGGLEHF
                     VQQLLLLSQRIEPIAIYA"
     gene            3935..4681
                     /locus_tag="PLL14_02390"
     CDS             3935..4681
                     /locus_tag="PLL14_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOG02798.1"
                     /translation="MYNAAGQLNKDQLVQRFAPLVRRIACHLMARLPASVQLDDLVQN
                     GMLGLLDAIGRFEAGMGAQFEVYAAQRVRGAMLDGLRENDWLPRSLRHNCRRIEVAIA
                     QLEQENGRAPSEKELADALGMGLADYQKMLQDARGHQLVYLEDLVTERGDDFLERHLA
                     DDAADPAKLFEEGNLRQLLVQAIELLPERERLMMALYYEQDLNLREIGAVLGVTESRA
                     CQLHSQAVLRLRARLFGGNVTKTTKVARHG"
     gene            4674..5414
                     /locus_tag="PLL14_02395"
     CDS             4674..5414
                     /locus_tag="PLL14_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HOG02799.1"
                     /translation="MDRISVFGLLIGLLAIIGGQILEGGHIGSLSQPTAMLIVVGGTL
                     GAVMLQSPYATFVRGIRMVRWIWYPPVVNHLQLIQQVSGWSQVSRREGLLALDPIVNE
                     LKDEFTRKGLQLVVDGAEPDRLREVMEVEISTYEQEMKLSARIWEAAGGYSPTIGILG
                     AVMGLIQVMENLSDPSKLGAGIAVAFVATIYGVGLANLVFLPMANKLKAHINRLIVQR
                     EMIVDGLVGIANGDNPRIIESRLRGYIF"
     gene            5537..6370
                     /gene="motD"
                     /locus_tag="PLL14_02400"
     CDS             5537..6370
                     /gene="motD"
                     /locus_tag="PLL14_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HOG02800.1"
                     /translation="MARRQREEEKDNHERWLVSYADFITLLFAFFVVMYALSSVNEGK
                     YRILSNSMVSAFRNVPVNSSGTAPMVVPTSAPAVPKVTLTSKTQDTVRQQQREKMRHV
                     AKEILEVMAPLVEQGKVRVLETSRGVSIEINDSILFSPGQALLQPPLVKAMQSVAEVL
                     VASDFPITIEGHTDDLPIKNTQFPSNWELSAVRATTVLRLFADAGVAAARLTAIGYAD
                     TRPVEPNVLADGRARNRRVTILIDSEIPEKGTEVNLQGQAAQLKPATTVPAEALPPSS
                     R"
     gene            complement(6375..>6630)
                     /locus_tag="PLL14_02405"
     CDS             complement(6375..>6630)
                     /locus_tag="PLL14_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOG02801.1"
                     /translation="RSGVTAWLADHPDESDAGTAWTLLLSLATQARELNRVNGGLIQL
                     RMHHNAQALEVLLGGNGALALYGPDGQNAAPSARRISDRA"
BASE COUNT         1232 a         1912 c         2155 g         1331 t
ORIGIN      
        1 ccggacgggt tgccgaggtg gctgcacgct tcaccctcga cgccatgccg ggcaaacaga
       61 tggccattga cgccgacctc aatgccggcc tgatcggtga ggatgaggca cgcaagcggc
      121 gggcgacaat cgctcaggaa gcggatttct ttggttccat ggacggtgcg tcgaagttcg
      181 ttcgtggtga cgcggttgcc ggcattctga tcatgttgat caacgtcgtg ggcggacttt
      241 tcgtcggtgt cctgcagcac aacctggata tcggtacggc ggccaagacc tacacgctgc
      301 tgaccatcgg tgacgggctg gtggcccaga ttccggcgct gatcatctcg attgccgcgg
      361 gcatggtcgt gacgcgggtt ggcgacgatg aagatgtttc gcagcagttc atctcgcaac
      421 tgttcagcaa tcccaagctg ctgtttctga ccgcggcaat catcggtatg ctggggctca
      481 ttccaggcat gccaaatttc gtcttcctgc tcctggcaag cgctttgggg gccatggcat
      541 ggcgactgga aaagcgttcg cagctcgccg tgccagtgcc ggtgaccgcg gcacccgtcg
      601 cgccacggga aactcaggaa gccagctggg ccgacgtctc gccgctggac gtgctggcgc
      661 tggaggttgg gtatcggctg attcctcttg tcgacaagtc tcaggatggc gagttgttac
      721 gccgaatcag aggaatacgg aaaaaatttg cgcaggaagt gggcttcctg gtgtccccgg
      781 ttcatattcg cgacaatctg gagttgaaac ccaacgccta caagattctg ctcaagggcg
      841 ttgaaatcgg cgacggcgag gcctttccag gaaacctgct ggcgatcaat ccgggccggg
      901 tggccggaca gatcccaggg acggttacca aggatccggc cttcgggctc ccggcggtgt
      961 ggattgatcc agcactccgt gaacaggcgc aggcctacgg ttacaccgtc gtcgatgcca
     1021 gcaccgtggc ggcgacgcac ctcaaccacc tgattctctc gcacgcagcc gagctccttg
     1081 ggcggcaaga aacgcaggcc ttgctcgatc acctggccaa ggagatgccc aagctggtcg
     1141 aggatgtggt acccaaggtc ttgtcgcttg gtgtggtaca gaaagtgctt cgcaacctcc
     1201 tggaagaggg ggttcatctg cgcgacatgc ggacggtcat cgaaaccctg gccgatgtcg
     1261 ctccccgctc ccaggatcct gaagttctga cggcgcaggt tcggaccgcc ctgggtcgtt
     1321 cgatcgttca ggaactctat cccggaggtg ccgaaatgca ggtgatgtcg ctggatccgc
     1381 aactggagcg cctcctgctc caggccgttg ctggcggtgg cgatagcgcc ggcatcgagc
     1441 cggggctcgc cgaaacgctg gtgcgcgaag cggttgccgc ggcgcagcgc caggaggaag
     1501 ttggtctgcc gaccgtgctg ctggttcccg gcaatctccg gacgctgttg tcgcgcttcc
     1561 tccgtcgcag catttcccag ctgaaggtcc tggcacatgc ggaagtgcct gaaaacagga
     1621 tcatcaaagt cacttccatc attggacagc gcgtatgaac gtcagaaaat tcatcgcggc
     1681 gaccgcccga gatgccttgc gcaaggtcaa ggagactctc gggccggacg ccatcatcct
     1741 gtctaatcgg ggtattcccg gtggcgtgga gatcatggcc gtcgctgcac gtgacatgga
     1801 tatgatcgtg ccgacgtcgg agcgcgaagc gtcgcctgcc cgcgcgatgg actacccgac
     1861 agtctcgtcg gtcgaccgag gtcctgcaac cgcgcctgag ccgccgtcgg ttcggggggc
     1921 gtcgcctgcc gccggtcagc aaagtgctgc cgcacgctcc ttgccgccgc cactggcgag
     1981 gcctctggca ccaccgttgg agcgacccca gtcatcacga tcactgccgc cgctggagcg
     2041 gccgcgtccg caagccgaga gcatggccaa gggcgacgcg gaagtggttc cagcagcggt
     2101 gatggacgag atccgatcct tgcgaagaat cgtcgaacaa cagctcgccg gttttgcgtg
     2161 gggagaatcg gcgcgttccg aaccggtcaa gacagagatc cttcgtcaga tgctcgatgc
     2221 cggtttttcg ccgcactttg cacgtggcct gctcgctgac ctgccccgag acctggatag
     2281 tgtccaggcg ctggcgtggg tcaggggggg cgccgagcgc agcatgttga cgatcagtag
     2341 cgagaccgac attgtccacc gggggggcat ctatgcactg gtcgggccga ccggcgtcgg
     2401 caagacgacc accaccgcca agttggctgc acgctgcgtc ctgcggcacg gcgcaaggaa
     2461 actggcattg gtcaccactg atggttaccg gatcggtgcc cacgaacagt tgcgcatcta
     2521 tggccggatt ctcggagtat cggtgcatct tgcccgagac gccaaggatt tgcgagcgac
     2581 tttgcgtgac ttgcagcaca cccatatggt attgatcgac accatgggca tgagccagcg
     2641 cgaccggatg gtcagcgaac aggtcgccat gtttggtgac tgcaacgtga agtcgctgtt
     2701 gttgctgtcg gctaccagtc gcggtgacac gctggatgat gtagtcaggg cgtacagcgg
     2761 gctggacctt gcgggttgta tcttgaccaa ggtcgacgaa gcggcgagtc tggcctcgtc
     2821 gctcgacgtg atcattcgcc acggcttgct catgtattat gtgtcgaatg ggcaacgggt
     2881 accggaagac ctgcatttgc cgaatcgtcc ttacctgttg caccgcgctt tcaaggatct
     2941 gcccgagact tcgccgcatc gcctggccgg cgtcgagcct ggcttgatga tggccagctc
     3001 gaccgtcagc agtttcactg ccggaggcca gcgtgcctga gtttcgtggg gatcaggcgg
     3061 ctggtctacg gcgcctcctg gggcggcaga gtctgcgtat cgtgaccttt gctgccggta
     3121 gcatcggtgt cggcaaaagc gtggcggtgg ccaatctggc ggcctcgctg gcccgtcagg
     3181 gcagggaagt cctggtggtc gacgaaaaca ccgaagacgg catcgcggcg tattacggcg
     3241 tgttggcgcg ctatgatcta cagcaggtca tcgatcggga gaagtctctc tcggaggtga
     3301 tcctctccgt ggcaccaggc atccgcgtgt tgccggtagc acgggtggtg aggcacctcg
     3361 ccaggctcag cgatgccgag caacggatcc tgctcggttg tctcaccgag atcgggccgc
     3421 cggccgatgt ccttctggtc gacacttcac ttgatcaccc gctcggtttt tcgccactcg
     3481 gtcttgctgc gcatgagacg gtgatcgtcg tctcgcccag tagtgcggca attaccgagg
     3541 cgtacgcgct gatcaagaaa gtcagtctgg gttacgcgcg gcaggatttc cgcatcctgg
     3601 tcaacaaggc gcgggcagcc cgtgaagcgc gcgcgatata tgacaatatt gcgcacgtca
     3661 caggtagccg cgggctcgct cgcctcgact atgcgggcta tgtgccggtc gacgagcacc
     3721 tgcggcaggc ctccaggttg tgccagccgg tgagcgcact gtttccggac tctcctgccg
     3781 ccaaggcgta tcaaattgtg gccaacgact tgttgaattg gccgggagcc gatgacgaga
     3841 tcggcgggct cgaacatttc gtgcaacaat tgctactctt gagccaacgc atagaaccga
     3901 ttgctattta cgcctgatag cgcttccacg acgcatgtat aacgctgctg gtcaactcaa
     3961 caaggatcag ctcgtccagc gctttgctcc gctggtgagg cgcattgcct gtcatctgat
     4021 ggctcgcctg ccggccagtg tgcagttgga cgatctggtt cagaacggca tgctcggcct
     4081 gcttgatgcg attggccgtt tcgaggccgg catgggggca cagttcgaag tctacgcggc
     4141 acagcgcgtg cgtggcgcga tgctcgacgg gctacgcgag aacgactggt tgccgcgcag
     4201 cttgcggcac aactgtcggc ggatcgaagt tgccattgca caactcgaac aggaaaatgg
     4261 gcgtgcgccg tcggaaaagg aactcgccga tgctctgggg atgggcctgg ccgactacca
     4321 gaaaatgctg caggatgccc gtggccatca actggtatac ctggaagacc tggttaccga
     4381 acgcggtgac gattttctgg aacggcatct ggcagacgat gcggccgatc cggcgaagct
     4441 ttttgaggag ggaaacctgc gccaactgct ggtccaggcg attgaactgc tgccggagcg
     4501 ggagagactg atgatggccc tctactacga gcaggacctc aatctgcgcg agatcggcgc
     4561 ggtcctgggg gtaacggagt ctcgtgcctg ccagttgcac agtcaggcgg tgttgcgctt
     4621 gcgcgcccgc ctgtttggtg ggaacgtgac caagacgacg aaagtggcac ggcatggata
     4681 ggatcagcgt cttcgggctg ctgatcggtc tgttggcgat catcggcggc cagatcctgg
     4741 agggtggcca tatcggttcc ttgtcgcaac ccacggcgat gctgattgtg gtcggtggta
     4801 cgcttggggc cgtcatgttg cagagtccct acgctacttt tgtgcgtggc atccgtatgg
     4861 tgcggtggat ctggtacccg ccggtggtca atcatctgca gttgatccag caggtctcag
     4921 gctggagcca ggtgtcgcgg cgggaaggct tgctggcact ggatcccatt gtcaacgaat
     4981 tgaaggacga gtttacgcgc aaggggctgc aactggtggt cgacggggcc gagcccgacc
     5041 ggctgcggga agtgatggag gtcgagatca gcacctacga acaggaaatg aagttgtcgg
     5101 cgcggatctg ggaggccgcc ggcggctact cgccaaccat cgggattctt ggcgcggtga
     5161 tgggtttgat tcaagtgatg gagaatttgt ccgatccatc caaactcggg gccgggattg
     5221 ccgtggcgtt cgttgccacg atctatggcg ttggcctggc caatctggtt ttcctgccga
     5281 tggccaacaa actgaaagcg catatcaacc gcctcatcgt gcagagagaa atgattgtcg
     5341 acgggctggt gggtatcgcc aacggcgata atccccgtat catcgaaagc cgtctgcgcg
     5401 gctacatctt ctgacgccag tgaagattcc aggtacgccc aatcctgtcc accatgatcc
     5461 ctgtcggcag tccttgcttc caactcgtgg ggggagggcg atgacccgct ggccgaagca
     5521 acggtccaac tgagcgatgg cacgtcggca gcgcgaagaa gagaaggaca atcacgagcg
     5581 ctggctcgtg tcgtacgcgg atttcatcac cctgctgttc gccttttttg tggtgatgta
     5641 cgcgctctcc tctgtgaacg aaggaaagta tcgcattctg agcaactcga tggtcagcgc
     5701 ttttcgcaat gtccccgtca acagcagcgg aaccgcgcca atggtcgtgc ctacctcggc
     5761 gccggccgtg ccgaaggtca ccctgacgag caagacgcag gacactgtga gacagcagca
     5821 gcgcgaaaaa atgcggcatg ttgcgaagga gatccttgaa gtgatggcgc cgctggttga
     5881 gcaggggaag gttcgcgtgc tcgaaaccag ccgcggtgta agcatagaaa tcaacgacag
     5941 catcctgttt tcgccagggc aggccctgct gcagccgcca ttggtaaaag ccatgcagtc
     6001 tgtggcggag gtcttggtcg cctcggattt cccgatcacg atcgagggac acaccgatga
     6061 cctcccgatc aagaatactc aatttccctc aaactgggag ctttctgcag tgcgtgcgac
     6121 caccgtcctg cgcttgttcg ccgatgcagg cgtagcggca gcgcgcttga ctgcgattgg
     6181 ctacgccgac acccgtccgg tggagcccaa tgtgcttgcg gatggacggg ctcgcaatcg
     6241 tcgggtgacg attctgattg attcggaaat tcccgaaaag ggcacggagg tgaatctgca
     6301 ggggcaagcg gcgcaactca agccagcgac gacggttccc gctgaggcgc tgccgccatc
     6361 gagtcgctag cgggctaggc gcggtcgctg atccgccgag cgctcggtgc cgcgttctgt
     6421 ccatccgggc catagagcgc gagggcaccg ttgccgccca ggagtacttc aagggcctgc
     6481 gcgttgtggt gcatgcgcaa ctggatgagg ccgccattga cacggttcag ttcacgtgcc
     6541 tgggtggcga gcgacaacag caaggtccac gcggtgccag catcgctctc gtcggggtgg
     6601 tctgccaacc aggcggttac accggagcgg
//
LOCUS       DAOSIT010000055         6601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10572_length_6601_cov_8.849068, whole genome shotgun sequence.
ACCESSION   DAOSIT010000055 DAOSIT010000000
VERSION     DAOSIT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10572_length_6601_cov_8.849068"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1936)
                     /gene="recQ"
                     /locus_tag="PLL14_02410"
     CDS             complement(125..1936)
                     /gene="recQ"
                     /locus_tag="PLL14_02410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOG02802.1"
                     /translation="MTSPAVDILRRVFGYASFRGQQAEIVDQVVRGGDALVLMPTGGG
                     KSLCYQIPALVRPGVGVVVSPLIALMQDQVDALRLAGVRAAFLNSTLDFQSLVDTERC
                     LLRGELDLIYVAPERLLTDRFIVLLEQLVGRQQLALFAIDEAHCVSQWGHDFRPEYIQ
                     LSQLHERFPSVPRIALTATADQLTRQEIITRLSLAEARLFVASFDRPNIRYTVVERDN
                     PRKQLLFFLSAHRGEAGIVYCLSRRKVDETAVWLNAQGVAALPYHAGLTAAERQHHQQ
                     RFLREDGLVMVATIAFGMGIDKPDVRFVVHLDLPKSLEAYYQETGRAGRDGDPSEAWM
                     TYGLNDVVIHRQMIDESSAPPEQKRIERQKLDSMLAYCESAQCRRVLLLNYFGEEAAP
                     CGNCDVCLDPPLLWDGTIAAQKALSAALRTGQRFGAGHLIDILRGKSTDKVKQFGHER
                     LPTFGVGAEMDDLGWRSVFRQLLAGGILEADATAYGALKLTGDARPILKGEASIKMRR
                     RLETTKGRQARNRVTPDRPRQADDSPLLARLRAWRSEKAREQGVPAYVILHDRALHEI
                     AALLPDSVPALLAVPGIGVAKAERYGRELLTLVAASA"
     gene            complement(2046..2777)
                     /locus_tag="PLL14_02415"
     CDS             complement(2046..2777)
                     /locus_tag="PLL14_02415"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOG02803.1"
                     /translation="MILFISDLHLAPQAPGVARLFLDFLGGRARAAEHLFILGDLFEA
                     WPGDDCLDDARDPMAAEIVAALRLLVTAGIGVSVMHGNRDFLLGEVFAARSGARLLPD
                     PHVLSLPTWQFVLSHGDALCTDDHEYQSFRSLVRRAEWQAAFLARPLAERRAVAAVLR
                     QKSESAKRGKAEYLMDVNPGTTDDFLRAHGYASLIHGHTHRPATHDHIVDGIHVERWV
                     LADWTETQGECLCWNGSNLARERLR"
     gene            complement(2809..3300)
                     /locus_tag="PLL14_02420"
     CDS             complement(2809..3300)
                     /locus_tag="PLL14_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG02804.1"
                     /translation="MIKLHTNFGIIGIEVDAEKAPLSSANFLAYVAAGHYDNTIFHRV
                     IPGFMIQGGGFMPGMKQKACQAPIRNEADNGLKNIAYTVAMARTAEPHSATAQFFINV
                     VNNDFLNHRAPNAQGWGYCVFGKVVEGTEVVDRIKAVKTATAGGHSDVPVEDVLIERA
                     EVC"
     gene            complement(3430..3990)
                     /locus_tag="PLL14_02425"
     CDS             complement(3430..3990)
                     /locus_tag="PLL14_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG02805.1"
                     /translation="MHRKLALIIGGLLSFAALAAPSVEMQTSMGRIVIELDSEKAPKT
                     VQNFVQYVNEGFYNGTVFHRVIPGFMIQGGGFTVDLEQKAAPRKVDNEGKNGLKNDRG
                     TIAMARTADPNSASSQFFINHRDNASLNYPGSDGWGYAVFGKVTQGLDVVDRIAKVAT
                     GNRSMHQNVPLEPVTIQSVRVIPEKK"
     gene            complement(4125..5219)
                     /locus_tag="PLL14_02430"
     CDS             complement(4125..5219)
                     /locus_tag="PLL14_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG02806.1"
                     /translation="MRPNLPTLPALLHALLLALVLICADSPAIADSLADAQRLLKQGL
                     SSQALEKVDTYLAGKPKEAQGQFLKGLILAEMGRPAEAIAVFSKLTEDYPELPEPYNN
                     LAVLYARQKQYDKARTALEMAIRTYPGYATAYENLGDVHANLASQAYDKALQLDPAKK
                     IAQTKLFRLRELISTAGGAGGKQVAAIGEVGEVGATRGTDTTLTVGATTTPAARTTDK
                     PAPLRAAETLAERPVPKAVDAVAALPRNPVAATGADEAAVIRALQAWANAWSRKDAKA
                     YLAYYAADFLPPRGMSRKAWEIERMRPMQKPGKLQVEIEDVKVSFADDKATARFRQHY
                     ASPYLKSSVGKTMVLVRVRGRWLIQQERVG"
     gene            5361..>6601
                     /gene="cysS"
                     /locus_tag="PLL14_02435"
     CDS             5361..>6601
                     /gene="cysS"
                     /locus_tag="PLL14_02435"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG02807.1"
                     /translation="MLKIYNSLAREKQNFVPITPGSVRMYVCGMTVYDYCHLGHARVL
                     VVFDMVQRWLRASGLQVNYVRNITDIDDKIIKRAVDNKESISELTERFIRCMDEDAAA
                     LGVEKPDHEPRATAYIPQMLELIRRLEANGLAYQTAAGDMNFSVRKFPGYGKLSGKSV
                     DDLRAGERVDVDGTKDDPLDFVLWKHAKPGEPCWASPWGNGRPGWHIECSAMGSDLLG
                     KHFDIHGGGQDLQFPHHENEIAQSEGAHRCRFVNYWMHNGFVRVDDEKMSKSLGNFFT
                     IREILKKYDPEVLRFFILRAHYRSPLNYSEGHLDDARQALSRLYTALKTVPAEVVPID
                     WNQSPALRFRQAMDDDFNTPEACAVLFELAGEVNRSQSPVLAGQLRALAGLLGLLTRD
                     PQVFLQATPRNEGEISAADIEA"
BASE COUNT         1314 a         2077 c         1950 g         1260 t
ORIGIN      
        1 ctcactcctg tggtctgcgc catgcccgcg aactccgccg cgaacacgta cgcattgtcc
       61 agccctcggg gcagagtgtc gcgctcgacg ctctccaccc ttacccggtc cgagccacct
      121 gaactcaggc gctggcggca acgagggtca gcaattcccg gccatagcgt tcggccttgg
      181 cgacgccgat ccccgggacg gcaagcagcg ctggtacgga atccgggagc agtgcggcga
      241 tctcgtgcag tgcccgatcg tgaaggatca cgtaagcggg cacaccctgc tcacgggcct
      301 tctcgctgcg ccaggcgcgc aatctggcca gtagcggtga atcgtcggct tgccgggggc
      361 ggtcgggagt gaccctgttc ctggcttgcc tgccctttgt cgtttccagc cgccgccgca
      421 tcttgatgct ggcttctccc ttgagaatcg gccgcgcgtc gccggtcagc ttcaaggccc
      481 cataggctgt ggcgtcggct tcgaggatgc cgcccgcaag caactggcga aataccgaac
      541 gccaacccag gtcatccatt tccgcgccaa cgccgaaggt gggcagacgt tcatggccaa
      601 actgcttcac cttgtccgta gacttgccgc gcaggatgtc tatcagatgc ccggcgccaa
      661 agcgctgacc ggtgcgcaag gctgccgaca gcgccttctg cgccgcgatg gtgccatccc
      721 acagcaacgg cggatcgaga cagacatcgc aattgccgca aggtgccgct tcttcgccaa
      781 aatagttcag caggagtacg cgccggcact gcgccgattc gcaataggcc agcatgctgt
      841 cgagcttctg ccgctcgatg cgtttctgct cgggtggcgc cgacgactcg tcgatcatct
      901 gccgatggat caccacatcg ttgagcccgt aagtcatcca ggcttcggac gggtcgccgt
      961 cacgccccgc ccgcccggtt tcctgataat acgcttcgag gctcttgggt agatcgaggt
     1021 gcaccacgaa gcgtacgtcc ggcttgtcga tccccatccc gaaggcaatc gttgccacca
     1081 tcaccaggcc gtcctcgcgg aggaagcgct gctggtggtg ttggcgttcg gcggccgtca
     1141 acccggcatg gtagggcaag gcagccactc cctgtgcatt cagccacacc gccgtttcat
     1201 cgaccttgcg gcgcgacagg caataaacga ttccggcttc cccccgatgg gcactcagga
     1261 agaagagcag ttgcttgcgc gggttgtcgc gctcaacgac cgtatagcgg atgtttggcc
     1321 ggtcgaaact cgccacgaac agcctcgcct cggcaagact caggcgggtg atgatttcct
     1381 gtcgtgtcag ctggtcggca gtcgccgtca gcgcaatacg cggcacggaa ggaaagcgct
     1441 cgtgcaattg cgagagctgg atatactccg ggcgaaagtc gtgcccccat tgcgaaacac
     1501 agtgggcctc gtcaatcgcg aacagcgcga gctgctggcg tcccacaagc tgttcgagca
     1561 agacgataaa acggtcggta agcaaacgtt cgggagcgac gtagatcaga tccagttcgc
     1621 cacgcagcaa acaacgctcg gtgtcgacca gcgactggaa atccagcgtc gaattcagga
     1681 acgctgcgcg cacacccgcc agccgcaagg cgtcgacctg gtcctgcatc agcgcaatca
     1741 gcggcgagac gaccacccca acgcccggtc ggacgagtgc gggaatctgg taacagagcg
     1801 acttgccgcc accggtcggc atcagcacca gagcatcccc tcctctgacg acctggtcga
     1861 cgatctccgc ctgctgacca cgaaaggacg catagccaaa gacgcggcgg aggatgtcga
     1921 cggcgggaga agtcattgcc ggagtggtaa cgacgatgtc aagccctcag gcataggaca
     1981 gaagggacag ccgccagcgt agcgccgtat tgccggcgct gaccggtgac aaggtctgaa
     2041 atcagtcagc gcaagcgctc ccgggcaaga ttcgagccgt tccagcacag gcactcgccc
     2101 tgtgtctcgg tccagtcggc gagtacccaa cgctcgacgt ggatgccgtc gacgatatgg
     2161 tcgtgggtcg ccggacgatg cgtgtggccg tggatcaggc ttgcatagcc gtgcgcgcgc
     2221 agaaaatcgt cggtggtacc gggattgacg tccatcagat actccgcctt gccacgcttg
     2281 gccgactcgc ttttctggcg cagcaccgcc gccactgccc gacgttctgc cagtggcctg
     2341 gcgagaaagg cggcttgcca ctccgcgcgg cgaacgagcg aacgaaacga ctgatattcg
     2401 tgatcgtccg tacacaacgc gtcaccatgc gacagcacga actgccaggt gggcaacgac
     2461 aggacatgcg ggtccggcag cagccgcgcg ccgctccggg ccgcaaaaac ctcaccgagc
     2521 agaaagtcgc gattgccatg catcaccgaa acgccaatgc cagccgtgac cagcaaacgc
     2581 agtgcggcga caatctccgc cgccatcgga tcccgcgcat cgtcaaggca atcgtcgccc
     2641 ggccaggctt cgaaaagatc gccaagaatg aacaggtgct cggccgcacg cgcccgccca
     2701 ccgagaaaat caagaaagag tcgcgcgacc cccggcgctt gcggcgccag atgcaggtcg
     2761 gaaatgaaca ggatcaagga ggctaccgat cggtcaccgg cactcgcctc agcaaacttc
     2821 ggcgcgctcg atcagcacgt cctcgacggg cacgtcgctg tggccaccgg cagtggctgt
     2881 ttttacggcc ttgatgcggt cgacgacctc agtaccctcc acgaccttgc cgaaaacaca
     2941 gtagccccaa ccttgcgcat tgggcgctcg gtggttcagg aagtcattgt tcaccacgtt
     3001 gatgaaaaac tgcgcggtgg ccgaatgcgg ttcagcggtg cgtgccatgg caaccgtgta
     3061 agcgatgttc ttcagtccat tgtcggcctc attcctgatc ggtgcctgac atgccttctg
     3121 cttcatgccc ggcatgaagc cgccgccctg aatcatgaag ccgggaatca cacggtggaa
     3181 aatggtattg tcgtagtggc cggcggcgac ataggccagg aaatttgcgg acgacagggg
     3241 tgctttctcg gcatcgactt cgataccgat gatgccgaaa ttggtgtgta gcttgatcat
     3301 ggctgtccta gttgtgagag ttgcttctgc aacccgcgta atcccctcgc acgaaaatgt
     3361 ggaagtgggg atggagtgag ggatacggtc agcaccggca tgcgccgagt tctttacaac
     3421 ccgtaaccgc tatttctttt cgggaatgac cctgaccgac tggatggtta ccggctcaag
     3481 cggcacgttc tggtgcatgg agcggtttcc ggtggccacc ttggcaatcc tgtccaccac
     3541 atcgagtccc tgcgtgacct tgccaaaaac ggcgtagccc cagccgtcgg atcctgggta
     3601 gttgagcgat gcgttgtcac gatgattgat gaaaaactgc gaactcgccg aattcgggtc
     3661 cgccgtacgg gccatggcaa tcgtgccgcg gtcgttcttc agaccattct ttccttcgtt
     3721 atccactttc cggggcgccg ccttctgttc cagatcaacc gtaaaaccac ctccctggat
     3781 catgaaaccc ggaatgacgc ggtgaaatac cgtgccgttg tagaaccctt cattgacgta
     3841 ctgtacaaaa ttctggacag tttttggggc tttctcggaa tcgagttcga tcacaatccg
     3901 gcccatgctg gtctgcattt cgacgcttgg cgcagccaaa gcggcgaagg acagcaatcc
     3961 accgataatc aaggcgagtt tcctgtgcat cacaccattc cttcccaagt gatttttcag
     4021 gtgccgccga cgcctgccgg tcgcgcactc gtttcccagc gccggacagc taccgccggg
     4081 cggtgtgccg tcctctgagc gtccgcacgc ctctgtgctg ctcatcaacc cacgcgttcc
     4141 tgctggatca accatcgccc cctgaccctg acgagaacca tggtcttgcc gacagaagat
     4201 ttcagatagg gggaagcata gtgctgcctg aaccgggctg tcgccttgtc gtcggcaaaa
     4261 gacaccttga catcctcgat ttcgacctgc aatttgcccg gtttctgcat cggccgcata
     4321 cgctcgattt cccatgcctt gcgtgacatc cctcgtggcg gcaggaaatc ggcggcgtaa
     4381 taagcaaggt aagccttcgc gtccttgcgt gaccaggcat tcgcccaggc ttgtaacgcc
     4441 cggatcaccg ccgcctcgtc agcgccggta gcggccacag gattcctggg cagcgctgct
     4501 acggcgtcca ccgctttcgg caccggccgc tcggccagcg tctccgccgc acggagcggt
     4561 gcaggcttgt ctgtcgttcg ggccgcaggc gttgtcgtag cgccgacagt cagcgtggta
     4621 tccgtcccac gcgtggcccc gacctcgccc acctcgccga tcgcggcgac ttgcttgccc
     4681 cccgccccac ccgcagtgct gatcagttca cgcagccgga acaacttggt ctgtgcgatt
     4741 ttctttgctg gatcgagttg caaagccttg tcataagcct ggctggcgag attggcatgc
     4801 acatcgccaa gattctcgta ggcagtagcg tagccaggat aggttctgat cgccatctcg
     4861 agcgccgtac gcgccttgtc gtactgtttc tgccgggcat agagcacagc gaggttgtta
     4921 tacggttcgg gaagttcggg atagtcttcg gtcagcttgc tgaacacggc aatcgcttca
     4981 gcaggacgcc ccatctcggc gagaatcagg cccttgagaa attgcccctg cgcctccttc
     5041 ggcttgccgg ccagataagt gtcgactttc tcgagggcct gcgacgacag cccctgtttc
     5101 aacaggcgct gcgcatcggc caggctgtct gcaatggccg gggagtctgc gcaaatcaaa
     5161 accagggcaa ggagcaaagc gtgcaagagc gccggcaagg tcgggaggtt cgggcgcatc
     5221 gttatggtat acttggtctg gcaacggttg gtagtggttg gcgactcgac cgatcgattc
     5281 taacaaaagc cgcgaccaaa ccaaagcact gtacgccgcc agcgccacgc tttccgcggc
     5341 tcattgccgt ggacccgaaa atgctgaaaa tctacaactc gctggccaga gaaaagcaga
     5401 acttcgtacc catcaccccg ggcagcgtcc gcatgtatgt ctgcgggatg accgtttacg
     5461 attactgcca tcttggtcat gcccgcgttc tggtggtgtt cgacatggtg cagcgctggc
     5521 tgcgcgcgag tggactgcag gtcaactacg tccggaacat taccgatatt gacgacaaga
     5581 tcatcaagcg cgctgtcgac aacaaggaaa gcatcagcga actgacggag cgcttcatcc
     5641 gctgcatgga tgaggacgcc gccgccttgg gagtggaaaa accggatcat gaaccgcgtg
     5701 cgactgcgta catcccgcag atgctcgaat tgatccggcg actggaggcg aacggactgg
     5761 cttaccagac ggcggccggc gacatgaact tttcggtacg caagtttccg ggctatggaa
     5821 agctctcggg caagtcggtt gacgatctgc gggccggcga acgcgttgac gtcgacggga
     5881 ccaaggacga cccactcgac ttcgtcctgt ggaaacacgc gaaacctggc gagccttgct
     5941 gggcttcgcc ttggggcaat ggccgaccgg gctggcacat cgaatgctcg gcgatgggtt
     6001 cggacctgct cggcaagcac ttcgacatcc acggcggtgg ccaggacctg cagtttcccc
     6061 accacgaaaa tgaaatcgcc cagtccgagg gcgcacatcg ctgccgcttc gtcaactact
     6121 ggatgcacaa cggttttgtc cgcgtcgatg acgaaaagat gtcgaaatcg ctcggcaact
     6181 ttttcacgat cagggagatt ctgaagaaat acgatcccga ggtgctgcgc ttcttcatcc
     6241 ttcgtgccca ctaccgcagc cctctgaact attccgaagg ccatctcgac gatgcccggc
     6301 aggcgctgag ccggctctac actgcgctca agacagtgcc cgcggaagtc gtgccgatcg
     6361 actggaatca gtcaccagca cttcgcttcc ggcaggccat ggacgacgac ttcaacacac
     6421 ccgaagcctg cgccgtgctg ttcgaactgg ctggtgaggt caaccgcagc cagtctccgg
     6481 tcctcgcggg gcaattgcgg gcgctggcgg gtctgctggg tctcctgacg cgagacccgc
     6541 aggtcttcct gcaagcgacg ccgcggaacg aaggcgaaat ttccgccgca gacatcgaag
     6601 c
//
LOCUS       DAOSIT010000056         6492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_10806_length_6492_cov_11.800062, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000056 DAOSIT010000000
VERSION     DAOSIT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10806_length_6492_cov_11.800062"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..>2488
                     /locus_tag="PLL14_02440"
     CDS             45..>2488
                     /locus_tag="PLL14_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG02808.1"
                     /translation="MSAPTDFDIGPLTWVKSEIDLALERGEKALQSMTANAEDTHGDH
                     TQIRFCRTHLHQVQGALTIVGLDGVTQFAEALESMLEAIERDERPATPEVIELAQRAM
                     AALGHYLADLIGGQPNQPLRLLPLYREVQAARGQERVLATDLFFPDLRVRPPQRAERT
                     AKIGRSELQARLRQQRAHFQRGLLGWLRAPQSGAGVPEMLDAVKRIESLQDAPSARAF
                     WWIATAFLTALGERALPADGDARQLCSRIDLQIRRLLEGSSNVAERLVRDALYLVSNA
                     QSSSRAVLQVKDAYHLADLLPGPASATPIAMESVRRQVREAIAATEETWNKFCAGTMQ
                     VLPVFRANTTTLTTLVDELGHADFRRLVHAISEVAGLLTHSPSRHSEALAMETATAIL
                     LAQHAQENMQYLGSSFSHQVDVMVARLHDCIAGTPPKPESELPSLDEMSRQAQEKMLV
                     GQVAREIQNNLAQIEQVLDGFFRDAEKSADLGSLETPLRQVIGALAMMRHDGAVTVLR
                     QCATAIESFAQPAYVPHEADFVGVAEQLSLIGFFVDAMQHGAADFESFVSQMQSDAAR
                     KSVDEEPAATVEQELEQQKRETHALLVALKEQPGDAGLRQEVQQNLTALQKDADLVAD
                     KALGQQTKHVLTALAAGGDAARQIDAAMATLKPQAPEAPPPSAQTLQLSQASTEEVDA
                     ELLGIFLEEADEVLASVDGNLRALKDKPHDVELLTAIRRSAHTLKGSGRMVGLKDLGD
                     AAWAVEQVLNLWLRQEQAVTPPLLDLLDQAYAVFLAWMTHLKTGQGHAPDPRSMIATA
                     ASLRDGSELPIGVPPG"
     assembly_gap    2489..2588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2589..4904
                     /locus_tag="PLL14_02445"
     CDS             <2589..4904
                     /locus_tag="PLL14_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG02809.1"
                     /translation="LPELHDELDEQLLPLFIEEALDLNQNIAAQLHAWRNNPADGEPV
                     RRLARLFHTLKGSARMAGAMNLGEMTHAIETRMSDAQDAGTAPLDLIDDIDNAFDVIV
                     QIVEHLQRGESSEAPVDIPDEAASQLPTAAQPTSDLAAAGDQGSVAVTAETPDSESDA
                     AVQRAMLRVRADLIDRLVNEAGELSIARSRIEGEMRAIKASLLDLTENVIRLRRQLRD
                     IELQAELQMQSRAAQTGEHHADFDPLEFDRFTRFQELTRMMAESVNDVATVQQNLLKN
                     LDDANAAIVAQARLNREVQQELMSVRMVPFASIADRLYRIVRQAGKEVGKRANLEISG
                     AQLELDRTVLDKMLAPLEHMLRNAVAHGLEDAPLRLAKGKPEIGEISLKLAQEGNEII
                     LTFSDDGTGLDLDRLRERGLRAGLLSESEAADTARVVDLIFAPGISTASEVSRLSGRG
                     IGMDVLKSEVTSLGGRIEVLSAPDQGTTFRLYLPLTLAVTKALLVRSGNREYAIPAAM
                     IEQVLDLKEKGLTRIREARAASWTGNLYPFSYLPHLLGETRALPEKHQQYWVLLLRSG
                     TQRVALQVDELLGNQEIVVKNIGPQLARVIGVDGATVLGNGQVVLILNPIALASRDRA
                     GPAVSPVPTVRQPTAADLVTATLPTVMIVDDSLTVRKISSRLLAREGYQVMTAKDGVD
                     ALEQLVAVVPDVMLVDIEMPRMDGFELTRNVRADARLQKVPIIIITSRTADKHRQYAL
                     ELGVNHYLGKPYQEDELLRLVAGHVRAQRQS"
     gene            complement(4882..6330)
                     /locus_tag="PLL14_02450"
     CDS             complement(4882..6330)
                     /locus_tag="PLL14_02450"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOG02810.1"
                     /translation="MEAILVWFRRDLRDYDHAALSEALRRGRQVYCAFVFDREILDTL
                     ERRSDRRVEFIRESLVELDAALRVRGGALIVRHGWVTREIPELARSLGVAAVFANRDY
                     EPQAKRRDSAVAALLQADSIAFESFKDQALFDADEVLTQAGRPYTVFTPYRNAWLRRL
                     GEDDWQPHAGRGRGLAVAPVGGGVPTLAELGFAASDLRALGIAPGMSGGRQRLSDFLG
                     RMARYREQRDFPAVKGVSYLSVHLRFGTLSVRELVAQAIAAGALHAPTGGAATWLSEL
                     VWRDFYFMILDRFPYVSERSFKPPYDAIVWEQGPHADAAFAAWCAGRTGYPLVDAAMR
                     QLNSTGYMHNRLRMLVASFLAKDLGIDWRRGESYFASQLNDFDLSANNGGWQWAASSG
                     CDAQPYFRIFNPVTQSERFDPAGRFIRRYLPELASVPNQWVHAPWRMSEPEQAACGVR
                     IGRDTPAPLVDHAQARHRTLQRYAVVKTGAAP"
BASE COUNT         1277 a         2085 c         2009 g         1021 t
ORIGIN      
        1 ctgagccctc gtgccaagag actggactgg agagactgcc ccggatgagt gccccgacgg
       61 acttcgatat cggcccgctg acctgggtca agagcgagat cgacctcgcc ctggagcgtg
      121 gcgaaaaggc gttgcagagc atgactgcga atgccgagga tacgcacggc gaccacacgc
      181 aaatccgctt ctgtcgcact catctgcacc aggtgcaagg cgccctgacg atcgtcggcc
      241 tggacggagt tacccagttt gccgaagcac tggagtcgat gctcgaggcc atcgagagag
      301 acgaacgccc agccacgccg gaggtcatcg aactcgcgca acgtgccatg gcggcgcttg
      361 ggcattatct cgccgatctg atcggcggcc agccgaacca gcccctgcgg ctattgccgc
      421 tgtaccgtga agtgcaggcc gcgcgcgggc aggagagggt cttggcgacg gatctcttct
      481 tccctgacct gcgcgtacga ccaccgcaac gagccgagcg gacggcgaaa atcggtcgca
      541 gcgaactgca ggcccgcctg cgtcagcaaa gagcccattt ccagcgcggt ctgctcggtt
      601 ggttgcgtgc cccccagtcc ggagccggag ttcccgagat gctcgacgcc gtcaagcgca
      661 tcgagtccct ccaggacgcc ccgtccgcac gcgccttctg gtggatcgcc accgctttcc
      721 tgaccgcgct gggtgagcgc gcgctgccgg cggacggcga tgccagacag ctctgcagcc
      781 gtatcgacct gcagatccgc cgcctcctcg aaggttcgag caatgtcgcc gagcgcctgg
      841 tgcgcgatgc cctgtatctg gtcagcaacg cccagagcag cagtcgcgcg gtgctccagg
      901 tcaaggatgc ctaccacctg gcagacttgc tgcccggacc ggcaagcgct actccgatcg
      961 ccatggagtc ggtccgccgc caggtgcgtg aagcgatcgc cgctacagaa gaaacctgga
     1021 acaagttctg cgccggaaca atgcaggttc tgccggtatt ccgggccaac accacgacgt
     1081 tgaccacgct ggtcgacgaa ctgggtcacg cagatttccg tcgcctcgtg catgcgatca
     1141 gcgaggtggc gggcctgctg acgcattctc catcgcgcca cagcgaagct ctggccatgg
     1201 aaacggcgac ggcgatcctg ctcgcgcagc acgcgcagga gaacatgcag tacctgggca
     1261 gcagtttctc tcaccaggtg gacgtgatgg tcgcccgcct gcacgactgc atcgcgggca
     1321 ccccgccgaa gccggaatcc gagcttccct cgctcgacga gatgtcgcgc caggcgcagg
     1381 aaaagatgct cgtcggccag gtcgccagag aaatccagaa caaccttgcg cagatcgagc
     1441 aggtccttga cggcttcttc cgtgacgccg aaaagagtgc cgatctgggc agcctggaaa
     1501 caccgctgcg acaggtcatc ggcgcgctgg ccatgatgcg ccatgacggc gcggtgacgg
     1561 ttctccgcca gtgcgcaaca gccatcgaaa gctttgcgca accggcctat gtcccgcacg
     1621 aggctgactt cgtaggcgtt gccgagcagt tgtcgctgat cggattcttt gtcgatgcga
     1681 tgcaacacgg agccgccgac ttcgagagtt tcgtcagcca gatgcagtcg gacgccgcca
     1741 gaaaatccgt cgacgaagaa ccggcggcga cggtcgaaca ggaactggag cagcagaagc
     1801 gcgaaacaca cgccttgctg gtggcgctca aggaacagcc tggcgatgcc ggtctgcgtc
     1861 aggaggttca acagaatctg acggcgctgc aaaaggacgc cgacctggtc gccgacaagg
     1921 cactcggaca acaaaccaag catgtactca ccgcgctcgc tgccggcggc gacgccgccc
     1981 ggcagatcga cgcggcgatg gcaacgctca aaccgcaagc acccgaggca ccgccgccct
     2041 cggcacagac cttgcagctc tcgcaggcct ccaccgaaga agtcgatgcc gaacttctcg
     2101 gaatctttct tgaggaagcc gatgaagtgc tggccagcgt cgatggcaac ctgcgggcac
     2161 tgaaggacaa accgcatgac gtcgaactgc tgaccgcaat ccgtcgttcg gcgcacaccc
     2221 tcaagggcag cggtcgaatg gtcgggctca aggatctcgg tgacgccgca tgggcggtcg
     2281 agcaggttct caacctctgg ttgcgtcagg agcaagcggt taccccgccg ctcctcgacc
     2341 tgctcgatca ggcgtatgcc gtatttctcg cctggatgac gcacctcaag accgggcagg
     2401 ggcacgcgcc cgatccacgc agcatgatcg cgaccgcagc gtcactgcgc gacggcagcg
     2461 agttgccgat cggcgtgcca ccgggcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnct cccggaactg cacgacgaac tggacgagca actgctgccc ctctttatcg
     2641 aggaagcgct cgacctcaac cagaacatcg cggcgcagtt gcatgcctgg cgaaacaatc
     2701 ccgccgatgg cgaacccgtc cgccgattgg cgcgcctgtt ccatacgctc aagggcagcg
     2761 ccaggatggc cggggcgatg aacctcggtg aaatgacgca cgccatcgaa acgcgcatga
     2821 gcgatgcgca ggatgccggt accgcgccgc tggacctgat tgacgacatc gacaatgctt
     2881 tcgacgtgat tgtgcagatt gtcgagcacc tgcagcgtgg ggaatcgtcc gaggcacccg
     2941 tggacatacc ggacgaagcg gcgtcgcaac tgcccacagc ggcccagccc acctccgatc
     3001 tggccgcggc tggcgatcag ggcagcgtgg ccgtcacggc cgagacgcca gacagcgaaa
     3061 gcgatgccgc cgtccagcgc gcgatgctgc gtgtgcgtgc cgacctgatc gaccggttgg
     3121 tcaacgaggc tggcgaactg tcgatcgccc gctcgcgtat cgaaggcgag atgcgagcga
     3181 tcaaggcgtc gctgctcgac ctgaccgaga atgtcatccg cctgcgtcgg caattgcgcg
     3241 acatcgaact gcaggccgaa ctgcagatgc agtcacgtgc ggcgcagacc ggtgagcacc
     3301 acgcggattt cgatccgctc gaattcgatc gcttcacgcg cttccaggaa ctgacgcgga
     3361 tgatggccga atcggtcaac gatgtggcca ccgtccagca gaatctgctg aaaaacctgg
     3421 acgacgccaa cgcggcgatc gtcgcgcagg cgcgcctgaa ccgtgaggtt cagcaggaac
     3481 tgatgtcggt acgcatggta ccgtttgcca gcattgccga ccgcctttac cgtatcgttc
     3541 gtcaggcggg caaggaagtc ggcaaacgag ccaatctgga aatcagtggt gcgcaacttg
     3601 aactcgacag gacggtgctc gacaagatgc tggcgccgct cgaacacatg ctgcgcaacg
     3661 ccgtcgcaca cggacttgaa gacgcgccgc tgcgcctggc caagggcaag ccggaaatcg
     3721 gcgagatctc gctgaaactg gcgcaggaag gcaacgagat catcctcacc ttctcggatg
     3781 acggcaccgg cctggacctt gaccgcttgc gcgagcgcgg tctgcgcgcc ggcctactga
     3841 gcgaaagcga agccgccgat acggcacgcg tggtggatct gatttttgcc ccgggaatct
     3901 cgacggccag tgaggtgtcg cgcctgtccg gtcgcggcat cgggatggat gtcctcaaga
     3961 gtgaggtgac cagcctgggt ggccgcattg aagtgctgtc cgcccccgat cagggaacga
     4021 cgttccggct ttatctgccg ctcacgctgg cggtgaccaa agccttgctg gtgcgctccg
     4081 gcaaccgcga gtacgcgatt cctgcggcga tgatcgagca ggtgctcgac ctcaaggaaa
     4141 agggactgac ccgaattcgc gaagcgcgtg ccgcatcctg gacggggaat ctgtatccct
     4201 tcagctacct gccgcatctc ctcggcgaaa cgcgtgcgct gccggagaaa catcagcagt
     4261 attgggtcct gctgctacgc agcggcaccc agcgggtcgc cctgcaagtc gacgaactgc
     4321 tgggcaacca ggaaatcgtg gtcaagaaca tcggaccaca actggcccgg gtcatcggtg
     4381 tcgatggcgc caccgttctc ggcaacggcc aggtggtgct gattctcaac ccgatcgcac
     4441 tggccagtcg tgaccgtgcg gggcccgcgg tttcgccggt gcctacggta cgtcaaccca
     4501 cggctgccga tctggtcaca gccacgctgc cgacggtcat gatcgtggac gactcgctga
     4561 ccgtgcgcaa gatcagcagt cggctgctgg cacgcgaggg ctaccaggta atgaccgcca
     4621 aggatggcgt cgacgcgcta gaacaactcg tcgccgtggt cccggacgtc atgctggtcg
     4681 acatcgagat gccacgcatg gacgggtttg aactgacccg aaacgtgcgt gccgatgcgc
     4741 gcctgcaaaa ggtgccaatc atcatcatca cctcgcgaac cgctgacaag caccgtcaat
     4801 acgcccttga gctcggcgtg aaccactatc tcggcaaacc gtaccaggaa gacgaactgc
     4861 tgcgcctggt agcggggcac gtcagggcgc agcgccagtc ttgaccactg cgtagcgctg
     4921 cagtgtccga tgcctggcct gagcatggtc gaccaacggt gccggcgtgt cgcgaccgat
     4981 gcgcacgccg caggcggcct gctccggctc actcatgcgc caaggcgcat gcacccactg
     5041 gttcgggaca ctggccagct cgggaagata acgacggatg aacctgccgg cgggatcaaa
     5101 gcgctccgat tgcgtcaccg ggttgaagat gcggaaatag ggttgcgcgt cgcagccgct
     5161 tgatgccgcc cattgccatc cgccattgtt ggccgacaga tcgaaatcgt tgagctgact
     5221 ggcaaagtag ctttcgccgc gccgccagtc aatgcccaga tccttggcca gaaaggaggc
     5281 cacgagcatg cgcagacggt tatgcatgta gccggtactg ttgagttgcc gcatggcggc
     5341 atcgaccagc ggatagccgg tgcggccagc gcaccaggcg gcgaaggccg cgtccgcgtg
     5401 cggtccctgt tcccagacga tggcgtcgta cggcggcttg aacgagcgct cgctgacgta
     5461 tggaaaacgg tcgagaatca tgaagtagaa atcgcgccag acgagttcgg aaagccaggt
     5521 cgctgcaccc ccggtcggcg catgcagggc gccggcggca atcgcctgcg ccaccagctc
     5581 gcggaccgag agggtcccga agcgcagatg caccgagaga tacgacaccc ccttgaccgc
     5641 tggaaaatca cgctgctccc ggtaccgagc catgcgcccc agaaagtcgc tcaggcgctg
     5701 ccggccaccg gacatccccg gcgcgatgcc gagtgcgcgc agatcgctgg cggcgaaacc
     5761 gagttccgcc agcgtcggca cgccaccgcc caccggcgcc acggcgagcc cccggccacg
     5821 gccggcatgc ggctgccagt cgtcctcccc gaggcgtctg agccaggcgt tccggtaggg
     5881 tgtgaacacg gtataaggac gcccggcctg cgtcagcacc tcatcagcgt cgaacagcgc
     5941 ctgatccttg aacgactcga aggcaatgct gtccgcctgc aaaagcgcgg cgaccgcact
     6001 gtcgcgtcgc ttggcctgcg gttcgtagtc acgattggca aacaccgcgg ccacaccaag
     6061 cgatctcgcc aactcgggaa tctcccgagt gacccaaccg tggcgaacga tcagtgcgcc
     6121 gccgcgtacg cgcagcgccg cgtcgagttc gaccagcgac tcgcggatga actcgacgcg
     6181 acggtcgctg cgtcgctcca gggtgtcgag aatctcccgg tcgaaaacga aagcgcaata
     6241 gacctgtcgt ccacgccgca gcgcttcgga aagcgcggcg tgatcgtaat cacgcagatc
     6301 gcgccgaaac cagacaagaa tcgcctccag catgctttcc cccaatggta gacccagccc
     6361 gcgcggacgt gcccatctcg cctctgtcat cgtcaggaca agcgccaggg accgcaaaat
     6421 caggcatgat gttcgtgttt gccggaatcg gacaaggcga tgaacgtttg tgtcagccgc
     6481 cagcgccgtt ta
//
LOCUS       DAOSIT010000057         6374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11034_length_6374_cov_7.485678, whole genome shotgun sequence.
ACCESSION   DAOSIT010000057 DAOSIT010000000
VERSION     DAOSIT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11034_length_6374_cov_7.485678"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLL14_02455"
     CDS             complement(<1..758)
                     /locus_tag="PLL14_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOG02811.1"
                     /translation="MISSDLFDQWQSGLADGSIVPYLGPGVLADVHSELDGRSIPATS
                     EQLILALNGGRPMAPKLMYEFPRAAMNVELKRGRSAVTRFLDSTYGDTRWTRAAVHDW
                     LATIRPPYVIDINRDTQLLDSYAGTPHLLVLGCARLGGTDYRFTLHVSDGSSYRAIAP
                     EEADAGLPVLFKPMGCPLPQPSYIASDADYVDYITELMGGFAVPSFIKARRRGKRYLL
                     LGMRLNRDTERMVLSDLIHDAAADSAGWALIAEA"
     gene            1135..>1394
                     /locus_tag="PLL14_02460"
     CDS             1135..>1394
                     /locus_tag="PLL14_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOG02812.1"
                     /translation="MPPSHDKMIDAGHLDAFASLANTTLDGTERLLSLNLQAARSLLS
                     DALTHGKALSEASDLNEVLSINAAMSQPFLEKTLWYWRRCYE"
     assembly_gap    1395..1494
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1495..2687)
                     /locus_tag="PLL14_02465"
     CDS             complement(<1495..2687)
                     /locus_tag="PLL14_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOG02813.1"
                     /translation="MHHLPRSGQGHGFATRVIHAGQSPDPSTGAVIPPIYATSTYVQQ
                     SPGVHQGLDYGRSHNPTRWAFERCIADLESGAKAFAFASGLAAIATVLETLDSGAHVI
                     AGDDLYGGTYRLLEGVRRRTAGLAATFVDLADAEALTAAVRPTTRMLLVETPTNPLLK
                     LADLEALAAFCRRHELISVVDNTFATPWIQRPLELGFDIVLHSTTKYINGHSDVIGGV
                     AVVSGEARNAALRERLAFLQNAVGAISGPFDCFLALRGVKTLDLRMQRHCENAQELAA
                     WLERQPRVRRVIYPGLASHPQHQLARRQMRAYGGMIAFELAADLAGTRCFLEATEVIT
                     LAESLGGVESLIEHPAIMTHATIPPEQRAQLGISDSLVRLSVGIEDVADLRSDLENAL
                     GRLPD"
     gene            complement(2942..3727)
                     /locus_tag="PLL14_02470"
     CDS             complement(2942..3727)
                     /locus_tag="PLL14_02470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOG02814.1"
                     /translation="MSSDYSRQEMMAIATGREIRDGELAIFGVGLSMLAGYFAQACHA
                     PRVRAMTEGGVYGATPVGGLPWGIECNRLAVNAVSFTSALDALGCLVASGRCDVGIIG
                     AAQVDRFGNVNTTGIWDGPIGESYRPPKTRLTGAGGANDIACGAKRYLIMVSHEPKRF
                     VDRVSYISSPGYLDGGDARERVGFVGGGPSAIITTLGILRPDPQSHEFQLDAYFPFSG
                     VDEILANTGWNLQVSAGVQVVPEPTLQELAALRRVDQTGMLRR"
     gene            complement(3724..4713)
                     /locus_tag="PLL14_02475"
     CDS             complement(3724..4713)
                     /locus_tag="PLL14_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOG02815.1"
                     /translation="MSTKVITLAEAVRRYTWDGMQYASGAALPVGSDAIAFGRELLRQ
                     KRRDLHAIFHANTQQLNLLAAAGAVARAECGFSGLEGFGFANGLRRAVEQGPLVLEDY
                     SNLGMTLRLLGGALNWPFVPATVNIGSDLQDRRAFEPAIHPNRNKIPAVTDPFSGRRI
                     GAFSPLRPDLAAIHVTLADSDGNAIMLGTEWSRFELSRAAKRVVLLADALVHTDCMRQ
                     YPNLVRIPGIIVEAVVYWPFAAWPQASPGMYDVDETHMKAMNEALGSEQGAASYLCDY
                     VDSYADLDGYLERIGAGKRAELSNGATAFLLDPYQRWLLTHAEAQALSTGGEA"
     gene            complement(4726..>4993)
                     /locus_tag="PLL14_02480"
     CDS             complement(4726..>4993)
                     /locus_tag="PLL14_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOG02816.1"
                     /translation="PVIGLRGNAEATARLISQLQLVAEKRPSGKAGYTLDHTAGVFLF
                     DGGGRLRGVAPFGQSAADLRSDLAILLAEGASRQTAAQRLSRAN"
     assembly_gap    4994..5093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5094..5407)
                     /locus_tag="PLL14_02485"
     CDS             complement(<5094..5407)
                     /locus_tag="PLL14_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG02817.1"
                     /translation="MLFERSVDDLQREIRAAGADGKQLAVVLSLPDCPGCLEMEKTVY
                     RDMHTQRVIERRFRTVHLDLARSEPITDSKGRATTAADLAKGLRAFGTPSFVFFAVDG
                     SLL"
     gene            complement(5502..6335)
                     /locus_tag="PLL14_02490"
     CDS             complement(5502..6335)
                     /locus_tag="PLL14_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02818.1"
                     /translation="MSDYIALANQDLPSQPMPGALDGPPLSAPRWPGRALERVAVWVV
                     LLLAWSVASVHLPLGDNPLLPSPLITAKALWESMPELWAGTLSSLTILLPGFALATLL
                     GIVTGLAVGTSLRLQRMFLPFAKVAGPVPPTVYIPYAIAILPTFRLSAIFVVFIGAFW
                     PIFQNAVAGAHALEQRYRDNARILGLRGFEYQRRVVFPAALPHIFTGMAVGLAFSFIL
                     LTVAELFGANAGLGRFVQYYADFADYPRMVAGILYTGLVTFLAMTLLDRVRRRSLFWL
                     R"
BASE COUNT         1181 a         1928 c         2056 g         1009 t
ORIGIN      
        1 ttggcctcgg cgatcaatgc ccagccggcg gagtcggcag cggcatcgtg gatcaggtca
       61 gagaggacca tccgctcggt gtcgcgattc aggcgcatgc cgagcaggag ataacgcttg
      121 ccgcgacgac gcgccttgat gaacgaggga accgcgaagc cgcccatcag ttcggtgatg
      181 tagtcgacgt agtcggcatc ggaagcgatg taactgggtt gcggcaacgg acaacccatc
      241 ggtttgaaaa gcaccggcaa gccggcatcg gcctcctccg gagcgatggc gcggtaactg
      301 gaaccatccg aaacatgcag ggtgaaacga tagtcggtac cgccaaggcg agcgcaaccg
      361 aggaccagca ggtgtggagt cccggcatag gaatcgagca gctgcgtgtc gcggttgatg
      421 tcgatcacat acggcggccg gattgtcgcg agccagtcgt gcaccgcggc gcgcgtccag
      481 cgcgtgtcgc cgtaggtgct gtcgagaaag cgcgtcaccg cgctgcgacc gcgcttgagt
      541 tcgacgttca tcgccgcgcg cggaaactcg tacatcagct tcggtgccat cgggcgtccg
      601 ccattcaggg ccaggatcag ttgctcactg gtggcgggaa tacttcgacc atcgagttcc
      661 gagtgtacgt cggcaagcac gcccggcccg agataaggaa cgatgctgcc gtcggcaagg
      721 ccggactgcc attgatcgaa aaggtcgctg gaaatcattg tgcgtactcc gagtggattc
      781 acaaggtcca cgcaagaaat acgcctgccc tgcaggcccg tgcctgcagg gttgccccac
      841 ccgcggccct tgccgatgtc ggctttgcga caattgccat catgctctcc tgccggcctt
      901 gcggggtgtt cttgccagca cggcgaagga gaccggaatg cgtcggccgc agccgtcgct
      961 accgccgttc cggaaaaggg agacgatgcg gtggaaaaaa atccgttggc caggagcggg
     1021 gaaaggcgta atctaccctg acgcatcagc gcagccattc cggtggaagt cggtcgtcca
     1081 ggacgcggct tcaggaatga cgggatcgac tcaccagaac gactggaacc tgccatgccc
     1141 ccttcccacg acaaaatgat cgacgccggt cacctcgacg cgttcgcgtc cctggccaat
     1201 accactctcg acggtacgga gcggttgctg agcctcaacc tgcaggcggc tcgctccttg
     1261 ctgagcgatg cactgaccca cggcaaggct ctcagcgagg ccagcgacct taacgaggtg
     1321 ctgtcgatca atgcggcgat gagccagcct tttctggaga agacgctctg gtattggcgc
     1381 cgctgctacg aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntagtcg
     1501 ggaagcctgc cgagagcgtt ttcgaggtcg cttctgaggt cggcgacatc ctcgatgccg
     1561 acggaaagac gaaccagtga atcgctgatg ccaagttggg cgcgttgctc gggcggaatc
     1621 gtcgcgtggg tcatgatcgc cggatgttcg atcaggcttt cgacgccgcc aaggctctcg
     1681 gcaagggtga tcacttcggt cgcttcgagg aagcaacggg taccggcaag atcggcggcg
     1741 agttcgaaag caatcatgcc accgtaggcg cgcatctgcc gtctggccaa ctggtgttgc
     1801 gggtgtgagg cgaggccggg gtagatcacg cggcgcactc gcggctgccg ttccagccag
     1861 gcggccagtt cctgggcatt ttcacaatgc cgctgcatgc gcagatcgag cgtcttgacg
     1921 ccacgcagag ccaggaagca gtcgaaaggt ccgctgatcg caccgacggc attctgcaga
     1981 aaggcgagcc gctcgcgcaa cgccgcattg cgggcttcgc cgctgacgac cgcgacgcca
     2041 ccgatgacgt cggagtggcc gttgatgtac ttggtcgtcg agtgcaggac gatgtcgaat
     2101 ccaagttcca gcgggcgctg gatccacggc gtggcaaagg tgttgtcgac caccgagatg
     2161 agttcatgcc gccgacagaa ggccgcgagc gcttcgaggt cggcaagttt cagcagcggg
     2221 ttggtcggcg tttcgacaag cagcatacgg gtcgtcgggc gcacggcagc ggtcagcgcc
     2281 tcggcatcgg cgaggtcgac gaaagtggcc gcgagcccgg cggtgcgccg acgcacccct
     2341 tccagcaggc ggtaggtacc accgtagagg tcgtcgccgg cgatcacgtg cgcgccgctg
     2401 tcaagcgttt ccagcacggt ggcgatcgcc gccagtccgg aggcgaaagc gaaggccttc
     2461 gccccggatt cgagatcggc gatgcagcgc tcgaaggccc agcgggtggg gttgtgcgag
     2521 cgaccgtagt ccagtccctg gtggacacca gggctttgct gaacataggt cgaagtggca
     2581 tagatcggcg ggatgacggc gccggtgctg ggatcgggcg attgcccggc gtggatgacc
     2641 cgggtggcaa agccatggcc ttgcccggag cgtggtaggt ggtgcatgcg catgcctttt
     2701 ctgtaaaggg ttgatgggtg gaatcgaagc ggtgcagcag gcccgtaatc aaagctgatt
     2761 gtcggtgttg ccggcgacgg ggagaggcga ccgggattta ccggaattgt gacacgacgg
     2821 tgccggtggc gcggccggat ttcgccgtgc cggccgggca aatctcccgg tcggatcaac
     2881 tcgccgaccg acgcggtttc ctgccagtaa ccgtgcttcg tccccaatac cgccccactt
     2941 tttaccggcg cagcatgccc gtctggtcca cacggcgcag ggccgcgagc tcctgtaagg
     3001 tcggttcggg aacaacctgc accccggccg acacctgcag gttccagccg gtgttggcga
     3061 gaatctcgtc gacaccggaa aacgggaagt aggcgtcgag ctggaattcg tgactctgtg
     3121 ggtcgggccg caggatgcca agggtggtga tgattgccga agggccgccg ccgacaaaac
     3181 ccacccgctc cctggcgtcg ccgccgtcga gatatcccgg cgaagaaatg tagctgaccc
     3241 gatccacgaa acgttttggc tcgtggctga ccatgatcag gtagcgcttg gcaccgcagg
     3301 cgatgtcgtt ggcgccgccg gcgccggtca ggcgagtctt cggcgggcga tagctttcgc
     3361 cgatcgggcc atcccagata ccagtggtat tgacgttgcc gaagcgatcg acctgcgccg
     3421 cgccgatgat gccgacgtcg cagcgaccgg acgcgaccag gcaaccgaga gcgtcgagcg
     3481 cactggtaaa actcacggca ttgaccgcca gacgattgca ttcgattccc cagggcaggc
     3541 cgccgacggg cgtcgcgccg tacacgccgc cttcggtcat cgcccgcacc cggggcgcat
     3601 gacacgcctg cgcgaagtag ccggcgagca tcgacaggcc aacgccgaag atggccagtt
     3661 cgccatctct gatctcgcgg ccggtggcga tggccatcat ctcctggcga ctgtaatcgc
     3721 tgctcatgcc tcgcctccgg tgctcagggc ttgtgcctcg gcgtgcgtca gcagccagcg
     3781 ctggtagggg tcaagcagga aagccgtcgc cccgttcgac aactcggccc gcttcccggc
     3841 gccgatgcgc tcgaggtagc catcgaggtc ggcgtagctg tcgacatagt cgcagaggta
     3901 agaggccgcg ccctgctcgc tgccgagggc ctcgttcatg gccttcatgt gcgtttcgtc
     3961 aacgtcgtac atgccgggcg aagcctgcgg ccaggcggcg aagggccagt acacgacggc
     4021 ctcgacgatg atcccgggga tccgcaccag gtttggatac tggcgcatgc agtcggtgtg
     4081 caccagcgca tcggccagca gcacgacccg cttcgccgcc cgcgacagtt cgaagcggct
     4141 ccactcggta ccgagcatga tcgcgttgcc atcgctatcg gcgagcgtca cgtggatcgc
     4201 cgccagatcc ggacgcagcg gcgagaaagc accgatccgc ctgcccgaga agggatcggt
     4261 caccgccgga atcttgttcc ggttggggtg aatagccggc tcgaacgcac gtcggtcttg
     4321 aagatccgag ccgatattga ctgtcgccgg aacgaatggc cagttcagcg caccacccag
     4381 cagacgcagt gtcatgccca gattggagta atcctcaagc accaacgggc cctgctcgac
     4441 tgcccgccgc aagccgttgg caaagccgaa gccctccagc ccgctgaagc cacactcggc
     4501 acgggccacg gcaccggccg cagcgagcag gttgagttgc tgggtattgg cgtgaaaaat
     4561 ggcgtgcagg tcgcggcgct tctggcgcag cagctcccgc ccgaaggcga tggcatccga
     4621 gccgaccggc agcgccgcgc cgctggcgta ctgcatgcca tcccaggtgt atcgacggac
     4681 ggcttcggcc agggtaatca ctttggtgga catggtcgct tctcttcaat tggcccggga
     4741 aagccgctgc gctgccgtct gcctgctggc accttcagcc agcagaatgg ccagatcgct
     4801 ccgcaagtcg gcggcgctct ggccaaatgg agcgacgccc cgcagccgac caccgccatc
     4861 gaacaggaat acgccggcgg tatggtcgag ggtatagcca gctttccccg agggccgctt
     4921 ttcggccacc agttgcagtt ggctgatcag tcttgccgtc gcctcggcgt tgccgcgcag
     4981 cccgatgacc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagaagac
     5101 tgccgtccac ggcaaagaag acgaacgatg gcgtgccgaa cgcgcgcagg cccttcgcca
     5161 gatcagcggc ggtcgtggcc cgccccttgc tatcggtgat cggctcggaa cgggcgagat
     5221 ccagatgaac ggttcggaag cggcgctcga tcacgcgctg cgtgtgcatg tcgcgataga
     5281 ctgttttttc catttccagg caacccgggc aatccgggag ggacagcacg accgccagtt
     5341 gcttgccatc cgcgccggcc gcccgtatct cgcgctgcag gtcgtcgacg ctgcgctcga
     5401 acagcaccgc atgcgccacg ccgacccaca ggccgagcgc caggccgctg ccgagtcgca
     5461 gccaggccgc gacggtggcc cgccatgcac gacgccgcat gctatcgcag ccagaacaag
     5521 gaccgccgcc gaacgcggtc gagcaaggtc atcgccagga aggtcaccag gccggtatag
     5581 aggatgccgg cgaccatccg gggatagtcg gcgaagtctg cgtaatactg gacaaagcgg
     5641 ccaagaccgg cgttggcgcc gaacaactcg gcaacggtga gcaggatgaa cgagaaggcg
     5701 aggcctaccg ccatgccggt gaaaatgtgc ggcagcgccg ccgggaaaac cacgcgccgc
     5761 tgatactcga aaccgcgcag cccgagaatc cgggcattgt cccgatagcg ctgctcgagc
     5821 gcgtgtgcgc cggcaacggc attctggaaa atcggccaaa aggcaccgat gaagacgacg
     5881 aagatcgccg acaatctgaa agtgggtaga atggcaatcg catagggaat ataaaccgtc
     5941 ggcggtaccg gcccggcaac cttggcgaaa ggcaggaaca tccgctgcag ccgcagcgac
     6001 gtccccaccg ccagccctgt gacgatgccc agcagcgtcg ccagggcgaa tccggggagc
     6061 aggatggtca gtgacgacag ggtgcccgcc cacagttcag gcatcgattc ccagagggct
     6121 ttggcggtga tcagtggcga tggcagcagc gggttgtcgc ccagcggcag atggactgat
     6181 gcgaccgacc aggcaagcag cagcaccacc cagacggcta cgcgttccag cgcacggcca
     6241 ggccagcggg gggcggaaag gggcggcccg tccaatgctc caggcatcgg ctgggacgga
     6301 agatcctggt tcgccagggc gatatagtcg ctcatcggtg gttctcctcg tactttcagg
     6361 tcgcggccga acag
//
LOCUS       DAOSIT010000058         6368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11053_length_6368_cov_8.155869, whole genome shotgun sequence.
ACCESSION   DAOSIT010000058 DAOSIT010000000
VERSION     DAOSIT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11053_length_6368_cov_8.155869"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /gene="trmE"
                     /locus_tag="PLL14_02495"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
     CDS             <1..152
                     /gene="trmE"
                     /locus_tag="PLL14_02495"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480973.1"
                     /note="in Escherichia coli this protein is involved in the
                     biosynthesis of the hypermodified nucleoside
                     5-methylaminomethyl-2-thiouridine, which is found in the
                     wobble position of some tRNAs and affects ribosomal
                     frameshifting; shows potassium-dependent dimerization and
                     GTP hydrolysis; also involved in regulation of
                     glutamate-dependent acid resistance and activation of
                     gadE; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG02819.1"
                     /translation="IEVGRERLPQLELFAEELRLAQSSLSTITGEFTADDLLGEIFGR
                     FCIGK"
     gene            complement(313..3522)
                     /locus_tag="PLL14_02500"
     CDS             complement(313..3522)
                     /locus_tag="PLL14_02500"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOG02820.1"
                     /translation="MSPHAYSEDQLVEQPAIGLFGELGWQTVSALEETFGAGGLLGRE
                     TRGEVVLVERLRTALVRFNPTLPPEAIANAVDELTRDRSAMSLEAANREVYRLLKEGI
                     TVSVPDREHGGQSGGQCTERLRVVDWEHPENNDFLLVSQLTVTGPLYTCRPDLVGFVN
                     GVPWVVIELKKPGVPARAAFDGNLTHYKQQIPALFWSNALLIASNGTDSRVGSLTADW
                     GRWVEWKRIEREDEARRVSLEVMLRGTCDPVRLLDLVENFTLFSEHKAGLVKIIGQNH
                     QFLGVNNAVRKMLEARKLGHGRGGVFWQTQGSGKSFSMVFFAQKVLRKLAGNWTFVVV
                     TDRVELDEQIAKTFKTTGAVSEAEGDECHAASGAHLRELLRGNHRYVFTLVHKFQTPE
                     LLCDRSDVIVLTDEAHRSQYDTLALNMRAALPRAMFLAFTGTPLIAGEERTKEVFGDY
                     VSIYDFQQSIEDGATVPLFYENRTPELQLVNPDLNEDIYRLIEDAELDAEQEARLEKV
                     LGRQYHLITRDDRLETVARDIVRHFLDRGMLANAVGKAMVVSIDKATALRMHDKVKVH
                     WAAATARVQKELGELHYQPGGDMTPEQARRDARMAELKQRLSVLTSTDMAVIVSPGQN
                     EIQQMQQLGLSIEPHRKRMNESQPGLDERFKDTDDPLRLVFVCAMWLTGFDAPSCSTV
                     YLDKPMRNHSLMQAIARANRVFPGKHSGVIVDYANVFAALEKALAIYGAGQEGKTPVR
                     DKQQLVEELRKAVVDATAFCAAHGVMLGEIEATPAGSIERLSRIQDGINALISPDPLR
                     RDFFTQQRLVSTLFHAVKPDPSALESASRVACLATLADAIRARLHPNPAEISQVMGRI
                     NGLLDASITGHKVRQPGPPGLDLSKINFEALAQRFKESRHRNTDLEVLKAAIRAQLEQ
                     MIRLNRTRADFAERFAALIESYNAGSATIEALYAELLALSKGLNDEQERHVREQLSEE
                     ELVLFDILTRPAPELSTAERAEVKKVARELLTRLKTLLVLNWRQKSAARSQLKLTIED
                     TLDNGLPRAYTPELYNRKCSAIFEHMFESYSERSAGIYA"
     gene            complement(3519..4226)
                     /locus_tag="PLL14_02505"
     CDS             complement(3519..4226)
                     /locus_tag="PLL14_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02821.1"
                     /translation="MIAGDPNVQSVELVAAALGDLCDELVLVGGCAASLLIDTPTAPP
                     PRVTYDVDLIAVVAALRNYHALEEQFAERGFERDVSADAPICRWRIGAIEVDLMPTDE
                     AVLGFSNRWYAEAAATAMRLTLPSGTAINLISAPAFLATKFEAFRTRGKADLLLSHDF
                     EDIINVVEGRFSIVEEVDAGGAALRTYLSQQFASIIAAPDYTNVLPGLVAFDDLHSQR
                     IERVRQRIAALAAMESR"
     gene            complement(4223..4756)
                     /locus_tag="PLL14_02510"
     CDS             complement(4223..4756)
                     /locus_tag="PLL14_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02822.1"
                     /translation="MPRLPNRQPVLKPQDFYLLLALAACRDQGTTYPELAAFSGLSMS
                     EVHGALKRAEVARLLFFDDKKPRILLPAFKEFLYHGARYAFPALRGALVVGLPTAYAA
                     APLAAHIAPSSDPVPVWPFIGGTVRGGSVIPLYSSAPAAAARSPALYENLALFDALRM
                     GNARERALAQELFEARL"
     gene            complement(4832..6109)
                     /locus_tag="PLL14_02515"
     CDS             complement(4832..6109)
                     /locus_tag="PLL14_02515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG02823.1"
                     /translation="MVVARLDWPTKRLSTLAEFRNGVNYNKSSFGEGVKVVGVSNFQD
                     YSKPRYAELEQINPDGIVSERNILRDGDIVFVRSNGNRELIGRSLFIEKPPEEVTHSA
                     FTIRLRFTSPDVYPKFYAYCFRTPLIRQALTAQGGGTNINNLNQDILNGLEVPLPPLA
                     VQQRIAGILSAYDELIENSQRRIRILESMARALYREWFVHFRFPCHENQPCVASPLGE
                     IPQGWEVKKLKDVCRLTMGQSPKSEFYNEVGDGEPFHQGVTNFGDRFPTDRLFCTAEG
                     RIAEAGDILFSVRAPVGRMNIANKRIILGRGLSAIRYNQGSQAFLWEQLRNRFTRDDM
                     MGNGAIFASVTKDDMQGIELVCPPPSVIEAATNHFEPLHSEIATLSQQVQNLRRTRDL
                     LLPRLLSGQVAVDAIPDPDSPFANSESGQGVSA"
     gene            complement(6109..>6368)
                     /locus_tag="PLL14_02520"
     CDS             complement(6109..>6368)
                     /locus_tag="PLL14_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOG02824.1"
                     /translation="EIFASPPVYRDIPGLCKAATLAEIAAQGWSLNPGRYVGVAPGED
                     VSDEDFKEQLETLNKELEALNVQARELEQVIAANVAGILEA"
BASE COUNT         1133 a         1987 c         2015 g         1233 t
ORIGIN      
        1 gcattgaggt cgggcgcgag cgcttgccgc aactggaact gtttgccgag gaactcaggc
       61 tggctcagtc gtcgctgagt accatcaccg gcgaattcac cgccgacgac ctgctcggag
      121 agatcttcgg gcgattttgt atcggaaaat agcgaggctt tcgcgcaacg ccgttatcga
      181 tacccttgcc ggcaaacatg caccggcttg ggcccaagcc ctcctgtcgt cggcgggtaa
      241 accaagcacc gccatggcga catccgccac gtgccgcagg cccagcacca ctccgatgtg
      301 ggtcacgcga tctcaggcat aaatccccgc gcttcgctcg gagtagcttt caaacatatg
      361 ctcgaaaatc gccgagcatt tccggttgta gagttccggt gtgtaggcgc gtggcaagcc
      421 gttgtccagg gtgtcttcga tggtgagctt caactgtgac cgggccgccg atttctgtcg
      481 ccagttgagc accagcaggg ttttgagtcg cgtcagcagc tcccgcgcca ccttcttcac
      541 ctcggcgcgc tccgcggtgc tcagttccgg cgcggggcgg gtcaggatat cgaagaggac
      601 cagttcttcc tcgctcaact gttcgcgcac atgccgttcc tgttcgtcat tcaggccctt
      661 gctcaaggca agcagttcgg catacagcgc ctcgatggtc gcgctgccgg cgttgtagct
      721 ctcgatcagc gccgcgaacc tttcggcaaa gtcggcgcgg gtccggttca accggatcat
      781 ctgttccagt tgagcgcgga tggcggcctt gagcacttcg agatcggtgt tcctgtgtct
      841 ggattccttg aagcgctgtg ccagcgcctc gaagttgatc ttcgagagat cgagccccgg
      901 cggccccggc tgccgcacct tgtgaccggt gatggatgcg tcgagcaggc cgttgatccg
      961 ccccatcacc tgcgagattt cggccgggtt cgggtgcaac ctggcgcgga tggcatcggc
     1021 cagtgtcgcc aggcaggcga cgcggctggc ggattccagc gctgatgggt cgggtttcac
     1081 ggcgtggaag agcgtgctga ccagccgttg ctgggtgaag aaatcgcggc gcagcgggtc
     1141 gggggagatc agcgcgttga tgccgtcctg aatgcgcgac agacgctcga tgctaccggc
     1201 gggtgtcgct tcgatctcgc ccagcatcac gccgtgcgcg gcacagaaag ccgtggcatc
     1261 gaccaccgcc ttgcgcagct cctcgacaag ttgttgcttg tccctgaccg gagtcttgcc
     1321 ttcctggccc gcaccgtaga tggccagcgc cttttccagc gcggcaaaga cgttggcgta
     1381 atcgacgatc acgccgctgt gcttgccggg gaagacgcgg ttggcgcggg cgatagcctg
     1441 catcagcgag tggttgcgca tcggtttgtc gagatacacc gtcgagcagc tcggtgcgtc
     1501 gaagccggtc agccacatgg cgcagacaaa gaccaggcgt agcgggtcgt cggtgtcctt
     1561 gaacctctcg tccagccccg gctgcgactc gttcatgcgc tttcggtgcg gctcgatgct
     1621 gagaccaagc tgttgcattt gctggatttc gttctgcccc ggcgacacga tcaccgccat
     1681 gtcggtgctg gtcagcaccg atagccgttg tttcagttcg gccatgcgcg catcgcgtcg
     1741 tgcctgttcg ggcgtcatgt ccccgccggg ctggtaatgc agttcgccca gttccttctg
     1801 cacccgcgcc gttgccgccg cccaatgcac cttcaccttg tcgtgcatgc gcagcgcggt
     1861 ggccttgtcg atggacacga ccatcgcctt gccaaccgca ttggccaaca ttccccggtc
     1921 gagaaagtgc cgcacgatgt cccgcgccac cgtttccagt cggtcgtcgc gagtgatcag
     1981 atggtattgc cgcccgagca ccttctccag tctggcttcc tgctcggcgt cgagctcggc
     2041 gtcctcaatc agccggtaga tgtcttcgtt gaggtcggga ttgacgagct gcaattccgg
     2101 cgtgcggttc tcgtagaaca gcggcacggt ggcgccgtcc tcgatggact gctggaagtc
     2161 gtagatcgaa acgtaatcgc cgaacacttc cttggtgcgc tcctcgccgg cgatcagcgg
     2221 cgtgccggta aaggccagga acatcgcacg gggcaaggcg gcgcgcatgt tgagcgccag
     2281 cgtgtcgtac tggctgcggt gcgcttcgtc ggtcagcacg atcacgtccg agcggtcgca
     2341 cagcaactcc ggcgtctgga acttgtgcac cagcgtgaac acgtagcggt ggttgccgcg
     2401 cagcagttcg cgcaggtgcg cgccgctggc ggcgtggcat tcgtcgccct cagcctcgct
     2461 cactgcgccg gtagtcttga aggtcttggc gatctgctcg tcgagttcga cgcggtcggt
     2521 gaccaccacg aatgtccagt tgccggcgag cttgcgcagc accttctgcg cgaagaagac
     2581 catcgaaaag ctcttgccgc tgccctgcgt ctgccagaac acgccgccgc gcccgtggcc
     2641 cagcttgcgc gcttcgagca tcttgcgaac ggcgttattg acgccgagga actggtggtt
     2701 ctggccgatg atcttgacca gcccggcctt gtgctcggaa aacaaggtga agttctcgac
     2761 cagatcgagc aggcggacgg ggtcgcaggt gccgcgcagc atcacctcca gcgacacgcg
     2821 gcgcgcttcg tcctcgcgct cgatgcgctt ccactccacc cagcggcccc agtcggcggt
     2881 gagcgagccg acgcggctgt cggtgccgtt gctggcgatg agcagcgcgt tactccagaa
     2941 cagcgccgga atctgctgct tgtagtgggt gaggtttccg tcgaaggcgg cgcgagccgg
     3001 aacgccgggt ttcttgagtt cgatcaccac ccagggcacg ccattgacga agccgaccag
     3061 gtcgggccgg caggtgtaca gcgggccggt cacggtcaac tggctgacga gcaggaagtc
     3121 gttgttctcc gggtgttccc aatcgaccac acgcaggcgc tcggtgcatt ggccaccact
     3181 ctggccgcca tgttcgcggt cggggaccga caccgtgatg ccttccttga gcagccggta
     3241 gacctcgcgg ttcgccgcct ccaggctcat cgccgagcgg tcgcgcgtca actcgtcgac
     3301 cgcgttggcg atcgcttccg gcggcagggt cgggttgaac ctgaccaaag cggtacgcag
     3361 gcgctcgacc agcaccacct cgcccctggt ttcccgcccc agcaaaccgc ccgcgccgaa
     3421 ggtctcctcc agcgccgata ccgtctgcca gccgagttcg ccaaacagcc cgatggcggg
     3481 ttgctcgacg agctggtctt cggagtaggc gtgcgggctc atcgcgactc catggcagcg
     3541 agcgccgcga tgcgctggcg aacgcgctcg atgcgctgcg aatggaggtc gtcgaaagcg
     3601 accagccccg gcagcacgtt cgtgtaatcc ggcgccgcga tgatgctggc gaactgctgg
     3661 gagaggtaag tgcgcagcgc cgctccgcca gcgtcgacct cttcgacaat ggagaagcgg
     3721 ccctcgacca cgttgatgat gtcctcgaaa tcgtggctca gcagcaggtc ggccttgccg
     3781 cgcgtgcgga atgcctcgaa cttggtagcc aggaaagccg gcgcggaaat caggttgatc
     3841 gccgtgccgc tgggcagcgt caggcgcatc gccgtggccg ccgcttccgc ataccagcgg
     3901 ttggaaaagc cgagcaccgc ttcgtcggta ggcatcaaat ccacttcgat ggcgccaatg
     3961 cgccagcggc agatcggcgc atcggccgac acatcgcgct cgaaaccccg ttcagcgaac
     4021 tgttcctcca gcgcatggta gttgcgcagc gcggcaacca cggcgatcag gtccacgtcg
     4081 taggtcacgc gcggcggtgg cgcggtcggt gtgtcgatca acaggctggc cgcgcagccg
     4141 ccgaccagta ccagttcatc gcaaagatca cccagcgcgg cagcgaccaa ttcgacgctc
     4201 tgcacattgg gatcgccggc aatcacaggc gcgcctcgaa caactcttgc gccagcgccc
     4261 gttcgcgcgc gttgcccatg cgcagcgcgt cgaacagggc caggttttcg tacagcgccg
     4321 ggctgcgggc ggccgcagcg ggtgccgacg aatacaacgg aatcacgctc cccccgcgca
     4381 ccgtgccgcc gatgaaaggc cacaccggca ccgggtcgga cgacggcgca atgtgggcag
     4441 cgagcggtgc cgccgcgtag gccgtgggca acccgaccac cagcgccccg cgcaaggctg
     4501 gaaaggcgta gcgggcgccg tggtagagaa actccttgaa ggctggcagc aggatgcgtg
     4561 gcttcttgtc atcgaaaaac agcaggcgcg ccacctcggc ccgcttcagc gcgccatgca
     4621 cctcggacat cgacagcccg gaaaacgccg ccagctccgg gtaagtcgtg ccctgatcgc
     4681 gacacgccgc cagcgccagc aggaggtaga aatcctgggg cttgagcacc ggctgacggt
     4741 taggaagtct gggcatggat caggcggcaa accgctgttt cgtgtttcgc gaaatgcgaa
     4801 agtacaaaat aaacaatgga aagtcaagaa ttcaagcgga tacaccctgc cccgattcgc
     4861 tattcgcgaa tggcgaatcg gggtcaggaa tcgcgtcgac cgcaacctgc ccggaaagca
     4921 agcggggcag cagcaggtcg cgggtacggc ggaggttttg aacctgctgg cttagtgtgg
     4981 cgatttccga atgcagtggc tcgaaatgat tggtggcagc ttcaataacc gatggcggag
     5041 ggcagaccaa ctcaatgcct tgcatgtcgt ccttcgtcac tgaggcaaat atggcgccgt
     5101 tgcccatcat gtcgtctctg gtgaagcggt tgcgcagttg ctcccataga aacgcttggc
     5161 tcccttggtt gtatcgaata gccgacaagc cacgccccaa gatgattctc ttgttcgcta
     5221 tgttcattcg tcctacaggt gcgcgcacac taaacagaat gtcaccggct tcggcgatgc
     5281 gcccctccgc tgtgcaaaac agtcgatcgg ttggaaaacg atcgccaaaa ttcgtaacgc
     5341 cttgatggaa aggctcgccg tcaccgacct cgttgtagaa ctcagatttc ggcgattgcc
     5401 ccatcgtgag gcggcaaacg tccttgagct ttttcacctc ccacccctga ggaatctcgc
     5461 cgagggggga ggcgacgcaa ggttggtttt cgtggcaggg gaagcggaag tggacgaacc
     5521 attcgcggta gagggcgcgg gccatcgact ccagaatcct gatgcgccgc tggctgttct
     5581 cgatgagttc gtcgtaggcc gacaggatgc ccgcgatgcg ttgctggacg gcgagcgggg
     5641 ggagcgggac ttcgaggccg ttgagaatgt cttggttgag gttgttgatg tttgtgccgc
     5701 caccttgagc agtcaatgct tggcgaatta gcggggttcg gaagcagtat gcgtagaact
     5761 tcggataaac gtcaggggat gtaaaccgca gccgaatcgt gaatgctgaa tgcgttacct
     5821 cttccggcgg cttttcaatg aagagacttc ggccgatcaa ctcacgattg ccattcgacc
     5881 gaacaaagac aatgtcgcca tcacggagaa tattgcgctc gctaacaatt ccgtccgggt
     5941 ttatttgctc aagctcggca tacctaggct tagagtagtc ctgaaagttg gagaccccga
     6001 cgactttcac cccctcgccg aagctgctct tgttgtagtt cactccgttc cggaattccg
     6061 caagggtgct aaggcgtttg gttggccagt cgagtctcgc caccaccatc acgcctccag
     6121 aatccccgcc acattcgcgg caatgacctg ttccagttcc cgcgcctgga cgttcagcgc
     6181 ttcgagttcc ttgttcaggg tttcgagctg ctccttgaag tcctcgtcgc taacgtcctc
     6241 gcccggcgcg acgccgacgt agcggccggg gttgagcgac cagccctggg ctgcgatctc
     6301 ggcaagggtc gcggccttgc acaggccggg aatgtcgcgg tagaccggcg gggaggcgaa
     6361 gatttcgc
//
LOCUS       DAOSIT010000059         6367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11056_length_6367_cov_7.152883, whole genome shotgun sequence.
ACCESSION   DAOSIT010000059 DAOSIT010000000
VERSION     DAOSIT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11056_length_6367_cov_7.152883"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PLL14_02525"
     CDS             <1..1438
                     /locus_tag="PLL14_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HOG02825.1"
                     /translation="RLAIAGDPLFLTVAEQVATEMNAWAEGFFGRPIIDDWRRQLDFT
                     TRIFRCRGLHLDDRHLRHSDGSGFSASITDLCLYVVNNQAALRVAGRSVVLYLPKIQT
                     AGEAALWNELLSALESHLGLPDGTIKAYVLVEQIEACFQLMEIRAALGRHFVGYNTGR
                     WDYINSVADACAWDSAFINPNIDAITMTYGYMRIYEDRVRRAVNTPDRNGRFALWQGG
                     METNIPVGTEAGVKAGMDRAVAGAEREQRAGASGKWVAHWKMVHIVRPVWERVGEANQ
                     LGREFPPLTYTPADAADLTLLEPSPRTVRGARDLISVALQYGNAFAQGQQAAALKPAD
                     FFLDDDVLYLMEDMATGEIRLSILWEWLHKGARLSADDPETGVRAGDPFTAELFTRLL
                     AEEHAKLLAARDKDVYDASKGTTLPIALAIVEAYVLRPEKAPWYIDLLNLNLNQHDLA
                     VARDRITRFFETFERDGIRITENLDFES"
     gene            1456..3714
                     /gene="aceA"
                     /locus_tag="PLL14_02530"
     CDS             1456..3714
                     /gene="aceA"
                     /locus_tag="PLL14_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HOG02826.1"
                     /translation="MERFAREVAATQEWFGSPRFEGITRLYTARQVVEQRGTIETDYP
                     VAREAAAAFHARLRELFAQKRSITTFGPYSPGQAVALKRLGIEGIYLGGWATSAKGST
                     TEDPGPDLASYPLSQVPDEAAVIVRALLTADRNQQYLRSRMSAEQRQKTPVYDYRPFL
                     IADADTGHGGDPHVRNLIRRFVEVGVTGYHIEDQRPGTKKCGHQGGKVLVPCDEQIKR
                     LNTARFQLDIMKVPGLIVARTDAEAANLLDGRGDERDQPFLLGVTNVSLPTYKTAWLA
                     LLRRFHALGVTALNGHLLYSLGEPEWRWADAWLERTGIAQAIANEVAGLDTGDALATD
                     AAFDRVANRFLDAWEGEARLRTYGEAVAEVLAFRESEGETLEMGVDAWRAFASTASLW
                     AARERARSLGVSVIWDCEHAKTPEGYYQIRGGIPYAIAKSLAVAPFADLLWMETKTAD
                     LADAREFAEAIHAVYPEKMLAYNLSPSFNWDSTGMSEDEMRRFPEELGKLGFVFNFIT
                     YGGHQIDGVAAEEFATSLREDGMLALARLQRKIRLVESPYRTPQTLVGGPRSDAALSA
                     CSGRTATTMAMGKGSTQHQHLIQTEVPKKLLAEWLTIWKEYHGLTLPIAVQLLPHRAG
                     SELLELALVGSNGGKLANVIFAAIQDRRERNILSVRDQNTFDPELRQKRLMTLIQLWL
                     IHRYRVDSVHYVTPTDDNEHQTEKMKEQGLFTDVNTEVGQIIVADVNATRVAELLAPD
                     RAALGRLIRKEG"
     gene            4329..4526
                     /locus_tag="PLL14_02535"
                     /pseudo
     CDS             4329..4526
                     /locus_tag="PLL14_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4502..5656
                     /locus_tag="PLL14_02540"
     CDS             4502..5656
                     /locus_tag="PLL14_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014035777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG02827.1"
                     /translation="MQATRIRLKNWRNFRTLDAPMRDVTYILGPNASGKSNLLDVFRF
                     LRDVSKPAGGGLQAAVIARGGISKLRCLHARRDPEVCIEMDLSDAPDDEVPNWRYVLG
                     FRPEGKGAQRILVSKEEVWHKGKPLFSRPDKDDIKDSVLLTQTRLEQIAANAEFRELA
                     EFFGSITYLHLVPQLLKYADQLGGRPLDNDPFGQSFLERIAKTTERVRNTRLRRISQA
                     LTFAVPQFKELRFVKDESGHPHLEALYSHHRPNAGWQSEEHFSDGTLRLLGLLWVLLD
                     GSSTLLMEEPELSLNDAVVKEIPLIIQRLLKNRKPKRQVLISTHSQALLSNPGIDGRG
                     VILLEVTADGSTGRTLQEDEALALKAGLSVAEVVLPKTRPSSVNQLEFWE"
     gene            5653..6264
                     /locus_tag="PLL14_02545"
     CDS             5653..6264
                     /locus_tag="PLL14_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02828.1"
                     /translation="MTSVALATEDELSEAVGVKLLSEHPVLAEAPPMLLRKNGLGYLR
                     SRMDSWRQMAHRQIVVVLTDLDKLACPLILLADWLGAGQQHPDNLLLRIAEREVESWL
                     LADHEALGKLLGRKSQFPGQPDTLPDPKQHLLRLAQKAPRAVREDLVAQQGTVARQGI
                     GYNRRLMDWVQTEWSPERAAARSPSLMRARNAMRETARRIMQA"
BASE COUNT         1240 a         1981 c         1962 g         1184 t
ORIGIN      
        1 ccggctcgcg atcgccggcg acccgctctt cctcaccgtg gccgagcagg tcgccaccga
       61 gatgaacgcc tgggccgagg ggttcttcgg gcgtccgatc atcgacgatt ggcgtcgtca
      121 gctcgacttc acgacccgca tcttccgctg tcgcggcctc catctcgacg atcgccactt
      181 gcgccacagc gacggctccg gcttttcggc ctcgatcacc gacctctgtc tctacgtggt
      241 gaacaaccag gcggcgctgc gggtcgcagg gcgctcggtc gtcctgtacc tgccaaagat
      301 ccagaccgcc ggcgaagccg cgctctggaa cgagctgctc agcgctctcg aaagccacct
      361 cggcctgccg gacggaacga tcaaggcata cgtcctcgtc gagcagatcg aggcctgctt
      421 ccagctcatg gaaatccgcg ccgcgctcgg ccggcacttc gtcggctaca acaccggccg
      481 ctgggactac atcaacagcg tcgccgatgc ctgcgcctgg gattcggcgt tcatcaaccc
      541 aaatatcgac gcaattacga tgacttacgg ctacatgcgt atctacgagg atcgcgtgcg
      601 gagggcggtc aacaccccgg accgtaacgg acgctttgcc ctctggcagg gcgggatgga
      661 gacgaacatt ccggtcggca ccgaggcggg cgtgaaagcg gggatggacc gtgcggtcgc
      721 cggcgcggag cgtgaacagc gtgctggggc gagcggcaaa tgggtggcgc actggaagat
      781 ggtccacatc gttcgcccgg tctgggagcg ggtgggcgag gcgaaccagc ttgggcgcga
      841 gttcccgccg ctgacctaca cgccagccga tgccgcggac ctgacgctgc tcgaaccctc
      901 cccgcggacc gtgcgcggtg cccgcgacct catctcggtg gccctccagt atggcaacgc
      961 cttcgcccag gggcagcagg cggcggcgct caagcccgcc gacttcttcc tcgacgacga
     1021 cgtcctttac ctgatggagg acatggccac tggcgagatc cgcctctcga tcctctggga
     1081 gtggctgcac aagggggccc gtctcagcgc cgacgacccg gaaaccgggg ttcgcgcggg
     1141 cgatcccttc accgccgaac ttttcacccg cctgctcgcc gaggagcacg ccaagctgct
     1201 cgcggctcgc gacaaggacg tctacgacgc ctcgaaggga accacgctgc cgatcgctct
     1261 cgccatcgtc gaagcgtacg tcctgcggcc ggagaaggct ccgtggtaca tcgatctcct
     1321 caacctgaac ctgaaccagc acgatcttgc agtcgcccga gaccggatca cccgcttctt
     1381 cgagacgttc gaacgcgacg gcatccggat caccgagaac ctggatttcg aatcctgagc
     1441 atacgaggag ctgttatgga acgattcgca cgcgaagtcg ccgccacgca ggagtggttc
     1501 ggcagccccc gcttcgaggg gataacccgc ctctacaccg cccgccaagt cgtcgaacag
     1561 cgcggtacca tcgagaccga ctacccggtg gcgcgggagg ccgccgccgc cttccacgcc
     1621 cgtctccgcg aactcttcgc ccagaagcgg agcatcacca ccttcgggcc ctactcgccc
     1681 ggtcaggcgg tggcgctcaa gcgtctcggg attgagggga tctaccttgg cggctgggcg
     1741 acctcggcga agggatcgac gaccgaagat cccggtcccg acctcgcgag ctatccgctc
     1801 tcgcaggttc ccgacgaggc agcggtgatc gtgcgcgcgc tgctgaccgc cgaccgcaac
     1861 cagcagtacc tgcgctcccg aatgagcgcc gagcagcggc agaaaacgcc ggtctacgac
     1921 taccgtccgt tcctcatcgc cgacgccgac accggccacg gcggtgaccc ccatgtgcgc
     1981 aacctgatcc gtcgtttcgt cgaggtcgga gtgaccggct atcacatcga agaccagcgt
     2041 ccgggaacga aaaaatgcgg ccaccaggga ggcaaggtgc tggtgccgtg cgacgagcag
     2101 atcaagcgtc tcaacaccgc ccgcttccag ctcgacatca tgaaggtgcc ggggctcatc
     2161 gtggcgcgca ccgacgcgga ggcggccaac ctcctcgacg gccgcggcga cgagcgcgac
     2221 cagccgttcc tgctcggggt cacgaacgtc tctctgccca cttacaagac cgcctggctc
     2281 gcgcttctcc gccgtttcca cgctcttggt gtcaccgcgc tcaacggtca tctgctctac
     2341 tcgctcggcg agccggagtg gcgctgggct gacgcctggc tggaacggac cgggatcgcg
     2401 caggccatcg ccaatgaggt ggccggcctt gacaccggcg acgcgctcgc cacggatgcc
     2461 gctttcgacc gggtagccaa ccgattcctc gacgcctggg agggcgaggc ccggctgcgg
     2521 acctacggcg aggcggtggc cgaggtgctg gccttccgcg agagcgaagg ggagaccctg
     2581 gagatggggg tcgacgcctg gcgtgccttc gccagcaccg cgtccctctg ggcggcacgg
     2641 gagcgggccc ggtcgctcgg cgtcagcgtg atctgggact gcgagcacgc caaaacgccg
     2701 gagggctact accagatccg tggcggcatc ccctacgcca tcgccaagtc actggcagtc
     2761 gctccgttcg ccgacctcct ctggatggag acgaagaccg cggatttggc cgatgcccgt
     2821 gagttcgccg aggcgatcca cgccgtctat ccggagaaga tgctcgccta caacctctca
     2881 ccatcgttca actgggactc gaccgggatg agcgaggacg agatgcgccg tttccccgag
     2941 gagctgggca agctcggctt cgttttcaac tttatcacct acggcggcca ccagatcgac
     3001 ggtgttgcag ccgaggagtt cgccacctcg ctgcgggagg atgggatgct cgcgcttgct
     3061 cgtctgcagc ggaagatccg cctcgtggag tccccctaca gaacgccgca gaccctcgtg
     3121 ggcggaccgc gctccgatgc cgcgctctct gcctgttcgg ggcggacggc gaccacgatg
     3181 gcgatgggga agggctcgac ccagcaccag cacctgatcc agaccgaggt gccgaagaag
     3241 cttctggccg agtggcttac gatctggaag gagtatcacg gtcttacctt gccgatcgcg
     3301 gtccaacttc tgcctcaccg cgccggctcc gagctgctcg aactcgcgct ggtgggcagc
     3361 aacggcggca agctcgccaa cgtcatcttc gccgcgatcc aggatcgccg ggagcggaac
     3421 atcctctccg tgcgcgacca gaacaccttc gatcccgagc tgcggcagaa gcgtctgatg
     3481 acgctcatcc agctttggct catccaccgc taccgggtcg attcggtgca ctacgtcacg
     3541 ccgaccgacg acaacgagca ccagaccgag aaaatgaagg agcaaggcct cttcaccgac
     3601 gtcaacaccg aggtcgggca gatcatcgtt gccgacgtca acgccacgcg ggtcgccgag
     3661 ctgctcgctc ccgaccgcgc tgccctcggc cggttgatcc gcaaggaggg gtgacgggtc
     3721 gtcgttgaca gtggggtctg ggctccctat tttttgtgga tttagagctg cgcccgccgt
     3781 gatctggcgg gcgctgggga gttccgggtc tcgtccacgg aatcagggcg gaccgatggt
     3841 gtgagcgcgg tgatagacgc accaggcaag ccagccatca tgcacgacac gtccgtgctc
     3901 ttggtcgcgg acggcttcag aagttgttgc cagtggccct tgccgtcgtt ggacctgggc
     3961 tccctcgtcg tcatcgtacc ggacgtcgcc atttgtcggc gtggcaaacc gcgtttgtgg
     4021 catgttgttt tggtaggggc accctttgcg ggtgcgctgg aatgtcgtag caatgtcgcc
     4081 atccaccgaa gtgaagtgca tctgagaagg ccgcgatgag caaagacgaa ttgacgatca
     4141 gccaggctgg gtatgccgat tggctggctg aactcgagct gcgcattcat gccgcccagc
     4201 atgcgccacg cttgcggcca gttgcgagct ggtgctgctg tactggccga ttggcccgga
     4261 cattctggcg cgccagacaa gtcagggcgg ggcagcaagg tggtggacag gcaggcccac
     4321 ggtctacgca atgcctggcc gaaggcatcg tcgcttggta caagcaactc gaccccgccg
     4381 accactccac ctgtgtgttg tgcgacagcg cttttaccaa tgacgtgacc aaaaccaata
     4441 gggccgtcat tctccaacaa cacggcattg ccaatgtgct aagccttttg ggagacaagc
     4501 gatgcaagcc acaagaatta ggttgaaaaa ctggcgtaac ttcaggacat tggatgcgcc
     4561 gatgcgtgac gtaacctaca ttctcggccc aaacgcttcc ggcaaatcca acttgctgga
     4621 tgtgttccgt ttcttgcggg atgtcagcaa accggccggt ggtggactgc aagcagcggt
     4681 gatcgcaagg ggcggaatca gcaagctacg ttgtcttcac gcacgtcggg acccagaggt
     4741 ttgcattgaa atggacctat cggatgctcc ggatgatgaa gtccccaatt ggcgttatgt
     4801 attgggattt agaccggagg gcaagggcgc tcaacgtatc ctggtgtcga aagaggaagt
     4861 atggcataaa ggcaagccat tgttttcgag accggataag gatgacatca aggattctgt
     4921 tctgctgacc caaacgcgcc ttgaacaaat tgctgccaat gcagagttca gggagcttgc
     4981 ggagttcttc ggctcaatca cctacctgca tctggtgccc cagcttttga agtatgcgga
     5041 tcagttgggt gggcggcctt tggacaatga cccctttggt cagagctttc tggagcggat
     5101 cgccaaaaca acggaacgtg tcagaaatac acgattgagg agaatcagtc aggcgttgac
     5161 attcgctgta ccgcaattca aggaacttcg gttcgtcaag gacgaatcag gtcaccctca
     5221 tctggaagcg ttgtattcac atcacagacc caacgcggga tggcaatcag aagagcactt
     5281 ttctgatgga accttgcggc tgctcgggct gttgtgggtt ctgctcgatg gcagttccac
     5341 gttgctaatg gaggagccgg aactctcact caatgatgcg gtagtgaaag agattccgct
     5401 tatcattcag cgcttgctaa agaacagaaa gcccaaacgg caagtattga tcagtaccca
     5461 cagccaggcg ctgctcagta accctggaat cgatggccgg ggcgttattc tgctggaagt
     5521 cacggcagat ggttcaaccg gccgaaccct gcaagaagat gaagcgttag cactcaaagc
     5581 agggctatct gtggccgagg tggtactgcc aaagacacgc cccagttctg tcaatcagct
     5641 cgagttttgg gaatgacatc ggttgcctta gccacggaag atgagctcag tgaagcggta
     5701 ggcgtaaagc tgttatcaga gcatcctgtt ttggcagaag ccccgccgat gttgctgaga
     5761 aaaaatggtt tggggtattt gcgctcacgc atggatagct ggcgacagat ggcccatcgc
     5821 cagattgtcg ttgttttgac cgatttggac aagctggcct gcccattgat cctgcttgcc
     5881 gactggttgg gtgctggtca gcaacaccct gataatctgc tgttgcgtat tgccgagcga
     5941 gaggttgaat catggctgct tgcggaccac gaagcgcttg gcaagctgct gggcagaaaa
     6001 tctcagtttc cgggccagcc ggacacgttg ccagatccca aacagcacct actccggctt
     6061 gcgcaaaaag cacccagagc agtacgagaa gacttggtcg ctcaacaagg cactgttgca
     6121 aggcagggga ttggctataa tcgccgcttg atggattggg tgcagaccga gtggtctccg
     6181 gaaagggctg ccgcccgttc ccctagcctt atgcgggcca gaaatgccat gcgtgagaca
     6241 gctcgtcgaa tcatgcaggc atagctatcc ttgtcctaca ccgaccatcc cacttgacgg
     6301 caataggacc ggacaccatg ggggcgtcct ttaacgtata cattgtcgct ttcgcgcaac
     6361 tgcctgc
//
LOCUS       DAOSIT010000060         6360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11071_length_6360_cov_9.498493, whole genome shotgun sequence.
ACCESSION   DAOSIT010000060 DAOSIT010000000
VERSION     DAOSIT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11071_length_6360_cov_9.498493"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="cheY"
                     /locus_tag="PLL14_02550"
     CDS             complement(<1..350)
                     /gene="cheY"
                     /locus_tag="PLL14_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HOG02829.1"
                     /translation="MADPKMRILVVDDFSTMRRIVRNLLKELGFSNVDEAEDGAIALQ
                     KLKNGTFEFVVTDWNMPNMDGLQLLKTIRGTPALKHLPVMMITAEAKKENIIAAAQAG
                     ASGYIVKPFTAATLA"
     gene            complement(372..1310)
                     /locus_tag="PLL14_02555"
     CDS             complement(372..1310)
                     /locus_tag="PLL14_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOG02830.1"
                     /translation="MELAERKNLLEGVDARTRLAGSNKMEILLFSLGTREIFGINVFK
                     VREVGRTPHITKTPNMPRGVEGLISLRGNVIPVLSLISFLEHKDQHHEHGKTMMVAEY
                     SKRTLGFLVHEVDRIIRVDWEKVKAPESVLSSNQGLITAITELDDGKLVSILDVEQIL
                     ANAFGEAIIVDIPPANIDEDVGIFFADDSIVARRKITEVLDKLGVKHKHATNGAEAWT
                     RLQGIAAHAAQTGRGVRDELRLILVDAEMPEMDGYVLTKNIKSDQRFSGIPVVMHSSL
                     SSQANHAMGKVVGVDAYVAKFDAEILADTLRPLLER"
     gene            complement(1337..2305)
                     /locus_tag="PLL14_02560"
     CDS             complement(1337..2305)
                     /locus_tag="PLL14_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOG02831.1"
                     /translation="MSELLKNIDARTKLAGTNKLEILLFSLGTDSRTGRRETFGINVF
                     KVREVMRTPPITSAPEMPASVEGMVSLRGALVPVVDLARYARVDTETPRSIMIVTEYA
                     GHTQGFLVEGVDTILRLDWSQMRVPPAMLLAEMGGLVTAVTELPDGRLVMMMDVEKIL
                     SETTNYDDEIVYRNIKPLDNPGLTVFFADDSTVARKQIQRTLEAMGVRYVAAINGLEA
                     WDELEKMAAYAQASGQEVTDLISLVLTDIEMPEMDGYILTKKIKSDPRFAGVPVIMHS
                     SLSGMSNQQLGKSVGVDEYVPKFEPQRLSETLTRRLQRALPAVQLT"
     gene            complement(2417..4114)
                     /locus_tag="PLL14_02565"
     CDS             complement(2417..4114)
                     /locus_tag="PLL14_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG02832.1"
                     /translation="MRGVAWTIRVAMLLQVLAAALAGWLGLGLFGGGVYGWLGALGGL
                     VVLALLIGLVVERRLLGRLEVLREVLAKTYADGDLTRRATVAGQDEIAAVAADFNRLI
                     GGFATIVGKVLFNSVEVGKASRQLIGDASRVASGSNQQRDAALATASAMGELTTNMHE
                     VSQNASETASISEASNSLSTEGMAIVRNASAEMEQIAASVTESARVVCALGERSRAIS
                     GIVQTIHEIADQTNLLALNAAIEAARAGDQGRGFAVVADEVRKLAERTSQATGEISRM
                     ITAIQGETQTAIASIEAGSGQARRGADLARQAAESLERINSGARETMEKVEAIAAAVV
                     QQSRTGESIAEHVRTIREMAEANSAVTAETLLAVEHVECLAENLGEVGNVFKLGPAGE
                     QAAAAHARMPATVQQAARAVGELFDKAVDSGRISVEDLFDDHYQPIPNTRPQKFKTRF
                     DAFTDQNVVELQENLLHQNKCLIYAICIDRNGYVPTHNRKFSQPLTGDEKTDFVNSRG
                     KRIFDDPVGKRCGSHQQPFLLQTYRRDTGEVLHDISAPIFVKGRRWGGFRIGYATEF"
     gene            complement(4227..6227)
                     /locus_tag="PLL14_02570"
     CDS             complement(4227..6227)
                     /locus_tag="PLL14_02570"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG02833.1"
                     /translation="MRRLAPLDLWQWLGIALLGVLLAHASGHIELGSLNRIDALLYDA
                     RVRLLARAEPDPRIAIVDIDERSLAELGRWPWNRARLADLVDSIFRQHGALLLGLDVI
                     LAEPDESSGLASLDTLGHGRLRHDDAFQAALADLRSQLDYDGRLAAVFRRYPVVLGFH
                     LSSGADAASSGELPPPLLPGAGEQAGQLTRWTGYGANLPRLQQAAGAAGFLNAPIDPD
                     GVTRRALLLARHDGKMYAALSLVMAQILLGNPPLQLRFAEPAPGSTGNGTLEAIELLT
                     ARGVLRIAVDAQAGVLLPFRGPMGSFAYHSAADVLAGRVPADSLRGRVVLLGTTAPGL
                     LDQRVTPVGEAYPGVEAHANLLSGLLAGDLPQSPTYSPGVEVLGLLFVGTALLFGLPR
                     FSPAGAVLLAMSLLLVVVASNLAAWAFAHLVLPMAASLLLVAMLLALHLFFGYFVEHR
                     AKRRLTELFGQYVPPELVEEMNRDPTHYSMEGRSSEMTVLFADVRGFTTLAEGMPPDE
                     LARLMNDYFSAMTDVIRAHRGTLDKYVGDALVAFWGAPLADAQHARHAVEAGLAMQVA
                     LIGVNQRFASRAWPSLRIGIGINTGTMVVGDMGSRHRRSYTVLGDAVNLASRLEGLSA
                     QYGAGVIIGEATFQALGEWRCRQLDRVTVRGRTTPVAIYEPL"
     gene            complement(6244..>6360)
                     /locus_tag="PLL14_02575"
     CDS             complement(6244..>6360)
                     /locus_tag="PLL14_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOG02834.1"
                     /translation="GVPRDAISVAGRGEREPVVVTADEVAEPRNRRVDIKLR"
BASE COUNT         1235 a         2125 c         1807 g         1193 t
ORIGIN      
        1 gccagtgtcg ccgcggtgaa gggcttgaca atgtagccgc tggctcccgc ttgcgcggcg
       61 gcaatgatgt tttccttctt tgcctcggcg gtgatcatca tcaccggcag gtgcttgagg
      121 gccggcgtgc cacgaatcgt cttcaagagc tgcaggccat ccatgttcgg catgttccag
      181 tcggtgacga caaactcgaa cgtgccattt ttcagcttct ggagtgcgat cgccccatcc
      241 tcagcctcat ctacattaga gaaccccagt tccttcagga gattcctgac aatgcgtcgc
      301 attgtcgaaa aatcgtccac taccagaatt ctcatcttcg gatcagccat tctctactcc
      361 tgtgggttct ctcagcgctc gagcaagggg cgcagcgtgt cggcaagaat ctcggcgtcg
      421 aacttggcaa cataggcatc cacgcccacc accttgccca tggcgtggtt ggcctgggac
      481 gacagggatg aatgcatgac caccggaatt cccgagaaac gctggtcgct cttgatgttc
      541 ttggtcagca catagccgtc catttcaggc atttcggcat cgaccaggat caggcgcagc
      601 tcgtcgcgaa caccccttcc tgtctgcgct gcgtgcgccg cgattccctg caggcgggtc
      661 caggcctctg ctccgttcgt agcgtgcttg tgtttgacgc cgagtttgtc gagtacctcg
      721 gtaatcttcc gccgggcaac aatcgaatca tcagcaaaaa atatgcccac atcctcatcg
      781 atgttggccg ggggaatatc gacgatgatc gcctcgccaa aggcattggc gagaatctgc
      841 tcgacatcga gaatcgacac cagtttgccg tcatcgagtt cggtgattgc cgtgatcagt
      901 ccctgattgc tggacaacac actttcggga gccttcacct tctcccagtc gacacgtatg
      961 atccgatcca cctcatgcac cagaaacccc agtgtccgct tggagtactc ggcgaccatc
     1021 atggtcttgc cgtgctcgtg gtgttgatcc ttgtgttcaa gaaaggagat cagagacaac
     1081 accggtatga cgttgccgcg cagtgaaatc aggccttcga cgccgcgtgg catgttcggc
     1141 gtcttggtga tgtgtggggt ccgaccgact tcgcgcacct tgaagacgtt gattccaaaa
     1201 atctcgcgcg tgcctagaga gaacagcaga atctccatct tgttggaacc ggcgagccgg
     1261 gttcgcgcgt caacgccctc gagcaggttt ttcctttcag ccaactccat tgtatcgctc
     1321 cagaaaaatc gcgaaatcag gtcaactgca ctgccggaag agcccgctgc aaccgtcgtg
     1381 tcagcgtttc cgagaggcgc tgtggttcga atttgggaac atactcatcg acaccgacgg
     1441 acttgcccaa ctgctggttc gacatccccg acagtgagga atgcatgatc accggcacgc
     1501 cggcgaagcg agggtcagac ttgatcttct tggtcaggat ataaccatcc atctcgggca
     1561 tttcgatgtc ggtcaatacg agactgatca ggtcggtcac ttcctggccg gaagcctgcg
     1621 cgtaggcggc catcttctcc agttcatccc aggcttccag gccattgatc gcagccacgt
     1681 agcgcacgcc catcgcctca agggtccgtt ggatctgctt gcgcgccacc gtcgagtcgt
     1741 ctgcaaaaaa tacggtcagg ccgggattgt cgagcggctt gatattccgg tagacgatct
     1801 cgtcgtcgta gttcgtcgtt tccgagagaa tcttctcgac gtccatcatc atcaccagac
     1861 ggccgtccgg caactcggtc accgccgtca ccagaccgcc catctcggcc agcagcatgg
     1921 cgggaggaac ccgcatctga ctccagtcca gacggagaat ggtatccacc ccctcgacaa
     1981 gaaaaccctg cgtatgaccg gcgtactcgg tgacgatcat gatcgagcgc ggtgtctccg
     2041 tatcaacccg ggcatagcgg gccagatcaa cgaccggcac cagcgccccc cgcaaactga
     2101 ccattccctc gaccgacgcg ggcatttccg gcgccgaggt aatcggcggc gtccgcatca
     2161 cctcgcgcac cttgaagaca ttgatgccga aagtctcgcg tcgccccgtg cgcgaatcgg
     2221 tccccagcga gaacaggagg atctccagct tgttggttcc ggccagcttg gtgcgggcat
     2281 caatgttttt cagcaactct gacatttttc tcttcctcgc gcaaagaaca gcccacgcct
     2341 tatccccgac atcttaatcc aatacgtggc gccgcgtcat gatcgtccac tacaacacga
     2401 gcatcagcag gccggcttag aactcggtcg catagccgat gcgaaagcca ccccagcgcc
     2461 gacccttgac gaaaatgggc gccgagatgt cgtgcaggac ttcaccggta tcgcggcgat
     2521 aggtctgcag caggaacggc tgttggtgac tgccacaacg cttgcccacc gggtcgtcga
     2581 agattcgctt cccacggctg ttcacgaaat cggtcttttc gtctccggtc agcggttgcg
     2641 agaatttccg gttgtgggtg ggcacgtaac cgttgcggtc gatgcatatg gcgtagatca
     2701 gacacttgtt ctgatgcagc agattctcct gcagttcgac cacgttctgg tcggtaaagg
     2761 cgtcgaaacg ggttttgaac ttctgcggcc gcgtgttcgg aatcggctgg tagtggtcat
     2821 cgaacaggtc ctccacgctg atcctgcctg aatcaactgc cttgtcgaag agttcgccga
     2881 cagcacgcgc tgcctgctgg acggttgccg gcattctggc gtgtgcggcg gccgcctgct
     2941 cacctgccgg gccgagtttg aaaacattgc cgacctcccc gaggttctcg gccagacact
     3001 ccacatgctc gaccgccagc aacgtctccg cagtgaccgc gctgttggct tcggccattt
     3061 ccctgatcgt tcgtacgtgc tcggcaatac tctccccggt acggctctgc tggaccaccg
     3121 cggcagcgat cgcttccacc ttctccatcg tctcacgcgc gccgctgttg atccgctcca
     3181 gcgactcggc cgcctgccgg gcgaggtccg cccccctgcg cgcctgccca ctgccggcct
     3241 cgatgctggc aatcgcggtc tgcgtctcgc cctggatcgc cgtgatcatc ctgctgatct
     3301 cgccggtcgc ctgcgaggtc cgttcggcaa gcttgcgcac ctcatcggcg accaccgcaa
     3361 aaccccgtcc ctggtcaccg gcacgcgccg cctcgatcgc cgcattgagg gccagcagat
     3421 tggtctggtc ggcaatctcg tggatcgtct ggacgatccc gctgatcgcc ctggaacgtt
     3481 cgccgagcgc acacaccacc cgcgcggact cggtcaccga agcggcgatc tgttccatct
     3541 ctgccgaagc attacgtacg atcgccattc cttcggtcga caagctgttc gaggcctctg
     3601 aaatgctcgc ggtttcgctg gcgttctgac tgacctcgtg catgttggtg gtcaactcac
     3661 ccatggcact ggctgtcgcc aacgcagcgt cacgctgctg attcgacccc gatgccaccc
     3721 ggctggcgtc accgatcagt tgccgggagg ccttgccgac ctcgaccgaa ttgaagagga
     3781 ccttaccgac aatggtcgcg aatccgccaa tcaagcgatt gaaatcggcc gccacggcgg
     3841 caatttcatc ctgccccgca acggttgcgc gcctggtcag atcaccatcg gcgtaggtct
     3901 tggcgagcac ctcgcgcagc acctcaagac gacccaacag acgccgctcg accaccagac
     3961 cgatcagcag ggcaagcacc accagccccc ccaaggcgcc aagccagccg tagactcccc
     4021 caccgaacag cccaaggccc aaccagccgg ccaatgctgc ggcaagcacc tgcagcagca
     4081 tcgcgacgcg tatcgtccac gcaactcccc tcatcctcgt ctccttatcg ttatatgcat
     4141 ccattttatt cactacgtta cggcatcttt tgccgcgcct tgagcttccg cctggcgcaa
     4201 ctcgaccact ggcctcgtca gttcaatcac agcggctcgt agatcgccac cggcgtggtg
     4261 cgcccgcgca ccgttacgcg gtcgagctgc cggcagcgcc actcgccgag agcctggaac
     4321 gtcgcctcgc cgatgataac gcccgcaccg tattgcgccg acaagccttc gaggcgggaa
     4381 gccaggttca ccgcatcgcc gagcacggtg taggagcgac gatggcgcga acccatgtcg
     4441 ccgacaacca tcgtacccgt gttgatgccg atgccaatcc gcagcgacgg ccaggcgcgg
     4501 ctcgcaaagc gctgattgac gccgatcagc gcgacctgca tggccaggcc ggcctcgacc
     4561 gcatgccggg cgtgttgtgc gtcggccagc ggcgcacccc agaaagccac cagcgcgtcg
     4621 ccgacatact tgtccagagt cccgcggtgc gcgcggatga catcggtcat cgccgagaag
     4681 taatcgttca tcagccgcgc cagttcgtcg ggcggcatcc cctcggccag ggtcgtgaaa
     4741 ccacgtacgt cggcgaacag caccgtcatc tcggaactgc gtccttccat gctgtaatgc
     4801 gtgggatcac ggttcatttc ctcgaccagt tcgggtggga cgtactggcc aaacagctcg
     4861 gtcagacgtc gcttggcacg atgttccacg aaatagccga agaacagatg cagggccagc
     4921 agcatcgcaa ccaacagaag actggccgcc atcggcagca ccaggtgtgc gaaggcccag
     4981 gcggccagat tgctggccac caccaccaac aacaacgaca tcgccagcag caccgccccc
     5041 gcaggtgaaa acctcggcag gccgaacagc agcgcggtgc cgacaaacag caagcccagg
     5101 acctcgacgc ccggcgaata ggtgggcgac tgtggcaggt ctcctgccag caggccggac
     5161 aacaggttcg cgtgcgcttc gacaccggga taggcctcgc cgaccggtgt gacgcgctgg
     5221 tcaagcagac ccggcgcggt cgttccgagg agcaccaccc ggccacgcag gctgtcggcc
     5281 ggcacgcggc cggccagcac atcggccgcc gaatggtaag caaaactccc catcggtccc
     5341 cgaaagggca gcagcacgcc cgcctgcgca tcaaccgcga tgcgcagcac tccgcgcgcc
     5401 gtcaataatt caatcgcctc cagtgtgccg ttcccggtcg accccggagc tggttcggca
     5461 aagcgcagtt gcagaggtgg attgcctagc aggatctgcg ccatcaccaa cgacagggct
     5521 gcgtacatct tgccgtcatg ccgcgccagc aacagcgcac gccgcgtcac gccgtcgggg
     5581 tcgattggcg cattgagaaa accggcggca ccggcagcct gctgtaaccg tggcaggttg
     5641 gctccataac ccgtccaccg cgtcagttgc ccagcctgct caccagcgcc cggcaacagc
     5701 gggggcggca attccccgct gctggccgca tccgcaccac tcgacagatg aaacccgagc
     5761 acgaccgggt agcggcggaa caccgcggcc agacgaccgt cgtaatccag ttgcgatcgc
     5821 aagtctgcca gtgcggcctg gaaggcatcg tcgtggcgta gtcggccgtg cccaagggta
     5881 tccagcgaag ccagccccga actctcgtcc ggttcggcca ggatcacgtc cagaccgagc
     5941 agcagcgcac cgtgttgtcg aaaaatgctg tcgaccagat cggccaggcg tgcccgattc
     6001 cacggccagc gtcccagttc ggccagcgaa cgctcgtcga tgtcgacgat ggcgatgcgg
     6061 gggtcgggtt ccgcacgcgc caacaaacgc acgcgggcgt cgtaaagcag tgcgtcgatt
     6121 cggttcaggg aaccgagctc gatatggcca gaagcgtgcg caagcagcac gccaagcaag
     6181 gcaatgccca gccactgcca caggtcgagc ggcgccagac ggcgcaagag cgtatgcgcc
     6241 agatcaacgg agcttgatgt cgacacggcg gttacgcggc tcggccacct cgtcggcggt
     6301 gaccaccacc ggttcgcgtt cacctcgccc ggccacggag atggcatcgc gcggcacccc
//
LOCUS       DAOSIT010000061         6323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11153_length_6323_cov_9.913050, whole genome shotgun sequence.
ACCESSION   DAOSIT010000061 DAOSIT010000000
VERSION     DAOSIT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11153_length_6323_cov_9.913050"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..360
                     /locus_tag="PLL14_02580"
     CDS             4..360
                     /locus_tag="PLL14_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG02835.1"
                     /translation="MKTSYRDIVVYTTRDGSAIRELMHPACHGNERQSLAEAVIRPGE
                     QTRLHRHQCSEELYHVTRGTGIMTLAGETLCIGVGDTILIPPRTPHCVAATGNEPLHI
                     LCCCSPPYRHEDTELL"
     gene            463..>1115
                     /locus_tag="PLL14_02585"
     CDS             463..>1115
                     /locus_tag="PLL14_02585"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG02836.1"
                     /translation="MIKFVDPSRYHSMKATGKLPSPKGVAFSIIKLLQKDDFRVSDLV
                     QLVQSDPAIAGRLLKFANAAAFGRARPIVSLQRAIVALGSFRVRDLVIGLSVMHSHTS
                     GQCPAFDYGAFWGHSLATGIACQELAHFAQISSEELFTIGLLARVGELAMASLYPEEY
                     AVVLLKARETQQDLAALERQRFDMDHNQLGATMLGEWGLPEMLIQAAYHHEQPDLAG"
     assembly_gap    1116..1215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1216..2238
                     /locus_tag="PLL14_02590"
     CDS             <1216..2238
                     /locus_tag="PLL14_02590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG02837.1"
                     /translation="ILAASPPSRRVSNVSPEQNGKDAAPRLRVQLLGIPLAELPALIR
                     QVESLGHQPVLVDSTPEGLKQALREPAQIVIADMAMPGLKPAAFCRILRQTPAGKETY
                     ALLLASPEGEEHILEAIDAGADDVLVKPLNIQTLRVRLNTATRMLRLREEIQRERRGI
                     MRSTDEFAVAHKRLLQEALTDPLTQLPNRRHGLDFLASEWAFAQSNALPMACLLLDID
                     HFKRINDTYGHAAGDAVLRQLAELLKRASRAEDLVFRYGGEEFAAVLPNATARAAVQI
                     AERIRSVVEKYSFLWEGQTIPVTLSIGVASLHGAEMDSQALIQASDAALFEAKRNGRN
                     RVAVAA"
     gene            complement(2244..3023)
                     /locus_tag="PLL14_02595"
     CDS             complement(2244..3023)
                     /locus_tag="PLL14_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOG02838.1"
                     /translation="MNRQRRRATLTLLALPAIGATGGMAGLAAAVASDGDDPRRIDLP
                     RFSAAPPGHRLPAGWAHQTLPSVERPNRFDLVADEGVTVLRVLSDHSASSLMVVQRVD
                     PAATPLLRWRWRVSNSVAASDLRRKAGDDYAGRVYVFFDLPPERLRVADRLKIAAARL
                     LHGADLPAAALCYVWGTAQAAGESGWNAYTDRLRMLVVDSGNGHAGQWREVVRDVAAD
                     FRAAFGAPVPAISGVAVAADTDNSGERVEARFGDLWFEARP"
     gene            complement(3020..3667)
                     /locus_tag="PLL14_02600"
     CDS             complement(3020..3667)
                     /locus_tag="PLL14_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOG02839.1"
                     /translation="MLPGNTRPPGIALFARAPQAGAAKTRLIPALGAAGAARLQRRLT
                     LQALATAQRAALGRVTLWATPDTGQRFFRAVQRRCDIDLRSQPAGDLGARMAAAFAAQ
                     AGPLLLIGCDCPVLRAADLVMAAQALRHAGPDGHDAVFIPVDDGGYMLVGLRCPQPRL
                     FEDIDWGSERVMAQTRLRLSALGLRWHELPSLWDLDRPEDLVRLATLPGFAAWQR"
     gene            complement(3648..4367)
                     /locus_tag="PLL14_02605"
     CDS             complement(3648..4367)
                     /locus_tag="PLL14_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOG02840.1"
                     /translation="MPNRDTATERPLLSLILPVLDEAATLPAQLARLQDLRRRGAEVL
                     VVDGGSADATPILAQPLVDRLIAAPRGRATQMNAGAGAARGQVLLFLHADTTLPPAAD
                     ALIHAAIAAGAAWGHFDVRIDARHPLLRLVERMMNWRSRLSGIATGDQAIFVRHDIFT
                     RLGGFPELPLMEDIAFSKLLKRIARPACLRAQVLTSGRRWEKHGVLRTIVLMWRLRAS
                     YFLGADPRRLAIRYGYAPRQH"
     gene            complement(4388..5194)
                     /gene="dapB"
                     /locus_tag="PLL14_02610"
     CDS             complement(4388..5194)
                     /gene="dapB"
                     /locus_tag="PLL14_02610"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOG02841.1"
                     /translation="MNGMRIAITGAGGRMGRMLIEATLHDTEATLVAALDQPGVPAEG
                     KDAGELVGMPCGVPVSSDCEAGIALANCLIDFTRPEATLEHLAICRRHGVAMVIGTTG
                     FDAAGKQAIAAAAQDIPVVFAPNMSVGVNVVFRLLDVAARILDQGYDVEIVEAHHRHK
                     VDAPSGTALRMGEVVAQALGRDLKACAIYGREGVTGERLQSTIGFATVRGGDIVGDHT
                     VMFCGQGERVEIGHKASSRMPYALGSLRAARFLADRACGLFDMQDVLGLR"
     gene            complement(5196..5678)
                     /gene="bamE"
                     /locus_tag="PLL14_02615"
     CDS             complement(5196..5678)
                     /gene="bamE"
                     /locus_tag="PLL14_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOG02842.1"
                     /translation="MIFPRFAPVVIAFGLLSACSSVPRIVTEYRIDVQQGNVLTQEMV
                     SQLRPGLTRDQVRFILGTPVLMDMFHANRWDYFYWLKKGSSGEVDTRRFAVFFDADGK
                     LISVAGDVAAAQPSDSTVVSETRNREIDLGSLPTDATVTMPPPEERGFFRSMIDIVGF
                     "
     gene            5857..>6323
                     /gene="fur"
                     /locus_tag="PLL14_02620"
     CDS             5857..>6323
                     /gene="fur"
                     /locus_tag="PLL14_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HOG02843.1"
                     /translation="MSNSEDLKSMGLKATIPRLKILALFERTEVRHLTAEDVYRLLLA
                     ENLDIGLATVYRVLTQFEQAGLLERHYFESGKAVFELSPGQHHDHLVCIDCGRVEEFY
                     DAEIERRQTRIAKERGFVIREHALYLYAECTKTDCPHRGSPARNIAASMSKARP"
BASE COUNT         1141 a         2179 c         1957 g          946 t
ORIGIN      
        1 cccatgaaaa ccagttaccg ggatatcgtc gtctacacca cccgcgatgg ctcggcgata
       61 cgcgagttga tgcatcccgc gtgccatggc aatgaacgac aaagcctcgc cgaagcggtc
      121 atccgccccg gcgaacagac gcgtctgcat cgccaccagt gcagcgagga gttgtaccac
      181 gtcactcgcg gcaccggcat catgacgctt gccggcgaga cgctgtgcat cggcgtcggc
      241 gacaccatcc tgattccgcc gcggacgccg cattgcgtcg ccgccaccgg caacgagccg
      301 ctgcacattc tctgctgctg cagcccgccc taccgccatg aagataccga actgctttga
      361 ccggccggcg cgccggcgca gaacgatcgg cgacaacggc gccggacagg aggcaccttt
      421 tttgtagcat aatcggccgc aaatgttgaa gtcgagactt ccttgatcaa atttgtcgac
      481 cccagccgct atcactcgat gaaggcgaca ggcaaactgc cgtcaccgaa gggagtcgcc
      541 ttttcaatca tcaagctgct gcagaaggat gacttccggg tttccgatct ggtacaactg
      601 gtccagtcag acccggcgat tgccggtcgc ctgctcaagt ttgccaatgc cgcggctttc
      661 gggcgggcgc ggccgatcgt ctcgctgcag cgggcgatcg tcgccctcgg gtcgttccgg
      721 gttcgcgacc tggtgatcgg cctctcggta atgcacagcc acaccagcgg acaatgcccg
      781 gcctttgact atggggcttt ctgggggcat tcgctggcca ccggcattgc ctgccaggaa
      841 ctcgcccact ttgcgcagat ctccagcgag gagctgttca ccatcggcct gctggcgcgt
      901 gtcggcgaac tcgccatggc ttccctctac cccgaggagt acgccgtggt actgctcaag
      961 gccagggaaa cgcaacagga cctcgccgct ctcgaacgcc agcgtttcga catggaccac
     1021 aatcagctcg gggcgacgat gctcggcgaa tggggcttgc cggagatgct gattcaggcc
     1081 gcctaccatc atgagcaacc cgacctcgcc ggtttnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnatcct ggcggccagc ccgccgagcc gccgcgtcag caacgtcagc
     1261 ccggaacaga acggcaagga cgccgcgccg cgcctgcgcg tgcagttgct cggcattccg
     1321 ctggcggagt tgcccgccct gatccggcag gtcgaaagcc tgggccacca gccggtcctc
     1381 gtggacagca cgcccgaggg cctgaagcag gccttgcgcg aaccggccca gatcgtgatt
     1441 gccgacatgg ccatgccggg actgaaaccg gccgctttct gccgaatcct tcgccagacc
     1501 ccggcgggca aggaaaccta cgccctgctc ctggcttccc cggaaggcga ggagcacatc
     1561 cttgaggcca tcgatgccgg cgccgatgac gtactggtca aaccgctcaa catccagacc
     1621 ctgcgcgtcc ggctgaacac cgccacccgc atgctgcgtc tgcgcgaaga gatccagcgc
     1681 gaacgacgcg gcatcatgcg ctcaaccgac gagtttgccg tcgcccacaa acgtctcctg
     1741 caggaagcgc tgaccgaccc cctgacgcaa ctgcccaacc gccgccacgg gctcgacttc
     1801 ctggcctcgg agtgggcctt cgcgcagtcc aacgccttgc cgatggcctg cctgctgctc
     1861 gacatcgacc atttcaagcg catcaatgac acctacggcc atgccgccgg cgacgcggtg
     1921 ctgcgccagc tcgcggagtt gctcaagcgc gcctcgcgcg ccgaagacct ggtgtttcgc
     1981 tacggcggcg aggagttcgc cgccgtattg ccgaatgcca cggcgcgcgc cgccgtgcag
     2041 atcgccgagc ggatccgttc ggtggtcgag aaatacagct ttctctggga aggtcagacg
     2101 attccggtca ccttgagtat cggcgtggcc agcctgcacg gtgccgaaat ggacagccag
     2161 gcgctgatcc aggcgtccga tgcggcgctc ttcgaggcca agcgaaacgg tcgcaaccgg
     2221 gtcgccgtcg ccgcctgaca gcttcagggc cgcgcctcga accacaggtc gccgaagcgc
     2281 gcctcgacac gctcgccgct gttgtcggta tcggccgcga cggcaacacc ggaaatggcc
     2341 ggcaccggcg caccgaaagc ggcacggaaa tcggcggcga catcgcgaac gacttcgcgc
     2401 cactgtccgg catgcccgtt cccggaatcg acgaccagca tgcgcaggcg atcggtatac
     2461 gcattccacc ccgactcgcc ggccgcctgc gccgtccccc agacgtagca cagcgccgcc
     2521 gccggcagat cagcgccatg cagcagccgc gccgcggcaa tcttcaatcg atcggccaca
     2581 cgcagacgtt cgggcggcag gtcgaagaac acatagacgc ggccggcgta gtcatcgccg
     2641 gccttgcgcc gcagatccga tgcggcgacg ctgttcgaca ctcgccaacg ccagcgtagc
     2701 aacggcgtcg ccgccggatc gacacgctgc acgaccatca ggctggacgc cgaatgatcg
     2761 gacaacaccc gcagcaccgt taccccctcg tcggccacca ggtcaaagcg gttcggccgc
     2821 tcaaccgaag gcagcgtctg atgcgcccat cccgcaggca ggcgatggcc cggtggcgcc
     2881 gcggagaatc gcggcagatc gatgcgccgt gggtcgtcgc catcgctcgc caccgccgcg
     2941 gccaacccgg ccatgccccc cgtggcgccg atcgcgggca acgccagcag ggtcagcgtc
     3001 gcccgccggc gctgcctgtt catcgctgcc acgcggcgaa ccccggcaac gtcgccagac
     3061 gcaccagatc ctccggacga tcgagatccc acaagctggg caattcatgc cagcgcaggc
     3121 ccagcgcgga cagccgcagc cgcgtctgcg ccatcacccg ctcgctgccc cagtcgatgt
     3181 cctcgaacaa ccgcggctgc gggcagcgca ggccgaccag catgtagccg ccatcgtcga
     3241 cgggaatgaa taccgcgtcg tggccatccg gccccgcgtg ccgcagcgcc tgcgccgcca
     3301 tgaccagatc cgcggcgcgc agaaccggac agtcgcagcc gatcagcagc aacgggccgg
     3361 cctgcgccgc gaaggcggcg gccatgcggg caccgaggtc gcccgccggc tgactgcgca
     3421 gatcgatgtc gcagcggcgc tgcacggcgc ggaagaagcg ctgtcccgtg tccggcgttg
     3481 cccacagggt gaccctgccc agcgccgcgc gctgtgccgt ggccagcgcc tgcagtgtca
     3541 gacggcgctg caggcgggcg gcgccggccg caccgagcgc cggaatcagg cgcgtcttgg
     3601 ccgcaccagc ctgcggtgcg cgcgcaaaca gggcgatgcc cggcggtcta gtgttgccgg
     3661 ggagcatagc cataacggat ggccagccgc cgcgggtcgg cgccaagaaa ataactcgcg
     3721 cgcagacgcc acatcaacac gatggtgcgt aacaccccgt gcttctccca gcgccgcccg
     3781 gaagtgagca cctgcgcgcg caggcaggca ggccgcgcaa tgcgcttcag cagtttggag
     3841 aaggcgatgt cctccatcag tggtagctcg ggaaagccgc ccaggcgcgt aaagatgtcg
     3901 tggcgaacga agatcgcctg gtcgccggtg gcaatgccgc tcagccgcga gcgccagttc
     3961 atcatccgtt ccactaggcg taacagggga tggcgggcgt cgatacgcac gtcgaagtgg
     4021 ccccacgccg ctcccgccgc gatcgccgcg tggatcagcg catcggcggc cggcggcagc
     4081 gtcgtgtcgg cgtgcaggaa gagcagcacc tgcccacgcg cggcaccggc gccggcgttc
     4141 atctgcgtgg cgcggccgcg cggcgcggcg atcagccggt cgaccagcgg ctgcgccagg
     4201 atcggcgtcg cgtcggcgct gccgccatcg accaccagta cttccgcacc ccggcggcgc
     4261 aagtcctgca agcgggccaa ctgagccgga agtgtcgctg cttcgtcgag caccggcagt
     4321 atcagcgaca gcaggggacg ctcggtcgca gtgtccctgt tcggcacagg agtctctcgc
     4381 gtgcggatca gcgcagtccg agcacgtcct gcatgtcgaa cagtccgcac gcccggtcgg
     4441 ccaggaaacg cgcggcgcgc aggctgccca gcgcgtaggg catgcggctg ctggccttgt
     4501 ggccgatttc gacgcgctcg ccctgaccac agaacatgac cgtatggtcg ccgacgatat
     4561 cgccgccgcg aacggtggcg aaaccgatgg tcgactgcag gcgctcgccg gtcacgcctt
     4621 cacggccgta gattgcgcac gccttgaggt cacgaccgag cgcctgcgcc accacttcgc
     4681 ccatgcgcag cgccgtcccc gagggggcgt cgaccttgtg ccggtgatgg gcctcgacga
     4741 tctcgacgtc gtagccctgg tcaagaatcc gcgccgccac gtcgagcagc ctgaaaacaa
     4801 cgttgacccc gacgctcatg ttcggagcga agaccaccgg gatgtcctgc gccgcagccg
     4861 caatcgcctg cttgccggcg gcgtcgaaac cggtggtgcc gatgaccatc gccaccccat
     4921 gccggcgaca gatggcgagg tgctcgagcg tcgcttcggg acgcgtgaaa tcgatcaggc
     4981 agttggccag cgcgatgccc gcttcacaat cgctgctgac cggcacgccg caaggcatgc
     5041 cgaccagttc gccggcgtcc ttgccctcgg ccggtacgcc cggctggtcg agagcagcca
     5101 ccagtgtcgc ttcggtatcg tgcagcgtcg cctcgatgag cattcgcccc atgcgaccac
     5161 cagcgccggt aatcgcgatt cgcatcccat tcatttcaga atcctacgat atcgatcatg
     5221 ctgcgaaaga aaccccgctc ctcgggaggc ggcatcgtga ccgtcgcatc ggtgggcaac
     5281 gaaccgagat cgatttcccg gttgcgggtt tccgagacga ccgtcgagtc tgacggctgc
     5341 gcggccgcga catcaccggc cacgctgatc agcttgccgt cggcatcgaa gaagaccgca
     5401 aagcgacggg tgtcgacctc tccggaactc cccttcttca gccagtagaa gtaatcccag
     5461 cggttggcgt gaaacatgtc catcaacacc ggcgtgccga gaatgaagcg cacctgatcc
     5521 ctggtcaggc cggggcgcaa ctgcgaaacc atctcctgcg tcaggacgtt gccttgctgc
     5581 acgtcgatcc ggtactcggt cacgatacgc ggcaccgacg agcaggcgga gagcagcccg
     5641 aaggcgatga cgacaggggc aaagcgcggg aagatcatgt tctggtcgga tggtgtcggg
     5701 gaatcaatag ccggcgggca gtggccagga agcttctcga ccgcggccga agcatgggtc
     5761 gagccgttcg aaaactggtg tcagtgcctc acaagcggcg gcaagggtat atcataactg
     5821 aaaaccctcc cttcgcagcc gaacaaggta agcccgatga gcaactccga agacctcaag
     5881 agcatggggc tgaaagccac cattccgcgc ctcaagatcc tcgctctctt cgagaggacc
     5941 gaggtgcgcc atcttacggc cgaggatgtc taccggctgc tgctcgccga aaacctggac
     6001 atcggcctgg ccaccgtcta ccgcgtgctg acccagttcg agcaggccgg cttgcttgag
     6061 cgccactact tcgagtccgg caaggcggtg ttcgagttga gccccggcca gcaccacgat
     6121 cacctggtat gtatcgactg cggtcgcgtc gaggaattct acgatgccga gatcgagcgc
     6181 cgacaaacgc ggattgccaa ggagcgcggc ttcgtgattc gcgaacacgc cctctatctc
     6241 tatgccgaat gcaccaaaac cgactgcccg catcgcggca gtccggccag gaacatcgcg
     6301 gcgagcatga gcaaagcgcg tcc
//
LOCUS       DAOSIT010000062         6246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11331_length_6246_cov_11.519948, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000062 DAOSIT010000000
VERSION     DAOSIT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11331_length_6246_cov_11.519948"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..301)
                     /locus_tag="PLL14_02625"
     CDS             complement(86..301)
                     /locus_tag="PLL14_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOG02844.1"
                     /translation="MVVSPCINVCRMDEQTGLCQGCFRTIEEIAVWSRASDAQRLRVL
                     LAVERRRVEHDPLGCAAGGDFRGDCER"
     gene            complement(363..1289)
                     /locus_tag="PLL14_02630"
     CDS             complement(363..1289)
                     /locus_tag="PLL14_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOG02845.1"
                     /translation="MSLPQRVKLVEVGPRDGLQNERQLVPAAIKIELIERLAAAGLSA
                     IEATSFVSPKWVPQMADNAEVLRAVLASPRRRSQVSYPVLTPNLKGFDAALEAGALEV
                     AIFAAASETFSQKNINCSIAESLKRFAAVLDAASALEIPVRGYVSCGVACPYEGAVDP
                     AKTARVARQLFEMGCYEVSLGDTIGAGTPASVQRMIEACAALLPIDRLAGHYHDTYGM
                     AIANIYASLELGMAVFDSSIAGLGGCPYAAGASGNVATEDVVYLLQGLDIETGVDMEQ
                     LLAAGQFIAGHLGREPASKAARALLARCGAGC"
     gene            complement(1286..3331)
                     /locus_tag="PLL14_02635"
     CDS             complement(1286..3331)
                     /locus_tag="PLL14_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HOG02846.1"
                     /translation="MFTKILIANRGEIACRVIRTARRMGVRTVAVYSEADAEARHVRV
                     ADEAVCIGPAAARASYLCGERILDAAARTGAQAVHPGYGFLSENDRFADACDAAGIVF
                     IGPPAAAIRAMGSKSAAKTLMAAAGVPLTPGYHGDDQEPAFLQRQAEAIGYPVLIKAA
                     AGGGGKGMRLVERGEDFLAALASCQREAISAFGSDQVLIEKYLRRSRHIEIQIFADTQ
                     GNCVHLFERDCSVQRRHQKVLEEAPAPGMTPARRALIGQAAVEAARAVGYVGAGTVEF
                     IAGDSFAQDGRFYFMEMNTRLQVEHPVTEMITGQDLVEWQLRVAGGEPLPLRQEQLDI
                     RGHALEARIYAENPEHDFLPSTGRLLHLARPPESLNVRVDTGIEQGDEITPYYDPMIA
                     KLIVWDVDRHAALARMRQALADYRIVGVSTNIDFLSRLVACPAFANADLDTGLIERSR
                     DFLFPAHSEPPKSVFFVAAVAGLLREHAAALARASQSGDPWSPWSLHDGWRLNLQSRR
                     TLTYRHGDTLHEVLVAYEADGWRLTLGGESVLARGRILDSGQFAGQLAVELDDRRLIA
                     SVVAVTEKNEQKRHVFLNGSNYVILRDDPLHLVAAGGAQGGGLTAPMPGKVIALLATV
                     GQKVDKGAPLLILEAMKMEHTITAPKNGRVRAFRYAAGDQVADGAELVDFVAGEGEP"
     gene            complement(3428..4222)
                     /locus_tag="PLL14_02640"
     CDS             complement(3428..4222)
                     /locus_tag="PLL14_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG02847.1"
                     /translation="MSEYQSILTEVDGYVGILTLNKPQRHNAFDEQLIAEMIAGLREL
                     AADPRVRAVVLSSTGKSFCAGADLQWMKRAAGYSDQENLQDARRLAELMATLNELAKP
                     TIARVQGPAHGGGVGLIAACDIAVGTYDALFALSEVKLGIVPAVISPYVLAAIGERYC
                     RRYMLTAERFSAAEAYRIGLLHELVPGEEQLDEAIAEIIDSLLANGPQAQAECKSLIR
                     IVAGQPIDEETIDETARRITRIRASPEGREGLAAFLEKRPPNWVAD"
     gene            complement(4232..5848)
                     /locus_tag="PLL14_02645"
     CDS             complement(4232..5848)
                     /locus_tag="PLL14_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG02848.1"
                     /translation="MPVLQTRLDARSADFQANAAAMRQIVADLRATVDRIALGGPEAA
                     RQKHLARGKLLPRERVNALIDPGSAFLELSQLAAYGMYGAQPFEVPAASLITGIGRVN
                     GVECMIVANDATVKGGTYYPLTVKKHLRAQEIAGENRLPCIYLVDSGGAFLPLQDEVF
                     PDRDHFGRIFYNQANLSAAGIPQIAAVMGSCTAGGAYVPAMSDESIIVKEQGTIFLGG
                     PPLVKAATGEVVSAEDLGGADVHTRISGVADHYAENDAHALAIARRIVGNFNWRKRPP
                     LRLSEPCAPLHAPEELYGVIPTDARKPFDVREIIARLVDGSDFDEFKARYGTTIICGF
                     ARLCGYPLGIVANNGILFSESSLKAAHFIELCAQRGIPLLFLQNITGFMVGRKYENGG
                     IARDGAKMVTAVACARVPKFTVVIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGG
                     EQAAKVLATVRRDGLEAAGKAWSADQEEAFKAPIRAQYELQGHPYYASARLWDDGVID
                     PADTRRVVGLGLSAALNADAEATKFGIFRM"
     gene            complement(5848..>6246)
                     /locus_tag="PLL14_02650"
     CDS             complement(5848..>6246)
                     /locus_tag="PLL14_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOG02849.1"
                     /translation="RSARFLDVPYTQPANFPLATMQAARAYYWLHDQDCATARAFAHS
                     LYRGYFIDGRDISDPEVVLELAARHGADRATLAAALNDAPLKERLKAECEAAIAKGVF
                     GSPYFIVDDEPFFGVDRLPQLERWLASGGF"
BASE COUNT         1068 a         2134 c         2031 g         1013 t
ORIGIN      
        1 cgaccgcagc cgaggcaata acccgaatcc gggtcgggca tgcagatgcc gacgcaaaga
       61 tattcgtctt cggaatcggt catggtcaac gctcgcaatc gccacggaag tcaccgcccg
      121 cggcacaccc gagcggatcg tgctcgacgc ggcggcgctc gaccgccagc agaacccgca
      181 gcctttgcgc atcggaggca cggctccaga ccgcaatctc ttcgatcgtc cggaagcagc
      241 cctgacacaa accggtctgc tcatccatgc ggcagacatt gatgcacggc gagacgacca
      301 tcgaggtcaa gagcgttcct ccgaatgcac gacgcgcgcg tgcgtgctgc cctcagcagg
      361 catcagcatc cggcaccgca ccgggcgagc agggcacgcg ccgctttcga cgccggttcg
      421 cggccgaggt ggccggcgat gaactggcct gcggccagca attgttccat gtcgactccg
      481 gtttcgatgt ccagcccctg caggagatag accacgtctt cggtggccac gttgccgctc
      541 gccccggcgg cgtatggaca gcccccgaga ccggcgatcg aactgtcgaa gaccgccatg
      601 ccgagttcga gcgaggcgta gatgttggca atggccatcc cgtaggtgtc atggtaatgc
      661 ccggcgagcc ggtcgatcgg cagcaacgct gcgcaggctt cgatcatccg ctgtaccgat
      721 gccggcgtac cggcgccgat ggtatcgccg agcgagacct cgtagcaacc catttcgaac
      781 aactgccgcg ctacccgcgc cgtcttcgcc ggatcaaccg cgccttcgta cggacaggcc
      841 acgccacacg agacatagcc gcgcaccggg atctccagcg cgctggcggc gtcgagcacg
      901 gcggcaaagc gcttcaggct ctcggcgatg ctgcagttga tgttcttctg cgagaaggtc
      961 tccgaggccg cggcgaagat cgccacttcc agcgcgccgg cttcgagcgc cgcgtcaaag
     1021 cccttgaggt tcggcgtcaa gaccggatag gaaacctgcg accgacgccg cgggctggcc
     1081 agcaccgcgc gcagcacctc ggcattgtcg gccatctgcg gcacccactt cggcgacacg
     1141 aaggacgtgg cttcgatcgc cgacaagccg gcggcagcca gccgttcgat cagttcgatc
     1201 ttgatcgccg caggaacgag ctgccgctcg ttctgcaggc cgtcacgcgg cccgacctcg
     1261 accagtttca cccgttgcgg caggctcatg gctcgccctc gccagcgacg aaatcgacca
     1321 gttccgcacc gtccgcgacc tgatcgccgg ctgcgtagcg gaaagcccgc acccggccgt
     1381 tcttcggcgc ggtgatcgtg tgctccatct tcatcgcttc gagaatcagc agcggcgcgc
     1441 ccttgtccac cttctgcccg accgtcgcca gcagggcgat gaccttgccg ggcatcggcg
     1501 cggtcagacc accgccctgc gcgccgccgg cagcgaccag gtgcagcgga tcgtcgcgaa
     1561 ggatgacgta gttgctgccg ttgaggaaca cgtgccgctt ctgttcgttt ttctcggtca
     1621 ccgcgacgac gctggcgatc aggcggcgat cgtcgagttc gacggccagt tgtccggcga
     1681 actggccgct gtcgaggatc cggccgcgcg ccagcaccga ttcgccgccc agcgtcaaac
     1741 gccagccgtc ggcctcatag gcgaccagga cttcgtgcag ggtatcgccg tggcgatagg
     1801 tcagcgtgcg tcgcgactgg agattcagcc gccaaccgtc gtgcaggctc cacggcgacc
     1861 agggatctcc cgactggctg gcgcgcgcca gcgcggcggc gtgctcgcgc agcaggccgg
     1921 cgaccgcggc gacaaagaag acgctcttcg gtggttcgct gtgcgccggg aagagaaagt
     1981 cgcggctgcg ctcgatcagg ccggtgtcga gatcggcgtt ggcaaaggcc gggcaggcga
     2041 ccaggcgcga aagaaagtcg atgttggtgc tgaccccgac gatgcggtag tcggccagcg
     2101 cctgccgcat gcgcgccagc gcggcgtggc ggtcgacgtc ccagacgatc agcttggcga
     2161 tcatcgggtc gtaataaggc gtgatttcgt cgccctgctc gatgccggta tcgacgcgga
     2221 cgttgagact ctcgggcggg cgcgcgagat gcagcaggcg acccgtcgac ggcaggaaat
     2281 cgtgctcggg attctcggca tagatgcggg cttcgagcgc atgtccacga atgtcgagtt
     2341 gctcctggcg cagcggcagc ggctcgccgc cggcgacgcg caactgccat tcgaccagat
     2401 cctggccggt gatcatctcg gtcaccggat gctcgacctg caggcgggta ttcatctcca
     2461 tgaaatagaa gcggccgtcc tgcgcgaagc tgtcgccggc gatgaattcg acggtgccgg
     2521 cgccgacgta gccgaccgcg cgcgccgcct cgacggccgc ctggccgatc agcgcccgcc
     2581 gcgccggcgt catgcccggc gccggcgcct cttcgagcac tttctggtgg cggcgctgca
     2641 ccgagcagtc gcgctcgaag agatgcacgc agttgccctg cgtgtcggca aagatctgga
     2701 tctcgatgtg ccgcgagcgt cgcagatact tctcgatcag cacctgatcg gatccgaaag
     2761 cggagatggc ctcacgctgg catgacgcca gtgcggccag aaaatcttca ccgcgttcga
     2821 ccaggcgcat gcccttgccg ccacccccgg cggctgcctt gatcaacacc ggataaccga
     2881 tggcctcggc ctgccgctgc agaaaggcgg gttcctgatc gtcgccgtgg taacccggcg
     2941 tcagcggcac accggccgcg gccatcagcg tcttggccgc cgacttcgag cccatcgccc
     3001 ggatcgccgc cgccggtgga ccgatgaaaa caatgcccgc ggcgtcgcag gcgtcggcga
     3061 aacggtcgtt ttcggagaga aagccgtagc ccggatgcac cgcctgcgcg ccggtgcgcg
     3121 cggcggcatc gaggatgcgt tcgccacaca gatacgatgc gcgcgccgcc gcaggaccga
     3181 tgcacaccgc ttcgtcggcg acgcgcacgt gccgggcttc ggcatcggct tccgaataga
     3241 cggcgacggt gcgcacgccc atccggcgcg cggtccggat cacgcggcag gcgatctcgc
     3301 cgcggttggc aatcagaatc ttggtaaaca tcggcaatct ttcatcgagc ccactggtgc
     3361 ccgcacaagg cctcggcggc gtccggcagg cgctgcgggg agcaagctcg gccgaccagc
     3421 cgccacgtca atcggcgacc cagttgggcg gccgcttttc aagaaaagcc gccagcccct
     3481 cgcggccttc gggactggcg cgtatgcgcg tgatccgccg cgccgtttcg tcgatggtct
     3541 cctcgtcgat cggctgcccg gcgacgatgc ggatcagcga cttgcactcg gcctgcgcct
     3601 gcggtccgtt ggccagcagg ctgtcgatga tctcggcaat cgcctcgtcg agttgctcct
     3661 cgccgggaac gagttcatgc agcaaaccga tgcggtaggc ctcggccgcc gagaaacgct
     3721 cggcagtcag catgtagcgg cggcaatagc gctcgccgat cgctgccagc acatacggac
     3781 tgatcaccgc cggcacaatg cccagcttca cctcgctgag cgcgaacagc gcatcgtagg
     3841 tgccgaccgc gatgtcgcag gcggcgatca gtccgacgcc gccgccgtgg gccgggccct
     3901 gcacgcgcgc aatcgtcggc ttcgcgagtt cgttcagggt cgccatcaac tcggcgagcc
     3961 gcctggcatc ctgcaggttt tcctggtccg aatagccggc cgcgcgcttc atccactgca
     4021 ggtcggcacc tgcgcagaag ctcttgccgg tagacgacag gaccaccgcg cgcacccgcg
     4081 ggtcggccgc cagttcgcgc aggccggcga tcatctcggc gatcagttgc tcgtcaaaag
     4141 cgttgtgccg ctgcggcttg ttcagcgtca ggatgccgac gtagccgtcg acttcggtca
     4201 gtatcgattg gtactcgctc atcaaggccc cttacatccg gaaaatgccg aacttcgtcg
     4261 cttcggcgtc ggcgttcagc gccgccgaca ggccgaggcc gaccacccgg cgggtgtccg
     4321 cggggtcgat cacgccgtcg tcccagaggc gggcgctggc gtaatagggg tggccctgca
     4381 actcgtactg cgcacggatc ggcgccttga aggcctcctc ttggtctgcg ctccaggctt
     4441 ttccggcggc ttcgaggccg tcgcgccgga ctgtcgccag caccttggcg gcctgctcgc
     4501 cgcccatcac cgagatgcgc gcgttcggcc acatccacag gaaacgtggc gaataggcgc
     4561 gcccgcacat gccgtagttg ccggcgccga aggaaccccc gatgaccacc gtgaacttcg
     4621 gcacgcgcgc gcaggccacg gcggtgacca tcttggcccc gtcacgcgca atgccgccgt
     4681 tttcgtactt gcgaccgacc atgaaaccgg tgatgttctg caggaacaac aacgggatgc
     4741 cgcgctgcgc gcacagctcg atgaaatgcg cggccttcag cgacgactcc gagaacagga
     4801 tgccgttatt ggcaacgata cccagcggat atccgcagag gcgtgcgaaa ccgcagatga
     4861 tggtcgtccc gtagcgcgcc ttgaactcgt cgaaatccga accatcgacc aggcgggcga
     4921 tgatctcgcg cacgtcgaag ggcttcctgg cgtcggtcgg gatcacgcca tagagttcct
     4981 cgggcgcatg gagcggcgcg cacggctcgc tcaggcgcag cggcggacgc ttgcgccagt
     5041 tgaagttgcc gacgatgcgc cgggcgatcg ccagcgcatg cgcgtcgttc tcggcgtaat
     5101 gatcggcgac accggaaatg cgggtatgca cgtcggcgcc gccgagatcc tcggcgctca
     5161 ccacctcacc ggtggccgcc ttgaccagcg gcggcccgcc gagaaagatc gttccctgct
     5221 ccttgacgat gatcgattcg tccgacatcg ccggcacgta ggcaccaccg gcggtacacg
     5281 agcccatcac cgcggcgatc tgcggaatgc ccgccgccga cagattggcc tggttgtaga
     5341 agatgcggcc gaaatggtcg cggtcgggaa agacctcgtc ctgcagcggc aggaaagcgc
     5401 cacccgaatc gaccaggtag atacacggca agcggttctc gccggcgatc tcctgcgccc
     5461 gcaggtgttt cttcaccgtc agcgggtaat aggtgccgcc cttcaccgtg gcatcgttgg
     5521 cgacgatcat gcactcgaca ccattgaccc gcccgatgcc ggtgatcagc gaagctgcag
     5581 gaacttcgaa aggctgtgcg ccgtacatgc cgtaagccgc cagttgcgac agttcgagaa
     5641 aggccgaacc cggatcgatc agcgcattga cccgctcgcg cggcagcagc ttgccacgtg
     5701 ccaggtgttt ctggcgcgcc gcttccggac cgccgagcgc aatccggtcc accgtcgcgc
     5761 gcaggtcggc gacgatctgg cgcatcgccg ccgcgttggc ctgaaagtcg gcgctgcgcg
     5821 cgtcaagccg ggtttgcaga acaggcatca gaaacctccg ctggccagcc agcgttcaag
     5881 ttgtggcaga cggtcgacgc cgaagaatgg ctcgtcgtcg acgatgaagt acggcgagcc
     5941 aaagaccccc ttggcgatcg ccgcctcgca ttcggctttc aggcgttcct tcagtggcgc
     6001 gtcgttgagc gctgccgcca gtgtagcgcg gtcggcgcca tgccgtgccg cgagttcgag
     6061 caccacctcc gggtcggaaa tgtcccgccc atcgatgaag tagccgcgat agagcgaatg
     6121 cgcgaatgcg cgcgccgtcg cgcagtcctg gtcatgcagc cagtagtacg cgcgcgccgc
     6181 ctgcatcgtg gccagcggga agttcgccgg ctgcgtatac ggcacgtcga ggaaacgcgc
     6241 cgaacg
//
LOCUS       DAOSIT010000063         6137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11579_length_6137_cov_9.614436, whole genome shotgun sequence.
ACCESSION   DAOSIT010000063 DAOSIT010000000
VERSION     DAOSIT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6137_cov_9.614436"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLL14_02655"
     CDS             <1..213
                     /locus_tag="PLL14_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOG02850.1"
                     /translation="FVFGSAWGHDVVSDFRDGTDRLNFHDIGVAGLQDLSLAAVGGNA
                     LIAWHGNEVLLIGVRVADLGPADFIF"
     gene            393..1571
                     /locus_tag="PLL14_02660"
     CDS             393..1571
                     /locus_tag="PLL14_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG02851.1"
                     /translation="MPIALLHQYPIAKPLSSHCAAMVIALAGLVGCGREPPKPGPAPL
                     EVTTMTVEARDVPVSAEYVAQTQSSQAVNIQARVSGFLDKRVYTEGAVVKAGQLLFQI
                     DPKPFQVQVDGAAAALQRNQASLQVATANLKRTRPLVEQNALSQKDLDDAQGQYEQAA
                     AAVAQSKAQLESARLDLSYTRITSPVAGVTSFSALAEGTYVNSQNSQLTTVSVLTPMW
                     VNFSISENEVDRLRANVRKGLLKLPADNQLLVEIELGDGTPFPHTGKITFADPSYNSQ
                     TGTFLIRATVDNPDGVLRPNQYVRARLQGAQRPNAILIPQRAVQQGAQGHFVWLVNKE
                     GQAEQRPITVGDWYRESWFVAEGLQSGEQLVVNGGQRLSVGAKVTVMPPTAKPAAAGS
                     "
     gene            1584..>4384
                     /locus_tag="PLL14_02665"
     CDS             1584..>4384
                     /locus_tag="PLL14_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG02852.1"
                     /translation="MFSRFFIERPIFAAVVAILVVVAGLVAIFVMPVQQYPTITPVQV
                     TVQATYPGADSKTLADAVAAPIEAQINGVDKMLYMSSSSSATGQLLLTVYFALDTDPD
                     IAQVQVQNRVNLALPQLPSAVTQQGVSVQKKSSTIMMLLGVTGKGGRYSPGYVANYAN
                     VYVLDALKRVPGAGQAQIMGVPDQAMRIWMNPDRMASLAITTSDIANAINTQNALFGA
                     GQIGQQPTAGAVELSFPVVTQGQFVDPRQYERIILRASQDGSAIVRVGDVARAEVGLR
                     QYIVDTQLDREPTTFIAIYQQPGANGLVVSQAVRAALAEMKSRFPEGLDVVVALDTND
                     FVRLSIEEVLKTLVEAMVLVVLIVYLFLQSLRTTLICAVAIVVALVGTFAGMLALGFS
                     VNLLTLFGLILAIGMVVDDAIVVVENVERNMTQHHLAAKEATIRSMGEIGSSLVAVVL
                     VMASVFVPAAFLPGTTGQLYRQFAITIVISVSLSGFVALTLTPAMCSLLLRHTPVAQR
                     GFFAWFNRQVEWVTLAFGHAVEWVIRRVAVALLLLAVFLYSIWHLFHLLPTSFVPNED
                     QGYAMVAIIMPQSASLDRTQAVAERVDAILAKIPGVERRSMITGYSLIDAGYKANAAT
                     FFVTFKSFDERYASIDSARSQNARAILLALYDQARHIEEAIVLPIAPPPIPGIGTTGG
                     FEFWIQDSAAGDPSKLDELTRAFLDKARARPELTALNSTFTAQAQQLRADVDRDKATL
                     LGLQVQDVYSAIQAQFGSLTASQFSQFSRVWWVIVQSDAKFRQQPEDLTRLYTRNSQG
                     KMVPLSAVVSTHWRAGPDILPHFNGFPAAKVIGNPAPGFSSGQAIAAMEAVAREVLPA
                     GYTFAWSGLAFEEKKSGGTSALAFAFGLLIVFLVLAAQYESWTLPGAVMTAVPFGILG
                     ALLTNWARGLENDVYFQIGL"
     assembly_gap    4385..4484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4485..4921
                     /locus_tag="PLL14_02670"
     CDS             <4485..4921
                     /locus_tag="PLL14_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG02853.1"
                     /translation="LTNWARGLENDVYFQIGLLVLIGLGAKNAVLRVSAAVDFRQQGK
                     SIMEATVLAGEQRLRPIIMTSLAFAFGCLPLALALGAGANARHSIGTGIIGGMIGETT
                     LAMLYVPLFFYLFDRLAERRREAMPREAADPASASSPDGRQA"
     gene            4918..>6137
                     /locus_tag="PLL14_02675"
     CDS             4918..>6137
                     /locus_tag="PLL14_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG02854.1"
                     /translation="MKRLLVCAGTALLAGCLVGPDYRRPPVDPPAMFQYAAKDAADTA
                     DTRWWKQFRDPVLDQLIIEGLAHNSNIAIAAANVEQAAALLTQSRSPLFPQLGYAATG
                     ERQRSLEPAFASLLRNYPNPSTAYEAALSASWEIDLWGRIRRQSEAALANLLATDEAR
                     RGVILSLVASVANSYLQLRGLDAQLQVARKTLSTYGESVRLFELQFKYGQVSKMNVVQ
                     AQSQYESAAAQIPLLESQIAQNQNALAVLIGRDPGPIARGKSVDELALPPVPAGLPSA
                     LLEQRPDLLQAEQALVAANAQIGAARALYFPAISLTGAFGGASSELSNLFKGSARVWN
                     YSGSLVGPIFTFGAVSGQVAQAEAGQKAALLNYQLAIRNAFADVDNALVANQKLQEQL
                     AAQQRLVVALTEYAR"
BASE COUNT          978 a         2043 c         1962 g         1054 t
ORIGIN      
        1 ttcgttttcg gttccgcctg gggccacgat gtcgtgagcg atttccggga tggcacggat
       61 cgtctgaact ttcatgacat cggtgttgcc ggcctccagg atctgagtct cgccgcggtc
      121 ggcggcaacg cgctgatcgc ctggcatggc aacgaggtcc tgctgatcgg agtccgggtc
      181 gccgatctgg gcccggcgga cttcatcttc tgaccgggtc agcccgccgc cgcggtgcat
      241 gcaccacgac ggcaccctgg cggccggcgc caatccacgc ttccctttgg cgtccacgcg
      301 cttgactgtg ctgtgactgc cggagatact ttcgggcaga ccgttgagtg gtccattcgc
      361 ctgcctgcgc tcgccaattg tcggaaaccc cgatgcccat cgcactgctg caccagtacc
      421 ccattgccaa accactgagc agccattgcg ccgcgatggt cattgcgctg gctggcctgg
      481 tcggctgcgg cagggagccg ccaaagccgg gtccggcacc gctcgaagtc acgaccatga
      541 cggtcgaagc gcgcgacgtc ccggtgtcgg ccgaatacgt ggcgcagacg cagagttcgc
      601 aggcggtcaa tatccaggcg cgggtttcgg gctttctcga caagcgcgtc tataccgaag
      661 gtgcggtggt caaggccggt cagttgctgt tccagatcga ccccaagccg ttccaggtac
      721 aggtcgatgg cgccgccgcc gcgctgcagc gcaaccaggc ttcgctgcag gtggcgacgg
      781 ccaatctcaa gcgcaccagg ccgctggtcg agcagaacgc gctgtcgcaa aaggacctcg
      841 acgacgcgca gggtcagtac gagcaggccg ccgcggccgt ggcgcagtcg aaggcgcagc
      901 tcgagtcggc caggctcgac ctgtcgtata cccgaatcac ctcgccggtt gccggcgtga
      961 cgagtttctc agcgctcgcc gaaggcacct atgtcaactc gcagaacagc cagttgacga
     1021 ccgtttcggt gctcaccccg atgtgggtga atttcagcat ctcggagaac gaagtcgacc
     1081 gcctgcgtgc caacgtcagg aagggactgc tcaagctgcc cgccgacaac cagctgctgg
     1141 tcgaaatcga gctgggtgac ggcacgccat tcccgcatac cggcaagatc acttttgccg
     1201 atccctcgta caactcgcag accgggacct tcctgatccg cgccacggtc gataacccgg
     1261 acggcgtgct gcgtcccaac cagtacgtgc gcgcgcgcct gcagggagcg caacggccga
     1321 acgccatcct cattccgcag cgcgccgtgc agcagggcgc gcagggccat ttcgtctggc
     1381 tggtcaacaa ggaaggccag gccgagcagc ggccgattac cgtcggcgac tggtaccgcg
     1441 agagctggtt cgtcgccgag gggctgcagt ccggcgagca gctcgtcgtc aatggtggcc
     1501 agcgcctgtc ggttggcgca aaggtgacgg tgatgccgcc gacagccaaa ccggcagccg
     1561 ccggctcctg agccgccgcc ggcatgttct cccgattctt catcgagcgt ccgatcttcg
     1621 cggcagtcgt cgcgatcctg gtggtggtcg ccggcctggt ggcgatcttc gtcatgccgg
     1681 tacagcaata tccgacgatc acccccgtgc aggtcacggt gcaggcaacc tatccgggcg
     1741 ccgattccaa gaccctggcc gacgcggtcg cggcgccgat agaggcgcag atcaacggtg
     1801 tcgacaagat gctctacatg agttcgagca gttcggccac cggccagctc ctgctgaccg
     1861 tctacttcgc cctcgacacc gaccccgaca tcgcgcaggt gcaggtgcag aaccgcgtga
     1921 acctggcgct gccgcaactg ccttcggcgg tgacgcagca gggcgtgtcg gtgcagaaga
     1981 aatcttcgac gatcatgatg ctgctcggcg tcaccggcaa gggcggccga tacagcccgg
     2041 ggtatgtcgc caactatgcc aacgtctacg tgctcgatgc gctgaagcgc gttcccggcg
     2101 ccggccaggc ccagatcatg ggcgttcccg accaggccat gcgcatctgg atgaacccgg
     2161 accgcatggc ctcgctggcg atcaccacca gcgacatcgc caatgcgatc aacacccaga
     2221 atgcgctgtt cggcgccggc cagatcggac agcagccgac cgctggcgcc gtcgaactca
     2281 gctttccggt ggtcacgcag ggccagttcg tcgacccgcg ccaatacgaa aggatcatcc
     2341 tgcgcgccag ccaggacggc agcgcgatcg tccgcgtcgg agacgtggcg cgcgccgaag
     2401 tgggcctgcg acagtacatc gtcgataccc agctcgaccg cgagccgacg accttcatcg
     2461 ccatctacca gcagccgggc gccaacggcc tggtggtttc gcaggccgtg cgcgcggcgc
     2521 tggcggagat gaagtcgcgt tttcccgaag gtctcgacgt ggtggtggcg ctcgacacca
     2581 acgacttcgt gcgcctgtcg atcgaggagg tgctgaagac gctggtcgag gcgatggtgc
     2641 tggtggtgct gatcgtctac ctgtttctgc agagcctgcg cacgacgctg atctgcgcgg
     2701 tggcgatcgt cgtcgcgctg gtcggcactt ttgccggcat gctggcactt ggtttttcgg
     2761 tcaacctgct gaccctgttc ggcctgatcc tggcgatcgg catggtcgtc gacgacgcca
     2821 tcgtcgtcgt cgagaacgtc gagcgcaaca tgacgcaaca ccatctggcg gcgaaggagg
     2881 cgacgatccg ctcgatgggc gagatcggca gttcgctcgt cgccgtggtc ctggtgatgg
     2941 cttcggtctt cgtgccggcc gcattcctgc ccggcaccac cggccagctc taccgccagt
     3001 tcgcgatcac catcgtcatc tcggtgagcc tctcgggttt tgtcgcactg acgctgaccc
     3061 cggccatgtg ttcgctgctg ctccggcaca ccccggtggc gcagcgcggc ttcttcgcct
     3121 ggttcaaccg ccaggtcgag tgggtgacac tggctttcgg gcatgcggtc gaatgggtca
     3181 tcaggcgggt agcggtggcc ctgctgctgc tggcggtgtt cctgtacagc atctggcatc
     3241 tgtttcacct gctgccgacc agcttcgtgc cgaacgagga tcagggttac gcgatggtgg
     3301 cgatcatcat gccgcagtcc gccagcctcg atcgcacgca ggccgtcgcc gaacgcgtcg
     3361 atgccatcct ggcgaagatt ccgggtgtcg aacggcgctc gatgatcacc ggctacagcc
     3421 tgatcgacgc cggctacaag gcgaatgcgg cgaccttctt cgtcaccttc aagtccttcg
     3481 acgaacgtta cgcctcgatc gactcggcgc gcagccagaa cgcccgcgcc atcctgctcg
     3541 cgctctacga ccaggccaga cacatcgagg aggcgatcgt gctgccgatc gcgccgccgc
     3601 cgatcccggg cattggtacc accggtggtt tcgaattctg gatccaggac agcgccgccg
     3661 gcgacccgtc gaagctcgac gagctgacgc gcgccttcct cgacaaggcg cgtgcgcgtc
     3721 ccgagctgac tgcgctgaac agcaccttca ctgcgcaggc gcagcagttg cgggccgatg
     3781 tcgatcgcga caaggcgacg ctgctcggcc tgcaggtaca ggatgtctac agcgcgatcc
     3841 aggcgcagtt cggctcgctg accgccagcc agttcagcca gttcagccgc gtctggtggg
     3901 tcatcgtgca gtcggatgcg aaattccgcc agcaacccga ggatctgacg cggctgtaca
     3961 cccgcaacag ccagggcaag atggtgcccc tgtcggcggt ggtcagcacg cactggcgcg
     4021 ccggcccgga catcctgccg catttcaacg gcttcccggc ggccaaggtg atcggcaacc
     4081 cggcgccggg tttcagttcc ggacaggcga tcgcggccat ggaggcggtg gcgcgcgaag
     4141 tcctgccggc gggctatacc ttcgcctggt cgggtctcgc cttcgaggag aagaaatccg
     4201 gcggcacctc ggcgctggcc tttgctttcg ggctgctcat cgtcttcctg gtgctcgccg
     4261 cgcagtacga gtcgtggacg ctgccgggcg cggtgatgac cgcggtgccc ttcggcatcc
     4321 tcggcgcgct gctcaccaac tgggcgcgcg ggctggagaa cgacgtctac ttccagatcg
     4381 gcctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgctca ccaactgggc
     4501 gcgcgggctg gagaacgacg tctacttcca gatcggcctg ctggtgctga tcggcctcgg
     4561 tgccaagaac gcggtcctgc gggtgtcggc ggcggtcgac ttccgccagc agggcaagtc
     4621 gatcatggaa gcgaccgtgc ttgccggcga acagcgattg cgaccgatca tcatgacctc
     4681 actggccttc gccttcggtt gcctgccgct ggccctggcg ctcggcgctg gcgccaacgc
     4741 ccgtcattcg atcggcaccg gaatcatcgg cgggatgatc ggcgagacga cgctggccat
     4801 gctctacgtg ccgctgttct tctacctctt cgaccgcctc gccgagcgcc gccgcgaggc
     4861 gatgccgcgg gaagccgctg acccggcgtc ggcgtcgtcg ccggacggcc gccaggcatg
     4921 aagcgtctgc tcgtctgcgc cggcacggcg ctgctcgccg gctgcctggt gggtcccgac
     4981 taccggcgcc cgccagtcga tcccccggcg atgttccagt acgcggcgaa agacgctgcc
     5041 gacaccgccg acacgcggtg gtggaagcag ttccgggacc cggtgctcga tcaactgatc
     5101 atcgagggac tggcgcacaa cagcaacatc gcgatcgccg cggccaacgt cgagcaggcg
     5161 gcggcgctgc tcacacaatc gcgctcgcca ctgtttccgc aactcggcta cgcggccacc
     5221 ggcgagcgcc agcgctcgct cgaaccggcg tttgcctcgc tactgcgcaa ctatccgaac
     5281 ccgagcacgg cttatgaggc ggcgctctcg gcaagctggg agatcgacct ctggggccgc
     5341 attcggcgcc agtccgaggc ggcgctggcc aacctgctgg ccactgacga ggcgcggcgc
     5401 ggcgtgatcc tgtcgctggt cgcttcggtg gccaacagct acctgcaatt gcgcggcctc
     5461 gacgcgcaac tgcaggtcgc ccgcaagacc ctgtcgacct acggcgaatc ggtcaggctt
     5521 ttcgaactgc agttcaagta cggccaggtg tcgaagatga acgtcgtgca ggcacagtcg
     5581 caatacgagt cggcggcggc gcagattccc ctgctcgaat cgcagatcgc acagaaccag
     5641 aacgcgctgg cggtcctgat cggccgcgat ccgggtccga tcgcccgcgg caagtcggtc
     5701 gatgaacttg ccctgccgcc ggtgccggcc ggcctgccgt cggccttgct cgaacagcgc
     5761 cccgacctgc tgcaggccga gcaggcgctg gtggcggcca atgcgcagat cggcgcggcc
     5821 agggcgctgt actttcccgc gatctcgctg accggcgctt ttggtggtgc cagttccgag
     5881 ctgtcgaacc tgttcaaggg ctccgcgcgc gtctggaact acagcggttc cctggtcggc
     5941 ccgattttca ccttcggcgc ggtcagcggc caggtcgcgc aggccgaagc cgggcagaag
     6001 gcggcgctgc tcaactacca gttggcgatc cgcaacgcct ttgccgatgt ggacaacgcg
     6061 ctggtcgcca accagaagct gcaggagcaa ctcgccgcgc aacagcggct ggtcgtcgcc
     6121 ctgaccgagt acgcgcg
//
LOCUS       DAOSIT010000064         6107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11654_length_6107_cov_7.639128, whole genome shotgun sequence.
ACCESSION   DAOSIT010000064 DAOSIT010000000
VERSION     DAOSIT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11654_length_6107_cov_7.639128"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLL14_02680"
     CDS             <1..591
                     /locus_tag="PLL14_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOG02855.1"
                     /translation="LKALVVSLALVGAAAPCSASVITFEGFPDLTVFTTQDFGSGVTF
                     AGATILTLGGTLNPAFPPHSGSNVVYNPSGAMTLNFTTAVDFFSGFFTYNEPLVIQAY
                     DIDNNILDTANGACTANYIGSGTGCLDNEFLQVDAIGAIAKVVIAGGGGNNFTLDDAE
                     FTGSRDPGLPEPATMAMLLGGLGALGLSRRRRAAAL"
     gene            complement(724..1005)
                     /locus_tag="PLL14_02685"
     CDS             complement(724..1005)
                     /locus_tag="PLL14_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02856.1"
                     /translation="MNCCGNNRAALAQPSQAKPLGAGDRTSRQAAKRPAAEEVHFQYT
                     GKTALSVRGMFSRRIYRFAAPMAVLEVEVRDAPGLAAVPLLKRVSVPDC"
     gene            complement(986..1372)
                     /locus_tag="PLL14_02690"
     CDS             complement(986..1372)
                     /locus_tag="PLL14_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02857.1"
                     /translation="MQQTDGVPWFLFGVATLAVWRLTHLLHAEDGPFELCVRLRHLLG
                     EGFFGKLLDCFYCLSMWTAAPAAWLVGGDWREWMLLWLALSATAILVERVHAGLLARE
                     QACSLPAIYSEDAAIPEEETDELLRK"
     gene            complement(1388..3631)
                     /locus_tag="PLL14_02695"
     CDS             complement(1388..3631)
                     /locus_tag="PLL14_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02858.1"
                     /translation="MPLIMHGNWTVAVKEKHAAFPQRFIISGAASGNGTYVAPHVPVP
                     VTGGTWSVRIQSDPGGSSWADSEYQITFPSKSAGQYRFDLQSNDVWGGDKDFNDLVLT
                     FSTPVTETDFLVYGHVSTYSGCSYNPCYPGYIYIESALALANARRFPILRKAIEQLYP
                     QSVPAQRIPLPDPPPDLPAALLGGPAYKPVLIPVQGKTYTPVKKAQVMRIMPVERAAG
                     TDNETATTAEAHRVPVRTVEVAQAVSEIAGLDKVALGRLLDIGIRNCQTESLVNAALR
                     FQEYDRTLAELGGGPYTGEGNREELGQASTDRNGNYIFRFSRTLAQLIDETNSDVALG
                     ENEVLEAMPDLIVQVLGATMPGGTPYETAPCWNMPLVKRLNICIPSSYWHTPSGCHGK
                     PISHIGFIPVGKTATVTLDADGRVTCTDTTKIDIPQTHCAAWWGALRMSACIGKYDRV
                     PHYTIEYRARRPDNSWTNWSIYQEALMLDNWKTTVNEWVATKAGPFIHNLELVKGQPK
                     QDVLAYNNIQGNMDWSGPDWFIKAIIPSWAYSYQGGPGSVQFRLKAYGPDGKQIQIWS
                     DPVTSAPLYQDSIVLYVDHTGPELNFKDVTIGTVTANPCPLFTLTGSELVNATLDLKF
                     KAVQRQGFLGAYTLSVSKCNTPNFPLEDLASAHPLHLDYLAGPSPCGDLFGTLFGVDV
                     DADVDDYVAVQLNPPGNASWLEATETLSSFTLNLSASVRRTDGHSSSYPGYYGPLQYN
                     LVIQRGS"
     gene            4082..>6107
                     /locus_tag="PLL14_02700"
     CDS             4082..>6107
                     /locus_tag="PLL14_02700"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HOG02859.1"
                     /translation="MTRQIVVCMDGTWNDPIEQTNVYRLFQMLAGEEQQVEENGPIRS
                     HLVKSSEELVAFYLQSIGNGGRSQGLLGGTQGIGLHDSMIDAYLLISQVYQRGDKIWL
                     FGFSRGAWAARSLGAFIARSGLIPAADADDDSAADQAERIWLNYKEDRGKKRGGRFWR
                     HHDETPIRMIGVWDTVGELGVPVFNGLRLVDRDELRFLKFSDCELSPRVEYARQALAI
                     DEERTDFVPTLWDEREGIEQVWFPGAHADVGGGYENHGLSDIALEWMMQEVNKLDADL
                     WLSPSLLSQALTPDCLQDRHDETRGPVWRTRPSKERKIPEAAELHPSVLQRLQERADY
                     RPRALEDLPACAGFYPKDEPHPEEELQQEREALPFRKLPVDGSTSFPAYAHKWWNASG
                     VEVDVGERYRIEASGAWIDKKVPAGAEGYESKAWLLHLAEGSRRMEERPWFSLIAAIH
                     PRPDLEANNVDSENMLSGFIESAINGVARIDDESSLVATPNGCEIEIIEPGFLYFFAN
                     DSSFSYGNNSGFLTVQVTRLPWSTEDDHKPALLPQLVPGSLDVKLYFTPGTCSLAIHI
                     ALREAGLDFDLVRVDIRQRKLADGSDFTAINPKGYVPVLELDDGVCLTEVPAIVQYIA
                     DRMPESGLAPRPYTLERYQLQEWLGFINSELHQAFVPLLKPDTPEEYKRRV"
BASE COUNT         1223 a         1867 c         1851 g         1166 t
ORIGIN      
        1 ttgaaagcac ttgtcgtatc gctcgcactc gtcggcgcgg cggctccatg ttcggcatcc
       61 gtcatcacct tcgaaggttt tcctgacctg accgtgttca ccacccagga cttcggctcg
      121 ggcgtcacct tcgccggcgc caccatcctg accctgggcg gcaccctgaa tcccgcgttt
      181 ccgccgcatt ccgggagcaa tgtcgtctat aacccgagcg gggcgatgac gctcaatttc
      241 accacggccg tcgatttctt cagcggcttc ttcacctaca atgagccgct ggtgattcag
      301 gcctacgaca tcgacaacaa tattctggat acggccaacg gcgcgtgcac cgccaattac
      361 atcggatccg gtacgggttg ccttgacaat gagtttctgc aggtcgacgc cattggcgcg
      421 attgcgaagg ttgtgatcgc cggcggtggc ggcaacaact tcacgctcga cgatgccgag
      481 ttcaccggtt cgcgcgatcc tggcctgccg gaaccggcga cgatggccat gctgcttggc
      541 ggcctgggcg ctttggggct gagccgccgt cgccgcgctg ctgcgctcta agtagccgtt
      601 tgatcctcga aaaccggccg gagcaaaatg ccccggccgg ttttttgcgt ccatgtgtac
      661 ctgccgacga acggacggaa ccgctgaaat gaagcctgga gccttatcaa gcagtttgct
      721 cgctcagcaa tccggcacac tcaccctctt caaaaggggc accgcagcca agccaggggc
      781 gtcgcgcact tcgacttcca gcaccgccat cggggcggca aagcgataga tccgtcggga
      841 gaacatgccg cgcacgctca atgcggtctt gcccgtgtat tgaaaatgga cctcttcagc
      901 cgccggtcgc tttgcagcct gccgcgacgt tcgatcgcct gcaccgagcg gttttgcctg
      961 gcttggctgc gccaaagctg cacggttatt tccgcagcaa ttcatcagtt tcctcctccg
     1021 gtatcgctgc atcttccgaa tagatggccg gcaggctgca agcctgttcc cgggccaaca
     1081 atccggcatg gactctttcc acgaggatgg cggtcgcgga aagcgccaac cacagcagca
     1141 tccactcccg ccagtcaccg ccaacaagcc aagctgccgg ggctgccgtc cacatgctca
     1201 ggcagtagaa gcagtccaga agcttcccga aaaagccctc tcccaggagg tggcgcagac
     1261 gcacgcaaag ctcaaagggc ccatcctcgg cgtgcagcag gtgcgtcaga cgccaaaccg
     1321 ccagcgtcgc gacgccgaac agaaaccagg gaacaccatc cgtctgctgc atgccgatgt
     1381 cgcgacctta tgagccgcgc tgaatcacca ggttgtactg cagcggtccg tagtaacccg
     1441 ggtaactgct gctgtgacca tcggtgcggc gcacgctggc gctcaggttg agggtaaagc
     1501 tcgacagcgt ttcggtggcc tccagccagg acgcatttcc gggcgggttg agttgaaccg
     1561 cgacgtaatc gtccacgtcg gcatccacgt cgacgccgaa gagcgtgccg aacaggtccc
     1621 cgcagggaga tgggccggcc aggtagtcga gatgcagggg atgagcggat gccaggtctt
     1681 ccagcggaaa gttgggggta ttgcacttgc tgacgctcag ggtgtaggcg cccaggaaac
     1741 cctgacgctg gaccgccttg aacttcaggt caagtgtcgc gttcaccaac tcgctgccgg
     1801 tcagggtaaa caaggggcac ggattggcgg tgacggttcc gatggtcacg tccttgaagt
     1861 tcaactccgg gccggtatgg tcgacataga gcacgatgct gtcctgatac agcggtgccg
     1921 aggtcaccgg atcgctccag atctggatct gcttgccatc cggaccgtag gccttcagac
     1981 ggaactggac gctgcccggc ccaccctggt acgaataggc ccaactggga atgatcgcct
     2041 tgatgaacca gtcggggccg gaccagtcca tgttgccctg gatgttgtta taggccagga
     2101 catcctgttt gggttgccct ttgaccagtt ccaaattgtg gatgaaagga ccggccttgg
     2161 tggccaccca ctcgttcacc gtggtcttcc agttatccag catcagagct tcctgataga
     2221 tactccagtt ggtccaactg ttgtctggcc gcctggcacg atattcaatg gtgtaatgcg
     2281 gcacccgatc atactttccg atgcaggcac tcatgcgcag ggcaccccac caggccgcgc
     2341 agtgagtctg cgggatgtcg atcttcgtgg tgtcggtgca ggtcacgcgc ccgtcggcat
     2401 ccagcgtgac ggtagcagtc ttgccaaccg ggatgaaacc gatgtgcgag atcggcttgc
     2461 catgacaccc gctgggagtg tgccaataac ttgagggaat gcagatgttc aggcgcttga
     2521 ccaacggcat attccagcaa ggcgcggtct cgtagggagt tccccctggc atggtggcgc
     2581 cgagcacctg cacgatcaga tccggcatgg cttcgaggac ctcattctcg ccgagtgcga
     2641 catcgctgtt ggtctcgtcg atcagttggg ccagggtgcg gctgaaacgg aagatgtagt
     2701 ttccattccg gtccgtgctg gcctggccca actcttcgcg attgccctca ccagtatacg
     2761 gtccgccacc caactccgcg agggtgcggt catactcctg gaaacgcagt gccgcattga
     2821 ccagcgattc ggtttgacaa ttccggatgc cgatatccag caggcgaccc agggcaacct
     2881 tgtccagacc ggcaatttcg ctcacggcct gagccacctc gaccgtacgc acaggaacac
     2941 ggtgggcttc agcagtagtc gcagtttcgt tgtcggtacc ggctgcgcgt tcgaccggca
     3001 tgatcctcat cacttgcgcc ttcttcaccg gggtataagt cttgccttgc accgggatga
     3061 gcaccggctt gtaagccgga ccgcccaaca gggcggcggg cagatcgggt ggcgggtcgg
     3121 gcagcggaat gcgctgggcc gggacgcttt gcggatacaa ctgctcgatc gccttgcgca
     3181 ggatcgggaa gcggcgagca ttcgcgaggg ccagggcgct ctcgatgtaa atgtaaccgg
     3241 ggtaacaggg gttgtaggag cacccgctgt acgtgctcac gtgtccatag accaggaagt
     3301 cagtctccgt gaccggtgtc gagaaggtca ggaccaggtc attgaagtcc ttgtctccac
     3361 cccacacatc gttggactga agatcaaacc ggtactgtcc ggcagacttg ctggggaagg
     3421 tgatctggta ttccgagtca gcccaggagg aaccgccggg gtcgctctgg atccgcacgg
     3481 accaggtgcc gccggtaacc ggaaccggca cgtgcggcgc cacataggtg ccattgccgc
     3541 tggccgctcc actgatgatg aaacgctgcg ggaaagcggc gtgtttttcc ttgaccgcga
     3601 ccgtccagtt accgtgcatg atgagaggca tttgtgacgc tcctttctga gcccgcaaag
     3661 tgcaggccag gtgttgaggg aaaaatcagc cacgcgactc gcgcagggcc cgctgcgcat
     3721 cctcccggct tcacgttcga ggatacgagc ctggaagggc attgcctgct gccgtatggc
     3781 gtccgtcatc tccacccatg cagcgccgct gggagtctat cgccctgctt gccacatggt
     3841 atggaaaaac cattaattaa aagcattgta gacgaggatc gtgcgggatc aagccgtttc
     3901 gcgagaccgg agcgactggc cagacgtccg ggcaggatga ctcgtcggag gatttgagtt
     3961 ccttcgctga cccaaatcat atgacgagcg gagtatgctt gtagacccga agaccaatac
     4021 ccacccaggg taaacttttc gcgagtatcg ggttacgacg cgctgacaat gaggagaagc
     4081 aatgaccaga cagattgttg tttgcatgga tggcacctgg aacgacccca tcgagcagac
     4141 caatgtgtac cgattgttcc agatgctggc cggcgaggag cagcaggtcg aggaaaatgg
     4201 accgatccgt tcgcatctgg tgaagagcag cgaggaactc gtcgccttct atctccaaag
     4261 catcggcaac ggtgggcggt cgcaggggct gctcggcggc acgcagggaa tcggtttgca
     4321 cgacagcatg attgacgcgt atctgttgat ctcgcaggtg tatcagcgcg gcgacaagat
     4381 ctggcttttt ggtttctcgc gcggcgcctg ggctgcccgc agtctggggg ccttcattgc
     4441 ccgctcaggc ttgatcccag cggcagatgc cgacgacgac agtgcggccg accaggccga
     4501 gaggatctgg ctgaactaca aggaagatcg cggcaagaag cgcggcgggc gattctggag
     4561 gcatcacgac gaaaccccga ttcgcatgat cggcgtctgg gataccgttg gagaactggg
     4621 cgtgccggta ttcaacggcc tgcgcctggt cgaccgggac gaactccgct tcctgaaatt
     4681 cagtgattgc gaactgagtc cgcgcgtcga gtacgctcgc caggcgctgg ccatcgacga
     4741 ggaacgcacc gacttcgtac cgacgctgtg ggacgagcgc gaggggatcg aacaggtctg
     4801 gttccccggc gcacatgccg atgtcggggg cggctacgaa aaccacggtc tctcggacat
     4861 tgcgctcgaa tggatgatgc aggaggtcaa caagctcgat gccgacctgt ggctgtcgcc
     4921 cagcctactc agccaagcac tgacccccga ttgcctgcag gaccgacacg acgaaacccg
     4981 tggaccggtt tggcgaacac ggccgagcaa ggaacgcaag atccccgaag ctgcagaact
     5041 gcacccgagt gttctgcaac gcttgcagga gcgggccgat tatcgaccca gggcgctgga
     5101 agatcttccg gcatgcgccg gtttctaccc gaaggatgag ccgcaccccg aagaagaact
     5161 gcagcaggaa cgggaagccc tgcccttccg gaagctgccg gttgatggct cgacgagctt
     5221 cccggcgtat gcccacaaat ggtggaatgc ctccggcgtc gaggtggacg tgggcgaaag
     5281 gtaccgcatc gaggccagcg gcgcatggat cgacaagaag gtccccgccg gcgccgaggg
     5341 ctatgagtcg aaggcgtggc ttcttcatct cgcggaaggt agccgccgca tggaagagcg
     5401 cccatggttt tcattgatcg cggccatcca tccgcgaccc gacctcgaag cgaacaacgt
     5461 agactcggag aacatgctca gcggcttcat tgaaagcgcc atcaacgggg tcgcgcgcat
     5521 cgacgacgag agcagcctgg tcgccacacc gaacggctgt gagatcgaga tcatcgaacc
     5581 gggtttcctg tacttctttg ccaacgactc gtccttttcc tacggcaaca acagcggctt
     5641 ccttaccgtc caggtgaccc gactcccctg gtcgacggaa gacgaccata aaccggcgct
     5701 gctgccgcag ttggtccccg gcagcctgga cgtcaagctc tacttcactc ccggaacctg
     5761 ctcgctggcg attcatattg ccctgcgcga agccggtctc gacttcgacc tcgtccgcgt
     5821 cgatatccgc caacgcaagc tcgccgacgg cagcgacttt accgccatca accccaaggg
     5881 ctacgtgccg gtactcgaac tcgacgacgg cgtttgcctc accgaagtcc cggccatcgt
     5941 ccagtacatc gccgaccgca tgccggaaag cggcctggcg ccgcgaccct atacgctcga
     6001 acgctaccag ttgcaggaat ggctcggttt catcaactcc gaactgcatc aggccttcgt
     6061 cccactgtta aaaccggata cgcccgagga gtacaagcgg agggtca
//
LOCUS       DAOSIT010000065         6042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11823_length_6042_cov_7.606314, whole genome shotgun sequence.
ACCESSION   DAOSIT010000065 DAOSIT010000000
VERSION     DAOSIT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11823_length_6042_cov_7.606314"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLL14_02705"
     CDS             <1..186
                     /locus_tag="PLL14_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02860.1"
                     /translation="LASGLPVLHCFFVYPDEVKRGLLPAFARFELLGQSIAPGVNLIE
                     PCPLFFPTQCRAGADPL"
     gene            187..639
                     /locus_tag="PLL14_02710"
     CDS             187..639
                     /locus_tag="PLL14_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02861.1"
                     /translation="MLVHLRFGPLEILQRWASGEVRLLALEDFLCCFCVAQFHLVVEA
                     GLDLLGRPRSLRSNPQALLVSCLRRQFAAFVDILEVRDDVLLAALEQRGALRLRQPER
                     FVVQPHVDLDAAVIGSMDQKLALPWRVRVGNQLGHDDPAFTSARLPEN"
     gene            complement(611..1273)
                     /gene="radC"
                     /locus_tag="PLL14_02715"
     CDS             complement(611..1273)
                     /gene="radC"
                     /locus_tag="PLL14_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG02862.1"
                     /translation="MSQSIQQMPEADRPREKLLRKGAAALSDRELLAVLLGKGAPGMD
                     VMTLAGKLARVIDEKGLTVKAEDLTQFVGVGDAKATLILAAIEFARRRIKPEGAKIET
                     PADLLPHIRHYADRKQEHFLCASINGANEILNIRVVSIGLVDRSPVHPREVFADALTD
                     RASAVIVAHNHPNGGLEPSPSDIRITAQLKAAGEIVGIMLLDHIIFSRIGYFSFLEAG
                     RL"
     gene            complement(1278..4211)
                     /locus_tag="PLL14_02720"
     CDS             complement(1278..4211)
                     /locus_tag="PLL14_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG02863.1"
                     /translation="MSKKVPELAFQQHIANFLIRVHRYGVLEQSGITDTEHCIAEDQL
                     WAFLKATQADTLKKLTNDYGSDARYEVFKALRKNLEQTPLWMLLRGGLQVRGLEFRLF
                     YPKPRSTESTAVKKYGENRITFRHHFFFGETNQEIDFVVFLNGLPIVALELKHEKNQT
                     VHDAVTQFAGRDHTHRIFCHPFLYLAADTSDVKAATDPRREQNFRWHNTGLTNAPMTQ
                     DGSEYPVEFLYRELLAKDSLLEALSFFLVRVPHRDAVDDVPEHPAFTLFPRYHQSRLV
                     RKVAEDISARFADQADIGRKYLVNHSAGSGKTLTICWLADRLHSLFKPGSSEKLVDMV
                     FILTDRKSLDTHIKDEIENFTHLKDVIGLARKAEDLPRFMAERRPIIVSTQQKFAWVL
                     EQIEQKPELKKLRVAFLIDEAHRSQEGQMGAAIRLPFRAGTQAPDAADDEPGEPDPQE
                     QLAKIIREHDVNQMIVAFTATPAPATVSLFGEAFDTYTEEEAIAEGYIVDVATSIISY
                     KTLYNLHCAVVPKPDEEKLYPKSVVSKALQNVAFQDDGLIQYKAEVMLRLFEADVLPL
                     IGGRAKAMIVTSSRVAGLRYFEIIKEKLKERGGAYKVLYAFSDFTHPQTNAEIREHAI
                     NELKDGELIEQRFEGDDYRLMIVANKFQTGFDQPLLAGMFLDKPVMDRNAVQTVSRLN
                     RCHEGKDRVVVVDFTNNAKAILKAFIKYRKGTPFEPEEPDATLCKALHAEIIAAGVFT
                     QQDAADLGKLLATGTDAQVQFVVVALRKRFQEKLTDLDPRKEYVYQLSRYVRSFHFLS
                     CFFTFAPELKEFATFAEYVGPQLIKAGSVSDLMKLIRQTEVIKAAVEYQGVESGGGTV
                     KLKPGGGTKSAGLPQVKVSVQDMIAEIQARFTISDEEALYIKQVTEEKTADPVIQNTV
                     VAHRDDGIYLEGAYRVQVNGQIQTAYSDRGLFEELSDLKYTDTGGIFDIMAVTVIRHH
                     LACAA"
     gene            complement(4213..4944)
                     /locus_tag="PLL14_02725"
     CDS             complement(4213..4944)
                     /locus_tag="PLL14_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOG02864.1"
                     /translation="MSWAVARELSETMTRLLVHVEGETEETFVNEVLAAHLYEHGYTT
                     VSARLVGNARQRDRRGGIRSWSSIRQDIVRHLKEDAGCRSTTMVDYYALPQSGERAWP
                     GRAEAGLRPFAEKAGTVENALREEICAKMGADFDHQRFVPYVMMHEFEGLLFSDAAKL
                     GEGIGRADLSPAFQAIRDQFATPEEINDSPVTAPSKRIINLLPSYEKPLMGTLAVLEI
                     GLDAIRRECPFFRAWVEHLEQWPTV"
     gene            complement(4913..6022)
                     /locus_tag="PLL14_02730"
     CDS             complement(4913..6022)
                     /locus_tag="PLL14_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG02865.1"
                     /translation="MPELETITVKGFKSIAAVENLKLGAINVVIGSNGSGKSNFIGVF
                     AFLHAIRDGRLQDYVIKAGGADKVLHFGSKQTQKLQIRIAFRNECNQYDIDLEPTDAD
                     ELVPSSEVVYFWDKARYPEQPYNEVIARAGKEAGISAQRAIPVAHYVREHLGRWRLYH
                     FHDTSSTSPMKKTADVNDNRYLRPDGSNLAAFLYYLREKHAASYSLIQRTVQRVAPFF
                     EDFQLEPQQLNPDKIRLEWRHKGSDAYFDAPSLSDGTLRFIALTTLLLQPESYRPSVI
                     LVDEPELGLHPYAITLLASLIKQASTKTQVIISTQSPFLLDHFQPEDVLVADRVAGGT
                     QFTRLDPARLDSWLQDYSLGQLWEKNELGGRPGVE"
BASE COUNT         1127 a         1834 c         1745 g         1336 t
ORIGIN      
        1 ctggcaagtg gtttgccagt tcttcactgt ttttttgttt accctgacga agtgaagcgg
       61 ggtcttctac ctgctttcgc ccgctttgaa ctgctcggcc agtcgattgc gccgggcgtt
      121 aatctgatcg agccttgccc gcttttcttc cccacgcagt gtcgcgctgg cgctgatccg
      181 ctgtaattgc tggttcatct gcgattcggc ccgcttgaaa ttctccagcg ctgggcgtca
      241 ggcgaggttc gtcttctcgc cctcgaagat ttcctgtgct gtttctgcgt cgcccagttt
      301 cacctggtgg tggaagctgg cttagacctg ctcggtcgcc cgcgctccct ccgatcgaac
      361 ccacaagcac ttcttgtgag ttgcctccgg cggcagttcg ctgccttcgt agatatcctg
      421 gaggtgcggg atgatgttct gctggctgcc ctggaacaac gcggcgcatt gcgcctgcgg
      481 caacctgagc gtttcgtcgt ccagccccat gtcgatcttg acgcggccgt catcggctcc
      541 atggatcaga aattggctct tccctggcga gtgagggttg gcaatcagct cggtcacgac
      601 gaccccgctt tcacaagcgc ccggcttcca gaaaactgaa atagccgatg cgactgaaga
      661 tgatgtggtc cagtagcatg atgccgacaa tctcaccggc ggccttgagt tgggcggtaa
      721 tccggatgtc gctgggggat ggctccagac caccattcgg gtggttgtgg gcgacgatga
      781 ccgccgaggc ccggtcggtc agcgcatcag cgaaaacctc tcgcggatgg accgggctgc
      841 ggtcaaccag gccaatcgaa acgacacgga tgttcaatat ttcattggca ccgttgatgc
      901 tggcgcatag aaagtgctcc tgcttccggt ccgcgtagtg ccgaatgtgt ggcaacaagt
      961 ccgccggggt ctcgatcttc gcgccctccg gcttgatgcg gcgccgggcg aattcgattg
     1021 ccgcaagaat caaggtcgcc ttggcatcgc caacaccaac aaactgcgtc aggtcttcgg
     1081 ccttcaccgt caaacccttc tcgtcgatga cgcgcgccag ctttccggca agcgtcatca
     1141 catccattcc cggcgcccct ttccccagca gcacggccaa cagttcccgg tcgctcaagg
     1201 ccgccgcgcc ttttcgcagc agcttctcgc gtggccggtc ggcttcgggc atctgctgaa
     1261 tggattggct catcgcctca cgccgcgcag gccaggtggt gccggatgac ggtcaccgcc
     1321 atgatgtcga aaatgccacc cgtgtcggtg tatttgagat cggaaagctc ttcaaaaagt
     1381 ccgcgatcgc tgtaagctgt ctgaatctgg ccgttaacct gcacgcggta agcgccttcg
     1441 agatagatgc cgtcgtcccg gtgcgcaacg accgtatttt gaatgaccgg gtcggccgtt
     1501 ttttcctcgg tcacctgttt gatgtagagc gcctcttcgt ccgaaatggt gaacctggcc
     1561 tgaatctctg cgatcatgtc ctgaaccgac accttgacct gcggcaggcc ggcactcttc
     1621 gttccgcccc ctggcttgag cttgactgtc ccgccgcctg attcgacgcc ctgatattcg
     1681 accgcggcct tgatcacttc cgtctggcga atcagcttca tcaaatccga cacgctgcca
     1741 gccttgatga gctgcgggcc aacatactcg gcaaaggtcg caaactcctt cagttccggt
     1801 gcgaacgtga aaaagcagga gagaaaatgg aagcttctga catagcggga aagctgatag
     1861 acgtactctt tacgcggatc aagatcggtg agtttctcct ggaaacgttt gcgtagcgca
     1921 acgaccacga actgcacctg cgcatcggtg ccagttgcca gcagcttgcc aagatcggcg
     1981 gcatcctgct gcgtgaagac gcccgccgcg ataatctcgg catgcagcgc cttgcacaac
     2041 gtcgcatccg gttcttccgg ttcgaacggt gttcccttgc ggtatttgat gaacgccttg
     2101 agaatcgcct tcgcgttatt ggtgaagtcc acaaccacga cgcggtcttt gccctcgtgg
     2161 catcggttca gtcgtgaaac ggtctgcacc gcattgcggt ccattaccgg cttgtcgaga
     2221 aacatcccgg ccagcaaggg ctggtcgaac ccggtttgaa acttgttggc gacgatcatc
     2281 aggcggtagt cgtcgccctc aaagcgctgt tcgatcaact cgccgtcctt cagttcgttg
     2341 atcgcgtgct cgcgaatttc ggcattggtt tggggatgcg tgaaatccga gaaggcatag
     2401 agcaccttgt aggcgccgcc gcgctccttg agcttctcct tgatgatttc gaagtaccgc
     2461 aggccggcga ctcgcgaact ggtcacgatc atggccttgg cacggccgcc gatgagcggc
     2521 agcacgtcgg cctcaaagag gcgcagcatg acttcggcct tgtactgaat caggccgtca
     2581 tcctgaaaag ccacgttctg gagcgccttc gaaacgacgc tcttgggata gagcttttcc
     2641 tcatccggct tgggcaccac cgcgcaatgc aggttgtaga gcgtcttgta ggaaatgatg
     2701 ctggttgcga catcgacgat atagccctcg gcaatcgcct cttcttcggt gtaggtatcg
     2761 aaggcctcgc cgaacaggct caccgttgcc ggtgccggtg tcgccgtgaa agcgacgatc
     2821 atctgattga catcgtgctc acggatgatt ttggcgagtt gctcctgagg atcgggctct
     2881 cccggttcgt catcggctgc atcgggagcc tgggtccccg cgcggaacgg caggcggatg
     2941 gctgcaccca tctgcccttc ctgcgagcgg tgcgcctcgt cgatcaggaa ggccacgcgc
     3001 agcttcttga gctccggttt ctgctcgatc tgctccagca cccaggcaaa tttctgctgc
     3061 gtcgaaacaa tgatcggccg acgttccgcc atgaagcgcg gcaggtcctc cgctttccgc
     3121 gccaggccga tcacgtcctt gaggtgcgtg aaattctcga tctcgtcctt gatatgggta
     3181 tcgagcgact tgcggtcggt gaggatgaac accatgtcca ccagcttttc gctgctgcca
     3241 ggtttgaaca ggctatgcag acggtcggcc aaccagcaga tggtcagcgt cttgccgctg
     3301 cccgccgaat gattgaccag gtacttgcgg ccgatgtcgg cctggtcggc aaagcgtgcc
     3361 gaaatatcct ccgccacttt gcgcaccaaa cggctctggt gatagcgcgg gaacagggtg
     3421 aaggccgggt gctccggcac atcatcaacc gcgtcgcggt gcggcacgcg caccaggaaa
     3481 aatgaaagcg cttccagcaa gctgtctttc gccaacagct cgcgatagag aaattcaacc
     3541 ggatattcgc tgccgtcttg ggtcatcggc gcgttggtca gcccggtgtt gtgccaacga
     3601 aaattctgct cgcgacgcgg atccgtggcc gccttcacgt cgctggtatc ggcagcgagg
     3661 tagagaaacg gatggcagaa aatcctgtgc gtgtggtcac gcccggcgaa ctgcgtcacc
     3721 gcgtcatgca ccgtctggtt cttctcgtgc ttcaactcca gcgccacgat tggcaagccg
     3781 ttcaggaaca cgacgaagtc gatttcctgg ttggtttcgc caaaaaagaa gtgatgacga
     3841 aaggtgatgc ggttctcgcc gtatttcttg acggcggtac tctcggtgga acgtggcttg
     3901 gggtagaaca gccgaaactc cagcccacgc acctggagac cgccgcgcag cagcatccaa
     3961 agcggcgttt gctccagatt cttgcgaagc gccttgaata cttcgtagcg cgcatcgctg
     4021 ccgtagtcat tggtcagctt tttcagagta tccgcttgcg tggccttgag gaaagcccag
     4081 agctgatctt cagcgatgca gtgctcggtg tccgtgatgc cggattgttc gagcacgccg
     4141 taacgatgca cgcggatcag aaagttcgcg atgtgctgct gaaaggcgag ttccggcacc
     4201 ttttttgaca tatcacaccg ttggccactg ttccaaatgc tcaacccacg cccgaaaaaa
     4261 cggacattcc cgccggatgg catcgagccc gatttcgagc acagccaggg ttcccatcag
     4321 cggcttttca tagctcggca gcagattgat gatccgcttg gacggtgccg tcacgggcga
     4381 atcgttgatt tcctccggcg ttgcaaattg atcgcggatg gcctggaagg ccggcgacaa
     4441 gtccgctctc ccgatcccct ctccgagttt ggcggcatcg ctgaacagca agccctcgaa
     4501 ctcatgcatc atgacgtagg ggacaaaccg ttggtgatcg aagtccgctc ccatctttgc
     4561 gcaaatctct tcccgcaggg cgttttcgac cgtgccggcc ttttctgcaa acggccgcaa
     4621 gcccgcttcg gcgcgtcccg gccaagcccg ttcgccgctt tgaggcagcg cgtagtaatc
     4681 caccatcgtc gtcgagcggc atccggcgtc ttccttcaga tgccgcacga tgtcctggcg
     4741 tatcgaactc caggaccgga tgccgccgcg tcgatcccgc tggcgggcat tgccgaccag
     4801 ccgcgcgctg acggtagtgt acccatgctc gtacaggtgc gcggccagca cctcgttgac
     4861 gaaagtttct tccgtttcac cctcgacatg aacaagcagg cgcgtcatgg tctcactcaa
     4921 ctcccgggcg accgcccagc tcatttttct cccagagctg gcccaggctg taatcctgca
     4981 accacgagtc cagcctcgcc gggtccagtc gagtgaactg cgttccccca gccacccggt
     5041 cggcaaccag cacatcttcc ggctgaaagt ggtcgagcag gaacggcgac tgcgtggaga
     5101 tgatgacctg cgttttcgtc gaggcctgct tgatcagcga agccagcaag gtgatggcgt
     5161 agggatgcaa gcccaactcg ggttcatcga ccagaatcac cgacggtcga tagctctcgg
     5221 gctgcagcaa cagcgttgtc agggcgataa aacgcaaggt gccgtccgac aacgagggcg
     5281 catcgaaata ggcgtccgat cccttgtgcc gccactccag ccggattttg tccgggttca
     5341 gttgttgcgg ctcaagctga aaatcttcga agaacggcgc gacccgctgt accgtccgct
     5401 ggatcaggct gtatgacgcc gcgtgcttct cgcgcaggta atacagaaac gcggcgaggt
     5461 tcgatccgtc agggcgcaaa tagcggttgt cattgacgtc cgccgtcttc ttcatcggcg
     5521 acgtggagct ggtgtcgtgg aagtgataga ggcgccagcg gccgaggtgt tcgcgcacat
     5581 aatgcgccac ggggatggct ctctgcgcgc tgatgccggc ctccttgcct gcccgcgcaa
     5641 tcacctcgtt atagggctgc tccggatacc tcgccttgtc ccaaaaatat acgacctcag
     5701 agctgggaac gagctcgtcg gcatcggtcg gttcaaggtc gatgtcgtac tggttgcact
     5761 cgttccggaa ggcaatcctg atttgcagct tctgggtctg tttcgatccg aagtgcagca
     5821 ccttgtccgc cccgcccgcc ttgatcacat agtcctgcag gcgcccgtcg cgaatggcat
     5881 gcaaaaaggc gaacacgcca atgaaattgg acttgccgga accattcgac ccgatgacca
     5941 cgttgatcgc ccccagtttc aggttctcga ccgcagcgat gctcttgaag cctttgacgg
     6001 tgatggtttc gagttcaggc atgggtaggc tcctgctggc cg
//
LOCUS       DAOSIT010000066         6035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11844_length_6035_cov_9.481773, whole genome shotgun sequence.
ACCESSION   DAOSIT010000066 DAOSIT010000000
VERSION     DAOSIT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11844_length_6035_cov_9.481773"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLL14_02735"
     CDS             complement(<1..583)
                     /locus_tag="PLL14_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOG02866.1"
                     /translation="MQHANLITKLAEIVGTEQVLSSPEDMSAYLCDWRGRYRGNAICV
                     VRPASTAAVSAVVHTCAEAGVAMVPQGGNTSLCGAATPAPDGRAVLISLARLRRVRAI
                     DPANNTLTAEAGCVLQALREAAGAVGRLFPLSLAAEGSCQIGGNLSTNAGGVQVLRYG
                     NTRELTLGLEVVLPSGEIWDGLRGLRKDNTGYDL"
     gene            1014..1985
                     /gene="glk"
                     /locus_tag="PLL14_02740"
     CDS             1014..1985
                     /gene="glk"
                     /locus_tag="PLL14_02740"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOG02867.1"
                     /translation="MAMQVPATLVADIGGTRARFALLDDSGLPDLVRILSVDDFAGPL
                     EAIQAYLGELGRPVLRAAAIAVAGPVHHDEFQLTNAPWTFSRARLKSGLGLQRLLLLN
                     DFTALALSLPHLSVADRRQVGPGVPVELAPKAVLGPGTGLGVSGVLHDHGRWLALTGE
                     GGHCSLAPADEREAAILALAWRELGHVSAERLLSGSGLPLLHRLVAAVDGRHAASLAT
                     AEIVAHARTGDDIQCRVVIDTFCAMLGSLAGNLALTLGAQGGVYVGGGIIPRLGEIFD
                     RSAFRPRFESKGRFASYLAAIPTYVMLSPTPALLGAAHALAESEDCP"
     gene            2008..>2486
                     /locus_tag="PLL14_02745"
     CDS             2008..>2486
                     /locus_tag="PLL14_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG02868.1"
                     /translation="MSDVAQPHPKGEQLRAEIDRRLLRTVAAEPTRASKADLYQALSQ
                     VAREQLAQRWVDTQNADRGSKARRVYYLSMEFLIGRAMNNALSALGLRESAAEAFSAP
                     GPSLDEVMECEPDAALGNGGLGRLAACFLDSMATLGLPSWGYGVRYEYGMFAQSILNG
                     "
     assembly_gap    2487..2586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2587..4281
                     /locus_tag="PLL14_02750"
     CDS             <2587..4281
                     /locus_tag="PLL14_02750"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG02869.1"
                     /translation="AAEFKNRFENISWVLYPNDSTAAGRELRLRQEYFFVAASMQDIL
                     ERHMAEHGTFNNLAEHVAIHLNDTHPAIGVAELMRLLVDEQGMTWDAAWDQCRRIFSY
                     TNHTLMPEALETWKVTLIQRVLPRHILIIYRINQEFLDEVIRLYPGDVDLMRRVSLID
                     DGGGHDKDKRVRMAHLCIVGSHRVNGVSQLHSDLMVQTIFADFARLYPERFHNKTNGV
                     TPRRWLAQANPALSALLDERLGGKDWRLNLDRLEDLRASADDVAFRAAFAAAKRGNKV
                     RLANYVAREVGVTLDPDSLFDVQVKRIHEYKRQLLNVLHVITRYNALLDGSAKDYAPR
                     SVIFAGKAASSYHMAKQVIRLIHDVAAVVNNDPRTRDLLQVVFIPNYGVSVAELIMPA
                     ANLSEQISTAGTEASGTGNMKLSLNGALTIGTEDGANIEIRDNVGAENIFIFGNNTAQ
                     VNAIRQAGHQPLALYQADPALKEALDRIDSGFFSPGERPRYHDIFNSLLHYGDHYLLL
                     ADYADYVATQARVDALYLSPDEWQRKAILNVAGMGPFSADRTIRDYANDTWNLRTL"
     gene            4297..>6035
                     /gene="glgB"
                     /locus_tag="PLL14_02755"
     CDS             4297..>6035
                     /gene="glgB"
                     /locus_tag="PLL14_02755"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOG02870.1"
                     /translation="MLDHAEIIALCRAEHGDPYAVLGMHTDADGGLWVRSLQPGAHTV
                     AVIEARTGRQIAQLAQRPVEGVEGFFEGLIPRRRNSFAYRLRITWDSGVQETDDPYRF
                     PTVLGEMDVWLLAEGSHLRPFERLGAHLCEIDGVAGTVFAVWAPDAQRVSVVGDFNTW
                     DGRRHPMRLRRECGVWEIFLPGVNAGARYKYEIRARDGHLLPLKADPYGFQGEMRPST
                     ASIVCALPQPVEPAPVPAGEELGAPLSVYEVHLASWRRADDGGFPDWQRLTETLIPYV
                     VDLGFTHLELLPISEHPFDGSWGYQPLGLYAPTARHGDPSGFRHFVGSCHAAGLRVIV
                     DWVPAHFPTDEHGLGRFDGTALYEHADPREGMHQDWGTLIYNFGRREVSNYLIGNALF
                     WIERYGVDGLRVDAVASMLYRDYSREPGQWLPNVHGGRENLEAVAFLRRMNEVLGQEH
                     PSASTHAEESTAWPMVSRPPEMGGLGFHYKWNMGWMHDVLDYMTKDPVHRRFHHNQLS
                     FGMMYAFTENFILPLSHDEVVHGKGSLISKMAGDYWQKFANLRALYGYMWAHPGKKLL
                     FMGGEIGQWNEWN"
BASE COUNT         1035 a         1903 c         1860 g         1137 t
ORIGIN      
        1 tgaggtcata accggtattg tccttgcgca ggccgcgcaa gccatcccag atctctccgc
       61 tcggcagaac cacctcaaga ccgagcgtta actcgcgcgt gttgccgtaa cgcaggacct
      121 gcaccccacc ggcgttggtg gacagattgc caccgatctg acaactgccc tctgcggcca
      181 gcgacagcgg gaacagtcgt ccgaccgcgc cggctgcttc ccgcagggct tgcagcacgc
      241 agccggcctc ggcggtcagc gtgttgttcg ccgggtcgat ggcgcgcaca cgtctgagcc
      301 tggcaaggct gatcagcacc gcccgaccgt caggcgccgg cgttgccgcg ccacacaggc
      361 tggtattgcc accctgcggc accatcgcca cgccggcctc ggcgcaggtg tggaccaccg
      421 ccgagacggc ggcggtgctt gccggtcgga cgacgcagat ggcgttgcca cggtagcgac
      481 cgcgccagtc gcacaggtat gcggacatgt cttcgggcga cgacagcacc tgttccgtgc
      541 cgacaatttc ggcgagcttt gtgatcaggt tcgcatgttg cacaggcaat ccttgggcgt
      601 aaaaagacgg ccggcgggcg aggccgagcc ggagtccatt gtgggaaata ctgcacccgg
      661 ccagcggcgg gtctgccgcc tgtcgggcag taggggcagc gatggtctgc tcccgtcttg
      721 acagaacccg cgtcgaccga ggagactccc ggacggctga cagcatttcc ggagagggca
      781 ggcagatggt agcagggtct ttgataaatc tcgcatttgg tgcgaagatc ccgagcatcc
      841 tattccctat ccggccccag ggcattcaat cttgaacctc cagctccaag ctttcaaagc
      901 tatccgacgc ggctggcgac cggatgattc catgctccgc cgtggacggc acccggcccc
      961 gcgcgcggct ctcgactgcg cacctgcgcc gcgcaaccgc tgatgaactg gtgatggcca
     1021 tgcaggtacc cgcgaccctg gttgccgaca tcggcggcac ccgcgcccgt tttgccctgc
     1081 ttgacgactc gggattgccg gatctggtgc ggattctctc ggtggatgac tttgccgggc
     1141 ccctggaggc gatccaggcc tatctcgggg agttgggcag accggttctg cgggcagcgg
     1201 cgatcgctgt tgccggcccg gtgcatcacg atgagtttca actgaccaac gcgccgtgga
     1261 ccttctcgcg cgcaaggctc aagtccggac tggggctgca gcgcctgctg ttgctcaacg
     1321 atttcaccgc cctggcgctg tccttgccgc atctgagtgt tgccgaccgg cgtcaggtag
     1381 gtcctggcgt ccccgtcgag ttggcgccga aagcagtgct tgggccgggg accggcctcg
     1441 gcgtctcggg cgtgcttcac gaccatgggc gctggctggc cttgactggc gagggaggtc
     1501 actgctcgct ggcaccggcg gacgaacgcg aggcggccat tctcgccctc gcctggcgcg
     1561 agcttgggca cgtctcggcc gaacggctgc tttcgggcag cggtttgccg ctgctgcatc
     1621 gtctggtcgc cgcggtcgac gggcgccatg ccgcatccct ggcgactgcc gaaattgtcg
     1681 cgcatgcccg cactggcgac gacatccagt gccgagtggt gatcgatacc ttttgcgcga
     1741 tgctcggttc gttggcggga aatcttgcac tgacccttgg cgcccagggt ggcgtctatg
     1801 tcggcggtgg tatcattccc agactcgggg agatcttcga tcgctcggct tttcgtcccc
     1861 gcttcgaatc caaggggcgt ttcgcttcct acttggccgc aattccgacc tatgtaatgc
     1921 tttctcccac gccggccctg cttggcgctg cccatgccct tgccgagagc gaggattgtc
     1981 catgaacctt actggagtac cagcctgatg tccgatgtcg ctcaacccca tcccaaaggc
     2041 gaacaactgc gcgctgaaat cgaccgcaga ctgctgcgca cggtcgctgc cgagccaacc
     2101 agagccagca aggcggacct ttatcaggcg ctgtcgcagg tggcccgcga gcaactggcg
     2161 caacgctggg ttgacaccca gaatgccgac cgcggcagca aggcgcggcg cgtctattac
     2221 ctgtcgatgg aattcctgat cggccgcgca atgaacaacg ccctgtccgc gctggggctt
     2281 cgcgagtcgg cggcggaggc gttcagcgcg cctggtccct cgctcgatga ggtcatggaa
     2341 tgcgaacctg atgcagcgct cggcaacggc ggcctgggtc ggttggcggc ctgttttctc
     2401 gattcgatgg caaccctggg gcttccctcc tggggctacg gcgtccgcta tgagtatggg
     2461 atgttcgcgc agtcgattct caacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnngctg ccgagttcaa gaaccgcttc gaaaacattt cctgggtgct ctacccgaac
     2641 gacagcaccg ctgcgggtcg cgaactgcgt ctgcgccagg aatatttctt cgtcgcggcg
     2701 tcgatgcagg acatcctcga gcgccacatg gccgagcacg ggaccttcaa caatctcgcc
     2761 gagcatgtgg cgatccacct caacgatacg catccggcga tcggggttgc ggaattgatg
     2821 cgccttctgg tcgacgagca gggcatgacc tgggacgccg cctgggatca atgtcggcgc
     2881 attttctcgt acaccaatca caccctgatg ccggaggcac tggaaacctg gaaggtgacc
     2941 ctgatccagc gcgtgttgcc gcgtcacatc ctgatcatct accgcatcaa ccaggagttc
     3001 ctcgatgagg tgatccgtct ctaccccggt gatgtcgacc tgatgcgacg ggtctcgctg
     3061 atcgacgacg ggggtggtca cgacaaggac aagcgggtgc gcatggcaca cctgtgcatt
     3121 gtcggcagtc atcgcgtcaa tggcgtatca caactgcact ccgacctgat ggtacagacc
     3181 atttttgccg acttcgcccg tctctacccc gagcgattcc acaacaagac caatggcgtg
     3241 acgccgcgac gctggctggc acaggccaat cccgccctgt ccgcactgct cgacgaacgc
     3301 cttggcggca aggattggcg gctcaatctg gaccgtctgg aggatctgcg ggcgagcgcc
     3361 gacgacgtcg cttttcgcgc cgctttcgcg gcagccaagc gcggcaacaa ggtgcgcctg
     3421 gccaattacg tggctcgtga agtgggcgtc accctggacc cggacagcct gttcgatgtg
     3481 caggtcaagc gcattcacga atacaagcgg cagctgctca acgtgctgca cgtgatcacc
     3541 cgctacaacg cgcttctgga cggctcggca aaggattatg cgccgcgcag cgtgatcttc
     3601 gccggcaagg ccgcgtcgtc gtatcacatg gccaaacagg tgatccgcct gatccatgat
     3661 gtcgcggcgg tagtcaacaa cgacccgcgg acgcgcgacc tcctgcaggt cgtcttcatt
     3721 cccaactacg gcgtttccgt cgccgagttg atcatgccgg cggccaatct ctcggagcag
     3781 atttcgacgg ccggcaccga agcctcgggc accggcaaca tgaaactctc gctcaacggc
     3841 gcattgacca tcggtaccga ggatggcgcc aacatcgaga ttcgcgacaa cgtcggcgcc
     3901 gagaatattt tcatcttcgg caacaacacg gcccaggtca acgccatccg gcaggccggt
     3961 caccaaccac tggccctgta ccaggcggat cccgccctca aggaagcgct cgaccggatc
     4021 gacagtggct tcttttcgcc cggtgaacgg ccgcgctacc acgacatctt caacagcctg
     4081 ctgcactacg gcgatcacta tctgctgctg gcggactacg ccgattacgt ggccacccag
     4141 gcgcgtgtcg atgcgcttta cctgagcccc gacgagtggc agcgcaaggc cattctcaat
     4201 gtggccggaa tgggtccctt ctcggccgac cggacgatcc gcgactacgc caacgatacc
     4261 tggaatctac gcacactcta agtaaggaag tctcaaatgc ttgaccatgc agagatcatc
     4321 gccctttgcc gggcagaaca cggcgaccct tacgcggtcc tcggaatgca caccgacgct
     4381 gacggagggc tgtgggttcg cagcctgcaa ccgggcgcgc acacggtcgc cgtcatcgag
     4441 gccaggaccg ggcggcagat tgcccagctt gcgcaacgcc ctgtcgaggg cgtggaaggc
     4501 tttttcgagg gcctgattcc ccgccgtcgc aacagttttg cctatcgcct gcggattacc
     4561 tgggacagcg gcgtgcagga aaccgatgat ccctaccgct ttcccacggt gcttggcgaa
     4621 atggacgtct ggctactggc cgaaggctcg cacctgcgcc ccttcgaacg cctcggggcg
     4681 catctgtgtg agatcgacgg cgttgcgggt accgtctttg ccgtctgggc gccggatgcg
     4741 caacgcgtca gtgtcgtcgg cgacttcaat acctgggacg gtcgtcggca tccgatgcgc
     4801 ctgcggcggg aatgcggcgt ctgggagatc ttcctgccgg gggtaaacgc gggggcacgc
     4861 tacaagtacg aaatacgcgc cagggacggc catctcctgc cactcaaggc cgatccctac
     4921 ggcttccagg gcgagatgcg gccatccacg gcgtcgatcg tctgtgcgct gccgcaaccc
     4981 gtggagccgg cgccggtgcc ggcgggtgag gaactgggcg cgccgctttc ggtttatgag
     5041 gtgcatctgg cctcctggcg tcgcgcagac gatggcggct ttccggactg gcagcggctg
     5101 accgaaacgc tgattcccta tgtagtcgat ctgggattca cgcatctgga actgctgccg
     5161 atatcggagc accccttcga tggttcttgg ggttaccagc ccctggggct ttatgccccg
     5221 accgcgcgcc atggtgatcc gagcgggttc cgccatttcg tcggttcctg ccacgcggcg
     5281 ggtctgcgcg tcatcgtgga ctgggtgccc gcgcatttcc ccaccgacga gcatggtctc
     5341 ggtcgtttcg acggtacggc gctttatgag catgccgatc cacgcgaagg catgcatcag
     5401 gactggggca cgctgatcta taacttcggc cgtcgcgaag tcagtaatta tctgatcggc
     5461 aatgccttgt tctggatcga acgctacggg gtcgacggcc tgcgggtcga tgccgtggcc
     5521 tcgatgctgt atcgcgacta cagtcgtgag ccggggcagt ggctgccgaa cgtgcatggt
     5581 ggtcgggaga atctcgaagc ggtcgccttt ctgcggcgga tgaacgaggt cctgggtcag
     5641 gagcatccct cggcttccac ccatgccgag gagtcgaccg cctggccgat ggtcagtcga
     5701 ccgccggaaa tgggcggcct cggtttccac tacaaatgga acatgggctg gatgcacgac
     5761 gttctcgact acatgaccaa ggacccggtg caccggcgat tccaccacaa tcagctcagt
     5821 ttcggaatga tgtacgcttt caccgagaac ttcatcctgc cgctttcgca cgacgaagtc
     5881 gtgcatggca agggctcgct gatcagcaag atggccggcg attattggca gaaattcgcc
     5941 aacctgcgcg ccctttacgg ctacatgtgg gctcacccgg gcaagaagct gctgttcatg
     6001 ggcggcgaga tcggccagtg gaacgagtgg aatca
//
LOCUS       DAOSIT010000067         5972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11984_length_5972_cov_9.359304, whole genome shotgun sequence.
ACCESSION   DAOSIT010000067 DAOSIT010000000
VERSION     DAOSIT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11984_length_5972_cov_9.359304"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLL14_02760"
     CDS             <1..508
                     /locus_tag="PLL14_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOG02871.1"
                     /translation="LFDDHNVFRDRVNAALKTTGIKLAAADLKQILKAVSWRVDTAPP
                     VIARVHQPGKARAEPLHGLYAASVDGRPTVVEYEPDADLRDTEQVPLLEDGGIEAFIR
                     REVLPYTPDAWIREDATKIGYEISFTRHFYKPQPLRTLEEISADILAIEKEAEGLLDG
                     LLKVGAKP"
     gene            505..1641
                     /locus_tag="PLL14_02765"
     CDS             505..1641
                     /locus_tag="PLL14_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOG02872.1"
                     /translation="MKKKADPSARKPGPSALHSEQRRGLTAGKEAPAVVDAAGLLSDL
                     RSLIQSARQRIATVAYSTQTLLCWHVGRRLLNESLQGARAAYGKQILVTVSRELTAEY
                     GRGFSYAEIARMIQFAQLFPDEAIVVTLSQQLSWSHIHALLPLKDPLARDFYAEMCRI
                     ERWDVRTLRQKIGGLLFERTALSKKPQDVIAAEIGRLRDGQMSPDIVFRDPYLLDLLG
                     LKGAYSERDLESAILREIEGVLLELGSGFTFVARQKRISVGKDDFHLDLLFFHRHLRR
                     LIAVELKLESFQPGHVGQMELYLRWLDKHERAPGEEAPIGLILCASADAEQVELLQLD
                     AKSIRVSEYLTELPPLPLLRERLHQAIEHPRESAARRVVVEEQP"
     gene            1638..3011
                     /locus_tag="PLL14_02770"
     CDS             1638..3011
                     /locus_tag="PLL14_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOG02873.1"
                     /translation="MIADLKPYAEYKESGLSWLGQVPGHWELRRMKSAVMNVIEQRYD
                     LGPDEVYVALEHVESWTGKARPQSDSSLFSGQVKRFRKGDVLFGKLRPYLAKVTRINK
                     DGVCAGEFFVLRPTDSAVSSAYLEHQLRAKHTIDVINGSTFGAKMPRADWQFCGNLPF
                     LLPTPAEQAAIVRFLDWANGRLERAIRAKRKVIALLNEQKQAIIHRAVTRGLDPSVPL
                     KPSGIPWLGDIPQHWEVVRNMSLFAHRVEAGIAGLPVLQVSLRSGVTAEDLDPFGRPK
                     RLIADPTKYKLVRTSDMAYNTMRMWQGAVGVAPNDGLVSPAYVVLTPRQDTCPDFYDY
                     VFHTDVYKQQVNRQSTGIVSDRNRLYWDSFKQMPNIALARLEQETIVRFIHSETGTLI
                     AATARLQREIDLLREYRTRLVADVVTGKLDVREAAARLPDEAPLATDYGTDPSLDSEA
                     ADEESVA"
     gene            3008..4474
                     /locus_tag="PLL14_02775"
     CDS             3008..4474
                     /locus_tag="PLL14_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG02874.1"
                     /translation="MTTDRSVDYVAGLVRELCKLSGETEWLEFKVGNADPQEMGEYLS
                     ALANAAAYNGKAFAYLVWGVEDETHRVVGTCFSPFTSKRGNEPLETWLLRLLTPKIHF
                     HFSEVTLEGLPVVLLEIGRAFRHPVRFQNEAFIRIGSVKKPLKEAPDRERALWRIFDQ
                     TPFEDLVAVERASAEEVLRLLDYPAYFDLLERALPANREGILEALADDGLIKPSEAGG
                     WNITNLGAILFAKRLEEFRALRRKALRVIQYRGAGRVETIREQVIGKGYANGFEGLMD
                     YVNGLLPSTEVVGQALRRTVPKFPPLAVRELVANGLIHQDFFVTGAGPMVEIFDDRIE
                     ITNPGEPLVDTQRFVDTPPKSRNEALASLMRRFRFCEERGSGIDKVVAQIELLQLPAP
                     LFEAPEGFTRIVLFAHRPLKEMDKADRIRACYLHACLKWVLRGQMTNASLRERFGIQE
                     KNKATASRLIREALDAGVIRLVNDDAGDRFRAYLPFWA"
     gene            4607..>5972
                     /locus_tag="PLL14_02780"
     CDS             4607..>5972
                     /locus_tag="PLL14_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOG02875.1"
                     /translation="MTTDTSEKGLETLIMRHMTGVDGLAIAPNSVARPQAPYGGTGYI
                     AGSAQDYDRAHALDVPQLFAFLRATQPDAFRKLALADANDASDINRLKFLARLSAEIG
                     KRGVIDVLRKGVAHGPLHFALFYGTASKGNARAEARHAQNRFSITRQLAYSIDETRRA
                     LDLCLFINGLPIATFELKNRLTKQTVEDAVEQYRRDRNPRERLFEFGRCVVHFAVDES
                     EVQMCTELRGRGSWFLPFNKGYHDGAGNPPNPHGLKTDYLWKEVLTPAGLTNILENYA
                     QIVEEKDPRTGRKKRRQVWPRYHQLGVVRQALADVRAHGAGKRYLIQHSAGSGKSNSI
                     AWLAHQLIGLQRDGDQAKGTNREVFDSVIVVTDRRLLDDQIQTTIKQFMQVGATVGHA
                     ERSGDLRRFIAQGRKIIVSTVQKFPFILDEIATESGRTFAIIIDEAHSSQGGKTSAAM
                     SQALG"
BASE COUNT         1145 a         1877 c         1896 g         1054 t
ORIGIN      
        1 gctcttcgac gaccacaacg tcttccgcga ccgcgtgaat gcggccttga agaccaccgg
       61 gatcaaactc gccgccgccg acctgaagca gatcctcaag gccgtgagct ggcgcgtcga
      121 taccgcgccg ccggtcatcg ccagggtgca ccagcccggc aaggccagag ccgagccgct
      181 gcatggtctc tacgcggcga gcgtggacgg caggcccacc gtcgtcgagt atgagcccga
      241 cgccgacctg cgcgataccg agcaggtgcc gctgctcgaa gacggcggca tcgaagcctt
      301 catccgccgc gaggtgctgc cgtacacgcc cgacgcgtgg atcagggaag acgccaccaa
      361 gatcggctac gagatcagct tcacgcgcca cttctacaag ccgcagccgc tgcgcacgct
      421 cgaagagatc agcgccgaca tcctggccat cgagaaggag gccgagggct tgctggatgg
      481 tttgctcaag gtgggcgcca agccatgaag aagaaagccg acccttcagc cagaaagccc
      541 ggaccctccg cgctacactc cgagcagcgc cgcggcctga ccgccggaaa ggaagcgccc
      601 gcagtcgttg atgcggcggg cctcctctcg gacttgcgca gcctcatcca gtccgcacgc
      661 cagcggattg ccaccgtcgc ctattccacc cagacattgc tctgttggca tgtgggacga
      721 cggctcttga acgagagtct acagggcgct cgggctgcct atggaaagca gattctcgtg
      781 accgtgtcac gagaattgac ggcggagtac gggcgcgggt tcagctatgc cgagattgcc
      841 cgcatgatcc agttcgcaca gttgttcccc gatgaggcga ttgtcgtgac gctgtcacaa
      901 caattgagct ggtcgcatat tcatgcgctg ctgccgctca aggatccgct ggcccgcgat
      961 ttctacgccg agatgtgccg catcgagcgt tgggacgtgc gcacgctgcg ccagaagatc
     1021 ggcggcctgc tgttcgagcg caccgcactg tcgaagaagc cgcaagacgt catcgcggcc
     1081 gagatcggca gacttcgcga cgggcagatg agccccgaca tcgtctttcg cgatccctac
     1141 ctgctcgacc tgctggggct caagggtgcc tacagcgaac gcgacctcga gagcgccatc
     1201 ctgcgcgaga tcgagggtgt gctgctggaa ctgggcagcg gcttcacctt cgtcgcccgg
     1261 caaaagcgca tcagcgtcgg caaggacgat tttcatctcg acctgctttt cttccaccgt
     1321 catctgcgcc ggctgatcgc cgtcgaactg aagctggaat ccttccagcc gggccatgtc
     1381 gggcagatgg agctatacct gcgctggctg gacaagcacg aacgtgctcc cggcgaggaa
     1441 gccccaatcg gactcatcct ctgcgcctcc gccgacgccg agcaggtgga actgctgcag
     1501 ctcgatgcca aatcgatccg cgtcagcgag tacctgacgg agttgccgcc actgccactg
     1561 ctgcgcgagc ggctgcatca ggcgatcgaa cacccgcgcg agagcgccgc ccggcgagtg
     1621 gtggtggagg aacagccatg atcgccgacc tcaagcccta tgcggagtac aaggagtcgg
     1681 ggttgtcttg gctggggcag gtgccggggc attgggagct gcggcggatg aaatcggccg
     1741 tcatgaacgt catcgaacag cgttacgact tgggtcccga cgaggtctac gtcgcgctgg
     1801 agcacgttga gagttggact ggcaaggcac gtccccagtc ggattcgagc ctcttttcag
     1861 gacaagtaaa gcggttccgg aaaggcgacg tgctgttcgg gaagttgcgt ccgtacttgg
     1921 ccaaggtcac tcgtattaac aaagacggcg tgtgtgcagg cgagttcttc gttctgcgtc
     1981 ctaccgacag cgcggtgagt tcggcgtact tagaacatca acttcgggcg aagcacacaa
     2041 tcgatgtcat caacggatca acctttggcg cgaagatgcc tcgtgcagat tggcagttct
     2101 gcggcaacct accttttctc ctccctacac ccgctgagca agccgcgatt gtgcggttcc
     2161 tggactgggc gaacgggcgg ttggagcggg cgatccgggc gaagcggaag gtgatcgcgc
     2221 tgctcaacga gcagaagcag gccatcatcc accgcgccgt cacccgcggc ctcgacccct
     2281 ccgtccccct caaaccctcc ggcatcccct ggctcggcga cattccgcag cattgggagg
     2341 ttgtccgaaa catgagtctt ttcgcccatc gggtagaagc aggaatagcg ggccttcccg
     2401 ttcttcaggt tagtctccgt tctggcgtca cagccgagga cttggacccg tttggacgcc
     2461 ccaagaggct gattgccgac ccaaccaaat acaagctggt ccggacgtca gacatggcct
     2521 acaacaccat gcggatgtgg cagggtgcag ttggcgttgc accaaacgat ggtctcgtga
     2581 gcccagcgta tgtggttctg acgccccgcc aagacacatg ccccgacttc tacgactacg
     2641 tattccacac cgacgtttat aagcaacagg tcaatcggca gtccaccggc atcgtgtcag
     2701 accgtaaccg gctttactgg gatagcttca agcaaatgcc aaacatcgct cttgctcggc
     2761 tagagcagga aacaatcgtt cgcttcattc actccgaaac cgggactctc atagccgcca
     2821 ccgcccgcct ccaacgcgag atcgacctcc tccgcgaata ccgcacccgc ctcgtcgccg
     2881 atgtcgtcac cggcaagctc gacgtgcgcg aggcggcagc acggctacca gacgaagcac
     2941 cgcttgccac cgactacggc accgatccga gccttgattc cgaagccgcg gacgaagagt
     3001 ccgtcgcatg accaccgacc gcagcgtcga ctacgtggct gggctggtcc gcgagttgtg
     3061 caagttgtcg ggcgagaccg aatggttgga gttcaaggtc ggcaacgccg atccgcagga
     3121 gatgggcgag tatctgtcgg cactggcgaa cgcggcggca tacaacggca aggccttcgc
     3181 ttatctggtc tggggcgtcg aggacgagac ccatcgcgtc gtgggtacgt gcttttcacc
     3241 cttcacgagc aagcggggca acgagccgct ggagacctgg ctgctgcgct tactcacgcc
     3301 gaaaattcac ttccatttct cggaggtgac gctggagggg ctgccggtgg tgctgctgga
     3361 gatcggccgg gcctttcgtc atccggttcg cttccagaat gaggccttca ttcgcattgg
     3421 ttcggtcaag aagccactga aggaggcgcc cgaccgcgag cgcgcactgt ggcgaatctt
     3481 cgaccagacg cccttcgagg acctggtcgc ggtcgaacgg gccagtgccg aagaggtcct
     3541 gcgccttctc gactacccag cctacttcga cctgctcgaa cgggcgctgc cggccaatcg
     3601 cgaaggcatc ctcgaggctc tggccgatga tggcctgatc aagcccagcg aggccggggg
     3661 ttggaacatc accaacctgg gagcgatcct gttcgccaag cggctggagg agttccgggc
     3721 gctgcgacgc aaggccctgc gggtgatcca gtatcggggc gcgggccgtg tcgagacgat
     3781 ccgggagcag gtgatcggca agggctacgc caacggcttc gaggggttga tggactatgt
     3841 gaacggcctg ctgccatcca cggaggtcgt ggggcaggcc cttcgccgga cggtgccgaa
     3901 gtttccgccg ctggcggttc gggagctggt ggccaatggg ctgatccatc aggacttctt
     3961 cgtcaccggg gcgggaccga tggtggagat cttcgatgac cgcatcgaga tcaccaaccc
     4021 cggcgaaccg ctggtcgata cccagcgctt cgtggacacg ccgccgaagt cgcgcaacga
     4081 ggccttggca tcgctgatgc ggcggtttcg gttctgtgaa gaacgcggca gtgggatcga
     4141 taaggtggta gcgcagatcg agctgttgca gttgccggcg ccgttgtttg aggccccgga
     4201 agggtttacg aggatcgtcc tgtttgcgca ccgaccgctg aaggaaatgg ataaggctga
     4261 tcgcatccgc gcctgctatc ttcacgcttg tctgaagtgg gtgctccgcg gccagatgac
     4321 caacgcatcc ttgagggagc ggttcgggat ccaggagaag aacaaggcca ccgcctcgcg
     4381 gctgattcgc gaggctttgg atgctggcgt gatccgtttg gttaacgatg atgctggtga
     4441 caggttccgc gcctatctgc cgttttgggc ctgagtcttg ttgatgggtg gttgatgggc
     4501 gccacaccgc tgactgtcgc caatccgata tgcctataaa aatcagaacg atgaaccgca
     4561 tggttctggt tgatgggtcg ttgataaagt gcaaaggagg aggcgattga ccaccgacac
     4621 cagcgagaag ggcctcgaaa cgctgatcat gcggcacatg actggcgtcg atggcttggc
     4681 catcgcgccg aacagtgtcg ctcgaccaca ggcaccttat ggcggcaccg gctacattgc
     4741 cggcagcgcg caggactacg accgcgcgca tgcgctcgac gtgccgcagc tcttcgcctt
     4801 cctgcgcgcc acccagcccg acgccttcag gaagctggca ctggccgacg cgaacgacgc
     4861 cagcgacatc aaccgcctca agttccttgc ccgcctttcg gccgagatcg gcaaacgtgg
     4921 cgtcatcgac gtgctgcgca agggcgtggc gcacgggccg ttgcactttg ccctgttcta
     4981 cggcacggcc tcgaaaggca atgcccgggc cgaggcgcgg catgcgcaga accgcttctc
     5041 gatcacgcgc cagctcgcct acagcatcga cgagacgcgc cgcgcgctcg atctctgcct
     5101 gttcatcaac ggcctgccga tcgccacttt cgagctgaag aaccgtctca ccaaacagac
     5161 ggtcgaggat gccgtcgagc agtaccggcg cgaccgcaat ccgcgcgaac ggctgttcga
     5221 gttcggccgc tgtgtggtgc acttcgcggt cgacgagagc gaggtgcaga tgtgcaccga
     5281 gctgcgcggc aggggctcgt ggttcctgcc gttcaacaag ggttatcacg acggcgccgg
     5341 caacccgccc aacccgcacg gcctgaagac cgactacctc tggaaggagg tgctcacccc
     5401 ggcgggtctg acgaacatcc tcgagaacta cgcgcagatc gtcgaggaga aggatccgcg
     5461 cacgggcagg aagaagcgcc ggcaggtctg gccgcgttat caccagctcg gcgtcgtgcg
     5521 ccaggcgctg gccgatgtgc gcgcgcatgg ggcgggcaag cgctacctga tccagcactc
     5581 ggcgggcagc ggcaagtcga attcgatcgc ctggctggcg caccagctca tcggcctgca
     5641 gcgcgatggc gatcaggcga aggggacgaa cagggaggtt ttcgactcgg tgatcgtggt
     5701 caccgaccgg cgcctgctcg acgaccagat ccagacgacg atcaagcagt tcatgcaggt
     5761 cggcgcgacg gtcgggcacg ccgagcgctc gggcgacctg cgccgcttca tcgcacaagg
     5821 ccggaagatc atcgtctcga cggtgcagaa attccccttc atcctcgacg agatcgccac
     5881 cgagagcggc aggaccttcg ccatcatcat cgacgaggcg cattcgagcc agggcggcaa
     5941 gacctcggcg gcgatgagcc aggcgctcgg cg
//
LOCUS       DAOSIT010000068         5971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_11989_length_5971_cov_8.609533, whole genome shotgun sequence.
ACCESSION   DAOSIT010000068 DAOSIT010000000
VERSION     DAOSIT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11989_length_5971_cov_8.609533"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="PLL14_02785"
     CDS             complement(<1..151)
                     /locus_tag="PLL14_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HOG02876.1"
                     /translation="MLSFGDGTMFRISQYMQELTNPRPPGPKVNPPAPVVIWNLIRRC
                     NLTCKH"
     gene            310..891
                     /locus_tag="PLL14_02790"
     CDS             310..891
                     /locus_tag="PLL14_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HOG02877.1"
                     /translation="MMLLLLGLVIFFGTHSTRIFAENWRTWQVERLGEQRWKTIFSLA
                     SLLGFALLVWGYGQSRSDPVVLWLPPVWTRHLAALLTVPAFVLVAAAYVPGNRIRRMV
                     GHPMVAGTAVWAVAHLLTNGNLADVLLFGAFLCWAVADFAAARRRDRGRSPSPLVEAR
                     LSGDVWVLVVGLTAWNAFAWFAHAWLIGVAPFA"
     gene            983..1804
                     /locus_tag="PLL14_02795"
     CDS             983..1804
                     /locus_tag="PLL14_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG02878.1"
                     /translation="MNAQDVTRWQDMALQYGSELGVKVLAAIAFWVIGRWLIHQVGRI
                     VQGTLERQKVDATLMRYLGTVINVTLNILLVIGILGYFGIQTTTFAALFAAAGVAIGM
                     AWSGLLANFAGGVFLIILRPMKVGDFVSVAGVTGTVKEVGLFTTAINTPDNVLTLVGN
                     NKIFSDTIQNFSINAYRRVELKAQLSGAADHAAAMALLKEKIGLVPNVLATPPVEVEI
                     LEFNLVGPVLAVRPYCHNDHYWQVYFDTNRTIREALGAAGFPAPMPAQVVFVNQQ"
     gene            2036..>2216
                     /locus_tag="PLL14_02800"
     CDS             2036..>2216
                     /locus_tag="PLL14_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HOG02879.1"
                     /translation="MVAKFLPGNRLTLLNSGAEYFPALITAINEAKRHVHLESYIFED
                     DATGRAVAAAMANAAR"
     assembly_gap    2217..2316
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2317..3351
                     /gene="clsB"
                     /locus_tag="PLL14_02805"
     CDS             <2317..3351
                     /gene="clsB"
                     /locus_tag="PLL14_02805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HOG02880.1"
                     /translation="EDDATGRAVAAAMANAARRGVTVRVLVDGFGAREFADRLQPGLV
                     AAGVQAMIYRPDIARFHLRRHRLRRLHRKLVVIDGEIAFVGGINIIDDLNSPHHLPPR
                     YDYAVRVEGPLLAPIQLALHRLWELVLWAKLNRRYRLSRISYKEPEPRGHQTAAFLVR
                     DNIRHRRDIEEAYLQAIAAAEGEIILANAYFLPGRRFRRALQDAVKRGVQVVIVLQGR
                     VEYRLLHYATQALYGKLLGAGIRIFEYQRSFLHAKVAVIDRHWATVGSSNIDPFSLLL
                     AREANVVVRDEKFAGELRHSLRQAMRNGAHELPVDSWKRLPWHSRLLRWGSYNLVRTL
                     VGITGYGGKR"
     gene            complement(3438..4376)
                     /gene="gluQRS"
                     /locus_tag="PLL14_02810"
     CDS             complement(3438..4376)
                     /gene="gluQRS"
                     /locus_tag="PLL14_02810"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOG02881.1"
                     /translation="MSLAPLLDGSDGGIYRGRFAPSPTGPLHFGSLVTAVGSYLDARA
                     NAGKWLLRIEDVDQPRTVPGATDTILRTLESFGFEWDGQVLFQSRRLDHYHAALSRLQ
                     LSGATYPCTCSRREIAMCSPSPSVDGGLVYPGICRAGQGAGRATRAWRMRVPDEVIAV
                     ADRVQGTVQQNLESAVGDFVLMRADGQFAYQLAVVVDDAAQGINAIVRGADLLDSTPR
                     QVWLQQRLGLPTPAYAHLPVVNNATGEKLSKQTRAAAVPPSGGSALLSAAVHFLGHPV
                     PGEMVGAPVPEFWDWAIANWSIERVPPLRGAFPGRR"
     gene            4502..5389
                     /gene="argB"
                     /locus_tag="PLL14_02815"
     CDS             4502..5389
                     /gene="argB"
                     /locus_tag="PLL14_02815"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOG02882.1"
                     /translation="MTDRSITPANAAAILAEALPYIKRFHGKTIVVKYGGNAMTDENL
                     KQCFARDVVLLKLVGMNVVVVHGGGPQIENLLKRVGKKGRFVQGMRVTDAETMEIVEM
                     VLGGQVNKDVVNLINQAGGKAVGLTGKDGSFIRAKKLLLANQDDSDDLIDVGQVGDIM
                     QIDPSLIGHLEAGGFIPVVAPIGVGKGGETYNINADVVAGKIAEILKAEKLVLLTNTP
                     GVLDEAGSLITGMTPKQIDDMVANGTLSGGMLPKISSALDAARNGVKSVHIIDGRVEH
                     ALLLEILTDHGVGTMIKSH"
     gene            5400..>5971
                     /locus_tag="PLL14_02820"
     CDS             5400..>5971
                     /locus_tag="PLL14_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOG02883.1"
                     /translation="MLKAASPVWLFDLDNTLHDAGPHIFPHINRSMVEYIRDHLGIEE
                     EEATRIRQVYWQRYGATLLGMMRHHGTDARHFLWHTHQFPDLKHMLVIERGLRALLRR
                     LPGRKLVFSNAPLCYSQKVLGLAGISACFDAVYSIERIRFQPKPAVGGFRFLLRSESL
                     SAHRCIMVEDALSNLWTAKQLGMKTVWVSTA"
BASE COUNT         1071 a         1745 c         1876 g         1179 t
ORIGIN      
        1 aatgcttgca ggtcagattg cagcgccgga tcaggttcca gatcaccacc ggcgctggcg
       61 ggttcacttt cgggccaggt ggtctggggt tcgtgagttc ctgcatgtat tgcgagatcc
      121 gaaacatcgt gccatccccg aaagaaagca gtccattaga aatcaggcca tgggggatgg
      181 ccttgacgtg gatcaagggc gtgcatgcac ggtgagcagg actgtccgct gctcgccggc
      241 gaagccgggg cttctccgca actctgccgc gcgctattct tgcacgtgta tggtttgcgg
      301 ggaacgctca tgatgttatt gctgcttggt ctggtcatct tcttcggtac tcactcgacg
      361 cgaatcttcg ccgagaactg gcgtacttgg caggtcgaga gactaggtga gcaacgctgg
      421 aagacgatct tttcgctggc ttcgctgctc ggtttcgcgc tgctcgtctg gggctatgga
      481 cagagccgat ccgatccggt ggtattgtgg ctgccgcccg tatggacacg ccatctggct
      541 gcgctgctca cggttccggc tttcgtgctg gtggccgcgg cgtatgtgcc cggcaaccgc
      601 atcaggcgaa tggtcgggca tccgatggtc gccggtacgg ccgtctgggc tgtcgcgcac
      661 ctgttgacga acggcaatct ggcggacgtg ctgctgtttg gtgcctttct atgctgggcg
      721 gtcgctgatt ttgccgccgc gcgtcggcgt gatcgcggcc gcagtccgag tccacttgtc
      781 gaggcgcggt tgtcaggcga cgtctgggtc ctggttgtcg gtctcaccgc gtggaatgcc
      841 ttcgcctggt ttgcgcatgc ctggctgatt ggcgtcgctc ccttcgcctg attactgttc
      901 cgctgccggc agcaagggct tgcgctgccc atagcacaat gggaaactgc tcccaccctc
      961 aataaaccca ctggagaaac acatgaatgc acaagacgtg acgcgctggc aggacatggc
     1021 gttgcaatac ggctcggaat tgggcgtcaa ggtacttgcg gcaattgcct tctgggtcat
     1081 cggtcgctgg ctgattcatc aggtcggccg catcgtccag ggcacgctgg agcgtcagaa
     1141 ggtcgatgcc acgttgatgc gctacctggg cacggtcatc aatgtcaccc tgaacatcct
     1201 gctggtgatc ggcatcctgg gctatttcgg aattcagacc accaccttcg ccgcactctt
     1261 tgccgctgcc ggtgtggcca tcggcatggc ctggtccggg ttgctggcca actttgccgg
     1321 cggggtgttc ctgatcatcc tgcggccgat gaaggttggc gatttcgtct cggttgccgg
     1381 cgttacgggc accgtcaagg aggtgggtct gtttaccacg gcgatcaaca cgcccgacaa
     1441 cgtgcttacc ctggtcggca acaacaagat cttctcggac accatccaga acttctcgat
     1501 caatgcctac cggcgcgtcg aactgaaggc ccagttgtcc ggcgcagccg accacgcggc
     1561 ggcaatggca ctgctcaagg agaaaatcgg gctcgttccg aacgtgctgg cgaccccgcc
     1621 ggtggaggtc gagattctcg aattcaatct tgtcgggccg gtgcttgccg tacgtcccta
     1681 ctgccacaac gatcactact ggcaggtcta tttcgacacc aaccggacca ttcgtgaagc
     1741 actcggagcg gcgggcttcc cggcaccgat gccggcgcag gtggtgtttg tcaatcagca
     1801 gtagcgtcag ggatttgcgc cgcgagagtc ggggacagcg agtaaccagg ctgagacgca
     1861 ggggattctc gtggcaggtg gcccggatga tgcaccggcg ggtttcagcc ccttttccaa
     1921 tgtctcagga gggacgctgt tcgccagcca gaacgcaacc gggagggcat cgcaggctta
     1981 gcggttccgt atcgatggca gtcgggttct ggctgtctgc aggcgtggcg gcgtgatggt
     2041 tgccaagttc cttcctggta accgtctgac gctactgaac agtggcgcgg aatattttcc
     2101 ggcactgatt acggcgatca acgaggcgaa gcgacacgta catctagaga gttacatctt
     2161 tgaggacgac gccacgggtc gtgccgtcgc cgcagcgatg gccaatgccg cgcgtcnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngagg acgacgccac gggtcgtgcc
     2341 gtcgccgcag cgatggccaa tgccgcgcgt cgcggtgtca cggttcgcgt cctcgtcgac
     2401 ggtttcggtg cccgcgagtt tgccgatcgg ttgcaacctg gactggtggc ggccggtgtt
     2461 caggcaatga tctaccgacc cgacatcgcc cgctttcatt tgcgtcgcca tcgtctccgg
     2521 cgtttgcacc gcaagctggt ggtgatcgat ggtgaaattg ctttcgtcgg tggcatcaat
     2581 atcattgacg atctgaactc cccgcatcac ctgccgccgc gctacgacta cgcggtgcgc
     2641 gtcgaagggc cgttgctggc gccgatccag ttggcgttgc accgcttgtg ggagttggtc
     2701 ctgtgggcaa aactcaatcg ccgctaccgc ctgagcagaa tctcgtacaa ggaaccggag
     2761 ccgcgcgggc accaaacggc ggctttcctg gtgcgcgaca atatccgtca ccggcgcgac
     2821 atcgaggagg cgtacctgca ggccattgcc gccgccgagg gcgagatcat tctggccaac
     2881 gcctacttcc tgccgggccg gcgcttccgg cgcgccctgc aggatgccgt gaaacgtggt
     2941 gtgcaggtgg tcatcgtgtt gcaggggcgt gtggaatacc gtttgctgca ttacgccaca
     3001 caggcgctct acggcaaact gctgggtgcc gggatccgca ttttcgagta tcagcgcagc
     3061 ttcctgcacg ccaaagtcgc ggtcatcgat cgccactggg cgactgtcgg ctcgtcgaac
     3121 atcgatccct tcagcctgct gctggccagg gaagccaatg tggtcgtgcg cgacgagaag
     3181 ttcgccggcg aactgcggca cagtcttcgc caggcgatgc gcaatggcgc ccatgagttg
     3241 ccggtcgata gctggaagcg tttgccctgg cattcccgcc tgctgcgctg gggtagctac
     3301 aatctggtcc gcacgctcgt gggcattacc ggttacggcg gcaagcgttg aggctaagcc
     3361 agcaatgatt cctgcatgag cgcgtcgaag ccaggacgac ggccaggcag gtggtaagcg
     3421 tgaagccatg cagcacatca ccgtcgacca ggaaaggcac cgcgcagagg tggaacgcgt
     3481 tcgatggacc agttggcaat cgcccagtcc cagaactcgg ggaccggtgc gccaaccatc
     3541 tcgcctggca ccgggtggcc cagaaagtgt actgctgccg agagtagcgc actgccaccc
     3601 gaggggggca cggccgctgc gcgcgtctgc ttggagagtt tttcaccagt ggcattgttg
     3661 accaccggca ggtgggcgta agccggtgtc ggcagtccca gtcgttgctg caaccacacc
     3721 tggcgtggcg tcgagtcgag caggtcggcg ccacggacga tggcgttgat gccctgcgca
     3781 gcgtcgtcga cgaccaccgc caactggtac gcgaactgac catcagcgcg catcaggacg
     3841 aaatccccta cagcactttc aaggttctgc tgcaccgttc cctgtacgcg atcggcaacg
     3901 gcgatgacct catctggaac gcgcatccgc caggcgcgtg tcgctcgccc ggcgccctga
     3961 ccggcgcggc agatgccggg gtacaccagc ccgccgtcca ctgacggcga gggtgagcac
     4021 atggcgatct cgcgtcggga gcaggtgcag ggataagtcg cgccgctgag ttgcagtcgg
     4081 ctcaacgccg catgatagtg gtccagccgc cggctctgga acaggacctg gccatcccat
     4141 tcaaagccaa aggattcgag ggtccgcagg atcgtgtcgg tggcgcccgg cactgtccga
     4201 ggctggtcga cgtcctcgat gcgcaagagc catttgccgg cattggcgcg ggcgtcaagg
     4261 taactgccga ctgccgttac cagcgaaccg aagtggagtg ggccggtcgg tgagggcgca
     4321 aagcgaccgc ggtagatccc gccatcgctt ccgtcaagca acggtgcaag gctcatggtt
     4381 actccagtcg tggaggctgg cgggtgcttt ggcggcaact ttattgacca gcgcggcgag
     4441 ccgcctttgt atttccagcc gcgccgagta gaatccgccg gtcactatca ggactgccgc
     4501 catgactgat cgttcgatca cccctgccaa tgctgccgcc attctcgccg aggcactccc
     4561 gtacatcaaa cggtttcacg gcaagaccat cgtcgtcaag tatggcggca atgccatgac
     4621 cgacgagaat ctcaagcagt gcttcgcgcg tgacgtcgtg ctcctgaagc tcgtcgggat
     4681 gaacgtggtg gtggtccacg gcggtgggcc gcaaatcgaa aacctgctca aacgtgtcgg
     4741 caagaaaggg cgattcgtgc agggaatgcg cgtcaccgat gcggaaacga tggaaatcgt
     4801 cgagatggtg ctgggtggtc aggtgaacaa ggatgtggtc aacctgatca accaggccgg
     4861 aggcaaggcg gtcggcctga ccggcaagga cggcagcttc attcgcgcca agaaactgct
     4921 gttggccaat caggacgaca gcgatgacct gatcgacgtt ggccaggtcg gagacatcat
     4981 gcagatcgac ccgtcattga tcgggcatct ggaggcagga ggttttatcc cggtggtggc
     5041 gccgatcggt gtcggcaagg gcggtgaaac ctacaatatc aacgccgacg tggtggccgg
     5101 caagattgcc gaaattctca aggccgagaa gctggtcctg ctgaccaata cgcccggcgt
     5161 cctcgacgaa gcgggttcgc tgatcaccgg gatgacgcca aaacagatcg atgacatggt
     5221 cgccaacggg acactgtccg gaggcatgct accaaagatc agttcggcgc tcgatgccgc
     5281 gcgcaacggc gtcaagagtg tgcatatcat cgatggtcgt gtcgagcacg cgctgttgct
     5341 ggaaatcctg accgaccacg gggtcggcac gatgatcaag tcgcactgat cgcctgatag
     5401 tgctgaaagc cgcgtcaccg gtctggcttt ttgacctcga caatacgctg cacgacgccg
     5461 gcccgcatat ctttccgcac atcaatcgct cgatggtcga gtacattcgc gaccatctcg
     5521 gtattgagga agaggaagcg acgcgtatcc ggcaagtcta ctggcagcgc tacggtgcga
     5581 ccctgctggg catgatgcgt catcacggca ccgatgcccg gcacttcctg tggcacactc
     5641 accagttccc cgatctcaag cacatgctgg tcatcgagcg tgggctcaga gccctgctgc
     5701 gtcgcctgcc ggggcgcaag ctggtcttct cgaacgcgcc cctgtgttat agtcaaaagg
     5761 ttctcggact ggccggcatc agtgcctgtt tcgacgcggt ctattcgatc gaacgcattc
     5821 gctttcaacc caagccggca gttggcggct ttcgtttcct gctgcgtagc gaaagcctgt
     5881 cggcgcatcg ctgcatcatg gttgaagacg cgttgtccaa cctgtggacc gccaagcagt
     5941 tggggatgaa gacggtgtgg gtgagcaccg c
//
LOCUS       DAOSIT010000069         5959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12017_length_5959_cov_7.775407, whole genome shotgun sequence.
ACCESSION   DAOSIT010000069 DAOSIT010000000
VERSION     DAOSIT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_5959_cov_7.775407"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLL14_02825"
     CDS             complement(<1..613)
                     /locus_tag="PLL14_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HOG02884.1"
                     /translation="MKTFSLLTAILAFALTLAIGDAEAASKRFGAGKSSGMQRETTAE
                     RSLTTTPAPASATSAAKAPSPAAAPSAQPKRSWMGPLAGLAAGLGLAALASHFGFGEE
                     LASMLMIGLLVMAVVLVIGFVMRRRAGTQRPATDETGGLQYAAVGADGLPPNRGAHEL
                     ALPASGAAATGSSTDSHIPEDFDAAGFVRNAKLSFIRLQAANDH"
     gene            complement(830..1849)
                     /gene="nhaR"
                     /locus_tag="PLL14_02830"
     CDS             complement(830..1849)
                     /gene="nhaR"
                     /locus_tag="PLL14_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HOG02885.1"
                     /translation="MAALNFKHLRYFWVVAKSGAIARAGEQLHLTPQSISGQIRELEE
                     ALGVALFRRVGRGLELTDSGRRILGYAEEIFALGDELLDAVRDQTATKSLPFAVGIAD
                     SVPKSVAHRLVEPALHLPEPVRLICREGRLTALLAELAIHRLDMVIADRPMPANLNVR
                     GYNHLLGESTLTVFGAAALVETLRPNDSFPAMLDGAPFLMPGEDVAIRPRLEHWFEAQ
                     RLHPRIIGEFDDSALLKAFGQAGDGLFVAPTAIAAYVCEQYRVTSLGRIDSIAEQLFA
                     ITTERRLTHPAIVAISQVARNEVFGDAARSTGADGGAQHREPTVAGRNGHAAGPTRPR
                     RKPLP"
     gene            1961..2362
                     /locus_tag="PLL14_02835"
     CDS             1961..2362
                     /locus_tag="PLL14_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOG02886.1"
                     /translation="MHIIIQAAGFDLSAGLREHVERRIHFALDRASQYVRKVSIRLSD
                     VNGPRGGEDKRCRIQVTVAGAPDMLIEDTEPDLYVAIDRAADRSGRTLARLLARLREH
                     RHESPRGTRSRGVAIAGKVENGGAALNGGAA"
     gene            2359..3240
                     /gene="htpX"
                     /locus_tag="PLL14_02840"
     CDS             2359..3240
                     /gene="htpX"
                     /locus_tag="PLL14_02840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOG02887.1"
                     /translation="MKRTLLLIGTNLAIMLVLGLVTTLTGAHRFFSGAGLDLGKLLFF
                     ALIMGFGGAFISLWMSKTLAKWSTGAQVIESPGNSTEYWLFETVRRFSEQAGLPMPEV
                     AIYEGEPNAFATGATRNRSLVAVSTGLLQNMTRAEAEAVIAHEVAHIANGDMVTLTLI
                     QGVVNTFVVFIARVVGWLVDSALRKGDAESSGPGIGYTITVVVCDIVFGILASIIVMY
                     FSRSREFRADAGAAKLLGTPRPMVDALRRLGGMEAGELPQNVATAGINGRPGWMALFS
                     SHPPIEERIAALEGGRR"
     gene            complement(3369..4268)
                     /locus_tag="PLL14_02845"
     CDS             complement(3369..4268)
                     /locus_tag="PLL14_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOG02888.1"
                     /translation="MWFKNLHLYRLPRNWATDTARLEEQLAKLTLQGCHATDPKSIGW
                     VPPRAGGTLIHSVNQQWLLALGAEEKLLPASIVKRFASDKAKEIEEAEGRRIGRREMR
                     EIQEAMTLELLPRAFIRSRSTFGWIDPINGWLVIDSASPAKAEEFLEQLQKSVDGLPA
                     RVLKVIQSPSAAMTGWVASGEAPAGFTLDQDLELRSTEKATVRYVNHTLDGEEIRQHI
                     ATGKIVTRLAMTWNDRISFVLNDLLQIKRLAFLDILKEQADGQGENEDERFDIDFTLM
                     TGEVAQLLDDLLVALGGEPVAAN"
     gene            complement(4431..5519)
                     /locus_tag="PLL14_02850"
     CDS             complement(4431..5519)
                     /locus_tag="PLL14_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HOG02889.1"
                     /translation="MTRRRFASVPVWSIAVPVLACILLAAIWGLAPGWFLSAIAGSAL
                     IASVLVAVHHAEVIAHRLGEPFGTLVLALAVTVIEVSLIVSMMRAGGDVSSALARDTV
                     YATVMIVCNGVIGLCLLMGSIRHRTIGFRVEGTTPALAVLATLTTLTLVLPTYTSSTA
                     GPTFSAPQLVFAGVVSFVLWGVFVFVQTVRHRDYFLPANGQSEDAHAAPPSGATALIS
                     LVLLLVSLVAVVGLAKVLAPSIEAAVVGAGMPHAVVGIAIALMVLLPETMSAVRAARS
                     DRMQTSFNLALGSALATIGLTIPAVAATSILLGLPLDLGLPPKEVALLALTLLLTSTT
                     LSGGRATILQGAVHLVVFAVFVFLAMVP"
BASE COUNT         1077 a         1830 c         2016 g         1036 t
ORIGIN      
        1 ggtggtcgtt ggctgcctgc aggcggatga agctgagctt ggcgttgcgc acgaaaccgg
       61 cggcgtcaaa gtcctccggg atatggctgt cggtgctgga gccggttgcc gcggcacccg
      121 aggccggcag cgcaagttca tgtgcgccgc ggtttggtgg gaggccatcg gcgccgaccg
      181 ccgcatactg caggccaccc gtttcgtccg tcgctggccg ctgggtcccg gcgcggcggc
      241 gcatgacgaa gccgatgacc agtacgaccg ccatcaccag caacccgatc atgagcatcg
      301 aggcgagttc ttcgccgaaa ccgaagtgcg aggcgagggc ggcgagtccg aggccggcgg
      361 cgaggccggc gagcggcccc atccacgaac gcttgggttg ggctgacggt gcggccgcag
      421 gcgacggggc tttcgcggca ctggtcgcgg aagccggcgc aggtgtcgtg gtcagcgatc
      481 tttccgcggt ggtttcgcgt tgcatgccgc tcgacttgcc ggcaccaaag cgcttgctgg
      541 ctgcctcggc gtcgccgatt gccagcgtga gggcgaacgc cagaatggcg gtgagtagtg
      601 agaaggtctt catcgtggtc tcctgtgggt cagtggtcgt acagcgacaa ccataaacgc
      661 tctgtcaccg ttataaaatc agaattatcg agaccaatgg ttcgtggaaa actgatgttt
      721 taccgtccgc ggccattctc cgtcaggcaa gcgtcgggcc aacatgccgg cagcgcagcg
      781 tatccggggg atttcctcgg tcttgtcgcg cgggagcggc ccggttgggt tacgggaggg
      841 gcttcctgcg cggcctggtt gggccggcgg cgtgcccgtt gcgacccgcg actgtcggtt
      901 cacggtgctg cgcgccgccg tcggcgcctg tgctgcgcgc cgcgtcgccg aagacctcgt
      961 tgcgcgccac ctggctgatg gcgacgatcg ccgggtgtgt cagccgacgc tcggtggtga
     1021 tcgcgaagag ctgttcggcg atcgaatcaa tgcgccccag ggaagtcacg cgatactgtt
     1081 cgcaaacgta ggcggcgatt gccgtcgggg cgacgaaaag gccgtcgccg gcctgtccga
     1141 aggccttgag cagcgcgctg tcgtcgaact ccccgatgat ccgcggatgc aggcgctggg
     1201 cttcgaacca gtgttcgagg cggggccgaa tggcgacgtc ttcgcccggc atcagaaagg
     1261 gagcgccgtc gagcatggcc gggaagctgt cgttcggtcg tagcgtctcg accagcgcgg
     1321 cggcgccgaa aaccgtcagc gtgctctcgc caagcagatg gttgtagccg cgcacgttga
     1381 gattcgcggg catcgggcgg tcggcgatca ccatgtccag ccgatggatg gccagttccg
     1441 ccagcagggc ggtcagacgg ccctcgcgac agatcagccg taccggctcc ggcaggtgca
     1501 acgcgggttc caccagccgg tgggccaccg acttgggcac cgagtcggct atgccgaccg
     1561 caaacggcag gctcttcgtt gccgtctggt cgcgcacggc gtcgagcagt tcgtcgccga
     1621 gcgcaaagat ctcttcggcg tagccgagga tgcggcggcc gctgtccgtg agttccaggc
     1681 cacgcccgac gcggcggaac agggccaccc cgagggcttc ctcgagttcg cgaatctggc
     1741 cgctgatcga ctgtggcgtg agatgcaact gttcgccggc gcgtgcgatg gcgccggatt
     1801 tggcgaccac ccagaagtag cgcaggtgtt tgaagttgag tgcggccatg tcttgaaaaa
     1861 catccagatt atcgatctat tgatcaatta tattcgattt gttggattgg ttgccgtgcg
     1921 ctacagtttt gcccgttgcc aacccacaag gagttcctcg atgcacatca tcatccaggc
     1981 cgcaggtttc gatctctccg ccggtttgcg cgagcacgtc gagaggcgca tccatttcgc
     2041 gcttgatcgg gccagtcagt acgtgcgcaa ggtttcgatc cgtctttccg atgtcaatgg
     2101 tccacgcggc ggggaagaca aacgctgccg tatccaggtg acggttgccg gggcccccga
     2161 catgctgatc gaggataccg agcccgacct ttatgttgcc atcgaccggg cggccgaccg
     2221 ttcgggacgg acactggcgc gactgttggc acgactgcgc gaacatcgcc acgaaagccc
     2281 gcgtggaacc cggtccaggg gcgtggcgat cgccgggaaa gtcgaaaatg gcggagcggc
     2341 cctaaacggg ggcgcggcat gaaaaggaca cttctgctca tcggcaccaa cctggcaatc
     2401 atgctggtgc tgggtcttgt caccaccttg accggcgccc acaggttctt ctccggcgcc
     2461 gggcttgacc tcggcaagct gctgttcttt gcattgatca tgggtttcgg tggcgccttc
     2521 atctcgctat ggatgtcgaa gaccctcgcc aagtggagca ccggagcgca ggtgatcgaa
     2581 tcgccgggca actccacgga gtactggctg ttcgagacgg tgcgccgatt ctccgagcag
     2641 gcgggtctgc cgatgcccga ggtggcgatc tacgagggcg agccgaatgc cttcgcgacg
     2701 ggcgccaccc gcaatcgttc gctggtcgcc gtctctaccg gcctgctgca gaacatgacg
     2761 cgcgccgagg cggaggcggt gatcgcccac gaggtggcgc acatcgccaa tggcgacatg
     2821 gtgaccctga cgctgatcca gggggtggtg aacacctttg tcgtgttcat cgcccgcgtc
     2881 gtcggctggc tggtggattc ggcgctgcgc aagggagatg ccgagtcgtc ggggccgggc
     2941 atcggctaca cgatcacggt ggtggtgtgc gacatcgttt tcggcatcct ggccagcatc
     3001 atcgtcatgt acttctcgcg cagccgcgag tttcgcgccg atgccggagc cgcgaagctc
     3061 ctgggcacgc cgcggccgat ggtagacgcg ctacgtcgcc tcggcggcat ggaagccggc
     3121 gaactgccgc agaatgtggc gaccgccggc atcaatggcc ggccgggctg gatggccctg
     3181 ttttccagcc atccgccgat cgaagagcgc atcgcggcgc tggaaggcgg ccgtcgctaa
     3241 aaccggctga ttgcccggca cgcgggttgg tcaaactcgg gatgccggat cgttcgatac
     3301 gctgaccgcc tgtccgccag ggacgcggtc agctgttccg ccccgacgcg gcggcggtca
     3361 gtctcagcct aattcgcggc caccggctcg ccaccgagtg cgaccagcag atcgtcgagc
     3421 aactgcgcca cttccccggt catcagtgtg aagtcgatat cgaagcgctc gtcttcgttc
     3481 tccccctggc cgtccgcctg ctccttgagg atgtccagga aggcgaggcg cttgatctgc
     3541 aggagatcgt tgaggacgaa ggaaatgcgg tcgttccagg tcattgccag gcgagtgacg
     3601 atcttgccgg tggcgatgtg ctggcggatc tcctcgccat cgagcgtgtg attgacgtag
     3661 cgcacggtcg ctttttccgt ggaccgcagt tccaggtcct ggtcgagagt gaagccggcc
     3721 ggcgcttcgc cgctggccac ccagccggtc attgccgccg agggcgattg tatgaccttg
     3781 agcaccctgg ccggcaaacc gtcgaccgac ttctgcaatt gttcgagaaa ttcctcggct
     3841 tttgccggcg aggcggaatc gatcaccagc cagccgttga tcgggtcaat ccagccgaag
     3901 gtgctgcggc tgcggatgaa ggcgcgcggc agcagttcga gggtcatcgc ctcctggatc
     3961 tcgcgcatct cgcggcgccc gatgcggcgg ccttcggctt cttcgatctc cttcgccttg
     4021 tcactggcga aacgcttgac gatcgacgcc ggaaggagct tttcctcggc accgagggcg
     4081 agcagccact gctggttgac gctgtgaatc agcgtaccgc cggcacgtgg cggcacccag
     4141 ccgatactct tcggatcggt ggcatgacat ccttgcaggg tcagtttggc gagttgctct
     4201 tcaagccggg cggtgtcggt cgcccagttc ctgggcaggc ggtagagatg aagattcttg
     4261 aaccacatgg tcggacccga aaaagtcgag gattataggc cggaaagggg gcgaccatcg
     4321 ggttgatccc gaacctgccg tcggcaactt ggccgcaggc tgcgccgata ttcgccgctg
     4381 cggggctggc ctggctgaac gttcacaccg ggccggcacg ggttgcggta tcacgggacc
     4441 atggccagaa agacaaatac ggcgaacacc accagatgaa cggcaccctg aagaatcgtc
     4501 gcgcgtcctc cggacagtgt cgtcgaggtc agcaacagcg tcagtgccag cagggccact
     4561 tccttgggag gcagtcccag gtccagcggt agaccgagca ggatggaagt cgcggccacg
     4621 gcgggaatcg tcagaccgat ggtggcgagc gcagagccca gcgccaggtt gaagctggtc
     4681 tgcatccggt cgctgcgcgc cgcgcgcacg gctgacatcg tctcgggcag cagcaccatc
     4741 agcgcgatgg cgatacccac cacagcatgc ggcatgcccg cgccaacgac ggcggcctcg
     4801 atgctggggg cgagcacctt ggcgaggccg accaccgcaa caagagaaac gagcagcagc
     4861 accaggctga tcaatgcggt tgctcccgag ggaggggccg cgtgcgcgtc ttcggattgg
     4921 ccgttcgcgg gcaggaaata gtcgcgatgc cgaaccgtct ggacgaaaac gaacacgccc
     4981 cagagcacga aggaaacgac gccggcgaag acgagttgcg gtgccgaaaa agtcggaccc
     5041 gccgtggacg aggtgtaggt gggaagaacc agggtaaggg tggtgagggt ggccagtacc
     5101 gccagagcgg gggtggtgcc ctcgacgcga aagccgatgg tccgatgccg gatactgccc
     5161 atcagcagac aaagaccgat gacgccgttg cagacgatca tcaccgtggc gtacacggtg
     5221 tcgcgtgcca gagcgctgga aacgtcgccg ccggcccgca tcatcgagac gatcaacgac
     5281 acttcgatga cggttaccgc caaagcgaga acgagcgttc cgaacggttc gccaagtcgg
     5341 tgcgcaatca cttcggcgtg atgaacagcg accagcaccg acgcgatcaa ggcggaaccc
     5401 gcaatggcgg acaggaacca tccgggagcg agcccccaga tcgcggcgag caggatgcag
     5461 gccagaaccg gtacagcaat actccagaca ggcacactgg caaaccgtcg acgggtcatg
     5521 ggaggcagcc tcaggttgat gaagagcgcg cttcagcgcg gtggctcgca gcgaagttta
     5581 atgtgaaagt cgcgccagcg actcacgaac cttccgtcgc ccgcgtgcgg ggaagcaacc
     5641 gcaggacggt gcggaagatc gatcgacgag tgcaggagcg caaacagtcg cagacaggaa
     5701 accggcttcg acgcctcgca cattccgccg tcactgcgcc agcgccttgc gaaagacgta
     5761 aatgagagta gaaccgattt cctccggatt tcaatgatga cgcgatgccg gccaggccag
     5821 ctgcggcatc cgccggcatc gatgcctgat ccggtcgccg cgcggacatc aggacgcacg
     5881 gttgtagcgg actcccccgc acgcccgtac cggcaagcag gcgtcctttg actgtgggac
     5941 aattcgcgcc tcaccattc
//
LOCUS       DAOSIT010000070         5942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12076_length_5942_cov_8.688806, whole genome shotgun sequence.
ACCESSION   DAOSIT010000070 DAOSIT010000000
VERSION     DAOSIT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12076_length_5942_cov_8.688806"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLL14_02855"
     CDS             <1..1414
                     /locus_tag="PLL14_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012272698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG02890.1"
                     /translation="RDEKAMYADQEPISGPITFQEAAARALKYNLDYRLKLMENSLSR
                     NLYDVSKYEMLPRLVAGAGYLARNNDSGGTSVSIETGEQTLSPSTSQQRHQTLANLSL
                     SWNLLDFGVSYYRAQQKADQVMMADERRRKVVQNVLQDVRNAYWRALGAQRLLSQVDS
                     LLDRVRLALQMARKVEKEGLMPRSQVLAYQRALLDAVTLLSLRRQDLELSKAELVALM
                     SIPPGVSIVLADDTRIESLPSPPANIAALEALALRQRPEIMEEWYRKRITENDIKAAK
                     LLVWPNVSFDISGQYDSNIYLYNQSWIDTGLRVSWNIMKLAQLPALERAQESQNQTDD
                     MRRMALSMAVLTQVRVGVQRYALSRMEFGVAEESLRVDEELLKYARAAARTSFDSELE
                     VIRAEARALLSRYQQHAAYSNAQAAWGRVYNSVGLDVMPEAIESHDIKTLAAAIDTTL
                     RQWQENLLTGTAFDGRDKVE"
     gene            1407..2198
                     /locus_tag="PLL14_02860"
     CDS             1407..2198
                     /locus_tag="PLL14_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG02891.1"
                     /translation="MNRALVLFLGLWAAGFAQAQNAPAAASPASPAKAAAVRTASTDP
                     DAIRIVLAPELETTLVAQMPGRIEAIAATLGSRVSKGRVVLRMDCGESDARRKMADAE
                     LAAAIETLSTKRKLREMDAAGDMEIKLAVAAADKARGAVALARSQTAYCSVIAPFNGR
                     VARLYVKNFQGVNVGTPLIDLVSDGPLKLRLNIPSTSLRQVREGSPFEVIVLETGKTY
                     PARVTAINSRVDAVSQTVEIEGRLQGTPAELLPGMSGTARFPPAP"
     gene            2222..3562
                     /locus_tag="PLL14_02865"
     CDS             2222..3562
                     /locus_tag="PLL14_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG02892.1"
                     /translation="MTDEVALYSAIATLEARARAVGSVAELMVILANDVHPLLPFQQA
                     LVFAQPHQTWELAAVSGLARPSEDSPYLVWLRRMATWLAAQIAVGESGPRWLTAADLP
                     VGRDDLRDGWHEWWPSGLWVASLYRQSGAALGHLCFLLDAPPAPVLLPHLERLFATYS
                     HEWDLATRQDHRWRVNFTARQKRFACLAVAMLCLLPVRQSALGPAEIISLDAIAVASP
                     LDGVVKTFHVRPNQAVAAGEPLFSLDDTTLKSRLEVAQRSVQVADAELLSATHKAFDN
                     TQSKSEITLLTGRAQEKRAELAATKHQLARIDVLATRAGVAIFGDVNDWIGKPVVTGE
                     RIMQLANPAHLGLLVYLPVADAIALEEGAPVKLYLTTQPLSSLDGKIFQTSYQATFSD
                     EGIASYRLQGRFTSPPGDVRIGLRGTAKVSGGWVVLGYYLIRRPLAALRESLGV"
     gene            3581..5677
                     /locus_tag="PLL14_02870"
     CDS             3581..5677
                     /locus_tag="PLL14_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005901210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HOG02893.1"
                     /translation="MSGTTASPGALREDLHLHEASSDPDGSPAWVIEDPVVNRFYRIG
                     WLEFECLLRWGDPAPEIAAAISRDTPLDPDASQVEDFQRFLAQHSLLRPDDEMLAQLL
                     SQARQPGSWKRWQWWLHHYLFFRIPLLRPQYALQRLAGVLAPLFRPWAVWATLASIVT
                     GLLLVARQWEVFVSTLVESVSPAGLLGFLLALSCAKALHELAHALVATHYGVRVAHMG
                     IAFLVLWPMLYTDTGESWKLKMRRQRLAVAAAGMTTELALAGLATLVWSIADPGPTRS
                     AAFYLATTGWTLSLALNASPFMRFDGYFILSDLLDLPNLHERSGALARAALRRTLFGW
                     SEPDREYFPPWPRRALIGFAFATWIYRLGVFLAIAAVVYYFFFKVLGIFLFLVEITWF
                     IARPIWSELRVWCRRWQETPAQRRRTWHFALFLLVILAFIPWRSDIHGPGLVHPLLQQ
                     RVFAPFPAQIGVVRSPGAVLAGEELVRLYSQDLHDQERQTQAGIIALGERLSGIEAVE
                     GALDQRNATRERLGEQLARGQSVNDELIRLSIRAEFNGHWLDVDPLAGSGSWLGTLKP
                     IGVLIDPAVWVVDAYVGQADIERIEVGAAARFFPEHQNVALTGRIVEVDLVRSQRLPH
                     AMLTIRHGGPIATVTGGNELQPIDSRYRVRVRLDEPPQNWRELRGSVSLTGKSESLAY
                     AFVVKALSVLIRESGF"
     gene            complement(5779..>5942)
                     /locus_tag="PLL14_02875"
     CDS             complement(5779..>5942)
                     /locus_tag="PLL14_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HOG02894.1"
                     /translation="VGSGDTSRIWLLVNEDERLDQMVKQVEKLEDVLAVRRHGAQHEV
                     FVRLEEFFL"
BASE COUNT         1043 a         2025 c         1789 g         1085 t
ORIGIN      
        1 acgcgacgag aaggccatgt acgccgatca ggaaccgatc agcggcccga tcacttttca
       61 ggaagctgcc gcgcgcgcgt tgaagtacaa cctcgattac cgcctgaagc tgatggaaaa
      121 ctcattgagc aggaatctct acgatgtctc gaagtacgag atgctgcccc gtctggtcgc
      181 cggcgcgggc tatctcgcgc gcaacaacga ttccggtggc acctcggtga gcatcgagac
      241 cggtgagcaa acgctgagtc catccacctc gcagcaacgt caccagacgc tggccaatct
      301 gtcgctgtcg tggaatctgc tcgacttcgg cgtttcctat taccgtgccc agcaaaaagc
      361 cgaccaggtg atgatggccg atgaacgccg tcgcaaggtc gtgcagaatg tcctccagga
      421 tgtccgcaac gcgtattggc gggcgctcgg cgcgcagcgc ctgttgtcgc aggtggacag
      481 tcttctcgac cgcgtgcgcc tggccctgca aatggcgcgc aaggtcgaga aggaaggcct
      541 gatgccacgt tcgcaggtgc tcgcctacca gcgcgcgctg ctcgacgcgg tgacccttct
      601 atcgctacgc cgacaggacc tggaattgtc gaaagccgaa ctcgtcgcgc tgatgtcgat
      661 tcctccgggc gtcagcatcg tgcttgccga cgatacccgg atcgagtcgc taccgagtcc
      721 cccggccaac atcgctgcgc tcgaagcgct ggcgctacgc cagcgccccg aaatcatgga
      781 agagtggtat cgcaagcgga tcaccgaaaa cgacatcaag gccgccaagc tgctggtctg
      841 gccaaacgtc tccttcgaca tcagtggcca gtacgattcc aacatctatc tgtacaacca
      901 gtcgtggatc gacaccggcc tgcgtgtctc ttggaacatc atgaagctcg cccagttgcc
      961 ggctctcgaa cgtgcgcagg agagccagaa ccagaccgac gacatgcgcc gcatggcgct
     1021 gtccatggcc gtgctgacgc aggtgcgcgt cggcgtgcaa cgctatgcgc tgtcgcgcat
     1081 ggagtttggc gttgccgagg aaagcctgcg cgtcgacgag gaactcctca agtacgcgcg
     1141 tgccgccgcg cggacgagct tcgactccga actcgaagtc atccgtgccg aggcgcgcgc
     1201 gctgctctcg cgctaccagc agcacgccgc ctattcgaat gcccaggccg cctgggggcg
     1261 ggtctacaat tcggtcgggc tggatgtcat gccagaagcg atcgaaagcc acgacatcaa
     1321 gacgctggca gcggcgatcg acacaaccct tcgtcaatgg caggaaaacc tcctcaccgg
     1381 cacggctttc gatggcagag acaaagttga atagggcgct cgttctcttc ctcggcctgt
     1441 gggcggcagg cttcgcgcag gcacagaacg cgccggcggc agcatcaccc gcctcgccag
     1501 cgaaagccgc cgccgtccgc acggcgagca ccgaccccga cgcaatccgc atcgtgctgg
     1561 cacccgaact cgaaacgacg ctggtcgcgc agatgccagg acgaatcgaa gccatcgccg
     1621 cgacgctcgg ctcgcgcgtg agcaagggac gcgtcgtcct gcgcatggac tgcggcgaaa
     1681 gcgatgcccg ccgcaaaatg gccgacgccg aactcgcggc ggccatcgaa acgctgagca
     1741 ccaaacgcaa actgcgggaa atggacgctg ccggcgacat ggaaatcaaa ctcgcggtcg
     1801 ccgcagccga caaggcgcgg ggcgcggtgg ccctggcacg atcgcagacc gcctactgca
     1861 gcgtcatcgc gcctttcaat ggccgcgtgg cgcgccttta cgtcaagaat tttcaaggcg
     1921 tcaacgttgg tacgccgctg atcgacctgg tcagcgacgg cccgctcaag ctgcgcctga
     1981 acattccatc gacttccctg cgccaggtca gggaaggcag tccgttcgaa gtcatcgtcc
     2041 tcgaaaccgg caagacctac ccggcgcgcg tcaccgcgat caattcgcga gtcgacgctg
     2101 tttcgcaaac cgtcgagatc gaaggccgac tgcagggaac gcctgccgaa ctgctgccag
     2161 gaatgagcgg caccgcgcgt ttcccgccgg ctccctgata gcgcacagag ctacccggac
     2221 gatgaccgac gaagttgcgc tgtacagcgc gattgccacc ctcgaggcgc gagcgcgcgc
     2281 ggtcggcagc gttgccgaac tgatggtgat cctcgccaac gatgtccatc cgctgttgcc
     2341 tttccagcag gcgctggtct tcgcccagcc acatcagacg tgggaactgg ccgcagtttc
     2401 cgggctcgcg cgaccgagcg aggactcgcc ctacctcgtc tggctcaggc gaatggcgac
     2461 ctggctcgcc gcgcagatcg ccgttgggga aagtggcccg cgctggctga ctgccgcaga
     2521 cctgccggtc gggcgtgacg atctgcgtga tggctggcac gagtggtggc cgtccggtct
     2581 atgggtcgct tctctctacc ggcaatccgg tgcagcgctc ggccaccttt gttttctgct
     2641 cgatgcgcca cctgcgccgg tgcttcttcc tcatctcgaa cgtctcttcg ccacttattc
     2701 tcacgagtgg gacttggcaa cacgccagga ccaccgctgg cgagtcaatt tcaccgcccg
     2761 tcagaagcgc ttcgcctgcc ttgccgtcgc tatgctttgc ctgctgccgg tgcgccagag
     2821 tgcgctcggt ccggcggaga tcatttcgct cgatgcgatt gccgtcgctt cgccgctcga
     2881 cggtgtcgta aagactttcc atgtccgtcc caaccaggcg gtcgccgctg gcgagccact
     2941 cttctcgctc gacgacacga ccctgaaaag ccgtctcgaa gtcgcccagc gctcggttca
     3001 ggtcgccgac gccgagttgc tctctgcgac tcacaaggct ttcgacaata cccagagcaa
     3061 gagtgaaatc actttgctca ccggccgggc gcaggaaaag cgtgccgaac tcgccgccac
     3121 gaagcaccaa ctggcgcgca tcgacgtcct ggccacacgt gccggcgtcg ccattttcgg
     3181 cgacgtcaac gactggatcg gcaagccggt ggtcaccggt gaacgtatca tgcaactggc
     3241 caacccggcc catctcgggc tgctggtgta cctgccggtg gccgacgcga ttgcccttga
     3301 agaaggtgcg ccggtcaagc tctatctgac gacgcaaccg ctgtcgtcac tcgacgggaa
     3361 gatcttccag acgagttatc aggcgacatt ctccgacgag ggaatcgcca gctaccgcct
     3421 gcagggacgc ttcaccagcc cgccaggcga cgttcgcatc gggctgcgcg gaacggccaa
     3481 agtctccggc ggttgggtcg ttctcggcta ttacctgatc cgccgcccgc tcgccgcgct
     3541 gcgcgagagc ctcggcgtct gaatgccgac ggcgacaacc atgagtggaa cgactgcatc
     3601 tcccggcgcg ctacgcgaag acctgcacct gcacgaagcg agcagcgacc ctgacggatc
     3661 gccggcctgg gtgatcgagg atccggtggt gaaccgcttc taccgcatcg gctggctcga
     3721 attcgagtgc ctcttgcgat ggggggatcc ggcaccggaa atcgccgccg cgatctcccg
     3781 agacacgccg ctcgatcctg atgccagtca ggtcgaagat ttccagcgct tccttgcgca
     3841 gcattcgctg cttcgaccgg atgatgaaat gcttgcccag ttgctgagcc aggctcgcca
     3901 gcccggatca tggaaacgct ggcagtggtg gctgcatcac tacctcttct tccgcatccc
     3961 gctgctccgc ccgcaatatg ccctgcagcg cctggccggc gtactcgcgc cgttgttccg
     4021 gccgtgggcg gtctgggcta cgctggcctc gatcgtcacc gggctgttgc tcgtcgcgcg
     4081 ccaatgggaa gtcttcgtca gtacgctcgt cgagtcggtt tcgcctgccg ggctgctcgg
     4141 cttcctcctc gccctgagct gcgccaaggc gctgcacgaa ctcgcgcatg cgctcgtcgc
     4201 cacccactac ggcgtccggg tggcgcacat gggaatcgcc tttctcgtcc tctggccaat
     4261 gctgtacacc gataccggcg aatcgtggaa actgaagatg cgccgccagc ggctcgccgt
     4321 cgccgccgcc gggatgacca ccgaactggc tctcgccggg ctggcaacgc tggtctggag
     4381 catcgccgac cccggcccga caaggagcgc cgctttctat cttgcgacca ccggctggac
     4441 gctttcgctg gcactcaatg ccagcccctt catgcgcttc gatgggtatt tcattctctc
     4501 cgacctgctc gacctgccca atctgcacga acgttccggc gcgctcgccc gcgccgcgct
     4561 gcggcgaacg ctctttggct ggagcgagcc cgaccgcgaa tacttcccgc cgtggccgcg
     4621 ccgcgcgctg atcggctttg ccttcgccac ctggatctat cggctggggg tctttctcgc
     4681 catcgcggcg gtcgtttatt acttcttctt caaggtgctc ggcatcttcc tgttcctggt
     4741 cgaaatcacc tggttcatcg cccgaccgat ctggtccgag ctgcgcgtgt ggtgcaggcg
     4801 ctggcaggaa acaccggcgc aacggcgccg cacctggcac tttgccttgt tcctgctggt
     4861 catcctggct ttcatccctt ggcgcagcga catccatggc ccggggctcg ttcacccttt
     4921 gctgcagcaa cgcgtcttcg caccgtttcc agcccagatc ggggtcgtcc gtagtcccgg
     4981 tgcagttcta gccggcgagg aactcgttcg cctctacagc caggaccttc acgatcagga
     5041 gcgccagacg caggccggaa tcatcgcgct tggcgagcgg ctttccggaa tcgaggcggt
     5101 cgagggtgca ctcgaccagc gcaacgccac gcgcgaacgc ctcggcgaac aactcgcccg
     5161 cggtcagtcg gtcaatgacg aactcatccg cctgagcatc cgcgccgagt tcaacgggca
     5221 ctggctggat gtcgacccgc tggcgggcag cggcagttgg ctggggacgt tgaagccgat
     5281 cggcgtcctg atcgatccgg cggtctgggt cgtcgacgct tacgtcggcc aggccgacat
     5341 cgaacggatc gaagtcggcg ccgctgcccg cttttttccg gagcaccaga acgtcgcact
     5401 cacaggacgg atcgtcgaag tcgacctggt gcgtagccag cgcctgccgc acgccatgct
     5461 cacgatccgt catggcgggc cgatcgccac ggtcaccggc ggcaacgaac tccagccgat
     5521 cgattcgcgc taccgagtgc gcgttcgcct cgatgagccg ccgcagaact ggcgtgaact
     5581 ccgcggcagc gtttccctga caggaaaaag tgaaagcctg gcctacgcat tcgtcgtcaa
     5641 ggcgctctcc gtgctgatcc gggaaagcgg cttttgatcg ggcttcaggc gcctcgcccg
     5701 tcgtatgtag ctgccgcttc gggtgccgac cagccgcagc ggtcgcactt tcccacccag
     5761 cagccgctgg ccatccggtt aaagaaagaa ttcctccagc cgcacgaaga cttcatgctg
     5821 ggcaccatgg cggcgcaccg ccaggacatc ctcgagcttt tccacctgct tcaccatctg
     5881 atccaggcgc tcgtcctcat tgaccagcag ccagatgcga ctggtgtcgc cgctccccac
     5941 cg
//
LOCUS       DAOSIT010000071         5939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12085_length_5939_cov_9.608600, whole genome shotgun sequence.
ACCESSION   DAOSIT010000071 DAOSIT010000000
VERSION     DAOSIT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_5939_cov_9.608600"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5939)
                     /locus_tag="PLL14_02880"
     CDS             complement(<1..>5939)
                     /locus_tag="PLL14_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOG02895.1"
                     /translation="AWGWTYDNQAITLELGEAHPNAPWHAADLDGDVRMDLARVWQNA
                     DGRWVLESWLTKDEADAETTTMRLEQRPDLVLDLGPGETDFRFLDHNGDGRADLVGTQ
                     TNQQGEEAITLWLNDGPGFVALPASHRDRGARVLPADLNGDGRGDLLELSRTTEGTAV
                     ATPWLSTGDGFTARSSVDLGIWQASTHFLVGDLDADGRSDLLEIRRQDDGQVSANTWL
                     AGTEHWLPGGSTALGTLPQSSGYTLSDSTGDGRADLMVYSLPGNGNTRFTIWPGNGRT
                     FAEAAYQETPSENLTTSAVLPLDLNGDGRGDFAYVDREPDANSGGYTTAMRWWQGSAD
                     GRWEHYDGDTTRELQPFAQHFTYLTGDVDGDARQDVLRVWSDAHVHLDIRRSHGSNLG
                     DWTSPSTLGNTRNRQQFLSADVNGDGRADLLQIWRDNDGEAVASVWMATVVGEQVQYL
                     QGGDSVLGAWYEDSIWLAQDHNGDGRSDLVGSWQESDGRRQIGTWLSDGVSLTTLHQP
                     RSDVVTFDADFNGDGRRDLLEVSRRADGMAVATPWLSTGSGFAAGRGAELGVWLADTP
                     YRIGDLDGDGSSDLLEIRLQPNGNVVANTWLGGATTLVAAATTNLGPLAANSHYTLGD
                     TTADGRADLLVQSPSSDGASRFTLWQGDGRRFREQSSQEEARATTPDAQILPLDMNGD
                     GSGDLVEVQRDYRHDANLSDQSTRLRWQNVSPDGIWSSGEQSIGVWSRNVSMLATDAD
                     GDARGDVLRISKADDGQMQLTLWRSQGNAFDNWGDSTLGAARLNQEFHAADLNGDGRG
                     DLLQIWQDSNGRAVATRWLATLVDHRISYLAAGDNNLGIWHAGATYVVQDHNGDGRAD
                     LVASWQESDGTRHIGAWLTDGVSLPGHPDRGQELRMLAADFAGDDRSELLELSRRPDG
                     MAMARLWLPTGDDLTGGFTPGNSSLLGSWQASTQYLVGDLDADGRSELLEIRRQADGE
                     VVANSWRPGADGLLAVGSTALGQYPPNSRYTLTDTTGDGQADLVVQWLPGNGVNRLTV
                     WQGSGLGFSRSGDRNPYRDETTSATALSLDYNGDGRGDFVFVPRDADQDPNTSELSTR
                     LLWLEGAPTGLLSTRYHDGGLGIWSQNVDFLTGDANGDGLDDVLRIWKNEDDTLRVTA
                     WRSAGSSHDDWRFEESTTDILAGALANQQFVAADVDGDGRTDLLQIGQDDSDRAIARV
                     WLAGESGYAAAGGGVLGAWLAEAHYFSLPGLSGAADLVRSAADGEGAARFDRWRYVGG
                     IASGDSLLPIVTQPLPPAGSDNFDWGPFDQSETTTPQADLTIGSVRIAPAGNWSPGDL
                     VTVSWSTTNSGTLDIDNTWSERLEVYNGSTGELIATRTVVQELVDGRPALASGESSKR
                     LLSFVWPDDTTGSGRIIFRIVVDDHNSVPENNAGGTGEANNESSYSVANAPDLRVINL
                     HTLPGERFAGDELHISWEDRNDGSSATQTGWNDHLLIRNRSTGEILFDAAVPYDPAQP
                     GNGSIAAGASRLRSQVFRLPEGVRGVGEIEISVSTDEGRDGQGALLEINTRGDAESNN
                     RAVTTTTATARPYADLRVDALVAPSSAVGTLPIRVSWSVSNQGSTDADQDWTDQIVYS
                     TDALIGDADDVVIASVRHSGGLRVGESYSETAEVTIPIRSAGRYHLGIRSDSGTEVLE
                     PDTRADNQTARAIDIIAPYADLVLDEVNVPRGARSGETIVVNWVVSNQGNVTTNRSSW
                     SDRVVLSSDTTLSSDDLVLAGAVAHAGVLAAGQSTPGRATITLPRDLSGDYFVIVQTN
                     AGRNVDEAGLTLNNTRASNDVLAISLTPTPDLTVTEVLGPSALRPGDTASLRYTVSNS
                     GTAATSNAWRDRIYIDRGTHGGLQQVATVLHTSSLQAGGTVTQTVDFVLPGASPEGEF
                     RWLVRTDADNSLYERHAEDNNETTASVQVARPDLVVSEVRAPTLARSGERIEVAWRVG
                     NQGASASGDWVDTVYLVQGTLT"
BASE COUNT          894 a         1956 c         1969 g         1120 t
ORIGIN      
        1 gtcagcgtgc cctggacgag gtacaccgta tcgacccagt ctccggatgc gctggcgccc
       61 tggttcccga cgcgccacgc gacctcgata cgttcacccg aacgggcgag agtcggcgct
      121 cggacctccg acaccaccag atcgggacgt gcgacctgca cgctggcggt tgtctcgttg
      181 ttgtcttcag cgtggcgttc gtagaggctg ttgtcggcat cggtcctgac cagccagcga
      241 aattctcctt ccggagaggc gccgggcaga acgaaatcga ccgtctgcgt gaccgtgccg
      301 ccggcctgta ggctgctggt atgcaggacg gtggcgacct gctgcaggcc tccgtgcgta
      361 ccgcgatcga tgtagatgcg gtcacgccac gcgttggaag tggccgcagt accgctgttg
      421 ctgacggtgt agcgcaggct ggcggtgtcg ccgggccgca gggcgctggg cccgaggacc
      481 tcggtgaccg tcagatccgg ggtcggggtc aggctgatcg ccaggacgtc gttgctggcg
      541 cgggtgttgt tgagggtcag tccggcttcg tcgacgttgc gcccggcgtt ggtctgtaca
      601 atcacgaaat agtcgccgct gaggtctcgc ggcagggtga tcgtcgctcg gccgggggtg
      661 ctctggcccg ccgccaggac gcccgcgtgc gctaccgcac cggccaggac gaggtcgtcg
      721 ctgctcagcg tcgtgtccga agagagaacg acgcgatcgc tccacgacga tcgattggtg
      781 gtgacgttgc cctggttgct gacgacccag ttgacgacga tggtctcgcc gcttcgtgct
      841 ccgcgcggta cgttgacctc gtcgagtacg agatccgcat agggcgcgat gatgtcgatc
      901 gcacgcgcgg tctggttatc ggcccgggta tcgggttcca gcacttcggt gccgctgtcg
      961 ctgcgtatgc cgagatggta gcgtccggcg ctgcgaatgg ggatcgtgac ttcggcagtc
     1021 tcgctgtagc tttcgccgac acgcagccca ccgctgtggc gaacgctggc gatcaccacg
     1081 tcgtccgcgt cgccgatcag cgcatcggtg ctgtagacga tctgatcggt ccagtcctgg
     1141 tcggcgtcgg tggagccctg gttcgacacc gaccaactca cgcggatggg caacgtaccg
     1201 acggcgctgc tcggcgcgac cagggcgtcg acgcgcagat cggcgtaggg ccgggccgtt
     1261 gccgtggtgg tcgtgaccgc gcggttgttg ctttcggcgt cgccacgggt gttgatttcg
     1321 agcagcgcac cctggccgtc ccgaccttcg tcggtactga cgctgatctc gatctcgccg
     1381 acacctcgga cgccctccgg gagcctgaaa acctggctgc gcagccgtga cgcgccggcg
     1441 gcgatgctgc cgttgcccgg ctgcgcgggg tcataaggca cagcggcgtc gaacaggatt
     1501 tcgccggtag agcggttcct gatcagcaga tggtcattcc agccggtctg cgtggcgctg
     1561 ctgccatcgt tgcggtcttc ccaggagatg tgcagctcgt cgccggcaaa gcgctcgccg
     1621 ggaagggtgt gcaggttgat gacccgcagg tcgggagcgt tggcgaccga gtagctgctc
     1681 tcgttgttcg cctcaccggt gccgccggcg ttgttctcgg gcacgctgtt gtggtcatcg
     1741 acgacgatcc ggaagatgat ccggccgctg cctgtcgtgt cgtccggcca gacgaaggag
     1801 agcagacgtt tcgacgactc gccgctcgcc agggcggggc ggccgtcgac gagttcctgg
     1861 acgacggtgc gcgtggcgat gagttcgcca gtgctgccgt tgtagacttc gagccgttcg
     1921 ctccaggtat tgtcgatgtc gagcgtgccc gaattggtcg tgctccagga aaccgtgacc
     1981 agatcgccgg gcgaccagtt gccggctggc gcgatgcgga cgctgcctat cgtcaggtcg
     2041 gcctgtggcg tggtggtctc gctctggtcg aacggtcccc agtcgaaatt gtcgctaccg
     2101 gcgggcggca gcggctgggt gacgatcggc aacagcgaat ctccagaggc gatgccaccg
     2161 acgtagcgcc agcgatcgaa gcgcgccgcc ccctcgccat ccgccgcact gcgcaccagg
     2221 tcggcggcac cgctcagccc cggcagggaa aagtaatgcg cctctgcaag ccaggcaccc
     2281 aggacaccgc cgccagcggc ggcgtagcct gactcgccgg cgagccagac cctggcgatc
     2341 gcccggtctg agtcatcctg tccgatctgc agcagatccg tacggccatc cccatcgacg
     2401 tctgcggcca cgaactgctg gttggccagg gcaccggcga gaatgtcggt cgttgactcc
     2461 tcgaatcgcc agtcgtcatg gctgctgccg gcgctgcgcc aggccgtcac ccgcagggtg
     2521 tcgtcctcgt tcttccagat gcgcagcaca tcgtcaagac catcgccgtt ggcgtcgcca
     2581 gtcagaaagt cgacattctg cgaccagatc ccgaggccgc catcgtggta acgagtgctc
     2641 agcagtcccg tgggcgcacc ttcaagccac agcaggcgcg tgctgagttc gctggtgttc
     2701 ggatcctggt cggcgtcgcg cggcacaaac acgaagtcgc cgcgaccatc gccgttgtag
     2761 tccagcgaca gggcggtggc actggtggtc tcatcgcgat acgggttgcg gtcgccgctt
     2821 cggctaaagc cgaggccgct tccctgccag acggtgagac ggttgacgcc gttgcccggc
     2881 agccattgca ccaccagatc ggcctggccg tcgccggtgg tatcggtcag cgtgtagcga
     2941 ctgttgggtg gatattgacc gagcgccgtg ctgccgacgg ccagcagacc gtcggcgcca
     3001 gggcgccagg agttggcaac cacctcgcca tcggcttgcc gccttatttc gagcagttcg
     3061 ctccttccgt cagcgtcgag gtcgccgacc agatattgcg tgcttgcctg ccacgacccc
     3121 agcaagctgc tgttcccggg ggtgaaaccg ccggtgaggt cgtctccagt cggcaaccac
     3181 agtctggcca tcgccatacc gtcggggcgg cgactcagct ccagtaattc cgaccgatcg
     3241 tccccggcaa aatccgcggc aagcatgcga agttcctgcc cacggtcggg gtggccgggc
     3301 aggctgacgc cgtcggtgag ccaggcgccg atatgccggg ttccatccga ttcctgccag
     3361 ctcgcgacca gatccgcccg accatcgccg ttgtggtcct gtacgacgta ggtggcgcca
     3421 gcgtgccaaa ttccgaggtt gttgtcgccg gcggccaggt agctgatgcg gtgatcgacc
     3481 agcgtagcca gccagcgggt ggccactgct ctgccattac tatcctgcca gatctgcagc
     3541 aggtctccgc ggccgtcgcc gttgaggtcg gcggcgtgga actcctgatt cagccgggct
     3601 gcaccgaggg tggaatcgcc ccagttgtcg aatgcgttac cctggctgcg ccagagcgtg
     3661 agctgcatct gcccgtcgtc ggctttcgag atccgcagca cgtcgccgcg cgcatcgcca
     3721 tcggcatcgg tcgcgagcat gctcacgttc cgcgaccaga ctccgataga ttgctcgcct
     3781 gaactccaga tcccgtcggg cgagacgttc tgccaacgca ggcgggtgct ctggtcgctg
     3841 agattcgcgt cgtggcggta gtcgcgctgc acctcgacga gatcgccact gccgtcgccg
     3901 ttcatgtcca acggcaggat ctgcgcgtcc ggtgtcgttg cgcgcgcttc ttcctgagac
     3961 gactgttcgc ggaaccgccg gccatcgccc tgccacagcg tgaaacggct ggcaccgtcg
     4021 ctcgatggcg attgcacgag caggtcggca cggccgtcgg ccgtcgtgtc gccgagggtg
     4081 tagtggctgt tcgccgccaa tggaccgaga ttggtggttg ccgccgcaac cagggtcgtg
     4141 gctccaccca gccaggtatt ggccacaacg ttgccgttgg gctggagacg tatttccagc
     4201 agatcactgc tgccgtcgcc gtcgaggtcg ccgatgcggt atggcgtgtc ggcgagccac
     4261 acgccgagtt cagcgccacg cccggcagca aaaccacttc cggtggacag ccagggcgtc
     4321 gccacggcca tcccatcggc gcggcggctc acctccagga gatcccggcg gccatcacca
     4381 ttgaaatcgg cgtcgaaagt gaccacgtcg ctgcgcggtt ggtgcagggt cgtcaggctg
     4441 acaccatccg acagccaggt gccgatctgc cggcggccat cgctttcctg ccagcttccc
     4501 accaggtcgg atcgaccatc gccattgtgg tcctgtgcaa gccagatgct gtcctcgtac
     4561 catgcgccga gcacgctgtc gccaccctgc aggtattgca cctgctcgcc gaccacggtc
     4621 gccatccaca cactggcgac cgcttcacca tcgttgtccc gccagatctg caacaggtca
     4681 gcccggccgt cgccgttgac atcggcgctc aggaactgct ggcggttcct ggtattgccg
     4741 agggtgctcg ggctggtcca gtcgccgaga ttgctgccat ggctgcgccg gatgtccagg
     4801 tgcacatggg cgtcgctcca gacgcgcagc acgtcctggc gcgcatcacc gtcgacatcg
     4861 cctgtcaggt acgtgaagtg ctgcgcaaag ggctgcaact cgcgtgtggt gtcgccatcg
     4921 tagtgctccc aacggccatc cgcactccct tgccaccagc gcatggccgt ggtgtagcca
     4981 ccgctattgg cgtcgggttc gcggtccacg taggcgaagt cgccgcgtcc atcgccgttg
     5041 aggtccagcg gtaacaccgc cgaagtggtc aggttttcgg agggcgtctc ctggtacgca
     5101 gcttcagcaa atgtccgacc gttccctggc cagatcgtga accgggtatt gccattgccc
     5161 ggcagcgagt acaccatcag gtcggccctg ccgtcgccgg tactgtcgct cagggtgtag
     5221 ccgctgctct gcgggagcgt gccgagcgcc gtgctgccgc ccggcagcca gtgctcggtg
     5281 ccggcgagcc aggtgttggc tgacacctgg ccatcatcct gacgccggat ttccagcaag
     5341 tcgctgcggc cgtcagcgtc cagatctccc accaggaaat gcgtgctggc ttgccagatg
     5401 ccgaggtcga cgctgctcct tgcggtgaag ccatcgcctg tggacagcca gggagtggcc
     5461 actgccgtgc cttctgtagt ccggctcagt tccagcaggt cgccgcgacc gtcaccgttg
     5521 aggtccgccg gcagcacgcg cgcgccgcga tcccgatggc ttgccggcag ggcgacaaag
     5581 cccgggccgt cgttgagcca gagggtgatc gcttcttcgc cttgttgatt ggtttgcgtc
     5641 ccgaccagat cggcgcggcc gtcaccgttg tggtcgagga agcgaaaatc ggtttcgcct
     5701 gggccaaggt caagcaccag atctggtcgc tgctccaggc gcatcgtggt cgtttccgcg
     5761 tccgcttcat cttttgtcaa ccagctttcg agcacccagc gcccgtcggc gttctgccac
     5821 actcgggcaa gatccatgcg aacatcacca tcaaggtcgg cagcgtgcca gggtgcgttg
     5881 gggtgcgcct cgccaagttc cagcgtgatt gcctggttgt cgtaggtcca tccccaggc
//
LOCUS       DAOSIT010000072         5905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12183_length_5905_cov_9.722222, whole genome shotgun sequence.
ACCESSION   DAOSIT010000072 DAOSIT010000000
VERSION     DAOSIT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12183_length_5905_cov_9.722222"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="prfA"
                     /locus_tag="PLL14_02885"
     CDS             <1..714
                     /gene="prfA"
                     /locus_tag="PLL14_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG02896.1"
                     /translation="SALFAGNLFRMYVRYAERRRWQVEVISASPSDLGGYREVIARVI
                     GNGVYSRLKFESGGHRVQRVPDTETQGRIHTSACTVAVMPEADALEEVQINPAEIRVD
                     TYRASGAGGQHINKTDSAVRITHLPTGIVVECQDDRSQHKNKAQALSVLVARIRDMQE
                     REQHASIASTRRNLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIEAVMDGDLDDLI
                     AALTAEHQADQLAMLAEGE"
     gene            698..>1392
                     /gene="prmC"
                     /locus_tag="PLL14_02890"
     CDS             698..>1392
                     /gene="prmC"
                     /locus_tag="PLL14_02890"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG02897.1"
                     /translation="MQKASEGSGERQRVLTGIGEAWRLASQSVDRLDARLLVEYVADC
                     THADLMAHPGRALSAVQFDRLVALVRRRAAGEPLAYLLGSAEFYGRRFLVSPAVLIPR
                     PDTERLVDLAIERMESLPGPRIVDLGTGSGVVAVTLKCLCPKALITAVDLSLPALEVA
                     RLNAGRHAAVVRFLAGDWYSPLAEACFDLIVANPPYVAEGDPHLLQDGLPFEPQTALT
                     DAVAGGDGLACIRA"
     assembly_gap    1393..1492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1493..1852
                     /locus_tag="PLL14_02895"
     CDS             <1493..1852
                     /locus_tag="PLL14_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02898.1"
                     /translation="QQLAYPRGPAGSQTFMVFVAPDGRLQRIEAVLDMEHFARIEPGK
                     SNKESVLRLIGPSVPQWTNYFKARDELVWEWRYCDAWNQLARLDVLFDATSGIVRTTY
                     QRPDLSGFDGVAPFCGH"
     gene            complement(1893..>3164)
                     /locus_tag="PLL14_02900"
     CDS             complement(1893..>3164)
                     /locus_tag="PLL14_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG02899.1"
                     /translation="KYELVRELGRGATSTVYLGNDRFTQRQVAVKVAFPEILKDPQRG
                     RLYTHIFLNEAALVGRLSHPHIVQTYDAVVDDHLCYIVMEYVAGGTLETVCSSNGLLP
                     YERVVEIIFKCTRALDFAFRAGITHRDIKPANILFTGVDPRQGDIKISDFGAAIIGSP
                     ERTLVLGIGSPAYMSPQQVRDQALDHQTDIYSLGVVMYQLLTGKLPFQARNSYDLIYQ
                     IINAEPRRPSALRSDIPASLDAIVARAMNKKLNKRYANWTEFAHDLAQAFRDRRLNLP
                     AEQMADFEKFDTLRSFAFFADFSDVEIWEVVRFSEWSRVSPGAMIIEDGMPGDCFYFL
                     AEGELKVLKNGMILDLLTTGECFGEMAVIGKATPRRGADVVAMTDTKLVRVAGSALQS
                     SSQACRMHFYQSFLGVLSERLSSANIRLVTF"
     assembly_gap    3165..3264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3265..4337)
                     /gene="argH"
                     /locus_tag="PLL14_02905"
     CDS             complement(<3265..4337)
                     /gene="argH"
                     /locus_tag="PLL14_02905"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOG02900.1"
                     /translation="MSQSDSSQYTWAGRFSEPTSDLVKRYTASVAFDQRMWRQDIRAS
                     LAHATMLARQGIISADDLAAIEHGMAQIVREIEAGTFVWSLDLEDVHLNIEKRLTTLV
                     GDAGKRLHTGRSRNDQVATDIRLYLRDATDDIVALLTEFQRRLLNLAEQEAATALPGF
                     THLQVAQPVTFGHHLLAYFEMTRRDSERFTDCRRRINRLPLGAAALAGTTYPIDRKLV
                     ADLLGFDGVCENSLDAVSDRDFAIEFCAAGALLMMHLSRLSEELVLWMNPCFGFITLA
                     DRFCTGSSIMPQKKNPDVPELARGKTGRVYGHLTALLTLMKGQPLAYNKDNQEDKEPL
                     FDTVDTLTDTLRVFAEMIPGITT"
     gene            4399..5433
                     /locus_tag="PLL14_02910"
     CDS             4399..5433
                     /locus_tag="PLL14_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOG02901.1"
                     /translation="MASIKQNLSTQPLPDFRNAGVMLRSLLGVNLLALVAALVQSDGV
                     TDSVQRYIDMAVWVQPLLLFDLTVLALLGNALRRCPVWVARALVLLLAVGSALLWSHL
                     WGLLGFDDGGWQRLLRAGLLGGVAAGSLLSYFALRASALSPALAEARLQSLTARIRPH
                     FLFNSLNAVISLIRLEPRRAESALEELAELFRALMRDHRELVPLAAEIALCRQYLNLE
                     KLRLGERLNVEWELADLPPDLDVPPLLVQPLLENAVYHGIEPAETRGTIRIRVTRNGD
                     ELHIDLANPCGGPAEHSRGNHMALDNIRERLALYYDLEARIDTGQVVLTDGQLEYRVR
                     IVLPCRVRSL"
     gene            5430..>5905
                     /locus_tag="PLL14_02915"
     CDS             5430..>5905
                     /locus_tag="PLL14_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG02902.1"
                     /translation="MSYATTLSVIIVDDEGPARARLRDLLADIAHELPNEIVAEADNG
                     LRALAAIAGAGVDVALVDIRMPGMDGIELALHLSQLEHPPAVIFVTAFDAYAVRAFEL
                     NAVDYLLKPVRAPRLLAALQKVPQSRALDPNRMVRLAQGPRTHLSYHERGRLLLIPV"
BASE COUNT         1054 a         1640 c         1834 g         1177 t
ORIGIN      
        1 tcggcgctgt ttgccggtaa tctgtttcgc atgtatgtac gctatgccga gcgaaggcgt
       61 tggcaagtgg aagtgatttc ggccagtcct tcggaccttg gggggtatcg ggaagtcatc
      121 gcccgcgtca tcggcaatgg cgtttactcg cggttgaagt tcgagtcggg tggtcatcgt
      181 gtgcagcgtg tgcccgatac cgagacgcaa ggccgcatcc atacttcagc ctgcacggtg
      241 gcggtgatgc ccgaggcgga cgccctggag gaagtgcaga tcaacccggc agagattcgc
      301 gtcgatacct accgcgcttc gggtgcggga ggacagcaca tcaacaagac cgattctgcg
      361 gtgcgtatta cccacctgcc gacgggtatc gtcgtcgaat gccaggatga tcgatcgcag
      421 cacaagaaca aggcgcaggc gctatccgtg ctggtggcgc gcatccggga catgcaggag
      481 cgcgagcagc acgcgagtat tgcctcgacg cgccggaatc tgatcggcag cggtgatcgc
      541 tccgagcgca tccggaccta caattttccg caggggcgcg tgaccgatca ccgtatcaac
      601 ctgaccttgt acaagatcga agcagtgatg gacggtgatc tcgacgacct cattgccgct
      661 ctgaccgctg agcatcaggc cgatcaactg gccatgcttg cagaaggcga gtgagggcag
      721 cggcgagcga cagcgtgtgc tgaccggtat cggagaggcc tggcggctgg ccagccagag
      781 cgtcgatcgc ctggatgcgc gtttgctggt cgaatacgtg gcggattgta cgcatgccga
      841 tctgatggca catcccgggc gagcgctgtc ggcggtgcag ttcgaccgac ttgttgcgct
      901 cgtccggcgg cgtgccgcag gcgaacctct ggcatacctg ctgggctctg ccgaatttta
      961 tgggcgccgt tttctggttt ccccggcggt gttgattcca cgcccggata cggagcggtt
     1021 ggtcgacctg gcgatcgaaa ggatggaatc cctgcctggt ccgcgcatcg ttgatctggg
     1081 gacaggttcg ggtgtggtcg cggtgaccct gaagtgtctg tgtccgaagg cattgatcac
     1141 ggcggtcgat ttgtcgctgc cagcgctgga agtggctcgc ctgaacgccg ggcggcacgc
     1201 cgcggtggtt cgctttcttg ctggcgactg gtactcgccg ctggctgaag cgtgtttcga
     1261 cctgatcgtt gccaatccac cgtacgttgc tgaaggtgat ccgcatttgc tgcaggacgg
     1321 tctgcctttt gagccgcaaa cggcgctgac cgatgcggtg gccggtggcg atggtctcgc
     1381 ctgcatccgg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagcagtt
     1501 ggcttatcca cgcgggccgg cagggagcca gaccttcatg gtcttcgttg cacccgacgg
     1561 acgactgcag cgcattgagg cggtactgga catggagcat ttcgcacgta tcgaacccgg
     1621 gaagagcaac aaggagtctg tgttgcgcct gatcggtccg tctgtcccgc agtggaccaa
     1681 ctacttcaag gcgcgcgatg aactggtctg ggaatggcga tactgcgatg catggaacca
     1741 gctcgcgcgc ctggacgtgc tgttcgacgc cacttccggc atcgtccgca cgacatatca
     1801 aaggccagac ctgagcggat tcgacggagt ggcgccattc tgcggccatt agcaccaggc
     1861 tggtttgccg cttgcccgtt cgtttcaggc tttcagaaag tcacaaggcg gatgttggcc
     1921 gaactcaggc gttcgctgag aacgccgaga aaagactggt agaaatgcat tcggcaggct
     1981 tgcgaagaac tctgcaaagc gctgcccgca accctgacca gcttggtatc ggtcattgca
     2041 acaacgtcgg caccacgtct gggtgtcgcc ttgccgatca ccgccatctc accaaagcac
     2101 tcaccggtcg tcagcaggtc aaggatcatg ccgttcttga ggactttgag ttccccttcg
     2161 gcaagaaaat agaagcagtc gccaggcatg ccgtcctcga tgatcatcgc accgggtgaa
     2221 acgcgactcc attcggaaaa acgcaccact tcccagattt cgacatccga gaagtcggca
     2281 aagaaggcga acgagcgcag cgtgtcgaac ttttcgaaat cagccatctg ctctgcgggc
     2341 aggttcagtc tcctgtcacg gaaggcctga gccagatcgt gtgcgaattc cgtccagttg
     2401 gcgtagcgtt tgttgagttt cttgttcatc gcacgtgcga cgatggcgtc aagcgaagcc
     2461 gggatgtcac tgcgcagcgc cgaaggcctg cgtggctcgg cgttgatgat ctggtagatc
     2521 aggtcatagc tgttgcgagc ctggaaaggc agtttcccgg taagcaactg atacatgacc
     2581 acgccgagag agtaaatgtc cgtctggtga tcgagcgctt gatcgcgcac ctgttgtggc
     2641 gacatgtacg ctggtgaccc gattcccagc accagtgtgc gttcgggtga gccaatgatg
     2701 gcagcgccaa aatcggagat cttgatgtcg ccctggcgcg ggtcgacgcc ggtgaacagg
     2761 atatttgccg gtttgatgtc ccgatgcgtg atgccggccc ggaaagcgaa atcgagcgct
     2821 cgcgtgcact tgaaaatgat ctcgacgaca cgctcgtagg gcaacaggcc gttcgatgag
     2881 cagaccgtct ccagggtgcc gcccgccaca tactccatga cgatgtagca caggtggtcg
     2941 tcgaccacgg catcgtatgt ctgaacgata tgcggatgtg acaatctgcc gaccagcgcc
     3001 gcctcgttga ggaagatgtg cgtgtatagg cggccgcgtt gaggatcctt caaaatttcc
     3061 ggaaaggcga ccttgaccgc cacctggcgt tgggtgaaac ggtcgttgcc gaggtaaact
     3121 gtggacgttg caccacggcc gagttctcgg acaagttcgt atttnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnctggtg gtgatgccgg gaatcatctc ggcaaaaaca
     3301 cgcagcgtat cggtgagggt atctaccgtg tcgaacagtg gttccttgtc ctcctggttg
     3361 tccttgttgt acgccagggg ctggcctttc atcagcgtca gcagcgccgt cagatgaccg
     3421 tagacgcggc cggtcttgcc gcgtgccaac tcgggcacgt cgggattctt cttctggggc
     3481 atgatcgaac tgccggtgca gaagcgatcg gcaagcgtga tgaagccgaa acaagggttc
     3541 atccacagca ccagttcttc cgacaggcgc gagagatgca tcatcaacag tgcgccggcg
     3601 gcgcagaatt cgatcgcaaa gtcgcggtcg gaaacggcat ccagcgagtt ttcacaaacg
     3661 ccgtcgaaac ccaggagatc ggcgaccagt ttgcggtcga tcgggtaggt cgtaccggcg
     3721 agggcagcgg cgccgagggg caggcgattg atacgccgcc ggcagtccgt gaagcgctcg
     3781 ctgtcacggc gcgtcatctc gaaataggcc agcaggtgat gcccgaaggt taccggttga
     3841 gcaacctgca ggtgggtgaa gccgggcagc gcggtcgccg cttcctgctc ggcgagattc
     3901 agcagccgcc tctggaactc ggtcagcagt gcgacgatgt cgtcagtcgc atcgcgcaga
     3961 tagaggcgaa tgtcggtcgc gacctggtcg ttgcgtgagc ggcccgtgtg caggcgcttg
     4021 ccggcatcgc cgaccagcgt tgtcaggcgt ttctcgatgt tcaggtgcac atcttcgagg
     4081 tccagtgacc aaacaaacgt gccggcttcg atttcgcgta cgatctgcgc catgccgtgc
     4141 tcgatcgctg cgaggtcgtc ggcagagatg atgccctgtc tggccagcat cgttgcatgc
     4201 gcgagcgagg cgcgaatgtc ctgccgccac atccgttggt cgaaggcgac tgaagccgtg
     4261 taacgcttga cgaggtcaga ggtcggctcc gagaagcggc cggcccacgt gtactgcgaa
     4321 gaatccgatt ggctcatgag ggacaggttc ctgggctaaa atcactcgta gaaccttgaa
     4381 caggcgccac ttcgagtgat ggcaagtata aagcaaaacc tgagcacgca gcccctgcca
     4441 gactttcgca atgccggagt catgttgcgc agcctgcttg gggtgaacct gctggccctg
     4501 gtcgcggcgc tggtgcagag cgatggggtg accgacagcg tgcagcgtta tattgacatg
     4561 gcggtgtggg tgcaaccctt gctgctcttc gacctgacgg tcctggcact actggggaac
     4621 gccttgcggc gctgtccggt gtgggtcgcg agggcgttgg tgctgctact ggccgtaggg
     4681 tccgcgctcc tctggtcgca tctctgggga ttgctgggct tcgacgacgg tggttggcag
     4741 cgcctgttgc gggccggtct gctcggtggg gtggccgcag ggtcgctgct gtcctacttt
     4801 gccttgcgcg ccagcgccct gtcgccggcg ctggcagaag cgcggctgca gtcgctgacg
     4861 gcacgcatcc ggccacattt cctgttcaac agcctcaacg ccgtgatctc cctgattcgc
     4921 ctggaacccc ggcgggccga gtcggcgctg gaggagttgg cggaactctt tcgtgccctc
     4981 atgcgtgacc accgggaact ggttcccctg gctgcagaga ttgccttgtg tcggcagtat
     5041 ctgaacctcg aaaaactgcg tcttggcgag cgactgaacg tggaatggga actggcggat
     5101 ttgccgcccg atctcgacgt gcctccgctg ctggtgcaac cgctgctgga gaacgctgtc
     5161 tatcacggca tcgaacccgc cgaaacccgc ggcacgatcc gcatccgtgt gacgcgcaac
     5221 ggcgacgaac tgcacatcga tctggcgaat ccatgtggtg gccctgccga acattcgcgg
     5281 ggcaatcaca tggcgctcga caacattcgc gaacgcctgg cgttgtacta cgatctggaa
     5341 gcacgcattg acaccggtca ggtcgtattg accgacgggc agctcgaata tcgggtgcgc
     5401 atcgttctac cttgccgcgt tcgttcctta tgagctacgc gactacactg tcggtgatca
     5461 tcgtcgacga cgaaggacca gcgcgtgcgc gcctgcgtga cctgttggcc gatatcgcgc
     5521 acgagttgcc gaacgaaatc gttgccgaag cagacaacgg cttgcgcgct ctcgccgcga
     5581 tcgctggcgc tggcgttgac gtggcgctgg tggacatccg catgccgggt atggatggca
     5641 ttgaactggc ccttcatctg tcgcaactcg agcatccccc ggcggtgatt tttgtgaccg
     5701 cctttgatgc ttacgccgtg cgcgcctttg aactcaatgc cgtcgattat ctgctgaaac
     5761 cggtgcgggc gccgcgtttg cttgccgcac tgcagaaagt cccgcaaagc cgagcgcttg
     5821 acccaaaccg gatggtgcgc ttggcgcaag gtccgcgtac gcacctctcg taccacgagc
     5881 gtggtcgttt gctgttgatt ccggt
//
LOCUS       DAOSIT010000073         5879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12257_length_5879_cov_9.275240, whole genome shotgun sequence.
ACCESSION   DAOSIT010000073 DAOSIT010000000
VERSION     DAOSIT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12257_length_5879_cov_9.275240"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1120
                     /gene="obgE"
                     /locus_tag="PLL14_02920"
     CDS             23..1120
                     /gene="obgE"
                     /locus_tag="PLL14_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG02903.1"
                     /translation="MKFIDEAKIVVAAGDGGNGIASFRREKYEPEGGPDGGDGGHGGN
                     VHVVADRNVNTLIEYRYVRRFRAKRGENGGSSDCYGKRGKDLTLRVPVGTLIADVNSN
                     EVLADLDADGKKVLLARGGRGGLGNVHFKSSVNRTPRQFTRGEPGEQRDLRLELRLLA
                     DVGLLGLPNAGKSTLLRAVSAARPKVADYPFTTLEPHLGVVRVDQDRSFVIADIPGLI
                     DGAAEGAGLGIRFLKHLARTRLLLHLVDLVPLDPDADPVRDVMTVVHELEKYDPALAG
                     KPRWLVLNKLDLIPEDEREQRITEFLLACNASIGETPCFRISAISGQGCRELMHKLQQ
                     ALDMLPTAVVAPTIAGPECFSDESFNAQPRP"
     gene            1123..2241
                     /gene="proB"
                     /locus_tag="PLL14_02925"
     CDS             1123..2241
                     /gene="proB"
                     /locus_tag="PLL14_02925"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOG02904.1"
                     /translation="MTITRIASARRLVIKVGSALVTNNGEGLDLGAISEWARQIATLH
                     ASGRQVILVSSGAIACGLQRLGWRKRPHAVHELQAAAAVGQMGLAQVYESAFARYGLH
                     TAQILLTHDDLADRKRYLNARSTLSTLLQFGVVPIINENDTVVTDEIKFGDNDTLGAL
                     VANLVEADCLIILTDQQGLFTADPRTDAAATLIGEARAGLPALEAMAGGVGTPFGKGG
                     MITKVIAAKRAARSGAHTVIASGRAADVIIRLANGEPLGTLLISETQALTARKQWLAD
                     HLQLNGQLILDNGAIQALREGKSLLPIGVLAVHGVFERGAAVACVDREGREIARGLAN
                     YGSSDARRIARHASQDIEDILGYMDEPEIIHRDNLILT"
     gene            2258..3367
                     /locus_tag="PLL14_02930"
     CDS             2258..3367
                     /locus_tag="PLL14_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOG02905.1"
                     /translation="MEQAPRSPVPPPAEIPAGGSLPRPTRLPLYLALAYVVLISYASL
                     YPLANWRDLGVSPLAFLDAGWPRYWTGFDLSINVLAYLPLGFLLTLALRHIPGGGWTA
                     AIVALLLGSLLSFSMESLQTWLPARVPSNLDLACNALGTAIGAVIALWNGKRIFRRIG
                     QMQQGLLAPIANAEVGLVLLGTWLLTQLSPETLLFGTGDLRNVLGITPAVPYAAPSFF
                     VLETCVIACNTLVIGLFARTLLADRTSMHLVLVAFFVLALAIRTLAAAVLVAPQDAFA
                     WLTPGARLGLLIGGVLLSLMLLLPASWRVALAGLALLAGTALVNLTPANPYSEAALAA
                     WRQGHFLNFNGLTRWVASLWPFVALPYLTAVGRRL"
     gene            complement(3368..4402)
                     /locus_tag="PLL14_02935"
     CDS             complement(3368..4402)
                     /locus_tag="PLL14_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOG02906.1"
                     /translation="MSTCEDPFVILGVHREASLDEIKRAYRRLAMFWHPDRNPAAVAG
                     NEFKRVHAAYELLLDPQRLAEWQQAQATTMTTAGGTATGNAADDLTQVLTLSLEEAAQ
                     GCRKDIELVHSVRCDTCSGSGRVQHNHSVPCRRCNGCGRLVRKGGGTNRCEGCNGRGY
                     LRETVCPECVSGWRMEPRILSVTVPPGLRDGERLRLPRQAPAKPAGDGHGAGDLYLKV
                     SLATHPLFVLDGRDLHCEVPVSVLRLLCGGCIEVPTLSGTASLDLSPAQGMLEQRLSG
                     LGFPGKRGRGAGDLIVHLQSIQMQELGPQDIEFLEGLQARLATDLERHAPRLAAWELQ
                     LRARRRQEQG"
     gene            4614..4922
                     /locus_tag="PLL14_02940"
     CDS             4614..4922
                     /locus_tag="PLL14_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG02907.1"
                     /translation="MSYFKHHVFFCCNQRGEGETCCNNAGATAAQTYAKDRIGELRLK
                     GAGKVRINKAGCLDRCDQGPVLVVYPEGVWYSYVDQEDIEEIIQEHLVQGRVVERLRI
                     "
     gene            4989..5627
                     /locus_tag="PLL14_02945"
     CDS             4989..5627
                     /locus_tag="PLL14_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG02908.1"
                     /translation="MRLQAEQLLIDGPVGKIDLIVENPGAPRGIALIAHPHPLFGGGN
                     TNKVVQTLARTFTHLDYVALRPNFRGVGRSEGQHADGQGETEDLLAVLAEAKCRYGNL
                     PVALAGFSFGAYVLTRVAEALLDAGHPAQRLVLVGTASGFVEGARRYHTKAVPRDTIV
                     IHGSEDTTVPLANVLEWAKPLELPVVVVPGADHFFHRRLHVIREIVSHAWRH"
BASE COUNT         1065 a         1956 c         1815 g         1043 t
ORIGIN      
        1 gcaccatgga agaaatccgc tcatgaaatt tatcgacgag gcgaagattg tggttgccgc
       61 cggggacgga ggcaacggca tcgcctcgtt tcggcgcgag aaatacgagc ctgagggtgg
      121 cccagacggc ggtgacggcg gccacggcgg caacgtgcac gtcgtcgccg accgcaatgt
      181 caacaccctg atcgagtatc gttacgtgcg caggtttcgc gccaagcgcg gggaaaacgg
      241 gggttcaagc gactgctacg gcaagcgcgg gaaggatctg acgctgcgcg tgccggtcgg
      301 cacgctcatc gccgatgtca acagcaacga agtgcttgcc gacctcgacg ccgacggaaa
      361 gaaggtgctg ctggccagag gtggccgcgg aggcctcggc aatgtccatt tcaagtcgag
      421 cgtgaatcgc acgccgcgac aattcacccg cggtgaaccc ggcgaacaac gcgatttgcg
      481 actggaactg cgcctgcttg ccgatgtcgg gctgcttggc ctgcccaacg ccggcaaaag
      541 cactttgctg cgcgcggtgt cggctgcccg gccaaaggtc gccgattatc cgttcacgac
      601 tctcgaaccc cacctgggtg tcgtgcgtgt ggatcaggac aggagcttcg tcatcgccga
      661 cataccggga ctgatcgacg gagccgccga aggggccggc ctgggaattc gcttcctgaa
      721 acacctcgca cgcacgcgtt tgctgctgca cctcgtcgac cttgtcccgc tcgacccgga
      781 tgccgacccc gtccgcgatg tgatgacggt cgttcatgaa ctggaaaaat acgatccggc
      841 actcgccggc aagccgcgct ggctggtcct caacaagctg gatctcattc ccgaggatga
      901 gcgcgaacag cgcattaccg agttcttgct ggcctgtaat gcgagcatcg gggaaacgcc
      961 gtgcttccgc atcagcgcga tcagcggtca gggttgccgc gagttgatgc acaagcttca
     1021 gcaagccctc gacatgttgc cgacggcggt ggtcgcgccg acgattgctg gcccggagtg
     1081 cttttcagac gagtcgttca acgcccaacc gcgcccctga acatgaccat cacccgtatc
     1141 gcttctgctc gccggctggt catcaaggtg ggctccgccc tggtcaccaa caacggtgaa
     1201 ggccttgatc tcggggcgat cagtgagtgg gcacgacaga tcgccacctt gcacgccagc
     1261 ggcaggcagg tcattctcgt gtcatcgggg gcgatcgcct gcggtctgca acgtctcgga
     1321 tggcggaaac ggccacacgc cgtacatgaa ctgcaggctg ccgcggcggt cggccagatg
     1381 ggactggcac aggtctatga gagtgctttt gcgcgctacg ggctgcatac ggcgcagatt
     1441 ctgctgaccc atgacgacct tgccgaccgc aagcgctacc tcaatgcacg ctcgacgctg
     1501 agcacgctcc tccagttcgg cgtcgtgccg atcatcaacg agaacgacac ggtcgtcacc
     1561 gatgaaatca agtttggcga caacgacacc ctcggagcgc tggtcgccaa cctcgttgaa
     1621 gccgattgcc tgatcatcct caccgatcaa cagggcctgt tcaccgcgga tccccgcacc
     1681 gacgcggcag ccaccctgat cggcgaggca cgcgccggcc ttcccgctct tgaggccatg
     1741 gctggcggtg tcggtacgcc attcggcaaa ggcggcatga tcaccaaggt gatcgccgcc
     1801 aagcgcgcag cccgcagcgg cgcccacacg gtcatcgcca gcggccgtgc cgccgatgtg
     1861 atcatccgcc tggccaatgg tgaacccctg ggcacgctgt tgatctctga aacgcaggcg
     1921 ctgacggcgc gcaagcaatg gctcgccgat cacctgcagc tcaatggtca actgattctc
     1981 gacaatggcg ccatccaggc attgagagaa ggaaaaagcc tgctgcccat cggcgtactg
     2041 gccgtgcacg gcgtttttga acgcggcgcg gcggtcgcct gcgtcgaccg cgaaggccgc
     2101 gagattgcgc gcggcctggc caactacggc agcagcgatg cgcggcgaat cgcccgccat
     2161 gccagccagg acattgaaga catcctgggc tacatggacg aaccggaaat catccatcgt
     2221 gacaacctga tcctgaccta gcaaacacgc acccctgatg gagcaagcac cgcggtcacc
     2281 cgttccgcca cctgcagaga tacccgccgg gggatcgctg ccgaggccca cacggctgcc
     2341 gctgtatctg gcactggcct acgtggttct gatcagctac gccagcctct accctctcgc
     2401 caactggcgc gacctcggcg tctcgccgct ggcttttctg gacgccggct ggcctcgcta
     2461 ctggacgggc ttcgacctct cgatcaacgt ccttgcctat ctgccgctgg gctttctgct
     2521 gacgctggcg ctgcgccaca ttcccggcgg cggctggact gcggccatcg tcgccctgtt
     2581 gctgggcagc ctgctcagct tcagcatgga aagcctgcaa acctggctgc cggcacgcgt
     2641 gccatcgaac ctcgatctgg cctgcaatgc cctgggcaca gcgatcggtg ccgtgatcgc
     2701 gctgtggaat ggtaagcgca ttttcaggcg catcggtcag atgcagcagg ggttgctggc
     2761 cccgatcgcg aacgcggagg tgggtctggt gctgctcggc acctggttgc tgacccaact
     2821 gtcaccggaa acactgctct tcggcaccgg cgacctgcgc aatgtgctcg gcatcacccc
     2881 ggccgtgccc tatgcagccc cgtccttctt cgtgctcgaa acctgcgtca ttgcctgcaa
     2941 caccctggtc atcgggctgt ttgcacgcac cctgctggcc gatcggacgt ccatgcatct
     3001 ggtcctggtg gctttcttcg tgctcgccct ggccatcagg acgctggccg ctgccgtact
     3061 cgtcgcgccg caggacgcct tcgcctggtt gacccctggt gcccgcctgg gcttgctgat
     3121 cggcggcgtt ttgctctcac tgatgttgct gctgccggct tcctggcgtg tcgcgctggc
     3181 cgggcttgcc ttgctggccg gcaccgcact ggtcaatctg acgccggcca atccctattc
     3241 ggaagctgcc ctggccgcct ggcgacaggg acacttcctg aacttcaacg gcctcacccg
     3301 ctgggtagcc agcctctggc ccttcgtcgc cctgccctac ctgacggcag tcgggcgacg
     3361 cctgtaatca gccctgttcc tgccgtcgac gcgcgcgcag ttgcagttcc caggcggcca
     3421 gtcgcggcgc atgccgctcg aggtcggtcg ccaaacgtgc ctgcaaacct tccagaaact
     3481 cgatgtcctg cgggcccagc tcctgcatct ggatactctg cagatgcacg atcagatcgc
     3541 cggcaccgcg cccccgcttg ccgggaaaac ccagcccgga aagacgttgc tcaagcattc
     3601 cctgagccgg agacaaatcg agactggcgg tgccgctcaa ggtcggcact tcgatgcagc
     3661 ccccgcagag cagccgcaaa acgctcaccg gcacctcaca gtgcagatcg cgcccgtcca
     3721 ggacgaacaa cgggtgtgtg gcgaggctga ctttgaggta caagtcgccg gcgccgtggc
     3781 catcgccggc cggcttcgcc ggcgcctgcc gaggcagccg caagcgctcg ccatcgcgta
     3841 gccctggcgg gaccgtgacc gacaggatac gcggttccat gcgccagcca ctgacgcact
     3901 ccgggcacac cgtctcgcgc aggtagcctc gcccattgca gccttcgcaa cgattggtgc
     3961 ccccgccctt gcgcaccagg cgaccacagc cgttgcaacg ccggcaggga acggaatggt
     4021 tgtgctggac ccggccactg ccgctgcagg tgtcgcagcg cacgctgtgc accagctcga
     4081 tatccttccg gcaaccctgt gccgcctctt cgagcgacag tgtcaggacc tgcgtcaggt
     4141 catcggctgc gttcccggta gccgtaccgc ccgcggtagt catcgtggtc gcctgcgcct
     4201 gctgccactc ggcaaggcgt tgcggatcga ggagcagttc gtaagccgca tggacgcgct
     4261 tgaactcgtt cccggcaacc gccgcagggt tgcgatccgg atgccagaac atggccagcc
     4321 ggcgatacgc ccgcttgatt tcgtcgagcg atgcttcgcg gtgtacgcca aggatgacga
     4381 aggggtcctc gcaagtgctc atggaatcgt cgtggtggta gccgttcaga ccgaccgtgt
     4441 ccgaggacac gaaagaagac cgcggaggcg atcgaccagg cattctacgt gatcaaagct
     4501 ccgctccaca gatctctgtc agtagcgcag ccgcaacgcg cagctgatcg tcgttgaaac
     4561 agggttaaaa tcgaccacat ctgcaagcaa cccatcaaca ggagccggtt cgtatgagct
     4621 atttcaagca tcatgtgttt ttctgctgca accagcgggg cgagggggaa acctgctgca
     4681 acaacgccgg cgcaaccgca gcacagacct acgccaagga tcgcatcggc gaactgcggc
     4741 tcaagggtgc cggcaaggta cggatcaaca aggccggctg cctcgatcgc tgcgatcagg
     4801 gaccagtgct ggtggtctat ccggaaggcg tgtggtacag ctacgtcgac caggaggaca
     4861 tcgaggaaat catccaggag cacctggtac aaggacgagt cgtcgaacgg ctgcgcatct
     4921 gaagggaaaa gcgccgtcgg ctgcagccct gcccggcaga gtgaagtcag caagaggaaa
     4981 ccgaagccat gagactgcaa gcagaacaac tgctgatcga cggaccggtc ggcaagatcg
     5041 acctgatcgt cgagaatccg ggcgcgccgc gcggcatcgc cttgatcgcc cacccgcatc
     5101 cgctgttcgg cggcggcaat accaacaagg tggtacagac gctggcgcgc accttcaccc
     5161 atctcgatta cgtggcgctg cgcccgaatt tccgcggcgt cggtcgcagc gagggacagc
     5221 acgccgatgg tcagggggaa accgaggacc tgctggcggt actggcggaa gccaagtgtc
     5281 gttacggcaa tctgccggtc gccctcgccg gcttctcttt cggcgcctac gtcctgaccc
     5341 gcgtggccga agcgctgctc gacgccgggc atccggcgca gcggctggtg ctggtcggca
     5401 ccgcctcggg cttcgtcgaa ggcgcacgcc gctaccatac caaggccgtg ccacgcgaca
     5461 ccattgtcat ccatggatcc gaagacacca ccgtgccact ggccaacgtc ctcgaatggg
     5521 ccaagcccct ggaattgccg gtggtggtcg tccccggcgc cgatcacttc tttcaccggc
     5581 ggctacatgt gatccgcgag atcgtcagcc atgcgtggcg ccattgacgg ctggcgctgc
     5641 gattgaagtg ccatggagaa cactgcgagc ggtttttcgg acacgccagt acttggaggg
     5701 tcaccttcac ccgctctcct ccgcctcgaa tctggcgatc ctgcagattg caccggcctg
     5761 ccgcctgcgg cggcgaatca gggcgggtca tcgtgctccc cttcgctgac ggggagggct
     5821 tgcgattcaa ttgcctttca ggcggtcgag aacgaatttc gtgaccgggc cagcgatgc
//
LOCUS       DAOSIT010000074         5864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12300_length_5864_cov_8.302978, whole genome shotgun sequence.
ACCESSION   DAOSIT010000074 DAOSIT010000000
VERSION     DAOSIT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12300_length_5864_cov_8.302978"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLL14_02950"
     CDS             complement(<1..275)
                     /locus_tag="PLL14_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOG02909.1"
                     /translation="MSGGTEGESASLSPAASLPLWLAHLESLHPRGQGGIELGLERVL
                     RVKDELRQHPECPVITVGGTNGKGSTCAYLEAIYSLAGFRVGCYTSP"
     gene            complement(286..1158)
                     /gene="accD"
                     /locus_tag="PLL14_02955"
     CDS             complement(286..1158)
                     /gene="accD"
                     /locus_tag="PLL14_02955"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOG02910.1"
                     /translation="MGWLNKLLPPKIKRNDNGVARRSSLPEGLWSKCVACEAVLYATD
                     LASNCNVCPKCGHHKRLDARARIELLLDAEGRSEIASEVLPVDSLSFKDSRRYPERLQ
                     DATVATGETEALVAMRGAIRTLPVVLAVFEFDFMGGSMGSVLGERFVRAVQAAVDDAV
                     PFICVTASGGARMQEGLFSLMQMAKTTAILTRLSQARLPFISILSDPTMGGVSASFAF
                     IGDVVIAEPGALIGFAGPRVIEQTVRETLPEGFQRSEFLLEKGAIDMIVDRREMKERV
                     AAVLAMLQKRPALQ"
     gene            complement(1146..1979)
                     /gene="trpA"
                     /locus_tag="PLL14_02960"
     CDS             complement(1146..1979)
                     /gene="trpA"
                     /locus_tag="PLL14_02960"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOG02911.1"
                     /translation="MKRIQAVFERLQAQGRKALIPFITAGDPEPALTVPLLQTLVRAG
                     ADIIELGVPFSDPMADGPTIQRASERALAKGVSLRQVLGLVRDFRAADQETPIVLMGY
                     ANPIEAMGVEAFAATAGAAGADGVLVVDYPPEEGIDFAHTLRRHGLAPIFLLAPTSSA
                     ARIAQVGALAGGYIYYVSLKGVTGSAALDVAAVAARIPDIRARTGVPVGVGFGIRDAA
                     TAAAVAKIADAVVVGSRIIEEIEQSVPAQACANVLALVAEIRRGIDAATSTSGGTTWA
                     G"
     gene            complement(1982..3187)
                     /gene="trpB"
                     /locus_tag="PLL14_02965"
     CDS             complement(1982..3187)
                     /gene="trpB"
                     /locus_tag="PLL14_02965"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOG02912.1"
                     /translation="MANYAHDLPDASGHFGPYGGIFVAETLITALAELNEAYAVARHD
                     PQFVAEFEYELKHYVGRPSPVYHARRWSELLGGAQIYLKREDLNHTGAHKINNCIGQA
                     LLARRMGKPRVIAETGAGQHGVATATVAARYGMTCVVYMGAEDIRRQAANVYRMKLLG
                     ASVVPVNSGSRTLKDALNEAMRDWVTNVSDTFYIIGTVAGPHPYPMMVRDFQSVIGRE
                     SIAQMHEHCGRQPDAVIACVGGGSNAMGIFHPYLPFEGVRLIGVEAAGEGLTTGRHAA
                     SLTAGRPGVLHGNRTYLLQDENGQIIETHSISAGLDYPGVGPEHAWLKDSGRAEYVSV
                     SDVEALQAFHDLCRLEGIIPALESSHALAYAGKLAPTLDKDRILLVNLSGRGDKDMHT
                     VAEKSGIKF"
     gene            complement(3336..>3929)
                     /locus_tag="PLL14_02970"
     CDS             complement(3336..>3929)
                     /locus_tag="PLL14_02970"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOG02913.1"
                     /translation="VHAGADAIGLVFYPPSPRYVDLPTAVRLARAVPPFVSIVGLFVN
                     ADPLAVRATLAAVPIHLLQLHGDEDEAYCRQFDRPYIKAARVRPGMDLLQYAAAFPGA
                     QAILLDAFVDGYGGGGRVFDWSLVPAALGRSLILSGGLDAENVTAAVCRLRPEAVDVS
                     SGVEASKGIKDAGKMRAFVAAVRAAGSMSGDGCEKKS"
     assembly_gap    3930..4029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4030..4622)
                     /gene="truA"
                     /locus_tag="PLL14_02975"
     CDS             complement(<4030..4622)
                     /gene="truA"
                     /locus_tag="PLL14_02975"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG02914.1"
                     /translation="MRLALAVEYDGSSFRGWQQQPGGGTVQDALQDALRQFAGVPVSV
                     VCAGRTDAGVHALGQVVHLDTQLERSMFAWVRGANTFLPAAVAVRWAQIVANDFHARA
                     SAYSRHYRYLLINRAHRTGVWHGKAGWYHHPLDVAAMQTAADLLLGERDFSAFRAAEC
                     QAISPVKILRRAEVRRFDDLIVFDFEASAFLHHMVRNL"
     gene            complement(4626..5150)
                     /locus_tag="PLL14_02980"
     CDS             complement(4626..5150)
                     /locus_tag="PLL14_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02915.1"
                     /translation="MAAAFLVPPLFGRLALQRAIRLARGARWLFLSLFVILAWGRAGE
                     PVWGGAMAPSAEGLSEALTQIGRLLLVLMAVAVLRQRLSVTEFLTAAHRLLWPLRRCG
                     VDTDRGIVRVLLVLQYLETTPRQRDWRRLLEVPDVSSSEVFELSDRHFPGRDYLIIGI
                     LLVSVALLYGYAQA"
     gene            complement(5322..>5864)
                     /locus_tag="PLL14_02985"
     CDS             complement(5322..>5864)
                     /locus_tag="PLL14_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOG02916.1"
                     /translation="PGESSWGVTTLTRAETDEEVLDIGSPNAEALDEALEFDVRLTES
                     TVLGEAMQHPAFDMSSISLDLAEPDIEDRAEASNPSISPEFSFDADQEDTLVNPNFSV
                     QQSQTEPNEVFDFATASDVGAESLISAGEEVATKMDLARAYQEMGDIEGARELLQEVA
                     NEGDAAQRGAARALLAGLRD"
BASE COUNT         1162 a         1952 c         1703 g          947 t
ORIGIN      
        1 tgcggcgagg tataacagcc gacccgaaaa ccggcaaggc tgtagatggc ttccaggtag
       61 gcgcaggtcg accctttgcc gttggtgccg ccgacggtga tcaccggaca ttccgggtgc
      121 tggcgcaact cgtccttgac gcgaaggaca cgttccagcc ccagttcgat ccctccctgc
      181 ccgcgcggat gcagactttc gaggtgggcc agccagagcg gcagggacgc agccggcgag
      241 agcgacgcag attcgccctc ggtgccgccg ctcaaagccc gcgcctcact gcagtgccgg
      301 ccgcttctgc agcatggcca gcaccgctgc aacccgttcc ttcatttcgc gccgatcgac
      361 gatcatgtcg atggcgccct tctccagcag gaattccgag cgctggaaac cctccggtaa
      421 agtctcgcga accgtctgtt cgatgacccg cggacccgca aaaccgatca gcgcccccgg
      481 ttcggcaatc accacgtcgc cgatgaaggc gaacgacgcc gatacacccc ccatcgtcgg
      541 atcgctgagg atcgagatga agggcagccg ggcttgcgaa aggcgagtca ggatcgccgt
      601 cgtcttggcc atctgcatca gcgaaaacag tccttcctgc atgcgcgcac cgccagaggc
      661 agtcacgcag atgaaaggca cggcatcgtc gaccgccgcc tgcacggcgc gcacgaaacg
      721 ctcacccagc accgatccca tcgacccacc catgaagtcg aactcgaaga cggcgagcac
      781 taccggcaag gtcctgatcg caccacgcat cgccaccagc gcttcggtct cgccggtcgc
      841 cacggtggcg tcctgcaggc gctccggata gcggcggctg tccttgaatg acaaggaatc
      901 gaccggcagc acctcggagg caatctcgct gcgcccctcg gcatcgagca gcagttcgat
      961 ccgggcgcgc gcgtccaggc gcttgtggtg accgcacttg gggcagacgt tacagttgct
     1021 cgccaggtcg gtcgcgtaga gcaccgcctc gcaggctacg cacttgctcc acaggccttc
     1081 gggcagtgat gaccggcgcg caacaccgtt gtcgttgcgc ttgattttcg gcggcagcag
     1141 tttgttcagc cagcccatgt cgttcctcct gacgtactcg ttgccgcgtc tattccgcga
     1201 cgaatctccg ccaccagtgc gagcacgttc gcacacgcct gcgccggtac agattgctca
     1261 atttcttcga tgatccgact gccgacgacc accgcatcag caattttcgc caccgctgcg
     1321 gcagtcgccg catcgcggat gccgaagccg acaccaaccg gcacgccggt acgtgcccgg
     1381 atgtccggga tgcgggcggc aaccgcagcg acgtcgagcg ctgccgatcc ggtaacccct
     1441 ttcagcgaaa cataatagat gtagccaccg gcaagtgctc cgacctgcgc aattcgcgcc
     1501 gcgcttgacg tcggcgccaa caggaagatc ggagcgagtc catggcgccg cagcgtatgc
     1561 gcaaaatcga tcccttcctc ggggggataa tcgaccacca gcacgccatc ggcgccggca
     1621 gcgccggcgg tagccgcaaa ggcctcgacg cccatcgctt cgatcgggtt ggcataaccc
     1681 atgagtacaa tcggtgtctc ctggtctgcg gcacggaagt cgcgcaccag cccgagcacc
     1741 tgacggagac tgacaccttt cgccaacgct cgctcggagg cgcgctgaat ggtgggaccg
     1801 tcggccatcg gatcggagaa cggaacgccc agctcgatga tatcggcacc ggcgcggacc
     1861 agggtctgca gcaagggcac ggtcaacgcc ggttcggggt caccggcggt gatgaacggg
     1921 atcagcgcct tgcgcccctg cgcctgcaga cgctcgaata cagcctgaat tcttttcatg
     1981 gtcagaattt gatgcccgat ttttcggcca cggtatgcat atccttgtca ccccgaccgg
     2041 agaggttgac cagcagaatc ctgtccttgt ccagggtcgg cgccagcttg ccggcgtagg
     2101 ccagggcatg gctagactcc agtgccggga tgatgccttc gagccgacac aagtcgtgaa
     2161 aagcctgcag cgcctcgaca tcgctgacac tgacgtactc ggcccgacca ctgtccttga
     2221 gccacgcatg ttccgggccg acacccggat aatcgagccc ggccgagatc gagtgcgtct
     2281 cgataatctg accattttcg tcctgtaaca ggtaagtccg gttgccgtgc agcacacctg
     2341 gacgaccagc cgtcaatgaa gcggcgtgac gtccggtggt gaggccttca ccagccgcct
     2401 cgacaccgat cagacgcaca ccctcgaacg gcaggtaggg atgaaaaatc cccatcgcat
     2461 tggaaccgcc acccacgcag gcaatcaccg catcgggctg acgaccgcag tgttcgtgca
     2521 tctgggcaat cgactcgcgg ccgatgaccg attgaaaatc gcgcaccatc atcgggtaag
     2581 gatgtggacc agcgacggtg ccgatgatgt agaaggtgtc ggagacgttc gtcacccagt
     2641 cgcgcatggc ttcgttcaag gcatccttga gcgtcctgga accgctgttg accggcacca
     2701 cgctggctcc gagcagtttc atccggtaaa cattggccgc ctgccgccgg atgtcttcgg
     2761 cgcccatgta gaccacgcaa gtcatgccat agcgtgcggc aacggtggcc gtggcgacgc
     2821 cgtgctggcc ggcacccgtt tcggcgatga cgcgcggctt gcccatccgt cgcgccagca
     2881 gcgcctggcc gatgcaattg ttgatcttgt gcgcgccggt atggttcagg tcttcacgct
     2941 tcaggtaaat ctgcgcaccg ccgaggagct cagaccagcg cctggcgtga tagaccggac
     3001 tgggccgacc gacatagtgt ttgagttcgt actcgaattc ggcgacgaac tgcgggtcgt
     3061 gccgcgcgac ggcgtaagct tcattgagct cggcgagcgc agtaatcagc gtttccgcca
     3121 cgaagatgcc accgtagggg ccgaaatggc cgctggcatc aggtaagtca tgggcataat
     3181 tggccatcaa caatctcctt gcgacgagtt acgactggac gctgctacag cgcccggggg
     3241 aaaccctcag tcgtcgcctc aggtatgttt agccacgtgg cggcgccagc acgtccgcgt
     3301 ccgtccacca gtcttcctgc gcatctgcca ttccgtcaag acttcttctc gcagccgtcg
     3361 ccgctcatcg acccagccgc acgcactgcc gccacaaagg cacgcatctt cccggcatcc
     3421 ttgatgcctt tgctcgcctc gacccctgac gagacatcca ctgcctcggg tcgcaaccgg
     3481 cacaccgccg cggtcacgtt ctccgcatcc agcccgccgg acagaatcaa cgacctgccc
     3541 agcgcagcgg gcaccagcga ccagtcaaaa actctgccgc cgccaccgta gccatcaacg
     3601 aaggcatcga gaaggatcgc ctgcgcaccg gggaaggcag ccgcgtattg tagcaaatcc
     3661 attcccggcc tgacgcgcgc ggccttgatg taaggtcggt cgaactgccg gcaataggcc
     3721 tcgtcttcat caccatgcag ttgcagcagg tgaatcggca ccgcagcgag agttgcccgc
     3781 acggcgagcg gatcggcatt gacgaagaga ccgacaatgc tgacaaaggg cggcacggca
     3841 cgcgccaacc gaaccgccgt cggcagatcg acgtaacgcg gactcggtgg gtagaatacc
     3901 aggccaatgg catccgcccc ggcgtgcacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnna gattacgcac catgtggtga aggaaggccg aggcctcgaa atcaaagacg
     4081 atgagatcgt caaaacggcg gacctcggca cggcgcaaga tcttcaccgg cgaaatggcc
     4141 tgacactcgg cggcgcgaaa ggcggaaaaa tcgcgctcac cgagcaacag gtccgccgct
     4201 gtctgcatcg ccgcaacatc cagtggatga tggtaccaac cggccttgcc gtgccagacc
     4261 cccgtccggt gcgcacggtt gatcaacaga tagcgatagt gccgcgagta cgccgaagcg
     4321 cgtgcatgaa agtcattcgc cacgatctgt gcccagcgca cggccactgc cgccggcagg
     4381 aaagtgttgg cgccgcgcac ccaggcaaac atcgagcgct ccaactgggt atcgagatgc
     4441 accacctgcc ccaacgcatg cacaccggca tcggtacggc cggcgcagac cacggatacc
     4501 ggcactccgg cgaactgccg caaggcgtcc tgcaacgcat cctggacggt gccgccgccc
     4561 ggttgttgct gccagccacg aaagctgctg ccgtcgtact ctaccgccag ggcaagtctc
     4621 atcgctcacg cctgtgcata gccgtagagg agcgcgacac tcaccagcag gataccgata
     4681 atcaaatagt cgcggccggg aaagtggcga tcgctcaact cgaacacctc gctgctgctg
     4741 acgtcgggca cttcgagcag ccgccgccag tcgcgctgcc gtggtgtcgt ctcaaggtac
     4801 tgcaggacca gcagtacccg aacaatcccg cgatcggtat cgacgccgca gcgccgcagc
     4861 ggccacagca gacgatgcgc ggcagtcagg aactccgtca cggacaaacg ctggcgtagc
     4921 accgctaccg ccatcaatac cagcaacaag cgcccgattt gcgtcaaggc ctcgctcaaa
     4981 ccctcggcac tgggcgccat ggcaccgccc cagacgggct cgccggctcg accccaggca
     5041 agaatgacga acagggacag gaacaaccaa cgcgctcccc tggcaagccg gatggcacgt
     5101 tgcaaagcca gacgaccgaa cagcggcggt accagaaagg ccgccgccaa caagctgccc
     5161 tccaggccct gtacggccac caaactcagg agccagacaa ctagacaggt ggtcgggtgc
     5221 acaaggcagc gttctcgttg agcgcccacg atccgcacag gaacgagccg caggcacgga
     5281 ggcgcggcga cccgttcggt gctcgatccc aggcatgcgg attagtcgcg caatccggca
     5341 agaagggctc gcgcggctcc acgttgcgcc gcatccccct cattggcgac ctcctgcaag
     5401 agctcgcgag cgccctcgat gtcgcccatt tcctgatacg cccgcgccag gtccatcttg
     5461 gtggccacct cttcgccggc actgatcagg gactccgctc cgacgtcaga agccgtggcg
     5521 aagtcgaaca cctcgttcgg ctcggtttgt gactgttgta ccgaaaagtt cgggttgacc
     5581 agggtgtcct cctggtcggc atcgaaggaa aactccgggg aaatactcgg atttgacgcc
     5641 tccgcacgat cctcgatgtc gggctcagcc agatccaggc tgatcgacga catgtcaaag
     5701 gctggatgct gcatcgcctc gccgaggacc gtcgactcgg tcaaccggac atcaaactcc
     5761 agcgcctcat ccagagcctc cgcattcggc gaaccgatgt cgagtacctc ctcatcggtt
     5821 tcggcgcgcg tgagcgtcgt taccccccag ctcgactcgc cagg
//
LOCUS       DAOSIT010000075         5793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12479_length_5793_cov_10.572499, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000075 DAOSIT010000000
VERSION     DAOSIT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12479_length_5793_cov_10.572499"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLL14_02990"
     CDS             complement(<1..347)
                     /locus_tag="PLL14_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOG02917.1"
                     /translation="MQRRRFLATPLLLPTLTWPAEALLAYPPVTPGPGLAFPRDHGGH
                     PDFRTEWWYVTGALDSPQPDIGFQLTFFRNRPGMAETLRSPLAARQILWAHAALTVPG
                     ERLRHTERAARANL"
     gene            complement(365..2878)
                     /locus_tag="PLL14_02995"
     CDS             complement(365..2878)
                     /locus_tag="PLL14_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02918.1"
                     /translation="MNRLSWWLILAQFRTRRAATLLSMLAIALGVALGYAIHLINDAA
                     LADFSRAMQTVQGNPDAVLAARDSAGSVPLASINEIARDPAVLLVAAVIESRVRLDSS
                     PTPLRLIGIDVFSAAALMPRLLPRHDGRAGSAHILAGGVYASPALLEKFGLHPGSAVT
                     LQRGDQDWHAIIAGDLPATGPDDLLLVADIAWVQEHFGPANAVSEGRIRLGPDTDPAA
                     WRTALEQRLPSGLLLRMADDDGVRVSNLSRAYRVNLNVLALVALLTGAFLVFATQLSA
                     VAQRSTQFALLGVLGLSPRMRLLQVLLEGLAIGVPGALLGLGLGYLLALAFTQVLGGD
                     LGGGYFTGSAPIIVPAPVPALGFLALGCAASLAGASYPAYLNRQQALAQALQTGFAQR
                     PRGSDPTATSQPLLPALLAIGALALTQAPPWFDLPLGGYAAIGLVLLIGIASAPPLTR
                     QVFGHLAGRALPAPQQIALQNVAQAPLLAQVAASGLIVSFALTASMVIMVSSFRVAVD
                     RWLDDILPAPLYLRSRATPLPAEVLTALLQADTPFARVERMAQGSLTIDAQRPNVALL
                     VREIERGNPAARLPFSGELVAAPPDMAVVWISEALHEIYRIVPGQILHLPLLEREVEV
                     FVGGVWRDYSRQFGALVISREDYEHLGGRFQPTDLALWPRTGQEAAAATWLAPYAGRH
                     GLELSEAAEIRRLSLSIFDKSFAVTYALEAAAMLIGLFGLAVTLAASVWLRARELATL
                     GALGFDRRMLSQAVMLEGALIACVGLLVGLACGVAIGAILTHVINPQSFHWRMPLSIP
                     WPTLLAGAGLTLAAAILASHYAARQVTRLPLAQVLASAH"
     gene            complement(2875..3537)
                     /locus_tag="PLL14_03000"
     CDS             complement(2875..3537)
                     /locus_tag="PLL14_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02919.1"
                     /translation="MLTVTDLAKRHAGASRPLFEALEFAVAAGEVVALVGESGVGKST
                     LLNCIAGLETPDRGAVRIGPQALDMVRLDEAARAALRARRIGFVFQAFHVLPTLTVSQ
                     NLAVPLLLCDVPRGKHPQRIRSLLDRVGLADCAARWPTSLSGGELQRVAIARALVHQP
                     ALILADEPTGNLDPRTAGEVLSLLLDSVRERDTSALIVTHSQHVAQCCDRILTLTPAG
                     LQ"
     gene            complement(3566..4882)
                     /locus_tag="PLL14_03005"
     CDS             complement(3566..4882)
                     /locus_tag="PLL14_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOG02920.1"
                     /translation="MTGDVSAAPDAATPNAGEICIDASQLRPGVHVRLPVPWMDHQFM
                     FNSFVIADEEQARLIAAMKLPQLFCDPARCKVAPLPQRAADAPAGSDGEAEKAQLAAL
                     TAARMAEKRERARVMSELRGRLDKAQEHYISAAREVGGAIRSFSVNPRAAVERIAMVS
                     GNTTAALLADPDSAIVLIAEKAHDDGHAAHSLSVMTLALLLGKQAKLPEQALRALGIG
                     ALLHDIGKLAIRASILRSSERNRHEEVIYQSHCRAGHAEALRAGNLSPPLLDAILHHH
                     ERFDGGGFPDRLSGSAIPLSARLVAIANRFDNLVNPPDTRRAMSPSEALSTLWTREQK
                     AFDATLLQLFVRAMGVYPPGSIVQLSDGRIGAVVGSAPTGQPLSPKVMIYAPEVPRRQ
                     SIIIDLASEGVVKIDRPLRLQERPAEELDYLLPRRKINWSYMAEQK"
     gene            complement(4879..>5793)
                     /locus_tag="PLL14_03010"
     CDS             complement(4879..>5793)
                     /locus_tag="PLL14_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG02921.1"
                     /translation="GAARTVSRAIRGFATNPRQSVADLAAVCEKSTAALLADPDTAFV
                     LIADKGHDDGHAAHALSVMTLSLLLGKQAQLPEQALGVLGTAALLHDIGKRLISPSIL
                     RNSERNRHEEAIFRTHCRSGHDEAMRAGSLSKPMLDAILYHHERIDGSGFPERLSSNA
                     IPLAARIVAIADRFDTLVNPIDYRRALGPAEALSTMWTREQRGFDTSFLQLFVRAMGI
                     YPPGSIVQLSDGRIGAVVGSAPVGKPLLPKVMIYAPEVPHREAIIADLATQEVVTIER
                     VLRLQDRSVEEVRYLLPQRKVNWSCPAQ"
BASE COUNT          980 a         1861 c         2035 g          917 t
ORIGIN      
        1 aggttggcgc gggcggcgcg ttcggtatgc cgcagtcttt cgcctgggac ggtcagcgcg
       61 gcgtgcgccc agaggatctg gcgcgcggca agcggcgagc gcagtgtttc ggccatgccc
      121 ggccggttgc ggaagaaggt caactgaaag ccgatgtccg gctgcggcga gtccagtgcg
      181 ccggtgacat accaccattc ggtacgaaaa tccgggtggc caccgtggtc gcgcggaaag
      241 gcgagccccg gcccgggggt caccggggga taagcgagca acgcctcggc aggccaggtc
      301 agcgtcggca gcagcaaggg agtggcaagg aagcgacggc gttgcatggg ttcggtcggt
      361 gtgctcagtg ggcactggcc agcacctgcg ccagcggcag tcgggtgacc tggcgcgcgg
      421 cgtagtggct ggccaggatg gctgccgcca gggtcagccc ggcgccggcg aggagggtgg
      481 gccaggggat agagagtggc atgcgccagt gaaaggactg cggattgatc acatgcgtga
      541 ggatggcgcc gatcgccacg ccgcaggcga gaccgaccag caggccgacg caggcaatca
      601 gcgcaccttc gagcatcacc gcctggctca gcatccggcg gtcgaagccc agagcaccga
      661 gcgtagccag ttcgcgggcg cgcagccaga cgctggcggc gagtgtgacg gccagtccga
      721 acaggccgat cagcatcgcg gcggcttcga gcgcgtaagt gacggcaaag ctcttgtcga
      781 agatcgacag gctcaaacgg cgaatctcag ccgcttcgga aagttccagt ccgtgcctgc
      841 cggcgtacgg cgcgagccag gtggcggcag cggcttcctg gccggtccgg ggccacaagg
      901 cgaggtcggt aggctggaag cggccgccga ggtgctcata gtcctcacgg ctgatgacca
      961 aggcgccaaa ctgcctggaa tagtcgcgcc agacgccgcc gacgaagacc tccacctcgc
     1021 gctccagcaa gggcaggtgc aggatctgcc ccggcacgat ccggtagatc tcgtgcagcg
     1081 cttcgctgat ccagactacc gccatgtctg gcggtgccgc caccaactcg ccgctgaagg
     1141 gcaggcgtgc cgccgggttg ccgcgctcga tttcgcgcac cagtagcgcg acgtttggcc
     1201 gctgcgcatc gatggtcagc gaaccctgcg ccatgcgctc gactctggcg aacggcgtgt
     1261 ccgcctgcag caaggccgtg agcacttcag ccggcagcgg ggttgcccgg ctgcgcagat
     1321 agagcggtgc cggcaggatg tcgtcgagcc agcgatcgac ggcaacccga aagctggaaa
     1381 ccatgatcac catgctggcg gtcagtgcga aactgacgat caacccactg gcggccacct
     1441 gtgccagtag cggtgcctgg gcgacgttct gcagcgcgat ctgctgcggc gctggcagtg
     1501 ctctcccggc aagatggccg aagacctgcc gtgtcagcgg cggagcgctg gcgatgccga
     1561 tcagcaggac caggccaatc gccgcataac cgcccagcgg caggtcgaac cacggcggcg
     1621 cttgcgtcaa ggcgagcgca ccgatggcca gcagcgccgg cagcagtggc tggctggtgg
     1681 cggtcgggtc gctgccgcgc gggcgctgcg cgaaaccggt ttgcagtgct tgcgccagcg
     1741 cctgctggcg attcaggtac gccgggtaag acgcgccggc caggctggcg gcgcagccca
     1801 gcgccaggaa acccagggca ggcaccggag ccgggacgat gatcggtgcg ctgcccgtga
     1861 aatagccgcc gccaagatcg ccgcccagga cttgtgtgaa ggcgagggcc agcagatagc
     1921 cgagtcccag tccaagcagc gcgcccggta cgccgatggc gagcccttcg agcagtacct
     1981 gcaacaggcg catgcgtggc gacaggccga gaacgccgag cagggcaaac tgcgtcgaac
     2041 gctgtgcgac cgcgctcagt tgcgtggcga agaccagaaa ggcgccggtc agcaaggcga
     2101 ccagcgccag gacgttgagg ttcacacggt aggcgcgcga caggttggag acgcgtacgc
     2161 cgtcgtcgtc ggccatcctg agcagcaggc cgctcggcag tcgttgctcc agcgccgtac
     2221 gccaggctgc cgggtcggta tccggcccca ggcgtatgcg accctcgctg accgcgttgg
     2281 ccggcccgaa gtgttcctgg acccaggcga tgtcggcgac cagcagcagg tcgtcgggtc
     2341 ctgttgctgg cagatcgccg gcgatgatcg cgtgccagtc ctgatcacca cgctgcaagg
     2401 tcacggcgct gcccggatgc agcccgaatt tttcgagcag cgccggtgag gcatagacgc
     2461 ccccggcgag aatgtgcgcc gagccggcac gaccgtcatg tcgcggcagc agcctgggca
     2521 tcagcgccgc ggcgctgaac acgtcgatgc cgatcaggcg cagtggcgtc ggcgaactgt
     2581 cgaggcgtac gcggctttcg atgaccgctg cgaccagcag caccgcgggg tcgcgagcga
     2641 tttcgttgat gctcgcgagc gggacgctgc cggcgctgtc gcgggcggca agcacggcgt
     2701 cggggtttcc ctgcacggtc tgcattgctc gcgaaaaatc ggccagcgcg gcgtcgttga
     2761 tcagatggat ggcgtagccg agtgcaaccc cgagcgcgat cgccagcatc gacaacagcg
     2821 ttgcggcacg tcgcgtcctg aactgggcca ggatcaacca ccagctcagc ctgttcattg
     2881 caggcccgca ggcgtcaaag taaggatccg gtcgcagcac tgcgcgacgt gctgcgaatg
     2941 ggtgacgatc agcgcactgg tatcccgttc acgaacgctg tcgagcagca gcgagagcac
     3001 ctcgccggcg gtgcgcggat ccaggttgcc cgtcggttcg tcggccagaa tcagtgccgg
     3061 ttgatgtacc agcgcgcgcg cgatcgccac ccgttgcagt tcgccacccg acagcgacgt
     3121 cggccagcgt gccgcgcagt ccgccagacc gacacggtcg agcaggctcc ggatgcgctg
     3181 cgggtgtttg ccgcgtggca cgtcgcacaa caacaagggc acagcaaggt tctgcgacac
     3241 ggtcagcgtt ggcagcacat gaaaggcctg gaagacgaag ccgatacggc gcgctcgcaa
     3301 cgccgcgcgt gccgcttcat cgagccggac catgtcgagc gcctgcggac cgatccggac
     3361 cgcgccgcgg tcaggggttt cgaggccggc gatgcagttc agcagggtgc tcttgccaac
     3421 gccggattcg ccgacgaggg caacgacttc gccggccgcg acggcgaact ccagggcctc
     3481 gaacagcggt cggctggccc cggcatggcg ctttgcgagg tcggtgacgg tcagcatggc
     3541 gcagggccgg tgtccgggcc gcggatcact tctgttcggc catgtaggac cagttgatct
     3601 tgcggcgcgg cagcaggtag tccagttcct cggcgggacg ttcctgcagc cgcaatggcc
     3661 gatcgatctt gaccacgcct tcgctcgcca gatcgataat gatcgactgg cgacgcggga
     3721 cctccggcgc gtagatcatc accttcggcg agagtggctg gccggtgggc gcagatccga
     3781 ccaccgcccc gatccgacca tcgctgagct ggacgatcga gccgggtgga tagacgccca
     3841 tcgcgcgcac gaacaactgc agcagggttg cgtcgaatgc cttctgctcg cgtgtccaca
     3901 gcgtcgacaa ggcctcggaa ggcgacatcg cacgccgcgt gtcgggcggg ttgacgaggt
     3961 tgtcgaagcg gttggcgatg gccaccagcc tggccgacag gggaatcgca ctgccgctca
     4021 ggcggtccgg gaaaccgccg ccgtcgaagc gctcgtgatg gtgcaggatg gcgtccagca
     4081 agggaggcga caggttgccg gcgcgcaagg cctcggcatg gccggcgcgg cagtgtgatt
     4141 gatagatcac ttcctcgtgc cggttgcgct cgctgctgcg caggatcgac gcgcggatcg
     4201 ccaacttgcc gatgtcgtgc agcagggcgc cgattccgag ggcgcgcagg gcctgctcgg
     4261 gcagttttgc ctgttttccg agcagcagcg ccagggtcat gaccgacagc gaatgcgccg
     4321 cgtgcccgtc atcgtgcgcc ttttcggcaa tcagcacgat cgcgctgtca gggtcggcca
     4381 gcagtgctgc ggttgtgttc ccgctgacca tggcaatccg ttcgactgcc gcgcgggggt
     4441 tgaccgagaa actccggatg gcgccaccca cctcccgggc ggcgctgatg tagtgttcct
     4501 gtgccttgtc gaggcgtcct cggagttcgc tcatgacccg tgcacgctcg cgcttctcgg
     4561 ccatgcgtgc cgcggtcagg gctgccagtt gcgccttctc tgcttcccca tcactgccgg
     4621 caggggcgtc ggctgcgcgt tgcggcagcg gcgcaacctt gcagcgtgcc gggtcgcaga
     4681 acagttgagg cagtttcatg gccgcaatca gccgtgcctg ttcctcgtcc gcaatcacaa
     4741 aggagttgaa catgaactgg tggtccatcc acggcaccgg caagcgcacg tgcacgcctg
     4801 gacgcaactg cgaggcgtcg atgcagatct cacccgcgtt cggggtggcg gcatcggggg
     4861 ctgcggatac gtcgccagtc attgcgccgg gcaggaccag ttgaccttgc gttgcggcag
     4921 caggtatcgg acctcttcga cactgcggtc ctgcaggcgc agcacgcgct cgatggtcac
     4981 cacctcctgc gtcgccaggt cggcgatgat cgcctcgcgg tgtggcactt cgggcgcata
     5041 aatcatcacc ttcgggagca gcggcttgcc gaccggcgcc gaacccacca ccgcaccgat
     5101 ccggccatcg ctgagttgta cgatcgaccc gggcggatag atgcccatcg cgcgcacgaa
     5161 cagttgcaga aaactggtat cgaagccgcg ctgctcacgg gtccacatgg tcgacaaggc
     5221 ctcggcagga cccagcgcgc gacggtagtc gatgggattg accagcgtgt cgaagcggtc
     5281 ggcaatggcc actattcgcg ccgccagcgg tatcgcgttg ctgctgagtc gctccgggaa
     5341 tccgctgccg tcgatccgct cgtggtggta caggatggcg tcgagcatcg gtttcgacag
     5401 gctgccggcg cgcatcgcct cgtcgtgacc gctacggcag tgcgtccgga agatcgcttc
     5461 ctcgtgtcga ttgcgttcgc tgttgcgcag aatcgatggg ctgatcaagc gcttgccgat
     5521 gtcgtgcagc agcgcggccg taccgagtac gcccagtgcc tgctcgggca actgcgcttg
     5581 cttgccgagc agcagggaga gggtcatcac cgatagcgcg tgcgccgcgt gcccatcgtc
     5641 atgtcctttg tcggcaatca gcacgaaggc ggtatccggg tcggccagta gcgctgcagt
     5701 cgatttctcg cagacggcgg caaggtctgc tacggactga cgagggttgg tggcgaagcc
     5761 gcggatggct ctgctcaccg ttctcgcagc tcc
//
LOCUS       DAOSIT010000076         5791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12491_length_5791_cov_10.158473, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000076 DAOSIT010000000
VERSION     DAOSIT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12491_length_5791_cov_10.158473"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLL14_03015"
     CDS             complement(<1..947)
                     /locus_tag="PLL14_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG02922.1"
                     /translation="MLPDYPFATPPAAGETFEVSPGIHWLRMPLPFALNHINLWLLED
                     DAGWTIVDTGFALDMVKACWQQILARLPSTPRGGKIARIIVTHFHPDHLGLAAWLQEQ
                     TGAPVCMTLGEYLTAHAVWHEVGGHGNQPMLQQFRIHGLDADRCAALERRSGVYNRGV
                     PSLPTHYQRLFGGDRIAIGGHRWQVRIGFGHSPEHAALYCAELGVLISGDMLLPKIST
                     NVSVFAVTPLADSLGDYLQSIDRYRELPPETLVLPSHGLPFHGIENRVDALHAHHQER
                     LQVLEEHCNTPRSAAELLITLFPRDLDTHQTMFAMGEAIA"
     gene            1092..1574
                     /locus_tag="PLL14_03020"
     CDS             1092..1574
                     /locus_tag="PLL14_03020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOG02923.1"
                     /translation="MPFKLHTAAMHKAQDPHFSERVRASFARQEAMMLIGAVMPVVEP
                     GYTEIHLPFKPEITQQHGYVHGGVVGMIADSAAGYAASTLTTAETEVLTVEYKLNLLA
                     PADGQLLIAEGSVVRYGRTLIVTRAEVFAVKNGKKTPCALLQQTIMSIHGKKQPAVKT
                     "
     gene            1591..2754
                     /locus_tag="PLL14_03025"
     CDS             1591..2754
                     /locus_tag="PLL14_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HOG02924.1"
                     /translation="MEYPNLGFAHGETIDMLRASVRAFAADEIAPRAEAIDRDNLFPA
                     DLWRKLGGMGLLGLTAEEEYGGIGLGYLAHIVAMEEISRASASVALSYGAHSNLCVNQ
                     IRRNGTAAQKDRYLPKLISGEHVGALAMSEANAGSDVVSMKLRAERRGERFVLNGGKM
                     WITNGGDADTLVVYAKTDIDAGPRGITAFIVEKGMPGFSCGAKLDKLGMRGSNTYPLF
                     FDDCEVPAENVLGDVAGGVRVLMSGLDYERAVLAGGPLGIMAAAMDLVLPYVHDRQQF
                     GQPIGEFQLMQGKLADMYTTFNVCRAYVYAVGQACDRGETTRKDAAGAILYAAEKATW
                     MAGEAIQTLGGNGYINDYPAGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETL"
     gene            2762..3904
                     /gene="can"
                     /locus_tag="PLL14_03030"
     CDS             2762..3904
                     /gene="can"
                     /locus_tag="PLL14_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOG02925.1"
                     /translation="MTNTLRALTAEDTTALEHVRQFFRNYAAWLGVDLSFQNFEEEMA
                     SLPGAYALPSGRLFYAERNGQPAGCVGIRPFSAGLCEMKRLYVEPGQRGFEVGRDLAL
                     AAIKAAKAIGYRKLLLDTLPAMRVAVKLYRELGFKETPAYYQTPVEGTMFLALDLENW
                     SEEEVANENLFHLFDFNRAWSSRMQQVDPEYFVKLSRLQTPAYLWIGCSDSRVPANEI
                     VGLLPGEVFVHRNVANVVVHTDLNCLSVIQFAIDVLKVRHIMVVGHYGCGGVRAALNR
                     DRAGLVDIWLRHVRDVHDKHLALVDALPPERQHDRLCELNVLEQVANVCQTFVVQDAW
                     QRGQPVTVHGWIYGLKDGLMRDLGFTVHHGDDLLPGYRMALEALPT"
     gene            3930..4910
                     /locus_tag="PLL14_03035"
     CDS             3930..4910
                     /locus_tag="PLL14_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG02926.1"
                     /translation="MDIHHRGGATIALPPGVRSVPASPLTYLKLVATALIWGATWIAA
                     RVAVSEASPLAVASWRFLLAALVLGTLVVVHEGRPRWSHKEWFTLAALGASGIFLYNL
                     CFLYGMTMIEAGRGALVVALTPALVAASDWLLFGTPMSPTKAAGIVLAMFGCLLVVTN
                     GDPRLLFTGQVGLGEWLIIGCSALWAVYTFVGRRGTRSLSPLAMTFGASLTGWIMLTV
                     AALLQGSLFLLAETTWRGWSAIVFLGLFGTALAFTWYAEAVQRIGATRSAAFINLVPV
                     SAVILGAVLLDERLGVAVLSGGALVIAGVIITNHAGARLAAGVISKEKVA"
     gene            4907..>5791
                     /locus_tag="PLL14_03040"
     CDS             4907..>5791
                     /locus_tag="PLL14_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG02927.1"
                     /translation="MILTQEQEMIRDSLRDFAQERLAPFAAEWDRQHTFPAEALRELG
                     ELGALGMVVPEEWGGAGMDYMSLVLVIEEIAAGDGATSTIVSVQNSLACGITMKYGSD
                     EQKETWLKPLARGEMLGCFCLTEPHTGSDAAAISTRADRDGDHFVLNGVKQFITTGKY
                     ARVAIVFAVTDKAAGKKGISCFLVPTETPGYIVARIEEKMGQRASDTAQILFENCRVP
                     ASCLLGKEGDGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVQYAKERETFGKPIIEH
                     QAVSFRLADMATQIDAARL"
BASE COUNT         1040 a         1869 c         1851 g         1031 t
ORIGIN      
        1 gcaatcgctt cgcccatggc gaacatcgtc tgatgcgtgt cgagatccct ggggaacagg
       61 gtgatcagca actcggccgc gctgcgcggc gtgttgcagt gctcttcgag gacctgcagg
      121 cgctcctgat ggtgggcatg cagggcatcg acccggttct cgatgccgtg gaatggcaag
      181 ccgtgcgagg gcaggaccag ggtttccggg ggcagttcgc gataacggtc gatcgactgc
      241 agatagtcgc cgagcgagtc ggccagcggc gtaacggcaa agacgctgac attggtcgag
      301 atcttcggca gcaacatgtc gccggaaatc agcaccccga gctcggcgca gtacagcgcg
      361 gcgtgttccg gcgagtggcc gaagccgatg cggacttgcc agcgatgccc gccaatggct
      421 atccggtcgc cgccaaacag gcgctggtag tgcgtcggca aactcggtac gccacggttg
      481 tagacaccgc tgcgccgctc cagcgcggcg cagcggtcgg catcgagccc gtgtatgcgg
      541 aactgctgca gcatcggctg attgccgtgc cctccgactt cgtgccagac cgcatgcgca
      601 gtcagatact cgcccagcgt catgcacact ggtgcccccg tctgctcctg caaccaggcg
      661 gcaaggccga gatgatcggg gtggaaatgc gtcacgatga tgcgcgcgat ctttccgcca
      721 cgcggggtcg aaggcagtcg ggcgaggatt tgctgccagc acgccttgac catatcgagg
      781 gcgaaaccgg tatcgacgat ggtccagccg gcatcatcct cgagcaacca gagattgatg
      841 tggttcaacg cgaagggcaa cggcatccgc aaccagtgga tacccgggga aacctcaaag
      901 gtttccccgg cagcgggtgg cgtggcgaaa gggtaatctg gcagcatctg ggggcggatt
      961 gtaaaaagct ctgaagggta taaactgcgc gcctcagggg cctgcttgcg taggctccga
     1021 gtgcgcacca ttctacacgc gatccggctg cgagcagccc tgcgttggaa cggtcaggca
     1081 gcaacgtcca gatgcccttc aaactccaca cggcggccat gcacaaagcc caggacccac
     1141 atttttccga acgcgtccgc gccagttttg cgcgccagga agcaatgatg ctgatcggcg
     1201 cggtaatgcc ggtggtcgag ccaggctaca ccgagatcca cctgccgttc aagcccgaga
     1261 tcacgcagca acacggctac gtccatggcg gtgtggtcgg catgattgcc gattcggcag
     1321 ccggatacgc cgcgagtaca ctgaccaccg ccgaaaccga ggtgctgacc gtcgagtaca
     1381 agctcaacct gctggctcct gccgacggac agttgctgat tgccgaggga tcggtagttc
     1441 gctacgggcg cacgctgatc gtcacccgcg ccgaggtgtt tgccgtcaag aacggcaaaa
     1501 agaccccttg cgcgctgctg cagcagacga tcatgtcgat acacggcaaa aagcaaccag
     1561 ccgtcaaaac ctgatcaagg agcgagagac atggaatacc cgaacctcgg ttttgcccat
     1621 ggcgaaacca tcgacatgct gcgcgccagc gtgcgtgcct ttgccgccga cgaaattgcg
     1681 ccgcgtgccg aagccatcga tcgcgacaac ctgtttcccg ccgacctgtg gcgcaagctg
     1741 ggtggcatgg gcctgctcgg ccttaccgcc gaggaagagt acggcggcat tgggctgggc
     1801 tatctcgcgc acatcgtcgc catggaggag atttcccgtg cctcggcgag tgtcgcacta
     1861 tcctacggcg cgcactccaa cctctgcgtc aatcagattc gtcgcaacgg cacggcggcg
     1921 cagaaggatc gctacctgcc gaagctgatt tccggcgaac acgtcggcgc actggccatg
     1981 tcggaagcca acgccggctc ggacgtggtg agcatgaaac tgcgcgccga gcgccggggc
     2041 gagcgcttcg tcctcaacgg tggcaagatg tggatcacca acgggggtga cgccgatacc
     2101 ctggtggtct atgccaagac cgacatcgac gccgggccgc gcggcatcac cgccttcatc
     2161 gtcgaaaagg gcatgcccgg cttctcctgc ggcgccaagc tcgacaagct cggcatgcgc
     2221 ggctcgaaca cctacccgct gttcttcgat gactgcgagg tccccgcaga aaacgtgctc
     2281 ggcgacgtcg ctggcggagt gcgggtgctg atgagcggtc tcgactacga gcgcgccgtg
     2341 ctcgccggcg gcccgctggg aatcatggcg gcggccatgg atctggtact cccctacgta
     2401 catgaccgcc agcagttcgg ccagccgatc ggtgaatttc aattgatgca gggcaagctc
     2461 gccgacatgt ataccacctt caacgtctgc cgggcctacg tctatgccgt cggccaggcc
     2521 tgcgatcgcg gtgaaacgac gcgcaaggac gccgccggag cgatcctcta tgccgccgaa
     2581 aaagcgacct ggatggccgg cgaggcgatc cagacgctcg gcggcaacgg ctacatcaac
     2641 gattatccgg ccgggcggct gtggcgcgac gccaagctct acgagatcgg cgccggcact
     2701 tccgaaatcc gacgcatgct gattggtcgc gaactcttcg ccgagacgct gtgagccggc
     2761 tatgaccaac acgctgcgcg cgctcaccgc cgaagatacg accgccctcg aacacgttcg
     2821 ccagttcttt cgcaattacg cagcctggct cggcgtcgac ctgtcgttcc agaatttcga
     2881 agaggagatg gcttcgctgc cgggagccta tgcactaccc tccggccgtc tgttctatgc
     2941 cgaacgcaac ggtcagccgg caggctgcgt cggtattcga ccgttttcgg ccggactctg
     3001 cgaaatgaag cggctgtatg tggaacctgg ccagcgcggt ttcgaagtgg gccgcgatct
     3061 ggcgctggcg gcgatcaagg cggccaaggc catcggctac cgcaagctgc tgctcgacac
     3121 actgccggcc atgcgcgtcg ccgtcaagct ctatcgcgaa ctgggtttca aggagacacc
     3181 ggcgtactac cagacgccgg tcgagggcac gatgttcctg gcgctcgatc tggagaactg
     3241 gtccgaggaa gaagtcgcca acgaaaacct gttccacctc ttcgacttca accgcgcctg
     3301 gtcgagccgc atgcagcagg tcgatcccga atatttcgtc aaactgtcgc ggctgcagac
     3361 gccggcctac ctgtggatcg gctgctccga ttcgcgcgtg ccggcgaacg agattgtcgg
     3421 cctgctgccg ggtgaggtct tcgtccatcg caacgtcgcc aatgtcgtcg tgcataccga
     3481 cctcaactgc ctgtcggtga ttcagttcgc catcgatgtg ctcaaggtca ggcacatcat
     3541 ggtcgtcggc cattacggtt gcggcggtgt acgggccgcc ctcaatcgtg accgtgccgg
     3601 actggtcgac atctggctgc gacatgtgcg cgacgtgcac gacaagcatc tggcgctggt
     3661 cgatgccctg ccgcctgagc ggcagcatga tcggctctgc gaactcaacg tgctcgaaca
     3721 ggtggccaac gtgtgccaga ccttcgtcgt ccaggacgcc tggcaacgcg gtcagccggt
     3781 taccgtgcat ggctggatct atggactgaa ggacgggctg atgcgcgacc tcgggttcac
     3841 cgtacaccac ggcgatgacc tgctgcccgg ctaccgcatg gcgctggagg cgctgccgac
     3901 ttgagcaccg ctgtccttgc gcgacgccga tggacatcca ccaccgcggt ggtgcgacca
     3961 ttgccctgcc cccgggggtc cgaagcgtgc ccgcatcacc actgacctat ctgaagctgg
     4021 tcgccaccgc cctgatctgg ggggcgacgt ggatcgccgc ccgcgtggcg gtcagcgagg
     4081 cgtcgccgct ggccgtcgcc agttggcgtt tcctgctcgc ggcattggtc ctggggacgc
     4141 tggtggtggt ccatgagggg cgcccgcgct ggtcgcacaa ggaatggttc accctcgccg
     4201 cactcggtgc cagcggcatc ttcctctaca acctgtgttt cctttacggg atgacgatga
     4261 tcgaagccgg ccgtggcgcg ctggtggtcg ctctgacgcc ggcgctggtc gccgccagcg
     4321 actggctgct gttcggcacg ccgatgtcac cgaccaaggc ggcgggaatc gtactggcga
     4381 tgttcggctg ccttctggtg gtcaccaatg gcgacccacg actgctgttc accggtcagg
     4441 tgggcctcgg cgaatggttg atcatcggct gcagtgcgct gtgggcggtt tataccttcg
     4501 tcggtcggcg tggcacgcgc tcgctgtcgc cgctggcgat gaccttcggc gccagcctca
     4561 ccggctggat catgctcacc gtcgcggcgc tgctgcaggg ttcgctgttt ctgctggcgg
     4621 agacgacctg gcgcggctgg agcgccatcg tattcctcgg gctgttcggc acggcactgg
     4681 ccttcacctg gtacgccgag gccgtgcagc gcatcggcgc cacgcgttcg gcggcattca
     4741 tcaacctggt tccggtcagc gccgtcatcc tcggtgcggt gctgctcgac gaacgtctcg
     4801 gggtcgccgt tctgtccggc ggggcgctgg tcatcgccgg cgtcatcatc accaatcacg
     4861 ccggcgcgcg ccttgcggcc ggcgtcatca gcaaggagaa agtggcatga tcttgacaca
     4921 ggaacaggaa atgatccgcg actcgctgcg cgatttcgcg caggagcgcc tggccccctt
     4981 tgccgccgaa tgggaccgcc agcacacctt tcccgccgag gcgctgcgcg aactcggcga
     5041 gctcggcgca ctcggcatgg tcgtacccga agagtggggc ggtgccggca tggactacat
     5101 gtcgctggtg ctggtcatcg aggaaatcgc tgccggtgac ggggcgacct cgaccatcgt
     5161 cagcgtgcag aactccctgg cctgcggcat taccatgaaa tatggcagcg acgaacagaa
     5221 ggaaacctgg ctcaaaccac tggcgcgcgg cgagatgctc ggttgttttt gcctcaccga
     5281 gccgcatacc ggttccgatg cagcggcgat cagcacccgc gccgaccgcg atggcgacca
     5341 tttcgtgctc aacggcgtca agcagttcat caccaccggc aagtacgccc gggtagcgat
     5401 cgtcttcgcc gtcaccgaca aggctgccgg caaaaagggc atttcctgct tcctggtccc
     5461 gaccgagacg ccgggctaca tagtcgcccg catcgaggaa aaaatgggcc agcgcgcttc
     5521 cgacaccgcg cagatccttt tcgagaactg ccgggtgccg gcgtcgtgcc tgctcggcaa
     5581 ggaaggtgac ggttaccgca tcgcgctgtc aaacctcgaa gccggccgca tcggcatcgc
     5641 cgcccagtcg gtcggcatgg cgcgtgccgc cttcgaagcc gcggtgcaat acgccaagga
     5701 gcgcgaaacc ttcggcaaac cgatcatcga gcatcaggcg gtcagcttcc gcctcgccga
     5761 catggccacc cagatcgatg ccgcccgcct g
//
LOCUS       DAOSIT010000077         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12695_length_5717_cov_9.219887, whole genome shotgun sequence.
ACCESSION   DAOSIT010000077 DAOSIT010000000
VERSION     DAOSIT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12695_length_5717_cov_9.219887"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLL14_03045"
     CDS             complement(<1..275)
                     /locus_tag="PLL14_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HOG02928.1"
                     /translation="MAQVLNLTASGPLNNASGNPHLDEWRSFLEGCRQVTSQVSSLLP
                     SGHLTRINGLVMEASGLKLPLGSSCRIMPVGGSAIEAEVVGFNGERL"
     gene            complement(260..961)
                     /locus_tag="PLL14_03050"
     CDS             complement(260..961)
                     /locus_tag="PLL14_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOG02929.1"
                     /translation="MSHWIPKEKMTAYQRWEVAAFDEEQRTAVNPPPPAEPPVMEQPI
                     DLPVEPASVPQEQEPPVVLPTAEDIERMHQEAHAAGHAAGYAEGMVAGKTAAARIAAL
                     MDSLQQALAAIDQGVADQLLALAIEIANQVLRQSLRVQPELLLPVVREAVTVLHPHHG
                     QPLLFVHPDDAALVRSQLGDQLAHSNWRIVEDKALSAGGCRVELGASEVDATLETRWR
                     RVTESIGASQEWLKS"
     gene            complement(975..1973)
                     /gene="fliG"
                     /locus_tag="PLL14_03055"
     CDS             complement(975..1973)
                     /gene="fliG"
                     /locus_tag="PLL14_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOG02930.1"
                     /translation="MPADDGVEKSAILLIALGEDYAAEVLKHLGPKEVQKLGHAMAAL
                     KSVPRARVEEVLSEFHKTAEESAAVNIDTDAYVRSVLTKALGDDKASNLISRILQPGE
                     TNGIEGLKWMDAPTVADLIKNEHPQIIATILVHLAHDHSSEILNQFSERLRNDVVLRV
                     ATLEGIQPEALKELNDVMLRLLSGSSSIKKSAMGGVRTVAEMLNFMGTANETAVLDSI
                     REYDPDLAQKILDEMFVFENLLDLEDRAIQLLLREIQSESLILAMKGATEPLREKIFR
                     NMSQRASEMLREDLDSRGPVRLSEVESEQKEILKIVRRLADEGQITLGGAGGEEMV"
     gene            complement(1963..3696)
                     /gene="fliF"
                     /locus_tag="PLL14_03060"
     CDS             complement(1963..3696)
                     /gene="fliF"
                     /locus_tag="PLL14_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOG02931.1"
                     /translation="MAAAGTETTGTTGAGNPLDRAREALARLSNRQKVVLMVSIAAVI
                     ALLVAAATLLRQSEFRILFSNITERDGGAIIASLEQMNIPFKFNDSGSAILVPSNRVH
                     DVRLRLASQGLPRGGAVGFELMESQKFGISQFAEQVNYQRGLEGELSRTIQSVAAVQA
                     ARVHLAIPKPSVFMREELKPSASVMLNLYPGRSLDAAQIAGIQNLVAASVPQLAASSV
                     TLLDQSGTLLSQMKSKLLEAGLDPTQVKYIQEVEASIIKRVEDILAPIVGPGNARVQI
                     AADIDFSQSEQTAESHRPNTTPPEISIRSQQTSETASTNPTAQGVPGALSNQPPVPAT
                     APLTQPAVPGAGAAATPAQPLPGQLNAAGVQAPIASVGQPISTSKNSTINYELDKTIR
                     HVKQSVGTVKRLSGAVVVNQRKEIGKDGKAVNKPLPDNELKQISDLVKEAMGYSKERG
                     DTISVANAPFTVVEKDEGPPLWRDPETVSLAKELIKYGVIAAIIAYLLLGVVRPLVKT
                     MLPSPPESEASVGGNIDLVAGEEEDEAKEHVPTAAELFDKKLAVARALAQQDPHIVAN
                     IIKEWTGANAS"
     gene            3820..4980
                     /locus_tag="PLL14_03065"
     CDS             3820..4980
                     /locus_tag="PLL14_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG02932.1"
                     /translation="MPSPELKAQELQRAFDVFNQVSLALTQSYQGLQSRVESLTAELA
                     VANGELRRQYEEKQALSERLSLLLNALPAGVVVLDSAGRVSEANPAARQMFAQPVITE
                     SWPALMQARLLATEAPGEWQLDARRLAIVESPLDSAGGRILLIHDVTAAHGLKADLER
                     HQRLAAMGEMAASLAHQLRTPLATALLYSANLGQPQLADDARRRFADKATAQLKRLER
                     LIQEVLLFARGESIGRDSIRADELLADAAQTVQPLFIERNVNFRVVCQTGDAVVTGSR
                     KALAGALVNLLENALQACSSSGEVVLGATRVSQQLRIGVRDTGSGIAPEVQGRVFDPF
                     FTTRGQGTGLGLAIALGVARAHGGTIEVSSVPGEGSEFVIVLPADTTKERNH"
     gene            4996..>5717
                     /locus_tag="PLL14_03070"
     CDS             4996..>5717
                     /locus_tag="PLL14_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG02933.1"
                     /translation="MAGGFPILIVEDDAALREAVCDTLELAGQAVVSAGGGEEALQLL
                     AKRAVSLVVSDVRMLPIDGIALLKQIRSRFPQLPVVLMTAFADVDRAVEAMRAGACDF
                     LLKPFEPKALLEHVLRYRLPEALDDDGVIAHDALTRNLFALAGRVAQSDTTVLLTGES
                     GVGKEVVARHIHLYSGRSKGPFVAINCAAIPDGLLEATLFGHEKGAFSGAQQAQAGKF
                     EQAQHGTLLLDEITEMPLGLQA"
BASE COUNT          995 a         1709 c         1800 g         1213 t
ORIGIN      
        1 aagagtcgtt ccccattgaa gcctaccact tcagcttcga tcgccgagcc accaaccggc
       61 atgattcggc aagaactgcc gagaggcaac tttagccccg aggcctccat gacgaggccg
      121 ttgatgcgcg tcaggtggcc gctcggaagc agcgaactga cctggctggt gacttgtcgg
      181 caaccttcga ggaagctgcg ccattcgtcg agatgtgggt ttccggaagc gttgttcaat
      241 ggaccgctcg ctgtgaggtt caggacttga gccattcctg gctggcgcca atgctttcag
      301 tgacacgtcg ccaacgtgtt tccagcgtcg cgtcgacttc gctcgcaccc agttccacgc
      361 ggcagccgcc tgcactcagt gccttgtctt cgacgattcg ccagttgctg tgcgcaagct
      421 gatcgcctag ctggctgcgt accaaagccg catcatccgg gtgcacgaac agcagcggct
      481 gaccatgatg ggggtgcagc accgtcaccg cttccctgac cactggcagg agcagttccg
      541 gttgcacgcg caaactttga cgcaacacct gattggcaat ctcgatcgcc agcgccagca
      601 actggtcggc gacgccctgg tcgatagccg cgagtgcctg ctgcaggctg tccatcaggg
      661 cggcgattct cgctgccgcg gtcttgcccg ccaccatacc ctcggcatag cctgcggcat
      721 ggccggcagc gtgcgcttcc tggtgcatgc gctcgatgtc ttcggcagtg ggtaggacaa
      781 ccggcggctc ctgctcctgc ggcacgctgg cgggctcgac cggcaggtcg atgggttgtt
      841 ccatgactgg cggttcggca ggcggcggag ggttgaccgc cgtccgctgc tcttcatcga
      901 aggccgccac ctcccagcgc tggtaggcgg tcattttttc cttgggaatc cagtggctca
      961 tgtggctttg atgctcaaac catttcctca ccacccgcgc cgccaagcgt gatctggcct
     1021 tcgtcagcca gacgacggac aatcttgagg atttccttct gctcggattc gacttccgag
     1081 agccgaaccg ggccacgcga atcgagatct tcgcgcaaca tttccgaagc cctttgcgac
     1141 atgttccgga agatcttctc gcgcaagggc tcggtggcac ccttcatggc caggatcagc
     1201 gactcggact gaatttcgcg caacagcagc tggattgccc ggtcttccag atcgaggaga
     1261 ttctcgaaga cgaacatctc gtcgaggatc ttttgcgcga ggtcaggatc gtattcgcgg
     1321 atcgagtcga ggaccgcggt ctcattcgcg gtgcccatga aattgagcat ctcggcgacc
     1381 gtccggacgc cacccatggc agacttcttg atgctcgatg aacccgatag caggcgtagc
     1441 atgacgtcgt tcaactcctt cagggcttcc ggctgaatgc cttcgagggt ggccacgcgc
     1501 aggacgacat cgttgcgcag acgttcggaa aactggttca ggatctcgct ggaatggtca
     1561 tgagccagat gcaccagaat ggtcgcaata atctgaggat gctcgttctt gatcaggtcg
     1621 gcgaccgtcg gcgcatccat ccatttgagg ccttcaattc cgttggtctc gcccggttgc
     1681 aggatccttg agatcaggtt ggatgccttg tcatctccga gggctttggt gaggaccgaa
     1741 cgaacatagg cgtcggtatc gatgtttact gcggcggact cctccgcggt cttgtggaac
     1801 tcgctcagta cctcttcgac acgcgcacgt ggtaccgatt tcaacgccgc catggcgtgc
     1861 ccgagcttct gcacttcctt cggccccagg tgtttgagga cttcagcggc gtagtcctcg
     1921 ccgagcgcga tcagtagaat ggcgctcttt tcgacgccgt cgtcagctgg cattggcgcc
     1981 ggtccattcc ttgatgatgt tggctacgat atgcgggtcc tgctgtgcca gggcgcgtgc
     2041 caccgccagt ttcttgtcga acagttcagc ggctgtcggg acatgttcct tcgcttcatc
     2101 ttcctcctcg ccggcgacga gatcgatatt gccgccgacg ctggcctcgc tctccggcgg
     2161 gctggggagc atcgtcttga ccagcggccg caccactccg agcagcagat aggcgatgat
     2221 ggcggcaatc acgccatact tgatcaactc tttggccaag gaaaccgttt ctggatctcg
     2281 ccacagcggc ggtccctcat ccttctcgac gacggtgaac ggcgcgttgg ccaccgaaat
     2341 ggtatcgccg cgctccttgc tgtagcccat ggcctccttg acgaggtcgc tgatctgttt
     2401 cagttcgttg tcgggcagcg gcttgttgac cgctttgccg tccttgccga tttccttgcg
     2461 ctgattgacc accaccgcac ccgacaggcg ctttaccgtg ccgaccgatt gcttgacgtg
     2521 gcggatggtc ttgtcgagtt cgtagttgat ggtcgaattc ttgctggtgc tgatcggttg
     2581 tccaacgctg gcgattggcg cttgcacgcc ggcggcgttg agctgtcctg gcagtggctg
     2641 ggccggtgtc gcggctgcac ctgcacccgg caccgccggt tgagtcagcg gggcggttgc
     2701 cggcactggg ggttgattgc tgagggcacc cggaacgcct tgtgcggtcg ggttggtgct
     2761 ggcggtttca ctggtttgct ggctacgaat gctgatctcg gggggcgtcg tgttcggtcg
     2821 atgcgactct gccgtttgct cggactgcga aaaatcgatg tctgcggcga tctggacacg
     2881 ggcattgccc gggccgacga tcggcgccag aatgtcctcc acgcgcttga tgatgctcgc
     2941 ctcgacttcc tggatgtatt tgacctgcgt cgggtcgagg ccagcttcga gcaacttgct
     3001 tttcatttgc gacagcaggg tgccgctctg atcgaggagg gtgacgctcg aagccgccaa
     3061 ctgtggcacg ctggcggcta cgagattctg gatgccggcg atctgggccg cgtccagcga
     3121 ccgtccagga tagagattga gcattacaga agccgatggc ttcagctctt cgcgcatgaa
     3181 gacactcggc ttgggtatcg ccaggtgtac ccgcgcggcc tgaacggcag ccaccgactg
     3241 aatggtgcgc gacaattcgc cctcgagacc gcgctgataa ttcacttgct cggcaaactg
     3301 gctgatgccg aatttctgac tttccatcaa ttcgaagccg actgctccac ccctcggcag
     3361 tccctgcgaa gcgagccgca gacgcacatc gtgaacccgg ttcgaaggaa cgagaatggc
     3421 gctgccggag tcgttgaact tgaacgggat gttcatctgt tccagagatg cgatgatggc
     3481 accgccgtcg cgctccgtaa tgttggagaa gagtatcctg aactcgcttt gtctgaggag
     3541 cgtggcggcc gcgacgagta gtgcgatcac cgcggcgatc gaaaccatca gcaccacctt
     3601 ctgccggttg ctcaatcgcg ccagagcctc gcgagcgcga tccagcggat tgcccgcgcc
     3661 tgttgtccct gtggtttcgg ttccggctgc cgccatgcct gcgatacggt gataaacctg
     3721 cgattaagca agcaaaaagt gaaaaagggc tgataatctc gctgctattg tagctgttaa
     3781 ttgcaccgga ccttcgatcg gatgactgaa gtcggacaaa tgccgagccc cgaactcaaa
     3841 gcgcaggaac tgcagcgcgc tttcgatgtg ttcaatcagg tttcgctggc gctgacacag
     3901 tcgtatcagg ggctacagag ccgggtcgag tcgctgacgg cggaattggc ggtcgccaat
     3961 ggtgaacttc gtcggcagta cgaggagaag caggcgctct cggaacgcct ttccttgttg
     4021 ctcaatgcct tgccggcggg agtggtcgtg ctcgacagcg caggccgggt cagcgaggcc
     4081 aatcctgccg cacgccagat gtttgcccag ccggtgatca ccgaatcctg gccggcattg
     4141 atgcaggcca gactgctggc gactgaggcc cctggcgagt ggcagctcga tgcgcggcgc
     4201 ctggccattg tcgagagccc gctagactcg gcaggtgggc ggattctact gattcacgat
     4261 gtgactgcgg cgcacggtct gaaggccgat ctcgaacgtc accagcgtct ggcggcgatg
     4321 ggcgagatgg cggcgtcgct ggcgcaccag ttgcggaccc cgctggcaac ggcattgctg
     4381 tacagcgcca atcttggtca accgcagctc gccgacgacg ctcgcaggcg ttttgcggac
     4441 aaggcgactg cccagttgaa gcgcctcgag cgcctgatcc aggaagtgct gctctttgcc
     4501 cgcggcgaga gcatcggccg tgactcgatt cgtgccgacg aactgcttgc cgatgccgcg
     4561 caaaccgtgc aaccattgtt catcgaaaga aatgtgaatt tccgggtagt ctgccagact
     4621 ggcgacgcag tcgtcaccgg cagccgtaag gccttggccg gcgccctggt gaatctgctc
     4681 gagaacgcat tgcaggcctg ctcatcgagt ggggaagtgg tgcttggcgc gacccgcgtc
     4741 agccaacagt tgcgcatcgg cgttcgcgat actggctccg gcattgcacc cgaggttcaa
     4801 ggacgtgtct ttgatccgtt cttcaccacg cgcggccagg gtaccgggct cggcctggcg
     4861 attgctctgg gggtggcgcg tgcgcacggg gggaccattg aggtcagttc ggtgccgggc
     4921 gaggggtcgg agttcgttat cgtgctgccc gccgacacca ccaaagagcg aaatcactga
     4981 ggaaggctgg aacggatggc gggcggattc ccgattctga ttgttgagga cgacgccgca
     5041 ctgcgtgagg cggtatgcga cacgcttgaa ctggccgggc aggcggtagt ctcggccggc
     5101 ggcggcgagg aggcgctgca attgctcgcc aagcgtgccg tgtcgctggt cgtcagcgat
     5161 gtgcgcatgc tcccgatcga cggcattgcc ttgctgaagc aaatccgcag ccgttttcca
     5221 cagcttccgg tagtcctgat gacagctttc gccgacgtcg accgggccgt cgaagcgatg
     5281 cgcgcaggcg cgtgtgattt tctcctcaag cccttcgagc cgaaggcact actcgaacac
     5341 gtgctgcgct atcgcctgcc ggaagcgctg gacgacgatg gcgtcattgc ccatgatgcg
     5401 ctgaccagga acctttttgc gcttgccggc cgggtcgcgc agagtgatac cacggtcttg
     5461 ctgaccggcg agagcggcgt cggcaaagag gtggtggccc gccatattca cctgtactcg
     5521 gggcgcagca aaggtccttt cgtggccatc aactgcgcgg ccatcccgga tggactgctt
     5581 gaggcgacgc ttttcggtca tgaaaagggt gcgttcagtg gtgcccagca ggcacaggct
     5641 ggaaagttcg agcaggcgca gcatggcacg ttgttgctcg acgagatcac cgaaatgccg
     5701 cttggcctgc aggccaa
//
LOCUS       DAOSIT010000078         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12707_length_5714_cov_9.963421, whole genome shotgun sequence.
ACCESSION   DAOSIT010000078 DAOSIT010000000
VERSION     DAOSIT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12707_length_5714_cov_9.963421"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="PLL14_03075"
     CDS             <1..1971
                     /locus_tag="PLL14_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOG02934.1"
                     /translation="IVALIVALVQGTLPLLGAHRGQQPWIVLARPAAVAQALLLAVAF
                     ACLIMAFIDNDFSVLYVAQHSNTLLPLEYRVAAVWGGHEGSLLLWVVMLALWTLAVAV
                     RSKQLPEHLVARVLGVLGLVSAGFLLFILVTSNPFERLLPGAEEGRDLNPLLQDPGLI
                     IHPPMLYMGYVGFSVAFAFAIAALLSGQLDTAWARWSRPWTTAAWIFLTMGIALGSWW
                     AYYELGWGGWWFWDPVENASFMPWLVGTALVHSLAVTEKRGSFKNWTVLLAISAFSLS
                     LLGTFLVRSGVLTSVHAFATDPRRGIFILLFLGTVIGSSLALFAWRAPRVGLGGRFGL
                     LSRESLLLTNNVLLVVACASVLLGTLYPLLIDALGAGKLSVGPPYFDAVFVPLMLPAA
                     FLIGVAPFARWKHASVSELAHTLRWAFLAAALVGIGTPFIYGQWKPLVALSLLLAAWI
                     VLSALLNFLERVQSTRAGQSFIAAALRQPRSFFGMHLAHVGIAVFIVGVTMVGAYQAE
                     KDVRMEVGDTVSVGNYTFRFNGVRPQQGPNYRALLGDIDLIKDGRVLRKMFPEKRFYV
                     ASAMPMTEAAIDSGLLGDVYVSLGEPIDKARPDAAWAVRVYHKPFVDWIWGGCVLMAF
                     GGLLAISDRRYRIKARAGSPASSAAASLVQNA"
     gene            1982..2566
                     /locus_tag="PLL14_03080"
     CDS             1982..2566
                     /locus_tag="PLL14_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOG02935.1"
                     /translation="MNRFLWPLIGFIVLVVLLAVGLNLNPRDVPSPLVGKPAPNFSLS
                     QLAAPEKKFSPQDMLGKVWLLNVWASWCVSCRQEHPVLVELAKKGTVPLVGLNYKEVR
                     GDGGIDLDKVAPGSEQSMVIARASTWLRTHGNPYTLSVLDIDGRVGIDYGVYGVPETY
                     VIDKAGIIRMKHTGPISPDVFSGKILPLLAELSR"
     gene            2563..3048
                     /locus_tag="PLL14_03085"
     CDS             2563..3048
                     /locus_tag="PLL14_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOG02936.1"
                     /translation="MKRLLLVVLLAMAGTGWHSLHAGEARPLAADEATEQRLIAIASE
                     LRCLVCQNESLSGSNAELANDLRREIRGLIRDGRSDAEIMEFMVSRYGDFVRYRPPLK
                     GSTLLLWFGPGLLFVTGLTSLVIYLRRRDRAIRNTPLTAAEQQRAEALLQDRDSGQGP
                     S"
     assembly_gap    3080..3179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3180..4016
                     /locus_tag="PLL14_03090"
     CDS             <3180..4016
                     /locus_tag="PLL14_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HOG02937.1"
                     /translation="QTRIAPEQILEMVNGLVEKLRKNPDDSQGWVMLARSYKVLERFP
                     EAADAYSRAATLVDQDPVLLADYADVLSRSNGGKLQGKPTELVERALKLDPDEPQALL
                     LAGAAATDRQEFAAAAEYWSRLHAQLEPDSEEARALAAAINKAREIATQAAAAKTAGK
                     PAAGVGAVSGEVTLSSRLAALARPDDVLFVFARAEEGPRMPLAAMRATVADLPLRFIF
                     DDSMALPGGRKISDFATISIEARVAKAGKAQSSSGDLSGSLSGIKPGSRNLRLQIDQV
                     QP"
     gene            4167..4757
                     /locus_tag="PLL14_03095"
     CDS             4167..4757
                     /locus_tag="PLL14_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOG02938.1"
                     /translation="MSDNVPGFWTKLKRPSTRYSLLALLSVGFVCGVLFWGGFNTAME
                     ATNTLDFCISCHEMRDNVYQEYKTTIHYSNRTGVRAYCSDCHVPKDWGHKMMRKIQAS
                     QELYGKIMGTISTPEKFEAKRLELATHEWARMKASDSRECRNCHSFSAMDIERQKARA
                     SKMHKLGQDDKKTCIDCHKGIAHRKPKNMPEEPDDD"
     gene            4867..5703
                     /locus_tag="PLL14_03100"
     CDS             4867..5703
                     /locus_tag="PLL14_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HOG02939.1"
                     /translation="MKRTSLSLAILGVVLTLGAPMAQAAPDWSKVPKRDVQVFHPAVT
                     PIEWVMKKSDHSGRTGLNKGESCVGCHEEKGGLNFDMKRLAGKDLEPKGAPKTMNFPV
                     SVQSAYDKDNAYIRLTFKAPSGAVDPADKENDVKVAVMFANDKVTQGAQIGCWATCHS
                     DARTMPGADEKKTKYTKDGAYELMQWKSAKGAKVADGSVTDKRNMEGGTAGVKAEGSK
                     VGETYTVTFTRKLSGTLAEGKATPFGVAIHSDHASGRFHHVSMGYTMGFGADGDIKST
                     KQ"
BASE COUNT         1040 a         1779 c         1742 g         1053 t
ORIGIN      
        1 atcgtcgcgc tcatcgtcgc cctggtacag ggtaccttgc cgctgctcgg cgcccaccgc
       61 ggccagcagc catggattgt gctcgcccgt ccggcggcgg tcgcgcaggc gctgctgctg
      121 gcagtggcct tcgcctgcct gatcatggcc ttcatcgaca atgacttttc ggtgctttat
      181 gtggcgcaac attcgaacac gctgctgccg ctcgagtacc gcgtcgctgc ggtctggggc
      241 ggacacgaag gctcgctgct gctgtgggta gtgatgctgg cgctgtggac gctggcggtt
      301 gccgtgcgct cgaagcaact ccccgaacac ctggtcgcgc gcgtgctcgg cgtgctcgga
      361 ctggtcagcg ccggcttcct gctgttcatc ctggtgacct cgaacccctt cgaacgactg
      421 ctgccaggcg ccgaagaggg gcgcgatctg aacccgctgc tgcaggatcc cgggctgatc
      481 attcacccgc cgatgttgta catgggctat gtcggcttct cggtagcctt cgccttcgcc
      541 atcgccgcgt tgctctccgg acagctcgac accgcctggg cgcgctggtc gcgcccctgg
      601 accacggcgg catggatctt cctgaccatg ggtatcgcgc tcggatcgtg gtgggcctat
      661 tatgaactcg gctggggcgg ctggtggttc tgggacccgg tcgaaaatgc ttcgttcatg
      721 ccctggctgg tcggcacggc gctggtgcat tcgctggcgg tcaccgaaaa gcgcggcagt
      781 ttcaagaact ggacggtact gctggccatt tcggcgtttt cgctgtcgct gctcggaacc
      841 ttcctggtcc gttccggtgt gctgacttcg gtgcacgcct tcgccaccga tccgcggcga
      901 ggcatcttca ttctcctttt cctgggaacg gtgatcggca gttcattggc cctgtttgcc
      961 tggcgcgcgc ccagagttgg tcttggcggc cgctttggcc tgctttcgcg cgagtccttg
     1021 ctgctgacca acaacgtgct gctggttgtc gcctgcgcgt cagtattact gggcacgctc
     1081 tatccgctac tgatcgatgc tctcggcgcc ggcaagctgt cggtaggtcc gccctacttc
     1141 gacgccgtct ttgtcccgct gatgttgccg gcggcttttc tgatcggtgt ggcacctttt
     1201 gcacgctgga agcatgccag cgtcagcgaa ctcgcacaca cgctgcgctg ggcatttctg
     1261 gccgccgccc tggtaggcat cggtaccccg ttcatttatg gtcagtggaa accactggtg
     1321 gcattgagcc tgttgctggc agcatggatc gttctcagcg cgctgctgaa tttcctcgaa
     1381 cgcgtgcaat ccacgcgtgc agggcagtcg ttcatcgctg cggcgttgcg gcaaccgcga
     1441 agttttttcg gcatgcacct ggcgcatgtg ggtatcgccg tgttcatcgt cggggtaacg
     1501 atggtcggtg cgtatcaggc cgagaaggac gtccggatgg aggtcggcga cacggtcagc
     1561 gtcggcaact acactttccg cttcaacggc gtacggccgc agcagggacc caactaccgg
     1621 gccctgctcg gtgacatcga cctgatcaag gacggccggg tgctgcgcaa gatgttcccg
     1681 gagaagcgtt tctacgtcgc ttctgcgatg ccgatgaccg aagccgcgat cgatagcggc
     1741 ttgctgggcg acgtctacgt ctcgcttgga gagccgatcg acaaagccag gccggatgcc
     1801 gcgtgggcag tgcgcgtcta tcacaagcct ttcgtcgact ggatctgggg cggctgcgtg
     1861 ttgatggcct tcggcgggct gctggccatc agcgaccgcc gctaccggat caaggcacgt
     1921 gccggttcac ccgcctcatc tgccgccgcg tcgctggtgc aaaacgcctg aaggactgcc
     1981 catgaacagg tttctctggc cgttgatcgg cttcatcgtg ctggtcgtac tgctcgccgt
     2041 cgggctcaac ctcaaccctc gcgatgtccc ttcgccgctg gtcggaaaac ccgcaccgaa
     2101 cttctcgctg tcacaactcg cggcaccgga aaagaagttc tcgccgcagg acatgctcgg
     2161 caaggtctgg ctgctcaatg tctgggcatc gtggtgcgtc tcgtgtcgac aggaacatcc
     2221 ggtgctggtc gaactggcaa aaaaaggcac tgtccccttg gtcggtctca actacaagga
     2281 ggtccgcggt gacggcggca tcgacctcga caaggtcgct cccggcagcg aacaatcgat
     2341 ggtcatcgcg cgtgccagca cctggctgcg cacccacggc aacccgtaca ccctgtcggt
     2401 gctcgacatc gatggccgcg tcggcatcga ctacggcgtc tacggcgtac ccgaaaccta
     2461 tgtcatcgac aaggcaggga tcatccgcat gaaacatacc ggcccgatct cccctgacgt
     2521 gttctcgggc aagatcctgc cgctgctggc ggagctgtcg cgatgaagcg cttgctgctg
     2581 gtcgtgctgc tggcgatggc gggaacgggc tggcacagcc tccacgccgg agaagccaga
     2641 ccgctcgccg ccgacgaggc caccgagcaa cgcctgatcg ccattgcctc cgagctgcgc
     2701 tgcctggtct gccagaacga atcgctctcc ggttcaaacg ccgaactggc caacgatctg
     2761 cgccgcgaga tccgcggcct gatcagggac ggcaggagcg acgctgaaat catggagttc
     2821 atggtcagcc gctacggcga cttcgtgcgt tatcggccgc cgctgaaggg aagcacactg
     2881 ctgctgtggt tcggcccggg cctgctcttc gtgaccggcc tgacgtcgtt ggtgatctac
     2941 ctgcgccggc gcgaccgggc gatcaggaac acaccgctga cggctgcaga acaacagcgg
     3001 gccgaagcgc tgctgcagga ccgcgattca ggccagggac cgtcatgacc gctttcatca
     3061 tcgccgcggc gctgctgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3181 agacgcgcat cgccccggaa cagattctgg agatggtcaa cggcctcgtc gagaagctcc
     3241 gaaaaaaccc tgacgatagc cagggctggg tgatgctggc gcgttcatac aaggtcctgg
     3301 aacgttttcc cgaagcggcc gatgcttaca gccgcgccgc cacactggtc gaccaggacc
     3361 cggtcctgct cgccgattac gcggatgttc tcagccggag caatggcggc aaactacagg
     3421 gcaagccgac cgagttggtc gagcgggccc tcaagctcga ccccgacgag ccgcaggcgc
     3481 tgctgctggc cggtgccgca gcgaccgacc gacaggagtt cgctgccgcc gccgagtact
     3541 ggtcgcggct gcacgcccaa ctcgaacccg attccgagga agccagggcc ctcgcagccg
     3601 ccatcaacaa ggcacgcgag atcgccaccc aggccgctgc cgcgaagaca gcagggaagc
     3661 ctgcggccgg tgtcggcgcc gtcagtggcg aagtcaccct gagcagcagg cttgccgcgc
     3721 tggccaggcc ggacgacgtg ctgttcgtct ttgcgcgtgc agaggaaggg ccccgtatgc
     3781 cattggccgc catgcgggcg acggttgccg acctgccgtt gcgcttcatc tttgacgact
     3841 cgatggcgct ccccggcggc aggaagatct ccgatttcgc cacgatcagt atcgaggcgc
     3901 gcgtcgccaa agccggcaag gcccagagtt cgagtggcga tctgtcgggc agcctcagcg
     3961 gcatcaagcc gggtagccgc aatctgcgtc tgcagatcga ccaggttcag ccctgagccg
     4021 tcggccacgc catggcctgc aggccgacgc gatttgtccg atttgttaac ctagatcaaa
     4081 ccgtcggctc ttcctcctcc gctagactct agccagcctc ggcgctcggc agatggcact
     4141 agaaatccag ggaggggaga agcatcgtgt ccgacaatgt tccagggttc tggacaaagc
     4201 tcaaacgacc aagcacccgg tactcgctac tggcattgct ttcggtcggc ttcgtctgcg
     4261 gcgtcctttt ttggggcggc ttcaacaccg ccatggaagc caccaacaca ctcgacttct
     4321 gcatcagttg tcacgagatg cgggacaacg tttatcagga gtacaagacc accattcact
     4381 acagcaatcg caccggtgtg cgcgcttact gttcggattg tcacgtgccc aaggactggg
     4441 ggcacaagat gatgcgcaag attcaggcca gccaggaact ctacggcaag atcatgggga
     4501 cgatctccac gccagagaag ttcgaagcca agcgcctcga actcgcgacc catgagtggg
     4561 cgaggatgaa agccagcgat tcccgtgaat gccgcaactg tcactcgttc tcggcgatgg
     4621 acatcgagag acagaaggcg cgtgcttcca agatgcacaa gctcgggcag gacgacaaga
     4681 agacctgtat cgattgccac aagggaatcg cgcaccggaa acccaagaac atgcccgaag
     4741 aaccggacga cgactagcgg caatgacctc gaccgcggcg tcctggtccg cggccgaaga
     4801 cgaacgagtc acctgaggcc gactttggcc ggcgacaatc gcatcaacac ttgagtggag
     4861 tctgagatga aaagaacgag cctttccctc gccatactcg gcgtcgtgct gactttgggc
     4921 gcgccgatgg cgcaagctgc tccggactgg agcaaggttc ccaaacgcga tgtgcaggtt
     4981 tttcaccctg cggtaacgcc gatcgagtgg gtcatgaaga aatccgacca tagcggtcgc
     5041 actgggctca acaagggcga aagttgcgtc ggttgccacg aagagaaggg cggcctcaat
     5101 ttcgacatga agcgtctcgc cggcaaggac ctcgaaccca agggcgcacc caagacgatg
     5161 aatttcccgg tcagtgtgca atctgcctac gacaaggaca atgcctacat ccgtctgact
     5221 ttcaaggcac cgagtggagc cgtcgacccg gccgacaagg agaacgacgt caaggttgcg
     5281 gtgatgtttg ccaacgacaa agtcacccag ggcgcacaga tcggctgctg ggcgacctgt
     5341 cacagcgatg cgcgcaccat gcccggcgcc gacgagaaga aaaccaagta caccaaggac
     5401 ggcgcctacg aactgatgca gtggaagagt gccaagggtg ccaaggtcgc cgacggctcg
     5461 gtgaccgaca agcgcaacat ggaaggcggc accgccggcg tcaaggccga agggagcaag
     5521 gtgggcgaga cctacaccgt caccttcaca cgcaagctct cgggaactct ggcagagggc
     5581 aaggcgacac ccttcggcgt cgccattcac tctgaccatg ccagtggccg cttccatcac
     5641 gtttccatgg gatataccat gggcttcggg gccgacggcg acatcaagtc gaccaaacag
     5701 taaggctctc tccc
//
LOCUS       DAOSIT010000079         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12740_length_5700_cov_7.970239, whole genome shotgun sequence.
ACCESSION   DAOSIT010000079 DAOSIT010000000
VERSION     DAOSIT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12740_length_5700_cov_7.970239"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLL14_03105"
     CDS             complement(<1..215)
                     /locus_tag="PLL14_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG02940.1"
                     /translation="MPRRRITFRQLETFTTVAHMRSFSKAAAVLHLTQPAVSLQIRQI
                     VETLGLPLFEQSGREVVLTAAGEELLQT"
     gene            333..800
                     /locus_tag="PLL14_03110"
     CDS             333..800
                     /locus_tag="PLL14_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02941.1"
                     /translation="MRNYTTDSAEAMSRVVALALLADGALDHCELASLEKHEIYGRLR
                     IDAATLDQVIHEFCNDLMQCARAPNVGQIELDHELIDHLLGDIRSPELQKEALRAIVD
                     IVNADGCLNGGEGILLSQAKHLWEQDLQQDNTAESAKPQPTLPLARHQKQWFS"
     gene            complement(856..1614)
                     /locus_tag="PLL14_03115"
     CDS             complement(856..1614)
                     /locus_tag="PLL14_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02942.1"
                     /translation="MSTLPEQPERTVVLEVRGLSVNYGKVEALHGLALRVHRGEIVTV
                     IGPNGAGKTTLLSALIGLLPVRGEIVYLGERQGAGRSVEQLVRQGMTLVPEKRELFAE
                     MSVEDNLLLGAFDRYRAGHRDHLETMDQVFDTFPRLMERRNQMAGTLSGGERQMLAMG
                     RALMAKPRLLLLDEPSLGLAPLVIKEIFRTVAQLKRLGVAILLVEQNARAALQVADYG
                     YVLETGELSLEGPSSELSTDRRVIESYLGLGGRL"
     gene            complement(1611..>2266)
                     /locus_tag="PLL14_03120"
     CDS             complement(1611..>2266)
                     /locus_tag="PLL14_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02943.1"
                     /translation="IGPNGAGKSTMFNGISGVDPLTSGSVSFRGEPIEGLPAREIARR
                     GMSRTFQHVRLLPSMSVIENVAIGAHLRGRRGFVAAACRADRAEEARLLHEAAVQIER
                     CGLPEQMYEAAGSLPLGKQRIVEIARALAADPCLLLLDEPAAGLRYLEKQALADLLRR
                     LRAEGMGILLVEHDMDFVMGLADRVLVMEFGQKLAAGRPEEIQRNPAVIEAYLGGVE"
     assembly_gap    2267..2366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2367..3145)
                     /locus_tag="PLL14_03125"
     CDS             complement(<2367..3145)
                     /locus_tag="PLL14_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG02944.1"
                     /translation="MRRSVQSTLLLLLFIVVLLLAPVLLPEFYVTLLNYIGLYAIVAL
                     GLVMLTGVGGLTSFGQAAFVGLGAYTSAYLTTVHGVSPWLTLPAGLLVTGVVALSLGL
                     VTLRLSGHFLPLGTIAWGMSLYYLFGNLDALGGHTGMSGIPPLQFVELELKSGREFFY
                     LIWAVLLVLVLGTRNLLDSREGRAIRALKGGSVMAEAMGVNTPRSKIVVFTLAALYAS
                     TSGWLYAHLQRFVNPTPFSLTQGIEYLFMAVVGGVSHVWGA"
     gene            complement(3142..4176)
                     /locus_tag="PLL14_03130"
     CDS             complement(3142..4176)
                     /locus_tag="PLL14_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG02945.1"
                     /translation="MDLQIALLLGQDGVTNGAIYALLALALVLVFAVTRVIFIPQGEF
                     VAFGALTLASLQAGRLPGAVWLLLAMGTLIALIEGAAALRERHFRRIPLILLLNCGAP
                     LLLAGALVAVSSASLPLLVQVFLALLLVVPLGPMIYRLAYQPVAEASVLVLLIVSVAV
                     HVTLIGIGLLFFGAEGSRTPSFTEVSFEVGDVLLQGQTLLVLVASVLLIIALYFFFER
                     TLYGKALRATAMNRTGARLMGISGSLAGMLSFTLAASIGAVSGILISPITTIYYDSGF
                     LIGLKGFVGAIIGGLASYPVAAAGAILVGLLESYSSFWASAFKEVIVFTLIIPVLLWR
                     SLTTRHVEGE"
     gene            complement(4270..5424)
                     /locus_tag="PLL14_03135"
     CDS             complement(4270..5424)
                     /locus_tag="PLL14_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG02946.1"
                     /translation="MKINASMAGLIALLAAAFAANVQADINVGVTLSATGPAASLGIP
                     EKNTVALLPTTIAGQKVNYIVLDDASDTTTAVKNTRKLIAENKVDVIIGSTITPNSLA
                     MIDVVAEAGTPMIAMAASARLIEPMDAKRYWVFKTPQNDAQMATAIVEHMTNNNVRTV
                     AYIGFADAYGEGWWNEFSKIAEVRKIQIVANERFNRADTSVTGQVLKILAAKADAVLI
                     GGAGTPAALPQKSLKEKGYKGIMYQTHGVANADFLRVCARDCEGTFLPAGPVLVAAQL
                     PDANPIKKAALEYVGKYEATYGKGSVSTFGAHAWDASKLLANAVPEALKKAQPGTPEF
                     RQALRDGLEKTRNLTAAHGVFNMSPQDHLGFDQRARVMVKVEGGSWKLVQ"
     gene            complement(5517..>5700)
                     /locus_tag="PLL14_03140"
     CDS             complement(5517..>5700)
                     /locus_tag="PLL14_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG02947.1"
                     /translation="FFIPHGHFLPMLALLMATCNVLGSFLGAHLALRHGSGFVRKTFL
                     CVVSLFILKFAHDTFV"
BASE COUNT         1231 a         1773 c         1647 g          949 t
ORIGIN      
        1 gtctgcagca actcctcccc cgccgcggtc aggacgacct cgcggcctga ctgctcgaac
       61 aacggcagac ccagagtttc gacgatctgc ctgatctgca gtgaaacggc tggctgtgtg
      121 aggtgcagga cagcggctgc tttggaaaag ctgcgcatgt gcgccactgt ggtgaaagtt
      181 tcgagttggc gaaaagtgat tcggcggcgt ggcatatata agtctgaaca aatggattat
      241 gcgatgatat ctcattggac tttatcacgt tgcgctagca tagttcaccc atccaaagcg
      301 tcgtatcaga tcaaccacct caggagaaac gtatgcgcaa ttacaccacc gacagcgctg
      361 aagccatgtc acgagtcgta gccctcgccc tgctcgccga tggtgcgctt gaccactgcg
      421 aactcgcgtc gcttgaaaaa cacgaaatct acggcaggct gcgcatcgac gccgcaaccc
      481 tggatcaggt gatccacgaa ttctgcaatg acctgatgca atgcgctcgc gcgcccaatg
      541 tcggtcagat cgaactggat catgaactga tcgatcacct gctcggtgac atccgttcac
      601 ccgaactgca aaaagaggca ctacgggcga ttgttgacat cgtaaatgcc gacggctgcc
      661 tcaacggcgg cgaggggatt ctgctctccc aagcgaaaca cctctgggaa caggatctgc
      721 aacaggacaa taccgccgaa agtgctaaac cacagccgac gttaccgctg gcccggcatc
      781 agaaacagtg gttctcgtga agacagcctg gccgcctcgc ctgcggagcg gccgagccgc
      841 ccaggttgcg aggggctaga ggcggccccc aagacccagg taactctcga tcacccgccg
      901 atccgtagac aattcgctgc tcggcccttc cagggacaac tctccggttt ccaggacata
      961 gccgtagtca gcaacctgca acgcggcccg ggcgttctgc tcgacgagca ggatcgccac
     1021 gcccaaccgc ttcagctgcg cgacggtacg aaaaatttcc ttgatgacca aaggtgcgag
     1081 tccaagactc ggttcatcga gcagcagcag cctgggtttg gccatcagcg cccgacccat
     1141 ggccagcatc tgacgctcgc cgccagaaag cgtgccggcc atctggtttc gacgctccat
     1201 caggcgtgga aaggtgtcga acacctggtc catggtttcc agatgatcgc gatgacctgc
     1261 ccgatagcgg tcgaaggctc ccaagagcag gttgtcctcg acgctcatct cggcaaagag
     1321 ttcgcgtttc tccggaacca gggtcatccc ttgacgcacc agttgctcga cgctacgccc
     1381 cgccccttgc cgctcaccga gatagacgat ctcgccacgt acgggaagca gtccgatcag
     1441 ggccgacagg agggtggtct tgccggctcc gttcggaccg atgaccgtga cgatctctcc
     1501 ccggtgtacc ctgagggcaa gaccatgcag agcctccacc ttgccgtaat tcactgacaa
     1561 gccgcgcacc tcaaggacga ccgttctttc gggctgctcg gggagcgtac tcattccacg
     1621 ccgcctaggt aggcttcgat aaccgcaggg ttccgctgga tctcctcagg ccgcccggca
     1681 gcaagttttt gcccgaactc catcaccagc acccggtcgg cgagacccat cacgaaatcc
     1741 atgtcgtgct cgaccagcag gatacccatg ccttccgcgc gcaaacgccg cagaaggtcg
     1801 gcgagcgcct gcttttcgag gtagcgcaga ccggcagccg gttcgtcgag cagcaacagg
     1861 caagggtcgg ctgcgagcgc acgggcaatc tcgacgatgc gctgcttccc gaggggcagg
     1921 ctgccggctg cttcgtacat ctgttcgggc agaccacagc gctcgatctg gacagccgct
     1981 tcgtgcagca ggcgggcctc ctcggcacgg tcggcgcggc aggcggcggc gacaaaaccg
     2041 cggcggccgc gcaggtgtgc gccgatggca acgttctcga tcacgctcat cgatggcagg
     2101 aggcgtacat gctggaaggt ccggctcatc ccgcggcgcg cgatctcacg tgccggcaga
     2161 ccctcgatag gctcgccgcg aaagctgacc gaaccggagg tcagtgggtc aaccccggaa
     2221 atgccattga acatcgtact cttgcccgcg ccgttcggcc cgatcannnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnatcg caccccagac gtgcgagacg ccgccgacca
     2401 cggccatgaa cagatattct atgccttgtg tcagcgagaa cggcgtcggg ttgacgaagc
     2461 gctgcagatg cgcataaagc caaccggaag tgctggcgta gagtgccgcc agcgtaaaca
     2521 ccacgatctt cgagcgcggg gtgttgaccc ccatggcttc ggccatcacc gagccaccct
     2581 tcagcgcccg gatggcgcgg ccttcacgcg aatcgagcag gttgcgcgtc ccaagaacga
     2641 gcaccagcag gaccgcccag atcaggtaga agaactcgcg ccccgacttg agttccagtt
     2701 cgacgaactg caggggcggg atgccgctca tgccggtgtg gcctcccagc gcatcgagat
     2761 tgccaaagag atagtagagg ctcatccccc aggcaatggt acccagcggc agaaagtggc
     2821 cggacaggcg caaggtgacc aggccgagcg agagggccac cacgcccgtg accagcagac
     2881 ccgcaggcag cgtcaaccac ggcgaaacac catgtacggt ggtcaggtag gccgaagtgt
     2941 aggcgccaag gccgacgaac gcggcctgcc cgaaggaagt caggccaccc acaccggtga
     3001 gcattaccag tccgagcgcg acaatggcat agagaccgat gtagttgagc agcgtgacgt
     3061 agaattcggg caacagcacc ggcgccagca gcagcacgac gatgaagagc agtaacagca
     3121 gcgtcgactg cacgctacgc ctcattcccc ctccacatgt cgcgtcgtca gcgaacgcca
     3181 gagcaacacc gggatgatca gggtaaacac gatcacctcc ttgaaggcgc tggcccagaa
     3241 cgaggaatag gattcgagca gaccaaccag gatggcaccg gcagcggcca ccggataact
     3301 ggcaaggccg ccgatgatcg cgccgacaaa tcctttcagg ccgatcagaa aacccgagtc
     3361 gtagtagatg gtggtaatcg gtgaaatcag aatgcctgaa acagcaccga tcgatgccgc
     3421 cagggtaaac gacagcatcc cggccaggct tcccgagatc cccatcaggc gcgcgccggt
     3481 gcggttcatc gccgtggcgc gcagggcctt gccgtagagc gtgcgttcaa aaaagaaata
     3541 cagggcaatg atcaacagca cgctggcgac cagtaccagt agcgtctggc cttgcagcag
     3601 cacatcgccg acctcgaagc tcacttcggt gaatgacggc gtgcgcgaac cctcggcacc
     3661 aaagaaaagc agcccgatgc cgatcagcgt gacatgtacg gcaacggaaa cgatcagcaa
     3721 caccagcacg gacgcttccg caaccggctg ataggccagg cgatagatca tcggcccgag
     3781 cggaacgacc agcagcagtg ccagaaacac ctgcaccaac agcggcagac tggccgaaga
     3841 aaccgccacc agagccccgg ccagaagcag cggcgccccg caattgagca acaggatcag
     3901 gggtatgcgc ctgaaatgcc gttcccgcag cgccgccgct ccttcgatca gggcaatcaa
     3961 tgtacccatt gccagcaaca gccagacggc ccccggtagc cggccagcct gcaggctggc
     4021 cagggtcagg gcgccgaaag caacaaactc cccttgcggg atgaagatca cccgcgtgac
     4081 ggcgaaaacc agtaccagtg ccagcgcgag caacgcatag atggcgccgt tggtaacccc
     4141 atcctggccc agcagtaagg caatctgcaa atccatcgac actattccgg tcgccaccct
     4201 gtgtggtggc gaggatgata cccgactaag ggcgggacct ggcgacgcaa ccgcggcgcg
     4261 ccatgcgtct tattgcacca gtttccagct gcccccttcg accttgacca tcacgcgagc
     4321 gcgctggtca aaccccagat gatcttgcgg actcatgttg aaaaccccgt gcgccgcagt
     4381 caggttccgg gtcttctcga gcccgtcgcg tagcgcctgg cggaactctg gtgtacccgg
     4441 ctgcgctttc ttcaacgcct cgggcacggc attggccaac agcttgctgg catcccaggc
     4501 gtgcgcgccg aaggtggaca cactaccctt gccataggtt gcctcgtact tgccgacgta
     4561 ttcaagcgcc gctttcttga tcgggttggc atcaggtaac tgcgcggcga ccagcaccgg
     4621 gccggccggc aggaaggtgc cttcgcagtc cctggcacag acccgcagga aatcggcatt
     4681 ggccaccccg tgcgtctggt acatgatccc cttgtagccc ttttccttga gcgatttctg
     4741 cggcagcgcc gcgggcgtcc cggcaccgcc gatcagcacc gcatccgcct tggcggcaag
     4801 gatcttcagc acctgaccgg tgaccgatgt gtcggcacga ttgaatcgtt cgttggccac
     4861 gatctgaatc ttgcgcacct cggcgatttt cgagaactcg ttccaccagc cttcgccgta
     4921 ggcgtcggca aagccgatat aggcgaccgt tctgacgttg ttgttggtca tgtgttcgac
     4981 aatggcggtc gccatctggg catcattctg cggggtcttg aacacccagt agcgctttgc
     5041 atccatcggt tcgatgaggc gcgcagaagc ggccatggca atcatcggcg tgccggcttc
     5101 ggcgacaacg tcgatcatcg ccagcgagtt gggtgtgatc gtcgaaccga tgatcacatc
     5161 gaccttgttc tccgcgatca gcttgcgggt gttcttgacc gcagtggtgg tgtcggaggc
     5221 atcatcgaga acgatatagt tgaccttttg acctgcgatg gtcgtcggta acagcgccac
     5281 ggtatttttc tccgggatac ccagcgaagc cgcagggccg gttgccgaaa gcgttacccc
     5341 gacattgata tcggcttgaa cgttggcagc aaaggctgcc gccagcaggg caataagccc
     5401 ggccatcgat gcattgatct tcatggtccc cccaacacga ttttccggtg cgcaaaggct
     5461 gcagactggc ccgcagcaaa caggcattgt gcggccgcag gaaccgcgag acaagtctaa
     5521 acgaaggtat cgtgcgcgaa tttgaggata aagaggctca ccacgcacaa aaacgtcttc
     5581 cgaacgaaac cactgccgtg gcgcagtgca aggtgcgcgc cgagaaacga accgagcaca
     5641 ttgcaggtcg ccatcagtaa cgccagcatg ggcaaaaagt gaccgtgcgg gatgaagaag
//
LOCUS       DAOSIT010000080         5682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12794_length_5682_cov_8.497245, whole genome shotgun sequence.
ACCESSION   DAOSIT010000080 DAOSIT010000000
VERSION     DAOSIT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12794_length_5682_cov_8.497245"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..397)
                     /locus_tag="PLL14_03145"
     CDS             complement(146..397)
                     /locus_tag="PLL14_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOG02948.1"
                     /translation="MRETTRLTLVSRHYCHLCQEMELALSALLADFPIDLEVLDVDAD
                     PLLEASYDELVPVLLHDGKELCHYFLDVAKVRDYLSEIR"
     gene            complement(394..1833)
                     /locus_tag="PLL14_03150"
     CDS             complement(394..1833)
                     /locus_tag="PLL14_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOG02949.1"
                     /translation="MKQLLAAFFLATFSLLAQAQNRGLPDFTELVEKQGPAVVNISTT
                     QTARSSGRSGQPLPFDENDPMNEFFRRFIPRQPGMPGFPGAPRDFESRSLGSGFVVSA
                     DGYILTNAHVVDSADEILVRLTDKRELKARVIGTDKRTDVALIKIEATGLPTVRLGDP
                     GTLKVGEWVVAIGSPFGFDNSVTAGIVSAKGRSLPQENYVPFIQTDAAVNPGNSGGPL
                     FNLKGEVVGMNSQIYSRSGGFMGISFAIPIDVAMEVQAQLRASGKVSRGRIGIVIQEV
                     NKDLADSFGLSKAQGAAVNAVEKGGPAEKAGIEPGDVILKFDGKAIVNSSDLPRIVGS
                     TRPGSRVMIQVWRKGSSRDLPVVVGEITEEKVAARSNRGARPVERTANRLGLVVSELT
                     AEQKRELKVNGGLLIEDVRSNAVRADLRPGDVILALISRGESTEIKSAEQFNRLLAQF
                     DKAANVTLLVRRGEMQTFVTIKGLTERRE"
     gene            complement(1912..2328)
                     /locus_tag="PLL14_03155"
     CDS             complement(1912..2328)
                     /locus_tag="PLL14_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG02950.1"
                     /translation="MTEAWGTITAVDGDHATIRMDEAGCGRCGEPGGCGGNIHGKLFC
                     STPRTFRVPNPDHCAVGQRVRVGVFAGSLRRSAVHAYALPLLMLLAGALTGSACAGEV
                     GAIAGAIAGLFVGWLGLRRVQRQSLRNPHLQPTIRS"
     gene            complement(2454..>3008)
                     /gene="rpoE"
                     /locus_tag="PLL14_03160"
     CDS             complement(2454..>3008)
                     /gene="rpoE"
                     /locus_tag="PLL14_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOG02951.1"
                     /translation="AGDKHAFELLVAKYQRKVARLLSRFIRDQSEVEDVAQEAFIKAY
                     RALPAFRGDSAFYTWLYRIAINTAKNYLVAQGRRAPTSTEFDAEEAEGFEDAGRLRDI
                     NTPESLLQSKQTGETINAAMAALPEELRSAIVLREIEGLSYEEIAEAMRCPIGTVRSR
                     IFRAREAVAEKLRPLLDTSVDRRW"
     assembly_gap    3009..3108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3109..4025)
                     /locus_tag="PLL14_03165"
     CDS             complement(<3109..4025)
                     /locus_tag="PLL14_03165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG02952.1"
                     /translation="MSRWQFRPQPLKFQLGDWTLFSIPLRLQILAVRLIDETPPVALP
                     DLPPEPLAAGSQGYLIRGLPVATELPVISQVEGYLCYVPLQYQHCYIDLGMSFESYQK
                     KFSSKTRSTISRKVRKYAEHCGGTVAWKTYAMPDEMREFFRLARAVSQKTYQERLLDA
                     GIPDTEDFIAHAESLAAEQRVRAYLLFDGERPVSYLYCPVHDGVLTYAYLGYDPDYMN
                     MSVGTVLQWLAVEQLFREGRFRYFDFTEGQSEHKRLFATHQRQCANVFLVRRTLGNTM
                     LVYSHLGMERFSKWLGATLERFGIKAKIK"
     gene            complement(4022..4987)
                     /locus_tag="PLL14_03170"
     CDS             complement(4022..4987)
                     /locus_tag="PLL14_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011331092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOG02953.1"
                     /translation="MLDVFFTVDVEVWCDGWENLDERFPNAFRRYVYGPTTRGNYGLP
                     YQVEVLNAHGLTGVFFVEPLFSTRFGPGPLAEIVGLLREGAQDVELHLHTEWVDEARE
                     PLLPDSHGKRQHLRYFTLDEQLVLISLGAKLIEQSGGGEVSAFRAGSFAFNRDTLRAL
                     SKSGIPFDSSYNASQFGADSGVMTGLTVVEPIECEGVHEYPMTVFDDGSGSLRHAQLT
                     ACSYGELESLLWKALETERSAFVILSHNFELLNEAKNRPDGVVVKRFRKLCSFLDRNR
                     DSFRVRPFHGLQARTAPQQQPSPLRSPIWRTGARILEQAYRRRYG"
     gene            complement(5117..>5682)
                     /locus_tag="PLL14_03175"
     CDS             complement(5117..>5682)
                     /locus_tag="PLL14_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG02954.1"
                     /translation="AGLSFLTKLSTGYWQIFEPTNGFTAVSRQALERCDLARVSKRYF
                     FESDLLYHLALARVVVIDFPRASDYADEQSSLRPMRIIGTFLRGHLANFARRVLYTYF
                     VRGFSLASIELIIGMLLILFSIVFGVSEWIASAQSGAPATAGTVMLAALPAILGTQLT
                     LSWLHFDIHSEPRIPITLQATKVRAAE"
BASE COUNT         1100 a         1700 c         1663 g         1119 t
ORIGIN      
        1 gacttgccgt gatcgatatg cgcgatgatg gaaaaattgc ggatgtgttg catggacatc
       61 gtgtgggctc cgttgccagc gagccgtccc tgaaaaaaag ggtgccagcg agcacccttg
      121 gtcgaactgc ctagagggcc ggattctagc ggatttcact caaataatca cggactttgg
      181 caacatcgag gaagtagtgg caaagctcct tgccgtcgtg caggaggacc ggaacgagtt
      241 cgtcatagct ggcctcgagc agcggatcgg cgtcgacgtc gagcacttcg aggtcgatgg
      301 ggaagtcagc cagcaaggcg ctgagcgcga gttccatctc ctgacagaga tggcagtaat
      361 gacgcgaaac cagcgtcagc cgggtcgtct ccctcattcc cgtctctcgg tcaacccctt
      421 gatggtgacg aaggtctgca tctcgccacg gcgcaccagc agggtcacat tggccgcctt
      481 gtcgaattgc gcgagcaagc gattgaactg ttcagcgctc ttgatttcgg tgctctcgcc
      541 gcgcgaaatc agcgccagga tgacgtcgcc tggccgcagg tcggcgcgca ccgcgttgct
      601 gcgcacatcc tcgatgagca aaccgccatt gaccttgagc tcgcgtttct gctctgccgt
      661 gagttccgac accaccagtc cgaggcgatt ggctgtcctt tcgaccggtc tggcccccct
      721 gttgctgcgt gctgcgacct tttcttcggt gatttcaccg actaccaccg gcaggtcgcg
      781 gctgcttccc ttgcgccaca cctggatcat cacccggctt cccggcctgg tactgccgac
      841 gatgcgtggc aagtcgctgg aattgacgat ggccttgccg tcgaacttca ggatgacatc
      901 gccgggctcg atgcccgctt tctccgccgg gccgcccttt tcgacggcgt tgacggctgc
      961 gccctgggct ttgctcaaac cgaatgagtc ggccagatcc ttgttgacct cctgaatgac
     1021 gatcccgatg cgaccgcggc tgaccttgcc gctggctcgc aactgcgcct gcacttccat
     1081 cgccacatcg atcgggatgg caaaggaaat ccccatgaaa ccacctgagc ggctatatat
     1141 ctgagagttc atgcccacca cctcaccctt gaggttgaac agtggcccgc ccgaattgcc
     1201 cggattcacc gccgcatcgg tctggatgaa gggcacgtag ttctcctgtg gcaacgagcg
     1261 tcccttggcg ctgacgatgc ccgcggtcac actgttgtcg aaaccaaagg gtgaacctat
     1321 cgcgactacc cattcgccga ccttgagagt tccgggatcg cccagacgaa cagtcggcag
     1381 acccgtggct tcgatcttga tcagtgccac atcggtccgc ttgtcggtgc cgatgacccg
     1441 cgccttgagt tcacgcttgt cggtcagacg aacgaggatt tcgtctgccg aatcgacaac
     1501 gtgcgcattg gtgagaatgt agccatcggc actgaccacg aaccccgagc cgagagagcg
     1561 actctcgaaa tcgcgcggcg cgccgggaaa tcccggcatc cccggctggc ggggaatgaa
     1621 gcgccggaag aactcgttca tcggatcgtt ctcatcgaaa gggaggggct gcccactccg
     1681 cccactgctg cgggccgtct gggtggtgct gatattgact accgccggtc cctgtttttc
     1741 gacgagttcg gtgaagtcgg gaagcccacg attctgagcc tgcgccagca gggaaaatgt
     1801 cgccaaaaag aaagccgcaa gcaactgttt catggcacca aatgcctcct gatgtctttg
     1861 agtatcgaac cagcatcaaa ccagacgaaa ctgcggacac acgccgcaaa atcaagaccg
     1921 gatcgtcggc tgcaggtgag ggttacgcaa gctctgtcgt tgcacgcgtc gcaaacccag
     1981 ccagccgacg aacaggccgg cgatcgcgcc ggcgatcgcg ccgacttcgc cggcgcaagc
     2041 cgagcccgtc agcgctccgg ccaggagcat gagcagggga agagcgtagg catgtacggc
     2101 gctgcgccga agcgagcccg cgaagacacc aacccgcacc cgctgaccga ccgcgcagtg
     2161 gtcgggattg ggcacccgga aggtgcgcgg tgtgctgcag aacagtttgc cgtggatgtt
     2221 gccgccacag ccacccggtt ccccgcagcg accgcaaccg gcttcgtcca tgcgtatcgt
     2281 cgcatgatcg ccgtcgacag cagtaatcgt tccccacgcc tcagtcatca acagggttcg
     2341 ctcacgacat atccttgctc gccgccacta ttcctgacga cgcacactga cctggctcgg
     2401 catcaagatt ggcaggcgct ccaggggctc tcggcccaac tctcgcatgg gggctaccac
     2461 ctgcgatcga ccgaggtatc gagcagtggc cgcagcttct cggcgaccgc ctcgcgcgcg
     2521 cggaaaattc gcgaacgtac cgtgccgatc ggacaacgca tcgcctcagc aatttcctca
     2581 taactcaaac cttcgatctc acgcagcacg atggcgctac gcaactcctc cggcagggca
     2641 gccatggccg cattgatcgt ttccccggtc tgcttggact gcagcaggct ttccggggtg
     2701 ttgatgtcgc gtaggcggcc ggcatcctca aaaccttcgg cttcttcggc gtcgaattcg
     2761 gtcgacgtcg gcgcgcggcg tccctgggcg acgagatagt tcttggccgt gttgatcgca
     2821 atccggtaca gccaggtata gaacgcactg tccccacgaa acgcgggcag cgcccgataa
     2881 gccttgatga acgcttcctg cgcaacgtcc tcgacctcgg actggtcgcg tatgaagcgc
     2941 gacaacaaac gtgcgacctt gcgctggtat ttcgctacca gcaactcgaa cgcatgttta
     3001 tcgcccgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg tttgatcttg
     3121 gcttttatcc cgaaacgttc cagcgttgca ccgagccact tcgaaaacct ttccatgccc
     3181 agatgactgt atacgagcat cgtgtttccg agggtgcgcc gcaccaggaa gacgttggca
     3241 cattgccgtt ggtgggtcgc gaagaggcgt ttgtgttccg actggccttc ggtgaagtcg
     3301 aagtagcgaa agcgtccttc cctgaacagt tgctccaccg ccaaccattg caggaccgtg
     3361 cccactgaca tgttcatata gtctggatcg tagccgaggt aagcataggt gagaacgcca
     3421 tcgtgcaccg gacagtaaag atacgaaacc ggtcgttcgc cgtcgaacag gaggtaggcg
     3481 cgtacccgtt gctcggcggc gagcgactcg gcgtgcgcga taaaatcctc ggtgtcggga
     3541 atcccggcat ccagcagtcg ttcctgatag gtcttctggg acaccgcgcg ggcgagccgg
     3601 aagaactcgc gcatctcgtc gggcatggcg taggtcttcc aggcgacggt accgccgcag
     3661 tgctcggcgt acttcctgac cttgcgactg atcgtggacc gggttttcga ggagaatttc
     3721 ttctgatagc tctcgaaaga cattcccaga tcgatgtagc agtgttgata ctgaagaggg
     3781 acgtagcaga ggtagccttc gacttggctg atgacgggca gttcggtcgc cactggcagt
     3841 ccccggatca aatatccctg gctgcctgcg gcgagcggtt cgggcggcag atccggcagc
     3901 gctaccgggg gcgtttcgtc gatcagtcgt actgccagga tctgtagccg aagcggtatg
     3961 ctgaagagcg tccagtcgcc gagttgaaac tttagaggct gtggacgaaa ttgccaacga
     4021 ctcatccgta cctcctgcgg taggcctgtt ccagtattct tgcgccggtc ctccagatcg
     4081 gcgaccggag aggggagggc tgttgctgtg gtgcggtccg ggcttgcagg ccgtggaagg
     4141 gacgcacgcg aaagctgtcc cggttgcggt cgagaaacga gcacagcttg cgaaagcgct
     4201 tgacgaccac cccgtccggc cgattcttgg cctcgttgag taactcgaag ttgtgggaaa
     4261 ggatgacaaa ggcgcttctt tcggtctcca gagccttcca tagcaggctc tccagttcgc
     4321 cgtaagagca ggcggtgagt tgggcgtgcc gcagggagcc gctgccgtcg tcgaacaccg
     4381 tcatcgggta ctcatgtacg ccttcgcatt caatgggttc gacgaccgtc aggccggtca
     4441 ttacgccgct gtctgcgcca aactggctgg cgttgtagct gctgtcgaag ggaatgccgc
     4501 tctttgacag cgcccgcagc gtatctctgt tgaacgcgaa actgccggcg cggaaggccg
     4561 agacctcgcc ccctcccgat tgttcgatca gcttggcgcc caggctgatc agcaccagtt
     4621 gctcgtccag cgtgaagtag cgcaggtgtt gcctcttgcc atgggagtca ggcagtaggg
     4681 gctcccgcgc ttcgtccacc cattcggtgt gcagatgaag ttcgacatcc tgggcgcctt
     4741 cgcgcaggag tccgacgatc tccgccagag gaccaggtcc gaagcgcgtc gagaataggg
     4801 gttcgacgaa gaacacgccg gtcaacccgt gcgcattcaa cacctctacc tgataaggca
     4861 gaccgtagtt acctctggtt gttgggccat agacgtaacg ccgaaaggcg ttcggaaaac
     4921 gctcgtcgag gttttcccaa ccatcgcacc agacttcgac gtccaccgtg aaaaagacat
     4981 ccagcatctc gttgagcctt ctcgctattg aagtcgagct gctcgacgcc actctcgacg
     5041 gagcaattcg tacggcgggc cggtgctcgg accaacgcca ggtagctttg cgtgttgcag
     5101 tccggtgttc ttgctcttac tccgctgctc gcactttcgt ggcttgcagt gtaatcggaa
     5161 tgcgtggctc actatggata tcaaagtgca gccaggatag cgtgagttgg gtacccagga
     5221 tagccggtag cgcggcaagc ataaccgttc cggccgttgc cggggcaccg ctctgcgctg
     5281 acgcgatcca ctcgcttact ccgaagacga tgctgaacag gatcaacagc atgccgatga
     5341 tcaactcgat cgaggccagg gaaaatccgc gcacaaagta ggtgtaaagc acgcgtctcg
     5401 cgaaattggc cagatgtccc ctgaggaacg tgccgatgat gcgcatcggt cgcagactcg
     5461 attgttcgtc tgcgtagtcc gaagccctcg ggaagtcgat gactaccaca cgcgccagcg
     5521 ccaggtgata gagcaggtcc gactcgaaaa aatatcgctt gctgacccgc gccaggtcac
     5581 agcgctcgag cgcctggcgc gataccgccg tgaatccgtt ggtcggttcg aatatctgcc
     5641 agtagccggt cgacagtttg gtcagaaagg acagtccagc gt
//
LOCUS       DAOSIT010000081         5667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12833_length_5667_cov_10.167855, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000081 DAOSIT010000000
VERSION     DAOSIT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12833_length_5667_cov_10.167855"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLL14_03180"
     CDS             complement(<1..233)
                     /locus_tag="PLL14_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02955.1"
                     /translation="MHSLLQSVPDRLPLDCRTTAEVLNRGCACISVDHESLKRALEAG
                     DGTLSHAQLQATRPHLFADSMVFVSAEHLARMAR"
     gene            complement(239..2176)
                     /locus_tag="PLL14_03185"
     CDS             complement(239..2176)
                     /locus_tag="PLL14_03185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOG02956.1"
                     /translation="MTALLCEVFSADGPLAGAIPGYRPRAQQLELAEHIAAAMNDYRV
                     LVAEAGTGTGKTYAYLVPALLSGGKVIVSTGTRNLQDQLFTRDIPTIRKALKSPVKVA
                     LLKGRANYLCHHHLERSLADGRFLSRDDAAYAQRIARFSRQTDSGDKAECADVPENAA
                     VWAMVTSTRDNCLGQDCPRHKDCFVLAARREALAADLVVVNHHLFFADVMLRDEGTAE
                     LLPACNTVIFDEAHQLPEVASLFFGDSLSTAQLLELTRDTQLEARASARDCLDLPSVC
                     GALEKAARELRLTLPVEPARYALLQLEARPGFAPGMLRVIDELEVLAALLETQAQRSE
                     GLESCWRRATELLARLQSWQAGSNGDFVRWGETHTHSLQLNATPLVIAEIMQKQMSGH
                     PRAWIFTSATLAVQDDFAHFCAEMGLTGACSARWESPFNYAQQALLYVPRNLSDPNSA
                     DYAEAVVRAAFPVLKASGGRAFFLCTSLRAMRRTHALLVEWLARESLDLPVLLQGERS
                     KNELLERFRRLGNAILIGSQSFWEGVDVRGEALSLVVIDKLPFAPPDDPVLSARIERL
                     KGQGRNAFMEYQLPRAVINVKQGAGRLIRDETDRGVLMICDPRLVSKPYGKRIWRSLP
                     PMKRTRELAEALAFFAVGSTA"
     gene            complement(2373..>3035)
                     /locus_tag="PLL14_03190"
     CDS             complement(2373..>3035)
                     /locus_tag="PLL14_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOG02957.1"
                     /translation="VVVVGTYQLTRSPQFVMRGTGFVVGDGREVATNAHVIQEPLDLN
                     AGEMLVVVARLSAAATQQRRVRDFAVDKAHDLALLRLDGQALPALALHDSEPVREGQS
                     VAFTGFPIGGVLGLSPVTHRGIISSITPIVLPGANARQLNARVVQQIRSGSFDIYQLD
                     ATAYPGNSGGPLYEVNRGEVIGIINMVFVKENKESILSKPSGISFAIPVRFLRELMQK
                     GP"
     assembly_gap    3036..3135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3136..3854)
                     /locus_tag="PLL14_03195"
     CDS             complement(<3136..3854)
                     /locus_tag="PLL14_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG02958.1"
                     /translation="MLIAGEIPREADAERLLALRKEAVAHEHGEGVPRDYARAAKLYC
                     DGARLGDPEAQFSLGWMYANGRGIERDDARAAYFFSLAAKQGHAPSQRMQRFVGEPGS
                     AVPECMRAVVFVDESPDVMLPSSEAQKKVLGLVNQLAPEYGVSPRLALAVIRAESNFN
                     PMALSNKNAQGLMQLIPDTSARFNVKKPFDPEQNLRGGLAYLRWLLAYFEGDVSLVAA
                     AYNAGEGAVNRYRGVPPYAETR"
     gene            complement(3932..4981)
                     /locus_tag="PLL14_03200"
     CDS             complement(3932..4981)
                     /locus_tag="PLL14_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG02959.1"
                     /translation="MPHPLAALGERLSAQPARWLVTGSAGFIGSHLVETLLGWGQTVV
                     GVDNFATGHRRNLDEVRSLVSREQWRRHRFIEADICDLGACREACREVDFVLHQAALG
                     SVPRSLANPVATNAANVDGFLNMLVAARDAGVKRFVYAASSSTYGDHQALPKVEAHIG
                     RPLSPYAVTKLVNELYAEVFARCYAFPSIGLRYFNVFGARQDPEGAYAAVIPRWTRAM
                     LLGEEVTIHGDGETSRDFCFVDNAVQANLLAATVDDAAALNQVYNVAVDDRTSLNRLF
                     ELLRDELAEFRPDLRAVRPVYGDFRPGDVRHSQADIAKARRLLGYLPSHRLEAGIRAA
                     MPWYVSRFAVVEGGC"
     gene            complement(4990..>5667)
                     /locus_tag="PLL14_03205"
     CDS             complement(4990..>5667)
                     /locus_tag="PLL14_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOG02960.1"
                     /translation="AKVIENTQRDLNIALMNELAIIFDRIGIDTVEVLEAAGSKWNFL
                     PFRPGLVGGHCIGVDPYYLTHKAEMLGYHPEVINAGRRINDGMGKFIAEQTVKQLIRN
                     GWQVKDAPIIVLGLTFKEDCPDLRNSRVIDVIRELESYGARVLVHEPVADAGEAKHEY
                     GIEVQAWEQMPPAAAIVAAVAHRQFKERPLSDYVGKLQKGGVLTDVKSMFDEAQLVSH
                     GVTVWRL"
BASE COUNT         1100 a         1837 c         1670 g          960 t
ORIGIN      
        1 cgggccatgc gtgcaagatg ttcggcgctg acgaagacca tgctgtccgc aaacaggtgc
       61 ggtcgggttg cctgcagttg cgcgtgtgac agcgttccgt ccccggcttc gagcgcccgt
      121 ttcagcgatt cgtgatcgac ggagatgcac gcgcagccgc ggttgagcac ttcggccgtc
      181 gtccggcagt ccagcggcaa gcggtcggga acagattgaa gcaggctgtg catgcgtttc
      241 aggccgtcga gccaacggcg aagaaagcca gcgcttcggc cagctcgcgc gtccgcttca
      301 tcggcggcag gctgcgccag atccgcttgc cgtagggttt gctgaccaga cgcgggtcgc
      361 agatcatcag tacgccacgg tctgtctcgt cgcggatcag gcgacctgcc ccctgtttga
      421 cattgatcac ggcgcgcggc aactgatatt ccataaaggc attacgacct tggcccttga
      481 gccgctcgat gcgcgccgag agcacggggt cgtctggcgg agcgaacggc agtttgtcga
      541 tgaccaccag cgacagggct tcgccgcgca catcgactcc ctcccagaag ctctggctgc
      601 cgatcaggat ggcgttgccg agccggcgaa agcgctccag cagttcgttc ttgctgcgtt
      661 cgccctgcaa cagcacaggc agatcgagac tctcacgtgc cagccactcg acaagcaatg
      721 catgtgttct gcgcatcgcg cgcagcgagg tacagagaaa gaaggcccgg ccaccactcg
      781 ccttgagcac cggaaatgcg gccctgacga ccgcttcggc atagtccgcg ctgttcgggt
      841 cagaaagatt gcggggcacg taaagcaggg cctgctgcgc gtagttgaag ggactctccc
      901 aacgcgccga gcaagcaccg gtgagtccca tctcggcaca gaagtgcgca aaatcgtcct
      961 gcacggcaag tgtcgccgag gtgaatatcc aggcccgcgg gtgcccactc atctgtttct
     1021 gcatgatctc cgcaatgacc agaggcgtcg cgttgagctg tagcgaatgg gtgtgcgtct
     1081 caccccagcg aacgaaatcg ccattggatc cggcctgcca ggattgcagg cgggcgagca
     1141 actcggtcgc ccgccgccag cagctttcga ggccctcgga tcgctgcgcc tgtgtttcca
     1201 gcagagcagc gagaacttcc agttcgtcga tgacgcgcag catgcccggc gcgaaacccg
     1261 gccgcgcttc gagttgcaac agcgcgtagc gggccggctc caccggtagt gtcaagcgca
     1321 actcgcgcgc agccttttcc agcgcgccac acacgctggg cagatcgagg cagtcgcgcg
     1381 ccgacgcgcg cgcttccagt tgcgtatcgc gtgtcaattc gagaagctgc gccgtcgaca
     1441 ggctgtcgcc aaagaacaga ctggcaactt ccggcagttg gtgcgcttcg tcaaagatga
     1501 cggtattaca tgccggcaac agttccgccg taccttcatc gcgcagcatc acatcggcga
     1561 agaacaggtg atggttgacc accaccagat cggcagccag ggcctcacga cgagcggcca
     1621 ggacaaagca gtccttgtgc cgaggacagt cctggcccag acagttgtcc cgggtagagg
     1681 tgaccatcgc ccacaccgcc gcattctccg ggacgtcggc gcactccgcc ttgtcgccgc
     1741 tgtcggtctg tctggaaaaa cgtgcgatac gctgcgcgta ggcagcgtca tcgcgggaaa
     1801 gaaaacggcc gtccgccagc gaacgttcga ggtggtggtg gcagaggtaa ttggcccgtc
     1861 ccttgaggag agccaccttg accggcgact tgagcgcctt gcggatggtc ggaatgtcgc
     1921 gtgtgaagag ctggtcctgc agattcctgg taccggtcga aacgatcacc ttgccgccgg
     1981 aaagcagcgc cggcaccagg taggcgtagg tcttgccggt accggtaccg gcctcggcga
     2041 ccagaacgcg atagtcattc atcgccgcgg cgatgtgctc ggccaattca agctgctgcg
     2101 cccgcggacg ataaccggga atagcgcccg ccaggggacc atcggcagag aagacttcgc
     2161 agagcagagc ggtcatcgag gtttcgtcgg aagcaaagcg catgctaaca gaagctggcg
     2221 aagccaaggc ttccgccacc gccagcgggc gcaaataatc ccgccaaacg cagtcgtaac
     2281 ttcgcctgca ctaccgatga actcgcgcgc gcctcaaggg cctgcgcctg tcccgcagac
     2341 gcccgtgaaa agctcaaccc gaccttgctc gactacgggc ccttctgcat cagctcgcgc
     2401 aggaagcgca cgggaatggc aaaactgatt cccgagggct tgctgaggat cgactccttg
     2461 ttctccttga cgaacaccat gttgatgatg ccgatcactt cgccccggtt cacctcgtac
     2521 agcgggccac cactgttacc cgggtaagcc gtggcgtcaa gctgatagat atcgaagctg
     2581 ccgctcctga tctgctggac gaccctcgcg ttgagctggc gtgcattcgc cccgggcagg
     2641 acgatcggcg ttatcgacga gatgatgccg cgatgcgtga ccggcgacag gcccagcacg
     2701 ccgccgatcg gaaagccggt aaacgccacc gactgcccct ctctgaccgg ttccgaatca
     2761 tgcagggcca gcgccggcag ggcctggcca tcgagccgca acagggccag atcatgcgcc
     2821 ttgtcgactg caaaatccct gacccggcgt tgctgcgtcg ccgctgcgga caaacgcgcc
     2881 accacgacca gcatctctcc cgcattcaga tccagcggct cctggatcac gtgcgcattg
     2941 gtcgccacct cgcgaccgtc gccaacgacg aaacccgtac cccgcatcac gaactgtggg
     3001 ctgcgcgtga gctgataggt gccaacgaca accacnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnncgcgt ctcggcatag gggggtactc cgcggtaacg attcaccgct
     3181 ccctcaccgg cgttataggc ggcggccacc agactgacgt cgccttcgaa ataggccagc
     3241 agccaacgca gataggccag accaccacgc aggttctgct ccgggtcgaa aggcttcttg
     3301 acattgaaac gcgccgacgt atccgggatc aactgcatca ggccctgagc attcttgttc
     3361 gacagggcca tcgggttgaa attggactcg gcccggatca ccgccagagc cagacgcgga
     3421 ctgactccgt actccggcgc cagttggttg accagaccga ggaccttctt ctgagcttcg
     3481 ctgctcggca acatgacatc cggcgactcg tcgacaaaga ccacggcgcg catgcactcc
     3541 ggcaccgctg atccgggttc gccgacaaaa cgctgcatcc tctgcgacgg cgcatgcccc
     3601 tgcttcgccg ccaggctaaa gaaatacgcg gcccgcgcat cgtcccgttc gataccgcgc
     3661 ccgttggcgt acatccaccc gagactgaat tgcgcctcgg ggtcgccaag acgagcacca
     3721 tcgcaataca gcttggccgc ccgggcgtaa tccctgggca caccctcgcc atgctcgtgg
     3781 gccacggctt ccttgcgcaa ggccagcaga cgctcggcat ccgcttccct gggaatctcc
     3841 ccggcgatca gcatcgacgc gctcagcagc ccgtaacaga ccacgccccc ccggagcagc
     3901 cgtgccgcag agtgaataaa cgaacgctcc atcaacagcc tccctcgacc acggcgaacc
     3961 gtgacacgta ccacggcatc gcagctctga ttccggcttc gagccggtgg ctcggcagat
     4021 atccgagcaa gcggcgggct ttcgcgatat cggcctgcga gtgccgcacg tcgcctggcc
     4081 gaaaatctcc gtacaccggc cgcacggcac gcaggtccgg ccggaactcg gcaagctcgt
     4141 cgcgcagaag ttcgaagagc cggttcagtg aggtccggtc atcgaccgcg acgttgtaca
     4201 cctgattgag tgccgccgca tcgtcgacgg tcgctgccag caggttggct tgtacggcgt
     4261 tgtcgacgaa gcagaaatca cggcttgttt caccgtcgcc gtggatggtc acttcctcgc
     4321 cgagcaacat cgcccgggtc cagcggggaa tcaccgcggc gtaagcaccc tcggggtcct
     4381 ggcgggcacc gaacacattg aaatagcgca aaccgatcga cggaaaggca taacaacgcg
     4441 cgaatacctc ggcgtagagt tcattcacca gtttggtcac ggcatacggc gagaggggcc
     4501 tgccaatgtg agcttcgacc ttgggcagtg cctgatgatc accataggtc gaactcgatg
     4561 cggcgtaaac gaaacgcttt acgccagcgt cgcgtgccgc gacaagcatg ttgagaaaac
     4621 cgtcgacgtt ggccgcgttg gtggccaccg gattggccag ggagcgcgga acggaaccca
     4681 gcgccgcctg gtgcaggacg aaatcgacct cgcggcaagc ctcgcgacac gcccccagat
     4741 cgcagatatc ggcctcgatg aaacggtgac gacgccactg ctcgcgactc accaatgatc
     4801 gcacctcatc gagattccgc cgatggccgg tggcaaagtt gtccacaccg acaaccgtct
     4861 gcccccaccc cagcaaggtc tccacgagat gcgaaccgat gaagccggcg ctgccggtga
     4921 ccagccagcg tgccggctgc gccgacaacc gctcgccgag cgccgccaga gggtggggca
     4981 atgcggaact caaagacgcc acaccgtcac tccatgcgag accagttgcg cctcgtcgaa
     5041 catgctcttc acgtcggtca gaacgccccc cttctgcagt ttgccgacgt aatcgctcaa
     5101 gggccgttcc ttgaactggc gatgggcaac ggcagcgaca attgccgctg ccggtggcat
     5161 ctgctcccag gcctgcactt cgatcccgta ttcatgcttg gcctcgcccg catcggcaac
     5221 cggttcatgc accagcaccc tcgcgccata ggactcgagt tcccgaatca cgtcgatgac
     5281 ccgcgaatta cgcaggtccg ggcagtcttc cttgaaagtc aggccgagca cgatgatcgg
     5341 cgcatccttg acttgccagc cattgcgaat cagttgcttg acggtctgct cggcgatgaa
     5401 cttgcccatc ccgtcgttga tccggcgtcc ggcgttgatc acctccgggt ggtaacccag
     5461 catctctgcc ttgtgcgtca ggtaatacgg atccacaccg atacagtgcc caccaaccag
     5521 gccgggccgg aaggggagga agttccactt gctgccggct gcttccagga cctcgacggt
     5581 gtcgatgccg atgcgatcga agataatggc cagttcgttc atcagggcga tgttcaggtc
     5641 gcgctgggtg ttctcgatga ccttggc
//
LOCUS       DAOSIT010000082         5648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12876_length_5648_cov_8.565886, whole genome shotgun sequence.
ACCESSION   DAOSIT010000082 DAOSIT010000000
VERSION     DAOSIT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12876_length_5648_cov_8.565886"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..673
                     /locus_tag="PLL14_03210"
     CDS             23..673
                     /locus_tag="PLL14_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOG02961.1"
                     /translation="MDDNQAEDKPIFRRLYERVFPTVADFFGMLAEQSANAAETTRLL
                     VEFMETGDVSVSQKVRDDEHEADRIKAANLSVLSDAFSTPVDREDLFRAITTLDDIVN
                     YCKSTVVEMDMLGLQPDKHSLQIALHIREGTDALAKGFARLATDPAGAGLDANAARKA
                     ERTVEKSYRRAITELFQGDDYLNMFKRRETYRHLSNAADRVADAARALDNIVVKLC"
     gene            760..891
                     /locus_tag="PLL14_03215"
     CDS             760..891
                     /locus_tag="PLL14_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02962.1"
                     /translation="MGKLIAGLMALALALSSAHAVACSAEKAKDGQTEMSTPAKPKS"
     gene            1117..>1329
                     /locus_tag="PLL14_03220"
     CDS             1117..>1329
                     /locus_tag="PLL14_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768141.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HOG02963.1"
                     /translation="MAQLVILPGKDRSLVRRHPWIFATAVERLSGRARPGDTVDVVAA
                     NGRPLAKAAWSPESKIRARVWTFDPEE"
     assembly_gap    1330..1429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1430..2182
                     /locus_tag="PLL14_03225"
     CDS             <1430..2182
                     /locus_tag="PLL14_03225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOG02964.1"
                     /translation="SACARIYERSDSGVRRLEGLEPVTGWVFGEASSDPLAIVENGVR
                     LRVDIVAGHKTGFYLDQRDNRVLTRVLAAGRATLNCFCYSGAFSLQALAGGASSVLSI
                     DSSGPALAMAAENLALNPHLDAHRAEWREADVFNELRALNDQGRRFDLIILDPPKFAP
                     SAVHAERAARAYRDINVFGFRLLNPGGLLMSYSCSGGISGEHFQQILAEAALGAGREA
                     RILRRLGASPDHPVALNFSEGEYLKGLLCQVE"
     gene            complement(2204..2812)
                     /gene="msrA"
                     /locus_tag="PLL14_03230"
     CDS             complement(2204..2812)
                     /gene="msrA"
                     /locus_tag="PLL14_03230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOG02965.1"
                     /translation="MSTHLRLAAMLLLIGGHAGGAEPPAPRATEVRTAIFAGGCFWCI
                     EADFEKLPGVLAAESGYTGGRTANPSYEQVSAGTTGHTEAIRVSYDPQRVSYPQLLDY
                     FWRHIDPTVRNRQFCDVGTQYRSGIYWQNEAERKAAESSRDALLASGKLPHIETEIAA
                     ATTFYPAEEYHQDYYKKNPIRYAYYRQGCGRDARVKQIWGSS"
     gene            complement(2809..3315)
                     /gene="msrB"
                     /locus_tag="PLL14_03235"
     CDS             complement(2809..3315)
                     /gene="msrB"
                     /locus_tag="PLL14_03235"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOG02966.1"
                     /translation="MDRRQSLKWLSGLSAFVLPVTAPGPAWAGNGSKLQKSKTEWAAL
                     LPQPAYRVLFEEDTERAGSSPLNQEKRDGTFICAACHLPLFDSATKYDSGTGWPSFWE
                     HLPESIATRTDFKLFYPRTEYHCSRCGGHQGHVFNDGPKPTGKRYCNNGLALQFVPRS
                     EKLPELRT"
     gene            3781..4179
                     /locus_tag="PLL14_03240"
     CDS             3781..4179
                     /locus_tag="PLL14_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOG02967.1"
                     /translation="MLIRGTGARATPARVRVLRLLRMAPAALTHHEIEQAIGDLPVDR
                     VTLYRVLDWLVESGLAHRNADAQRVFRFSAAAIGEHSAHTHFRCEDCGRVFCLDSAPP
                     APPSLPEGFSLSHLELDLRGRCAGCTGEHR"
     gene            4320..5381
                     /locus_tag="PLL14_03245"
     CDS             4320..5381
                     /locus_tag="PLL14_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG02968.1"
                     /translation="MRVPDPERTIPVTVLTGFLGAGKTTLLNHLLRQPALANAAVLIN
                     ELGDVAVDHHLVDRVDDNVVILGSGCLCCSVRGEMARSLRDLFMRRLRREIPAFARVL
                     IETTGLADPAPVLYTLLEDFFIAERFRMDGVVTVVDVTHAGRQLSRHFEAIRQVAMAD
                     RLLLTKCDLATPEEIAGVARRLARANPGAPQIEVYRGVVCAEQVMGCGLYDPGSKTAD
                     VRRWLAEEKVAAAAVLDQLAQVPAGNRHDALVHAFVLRFEQPFAWPEFAEAIDVLLAT
                     CGDRILRVKGLIAVQGENGPRVLQCVQHMRYPEAMLPAWPDGDHQSRLVFIVRALAQD
                     IVSQAFAMFCGVSAQPCSR"
BASE COUNT          967 a         1701 c         1779 g         1101 t
ORIGIN      
        1 acactaactg aggagatgaa ccatggacga caatcaagcc gaagacaagc caatttttcg
       61 ccgtctgtat gagcgggtct tcccgaccgt agcggacttt ttcggcatgc tcgccgagca
      121 gagcgcgaac gccgcggaaa ccacccgtct gctcgtggaa ttcatggaga ccggcgacgt
      181 gtcggtcagc cagaaggtac gcgacgacga gcacgaagct gaccggatca aggccgccaa
      241 cctcagtgtg ttgagcgacg ccttctcgac accggtcgac cgcgaagacc tcttccgcgc
      301 gatcaccacg ctcgacgaca tcgtgaacta ctgcaagtcc accgtcgtcg agatggacat
      361 gctgggtctg caacccgaca agcacagcct gcagatcgcg ctgcatattc gcgaaggcac
      421 ggacgcgctg gcgaagggct ttgcgcgcct ggcgactgac ccggcgggtg ctggtcttga
      481 tgccaatgcc gcacggaagg ccgagcgcac tgtcgaaaag tcttatcgac gggcgattac
      541 ggaactgttc cagggtgacg actacctgaa catgttcaag cgccgcgaga cctatcgcca
      601 cctgtcgaac gcggctgacc gcgtcgccga tgcggcgcgg gcgctggaca acatcgttgt
      661 caagctgtgc tgaaagccac tcgcgcaatc agggttgtat ttcgcaaccg ttcggcctag
      721 agtggcctcg ccaatatcgg cagccacaag gagaagcgta tggggaaact gatcgccggc
      781 ctgatggcct tggctctggc gctatcaagt gcacatgctg tcgcctgttc ggctgaaaaa
      841 gccaaggatg gccagacgga aatgagcacc ccggccaaac caaaaagctg acttgtcggt
      901 gtttgcagaa cgcgcggcgc ggtacctctg gtatcgcgcc gcgtcacgtt tcgggcgtgt
      961 attcgtgcct gcgctttcgt tgatgagcct caaaaaaatg gggggccggc agcaaactcc
     1021 caaccccgga ttcggcgaga cacccgcagt ggtgtagact gcgccgctcc aacctcattg
     1081 tcgcccggct ggcatgccag gcgcgggata ctgtgtatgg ctcagctcgt tattctgcct
     1141 ggcaaggatc gctcactggt ccgtcgtcac ccctggattt tcgccactgc ggtcgaacga
     1201 ctgagcggtc gtgcgcggcc gggcgatact gtcgacgtcg ttgccgccaa cggccggccc
     1261 ctggcgaagg cggcgtggag tcccgagtcg aagatcaggg ccagggtgtg gaccttcgac
     1321 cccgaggaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcgcttgcgc
     1441 acgcatctac gagcgatcgg attcaggcgt tcgccggctg gaggggcttg agccggtcac
     1501 cggttgggtg tttggcgaag cttcgtcaga tccgctggcc atcgtcgaaa acggcgtgcg
     1561 cctgcgtgtc gacatcgttg ctggccacaa gacgggtttt taccttgatc aacgcgacaa
     1621 ccgggtgctg acacgggtcc tcgcagccgg tcgtgcgaca ctcaactgct tctgctatag
     1681 cggcgccttt tcgctgcagg cgctggctgg cggtgcgagc agcgtgttgt cgatcgactc
     1741 gtccggcccc gcgctggcta tggcggcaga aaatctggcg ctcaacccgc atctcgacgc
     1801 gcaccgtgcc gaatggcgag aggcggacgt tttcaatgaa cttcgggcgt tgaatgatca
     1861 ggggcgacgc ttcgacctga tcattctcga tccgccaaag ttcgctcctt cggccgtaca
     1921 cgccgaacgc gctgcgcggg cctatcgcga catcaacgtc ttcgggtttc gcctgctgaa
     1981 tcccggtggt ctgctgatga gttattcgtg ctcgggcggc atcagcggtg aacactttca
     2041 gcagatcctt gccgaagccg cgcttggtgc cgggcgcgag gcgcgtatcc tgcgccgtct
     2101 cggggcctct ccggaccacc cggtggcctt gaatttcagt gaaggtgaat acctcaaggg
     2161 tctgctctgc caggtggaat gagtacgcgg cgagccaggg cgactagctg ctcccccaga
     2221 tctgctttac ccgggcgtcg cggccgcaac cctggcggta ataggcataa cggatcgggt
     2281 tcttcttgta gtagtcctgg tggtactcct ctgccgggta gaaggtggtg gcggcggcga
     2341 tctcggtttc gatatgcggc agcttgccgc tggccagcaa ggcgtcacgg ctactttccg
     2401 ccgccttgcg ttcggcctcg ttctgccagt agatgccgct gcggtattgt gtgccgacat
     2461 cgcagaattg tcggttcctg actgtcggat cgatatggcg ccagaagtaa tcgagcaact
     2521 gcggatagct cacccgctgc ggatcgtagg acacgcgtat ggcttcggta tgtccggtgg
     2581 tgccggcgct gacctgctca taacttgggt tcgccgtacg gccgccggta tagccagact
     2641 ctgcggcaag aacccccggc agcttttcga aatccgcttc gatgcaccag aagcagccgc
     2701 cggcgaaaat tgctgttcga acttcggttg ctctgggtgc gggtggctcg gcaccaccgg
     2761 catggccgcc gatcaggagc agcatcgccg ccagtcgcaa gtgcgtgctc atgtccgcaa
     2821 ctccggcagc ttctccgagc gcggcacgaa ttgcagggcc aggccattat tgcaatagcg
     2881 cttgccggtg ggcttggggc catcgttgaa gacatggcct tggtgtccgc cgcagcggga
     2941 gcagtggtac tcggtacgcg gatagaacag cttgaagtcg gtcctggtgg caatcgactc
     3001 cggcaggtgt tcccagaagc tgggccagcc ggtgccgctg tcgtacttgg tcgcgctgtc
     3061 gaacagtggc aggtggcagg cggcgcaaat gaaggtgccg tcgcgctttt cctggttcaa
     3121 tgggctgctg ccggcgcgtt cggtgtcctc ttcaaagaga acgcgatagg ccggctgtgg
     3181 cagcagggcg gcccattcgg tcttcgattt ctgcaacttg ctgccgttac cggcccaggc
     3241 tgggcccggg gcagtaacgg gcaggacgaa ggctgacaag ccgctcagcc atttcagtga
     3301 ttgacgacga tccatgagat atctccagta acaactaagg gacgccagtc cggcgcagca
     3361 ggctgccggt ggctggggcg atcaacccca acagattggc tgcctgcagt ccgaaacaaa
     3421 atgtggccct gcgaaacaga cgtaccatct gctgtggttc tgggcatcga cgtgtacaga
     3481 cagcgctttc aggtgttcgg ccgaaaagca ctggtacagt tccccaagcc agcaaccggg
     3541 aggctgacaa cagctgcagt tctcgccggc gcggacacga gtgatgaccg tttgcgctat
     3601 ggtcgttcaa gcaaccgtca accttacgcc tcgtgtgtgc agccgcgttg tttaattgcc
     3661 cctgctgcga cgcccatccg gcgcaactct gttgcatctg gcatcgtcct gtgcttaaat
     3721 cgttgcatga ctgtggtcaa gaatactgtc gaatccgccg cggaggctga aactgctgcc
     3781 ttgctcattc gcggcacggg tgcgcgcgcg acgccggcgc gggtgcgcgt cctgcggctg
     3841 ctgcgtatgg caccagcggc gctgacgcac cacgagatcg agcaggcgat cggtgatctg
     3901 cccgtcgatc gggtgacgct ctatcgcgta ctcgactggc tggtggagag tgggctggcg
     3961 caccgaaatg ccgatgcgca gcgggtcttc cgcttctcgg cggctgccat cggcgagcat
     4021 tcggcgcaca cgcattttcg ttgcgaggac tgcgggcgtg tgttctgtct cgattcggcg
     4081 ccgccagctc cccccagctt gcccgagggt ttttcgctgt cacatctcga actcgacctg
     4141 cgcggtcgat gcgccggctg taccggcgag caccgatgaa cggcgatcac cgccgggcgc
     4201 ctgcaatcag ctcgacaagt tcatggcggt ccgttacgcg caggggaagc gaagcggccg
     4261 caacgtacgc gccagcggta gggtgcggcg cgcgaaaccg cgcgcgcgag gcctgagcca
     4321 tgcgtgttcc agaccccgag cggaccattc cggtgaccgt ccttaccggt tttctggggg
     4381 ccggcaagac cacgctgctc aaccacctgc tgcgacagcc agcactcgcc aatgccgccg
     4441 tcctgatcaa cgaattgggc gacgtcgccg tagaccacca cctggtcgac cgggtggacg
     4501 acaatgttgt cattctcggc tcgggttgtc tgtgctgcag cgtacgcggg gagatggcgc
     4561 gcagcctgcg cgacctgttc atgcgtcgct tgcgtcgcga gatccctgcg ttcgcgcgcg
     4621 tactcatcga aaccactggc ctggccgatc cggcgccggt gctctacacc ctgctcgaag
     4681 atttcttcat tgccgaacgc tttcgcatgg acggggtggt cacggtggtc gatgtcactc
     4741 atgccggtcg ccagctcagt cgtcacttcg aggccatacg ccaggtggcg atggcggacc
     4801 gtctgctgct caccaagtgt gacttggcga cccccgaaga gattgccggc gttgcccgcc
     4861 gtctggcacg cgccaatcct ggtgcgccac agatcgaggt ctatcggggt gtcgtctgcg
     4921 cagagcaggt gatggggtgt gggctatatg atccgggcag caagacagcc gatgtacgcc
     4981 gctggctggc cgaggagaaa gtggctgccg ccgctgtgct cgatcagcta gcgcaagtgc
     5041 ctgccggtaa ccgccacgat gcgctggtgc atgccttcgt gctgcgtttc gagcagcctt
     5101 ttgcttggcc ggagttcgcc gaggcgatag acgttctgct cgccacctgt ggcgatcgca
     5161 tcctgcgcgt caagggcctg attgcggtgc agggtgaaaa cgggccacgc gtgctgcagt
     5221 gcgtgcagca catgcgctac ccggaagcga tgctgcctgc ctggccggac ggcgaccacc
     5281 agagccgtct ggtgttcatc gtgcgtgccc tggcacagga catcgtgagc caggccttcg
     5341 ccatgttctg cggtgtgtcg gcgcagccgt gcagccgctg actgtatcgc gaacttccat
     5401 gtgacgcggg gctcgttggc ggtcggcaga atcgacaaag cccaccgcca aggtcgagga
     5461 agacgctaga attccccact gttgcggcca gccctttttc taccgatgtt tccctccgag
     5521 ttcacgctga gtctcgttcg tcatccggca acgcccgccc cggtggtgcg ctcacttgaa
     5581 gcgcacgctg cgtacgcggc cgatggcggt cttgaaatcg cctactgtct gcgcggcgac
     5641 atggcccg
//
LOCUS       DAOSIT010000083         5600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_12999_length_5600_cov_7.646889, whole genome shotgun sequence.
ACCESSION   DAOSIT010000083 DAOSIT010000000
VERSION     DAOSIT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12999_length_5600_cov_7.646889"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PLL14_03250"
     CDS             <1..1140
                     /locus_tag="PLL14_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOG02969.1"
                     /translation="AANFRRLVVDIGGGSTEFIIGERTEPLLMESLFMGGISYRQRFF
                     PEGKVDKKRFYAAEVSAAREIEAIAVDYQRFGWQEAVGSSGAAQEIANILEMNDLNPD
                     GGSGISREGLARLRQLIIRAGSAEALDLKGMRGDRLVILPGAIAIMSAVFSELGMAHM
                     TYSDGALPLGVLYDLLGRFEHHDTRDETVAQFMRRYQVDETQVRRVERTALLLLGQMI
                     KLDSPEHENHVHFLRWAVSLHEIGVSVAHSEFHKHGAYILAHADMPGFSKRDQARLAL
                     LVLGQHGKLQKLASMPAGDPNWRLVFCLRLAALLHRSREDQPVSQVSVKESPVGFQVE
                     VPAVWLDDNPMTAAALTDEALLWRRIGLKVRVRPMPSEAAVPVEG"
     gene            1335..2447
                     /locus_tag="PLL14_03255"
     CDS             1335..2447
                     /locus_tag="PLL14_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02970.1"
                     /translation="MTVATIETVRQADGAQVRLSGDWTLVTLPPPIAGLELRLRDLAS
                     GNTCWDLQPIERMDSAGAILLWRAWGRQWPASLAVSGAHRSLLERVEAIRADVEPEQP
                     SASWTWIRLLGGGALAAIGHLAAMLALIGQMLLDVAHLCVHPGDIPWREFSANLYKSG
                     AQALPVTALIGFLIGIVLSYLSALQLATFGADVFIVNLLGISIVRELGPVLVAVLVAG
                     RSGSAMTAQIGVMRVTEEIDALATMGVSRSLRLVLPKVLALALAMPLLVIWCSAAGIV
                     GGMVAAKLQMDLSFGFFIDTLPKVVPVANVWIGLGKGAVFGVLIALIACHFGLRVKPN
                     TESLSSRTTTSVVTSITVAILVDAVFAVLTRGVGLP"
     gene            2447..3244
                     /locus_tag="PLL14_03260"
     CDS             2447..3244
                     /locus_tag="PLL14_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG02971.1"
                     /translation="MSARSDSGRQTPLAIEIRGLCTQFGDVVIHRDIDLDVEAGQMLG
                     LVGGSGSGKTTLLREIVGLLAPSKGSVKLFGQAVLDPDPELRRQLRRRFGMLFQQGAL
                     FSALSVFDNIAFPLRELRLLDEGMIADLVLLKLGMVELEARHGRLMPAELSGGMVKRV
                     ALARALSLEPELLVLDEPTAGLDPDRSENFVNLIKMLQKALGFTVVMVTHDPETLAGL
                     ATRLAVLADQRIVACGTAAEVCAVEHPFIRNFFSSGRAAATVRQGAC"
     assembly_gap    3369..3468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3633..4256
                     /locus_tag="PLL14_03265"
     CDS             3633..4256
                     /locus_tag="PLL14_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOG02972.1"
                     /translation="MKSLLMCLAVCCLAACAGSARNASLIEAYDFGLPPRHLPDDDGR
                     WSSVALEVSVPSWFDSPGIEYRLLYENPLKLRSYAGSRWVGAPGLLLSQRLRQQLGFV
                     GVSGHGAVTCLLRLELQEFSQVFDTPEHSRALLQGRASLLDRGRRGVVDRRVDIEQAA
                     TTPDARGGVLALVSVSDELGRQLAAWLNDLQKRGTLNGCRVTAAHDQ"
     gene            4286..>5600
                     /locus_tag="PLL14_03270"
     CDS             4286..>5600
                     /locus_tag="PLL14_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG02973.1"
                     /translation="MQNIYQQGLDKNTANYTPLTPLTFIARTAYIYPDRTAVIHGQRR
                     YTWGETFRRARRLASALVDHGVGVGDTVAAMLNNTPEMVECHFGVPATGAVLNTLNTR
                     LDAETIAFMLEHGEARVLITDREYSPTVRKALAATQRQILVIDVDDSEYSGPGQRLGT
                     QEYESFIAAGSPDFPWGGPSDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNSMSNIVS
                     WGMAPHSVYLWTLPMFHCNGWCFVWTMAANAGTNVCLRRVDPKLIFEAIREHRVTHYC
                     GAPIVHSLMANAPAELRAGISQKVAGLIAAAPPPAAVIEQMANIGVDLTHVYGLTETY
                     GPAAVCAKHSHWADLPLAEQVVLNGRQGVRYHAQEAITVLDSASMEQVPWDKETMGEI
                     MFRGNLVMKGYLKNPKATEESFAGGWYHTGDLAVMHPDGYVKIKDR"
BASE COUNT          911 a         1674 c         1812 g         1103 t
ORIGIN      
        1 gcggccaatt tccggcgtct ggtggtcgat atcggcggcg gttcgaccga gttcatcatc
       61 ggagagcgta ccgaaccgtt gttgatggag tcgctgttca tgggcggcat cagctatcgg
      121 cagcgctttt ttccagaagg caaggtcgac aagaagcgct tttatgcagc ggaagtgtcg
      181 gccgcgcgcg aaattgaagc gattgccgtc gactaccagc gcttcggttg gcaggaggcc
      241 gtgggttcct caggcgcggc gcaggaaatc gccaacattc tcgaaatgaa cgacctgaat
      301 cccgatggcg gcagcggcat cagtcgggag ggcctggcca ggctacgcca gttgatcatc
      361 cgtgccgggt cagcggaggc gctggatctc aaaggcatgc gcggcgaccg cctggtgatc
      421 cttcccggtg ccattgccat catgtcggcg gtgttctccg agttggggat ggcgcatatg
      481 acgtattccg acggtgcgct gcccctgggg gtcctctacg atctgcttgg gcgcttcgaa
      541 caccacgaca cgcgcgacga aacggtggcc cagttcatgc gtcgctatca ggtcgatgag
      601 acgcaagtga ggcgcgtcga gcgcaccgcg ctgttgcttc tcggtcagat gatcaagctc
      661 gattcacccg agcacgaaaa ccacgtccat tttctgcgct gggcggtctc cctgcatgaa
      721 atcggtgttt cagtggcgca cagtgaattc cacaaacacg gcgcctatat tctggcgcat
      781 gccgacatgc ccggcttttc gaagcgcgat caggcccgtc tggcgttgct ggtgctcggc
      841 cagcacggca agctccagaa actggcttcg atgccggcag gcgatccgaa ctggcgcctg
      901 gtgttctgtc ttcgcctggc agcccttctg catcgctcgc gcgaggacca gccggtgtcg
      961 caagtgtcag tcaaggaatc gcccgtcggt ttccaggtgg aggtgccggc cgtctggctg
     1021 gacgataacc ccatgactgc cgcggcgttg accgatgagg cgctgctctg gcgtcggatt
     1081 ggcctcaagg tgcgggtcag gccgatgccc agcgaagcgg cggtgcccgt tgaggggtga
     1141 acagcgtgtc acaggctggc ggcgaccgtc tcgccctgtc gctcgtcggc cgggatcgcg
     1201 gtgcgcacgc agcctgagcg ggcctgtggc ggcgagccac acagcgtacc gactggaccg
     1261 gcctggagtc agcgaatgag tggcaaggca gggcagtcgc gctgcggttg tccgcgagga
     1321 tcctgagacc cgcgatgacg gtggcgacca tcgaaacagt gcgtcaggcc gacggggccc
     1381 aggtccgtct ttccggcgac tggaccttgg tcaccctgcc gccgccgatt gccgggctcg
     1441 agctacgtct gcgcgatctg gcgtccggca atacgtgctg ggatctgcag ccgatcgagc
     1501 gcatggacag cgccggtgcg atcctgctct ggcgtgcctg gggtaggcaa tggccggcct
     1561 cgctggcggt ttccggtgcg caccgatcgc tgctcgaacg tgtcgaggcc atccgtgccg
     1621 acgtcgaacc ggagcagccg agcgcatcct ggacctggat tcgcctgctt ggcggcgggg
     1681 cgctggcggc catcggccat cttgccgcca tgctcgccct gattggccaa atgctcctgg
     1741 acgtcgcgca tctgtgcgtg catccgggtg acattccctg gcgcgagttc tcggccaatc
     1801 tttacaagag cggtgcgcag gccttgccgg tgacggcatt gatcggtttt ctgatcggca
     1861 tcgttctctc ctacctgtcc gctctgcaac tggcgacctt tggtgccgac gtcttcatcg
     1921 tcaacctgct cggcatcagc atcgtccgcg aactcggccc ggtactggta gcggtgctgg
     1981 tcgcaggacg ttcggggtcg gcgatgaccg cccagatcgg cgtcatgcgg gtaaccgaag
     2041 aaatcgacgc gctggccacg atgggtgttt cccggagtct gcgcctggtg ttgccgaagg
     2101 tcctggcgct ggccctggcg atgccgttgc tggtcatctg gtgttcggca gccgggatcg
     2161 tcggcggcat ggtcgcggcc aaactgcaga tggatctgtc ttttggcttc ttcatcgata
     2221 cgttgcccaa ggtggtcccg gtcgccaatg tctggatcgg cctgggcaag ggcgctgtct
     2281 tcggcgtgct gatcgcgctt atcgcctgcc actttggcct cagggtcaag ccgaataccg
     2341 agagtctttc gtcgcggacg accacgtcgg tggtgacttc gatcacggtg gcaattctgg
     2401 tcgatgccgt cttcgcggtg ctgacccgtg gtgtggggct tccctgatga gcgcacgctc
     2461 ggacagtggc cggcaaacac cgctggcgat cgaaatccgt ggcctgtgca cgcagtttgg
     2521 cgatgtcgtc atccaccgcg acatcgatct cgatgtcgaa gccgggcaga tgctgggtct
     2581 ggtcggcggt tcgggcagcg gcaagacgac cctgctgcgc gagattgtcg gcctgctggc
     2641 gccgagcaag gggtctgtca aactcttcgg gcaggccgtg ctcgatccgg atccggaact
     2701 gcgtcgccag cttcgccgcc gcttcggcat gctctttcag cagggggcgc ttttttcggc
     2761 gctctcggtt tttgacaata tcgccttccc gttgcgtgag ctacgcctgc tcgacgaggg
     2821 catgattgct gatctggtgc tgctcaagct ggggatggtc gagctggagg cgcgtcacgg
     2881 ccgcctgatg ccggccgagc tctccggcgg gatggtcaag cgtgttgctc tggcacgagc
     2941 cctgtcgctg gagcccgagc tgctggtgct cgacgagccc accgccggcc tcgacccgga
     3001 tcgcagcgaa aacttcgtca acctgatcaa gatgctgcag aaggcccttg gttttaccgt
     3061 ggtgatggtg acgcacgacc ccgaaacgct ggccgggctg gcaacccgcc tggccgtgct
     3121 tgccgatcag cgcatcgtcg cctgcggtac ggccgcagaa gtgtgtgccg tcgagcaccc
     3181 gttcattcgc aatttcttta gttccggacg cgctgcagcg accgtcaggc aaggggcctg
     3241 ttaagcatgg agaaccgttc gcacgccctg ctggcagggc ttttcacgct ctttctcggt
     3301 cttgcggtgg tgtgctcgat ctggtggttt ggcggcaagc acgaagcgac gacggactac
     3361 acggtggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt caatgaagtg
     3481 ggcagcggtc tggcgcgcaa ctcgcgccag cttgacaggg tattgcaggt gctcgaagaa
     3541 tcgccgcaga gcctcctttt cgggccggta tcgccgctcc cgggacctgg cgaggccggc
     3601 tttgttgttc cgtcaagtcg caaggggtca ccatgaagag cttgctgatg tgtctggctg
     3661 tctgctgtct cgcggcgtgc gccggcagcg ctcgcaacgc gtcgctcatc gaagcctacg
     3721 acttcgggct gccaccccga cacctgcccg acgatgacgg gcgctggtcg agtgtggccc
     3781 tggaggtgag tgtgccgtca tggttcgact caccgggaat cgagtatcgt ctgctctacg
     3841 agaacccgct caagctgcgc agctacgcgg gcagtcgttg ggtgggagcg ccgggattgc
     3901 tgctgtcgca gcgcctgcgg caacagcttg ggttcgttgg cgtcagcgga catggcgcgg
     3961 tgacttgcct gttgcgcctc gaactgcagg agttctcgca ggtgttcgat acgcccgagc
     4021 atagccgcgc cttgctgcag ggtcgggcca gcctgcttga cagagggcgc cggggagtcg
     4081 ttgaccggcg ggttgacatc gagcaggcgg ccacgacgcc agacgctcgt ggtggcgtct
     4141 tggcactggt ttccgtcagc gacgaactcg ggcggcagct cgctgcctgg ttgaacgatc
     4201 tgcagaaacg tggcacgcta aatggctgcc gggtaacggc ggcgcacgac caatgacaat
     4261 tgacactctt tcgaggggga gctgaatgca gaatatttat caacagggtc tcgacaagaa
     4321 cacggccaac tacacgccac tgacgccgct gaccttcatt gcccgcaccg cttacatcta
     4381 tccggatcgc accgcggtga ttcacgggca gcgccggtat acctgggggg aaaccttccg
     4441 acgcgcccgt cgcctcgctt cggcactggt ggaccacggc gttggcgtcg gcgataccgt
     4501 ggcggcgatg ctcaacaata cccccgagat ggtcgaatgc cattttggcg tgccggctac
     4561 cggtgccgtc ctgaatacac tcaacacgcg tctggatgcc gaaacgattg ctttcatgct
     4621 cgaacatggc gaagccaggg tgctgattac cgaccgcgaa tattcgccca ccgtcaggaa
     4681 ggcgctggcc gctacccagc gccagattct ggtcatcgac gtcgacgact ccgaatacag
     4741 cggccctggc caacgcctgg gcacgcagga gtacgagtcg ttcatcgccg ctggcagccc
     4801 ggactttccg tggggcggtc cgagcgacga atgggatgcc atctcgctca attacacctc
     4861 gggaactacc ggcaatccga agggcgtggt ctatcaccat cgcggcgcct acctcaactc
     4921 gatgagcaac atcgtcagtt ggggcatggc tccgcattcg gtatacctgt ggacgttgcc
     4981 catgtttcac tgcaacggct ggtgcttcgt gtggaccatg gccgccaacg cgggaaccaa
     5041 cgtctgtctg cgccgggtcg atccgaaact gattttcgag gccatccgcg agcaccgggt
     5101 cacccactac tgcggcgcgc cgatcgtgca cagcctgatg gccaacgctc ccgccgagtt
     5161 gcgtgcggga atctcgcaga aggttgccgg cctgatcgcc gccgctcctc cgccggcggc
     5221 ggtcatcgaa cagatggcga atatcggcgt cgatctcacg catgtgtacg gactgaccga
     5281 aacctatgga ccggcagccg tttgtgccaa gcacagccac tgggccgatc tgcctctcgc
     5341 cgagcaggtc gtgctgaacg gcaggcaggg cgtgcgttac cacgcacagg aagcaatcac
     5401 cgtgctcgat tcggcgagca tggagcaggt gccctgggat aaagagacga tgggcgagat
     5461 catgttccgc ggcaatctgg tcatgaaagg ctatctcaag aatccgaagg cgaccgagga
     5521 gtcgtttgcc ggtggctggt accacaccgg cgatcttgcg gtcatgcatc cggatggcta
     5581 cgtgaagatc aaggatcgct
//
LOCUS       DAOSIT010000084         5579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13063_length_5579_cov_7.911839, whole genome shotgun sequence.
ACCESSION   DAOSIT010000084 DAOSIT010000000
VERSION     DAOSIT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13063_length_5579_cov_7.911839"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..4255)
                     /gene="rpoC"
                     /locus_tag="PLL14_03275"
     CDS             complement(44..4255)
                     /gene="rpoC"
                     /locus_tag="PLL14_03275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOG02974.1"
                     /translation="MKALLDLFRQVTQEEQFDAITIGLASPDKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERM
                     AHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVFDPGMTPLGRGQLL
                     TEDDYLAKVEEYGDDFYAAMGAEGIRELLRSIDLGREVETLRSELETTTSETKSKKFS
                     KRLKILEGFQKSGIKPEWMVLEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNRLEVMGLAT
                     TIKQAKKMVETQEAVVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPTLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDIVL
                     GLYYATRERINAKGEGMLFPDLAEVTRAMQAGELDIHARISVRITEYESDGESGWQQK
                     ITRYNTTAGRALLSEILPKGLPFSLIDKSLKKKEISKLINAAFRRCGLKETVVFADKL
                     MQNGYRLATRAGISICSDDMLVPAQKREIIAAAEEEVKEIENQYTNGLVTNGERYNKV
                     VDIWGRTGDQVAKVMMDQLGHVEVTNRHGEKEKQESFNSIFMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITTNFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVTQDLVVIEEDCGTSNGVTMKALIEGGEVIEALRERILGRVTASDVVNPDTQET
                     VIEQGTLISEDHCDVIDQLGVDEVKARTPLTCETRYGLCAKCYGRDLGRGTPVNVGEA
                     VGVIAAQSIGEPGTQLTMRTFHVGGAASRAAVASHVETRSAGVVRFTATMRYVTSAKG
                     EKIVITRSGEVLITDDNGREREKHKVPYGATLQVADAQAVKAGARLASWDPHTRPIIA
                     EYSGLVKFENVEEGVTVAKQIDEVTGLSTLVVIDPKRGGKAPSKGLRPQVRLFDAAGE
                     EVKMHGSDHAVTITFQVGAIINVLDGQQISVGDVLARLPQESAKTRDITGGLPRVAEL
                     FEARTPKDAGMLAEYTGTMSFGKDTKGKQRLVITDLEGATHEYLILKDKHVLVHDGQV
                     VNKGESIVDGAPDPHDILRLLGVEALAIYITDEVQDVYRLQGVKINDKHIEVIVRQML
                     RRVNVVDPGDTKFIKGEQVERAHLLDENDRMHAEGKLPATYEHVLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGLAYHTTRKKNVAGED
                     IAAGSSLLAEDEPVPSMESAQDAQP"
     gene            complement(4345..>5579)
                     /locus_tag="PLL14_03280"
     CDS             complement(4345..>5579)
                     /locus_tag="PLL14_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG02975.1"
                     /translation="KRAQSIIDDHLRSYKLDLADQLRIVERDAFARAERMIVGKPANG
                     GPKRLAKGTLVAQDYLDGLDPHHWFDIRMADEDIAQQLESLREGLEQTRKGFDVAFEE
                     KRKKLTQGDELPPGVQKMVKVYVAVKRRLQAGDKMAGRHGNKGVVSRIVPVEDMPYMA
                     DGRPVDIVLNPLGVPSRMNVGQILEVHLGLAAKGLGFKIGEMLRRQATAREVRGFLDE
                     IYNSTGKSEELDLLNDAEILEMAGNLEAGVPFATPVFDGAKESEIKSVLALAGMPQSA
                     QMTLFDGRTGEQFERQVTVGYMHVLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFG
                     GQRFGEMEVWALEAYGASYVLQEMLTVKSDDVNGRTKVYENIVKGDHKIDAGMPESFN
                     VLVKEIRSLAIDIDLERF"
BASE COUNT         1045 a         1778 c         1558 g         1198 t
ORIGIN      
        1 ttgccgccaa accttgctac gactgcgctt cgatagtcat gcttcagggc tgggcatcct
       61 gagcgctctc catgctcggt accggttcgt cctcggccag cagcgagctc ccggcagcaa
      121 tatcctcgcc agccacgttc ttcttgcggg tggtgtgata agcaagaccg gtacccgccg
      181 gtatcagacg accgacaatg acattctcct tgaggccgcg caattcgtcg cgtttgccca
      241 tgatcgccgc ttcggtcaga acgcgcgtgg tttcctggaa agaggccgca gagatgaacg
      301 aatcggtaga caaggaggct ttggtaattc ccagcaacac gtgttcgtag gtcgcaggga
      361 gtttgccttc agcatgcatg cgatcgtttt cgtcgagcag atgcgcacgt tcgacctgct
      421 cccccttgat gaatttggtg tcgccagggt ccaccacatt gacgcggcgc agcatctgac
      481 gaacgatcac ttcaatgtgc ttgtcgttga tcttgacacc ctgcaggcgg tacacgtcct
      541 ggacctcatc agtgatgtag atggccaaag cctcgacgcc caacaaccgc aaaatgtcgt
      601 gcgggtccgg cgcgccatcg acgatcgact cacccttgtt caccacctgt ccatcatgca
      661 ccagaacgtg cttgtccttg agaatgaggt attcgtgtgt cgcaccttca aggtcggtaa
      721 tgaccagccg ctgcttaccc ttggtgtctt tgccgaagga catggttccg gtgtattccg
      781 ccagcatgcc ggcatccttt ggcgtacgcg cctcgaaaag ttccgcgacg cgcggcagcc
      841 ccccggtaat gtcgcgggtc tttgcagatt cctgcggcag acgcgccagc acgtcgccga
      901 ccgagatctg ttgaccatcc aggacgttga taatcgcgcc aacctggaag gtgatggtca
      961 ccgcgtgatc gctgccgtgc atcttgactt cttcgccagc ggcgtcaaaa agccgcacct
     1021 gcgggcgcag ccccttgctt ggcgccttgc caccgcgctt cgggtcaatc accaccagcg
     1081 tcgaaagtcc cgtcacttcg tcgatctgct tggcgacggt caccccttcc tcgacgtttt
     1141 cgaacttcac cagacccgag tattcggcga tgatcggacg cgtgtgtgga tcccagctcg
     1201 ccaggcgcgc cccggccttg accgcctgag cgtcggccac ttgcagggtt gcgccgtagg
     1261 gcaccttgtg cttctcccgc tcgcgaccgt tgtcgtcggt aatcagcacc tcgcccgagc
     1321 gcgtgatgac gatcttctct cccttggcac tggtgacata gcgcatcgtt gccgtgaagc
     1381 gcaccacgcc ggcgctgcga gtctcgacat ggctggcaac cgccgcccgt gaagctgccc
     1441 caccgacgtg gaaggtgcgc atggtcaact gcgtgcctgg ctcgccaatc gactgcgcgg
     1501 cgatcacccc gaccgcttcg ccaacattga ccggcgtacc acggccaagg tcgcgaccat
     1561 agcacttggc acaaagtcca tagcgcgtct cgcaggtcag cggcgtgcgt gccttgactt
     1621 cgtcgacccc caactggtcg atcacgtcgc agtggtcctc gctgatcagc gtgccctgct
     1681 cgatgaccgt ctcctgagta tccgggttca ccacatcaga ggcggtcacg cgcccgagga
     1741 tgcgctcgcg caaagcctca atgacctcgc caccttcaat caaggctttc atggtcacgc
     1801 cgttgctggt tccgcaatcc tcctcgatca cgacgaggtc ctgcgtgaca tcgaccagac
     1861 gccgggtcag gtaacccgag ttggcggttt tcagggcggt atcggccagg cccttgcggg
     1921 caccgtgggt agagatgaag tattgaagaa cgttcagccc ttcgcggaag ttggtagtga
     1981 tcggtgtctc gatgatcgaa ccgtcaggct tggccatcag gccacgcatg ccggccaact
     2041 ggcgaatctg cgccgcggaa ccgcgcgcac cggaatcggc catcatgaag atcgagttga
     2101 aggactcctg cttctccttc tcgccgtggc ggttggtgac ttccacgtgc ccgagctggt
     2161 ccatcatcac cttggcgacc tggtcaccgg tgcgccccca gatgtcgacg accttgttgt
     2221 aacgttcacc gttggtcacc agaccgttgg tgtactggtt ctcgatttcc ttgacctctt
     2281 cttcggcagc cgcaatgatc tcgcgcttct gcgccggcac cagcatatcg tcggagcaga
     2341 tcgagatacc ggcgcgggtg gccaggcggt aaccgttctg catcagcttg tcggcgaaga
     2401 cgacggtctc cttcaggccg cagcggcgga aggcggcatt gatcagcttc gagatttcct
     2461 tcttcttgag gctcttgtcg atcaggctga aaggcaggcc cttcggcagg atttcggaga
     2521 gcaaggcacg cccggccgtc gtgttgtagc gcgtaatctt ctgctgccaa ccgctttcgc
     2581 cgtcgctctc gtactcggtg attcgtaccg aaattctggc atggatgtcc agttcgccgg
     2641 cctgcatcgc gcgtgtgacc tccgccaggt cggggaacag cataccctcc cctttggcat
     2701 tgatccgctc gcgagtggcg tagtagaggc cgagaacgat atcctgcgac ggaacgataa
     2761 tcggctcgcc gttcgccggc gacaagacat tgttcgacgc cagcatcagc gtacgcgctt
     2821 ccatttgggc ttcgagcgac aagggcacgt gcaccgccat ctggtcacca tcgaagtcgg
     2881 cattgaaggc ggcgcacacc agcggatgca actggatggc cttgccttcg atcaaggtcg
     2941 gttcaaacgc ctgaataccc agtcggtgca gcgtgggcgc acggttgagc atgatcggat
     3001 gctcgcgaat cacctcctcg aggatgtccc agaccaccgc ctcctgcgtc tcgaccattt
     3061 tcttggcctg tttgatggtc gtggccaggc ccatgacctc gagacggttg aaaatgaaag
     3121 gcttgaacaa ctccagcgcc atcagcttgg gcaagccaca ctgatgcagc ttgagttggg
     3181 gtccaaccac gatcaccgac cgccccgagt agtccacacg cttgccgagc aggttctggc
     3241 ggaaacgacc tcccttgccc ttgatcatgt cagccagcga cttgagcggg cgcttgttgg
     3301 cgccagtcat cgccttgcca cgacgtccgt tgtctagcag agaatccacc gcctcctgaa
     3361 gcatgcgctt ctcgttgcgc acgatgattt ccggcgcttt cagttcgagg aggcgcttca
     3421 ggcggttatt gcggttgatc acgcggcgat agagatcatt gagatcggaa gtcgcaaacc
     3481 gccccccgtc cagaggcacc agcggacgca ggtctggcgg cagaaccggc aggacttcaa
     3541 gcaccatcca ttccggtttg atccccgatt tctgaaagcc ctccagaatc ttgaggcgct
     3601 tggaaaattt cttgcttttg gtctccgaag tggtcgtttc cagttccgag cgcaacgttt
     3661 cgacttcgcg ccccaaatcg atggaccgca acaattcgcg tatgccttcc gcacccatcg
     3721 cggcgtaaaa gtcatcgccg tactcctcga ccttggccag gtagtcatct tcggtcagca
     3781 actggccacg tcccaggggc gtcatacctg ggtcgaagac tacgaaagcc tcgaagtaaa
     3841 gcacccgttc gatgtcgcgc agagtcatgt cgagcaccat gccgaggcgg ctcggcaggc
     3901 tcttcaggaa ccagatgtga gcaaccggac tggccagttc gatgtgcgcc atccgctcgc
     3961 gacgaacctt ggccagcgtc acttcgacgc cgcatttctc acaaatgacg ccacggtgct
     4021 tgagccgctt gtatttgccg cacaggcatt cgtagtcctt gatcggacca aaaatcttgg
     4081 cgcagaacag accgtcgcgc tccggcttga aggtcctgta attgatcgtc tccggcttct
     4141 tcacttcgcc gtaagaccac gagcggatct tgtccggcga ggcaaggccg atggtgatcg
     4201 catcgaactg ctcttcctgc gtcacctgtc taaacaaatc cagcaatgct ttcatctctt
     4261 gctccagtaa ggagtggggt gtcaggggtg cagagccatc aggcaggagg attcaagttc
     4321 ctcctcaacc cctcggcaaa acaatcagaa acgttcgagg tcgatatcga tcgccagcga
     4381 acgaatttcc ttgaccagca cgttgaagga ctccggcatg ccggcgtcga tcttgtgatc
     4441 acccttgaca atgttctcat ataccttggt gcggccgttg acgtcatcag acttgacggt
     4501 cagcatctcc tgcaacacgt aggaagcacc ataggcctcg agtgcccaga cctccatctc
     4561 gccgaaacgc tggccgccga actgcgcctt gccacccaat ggctgctggg tcaccagcga
     4621 gtacggtccg gtcgaacggg cgtgcatctt gtcatcgacc aggtgatgca gcttcagtac
     4681 gtgcatgtat cccaccgtga cctggcgctc gaactgctca ccggtacgac catcgaaaag
     4741 cgtcatctgc gcggattgcg gcatgcccgc gagggccagc acggacttga tttccgactc
     4801 cttcgcccca tcgaaaaccg gcgtcgcaaa gggtactcca gcctcaaggt tgcccgccat
     4861 ttcgaggatc tctgcgtcat taagcaggtc aagttcttcg gacttgccgg tactgttgta
     4921 aatctcgtcg agaaacccgc gtacttccct ggctgtcgcc tgccgacgca acatttcgcc
     4981 gatcttgaag ccgaggcctt tggccgccag ccccagatgg acctcgagaa tctgtcccac
     5041 gttcatccgc gaaggcaccc caagcgggtt gagcacgatg tccacgggtc gcccgtcagc
     5101 catgtacggc atgtcctcga ccggcacgat gcgcgaaaca acccccttgt tgccatgccg
     5161 tccggccatc ttgtcacccg cctgcaggcg acgcttgacg gcgacataca ccttgaccat
     5221 cttctgaaca cccggcggca attcgtcgcc ttgtgtcagc ttcttgcgct tctcttcgaa
     5281 ggccacgtcg aagcccttgc gggtctgttc cagaccctcg cgcagtgact ccagttgctg
     5341 tgcaatgtct tcgtccgcca tccggatatc gaaccaatga tgcgggtcga gaccatcgag
     5401 atagtcctgg gccaccagcg tacccttggc cagccgcttc ggcccgccgt ttgccggctt
     5461 gccgacgatc atccgctcgg cacgggcaaa tgcgtcgcgc tcgacgatgc gcaactggtc
     5521 cgcgaggtca agtttgtagc tgcgcaggtg gtcgtcaatg atcgactgcg ctcgcttgt
//
LOCUS       DAOSIT010000085         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13112_length_5561_cov_10.088449, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000085 DAOSIT010000000
VERSION     DAOSIT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_5561_cov_10.088449"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3043
                     /locus_tag="PLL14_03285"
     CDS             <1..3043
                     /locus_tag="PLL14_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOG02976.1"
                     /translation="KEEKLQRQADDKSKEKIQKAVLPGEKLQKAPRAEEKIQKAVMPE
                     EKLQKKDDDKLQKAPAPADKLQRKGSDATPTIGGGVQSAIRSQSGGGQPLSSDVRSYM
                     EPRFGADFGNVRVHHDAESASLNNQLSARAFTYQNHVFFSRDQYQPGSSEGKQLLAHE
                     LTHTIQQGHAVQRSPQISTTTTPPPVQRLGISDALDYFADKAYLIPGFRMLTLLLGFN
                     PINRRSTDRSAANLLRALIELMPGGAFITQALDNHGVINKAAAWVEQKVALLGDIGGE
                     IAGGLKRFLDSLSWSDILDLGGVWDRAKRIFTEPIARLIAFGGSVVSELLKMVKDAIL
                     KPLAALAQGTRGYDLLKAILGEDPISGEVVPRTADTLIGGFMKLIGQEEVWENIKKGN
                     AVARAWAWFQGALAGLMGFVRAVPRKVIETLSSLTFQDIISVAGAFTKIAGAFVSIAG
                     EFISWGIGQVISLLEILFSVVAPGVMPYIRKAQAAFNTILKDPIGFVGNLVRAGKQGF
                     ELFAANILTHLKTALIKWITGPLGAAGVYIPKSFSLIEIVKLVLSVLGLTWQNIRGKL
                     LKIIPEPILTGLEKTAGILVTLVKDGPAAAWEQIKSELGELKEQLIAKVTEMISVEVV
                     KAAVMKLVSMLNPAGAVVQAIIAIYNTVTFFIQKIQQIAAVVASFVDSIAAIAAGQVG
                     AAAKKVEQTMASTLTVIIAFLAKFAGLGNIPEKIVGIVRKIRQPIDKGLDRIVEWLGA
                     MLKKLGGAVAQAGLPADPNERLRLGMTAAVAVVDRLPGNRIGQVLITPVLAPIKLRYG
                     FQQLEPMVKDKKWHLRGQINPSITVTTTKEDGSEETLRLNVGDVIKAKYVDGLWVATI
                     ISITRSNVSYRFSDPRKGVDTLSASIFKDNFEKGDITPYVADKRGTFMGGNPPKSSLL
                     ARYKSEGTYYRLIKPPPTDQVQYPRRKGGRWYDVADCDLSHEPTDAVTYWNTEGYQYG
                     PRSPEVRAWMSNLDNYIFEPSSINRSRASRHGEGYRLPARP"
     gene            3046..3462
                     /locus_tag="PLL14_03290"
     CDS             3046..3462
                     /locus_tag="PLL14_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG02977.1"
                     /translation="MDDKDLQKTITGIARKAKSLALQGAFESAIAEYTQGLELLAEPK
                     HASEYAVMLFSGIAEAYYLQGLWDDALKSFGEAIRSEGGLGDPYIHLRLGQIRYRQGE
                     MEKAADELMRAYMGSGKIIFEGEDPRYYALIQPHIE"
     gene            3611..4936
                     /locus_tag="PLL14_03295"
     CDS             3611..4936
                     /locus_tag="PLL14_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG02978.1"
                     /translation="MDHNTANLQTALIWLKTLVTGRLGVVLGKNEGFELPPLDFYHQD
                     SWLAAFLERYEPCYEEFAIFLLAIVPHLQPQFFNRIIAEHLPEGGDFPEFGGVRGANH
                     RGILPTGETAQFLLAGDDLEKRLQVQRILSGEHWLASQHILWLESLPHGEPVMSGRLI
                     VDPEVVDLLTVGKVGRPRFSPDFPAEYIETAMDWEDLVLHPHTVRQIGEIENWIKHND
                     TLLHDWGMKRRIKPGYRALFYGPPGTGKTLTASLLGKQTGKDVFRIDLSRVVSKYIGE
                     TEKNLSKLFEKAEHKAWILFFDEADALFGKRTEIRDAHDKYANQEVAYLLQRIEAYNG
                     LVILASNQRANIDEAFVRRFQSIVHFPMPRAEERYQIWRKTFPPQIAIGHDIDWQQIA
                     LRHELSGAGILNVTHFCAVQVLAEQATRLELKQLEAAIRREYVKEGKIV"
     gene            complement(4933..>5561)
                     /locus_tag="PLL14_03300"
     CDS             complement(4933..>5561)
                     /locus_tag="PLL14_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02979.1"
                     /translation="RLREWQAARLARTHADLLASARFGQAAQFFLSDLYGPKDFSSRD
                     EEVERILPLLIKMLPASALRTLALAVELDALTEELDSAMVAELCRAGLIDGIDEAAYA
                     AAYRFVGCRPARERQIVLIRETGEALDRLARKPLLNGMLKLMRGPAHLAGLGDLHHFL
                     ESGFNAFRGMGSATAFLDSIDRKEQQLLSNLFSSVPCPFLVEAVDTAR"
BASE COUNT         1177 a         1706 c         1756 g          922 t
ORIGIN      
        1 caaagaagag aaactgcaac gccaggcgga cgacaaatca aaggagaaaa tccagaaagc
       61 ggtactgccc ggagaaaaac tccagaaagc gccgcgagcc gaggaaaaga tccagaaggc
      121 ggtcatgccc gaggaaaagc tccagaagaa ggacgacgac aagctacaga aggcgccggc
      181 acccgcggac aagctgcagc gcaaggggag cgacgcgacc ccgacgatag gcggcggcgt
      241 gcagtcggcg attcgcagcc agagcggtgg cggccaaccc ctgtcgagcg acgtgcgcag
      301 ctacatggaa ccgcgtttcg gcgccgattt cggcaacgtc cgcgtgcatc acgacgccga
      361 gtccgccagc ctcaacaacc agctcagcgc gcgcgcattc acctaccaga atcacgtctt
      421 tttctcgcgt gaccagtacc agcccggtag cagcgagggc aagcaactgc tggcgcatga
      481 gctgacgcac accatccagc agggccacgc cgtgcagcgc agcccgcaga tcagcacgac
      541 gacgacaccg ccgccggtgc aacgcctggg tatcagcgac gcgctcgatt atttcgccga
      601 caaggcttac ctgatccccg gattccggat gctgaccctg ctgctcggct tcaacccgat
      661 caataggcgc tccaccgacc gcagcgcggc caatctcctg cgcgccctga tcgaactgat
      721 gccgggtggc gccttcatca ctcaggcgct ggacaatcac ggtgtgatca acaaggccgc
      781 cgcctgggtg gaacagaagg tagcgctgct cggtgacatt ggcggcgaaa tcgccggcgg
      841 tctgaaacgc ttcctcgatt ccctgagctg gagcgacatc cttgacctgg gtggcgtctg
      901 ggaccgcgcc aagcgcatct tcaccgagcc gatagcgcgt ctgattgcct ttggcggctc
      961 ggtggtcagc gagctgctca agatggtcaa ggatgccatc ctgaaaccgc tggcggcgct
     1021 ggcgcaggga acacgcggtt acgatctgct caaggccatc ctcggcgaag acccgatcag
     1081 cggcgaagtg gtgccacgca ctgccgacac cctgatcggc gggttcatga agctgatcgg
     1141 ccaggaagag gtttgggaga acatcaagaa aggcaacgct gtggcgcgtg cctgggcgtg
     1201 gttccagggt gcgctggccg gtttgatggg ctttgtgcgc gcggtgccgc gcaaggtcat
     1261 cgaaacgctg agttccctga cattccagga catcatcagc gtcgccggtg cgttcaccaa
     1321 gattgccggg gcgttcgtca gtatcgccgg ggagttcatc agttggggca tcggccaggt
     1381 catcagcctg ctggagatcc tgttctcggt ggtcgcaccg ggcgtgatgc cgtatatcag
     1441 gaaagcccag gcggccttca acaccatcct caaggacccc atcggttttg tcggcaacct
     1501 ggtgcgcgcc ggcaaacagg gcttcgaact gtttgcggcg aacattctca cgcacctgaa
     1561 aacggcgctg atcaagtgga tcaccggccc cctgggggcc gcgggcgtgt atatcccgaa
     1621 atcgttctca ctgatcgaaa tcgtcaagct cgtactttcg gtgctcgggc tgacctggca
     1681 gaacatccgc ggcaaactgc tgaagatcat ccccgagccg atcctgaccg ggctggaaaa
     1741 gaccgccggg atcctggtca cgctggtgaa ggatggtcca gcggcagcct gggagcagat
     1801 caagagcgaa ctgggcgagt tgaaggagca actgattgcc aaggttaccg aaatgatctc
     1861 ggtcgaggtc gtcaaggccg cggtgatgaa gctggtcagc atgctcaacc ccgccggcgc
     1921 ggtggtgcag gcgatcattg ccatctacaa cacggtcact ttcttcatcc agaaaattca
     1981 gcagattgcc gccgtggtgg catccttcgt cgattccatc gccgccatcg ccgccggtca
     2041 ggtgggcgcg gcggcgaaga aggtcgaaca gacgatggcc agcacgctga ccgtgatcat
     2101 cgccttcctc gccaagttcg ccggactcgg caacatcccc gagaagatcg tcggcatcgt
     2161 caggaagatc cgccagccga tcgacaaggg gctggacagg atcgtcgagt ggctgggggc
     2221 gatgctcaag aagctgggcg gcgcggtggc gcaggccggt ttgccggctg atcccaacga
     2281 acgcctgcgc ctggggatga ccgctgccgt ggcggtggtg gaccgcctgc ccggaaatcg
     2341 catcggtcag gtgctgatca cgccggtgct ggcgccgatc aaactgcgtt acgggtttca
     2401 gcagcttgaa ccgatggtca aggacaagaa atggcatctg cgcgggcaga tcaatccctc
     2461 gattacggtg accaccacca aggaagacgg atcggaggaa acgctgcgcc tgaacgtcgg
     2521 cgatgtcatc aaggccaaat atgtcgatgg actgtgggtg gcaacgatca tctcgattac
     2581 ccgcagcaat gtcagctacc gattctctga tccccgcaag ggagtcgata ccctgagcgc
     2641 cagcattttc aaggacaact tcgagaaagg cgacatcacc ccctacgtcg ccgacaagcg
     2701 cgggactttc atgggcggaa atccgcccaa gagcagcctg ctggcgcgat acaagagcga
     2761 agggacgtat taccggctga tcaagccacc gccgaccgat caggtacaat accccagacg
     2821 aaaaggcggc cgctggtatg acgtggcgga ctgcgacctc agtcatgagc cgacggacgc
     2881 ggtgacctac tggaataccg agggatacca atacggaccg cgctcgccgg aagtgcgcgc
     2941 gtggatgagc aatctcgaca attacatctt cgagccatcg agcatcaacc gctcgcgagc
     3001 ctcccggcat ggcgaaggat atcgattacc ggccaggccc tgaacatgga tgacaaagac
     3061 ctccagaaga cgatcaccgg catcgccaga aaagccaagt cgttggcgct gcagggagcc
     3121 ttcgagtcgg ccatcgcaga atatacacag gggctcgaat tgctggcgga gccaaagcac
     3181 gcctcggaat acgcggtgat gctgttctcc gggatcgccg aggcttatta cctgcagggt
     3241 ctctgggatg acgccctgaa gtctttcgga gaagcgattc gcagcgaggg gggcctgggc
     3301 gacccctata ttcacctgcg actgggccag attcgttacc ggcagggcga gatggaaaag
     3361 gctgccgacg aactgatgcg agcctacatg ggttcaggga agatcatctt cgagggtgag
     3421 gaccccaggt actacgccct gatccagccg catatcgaat agtcgcgccg gtttcccgcg
     3481 gcagccctcc tgtcctggcg agtccccggc tcgggctggc gcagaccaga agcctgcacg
     3541 gagccacgca tcaccgccag gcgcccgcac ccttgagcag caccgatacc gagccacgac
     3601 gcgccaggac atggatcaca acaccgccaa tctgcaaacc gccctgatct ggctgaagac
     3661 actggtaacg ggtcgcctgg gtgtcgtcct cgggaagaac gagggtttcg aacttccgcc
     3721 gctcgacttc taccaccagg actcctggct ggccgctttc ctcgaacgct acgagccgtg
     3781 ctatgaagag tttgcgatct tcctgctggc cattgtcccg cacctgcaac cgcagttctt
     3841 caaccggatc atcgccgagc acctccccga aggtggcgat ttcccggagt tcggcggtgt
     3901 gcgcggagcc aaccaccggg gcatcctgcc tacgggagaa accgcccagt tcctgctggc
     3961 cggcgacgat ctggagaagc gtctgcaggt tcagcgcatc ctgagtggcg aacactggtt
     4021 ggcgagccag catatcctgt ggctggaatc gttgccgcac ggcgagccgg tgatgagcgg
     4081 ccggctgatc gtcgatcccg aggtggtcga cctgctgacc gtcggcaaag tcggcagacc
     4141 ccggttcagc ccggactttc cggccgaata catcgaaacg gcgatggact gggaagatct
     4201 ggtgctgcac ccgcacaccg tccgtcagat cggcgaaatc gaaaactgga tcaagcacaa
     4261 cgacaccctg ctgcatgact gggggatgaa gagaaggatc aagcccggct accgggccct
     4321 gttctacggg ccgccgggta ccggcaagac cctgaccgcg tcgctgctcg gcaagcagac
     4381 cggcaaggac gtattccgca tcgatttgtc gcgtgtggtc tcgaaataca tcggcgagac
     4441 cgagaaaaac ctctcgaagc tgttcgagaa ggccgaacac aaggcgtgga tcctgttctt
     4501 cgacgaagcc gacgcgctgt tcggcaagcg caccgaaatc cgcgacgcgc acgacaagta
     4561 cgccaaccag gaagtggcgt acctgttgca gcgcatcgag gcctacaacg ggctggtgat
     4621 cctggcatcc aaccagcgtg ccaacatcga cgaggctttc gttcggcgtt tccagagcat
     4681 cgtccatttc ccgatgccga gagccgagga gcgttaccaa atctggcgga agaccttccc
     4741 gccgcaaatc gccatcggcc acgacatcga ctggcagcag atcgccttgc ggcatgaact
     4801 gagcggcgcc ggcattctca acgtcacgca tttctgcgcg gtgcaggtgc ttgccgagca
     4861 ggccacccgc ctggaactca aacaactgga ggcggcgatc cggcgggagt atgtcaagga
     4921 aggaaaaatc gtctagcggg ccgtgtcgac ggcctcgacc aggaaagggc agggcacgct
     4981 ggagaagagg ttgctcagca actgctgctc cttgcggtcg atcgagtcga ggaaggcggt
     5041 ggccgaaccc atgccgcgaa aagcattgaa gccgctttcg agaaagtggt gcaggtcgcc
     5101 gagcccggcc aggtgcgccg gcccgcgcat cagcttgagc atgccgttga gcaagggctt
     5161 cctggccagt cgatcgagcg cttcgccggt ttcgcggatg agcacgatct ggcgctcgcg
     5221 cgccggccgg cagccgacga atcggtaggc cgcagcgtag gcggcttcgt cgatgccgtc
     5281 gatcagcccg gcgcggcaga gttcggcgac catggccgag tcgagttcct cggtcagcgc
     5341 atcgagttcg acggccagcg ccagggtgcg cagcgccgag gccggcaaca tcttgatcaa
     5401 tagcggcagg atgcgctcga cctcctcgtc gcggctgctg aagtccttcg ggccatagag
     5461 gtcggagaga aagaattgcg cggcctgtcc gaagcgggcg ctggcgagca ggtcggcgtg
     5521 cgtgcgcgcc agacgcgcgg cctgccactc gcgcaggcgc a
//
LOCUS       DAOSIT010000086         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13113_length_5561_cov_9.268434, whole genome shotgun sequence.
ACCESSION   DAOSIT010000086 DAOSIT010000000
VERSION     DAOSIT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13113_length_5561_cov_9.268434"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..841
                     /locus_tag="PLL14_03305"
     CDS             122..841
                     /locus_tag="PLL14_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02980.1"
                     /translation="MHAHGGLRRGHSRQVARALGQCDRGYRRGVAAALLGTAALLGSV
                     LLAAACTPYVEQQGNAPGAVLRPHHSALEACKVSEESYRRVLAEWLQTRDAATPPPDS
                     LALGRAMEYPWISRYLADAAMHSAAWRYGRVRATPGRHNAFVAALLSESNLLQRLDQP
                     FVGSSLQVRGVAVEKVLLGPASEYASVSGASARLKVPFDAQLWLVLGPRPQDSSLAQA
                     INDAQTRGSPGGNKAAEQTHQ"
     gene            complement(758..1927)
                     /locus_tag="PLL14_03310"
     CDS             complement(758..1927)
                     /locus_tag="PLL14_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02981.1"
                     /translation="MKALPLSYIARNLWVRRVTTVLTALGMALVVYVFATVLMMSEGI
                     RATLVATGQPDNVVVLRKGAGAEINSAIARAQAAVVESLPGIATDALGRRLVSKEPVA
                     LINLPKRGSGKPSNVTVRGTSDLGLALRPQVRLAAGRMFRPGTSEIVTGHAIADGFVG
                     AGRGETLRFGGRDWTVVGVLDAGRSGFDSEIWGDSEQMMQAFRRRDFSSMVVRLADAG
                     DFERTRAAVDSDPRLQLEAKPEIRFYAEQSEALATFISILGTALSVIFSIGAIVGAMI
                     TMFAAVAQRVGEIGTLRALGFRRGAVLVAFLAEAMLLALVGGVVGLAAASLMQTVNIS
                     TTNFQTFSELAFHFTLTPGIAVKTLLFALLMGLLGGFIPAWRAARLSIVDCLREA"
     assembly_gap    2002..2101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2102..2853)
                     /locus_tag="PLL14_03315"
     CDS             complement(<2102..2853)
                     /locus_tag="PLL14_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG02982.1"
                     /translation="MLLLLRLLLKNAFRHKLRSALTLIGLVVAICAFGLLRTIVDAWY
                     AGAEASASTRLVTRSAVSLTFPLPLNYGERIRAVQGVSGLSWANWFGGVYVTERNFFP
                     QFAVDPTSYLALYPEYVLPNVQRQAFLRDRQGAIVGRKLADKFGWRVGDQIPLRGTIY
                     PGIWSFTLRGIYNGVDASTDEQQLLFHWKYLSETIRQRPGANPARVDSVGVYIIGIRE
                     ADAAALISQRIDALFRNSLAETLTETEKAFQL"
     gene            complement(2881..3600)
                     /locus_tag="PLL14_03320"
     CDS             complement(2881..3600)
                     /locus_tag="PLL14_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG02983.1"
                     /translation="MNAAAADALPSSLIRIRALSKAYQRGAQHIPVLQGVDLDVQRGE
                     FIALMGPSGSGKSTLLNLIAGIDQPDGGCIEIDGIDIAGLSEGELADWRAANVGFIFQ
                     FYNLLPVLSAAENIELPLLLTDLTRRQRRQHVDAVLAMVRLSDRADHYPNELSGGQQQ
                     RVAIARALVSDPQLIVADEPTGDLDRVTGGEVLSLLADLHRQLGKTIVMVTHDPKAAE
                     RAERLVHLEKGVLVAGTAFGH"
     gene            complement(3597..4889)
                     /locus_tag="PLL14_03325"
     CDS             complement(3597..4889)
                     /locus_tag="PLL14_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG02984.1"
                     /translation="MTEPDIQSAGFPLQPLRIDRSGSAARPVRHRRWLWIAVALLVVA
                     SAVWLLLPRTSEVRTSSVLVSTPSQQYVQLVASGYVVAQRRAAVASKASGRLIELHVR
                     EGSVVKKQQVIARIDASDVEAAIVAAQAGVRQAEAGEGQAAATLQLARVEQANAAADL
                     QRTLGLQSRGFVSPQAVDGARRRANSALATVAGAQAGVKLARAARAQAQAQVQVQRVN
                     EDFTLIRAPFDGVVLVKNANVGDIITPFSSAVGAQGAVVTMADMSTLEVEADVSESNI
                     GKVAIGQPVEIVLDALPDVRFRGKVAGIVPTVDRAKATVMTKIQFEKLDPRILPEMSA
                     KASFLSRMPEDADLKPVLAVNPQSLRERDGRSEVLRLGADNRLEAVPVQRGRNLGDLV
                     EVVDARGEGAGALKSGDKLVLSPDAGLASGTVVKVAAK"
     gene            complement(4965..>5561)
                     /locus_tag="PLL14_03330"
     CDS             complement(4965..>5561)
                     /locus_tag="PLL14_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02985.1"
                     /translation="AAGVDPMAVQLSLWESVRTSQDPADFEDYLKQYPDGKFAAIARR
                     RLAALKTASLKEREPTPQPTRPTPEIERPPNKTSPAPPPISKPLPQQPTAFEVMMQGT
                     ATNMTVGTTAQAQFQLRRTGDGVTAAIRVFPPLFATGVLQGQYREGQCMLQGRMNEGF
                     ILKMIGACSPSGFSGQYQAEAPGQVQQGVFRFQSMASN"
BASE COUNT          906 a         1800 c         1852 g          903 t
ORIGIN      
        1 ccgcagtcga attgaacccg cctatcggcc gcgcagcagc gggttccggc tcgccatcgg
       61 gtatcgctga gtgtttccgg ccagcgcggg ttggggatca cagccgcttc aaggtctagg
      121 catgcacgcg cacggcggtt tgcggagagg acattcgcgg caggtcgcgc gggcactggg
      181 ccagtgcgac cgcgggtaca ggcgcggggt cgcggccgcg ctgcttggta cagccgctct
      241 gctcggcagc gtgctgctgg ctgcggcttg tacgccctac gtcgaacagc aggggaacgc
      301 gcccggcgcg gtgctgcgtc cgcatcactc ggcactggaa gcctgtaaag tcagcgagga
      361 gagctatcgg cgtgtgctcg ccgaatggct gcagacccgc gacgcagcga ctccaccacc
      421 agactcgctg gcgctcggcc gggccatgga gtatccgtgg atctcgcgct atctggccga
      481 tgccgccatg cacagtgccg catggcggta cggccgcgtg cgcgccacgc cagggcggca
      541 caatgccttc gtcgctgccc tcctctcgga gtccaacttg ctgcaacgcc tggaccagcc
      601 ttttgtcggc agctctctgc aggtgcgcgg tgtcgccgtc gagaaggtgc tgctcggacc
      661 ggccagcgag tacgcctcgg tcagtggtgc ctcggcaaga ctcaaggtcc ctttcgatgc
      721 gcagttgtgg ttggtgctcg gaccgcggcc gcaagactca agcctcgcgc aggcaatcaa
      781 cgatgctcag acgcgcggct cgccaggcgg gaataaagcc gccgagcaga cccatcaata
      841 gtgcgaagag cagcgttttg acagcgattc ccggcgtcag cgtgaagtgg aaagcgagtt
      901 ccgagaaggt ctggaagttg gtggtcgaaa tattcaccgt ttgcatcaga ctggccgcgg
      961 ccaggccaac gacgccgccg accagggcca gcagcatcgc ctcggcaagg aaggcgacga
     1021 gtaccgcgcc gcgccggaag ccgagcgcgc gcagcgtgcc gatctcgccg acccgctgcg
     1081 cgaccgcggc gaacatggta atcatcgcgc cgacgatggc gccgatcgag aagatcaccg
     1141 acagtgcggt accaaggatc gagatgaaag tggccagagc ctcgctctgt tcggcgtaga
     1201 agcggatctc gggcttggct tcgagctgca atcgcggatc gctgtcgact gccgcacgcg
     1261 tgcgctcgaa atcaccggcg tcggccaggc gcacgaccat gctcgaaaag tcgcggcggc
     1321 gaaaggcctg catcatctgc tcggaatcgc cccagatctc ggagtcgaaa ccgctgcgcc
     1381 cggcgtcgag gacgccgacg acggtccagt cgcgaccgcc gaaacgcagc gtttctccgc
     1441 ggccggcgcc gacgaaaccg tcggcaattg cgtggccggt gacgatctcg ctggtgcccg
     1501 gacggaacat gcgccctgcg gcaagccgca cctgcggccg caatgccaga ccgaggtcgg
     1561 acgtgccgcg cacggtgacg ttgctcggct tgccgctgcc ccgcttgggc aggttgatca
     1621 gggccaccgg ctccttgctg accaggcgac gaccgagcgc gtcggtggcg atgccgggca
     1681 ggctctcgac gaccgccgcc tgcgcgcgcg caatcgccga attgatctcc gcaccggcgc
     1741 ccttgcgcag tacgaccacg ttgtcgggct ggccggtggc gaccagcgtc gcgcggatgc
     1801 cttcgctcat catcagcacc gtggcgaaga cgtagaccac cagcgccatg cccagcgcgg
     1861 tgagcacggt ggtgacccgg cgcacccaga ggttgcgcgc aatgtagctg agtggcaggg
     1921 ctttcatccg acgtgccgca gaccggcgac aatgtcgatg cgactgatgc gccaggccgg
     1981 ccaggcggcg gcgacggcgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nctgagttgg aaggccttct cggtttcggt gagcgtctcg gccagcgagt tgcggaacag
     2161 cgcgtcgatg cgctgcgaga tcagcgcggc ggcgtcggcc tcacggatgc cgatgatgta
     2221 cacaccgaca ctgtcgacac gcgccgggtt tgcgcccggt cgctggcgga tggtttcgct
     2281 gaggtatttc cagtggaaca gcaactgctg ttcgtcggtg ctggcgtcga cgccgttgta
     2341 gatgccgcgc agcgtgaagc tccagatgcc ggggtagatc gtgccgcgca gcggaatctg
     2401 gtcgccgacc ctccagccga acttgtcggc cagcttgcgc ccgacgatgg cgccctggcg
     2461 gtcgcgcagg aaggcctgcc gctgcacgtt gggcagcaca tattccggat acagcgccag
     2521 atatgacgtc ggatcaacag cgaactgggg gaagaagttg cgctccgtga catagacgcc
     2581 gccgaaccag ttggcccacg acaggccgct gacgccctgc acggcacgaa tccgctcgcc
     2641 gtagttgagc ggcaggggaa acgtcagcga gacggcgctg cgcgtcacca ggcgcgtact
     2701 cgccgaagcc tcggcgccgg cgtaccaggc atcgacgatg gtgcgcagca ggccgaaggc
     2761 gcagatcgcc accaccaggc cgatcagcgt cagcgcgctg cgcagcttgt ggcggaaggc
     2821 gtttttcagc aacaggcgca gcagcagcaa catcgggcgt ggctcatcat cggtgttgac
     2881 tcagtgcccg aaagccgtac cggcaacgag cacgcctttt tcgagatgca ccaggcgctc
     2941 ggcgcgttcc gcggccttgg ggtcatgggt gaccatcacg atggtcttgc cgagttggcg
     3001 gtgcaggtcg gcaagcagtg acaagacctc gccgccggtg acgcggtcga ggtcgccggt
     3061 cggttcgtcg gcgacaatca gttgcgggtc gctgaccagg gcgcgggcga tggctacccg
     3121 ctgctgctgg ccgccggaga gttcgttggg gtaatggtcg gcacggtcgc tcagacgcac
     3181 catcgccagc acggcgtcga catgctggcg acgctggcgg cgcgtcaggt cggtgagcaa
     3241 cagcggcagt tcgatgttct cggcggcgct gagcaccggc aacaggttgt agaactgaaa
     3301 aatgaaaccg acattggccg cgcgccaatc ggcgagttcg ccttcactca ggccggcgat
     3361 atcgatgccg tcgatctcga tgcatccgcc atcgggttga tcgataccgg cgatcaggtt
     3421 gagcagcgtg ctcttgcccg agccgctggg gcccatcagg gcgatgaact cgccgcgctg
     3481 gacgtccagg tcgacgccct gcaacaccgg gatgtgctgc gcgccgcgct ggtaggcttt
     3541 cgacaacgcc cggatacgaa tcagtgacga tggcagagcg tcggcagcgg cggcgttcac
     3601 ttcgccgcca ctttgaccac cgtcccgctg gccagccccg cgtccggcga gagcaccagc
     3661 ttgtcgcccg acttcaatgc gccggcaccc tcgcccctgg cgtcgaccac ttcgacgagg
     3721 tcgccaagat tgcggccgcg ctgcaccggt accgcttcga ggcggttgtc agcgccgagg
     3781 cgcaacactt ccgaacggcc gtcgcgttca cggaggctct gcggattgac tgccaacacc
     3841 ggtttgaggt cggcgtcctc cggcattctg gagaggaagc tcgccttggc gctcatctct
     3901 ggcaggatgc gcgggtcgag cttctcgaac tggatcttgg tcatcaccgt cgccttggcg
     3961 cgatcgacag tcggcacgat accggcgacc ttgccgcgaa aacgcacgtc gggcaacgcg
     4021 tcgagcacga tctcgacggg ctgcccgata gccaccttac cgatattgct ctcggaaacg
     4081 tcagcctcga cctccagcgt gctcatgtcg gccatggtca ccactgcgcc ctgcgcgccg
     4141 acggcgctcg agaacggcgt gatgatgtcg ccgacgttgg cgttcttgac cagcaccacg
     4201 ccatcgaagg gcgcgcgaat cagcgtgaag tcctcgttga cccgctgcac ctgcacctgc
     4261 gcctgcgcct gcgcgcgcgc cgcgcgcgcc agcttgaccc cggcctgcgc accggcgact
     4321 gtggccagcg cgctgttggc gcgcctcctg gcgccatcga ccgcctgcgg cgagacgaag
     4381 ccccggctct gcaggccgag tgtgcgctgc aggtcggcag cggcattggc ctgttctacc
     4441 cgcgccagtt gcagcgtcgc cgccgcctgg ccctcgccgg cttcagcctg gcgaacaccg
     4501 gcctgagcgg cgacaatcgc ggcttcgacg tcgctggcgt cgatgcgcgc gatcacctgt
     4561 tgcttcttca ctaccgagcc ttcgcgcacg tgcagttcga tcagccgacc cgaagccttg
     4621 gaggcgaccg cagcccgtcg ctgcgcgacc acgtagccgg aggccaccag ttgcacgtat
     4681 tgctgtgacg gggtgctgac caggacgctg ctggtacgca cctcggacgt ccgcggcagc
     4741 agcagccaga ccgcgctggc gaccaccagc aaggcgacgg caatccacag ccagcggcgg
     4801 tgacgaaccg gccgcgcagc gctgccgctg cggtcgatgc gcagcggttg cagggggaac
     4861 cctgccgatt ggatgtcggg ttcagtcatg gtatcggagg cgggagagga aacgacgacc
     4921 aggccagacg ttcccagcca tgctgtcgac gctgcgccgc acgctcagtt cgaggccatc
     4981 gactgaaagc ggaagacgcc ctgctggacc tgacccggcg cttcggcctg atactggccg
     5041 gagaagccgc tcggcgagca ggcgccgatc atcttgagaa tgaaaccttc gttcatcctg
     5101 ccctgcagca tgcattgtcc ttcgcggtac tgtccctgca ggacgccggt cgcaaacagt
     5161 ggcgggaaga cgcggattgc cgccgtcacc ccgtcgccgg tacggcgcaa ctggaattgc
     5221 gcctgcgcgg tagtgccgac ggtcatgttg gtggcggtac cctgcatcat cacttcgaag
     5281 gcagtcggtt gctgcggcag cggcttgctg atcggcggtg gcgctggtga agttttgttc
     5341 ggcggccgct cgatctcggg tgtcggacgg gttggctgcg gcgtcggttc ccgctctttc
     5401 agcgatgctg ttttcagggc cgccaggcga cgccgggcga ttgcggcgaa cttgccgtca
     5461 gggtactgct tgagatagtc ctcgaagtct gccggatcct ggctggtgcg tacagattcc
     5521 cagagcgaga gctgcaccgc catcggatcg acgcccgccg c
//
LOCUS       DAOSIT010000087         5560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13116_length_5560_cov_11.033424, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000087 DAOSIT010000000
VERSION     DAOSIT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13116_length_5560_cov_11.033424"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PLL14_03335"
     CDS             complement(<1..1037)
                     /locus_tag="PLL14_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOG02986.1"
                     /translation="MRTLFSPLRVGEIDLANRIVMAPLTRNRAAPGQVPSTLAIDYYR
                     QRATAGLIISEATQISPAGQGYLDTPGIYNAEQVAAWQRITAAVHAEGGRMVAQLWHV
                     GRISHVSLQPDGQAPVSSTARRADTKTFTSNGFVDVSTPRALRTDEIPALIASYRHAA
                     RCALDAGFDGVEVHGANGYLIEQFLRDSINDRSDEYGGSIANRTRLLVEVMQAVAGEI
                     GGGRTGLRLSPVTPANDAAQDSNPQALFEQALAQLAPLQLAFIEVVEGQTAGPRDFAP
                     FDYAALRSRFNGPWMVNNGYQRQMALDAVATGAADLVSFGRPFICNPDLVRRLRENAA
                     WNALDPSTLYGG"
     gene            complement(1158..1490)
                     /locus_tag="PLL14_03340"
     CDS             complement(1158..1490)
                     /locus_tag="PLL14_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG02987.1"
                     /translation="MRIPAEVIPPVANLNKEIEGERKTEPVGGAAGLPDEVSASPHGQ
                     TPPFAERGKRRQAATAPVIIQQQEAPPQVERRQAERRSENRPILLDTRTQRGRRQASG
                     DQRINIKV"
     gene            1831..2991
                     /locus_tag="PLL14_03345"
     CDS             1831..2991
                     /locus_tag="PLL14_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG02988.1"
                     /translation="MSSSNKRFLVLALALAGLLGLGYFAYTSFRGAAGPTLPAVPGGS
                     AAKSGTAPPAQALPVETARVLAAALALDASAVGSLRSNESVVMRPETAGRIASINFQD
                     GAAVGKGSLLLVLDAATQAAELDQARANLGLARSNYQRTADLFEKKFISQQALDNAQA
                     ALKVQEAALALAQARFDKMRLLAPFSGIVGIRNISVGDYVKEGQELINIEDISTLKID
                     FRLPESYLAQLKPGQRLEVASDALPGEVFTAVLDAINPLVEAGGRAISLRAHLPNREG
                     RLRPGMFVRVRLIFEQRNQVLLIPEQAVVPDSKAPYVYRVIDGRAKRTPIRTGLRRDA
                     QVEVVEGLSAGDEVVTAGQIKLRDEAAVRVVGDGSAGTPPPATAAATAGPGK"
     gene            2988..>5560
                     /locus_tag="PLL14_03350"
     CDS             2988..>5560
                     /locus_tag="PLL14_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG02989.1"
                     /translation="MKISDLCIRRPVFATVLSLAIMLVGLVSYTRLPVREYPKIDEPV
                     VTVDTTYRGASAEIVESQISKPLEDSLAGIEGVDVITSISRQENSQISVRFKLERNPD
                     SAASDVRDRVSRVRNKLPTDIDEPVIAKVEADANPIIWIAFSSDQHSALAVTDIANRI
                     VKPRLQTLPGAADVRVFGERKFAMRIWLDRDRLAAFALTPQDVEEALRRQNVEVPAGR
                     IESRSREFSVVARTDLSEPAQFAEIIVKQAADAKGSYPVRIADLGRVEIGAASERSSV
                     RFNGRPAVALGVIKQATANPLELSRALRAELPKVISELPPGMTASVAYDSSVFIDRSI
                     DAVFKTIGEAMLLVLLIIFVFLRNFRATLIPLVTIPVSLIGAFALMLVLGFTINTLTL
                     LALVLAIGLVVDDAIVVLENIYRHIEEGMPRRQAAFQGAQEIGFAVVAMTITLAAVYA
                     PVAFMTGRTGKLFVEFALTLAGAVLVSGFVALTLSPMMCSLLLKHETRHGQAYVRVEN
                     FLNWVTAGYRRLLTAALARRWLVMLAFALVAGANVLLLGALKSELAPIEDRGVILGVF
                     LGPEGATLDYTEKYATQLQNIYAQTADVERYFVVAGNPTVSQGISFVGLADWSQRTRS
                     SAAVVKELFPKFSGIPGVLAFPVLPPSLGQSPRERPVNFVIVTSASYTELEQMTRKIL
                     DEVAKNPGFTNVDTDLKLNKPELSVIVNRDKAMDTGVQIETVGRTLETMLGGRQVTRF
                     KREGEQYDVIVQVADIDRATPSDIRDIFVRARDGSMIPLANLLRVNETVSPRELNHFG
                     QRRAVTLTANLTPGYAMGEALTFLEQAAGRVLPPGYAIDYAGQSREFKLSSSSLALTF
                     IL"
BASE COUNT          876 a         1815 c         1912 g          957 t
ORIGIN      
        1 ccgccataca gggtgctggg gtcgagcgcg ttccaggcgg cgttttcgcg cagccggcgt
       61 acgagatccg ggttgcagat gaacgggcgg ccgaaagaga cgagatcggc cgcgcccgtg
      121 gccacggcgt cgagtgccat ctgccgctga tagccgttgt tgaccatcca cgggccgttg
      181 aaccggctgc gcagcgcggc gtaatcgaag ggtgcgaaat cgcgcgggcc ggcggtctgg
      241 ccctcgacca cttcgatgaa cgcgagttgc agtggcgcga gctgggccag cgcctgttcg
      301 aacagtgcct gcgggttgct gtcctgcgcc gcgtcgttgg ccggggtgac cggagacagg
      361 cgcagcccgg tgcggccgcc gccgatctcg ccggcgacgg cctgcatcac ctcgaccagc
      421 aggcgggtgc ggttggcgat cgagccgccg tattcgtcgc tacggtcgtt gatgctgtcg
      481 cgcaggaact gctcgatcag gtagccgttg gcgccgtgca cctcgacgcc atcgaagccg
      541 gcgtccagcg cgcaacgcgc cgcgtgtcgg tagctggcga tcagggccgg gatctcgtcg
      601 gtacgcaggg cgcgcggcgt cgaaacgtcg acgaagccgt tgctggtgaa ggtcttggtg
      661 tcggcgcgtc gggcggttga cgacaccggc gcctgaccat caggttgcag cgagacatgc
      721 gagatccgcc cgacgtgcca caactgcgcg accatgcgtc cgccctcggc atgcacggcg
      781 gcggtgatcc gctgccatgc ggcgacctgt tcggcgttgt agattcccgg cgtgtcgagg
      841 tagccctggc cggcggggct gatctgcgtc gcctcgctga tgatcagtcc ggcggtcgcg
      901 cgttgccggt agtagtcgat ggcgagggtc gatggcacct ggccgggtgc cgcgcggttg
      961 cgcgtcagcg gggccatgac gatgcggttg gccagatcga tttcgccgac gcgaaggggg
     1021 ctgaaaagtg tgcgcatgca gagtccttga agaagtggaa cgggggtgtc ggtacgctct
     1081 ggcgagcgac gggcgcaggc catcgcgaag gcgacccgga atccgtcaag gataccagcg
     1141 aaaggtcggc gcggtgctca gaccttgatg ttgatgcgct gatccccgga ggcttgccga
     1201 cgaccgcgct gcgtgcgcgt gtcgagcagg atcggccggt tttccgagcg gcgctcggcc
     1261 tgtcgtctct cgacctgcgg cggtgcctcc tgctgctgaa taatgaccgg cgccgttgcc
     1321 gcctgccggc gcttgccccg ttccgcgaac gggggcgtct ggccgtgcgg cgacgccgaa
     1381 acctcgtcgg gcagtccggc ggcgccgccg acgggttctg ttttcctttc gccttcgatc
     1441 tccttgttca ggttggcgac agggggtatg acttcggctg gtattctcat tgacccactc
     1501 ccgttccggt gaccgcacgc caatgcaccg gaacgggcgg tcagacaatc gacaggcaat
     1561 cgaatcgacg aacacgacaa gcgggactct agcaccgcgc ctgccggagt tgaaccgaaa
     1621 aagccccccg ttttgccaac aatgtcgcga atgtcgcgac gaggcgcaga aaaacaagcg
     1681 ccgccgctgg gacggtgtcc ggcaggcgcg cgttgtccgg gctttccgat atcatcgggc
     1741 ttcgctacac ttcgccgcct gccgggccac cgggcacggc agtggtgaac tcccttcatt
     1801 gagccgctgc ggcggaagat tcggatcgac atgtcgagca gcaacaagcg ctttctggtg
     1861 ctggccctgg ccctggccgg ccttctcggg cttggctatt ttgcctacac gagttttcgc
     1921 ggcgcggccg gcccgacgtt gccggcggtg cctggcggca gtgcggcgaa gtcgggcacc
     1981 gcgccgccgg cccaggcgct tcccgtcgag acggcgcgcg tgttggcggc tgcgctcgcg
     2041 ctcgacgcca gcgcggtcgg ttcgctgcgc tcgaacgagt cggtggtgat gcgcccggag
     2101 accgcgggcc gcatcgcgtc gatcaacttc caggacggcg cggcggtcgg caagggttcg
     2161 ctgttgctgg tgctcgacgc cgcgacgcag gccgcggaac tcgatcaggc acgcgccaac
     2221 ctcggcctcg cgcgcagcaa ttaccagcgc acggccgatc tcttcgagaa gaaattcatc
     2281 agccagcagg cgctcgacaa cgcccaggcg gcgttgaagg tccaggaagc ggcgctggcg
     2341 ctggcgcagg ccaggttcga caagatgcgt ctgctggcgc cattctcggg catcgtcggc
     2401 attcgcaaca tcagtgtcgg cgactacgtc aaggaagggc aggagctgat caacatcgag
     2461 gatatcagca cgctgaagat cgattttcgc ctgcccgaat cgtacctcgc gcaactcaag
     2521 cccgggcagc gcctggaggt ggctagcgac gcgctgcccg gcgaggtgtt cacggcggtg
     2581 ctcgacgcga tcaatccgct ggtcgaggcc ggcgggcggg ccatttcgct gcgcgcgcat
     2641 ctgccgaacc gcgaagggcg gctgcgcccc ggcatgttcg tacgtgtacg cctgatcttc
     2701 gagcagcgca accaggtgct gctgatcccg gaacaggcgg tggtgccgga cagcaaggcg
     2761 ccttatgttt accgcgtgat cgatggccgg gcgaaacgca cgccgatccg taccgggctg
     2821 cgccgcgatg cgcaggtcga ggtggtcgag ggtctgagcg ccggcgacga ggtggtcacc
     2881 gccgggcaga tcaagctgcg cgatgaggcg gcggtgcgcg tcgtcggcga tggctccgcc
     2941 ggcacgccgc cgccagccac cgcggccgca acggcggggc cgggcaaatg aagatctccg
     3001 atctgtgcat tcgtcggccg gtgtttgcga cggtgctctc gctggcgatc atgctggtcg
     3061 ggctggtttc ctacacccga ctgccggtgc gcgagtaccc gaagatcgac gaaccggtgg
     3121 tcaccgtcga taccacctat cgcggagcct ccgccgagat cgtcgagtcg cagatcagca
     3181 agccgctgga ggattcgctg gccggcatcg aaggcgtcga cgtcatcacc tcgatctcgc
     3241 gccaggagaa cagccagatc tcggtgcgct tcaagcttga gcgcaacccc gattcggcgg
     3301 cgtcggatgt gcgcgaccgc gtctcgcggg tccgcaacaa gctgccgacg gacatcgacg
     3361 aaccggtgat cgccaaggtc gaggccgacg ccaatccgat catctggatc gcgttttcct
     3421 ccgaccagca ttcggcgctg gcggtcaccg acatcgccaa tcgcatcgtc aagccgcgcc
     3481 tgcagacgct gcccggcgcc gccgacgtgc gcgtcttcgg cgagcgcaag tttgcgatgc
     3541 gcatctggct cgatcgcgac cgtctggcgg ccttcgccct gacgccgcag gatgtcgagg
     3601 aggccctgcg gcggcagaac gtcgaggtgc ctgccggccg catcgagagt cgcagccgcg
     3661 agttctcggt ggtcgcgcgc accgatcttt ccgagccggc gcagtttgcc gagatcatcg
     3721 tcaagcaggc ggccgatgcc aagggcagct acccggtgcg catcgccgac ctcggccgcg
     3781 tcgagatcgg tgccgcctcg gaacgcagca gcgtgcgctt caacggccgg ccggcggtgg
     3841 cgctcggggt gatcaagcag gcgacggcca atccgctcga actctcgcgc gcgctgcgcg
     3901 ccgaactgcc gaaagtgatc agcgaactgc cgccgggaat gaccgccagc gtcgcctacg
     3961 attcgtcggt gttcatcgac cgttcgatcg acgcggtgtt caagaccatc ggcgaggcaa
     4021 tgctgctggt gctgttgatc atctttgtct tcctgcgcaa ttttcgggcg acgctgattc
     4081 cgctggtgac cattccggtg tcgctgatcg gtgccttcgc gctgatgctg gtgctcggct
     4141 tcacgatcaa taccctgacc ctgctggcgc tggtgctggc catcggtctc gtcgtcgatg
     4201 acgccatcgt cgtcctcgaa aacatctacc gccacatcga ggagggcatg ccgcgccggc
     4261 aggcggcgtt ccagggcgcg caggagatcg gcttcgcggt cgtggcgatg accatcaccc
     4321 tggccgcggt ctatgcgccg gtggccttca tgaccgggcg caccggcaag ctgttcgtcg
     4381 aattcgcgct gaccctcgcc ggcgcggtgc tggtctcggg tttcgtcgcg ctgacgctgt
     4441 cgccgatgat gtgctcgctg ctgctgaagc acgagacgcg gcacggccag gcctacgtcc
     4501 gggtggagaa cttcctgaac tgggtgaccg ccggttatcg ccgtctgctg accgccgcgc
     4561 tggcgcggcg ctggctggtg atgctggcat tcgcgctggt cgcgggagcc aacgtgctgc
     4621 tgctcggcgc actgaagtcc gaactggcgc cgatcgaaga ccgcggggtg atcctcgggg
     4681 tgttcctcgg gcccgagggg gcgacgctcg actacaccga aaagtacgcc acgcagctcc
     4741 agaacatcta tgcccagacc gccgatgtcg agcgctactt cgtcgtcgcc ggcaacccga
     4801 ccgtcagcca gggcatctcc tttgtcggcc tggccgactg gagccagcgg acacgcagct
     4861 cggcggcggt ggtcaaggag ctttttccga aattcagcgg cattccgggc gtgctcgcct
     4921 ttccggtgct gccgccgtcg ctcgggcaga gtccgcgcga gcggccggtc aatttcgtga
     4981 tcgtcacctc ggcttcgtac accgaactcg aacagatgac ccgcaagatc ctcgacgagg
     5041 tggcgaagaa ccccggcttc accaacgtcg ataccgacct caagctgaac aagcccgaac
     5101 tgtcggtgat cgtcaatcgc gacaaggcga tggacaccgg cgtgcagatc gaaaccgtcg
     5161 ggcggacgct ggaaaccatg ctcggcggcc gccaggtgac ccgcttcaag cgcgagggcg
     5221 agcaatacga cgtgatcgtc caggtcgcgg atatcgaccg ggcgacgccg tcggacattc
     5281 gcgacatctt cgtccgcgcc agggacggca gcatgatccc actggcgaat ctgctgcggg
     5341 tgaacgagac ggtcagcccg cgcgaactca accacttcgg acagcggcgg gcggtgaccc
     5401 tcaccgccaa tctgacgccg ggctacgcca tgggcgaagc gctgaccttc ctcgaacagg
     5461 ccgccgggcg cgtgctgccg cccggctatg cgatcgacta cgccggccag tcgcgcgagt
     5521 tcaagctctc gtcgtcgtcg ctggcgctga ccttcatcct
//
LOCUS       DAOSIT010000088         5547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13143_length_5547_cov_8.087218, whole genome shotgun sequence.
ACCESSION   DAOSIT010000088 DAOSIT010000000
VERSION     DAOSIT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13143_length_5547_cov_8.087218"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..3906
                     /locus_tag="PLL14_03355"
     CDS             301..3906
                     /locus_tag="PLL14_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG02990.1"
                     /translation="MPPARERRQPGARIAILLAIVAECVAAYVDLNTGTAPLFEVFYI
                     LPLSLAAWFSGERMALVLALAAGIIRSSAHTYGLGAGYSVGLHVYNAGEATILFICVG
                     YFVARIRSHIDRLSTADEHLKQALAERDLALSAARREAEALAHKQTFLLTLFDTIPDL
                     IWLKDLDGVYLACNSKFERFFGHPENEIVGKTDYDFMDRELAEFFRNNDHLAVETDGA
                     RMNEEWLTFAADGYHGLFETIKTPMKTAEGKLIGVLGIARDISRQHEAFEALREREEI
                     YSSIVGQASDGIVLIDPETYDFVEFNDAACQMLGHDRDAFARLKLFDLPADHDPDQLR
                     ALMAATLARGKGHFELRQRHRDGSTRDCWISYRVLRIGGRTLVNAIWHDLTEQKASEA
                     ALQEERQLRATIMEAIPGICYTLDHNGCLRYWNQTFERLMERSPEDLKGINALDFFEG
                     DDRRVIHERIVEAFTTGAKVEVEASLVIDNDRRIPFLFNNRRIEMGGQPVLVGVGIDV
                     SALRAAWDALRRLNSELEQRVRERTRELQAANAQLIDTQFALDSVGIGITWADFATGR
                     FIHANRFSAEFLGYSLDELLQLRVWDIDPNFPPEAFAQITRQIRERGHLQFETEQLTK
                     DNRRLPVEMNIYYHAGNGVTPPKLIAFMSDIARRKDAEAALRQAKEASEAASAAKSEF
                     LANMSHEIRTPLNAILGLNFLLKKEDPSSVQLERLTKMEVAGRHLLSLINDILDLSKI
                     EAGRMDLEHTSFHLSAVLDNVASIVRDAAANKGLTLEVDTDGVPLWLWGDVTRLRQSL
                     LNFASNAVKFTERGKICLRSLLLREQDAALQIRFEVEDTGIGLTPEQQTRMFQAFEQA
                     EGSTARKFGGTGLGLALTRRLVELMGGDVGLHSTPGVGSTFWFSVPLQCGHGPMPILV
                     PTGIQGDAESRLSTRHLGARLLLAEDNPINVEVVLEMLHAVGIDVAVAANGREAVERA
                     RQQTFDLILMDMQMPEIGGIEATRLIRGLPQWADRPIIALTANAFAEDRRACLEAGMN
                     DVLTKPVEPGLLYATILHWLAPTVRGERAPAPVQNQRVFDASDDALVILALRTRDGID
                     VNAGLAMLRGKTDKYLALVRQLVDRQSLQMLEMTQVLDAGDQAAAGRMAHSLKGSAAT
                     LGLAFIAERALRLEQLLTAENDPGVRRDAIQMTIAEVLAALHDIKSVVGPAHHPQ"
     gene            3974..>5547
                     /locus_tag="PLL14_03360"
     CDS             3974..>5547
                     /locus_tag="PLL14_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOG02991.1"
                     /translation="MNTMKFQDLVDIGELQALCESFTALTGAVTAILDLEGNILAATG
                     WQRICTQFHRVNPQTESRCRQSDTVLAGCLREGESYNVYRCQNGLVDVAVPIHVGGEH
                     VANFFTGQFFFEAPDTTWFRCQAKEFGFDETAYLAALAEAPIFTERQVRSMMNFLTRL
                     AQIIGEMGLAQVRLQKANQELQRHREHLEDLVRARTAELSLAKDQAEAANRAKSAFLA
                     NMSHEIRTPLNAISGMAHLVSSGGVSARQAGWLDTLDGASRHLLEIINSILDLSKVEA
                     GRMELEITPIRLDAVVASIVDMIAQQAQAKHLHIGLDLEPLSVPLIGDPTCLRQALLN
                     YATNAVKFTATGSVTLRTRVIEHTPGSVVVRFEVEDTGIGIPAETIDRLFRDFEQADN
                     SSTRQFGGMGLGLAMTRHLAHLMGGTAGVSSTPGKGSTFWFTVRLAVQSASMQSAADE
                     QPDEDLSARLARDFSGYRLLLVEDEPINLEVARFLLEEAGLCVDVAADGIQATDLAAS
                     HDYSLILMDMQMPMMDG"
BASE COUNT         1105 a         1720 c         1679 g         1043 t
ORIGIN      
        1 cccatcgatc aggcttgaga gcttgagtaa tccattcacg ggaatccctc cataaaacaa
       61 cggaaatttc gctcctccca ggcccgatcg cttgcgcctt gcctcatgag cctcttgcaa
      121 aactcgctct gcaaggctga tttttcgctg cagggggcta agaaccgagg gggtgggcgg
      181 ccacttctgg catgatacgg ttttcgagtc caacatcata acgcccgccc aatggatact
      241 acccaacgcc aacggttcat gcaacggtgg ttcaagggag ggaaagtgga acagtcgaag
      301 atgcctccag cgcgagaacg caggcaacct ggtgcacgca tcgccatcct gctggcaatc
      361 gtggcagagt gcgttgctgc ctatgtcgac ctgaacacgg gaacagcccc cttattcgag
      421 gtcttctata tcttgccatt gtccttggcg gcatggttct ccggcgagcg catggctctg
      481 gtactcgccc tcgccgctgg catcattcgt tcgagcgcgc atacctatgg tctcggggcg
      541 ggctattcgg ttgggttaca cgtctacaac gccggcgagg caacaattct gttcatctgc
      601 gtcggttatt tcgttgcccg aatcagatca catatcgacc gcttgtccac cgccgacgag
      661 cacctgaaac aggctcttgc cgaacgtgac ctcgccttgt cggctgccag gcgtgaagcc
      721 gaggcactgg cccataagca aacctttctc ctcactctgt ttgacaccat ccccgacctt
      781 atctggctga aagacctgga cggcgtctat ctggcctgca attccaaatt cgagcgcttc
      841 ttcggccatc ccgagaacga gatcgtcggc aagaccgatt atgacttcat ggacagggag
      901 cttgcggaat tcttccgcaa caatgaccac ctcgcggttg agaccgacgg ggcgcgcatg
      961 aacgaggagt ggttaacttt cgcagcggat ggctatcacg gcctgttcga gaccatcaag
     1021 acgccgatga agaccgccga aggcaagctg atcggggttc tcggcatcgc ccgtgacatc
     1081 agccgtcagc acgaggcatt cgaggcattg cgcgaacgtg aggagatcta ttcttcgatc
     1141 gtcggccagg cgagcgacgg catcgtcctg attgacccgg agacatacga tttcgtcgag
     1201 ttcaacgacg ccgcttgtca gatgctgggc catgaccgcg acgcgttcgc cagactcaag
     1261 ctgttcgacc tgccggccga ccatgacccg gatcagctgc gggcgctgat ggccgcaacc
     1321 ctggcgcgtg gcaaaggcca tttcgagctc cgccagcgtc atcgggacgg ttcgacccgc
     1381 gactgctgga tcagctaccg cgtcctcagg atcggtggtc gcaccctggt caacgccatc
     1441 tggcacgacc tgactgaaca aaaggcgtca gaggctgcgc tgcaggaaga gcgccagttg
     1501 cgcgccacca tcatggaagc gattcccggc atctgttaca cgctcgacca taacggatgt
     1561 ctgcgttatt ggaatcagac cttcgaacgc ctcatggagc gctcgcccga ggacctcaag
     1621 gggattaatg cgctcgactt ctttgaaggc gatgaccggc gagtgatcca cgaacgtatc
     1681 gtcgaggcct tcactaccgg cgcgaaggtc gaggttgaag ccagcctggt catcgacaac
     1741 gaccgacgca tcccgttctt attcaacaac cgacggatcg agatgggtgg gcaacccgtg
     1801 ctggtcggcg tcggtatcga tgtgtcggcg ctcagggctg catgggacgc gctcagacgg
     1861 cttaacagcg aactggagca acgcgtgcga gagcgcaccc gtgagttgca agcggcgaat
     1921 gcccagttga tcgataccca gttcgcgctg gacagcgtcg gaatcggtat cacctgggcc
     1981 gacttcgcga ccggccgctt catccatgcc aaccgattct cggccgagtt cctgggctac
     2041 agtctcgatg aactgctgca actgcgggtg tgggacatcg acccgaactt ccctccggag
     2101 gccttcgccc agatcactcg gcagatccgg gagcgcgggc atctccagtt cgagaccgag
     2161 caactgacca aggacaaccg tcgactcccg gtcgagatga acatctacta tcacgccggt
     2221 aacggggtga ccccccccaa gctcatcgct ttcatgtccg acatcgcgcg ccgcaaggat
     2281 gcggaagcgg ccttgcgcca agccaaggag gcatccgaag cggccagcgc cgccaagagc
     2341 gagttcctgg ccaacatgag tcacgagatc cgtaccccgc tgaacgccat cctcggactc
     2401 aattttctgc tgaagaagga agatccctcg tcggtacaac tcgaacggtt gaccaagatg
     2461 gaggtcgccg gacgccacct gctctcgctg atcaacgaca ttctcgacct gtccaagatc
     2521 gaggccggtc gcatggatct ggagcacacc agcttccacc tctcggcggt gctcgacaac
     2581 gtcgcctcca tcgtccggga tgcagccgcc aacaaggggt tgactctcga ggtcgacacc
     2641 gacggagtgc cgctctggct gtggggtgac gttacccgac tgcggcagtc actcctcaac
     2701 tttgccagca atgcggtcaa gttcaccgaa cggggcaaga tttgtttgcg ctcgctactg
     2761 ctgcgggaac aggatgccgc gctccagatc cgcttcgagg tcgaggatac cggcatcggt
     2821 ctcacccccg aacagcagac gcgtatgttc caggcgttcg agcaggccga aggctccacc
     2881 gcgcgcaagt tcggcggcac cgggctcgga cttgcgctga cgcggcgcct ggtcgaactg
     2941 atgggtggag atgttggtct gcacagtacc ccgggggtgg gcagcacctt ctggttcagc
     3001 gtgccgctgc agtgcggaca tggtccgatg ccgatcctgg ttccgaccgg cattcagggc
     3061 gatgccgagt cgcgcctgag cacccggcat cttggcgcgc gcctcctgct cgccgaagat
     3121 aatcccatca atgtcgaggt tgtcctcgag atgttgcacg ccgtcggcat cgacgtggcg
     3181 gtcgccgcga acggtcgcga ggccgtcgag cgggcccgcc agcagacctt cgacctgatc
     3241 ctgatggaca tgcagatgcc ggaaatcggc gggatcgagg ccacgcgtct gattcgcggc
     3301 ctgccccagt gggctgaccg cccgatcatc gccctgaccg ccaacgcttt cgccgaggac
     3361 cgccgtgcct gcctggaggc gggcatgaac gatgtcctga ccaagccggt cgagcccggc
     3421 ctgctctatg caacaatact gcactggctc gcaccaaccg tgcgtggtga acgcgcgccg
     3481 gcccctgtcc agaatcagcg agtgttcgac gcatccgacg atgcgcttgt aattctggcg
     3541 ctacggactc gagacgggat cgatgtcaac gccggcctgg ccatgctcag gggcaagacc
     3601 gataaatacc ttgccctggt gcgccagctg gtcgaccgcc agagcctgca gatgctggag
     3661 atgacacagg tgctggatgc cggcgatcag gcggcggcag gacggatggc gcacagcctg
     3721 aagggctcgg ccgccacgct tggtctggcg ttcattgccg agcgtgcctt gcgtctggag
     3781 cagttgctga cggcagaaaa tgaccctggc gtccgacgcg acgcgatcca gatgaccatc
     3841 gcggaggtgt tggcagcctt gcacgacatc aagtctgtcg tgggacccgc gcatcacccc
     3901 cagtgacggg ctggacgtag aatatccgca ggaacactca tcagagatgt cttcgcacac
     3961 cgccaggaca cacatgaata caatgaaatt ccaggacttg gtggacattg gcgagttaca
     4021 ggcgctgtgc gagagcttca cggcgctgac cggagcggtg acggcgatcc ttgatctgga
     4081 gggaaacatc ctcgctgcca cggggtggca acggatttgc acccagttcc acagggtgaa
     4141 tccccagacg gagtcgcgct gtcgccagag cgataccgtc cttgccgggt gcctgcgcga
     4201 gggggagtcc tacaacgtct atcgctgtca gaatggtctg gtcgacgtcg cggtgccgat
     4261 ccatgtcggt ggcgagcacg tcgccaactt ttttaccggt cagttcttct tcgaagcgcc
     4321 ggacaccacc tggttccggt gccaggccaa ggaattcggc ttcgacgaga cggcttatct
     4381 tgcggcgctg gccgaagcgc cgatcttcac cgaacggcaa gtgcgctcga tgatgaactt
     4441 cctgacccgg ctcgcccaga taatcggcga gatggggctc gcccaggtcc ggctgcagaa
     4501 ggccaaccag gaattgcaac ggcaccgtga gcatctcgag gatctggtgc gggcccgcac
     4561 agcggagctg tcattggcca aggaccaggc cgaggcggcc aaccgtgcca agagcgcctt
     4621 cctggccaat atgagccacg agatccgcac cccgctcaac gccatctcgg ggatggctca
     4681 tctggtgagc agcggcggag tcagcgcgcg acaggccggc tggctcgaca ccctggatgg
     4741 cgccagccgg catctgctgg agatcatcaa ttcgatcctc gacctatcga aggtcgaagc
     4801 gggccggatg gaactggaaa taacaccgat ccgcctcgac gccgtagtgg ccagtatcgt
     4861 tgacatgatc gcccaacagg cgcaggcgaa acacctgcac atcggtctgg atttggagcc
     4921 cttatccgtc ccgctgatcg gcgatccgac ctgcttacga caggcactgc tcaattacgc
     4981 caccaatgcg gtgaaattca ctgcgactgg ttcggtgaca ctgcgcacac gagtcattga
     5041 acacaccccg gggagcgtcg tcgttcgctt cgaggtcgag gataccggga tcggcatccc
     5101 ggccgagacc atcgaccgcc tgttcaggga tttcgagcaa gctgacaatt ccagcacgcg
     5161 ccagttcggt ggcatgggcc ttggtttggc catgacgcgt cacctggccc atctgatggg
     5221 agggacagcg ggcgtgtcca gcacgccggg caagggtagt accttctggt tcaccgtgcg
     5281 cctcgctgtg caatcggctt caatgcagag cgctgccgac gaacaacccg acgaggatct
     5341 ctccgccagg ctggcgcggg atttcagtgg ttatcgcctg ttgctggtcg aagatgaacc
     5401 gatcaacctg gaagtagcgc gatttctgct ggaggaagcc ggcttgtgcg tcgatgtcgc
     5461 ggccgacggt atccaggcga cagacctggc cgcgagccat gactacagtc tcatcctgat
     5521 ggacatgcaa atgcccatga tggacgg
//
LOCUS       DAOSIT010000089         5539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13165_length_5539_cov_8.689460, whole genome shotgun sequence.
ACCESSION   DAOSIT010000089 DAOSIT010000000
VERSION     DAOSIT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13165_length_5539_cov_8.689460"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..2125)
                     /locus_tag="PLL14_03365"
     CDS             complement(227..2125)
                     /locus_tag="PLL14_03365"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HOG02992.1"
                     /translation="MTTIQEFHQQSIENPDAFWGEEAKSIDWHKPFTQVLDFSRPPFA
                     KWFVGGETNLCYNAIDRHAAKRPNDRALVFISTETDQEVVYTFAELKTEVMRMAAIMQ
                     SLGVGKGDRVLVYMPMIAEAMFAMLACARIGAIHSVVFGGFASGSLATRIDDATPKLV
                     VSSDAGMRGGKAVPYKGLLDEAIALAEHKPANVLMVDRGLDKNFNKVAGRDVDYATLR
                     AQFMDADVPVTWLESSEPSYILYTSGTTGKPKGVQRDTGGYAVALSSSLKYIFCGGEG
                     ETFFATSDIGWVVGHSYIIYGPLIGGMCTIMYEGTPLRPDPGIWWQIVEKYKVSVMFS
                     APTAARVLKKHDNAFMHKYDLSSLKHLFLAGEPLDQPTHEWIMGELGLPVIDNYWQTE
                     TGWPILSAMPGVEKTEIRFGTPSFPVYGYNLKIFREDGTVCDPDEKGIVGILPPLPPG
                     CLSTVWGDDARFVSTYFSLFREPQVYSSFDWGIKDKDGYHYILGRTDDVINVAGHRLG
                     TREIEEAIQGHSAIAEVAVVGVADQLKGQMPMAFAVVKNVDSIATPELVAALEKQVMK
                     QVDDSLGAIARPSRVHFVTVLPKTRSGKLLRRSIQALAEGRDPGDLTTIEDPAALEQL
                     QRALGSKA"
     gene            complement(2171..3313)
                     /locus_tag="PLL14_03370"
     CDS             complement(2171..3313)
                     /locus_tag="PLL14_03370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG02993.1"
                     /translation="MENPKQRPRILIVDASRMARATLISQIREQYNFREEVNGESGWQ
                     ALVIDHSIQLVICALSMPMLDGDGLLARVRSSRLARLRQMPVLMIASDDEEANERARV
                     LGASDFISRGIGATELLARIASLLQLAQAQNELRESPERDTQHPGTGLPTRRHIEVQA
                     AQALSQAVRHDGPLSILIIGFDHFGALREQHGDEVAKELLKRLASMLVQKVRKEDSLG
                     HYSGSDLAVVSPGTPFPACESFANRLREAFAVANIAVHGKRLNLSVSIGVANTPVDRP
                     GSAASLLSLAGERLVAARQAGGNRVVACGDKPLNKLPVPRLGHALELISTGHASAVIP
                     HLIDLGKEILPFLDLLEGELKLGLPLADIRKRMLDREQEIQDTRQA"
     gene            complement(3348..4343)
                     /locus_tag="PLL14_03375"
     CDS             complement(3348..4343)
                     /locus_tag="PLL14_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOG02994.1"
                     /translation="MSPFKAYLIEKKEGGASGSLVTMSESELDPGEVTIRVAYSSVNY
                     KDALAATGAGRIIRRYPCVGGIDLSGIVECSTDPRFRSGDAVLATSFDIGVAHHGGYA
                     EYARVPAQWVVPLPAGLSLHAAMALGTAGFTAALGIVRMEENGLTPSKGPIIVTGATG
                     GVGSLAIDMLAGRGYQVTAFTAKETQSDYLKGLGATAVLLRSAVDPARIRPLDKALWA
                     GAVDNLGGNVLAWIVSTMQQGGTIASIGLALDATLNTTVMPFILRGACLLGIDSGYIG
                     EPYRSGVWQRLAGDLRPRHLQGMVRTIGFDELPGAFDAYIQGRAHGRVVVEIAGA"
     gene            4694..5200
                     /locus_tag="PLL14_03380"
     CDS             4694..5200
                     /locus_tag="PLL14_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOG02995.1"
                     /translation="MLRNHTLAVLLSAVLIACSGVGLSHASEQQSLDEPSLLERYTSS
                     AQDLILKGFELIGINYRFGGTNPDTGLDCSGFVQLVFKEAMGILLPRSAREQSEVGAV
                     IDRHELKAGDLVFFNTMRHAFSHVGIYLGDNRFMHAPRTGAEVRVEDMRQSYWIQRYN
                     GARRLFSR"
     gene            5351..>5539
                     /locus_tag="PLL14_03385"
     CDS             5351..>5539
                     /locus_tag="PLL14_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG02996.1"
                     /translation="MNCSVSPSLLALLLATVTFTGAQAQERVLNLYSARHYQTDEALY
                     SGFTRQTGIAIKRIEGKED"
BASE COUNT         1056 a         1652 c         1674 g         1157 t
ORIGIN      
        1 aaccacttgc gcctcgatcg cctgcgtctc tatgaaacgc ccaattgctg ggctgaagca
       61 gttcgcgaat aacttccggc ggacagcaca gaccacgcaa gttgcgggtg gctggaacgg
      121 caggccgtgc gaaccgcaca gacgccctta ggcggcaaaa aaacgcctgc cgccaatctc
      181 ggcggcaggc gcatcagcgt gcgtcgtgac aggtttctgg ttgggatcag gccttgctgc
      241 cgagcgcgcg ttgcagttgt tcaagcgcag cggggtcctc gatggtcgtc agatcgcccg
      301 ggtcgcggcc ttccgcgagg gcctggatcg agcgccggag cagtttgccg gagcgggtct
      361 tcggcagcac ggtgacgaag tgtacgcggc ttgggcgggc gattgccccg aggctgtcat
      421 cgacctgctt catgacctgc ttctcgagcg ccgctaccag ttccggcgtc gcgatgctat
      481 caacattttt caccactgca aaggccatcg gcatttgccc cttcagttga tcggcaacgc
      541 cgaccacggc gacttcggca atcgccgaat gtccctggat ggcttcctcg atttcgcggg
      601 tgcccagacg gtgtccggcc acattgatca cgtcgtcggt gcggccgagg atgtagtgat
      661 acccgtcctt gtccttgatg ccccagtcga aagaggaata gacctggggt tcgcggaaca
      721 gcgaaaagta ggtcgagaca aagcgtgcgt cgtcgcccca gacggttgac aggcaacccg
      781 gtggcagggg cggtaggatg ccgacgatgc ctttttcatc ggggtcgcaa accgtgccgt
      841 cttcgcggaa gatcttgagg ttgtaaccgt agaccgggaa gctcggcgtt ccgaagcgga
      901 tttccgtctt ctcgacgccc ggcatggccg acaggatcgg ccagccggtt tcggtttgcc
      961 agtagttgtc gatgaccggc aggccgagtt cgcccatgat ccactcgtgc gtcggctggt
     1021 cgagcggttc gccggcgagg aacagatgct tgagactcga caggtcgtac ttgtgcatga
     1081 aagcgttgtc gtgtttcttg agcacgcgcg cggcggtcgg tgccgagaac atgacgctga
     1141 ccttgtattt ttcgacgatt tgccaccaga ttccggggtc cggacgcagc ggcgtgccct
     1201 cgtacatgat cgtgcacatg ccgccgatca gcgggccgta gatgatgtag gagtgtccca
     1261 cgacccaacc gatgtccgaa gtcgcaaaaa acgtttcacc ttcgccaccg caaaagatgt
     1321 acttgagcga ggaggaaagc gcgacggcgt agccgccggt gtcgcgttgc acccctttcg
     1381 gtttgccggt ggtgccggag gtgtacagga tataggacgg ttctgaggat tccagccagg
     1441 ttaccggcac gtcggcgtcc atgaactgcg ctcgcagcgt cgcgtagtcg acgtcgcgac
     1501 cggcgacctt gttgaagttc ttgtcgagac cacggtcgac catcagtacg ttggcaggct
     1561 tgtgttcagc cagggcgatc gcctcgtcga gcaggccctt gtagggtact gccttgccgc
     1621 cgcgcatgcc ggcatccgag gagaccacca gtttcggcgt ggcgtcgtcg atacgcgtgg
     1681 ccaacgatcc ggacgcaaag ccgccgaaaa cgactgagtg gatcgcacca atgcgtgcgc
     1741 aggccagcat ggcgaacatg gcctcggcga tcatcggcat gtacaccaga acccggtcgc
     1801 ccttgccgac gcccaggctc tgcatgatcg cggccatgcg catgacttca gtcttgagtt
     1861 cggcaaacgt atagacgact tcctggtccg tctcggtgga gatgaatacc agtgcgcggt
     1921 cattcgggcg cttggccgcg tgacggtcga tcgcgttgta gcaaagattg gtttcgccgc
     1981 caacgaacca cttggcaaat ggcgggcgag aaaaatcgag gacctgcgta aacggtttgt
     2041 gccagtcaat gctcttggcc tcttcacccc agaaggcgtc cggattttcg atggattgct
     2101 ggtgaaactc ttggattgtc gtcatgagac tccctcttca aaaatgtgaa cccacctgca
     2161 ctagtggaaa ctacgcctgc cgggtgtctt gtatctcctg ctcccggtca agcatccgtt
     2221 ttctaatgtc tgccagcggc agacccaact tcaactcccc ttccaggagg tccagaaagg
     2281 ggagtatctc cttgccaaga tcgatgaggt gcgggattac cgcacttgca tggccggtgc
     2341 tgatcagttc gagagcatgg ccaagcctgg ggaccgggag cttgttcaac ggtttgtcgc
     2401 cacaagccac gacacggttg ccgccggcct gtcgtgcggc gaccagacgc tcgccggcaa
     2461 gactcagcaa ggatgctgcc gaccccggcc ggtcgaccgg ggtattggcg acgccaatac
     2521 tgaccgacag attgagacgc ttcccatgta ccgcaatgtt cgccaccgca aacgcttccc
     2581 gcaagcgatt cgcaaaggac tcgcaggcag gaaagggtgt gcctggcgaa accacagcca
     2641 ggtcgcttcc ggaatagtgg cccaggctgt cttccttgcg tactttttgt accagcatgc
     2701 tggccaggcg tttgagcaac tccttggcca cctcgtcgcc atgttgttca cgcagtgcgc
     2761 cgaagtggtc gaagccgatg atcaggatgc tcagggggcc atcgtgccgg acggcctgcg
     2821 acagtgcctg tgccgcctgc acctcgatgt gtcggcgagt cggtaggccg gttcccggat
     2881 gttgagtgtc tcgctccggg ctctcgcgca gttcgttctg cgcttgtgcc aactgcagca
     2941 gcgaggcaat acgggcgagc agttcggtgg ctccgatgcc cctgctgatg aagtcggaag
     3001 cacccagtac ccgggcgcgc tcattggctt cttcgtcgtc gctggcgatc atcagtaccg
     3061 gcatctgacg aaggcgggcc aaccgggaag agcggactct tgccagcaga ccgtccccat
     3121 ccagcatcgg catcgacagc gcgcagatca ccaattggat ggaatggtcg atcaccagtg
     3181 cctgccaacc cgactcacca ttgacctcct cgcgaaagtt gtactgctca cgaatctgac
     3241 tgatcagcgt tgcccgcgcc atccgtgaag catcgacaat cagtatgcga ggacgctgct
     3301 tgggattctc catcgaatct cgcgattcct gtcaagccgg gtgcgtctta cgcgccggcg
     3361 atttcgacga ccacgcggcc gtgtgcgcga ccctgaatgt aggcgtcgaa ggccccggga
     3421 agttcgtcga aaccgatggt gcgcaccatc ccctgcaagt gtctcggtcg cagatcgcct
     3481 gccaggcgct gccaaacacc gctgcggtac ggttcgccga tgtaacccga atcaatcccg
     3541 agcaagcagg ctccgcgcag gatgaagggc atcaccgtgg tgttgagtgt cgcgtcgagt
     3601 gccaggccaa tgctggcaat cgtgccgccc tgctgcatgg tgctgacgat ccaggccagc
     3661 acattgccac cgaggttgtc cacggcgccc gcccacaggg ccttgtctag cggccgtatg
     3721 cgggctgggt cgacggctga gcggagcaag accgctgtcg caccgagtcc cttcaggtaa
     3781 tcggactggg tttccttggc agtgaaggcg gtgacctgat agccccggcc ggccagcatg
     3841 tcgatcgcca gactgccgac tccgccggtc gcgcctgtga cgatgatcgg tcccttgctc
     3901 ggcgtgaggc cattctcttc catgcgcacg atgccgagcg ccgccgtaaa tccggctgta
     3961 cccagggcca tggcggcatg caacgaaagc cctgcgggca gcgggactac ccattgagcg
     4021 gggactctgg cgtactcggc gtagcctccg tggtgggcga cgccgatgtc gaagctcgtg
     4081 gccagcacgg catcaccgct tcggaagcga gggtcggtac tgcattcgac aattcccgac
     4141 aggtcgatgc caccgacaca ggggtagcgg cggatgattc gcccagcgcc ggttgccgcc
     4201 agggcatcct tgtagttcac gctggagtag gcaacccgga tggtcacttc gccgggatcg
     4261 agttccgact cgctcatggt gaccaggcta ccgcttgccc caccttcctt tttctcgatc
     4321 agatacgctt tgaaaggact catgcacact ccatgcgatg gataaggttg gcaaattgac
     4381 gatgggaaga agccatcgcg tcatgaacgg tcgtcgatta taaacacaaa ccccggcact
     4441 tttgagcttc ggcgaaagcg gcccgcgtgc cgccgaatcg cctttgtgga ggcgaaagcc
     4501 gcgacggcgt cagactatac cgcgttccat tgtccaatcc catgtgcagt gcaacaaatc
     4561 cgtagccagc aataaatcgt caatatcgtc attaagttat ggtgttgcga tcatctgttg
     4621 aggtgttttg tgctgcacct gctgcacgat agggggttta cagcgtgaca tttttcacat
     4681 aaattccgcc cctatgctca gaaatcacac tcttgccgta cttttaagcg ccgtcctgat
     4741 tgcttgttcc ggggtcggct tgtcccatgc cagcgagcag cagtcgctcg acgagccgtc
     4801 gttgctggag cgctacacca gcagcgcaca ggatctgatc ctcaagggct tcgaactgat
     4861 cggcatcaac tatcgctttg gtggtaccaa tccggatacc ggtcttgact gcagcggctt
     4921 cgtccaactc gtcttcaagg aagccatggg gatattgctg ccgcgcagtg ccagggagca
     4981 gagcgaggtc ggggcggtga tcgaccgtca cgaactgaag gcaggcgacc tcgttttctt
     5041 caataccatg cgccatgctt tttcgcacgt aggtatctat ctcggcgaca accgcttcat
     5101 gcacgcgccg cgtaccggcg ccgaagtccg cgtcgaagac atgcgccaaa gctactggat
     5161 tcagcgctac aacggtgctc gccggttgtt ctcacgttga cctgcagttt gcaatgagcc
     5221 tggagacgac gcacagcgtc gtcttttttc tatttatatc gagaacgatt cgcatttact
     5281 tgttcgttgg tgccggctat catgtgggct tgcgacagca attttcattt tgataaccgg
     5341 aggcatggcc atgaattgtt ccgtttcccc ttcgctcttg gctctgttgc ttgctaccgt
     5401 cacgttcacc ggtgcgcagg cgcaggaaag ggtgctgaat ctctactcgg ctcgccatta
     5461 ccagacggat gaggccttgt acagtggctt cacccggcag accgggattg ccatcaagcg
     5521 tatcgagggc aaggaagac
//
LOCUS       DAOSIT010000090         5490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13315_length_5490_cov_8.429439, whole genome shotgun sequence.
ACCESSION   DAOSIT010000090 DAOSIT010000000
VERSION     DAOSIT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13315_length_5490_cov_8.429439"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="PLL14_03390"
     CDS             <1..2004
                     /locus_tag="PLL14_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOG02997.1"
                     /translation="KHIFANLGDGTYFHSGLLAIRQSVAAGVAITYKILYNDAVAMTG
                     GQPVDGSLSVAQMTRQLEAEGIGKIVVVTDEPARYRQIRDLAPHTPVHHRDQLDAVQR
                     ELREHPGVSILIYDQTCATEKRRRRKRGKLADPARRVFINTAVCEGCGDCGVQSNCLS
                     VIPAATEFGRKRAIDQSSCNKDFSCVKGFCPSFVTVVGGRLRRGRAVATDETLFATLP
                     EPLLPDTGRPFNVLITGVGGTGVITLGALLGMAAHLDGKGVSVLDMTGLAQKYGAVFS
                     HLRIADRPEDIHAARIATGEAHAVIGGDLVVTAGAEAVSKVLAGKTRAVVNVAATPTA
                     EFTHHPDWQFPQQRMQTQIDEACGEGNTLFLDTTEMSRRLMGDSLYTNLLLLGVAWQQ
                     GLVPLSLAAIDKAIELNAAAVEQNRSAFLWGRRAAHNPEAIRRLTRSAPDAAEKEDPT
                     DLDTIISRRVEALTAYQDARYAQRYRRLVERVRVAESALRTTLLSEAVADSYFRVLAV
                     KDEYEVARLYAEPAFRQQLSEAFEGDYHLRFHLAPPLLARPDPATGRIRKRSYGPWMM
                     TAFHWLSRLKVLRGTVFDPFRNTQERQMERQLLADYEDDVELLLAQLKPEALATAVEL
                     ARVPEQVRGFGHVKVASVSQAHSRRTLLRAQLLAATGAPASAP"
     gene            2049..2855
                     /locus_tag="PLL14_03395"
     CDS             2049..2855
                     /locus_tag="PLL14_03395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG02998.1"
                     /translation="MPVPSPEPTDAAPAVPGPQIRQLILQFAASLAVLSLAWPYYGIR
                     NEALPWPQTALAIGAAAGLLASLSRQPWWWRVMHALFAPLAWSVSTLGIDPGWFLLLF
                     IMLLLVYRGAAGGQVPLYFSNRPTAAALARITAELPDLRFLDLGAGIGSVLLPLARER
                     PEGHFTGVENAPATWLLGRLRTAGLANCDWRWGDLWRSDFAAYNVVYAFLSPAPMAAL
                     WNKVEQEMPPGSLLISNSFAVPTIPPTYVIDVDDARQTRLYCYRSGNPRH"
     assembly_gap    3060..3159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3160..4302)
                     /locus_tag="PLL14_03400"
     CDS             complement(<3160..4302)
                     /locus_tag="PLL14_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG02999.1"
                     /translation="MSGGPLRGVRVLDLTRLLPGPVATLHLADMGADVIKIEDHGVGD
                     YARTLGDGPDGVSVFYRSVNRNKRGLRLDLKRAQGREVFLSLARAADIVVESFRPGVS
                     RKLGIDYETLRAINPGIVYCAITGYGQTGPLARAAGHDLNYIGLAGVLDQIGVDGGQP
                     AIPNLQIGDLLGGAMTAVMGILAALFDAQRSGQGRLVDVAMSEAVLAHNLFPFFALQS
                     AGGLPQRGADLLSGGDAGYGVYATADGRHMAVAPLEAKFWDVFCEALGRPEWKTRHAA
                     RGAAAVALRRELEDLFAGRTQAEWRDFFADVDCCVTPVLAVGEALEHPQFVARAMAVH
                     ADGVMQYAPPLKLSGWVFAVDRVAPAPGEHSEELLREVGFTEDEIVT"
     gene            complement(4299..4802)
                     /locus_tag="PLL14_03405"
     CDS             complement(4299..4802)
                     /locus_tag="PLL14_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03000.1"
                     /translation="MVATRAFQAYVAMLLSASALAVETAAVVDAVPPPGPRIGGCAIF
                     REGGAGWLLKAPTYWLKGSIVGISRERRMANLCPQIGKPASAYTQADRLRVAAATPCV
                     LKAAEIGEVSVIRVQLAVEAWETPWSSQHGAGGWLFRGQFLDQTLSKGEVVDIDANWL
                     EPCEAGS"
     gene            complement(4815..5186)
                     /locus_tag="PLL14_03410"
     CDS             complement(4815..5186)
                     /locus_tag="PLL14_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03001.1"
                     /translation="MAKTWLAAAYLLASSVAHASLIITSAPGKLMFAYLGGEAGPSLQ
                     EFGIGTPAEAASLADRNMVFAIDYRLPPVVVPIVNKGYFWAGSELDFYIAAVHSCPLD
                     LLRDACTWTGLLRGCRSTLFL"
     gene            complement(5288..5378)
                     /locus_tag="PLL14_03415"
     tRNA            complement(5288..5378)
                     /locus_tag="PLL14_03415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5342..5344),aa:Ser,seq:gga)
BASE COUNT         1015 a         1843 c         1604 g          928 t
ORIGIN      
        1 aaacacatct tcgccaacct gggcgacggt acctatttcc attccgggct gctggcaatt
       61 cgccagtcgg tcgccgccgg ggtggcgatc acctacaaga ttctctacaa cgatgcggtc
      121 gcgatgactg gcgggcagcc ggttgacggc agcctgagcg tcgcccagat gacgcgtcag
      181 ctcgaggccg aaggcatcgg caagattgtc gtggtcaccg acgaacccgc ccggtaccgg
      241 cagatacgcg atctggcacc gcataccccc gtccatcacc gcgaccagct cgatgccgtg
      301 cagcgcgaac tgcgcgagca tcccggcgtc tccatcctga tctatgacca gacctgcgcc
      361 accgagaagc gacgtcggcg caagcgcggc aagctcgccg atcccgcgcg acgggtgttc
      421 atcaacacgg cggtctgcga aggctgtgga gactgtggcg tgcagtccaa ttgcctgtcg
      481 gtgattcccg ccgcaacgga gtttggccgc aagcgagcga ttgaccagtc ctcatgcaat
      541 aaggattttt cctgtgtcaa gggtttctgt cccagtttcg tcaccgtcgt aggcggacgc
      601 ctgcgccgtg gccgcgccgt cgccaccgac gagactctct ttgcgaccct gcccgagccg
      661 ctgctgcccg acaccgggcg cccgttcaac gtcctgatca ccggcgtcgg cggcaccggc
      721 gtgatcaccc tcggcgcgct gctcggcatg gccgcccatc tggacggcaa aggcgtatcg
      781 gtgctcgaca tgaccggcct ggcacagaaa tacggcgccg tcttttctca cctgcgcatc
      841 gccgaccgcc cggaagacat tcacgccgca cgtatcgcga ccggcgaagc acatgcggtc
      901 atcggcggcg acctggtggt tacggccggc gccgaggcgg tgtcaaaggt cctcgccggc
      961 aagacgcgag cggtggtcaa cgtcgctgcg acgccgaccg cagaatttac gcaccatccg
     1021 gactggcagt ttccgcaaca acgcatgcaa acccagatcg acgaggcctg tggcgaaggc
     1081 aatacgctct tcctggacac caccgaaatg tcccgtcgcc tgatgggtga ctccctttac
     1141 accaacctgt tgctgctcgg cgtggcctgg caacagggac ttgtccccct gtcgctggcg
     1201 gccatcgaca aagccatcga actcaacgcg gccgcggtgg aacagaaccg tagcgctttc
     1261 ctctggggac ggcgcgcggc gcacaacccg gaggccatac gccgcctcac cagatccgcg
     1321 cctgacgctg cagaaaaaga agacccgact gacctcgaca cgatcatttc ccgccgcgtc
     1381 gaggccctga ccgcctacca ggatgcgcgt tatgcccaac gctaccgccg cctggtcgaa
     1441 cgtgtacgcg tcgccgaatc ggcattgcgc accacgctcc tgagcgaggc cgtcgccgac
     1501 agttatttcc gggtgcttgc ggtcaaggat gaatacgagg tcgccaggct ttacgccgaa
     1561 cccgcattcc ggcagcaatt atccgaagca ttcgagggcg actaccacct gcgcttccat
     1621 ctggccccgc cactgctcgc cagacctgac ccggccaccg gccgcatcag gaagcgtagc
     1681 tacggtccat ggatgatgac cgccttccac tggctgagcc ggctcaaggt cttgcgcggc
     1741 accgtcttcg atcctttccg gaacacccag gaaaggcaga tggaaaggca attgcttgcc
     1801 gactacgagg acgacgtcga actcttgctc gcacagctca agccggaagc cctggccacc
     1861 gcagtcgaac tggcgagagt gccggagcag gttcgcggtt ttggtcacgt caaggtagcg
     1921 tccgtgtcgc aggctcactc gcgacggacg ctgctgcggg cgcaactgct ggcagcgaca
     1981 ggcgccccgg cgagcgcacc ctgagcgcag gccctacaat tcgtcgttca gcctccagga
     2041 gcaagccttt gcctgtcccc tcccccgagc ccacggatgc cgcgccagcg gtgcccggcc
     2101 cgcaaattcg ccaactgatc ctgcagttcg cggcatcgct cgccgtgcta tcactggcgt
     2161 ggccctacta cggcatccgc aacgaggcac tgccctggcc gcagacggcc ctcgccatcg
     2221 gcgccgcggc ggggctgctt gccagcctga gccggcaacc ctggtggtgg cgggtgatgc
     2281 atgccttgtt cgcgccgctg gcctggagcg tatcgacgct gggcatcgac cccggctggt
     2341 tcctgctgtt gttcatcatg ctgctgctgg tgtatcgcgg ggcggcgggc ggacaggttc
     2401 cgctctattt ttccaaccgt ccgaccgccg cagccctcgc cagaattacc gccgaactgc
     2461 ctgatctgcg tttcctcgac cttggcgcgg ggatcggcag cgtgctgttg ccgctggcca
     2521 gggagcgtcc tgagggccat ttcacgggtg tcgaaaatgc gcccgcgacc tggctgcttg
     2581 gccgcctgcg caccgctggc ctggccaatt gcgactggcg ctggggcgat ctgtggcgaa
     2641 gcgatttcgc cgcctacaat gtcgtttacg ccttcttgtc accggcgcca atggccgcct
     2701 tgtggaacaa ggtcgaacag gagatgccgc ctggaagcct gttgatcagc aacagttttg
     2761 cggtgcccac catccccccc acgtatgtca tcgatgtcga cgacgcgcgg caaacccgtc
     2821 tctactgcta ccggtccggc aaccctcgcc actgaatcaa gcggcccgcc gcacgacacg
     2881 cattgatcca tcccgacaag actctcctcc cgaaccgacg gtcgagccac ttcgccgaaa
     2941 accccggaat tttgctaaca tgaaccgttc caccaccaca ggagcactct gatgtccaat
     3001 cacgattcgt taacggatcc cctgagcttt gccaagaaaa tgtggggggg catgggattn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtgacgattt catcctcggt
     3181 gaacccgacc tcacgcagca gttcctcgct gtgctcgccg ggcgctggtg cgacccggtc
     3241 gacggcaaag acccagccgg agagcttcag cggtggcgcg tattgcatca ccccgtcagc
     3301 atggaccgcc atggcgcgtg cgacgaactg cggatgttcg agcgcctcac ccaccgcgag
     3361 cacgggcgtc acgcagcagt cgacgtcagc aaagaaatcc cgccactcgg cctgcgtgcg
     3421 accggcaaaa agatcctcga gttcacgacg taaagccact gcggccgcgc cgcgcgccgc
     3481 atgccgcgtc ttccactcag gccgtcccag ggcctcgcaa aagacgtccc agaatttcgc
     3541 ctcgagtggt gcgaccgcca tatggcgccc atcggcggtg gcatagaccc cgtagcccgc
     3601 atcaccaccg ctcagcaggt cggcaccgcg ctgcggcaac ccgcctgcgc tctgcagagc
     3661 gaaaaaggga aagagattgt gcgccaatac ggcctcgctc atcgccacgt cgaccaaccg
     3721 cccctggcca ctgcgctgcg catcgaacaa ggccgcgagg atgcccatca cggccgtcat
     3781 cgcgccgccc agcaggtcac cgatctgcag attcggaatc gccggctgac cgccgtccac
     3841 gccgatctga tcgagaacac cggccagtcc gatatagttg agatcgtgtc cggcagccct
     3901 cgccaagggt ccggtctggc cgtagccggt gatcgcgcaa tacacgatcc cgggattgat
     3961 cgcccgcagg gtttcatagt caatgcccag tttgcggctg acccccggcc ggaagctttc
     4021 gaccacgatg tccgcggccc gcgcgaggct cagaaacacc tcgcgtccct gcgcgcgctt
     4081 gagatcgagt cgcaggcctc gcttgttgcg attgacactg cgataaaaga cgctaacgcc
     4141 gtccggaccg tcgcccaggg tacgcgcata gtcgccgacg ccatgatcct cgatcttgat
     4201 cacatcggcc cccatatcgg caaggtgcag ggtagcgacc ggacctggca acaggcgtgt
     4261 caggtcgaga acgcggaccc cgcgcaatgg accgccgctc atgagcctgc ctcacagggt
     4321 tccagccagt tggcgtcaat gtccacgacc tcccctttgc ttagcgtctg atcgagaaac
     4381 tgtcctcgaa aaagccagcc gccggccccg tgctgcgacg accacggcgt ttcccaggct
     4441 tcgacggcaa gctggacgcg gatcacggac acctcgccga tctcggccgc cttgagcacg
     4501 caaggtgtcg ctgccgccac gcgaagacgg tcggcctgag tataagcgga cgccggcttg
     4561 ccgatctgcg ggcagagatt cgccatccgc cgctcgcgcg agatgccgac gatcgagccc
     4621 ttcagccagt aagtcggcgc cttcaacagc cagcctgcgc caccctcgcg gaagatcgcg
     4681 cagccgccga ttcgcgggcc gggcggcggc acagcgtcga ccaccgccgc ggtctcgacg
     4741 gccagcgctg aagcgctcag caacattgcc acataggcct gaaaagcgcg cgtcgcgacc
     4801 atctcggaaa accgtcaaag gaatagcgtt gatctgcagc cgcgcaggag tccggtccag
     4861 gtgcaggcgt ctcgcagcag atccagcggg caggaatgaa ccgccgcgat gtagaagtcg
     4921 agttcgctcc cggcccagaa gtaccccttg ttgacgatcg gcacaaccac cggaggcagc
     4981 cggtaatcga tcgcgaaaac catgtttctg tcagcgagcg aggctgcttc ggcaggggtg
     5041 ccaataccga attcctgtag cgatggtccc gcctcgcccc cgaggtacgc gaacatcagt
     5101 ttgcctggcg ccgaagtgat gatcaggctt gcgtgcgcaa cgctgctggc aaggaggtat
     5161 gccgcagcaa gccatgtctt ggccatcggt tactcgatct cttatatcgg cgcgtgggtt
     5221 gaaacgggga taggaacaaa gtcgaaaccc gcaaagcctt gtgcagtacg ggtttcaggg
     5281 gttttgctgg cggagagagc gggattcgaa cccgcggtgg gatgttatcc cacacacgct
     5341 ttccaggcgt gcgacttaaa ccactcatcc atctctccgg aagaggacgg attctagcag
     5401 aaggtaaagc caataccaaa cgtgacgcac tgccatcagc cctgacgcat gaccctgccc
     5461 aatcgggttc gtttgcctgt agccccgcat
//
LOCUS       DAOSIT010000091         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13557_length_5410_cov_9.273203, whole genome shotgun sequence.
ACCESSION   DAOSIT010000091 DAOSIT010000000
VERSION     DAOSIT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13557_length_5410_cov_9.273203"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLL14_03420"
     CDS             <1..213
                     /locus_tag="PLL14_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292382.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HOG03002.1"
                     /translation="FMLNALRLIQGFDAQLFESRTSLPLFSIEHILRQAQADGLIERA
                     GQRIAPSDRGRRFLNRLLQRFLADRD"
     gene            complement(248..1405)
                     /locus_tag="PLL14_03425"
     CDS             complement(248..1405)
                     /locus_tag="PLL14_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOG03003.1"
                     /translation="MSLLIGRFGGVMLLVCCLLVGPASADAPAPDPITFGYAVERGDL
                     RTVKRWLDDGLNPEYQAAQIGTGLMVAAWYGNIEMMALFVERGADIRRSNRNGEQPLQ
                     LAAWGGHLDAVKWLLERGAPLEREGKQWGALHYAVFNGHEQVVNDLMARGANVNARSP
                     NGSTPLMLAAREGRDGLARTLLEAGANPKAQSDWGDTALTMAMRYDHLRLAKMISSPE
                     EFAIAVKVPKENFGDAIRSAAVPSAIDAILKEIRAAEAEGRPSEDLHKKLLAAVNELR
                     RGSSGPVVQRPMPRPYQPRSMVITARRSQPGAERAQLLVGGSPAGPDVAPLPGSAAVK
                     TPSRNAAAQTRSLATAQAADLLRQIRLAEAQGQPADGLRQQLYQVVEAMKP"
     gene            1491..2018
                     /locus_tag="PLL14_03430"
     CDS             1491..2018
                     /locus_tag="PLL14_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG03004.1"
                     /translation="MPLRLILALALSLALHAGLLLPDVFGQHTPPQRSVLAASLQLSH
                     GPQEPDRDETLLKNTLLTEKAPVKAPPAPPPAAHAGPAPGKAVTKRQLDAVQRKLAQH
                     QYYPPEAIARGIEGDGSLLLTLAEDGSISEVSIAVSSGHPILDKAAVRAAYAMARVNW
                     AHSRELILPFVFRLE"
     gene            complement(1977..2720)
                     /gene="trmB"
                     /locus_tag="PLL14_03435"
     CDS             complement(1977..2720)
                     /gene="trmB"
                     /locus_tag="PLL14_03435"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG03005.1"
                     /translation="MAGQETAYTPGRAAHIRSFVRRQGRVSNAQQRYHEDMMAKIGVA
                     YVAQCVDFETLFGRAAPRILEIGFGMGETSAAIAALHPERDYLGIEVHTPGVGSLCKL
                     VAERALHNLRIVQHDAVEVVRDMISPASLAGVHVFFPDPWPKKRHHKRRLLQPPFVAL
                     LASRLQPDGYIHCATDWEDYAQQMLAVLAAEPRLQNTAPDYAPRPAYRPLTKFEQRGL
                     RLGHGVWDLVFRRRQDSLQPEDERQDQFA"
     gene            complement(2723..3517)
                     /locus_tag="PLL14_03440"
     CDS             complement(2723..3517)
                     /locus_tag="PLL14_03440"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOG03006.1"
                     /translation="MPSSPHHLTVAGRTYRSRLLVGTGKYPDFTATRAAIDASGAEIV
                     TVAIRRTNIGQDAGEPNLLEVLPPSQFTILPNTAGCYTAADAVRTLRLARELLDGHAL
                     CKLEVLGDPNNLFPNMPETLKAAETLVREGFEVMVYCADDPIQARMLEEIGCVAVMPL
                     ASLIGSGMGIVNPWNLRLIIDNAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAH
                     ARDPVLMASAMKKAVEAGREAFLAGRMPRKFYSADPSSPTTGLIGS"
     gene            complement(3550..3750)
                     /gene="thiS"
                     /locus_tag="PLL14_03445"
     CDS             complement(3550..3750)
                     /gene="thiS"
                     /locus_tag="PLL14_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOG03007.1"
                     /translation="MELLINGESRQFPSPLTVADLVMALNHVGKRIAVERNGEIVARG
                     RHGETMLAEGDRIEIVVAVGGG"
     gene            3964..4872
                     /locus_tag="PLL14_03450"
     CDS             3964..4872
                     /locus_tag="PLL14_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOG03008.1"
                     /translation="MYPQRMHLLLGGGMLAWALLLAQAAAAASADAVVDAVQMPAWVE
                     RQGNREALQPGLVLRNRDHVLTGPDGRVRIRLGDGSVLRLGAETRLDLNALGIRESMF
                     TAAFDVPQGAVRFSTSTSARSHHQRAVNLRVGTITAGIRGTDVWGKADSAGDRICLLE
                     GSITVVHPDEEAQQISEASSCYQAAQESAATAIETVTAAQLSWWTQQTEMQSAPARAS
                     SPVMRGGKWTIELATLDSETAALDLYDRARAAGYLVRIRPQALSGGGYAYAVRLGQSM
                     NRPDAAALAEQVSQSLQIPSPFVVRH"
     gene            complement(4909..5256)
                     /locus_tag="PLL14_03455"
     CDS             complement(4909..5256)
                     /locus_tag="PLL14_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03009.1"
                     /translation="MKAPKILPWIAYKTGISEELALKLWRRAAGEAQVMTGCCDSSAY
                     YRLAVERFIDFAEAEGSRCVVRQSVGSRNCVSWLWRHQNRISKLNLITAENVVRLWQT
                     NLSNLFGVQRHAV"
BASE COUNT          964 a         1811 c         1680 g          955 t
ORIGIN      
        1 ttcatgctca acgccctgcg cctgattcag ggtttcgacg cgcaattgtt cgaatcacgc
       61 acctcgctgc cgctgttcag catcgagcac atcctgcgcc aggctcaggc ggacggcctg
      121 atcgagcgcg ccgggcaacg cattgcgccc agcgatcgtg gccgccgctt tctcaatcgg
      181 ctgctgcagc gcttccttgc agaccgcgac tgaccaggaa tcagcgcaac cgccgctgcg
      241 gctacggcta cggcttcatc gcttcgacca cctgatagag ttgctgccgg agtccgtcgg
      301 cgggctgccc ctgcgcctcg gcgaggcgaa tctgacgcag caaatccgcg gcctgtgcgg
      361 tcgccaggct gcgcgtttgc gccgcggcgt tgcggctcgg cgtcttcacg gcagcgctgc
      421 cgggtagtgg cgcgacgtcg ggcccggccg ggctgccacc caccagtaac tgcgcacgct
      481 cggccccggg ctgcgaacgg cgtgcggtaa tgaccatcga tcgcggttga tacgggcgcg
      541 gcatcgggcg ctgcaccacc ggccccgagc tgccgcgccg cagttcattg accgcggcga
      601 gaagtttctt gtgcaagtcc tcgctcggac ggccttcggc ttcagccgcg cggatttcct
      661 tcaggatcgc gtcgatcgcg ctgggcaccg ccgccgagcg gatcgcgtcg ccgaagttct
      721 ccttcggcac cttgaccgcg atcgcgaact cctccggcga cgaaatcatc tttgccagcc
      781 gcaagtggtc gtagcgcatc gccatggtca gcgccgtatc gccccagtcg ctctgcgcct
      841 tgggattggc gcccgcctca agcagggtcc gcgccagacc gtcgcgacct tcgcgcgcgg
      901 ccagcatcaa cggcgtcgag ccgttcggag aacgcgcatt gacgttggca ccgcgcgcca
      961 tcagatcatt gaccacctgt tcgtgcccgt tgaacaccgc gtagtgcagc gcgccccatt
     1021 gtttgccctc acgctccagc ggcgccccac gctccaggag ccacttgacg gcgtccagat
     1081 gtccgcccca ggccgccagc tgcaggggct gctcgccgtt gcggttgctg cgccggatgt
     1141 cggcaccgcg ttcgacgaac agcgccatca tttcgatgtt gccataccag gcggcgacca
     1201 tcagcccggt gccgatctgt gccgcctggt actcggggtt cagtccgtcg tcaagccagc
     1261 gtttgacggt gcgcaggtca cctcgctcga cggcataacc gaaggtgatc ggatccggtg
     1321 ccggcgcgtc tgccgacgcc ggaccaacca gcaaacaaca cacgagcaac atcacgccgc
     1381 caaatctgcc tatcaataga ctcattccgt cctccgtcca gacccgaccc ggcctgccga
     1441 gtcgcatcgc cgtgccctgg cgcacggttt tggttcacaa tgtcacgccc atgccgttac
     1501 gcctgatcct cgccctcgcg ctttcgctcg ccctgcacgc cggcctgctg cttccggacg
     1561 tctttggcca gcacaccccg ccacagcggt cggtgctggc ggcttcgctg cagctgtcgc
     1621 acggcccgca agaacccgac cgcgacgaga ccctgctgaa aaacacgcta ctgaccgaga
     1681 aggcaccggt caaagcgcca ccagctcccc cgcccgcggc ccacgccggg ccagcgcccg
     1741 gcaaagccgt gaccaagcgg caactcgacg cagtacagcg caagctggcg cagcatcagt
     1801 actatccgcc cgaagccatc gcccgcggca tcgaaggcga tggcagcctg ctgctgacgc
     1861 tggccgaaga cggcagcatc agcgaggtca gcatcgccgt cagcagcggc catccgattc
     1921 tcgacaaggc cgcagtcaga gcggcatacg cgatggccag ggttaactgg gcgcattcac
     1981 gcgaactgat cctgcctttc gtcttccggc tggagtgaat cctggcgtct gcgaaagacc
     2041 agatcccata ccccatgccc cagccgcagg ccacgctgct caaacttggt caaggggcga
     2101 tacgcggggc gaggcgcgta gtccggcgcc gtgttctgta gccgcggttc tgccgcaagc
     2161 acggccagca tctgttgagc gtagtcttcc cagtcggtgg cgcagtggat atagccatcc
     2221 ggctgcaggc ggctggccag cagcgccaca aagggtggct gcaacaggcg acgcttgtga
     2281 tgccgcttct tcggccaggg atcgggaaag aagacgtgca cgccggccag cgaagcgggc
     2341 gagatcatgt cgcgcacgac ttcgaccgca tcgtgttgaa caatgcgcag attgtgcaaa
     2401 gcccgctcgg cgaccagctt gcacaggctg ccgacgccgg gcgtgtgcac ttcgataccg
     2461 agatagtcgc gctcgggatg cagtgccgcg atcgccgccg aggtctcacc catgccgaag
     2521 cctatttcca gaatccgcgg cgcggctcgc ccaaacaagg tctcgaaatc gacacactgc
     2581 gcaacgtacg cgacgccgat cttcgccatc atgtcttcgt gataacgctg ctgggcgttc
     2641 gagacacgcc cctggcgacg cacgaaactg cggatgtgcg ccgcccgccc tggcgtgtac
     2701 gccgtctcct gcccggccac gcctacgaac cgatcaggcc ggtcgtcggc gacgaggggt
     2761 ccgcggagta gaacttgcgc ggcatgcgcc cggcgagaaa ggcttcgcgc cccgcctcga
     2821 ccgccttctt catcgcgctg gccatcagca cgggatcgcg ggcatgcgcg atcgcggtgt
     2881 tcatcagtac gccgtcgcac ccgagttcca tggcgatcgc cgcatccgac gcggtgccga
     2941 cgccggcatc gaccaacacc ggcaccttgg cgttatcgat gatcaggcgc agattccagg
     3001 gattgacgat gcccatcccg gaaccgatca gcgaagccag cggcatcacc gcgacgcagc
     3061 cgatctcctc cagcatcctg gcctggatcg ggtcgtcggc gcagtagacc atcacctcga
     3121 agccttcacg gaccagcgtt tccgccgcct tgagcgtttc cggcatgttc ggaaaaaggt
     3181 tgttcggatc acccagaact tcgagcttgc acagcgcgtg cccgtcgagc agttcgcgcg
     3241 ccagacgcaa ggtccgcacc gcgtccgccg cggtgtaaca gccggcggta ttcggcagga
     3301 tggtgaattg cgacggcggc aatacctcga gcaggttcgg ctcgccggca tcctgaccga
     3361 tgttggtgcg gcgaatggca acggtgacga tctctgcgcc ggaagcatcg attgccgccc
     3421 gcgttgccgt gaaatccggg tatttgccgg taccgacgag cagacgggaa cgataggtcc
     3481 tgccggcaac ggtcaggtgg tgtggggaag atggcatgtg tctttcgctt ttcggcttgt
     3541 caatgaggct cagccgccac cgacggcaac gacgatctcg atccggtccc cctcggcaag
     3601 catcgtttca ccatgccgac cgcgtgcgac gatctccccg ttgcgctcga cagcgatccg
     3661 cttgccgaca tgattcagcg ccattaccag atcggcgacc gtcagcggcg agggaaattg
     3721 gcggctctcg ccgttgatca ggagttccat gggaggagaa ttctagcacg ccgcgacagg
     3781 accaccgggt gcgcgtaaac cgcaaagcaa caagctgcgc aggactcaag atccgactgg
     3841 gcggcgcgga aaggtatcgt catgatttgc acgagtcggc ttacggtgaa caaggcttgc
     3901 aacaacgggc gcacttggtg cgatgttaca attgcccgat ctcactgtag ctagggattg
     3961 gccatgtatc cgcagcgcat gcaccttctc ctgggcggcg gtatgcttgc ctgggcttta
     4021 ctactcgcgc aggcagccgc tgcagcgagc gccgacgcag tcgtcgacgc ggtgcagatg
     4081 ccggcctggg tcgagcggca gggtaatcgc gaggcgctgc agccgggcct cgtgctgcgg
     4141 aatcgcgatc acgtgcttac cgggcccgat ggccgcgtgc ggattcgcct gggcgatggc
     4201 agcgtgctcc gcctgggggc agaaacccgc cttgacctga atgcgctcgg catccgcgaa
     4261 agcatgttta ccgccgcctt cgatgtcccc cagggtgcgg tgcgcttcag cacgagcacc
     4321 tccgcgcggt cgcatcacca gcgtgcggtc aatctgcgcg tggggaccat caccgccggc
     4381 attcgcggca ccgacgtgtg gggcaaagcc gactcggctg gggaccggat ctgccttctg
     4441 gagggcagca tcacggtggt ccaccctgat gaggaagcgc agcagatcag cgaggcttca
     4501 agctgctatc aagccgcgca ggaaagcgca gcgaccgcca ttgaaaccgt cacggcagcg
     4561 caactttcct ggtggacaca acagaccgaa atgcagagtg cgccagcacg ggccagcagc
     4621 ccggtcatgc gcggaggaaa atggacgatc gaactggcga cgctggatag tgaaacggcg
     4681 gctctggatc tgtacgaccg cgcgcgcgcc gccggctacc tggtgcgcat caggccgcag
     4741 gcactttccg gcgggggata tgcgtatgcg gtccgtctcg gccagtcgat gaaccggccc
     4801 gacgcggcgg cactcgcgga acaggtttcg cagtcactgc agatcccctc gccgttcgtg
     4861 gtgcgccact gagtgtgccg tcgacgctcg aggtcgccgc ggtagccttc agacagcgtg
     4921 ccgctgtacg ccgaagaggt tgctcaaatt ggtttgccac aatcgcacga cattctctgc
     4981 ggtgatcaga ttcagtttcg agatccggtt ctggtggcgc cacagccagg acacacagtt
     5041 cctgctgccg accgactggc gtaccacgca gcggctaccc tctgcttcgg caaaatcgat
     5101 aaagcgctcg acagccagac ggtaatacgc cgaactgtcg cagcaaccgg tcatgacctg
     5161 cgcttccccg gcggcgcgac gccacagctt gagtgcaagt tcttcgctga tgccggtctt
     5221 gtaggcaatc cacggcaata tttttggtgc tttcatgcta gtgctcctga gatgctggcg
     5281 acgccaggca ctgtaaccag ccggatgaaa cctcgttagc gggattttgt tgcagtgcag
     5341 catcattata gggaatatcc ggtggaaaag ttagggtttt cctctaaatt ttctccttcc
     5401 agccggtgat
//
LOCUS       DAOSIT010000092         5369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13659_length_5369_cov_9.626270, whole genome shotgun sequence.
ACCESSION   DAOSIT010000092 DAOSIT010000000
VERSION     DAOSIT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13659_length_5369_cov_9.626270"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /locus_tag="PLL14_03460"
     CDS             complement(<1..2177)
                     /locus_tag="PLL14_03460"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOG03010.1"
                     /translation="MVSVIHSVAAQSDFEQYQHMLAEGLHGAELARVPLAVDFARSIY
                     GDRRLGSGEHVWGHALGMALIVAGLKLDVDSRLAALLFAVPALQENGLAHIQKDFGVG
                     VTQLVDGISRLNRLRPITRGFAAASAESSEKNPQQHLAQIEVLRKMLLAMVEDIRVVL
                     LRLASRTQTLRYYAEEPDELRVPVARETLALYSPLANRLGVWELKWELEDLSFRFLHP
                     AIYKEIAQHLDEKRTEREQFITEASERLRAELLSAGVANAEIYGRPKHIYSIWNKMRK
                     KDVSFSEVYDVRAVRVIVDDVQACYTALGIVHHIWSPIAKEFDDYISHPKGNDYRSLH
                     TAVHCPDGRSLEVQIRTREMHSHAELGVAAHWRYKEGSKARPEDSYDEKIAWLRQLLT
                     WRDEVADSSDWVRQYKQAAFDETVYILTPQGRVVDLPRGATPVDFAYRVHTDLGHRCR
                     GAKVDGALVPLNTPLATGQRVEIILAKHGGPSRDWLNPSLTYLFTHRARLKVRQWFAS
                     LALEDTLAEGRAVVIRELQRMGQAGVNLDDLAAKLGFARSDDLFIAVARAKLNMRQLQ
                     VAVRGSETPVEERVPDELTVRRQRDSDASEKGILIVGIDRLLTQLAGCCKPAPPDPVV
                     GFVTRGKGVSVHRADCSNFSNMRSMHPERVIETTWGGRTDGVFAVDIVIDAHDRQGLL
                     RDVSDVLAREKINVIAVNTQSREGHAHMSFTAEVGNIPQLNRTL"
     gene            2243..3148
                     /locus_tag="PLL14_03465"
     CDS             2243..3148
                     /locus_tag="PLL14_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG03011.1"
                     /translation="MLSRLRHPYLLLVLTTLFWSGNMVLGRAIRDQVPPLSLAFWRWA
                     IALALVLPLALPHLRAQWPLLRAGWQAVVILGLLGVGGYNTLAYVGLQYTPATNAVLL
                     NSFIPIATITLSWLFLNKHLRALEWLGVLLSFAGVTAIVCRGDIATLAMLSPNIGDLW
                     MLAAVFTWALYTIGLQWRPVGLNPMLLLAAFTLVGLAALAPAYAWEIAQGKHIVISAG
                     SLAGIAYTGIFPGFLGYVFYNRAVAEVGASKASLFIHLMPVFGTILSALFLDEIPQIY
                     HYVGMTLIFAGIYLTTAARPQAKTP"
     gene            3145..>3831
                     /locus_tag="PLL14_03470"
     CDS             3145..>3831
                     /locus_tag="PLL14_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOG03012.1"
                     /translation="MIGRLLGWLRNRQEPAHVPDALWQSVVAALPFLVSLSAEEQFRL
                     RRLAEDFLASKEFTAAGGLALSDAICVSIAAQGCLPILNLGLEYYRGWVGVIVYADEF
                     IIPRSVEDEFGIVHEYQELASGEAWDGGPLLISWRDAQMAGSGYNVVIHEFAHKLDML
                     NGEANGIPPLPPEIARSDWQQVLRASYDDFCNLVDEADAAGRDTVFDPYASENPGEFF
                     AVMSEAFFETP"
     assembly_gap    3832..3931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3932..4587)
                     /locus_tag="PLL14_03475"
     CDS             complement(<3932..4587)
                     /locus_tag="PLL14_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG03013.1"
                     /translation="MAFVDVGGQSLEYCLIAPQRGDRSTLVFLHEGLGSVALWRDFPM
                     RVAQATGCRTLIWSRLGHGYSAPLPAPRTAAYLHDEALQRLPALLAALAIERPVLIGH
                     SDGGSIALIHAAAWPDRVAGVIVMAPHEFVEEKALDGIRRAGEAYARSDWRVKLARYH
                     RDPDVVFRAWHDTWLAPEFAGWQITDCLPAIRCPLLAMQGEDDEYATMRQIECIAEAA
                     P"
     gene            complement(4590..>5369)
                     /locus_tag="PLL14_03480"
     CDS             complement(4590..>5369)
                     /locus_tag="PLL14_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG03014.1"
                     /translation="EPENPNVLVCVHGLTRNGRDFDELARTLAADYRVICPDVVGRGR
                     SDWLRDSSGYSFPQYASDMMVLLARLNVASVHWLGTSMGGLIGMWIASQEDSPISRLV
                     LNDVGPLISVASLMRIGEYVGQAPKFDTYEDAEKYVRLVSQPFGPLSDAQWRHLTEFS
                     LQRLPDGRLAMRYDPAIGEAFRQALSAGDIDLWPIYDRIRCPTLVVRGAESDLLTRAT
                     LQAMAMRGPRPQTAEIAGVGHAPMFLDLQQIGIVRDFLLVV"
BASE COUNT          957 a         1743 c         1606 g          963 t
ORIGIN      
        1 agggtacgat tgagctgtgg gatgttgccg acttcggcgg tgaagctcat atgcgcgtgg
       61 ccttccctgg actgcgtatt gacggcgatc acgttgatct tctcgcgtgc cagaacgtcc
      121 gacacatcgc gtagcaggcc ctgccggtcg tgcgcatcga tcacgatatc gaccgcgaag
      181 acgccgtcgg ttcgcccgcc ccaggtcgtt tcgatgaccc gctcgggatg catcgagcgc
      241 atgttggaga agttgctgca atcggcgcga tgcaccgaaa cccccttgcc gcgcgtgacg
      301 aagcccacca cgggatcggg cggagcgggt ttgcagcagc ctgcgagctg ggtcaacaag
      361 cggtcgatgc cgacgatcag gatacccttc tcggaggcat cgctgtcgcg ctgccgacgc
      421 accgtcagtt cgtccggtac gcgctcctca accggtgtct cgctgccacg caccgccacc
      481 tgcaactggc gcatgttcag cttggcgcgg gccacggcaa tgaacaagtc gtcgcttctg
      541 gcaaaaccca gcttggccgc aagatcgtcc aggttgaccc cggcctgccc catccgctgc
      601 aattcgcgaa tcacgaccgc acgtccctcg gccagggtgt cttcgagagc caggctggca
      661 aaccattgcc tgaccttgag gcgtgcgcga tgcgtaaaca gatacgtcag cgaggggttc
      721 agccagtcgc gcgacggccc cccgtgcttg gcaagaatga tctcgacacg ctgtccggtg
      781 gccaatggcg tgttcagcgg caccagcgcg ccatcaacct tggcaccgcg gcagcggtgg
      841 ccgagatcgg tgtgcacacg gtacgcgaaa tcgaccggcg tcgcgccgcg cggcagatcg
      901 acgacccggc cttgcggggt caggatgtag accgtctcgt caaaggccgc ctgcttgtat
      961 tgccggaccc agtccgagga gtcggccact tcatcacgcc aggtcaacag ctggcgcagc
     1021 caggcgatct tttcgtcgta gctgtcttcg ggcctggcct tgctgccctc cttgtagcgc
     1081 cagtgcgcag caacacccag ttccgcgtgg ctatgcatct cgcgagtacg gatctgcact
     1141 tcgagcgagc gcccatcggg gcaatgcacg gccgtatgca gcgagcggta gtcattgcct
     1201 ttcgggtgtg aaatgtaatc gtcgaactcc ttggcaatcg gcgaccaaat gtggtgcacg
     1261 atccccagag cggtatagca ggcctgcaca tcgtcgacga tgacccgcac ggcgcgaaca
     1321 tcgtagactt cggaaaacga cacgtccttc ttgcgcatct tgttccagat gctgtagatg
     1381 tgttttggcc ggccgtagat ctcggcgttg gccacgccgg cgctcaacag ctcggcccgc
     1441 agccgttcgc tggcttcggt gatgaactgc tcgcgctcgg ttcgcttctc gtcgagatgt
     1501 tgcgcaatct ccttgtagat cgccggatgc aggaagcgga aagagaggtc ctcgagttcc
     1561 cacttcaatt cccagacgcc aagccggttg gccagcgggg aatacagcgc cagcgtttcg
     1621 cgcgctaccg ggacccgcaa ctcgtcgggc tcttcggcgt agtaacgcag ggtctgtgtg
     1681 cgcgaggcca gacgcaggag gacgacgcgg atatcctcga ccatcgccag cagcatcttg
     1741 cgcagaacct cgatctgcgc aagatgctgt tgcggattct tctcgctgct ctcggcggac
     1801 gcagcggcaa aaccgcgcgt gatcggtcgc aggcggttca ggcgcgaaat cccgtcgacc
     1861 agttgcgtga cgccgacccc aaaatccttc tgaatgtggg ccagaccgtt ttcctgcaat
     1921 gccggcacgg caaacagcag ggccgccagg cgcgaatcga catcgagctt gagcccggca
     1981 acgatcagcg ccattcccag cgcatgaccc cagacgtgct cgccgctgcc gagtcgccga
     2041 tcgccataga tcgagcgggc aaaatcgacg gccagtggaa cccgcgcgag ctcggcgcca
     2101 tgcagcccct cggccagcat gtgctggtat tgttcgaagt cgctctgcgc ggcgaccgag
     2161 tgaatgaccg aaaccatgga cgcattataa gggctcgcca gagcatcagg cataatgcgc
     2221 gattgcttcc cgcctgccgc cgatgctttc ccgccttcgc cacccctacc tgctgctcgt
     2281 gctgaccacc ctgttctggt ccggcaacat ggttctcggg cgcgccatac gcgaccaggt
     2341 ccccccgctc tcgctggctt tctggcgctg ggcgatcgct ctggctctgg tgctgccgct
     2401 cgcgctgccg cacctgcgcg cgcagtggcc gctgctgcgt gcgggctggc aagcggtggt
     2461 catcctggga ctgctcggcg tcggcggtta caacacgctg gcgtacgttg gcctgcaata
     2521 caccccggca accaacgccg tacttctcaa ttcgttcatc ccgatcgcca ccatcaccct
     2581 gtcgtggctc ttcctgaaca aacatctgcg cgcgctcgaa tggctcggcg tgttgctctc
     2641 gttcgccggg gtcacggcca ttgtctgccg tggcgacatc gccacactgg ccatgctgag
     2701 cccgaatatc ggcgacctgt ggatgctggc agcggtattc acctgggccc tgtacaccat
     2761 cggattgcag tggcgacccg ttggactcaa cccgatgctg ctgctcgccg ccttcacgct
     2821 ggtcggcctc gccgcactcg ccccggcata cgcctgggag atcgcccagg gcaagcacat
     2881 cgtcatttcg gccggatcgc tcgccggcat tgcctacacc ggaatttttc cggggtttct
     2941 cggctatgtg ttctataacc gcgcggttgc tgaagtcggc gccagtaaag ccagtttgtt
     3001 catccacctg atgccggtct tcggaaccat tctgtcggcc ctgttcctcg acgagatccc
     3061 gcagatatac cactacgtcg gtatgaccct gatcttcgct ggcatctacc tgaccaccgc
     3121 ggcgcggccg caggcaaaaa caccatgatc ggcaggctgc tcggctggtt gcggaatcgg
     3181 caggagccgg cgcacgtgcc cgatgcgcta tggcagtccg tggttgcggc gctgcccttc
     3241 ctggtttcac tcagtgccga agaacagttc cggctgcgcc ggctggccga ggatttcctc
     3301 gccagcaagg agttcaccgc cgccggcgga ctcgcactca gcgatgcgat ctgtgtttcg
     3361 attgccgcac agggttgcct gccgatcctc aatctcggcc tggaatacta ccgtggctgg
     3421 gtcggggtga tcgtgtacgc cgacgaattc atcatcccgc gcagcgtcga ggacgaattc
     3481 gggatagtcc acgagtatca ggaactggcg tcaggcgaag cctgggatgg cggaccattg
     3541 ctgatctcct ggcgcgacgc ccagatggcc ggcagcggct acaacgtcgt catccacgag
     3601 ttcgcccaca agctggacat gctcaatggc gaggcaaacg gcattccacc gctaccgccg
     3661 gagatcgcgc gcagcgactg gcagcaggtt ctgcgggcca gctacgatga tttctgcaac
     3721 ctggtcgacg aggcggatgc tgccggccgg gacaccgtat tcgaccccta cgcatcggag
     3781 aatccgggcg agtttttcgc ggtcatgagc gaggcctttt tcgagacacc gnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggggccgct tcggcgatgc actcgatctg
     3961 tcgcatcgtc gcgtattcat cgtcttcacc ctgcatcgcc aacagcggac aacggatcgc
     4021 cggcaggcag tcggtaatct gccagccagc gaattcgggc gccagccagg tgtcgtgcca
     4081 ggctcgaaaa acgacatccg ggtcccggtg gtaacgtgcg agcttcactc gccaatcgct
     4141 gcgcgcatag gcttcgcctg cgcggcggat gccatcgaga gccttctcct cgacaaactc
     4201 gtgcggggcc atgacgatca cgccggcgac ccgatccggc caggccgcgg cgtggatcaa
     4261 ggcgatcgag ccaccgtcgc tatggccgat cagcaccggt cgttcgatgg ccagcgcggc
     4321 gagcaaggct ggcaggcgct gcagcgcctc gtcgtgcagg tatgccgccg tccgcggcgc
     4381 cggcagcggc gccgagtagc cgtgcccgag gcgcgaccag atcagcgtgc gacaacccgt
     4441 cgcctgcgcc acccgcatcg ggaaatcacg ccacagggcg acgctgccga ggccctcatg
     4501 gagaaagacc agagtcgacc gatccccgcg ctggggcgca atcagacagt actcgagcga
     4561 ctgccccccg acgtcaacga aagccatcgt caaacgacga gcaggaagtc gcgcacgatg
     4621 ccgatctgct gcaggtcgag gaacatcgga gcatgcccga caccggcaat ttcagcggtc
     4681 tgcgggcgcg gcccgcgcat ggccatggcc tgcagcgttg cgcgcgtcag caggtccgac
     4741 tcagcgccgc gcaccaccag cgtcgggcag cggatacggt cgtagatcgg ccacagatcg
     4801 atgtctccag cactcagtgc ctgccgaaag gcttcgccga tcgcgggatc gtaacgcatc
     4861 gccagccgtc catccggaag acgctgcagg ctgaactcgg tcaggtggcg ccactgtgcg
     4921 tcgctcaggg gcccaaaggg ctgactgacc agacgcacat acttttccgc gtcttcgtag
     4981 gtgtcgaact ttggcgcttg cccaacgtat tcaccgatgc gcatcaagga cgccacactg
     5041 atcaacgggc cgacgtcgtt gagcacaagg cgcgaaattg gcgagtcttc ctggctggcg
     5101 atccacatcc cgatcaaacc gcccatcgaa gtacccagcc aatgcaccga ggcgacattc
     5161 aggcgagcca gcaacaccat catgtccgaa gcatactgtg ggaaggagta gccagacgaa
     5221 tcgcgcagcc agtcactgcg gccacgcccc acgacatcgg ggcagatgac gcgataatcc
     5281 gcggccagcg ttcgcgccag ctcgtcgaaa tcgcgcccat tgcgtgtcaa accatgtacg
     5341 caaaccagga cattcggatt ctccggctc
//
LOCUS       DAOSIT010000093         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13713_length_5352_cov_10.240136, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000093 DAOSIT010000000
VERSION     DAOSIT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13713_length_5352_cov_10.240136"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="glnE"
                     /locus_tag="PLL14_03485"
     CDS             complement(<1..1159)
                     /gene="glnE"
                     /locus_tag="PLL14_03485"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOG03015.1"
                     /translation="MNTTINGHAALPSALDSPWQAAQNHSHYLRQLLAARPEIAAWLD
                     EHAVLPVGARQMSDFLAQEAPGSEESLKRALRRLRQRVMATLIVRDIAGVAPLSEVVE
                     CMTQLADVTTNFALDFVHRQLGELFGEPLDSRGQPQRLLVIGMGKLGGRELNVSSDVD
                     YIFVYPEEGQTNGRRRIDNYDFFLRLGKRLIGALGDPTGDGQVFRVDMRLRPNGDSGA
                     LVGSLGSLENYFIAQGREWERYAWIKARVMNEGVNLQPEWVDGLQEICRPFVFRKYLD
                     FGAINAMRELHAQIRREVARKDMAEHIKLGPGGIREIEFIAQVFQLIRGGREPALQAR
                     PTLQILALLAERRLLPADSERELAVAYEFLRRLEHRLQYVNDAQTHRLPTGD"
     gene            1303..>1774
                     /locus_tag="PLL14_03490"
     CDS             1303..>1774
                     /locus_tag="PLL14_03490"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOG03016.1"
                     /translation="MPRPNLMRPEDLRPALYHRWHYLMPVISHPGVRLLGRAAVRGLV
                     ILYFGFVLLVLALRYLILPNIESYRPEIERYVSQTLGLTVAIGKIEASWQGINPDLIL
                     SDVRIADRQGQPALAFTRVESVLSWSSLPRLQIRLRLLRIDAPTLHLRRDADGQF"
     assembly_gap    1775..1874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1875..>3636
                     /locus_tag="PLL14_03495"
     CDS             <1875..>3636
                     /locus_tag="PLL14_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HOG03017.1"
                     /translation="LPHGRGAVRAWAGFADGKLSELTADLSLDDVNLRLARHLPALEL
                     DHMSGRIGARFSQGRVSVDGQRVELVTRRLHGESDAPLGNIHIEPTDFHVDWQRPAEG
                     SSLRGNFTASTLELAALAGLAAYLPLDTGSRQLLNDYAPRGRVSELRASFQGDAEGLQ
                     SYSLKSRFHDLALKPRDHMPGISGFSGSLDASEQGGTVQLRSQKVGFEFPDVLPESEI
                     ALETLTAQAKWKISKGRLDAELLRAEFAGPDAAGSAQGVYQRNGEGPGSIDLTAMLTR
                     ADARAVWRYLPAAINVDARHWVRDALKSGAASEARLALKGDLAKFPFLDRNQGQFLVT
                     VKAHDVTLDYGVGWPMITGIDADLRFEGVGMAVQARRGSILGARLSQTRAEIADFDAP
                     VSTLSVKGHAEGDTSEFLAFIKQSPVANQIDHFTDHMSASGKGRLDIALLIPLAEARL
                     GESKVEGTYTFLANEVNVDPALPPLRQLGGSLQFSEKDLRIPEINATLFGGPLKIKGG
                     VQGGKVLVIADGTVGMDEVRRRVEFPLLDQLSGTATYRAEIGVRKRNVELVLDSNLVG
                     VASALAAPFGKNASEAMPLH"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3737..4698
                     /locus_tag="PLL14_03500"
     CDS             <3737..4698
                     /locus_tag="PLL14_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOG03018.1"
                     /translation="GKLTARFKKWRRPDKAGSDAEPGEILKELPALDIVVDDFTVGAR
                     RFGRLDVQAHNEGGIWRLDRIELLNPYGSLSGSGQWQISNANRTQLDFTLDSSDVGKL
                     LDRLGYGGAVRGGSATLKGKIGWNGPPTELDYATLSGEMKLDASKGQFVKLDPGAGKL
                     LGLISLQGLPRRFSLDFGDVFSEGFGFDAIHGKMTVKSGLMHTDRLQIEGPAARVVMR
                     GDADLKHETQNLNVTVQPELGGSAALGIAVVNPIAGVATLLAHKLLQNPLNKVFSFEY
                     LVTGKWDDPKVEKISRSATPGSASPELPNPSNSTGASNESAQQ"
     gene            4679..>5352
                     /locus_tag="PLL14_03505"
     CDS             4679..>5352
                     /locus_tag="PLL14_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOG03019.1"
                     /translation="MNPPSNDLQYASQNRPRVRVAAVQMVSTPRVDENLRTAATLIAE
                     AVAGGAALVALPEYFPIMGISDADKVRVRECDGHGPIQDFLAASAREHAIWLIGGSLP
                     LSAEHPEKVLNSCLAYNPQGQRVARYDKIHLFGFRQGDECYDESATIEAGRQAVSFAT
                     PFGRVGLSICYDLRFPELYRALGVTDLLVIPAAFTETTGRAHWEILLRARAIENQCYV
                     LAVAQAG"
BASE COUNT          938 a         1612 c         1675 g          927 t
ORIGIN      
        1 cgtcgccggt cggcaggcgg tgcgtctggg catcattgac gtattgcagg cgatgttcga
       61 gtcggcggag aaactcgtag gccaccgcca gttcgcgctc gctgtcggca ggcagcaagc
      121 gacgctcggc gagcagggcg aggatctgca aggtcggccg tgcctgcagg gccggctcac
      181 gaccgccgcg tatcagttgg aagacctggg caatgaattc gatctcgcgt attccgccag
      241 ggcccagttt gatgtgctcg gccatgtcct tgcgcgccac ttcgcggcga atctgggcgt
      301 gcagttcgcg catggcgttg atcgcgccaa aatcgagata tttccggaaa acgaaggggc
      361 ggcagatctc ctgcaaaccg tcgacccatt cgggctgcag attgacgcct tcattcatca
      421 cacgcgcctt gatccaggcg taacgctccc attcacggcc ttgcgcgatg aagtagtttt
      481 cgagcgagcc gagcgagccg accagcgccc cggagtcgcc gttgggtcgc aggcgcatgt
      541 ccacccggaa aacctggccg tctcccgtgg ggtccccgag ggcgccgatc aggcgtttgc
      601 ccaggcgcag gaagaagtcg tagttgtcga tgcggcgacg cccgttggtt tgcccttcct
      661 ccggatagac aaagatgtag tcgacgtcgg acgacacatt gagctcccgt cctccgagtt
      721 tgcccatgcc gatgaccagc aaccgttgcg gctgaccgcg actgtcgagc ggctcgccga
      781 acaactcgcc cagttgccga tggacaaagt cgagcgcgaa gttggtcgtc acgtccgcga
      841 gttgagtcat gcattcgacg acttcgctca acggcgccac gcccgcaatg tcgcgaacga
      901 tcagcgtggc catcacgcgc tgacgaaggc gacgtagagc gcgcttcagg ctctcttcgc
      961 tgcccggcgc ttcctgtgcc aggaaatcgc tcatctgcct ggccccaacc ggcagcacgg
     1021 cgtgttcgtc gagccaggcc gcgatctccg gacgggcggc gagcagttgg cgcaggtagt
     1081 ggctgtggtt ctgggcagcc tgccagggtg aatccagagc actcggtaag gccgcgtggc
     1141 cattgatcgt ggtattcatt gaagtctcgc agggtggccg tcgaccgagg cgcgcccccg
     1201 gcacggaaga cccggggaaa gccgttacaa ttggcagctc gatcgtctgt ccaggtattc
     1261 cgacattcta gtgacgctga ccgactgttg gcaaaagtct ctgtgccccg gcccaatctg
     1321 atgcgcccgg aggacttgcg cccggcgctc taccatcgct ggcattacct gatgccggtg
     1381 atctcgcacc ccggcgttcg cctgctcggg cgggcggcgg tacgcggcct ggtgatcctc
     1441 tatttcggtt tcgtgctgct ggtcctggcc ttgcgctacc tgatcctgcc gaacatcgag
     1501 agctaccgac ccgaaatcga gcgctacgtc agccaaacgc tgggcctgac cgtcgccatc
     1561 gggaaaatcg aagccagttg gcaggggatc aaccccgatc tgatcctctc cgatgtgcgc
     1621 attgccgatc gacaggggca accggcgctg gcctttaccc gcgtcgagag cgtcctctct
     1681 tggtcgtcgc tgccacggct gcaaatacgt ctgcgcttgt tgcgcatcga cgcgccgacg
     1741 ctgcacctgc ggcgtgacgc cgacgggcaa ttctnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnccctcc cgcatggccg tggcgccgtg cgcgcctggg cgggttttgc
     1921 cgacggcaaa ctgtcggagc tgaccgccga cctctcgctc gacgatgtca atcttcgcct
     1981 ggcgaggcat ctgccggctc tggaactgga ccatatgtcc ggacgcattg gcgcacgctt
     2041 ttcgcagggg cgagtcagcg tcgacgggca acgggtcgaa ctcgtgacgc gcaggctcca
     2101 cggcgagagc gatgcccctc tggggaacat ccacatcgag ccaaccgatt tccacgtgga
     2161 ctggcagcgc ccggcagaag gcagctcact acgcggcaac ttcactgcca gcacactcga
     2221 actggcggcg ctcgccggcc ttgccgccta tctgccgctc gacaccgggt cacgccagtt
     2281 gttgaacgat tatgcgccgc gcggccgggt cagcgagttg cgcgcgagtt ttcagggtga
     2341 cgcggagggc ttgcagagct attcgctgaa aagccgtttc cacgatctgg cgctcaagcc
     2401 gagggaccac atgccaggga tcagcgggtt ttccggctct ctcgacgcca gtgagcaggg
     2461 cgggacggtg cagttgcgtt cgcagaaggt cgggttcgaa ttcccggacg tcctgccgga
     2521 gtcagagatt gcacttgaga ccctgactgc gcaagccaaa tggaaaatca gcaaggggcg
     2581 actcgacgcc gaactattgc gcgccgaatt tgccggacca gacgcggccg gctcggcgca
     2641 gggcgtgtac cagaggaatg gtgagggacc aggcagcatt gacctgacgg ccatgctgac
     2701 tcgtgccgac gcgcgcgcgg tctggcgcta cctgccggcg gcaatcaacg tggatgcccg
     2761 tcactgggtc cgtgatgccc tgaaaagcgg cgcggccagc gaggcgagac tggccctcaa
     2821 gggcgatctg gcaaagttcc ctttcctcga ccgcaatcag gggcagtttc tggtcaccgt
     2881 caaggcgcat gacgtgaccc tggattacgg cgtgggctgg ccgatgatca ccggcattga
     2941 cgccgacctt cgtttcgagg gcgtaggcat ggcggtacag gcccggcgcg gctcgattct
     3001 tggcgccagg ctatcgcaga cgcgcgcaga gatcgccgat ttcgatgcgc cggtgtcgac
     3061 gctgagtgtc aagggtcacg ccgagggcga cacgtcggaa ttcctggcgt tcatcaagca
     3121 gagtccggtg gcgaaccaga tcgatcactt caccgaccac atgagcgcca gcggcaaggg
     3181 ccgcctggac atcgcgctgc tcattcctct ggccgaggca cgactcggcg agtcgaaggt
     3241 cgaggggaca tacacctttc tggccaacga ggtgaacgtc gaccccgcgt tgccgcccct
     3301 gcgacaactc ggtggcagcc tgcagttctc ggagaaggat ttgcgcatac ccgagatcaa
     3361 cgccactttg ttcggcggac ccctcaagat caagggcggc gtgcagggcg gcaaggtgct
     3421 ggtgatcgcc gacggtactg tcggcatgga cgaggtgcgg cgacgcgtcg aattcccgct
     3481 tctcgaccag ctgtccggaa ccgccaccta ccgtgccgag ataggggtcc ggaaacgcaa
     3541 cgtcgagctg gtgctggact cgaatctggt cggcgtcgca tcggctctgg ctgcgccatt
     3601 cggcaagaac gccagcgaag cgatgcccct gcatttnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnccgg caagctgacg gcgcgcttca agaaatggcg acggccggac
     3781 aaggcgggca gcgatgccga gcccggcgag attctcaagg agttgcccgc gctggacatc
     3841 gtggtcgacg attttaccgt cggcgcccgt cgcttcggac gtctcgatgt ccaggcccat
     3901 aacgagggcg gtatctggcg gctggacagg atcgaactgc tcaaccctta cgggagcctg
     3961 tccggcagcg ggcagtggca gatcagcaat gccaatcgca cgcaactcga tttcacgctg
     4021 gacagcagtg atgtcggcaa attgctggac cgcctgggtt acggcggtgc ggtacgcggc
     4081 ggcagcgcga ccctgaaggg caagatcggc tggaacgggc cgccgaccga gcttgactac
     4141 gccaccttga gcggtgagat gaagcttgac gccagcaagg gccagttcgt gaagctcgat
     4201 cccggcgccg gcaagctgct cggcttgatc agcctgcagg ggctgccgcg tcggttttcg
     4261 cttgatttcg gcgacgtctt cagtgaaggc ttcggtttcg acgccatcca cggaaaaatg
     4321 accgtgaaga gcgggttgat gcacactgat cgtctgcaga tcgaagggcc ggcggcgcga
     4381 gtggtgatgc gcggcgacgc cgacctgaag cacgaaaccc agaacctcaa cgtaaccgtg
     4441 caacccgagc tcggtggcag cgccgcgctg ggcatcgctg tggtcaaccc aatagccggc
     4501 gttgccaccc tgctggcgca caaactgttg cagaatccgc tcaacaaggt cttcagcttc
     4561 gagtatctgg taaccggcaa gtgggacgac ccgaaagtcg agaagatttc cagatcggcc
     4621 actcccggga gcgcatcgcc cgaactgccc aatccttcca actcgaccgg agcgagcaat
     4681 gaatccgccc agcaatgatt tgcagtacgc gtcgcaaaac cgcccccgcg tccgggtggc
     4741 agcggtgcag atggtgtcaa cgccgcgcgt tgacgagaac ctgcgtaccg ctgcgaccct
     4801 gatcgccgag gcggtggcag ggggggctgc gctggtcgcc ctgcccgagt acttcccgat
     4861 catgggcatc agcgatgcgg acaaggtcag ggtgcgtgaa tgcgatggcc acggaccgat
     4921 tcaggatttt ctcgccgcca gcgcccgtga acacgcgatc tggctgatcg ggggttcgct
     4981 cccgctgtcg gccgagcacc ccgagaaggt gctcaattcc tgcctggcgt acaatccaca
     5041 agggcagcgc gtcgcgcgct acgacaagat ccatcttttc ggcttccggc agggtgacga
     5101 atgctacgac gagagtgcga ccatcgaggc cggccggcag gccgtcagct ttgccacgcc
     5161 gtttgggcgg gtcggcttgt caatctgcta cgacctcaga tttccggagc tttatcgagc
     5221 gctcggtgtc acagacctgc tggtgatacc tgccgccttc acggaaacga ccgggcgggc
     5281 acactgggaa atcctgttgc gggcgcgggc gatcgaaaac cagtgttatg tgctggccgt
     5341 ggcacaggcc gg
//
LOCUS       DAOSIT010000094         5324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13819_length_5324_cov_8.452648, whole genome shotgun sequence.
ACCESSION   DAOSIT010000094 DAOSIT010000000
VERSION     DAOSIT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13819_length_5324_cov_8.452648"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2240)
                     /locus_tag="PLL14_03510"
     CDS             complement(<1..2240)
                     /locus_tag="PLL14_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG03020.1"
                     /translation="MNGKLGEGQPAELSSEVSARLLRVVSVMVEETRPGRAERVHLHS
                     HLERELGLDSLARVELLLRVGSEFGKSLPDSALAEAETPQDLLQFIVRAGDDGFSAAT
                     VEFAPAAGPTSFPEQVRTLVEVLEWHAARNPQRLHVLLYGDGGGEQGAPESITYGGLL
                     EQARRVATGLVTHGLLPRQTVALMLPTGRDYLTSFFGVMLAGGIPVPIYPPARLAQIE
                     DHLRRHARILDNAQAVFIITVPQAKGVAALLRAEAPTLREVLTPEELDIEAISLLYRA
                     TADDIAFLQYTSGSTGDPKGVVLSHANLLANLRAMGEAVGVSSEDVFVSWLPLYHDMG
                     LIGAWFGSLYFGMRLVLMSPLAFLSRPVRWLRAISQHRGTLSPAPNFAYDLCARKLAD
                     ADLVGLDLSSWRLALNGAEPVSPATLEAFAERFSPYGLRAEAITPVYGLAECSVGLAF
                     PALGRGPRIDNIEREALVNRKCAIPAASGEADLVRVPACGRVLPRHEIRLVDEHGDEL
                     PDRRVGRLQFRGPSATVGYFRNPLATQALFRDGWLDSGDFAYTVDGEIYLTGRVKDLI
                     IRGGRNLYPYELEQAVGNLPGVRRGCVAVFASTDPANATERLVVLAETREQDPGQREA
                     LRQAINAAAVDAIGMPADEVVLAPPNSVLKTSSGKIRRNASREAFERGLIGASVVADR
                     QQLWRLGVASLRARLAESVERFGRWIFGVRCWTIFLLLAVPVMLSVLVVRAPTIGRRI
                     VHRAAR"
     gene            complement(2439..4610)
                     /gene="rnr"
                     /locus_tag="PLL14_03515"
     CDS             complement(2439..4610)
                     /gene="rnr"
                     /locus_tag="PLL14_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOG03021.1"
                     /translation="MSIMKLSAVRRRDPNLERELKRYENPLPSREYILQVLEEQGRPV
                     SFDELREILDIDKLELDTFQRRLRAMEREAQLLRNRKGSYILPERASLIAGRVEGHPD
                     GYGFLVPDDGSEDLALEARQMTKVLHRDRALVRVVGIDRCGRREGVIVEVLERANTRV
                     VGRVLIEHGITLVIPENRRINQDILVAPGGGGTAKSGQVVTVEIIEQPDRHSKPIGRI
                     VEVLGNYADPGMEIEIALRKHDLPFEFSSGTLAEANVLPETLAASDWEGRTDLRALPL
                     VTIDGETAKDFDDAVFAEKNGRGWRLLVAIADVSHYVQPGTALDRDAGERGNSVYFPR
                     RVIPMLPEKLSNGLCSLNPDVDRLVMVCDMAIDHHGQITDYRFYPAVFRSHARLTYDQ
                     VWNWLDGVTKPESDLHQRLLPHLQTLYALFQVLGEARGKRGAIDFETIETMMLFNAQG
                     KIENIVPVRRNDAHRLIEESMLAANVCASAFLQSNRQPCLYRIHQGPTPEKLEALRNF
                     LKEFGIGLGGGDDPTAKDYALLLDSIKTRPDAQLLQTVMLRSLRQAMYSPDNVGHFGL
                     SYEHYTHFTSPIRRYPDLLVHRSIKAVLAGTHYAPGKWDDIGLHCSMTERRADEATRD
                     VGNWLKCYYMRDRIGEVFAGTIAAVVPFGVFVALDEVYVEGLVHVSELGEDYFQYDST
                     KHQMLGERSGRRFRLGDRLQVKLVRADLETGRIDFVLSEAS"
     gene            4650..4736
                     /locus_tag="PLL14_03520"
     tRNA            4650..4736
                     /locus_tag="PLL14_03520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4684..4686,aa:Leu,seq:cag)
BASE COUNT         1065 a         1747 c         1574 g          938 t
ORIGIN      
        1 atccgggctg cgcgatgaac gatacggcgc ccgatcgtcg gcgcccgcac gaccaacaca
       61 gacagcatga ccggcaccgc cagaaggaga aagatcgtcc agcaccggac gccaaaaatc
      121 caccggccaa aacgttccac cgactcggcc aggcgcgcgc gcagcgacgc cacgccaaga
      181 cgccataact gctgtcggtc cgcgaccacg ctggcgccga tcaggccgcg ctcgaaggct
      241 tcacgtgatg cgttgcggcg gatcttgccg ctcgatgtct tgagcaccga attcggtggc
      301 gccagcacga cctcgtcagc cggcatgccg atggcgtcga ccgcagcagc gttgatcgcc
      361 tgccgcagcg cctcgcgttg tccgggatcc tgctcacgtg tctcggccag cacaaccagc
      421 cgctcggtgg cattggccgg atccgtgctg gcgaataccg cgacgcatcc gcgccggact
      481 cccggcaggt tgcctaccgc ttgctcgagt tcgtagggat agagattgcg accgccgcga
      541 atgatcagat ccttaaccct gccggtcaga tagatctcgc cgtcaacggt gtaggcgaaa
      601 tcgccggagt ccagccagcc atcgcggaaa agggcttgcg tcgccagcgg attgcggaaa
      661 tagcccaccg tcgccgacgg accgcgaaac tgcaagcgcc cgacgcgccg gtcgggcaac
      721 tcgtccccat gctcatcgac caggcggatc tcatgccgcg gcaggacccg accacaggcc
      781 ggcacgcgca ccagatcggc ttcgccgcta gcggcgggga tcgcgcactt gcggttgacc
      841 agggcttcgc gctcgatgtt gtcgatccgc gggccgcgtc ccagtgctgg aaaggccagc
      901 ccaaccgagc attcggcaag gccatacacc ggggtgatcg cctccgcacg caggccatac
      961 ggcgaaaaac gctcggcaaa ggcttccagg gtagccggac tcacgggctc ggcaccattg
     1021 agtgccagcc tccaggacga cagatcaagt cccacaaggt cagcgtcagc cagttttctc
     1081 gcgcacagat cgtaagcgaa gttcggtgcc ggtgaaagcg taccgcgatg ctgcgagatg
     1141 gcccgcagcc agcgcacggg acgcgaaaga aaggccagcg gcgacatcaa caccagacgc
     1201 atgccgaaat agagcgaccc gaaccaggcg ccgatcaagc ccatgtcatg atagagcggc
     1261 agccaggaaa cgaaaacgtc ctcgctggag actccgacag cctcgcccat ggcacgcaga
     1321 ttggccaaca ggttggcgtg gctgagtacc acgcccttgg gatcaccggt gctgcccgag
     1381 gtatattgaa ggaaggcaat gtcgtctgcg gtggcgcggt agagcaggct gatcgcctcg
     1441 atgtcgagtt cctccggggt caggacttcg cgcaacgtcg gtgcctcggc acgcagcagc
     1501 gcggcaaccc ctttcgcctg tggcacggtg atgatgaaca cggcctgggc gttgtccagg
     1561 atgcgtgcat gccgccgcag atgatcctcg atctgcgcca gccgtgccgg agggtagatc
     1621 gggaccggaa tgccgccggc cagcatcaca ccaaaaaagc tcgtcagata gtcgcgtccc
     1681 gtgggcaaca tcagggcaac cgtctgacgc ggcagcaggc cgtgtgtcac caggccggtg
     1741 gctactcggc gagcctgttc gagcagtccg ccataggtga tgctttccgg agcaccctgc
     1801 tcgcccccac catcgccata gagcaacacg tgtagacgct gcggattgcg cgcggcatgc
     1861 cattcgagga cttcgaccag cgtacgcacc tgctcgggaa agctggtcgg accggcggct
     1921 ggggcgaact cgacggtcgc cgccgaaaaa ccgtcatccc cggcccggac aatgaactgc
     1981 agcaggtcct gcggcgtctc tgcttcagcg agtgccgagt cgggtagcga cttgccgaac
     2041 tcgctgccca cgcgcaacag cagttcgacg cgcgccaggc tatccaggcc cagctcacgc
     2101 tccagatggc tgtgcaggtg cacccgttcg gcacgtcccg ggcgggtctc ctcgaccatt
     2161 accgacacca cccgcaagag tcttgccgaa acctcggacg atagctccgc cggctgacct
     2221 tcccccaact ttccgttcac tgtcgcaact ccctgtctcc caactggctg ccgcggatcg
     2281 tgtgcgaccg accccttcgc ctccggctac gccggtcggc agaccttcgg ggtaccgccg
     2341 tcagccttcc ggcgatcatc gaaccggcgc gagtctacca gactggccgc aactgcggcg
     2401 ctgggcacga gcacctacgt tccttcgcaa cgcatcgatc acgaagcttc ggagagcaca
     2461 aaatcaatcc gtccggtttc aagatcggct cggacaagct tcacctgcag gcggtcaccg
     2521 agacgaaagc gtctgccgct gcgctcgccg agcatctggt gcttggtact gtcgtattgg
     2581 aaataatctt cgccaagttc ggaaacgtgt accagaccct cgacatacac ctcgtcgagg
     2641 gcgacgaaga cgccgaaagg gaccaccgcc gcaatcgtcc cggcgaacac ttcgccaata
     2701 cggtcacgca tgtagtaaca cttgagccag ttgccgacat cgcgtgtcgc ttcatccgcg
     2761 cgccgttcgg tcatcgagca gtgcagcccg atgtcgtccc atttgccggg cgcgtagtgc
     2821 gtcccggcaa gcaccgcctt gatcgaacgg tgcacgagca ggtcgggata gcgacgtatc
     2881 ggcgaggtga aatgcgtgta gtgttcgtac gacaggccaa agtggccgac attgtcggga
     2941 ctgtacatgg cctgccgcag cgagcggagc atgaccgtct gcagcaactg cgcatcgggg
     3001 cgggtcttga tgctgtccag caacagggca taatccttgg cggtcggatc gtccccaccg
     3061 ccaagaccga tgccgaactc cttgaggaag ttgcgcagtg cctcgagttt ctccggcgtc
     3121 ggaccttgat gaatccggta caggcagggc tgccggttgc tctggagaaa cgccgaagca
     3181 cagacattgg cggccagcat gctctcctcg atcagtcggt gcgcgtcgtt gcggcgcacc
     3241 ggaacgatgt tctcgatctt gccctgcgcg ttgaacagca tcatcgtctc gatggtttcg
     3301 aagtcgatcg caccgcgctt gccgcgtgcc tcgccgagga cctggaacaa ggcgtagagc
     3361 gtctgcagat gcggcagcag acgctggtga agatcgctct ccggcttcgt cacaccgtcc
     3421 agccagttcc agacctggtc ataggtcagc cgcgcatgcg aacggaaaac tgccggatag
     3481 aaccggtagt cggtgatttg gccatggtgg tcgatggcca tgtcgcaaac catcaccaag
     3541 cggtcgacat cgggattcag cgaacacaga ccattggaaa gcttttccgg caacatcgga
     3601 atcacccggc gcgggaaata gaccgagttg ccgcgctcgc cggcgtcacg atcaagtgcc
     3661 gtacccggct gtacatagtg actgacgtcc gcgatggcca ccagcaagcg ccatccgcgt
     3721 ccatttttct ccgcgaaaac cgcatcatcg aaatcctttg ccgtttctcc gtcaatggtc
     3781 accagtggca gagcccgcaa atccgtgcgt ccttcccaat cgctggcggc caacgtctcc
     3841 ggcaagacat tggcttctgc aagcgtcccg ctcgagaact cgaatggcaa gtcatgcttg
     3901 cgcaaggcga tctcgatctc catgccaggg tctgcatagt tgccgagcac ctcgacaatg
     3961 cgcccgatcg gctttgaatg ccggtccggc tgttcgatga tctcgacggt caccacctgg
     4021 ccagatttgg ccgtgccgcc accgcccggc gcgaccagga tgtcctggtt gatacggcga
     4081 ttttccggga tcaccagcgt aatgccatgc tcaatcagca cccggccaac gaccctggta
     4141 ttggccctct ccaggacttc gacaatcacc ccctcgcgac gcccgcagcg atcgatcccg
     4201 accacgcgca ccagcgcgcg atcgcgatgc aggactttgg tcatctgacg agcctcgagg
     4261 gcgagatcct cgctaccatc atccgggacg aggaaaccat agccgtcggg atgtccctcg
     4321 acgcgcccgg caatcaggct ggcacgctcc ggaaggatgt acgagccttt gcggttgcgc
     4381 aacagttggg cttctcgctc catcgcccgc aagcggcgct ggaacgtgtc caactcaagc
     4441 ttgtcaatgt cgagaatttc acggagttcg tcgaaggaca ccggtcgccc ctgctcctcg
     4501 aggacctgca ggatatattc gcgggagggc aatggattct cgtagcgctt gagttcacgt
     4561 tccagattgg gatcgcggcg acgaacggcc gacagtttca ttattgacat tccttcatgt
     4621 tcctatagaa ttcggacttc gctggaaatg cccaggtggc gaaattggta gacgcgctag
     4681 gttcaggtcc tagtggtggc aacaccgtgg aggttcgatt cctctcttgg gcaccaagca
     4741 atgcacgaaa gccccgtttc tcggggcttt ttcgttttct gacgcccccc tcatggctgc
     4801 accgcacgca gcaggccggt gcaaaacccg cctgcactca ttgtacgcca tcacggctgc
     4861 cccctcctga cgaacaggca taactcccct cccggcattc cccgcaggcg accacatcgg
     4921 cccgcaccgc tggggcctcg tggctgccac ggcatgacct ggagccgggc gcttctccag
     4981 tcaagcgcta cggcgacgca tcgactggcc acagcctccg aaaatttttc tcactactag
     5041 ccaacgcacg tgaaaatatg gatactaccg tgccggaagg tcgccagcag cttactgctg
     5101 cgagaatgag aaccagttcc aacttagcca cctgttagga atccagatga cgatacgacc
     5161 ggagttgata gaaaagctgc gcgcgaggcg cgccaagctc accgaaaacg tcagtcccga
     5221 gaagttggag acgttgcacg ccaagggctt gttgtcggct cgcgagcgcc tcgacaccct
     5281 gtttgccgag ggcacgttcc aggaactcgg tctgcatgcc gcgc
//
LOCUS       DAOSIT010000095         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_13925_length_5294_cov_10.548196, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000095 DAOSIT010000000
VERSION     DAOSIT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13925_length_5294_cov_10.548196"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLL14_03525"
     CDS             complement(<1..186)
                     /locus_tag="PLL14_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG03022.1"
                     /translation="MIRPVLRMGDERLLEKARSVTDFGTPELCALITDMTETMHHLDG
                     AGLAAPQIGVGLRVVIFG"
     gene            complement(186..929)
                     /locus_tag="PLL14_03530"
     CDS             complement(186..929)
                     /locus_tag="PLL14_03530"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HOG03023.1"
                     /translation="MLAIIGGSGLSQFASLEVTQRQLVSTPYGEPSGTLTFGRVCGRP
                     VVFLARHGDEHTIPPHQVNYRANIWALRECGAAAIVAVAAVGSIRADLRPGDLVIPHQ
                     IIDYTWARQSTFHEGPDRAVRHVDFTEPYDPGLRRKLGDAAVAIGVEVSDSAVYATTQ
                     GPRLETAAEINRLERDGSDVVGMTGMPEAVLARELAVPYAAINVVANYAAGRGDSRTG
                     ISMEAIHEVLNEAMQQVQAIIETMLRADD"
     gene            complement(933..1481)
                     /locus_tag="PLL14_03535"
     CDS             complement(933..1481)
                     /locus_tag="PLL14_03535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HOG03024.1"
                     /translation="MIDPQQAMAILAAADIIHAADAVAAAVGRVACEISERLRDSNPV
                     LLCVLNGGVPFAGQLMTLLTFPLDFDYLHVTRYGQASSGGALLWRVEPSIALSGRTVL
                     VVDDILDEGVTLLAIRERVFQMGARACFTAVVADKLTGRRKPLRADFVALTVPDRFVF
                     GYGMDVRGAWRNLPAIYAVRED"
     gene            complement(1512..>3032)
                     /gene="ligA"
                     /locus_tag="PLL14_03540"
     CDS             complement(1512..>3032)
                     /gene="ligA"
                     /locus_tag="PLL14_03540"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG03025.1"
                     /translation="LEVRGEVLMWRSDFERMNARQREHGEKEFVNPRNAAAGSLRQLD
                     PRITARRPLRFFAYGVGSVAATGLPATHSVLLDRLAEWGFPVAQERRRVAGLAGLIAY
                     YVEMARRRESLPYAIDGVVYKVDDLAAQERLGFVSRAPRFAIAHKFPAEEAVTELLDI
                     SIQVGRTGALTPVARLAPVFVGGVTVTNATLHNEDEVLRKDLCIGDTVVVRRAGDVIP
                     EVVRVLPERRPAAGRRFVMPQTCPVCGSQVVRNPDEAVARCSGGLYCPAQRKQALLHF
                     ASRRALDIEGLGDKLVEQLVDSDIVRTPADLYRLGLLALANLERMAEKSAGNLLASIE
                     QSKQTTLARFIYALGIRNVGEATARDLARHFGSLDRLLAADEGQLLQVPDVGPIVAQC
                     LRQFFDEPHNLEVIEQLRAAGVAWHDGEPSAQTVSAVAGKVFVLTGTLPSLTRDAAKA
                     MLEAAGGKVTGSVSKKTDFVVAGAEAGSKLEKARLLGVKVIDEEQLLAILAGPAAP"
     assembly_gap    3033..3132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3230..4489)
                     /locus_tag="PLL14_03545"
     CDS             complement(3230..4489)
                     /locus_tag="PLL14_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HOG03026.1"
                     /translation="MTDLQMGLIGLGGAGVIGVLAYNKWQEHKHRKLAQELLSARQAD
                     VLLDEVLPEEMLAESAEVAPVAAGDVAAPSAVAVVTAGRSAPERVEPLLLRESVPDGA
                     GQEVGVTSGGSSASPPRSPAAAPEMPPVARPDELRSPVREAAAPLYLLSPTIDYIAAI
                     EVSEPAAAYRILEAQRASLARLGKPVNWIGYSEQSREWEAIVEDSDNAYRHIRVGLQL
                     VNRQGPVRDGDLSIFHLAMQDLATELMGVADLPLREPALQMAAQLDEFCAGVDIQIGV
                     NVISQGQVFPGTKVRALAESAGMVIDPEGRFARCDDDGNVLYVLANQETQGFSAESMR
                     TLTTHGMTFLLDVPRVAGGDRVLTQMVELARRFADALHGALVDDNRRPLSESAIEPIR
                     RQIVQYQAAMAARQLPAGGALVLRLFS"
     gene            complement(4498..>5294)
                     /locus_tag="PLL14_03550"
     CDS             complement(4498..>5294)
                     /locus_tag="PLL14_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOG03027.1"
                     /translation="RIGELKLKEQAATLTFEQFASQLAAANADEQSLQGELAGARLGS
                     LQNAIAALQRSIEALGAVNLAALDELEAARERKGYLDSQSEDLTQAMSTLENAIRRID
                     RETRDLLQTTYDAVNRSFGELFPTLFGGGEARLIMTGEEILDAGLQVMAQPPGKKNST
                     IHLLSGGEKALTAIALVFSMFQLNPAPFCLLDEVDAPLDDTNTERFCAMVRRMSANTQ
                     FLFISHNKITMEMAQQLVGVTMQESGVSRIVEVDMEEALRMREQLV"
BASE COUNT          977 a         1769 c         1548 g          900 t
ORIGIN      
        1 tccgaaaatc actacccgca ggccgacacc aatctgcggt gcagccagtc cggcaccatc
       61 gaggtggtgc atggtttctg tcatgtcggt aatcaacgcg cacagttccg gagtaccgaa
      121 gtccgtgacc gaccgagcct tttccaacag gcgctcgtcg cccattctca agactggtct
      181 aatcattagt catcagccct caacatcgtt tcaatgatcg cctgcacctg ctgcatcgcc
      241 tcattcagga cctcatgaat ggcttccata ctgatacccg tgcgactgtc gccccgccct
      301 gccgcgtagt tggcaacgac attgatcgca gcatagggaa cggccaactc gcgcgccagc
      361 accgcctcgg gcatgccggt catgcccacc acgtcggaac cgtcgcgctc cagccggttg
      421 atctcggcgg cggtttccag gcgcgggccc tgtgtggtcg catacaccgc cgaatcgctg
      481 acctccacgc cgatcgccac ggccgcatcg ccgagtttcc ggcgcaagcc tggatcgtag
      541 ggctcggtga agtcgacgtg cctgacggcg cgatccggac cttcgtgaaa ggtcgactga
      601 cgcgcccagg tatagtcgat gatctggtgc gggatcacca gatcgccagg ccggaggtcc
      661 gcacgaatgc tgccgaccgc cgccaccgcg acgatggcgg ccgcgccaca ctcccgcaag
      721 gcccagatgt tggcacgata gttcacctgg tgaggcggaa tcgtgtgttc gtcaccatga
      781 cgcgccagaa aaaccaccgg tcggccgcaa acccgaccaa aggtcaacgt cccggacggc
      841 tcgccgtatg gggtgctcac cagttggcgc tgcgtcacct caaggctggc gaactggctc
      901 aggccactgc cgccgatgat cgccagcatc tctcaatcct cccgcacggc gtagattgcc
      961 ggcaggttgc gccaggcacc gcgaacatcc atgccgtaac cgaagacgaa acgatcggga
     1021 accgtcagcg cgacaaaatc ggcccgcagc ggtttccgcc tgccggtcag cttgtcggct
     1081 accaccgccg tgaagcaggc ccgggcaccc atttggaaca cgcgttcacg aatcgccagc
     1141 agcgtcacgc cttcatcaag gatgtcgtca accaccagga ccgttctccc gctcagcgcg
     1201 atcgacggtt cgacacgcca caacagcgcg ccaccgctgc tggcctgccc gtaacgggta
     1261 acgtgcagat agtcgaagtc aagcgggaag gtcaacagcg tcatcaactg gccggcaaaa
     1321 ggcacgccgc cgttcaatac gcacaacagc accggattgc tgtcgcgcag tctttcactg
     1381 atctcgcacg cgacacggcc aaccgcggcg gcgaccgcgt ccgccgcgtg aataatatcg
     1441 gccgccgcga ggatggccat ggcctgttgc gggtcaatca tgcattctcc ggatcgcagg
     1501 ttccagcggg ttcaaggcgc ggctggcccg gccaatattg caagaagctg ctcttcgtcg
     1561 atgaccttga ccccgagaag tcgagccttt tcgagcttgc tgccggcctc ggcaccggcg
     1621 acgacgaaat cggtcttctt cgacacggaa ccagtcacct tgccgccggc agcttcgagc
     1681 atggccttgg cggcatcgcg agtcagagaa gggagcgtgc cggtcaatac gaaaaccttg
     1741 ccggcaaccg cactgacggt ctgcgccgac ggctcgccgt cgtgccatgc cacgccggcc
     1801 gcgcgtaatt gctcgatgac ctcgaggtta tgcggctcgt caaagaactg tcgaagacat
     1861 tgcgcgacga tcgggccgac atccggcacc tgcagcaact gcccttcgtc ggcagccagc
     1921 aggcggtcga ggctgccaaa atgacgcgcc agatcgcggg cagtggcttc tccgacgttg
     1981 cgaatcccca gggcgtaaat gaaccgcgcc agcgtcgtct gcttgctctg ttcgattgac
     2041 gccagcagat tgccggccga cttctcggcc atccgctcga gattggccag tgccagcagc
     2101 cccagccggt agaggtcggc cggggtgcgc acgatgtcgc tgtcaacgag ttgctcgacg
     2161 agcttgtcac ccagcccttc gatgtccagt gcccgtcgcg aggcgaagtg cagcagtgcc
     2221 tgcttgcgct gcgccggaca gtagaggcca ccgctgcagc gggccaccgc ctcgtccggg
     2281 ttgcgaacca cctgcgaacc acagaccggg caggtttgcg gcatgacgaa tcgccgcccg
     2341 gcggcaggcc gcctttcagg caaaacgcga acgacctccg ggatcacatc gccggcccgg
     2401 cgaacgacaa ccgtatcgcc aatgcacaag tccttgcgca acacctcgtc ctcgttgtgc
     2461 agggtggcgt tggtcaccgt gacgccaccg acaaaaaccg gcgccagccg cgccaccggc
     2521 gtcagcgcac cggtccgacc cacctggata gagatatcga gcagttcggt gacggcttcc
     2581 tcggccggaa acttgtgcgc aatcgcaaaa cgcggggcac gcgaaacgaa gccaaggcgt
     2641 tcctgcgcgg ccagatcgtc gaccttgtag actacgccgt cgatggcgta gggcagtgac
     2701 tcgcgccgcc gggccatctc gacgtagtag gcgatcaggc cagccagccc ggccacccga
     2761 cgccgctcct gcgctaccgg gaaaccccat tcggcaagcc ggtcgagcaa gaccgagtgc
     2821 gtcgccggca gaccggtggc agcgacgctg ccaacgccgt aagcaaaaaa gcgcagtggc
     2881 cgccgggcgg tgatgcgcgg gtcgagttgg cgcaggctgc cggccgcggc attgcgcgga
     2941 ttgacgaact ccttctcgcc atgctcgcgc tggcgcgcat tcatgcgctc gaaatcgctg
     3001 cgccacatca gcacctcgcc gcgcacctcg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnggcgcgtc aaggacgtag tactgccgat tgtgtcgttc aatctccgca
     3181 cgcaaagtcc ggatgcgctc gctgacgctg atcaccgaat atcctgccgt caggagaaca
     3241 gccgcagcac cagtgcccca ccggccggca attgacgtgc ggccatcgcc gcctggtatt
     3301 gcacgatctg acgccggatc ggttcgatcg cactctccga cagcggacgc cggttatcgt
     3361 ccaccaatgc accatgcagc gcatctgcga accgccgcgc caactcgacc atctgggtca
     3421 acacgcgatc gccaccggca acccgcggca cgtcgagcag aaaagtcatg ccgtgtgtcg
     3481 tcagggtgcg catcgactcg gccgagaatc cctgcgtttc ctggttggcc aggacgtaca
     3541 gcacgttgcc gtcgtcgtca caacgggcaa agcgcccttc cgggtcgatc accatgccgg
     3601 ctgactcggc cagcgcgcgc accttggtac ccggaaagac ctgtccctgg ctgatcacgt
     3661 tgacgccgat ctgaatgtcc acaccggcgc agaactcgtc aagttgcgcc gccatctgca
     3721 aagccggttc gcgcagcggc aagtcggcga ctcccatcaa ttcggtcgcc agatcctgca
     3781 tcgcgaggtg aaagatcgac aggtcaccgt cgcgaaccgg accctggcgg ttgaccagtt
     3841 gcagcccgac acgaatatga cggtacgcgt tgtcgctgtc ttcgacgatc gcttcccatt
     3901 cgcgcgactg ttcgctgtag ccgatccagt taacgggttt tcccaggcgc gccagcgaag
     3961 cgcgctgcgc ttccaggatc cgataggcgg cggccggctc ggaaacctcg atcgcggcaa
     4021 tgtaatcgat cgttggcgac aacaggtaga gtggcgcggc ggcctccctg accggcgacc
     4081 gcagttcgtc ggggcgcgca accggcggca tctcgggcgc tgctgccggt gagcgtggcg
     4141 gggacgcgct gctccctccg gaggtcactc cgacttcctg tccggcacca tccggtacgc
     4201 tctcccgcag caacagcggt tcgacacgct ccggcgcgga gcgacccgcc gtcaccacgg
     4261 ccacggcaga cggcgcggcg acatcacccg ccgctacggg cgccacttct gccgattccg
     4321 ccagcatctc ctccggcaac acctcgtcga ggaggacgtc ggcctggcgg gcgctcaaca
     4381 actcctgcgc cagtttgcgg tgcttgtgtt cctgccactt gttgtaggcc agcacgccaa
     4441 tcacgccggc accaccgagg ccgatcaaac ccatctgcaa gtcggtcatt tccattccta
     4501 aacgagttgc tcgcgcatgc gcagcgcttc ttccatatcg acttcgacga tgcgcgagac
     4561 gccggattcc tgcatggtca ccccgacgag ctgttgcgcc atctccatcg tgatcttgtt
     4621 atggctgatg aacaggaact gcgtattcgc cgacatgcgt cgcaccatcg cgcagaaccg
     4681 ttcggtattg gtatcgtcca gtggcgcatc gacctcgtca agcaggcaga acggtgccgg
     4741 attgagctga aacatcgaga ataccagcgc aatggcggtc aacgccttct cgccgccgga
     4801 aagcaggtga atcgtcgagt tcttcttgcc cggtggctgc gccatgacct gcagaccggc
     4861 atcgagaatc tcttcaccgg tcatgatcaa gcgggcctcg ccaccgccga agagcgtcgg
     4921 aaacagttcg ccgaaactac ggttgaccgc gtcgtaagtg gtctgcaaca gatcccgcgt
     4981 ttcgcggtca atgcggcgaa tcgcgttctc cagcgtggac atcgcctgcg tcaggtcttc
     5041 cgactggcta tcgagataac ccttgcgttc acgcgccgct tcgagttcat cgagggccgc
     5101 cagattgacc gcaccgagcg cttcaatcga gcgctgcagc gcggcaatcg cattctgcag
     5161 gctgcccagg cgtgcgccgg cgagttcacc ctgcagcgac tgttcgtcgg cattggccgc
     5221 cgccagttgc gaggcgaact gctcgaaggt gagcgtggcg gcctgttcct tgagtttcag
     5281 ttcgccaatc cggt
//
LOCUS       DAOSIT010000096         5252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14060_length_5252_cov_7.494708, whole genome shotgun sequence.
ACCESSION   DAOSIT010000096 DAOSIT010000000
VERSION     DAOSIT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_5252_cov_7.494708"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLL14_03555"
     CDS             <1..555
                     /locus_tag="PLL14_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOG03028.1"
                     /translation="PIQSYAHLRLHNGTIWRWNRMLIGFDDNDQPHLRVEQRVMPAGP
                     SIVDMIANAALYYGAVSMLARQKVAPESRLPFAVARENFYRAARAGLGARLVWFDGRE
                     TTAVELLERQLLPLAREGLMRLELDAADVARYLQVIAGRLRSRQNGAAWQLAHYAKHR
                     DFFRLTADYLEQQRRLVPVHEWPL"
     gene            569..1612
                     /locus_tag="PLL14_03560"
     CDS             569..1612
                     /locus_tag="PLL14_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOG03029.1"
                     /translation="MLTVLDTLPAHFLDTPARELHRLLPGPTLIHLPGRRQPPLFVSV
                     LLHGNEDSGVVALQSVLRTYAGRPLPRALSILVGNVAAAAEGLRRLDNQPDYNRVWPG
                     GEAYAGSRECAAMGEVHRMMQARGVFASIDVHNNTGINPHYCVVNRLDQAVLHLALLF
                     SRTVVWFRGLAGTQTTAFSPFCPSLTVECGKPGKSTNEEHAARFIEACLHLATFPVHA
                     VRGDDIDLYHTVATVRVPTAVSFGFGDARADVDFDPQLDHMNFRQLDPGTVFGRTQLE
                     LPIDVRDESGCDIAHEFFACGGGTIRLRRAATPAMLTLDARVVRQDCLCYLMERLPLP
                     RHENAARELCASL"
     gene            1666..>2270
                     /locus_tag="PLL14_03565"
     CDS             1666..>2270
                     /locus_tag="PLL14_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03030.1"
                     /translation="MTWKCGVLRTSRPWRAILVVLIAAFLPGSAVLADSSGEQLAAYY
                     DRHMAVRGGIAYGWIGSDRPTPMISDVVQVGVSKDAYYALRRDGSLLTWTASPASATL
                     LMRDVLSFAAGNSGWLAINRTRSLWQGSGREPPRKIAERVIAAAVGDGTDYYITQAGE
                     LYVRGLANRGQYGDGRLLAAGEFVKTASQVVAVRAHTGHAL"
     assembly_gap    2271..2370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2481..>3658)
                     /locus_tag="PLL14_03570"
     CDS             complement(2481..>3658)
                     /locus_tag="PLL14_03570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG03031.1"
                     /translation="LARIRRLIEDLLPALGSEDEWLFGQARTLLQTLTGPAVDIQTLL
                     GLLTGFSQRASLAAEEQVEIKELLLKLLHLIIENIGELTLDQQCIQGQIDSLVRAVAP
                     PLSLRRLDEAERRLQEVISRQHEAKSRTLEAQEAMCEMLSAFIDQLTAMNQSSTDFQN
                     NLEQSASEISEVHTLEDLTPLLQRVIDATRAMAQDTATARDQLEELQDKVLTTDAELA
                     KLHEELHHASASARHDPLTDALNRRGLAEALAREIASMQRKDSPLCISLLDVDNFKKI
                     NDRLGHATGDEALVHLVNVVRHYMRPIDTLARYGGEEFVILMPDTRQEEGIETMKRLQ
                     RELTKNFFLTGNERILITFSAGVTQLTSDESGEEAIRRADQAMYLAKRTGKNRVLGG"
     assembly_gap    3659..3758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3759..4708
                     /locus_tag="PLL14_03575"
     CDS             <3759..4708
                     /locus_tag="PLL14_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOG03032.1"
                     /translation="VDDVLRDVLTRLGAYDLAVSEFLRVSGSVLPVRSFHRICPELGR
                     GSRTDAGTPVVVQLLGSDPACLADNAAQLVELSPAGIDLNFGCPAPTVNRHGGGATLL
                     TDPEQLFRIVATVRRVVPAEIPFTAKMRLGVRDTARTLDCARALAAGGVVSLVVHART
                     RDEGYRPPAHWEWVARIKDVVQQPVTANGEVWTTADHARCCAVSGTQDVMLGRGAVAD
                     PFLARRIRALAAGQAIERDRSLDWLLLQPLLASYWQQVCGKVSPRQAPGRLKLWLGSL
                     RRNFIEAEALFIAVRALRGVEEVSRVLQRHGVPVVPAAG"
     gene            4719..>5252
                     /locus_tag="PLL14_03580"
     CDS             4719..>5252
                     /locus_tag="PLL14_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG03033.1"
                     /translation="MFGNSRVPDSAQDDIHPHLATLLDRHAGAPFRKPYADYNRAAFA
                     ASLERWQRTAAGSAVILDSGCGVGESSLSLARNFPDHYVIGVDQSASRLLRGKRGVAQ
                     WPPNLDLVRADVVDYWRLLRAAGIRLDRHYLLYPNPWPKASQVSRRWHGHPVFPSLLD
                     LGGVLECRSNWAIYLAEF"
BASE COUNT          783 a         1568 c         1681 g         1020 t
ORIGIN      
        1 ccgatacagt cctacgccca tctgcgcctg cacaacggaa ccatctggcg ctggaatcgc
       61 atgctgatcg gcttcgacga taatgatcag ccgcacttgc gggtcgagca gcgggtgatg
      121 cccgccggac ccagcatcgt cgacatgatc gccaatgcag cgttgtatta cggcgcggtc
      181 agcatgctgg cccggcagaa ggtggcacca gaatcgcggt tgcccttcgc ggtcgcacgc
      241 gagaacttct accgggctgc gcgagcgggt cttggggcgc gcctggtctg gttcgacggt
      301 cgggagacaa ccgccgtcga actgctcgaa cgccagttgt tgccgctggc gcgcgaaggc
      361 ttgatgcggc tcgaactcga cgccgccgac gtcgcgcggt acctgcaggt gatcgccgga
      421 cgtttgcgta gccgtcagaa cggggcggcc tggcaactgg cgcactacgc gaaacaccgc
      481 gactttttcc ggctcaccgc cgactatctt gagcaacagc gccgtctcgt gcccgtgcat
      541 gagtggcccc tatgagcgtt ttcccttcat gctgaccgta ctcgacaccc tgccggcaca
      601 ctttctcgat acccctgccc gcgaactcca ccgtctgctg cccggtccga cgctgattca
      661 tctgcctgga cgtcgccagc cgccgctgtt cgtctcggtg ctgctgcacg gcaacgaaga
      721 cagcggcgtg gtggccctgc agagcgtgtt gcggacatac gccggtcgcc cgctgccgcg
      781 agccctgtcg atcctggtcg gcaatgtagc agcggcggca gaggggctgc gccgtcttga
      841 taatcagccc gactacaacc gggtctggcc gggtggcgag gcgtacgccg gttcgcggga
      901 atgtgccgcg atgggcgagg tgcatcgcat gatgcaggcg cgcggtgtct ttgccagcat
      961 cgacgtgcac aacaacaccg gcatcaatcc gcattactgc gtggtgaatc gtcttgatca
     1021 ggccgtgttg cacctggcgc tcctgttttc ccgcaccgtc gtctggtttc gcggcctggc
     1081 gggcacgcag acgacggcgt tttcgccgtt ttgcccctca ttgaccgtgg aatgcggcaa
     1141 gcccggaaaa tcgaccaacg aggaacacgc ggcgcgtttc atcgaggcct gtctgcatct
     1201 ggcaactttc cctgtacacg ctgtgcgtgg ggacgacatc gatctctacc atacggtagc
     1261 gacggtgcgc gtaccgaccg cggtatcgtt cggctttggc gatgcgcgcg cagacgtcga
     1321 tttcgatccg caactcgacc acatgaattt ccggcaactc gatcccggca cggtgttcgg
     1381 acggacgcaa ctggaactgc cgatcgacgt ccgcgatgaa agcgggtgtg acatagcgca
     1441 cgagttcttt gcctgcggcg ggggtaccat ccgcctgcgg cgcgcggcaa cgccggcgat
     1501 gctgactctc gatgcgcgcg tggtgcgcca ggactgcctc tgttacctga tggagcgatt
     1561 gcccttgccg cgacacgaaa atgccgccag ggaattgtgc gcaagcttgt aggagatgag
     1621 gagaacgcgg tgtctggtga tggcgtggag ggcaggcagc acactttgac ctggaagtgc
     1681 ggtgtactcc gcacgtccag gccctggcgg gcgattctgg ttgtgctcat cgcggccttc
     1741 ctgccaggct cggcagttct ggcggacagt tccggcgaac aactggcggc ctactacgac
     1801 cggcacatgg cggtcagggg cggtattgcc tatggctgga tcggcagcga tcgaccgacg
     1861 ccgatgattt ccgatgtggt tcaggtgggc gtgagcaagg acgcctacta tgccctgcgc
     1921 agggacggca gcctgctgac ctggacggca tcgccggcca gcgctacctt gctgatgcgc
     1981 gacgtgctca gcttcgccgc cggcaattcg ggttggctgg cgatcaaccg gacccgcagc
     2041 ctatggcagg gcagtggcag ggagccgccg cgcaagattg ccgaaagagt catcgccgcc
     2101 gcggtcggtg acggaaccga ctactacatt acgcaggctg gcgaactcta tgtccgcggc
     2161 ctggccaatc ggggtcagta tggcgatggt cgtctgctgg cggccggcga gttcgtcaag
     2221 accgcgtcgc aggttgttgc cgtcagggcg cataccggtc acgcgctgta nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccgacggca agctttgggc ctgggatggt
     2401 ggccgcgggc ctcgccagat cgttctcgat cgctgaccgg gggtctgggg cgcaggcggt
     2461 gagacctgca tcggcaaatc ctagccgccc aggacgcggt tctttccggt tcgtttggcc
     2521 aggtacatgg cctggtcagc ccgtctgatc gcctcttcgc cagactcgtc cgacgtcaac
     2581 tgggtgaccc cggcgctgaa ggtaatcagg atcctttcat tgccggtcag gaagaagttc
     2641 ttggtgagtt ctcgctgcag gcgcttcatc gtctcgatgc cctcttcctg gcgggtatcc
     2701 ggcatcaata tgacgaattc ctcgccgccg tagcgtgcca gggtgtcgat cggacgcatg
     2761 tagtggcgca cgacattgac cagatggaca agcgcctcgt cgcccgttgc gtggcccagc
     2821 cggtcattga tcttcttgaa attgtcgacg tcgagcaggg aaatgcaaag gggggaatcc
     2881 ttgcgttgca tgctggcgat ttcgcgcgcc agcgcctcgg ctagccctct gcggttcagc
     2941 gcgtcggtca aggggtcgtg gcgggcagaa gcgctcgcgt ggtgcagttc ctcgtgcagc
     3001 ttggcgagtt cggcgtcggt ggtgaggacc ttgtcctgga gttcttccag ttgatcacgg
     3061 gcagtcgcgg tatcctgggc catcgcgcgg gtggcatcga tcacccgttg cagcagcggt
     3121 gtcaggtctt cgagcgtgtg cacttcggaa atctcgctgg cgctctgttc gaggttgttc
     3181 tggaagtcgg tgctggactg attcatcgcc gtcagttgat cgataaatgc cgaaagcatt
     3241 tcgcacattg cttcctgggc ttccagtgtc cggctctttg cttcgtgttg tctggatatg
     3301 acctcctgca ggcgtcgctc ggcctcgtcc agtcgacgca aactgagagg cggggccacc
     3361 gcccggacca gcgagtcgat ctgcccctgg atgcattgct ggtccagggt cagttcgccg
     3421 atgttttcga tgatcagatg cagcagcttg agcaggagtt ccttgatttc cacctgctct
     3481 tctgcggcca gcgatgctcg ttggctgaaa ccggtcagca ggcccagcag tgtttggatg
     3541 tccaccgccg ggcctgtcag cgtttgcagc agcgtgcgcg cctggccgaa caaccactcg
     3601 tcttcgctgc ccagcgcggg cagcagatcc tcgatcaggc gccggatcct ggccagcann
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gtcgatgacg tcttgcgcga
     3781 cgtgttgacg cgtctgggcg cctacgatct ggcggtgagc gagtttctgc gtgtttcggg
     3841 cagcgtgctg ccggtacgaa gctttcaccg aatctgcccc gaactcggcc gcggcagccg
     3901 caccgacgcc ggcacaccgg tggtcgtcca gcttctcggc agtgacccgg cctgtctggc
     3961 cgacaacgcg gcgcagttgg tcgaactctc gccggccggg atcgacctga acttcgggtg
     4021 cccagcgccg acggtgaatc gccacggcgg tggtgcgacg ctgctcactg atccggagca
     4081 attgtttcgc atcgtcgcta cggtacggcg cgtggtgccc gccgaaatcc cattcaccgc
     4141 caagatgcga ctcggcgtgc gcgacacggc gaggaccctc gactgtgcgc gggctctggc
     4201 cgccggagga gtcgtgtcgc tggtcgtgca tgcgcgtact cgggacgaag gctaccggcc
     4261 gccggctcac tgggaatggg tggcgcgcat caaggatgtc gtgcagcagc cggtgactgc
     4321 caacggtgaa gtctggacga cggcggacca tgcccgttgc tgcgcagtca gtggcactca
     4381 ggatgtgatg ctcggtcgcg gggcggtcgc cgatccgttt cttgcccggc gcattcgcgc
     4441 gctggcggcg ggtcaggcga tcgagcgcga tcgctcgctg gattggctgc tgttgcagcc
     4501 cttgctggcg tcttactggc aacaggtctg tggcaaggtt tcgccgcgac aggcgccggg
     4561 tcgcctcaaa ttgtggctgg gatcgctgcg gcgcaatttt atcgaagccg aagcgctgtt
     4621 catcgccgtg cgtgcgctgc gcggggtcga agaagtctcg cgcgtgctcc agaggcatgg
     4681 cgttcccgtc gtgccggcgg ctggctgagc cgcgctcgat gttcggcaac tcgcgcgttc
     4741 ccgatagcgc gcaggacgac attcacccgc atctggcgac cctgctcgac cggcatgctg
     4801 gcgcaccttt ccgcaagccc tatgccgact acaatcgtgc ggcgtttgcg gccagcctcg
     4861 aacgttggca gcgcacggct gccggatcag cggtcattct cgattcaggc tgtggcgtgg
     4921 gggagagcag tctgtctttg gccaggaact tccccgacca ctacgtgatc ggcgttgatc
     4981 agtcggcgtc gcgcctgctg cgcggcaagc gtggcgttgc gcagtggccg ccgaatctcg
     5041 atctggtgcg cgccgatgtg gtcgactact ggcgtctgct gcgcgcggct ggcattcggc
     5101 tcgaccgaca ctatctgctt tacccgaacc cctggccgaa ggccagccag gtgagtcgcc
     5161 gctggcacgg gcacccggtc tttccgtccc tgctcgacct gggcggggtg ctggagtgcc
     5221 gcagcaactg ggcaatctac ctggcggagt tc
//
LOCUS       DAOSIT010000097         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14064_length_5251_cov_9.622017, whole genome shotgun sequence.
ACCESSION   DAOSIT010000097 DAOSIT010000000
VERSION     DAOSIT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14064_length_5251_cov_9.622017"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PLL14_03585"
     CDS             complement(<1..1225)
                     /locus_tag="PLL14_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG03034.1"
                     /translation="MPTRPETSLPERPLLLIVDDDALICDTLSFSMRPLFEVVTSHSR
                     PHCMQLLRELRRAPSLALVDLGLPPVPHRPDEGLALISDLLKAAPEIRIVVLSGQSDE
                     GNARHARALGAADFVAKPCNPADLQQVLKRALAFRALDERQHQADGTSPLIGSSPAIE
                     KLRVQLRQYADLPFPVLIEGESGSGKEIIASSCLHFGTQRRERPFLALNCAAISPNLV
                     EATLFGYARGAFTGAVMVKSGYFEDAADGTLFLDEIGELALDLQAKLLRVLENGEYQR
                     LGETRKRVSQARIIAATNRDLRREVKAGNFRADLYHRLSVCSIVAPPLREMGEDKLLL
                     MEHFRHHYAAQTRQPPFRLAAEAEALWRSYAFPGNVRELRNIVIRLTTRHAGQIVGAA
                     ALEVELDLPDEPPPAA"
     gene            1360..2178
                     /locus_tag="PLL14_03590"
     CDS             1360..2178
                     /locus_tag="PLL14_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03035.1"
                     /translation="MPADNLVEIRDLDFAYEDRPILKGITLEIPRGKVVAIMGGSGCG
                     KTTLLRLIGGQLKPSRGEVLVDGLAVPALGADELYALRRRMGMLFQFGALFTDISVFD
                     NVAYQMREHTDLPEELIRDMVLMKLNAVGLRGAHDLMPAELSGGMARRVALARAIALD
                     PMLIMYDEPFAGLDPISLGVIGQLIRKLNDALGATTIMVTHDIQESLLIVDFIYFMSD
                     GKVVALGTPDEIRASKHPFVHQFVFAEADGPVPFHYPAAPLAQELLGRSTYAQS"
     gene            2165..2971
                     /gene="mlaE"
                     /locus_tag="PLL14_03595"
     CDS             2165..2971
                     /gene="mlaE"
                     /locus_tag="PLL14_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HOG03036.1"
                     /translation="MPSPSQGALVAAISSLGRRVNDALQRLGFATRFFGMLLYWSGQS
                     FRRLSLTIREIYFSGFQSLLIIMVSGLFIGMVLGMQGYETLQRYGSADALGVLVALSL
                     VRELGPVVAALLFASRAGSSITAEIGLMKATEQLTAMDMMAVNPIARVVAPRFWGGVI
                     SMPLLAALFSATGVLGGYLIGVVFIGVDEGAFWSQMQASVDFRYDVWSGIVKSFVFGI
                     AVSLIAVFEGYDAVPTAEGVSSSIKGTVVKSALAILSLDFVLTSFMFREA"
     gene            2971..3441
                     /gene="mlaD"
                     /locus_tag="PLL14_03600"
     CDS             2971..3441
                     /gene="mlaD"
                     /locus_tag="PLL14_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOG03037.1"
                     /translation="MSRKLLDLWVGVFVALGLASLLFLALKVGNLAAANFSETYPLTA
                     KFVNIGGLKVRAPVKSAGVVVGRVADIRFDPETYEAVVVFNVDGRYRFPKDTFALILT
                     AGLLGEQYIGLDPGGDETMLKAGEVIAKTQSAVVLEKLISQFMFNKASEGQSGK"
     gene            3457..4176
                     /locus_tag="PLL14_03605"
     CDS             3457..4176
                     /locus_tag="PLL14_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOG03038.1"
                     /translation="MKHSKRVSCVACAAAVLVASGCATTANNPKDPFEGFNRAMFAVN
                     EGLDLVVKPVAQGYDAAAPLPVKVVVGNFFGNIWDVWTAVNNLLQGKGQQAVSDVGRV
                     LINTTAGIGGAFDVAGEMGLTKHSEDFGQTLGKWGVGDGPYFYWPIIGPRTTRDTFGW
                     VVDTYIDPVWRIDDIGLRNSLVATRYISVRASLLPADKVVEEAAFDKYDYIRNAYLQH
                     RRSEIFDGELPHEAVDEESSQ"
     gene            4228..4842
                     /locus_tag="PLL14_03610"
     CDS             4228..4842
                     /locus_tag="PLL14_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG03039.1"
                     /translation="MKALFSLLFALLTLVAPAVAQELAPDAMVRQVTEDVLTVVRQDK
                     DIQSGNTRKAIDLVETKVLPYFNFQRMTALAVGRDWSKATPEQKKRLSEEFKTLLVRT
                     YSNALTSYKNQTVVYKPSKMQAGDTSVVVKTEVVQPGSKPVQLDYSLEKQGDAWKVYD
                     VVVAGVSLVTNYRDTFKQEVSNTGLDGLITMLSNRNKQLEAGKK"
     gene            4839..5132
                     /locus_tag="PLL14_03615"
     CDS             4839..5132
                     /locus_tag="PLL14_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG03040.1"
                     /translation="MIEQADGRLRVTVPMIIANARALLEAGRGLLLSGKNTGLTLLDL
                     AAVSEVDSSALSVVFAWLRTAGTGGIALRIANPPANLISLATLYGVSELLPRA"
BASE COUNT          958 a         1593 c         1636 g         1064 t
ORIGIN      
        1 ccgctgcggg tggcggctcg tcgggcaggt cgagttcgac ttccagcgcc gcggcgccga
       61 cgatctgccc ggcatgcctg gtggtcaggc ggatgacgat gttgcgcagt tcgcggacat
      121 tgccgggaaa tgcgtagctg cgccacaggg cttccgcttc ggcggcgagg cggaacggcg
      181 gctgtcgcgt ttgcgccgca taatggtgac gaaagtgctc catcaggagc agcttgtcct
      241 cccccatctc gcgcaacggc ggggcaacga tcgaacacac cgaaaggcgg tggtaaaggt
      301 cggcacgaaa gtttcccgcc ttgacctccc gacgcaggtc gcggttggtg gcggcaatga
      361 tgcgcgcctg actgacccgc ttccgcgttt ccccgagccg ctgatactcg ccattttcca
      421 gcacgcgcag cagcttggcc tgcaggtcga gcgccagttc gccgatctcg tcgaggaaca
      481 gggtgccatc ggcggcatcc tcgaaatatc cggacttcac cattacggca ccggtaaacg
      541 cacctctggc gtagccgaac agggtggcct cgaccaggtt cggcgatatc gcggcacaat
      601 tgagcgccag gaacggccgt tccctgcgct gggtaccaaa atgcaggcag ctggaggcga
      661 tgatctcctt gccactgccc gactcgcctt cgatcaacac cgggaatggc aaatcggcat
      721 actgtcgaag ttgcacccgc agtttctcaa tggccgggct gctgccgatc agcggcgatg
      781 tgccgtccgc ctggtgctgc ctttcgtcga gcgcgcgaaa cgccagcgct ctcttgagga
      841 cttgctgcag atcggccgga ttgcacggct tggcgacgaa atccgcggcg ccgagcgcgc
      901 gcgcatggcg cgcgttgccc tcgtcgcttt gcccggagag gacgacgatg cggatctccg
      961 gggcggcctt gagcaggtcg gaaatcagcg ccagcccctc gtcgggacga tgcgggactg
     1021 gcggcaggcc gaggtcgacc agggccagtg atggcgctct gcgcaactcg cgcagcagtt
     1081 gcatgcagtg cggacgcgag tgactggtaa ccacttcaaa gagtggacgc atcgaaaaac
     1141 tcaaggtatc acagatcagt gcgtcgtcat cgacgatgag caacagcggt ctttctggca
     1201 aggaggtttc cggtcgagtg ggcatcaagc gctggcgccg cctaaggaat gagcgtgttc
     1261 ggcattattc cacaattgta ttaacgtcaa gtaattcaca gccggccccg gggtgctcag
     1321 gtataattcg cgctcatttt cttgccccgg gatgctgctg tgcctgccga taatctggtc
     1381 gagatcaggg atcttgattt tgcctacgag gatcgcccga tcctcaaggg catcacgctg
     1441 gagatcccgc gcggaaaagt ggtggccatc atgggcggct cgggctgcgg gaaaaccacc
     1501 ctgttgcgcc tgatcggcgg acaactcaaa ccgagccgcg gcgaggttct ggtggacgga
     1561 ctggcggtac ccgccttggg cgccgacgag ttgtacgccc tgcgccggcg catgggcatg
     1621 ctcttccagt tcggcgcgct gtttaccgac atttcggttt ttgacaacgt ggcctaccag
     1681 atgcgggagc acaccgatct gccggaagag ctgattcgcg acatggtgct catgaaactc
     1741 aatgccgtcg gactgcgcgg tgcgcatgac ctgatgccgg ccgagctttc gggcggcatg
     1801 gccagacggg tggcgctggc gcgagcgatt gccctggatc cgatgttgat catgtacgat
     1861 gagccgtttg ccggtctcga tccgatttct ctcggcgtta tcggtcaatt gatccgcaag
     1921 ctcaatgatg cgctgggcgc gacgacgatc atggtcaccc acgacatcca ggaatccttg
     1981 ctgatcgtcg atttcatcta cttcatgtcc gacggaaagg ttgtcgcact cggaacgccc
     2041 gatgaaatcc gggcttcgaa acatcccttc gtgcatcagt tcgtcttcgc cgaagcggat
     2101 ggaccggtcc ccttccacta tccagcggcc ccgctcgctc aggaactgct cggaagaagc
     2161 acgtatgccc agtcctagcc agggagcctt ggtggcggcg atcagcagcc tcggacggcg
     2221 ggtcaacgac gccctgcaac gactggggtt tgcgacgcgc ttctttggca tgctgctgta
     2281 ctggtccggg cagtcgttcc ggcgtctgtc gctgaccatt cgcgagatct atttctcggg
     2341 tttccagtcg ctgttgatca tcatggtttc cggcctgttc atcggcatgg tgctcggcat
     2401 gcagggctac gagacgctgc agcgctatgg ctccgccgac gcactcggcg ttctggtcgc
     2461 gctgtcgctg gtgcgcgagc ttggcccggt ggtcgctgcc ctgcttttcg cctcgcgtgc
     2521 aggctcgtcg atcaccgccg aaatcggcct gatgaaggcc accgagcagc tcaccgcaat
     2581 ggacatgatg gcggtcaatc cgattgcacg cgtcgtggct ccgcgcttct ggggtggagt
     2641 gatctcgatg cccctgctgg cagcgctgtt ctcggccacc ggcgttctcg gcgggtacct
     2701 gatcggcgtc gtcttcatcg gcgtcgacga aggtgccttc tggtcgcaga tgcaggcttc
     2761 ggttgatttt cgttacgatg tctggagtgg catcgtcaag agctttgtct tcggtatcgc
     2821 cgtctccctg attgccgtct tcgagggcta cgacgccgtg ccaaccgccg aaggcgtgtc
     2881 gagttcgatc aagggcacgg tcgtcaaatc ggccctggca atcctgtcgc tcgactttgt
     2941 gttgacctcg ttcatgttcc gggaggcttg atgagtcgca agttgctcga cctgtgggtc
     3001 ggtgtttttg ttgccctggg cttggcttca ttgctgttcc tggcgctgaa ggtcggcaat
     3061 ctggcggcag ccaatttttc cgagacgtac ccgctgacgg ccaagtttgt taacatcggc
     3121 ggcttgaagg ttcgcgcgcc ggtgaaaagc gccggcgtcg tcgtcggacg ggtcgccgat
     3181 attcgtttcg acccggaaac ttatgaggcg gtagtggtct tcaatgttga cggccgttat
     3241 cgcttcccca aggatacctt cgccctgatt ctcaccgccg gcctgctcgg cgagcagtac
     3301 atcggtctcg atccgggtgg tgacgagacg atgctcaagg ctggcgaagt gatcgccaag
     3361 acgcaatcgg cggtggtgct ggaaaagctg atcagtcagt tcatgttcaa caaggcgtcc
     3421 gaaggacaga gcggcaaata gaatgaaagg aaatccgtga aacattcgaa acgcgtgtcc
     3481 tgcgtggcct gtgccgctgc ggttctcgtc gccagtggct gcgccaccac cgccaacaac
     3541 cccaaagacc ctttcgaggg tttcaaccgg gccatgtttg cggtcaacga aggccttgat
     3601 ctggtcgtca aaccggtcgc gcagggctat gacgccgccg caccgctgcc ggtcaaagtg
     3661 gtcgtcggca actttttcgg caacatctgg gacgtctgga cggcggtaaa caatctgctg
     3721 cagggcaagg gccagcaagc cgtttccgat gtcggccggg tgttgatcaa caccaccgca
     3781 ggcattggcg gcgccttcga cgtcgccggg gaaatgggcc tgaccaagca ttccgaggac
     3841 tttgggcaga ccctgggcaa atggggagtc ggcgacggtc cctacttcta ctggccgatc
     3901 atcgggccac gtacgacacg cgatacgttc ggctgggtgg tcgacacgta tattgacccg
     3961 gtgtggcgaa tcgacgacat cgggctgcgc aacagcctgg tcgctacccg ctatatcagc
     4021 gtgcgcgcga gcctcctgcc cgccgacaag gtggtcgagg aagccgcttt cgacaaatac
     4081 gactacatca ggaacgccta cctgcagcat cgccgcagcg agatttttga cggcgagctg
     4141 ccgcacgaag ccgttgacga agaatcctca cagtaggcgg atgcacctcc cgccgggttc
     4201 acgaccccac tttgccagga agcgcccatg aaagctttat tttccttgct gtttgccctg
     4261 ttgaccctgg ttgcccctgc cgtcgcgcag gagctcgcac ccgacgccat ggtccggcag
     4321 gtcaccgagg atgtgctgac cgtcgttcgc caggacaagg acatccagag cggcaatacc
     4381 cgcaaggcca tcgatctggt cgagaccaag gtgctcccgt atttcaattt ccagcgcatg
     4441 accgcactcg ctgtgggacg cgactggagc aaggcgacgc ctgagcagaa gaaacgcctc
     4501 tccgaagagt tcaagacgct gctggtgcgc acctactcga acgcgttgac ttcctacaag
     4561 aaccagacgg tcgtctacaa acccagcaag atgcaggccg gcgataccag cgtggtggtc
     4621 aagaccgaag tcgtgcaacc cggcagcaag ccggtgcaac tggattactc gctcgaaaag
     4681 cagggggatg cctggaaggt ctacgatgtc gtcgtcgccg gcgtcagcct ggtcaccaac
     4741 tatcgcgaca ccttcaagca ggaagtaagc aacacgggtc tcgacggcct gatcacgatg
     4801 ctcagcaatc gcaacaagca actcgaagcc ggcaagaaat gatcgagcag gcggacggca
     4861 ggctgcgcgt tacggtaccg atgatcattg ccaatgcacg cgccctgctt gaggctgggc
     4921 gcggcctgtt gctgtccggc aagaatacgg gcctgacgct gcttgatctc gcggcggtct
     4981 ccgaagtcga ctcctcagcg ttgagcgtcg tctttgcctg gttgcgcacg gccggcaccg
     5041 ggggcatcgc cctgcgcatt gccaatccgc cggcaaacct gatcagcctg gcaacgcttt
     5101 acggcgtgtc cgaactgctc cctcgcgcct gaagggacac tcgtcccgtc accaggcagg
     5161 atcccatgcg catcgccgtt gccatcgatc gcgtcgaaaa gcgtttcggc tccctgctgg
     5221 cgctggccgg cgtttccctg cagattgccg a
//
LOCUS       DAOSIT010000098         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14257_length_5195_cov_10.709922, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000098 DAOSIT010000000
VERSION     DAOSIT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14257_length_5195_cov_10.709922"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLL14_03620"
     CDS             complement(<1..449)
                     /locus_tag="PLL14_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOG03041.1"
                     /translation="MGKKLKELAGSVTENQLASTVKESAQQIWLAGLGAFAKAQEEGG
                     KVFDALVKEGENIQSRTRKITDERIAQVAGKAAGTWDRLEQVFEDRVARALGSLGVPS
                     KQDIDQLSKRVVELSAAVQALTEGKASVKAEATPEEQDESPAAETPA"
     gene            631..>2546
                     /locus_tag="PLL14_03625"
     CDS             631..>2546
                     /locus_tag="PLL14_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG03042.1"
                     /translation="MNYFVTGATGFIGKRLVARLLGRPEATVYFLTRAIELPRLQELY
                     EYWGCDDSRVIPIVGDLTQVDLGVSRTDIHKLHGKIEHFFHLAAIYDLKASAEDQQRA
                     NVDGTRNTVRLAEEIAARHFHLVSSIAAAGLFEGLFREDMFDEAENLENPYFRTKHDS
                     EGIVRKECRIPWRIFRPAIVVGDSRTGEMDKVDGPYYFFKLIQKMRKMFPSWMPTIGI
                     EGGRINVVPVDFVVAAMDHIAHLANEDGQCFHLTDPTPMRVGDLLNTFARAAHAPEMA
                     MRINAALLGFIPRTVRKAMFALTPVRRIRTAVMKDLGLPDDILDFVNYPTRFDCRETQ
                     RALKGSGIAVPPLDSYAWRLWDYWERHLDPDLFVDRTLRGQVEGKVVLVTGGSSGIGK
                     ATIRKLAEAGAVAVTVARDAQALDEVRREFEVAGLHLITHAVDIADPQQCAAFVKFMN
                     EEHGGVDILVNNAGRSIRRGIENSFDRFHDFERTMELNYFGALRLTMGFLPGMIAKRK
                     GHVINISSIGVLTNAPRFSAYVASKAALDAWARCAASEFADRGIEFTTINMPLVRTPM
                     IAPTKLYDQVPTLSPEDAADLVVEAVIHKPVRIATRLGIFGALLHSLMPKVAQITMNT
                     SFRMFPDSGAAARKP"
     assembly_gap    2547..2646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2903..3322
                     /locus_tag="PLL14_03630"
     CDS             2903..3322
                     /locus_tag="PLL14_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HOG03043.1"
                     /translation="MTTPTQNQAPVHNARQLLKELQETFPVFRDSLPLAIGIDKQLQG
                     RLPNVGRKDLRIALGMHTHSLRYLKVMAQATVRVDLDGQPAGEILATHRSHASDLIKE
                     RTRKQAEQRKAQREAEALARQRTEKLDQLVEKFGRSR"
     gene            3435..3995
                     /locus_tag="PLL14_03635"
     CDS             3435..3995
                     /locus_tag="PLL14_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03044.1"
                     /translation="MDHLLKDCAPLELYAWLDDDAEWPGWDILVGNPFAHPLPGDQFT
                     TVQNYFAEYHLEYFRQRLYPHFPSRLHALLLFATRVDAGTFRAKHPARTFGKSLVRAR
                     SKGSYVCSFHDASWLDYLRLPHSLSLSTLDEIANHYWKGALVEEVGLTFLNERWREPP
                     VIEALFQGILEPVTAHPQSGLLPGFT"
     gene            4165..4434
                     /gene="rpsO"
                     /locus_tag="PLL14_03640"
     CDS             4165..4434
                     /gene="rpsO"
                     /locus_tag="PLL14_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG03045.1"
                     /translation="MAINVAQKAQIMSDYQRATGDTGSSEVQVALLTARINDLTGHFK
                     EHKKDNHSRRGLLRMVSRRRKLLDYLKRTNVDSYRSLIQRLGLRK"
     gene            4621..>5195
                     /locus_tag="PLL14_03645"
     CDS             4621..>5195
                     /locus_tag="PLL14_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG03046.1"
                     /translation="MFNIVKKTFAYGAHQVTLETGEIARQAGGAVMVSMNDTVVLVSV
                     VGNKTAKPGQDFFPLTVDYIEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPLRPL
                     FPEGFYHEVQVVAMVMSLNPEIDPDIPAMIGASAALAISGIPFDGPIGAARVGYLDGQ
                     YILNPTATQLSSSLLNLVVAGTQSAVLMVES"
BASE COUNT         1020 a         1657 c         1455 g          963 t
ORIGIN      
        1 ttggcaggag tctctgcagc aggtgactcg tcctgctctt cgggcgtcgc ctcagccttg
       61 acactcgcct ttccttcggt cagcgcctgc accgccgcgc tcaactcgac gacgcgcttg
      121 gaaagttggt cgatatcctg cttgctgggc acgccgaggc tacccagagc acgcgccacg
      181 cggtcctcga acacctgctc aaggcgatcc caagtgcctg ccgccttgcc ggcaacctgt
      241 gcaattctct cgtcggtgat cttgcgggtt cgcgactgga tgttctcgcc ttccttgacc
      301 agcgcatcga acaccttgcc gccttcttcc tgggctttgg cgaaggcgcc aagcccagcc
      361 agccagatct gttgcgccga ttccttgact gtcgacgcca gttggttctc ggtcacgctg
      421 ccggccagtt ctttgagttt cttgcccatt tatatctcct taaacgtagt agtcaaaagg
      481 ccagcaagtt ttttgcttgc cctggaaagc cagtgtagtg acaatatcta gaggacgcac
      541 actaaagagt cagggaatgg cctctgggag gctgcagtcc agcacgcaag gaacacagga
      601 gaggacaatg aatttcatca ggaggcagca atgaattact tcgttaccgg cgccactggt
      661 ttcatcggca agcgacttgt cgcaagattg ctgggccgcc ccgaggctac cgtctatttc
      721 ttgactcgcg ccattgagct gccgaggctg caggaattgt acgagtattg gggttgtgac
      781 gatagccgcg tcattccgat cgtcggcgac ctgacgcaag tagacctggg cgtctcgcgg
      841 accgatatcc acaagctgca tggcaagatc gaacactttt tccatctcgc ggcgatctat
      901 gacctcaagg ccagcgccga agatcaacag cgagccaacg tggacggcac ccgcaacacc
      961 gtccgtctgg ccgaggaaat tgctgccaga cacttccacc tggtcagctc gattgccgcc
     1021 gccgggcttt tcgaagggct gtttcgtgaa gacatgttcg acgaagcgga gaacctcgag
     1081 aatccctact tccgcaccaa gcacgactcc gaaggcatcg tccgcaagga gtgccggatc
     1141 ccgtggcgca tcttccgtcc ggcaatcgtc gtcggcgact cgcgcaccgg cgaaatggac
     1201 aaggtcgacg ggccctacta cttcttcaag ctgatccaga agatgcgcaa gatgttcccc
     1261 tcctggatgc cgaccatcgg catcgaaggc gggcgcatca atgtcgtccc ggtagacttc
     1321 gtggtcgcgg cgatggatca cattgcccac ctcgccaatg aagatggtca gtgcttccac
     1381 ctcaccgacc cgacgccgat gcgcgtcggt gatctgctca acacgtttgc acgcgccgcg
     1441 cacgcaccgg aaatggcgat gcgcatcaat gccgccctgc tcggtttcat cccgcgcacg
     1501 gtgcgcaagg caatgttcgc cctgaccccg gtgcgccgca ttcgtaccgc ggtcatgaag
     1561 gatctcggtc tgcccgacga catcctcgat ttcgtcaact acccgacccg cttcgactgc
     1621 cgcgagacgc agcgcgcgct caagggcagt ggcatcgccg tgcccccgct cgatagctac
     1681 gcctggcgct tgtgggatta ctgggaacgc cacctcgacc cggatctttt cgtcgatcgc
     1741 acgctgcgcg gccaggtcga aggcaaggtc gtgctggtca ccggcggctc ttccggcatc
     1801 ggcaaagcca ccattcgcaa actcgcggaa gccggcgccg tggcggtcac ggtcgcccgc
     1861 gacgcgcagg cgctcgacga agtccggcgc gagttcgaag tcgccgggct gcacctgatc
     1921 acgcatgcgg tggacatcgc cgatccgcag caatgcgccg cctttgtcaa attcatgaac
     1981 gaggagcacg gcggcgtcga tatcctggtc aataacgccg gccgctcgat tcgccgcggt
     2041 atcgagaact ccttcgatcg tttccacgat ttcgagcgga cgatggaact caattacttc
     2101 ggcgcgttgc gtctgaccat gggctttctg ccgggaatga tcgccaagcg caagggacac
     2161 gtgatcaaca tctcgtcgat cggcgtcctc accaacgccc cgcgcttctc ggcctatgtg
     2221 gcctcaaagg cagcgctgga cgcctgggcg cgctgcgcgg catccgagtt tgccgatcgc
     2281 ggaatcgaat tcaccaccat caacatgccc ctggtcagga caccgatgat cgccccgacc
     2341 aagctctacg atcaggtccc aacgctttcc ccggaagacg ccgccgatct ggtcgtcgaa
     2401 gcggtcatcc acaaaccggt gcgcattgcc acacgcctgg gaattttcgg tgccctgctg
     2461 cactccctga tgccgaaggt cgcacagatc acgatgaaca cctccttccg gatgttcccc
     2521 gattccgggg cagcggcgcg caagccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnncgat gaacacctcc ttccggatgt tccccgattc cggggcagcg gcgcgcaagc
     2701 ctgaggaacc ggaatcgcaa acggcggacc agatcgcctt ctcacaggtc atgcgcggga
     2761 ttcatttctg agtgagttgc ccgcagcaag caaccgtctc gcccgctgcg acgagaaacc
     2821 agggggcccc gcatcggacg ccaaggcaag cgcctgctgt cctgagcgag gccccgctca
     2881 cctccctgcc gcatcatcac ccatgacgac tcccactcaa aaccaagcac cggttcacaa
     2941 cgcacggcaa ctcctcaagg aactgcagga gacattcccg gtctttcgtg acagcctgcc
     3001 gctcgccatc ggcatcgaca agcaactgca agggcgactg cccaatgttg gccgcaagga
     3061 cttgcgcatc gctctgggaa tgcacacgca ctcactccgg tacctgaaag tgatggcgca
     3121 ggcaacggtc cgggtggatc ttgacgggca accggcaggc gaaatcctgg ccacgcaccg
     3181 tagccacgcc agcgacctga tcaaggagcg aacccgcaaa caggcagaac agcgcaaagc
     3241 acagcgcgag gctgaggcgc tggctcgcca gcgtaccgag aagctggacc agttggtgga
     3301 gaaattcggc cgcagccgct gagttatcgg caatgcgtac agcgccgtgc tgaatccagc
     3361 gcttccgccg accctgcgga ccaacctacg agacaaaagg cgcaggcgga ttgcggtctg
     3421 tcgagcaaga aatcatggac cacctactca aggactgcgc accgctcgaa ttgtatgcct
     3481 ggctggacga cgacgcagaa tggcccggat gggacatcct cgtcggcaat cccttcgccc
     3541 acccgctgcc cggagaccag ttcacgaccg ttcagaacta cttcgccgag taccatctgg
     3601 aatacttccg gcagcgactc tacccgcatt tcccaagccg gctgcatgct ctcctgctct
     3661 tcgccacacg cgtcgatgcc gggacttttc gcgccaagca tcccgcccgc accttcggca
     3721 agtcactggt gcgagcgcgc agcaaaggca gctacgtctg ctcgttccac gatgcgagct
     3781 ggctggacta cctgcgatta ccgcacagcc tgagcctgtc gactctcgac gaaatcgcca
     3841 atcattactg gaagggcgcg ctcgtcgagg aagttgggct cacctttctg aatgagcgct
     3901 ggcgagaacc gccggtcatc gaagcgcttt ttcaaggtat ccttgagccg gtcacggcac
     3961 acccgcagag cggcttgctg cccggcttca cgtgacggca ccgggaagtc gcccggccac
     4021 aacgatcgcg gtactgcttg ccgcccgccg gctcaacagt tagaatcgcc ggctcgcttc
     4081 gccaagcgaa taagcacctt cattcatctg gcgttggctc tatcaaaccg gggctgcgcc
     4141 gttctttagg aaagagagtt tgacatggcg ataaatgttg cgcaaaaagc gcagatcatg
     4201 agcgactacc agcgtgccac aggcgacacc ggatcgtccg aagtccaggt ggccctgttg
     4261 accgctcgca tcaatgacct gaccggccac ttcaaggaac acaagaagga taaccactcg
     4321 aggcgcggac ttttgcgcat ggtcagccgc cgtcgcaagc tcctcgacta cctgaaacgt
     4381 accaacgttg actcctaccg cagcctgatt cagcgtctcg gcctgcgcaa gtaagcctgt
     4441 tcgccgatcc gcgtcgcgat cggaggtgca ggccattcga aaagccgtca gcggccagcc
     4501 ctcaggtggg ctgcgccgct gtttttgctg ctgcggcttt tgttggcaaa tgggcgcgtc
     4561 ggttgaatgg tctgccggag tcgtgggcag gcacgcgcca gattattgag aaagggaaca
     4621 atgtttaata tcgtcaagaa aaccttcgcc tatggtgccc atcaggtcac cctggaaacc
     4681 ggcgagatcg cccgccaggc gggtggggcc gtaatggtct cgatgaacga cacggtcgtg
     4741 ctggtcagcg tcgtcggcaa caagacagcc aaaccgggcc aggatttttt ccccctaacg
     4801 gtggattaca tcgagaaaac ctatgccgcg ggaaggatcc ccggggggtt cttcaaacgc
     4861 gaggggcgcc cctcggaaaa ggaaacgctg acctcccggc tgatcgaccg cccgctgcgc
     4921 ccgctctttc ccgaaggctt ctaccacgag gtacaggtcg tggcgatggt gatgtcgctc
     4981 aacccggaaa tcgatcccga catcccggcg atgatcggtg cctcggcagc tctggcgatc
     5041 tcgggcattc ccttcgatgg gccgatcggt gccgcacgcg tcggctacct cgacggtcag
     5101 tacatcctca atccgaccgc cacacaactg tcgtcgagtc tgctgaatct ggtcgtcgcc
     5161 ggtacgcagt cggcggtcct gatggtcgag tcgga
//
LOCUS       DAOSIT010000099         5168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14363_length_5168_cov_9.826716, whole genome shotgun sequence.
ACCESSION   DAOSIT010000099 DAOSIT010000000
VERSION     DAOSIT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14363_length_5168_cov_9.826716"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..927
                     /locus_tag="PLL14_03650"
     CDS             7..927
                     /locus_tag="PLL14_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOG03047.1"
                     /translation="MKLALGPLLYFWPKNEVFEFYAEMAQNPAIDIIYLGEVVCSRRQ
                     QIRVADWLGLARDLSDAGKEVVVSAQALLESEGDLKALRRLADELTEIPGCRLEANDL
                     GAVNIAAGRPFVAGPHLNIYNEATLATFARYGMCRWLPPLEGDRRLIETLHRSRPQGV
                     DTEVFVFGKLPLAFSARCFTARHYNLNKDDCQFKCLDHPEGLTLRTREGQPFLTINGI
                     QTMSAQTYNLLPQMPELLALGIEVVRLSPQPKSMPAIVAAFDAARHGQRVDVDSSGWA
                     SDGLVDGYWFADAGIVCHRSGAAAHLQGTL"
     gene            927..1373
                     /locus_tag="PLL14_03655"
     CDS             927..1373
                     /locus_tag="PLL14_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOG03048.1"
                     /translation="MSFSIPRFKLPGVLSTIGARLPQWPHSLALASALNVAARIGLLP
                     DDSLTQLEGRSFLVEVIDAGGQACFCYRDGLFRPLFTAPASPDLSFRANLSAFLQLLA
                     RQEDPDTLFFNRELSIVGDTELGLVIKNMLDAIEWPQLPTLPIFRH"
     gene            1398..2351
                     /locus_tag="PLL14_03660"
     CDS             1398..2351
                     /locus_tag="PLL14_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG03049.1"
                     /translation="MRPELPASRVLVEGIACALGAGLLWGLVFVVPLMLPDYPPAILS
                     CGRYLGFGVIALLLAGTDLPRLQRLQRQDWRMACELALVGNLLYYLFLAAAIQLADAP
                     LPTMLIGTLPIVIAIAANFGSEALPWRRLLPSLLVIAVGIALVNSHEMAQLGTRRSPH
                     DYLLGALLAVAAVLAWTWYPLRNSHWLRQRPTLASGTWAVAQGLATLPLAAVGMAAAA
                     FLFQDPGGFDYPLGPQPLRYVGLMLTLGLCASWLGTLLWNRASRLLPTALTGQLIVFE
                     TLSAFAYAFIWRGALPGGMALLGIALLVSGVILGVRAFRAG"
     gene            complement(2308..3123)
                     /locus_tag="PLL14_03665"
     CDS             complement(2308..3123)
                     /locus_tag="PLL14_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG03050.1"
                     /translation="MSAPVIVLRAVVCVAGGRAVLEIEDLAIAPGERIAIVGHNGAGK
                     STLLRLLSGFSVATHGRVEVLGRVLGAALPAAELRALRRETGQVLQGLHLVSRLSALD
                     NVLIGCLGRVGGWRSWARCYPEQERAAAAAALQAVGLLARAQSRADTLSGGERQKVAI
                     ARLLVQQPRLILADEPTAALDPAAAQEVCQLLARAGRTATLISVLHNPAQLPLLAERV
                     IGLREGKIAFDLPLAAVGEQALGDLYRPAARMRASGCPAISRPGTRVLRGSPR"
     gene            complement(3120..4016)
                     /gene="phnE"
                     /locus_tag="PLL14_03670"
     CDS             complement(3120..4016)
                     /gene="phnE"
                     /locus_tag="PLL14_03670"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOG03051.1"
                     /translation="MHPALAAAERRAGPLRGRLGFLALALAYGLLVAACLGSLGREGE
                     LSLGRTPLANLLKTATEFARPSFVDAWWGNPQLEYVSDDGTVLRVEDRREVERHYLAG
                     LAEASWTTIRIATLGSLLGALLALPLAVLVARNLAAPRALAWLAKGVLDVARSIHTLV
                     FGLVLVGIVGLGPTAGILAIGLHSLGSYGKLFAEAIETLDMAAVDAVRSVGAGPAQVF
                     FNAIWPAVLPQFVSSHLYIWEFNIRDSTILGIIGAGGLGLLISEATALFQWGRLATVL
                     IVVIALVASFDAMSRCIRKALL"
     gene            complement(4019..4885)
                     /locus_tag="PLL14_03675"
     CDS             complement(4019..4885)
                     /locus_tag="PLL14_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG03052.1"
                     /translation="MKRSFHLFVLLVTTLLATCGFAAEPPLKFGVGLFQPDRDKNDST
                     YRPLANYLSAKLDRPVELRTVDSWEGLAKSLANGETDIALLGPWGYVLANHEAGAQVL
                     ATILYDGKPEYFAIMVTHPQSGVSSVADLKGRTFAFGDKGSTSGYLIPLHYFMSQGIN
                     PDSYFAKVLHTKHQAIEMQVSQGTLDAGADYNRNRNAMIEQGLLKPEHSRIIWQSAPL
                     PNDAVAVSKALFVDRALVARLQAALLGIGDALKTQPQLLPAHYTGFVIRDNAFYKPIR
                     DAGLATGKLQVR"
BASE COUNT          841 a         1823 c         1664 g          840 t
ORIGIN      
        1 accgcgatga aactcgctct cgggccgctg ctctacttct ggccgaaaaa cgaagtcttc
       61 gagttctatg ccgagatggc acagaacccg gcaatcgaca tcatctacct cggcgaggtg
      121 gtctgctcgc gccgccagca gattcgtgtc gccgactggc tgggactggc gcgcgacctc
      181 agcgacgccg gcaaggaagt ggtcgtttcg gcacaggcgc tgctcgaatc ggaaggcgac
      241 ctcaaggcgc tgcgccgcct ggctgacgaa ctgacggaaa ttcccggctg ccggctcgaa
      301 gccaacgatc tcggcgcggt gaacatcgcc gccggccgcc cctttgtcgc cggcccgcac
      361 ctgaacatct acaacgaagc gacgctggcc acctttgccc gttatggcat gtgtcgctgg
      421 ctgccgccgc tggagggcga ccgccggctg atcgaaaccc tgcaccgcag ccgcccgcaa
      481 ggcgtcgaca ccgaggtctt cgtcttcggc aagctgccgc tggccttttc cgcgcgctgc
      541 ttcaccgcgc ggcactacaa cctcaacaag gacgactgcc agttcaagtg tctcgatcac
      601 cccgagggac tgaccctgcg cacccgcgag gggcagccgt tcctgaccat caacggcatc
      661 cagacgatgt cggcgcagac ctacaacctg ttgccgcaga tgccggagct gctggcgctg
      721 ggcatcgagg tcgtgcgcct gagtccgcaa ccgaagtcca tgccggcgat cgtcgccgcc
      781 ttcgatgccg cccgccatgg ccagcgggtc gacgtcgaca gctccggctg ggccagcgac
      841 ggactggtcg acggctactg gttcgccgat gccggcatcg tttgccaccg gtcgggtgcc
      901 gccgcacacc tgcaaggaac cctgtgatga gcttttcgat accccgtttc aaactgcccg
      961 gcgtactgtc cacgatcggc gcccgcctgc cgcaatggcc gcattcgctg gcgctcgcca
     1021 gcgcgctcaa tgtcgcggcg aggattggcc tgctccccga cgacagcctg acgcagctcg
     1081 aagggcgaag cttcctcgtc gaggtgatcg acgccggcgg gcaggcgtgc ttctgctacc
     1141 gcgacggcct cttccggccg ctgttcaccg cccccgcgag ccccgatctg tcgttccgcg
     1201 ccaacctctc ggcctttctg caactgctcg cccgccagga agatccggac acgctgttct
     1261 tcaaccgcga actgtcgatc gtcggcgaca ccgaactcgg cctggtgatc aagaacatgc
     1321 tcgacgcgat cgagtggccg cagcttccga cgctgccgat ctttcgtcac tgacctccgc
     1381 aagagccggc gcgaaccatg cggccagagc tgccggccag ccgcgtcctg gtcgagggca
     1441 tcgcctgtgc gctcggtgcc ggcctgctct ggggactggt cttcgtcgtg ccgctgatgt
     1501 tgcccgacta tccgccggcg atcctctcct gcgggcgcta tctcggtttc ggggtcatcg
     1561 cgctgctcct cgccggcacc gacctgccgc gcctgcaacg cctgcagcgc caggactggc
     1621 ggatggcttg cgagctggcg ctggtcggca acctgctcta ctacctgttt ctggccgcgg
     1681 ccatccagtt ggccgacgcc ccgctgccga cgatgctgat cggtaccctg ccgatcgtca
     1741 tcgccatcgc cgccaacttc ggcagcgaag ccctgccgtg gcgtcgcctg ctgccttcgc
     1801 tgctggtcat cgcggtcggc atcgcgctgg tgaacagcca tgaaatggcg cagctcggca
     1861 cgcggcgcag cccccacgac tacctgctcg gggcgctgct ggcggtcgcc gcggtactcg
     1921 cctggacctg gtacccgctg cgcaactcgc actggctgcg gcagcgaccc accctcgctt
     1981 ccggcacctg ggccgtcgca caggggctgg cgacgctgcc actggccgcc gtcggcatgg
     2041 ccgccgcggc tttcctgttc caggaccccg gcggcttcga ctacccgctc ggcccgcagc
     2101 cgctacgtta tgtcgggctg atgctgaccc tgggactgtg tgcctcgtgg ctgggcacgc
     2161 tgctctggaa ccgcgccagc aggctgctgc cgacggcgct gaccgggcaa ctgatcgttt
     2221 tcgagaccct ctcggccttt gcctacgcct tcatctggcg cggcgcgctg cctggcggca
     2281 tggccctgct cggcatcgcc ctgctggtca gcggggtgat cctcggagta cgcgcgttcc
     2341 gggccggctg atcgccgggc atccagacgc ccgcatgcgc gcggcgggcc gatagagatc
     2401 accgagcgcc tgctcgccga ccgccgccag cggcagatcg aaggcgattt tcccctccct
     2461 gaggccgatc acccgctcgg caagcaaagg cagctgcgcg gggttgtgca acacgctgat
     2521 cagcgtcgcc gtgcggcccg ccctggccag gagctggcag acctcctgag cggccgcggg
     2581 gtcgagggcc gccgtcggct cgtcggcgag aatcagccgc ggctgctgca ccagcaggcg
     2641 ggctatcgcc accttttgtc gctcgccgcc ggacagcgta tcggcgcgcg attgcgcccg
     2701 cgccagcagc cccaccgcct gcagcgcggc cgctgcggcg gcgcgttcct gctccgggta
     2761 gcagcgcgcc caactgcgcc agcctccgac gcgaccaagg cagccgatca gcacgttgtc
     2821 cagcgccgac agtcgcgaca ccagatgcag gccctgcagc acctgcccgg tttcccgacg
     2881 caaggcgcgc agttcggccg cgggcagcgc ggctccgaga acacgcccca ggacctcgac
     2941 gcgaccgtgc gtcgcgacgc tgaagccgct cagcagacgc agcaaggtcg acttgccggc
     3001 gccgttgtgg ccgacgatgg cgatgcgctc gccgggcgcg atggccaggt cttcgatctc
     3061 cagcacggcg cggccgccgg ccacgcagac caccgctcgc agcacaatca ccggcgcgct
     3121 cacaataacg ccttgcggat gcaccgactc atggcgtcga aggaagccac cagggcgatc
     3181 accacgatca gcacggtcgc caaccgcccc cattgaaaga gcgcggtcgc ttccgagatc
     3241 agcagaccca ggccgccggc gccgatgatc ccgagaatcg tcgagtcgcg aatgttgaat
     3301 tcccagatgt acaggtggct ggaaacgaac tggggtagaa ccgccggcca gatggcgttg
     3361 aagaagacct gcgccgggcc ggcgccgacg ctgcgcaccg catcgaccgc cgccatgtcg
     3421 agggtctcga tggcctcggc gaagagcttg ccgtagctgc ccagcgaatg cagaccaatg
     3481 gccaggatgc cggcggtggg acccagcccg acgatgccca ccagcaccag cccgaatacc
     3541 agggtatgga tcgaacgcgc cacatcgagc acgcctttcg ccagccaggc cagcgcgcgc
     3601 ggcgccgcga ggttgcgcgc caccagcacc gccagcggca gtgccagcaa ggcgccaagc
     3661 aaggagccca gggtggcgat gcgaatcgtc gtccagctcg cctcggcaag tcctgccaga
     3721 tagtggcgct cgacctcgcg gcggtcctcg acgcgcagaa cggtgccgtc atcgctgaca
     3781 tattcgagtt gcgggttgcc ccaccaggca tcgacgaagc tcgggcgggc gaactcggtg
     3841 gccgtcttca gcaggttggc gagtggggtt cgccccagcg acaactcgcc ttcgcggccc
     3901 agcgatccca gacacgccgc gaccagcagg ccataggcca gcgccagtgc gaggaaacca
     3961 agacggcccc gcagcggacc cgcgcgccgt tcggcagcgg ccagggccgg gtgcatgttc
     4021 agcgcacctg cagcttgccg gtcgccaggc cggcgtcgcg gatcggcttg tagaatgcgt
     4081 tgtcgcggat gacgaagccc gtgtagtgcg ccggcaacag ctgcggttgg gttttcaggg
     4141 cgtcgccgat gcccagcaga gcagcctgca gacgggccac cagcgcacga tcaacgaaca
     4201 acgccttgct gaccgcaacg gcgtcgttcg gcaacggcgc cgactgccag atgatcctgg
     4261 agtgctccgg cttgagcagc ccctgctcga tcatcgcgtt gcggttgcgg ttatagtcgg
     4321 cgccagcgtc gagagtgccc tggctgacct gcatttcgat cgcctggtgc ttggtgtgca
     4381 gaaccttggc gaagtagctg tcggggttga taccctgact catgaaataa tgcagcggaa
     4441 tcaggtagcc cgaggtggaa cccttgtcac cgaaggcgaa agtcctgccc ttcaggtcgg
     4501 cgacgctgct cacgcccgac tgcggatgcg tgaccatgat cgcgaaatat tccggcttgc
     4561 cgtcgtagag gatggtcgcc agcacctgcg cgccggcctc gtggttggcg agcacgtaac
     4621 cccagggccc cagcagggcg atgtcggtct caccgttggc cagcgacttc gccagcccct
     4681 cccagctatc gacggtgcgc agttcgaccg ggcgatcaag ctttgccgac aggtaattgg
     4741 ccagcggacg gtaggtgctg tcgttcttgt ccctgtccgg ctggaagaga ccgacgccaa
     4801 atttcagtgg cggctcggcg gcaaacccgc aggtggccaa caaggtcgtc accagcagca
     4861 cgaagaggtg gaacgagcgt ttcatgagtc ctccggaaag acgctaacga tgggttcagg
     4921 gaaactgcag cccgctcgga tgtgcgggcc gttgccaggc agtggtcatg tcgcttggtc
     4981 gcggcgaacg tcgcgatcct tacagcccca cgcacccgac gcggcgctca cgttgccaac
     5041 agcaccccat cgacgtcttt cagcgtcccg acacgcagca ggtcgcggac cagccattgc
     5101 gcgagttgct cattgctgtg attcaggtaa cgggcgttga cgctctggca gaaactcagg
     5161 ctggcgag
//
LOCUS       DAOSIT010000100         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14490_length_5134_cov_10.049616, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000100 DAOSIT010000000
VERSION     DAOSIT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14490_length_5134_cov_10.049616"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLL14_03680"
     CDS             complement(<1..1040)
                     /locus_tag="PLL14_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOG03053.1"
                     /translation="MGFQVSVEPGRHSFQAESDETILEAALRQGLSLPYGCRNGFCGS
                     CRGKVLTGEVEHGAAPIEVLSNADRAAGFALFCCAMARSDLRIESREVRSFEDIPIRT
                     LPARVQRLTRAAPDVMIVELKLAANERLQFLAGQYVDILLKDGRRRAFSLANAPHADT
                     CLQLHIRHAPGGQFTTQVFETMKERDILRLRGPLGTFTLCEESPKPMLLIAGGTGFAP
                     IKAIVEHAIVETNARPMHIYWGGRQRADLYLLQLAEQWAVEHAHIRFTPVLSDATTAD
                     RWIGRRGLVHEAAMADHPDLSEHQAYVCGSPAMVAAARQDFVARCRLPAEEFFADSFE
                     FAADTLNAMVTSP"
     gene            1080..1988
                     /locus_tag="PLL14_03685"
     CDS             1080..1988
                     /locus_tag="PLL14_03685"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOG03054.1"
                     /translation="MQKLLIVGCGDIARRILPRLRGHYRVFALLRDPSQRAFWREHGA
                     SPVLADLDQPDSLQRIAGLADIILYLAPPAAADKYHVTGSGRDARTSALLAALARGWS
                     LPQRIVYISTTGVYGDCDGKLIDETHALRPATARACRRVDAERQLRAFGRRGVVVSIL
                     RAPGIYAADRLPIERLRKGIPALYAPDDVYTNHIHADDLAMLTCAAVRYGRNNRTYNA
                     TDDSQIKMGDYFDLVADGFGLPRAPRLSRREVEERLSPLQFSFMSESRRLRNQRIKQE
                     LRVKLRYARIEDGVAAARMERNNWCS"
     gene            1979..2395
                     /locus_tag="PLL14_03690"
     CDS             1979..2395
                     /locus_tag="PLL14_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOG03055.1"
                     /translation="MLIVKVLHLWMVVSWFAGLFYLPRIFVNLAMVAPDSVAERDRLL
                     LMAGKLYRFMTPIAILAVALGCWLWFGFGFSGGWLHLKTTLVLGLLAYHAYCGRLLRD
                     FRAGRNVRSHVWFRWFNEMPVLVLLAVTYLAVLKPF"
     gene            2415..>3098
                     /locus_tag="PLL14_03695"
     CDS             2415..>3098
                     /locus_tag="PLL14_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOG03056.1"
                     /translation="MVETFFASCPRGLEQLLVDDLSAAGARDLKPISGGVHFRAEWPV
                     CYRANLHSRIATRILWRVAHAPYRTEDDIYRLALETSWPRSFVSEQTIRVDVTAVKSA
                     LKSIDFVTLRIKDAICDRFRRDTGKRPNVDTRQPDVRVHGFLGSEDCTLYLDTSGAPL
                     YQRGFRQKTVDAPLKENLAAGILRLSGWHPGVTLLDPMCGSGTFLIEAAQIALGIAPG
                     AGRSFAFQRL"
     assembly_gap    3099..3198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3199..3384
                     /locus_tag="PLL14_03700"
     CDS             <3199..3384
                     /locus_tag="PLL14_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG03057.1"
                     /translation="LKRRFAGWNCYLLSADTRLPKLMRLTPSRKTPLFNGALECRLYE
                     FRMVAGSNRGTTTTPPV"
     gene            complement(3474..4610)
                     /locus_tag="PLL14_03705"
     CDS             complement(3474..4610)
                     /locus_tag="PLL14_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG03058.1"
                     /translation="METDQALRFMHELLRLMTQKNGSDLFITANFPPAIKIDGKVVPV
                     SNQPLLPQHSAELARSIMNDRQASDFEATKECNFAISPTGIGRFRVSALVQQGKVAIV
                     CRTINLVVPSLDELGLPPVLKDIAMTLRGLVVFVGGTGTGKTTSLAALVDHRNRNSQG
                     HIITIEDPIEFIHEHKKCIVTQREIGVDTDSWEIALKNTLRQAPNVIMMGEIRDRATM
                     DYAIAFAETGHLCLATLHANSTNQAIDRIINFFPEERRHQLLMDLSLNLRAMVSQRLL
                     PKKDGKGRAPAIEIMLNSPLISDLIFKGQVQEIKEVMKRSRELGMQTFDQALFDLYEA
                     GQISYTDAMRNADSLNDLRLQIKLHGKESKDRDLTAGIGHLDII"
     gene            complement(4638..>5134)
                     /locus_tag="PLL14_03710"
     CDS             complement(4638..>5134)
                     /locus_tag="PLL14_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG03059.1"
                     /translation="FQNALRSALREDPDVILVGELRDLETIRLALTGAETGHLVFATL
                     HTSSAAKTIDRVIDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEILIG
                     TPAIRNLIRENKVAQMYSSLQTGQQHGMQTLDQCLVDMVRRNVVSAAEARNKAANKDT
                     FPGL"
BASE COUNT          882 a         1476 c         1594 g         1082 t
ORIGIN      
        1 ggactggtga ccatggcatt cagcgtatcg gcagcaaact cgaaggagtc ggcgaagaac
       61 tcttccgcag gcaaccggca tcgagccacg aaatcctgtc ttgcggctgc gaccattgcc
      121 ggagaaccac agacgtaggc ctgatgttcg gaaaggtcgg gatgatcggc catcgccgcc
      181 tcgtgaacca ggccgcggcg accaatccag cggtccgccg tggtggcgtc ggagagcacc
      241 ggggtgaagc ggatgtgcgc atgttccact gcccactgct cggcaagttg cagcaggtag
      301 agatcggcac gttggcgccc accccaatag atgtgcatcg gtctggcgtt ggtttccacg
      361 atggcatgtt cgacgatcgc cttgatcggc gcaaatccgg tgccgccggc aatcagcaac
      421 atgggtttcg gcgattcctc gcagagcgtg aaagtaccca gtggtccacg caggcggagg
      481 atgtcgcgtt ccttcatggt ttcgaaaacc tgcgtggtga actgtccacc cggagcgtgc
      541 cggatgtgca actgcaggca ggtgtccgca tgcggggcgt tggccagcga aaaagcgcgg
      601 cgacgcccgt ccttgaggag gatgtccaca tactgtcccg cgagaaactg cagccgctcg
      661 ttggccgcca gtttgagttc gacgatcatc acgtcgggag ctgcgcgcgt cagccgctgc
      721 acgcgcgccg gcaaggtgcg gatcgggatg tcttcaaacg agcgaacttc ccggctttcg
      781 atgcgcagat cgcttctggc catggcgcag cagaagaggg cgaagccggc cgcgcgatcg
      841 gcgttgctga gtacctcgat gggtgccgcg ccgtgctcca cttctcccgt cagcaccttg
      901 cctcggcaac tgccgcagaa gccattgcgg cagccgtaag gtaggctcag cccctggcgt
      961 agtgctgctt cgagaatcgt ttcgtcggac tcggcttgaa aactgtgccg gccaggttcg
     1021 acactgacct gaaaacccat tgcctctccg ctgatgtccc aatccggtaa aattgcgcag
     1081 tgcaaaaact tttgatcgtc ggctgcggtg acattgcccg ccgcatcctg ccccgcctgc
     1141 gcggccatta ccgtgttttc gcgctgttgc gcgacccgtc ccagcgcgcc ttctggcgcg
     1201 aacacggggc cagtcccgtg cttgccgacc tcgatcagcc tgacagcctg cagcgcatcg
     1261 ccggtctggc cgatatcatc ctgtatctgg caccgccggc ggccgcggac aagtaccatg
     1321 tcaccggtag cgggcgcgat gcccggacca gtgccctgct cgccgccctg gcccgcggct
     1381 ggagtctacc acagcgaatc gtgtacatca gcaccaccgg tgtctacggc gattgtgacg
     1441 gcaaactgat tgacgagacg catgccttgc ggccagccac ggcccgagcc tgtcgccggg
     1501 ttgacgccga gcgtcagttg cgcgccttcg ggcgccgtgg ggtggtggtc agcattctgc
     1561 gtgcccccgg catttatgcc gccgatcgcc tgccgatcga gcggttgcgc aagggaattc
     1621 ccgccctata tgcgccggac gacgtgtata ccaaccatat tcatgccgac gatctggcga
     1681 tgctgacttg cgccgcagtt cgttacggac gcaacaaccg cacgtacaat gcgacagacg
     1741 attcgcagat caagatgggg gactattttg atctggtggc cgacggtttc ggtttgccga
     1801 gggctccgcg tctgtcacgt cgcgaagtcg aggagcggct ctcgcccctg cagttctcgt
     1861 tcatgagtga atcacgccgt ttgcgcaacc agcggatcaa gcaggaactg cgggtgaaat
     1921 tgcgttatgc gcgtatcgag gatggcgtgg cagcggcacg gatggaaagg aataactggt
     1981 gctcatagtc aaggttctgc atctgtggat ggtggtcagc tggtttgcgg gcctgttcta
     2041 cctgccgcgc attttcgtca acctggcgat ggttgcaccg gacagcgtcg ccgaacgcga
     2101 tcgcttgctg ctgatggctg gcaagctcta tcgtttcatg accccgatcg ctattctggc
     2161 agtcgctctc gggtgctggt tgtggtttgg cttcggcttt tcaggtggct ggctgcacct
     2221 caagacaacc ctggtgttgg ggttgctggc ctaccacgcc tattgtggtc ggctgctgcg
     2281 cgactttcgc gccggccgta acgtgcgtag ccacgtctgg tttcgctggt tcaacgaaat
     2341 gccggtgctg gtcctgctgg cggtgactta ccttgccgtt ctcaagccgt tttagttcac
     2401 ctggccagga gacaattgtg gaaactttct ttgccagctg cccgcgcggc ctggaacaac
     2461 tgctggttga cgatctttcg gcggccggag cgcgcgacct caaaccgatc tccggtggcg
     2521 tgcattttcg tgccgagtgg ccggtttgct atcgggccaa tctgcattcg cgaattgcca
     2581 cgcgcatcct gtggcgggtg gcgcatgcgc cttaccgcac cgaggacgac atctaccgcc
     2641 tggcgctgga gacgtcgtgg ccacgcagtt ttgtttccga gcagaccatt cgggtcgatg
     2701 tcacggccgt aaaaagcgca ctgaaaagca ttgatttcgt gaccctgcgc atcaaggatg
     2761 cgatctgcga ccgtttccgc cgcgacacgg ggaagcgccc gaacgtcgat acgcgccaac
     2821 cagacgtgcg ggtacacggt tttcttggca gcgaggactg tacgctttat ctcgatacct
     2881 cgggtgcccc gctttatcag cgtggttttc gccagaagac cgtggatgcg ccgctgaagg
     2941 aaaacctggc cgcgggtatt ctccgcctca gcggttggca tccgggggtc acgctgctcg
     3001 acccgatgtg cggcagcggt accttcctga tcgaagcggc ccagatcgca ctcggcattg
     3061 cgccaggggc cggacggagt ttcgctttcc aacggctgnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnct gaagcgccgg ttcgcgggct ggaactgcta cctgttgagc
     3241 gccgacacgc gtttgccgaa gttgatgcgc ctgacaccaa gcaggaagac gcccctattc
     3301 aatggcgcgc tcgaatgccg cctctatgaa ttccggatgg tggccggcag caatcgaggt
     3361 acgaccacga cgccgccggt gtaggcgtca gtcggtgtat tggcggcttg tcgggagaac
     3421 cgattgcggc cggaacagca gcggttaccg ggtttcctgc cgccacttgc tcgtcaaatg
     3481 atgtccagat gtccgatgcc agcggtcaga tcccgatctt tcgattcctt gccatgcagc
     3541 ttgatctgca ggcgcaggtc gttgagcgag tcggcgttgc gcatggcatc ggtgtagctg
     3601 atctggccgg cttcgtatag atcgaacagc gcctggtcga aggtctgcat gcccagttcg
     3661 cgcgaccttt tcatgacctc cttgatttcc tgcacctggc ccttgaagat gaggtctgaa
     3721 atcagtggcg agttgagcat gatctcgatc gccggggcac gtcccttgcc gtccttcttc
     3781 ggtagcagtc gctgcgaaac catggcccgc aggttgagcg aaaggtccat cagcaactgg
     3841 tggcggcgtt cctcggggaa gaagttgatg atccggtcga tggcctggtt ggtactgttg
     3901 gcatgcagcg tcgcaaggca gagatgcccg gtttcggcga aggcgatggc gtagtccatg
     3961 gtcgcgcgat cgcggatttc acccatcatg atcacgttcg gtgcctgccg cagcgtgttc
     4021 ttcaatgcga tttcccaact gtcggtatca acgccgattt cgcgttgggt gacgatgcat
     4081 ttcttgtgtt catggatgaa ttcgatcggg tcttcgatgg tgatgatgtg accctgactg
     4141 ttgcggttgc ggtgatcaac gagtgccgcc agagaggtgg tcttgccggt gccggtgccc
     4201 ccgacaaaaa cgaccaggcc gcgcaaggtc atcgcgatgt ctttcagcac cggtggcagg
     4261 ccgagctcgt cgagggacgg tacgaccaga ttgatggtgc ggcagacgat ggcgaccttg
     4321 ccctgttgta ccagggcgct gacacggaag cggccgattc ccgtcggcga gatggcgaag
     4381 ttgcattctt ttgtggcctc gaaatcggag gcttgccgat cgttcatgat tgaacgcgcg
     4441 agttcagcgg agtgctgcgg cagcaggggt tggttggaaa ccggtaccac cttgccgtcg
     4501 atcttgatgg ccggcgggaa gtttgccgtg atgaagaggt ccgaaccatt tttctgcgtc
     4561 atcagtcgca gcagttcgtg catgaatctt agcgcctggt cagtttccac gcatctcctc
     4621 ctgcttgcaa ggctggctca aaggccggga aaagtgtctt tattggccgc cttgttgcga
     4681 gcttcggctg ccgaaacgac attgcgtcgg accatgtcga ccaggcattg gtcgagcgtc
     4741 tgcatgccgt gctgttgccc ggtctgcagg gacgaataca tttgcgccac cttgttttcc
     4801 cgaatgaggt tgcggatcgc cggtgtgccg atcaggatct catgcgccgc gacgcggccc
     4861 tggccgtcct tggtcttgag cagggtctgc gagatcaccg cccgcaggga ttcggaaagc
     4921 atcgagcgaa ccatttcctt ttcggcggcg gggaacacgt cgattacacg gtcaatcgtc
     4981 ttggcagcgc tcgaggtatg cagggtggcg aagaccaggt gcccggtttc cgcaccggtc
     5041 aacgccagac ggatggtctc gaggtcgcgc aactcaccga ccaggatcac gtccggatct
     5101 tcgcgcaagg ccgagcgcag agcgttctgg aaag
//
LOCUS       DAOSIT010000101         5123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14523_length_5123_cov_10.832478, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000101 DAOSIT010000000
VERSION     DAOSIT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14523_length_5123_cov_10.832478"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLL14_03715"
     CDS             <1..826
                     /locus_tag="PLL14_03715"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOG03060.1"
                     /translation="KVPLLRGKSVFNVFFENSTRTRTTFEIAAKRLSADVINLDVTRS
                     STAKGETLLDTVDNLVAMHADMFVVRHAASGAPHLIADHLRRLGRNDVHVVNAGDGSH
                     AHPTQGLLDMYTIRHYKKDFTNLAVAIVGDILHSRVARSDIHALTTLGAAEVRAVGPQ
                     TLLPTAIEKMGVRVSHDLGTGLRDCDVVIMLRLQNERMNGALLPSAREYFRCYGLTPA
                     MLALAKPDAIVMHPGPMNRGVEIDSDVADGPQAVILPQVTFGIAVRMAVMGILAGN"
     gene            830..>2053
                     /locus_tag="PLL14_03720"
     CDS             830..>2053
                     /locus_tag="PLL14_03720"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOG03061.1"
                     /translation="MNRQHLHLRNARLIDPKISLDAHLDLFIADGLVAGIGQPPTGFA
                     AERSIDASGLIACPGLVDLSSRLGGIEPELNAAVAGGVTSLACPPDSKPPLDEPGLVE
                     RLVRRSETLGLARVYPIGALTQQLAGEKLAEMNSLARAGCIAFSQAKRAIVDTQLLLR
                     AMQYAATFGYAVRLRPQDQFLTGDGVAHDGEVASRLGLASIPVSAETVAIATALLLAS
                     QTGVRLHLSRLSSAAGIALIRQARRSNVQVSCDVAVHHLHLSEMDIGYFDSNARFDPP
                     LRSASDRDALRAAVAEGLAAICSDHTPVDADGKQLPFAEALPGATGLELLLPLTLQWA
                     AQEKLPLSLALARITCDPAAILGIEAGSLSVGSPADVCVFDPDESWRVTPEALRSQGK
                     NTPFMGYEVSGRVRMT"
     assembly_gap    2054..2153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2154..2398)
                     /locus_tag="PLL14_03725"
     CDS             complement(<2154..2398)
                     /locus_tag="PLL14_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOG03062.1"
                     /translation="MPDWLREVDGAIILTIHVQPGAKRSEVAGEYGDALKIRLAAPAV
                     DGRANAALLAFIAQRLGLARSAVELKSGLTSRRKSLRV"
     gene            complement(2403..2948)
                     /locus_tag="PLL14_03730"
     CDS             complement(2403..2948)
                     /locus_tag="PLL14_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOG03063.1"
                     /translation="MIIQASLFLIEALTGFLTFALLLRFYMQACRVSFNNQIGTFVVE
                     LTNWLVRPLRKVLPGLFGLDLASLLPAYVLQVLFACAVFVLRGGIAMEAWSLEVLIPL
                     LLWRGVVATLRLSLYLLIGALLAQAVLSWVNPYSPFSQPVSQLTRPVLQPIQRVVPPI
                     GAIDLSPLIAIVLAQLLLIFL"
     gene            complement(2948..3763)
                     /gene="proC"
                     /locus_tag="PLL14_03735"
     CDS             complement(2948..3763)
                     /gene="proC"
                     /locus_tag="PLL14_03735"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOG03064.1"
                     /translation="MKITFLGGGNMANAMIGGLVKTGFSAADIAVIELGAQGRAKLRL
                     VYGVRCYEAPTAAALDCDAVLLSVKPQQLRDACEPLLRHLKQQLIISIAAGLRLADVS
                     RWLGGYGKIVRAMPNTPALIGAGVTGLYALPAVSMAERLGAERILQAVGSTLWIADEA
                     QMDAVTAISGSGPAYVFLFIEALQQAAKELGLTPAQARQLSIDTVLGAAKLASQSDEA
                     ASVLRERVTSKGGTTEAALRTMAERGVKEGFIAGVFAANARGRELGELLGGDA"
     gene            complement(3760..4449)
                     /locus_tag="PLL14_03740"
     CDS             complement(3760..4449)
                     /locus_tag="PLL14_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG03065.1"
                     /translation="MTTLGARLQDVLARIARAAQQHGRDPAKVLLLAVSKTWPSASVA
                     ELAAAGQRAFGESYVQEGVDKISALRSLRLDWHFIGSLQSNKTRIVAESFDWVHSVDR
                     LRIAQRLSEQRPDSLPPLSVCLQVNISGEATKGGVSLAAAPTLAQAVAVLPRLRLRGL
                     MAVPAPSGDFMTQRQPFRCLRELSERLQADGLQLDTLSMGMSHDLEAAIAEGATLVRV
                     GTAIFGERNTA"
     gene            4519..>5123
                     /locus_tag="PLL14_03745"
     CDS             4519..>5123
                     /locus_tag="PLL14_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG03066.1"
                     /translation="MDITELLAFSVKNKASDLHLSAGLPPIIRVNGDVRRINLPAMEH
                     KDVHAMVYDIMSDVQRKQYEDTRECDFSFEIPNLARFRVNAFVQNRGAGAVFRTIPSK
                     VLTLEELNCPKIFKDISDYPRGIVLVTGPTGSGKSTTLAAMINHVNENSYSHILTVED
                     PIEFVHQSKKCLINQREVGPHTLSFQNALRSALREDPDVILV"
BASE COUNT          990 a         1659 c         1506 g          868 t
ORIGIN      
        1 gaaggtgccc ttgttgcggg gaaaaagcgt cttcaacgtc ttcttcgaaa attccacgcg
       61 cacgcggacg actttcgaaa tcgccgccaa acggctgtct gcggacgtga tcaaccttga
      121 tgtcacgcgt tcttcgaccg ccaagggcga aaccctgctg gacacggtcg acaacctggt
      181 ggccatgcat gccgacatgt tcgtcgtcag gcacgcggcc agtggcgcgc cgcacctgat
      241 cgccgaccat ctgcgccgcc tcggacgcaa cgatgtgcat gtggtcaacg ccggcgatgg
      301 cagccacgcg catccgacgc aggggctgct cgacatgtat acgattcgcc actacaagaa
      361 ggatttcacc aacctggcgg tggcgattgt cggcgatatt ctgcattcac gggtcgcccg
      421 ttcggatatc cacgcattga ccacccttgg cgccgccgaa gtgcgcgctg tcggcccaca
      481 gacgctgctc ccgaccgcca tcgagaagat gggcgtgcgt gtctcccacg atctgggcac
      541 cggtcttcgc gattgcgatg tggtgatcat gctgcgcctg cagaacgagc gcatgaatgg
      601 cgccctgctg ccctcggcgc gcgagtactt tcgctgttac ggcctgacgc cggccatgct
      661 ggcgctggcc aaaccggacg ccattgtgat gcaccccggg ccgatgaacc gcggcgtgga
      721 aatcgacagc gatgtcgccg acggaccaca ggcagtgatc ctgccgcagg tcaccttcgg
      781 gattgccgtc cggatggcgg tcatgggaat cctggcgggg aactgagcga tgaacagaca
      841 acacctgcac ctgcgcaatg ctcgcctgat cgacccgaaa atcagtcttg atgcccacct
      901 cgacctgttc attgccgatg gcctggtggc gggcatcggc cagccaccga ccggtttcgc
      961 ggctgaacgc agcatcgacg ccagcggcct gatcgcctgc cccggtctgg tcgacctctc
     1021 cagccggctt ggcggcatcg aacccgaact caacgcagca gtcgccggcg gcgtcacttc
     1081 gctcgcctgt ccgccagaca gcaaaccacc gctcgacgaa ccgggcctcg tcgaacggct
     1141 ggttcgacgc agcgagacgc tcggcctggc acgcgtttat cccatcggcg ccctgacaca
     1201 gcaactggcc ggcgagaaac tcgccgaaat gaacagtctg gcgcgcgccg gctgcatcgc
     1261 tttttcgcaa gccaaacggg cgatcgtcga cacccagttg ttgttgcggg cgatgcaata
     1321 cgccgccact ttcggctatg ccgtgcgctt gcggccgcag gatcagtttc tcaccggcga
     1381 cggcgttgcc cacgatggcg aggtggcttc acggctcggg ctggccagca ttccggtatc
     1441 ggccgaaacc gtcgccatcg ccacggcact gctgctggca tcccagaccg gggttcgcct
     1501 gcatctttca cgcctctcgt cggccgcagg catcgccctg attcgccagg cacgacggtc
     1561 caacgtgcag gtcagttgtg acgtcgcggt ccatcacctg cacctgtcgg aaatggacat
     1621 cggctacttc gacagcaatg cccgcttcga cccgccactg cgttcggcga gcgaccgtga
     1681 cgcactgcgc gccgcagttg cggaaggtct ggcggcgata tgttcggacc ataccccggt
     1741 tgatgcggac ggcaagcaac tgccgtttgc cgaagccctg cccggcgcga ccggactcga
     1801 actcctgctg ccactgaccc tgcagtgggc ggcgcaggaa aaactgccgc tgtctctggc
     1861 gctggcccgc atcacctgcg atccggccgc gattctcggc atcgaagcag gttcgctgag
     1921 tgtcggcagt cccgccgacg tctgcgtctt cgatcccgac gaatcctggc gcgtcacccc
     1981 ggaggccttg cgcagccagg gcaagaacac cccgttcatg ggttacgaag tcagcggtcg
     2041 agtgcgcatg acannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacccgca
     2161 gcgacttgcg tcgtgaggtt aggccacttt tcagttccac cgccgagcgt gcgaggccca
     2221 gccgctgggc gatgaacgcc agcagtgcgg cgttggcccg gccgtctacc gccggcgcgg
     2281 cgaggcggat tttgagggca tcaccgtact caccggccac ctcgctgcgc ttcgctcccg
     2341 gctgaacatg aatggtcaga atgatcgcgc cgtcaacctc gcgtagccag tcgggcatca
     2401 cgctacagaa agataagcag cagttgggca aggacgatgg caatcagcgg cgacagatca
     2461 atggcgccaa tcgggggcac cacgcgctgg atcggttgca ggacgggtct tgtcaattgc
     2521 gacaccggct ggctgaatgg ggaataggga ttgacccaag acagcaccgc ctgcgccaat
     2581 agtgcaccaa tcagcaggta gaggctgagg cgcaaggtcg ccacgacgcc acgccacagc
     2641 agcaggggaa tcagtacctc cagcgaccag gcttccatcg cgatgccgcc acgcagcacg
     2701 aacaccgcgc aggcaaacag gacctgcagc acataagccg gcagcaggct tgccagatcc
     2761 agcccgaaca atcccggtag caccttgcgc agcggcctga ccagccagtt ggtcaactcg
     2821 acgacgaagg tgccgatctg gttgttgaac gagacgcgac aggcctgcat gtagaaacgc
     2881 aggagcagtg caaaagtcag aaaaccagtc agcgcttcaa tcagaaacag gctggcctgg
     2941 atgatcatca ggcgtccccg ccaagcagtt cgcccaactc gcgaccacgc gcattggcgg
     3001 caaagacccc ggcgatgaaa ccctccttga caccgcgctc ggccatcgtc cgcaatgccg
     3061 cctcggtggt gccgcctttg gaggtcactc gctcccgcag cacgctggcc gcttcgtcgg
     3121 actgcgaggc cagcttggcg gccccgagta cggtatcgat cgaaagttga cgggcctgtg
     3181 ccggcgtcag tcccagttcc ttcgctgcct gctgcagggc ctcgatgaac aggaagacat
     3241 aggccggccc actccccgag atggcggtca cggcgtccat ctgcgcttcg tcggcaatcc
     3301 acagggtact gccgaccgcc tgcagaatac gctcggcacc gagtcgctcg gccatcgaca
     3361 ccgccggcaa ggcatacagg ccggtaacgc cggcgccaat caacgcgggg gtgttgggca
     3421 tcgcgcgcac gatcttgccg taaccaccca gccagcgtga aacgtcagcc aggcgcagac
     3481 cggcagcgat gctgatgatc aattgctgct tgagatggcg aagcagcggc tcacaagcgt
     3541 cccgcaactg ctgcggcttg acggacagca gcaccgcatc gcagtcgagc gccgccgccg
     3601 tcggcgcttc gtaacagcgc acaccgtaca ccaaccgcag cttggctcgc ccctgcgcac
     3661 cgagttcgat gacggcgatg tcggctgccg aaaaaccggt cttcaccagg ccgccgatca
     3721 tcgcgttggc catattgcca ccgccaagga aagtgatctt catgctgtgt ttctctcacc
     3781 aaaaattgcg gtgccgacac gcaccagcgt cgctccttcg gcaattgctg cttccaggtc
     3841 gtgcgacata cccatcgaaa gtgtatcaag ctgcagacca tcggcctgta atcgctcgga
     3901 aagttcccgc aaacagcgga aaggttggcg ctgcgtcatg aaatccccgc tcggcgccgg
     3961 cacggccatc aggcctcgca ggcgtagtcg cggcagcacc gcgactgcct gtgccagggt
     4021 cggtgcagcc gcgagggaaa caccaccttt cgttgcctcg ccgctgatgt tgacctgcag
     4081 gcagaccgac aacgggggca gcgagtctgg acgttgttcg gagaggcgct gcgcaatccg
     4141 cagccggtcc accgaatgca cccagtcgaa agactcggcg acgatgcgcg tcttgttgct
     4201 ctgcagcgaa ccaatgaaat gccagtccag ccgcagcgaa cgtaacgcgg agatcttgtc
     4261 aaccccttcc tgaacatagc tctcgccaaa tgcccgctgg ccggcggctg ccagttcagc
     4321 caccgacgcg gagggccacg tcttgcttac agcaagcagc agtaccttgg cgggatcacg
     4381 gccgtgctgc tgcgcagcgc gcgcgattcg cgcgagaacg tcttgcagcc tggcaccaag
     4441 tgtggtcata atcggatgtt gcgcaaattt acgagagtat agcaccgcgc cctgcccacc
     4501 gcccgaggac cagcacggat ggacatcacc gaactacttg cgttcagtgt caagaacaag
     4561 gcttccgacc tccatctgtc tgcgggcctg ccaccgatca tccgggtcaa cggtgacgtg
     4621 cggcggatca acctgccggc gatggagcac aaggacgtgc acgccatggt ctacgacatc
     4681 atgagcgacg tacagcgcaa gcagtacgag gacacgcggg aatgcgattt ctcgttcgag
     4741 attcccaatc tggcgcgttt ccgggtcaac gctttcgtcc agaatcgcgg tgccggggcg
     4801 gtattccgaa cgattccgtc gaaggtgctg acgctcgaag aactgaactg cccgaagatc
     4861 ttcaaggaca tctccgatta tccgcgaggc atcgtgctgg tcaccgggcc taccggctcg
     4921 ggcaagtcga ccaccctggc ggcgatgatc aatcatgtca atgaaaattc gtatagccac
     4981 atcctcaccg tcgaggatcc catcgagttt gtgcaccagt cgaagaaatg cctgatcaac
     5041 cagcgtgagg tcggcccgca tacactgtct ttccagaacg ctctgcgctc ggccttgcgc
     5101 gaagatccgg acgtgatcct ggt
//
LOCUS       DAOSIT010000102         5112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14570_length_5112_cov_9.425549, whole genome shotgun sequence.
ACCESSION   DAOSIT010000102 DAOSIT010000000
VERSION     DAOSIT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14570_length_5112_cov_9.425549"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2514
                     /locus_tag="PLL14_03750"
     CDS             <1..2514
                     /locus_tag="PLL14_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOG03067.1"
                     /translation="DILGESRRDLRFRVTLMVTGGLVAPGPRGRGSPPATPHYAAHLL
                     IGAMAAPQQAQTVEAIRCYADLRPTAVAAGGNTPRVLFGPELATDAEKPSESSIRLDG
                     LPFGEALAGLLELACAEETRAIVASEVFGVWINRGCPVASLQFSACWQGRRALVSHTY
                     GLAPDAAPPAWLDPQRGGTVDPGLFHSVFLPVRKLIEIGLLTTFSDDRSRPMINKLGH
                     KVAAIANMAKLAAKTPHGSRWEKLLSALTAVQAWSEQVESQEKGRLLEVIGFGTNPGE
                     LRMYSYVPERLPANAPLVVVLHGCTQSAASYNKGSGWSTLADRYGFALLLPQQQWNNN
                     PLRCFNWFKPEDNERDKGEPLSIRQMVDRMLHDHGLDRRQVYVTGTSSGGAMTTVMLA
                     TYPEVFAGGAVLAGVPYRTAKGLQEGLESIFQGRSRSAGEWGQLVRAASSHQGPWPKV
                     SVWHGDADTAVKPVNAEEIIKQWADVHGLDAQPTIQMTVDGHSRRAWQGPSGEELIES
                     YTVLGMGHGAALDPGAEPHQCGTAAPFFNAVGISSTHRIADFWGLLTDRPASAAGLVQ
                     AEPAAAAGQGGHDARALDRGERGVKEHAARDTDPPRGSAAPGQRAAAGRPFGLDVHGI
                     ISASLEAAGLLKAGTTNRRPDSSAPLGIDVPGIISTALEAAGVLKGIAQRSASAQPEV
                     ALKTAGWEGAGWELLLEDPRAFRGGPMLYGHAASGDHGALGARAQSISRRIKLGPQPE
                     LSYVRRLDLSAGVNDYTSASFRVLIDGIVVDEVTAIGMVHQETEWLRQAAIDLKQFAD
                     RSVTLTLEVAACANIYSTVYAKAWVDQITIHNAVDLAHC"
     gene            complement(2585..3361)
                     /locus_tag="PLL14_03755"
     CDS             complement(2585..3361)
                     /locus_tag="PLL14_03755"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOG03068.1"
                     /translation="MSYVNINAEVRGKVGLITLNRPKQLNALNDDLMDELGSALATFE
                     ADENIGAIVITGSDKAFAAGADITVMQSLTYMEAYKGDFITRNWDSLKSCRKPVIAAV
                     AGYALGGGCELAMMCDIILAADNAQFGQPEVRLGILPGAGGTQRLPRAISKAKAMEMC
                     LASRNIDATEAERAGLVSRVVPADKLLDEAMATATRIASFSLPVVMMIKECINRSYES
                     SLSEGLLFERRTFHSAFALEDQKEGMAAFVEKRKPSFSNR"
     gene            complement(3607..5073)
                     /locus_tag="PLL14_03760"
     CDS             complement(3607..5073)
                     /locus_tag="PLL14_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal transporter"
                     /protein_id="HOG03069.1"
                     /translation="MFALLREMPDFTTSWWRYMLAMMRASNREVSEFNDNLFIAAHIA
                     MTAARDNPLSMLEAFEIVEHNVGMARKGMAGMQGKMADYAFDQVEEGTRAFLNTVLKA
                     DGEKLGGYMRREAEIMESVSNFNEQIEAIADEFGFHFNTPNYKLVHETDAFELYQVLP
                     LKAGVEVRKDLKPVILVPPYMLGVHILAFLPFEGKSYSHAFANEGIPTYVRVVKDIMT
                     NEKVQTLTPEQDCEQTRQLCAKAMELNDGKKVTLNGTCQGGYISLMNILSGKLKDVCD
                     ALITNVTPVDGTYSDAISGMPTMHHDFITTTLPSGNKVANGYLLSLGMRFVAIDRETP
                     LVKVLDQVSLHRATDLNPGKTPAALFRWLLKERVHLPLAIANMSSHTFQDPIANDGTL
                     PVKLFDQPLNIKNLIELNVPWYQNYAIKDDLVTPPCATAGNKFLEGWDRLESVAFFGG
                     HVAILTSPHGKKAPVTGSFTDANGKASRGPVKFQIDIS"
BASE COUNT          878 a         1537 c         1693 g         1004 t
ORIGIN      
        1 gatatcctcg gggaatcgcg tcgcgacctg cgctttcgcg tcacgctgat ggtcactggc
       61 ggcctggtgg cgccggggcc gcgcgggcgg ggctcaccac cggcaacgcc gcattacgcg
      121 gcgcatctgc tgatcggggc catggccgcg ccacaacagg cccagaccgt ggaggccatt
      181 cgttgctatg ccgacttgcg gccgacggcg gtggccgccg gcggcaacac cccgcgagtt
      241 ttgttcggcc cggaactggc aacggatgcc gagaaaccgt ctgaatcgtc gatacgtctg
      301 gacggtctgc cgttcggtga ggcgctggcc ggcctgctgg aactggcgtg tgccgaagag
      361 acgcgcgcga ttgtcgccag cgaggtgttt ggcgtgtgga tcaaccgtgg ctgcccggtt
      421 gcctcgctgc agttcagcgc ctgctggcag ggccggcgtg cgctcgtcag tcatacctat
      481 ggcctggccc cggacgccgc tccgccggcc tggcttgacc cccaacgcgg tggcacggtc
      541 gatcccggcc tgttccactc cgttttcttg ccggtccgca agctgatcga gatcggtctg
      601 ctcaccacct tctccgatga caggagtcgt ccaatgatca acaaactggg ccataaagtg
      661 gccgccatcg ccaacatggc aaaactcgcg gccaagacgc cgcacggaag tcgctgggaa
      721 aagctccttt ccgccctgac ggcggtccag gcgtggtcgg agcaggtgga gtcgcaggag
      781 aagggccgcc tgctggaagt gatcggtttc ggcactaatc ccggcgaact gcggatgtat
      841 agctacgtcc ccgaacggct gccggccaac gcgccgctgg tcgtcgtgct gcacggctgt
      901 acgcaaagcg cagcctcgta caacaagggc agcggctggt cgacgctcgc cgatcgctac
      961 ggtttcgcgc tcctgctgcc gcagcagcag tggaacaaca acccgctgcg ctgcttcaac
     1021 tggttcaagc cggaagacaa cgagcgtgac aagggtgagc cgttgtcgat ccggcagatg
     1081 gtcgaccgga tgttgcacga tcatggtctc gatcgccggc aggtctacgt taccggtact
     1141 tcgtcgggcg gcgccatgac cacggtcatg ctggcgacct atccggaggt gttcgccggc
     1201 ggcgccgtgc tcgccggggt tccttaccgt accgccaagg gtttgcagga agggctcgag
     1261 agcatcttcc aggggcggtc tcgatcggcc ggggagtggg ggcaactggt gcgcgcggcg
     1321 tcgtcccacc aggggccatg gccgaaagtc tcggtctggc atggtgacgc cgataccgcc
     1381 gtcaagccgg tcaatgccga agaaatcatc aagcaatggg ccgacgtgca cggcctcgat
     1441 gcgcagccga cgatccagat gacggttgac ggccattcgc gccgggcctg gcaggggccc
     1501 agcggcgaag aactgatcga gtcctacacg gtacttggca tgggccacgg cgctgccctt
     1561 gatccgggcg ccgagcccca ccagtgcggt acggcggcgc cgttcttcaa cgcggtaggg
     1621 atttcatcga cacaccggat cgccgatttc tggggattgc tcaccgaccg gccggccagt
     1681 gcagccggtc tcgtgcaggc cgaaccggca gcggcggctg ggcaaggagg ccatgacgcc
     1741 cgcgcactcg accgcggcga gcggggggtc aaggaacatg ccgcgcgcga caccgatccg
     1801 ccacgtggtt cagcggcacc ggggcagcgt gccgcggccg gccgaccctt cgggctcgat
     1861 gtccatggca tcatctccgc gtcgctggaa gcggctggcc tgctcaaggc gggcaccacg
     1921 aaccgacgcc cggacagttc ggcgccactg gggatcgacg ttcccgggat catttcgacg
     1981 gcactggaag cggcaggcgt gctgaaaggt attgcgcagc gttctgcttc ggcgcaaccc
     2041 gaagttgcgc tcaagaccgc cggctgggaa ggtgcgggat gggaactgct gctcgaagac
     2101 ccgcgtgcat ttcgcggtgg gccgatgctc tatggtcacg ccgcatcggg tgatcacggt
     2161 gccctcggcg cacgcgccca gtcgatctca cggcggatca aactcggtcc gcagccggaa
     2221 ctgagctacg tgcgacgcct cgatctcagc gctggcgtca atgactacac cagcgcgagt
     2281 ttccgcgtcc tgatcgacgg tattgtcgtc gatgaagtca cggcgatcgg catggtgcac
     2341 caggagaccg aatggctgcg ccaggcggcg atcgacctga agcagtttgc cgatcgcagc
     2401 gtcacgctga ctctcgaagt ggcggcttgc gcgaacatct acagcacggt ttacgccaag
     2461 gcgtgggtgg accagatcac gatccataat gccgtcgatc tggctcattg ctgagctctg
     2521 acggatccac cggatcccct ggcgatgttt ccggggctcc ggtgggttat gtacggcgat
     2581 caggtcagcg gttggagaac gagggtttgc gcttttcgac gaatgccgcc atgccttcct
     2641 tctggtcttc cagagcaaaa gccgagtgga acgtgcggcg ttcgaagagc agaccttcgg
     2701 aaagcgagct ctcgtaggaa cggttgatac actccttgat catcatcacg accggcagcg
     2761 agaagctggc gatgcgggtg gcggtggcca tcgcctcatc gaggagcttg tcggccggga
     2821 cgacccgtga aaccagaccg gcgcgctcgg cttccgtggc gtcgatgtta cgcgaggcca
     2881 ggcacatctc catggccttg gccttggaaa tcgcccgcgg caggcgctgc gtgccaccgg
     2941 cgccggggag aattcccagg cgaacttcgg gctggccaaa ttgcgcgttg tcggcagcca
     3001 ggatgatgtc gcacatcatc gccagttcgc agccgccgcc gagtgcgtag ccggcaaccg
     3061 cagcaatcac cggtttgcgg cagctcttga ggctgtccca gttgcgggtg atgaagtcgc
     3121 ccttgtaggc ttccatgtaa gtcagggatt gcatgacggt aatgtcggcg ccggcagcaa
     3181 aggccttgtc cgagccggtg atgacgatcg cgccgatgtt ttcgtcggcc tcgaaggtgg
     3241 ccagagcgct gcccagttcg tccatcaggt catcgttcaa ggcattcagt tgcttggggc
     3301 ggttgagagt gatcaggccg acttttccgc gcacttcggc gttgatgttg acatagctca
     3361 taagggaaat ctccaggttc agagtggatg gtgtcgagat cccgccaatt gtcgcgggat
     3421 ttccttgctg cggtgatcgg aatcggtgct ggacgtgcgg ctgcgcgtgt tgcgcctggg
     3481 caggggtgac acgccgacgc gccggcctga acaggttgct gtcaggccgg cgcgtcgttt
     3541 gctcaaggca ggggtgcagt gatcagcggt gccgcgtctt cagttcccga agggcttgtg
     3601 tgccaatcag gaaatgtcga tctggaattt gactggtccg cgcgacgctt tgccgttggc
     3661 gtcggtgaag gagccggtga ccggggcctt tttgccgtgc ggactggtca ggatggccac
     3721 gtgcccgccg aagaaggcga ccgactcgag acggtcccaa ccttcgagga acttgttgcc
     3781 ggcggtggcg cacggtggcg tgacgaggtc gtccttgatc gcgtagttct ggtaccacgg
     3841 cacgttcagc tcgatcaggt tcttgatgtt caacggctga tcgaacagtt tcaccggcag
     3901 ggtcccgtcg ttggcgatcg gatcctggaa ggtgtgcgaa ctcatgttgg caattgccag
     3961 cggcaggtgc acacgttcct tgagcaacca gcggaacagc gctgccggcg tcttgccggg
     4021 gttcaggtcg gtggcccgat gcagcgaaac ctgatcgagc actttcacca gcggggtttc
     4081 ccggtcaatg gcgacgaagc gcattcccag gctcagcagg tagccgttgg cgaccttgtt
     4141 accgctcggc agcgtcgtgg tgatgaaatc gtggtgcatc gtcggcatgc cgctgatggc
     4201 gtcgctgtag gtgccgtcaa ccggggtcac attggtaatc agggcgtcgc agacgtcctt
     4261 cagtttgccc gacagaatgt tcatcagcga gatgtaaccg ccctggcagg tgccgttcag
     4321 cgtgactttc ttgccgtcgt tgagttccat ggccttggcg cacagttggc gggtctgttc
     4381 gcagtcctgc tccggcgtca gcgtctggac cttctcgttg gtcatgatgt ccttgacgac
     4441 gcggacgtag gtgggaatgc cttcgttggc gaacgcgtgg ctgtagctct tgccctcgaa
     4501 cggcaggaac gccaggatgt gtacgcccag catgtagggc ggaacgagga tgaccggctt
     4561 cagatccttg cgaacttcga cgccggcctt cagcggcagc acctggtaga gctcgaaggc
     4621 gtcggtttca tggaccagct tgtagttcgg ggtgttgaaa tggaatccga actcgtcggc
     4681 gatggcctcg atctgctcgt tgaaattcga aaccgattcc atgatttcgg cttcgcgacg
     4741 catgtagcca cccagtttct cgccatccgc cttcaggacg gtgttcagga acgcgcgtgt
     4801 gccttcctcg acctgatcga aagcgtagtc ggccatcttg ccctgcatgc cggccatccc
     4861 tttgcgggcc atgccgacgt tgtgctcgac gatctcgaaa gcttccagca tgctcagcgg
     4921 gttgtcgcgc gccgcggtca tggcaatgtg tgcggcaatg aagaggttgt cgttgaattc
     4981 gctgacttcg cggttcgagg cgcgcatcat cgccagcatg tacctccacc aactcgtggt
     5041 gaaatcaggc atttccctta gcagtgcaaa catcttccaa cctccaacaa tttacgtcac
     5101 ttagaacggg cc
//
LOCUS       DAOSIT010000103         5109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14578_length_5109_cov_8.778195, whole genome shotgun sequence.
ACCESSION   DAOSIT010000103 DAOSIT010000000
VERSION     DAOSIT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14578_length_5109_cov_8.778195"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLL14_03765"
     CDS             complement(<1..869)
                     /locus_tag="PLL14_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HOG03070.1"
                     /translation="MNFQQLRIIRETLRQDFNLTEVANALYTSQPGVSKHIKDLEDEL
                     GIEIFVRRGKRLLGLTEPGKELAEVVDRILFDTQNLRRIADQFASRETGHFVVATTHT
                     QARYALPQIIKWFKTDYPRVHLALHQGSPVDIAEKLVSGEADVGIATERLDRVPELAT
                     FPFYSWHHALIVPHGHPLLQVEKLTLEQIGEYPIITYHEGFTGRTHIDKAFADAGVMP
                     DIVLSAIDADVIKTYVELDLGVGIVASMAYDPVKDRNLALIQAPHLFPESTTRLAVRR
                     GIYLRSYALAFIE"
     gene            complement(879..1946)
                     /locus_tag="PLL14_03770"
     CDS             complement(879..1946)
                     /locus_tag="PLL14_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HOG03071.1"
                     /translation="MSIAIRNISKQFGAFKALDDVSIDIPSGELVALLGPSGCGKTTL
                     LRIIAGLDFADQGQILLEGDDASARHVRDRHVGFVFQHYALFRHMSVADNVAFGLRVK
                     PRRLRPSEVEIKRRVKRLLELVQLDWLADRYPSQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRQLHDEIHVTSVFVTHDQEEALEVSDRVVVINKGKVEQI
                     GTPESVYDHPATPFVANFLGSVNLFHGRVENGHLHVGDDALALGSTDGSDGQAVAFVR
                     PHEFDLVPGWEARGGLPARIVRIVSVGPSAQIELTHHDGELIEVTIGREAFRLLGLSE
                     GDQVSLRPRHIRVFPSEAQAA"
     gene            complement(1950..2843)
                     /gene="cysW"
                     /locus_tag="PLL14_03775"
     CDS             complement(1950..2843)
                     /gene="cysW"
                     /locus_tag="PLL14_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOG03072.1"
                     /translation="MSASIALHAGSLERQETASTREPAWVKVVLVSVALSFFSVFLLL
                     PLGTVFAEALRKGWGAYFDAMVNPDAWSAIKLTLLVAVIAVPLNLVFGLAASWAIAKF
                     EFRGKRVLITLIDLPFSVSPVISGLIYVLMFGAQGWFGPWFYEHDIKIIFAVPGIVLA
                     TIFVTFPFVARELIPLMEAQGSEEEEAAVVLGASGWQVFRYTTLPNIKWGLLYGVILC
                     NARAMGEFGAVSVVSGHIRGQTNTMPLHVEILYNEYNYAAAFAVASLLAVLAIITLGL
                     KTLVERQTRRQLQDIDTPSGE"
     gene            complement(2845..3681)
                     /gene="cysT"
                     /locus_tag="PLL14_03780"
     CDS             complement(2845..3681)
                     /gene="cysT"
                     /locus_tag="PLL14_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOG03073.1"
                     /translation="MASLRRFSPLPGFGLAMGYTLVYLAIIVVIPLTAVGAKTFTLGW
                     DAFWAAVSSPRVVASYQLSFGASLLAAGINVVFGLIFAWVLVRYRFPGKGLVDSLVDL
                     PFALPTAVAGIALATIYSQNGPVGKLLEPLGVEIAYTPKGVLLALVFIGLPFVVRTVQ
                     PVLADLEKEVEEAAAGLGASRWQTFCKIILPHIAPALLTGFAMAFARAVGEYGSVIFI
                     AGNVPMVSEITPLMIITKLEQYDYAGATAVASVMLIISFVLLLIINGLQGWMQNRYAK
                     GA"
     gene            complement(3859..4869)
                     /locus_tag="PLL14_03785"
     CDS             complement(3859..4869)
                     /locus_tag="PLL14_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG03074.1"
                     /translation="MKVQRLFAGLLAGALLLGSANAAEIAILNVSYDPTRELYQDFNA
                     AFSRYWQGKTGDTVKVNASHGGSGKQARSVIDGIDADVVTLALAYDIDAIADRGILPL
                     DWQKRLPNNAAPYTSTIVFLVRKGNPKAIKDWSDLIKPGVAIVTPNPKTSGGARWNYL
                     AAWAYALKQSGGSEAKAREFVGQIYGNVKVLDSGARGSTTTFVERGIGDVLIAWENEA
                     YLAIKELGPDKFEIVTPSLSILAEPPVSVVDKVVDKRATRKVAQAYLEYLYSPEGQDI
                     AARHYYRPRDAKIAAKYARQFGKVNLVTIDDTFGGWRNAQKTHFSDGGIFDQLYVPGG
                     KR"
BASE COUNT         1005 a         1548 c         1620 g          936 t
ORIGIN      
        1 ttttcgatga acgccagggc gtagctgcgc aggtagattc cccgccgaac ggcgaggcgg
       61 gtcgtgcttt caggaaacag gtgtggtgcc tgaatcagcg ccaggttgcg gtccttgacg
      121 gggtcgtagg ccatcgacgc gacgatgccg acgccgagat cgagctcgac gtaggtcttg
      181 atcacatcgg cgtcgattgc cgataacacg atgtcgggca tcacgccagc gtcggcaaaa
      241 gccttgtcga tgtgcgtccg gccggtaaac ccctcatgat aggtgatgat cggatattca
      301 ccgatctgtt ccagagtcag cttctcgacc tgcagcagcg ggtggccgtg cggaacgatc
      361 agcgcgtggt gccagctgta gaagggaaag gtcgccagtt ccgggaccct gtccagacgc
      421 tcggtagcga taccgacatc ggcttcgccc gacaccagtt tttcggcgat gtccaccgga
      481 cttccctgat gcagcgccag gtgcacgcgt ggatagtcgg tcttgaacca cttgatgatc
      541 tgcggcagcg cgtaacgcgc ctgggtgtgg gtggtggcca ccacgaagtg gccggtttcg
      601 cggctggcaa actggtcggc aatgcggcgc aggttctggg tgtcgaacag aatgcggtcg
      661 accacctcgg cgagttcctt gcccggctcg gtcaacccca gcagacgctt gccgcggcgc
      721 acgaagatct cgatcccgag ttcgtcctcg agatccttga tgtgcttgct gacccccggc
      781 tgcgaggtat acagcgcgtt ggccacttcg gtcaggttga aatcctgacg cagggtctcg
      841 cgaatgatgc gcagttgctg aaagttcatt gccttgcttc aggccgcctg ggcttcgctg
      901 gggaagaccc ggatgtggcg cgggcgcagc gatacctggt ctccttccga gagccccagc
      961 aggcgaaatg cctcgcggcc aatggtcacc tcgatcagtt cgccgtcatg gtgcgtgagc
     1021 tcgatctggg cgctcgggcc gacggaaacg atgcgcacga tgcgcgccgg cagcccccct
     1081 cgcgcctccc agccgggcac caggtcgaac tcgtgcggcc gcacgaaagc caccgcctgg
     1141 ccgtcgctgc catcggtcga gccaagtgcc agcgcgtcat cgccaacatg caggtgccca
     1201 ttttcgacac ggccgtggaa cagattgacc gaaccgagga agttggcgac gaaaggcgtc
     1261 gccggatggt cgtagaccga ttccggcgtc ccgatctgtt cgaccttgcc cttgttgatc
     1321 accaccaccc ggtcggaaac ctccagggcc tcctcctggt cgtgcgtgac gaagacgctg
     1381 gtcacgtgga tttcgtcgtg cagctgccgt agccagcgtc gcaattcctt gcgcaccttg
     1441 gcgtcgaggg caccgaaagg ctcgtcgagg agcaacacct tgggttcgac cgccagcgcg
     1501 cgcgccagag caatccgctg gcgctggcca ccggaaagct gtgacggata acggtccgcg
     1561 agccagtcga gttgcaccag ttcgagcagg cgcttgaccc gccgtttgat ttcgacttcg
     1621 ctgggacgca gtcgccgcgg tttgacgcgc aggccgaagg cgacattgtc ggcaacgctc
     1681 atgtggcgga acaatgcgta gtgctggaag acgaagccca cgtgccggtc acgcacgtga
     1741 cgggccgaag cgtcgtcgcc ttcgagcagg atttgaccct ggtcggcgaa gtcaagaccg
     1801 gcgatgatcc gcagcagcgt cgtcttgccg cagccggagg gcccgagaag agcgaccagc
     1861 tcgccggaag ggatgtcgat cgagacgtcg tcgagcgcct tgaaggcgcc gaactgcttg
     1921 ctgatgttac ggatcgcaat actcatcaat cattctccgg aaggggtgtc gatgtcctgc
     1981 aattggcgac gtgtctgacg ttcgaccagc gtcttcaggc ccagggtgat gatcgccagc
     2041 acggccagca gggaggccac ggcaaaggcc gcggcgtagt tgtattcgtt gtagagaatc
     2101 tcgacgtgca ggggcatggt gttggtctgg ccgcggatgt gcccggaaac caccgacacc
     2161 gcgccgaact cgcccatcgc gcgcgcgttg cagaggatca cgccgtacaa caatccccat
     2221 ttgatgttcg gcagcgtggt gtatcggaag acctgccagc cggacgcacc gagaacgacg
     2281 gccgcctcct cttcctcgct cccctgcgct tccatcaacg gaatcagttc ccgggcgaca
     2341 aacggaaagg tgacgaagat tgtcgccagg acgatgcccg gcacggcgaa aatgatcttg
     2401 atgtcgtgtt cgtagaacca ggggccaaac cagccctgcg cgccgaacat cagcacgtag
     2461 atcagacccg agatcaccgg cgataccgaa aagggcagat cgatcagggt gatcaacacg
     2521 cgcttgccgc gaaattcgaa cttggcaatg gcccaggaag cggccaatcc gaacaccagg
     2581 ttcagcggca cggcgatcac cgcgaccagc aaggtcagct tgatcgccga ccaggcgtca
     2641 gggttgacca tggcgtcgaa atacgcgccc cagcccttgc gcagagcttc ggcgaaaacc
     2701 gtgcccaagg gcaacagcag gaagacgctg aagaaagaca gcgcgacgct gaccagtacc
     2761 accttgaccc aggccggttc gcgggtgctt gcggtttcct ggcgctccag gctgcccgca
     2821 tgcagtgcaa tactcgcgct catttcaggc tcctttggcg tagcggttct gcatccatcc
     2881 ctgcaggccg ttgatgatca gcaggaggac aaaggagatg atcaacatca ccgaagccac
     2941 ggcggtggca ccggcgtagt cgtactgctc cagtttggtg atgatcatca gtggtgtgat
     3001 ttcggagacc atcggcacat tgccggcgat gaaaatgacc gagccgtatt ctccgaccgc
     3061 ccgcgcaaag gccatggcga agccggtcag caaggcgggc gcgatgtgcg gcaggatgat
     3121 cttgcagaag gtctgccagc gcgaggcgcc gagaccggcg gcggcttcct cgacttcctt
     3181 ttcgagatcg gcgagcaccg gctgcaccgt gcgcacgaca aacggcagac cgatgaatac
     3241 cagcgccagc agcacgccct tgggggtata ggcaatttcg acacccaggg gctcgagcag
     3301 cttgccgacc ggaccgttct gcgaatagat cgtcgccagg gcgatgcccg ccaccgctgt
     3361 cggcagcgca aagggcagat cgaccagcga atcgaccagg cccttgccgg gaaagcggta
     3421 gcgcaccaga acccaggcaa agatcagtcc gaagacaacg ttgatgcccg ccgccagcaa
     3481 ggaagcgccg aagctcaatt gatacgaggc caccacgcgc ggactgctca ccgccgccca
     3541 gaaggcatcc cagccgaggg tgaaggtttt cgcgcccact gccgtgagcg gaatgacgac
     3601 gatgatggcc aggtagacca gggtgtagcc catcgccagg ccaaaccccg gcaaggggct
     3661 gaatcgcctc aggctggcca tgcggaactc ccgaataaca cttggatcat tctcaactcc
     3721 tgctgcaacg caacatgaca tgccgccagt ctatgggcaa gcaagttatg atgagaaata
     3781 cttaaatgtg agaacgtaat gcaaattcgt tataaagacg ggactgtatg ccggtagccc
     3841 cgccccgtcg acgagaactc agcgtttgcc gcctggaacg taaagctggt cgaagatgcc
     3901 accatcggag aagtgggtct tctgggcgtt gcgccagccg ccaaaggtgt catcgatggt
     3961 caccaggttg accttgccga actgcctggc gtacttcgcc gcgatcttgg cgtcgcgcgg
     4021 gcggtagtag tgcctggcgg caatgtcctg gccttcgggg gaatacaggt attcgaggta
     4081 ggcctgcgcg accttgcgcg tggcgcgttt gtcgaccacc ttgtcgacca ccgacaccgg
     4141 cggctcggcg aggatcgaca gcgagggcgt gacgatttcg aacttgtccg gaccgagttc
     4201 cttgatcgcc agataggctt cattctccca ggcgatcagg acatcgccga tgccccgctc
     4261 gacgaaggtg gtggtcgagc cgcgcgcgcc ggaatcgagg accttgacgt tgccgtagat
     4321 ctggccgacg aattcgcggg ctttcgcttc gctgcccccg gactgtttca gcgcgtaggc
     4381 ccaggccgcc aggtagttcc agcgcgcgcc accggacgtc ttggggttgg gggtgacgat
     4441 ggcgacaccc ggcttgatca gatcgctcca gtccttgatg gccttgggat tgcctttccg
     4501 gaccaggaaa acgatcgtcg aggtgtaggg cgccgcgttg ttgggcaggc gcttctgcca
     4561 gtcgagcggc aagatgcccc ggtcggcgat ggcgtcgatg tcgtaggcca gagccagggt
     4621 gaccacgtcc gcgtcgatgc cgtcgatcac cgatcgggcc tgcttccccg aaccgccgtg
     4681 cgaagcgttg accttgaccg tgtcgccggt cttcccttgc caatacctgg aaaatgccgc
     4741 gttgaaatcc tgatacaact cgcgtgtcgg gtcgtaggaa acattcagga tggcgatctc
     4801 ggcggcattc gcactgccga gcagcagggc gccggccagc aggccggcga acagacgttg
     4861 cactttcatg gggactccgt tcagtcaagg ggtggcgcgg agtctagaaa agcggcgcgg
     4921 cgaaacgaac gaatctttgc ttattagctt atacgcaggt tttccgatgc ctcggtttgc
     4981 cgaccgccat actcatttcc ggtgtcggct tcctggttgg gtagcacggt ataaccgtgg
     5041 gtctggagca catgcatggc tttctccagt tttttcacgt ctttcagcgc agcagcagga
     5101 tccgccgca
//
LOCUS       DAOSIT010000104         5098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14621_length_5098_cov_11.238747, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000104 DAOSIT010000000
VERSION     DAOSIT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14621_length_5098_cov_11.238747"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..990)
                     /gene="cbiB"
                     /locus_tag="PLL14_03790"
     CDS             complement(25..990)
                     /gene="cbiB"
                     /locus_tag="PLL14_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOG03075.1"
                     /translation="MPNVFSFPFLAAGMPTVLSLPLAAIAGAGLDRLLGEPRRWHPLV
                     GFGALAGAVEGALNGGASPRRCRLRGLLAWALLVLPLPLLVVWSMPSGLSGALIDAAL
                     LYLALGARSLSEHARRVADDLASGDLDAARRHVGWMVSRQTTDLDAAGVAGACIESTL
                     ENGNDAVFGTLFWFALFGGAGALLFRLANTLDAMWGYRDQRFADFGCAAARIDDLLNF
                     VPARLTALSYALCGRARDALRCWRRQAPSWKSPNAGPVMAAGAGSLGLLLGGAAVYHG
                     RLEQRPVLGEGRPAGGQDIARALTLVRRSLLLWLAVPLCWGLLRA"
     gene            1025..2506
                     /locus_tag="PLL14_03795"
     CDS             1025..2506
                     /locus_tag="PLL14_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOG03076.1"
                     /translation="MNAHRTPSLMIQGCTSDAGKSVLVTALCRILHRRGVRVAPFKPQ
                     NMALNSAVTVDGGEIGRAQALQALAAGLPAHSDFNPVLLKPATDKRAQVIIHGQALRD
                     LDALDYHAYKPLAMAAVLESWQRLGAQYECLLVEGAGSPAEINLRDRDIANMGFAEAV
                     DCPVILIADIDRGGVFAHFCGTLDLLSASEQARVKGFVINRFRGDIGLLESGLSWLEE
                     RTGKPVLGVLPYLHGLYLDAEDALPRQTENKAAAALRVVSPVYPRISNHNDLDPLRFH
                     PEVDFRFVGPNDPPPPCDLIVLPGSKAVQADLAWLRAQGWEEAIRRHLRYGGKLIGIC
                     GGLQMLGRALHDPLGLEGVAGSMPGLGWLDYETQLAAEKTLENVSGELLLPGSPAVSG
                     YTIHMGVTRGAALASPAAVLAGQPDGAMSDNGQILATYCHGLFDAPPALTALLTWAGH
                     RPTQDFDPRQRRENDIDRLADAVEQHLNLRLLAEWLPARAIRR"
     assembly_gap    2681..2780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2781..4558
                     /locus_tag="PLL14_03800"
     CDS             <2781..4558
                     /locus_tag="PLL14_03800"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG03077.1"
                     /translation="VGSAGDTLGSAGTGCTLAPGIAEAGFSGHRRLAVPTLPRLHNLT
                     EENGAMQARRPVQAASTLCACLLALAPAAALAATPAPAATRLALVIGNAAYRVAPLDN
                     PVGDARAIAERLREAGFRVTLKLDAPRRELQEAIRGFGDALAEDNRAVGVFYYAGHGV
                     QLNWRNFMLPVDARIHRPADIAAQGVDVGLLLESLGRARNPLNLVILDACRDNPFGSD
                     FRSSERGLSQLDAPPGTLLAYATAPGNTAGDGTGAHGLYTGHLLKEMQVPGAAVEDVF
                     KRVRLAVRRASEGAQIPWESTSLEGDFAFVGDAKRDLAREQREFEADLTAWNVLRSTS
                     TPEPLEAFIRQRPSGKFAELAQHRLDLLLRERGEKPVDAAPPPPLGPEMCVPGKDAPY
                     GGPAVPFLPGERYTYRSVDLLSQAETARTTDRVLDIVGDEVRFNDGNKVSDLFGNNVR
                     APDGKRWTPYQFFINDYALGKRWRAQFIITQPDGTTVGVRFDLRVAARERITLPAGTF
                     DSYRLEARGSDLASGAQLERSAWVAPERMRGVLAMESIVRKGGKVVSAERVELTEHVA
                     GVSPAPARAMATEPVDKPPRSPLAY"
     gene            complement(4593..>5098)
                     /locus_tag="PLL14_03805"
     CDS             complement(4593..>5098)
                     /locus_tag="PLL14_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03078.1"
                     /translation="GGLVAACFAFPAQAQPGPAGMGGGMGGMGPGPQATAVDCRKASN
                     PQHCLDRQQAQTACKDLRGPARQDCMTDHMPPPDCGKSADPARCAAMQSARTACKGKY
                     GPERQACLQENPVPPPSPGTRGGGMGPGTQGMGPGMGGMGGKGGMGPCGGAGTAPCPL
                     APAPAKP"
BASE COUNT          808 a         1821 c         1650 g          719 t
ORIGIN      
        1 gccgcgcgca ggcgcccgcc gtgttcaagc acgcagcaat ccccagcaca gcggcaccgc
       61 cagccacagc aggaggctgc ggcgaacgag ggtcagcgcg cgggcaatgt cctggccgcc
      121 cgccggccgg ccttcgccga gcaccggccg ctgctccagc cggccgtggt acaccgccgc
      181 accgccgagc aggaggccca gactgccggc acccgcggcc atcaccgggc cggcgttcgg
      241 gctcttccag ctcggcgcct gccgccgcca gcaacgcagg gcgtcgcgcg cgcggccgca
      301 aagggcatac gacaaggccg tcagacgggc ggggacaaag ttcaacaggt cgtcgatacg
      361 cgcggccgca cagccgaagt cggcaaaacg ctggtcgcga tacccccaca tcgcgtcgag
      421 cgtattggcc agtcggaaca gcagcgcccc cgctccgccg aatagcgcaa accagaacag
      481 cgtgccgaat accgcatcgt tgccgttctc gagtgtcgat tcgatgcagg cgccggcgac
      541 accggcggca tcgaggtccg tggtctgccg cgaaaccatc cagccgacgt gccggcgcgc
      601 cgcgtcgaga tcgccgctcg ccagatcgtc ggccacccgg cgggcgtgtt cgctgaggct
      661 gcgcgcgccg agcgccaggt aaagcagggc ggcgtcgatc agagcgcccg acaaccccga
      721 tggcattgac cacaccacca gcagcggcag gggcagaacc agcaaagccc aggccagcag
      781 cccacgcagg cgacaccggc gaggcgacgc cccaccgttc aaagcacctt ccacggcacc
      841 cgccagcgca ccgaaaccca ccagggggtg ccagcgccgc ggctcaccga gcagacgatc
      901 gagtccggct ccggcaatcg ccgccagcgg cagggacagc accgtcggca tgccggcagc
      961 gaggaaagga aaggaaaaca cgttgggcat gcgacaatcc gggccacgaa acccggattc
     1021 tacgatgaac gcgcaccgca cccccagcct gatgattcag ggctgcactt ccgacgccgg
     1081 caagagcgtg ctggtcaccg ccttgtgccg catcctgcat cgccgcggcg tacgcgtcgc
     1141 gccgttcaaa ccgcagaaca tggcgctcaa ttcggcggtc accgtcgatg gcggcgagat
     1201 cggccgtgcg caggcactgc aggcgctggc cgccggtctg ccggcgcact ccgacttcaa
     1261 tccggtgctg ctgaagccgg ccaccgacaa gcgcgcgcag gtgatcatcc acggccaggc
     1321 gctgcgcgac ctcgacgcgc tcgactatca cgcctacaag ccgctggcga tggccgccgt
     1381 gctcgaatcg tggcaacgcc tcggcgcgca gtacgagtgt ctgctcgtcg aaggggccgg
     1441 cagcccggca gagatcaatc tgcgcgaccg cgacatcgcc aacatgggtt ttgccgaggc
     1501 cgtcgattgc ccggtgatcc tgatcgccga catcgatcgc ggcggcgtct tcgcgcattt
     1561 ctgcggcacg ctcgatctgt tgtcggcctc cgagcaggcg cgcgtcaagg gtttcgtgat
     1621 caaccgcttt cgtggcgaca tcgggctgct cgagagtggc ctctcctggc tcgaagaacg
     1681 caccggcaaa ccggttctcg gtgtgctgcc gtatctgcat ggcctctacc tcgacgccga
     1741 ggatgccctg ccacggcaga ccgaaaacaa ggcggcggcc gctctgcgcg tcgtgtcgcc
     1801 ggtctatccg cggatctcca atcacaacga tctcgacccc ttgcgcttcc accccgaagt
     1861 cgattttcgt ttcgtcggcc caaacgaccc gccaccgccc tgtgacctga tcgtgctgcc
     1921 gggctcgaaa gcggtacagg ccgacctcgc gtggttacgc gcgcagggct gggaagaggc
     1981 gatccgtcgc cacctgcgct acggcggcaa gctgatcggc atctgcggcg gcctgcagat
     2041 gctcggccgc gcactgcacg atccgctcgg actcgaaggg gtcgcgggca gcatgcccgg
     2101 cctcggctgg ctcgactacg aaacgcaact ggccgcggaa aagacgctgg agaatgtcag
     2161 cggcgaatta ctgctgcccg gatcgccagc ggtcagcggc tacaccatcc acatgggcgt
     2221 cacgcgcggc gcggcgctgg ccagtccggc ggcagtcctc gccggccagc ccgatggcgc
     2281 catgtccgac aacgggcaga ttctggccac ctactgccac ggcctgttcg atgcaccgcc
     2341 ggcgctgacc gcactgctca cctgggccgg ccaccgtccg acacaggact tcgatccgcg
     2401 ccagcgccgg gaaaacgaca tcgaccgcct ggccgacgcc gtcgagcaac acctgaacct
     2461 gcgcctcctc gccgagtggc tgccggcgcg ggcgattcgc cggtgaaacc tcgcggtcgc
     2521 cgccctggta gccggatttt cccaattgcc gcagtgtcgt aatggccatc aggccgaatt
     2581 cggccgtgca accatcgggc ggcttgtcgc aacccttatt gaccgccgtc ggcagcgccg
     2641 gagatactct cgggtctgcc ggcacgggtt gcacgcttgc nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn ccgtcggcag cgccggagat actctcgggt ctgccggcac
     2821 gggttgcacg cttgcccccg gcatcgccga agcagggttt tcaggccatc gccgtctggc
     2881 ggtgccgaca ctgcctcgtt tgcacaacct cacggaggag aacggagcca tgcaagcacg
     2941 ccgccctgtc caagccgcca gcacactctg cgcctgcctg ctggcgctcg cacccgccgc
     3001 cgcactggcc gcgacacccg cgccggcagc gacgcgactt gccctggtca tcggcaacgc
     3061 ggcctaccgg gtggcgccgc tcgacaatcc cgtgggagat gcccgggcga tcgccgagcg
     3121 tctgcgcgag gcgggtttcc gcgtgacgct caaactggat gcgccgcggc gcgagctgca
     3181 ggaggcgatc cgcggcttcg gcgatgcgct ggccgaggac aaccgcgccg tcggtgtctt
     3241 ctattacgcc ggccacggcg tccagctcaa ctggcgcaac ttcatgttgc cggtggacgc
     3301 gcgcattcat cggccggcgg acatcgcggc gcagggtgtc gatgtcggcc tgttgctgga
     3361 gagtctcggc cgcgcgcgca atccgctcaa cctggtgatc ctcgacgcct gccgcgacaa
     3421 ccccttcggc agcgatttcc gcagcagcga gcgcggcctt tcgcaactcg acgcgccacc
     3481 cggcaccctg ctcgcctacg ccacggcgcc gggcaatacc gccggcgacg gcaccggcgc
     3541 gcatgggcta tataccgggc acctgctcaa ggaaatgcag gtgcccggcg cggccgtcga
     3601 ggatgtcttc aagcgcgttc gcctggccgt tcggcgggcc tccgaagggg cgcagatccc
     3661 ttgggagagc acttcgctgg aaggcgattt tgccttcgtc ggcgacgcca aacgcgacct
     3721 cgcccgcgag cagcgggagt tcgaagccga tctcacggcc tggaatgtcc tgcggtcaac
     3781 gagcacgccc gagccgctgg aagcgttcat ccgtcagcgg ccgagcggca agttcgctga
     3841 actggcgcag catcggctcg acctgctgct gcgcgaacgc ggcgaaaaac ccgtcgatgc
     3901 cgcgccaccg ccgccgctcg gcccggagat gtgcgtcccc ggcaaggacg cgccctacgg
     3961 tggtccggcg gttcccttcc tgcccggcga gcgctacacc tatcgcagcg tcgatctcct
     4021 gtcgcaggcc gagacggcac gcacgaccga ccgcgtcctc gacatcgtcg gcgacgaggt
     4081 gcgcttcaat gacggcaaca aggtgagcga tctcttcggc aacaacgtgc gggcgcccga
     4141 cggcaagcgc tggacgccct accagttctt catcaacgac tatgcgctcg gcaaacgctg
     4201 gcgcgcgcag ttcatcatca cccagcccga cggcacgacc gtcggcgtgc gtttcgacct
     4261 gcgcgtcgcc gcgcgcgagc gcatcaccct gcccgccggc acattcgaca gctatcgcct
     4321 cgaagcccgt ggcagcgacc tcgccagcgg cgcgcaactc gagcgcagcg cctgggtcgc
     4381 ccccgagcga atgcgcggtg tgctggcgat ggagagcatc gtccgcaagg gcggcaaggt
     4441 ggtcagcgcg gagcgcgtcg aactcaccga gcacgttgcc ggcgtgagcc ccgcgccggc
     4501 gcgcgccatg gccaccgagc ccgtggacaa gccgccgcgt tctccgttgg cctactgagc
     4561 cggccgcctg gaagcggcgg cggtggcccg gatcagggct tggccggcgc tggggcaagt
     4621 ggacagggcg ctgtccctgc gccgccgcag gggcccatgc cacccttgcc acccatgcca
     4681 cccattcccg gacccatccc ttgcgtgccg gggcccatcc cgccgccgcg ggtccctggc
     4741 gatggaggtg gcacagggtt ttcctgcagg caggcctgcc gttcggggcc atacttgccc
     4801 ttgcacgcgg ttcttgccga ctgcatggcg gcgcagcgcg cgggatcggc agatttgccg
     4861 caatcgggcg gcggcatgtg gtcggtcatg cagtcctgcc gggctggtcc gcgcagatcc
     4921 ttgcaggcgg tctgcgcctg ctgccggtcg agacagtgtt gcgggttgct ggccttgcgg
     4981 caatcgaccg cagtcgcctg cgggccggga cccatgccgc ccattcctcc gcccatgccg
     5041 gcagggccgg gctgggcctg cgccgggaac gcgaagcagg cggcgacgag accgccga
//
LOCUS       DAOSIT010000105         5087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14662_length_5087_cov_8.016097, whole genome shotgun sequence.
ACCESSION   DAOSIT010000105 DAOSIT010000000
VERSION     DAOSIT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14662_length_5087_cov_8.016097"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLL14_03810"
     CDS             complement(<1..633)
                     /locus_tag="PLL14_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03079.1"
                     /translation="MSRASNIYRLGIKELWSLLRDPIMLVLIAYSFTFQVYIAATAMP
                     ETLSNVPIAIVDEDDSPLSARITSALRPPQFMPPTRVTLPEVDVGLDAGLYTFSLNIP
                     PNFQRDVLAGRAPAIQLNVDATRMSQAFTGSGYIQQIVMGEINEFVQRYRGNATPPVD
                     LALRMRFNPALTRAWFGSLMEIINNVTMLSIVLAGAALIREREHGTIEHLL"
     gene            complement(636..3392)
                     /gene="rbbA"
                     /locus_tag="PLL14_03815"
     CDS             complement(636..3392)
                     /gene="rbbA"
                     /locus_tag="PLL14_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HOG03080.1"
                     /translation="MSGAPSGLVARLSGVSLRYAKTIALDAVDLAVPAGCMVGLIGPD
                     GVGKSSLLALISGARRVQQGTVEVLGGNMQSRRHRDRVCPAIAYMPQGLGKNLYPALS
                     VEDNLQFFGRLFGHNAVERRRRIDELTKATGLHAFLDRPAGKLSGGMKQKLGLCCALI
                     HDPDMLILDEPTTGVDPLARTQFWSLIAHIREARPGMSVMIATAYMEEAERFDWLVAM
                     DAGRILTTGSPKELHERTGTATLEAAFIALLPEEKKRGHRAVVVPPFADGENVAIEAR
                     DLTMRFGDFTAVDRVNFRIHRGEIFGFLGSNGCGKTTTMKMLTGLLPPSEGEAWLFGQ
                     KVDARDITTRRRVGYMSQAFSLYSELSVEQNLELHARLFSVPESAIPSRVSELLTRFG
                     LDGVPDAMPDKLSLGMRQRLSLAVALVHAPELLILDEPTSGVDPVARDAFWQFLVDLS
                     RTDKVTIFISTHFMNEAERCDRISLMNDGQVLASDAPEEIVKKRGARTLEEAFIAYLK
                     DADPADAEDIPDTTTWAPVSAASQGISAHPRMRHRGFNFARAFSYTLREALELRRDPV
                     RLVMALFGSLILMLIMGYGITMDVNDLSYAVLDRDQTSLSQNYALNLSGSRYFLEHPP
                     ITDYADLDRRMRSGELSLAIEIPSGFARDVQRGTPVQIGAWIDGAMPQRAETVQGYVQ
                     GMHQGWLIDIAKTRFGQDIAAGAASIETRFRYNPDVKSLPAMVPAVIPLLLMMIPAML
                     TALSVVREKELGSIINLYVTPVTRSEFLIGKQIPYIVLAMLNFLLMTLLAVTLFGVPV
                     KGSFMTILLGTLIYVGFSTGFGLFASTFTRSQIAAMFFAMIGSMLPAIQFSGMINPVS
                     TLEGAGRLVGEIYPATYYITISRGVFNKALGFADLQVTFVPMLVAAVVILCLSIALLK
                     KQEK"
     gene            complement(3439..4431)
                     /locus_tag="PLL14_03820"
     CDS             complement(3439..4431)
                     /locus_tag="PLL14_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG03081.1"
                     /translation="MTRPKGLGEGFVSGNGRIEATEIDVASRYPGRVVEIFVFEGDFV
                     QAGQVLAQMQIDVLTAQRDEARAQQQQAITGVASAEAQARMRESELAAAQAIVVQRAS
                     ELDNAKRRLARSQTLSREGATSIQQLDDDQAAVYNAQAALAAAEAQAKAAAAAIVASK
                     SQVTGARAAIEAATATIARIDADIKDSQLKAPRDGRVQYRVAQPGEVLGAGGKVLNMV
                     DLSDVYMTFFLPETVAGKVALGSEVRLVLDAAPGSVVPARVSFVASVAQFTPKTVETA
                     VERQKLMFRVKARIDPELLKKHLKQVKTGLPGMAWIKLDRQAEWPESLKIKVAE"
     gene            complement(4548..>5087)
                     /locus_tag="PLL14_03825"
     CDS             complement(4548..>5087)
                     /locus_tag="PLL14_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG03082.1"
                     /translation="ANIGAARAAFFPRITLTGSAGTASAGLDGLFDSGSRAWNFLPSL
                     SLPIFDAGRNRANLDLAEVRRNLAVTNYEKTVQAAFRDVSDALSARHWLTEQVRIAQA
                     TLAAQTERARLSQLRYDNGSTPYFEVLDAQRDLLTAEQQLVQTRRALLSSRVSLYAAL
                     GGGSQEFAAPVDTATHSAN"
BASE COUNT          993 a         1540 c         1558 g          996 t
ORIGIN      
        1 cagcaggtgt tcgatggtgc cgtgctcgcg ttcgcggatc agcgcggcgc cggccagcac
       61 gatggatagc atggtgacgt tgttgataat ctccatcagc gagccgaacc aggcccgcgt
      121 caaggccggg ttaaagcgca tccgcaaggc cagatccacc ggtggcgtgg cattgccgcg
      181 atagcgctga acgaattcgt tgatttcgcc catcacgatc tgctggatgt aaccgctgcc
      241 ggtaaaggcc tggctcatgc gcgtggcatc cacattgagc tggatagcgg gcgctcgccc
      301 cgccagcacg tcgcgctgga agttcggcgg aatattgagc gaaaaggtat agagccccgc
      361 atccaagccg acatcgacct cgggcagcgt cacccgcgtt ggcggcatga attgcggcgg
      421 acgcagagcc gaggtgatac gtgccgatag cggcgagtca tcctcgtcga cgatcgcgat
      481 cggcacattg ctgagcgtct ccggcatggc ggtggcggcg atgtagacct gaaaagtgaa
      541 gctgtaggcg atcagcacca gcatgatcgg atcgcgcagc aggctccaca gctccttgat
      601 gcccaaacgg tagatgttgg atgctcggct cataattatt tttcctgttt tttcaatagg
      661 gcgatggaca ggcagaggat aaccacggcc gccacgagca tcggcacaaa tgtcacttgc
      721 agatcggcaa accccagtgc cttgttgaac acgccgcggc tgatagtgat gtagtacgtg
      781 gccgggtaaa tttcaccgac cagccggcct gcgccttcga gcgtcgagac cgggttgatc
      841 atgccggaga actggatggc tggcagcatg gatccgatca tggcaaagaa catggccgca
      901 atctggctgc gcgtaaaggt ggaggcgaac agaccgaagc cggtggagaa accgacatag
      961 atcagcgtcc ccagcagaat ggtcatgaag ctgcccttga ccgggacgcc gaacagggtc
     1021 acggccaaca gggtcatcag cagaaaattg agcatggcca gcacgatgta agggatttgc
     1081 ttgccgatca ggaattcgct gcgggtcacg ggcgtgacat agagatttat gatcgagccc
     1141 aactcctttt cgcgcactac ggatagcgcg gtaagcatgg ccgggatcat catcaacaac
     1201 aggggaatta cggccggcac catcgccggc aggctcttca cgtccgggtt gtagcgaaag
     1261 cgtgtctcga tgctggccgc accggctgca atgtcctgac cgaagcgggt tttggcgata
     1321 tcgatcaacc agccctggtg catgccctgc acataaccct gcacggtttc tgccctctgc
     1381 ggcatcgcgc cgtcgatcca ggcgccgatc tgcaccgggg taccgcgctg cacgtcgcgc
     1441 gcgaagcccg acggaatttc aatggccagc gacagttcgc cgctacgcat gcgccggtcg
     1501 aggtcggcat agtcggtaat cggcgggtgc tctagaaaat agcgcgaacc ggagaggttc
     1561 aaggcatagt tctggctgag gctggtctgg tcgcggtcca gcacggcgta gctaagatcg
     1621 ttcacatcca tggtgatgcc gtagcccatg atgagcatca ggatcaggct gccgaacagc
     1681 gccatcacta aacgcaccgg gtcacgccgc agttccagcg cctcacgcag ggtatagctg
     1741 aaggcgcgcg caaaattgaa gccgcgatgc cgcatccgcg ggtgcgccga tatgccttga
     1801 gacgcggcag aaacaggcgc ccaggtcgtg gtatcgggta tatcctctgc atcggccggg
     1861 tctgcatcct tcaaataagc gataaaagct tcttccagcg tcctggcgcc gcgctttttg
     1921 acaatctcct ctggcgcgtc gctggccagc acctgcccgt cattcattag cgaaatgcgg
     1981 tcgcagcgct cagcctcatt catgaagtgg gtcgagatga agattgttac cttgtcagtg
     2041 cgcgacagat cgacaaggaa ctgccagaag gcatcgcgtg ctactggatc aacacccgag
     2101 gtcggttcgt ccaggatcag cagttccggt gcatgcacca gcgctaccgc cagcgacagg
     2161 cgctggcgca tgccgagcga caatttgtcg ggcatggcat cgggcacgcc gtccagccca
     2221 aaacgagtca aaagttcgct tacccgcgac gggattgccg attctggcac gctgaacagg
     2281 cgcgcatgca gctccagatt ctgctcgacg ctgagttcgg agtacagcga aaacgcctgc
     2341 gacatatagc cgacacgccg tcgcgtggtg atgtcgcgcg cgtccacttt ctgtccgaac
     2401 agccaggctt cgccctcaga cggtggcaag agaccggtga gcatcttcat ggtggtggtc
     2461 ttgccacaac cgttggagcc gaggaagccg aaaatctcac cgcggtggat gcggaagttg
     2521 acgcggtcga ccgccgtgaa atctccgaag cgcatggtca ggtcgcgcgc ttcgatggca
     2581 acattctcgc cgtcggcaaa cggcggcacc acgactgccc gatggccgcg ttttttttct
     2641 tccggcagca gtgcgatgaa ggccgcttcc agcgtcgcag tgccggtgcg ttcgtgcagt
     2701 tctttgggcg atcctgtggt gagaatccgt cccgcgtcca tcgccaccag ccagtcgaag
     2761 cgttctgcct cctccatgta agcggtggcg atcatcacgc tcatgcccgg acgcgcttca
     2821 cggatatggg caatcagtga ccagaattgc gtgcgcgcca gcggatcgac cccggtggtt
     2881 ggttcatcaa gaatcagcat atccgggtcg tggatcaggg cgcagcacaa gcctaatttc
     2941 tgcttcatcc cgcctgacag tttgcctgcc ggacgatcaa ggaaagcatg gaggcccgtc
     3001 gctttcgtca gttcgtcgat gcgccgtctg cgttcaaccg cattgtggcc gaacaggcga
     3061 ccgaagaatt gcagattgtc ttcgaccgac agggccgggt agagattctt gcccagacct
     3121 tgtggcatgt aggcaatggc ggggcatacc cggtcgcggt ggcgtcttga ttgcatgttg
     3181 ccgccaagca cctccacagt gccttgctgc accctacgcg cgccggaaat caaggccagc
     3241 agactggact tgccgacgcc atccgggccg atcagcccga ccatgcagcc agcgggcacc
     3301 gccagatcaa cggcatccag cgctatcgtc ttggcatagc gcaggctgac accggacagg
     3361 cgcgcgacca ggccacttgg cgcgcctgac atatttacat gggtggtgct ggatgtggca
     3421 agtgtcactt agccgccctc actcagcgac cttgatcttg agcgactcag gccactcggc
     3481 ctgccggtcc agtttgatcc aggccatgcc gggcaggccg gtcttgacct gcttcaggtg
     3541 ttttttcagc aactctggat cgatgcgggc tttcacccgg aacatgagct tttgtcgttc
     3601 aaccgcggtt tccactgttt tcggcgtaaa ttgcgcaaca ctcgcgacga acgacactcg
     3661 cgcgggaacc acagagccgg gcgcggcatc cagtacaaga cgcacctcgc tgcccagcgc
     3721 tacttttccg gccaccgtct cgggcaggaa gaatgtcata tagacatcgc tcagatccac
     3781 catattgagt accttgccgc cggcacccag cacttcgcca ggctgggcca cgcgatactg
     3841 tacgcggcca tcgcgcggcg ccttgagctg gctgtccttg atgtccgcat cgatgcgcgc
     3901 aatcgtcgcg gtggcggcct cgatggcggc gcgtgcgcct gtgacttgtg acttgcttgc
     3961 cacaatggcc gctgcggccg ctttcgcctg ggcttcggcc gcagccaatg cggcttgggc
     4021 gttatacact gccgcctgat cgtcgtctag ttgctggatg gatgtcgcgc cttcacgcga
     4081 tagcgtttgc gaacgagcca ggcgccgctt ggcgttatcc agctcactcg cgcgctgcac
     4141 cacgatggct tgcgccgccg ccaactcact ttcacgcatc ctcgcttgcg cttcggcgct
     4201 cgctacgccg gtaatcgcct gttgctgctg ggcgcgcgcc tcatcgcgct gcgcggtgag
     4261 tacatcgatc tgcatttgtg ccagcacctg acctgcctgc acgaaatccc cttcgaagac
     4321 gaaaatctcg accacgcgcc ccggatacct ggaggcgaca tcgatctcgg tggcttcgat
     4381 acggccattg ccgctgacaa agccttcgcc caggcctttc gggcgggtca tcgtccacac
     4441 gccccaggcg atcagcgcaa tgacaatggc cgccgcggcc aggaacaggt ttttcttgag
     4501 tgcagggctt aatggattca tggtgagttc tcgtgtgtgc tgttgtttta atttgccgag
     4561 tgggttgccg tatccaccgg ggcggcaaat tcctggctgc cgccgcccag cgctgcgtac
     4621 aggcttacgc ggcttgagag cagggcgcgg cgcgtttgta ccagttgctg ctcagccgtg
     4681 agcaggtcgc gctgggcatc caggacttca aaataagggg tgctgccatt gtcataacgc
     4741 aactgggata gccgcgcacg ttcggtttgc gccgccagcg tcgcctgtgc gatgcgcacc
     4801 tgttcagtca gccagtggcg ggcggataag gcatcggaca catcacggaa ggccgcctgc
     4861 acggtctttt cataattcgt caccgccagg ttgcgacgga cttcggccaa atcaaggttg
     4921 gcgcggttgc gcccggcatc gaaaatcggc agggaaaggc tgggcaggaa gttccaggcg
     4981 cggctgccgg aatcgaacag gccatcgagc ccagcgctgg cggtacccgc cgaaccggtc
     5041 aaggtaatgc gcggaaaaaa ggccgcgcgc gccgcgccga tgttggc
//
LOCUS       DAOSIT010000106         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14694_length_5080_cov_10.714428, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000106 DAOSIT010000000
VERSION     DAOSIT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14694_length_5080_cov_10.714428"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1467
                     /gene="atpD"
                     /locus_tag="PLL14_03830"
     CDS             118..1467
                     /gene="atpD"
                     /locus_tag="PLL14_03830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOG03083.1"
                     /translation="MIEEVHGPVIDILCSRLPPLHQAVYVCLDGERYIFEVHRHLDES
                     RARAIALHRTAGLRRLLPVFDSGRPLQIPVTPDCLGRLLDLFGHPLDGGPALASTEFR
                     PIVAPPAPLSEAMGMGEILPTGIKVIDLLCPFVRGGKTGLFGGAGVGKTVLIMEFMNA
                     IVRLHQGVSVFAGVGERIREGHELWHDMQDAGVMPRSLMVFGQMDESPGVRFRIGLSA
                     LTYAEYLRDHVAKEVLFLMDNAFRFVQAGSELSGLLGRMPATVGYQPTLLSEVAELQE
                     RISSTRSGNITAVEAVYVPADDMTDPAVGAILAHLDTTVILSRNQAAKGIYPAVDPLA
                     SGSRMMDRVTLGDRHYRVAQTVREHLARYRELEDIIAMLGIEALSEADRRTVLRARLL
                     ERYLTQPFFVVADHTGIAGASVPLATTLTDCEDFIQGTYDDLPEDRCYMRAGMEGVR"
     gene            1464..1868
                     /locus_tag="PLL14_03835"
     CDS             1464..1868
                     /locus_tag="PLL14_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOG03084.1"
                     /translation="MSGGITLNLQSALRCERIDKVQSFVGSDASGSFGIQPGRARFMT
                     ILDYGLSRFRSTQGAWQYLACPGAVLRFADDQLTISTRRYLCDEDYERISELLAGQLA
                     QEEEKLRSVKDNLQKLERELFRRLRTLDRWPS"
     gene            1891..2163
                     /locus_tag="PLL14_03840"
     CDS             1891..2163
                     /locus_tag="PLL14_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG03085.1"
                     /translation="MNDERWRREVSRDSERLKEAGRSRRSLLAQTVFLGSLSVLFLAP
                     LLAGAYLGRWLDSLGEGYTVRWTVNLILIGLALGVFNVYLFIRKYW"
     gene            2163..2873
                     /locus_tag="PLL14_03845"
     CDS             2163..2873
                     /locus_tag="PLL14_03845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG03086.1"
                     /translation="MEAASVVFSLGGLRITSTVVTTWGILMAFGLSCWLATRRLSVDH
                     PGLVQTALEGVVQSIEAAIEGVLPGRAGLLLPFVGTLWLFVAVANLTGIVPGLHAPTG
                     NLSTTAALACLVFVSVHWFGIRSEGLKNYLRHYLVPSPILLPFHLLGEFSRTIALAMR
                     LFGNIMSLEMAALLVLLVAGLLVPVPVLMLHIVEALVQAYIFGTLALIYIAGGMQSHA
                     AAEHSEAPSSNDTKGSTP"
     gene            2870..3136
                     /locus_tag="PLL14_03850"
     CDS             2870..3136
                     /locus_tag="PLL14_03850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734788.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; subunit C is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit C"
                     /protein_id="HOG03087.1"
                     /translation="MSDMHWFVLVSTVAAALAIAIGIIFPAIAMGRAITQALEALSRQ
                     PEAEKSITRTLFIGLAMIESLAIYVLVIVLIILFRNPLLEYLLK"
     gene            3146..3892
                     /locus_tag="PLL14_03855"
     CDS             3146..3892
                     /locus_tag="PLL14_03855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOG03088.1"
                     /translation="MDLDWTTFALEIINFLALIWILKRFLYRPVLATLAERRAGIEGS
                     LNEARATEARAAALQRQFESRLADWEREKAAARSQFDAQMAAEHARQMEALDRDLAAE
                     RQRSAAREAHRQETLQRELASQSGALARQFASALLARLAGPEIEARLVEIFVEELSAL
                     PEERLEPLRAGQNGHGQGVLSSAHPLSEEQRRQISGAVDARLGKLVNLVFVEDSELLA
                     GVRLSLGAWQLDLSLAGELGVFAEAGRLDH"
     gene            3882..>5080
                     /locus_tag="PLL14_03860"
     CDS             3882..>5080
                     /locus_tag="PLL14_03860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG03089.1"
                     /translation="MITEHSAPNPGGRGSQWLADYQPQLRISEIGKLAAIGDGIAWIE
                     GLPSAAMDQILRFENGSEALVFHLGSRRIGAILLGQEDGLAAGAAAHLTGRRLDIGVG
                     DGFLGRVVDPLGNPLDGGAQPQFSRRRPLEVSSPPITARDFVRRPLLSGSKMVDTMIP
                     IGKGQRQLIIGDAGTGKSSLAMDTIIAQKGSDVLCVYVLISQKRSAVVTTIETLRRAR
                     VLDISCVVVAEATTTPGLKFLAPFAGCALAEEWMWSGRDVLVVYDDLTTHARAYRELS
                     LLLRRPPGREAYPGDIFYLHARLLERSTCLAARYGSGSMTALPIVETEQGEIAAYIPT
                     NLISITDGQIYLDPALYARGFLPAIDITRSVSRIGGKAQLPAIKAEAGRMKLDFLQFL
                     ELEVFTR"
BASE COUNT          854 a         1671 c         1621 g          934 t
ORIGIN      
        1 gtatcggatc ggcattctgg ccgccagcaa gccatgacgg aagggctgct ggttttgcct
       61 tctgccatcg aaacggcaag gtcaccctgt cccatccccg agcaggcgcc gctcggggtg
      121 atcgaggagg tgcacggacc ggtcatcgac atcctgtgca gccgcctgcc accactgcat
      181 caggcggtgt atgtctgcct cgatggcgag cgctacatct tcgaggtgca tcgccatctc
      241 gacgaatcgc gggcgcgagc gatcgccctg catcgtacgg ccggtctgcg ccggctgctg
      301 ccggtcttcg atagtggtag gccgctgcag ataccggtga cccctgattg cctgggccgg
      361 ctgctcgacc tattcggcca ccctctggat ggcggcccgg cgctcgcgtc gacggaattt
      421 cgccccatcg tcgctccccc ggcaccgctc tcggaagcaa tgggcatggg agaaatcctg
      481 ccgaccggca tcaaggtgat cgacctcctg tgcccttttg tgcgcggcgg caagaccggc
      541 ctgttcggtg gcgcaggcgt tggcaaaacg gtgctgatca tggaattcat gaacgccatc
      601 gtccgtctgc accagggggt atcggtcttc gccggggtcg gtgagcgaat tcgcgaggga
      661 catgagttgt ggcacgacat gcaggacgcc ggcgtcatgc cgcgctcgct gatggtgttc
      721 ggccagatgg atgaatcgcc gggagtccgc ttccgcatcg ggctgtcggc actgacctac
      781 gccgaatacc tgcgagacca tgtcgccaag gaagtgttgt tcctgatgga caacgctttc
      841 cgcttcgtgc aggcgggcag cgagctgtcc gggctgctcg ggcgcatgcc ggcgacggtc
      901 ggctaccagc cgacactact ttccgaagtg gcggaattgc aggagcgcat ttcttccacg
      961 cgcagcggca acatcaccgc tgtcgaggcc gtctatgtac cggctgatga catgaccgac
     1021 ccggcagtcg gggcgatcct cgcccacctc gacaccaccg tcatcctctc gcgcaaccag
     1081 gcggccaagg ggatctaccc ggcggtcgac ccactagcgt cgggcagtcg catgatggat
     1141 cgcgtcaccc tcggcgaccg tcactatcgg gtggcgcaaa cggtgcgcga gcatctcgcg
     1201 cgctaccgcg aactggaaga catcatcgcc atgctgggca tcgaggccct gtcggaagcc
     1261 gaccggcgca ccgtgctgcg ggcgcgcctg ctcgaacgct atctcacgca gccgttcttt
     1321 gtcgttgccg accataccgg catcgccggg gcctcggtcc ctctggcaac gaccctcacc
     1381 gattgcgagg atttcatcca gggaacctac gacgacctgc ccgaggatcg ctgctacatg
     1441 cgcgccggca tggagggcgt gcgatgagcg gcgggatcac cctgaatctg cagagcgcgc
     1501 tgcgctgcga gcgcatcgac aaggtgcaga gctttgtcgg cagcgatgcc agcggcagct
     1561 tcggcattca gccgggacgc gcccgcttca tgacgatcct cgactatgga ctgtcgcgtt
     1621 ttcgcagcac gcagggtgcg tggcagtatc tcgcctgccc cggcgccgta ttgcgctttg
     1681 ccgacgatca actgaccatc agcactcggc gctacctttg tgacgaggac tacgagcgga
     1741 tctcggaact gctggcaggg caactggctc aggaagaaga aaaactccgg agcgtcaagg
     1801 acaatctgca gaaactggag cgggagcttt tccggcgctt gcgcaccctg gatcggtggc
     1861 cgtcatgaac gaccatcacc gcaagtcatt atgaacgacg aaagatggcg tcgcgaggtt
     1921 tcccgcgaca gcgaaagact caaggaggcc ggccggagtc ggcgctcgct gctcgcacag
     1981 acggtcttcc tcggcagtct gtcggtgctg ttcctggcgc ccttgctggc aggcgcttat
     2041 ctcgggcgct ggctggatag cctgggtgag gggtacacgg tgcggtggac ggtgaatctg
     2101 atcctcatcg gcctcgcgct cggcgtcttc aacgtctatc tcttcatccg aaaatactgg
     2161 taatggaagc ggcaagcgtt gttttcagtc tgggaggctt gcggatcacc tcgacggtgg
     2221 tcaccacctg gggcatcctg atggccttcg gactgtcctg ctggctggcg accagacgac
     2281 tctcggtcga ccaccccggt cttgtgcaaa ccgcgctgga aggtgtcgtc cagtcgatcg
     2341 aggcggccat cgaaggcgtg ctaccgggtc gggccggcct gctgctgcct ttcgtcggca
     2401 ccctctggct gttcgtcgct gtcgccaatc tgacgggaat cgtgcccggt ctgcatgcgc
     2461 cgaccggcaa cctgtcgacc accgccgcgc tggcctgtct ggtctttgtc tcggtgcact
     2521 ggttcggcat ccgcagcgaa ggcctgaaga attatctgcg ccactacctg gtccccagcc
     2581 cgatcctcct gccgtttcat ctgctcggcg agttttcccg caccatcgcg ctggcgatgc
     2641 gcctgttcgg caacatcatg agcctcgaaa tggccgcgct gctggtcctc ctggttgccg
     2701 ggctgctggt gccggtcccg gtgcttatgc tgcacatcgt cgaagccctg gtccaggcct
     2761 acatcttcgg cacgctggcc ctgatctaca tcgccggcgg catgcaatcc cacgccgctg
     2821 cggagcactc cgaagcgccc tcatcgaacg acacgaaagg aagcacccca tgagcgacat
     2881 gcactggttt gtcctggtct ccaccgtggc agcagcgctg gccatcgcga tcggcatcat
     2941 cttcccggcc attgccatgg gccgggcgat cacgcaggcg ctcgaagcgc tgtcgcggca
     3001 gccggaggcg gagaagtcga tcacccgcac cctgttcatc ggcctggcga tgatcgagtc
     3061 gctggccatc tacgtcctgg tgatcgtgct gatcatcctc ttccgcaatc cgctgctcga
     3121 atacctcttg aagtaggagg cggcgatgga tctggactgg accacctttg ccctcgaaat
     3181 catcaatttc ctggcgctga tctggatcct caagcgcttt ctctaccgcc cggtgctggc
     3241 gaccctggct gagcgtcgcg ctggcatcga gggctccctc aacgaagcac gcgcgacgga
     3301 agcacgggcc gcggccctgc agcgccagtt tgaaagccgt ctggcggact gggagcgaga
     3361 aaaggcggca gcccgcagcc agttcgatgc ccagatggcg gcagagcacg cgcgacagat
     3421 ggaggcgctg gaccgggatc tggctgccga gcggcaacgt agcgccgccc gcgaagcaca
     3481 ccggcaggag accctgcagc gcgaactcgc cagccagtcc ggcgcactcg cccgccagtt
     3541 cgccagcgca ctgctggcca gattggcagg accggagatc gaagcgcggc tggtagaaat
     3601 tttcgtcgag gaactctcgg ctctgcccga agaacgtctg gagcctttgc gggcgggcca
     3661 gaatggtcat gggcaagggg tgttgagcag cgcccacccg ctctcggagg aacaacgccg
     3721 gcagatctcc ggtgccgtcg atgcccggct gggaaagctc gtcaacctcg ttttcgtcga
     3781 ggatagcgag ttgctggccg gcgtccggct atcgctcggg gcctggcaac tggatctcag
     3841 tctggccggc gaactgggcg ttttcgcgga ggcaggcagg cttgatcact gagcattctg
     3901 ccccaaaccc tggtggccgg ggcagccagt ggctggccga ctaccagccg caattgcgga
     3961 tcagcgaaat cggcaagctg gcagcgatcg gcgacggcat cgcctggatc gaggggctgc
     4021 cctcggcggc gatggaccag atcctgcgtt tcgagaatgg cagcgaggcg ctggtgttcc
     4081 acctcggcag caggcgcatc ggcgccatcc tgctcggtca ggaggatggc ctcgccgccg
     4141 gcgcggcggc ccacctgacc ggacggcgac tggatatcgg cgtcggtgac ggctttctcg
     4201 gccgcgtggt ggacccgctg gggaatcccc tcgatggcgg tgcgcaaccg caattcagcc
     4261 gccggcgccc cctcgaagtc tcctcgccac cgatcaccgc ccgcgatttc gtcaggcgcc
     4321 cattgctgag tggcagcaag atggtcgata ccatgatccc gattggtaaa ggccagcggc
     4381 aactgatcat cggcgacgcg ggcaccggca agagttcgct ggccatggac acgatcatcg
     4441 cccagaaagg gagcgatgtt ctctgcgtct atgtgctgat cagccagaag cgctcggcgg
     4501 tggtgaccac catcgagacc ctgcgccggg cccgcgttct cgacatcagc tgcgtcgtcg
     4561 tcgccgaggc caccaccacg cccgggctca agttcctcgc gcccttcgcc ggctgcgccc
     4621 tggcggaaga atggatgtgg agcggccgcg acgtgctggt ggtgtacgac gacctcacca
     4681 cgcatgcgcg agcctatcgc gagctttcgc tgctgctgcg tcgcccgccc gggcgagaag
     4741 cctaccccgg cgatatcttc tacctgcacg cgcgcctgct cgaacgctcg acctgcctgg
     4801 cagcgcgcta cgggagcggc agcatgaccg ccctgccgat cgtcgaaacc gagcagggcg
     4861 agatcgccgc ctacattccc accaacctga tctcgatcac cgacggccag atctacctcg
     4921 acccggcgct ctacgcacgc ggttttctgc ccgccatcga catcacccgg tcggtttcgc
     4981 ggatcggcgg caaggcccag ttgccggcca tcaaggccga ggccgggcgg atgaagctcg
     5041 atttcctgca gtttctcgag ctggaagtat tcacccgctt
//
LOCUS       DAOSIT010000107         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14715_length_5075_cov_8.123506, whole genome shotgun sequence.
ACCESSION   DAOSIT010000107 DAOSIT010000000
VERSION     DAOSIT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14715_length_5075_cov_8.123506"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..579)
                     /locus_tag="PLL14_03865"
     CDS             complement(46..579)
                     /locus_tag="PLL14_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG03090.1"
                     /translation="MSNNETQTEAREEKSGKSASQRSALAPFKSFEDRFEQMEHFMNR
                     MMGNFLPSDWMRPFQRQLAGGNELAEFRAPAIDLIERDDEIVLRAELPGIKKEDVSIS
                     LTADSITIQGQSRKEEAEEKGDYRRREISSSAFSRSMWLPAGINSEAAKAVFNDGILE
                     VTLPKTPAAKRTTVPIE"
     gene            complement(608..829)
                     /locus_tag="PLL14_03870"
     CDS             complement(608..829)
                     /locus_tag="PLL14_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03091.1"
                     /translation="MDFLLADATGSGNSTQEIEFQKNSGRRVNFAVGQASGIHKKRWR
                     TRKNEIDLKDRLRDLYFLVKWVLAEVGER"
     gene            828..1292
                     /locus_tag="PLL14_03875"
     CDS             828..1292
                     /locus_tag="PLL14_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03092.1"
                     /translation="MSSNRVNIHFTAEQREEAKAALAALAAGLPFLIDLTSEERAAMP
                     RFGDKSRGFISKALDIAENNQEIFPRTFSLDEFRTRAAMLVGEGVLLRRRRMVLPAAR
                     ALFSRRERPSSNQDRGFPKPASVVPQRGTPLPAAARPFVVAATTIPGDRPRL"
     gene            1498..>5075
                     /locus_tag="PLL14_03880"
     CDS             1498..>5075
                     /locus_tag="PLL14_03880"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOG03093.1"
                     /translation="MTEHEIEQALIEKLGDLKYTYRPDIRDRAALEANFRQHFETLNR
                     VHLTRSEFARLLDGIVTPDVFAAARHLRERNSFERDDGTPLYYTLVNLKDWCKNSFEV
                     INQLRISTDYSHHRYDVILLINGIPAVQIELKTLAISPRRAMQQIVDYKSDPGNGYGK
                     SLLCFLQLFIVSNRSDTWYFANNNSRHFSFNADERFLPLYRFADVDNTRITHLDRFAD
                     TFLAKCTLGEMISRYMVLVASEQKLLMMRPYQIYAVKAIVDCIHQHCGNGYIWHTTGS
                     GKTLTSFKAATLLKDNPDIDKCLFVVDRKDLDRQTREEFNKFQEGCVEENTNTETLVR
                     RLLSDDYADKVIVTTIQKLGLALDGASANNAKRNYKERLEPLRHQRMVFIFDECHRSQ
                     FGENHQAIKEFFPNAQLFGFTGTPIFEQNASTQQIEGRQASYKTTADIFQQQLHAYTI
                     THAIEDRNVLRFHVDYYKVAGASSSGACSKPELEAPATLWEYLNPSTEIEIRSGENLP
                     HWEQGSVWYFVTFRLADALPLAVVEKLKKERERWKQTHDLDNLSSAELAEYHRLFSDR
                     YETLLNAGSGSCVLRNEVNAKIVRDALQYFEGQRYVLDEFVIMPNHVHVLVKPMNGHG
                     LPDITHSWKSFTANQINQRLGKTGSFWQHESYDHIVRHERAMEAIRRYIRENPTVAGV
                     SSSRFKTTLELEAPATIAKRAIVEAILARHDAATAGRKFNALLATASINDAIEYHALF
                     KTVQAERLHNRQAADPDFQPLNIACVFSPPADGNKDVQQIQEDLPQEKADNEQEPDKK
                     KAALKAIIADYNAQYGSNHGIGEFDGYYQDVQKRIKDQQYPNADLPHAQKIDITIVVD
                     MLLTGFDSKYLNTLYVDKNLKYHGLIQAFSRTNRVLNDTKPYGNILDFRQQQDAVDAA
                     IGLFSGQAARPAKEIWLVEPAAEVIRQYEAAVARMAGFMANKHLVCEPEQVYNLQGDS
                     ARIEFINRFKEVQRLKTQLDQYTELEPEQKARIEAALPKDQLLSFRSTYLETAQRLKA
                     QQDKGGDKTDPAVQQLDFEFVLFASALIDYDYIMALITRYASETPGKRKMSREQLIGL
                     IQSDAKFIDARDDIADYIDTLEAGKGLDEAAIRAGFDRFKADKHARQLADLAGQHGLE
                     AAALQGFVDGILRRMIFDGEALSDLMAPLALGWKARTKAELALMEDL"
BASE COUNT         1160 a         1692 c         1308 g          915 t
ORIGIN      
        1 ccgccactcg gttaccgcca ctcgcggggt cgcgagccca tcaagctatt cgattggcac
       61 cgtcgtccgc ttggccgcgg gggtcttggg aagcgtcact tcaaggatgc cgtcgttgaa
      121 caccgctttc gccgcttcgg aattgatccc ggcgggtagc cacatcgagc gggagaacgc
      181 gctcgaactg atttcgcgtc gccgataatc acccttttcc tcggcttcct ccttccggct
      241 ctgaccctgg atggtgatcg agtccgcggt cagcgagatc gaaacatctt ccttcttgat
      301 gccgggcaac tctgcccgca gcacgatctc gtcgtcgcgc tcgatcaggt cgatcgctgg
      361 cgcgcgaaat tccgccaact catttcctcc cgccaattga cgctgaaagg ggcgcatcca
      421 gtcactcggc aggaagttgc ccatcatccg attcatgaaa tgctccatct gttcgaagcg
      481 atcctcgaag ctcttgaaag gagcaagcgc gctgcgctga ctcgcagact tgccgctctt
      541 ctcctctctt gcttccgttt gggtttcatt gttcgacatg atcatctctc ctctgcagaa
      601 gttaccgtca ccgctcgccg acttcagcga gcacccactt cactaaaaaa tataggtcgc
      661 gcagtcggtc tttcaaatca atttcatttt tccgggtcct ccatcgtttc ttatggattc
      721 ccgaggcctg gcccacggcg aagttgacac gccggccgga atttttttga aattcaattt
      781 cctgagtgct gtttcccgac cccgttgcat cagcaaggag aaagtccatg tccagcaatc
      841 gcgtcaacat ccacttcacc gccgaacagc gcgaagaggc gaaagccgcc ctggctgctc
      901 tcgccgccgg gctgcctttc ctgatcgacc tgaccagtga agaacgtgcg gcgatgccgc
      961 gttttggcga caagagccgc ggcttcatca gcaaggcact cgacatcgcc gaaaacaacc
     1021 aggagatctt cccgcgcact ttcagtcttg acgaattccg cacacgagcg gcgatgctcg
     1081 ttggtgaagg cgtactcctt cgccgacgaa gaatggtcct gccggcggca agggcactgt
     1141 tttcgcggcg agaacgaccc tcttcgaatc aggaccgcgg ctttccgaaa cccgcaagcg
     1201 tcgtgccgca gcgaggaacc ccgcttcctg cagcagcaag acctttcgtg gttgcggcga
     1261 cgacgatccc tggtgatcgt ccaaggcttt agttcgcgag gcacggttca aagcggttcg
     1321 tcccggtgcg cagccacgct tcgaaggtga tgtgagcatc tcgccaccgc cgcgccatcg
     1381 ccgactgaac gcgcccgtct gtccgaaatt cccgagttta ccggttcagt taatagataa
     1441 tcgctgtcct catcgatcac aaatgagcac catttcaaaa agtacagaca attttcaatg
     1501 actgagcacg aaatcgaaca agccctgatc gagaaactgg gcgacctcaa atatacctac
     1561 cgcccggaca ttcgcgaccg cgccgcgctg gaagccaact tccgccagca cttcgagacg
     1621 ctcaaccgcg tccacctcac cagatccgaa ttcgcccgcc tgctggatgg catcgtcacc
     1681 cccgacgtgt tcgccgccgc cagacacctg cgcgagcgca acagcttcga gcgcgacgac
     1741 ggtacgccgc tctactacac cttggtcaac ctcaaggact ggtgcaagaa cagcttcgag
     1801 gtgatcaatc agttacgcat cagcaccgac tacagtcacc accgatacga cgtgatcctg
     1861 ctcatcaacg gcatccccgc cgtccagatc gagctgaaaa cgctggccat cagcccgcgc
     1921 cgggccatgc agcagatcgt cgactacaag agcgaccccg gcaatggcta cggcaagtcc
     1981 ctgctctgct ttctgcaact gttcatcgtc agcaaccgca gcgacacctg gtacttcgcc
     2041 aacaacaaca gccgccactt cagcttcaac gccgacgaac gtttcctgcc gctctatcgg
     2101 ttcgcggacg tggacaacac caggatcacg catctggacc gcttcgccga caccttcctc
     2161 gccaagtgca cgctgggcga gatgatcagc cgctacatgg tgctggtcgc cagcgagcaa
     2221 aagctgctta tgatgcggcc gtatcagatc tatgccgtca aggcgatcgt cgattgcatc
     2281 caccagcact gcggcaacgg ctacatctgg cacaccaccg gcagcggcaa gaccctcacc
     2341 tcgttcaagg ccgccaccct gctgaaagac aatcccgaca tcgacaaatg cctgttcgtg
     2401 gtggaccgca aggacctcga ccggcagacg cgcgaggaat tcaacaagtt tcaggaaggc
     2461 tgcgtcgagg agaacaccaa caccgaaacc ctggtgcgcc gcctgctctc cgacgattac
     2521 gccgacaagg tcatcgtcac caccatccag aagctcggcc tggcgctgga tggcgcatcc
     2581 gccaacaacg ccaaacgcaa ctacaaggaa cggctggagc cgctgcgcca ccagcgcatg
     2641 gtcttcatct tcgacgaatg ccatcgctcg caatttggcg agaaccacca ggcgatcaag
     2701 gaattcttcc ccaacgccca gttgttcggt tttaccggca cgccgatctt cgagcagaac
     2761 gccagcaccc agcagatcga aggccggcag gcgagctaca aaaccacggc cgacatcttc
     2821 cagcagcaac tgcacgccta caccatcacc cacgccatcg aagaccgcaa cgtgctgcgc
     2881 ttccatgtcg attactacaa agtggccgga gcttccagct ccggagcttg ctcaaaaccg
     2941 gagctggaag ctccggctac tttatgggaa tacctcaacc catcgaccga aattgaaatc
     3001 cgctccggcg agaatcttcc ccactgggag cagggctcgg tttggtattt cgtaaccttt
     3061 cgattggccg atgcactccc cctggcggtt gtcgaaaagc tcaaaaaaga acgcgaacgc
     3121 tggaaacaaa cccacgacct tgacaacctc agctccgcag aactcgccga gtatcaccgc
     3181 cttttctccg accgctacga aaccctgctg aatgcgggca gtggctcatg tgtcctgcgt
     3241 aatgaagtta acgccaaaat tgttcgtgat gcgcttcagt actttgaagg acagcggtat
     3301 gtcctggacg agtttgtcat catgcccaac cacgtccatg ttttggtcaa gcccatgaat
     3361 ggtcatggat tgcctgatat cacgcattcc tggaaatcat tcaccgccaa tcaaatcaac
     3421 cagcgcctgg gaaaaacggg gtcgttttgg cagcacgagt cctacgacca catcgtgcgc
     3481 cacgagcgtg caatggaggc cattcgtcgc tacatccgtg agaacccaac agtagctgga
     3541 gtttccagct cccgtttcaa aacaacactg gagctggaag ctccagctac tatagccaag
     3601 cgcgccatcg tcgaagccat cctcgccagg cacgacgccg ccaccgccgg gcgcaaattc
     3661 aacgccttgc tagccacggc aagcatcaac gacgccatcg aataccacgc cctgttcaag
     3721 accgtgcagg cagaaagatt gcacaatcga caggccgccg accccgactt ccagccgctc
     3781 aacatcgcct gcgtcttctc cccgcctgcc gacggcaaca aggacgtgca gcagattcag
     3841 gaagacctgc cgcaggaaaa agccgacaac gaacaggaac ccgacaagaa gaaggccgct
     3901 ctcaaggcca tcatcgccga ctacaacgcc cagtacggca gcaaccacgg catcggcgaa
     3961 ttcgacggct actatcagga cgtgcagaag cgcatcaagg accagcaata ccccaacgcc
     4021 gacctgccgc acgcgcagaa gatcgacatc acgattgtgg tggacatgct gctcaccggc
     4081 ttcgattcca aatacctgaa caccctctac gtcgacaaga acctcaaata ccacggcctg
     4141 atccaggcgt tctcgcgcac caaccgcgta ctcaacgaca ccaagcccta cggcaacatc
     4201 ctcgatttcc gccagcagca agatgcggtc gacgccgcca tcggcctctt ctccggccag
     4261 gccgccaggc ccgccaagga aatctggctg gtcgagccgg ccgcagaagt catccgccag
     4321 tacgaggcgg cggtcgcgcg catggcggga ttcatggcaa acaagcatct ggtctgcgaa
     4381 cccgagcagg tctacaacct gcagggcgac agcgcccgca tcgagttcat caaccgcttc
     4441 aaggaagtgc agcgcctgaa gacgcagctc gaccagtaca ccgaactcga acccgagcag
     4501 aaggccagga tcgaggcggc gctgccgaaa gaccagttgc tgagcttccg cagcacctat
     4561 ctggaaaccg cccagcgcct gaaagcgcaa caggacaagg gcggcgacaa aaccgacccg
     4621 gccgtgcagc agctcgattt cgagttcgtg ctgttcgcca gcgccctcat cgattacgac
     4681 tacatcatgg cgctcatcac ccgctacgcg agcgaaacgc cgggcaaacg gaagatgagc
     4741 cgcgagcaac tcatcggcct catccagtcc gacgccaagt tcatcgacgc gcgcgacgac
     4801 atcgccgact acatcgatac cctggaagcc ggcaaggggc tggacgaagc cgccatccgc
     4861 gccggcttcg atcgcttcaa ggccgacaag cacgcccgcc agttggcgga cctcgccggc
     4921 cagcacgggc tggaggccgc cgccctgcaa ggctttgtgg acggcatcct gcgccggatg
     4981 atcttcgatg gcgaagcctt gagcgacctc atggcgccgc tggcactggg ctggaaagcc
     5041 cgcaccaagg cggaactcgc gctgatggaa gacct
//
LOCUS       DAOSIT010000108         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14762_length_5063_cov_9.803115, whole genome shotgun sequence.
ACCESSION   DAOSIT010000108 DAOSIT010000000
VERSION     DAOSIT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14762_length_5063_cov_9.803115"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="rlmH"
                     /locus_tag="PLL14_03885"
     CDS             complement(<1..458)
                     /gene="rlmH"
                     /locus_tag="PLL14_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG03094.1"
                     /translation="MKLAILAVGHKPPAWVAQGCAEYQKRMPRELPLSIVEVKPESRG
                     SKNREQLLAAEKVRLVAALQGYARMVVLDERGADLPTRKLAERLECWMRAGGDTAFVI
                     GGADGIDAALKLQADDAIRLSSLTLPHALARLILCEQLYRAISIVRQHPY"
     gene            complement(455..838)
                     /gene="rsfS"
                     /locus_tag="PLL14_03890"
     CDS             complement(455..838)
                     /gene="rsfS"
                     /locus_tag="PLL14_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG03095.1"
                     /translation="MKIPQLEKLVVDALEDIKGHDIEVINTSRLTSLFDRMVIACGES
                     NRQVKSLARNVQDKVRAAGAPVLSCEGEEVGEWILVDLGDIIVHVMQPAVRSYYNLEE
                     LWGGKGPVRVRRTPAVAQDATAGQG"
     gene            complement(846..1520)
                     /gene="nadD"
                     /locus_tag="PLL14_03895"
     CDS             complement(846..1520)
                     /gene="nadD"
                     /locus_tag="PLL14_03895"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOG03096.1"
                     /translation="MPEQPGERPLGIFGGTFDPVHFGHLRLAEEAADSLDLAGIRWIP
                     AGQPALREVPRVTAGQRLEMVRLATAGNPRFSVDAAEVEAARPSYSVQTLERLRQASA
                     CGRRRPLVLLLGADAFIGLPKWHRWQALFDLAHLAVAHRPGFAIDAGQLPTPLAPCYR
                     DRFCASPALLAESPAGRIVTFAMTQLAISATRIRALLASGGSARYLLPDAVLTYIRQH
                     GLYPEY"
     gene            complement(1513..2289)
                     /locus_tag="PLL14_03900"
     CDS             complement(1513..2289)
                     /locus_tag="PLL14_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOG03097.1"
                     /translation="MQALTLPAAQGWRWLTDGFRIFRRNHLMLTFLVVSYWVLMALVN
                     VIPVIGTIATTLCIPAFSVSLMNACRSIERGTPLGPQLLFSGFASNLRALLTLGAIYL
                     AAVVAILGLSALADDGALMAMMLAGQKPDAETVSSGSLLLATEIALVLLCPLIMAYWY
                     APVLAGWHGLSPAKALFFSFVACLRNWRPFLVYSLAIGVVATLVPGIVLGILAALLPS
                     GASLVLVLMMVLLILVLAPTLFASFYVSYRDVFVSADDDA"
     gene            complement(2308..3045)
                     /locus_tag="PLL14_03905"
     CDS             complement(2308..3045)
                     /locus_tag="PLL14_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOG03098.1"
                     /translation="METFPIPAPGFNGASRRTEVDNVFNWFRQGWAIFIVNPGAWIAM
                     MVILIVIYLGLAIVPWIGPLASYLLTPVLAAGMLLACERAARQEEFAITDLFAGFQHH
                     TGSLVMLGVLYMLGMLVVFVLAFVFAGGGVAGGLLIGNPIGAGVALGGVFLAGLLWML
                     LSLPIVMAIWFAPALVVFNRLQPVDALKASFHACLKNTLVFLVYGLISLVLCFFAALP
                     LGLGFLVLGPVLAGSVYASYRDIFLGA"
     gene            complement(3077..>3276)
                     /locus_tag="PLL14_03910"
     CDS             complement(3077..>3276)
                     /locus_tag="PLL14_03910"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765686.1"
                     /note="catalyzes the formation of O-phospho-L-homoserine
                     from L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOG03099.1"
                     /translation="ERRAWPTMLRAAALRFWISRLQDHHLPRPGSLVIRRDPDAYRAI
                     LLTHIASDASQVRLATSQPCG"
     assembly_gap    3277..3376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3377..3759)
                     /locus_tag="PLL14_03915"
     CDS             complement(<3377..3759)
                     /locus_tag="PLL14_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOG03100.1"
                     /translation="MSVFTELSTAQASAWLEDYALGSLVTLAGIAEGVQNSNFFLDTT
                     LGQYVLTIFEQQPGAQLPFHLQLLAHLSARGIPCPAPVTNRRHEYLGEIAGKPAAIVS
                     RLPGSSRSVPAVGHCAAIGSMLARL"
     gene            complement(3813..4172)
                     /locus_tag="PLL14_03920"
     CDS             complement(3813..4172)
                     /locus_tag="PLL14_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HOG03101.1"
                     /translation="MRRLQALRSALVLLVLLVLAAAPAVGQNRTDLQPLPAVPPPPPE
                     MAPFDASLEPEVTIKKREGDTVEEHRINGKLYRIKVTPEHGVPYYLTDRQGDGNFTQE
                     TMGTPDVSVPTWVIGTF"
     gene            4387..>5063
                     /locus_tag="PLL14_03925"
     CDS             4387..>5063
                     /locus_tag="PLL14_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOG03102.1"
                     /translation="MPTLLLVDGSSYLYRAFHAMPDLRTSQNEPTGAIHGVLNMLRRL
                     ESDYQASGIDYKGCVFDAKGRTFRDDWYPQYKANRPPMPEDMVRQIEPLHAAIVALGW
                     PLLMCDGVEADDVIGTLTTQAAAQGVQVVVSTGDKDLAQLVGPHVSLINTMSNEVLDE
                     AGVLGKFGVPAERIVDYLALIGDAVDNVPGVDKVGAKTAVKWLAQYGSLDGVIAAAAD
                     IGGVVGQ"
BASE COUNT          907 a         1565 c         1744 g          747 t
ORIGIN      
        1 tggtaagggt gctggcggac gatgctgatc gcgcgataca actgctcgca gaggatcagc
       61 cgcgccaggg cgtgcggcag ggtcaggctg gagaggcgaa tcgcgtcgtc ggcctgcagc
      121 ttcagagccg cgtcgatgcc gtcggcaccg ccgatgacga acgcggtatc gccgccggcg
      181 cgcatccagc attcgaggcg ctcggcaagt ttccgggtcg gcaggtcggc gccgcgttcg
      241 tcgagcacca ccatccgcgc atacccttgc aacgcggcca cgaggcgcac cttctcggcg
      301 gccagcaact gctcgcggtt cttcgagccg cggctttccg gtttgacttc gacgatcgac
      361 aggggcagtt cgcggggcat gcgcttctgg tactcggcgc agccctgcgc cacccaggcg
      421 ggcggcttgt gaccgacggc gagtatggcc agcttcatcc ctggcccgct gtcgcatcct
      481 gggccacagc gggggtccgg cgcacgcgca cgggtccctt gccgccccac aactcttcga
      541 gattgtaata actgcgtacc gccggctgca tgacatgcac gatgatgtcg ccgaggtcga
      601 ccaggatcca ttcgccgacc tcctcgcctt cgcacgagag gaccggcgca ccggcggcgc
      661 gtaccttgtc ctgaacgttg cgggccagcg acttgacctg ccggttcgat tcgccgcagg
      721 cgatgaccat gcggtcgaaa agcgaggtca ggcggctggt gttgatcacc tcgatgtcgt
      781 ggcccttgat gtcttcgagg gcgtcgacga cgagcttttc cagttgggga atcttcattg
      841 tggcttcagt actccgggta aaggccatgc tggcgaatat aggtaagcac cgcgtcgggc
      901 agcaggtagc gcgcactgcc gccactggcc aggagggcgc gaatccgtgt cgccgagatg
      961 gcgagttgcg tcatcgcaaa ggtgacgatg cgtcccgccg gcgactcggc gagcagcgcc
     1021 ggactggcgc agaaacggtc gcggtagcag ggcgccagcg gcgtgggcaa ctgcccggcg
     1081 tcgatggcga agccggggcg atgggcgacg gcaaggtgcg ccagatcgaa cagcgcctgc
     1141 cagcggtgcc acttcggcag gccaatgaag gcatcggcac cgagcagcag caccaacggg
     1201 cgccgccgtc cgcacgcgct ggcctggcgc agacgttcga gcgtctggac gctgtagctt
     1261 ggccgggccg cttcgacttc ggccgcatcg acgctgaagc gcgggttgcc ggcagtggcc
     1321 aggcgcacca tctccaggcg ttgcccggcg gttacccgcg ggacctcgcg cagcgccggc
     1381 tggccggccg gaatccagcg gatgccggcc agatcgaggc tgtcggcagc ctcctcggcc
     1441 aggcgcaagt gaccgaaatg aaccgggtcg aaggtgccgc cgaagattcc cagcggccgt
     1501 tcgccgggtt gctcaggcat cgtcgtccgc gctgacgaag acgtcgcgat aactgacgta
     1561 gaagctggcg aacagcgtcg gggcgagcac caggatcagc agcaccatca tcaacaccag
     1621 caccagcgag gcaccgctgg gtagcagggc cgccagaatg ccgagcacga tgcccggtac
     1681 cagcgtggcc acgacgccga tggccagcga gtacaccagg aacgggcgcc aattgcgcag
     1741 gcaggcgacg aaactgaaga acagtgcctt ggcgggcgac aggccgtgcc atccggcaag
     1801 aaccggcgcg taccagtagg ccatgatcag cgggcacagc aggaccagcg cgatctcggt
     1861 cgccagcagc aggctgccgc tgctgacggt ttcggcgtcc ggcttctggc cggccagcat
     1921 catcgccatc agggcaccgt cgtcggccag cgccgagagg ccaagaatcg ctaccaccgc
     1981 ggccagatag atcgctccga gggtgagcag cgcgcgcagg ttgctggcaa agccggaaaa
     2041 cagcagttgc ggacccagtg gcgtgccgcg ctcgatgctg cggcaggcat tcatcaggct
     2101 caccgagaaa gcggggatgc acagcgtcgt ggcgatcgtg ccgatcacgg gaatgacatt
     2161 caccagggcc atcaacaccc agtagctgac caccaggaag gtcagcatca ggtgattcct
     2221 gcggaagatc cggaagccgt cggtcagcca gcgccagcct tgtgcggcgg gaagagtcag
     2281 tgcttgcatg gtttgtcgta gggtcgctca ggcgccaagg aagatgtcac gataggaagc
     2341 atacaccgag ccggcgagca ccggtccgag gaccaggaac ccgagaccca gcggcagcgc
     2401 ggcgaagaag cacagcacca ggctgatcag gccgtagacc aggaacacga gggtgttctt
     2461 gaggcaggca tggaagctcg ccttgagggc atcgaccggc tgcagccggt tgaagaccac
     2521 cagcgccggc gcgaaccaga tggccatgac gatcggtagc gacagcagca tccacagcag
     2581 cccggccagg aagacgccgc cgagggcgac accggcaccg atcgggttgc cgatcagcag
     2641 gccgccggcg acgccaccgc cggcgaagac gaaagccagc acgaagacga ccagcatgcc
     2701 cagcatgtac agcacgccga gcatcaccag gctgccggtg tggtgctgga aaccggcgaa
     2761 aaggtcggtg atggcgaact cctcctggcg ggcggccctc tcgcaggcga gcaacattcc
     2821 ggccgcgagg accggggtca gcagatacga agcgagtgga ccgatccagg ggacgatcgc
     2881 caggcccagg tagatgacga tcaggatcac catcatcgct atccaggcgc cgggattgac
     2941 gatgaagatc gcccagccct gccggaacca gttgaagacg ttgtcgacct cggttcggcg
     3001 gctggcgccg ttgaagccgg gggcggggat cgggaaggtt tccatgcgcg tgctcgtgtc
     3061 tgggcgaggt ggccgatcag ccgcagggct gggaggtggc aagccgcacc tggctggcgt
     3121 cgctggcgat gtgggtcagc aggatggccc ggtaggcgtc ggggtcgcgc cggatgacca
     3181 ggctgccggg ccgcggcaga tggtggtcct gcagccggga gatccagaaa cgcagtgccg
     3241 ccgcacgcag catcgtcggc caggcgcgcc gctcggnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnntgca gacgcgccag catgctgccg atcgccgcgc agtggccgac
     3421 cgccgggacg ctccgggaac ttccgggcag ccggctgacg atcgccgcgg gcttgccggc
     3481 gatctcgccg aggtattcgt gacgacggtt ggtgaccggt gccgggcagg gaattccccg
     3541 cgcggacagg tgcgccagca gttgcaggtg gaagggaagc tgcgcgccgg gttgctgctc
     3601 gaaaatcgtc agcacgtact ggcccagcgt cgtgtccaga aagaaattgg agttctgcac
     3661 gccctcggca ataccggcga gcgtcaccag gctgcccagc gcatagtcct ccagccaggc
     3721 gctcgcctgc gcggtgctca gttcggtgaa caccgacatg gcgacccggc gcgaccggcc
     3781 ggcaggcgag agggaatccg caaggggtgg gttcagaaag tgccgatgac ccaggtcggc
     3841 acgctgacgt cgggtgtgcc catcgtttcc tgggtgaagt tgccgtcgcc ctgtcggtcg
     3901 gtcaggtagt agggaacgcc gtgttcgggg gtgaccttga tgcggtacag cttgccgttg
     3961 atgcggtgct cctcgacggt atcgccttcg cgcttcttga tcgtcacctc gggttccagc
     4021 gatgcatcga agggcgccat ctccggcggt ggcggcggca ccgccggcaa gggctgcaga
     4081 tccgtccggt tctggccaac ggcaggcgca gcagcgagga ccaggaggac caagagtacg
     4141 agggcggagc ggagggcttg aaggcggcgc atgagcagtt tcccgggtga ttggtcgatc
     4201 agtgagcatc tcacctgtca gtgtggattg tagtacagat tgaggaactg cccttgcccg
     4261 aaggcgcgac ggcgacgcgg tggcctggcg aactctgcga taatagacgc cgggactcga
     4321 accacccgcc gctgtcgccg tccgacagga gagtcctccc ctcggtcaaa actgtgttgc
     4381 ttgcctatgc ccaccctgct gctggtcgac ggatcgtctt acctctaccg cgccttccat
     4441 gccatgcccg acctgcggac ctcgcagaac gagcccaccg gagcgatcca cggcgtgctg
     4501 aacatgctgc ggcgcctgga aagcgattac caggcgtcgg gaatcgacta caagggctgc
     4561 gtcttcgacg ccaaggggcg gacctttcgt gacgactggt acccgcagta caaggccaat
     4621 cgcccgccga tgcccgagga catggtgcgt cagatcgaac ccctgcacgc ggcgatcgtt
     4681 gccctcggct ggccgctact gatgtgcgac ggcgtcgagg cggacgacgt gatcggtacg
     4741 ctgaccacgc aggcggcggc gcagggcgtg caggtggtgg tgtcgaccgg cgacaaggat
     4801 ctcgcgcaac tggtcggccc gcacgtcagc ctgatcaaca cgatgagcaa cgaggtgctc
     4861 gacgaagccg gcgtgctcgg caagttcggc gtgccggcgg agcgtatcgt cgattacctg
     4921 gcgctgatcg gcgacgccgt cgacaacgtt ccgggcgtcg acaaggtcgg cgccaagacc
     4981 gccgtcaagt ggctcgcgca gtatggttcg ctcgacgggg tgatcgctgc cgccgccgac
     5041 atcggtggcg tggtcgggca gaa
//
LOCUS       DAOSIT010000109         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14771_length_5060_cov_8.722478, whole genome shotgun sequence.
ACCESSION   DAOSIT010000109 DAOSIT010000000
VERSION     DAOSIT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14771_length_5060_cov_8.722478"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1439)
                     /gene="prsR"
                     /locus_tag="PLL14_03930"
     CDS             complement(81..1439)
                     /gene="prsR"
                     /locus_tag="PLL14_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOG03103.1"
                     /translation="MSSEKLPHLLIVEDDRALQKQIRWAFDQYETLTADDRESALVLI
                     RRYQPPVVTMDLGLPPDADSVSEGFRLLEEILTLAPDTKVIVLTGQNDRANALRAIAL
                     GAYDFFAKPFEIDMLGLTIQRAYRLYELQKENERLQSMQHPDVLAGLITRDPELLRVC
                     RTIERVAVTDATVLLLGESGTGKELLARGVHESSPRRRERFVAINCAAIPENLLESEL
                     FGYEKGAFTGAARTTPGKIETANNGTLMLDEIGDLPHSLQAKLLRFLQERVIERIGGR
                     QEIPVNVRIVCATHQNLKTLISEGRFREDLFYRLAEIVVNIPPLRARSGDANLLAHAF
                     LRRFAAENNRGGMTLREDALRTIEAYPWPGNVRELENCLKRAVIMADGNQITADDIGL
                     AGAAPADEAVLDLRHARDDAERRVVITALARADGNVVKAAELLGVSRPTLYDMMHRFG
                     LK"
     gene            complement(1436..3547)
                     /gene="prsK"
                     /locus_tag="PLL14_03935"
     CDS             complement(1436..3547)
                     /gene="prsK"
                     /locus_tag="PLL14_03935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HOG03104.1"
                     /translation="MDSKMAMVAAWSYGLAGVFASFLTLYLASGWRAGGRSRAMFLAV
                     ALCALWGMLGLAFALTGQAVFLAGSLLADAPRFGGWYFFLLILMKPEATEGERPTPRP
                     GWLGAIALLLCLVGFAAQLLAVLGIDLVLPSQRVVLFASLAMSVFGLVLIEQLYRTVS
                     PDFRWSIKPLCLGLGGVFLFDLYLYSDALLFNRVDVDAFSIRGFAHALGLPLVALSAI
                     RSHDWKRRLVMSQRAALQSATLVIVGAYLLFMASAGYYVRFFGGEWGRALQLALLFAA
                     LLVLAGLALSGSMRARLRVQVGKHFFSYRYDYREEWLRFTQTLSSQGGFTGMGRHVVR
                     GLADMVESPSGALWLKDPSGRFFAQAACWNMPASVVTEDADSPLCKFLLESGWVINLE
                     EYRSLPRRYDGLKLPAWVVEVPNAWLVVPLTTGSELIAFVVLATARTRIDVNWEVNDL
                     LKTAARQAGAFLGQMQASEALLEVRKFDSFNRMSAFVVHDLKNIIAQLSLMLKNAERH
                     RDNPEFQKDMLMTVEHSVERMRQLMMQLREGATPLDGPRGIDLVDVLRRIQSAKAGQG
                     REVALTVDERVVARGHEDRIERVIGHLVQNALDATEHGGRVWIRVARQGTHALVEVGD
                     TGHGMSPEFVRERLFKPFQTTKPTGMGIGAYESFQYVHELGGKLSVDSAVDVGTQVAL
                     LLPLFDTGHGMKSTETPKESA"
     gene            complement(3609..4991)
                     /locus_tag="PLL14_03940"
     CDS             complement(3609..4991)
                     /locus_tag="PLL14_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOG03105.1"
                     /translation="MIKVFNHWFHRKTVAQVAVDLMFPVACVLFAAAWIGHSGHLAVE
                     KVAFYAVIFAVTTIVFNAWLGIYQRVHSRTRTETRARAVLSLYLAIPLAYVVFSLLSV
                     EEVDQGFLLLSGMAALFATLVRRVHSAHSRSGSLMRHRVLVFGVGDEAENVGRVLRKS
                     DPDIEIVGFYPCATDTEIVVPSQVVLSRQMSLPDTAHALKVDEIIVAVRERRGGALPL
                     RELLDCKLSGVRVLDLASYFERALGQLRLDSLRVGWLIFGEGFRQSWRRTTIKRFFDV
                     VVASMLLVLAMPVMLVTALLIILEDGFPVFYRQERVGLEGRLFKVIKFRSMRNNAESD
                     GKPRWATTNDDRVTRVGRVIRKLRIDELPQLCNVLAGDMSLVGPRPERPYFVDQLTRD
                     IPFYAVRHSVKPGLTGWAQVCYHYGSTVDDSVQKLQYDLYYVKNHTLFVDIVILFQTV
                     GVVLTGKGAR"
BASE COUNT         1108 a         1674 c         1427 g          851 t
ORIGIN      
        1 ctgccctgcg cagtttgtgc agcgagttgc gagtattcat aagggtcctc acggagtcga
       61 taaatcgtct tggacaggcg ctacttcagt ccaaacctat gcatcatatc gtacagagtc
      121 ggtcggctga cgccaagcaa ctcggcagcc ttgaccacgt tgccgtcggc gcgcgccagg
      181 gcggtgatga ccacccgccg ctcggcgtca tcgcgcgcat gtcgcaggtc cagcaccgcc
      241 tcgtctgccg gcgccgcgcc ggcaaggccg atgtcatcgg ccgtgatctg gttgccatcg
      301 gccatgatca ccgcccgttt gaggcagttc tccagttccc gaacgttgcc cggccaggga
      361 taggcttcga tcgtccgcaa ggcatcctcg cgcagggtca tcccgccgcg attgttctcg
      421 gcggcaaaac gccgcaagaa cgcatgcgcc aacaagttgg cgtcgcccga gcgcgcccgc
      481 aggggcggaa tgttcacgac aatctccgcc aaccgataaa acaggtcctc acgaaaacgt
      541 ccctcgctga tcagggtctt gagattctgg tgcgtcgcgc aaacgatgcg gacgtttacc
      601 gggatctcct gccgcccgcc gatcctctcg atgacccgct cctggaggaa acgcagcagc
      661 ttcgcctgca gcgaatgcgg caggtcgcca atttcgtcca gcatcagggt gccgttgttc
      721 gccgtttcga tcttgcccgg ggtggttctc gccgcaccgg taaaggcccc cttctcgtag
      781 ccgaagagtt cgctttccag caggttttcc ggaatggccg cacagttgat ggccacgaac
      841 cgctcgcggc gacgcggcga cgactcatgc accccacgcg ccagcagttc cttgcctgtc
      901 ccgctctcgc cgagcagcaa taccgtcgca tcggtcaccg caacccgctc gatggtacga
      961 cagacgcgca gcaactccgg gtctcgggta atcagcccgg ccagcacgtc gggatgctgc
     1021 atagactgca gccgttcgtt ttccttttgc agctcataga gtcggtaggc gcgctgaatc
     1081 gtcaggccca gcatgtcgat ttcgaacggt ttggcaaaaa aatcataggc gccgagggca
     1141 atcgcgcgca aagcgttggc ccggtcgttc tgtccggtca ggacaatgac cttggtatcc
     1201 ggcgccaggg tcaggatctc ttcgagcaac ctgaaacctt cggagaccga gtcggcgtcg
     1261 ggcggcaaac cgagatccat ggtgaccact ggcggctgat agcgccggat cagcaccaga
     1321 gcgctctctc gatcatcggc ggtcagcgtc tcgtactggt cgaaagccca gcgaatctgc
     1381 ttctgcagcg ctcgatcatc ctcgacgatg agcaggtgcg gcaacttctc ggaactcatg
     1441 ccgactcctt cggtgtctct gtggacttca tgccatggcc ggtatcgaat aagggcagca
     1501 gcagcgccac ttgcgtgcct acatcgactg cgctatcgac agacagcttg ccgcccagtt
     1561 cgtgaacata ctggaaactc tcgtaagcgc cgatacccat tcctgtcggc ttggtcgtct
     1621 ggaaaggttt gaacagccgc tcgcgaacaa actctggact catcccatgc ccggtatcgc
     1681 ccacctcgac cagagcatgc gtcccctgac gggccacccg gatccagaca cgcccgccgt
     1741 gctcggtggc atcaagcgca ttctgcacca gatgaccgat cactcgctca atccggtctt
     1801 cgtgcccgcg cgcgacgacc ctttcgtcga ccgtcaatgc aacctcccgt ccctgcccag
     1861 ccttggccga ctggatccgc cgcaggacgt cgaccagatc gattcccctg ggaccgtcaa
     1921 gcggtgttgc gccttcacgc aactgcatca tcagttgccg catccgctcg acagagtgtt
     1981 cgacggtcat caacatgtcc ttctggaact cggggttgtc gcgatgacgc tcggcgttct
     2041 tgagcatcag cgacaactgg gcaatgatgt tcttcaggtc atggaccaca aatgccgaca
     2101 tgcggttgaa cgaatcgaac ttccggacct caagcagggc ttcgctggct tgcatctggc
     2161 caagaaaggc tcctgcctgc cgtgccgccg tcttgagcag atcgttgacc tcccagttga
     2221 cgtcgattct tgtccgcgcc gtggccagca caacgaaagc gatcagttca ctgcccgtcg
     2281 tcagtggcac caccaaccag gcattcggca cctcgacgac ccaggccggg agtttgagac
     2341 catcgtagcg acgcggcagg gaacgatact cttccagatt gatcacccag ccactttcaa
     2401 gcaggaattt gcacaaaggg ctgtcggcat cctccgtgac cactgaagcg ggcatgttcc
     2461 agcaggccgc ctgcgcaaag aagcgaccgg agggatcctt gagccacagt gccccgctcg
     2521 gactctcgac catgtcggcc agaccccgca ccacgtgccg ccccatgccc gtgaagccgc
     2581 cttgcgagga aagtgtctgg gtaaaacgca gccactcctc gcggtagtcg taacggtagc
     2641 tgaagaaatg cttgccgacc tgtacccgca atctggcgcg catcgaaccc gacagggcca
     2701 gccccgcgag gaccagcaag gccgcaaaga gcagcgccag ttgcagcgct cttccccatt
     2761 cgccaccaaa aaaacggacg taatagccgg cggacgccat gaacagaaga taggcgccga
     2821 cgatcaccag ggtcgccgac tgcagcgcgg ctcgctgcga catgaccagc cttctcttcc
     2881 agtcatggct acggatcgcc gacagcgcga ccagcggcaa gcccagggca tgcgcaaaac
     2941 cgcgaatgct gaaggcgtcc acatcgaccc gattgaacag cagtgcgtcc gaatacaggt
     3001 acaggtcgaa caggaagacg cctccaagcc caagacacaa gggcttgatg ctccagcgaa
     3061 agtcgggaga aacggtgcgg tacaattgtt cgatcaacac caggccgaac acactcatcg
     3121 ccagcgaggc aaacaggacc acccgctgcg acggcagcac cagatcgatt cccagtacag
     3181 ccagcagctg cgcggcaaaa ccgaccagac agagcagcaa tgcgatcgcg cccagccagc
     3241 cgggacgtgg cgtcggacgc tcgccttcgg tcgcttccgg cttcatcagg atcagaagga
     3301 agaagtacca gccaccgaaa cgcggcgcat cggccaacag gctaccggca agaaacacgg
     3361 cctgcccggt cagcgcaaat gccaggccca gcatccccca cagggcgcac agggcaaccg
     3421 cgagaaacat ggcacgactg cgaccaccag cgcgccagcc ggaggccagg tacagcgtca
     3481 ggaaggacgc aaaaaccccc gccagcccat aactccaggc ggcaaccatc gccatcttgc
     3541 tgtccatcac ggtcggaaat cctcgcgcat cgccaccgga cggcgctcgt caacccgtag
     3601 cggactgctc accgggcacc cttgccagtc aagaccacac cgaccgtctg aaacagaatc
     3661 acgatatcca cgaacagggt atggttcttg acgtaataca ggtcgtactg cagcttttgc
     3721 accgaatcgt caaccgtcga gccgtagtga tagcacactt gcgcccagcc ggtgagccct
     3781 ggtttgacgc tgtggcgcac ggcgtagaag ggaatgtcgc gggtcaattg gtcgacaaaa
     3841 taggggcgct cagggcgcgg accgaccaga ctcatgtccc ctgccaagac attgcagagt
     3901 tgcggcaact cgtcgatacg cagcttgcgg atcacccggc cgacacgcgt cacccgatca
     3961 tcattggtcg tcgcccagcg cggcttgccg tcactctcgg cgttgttgcg catgctgcga
     4021 aacttgatga ccttgaacag gcgcccctca agacccaccc gctcctgccg gtagaatacc
     4081 gggaacccgt cttcgaggat gatcaggagg gcggtcacca gcatcaccgg catcgccagg
     4141 accaagagca tcgacgccac cacgacatca aaaaaccgct tgatggtggt acgccgccaa
     4201 ctctgccgga atccctcgcc gaagatcagc cagccgacac gcagggaatc gagacgaagc
     4261 tgaccgaggg cgcgttcgaa gtagctcgcc agatccagca ccctgacgcc ggaaagtttg
     4321 cagtcaagga gttcgcgcag tgggagagcg ccaccacgac gctcacgtac cgcgacgatg
     4381 atctcatcga ccttcaaggc atgcgcggtg tcaggtaacg acatctggcg cgacagcacc
     4441 acctgcgagg ggacaacgat ctcggtgtcg gtggcacagg gatagaaacc gacgatctcg
     4501 atatccgggt ccgacttgcg cagcacccgc ccgacatttt ccgcctcgtc gccgaccccg
     4561 aagaccagca cccgatgccg catcaaggag ccgctgcgac tgtgcgccga atgcacacgc
     4621 cgcaccagcg tcgcaaagag ggcggccatg ccggacagca gaagaaagcc ctgatcgacc
     4681 tcttccaccg aaagcaggga aaagacgacg taggccagcg gaatcgccag gtacagcgac
     4741 aacactgcgc gtgcgcgcgt ctcggtgcgg gtacggctat gcacgcgttg atagataccc
     4801 agccaggcgt tgaagacgat cgtcgtcacc gcgaagatca ccgcatagaa agccaccttc
     4861 tccacggcga ggtgacccga atgtccgatc caggccgcgg caaacagcac gcaagcgaca
     4921 ggaaacatca ggtctacggc cacctgggca acggtcttcc gatggaacca atgattgaat
     4981 accttgatca cgatagactc cttgcaagca cgaacctgaa aacttcccgt tgatgaccga
     5041 tgcttgaaga accgccctcc
//
LOCUS       DAOSIT010000110         5029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14878_length_5029_cov_11.375352, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000110 DAOSIT010000000
VERSION     DAOSIT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14878_length_5029_cov_11.375352"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PLL14_03945"
     CDS             <1..1214
                     /locus_tag="PLL14_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG03106.1"
                     /translation="FPSHAAIRHASRHLRRWMRPQARSVSLWFQPGRAELRYQPLGAV
                     GIIVPWNYPIFLAAAPLAAALAAGNRALVKMSELTPATAALFAERVGRYFADDELSVV
                     EGDVGVAQEFSRLPFDHLLFTGSTAVGRQVMRAASDNLTPVTLELGGKSPAIIGPGLS
                     TAQFAHAVERIIVGKSMNAGQTCIAPDYVLLPAGHEQAFIDVARRVVGDCYPDIERTT
                     DYSTIVDQRQYARLCAYLDEARAAGARVVDLAPGAVADPAARRLPPLALLDAGDELRV
                     MQEEIFGPLLPLRPYRDLDAAIAFINSRPRPLALYYFDRAPANIERVLNETVAGGVTI
                     NDTILHIAQDDLPFGGVGPSGMGCYHGFEGFQTFSARKAVFRQSRLSAIGLFKPPYGA
                     VFERLTRILLR"
     gene            1211..1714
                     /locus_tag="PLL14_03950"
     CDS             1211..1714
                     /locus_tag="PLL14_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03107.1"
                     /translation="MIATRRQFLKTGVAGSLLLGCAGWLNAAGARPLSDAEREMLGAL
                     TNALLDGALPTDLAARRRLIAVTIDGIAVEIAGLSLASQKEIGELFGLLVLAPGRRFL
                     AGVGRPWREAAAGDVVEFLQAWRSSRLSLLQSAYAALHDLTFGAWYARPDTWEAIGYP
                     GPPRGYF"
     gene            1714..3321
                     /locus_tag="PLL14_03955"
     CDS             1714..3321
                     /locus_tag="PLL14_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOG03108.1"
                     /translation="MQDPIKAGLAAGWKHIDASLLASDLSIETDVVIVGTGAGGGVTA
                     DILSDAGLRVVLVEEGPLRSSSDFRMREADAYPELYQESASRKTADKAINILQGRCVG
                     GSTTVNWTSSFRTPPEVFDYWQRQFGLRQLSVESMSSWFAVMEHKLWVRAWETPPNAN
                     NKALAAGAEKLGIPIGSIKRNVKGCWNLGYCGMGCPTNAKQSMLLTTIPAALQRGATL
                     CTRLRAERLRFTGSRVTALDCVALQADGIHPGRQRVHLRARHFVIAGGAIGSPALLLR
                     SGVPDPQRLLGKRTFLHPVVISSALMPERVDAYAGAPQSIYTDHFLHQAPIDGPLGFK
                     LETPPLHPVLYATTLQGFGEAHGKLMREFTQVNVLLALLRDGFHPESRGGQVRLGSDG
                     LPVLDYPLGDVIWEAARRALLAMAEIQFAAGARWVTPVHETVPGYASWAEARKGIAEL
                     PLKPLLCRVVSAHVMGGCGMADTPQRGVVDHRGRHFWLGNLSVHDGSVFPTSLGANPQ
                     LSIYGLVARNASLLAAELSGRPLPAIL"
     gene            3336..4262
                     /locus_tag="PLL14_03960"
     CDS             3336..4262
                     /locus_tag="PLL14_03960"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG03109.1"
                     /translation="MLSRALRRSLALEVALYVALAIYGCDASPAAAAGVALAGVLALR
                     ATLTAVTYAYAWACHSPAPPLSPGQAVRMVLSEYAACVFSFVLIFPFERWWMAPDRLQ
                     TGAEPTAGRPDARPPVLLVHGYSCSRAAWWWLRCRLEAAGWRVATISLEPIYGSIDEY
                     VEPLARRIAAVLAETGAERLLLVGHSMGGLVARAYLQRHGAARVARLVTLGTPHQGSR
                     IAHLGLGENARQMQPDSAWLQALASPAPVLDTRVIYSPHDNFVMPQERLQLPGATSQA
                     IAGVGHLAMLYSPRVARALLAALAGTSNTGAA"
     gene            4270..4962
                     /locus_tag="PLL14_03965"
     CDS             4270..4962
                     /locus_tag="PLL14_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03110.1"
                     /translation="MRARVLLLPLSLLSALPSAAPAQDVFVTRGAGSPVFSDKPQAGA
                     KPLSLPPLNVIEPLPVSRAPAAATPAREESRRAEPAIPAYQKFSIVSPANDGSVLANT
                     ALFEVRVVVEPPLQLGEGHAVTVSINGRPVGQRFTAGEFMIPPEFWGDTLPPANQRHQ
                     LDAAIVDRNGAVLRRAEPVTFHLRYVAGLQHRHAPRSAPMPIPVDKPRPPAQPEPLIR
                     KFVQPARPLFEH"
BASE COUNT          717 a         1805 c         1694 g          813 t
ORIGIN      
        1 tgttcccgag ccacgcggcc attcgccatg ccagccgcca cctgcggcgc tggatgcggc
       61 cacaggcgcg ttcggtctcg ttgtggttcc agccgggccg ggccgaatta cgctatcaac
      121 ccctcggcgc cgtaggcatc atcgtgccct ggaattaccc gatcttcctg gccgcggcac
      181 cgctggcggc agccctggcg gcgggcaacc gcgcgctggt caagatgtcc gagttgactc
      241 ccgcaacggc ggcgctgttc gccgaacggg tcggccggta cttcgccgac gacgagctgt
      301 cggtggtcga aggcgatgtc ggtgtcgcgc aggaattttc gcggctgcct ttcgatcacc
      361 tgttgtttac cggctcgacg gcggtcggcc gccaggtcat gcgcgccgcc tccgacaatc
      421 tgacgccggt gacgctcgaa ctcggcggca agtcgccggc gatcatcggg cccggattgt
      481 cgacggccca gttcgcgcat gccgtcgagc gcatcatcgt cggcaagagc atgaacgccg
      541 gccagacctg cattgccccc gactacgtgc tgctcccggc cggacacgag caggctttca
      601 tcgacgtcgc ccggcgcgtc gtcggcgact gctatccgga catcgagcgg accaccgact
      661 actcgacgat cgtcgatcag cgccagtacg cccgcctctg cgcctacctc gacgaagcgc
      721 gggcggccgg agcgcgggtc gtcgatctcg cgccgggggc cgtcgccgac ccggcggcgc
      781 gtcgcctgcc gccgctggcg ctactcgacg ctggcgacga actgcgcgtg atgcaagagg
      841 aaatcttcgg cccgctgctg ccgctgcggc cgtatcgcga cctcgacgcg gcgatcgcct
      901 tcatcaacag ccggccgcgg ccgctggcgc tgtactactt cgaccgcgcg cccgccaaca
      961 tcgaacgtgt gctcaacgaa acggttgccg gcggcgtgac gatcaacgac accatcctgc
     1021 acatcgcgca ggacgacctg cctttcggtg gcgttggccc gagcggtatg ggttgctacc
     1081 atggcttcga ggggttccag accttttcgg cgcgcaaggc cgtcttccgg caatcgcgcc
     1141 tgtcggcgat cggtctcttc aagccgccgt acggcgctgt gttcgagcgt ttgacgagga
     1201 ttctgctgcg atgattgcca cgcgccgaca gttcctcaag accggcgtgg ccggcagcct
     1261 gttgctcggt tgcgcgggct ggctcaacgc cgccggcgcg cgcccgctca gcgatgccga
     1321 gcgcgagatg ctcggcgcgc tgaccaacgc cctgctcgac ggtgcgctgc cgacagacct
     1381 tgccgcccgc cggcgcctga tcgccgtgac catcgacggc atcgccgtgg aaatcgccgg
     1441 tctgtccctg gccagccaga aggagatcgg cgagttgttc ggtctgctgg tgcttgctcc
     1501 cggcagacgg ttcctggccg gggtcggcag gccgtggcgc gaggcggccg ctggcgacgt
     1561 cgtcgagttt ctgcaagcct ggcgatcgag ccgtctgagc ctgctgcaga gcgcgtacgc
     1621 ggcactgcac gacctcacct ttggtgcctg gtacgcgcgc ccggacacct gggaggccat
     1681 cggctatccc ggcccaccac gggggtattt ctgatgcagg atccgatcaa ggccggcctg
     1741 gctgccggct ggaagcacat cgacgcgtcg ctgctcgcca gcgacctgag catcgagacc
     1801 gacgtggtca tcgtcggcac cggcgccggc ggcggggtca ccgccgacat tctcagcgac
     1861 gccggcttgc gggtcgtgct cgtcgaggaa gggccgttac gctcgtcgag cgacttcagg
     1921 atgcgcgagg ccgacgccta tccggaactg taccaggagt cggcgtcgcg caagaccgcc
     1981 gacaaggcga tcaacatcct gcaggggcgc tgcgtcggcg gttcgacgac ggtcaactgg
     2041 accagcagct tccgcacgcc accggaagtc ttcgactatt ggcaacgcca gttcggactg
     2101 cgacaactga gcgtcgagtc gatgagttcg tggtttgcgg tgatggagca caagctctgg
     2161 gtacgcgcct gggagacgcc gccaaacgcg aacaacaagg cgctcgccgc cggtgccgaa
     2221 aaactcggca tcccgatcgg ctccatcaag cgcaacgtca agggctgctg gaacctcggc
     2281 tactgcggca tgggctgccc gaccaacgcc aagcagtcga tgttgctgac caccattccc
     2341 gccgcgctgc agcgaggggc gaccttgtgc acccgcttgc gggccgagcg cctgcgtttc
     2401 accggcagcc gcgtcaccgc cctcgactgc gtcgctctgc aagcggacgg catccatccg
     2461 ggcaggcagc gggtgcacct gcgcgcgcgc cacttcgtca tcgccggcgg ggcgatcggc
     2521 agcccggcgc tgctgctgcg cagcggcgtc cccgatccgc agcggctgct cggcaagcgc
     2581 accttcctgc atccggtggt gatttcgtcg gcgctgatgc ccgagcgcgt cgatgcctac
     2641 gccggcgcgc cacagtcgat ctacaccgac cattttctgc atcaggcgcc gatcgacgga
     2701 ccgctcggct tcaagctcga aacaccgccg ctgcacccgg tgctctatgc cacgacgctg
     2761 cagggttttg gcgaggcgca cggcaagctg atgcgcgaat tcacccaggt caacgtgctg
     2821 ctggcactgc tgcgcgacgg cttccatccc gaaagccgtg gcggccaggt gcggctcggc
     2881 agcgacggcc tgccggtgct cgactacccg ctgggcgacg tgatctggga ggcggcgcgg
     2941 cgcgcgctgc tggcgatggc cgaaatccag ttcgccgccg gcgcgcgctg ggtcaccccg
     3001 gtgcacgaga cggtgcccgg ctatgcgagc tgggccgagg ccaggaaagg gatcgccgag
     3061 ctgccactca agccgctgct gtgccgggtg gtcagcgcgc acgtgatggg cggatgcggc
     3121 atggccgaca ccccgcagcg cggcgtcgtc gatcaccgtg gccggcactt ctggctcggc
     3181 aacctgtcgg ttcacgacgg ttcggtcttt ccgaccagcc tgggggccaa tccgcaactt
     3241 tcgatctacg gcctggtcgc ccgcaacgcc agcctgctgg ccgccgagct gtccggccgg
     3301 ccgctgccgg cgatcctctg aaggagtcct cccgcatgct gtcgcgcgcg cttcgccgat
     3361 cgctggccct ggaagtggcg ctctacgtcg cgctcgccat ttacggatgt gacgcctcgc
     3421 cagcggctgc cgccggcgtc gcgctggccg gcgtgctggc cctgcgggcg acgctgacgg
     3481 cggtgaccta cgcctacgcc tgggcctgcc attcgcctgc gccgcccctc tcgccggggc
     3541 aggccgtgcg catggtgctc agcgagtacg cggcctgcgt gttcagcttc gtgctgattt
     3601 ttccgttcga gcgctggtgg atggctcccg accgcctgca aaccggtgcc gaaccgaccg
     3661 ccgggcggcc cgatgcgcgc ccgccggtgc tgctggtgca tggctacagt tgttcgcgtg
     3721 ccgcctggtg gtggctgcgt tgccgcctcg aagccgccgg ctggcgcgtg gcgacgatca
     3781 gcctcgaacc gatctacggc agcatcgacg agtacgtcga gccgctggcg cggcgcatcg
     3841 ccgccgtcct ggccgaaacc ggcgccgagc gcctgctgct cgtcggccac agcatgggcg
     3901 ggctggtcgc gcgcgcctat ctgcagcgcc acggcgcggc gcgggtggcc aggctggtca
     3961 ccctcgggac gccgcatcag ggcagccgca tcgcgcacct cggtcttggc gagaacgccc
     4021 gccagatgca gcccgacagc gcctggctgc aggcccttgc cagcccggcg ccggtgctcg
     4081 atactcgggt catctacagt ccgcacgaca atttcgtgat gccgcaagag cgattgcaac
     4141 tgccgggagc caccagtcag gcgatcgccg gcgtcggtca cctggcgatg ctctattcgc
     4201 cgcgcgtggc gcgggcgctg ctggcggcgc tcgccggcac cagcaacacc ggcgccgcct
     4261 gagccgctga tgcgcgcgcg cgtcctgctg ctgccgctca gcctgctgtc ggccctgccg
     4321 tctgccgcgc cggcgcagga cgtgttcgtc acccgcggcg ccggcagccc ggtcttttcc
     4381 gacaagccgc aggccggcgc caagccactg agcctgccgc cgctcaacgt gatcgagccg
     4441 ctgccggtgt ccagggcgcc ggccgcggcc acgccagcgc gggaagaaag ccgccgggcg
     4501 gagccggcaa taccggccta ccagaagttc agcatcgtct cgcccgcgaa cgatggcagc
     4561 gtgctggcca acactgcgct tttcgaggtg cgggtcgtcg tcgagccgcc gctgcaactc
     4621 ggcgaggggc acgcggtcac ggtcagcatc aacggccggc cggtcggcca gcgcttcacc
     4681 gccggcgagt tcatgattcc gcccgagttc tggggcgaca ccctgccgcc agccaaccag
     4741 cgccaccagc tcgacgcggc gatcgtcgac cgcaatggtg cggtgctcag gcgggccgag
     4801 ccggtgacct tccacctgcg ctacgtcgcc ggcctgcagc accggcatgc gccgcggtca
     4861 gccccgatgc cgatcccggt cgacaagccc aggccgccgg cgcaaccaga acccttgatc
     4921 agaaaatttg tgcagccggc caggcccctg ttcgagcatt gagtcactcg tggcttcggc
     4981 acgtctatca ctcgtgtcgc aaccggcatt gcccgcaatg ccagacccg
//
LOCUS       DAOSIT010000111         5017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_14927_length_5017_cov_12.038694, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000111 DAOSIT010000000
VERSION     DAOSIT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14927_length_5017_cov_12.038694"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLL14_03970"
     CDS             complement(<1..193)
                     /locus_tag="PLL14_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03111.1"
                     /translation="MPAAALPRPDAQGCRGDPRHGSADASAARFDHHSFSEGQTMSQL
                     VHQLPLSAVAEAPAIQPLAE"
     gene            complement(457..1875)
                     /locus_tag="PLL14_03975"
     CDS             complement(457..1875)
                     /locus_tag="PLL14_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOG03112.1"
                     /translation="MHFETPLEFLESRTYDEINVGDSSSLVRTLRPEDVKLFALMSGD
                     MNPALASTEFRHSGLFQDFIAHGMWTASLMSTTLGTQFPGPGTVLIESMVHFSRPVTI
                     GDTITVTVTVKQKFSHNQHIILDCACVNQDQLLVVSGSAEVLAPREKIRTKRTVMPEI
                     TISDKYARLQVLVKRAEGLAPIDMAVVHPCDRESLRGALLAAEANLIDPILIGPEQKI
                     RSVAEENGFDVGRHRIVNVRHSHEAAAMAVTLIRSNDAEALMKGSLHTDELMAEVIAS
                     GTGLRTERRISHVFLMDVPTYPRPVMITDAAVNILPTLLEKVHIIQNAIDLAHIIGNP
                     EPKVAILSAVETVNPKIQSTIDAAALCKMADRGQIKGGMLDGPLAFDNAVSLVAAKTK
                     GISSAVAGRADILVVPDLESGNMLAKQLEYLGNALSAGIVLGARVPIVLTSRADTAET
                     RIASCVIAALIAHAARERQPAS"
     gene            2221..4062
                     /locus_tag="PLL14_03980"
     CDS             2221..4062
                     /locus_tag="PLL14_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG03113.1"
                     /translation="MTDECVGAERLGELLQPIIDGHRGDPGMLVQILREAQEVLGYLP
                     AAALTAIARAVGLPRARVEGVAGFYSFLHLRPVGRYRVLFSDNITDRMLGSAELMDRL
                     CNRLWIERGKVSEDGLVSVDTTSCTGLCDQGPALLVNGRAMTRMSAERIDRISELIRA
                     ELPLDDWPREYFRIEDNIRRRDALLNTCWPAGEALRAALARGPEAMLAEMRTSNLRGR
                     GGAGFTTYIKWESARRAPCRGEPPERYIVCNADEGEPGTFKDRVLLSSYTDLLFDGMS
                     VAGFTIGATRGLLYLRGEYAYLLPALRENLARRRRDGLLGTALCGQDGVCFDIEIHVG
                     AGAYVCGEESALLESLEGKRGVPRIRPPFPVTVGYRGQPTVVNNVETLCKAALIAVNG
                     GAWFAGLGTKQSTGTKLLSISGDVEMPGIYDYPFGVSVAQVLNECGAGNAQAVQISGP
                     SGTCLGMHEFSRHVAFEDVPTAGAFMVFGAHRDMFEVARNFAHFFAHESCGFCTPCRV
                     GTSLVANCMDKIAEGHGSQYDINEIFKIHRLLHVASHCGLGQSACNPLFDTLNKFRPA
                     YERRLRSLDFEPAFDLDQALAAARQMTGRDDPAAHFGLAARVPGVTT"
     gene            4059..4790
                     /locus_tag="PLL14_03985"
     CDS             4059..4790
                     /locus_tag="PLL14_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG03114.1"
                     /translation="MTQQSILLDGLPVPFEEGQTIMDAAMAAEIFIPHLCHHPEFKPQ
                     GSCKLCTVKASGRYVSACTMPARDGMEVENNSPDLNDKRRTMLQMLFVEGNHFCPSCE
                     QSGNCMLQALAYELEMLSPHFNQFYPNRPLDASHPQVLLDFNRCILCELCVRASRDVD
                     GKHVFSLSGRGITKHLIVNAESGRLADTDFSLTDKAAHVCPVGVILEKRKGFAVPIGK
                     RRYDVDPIGMQVERLAHRTTTEEEI"
     gene            4792..>5017
                     /locus_tag="PLL14_03990"
     CDS             4792..>5017
                     /locus_tag="PLL14_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOG03115.1"
                     /translation="MEAVVQNRKLRLATTSLAGCFGCHMAFLDIDERLLDLVERVDFD
                     RSPLTDIKQCGACDIGLIEGGICNAENIHVL"
BASE COUNT          815 a         1564 c         1706 g          932 t
ORIGIN      
        1 gttcggcgag cggctggatc gccggcgcct cggcaaccgc cgacaggggt aactggtgta
       61 caagctggct catggtctgt ccctcgctga atgaatgatg gtcgaagcgg gcggcgctgg
      121 catccgcgct gccgtgccgt ggatcgccgc ggcagccctg cgcatcgggc cgcggcaggg
      181 ccgcggctgg catggcgtaa cagacgcaaa gctactacag gtagtggtac aaggcaacaa
      241 ggtaactaga tgtagttatc cacagcgccg gccgcggcga ccggaaaagc cgtgaaatca
      301 cggtcttgcg gcgcatttga gaagcgaaat cttttttccg gttcgcctgc gggtgcggcg
      361 aggggcgcgc gtccggcagg cgtgcgccgt cttcggggga cccgcctgcg ccggggtacg
      421 gctcgcgatc gctgccgccg cgagccgtct gccggatcag ctcgccggct gccgttcgcg
      481 cgcggcgtgc gcgatcagcg cggcgatgac gcaggaggcg atgcgtgttt cggcggtgtc
      541 ggcgcggctg gtcaggacga tcggcacgcg cgcgccgagg acgatgccgg cactgagcgc
      601 gttgccgaga tactcgagct gcttggcgag catgttgccg gattcgaggt cgggcacgac
      661 gaggatgtcg gcgcgtccgg cgacggccga gctgatgccc ttggtcttgg cggcgaccag
      721 cgagacggcg ttgtcgaaag ccagcggccc gtcgagcatg ccgcccttga tctggccgcg
      781 gtcggccatc ttgcacaagg cggcggcgtc gatggtggac tggatcttgg gattgacggt
      841 ttcgaccgcc gagaggatcg cgaccttggg ttcggggttg ccgatgatgt gcgccagatc
      901 gatcgcgttc tggatgatgt gcaccttctc cagcagcgtc ggcaggatgt tgaccgccgc
      961 gtcggtaatc atcaccgggc gcgggtaggt cggcacgtcc atcaggaaga catggctgat
     1021 gcgccgctcg gtgcgcaggc cggtgccgct ggcaatcacc tcggccatca gttcgtcggt
     1081 gtgcaggctg cccttcatca gcgcttcggc gtcgttgctg cggatcaggg tcaccgccat
     1141 ggccgccgcc tcgtggctgt gccggacgtt cacgatgcgg tgtcgcccga cgtcgaagcc
     1201 gttttcttcg gcgaccgagc gaatcttctg ttccgggccg atcaggatcg ggtcgatcag
     1261 gttggcctcg gcggcgagca gggcgccgcg cagcgattcg cggtcgcagg gatgcaccac
     1321 ggccatgtcg atcggcgcca ggccttcggc acgcttgacc aggacctgca ggcgcgcgta
     1381 cttgtcggag atggtgattt ccggcatcac cgtgcgcttg gttctgatct tctcgcgtgg
     1441 cgccagcacc tcggcgctgc ccgaaaccac cagcaactgg tcctggttga cgcaggcgca
     1501 gtcgagaatg atgtgctggt tgtggctgaa cttctgcttg accgtcaccg tcacggtgat
     1561 cgtgtcgccg atcgtgactg gccgggagaa atgcaccatc gactcgatca ggacggtccc
     1621 cggtcccggg aactgcgtgc cgagggtggt cgacatcagt gaggcggtcc acatgccgtg
     1681 cgcgatgaag tcctggaaca ggccgctgtg cctgaactcg gtactggcca gcgcgggatt
     1741 catgtcgccg gacatcagcg cgaaaagctt gacgtcctcc gggcgcagcg tgcgcaccag
     1801 cgacgaactg tcgccgacgt tgatttcgtc gtaggttcga ctttcgagaa attcgagggg
     1861 tgtctcaaag tgcatgatgg tgctcctgtt gacgctcacc ggagtgatcg gaacgcggtc
     1921 ggaatcggga ctacccgcca ccgccatcgg gtccgcgtgc cgcctcgcct gtcggcgatc
     1981 cgttcagccg gccgctgcgt ggcgcatgac gggcctgacg gtgacacacg gtcagacatt
     2041 attatgctgc aatgcagcac gaaatgaaat ggtgatctgc gggagtttgc tccgccgctg
     2101 cgcgtctgtc cgggaaccgc tcaatcggcg cttttgttga cgtacatcag tacccgccat
     2161 cgccgaagca gtaggctgca gcaaggtatc gggaacggct gcggttttcg ggagactggc
     2221 gtgacggacg aatgcgttgg ggcagaacgg ctgggcgagt tgttgcagcc gatcatcgac
     2281 ggtcaccgcg gtgacccggg gatgctggtg cagattctgc gcgaggcgca ggaagtgctg
     2341 ggctacttgc cggcagcggc gctgaccgcg atcgccaggg cggtcgggtt gccgcgcgcg
     2401 cgcgtcgagg gggtcgccgg tttttactcg tttctccacc tgcggccggt cggtcgctat
     2461 cgcgtgctct tctcggacaa catcaccgac cgcatgctgg gcagcgccga gttgatggat
     2521 cgcctgtgca accggctgtg gatcgagcga ggcaaggttt ccgaggacgg gctggtcagc
     2581 gtcgacacga cttcctgcac gggcctgtgc gaccaggggc cggcgttgct ggtcaacggc
     2641 cgggcgatga cccgcatgtc ggccgagcgc atcgaccgca tcagcgaact gatccgggcg
     2701 gaactgccgc tggacgactg gccgcgcgag tatttccgga tcgaggacaa catccgccgc
     2761 cgcgatgccc tgctcaatac ctgctggccg gccggagaag ccctcagggc cgcgctggcg
     2821 cgcggacccg aggcgatgct cgcggaaatg cggacctcga acctgcgcgg gcgcggcggc
     2881 gccggtttca ccacctacat caagtgggag tcggcacggc gcgcgccctg ccgcggcgag
     2941 ccgcccgagc gctacatcgt gtgcaacgct gacgaaggcg aaccggggac cttcaaggat
     3001 cgcgtgctgc tttcgagcta caccgatctg ctgttcgacg gcatgagcgt cgccggtttc
     3061 accatcggtg cgaccagggg tctgctctac ctgcgtggcg aatacgccta cctgttgccg
     3121 gcgctgcgcg aaaacctcgc ccgtcgccgc cgcgacggtc tgctgggaac ggcgctgtgc
     3181 ggccaggacg gcgtgtgctt cgacatcgag atccatgtcg gtgccggtgc ctatgtctgt
     3241 ggcgaggaat cggcgctgct cgaatcgctt gagggcaagc gcggcgtgcc gcggattcgt
     3301 ccgcctttcc cggtcaccgt gggctatcgc ggtcagccga cggtggtgaa caatgtcgaa
     3361 acgctgtgca aggcggcgct gatcgcggtc aatggcggcg cctggttcgc cggtctcggc
     3421 accaagcagt cgaccggcac caagctgctg tcgatctcgg gggacgtcga gatgccgggc
     3481 atctacgatt atcccttcgg cgtctcggtg gcgcaggtgc tcaacgaatg cggcgccggc
     3541 aatgcgcagg cggtgcagat cagcggtcct tccggcacct gcctcgggat gcacgagttc
     3601 tctcgtcacg tggctttcga ggacgtgccg accgctggcg cgttcatggt cttcggcgcg
     3661 caccgcgaca tgttcgaagt ggcgcgcaat tttgcccact tcttcgcgca cgagagctgc
     3721 ggtttctgca cgccctgccg ggtcggcact tccctggtcg ccaactgcat ggacaagatc
     3781 gccgagggtc atggttcgca gtacgacatc aacgagattt tcaagatcca tcgcctgttg
     3841 cacgtcgcca gccactgcgg gctcggccag tcggcgtgca accccttgtt cgatacgttg
     3901 aacaagtttc gtccggccta tgagcgacgc ctgcgctcgc tcgatttcga gcccgccttc
     3961 gatcttgacc aggccctggc ggcagcacgc cagatgaccg ggcgcgacga ccccgcggcc
     4021 cacttcggtc tggccgccag ggttccggga gtgaccacat gacacagcag agcattctcc
     4081 tcgatgggct gccggtgccc ttcgaggaag ggcagacgat catggatgcg gcgatggctg
     4141 ccgagatttt catcccgcac ctctgtcatc atccggagtt caagccgcag ggcagttgca
     4201 agctgtgtac cgtgaaggcc agcggccgct acgtttccgc gtgcacgatg ccggccaggg
     4261 acggcatgga ggtggagaac aactcgccgg atctcaacga caagcgccgg acgatgctgc
     4321 agatgctgtt cgtcgagggc aatcacttct gcccgtcctg tgaacagagc ggcaattgca
     4381 tgctgcaggc gctggcctac gaactcgaaa tgctgtcgcc gcatttcaat cagttctacc
     4441 ccaatcgacc gctcgatgcc tcgcacccgc aggtgttgct cgacttcaat cgctgcattc
     4501 tctgcgaact gtgcgtgcgc gccagccgcg acgtcgacgg caaacacgtc ttctcgctct
     4561 cggggcgcgg gatcaccaag cacctgatcg tcaatgccga gtccggacgc ctggccgata
     4621 ccgatttttc gctgaccgac aaggcggcgc acgtctgccc ggtcggggtg atcctcgaaa
     4681 aacgcaaggg cttcgcggtg ccgatcggca agcggcgcta cgacgtcgat ccgatcggca
     4741 tgcaggtcga gcgcctcgcg caccgcacga ccactgaaga ggagatctga gatggaggct
     4801 gtggttcaga accgcaagct gcgtctggcg acgacttcac tggccggctg cttcggttgc
     4861 cacatggcgt ttctcgacat cgacgaacgg ctgctcgatc tggtcgagcg ggtcgacttc
     4921 gaccgctcgc cgctgaccga catcaagcaa tgcggcgcct gcgacatcgg cctgatcgaa
     4981 ggcggcatct gcaacgccga gaacatccac gtgctgc
//
LOCUS       DAOSIT010000112         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15061_length_4982_cov_8.546783, whole genome shotgun sequence.
ACCESSION   DAOSIT010000112 DAOSIT010000000
VERSION     DAOSIT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_4982_cov_8.546783"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1324
                     /locus_tag="PLL14_03995"
     CDS             137..1324
                     /locus_tag="PLL14_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG03116.1"
                     /translation="MSGLRVIDVGTFLAGPYAASILGEFGAEILKVEHPIAGDPMRRF
                     GTASKRHDATLAWLSEGRNRKSVTLDLRQQEGVTLFLKLVAKSDVLIENFRPGTMEEW
                     GLGWDELSKANPGLVMLRVSGYGQTGPYRRRSGFAHIAHGFAGLSYLAGFPGETPVVP
                     GTAPMGDYLSSLYGAIGILLALRHREATGEGQIIDIGIYEAVFRQMDEIAAAYGLFGK
                     VREREGAGSFVAVPHGHFRCIDDKWVAIACTTDKMFERFAAVMGKPELAAADRYGDQR
                     KRLAARDEVNRIVVEWVGSMTRKEVMQRCIDGEVPAGKLNSIADIFEDEHFQARGNLA
                     RLREEGLGEVVVPGVVPTLSETPGRISNLGPPLGNATYEVLRELLGISAQEIKRLRQH
                     KII"
     gene            1347..2573
                     /locus_tag="PLL14_04000"
     CDS             1347..2573
                     /locus_tag="PLL14_04000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOG03117.1"
                     /translation="MHTQPTASRLPLEGIRVIDAATVIAAPYSATILSEFGAEVLKVE
                     NPIGGDPMRHFGTPTQRKDTLAWLSEARNKKSVTLDLHLPEGVELFKKLVEKSDVLCE
                     NFRPGTLEKWGLGWKVLHAINPRLIMLRVTGYGQTGPYRDRPGFARVAHAVGGIAFLA
                     GMPRGTPVTPGSTTLGDYLTGLYGCIGILMALRHRDRTGQGQYIDAALYESVFRCTDE
                     LAPAYGMFGRVRERHGPTHNDFACPHGHFPTKDDGKWVAISCVTDKLFERLAIAMGRP
                     ELAAPHVYGDQKTRLESRHDVNEIVRDWCGSLHREELLNRCFAAGAPAGPLNSIADIF
                     GDRQFHARRNLVAIDEADIGETVIVPSVLPRLSATPGRIKHLGPRLGQHTDEVLSGLL
                     GLEAHEIEGLRKKRVI"
     gene            2597..3817
                     /locus_tag="PLL14_04005"
     CDS             2597..3817
                     /locus_tag="PLL14_04005"
                     /EC_number="5.4.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylfumaryl-CoA isomerase"
                     /protein_id="HOG03118.1"
                     /translation="MDGILKGLRVVEGSAFVAAPLGGMTLAQLGAEVIRFDPIGGGLD
                     YRRWPLTRDGQHSLFWAGLNKGKRSIAVDFRKPRGQELLTQLICAPGENAGLFSTNFP
                     AKGWLSYEKLQAHRQDLIMINLTGRRDGGSEVDYTVNPQIGLPFMTGPTHSPDVVNHV
                     FPAWDFISGQMIAVGMLAAERHRRLTGEGQLVRLALKDVALAMLGNFGMLAEVMVNDT
                     DRPRQGNYLYGAFGRDFETLDGKRLMVVGLTDMQWQCLTRATGLQEAIAALGTRLGLD
                     LEDEGNRFRARQQIAALLEPWFHARTLAEAAEILNAHRVTWGPYRSVREALACDPDCS
                     VDNPLFTLTEQPGIGSCLMPATPLEFGRSPRLPAMPAPALGEHTDQILLDILGLSAAE
                     VGRLHDQGVVAGPA"
     gene            3885..4928
                     /locus_tag="PLL14_04010"
     CDS             3885..4928
                     /locus_tag="PLL14_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOG03119.1"
                     /translation="MKLPVHFYKPLAIGAPQPLRELPVRPERMIHFFPPHIDKIRAKA
                     PETARQCDVMCGNLEDAIPIEAKDAARAGFIDLLASHDFGDTGMWVRINALNSPWVLD
                     DLNEIIRHVGNKLDVIMIPKVEGPWDIHFVDQYVALLEAKYAIRKPILLHALLETAQG
                     VTNVEAICGASPRMHGLSLGPADLAASRGMKTTRVGGGHPGYGVLADPEDGQDQRAFF
                     QQDLWHYTVARMVDAAVTHGLRSFYGPFGDLKDEAACEAQFRNAFLMGCSGAWSLAPN
                     QIAIAKRVFSPDIKEVLFAKRILEAMPDGSGVATIDGKMQDDATWKQAKVIVDLARLV
                     ARRDPELAAAYGL"
BASE COUNT          955 a         1623 c         1579 g          825 t
ORIGIN      
        1 ggcgacatcc gcctcgttgc ggtctggcgt cgaggagaaa gaggacgaga cgccggcagt
       61 tgatcgtacc tcgatcaacg atgaacggca gaacgacccg gatcaggaac gctcggccga
      121 aaacgacaag ctgccgatga gcggtctgcg ggtgatcgac gtcggcacct tcctggctgg
      181 tccctatgca gcgtcgatcc tcggcgagtt cggagccgag attctcaagg tcgagcaccc
      241 gatcgccggc gatcccatgc gccgcttcgg cactgcgtcg aagcgccacg acgcgacgct
      301 ggcctggctc agcgaaggcc gcaacaggaa atcggtcacc ctcgacctgc gccagcagga
      361 gggtgtcacc ctgttcctga aactggtcgc caagtctgac gtcctgatcg agaacttccg
      421 ccccggcacg atggaggaat ggggactcgg ctgggacgag ttgagcaagg ccaaccccgg
      481 tctggtcatg ctccgggtct cgggctacgg acagaccggg ccgtatcgcc gacgctcggg
      541 gttcgcgcac atcgcccacg gttttgccgg tctgtcgtac ctcgcaggtt tccccggcga
      601 gactccggta gttccgggca cggcgcccat gggtgactat ctgtccagcc tctacggcgc
      661 cattggcatt ctgctggcgc tgcgccaccg ggaagcgacc ggcgagggac aaatcatcga
      721 catcgggatc tacgaggcgg ttttccgcca gatggacgag atcgccgcgg cttacggcct
      781 gttcggcaag gtccgcgagc gcgaaggcgc aggcagcttc gtcgccgttc cgcatgggca
      841 cttccgctgc atcgacgaca agtgggtagc catcgcgtgt actacggaca agatgttcga
      901 acgcttcgcc gcagtgatgg gcaaacccga actcgccgcc gccgatcgct acggcgatca
      961 gcgcaagcgt ctggcggcgc gcgacgaggt caatcgcatc gtcgttgaat gggtcggctc
     1021 gatgacgcgc aaggaagtca tgcagcgctg catcgacggc gaagtaccgg ccggcaagct
     1081 caacagcatc gccgacattt tcgaggatga acacttccag gcccgtggca acctggcccg
     1141 gctcagggaa gaaggcctcg gcgaagtggt cgttccgggg gtcgtgccca ccttgtccga
     1201 aacacccggc aggatttcca acctcggccc gccgctcggc aacgcgacct atgaggtgct
     1261 gcgcgaactg ctggggattt ccgcacagga aatcaagcgg ctgcggcaac acaagatcat
     1321 ctgaaccgac catcgcgaga acccccatgc acacccaacc gaccgcgagc aggctgccgc
     1381 tcgaaggcat tcgcgtcatc gacgccgcga cggtcatcgc cgccccctac agcgcgacca
     1441 ttctgagcga gttcggcgcc gaagtcctga aggtcgaaaa cccgatcggc ggcgacccca
     1501 tgcggcactt cggaacgccg acccagcgca aggacacgct ggcctggctc agcgaagcac
     1561 gtaacaagaa atcggtgacg ctcgacctgc acctgcccga aggcgtcgaa ctgttcaaga
     1621 aactggtcga gaaatcggac gtgctgtgcg aaaatttccg gcccggcacc ctagaaaaat
     1681 ggggcctcgg gtggaaggtg ctgcatgcga tcaacccacg gctgatcatg ctgcgcgtca
     1741 ccggctatgg gcaaaccggc ccgtaccggg atcggcccgg ttttgcccgc gtcgcgcacg
     1801 cggtcggcgg catcgccttt ctcgccggca tgccacgcgg aacaccggtg acgccggggt
     1861 caaccaccct cggtgattac cttaccggtc tgtacggctg catcggcatc ctgatggcct
     1921 tgcgccaccg cgaccgcacc ggccaggggc agtatatcga cgccgcgctg tacgagtcgg
     1981 tctttcgctg tacggatgaa ctggcgccgg cgtacgggat gttcggccgg gtgcgcgagc
     2041 ggcacgggcc gacgcacaac gacttcgcct gcccgcacgg gcatttcccg accaaggacg
     2101 acggcaagtg ggtggccatt tcctgcgtca ccgacaagct cttcgagcgc ctggcaatcg
     2161 ccatgggacg ccccgaactg gcggcgccgc acgtctatgg cgaccagaag acacgcttgg
     2221 agagccgtca tgacgtcaac gaaatcgtcc gcgactggtg cgggtcgctg caccgcgagg
     2281 aactcctcaa tcgttgcttc gccgccggcg cgccggcggg tccgctgaac agcatcgccg
     2341 acattttcgg cgaccggcag tttcacgccc ggcgcaacct ggtggccatc gacgaggcgg
     2401 acattggcga gacggtgatc gtaccgtcgg tactgccgcg tctgtcggcg acaccggggc
     2461 gcatcaagca cctcggtccg cgcctgggac agcataccga tgaagtactc tccggtctgc
     2521 tgggtctgga agcgcacgag atagaagggc tgcgcaagaa gcgcgtcatc tgaacgcggc
     2581 aggaggtatg gaaagcatgg acggcattct caagggattg cgagtcgtcg agggatcggc
     2641 tttcgtcgca gcgccactgg gcgggatgac cctggcccaa ctcggagcgg aggtcatccg
     2701 tttcgacccg atcggcggcg gcctcgacta tcgccgctgg ccactgaccc gcgacggcca
     2761 gcacagcctg ttctgggccg gcctgaacaa gggcaagcgc tcgatcgccg tcgactttcg
     2821 caagccgcgc gggcaggagt tgctgacgca gttgatctgc gcaccgggcg aaaacgccgg
     2881 cctgttcagc accaacttcc cggcgaaggg ctggctgagc tatgaaaagc tgcaagctca
     2941 tcgccaggat ctgatcatga tcaacctgac aggtcgccgc gacggcggct cggaggtgga
     3001 ctacacggtc aacccgcaaa tcggcctgcc cttcatgacc gggccgaccc actctccgga
     3061 cgtggtgaac cacgtctttc cagcctggga cttcatcagc ggccagatga ttgccgttgg
     3121 catgctggcc gccgaacggc atcggcgact caccggcgaa ggacagctgg tacggctggc
     3181 gctcaaggac gtggcgctgg cgatgctcgg caatttcggc atgctcgccg aggtgatggt
     3241 caatgacacc gaccggccac gccagggcaa ctacctctac ggcgcgtttg gccgcgactt
     3301 cgaaaccctc gacggcaagc gcctgatggt cgtcggtctg accgacatgc agtggcagtg
     3361 tctcaccagg gccaccggtc tgcaggaagc gatcgcggcg ttgggcacac ggcttggcct
     3421 cgacctggaa gacgaaggta atcgcttccg tgcccgccag cagattgccg cgctgctgga
     3481 gccgtggttc cacgcgcgca cgctcgccga agcggcagag atcctcaatg cccaccgggt
     3541 cacctggggc ccctaccgca gcgtgcgtga agccctggcc tgcgacccgg actgctcggt
     3601 cgacaatccg ctgttcaccc tgaccgaaca gccgggcatc gggtcctgcc tgatgccggc
     3661 cacgccgctc gaattcggtc gttcaccgcg cctgccggca atgccggcgc ccgccctcgg
     3721 cgagcatacc gaccagattc tgctcgacat cctcggcttg agcgcagccg aagtcggccg
     3781 cctgcacgat cagggcgtcg tcgccgggcc cgcctgaata tcaaagccgc cgcagagagc
     3841 aagcactctg cggtgtatcc acccacctct gcagggaaca aaccatgaag ctacccgttc
     3901 atttctacaa gccactcgcc atcggtgcgc ctcaacccct gcgtgaactg ccggtgcggc
     3961 cggaacgaat gatccacttc tttccgccac acatcgacaa gatccgcgcc aaggcgccag
     4021 aaaccgccag gcaatgcgat gtgatgtgcg gcaacctcga ggatgccatc ccgatcgagg
     4081 ccaaggatgc ggcgcgcgcc ggtttcatcg acctgctggc cagccatgac ttcggcgaca
     4141 ccggcatgtg ggtgcgaatc aacgccctga acagtccctg ggtactcgac gacctcaacg
     4201 agatcatcag gcacgtcggc aacaagctcg atgtgatcat gattcccaag gtcgaagggc
     4261 catgggacat tcatttcgtc gatcagtatg tcgccctgct cgaagcgaaa tacgcgatca
     4321 ggaagccgat tctgctgcat gcgctgctcg aaacggcgca gggagtgacc aacgtcgagg
     4381 cgatctgcgg cgccagcccg cgcatgcatg gtctgagcct gggcccggcc gatctggccg
     4441 cgtcgcgggg catgaagacg acccgtgtcg gtggtggcca tccgggttat ggcgttctcg
     4501 cggaccccga ggacggccag gatcaacgcg cgttctttca gcaggacctc tggcattaca
     4561 ccgtcgcgcg catggtcgat gccgcggtga cgcatggcct gcgctcgttt tatggcccgt
     4621 ttggcgacct caaggacgaa gcggcgtgcg aggcgcagtt ccgcaacgcc ttcctgatgg
     4681 gctgctccgg cgcctggtcg ctggccccga accagatcgc cattgccaag cgtgttttca
     4741 gccccgacat caaggaagtg ctgtttgcca aacgcatcct tgaagccatg cccgatggca
     4801 gcggcgtggc gacgatagac ggcaagatgc aggacgatgc gacctggaaa caggccaagg
     4861 tcattgtcga cctggcgcgg ctggtggcca ggcgagatcc tgaactggca gcagcctatg
     4921 gcctgtagac tctttactca aatgcacggg gagaaagtca agtgagcgaa aagaccagac
     4981 tg
//
LOCUS       DAOSIT010000113         4982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15063_length_4982_cov_5.358027, whole genome shotgun sequence.
ACCESSION   DAOSIT010000113 DAOSIT010000000
VERSION     DAOSIT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_4982_cov_5.358027"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(569..826)
                     /locus_tag="PLL14_04015"
     CDS             complement(569..826)
                     /locus_tag="PLL14_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03120.1"
                     /translation="MTTTSAERQQAYRAKRPFAGDNGERRLQSWVLTATALALGRLAR
                     RAGTTQRAVLEQLILDADRAVTQGMSDDELDHYLALQRNNE"
     gene            complement(840..1601)
                     /gene="istB"
                     /locus_tag="PLL14_04020"
     CDS             complement(840..1601)
                     /gene="istB"
                     /locus_tag="PLL14_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046622600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOG03121.1"
                     /translation="MLMQQTLLTLKSLRLPGMAAAFEEQQRLAQVAALAFDERFAMLV
                     DREHAYRENRRIARLLREARLKSSQACIEDIRYGTGRNLDRALMAQLASCQWIRAHQN
                     LLLTGATGCGKTWLACALGNAACRQGLSVLYSRLPRLLEELRIARGDGSFSRRLATLA
                     KIDLLILDDWAVAPLSQAERNDLLEILDDRVSTRSTVITSQLAPKHWHAFLNDPTLAD
                     AILDRILHAAHRLALSGESQRKLDGETSLLSSVTL"
     gene            complement(1619..3163)
                     /gene="istA"
                     /locus_tag="PLL14_04025"
     CDS             complement(1619..3163)
                     /gene="istA"
                     /locus_tag="PLL14_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG03122.1"
                     /translation="MPQERLSMRKIRDVLRYRHTTDLSLEAIARALKISKGVVAKYLR
                     LAEGASVGWPLPEDCDDLALERLLYRQPEARSSAFIGPDYARVHQELKKKGVTLALLW
                     EEYRQASGERGYQYTAFCTRYRAWAGKLKRSMRQIHRAGEKLFADYAGPTVPIIDGQT
                     GEIRSSSVFVAVLGASNYTFACATPQQTQADWLGGLARALTYMGGVTALIVPDNPRAM
                     VRDADPYEPVLNRLTEEFAAHYGTVILPARPRRPQDKAKVESGVQVVERWILARLRHR
                     QFFSVAEADAAIAALLPELNSRPFKKLPGCRASAFALLDAPYLRPLPATPFTLAEWKR
                     ARVNIDYHIEFDGSYYSVPHALIHREVELRVTQNTVEVLAGTRRVASHPRSPRRGHFE
                     TTPEHMPAAHRAHAEWSPGRLLNWAATIGPATAELVKRLLLEKPHPEQGYRACLGLMR
                     LARTYPHERLEAACTRALAVGAHRYRSVASILAKGLDQQPLTSPAQGELALPEHANVR
                     GPTYYH"
     gene            complement(3321..4097)
                     /locus_tag="PLL14_04030"
                     /pseudo
     CDS             complement(3321..4097)
                     /locus_tag="PLL14_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(4195..4536)
                     /locus_tag="PLL14_04035"
     CDS             complement(4195..4536)
                     /locus_tag="PLL14_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03123.1"
                     /translation="MSVRFLGRRVYLALLVVLGSARHAGQNPGAARLSATLAIPLRTL
                     ERWRQWWKDQFPLSTLWQASGARFMPPVTERFPTSLLERFVGDAAEAMGHLLVFLTPI
                     TVRPSALRGGR"
BASE COUNT          946 a         1498 c         1676 g          862 t
ORIGIN      
        1 ctgaatccga aattacgggc ttgtagtgcc acttgagttc ttcttggctc gcaactaatt
       61 gattaagtta tggtgcgtat ttcgcggaac gtgaccagtc gtttcgcgtg atcgtgaccg
      121 gtggagagat gctgcatgga tagggttgaa tgttacctca accggtcacg cctttgatga
      181 aatggcggtc acgctttcgt gaaatggatg agggtctggt cccgttacag ggtaacgagg
      241 ggtgcgttga gggggcggtc aagggcgggc gaagcccggc gaagcgaacc cttgagggcc
      301 ccctcaacgc ggatacgctc cagggcttgg ggctggttgc ggattttcgc agccggcccc
      361 cgagcccgtc cggcggcgag gacttccccg ccctgggggg cggggtcagg gggtggggga
      421 agcgccgggg tttgccaatg gcacgcgttt ttgcaaaggt cagcgtgggg tcgcagaccc
      481 gatcgcgcag gggtggaggg aggctgcagg ctgagcaaca taggccgagg acctttcatc
      541 aagcgttttt ggggggactg gcagatggct actcattgtt acgctgtaac gcgaggtagt
      601 gatccaactc gtcatcgctc attccctgcg tgacggcccg atcggcgtcg agaatgagtt
      661 gttcgaggac cgcacgttgc gtagtgccgg cgcggcgagc cagccggccc agggccagag
      721 cggtcgcggt caagacccag gattgcaggc gtcgttcgcc attgtcgcca gcgaaggggc
      781 gtttggcgcg ataggcttgt tgtctttctg cggaagttgt cgtcatgatt tgggcgtggt
      841 tacagtgtaa cggaggacag aagagaggtt tcgccatcga gcttgcgctg cgattcacca
      901 ctgagcgcga gccggtgggc ggcatgcagg atgcggtcga gaatcgcgtc agcgagagtg
      961 ggatcattga gaaaggcatg ccaatgcttg ggtgccaact ggctggtgat caccgtcgaa
     1021 cgggtactta cccgatcatc aaggatctcc agcaaatcat tacgctcggc ctgggacagc
     1081 ggggccacgg cccaatcgtc gaggatcagt agatcgatct ttgccagagt cgcgagacgt
     1141 ctgctgaagc ttccgtcgcc acgagcgata cgcaactcct cgagaaggcg gggcagacgt
     1201 gagtagagaa cggacagtcc ctgccggcag gcggcattgc ccagggcgca ggcgagccag
     1261 gtcttcccgc aaccggtggc gccggtcagg agcagattct ggtgtgcccg gatccactgg
     1321 caactggcca attgcgccat cagcgcacga tcgaggttgc gaccggtgcc gtagcggata
     1381 tcctcgatac acgcctgcga ggacttgagc ctggcctcgc gcaacagacg ggcgatgcgg
     1441 cgattctcgc gatacgcgtg ctcgcgatcg accagcattg caaagcgctc gtcgaacgcc
     1501 aaggccgcga cctgggcgag cctctgctgt tcctcgaagg cggcggccat gccgggcagg
     1561 cgcaaggatt tcagggtcag gagggtttgt tgcatcagca tgggggttcc tttcgggttc
     1621 agtggtaata ggtcggaccg cgcacattgg cgtgctccgg caaggccagt tcgccctgtg
     1681 cgggcgacgt gagcggctgt tgatcgagtc ccttcgccag aatcgaagcg accgagcgat
     1741 agcgatgggc gccgacggcg agcgcccggg tgcaggcggc ctccaggcgc tcatgcggat
     1801 aggttcgggc cagacgcatc aagcccagac aggcacggta cccttgctct ggatgcggct
     1861 tctccagcag cagacgcttg accagttccg ccgtagccgg accgatggtc gcagcccagt
     1921 tcagaaggcg accgggagac cattcggcat gcgcacggtg ggctgccggc atgtgttcag
     1981 gcgtggtctc gaaatgcccc cggcgagggc tgcgcggatg actggccacc cggcgcgtgc
     2041 cggccagcac ttcgaccgtg ttctgcgtca cgcgcagttc cacttcgcga tggatcaggg
     2101 cgtgcggcac gctgtaatag ctgccgtcga attcgatgtg atagtcgata ttgactcgcg
     2161 cgcgcttcca ttcagcgaga gtaaagggcg tcgccggcag gggccgcagg tagggcgcgt
     2221 cgagcagagc gaaggcagac gcccggcatc cgggcagctt cttgaatggg cgcgaattca
     2281 gttccggcag caacgccgcg atcgccgcgt cggcctcagc taccgagaag aattgccggt
     2341 gacgcagtcg cgcgagaatc cagcgctcga cgacctgcac gccactttcg accttggcct
     2401 tgtcctgtgg acgccggggg cgcgccggca gaatgacggt gccgtagtgg gcggcaaact
     2461 cctcggtgag gcggttcaac accggttcat agggatcggc atcgcgaacc atggcgcgcg
     2521 gattatccgg aacgatgagc gcggtgacgc cacccatata ggtcagcgcc cgcgccagtc
     2581 cgccgagcca gtcagcctgc gtctgctggg gcgtggcgca ggcgaacgtg tagttggacg
     2641 cgccgaggac ggcgacgaag acgctggacg atcgaatttc gcccgtttgg ccgtcaatga
     2701 tcggcacggt agggcccgcg taatcggcga agagtttctc accggcacgg tgaatttgcc
     2761 gcatcgagcg cttgagcttc ccggcccaag cgcgatagcg ggtgcagaac gccgtgtatt
     2821 ggtaaccccg ctccccgctc gcttgccggt actcttccca gagcagcgcc agcgtcacgc
     2881 ccttcttctt gagttcctga tgaacgcgcg cgtagtcggg cccaatgaag gctgatgacc
     2941 gcgcttccgg ttgccgatac agcaaccgct ccaaggccag atcgtcacaa tcttccggca
     3001 gcggccaacc gacgctcgcc ccctcggcca gccgcaggta tttggcaacc acgcccttgg
     3061 agatcttcag tgcccgggcg atggcctcaa ggctcagatc agtcgtgtga cggtatcgca
     3121 gtacatctcg aatcttgcgc atcgatagtc tctcctgggg catgctcgct cacttccaaa
     3181 aaggcagcga ggatacccgt aactatccat gcagcatcac atcgatcggt cacgttcagc
     3241 gtgaaatcgc ggtcacgctc tcgtgaaacg ccggtcacgc tttggtgaaa tcaccggtca
     3301 cgatggcgtg aaatacgcac ttcggcgagc atggcggcgc cgttgtcagt catcagggcg
     3361 cgtggcaagc cgcgcttcat gaaggcctgc gacaggccat gcacgagact ctcggcggtc
     3421 tcgtcgagat accactgcag atggcagacc agccgcgagc gatcgtcgat gacgccaaac
     3481 agcagcggct tgacccacag gccggcgcgg gtgaggacct tgcgggagca atgatggaag
     3541 tcgagatgcc aaagggcgct gacgtagtcg acttcgtagc tcctgacttc cagccggtcg
     3601 agccggtcgc gggccagcag cgcgccggcg gtcgtccgtt tgggcgacgc ctggcgaaac
     3661 atcccctgcg tctggaggta acgacggatc gtcggatacg acgccagcga cgcgtcggtc
     3721 ccagccaggg cgacccgcag attgtcgaag tgcagttgca cggtccagcc cggatgctcg
     3781 tgatactgcg ccgtcaggat ctggatgacc acgggggaga ggctcttgaa gcgaccgagg
     3841 ttgctgcgca ggcgattgcg cagactggcc accggatcgg tggccttccg ggccgcgtag
     3901 taccagcgct cgatggtcga ggcgccaaag cagacgtcca gtccggagac cggatgccgc
     3961 caggacttgg tcgacagggc gaggagtgcc ggggataact cgccggcggc gggcggcgcc
     4021 gccagcaagg ggccgatgat cgcaaagcgc aggcgcgccc agcgatcacg tttgagggga
     4081 tcgtgagaag aggtcaagag gggcttccag aacgtcggca ccggcggcac caggacggga
     4141 agggaagcct acaagccctg gcagatgtcg cctatcccca tcctctgcgg gaaatcagcg
     4201 acccccacgc aacgcgctcg gtcggaccgt gatcggcgtc agaaagacga ggagatgccc
     4261 catggcttct gcggcgtcgc cgacaaaacg ttccagcaga ctggtcggaa agcgctccgt
     4321 caccggcggc ataaagcgcg cgccagaggc ttgccagagc gtgctcagcg ggaactggtc
     4381 tttccaccat tgccgccaac gttccagggt acgcaaggga atcgccagtg tcgccgacag
     4441 cctcgccgcc ccggggttct gcccggcatg ccgggcagaa ccgagcacca ccagcagcgc
     4501 cagatagacg cgccggccca ggaaacgtac cgacatcgag gtcgtccgac gacgacaatc
     4561 gctgcagcag aagctgaacc gccgagcaaa gtcggcgtac cgttccggcg ggcacccccg
     4621 cggcttgcgt gggtagtcgg cacggtgcag aacaccgccg caggcgcagc ctcccgctcg
     4681 cgtttcgccc gccaactccg cgtcaacgcg cagcaaccag ctaaaaaaat gaggatcctg
     4741 caatagggca tggcacactg gcatggtctc ttggagcgca aaccagagat tcccccaaag
     4801 ggcgcgtttg ttcaagctcc ccgagggggg tcacccccgc aacatcaccc ggcttgatca
     4861 ggcaggcagg gaagtccatc acggacgtgg accgtgctca gctgagcgcc ggtgcgtccc
     4921 gggcagcggt cgccatcggc cagttcactg ttgtccgagg atctcctcga gcatccggtc
     4981 ta
//
LOCUS       DAOSIT010000114         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15067_length_4980_cov_9.463553, whole genome shotgun sequence.
ACCESSION   DAOSIT010000114 DAOSIT010000000
VERSION     DAOSIT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15067_length_4980_cov_9.463553"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="mnmE"
                     /locus_tag="PLL14_04040"
     CDS             complement(<1..1274)
                     /gene="mnmE"
                     /locus_tag="PLL14_04040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG03124.1"
                     /translation="MAEKPPTTARPESGRDTIAAIATAAGRAGIGIVRISGANLGEFA
                     VALGGRRPAARVATLSDFRAVDGSTIDTGILLYFPAPHSFTGEDVLELHGHGGSVVMQ
                     MLLARSLELGARLADPGEFTRRAYLNGKIDLAQAEAVIDLIDASTIAAARGAVRSLQG
                     EFSREVKALLAQLIELRMLVEATLDFPDEELDFLESTDAFGRLDRLQQRLTLVFDRAQ
                     QGHLLQGGLNVVLAGQPNVGKSSLLNRLAGDELAIVTPLPGTTRDLVRSILQVEGIPL
                     HVIDTAGLRATDDQIEQMGIERTWREIERADVVVLLVDARVGLGDADRAILSQLPAHL
                     ARLTVYNKIDLAAPPVDSQPTGDVAAIALSAKTGEGVDLLRRELLRIVGWHPAEDVFI
                     ARERHLRALAETRRCIEVGRERLPQLELFAEEL"
     gene            complement(1240..>1899)
                     /gene="yidC"
                     /locus_tag="PLL14_04045"
     CDS             complement(1240..>1899)
                     /gene="yidC"
                     /locus_tag="PLL14_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG03125.1"
                     /translation="VDYGWLTIIAAPIFWCLEAIYKVVGNWGWAIVLLTVGIKLLFFP
                     LSAASYKSMAKMRTVTPRLMAMKERYGDDRQKLNQEMMALYKREKINPLGGCLPIAVQ
                     IPVFIALYWALLGAVEIRDAPWILWITDLSAADPYYVMPVIMVVTMLIQTKLNPTPPD
                     PIQAKVMMMMPFIFGAMFFFFPAGLVLYWIVNNILSIAQQWQITRMIESGGKAANDST
                     S"
     assembly_gap    1900..1999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2000..2319)
                     /gene="yidC"
                     /locus_tag="PLL14_04050"
     CDS             complement(<2000..2319)
                     /gene="yidC"
                     /locus_tag="PLL14_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOG03126.1"
                     /translation="MDNRRLLLLIIFSFSMVMLWDAWQKYSQPKAILPAPVATTAVPP
                     APQPSANLHEPRPAAPGTAVATTVETTERVTVKTDLFVAEISKQGGDIVRLEFNAYKD
                     SGNKA"
     gene            complement(2346..2555)
                     /gene="yidD"
                     /locus_tag="PLL14_04055"
     CDS             complement(2346..2555)
                     /gene="yidD"
                     /locus_tag="PLL14_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG03127.1"
                     /translation="MKHLLLALIGLYRYAVSPMLGRRCRFFPSCSEYAAEAVERHGAG
                     KGTALALHRLCRCHPWNAGGFDPVP"
     gene            complement(2560..2958)
                     /gene="rnpA"
                     /locus_tag="PLL14_04060"
     CDS             complement(2560..2958)
                     /gene="rnpA"
                     /locus_tag="PLL14_04060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG03128.1"
                     /translation="MSEQPSVIGLRAVSAAAFPKRYRLIKTDEYSSVFGFRRVLKSRH
                     FLLHYRPRSPEEVPGARLGLVVAKRFLRRSVDRNLVRRLAREQFRLMRACLRSSDFVL
                     RLAVRPPVLDRQAMAQEIRGLLSKAALPPR"
     gene            complement(2955..3089)
                     /gene="rpmH"
                     /locus_tag="PLL14_04065"
     CDS             complement(2955..3089)
                     /gene="rpmH"
                     /locus_tag="PLL14_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG03129.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMATRGGRAVIRARRAKGRHRLAV
                     "
     gene            3391..4785
                     /gene="dnaA"
                     /locus_tag="PLL14_04070"
     CDS             3391..4785
                     /gene="dnaA"
                     /locus_tag="PLL14_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG03130.1"
                     /translation="MSSFWASCLSQFEQELPPQQFNTWIRPLRLEGEDDFAHGLRLLA
                     PNGFILKWVKERYLTRIEALGSEFFAVPVNISLTLGTRAAVPSSATADPPGKAIPTRA
                     ATAAATTAKNTSALERERSNYEKTRLIPTFTFDGLVVGKGNDLARAAARQVSISPGEA
                     TYNPLFIYGGAGLGKTHLIHAIGNQILEHHPDKVVRYVHAEDYYSDVVRAYQQKSFDV
                     FKRYYRSLDVLLLDDVHFFNGKNRTQEEFFYVFNSLSESRKQIVISSDTYPKNISGLE
                     DRLITRFDWGLTVQIEPPETEMRVAILKKKAEMEGVALNDEVAFYVAKHLRSNVRELE
                     GALKKVLAYSAFHGQQISLDLAKEALKDVIGSVNRQLTVENIQKTVAEYFKIKVADLF
                     SKKRTRVIVRPRQIAMWLAKNLTSQSYPSIGDAFGGRDHTTVLHAVRTIDELRARDND
                     LNHDVHVLLQVLKG"
BASE COUNT         1108 a         1395 c         1413 g          964 t
ORIGIN      
        1 agttcctcgg caaacagttc cagttgcggc aagcgctcgc gcccgacctc aatgcagcgg
       61 cgtgtttcgg caagcgcgcg caggtggcgt tcgcgggcga tgaaaacgtc ttcggcagga
      121 tgccaaccca caatgcgcaa caactcccgg cgcagcagat ccacaccctc accggtcttg
      181 gcggaaaggg caattgcggc tacatcgccg gtcggctggc tgtcgacggg cggcgctgcg
      241 aggtcgatct tgttgtacac ggtgagccgt gcaagatggg ctggcagttg cgaaaggatg
      301 gcccgatcgg catcgccaag gccgacgcgg gcgtccacca gcaggacgac cacatcggcg
      361 cgctcgattt cgcgccaggt acgttcgatt cccatctgct cgatctgatc gtcggtggca
      421 cgcaggcccg cggtgtcgat cacgtgcagc ggtatgcctt cgacctgcag tatgctgcgc
      481 accagatcgc gggtcgtgcc ggggagcggc gtgacgatgg ctagttcgtc cccggcgagg
      541 cgattgagca ggctggactt gccgacattg ggctggccgg ccagcaccac attcaagcca
      601 ccctgcaaca gatggccttg ttgcgcgcgg tcgaaaacca gggtcaaacg ctgctgcaag
      661 cggtcgagac gcccgaaggc atcggtcgat tcgagaaagt ccagctcctc gtccgggaaa
      721 tccagggtcg cctccaccag catccgcagt tcgatcaact gtgccagcag cgctttcact
      781 tccctggaaa attcaccctg cagggagcgt acggcgccac gcgcggcggc gatggtcgag
      841 gcgtcgatga ggtcgatgac cgcctcggcc tgtgccaggt cgatcttgcc gttgagatag
      901 gcccggcggg tgaactctcc cgggtcggcc agcctggcgc caagttcgag gctgcgtgca
      961 agcagcattt gcatgaccac cgatccgcca tgtccatgca gttcgagcac gtcctcaccg
     1021 gtgaaggaat gcggcgcggg aaaatacagc aggatgccgg tgtcgatggt gctgccatcg
     1081 acggcccgga agtcgctgag cgtggcaacg cgcgctgcgg gtcgcctgcc gccaagggct
     1141 acggcgaatt cgccgagatt tgcgccggag atgcggacga taccaatgcc ggcgcgaccg
     1201 gccgcggtcg cgatggcggc aatggtatcc cggccggact caggacgtgc tgtcgttggc
     1261 ggcttttccg ccactttcga tcatccgggt gatctgccac tgctgggcga tcgacagaat
     1321 attgttcacg atccagtaga gcaccagacc ggccgggaag aagaagaaca tcgcgccgaa
     1381 aatgaacggc atcatcatca tcaccttggc ctggatggga tcgggcggtg tcggattgag
     1441 cttggtctgg atcagcatcg tcaccaccat gatcaccggc atcacatagt agggatctgc
     1501 cgccgacaga tcggtaatcc agagaatcca cggtgcatcg cgaatttcga cggcccccag
     1561 cagcgcccag tacagggcga tgaatacggg aatctgtacc gcgatcggca ggcaaccacc
     1621 cagcggattg attttctcgc gcttgtaaag cgccatcatc tcctggttga gcttctggcg
     1681 atcgtcaccg tagcgttcct tcatggccat caggcgcggc gtgacggtac gcatcttggc
     1741 catcgacttg tagctcgccg ccgacagcgg aaagaacagc agcttgatgc cgaccgtcaa
     1801 caagacaatc gcccagcccc agttgcctac gaccttgtag atcgcttcca ggcaccagaa
     1861 gatcggcgcc gcgatgatcg tcagccaacc gtagtcgacn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnng ccttgttgcc gctgtccttg taggcattga actcgaggcg
     2041 cacgatgtcg cctccctgct tcgagatctc cgcgacgaac aggtcggtct tgaccgtcac
     2101 tctctcggta gtctccacag tcgtggcgac ggccgttccg ggcgctgccg gacgcggctc
     2161 atgtaaattc gccgaaggct gtggtgcggg tggtaccgct gtcgttgcga cgggtgccgg
     2221 caagattgcc ttcggctggc tgtatttctg ccaggcatcc caaagcatga ccatggaaaa
     2281 agagaagatg atcaggagca gcaggcgtcg attgtccatg aaggcctata cgattcgatt
     2341 gttgttcagg gtacggggtc aaaaccgcca gcattccacg gatgacagcg gcacaaacgg
     2401 tgcagagcaa gggcagtgcc cttgccagca ccatgcctct ctacggcttc ggctgcgtac
     2461 tcggaacagc ttgggaaaaa acggcaacga cggccgagca ttgggctgac cgcatatcgg
     2521 tagagaccta tcaacgccag caacaagtgt ttcatcagct catcgtggag gtaaggcagc
     2581 cttgctcaac aaaccgcgga tctcctgcgc catggcttgc cggtcaagaa cgggtggcct
     2641 gactgccaga cgcagcacga aatcgctcga gcgaagacag gcacgcatca gacggaactg
     2701 ttcacgggcc agacgtcgga ccaggtttcg atcaaccgag cgacgcaaaa agcgcttggc
     2761 gacgaccagg ccaagccgtg ccccaggcac ttcctctggg ctacgagggc ggtaatgtag
     2821 caggaaatgc ctgcttttca gcaccctcct gaaaccaaaa acggatgagt actcatccgt
     2881 tttgatcagg cggtaacgct tcggaaaagc ggcagcggat accgcacgaa gaccgatgac
     2941 agaaggctgt tcgctcaaac ggcgaggcgg tggcgacctt tggcacgacg cgcccggatt
     3001 acggcacgac caccgcgagt cgccatgcgg acaaggaagc cgtgagtacg cttgcggcgc
     3061 acaaccgacg gttgataggt acgtttcatg gttaagccct tgaagtcaaa gcaagataaa
     3121 catgcaatta gacaacctca ggcggatcct tgtcaaggcc atatttttgc tcaagctgtg
     3181 gataactttc gggcaacggg ttagaatttg tgcttcactg cctttggaat gacgaccctg
     3241 ggcggcaggt cgaacggttg tcaccgcaag gtcgcaatct ttcggtgacc ggaaggaccg
     3301 tgacaaggca acatatgtca ctgctttaaa aaattaaagt caaatatatt ccccgcgaca
     3361 tcacgatcgg actgcacgct atgactgtcg atgagtagtt tttgggcctc ctgtttaagc
     3421 cagttcgaac aggaactccc gccacagcaa ttcaatacct ggattcgccc tcttcgcctt
     3481 gaaggcgagg atgattttgc ccacggttta cgtctgctcg cgccgaacgg cttcatcctg
     3541 aagtgggtca aggaacgcta cctgacacga attgaagctc tcgggagcga attctttgcc
     3601 gttccggtaa acatcagcct gactttggga acgcgcgcag ctgtcccgtc aagcgcaact
     3661 gcggacccac ccggcaaagc aatacccact cgggcggcaa cggctgccgc cacgaccgcc
     3721 aaaaacacgt cggcgctcga aagggagcgg tcgaactacg aaaagacacg cctgattccg
     3781 acctttacct tcgatggact ggtggtcggt aaaggcaacg acctggcccg cgccgctgcg
     3841 cggcaggttt cgatcagccc tggtgaagca acctacaacc ccttgttcat ctacgggggt
     3901 gccggactcg gcaagacaca cctgatacac gccatcggca accagatcct ggagcatcat
     3961 ccggacaagg tggtgcgcta tgtacatgcc gaggactact attcggatgt cgttcgtgct
     4021 taccagcaga agtcatttga cgtattcaag cgctactatc gttcactgga tgtcctgcta
     4081 ctcgacgacg tacatttttt taacggcaag aaccggacac aggaagaatt tttctatgtt
     4141 ttcaattccc tgagtgagtc cagaaaacag attgtgatca gcagcgacac ctatccgaaa
     4201 aacatctccg gccttgagga tcggctgatc acgcgctttg actggggtct gacggttcag
     4261 atcgaaccgc cggaaacgga aatgcgagtc gcgatcttga agaagaaagc cgagatggag
     4321 ggggtggcgc tcaatgacga ggttgccttc tacgttgcca agcacctgcg atcgaacgtt
     4381 cgtgaacttg agggggcact caagaaagtg cttgcctact ctgcatttca tgggcagcag
     4441 atctcgcttg atctggccaa ggaagcactc aaggacgtca tcggttcggt caaccgacaa
     4501 cttacggtcg aaaacatcca gaagacagta gctgagtatt tcaagatcaa agttgccgac
     4561 ctgttctcca aaaagagaac acgggtaatc gttcgcccac gtcagatcgc catgtggttg
     4621 gcaaaaaatc tcacttcgca gagctacccc tcgatcggtg atgcctttgg tggccgtgat
     4681 catacgaccg tcctgcacgc cgtgcgcacc atagacgagc tacgtgccag agataacgac
     4741 ctcaaccacg acgtgcacgt tcttctgcag gttctgaagg gctaggaaac tggtggggaa
     4801 agccgtggat aagtcactga caaatagtga acaaagtcga tggggcggga ctgtggataa
     4861 cttgtgagtt tttgcccaca gcttcatacc gcctttctgc agctttgcat ttacacatta
     4921 actaattgaa ttaaaaaaga aattacaaga tatcaacaga tttgaccaga ctatagtctt
//
LOCUS       DAOSIT010000115         4956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15158_length_4956_cov_9.785758, whole genome shotgun sequence.
ACCESSION   DAOSIT010000115 DAOSIT010000000
VERSION     DAOSIT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15158_length_4956_cov_9.785758"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLL14_04075"
     CDS             <1..444
                     /locus_tag="PLL14_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG03131.1"
                     /translation="RPAGRIGQWVFDRGALCGTAGVMAFVLSGHGAWETLDNAALANA
                     LQRELADTLRTRLPPALWYQVIRERRATFSCRPDLRRPAAATARNGLWLAGDYVCAEY
                     PATLEAAVRSGVAAARGLLLSAPPPTARRASAGDGNSAPRSLHHG"
     gene            complement(365..1051)
                     /locus_tag="PLL14_04080"
     CDS             complement(365..1051)
                     /locus_tag="PLL14_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG03132.1"
                     /translation="MVRDASMVVEVRDLAKRVDNGGEPLTILHEISFAVAAGETVAIV
                     GASGSGKSTLLGLLAGLDLPSSGSVALAGEALGALDEDARAVLRGRLLGFVFQTFQLL
                     PSLNAVENVMLPLELAGTANARAEAEYWLQRVGLAHRLRHYPKHLSGGEQQRVALARA
                     FAPQPRLVLADEPTGNLDAATGHQVIELMFAINAERGTTLILVTHDEEIAARCSRRLR
                     MHAGQLEEVR"
     gene            1050..1667
                     /locus_tag="PLL14_04085"
     CDS             1050..1667
                     /locus_tag="PLL14_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HOG03133.1"
                     /translation="MFSFPNYRRLLLAGLMLLAPLAQAAQTILIFGDSLSAGYGIRQD
                     AAWPALLVKRLQEKRLDYNVVNASISGETSSGGRTRIDAALARHSPQVVVVALGANDG
                     LRGLPVAQLRDNLTAIVASAQKKRARVLLVGQRIPPNYGAYAQQFHAVFGEVARARKT
                     ALVDFLLDGVATQSALFQADNLHPTAEAQTLLLDNVWQGLAPLLK"
     gene            1696..>1898
                     /locus_tag="PLL14_04090"
     CDS             1696..>1898
                     /locus_tag="PLL14_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG03134.1"
                     /translation="MNHDIVSVERISDLSAFDEIIDVRSPTEFAEDHIPGSINCPVLD
                     DRQRIEIGTLYKQVSPFEARKLGA"
     assembly_gap    1899..1998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1999..2266
                     /locus_tag="PLL14_04095"
     CDS             <1999..2266
                     /locus_tag="PLL14_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOG03135.1"
                     /translation="LNAPEWLKARLQPLRNLQTTETIVRWGEYVHDGLWRELVSELLQ
                     LHYDPLYSRSQQQNYAGFSAPLTLSSDDLTPAGIEALARRVCAG"
     gene            2351..2794
                     /locus_tag="PLL14_04100"
     CDS             2351..2794
                     /locus_tag="PLL14_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOG03136.1"
                     /translation="MAQDKHPVVVSENGLGPYQRTVHVGRHTLLADEPVSLGGNDAGP
                     EPFDFLLASLGACTSITLRMYAERKQLPLTGIRVELLHDQVDSEKQGTVDRIVRTIIL
                     EGELTAAQRAGLLAIANKCPMYRTLQSDLRIDSLLADRSAAETAE"
     gene            2945..>4956
                     /locus_tag="PLL14_04105"
     CDS             2945..>4956
                     /locus_tag="PLL14_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOG03137.1"
                     /translation="MLEHHYLTTLFEPKSVAVIGASDRENSVGNVLFRNILDSGYKGR
                     LYPINTAHDTIQGVQAYKSIEEISARVELAVVVTRPQTVPKIIEQCGRSGVKNAIVIT
                     SGFAEAGHSGAALERKMMEIARSYGVRLLGPNCLGIIRPNLGLNATFARIHANAGNLA
                     LVSQSGAICSAVLDWATSNRIGFSSVISLGGTADVDFGEILDYLIYDNLTHYILLYVE
                     GIRDARRFMSALRSAARIKPIVLLKAGRHAGGLAAVQTHSGMPGGSDIVFDAAVRRAG
                     VVRVKNIGQLFYAAKALASKFRPQGKRLAIITNGGGPGAMAADRAGDLEIPLAELSAS
                     TIQALNAAMPPTWSQRNPVDIEGDATPKRYHDAILAVAADNAVDGILVMLSPQAMTQP
                     LEVAKAVIEVDLLTAMPILTCWMGEEQVYEARKLLEDAGIPSFRMPETAIELYYHIST
                     YYWNQKLLLQTPAPVSKHSRPETEGAKMLIEAVLHERRKLLSEMESKAILRAFRIPVA
                     QTMVAHTPTESLLLAEQIGFPIAMKIDSPDIVHKSEVGGVRLNITNAPAIRNAYHDII
                     ETVKKRHPTARINGVSIEPYLARPNGRELMIGVSRDPVFGPIITFGAGGIEVEVFSDR
                     AVALPPLNRFLARDLIQSTRASKLLGEFRNMPPVNLEALEDVLLHVS"
BASE COUNT          912 a         1653 c         1479 g          812 t
ORIGIN      
        1 cgccctgccg gtcggatcgg gcaatgggtg ttcgaccgtg gcgcactctg cggtacggcg
       61 ggagtcatgg ccttcgtgct cagcggccat ggcgcctggg aaacgcttga caatgccgcg
      121 ctggccaacg cactgcagcg cgaactcgcc gacacactgc gcacccggct gccgccggcg
      181 ctctggtatc aggtcattcg cgaacgtcgc gcgacgtttt cctgccgccc cgatctgcgc
      241 cggccagccg ccgcgaccgc gcgcaacggt ttatggctgg cgggtgacta cgtctgcgcc
      301 gagtatccgg caacgctcga agccgcggtg cgcagcggcg tcgccgcggc acgcgggctc
      361 ctgctcagcg cacctcctcc aactgcccgg cgtgcatccg caggcgacgg gaacagcgcg
      421 ccgcgatctc ttcatcatgg gtgacgagaa tcagcgtcgt gccgcgttcg gcgttgatcg
      481 caaacatcaa ctcgatgacc tgatgaccgg tcgccgcgtc gagattgccg gtcggctcgt
      541 cggccagcac caggcgaggt tgcggagcaa aggcacgcgc cagcgcgacc cgctgctgct
      601 cgccgcccga gaggtgtttc ggatagtgcc gcaggcggtg tgccagcccg acccgttgca
      661 gccagtactc ggcttcggcg cgcgcgttgg ccgtgccggc cagttccagc ggcaacatca
      721 cgttctcgac cgcgttcagc gagggcaaca gttgaaaggt ctggaagaca aaacccagca
      781 ggcggccgcg caagacggcg cgcgcgtctt cgtcgagcgc gcccagcgct tcgccggcca
      841 gcgcgaccga gccgctgctc ggcaggtcga ggccggccag caggccgagc agggttgatt
      901 tcccggaacc cgaagcgccg acgatcgcca ccgtctcgcc ggccgcgacg gcgaacgaga
      961 tctcgtgcag aatggtcaac ggctcgccgc cgttatccac ccgcttggcc aaatcccgaa
     1021 cctcaacgac catcgaagcg tcccggacca tgttcagttt ccccaattac cgtcggctgc
     1081 tgcttgccgg actgatgttg ctcgcgccac tggcgcaggc cgcccagacc atcctgatct
     1141 tcggcgattc gctttccgcc ggctacggca ttcgccagga cgccgcctgg cccgccttgc
     1201 tggtcaaacg cctgcaggag aagaggctgg attataacgt cgtcaatgcc agcatctcgg
     1261 gagaaaccag cagtggtggc cgtacccgca tcgatgcggc gctggccagg cactcgccac
     1321 aggtggtggt ggtcgccctc ggtgccaacg acggcttgcg gggattgccg gtggcgcagt
     1381 tgcgcgacaa cctgacggcg atcgtcgcca gcgcccagaa gaagcgcgcc cgggtattgc
     1441 tggtcggtca gcgcataccg ccgaactatg gcgcctatgc gcagcagttc cacgcagtct
     1501 tcggcgaagt ggccagggcc cgcaagaccg cgctggtcga tttcctgctc gacggcgtgg
     1561 cgacgcagtc ggcattgttt caggcggaca acctgcatcc gaccgccgag gcgcaaacgc
     1621 tgctgctgga caatgtctgg caggggctcg cgccactgct gaaatgaact gccctgggca
     1681 aaggaccatc cgcgcatgaa ccacgatatc gtcagcgtcg agcgaatcag cgacctgtcc
     1741 gctttcgacg agatcatcga cgtccgctcg ccgacggagt ttgccgagga ccacatcccc
     1801 ggctcgatca actgtccggt gctcgacgac cggcagcgca tcgagatcgg gaccctgtac
     1861 aagcaggtct cccccttcga agccaggaag cttggcgcnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnncc tgaacgcacc cgagtggctc aaggcacgcc tgcagcccct
     2041 gcgcaatctg cagacgaccg agacgatcgt gcgctggggc gagtatgtcc acgacggctt
     2101 gtggcgcgaa ctggtcagcg agttgctgca actgcactat gacccgctct acagccgctc
     2161 gcagcagcag aactacgccg gattcagcgc gccgctgacg ctgagcagcg acgatctgac
     2221 gccggccgga atcgaagccc tggcgcgccg ggtttgcgcc ggctagtcag cgccaggctc
     2281 cggtcaaaag aaatcgtcat cgcggtgcgt ccttccttta tcatgctcca ggtcggctgg
     2341 agttgagcac atggcacaag acaaacaccc tgtcgtcgtc agcgagaacg ggctgggacc
     2401 ttaccagcgg acggtgcatg tcggcaggca taccctgctt gccgacgaac cggtgtccct
     2461 gggcggcaac gatgccggcc cggaaccgtt cgacttcctg ctcgccagcc tcggggcctg
     2521 cacctcgatc acgctgcgca tgtacgcgga acgcaagcaa ctgccgctga ctggaatccg
     2581 cgtggaactg ctgcacgacc aggtcgacag cgagaagcag ggcacagtcg atcgcatcgt
     2641 gcgcacgatc atcctcgaag gcgagctgac ggccgcgcag cgcgccgggc tgctggcaat
     2701 cgccaacaaa tgtccgatgt accgcaccct gcaatccgac ctcaggatcg acagtttact
     2761 ggccgaccgc agtgcggcgg aaaccgccga gtaagtcatg ccatcaccct tgcggcgcgg
     2821 caaatgtcac gacacggcgc gattagagcc cgctctctca agacttggcg tgccatctgt
     2881 ggtaaattga tgcagtgcaa caacaacccg tacggcgcct ctccaacaag aggtgaaccc
     2941 ccccatgtta gaacatcact acctcaccac actttttgaa cccaagtccg tcgccgtcat
     3001 cggcgcgtcg gaccgggaaa attcggtcgg caacgtcctc ttcaggaaca tcctggattc
     3061 gggctacaag ggccgcctgt atccgatcaa caccgcccac gacaccatcc agggcgtgca
     3121 ggcctacaag tcgattgaag agatcagcgc gcgcgtcgag ctggcggtcg tcgtcaccag
     3181 accacagacg gtccccaaga tcatcgagca gtgcggtcgc agcggcgtaa agaacgccat
     3241 cgtgatcact tccggttttg ccgaagccgg ccactcgggc gccgcgctgg agcgcaagat
     3301 gatggagatt gcgcgcagct acggcgttcg cctgctgggc ccaaactgcc tgggcatcat
     3361 ccgccccaat ctcggactca acgcgacttt tgcccgcatc cacgccaatg ccggcaatct
     3421 ggcgctggtt tcgcaatcgg gagcgatctg ctcggccgtc ctcgactggg cgaccagcaa
     3481 ccgcatcggc ttttccagcg tcatctcgct gggtggcacc gccgatgtcg atttcgggga
     3541 aatcctcgat tacctgattt acgacaacct cacgcattac atcctgcttt acgtcgaagg
     3601 cattcgtgac gcccggcgct tcatgagcgc gctgcgttcg gccgcgcgca tcaagccgat
     3661 cgtcctgttg aaagccggtc gccacgccgg cgggctggcg gcagtgcaaa cgcactcggg
     3721 aatgcccggg ggttccgaca tcgtcttcga cgccgcggtc cgccgcgccg gcgtcgtacg
     3781 cgtcaagaac atcggccagt tgttctatgc ggcgaaagcc ctggcctcga agttccgtcc
     3841 gcaaggcaag cgcctggcga tcatcaccaa cggcggcgga ccgggtgcca tggccgcaga
     3901 tcgcgccgga gacctcgaga tcccgctcgc cgaattgtcg gcaagcacca tccaggcgct
     3961 caatgcggcg atgccaccga cctggtcgca acgcaacccg gtagacatcg agggggacgc
     4021 caccccgaag cgctatcacg acgccattct cgccgtcgcc gccgacaacg ccgtcgatgg
     4081 cattctggtg atgctctcgc cgcaggcgat gacccaaccg ctggaagtcg ccaaggcggt
     4141 gatcgaagtc gacctgttga cggccatgcc gattctcacc tgctggatgg gcgaggaaca
     4201 ggtctacgag gcacgcaagc tgctcgagga tgccggcatt ccctccttcc ggatgccgga
     4261 aacggccatc gaactgtact accacatctc gacctactac tggaaccaga aactcctgct
     4321 gcagaccccg gcaccagtat cgaagcattc gcgtccggag accgagggcg ccaagatgtt
     4381 gatcgaggcg gtcctgcacg agcgccgcaa gctgctctcc gaaatggagt cgaaggccat
     4441 cctgcgcgcg ttccggattc cggtggcaca gaccatggtc gcccatacgc cgaccgaatc
     4501 gctgctgctt gccgaacaga tcggtttccc gatcgccatg aagatcgatt cgcccgacat
     4561 cgtccacaag agcgaggtcg gcggtgtacg actgaacatc accaacgctc cggcgattcg
     4621 caacgcctat cacgacatca tcgagacggt caagaagcgg cacccgacgg cacgcatcaa
     4681 cggcgtctcg atcgaaccct acctggcacg gccgaacggt cgcgagttga tgatcggcgt
     4741 ctcgcgagat ccggtgttcg gcccgatcat cactttcggc gccggcggca tcgaagtgga
     4801 ggtcttcagc gatcgcgctg tcgccctgcc gccactcaac cgcttcctgg cgcgcgacct
     4861 gatccagtcg acacgcgcct cgaagctgct cggcgagttc cgcaacatgc cgccggtcaa
     4921 cctcgaagcg ctcgaagacg tgctgctgca cgtttc
//
LOCUS       DAOSIT010000116         4933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15251_length_4933_cov_9.532185, whole genome shotgun sequence.
ACCESSION   DAOSIT010000116 DAOSIT010000000
VERSION     DAOSIT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15251_length_4933_cov_9.532185"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="pgi"
                     /locus_tag="PLL14_04110"
     CDS             <1..431
                     /gene="pgi"
                     /locus_tag="PLL14_04110"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766610.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG03138.1"
                     /translation="VRSDYPLPGHQEALLANCFAQSAALAFGKTAAEARSELSRSMSA
                     EALEALLPHKVFPGNQPSSTLLLSRLDPQTLGALIALYEHKVFVQGVIWGLNSFDQWG
                     VELGKAVASSILPALDNPVLAEQFDASTRGLLNFSRQHLA"
     gene            492..2129
                     /locus_tag="PLL14_04115"
     CDS             492..2129
                     /locus_tag="PLL14_04115"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HOG03139.1"
                     /translation="MQVISVPSAPFAGQKPGTSGLRKKVTVFRQERYLENFVQSIFDT
                     LSGQQGQTLVLGGDGRYYNDVAIQTILRMAAAAGFGRVLVGRDGILSTPAASAVIRKW
                     RAFGGIILSASHNAGGPDGDFGIKYNLGNGGPANEAFTDAVYRRTLEISAYNSIDAPD
                     IDLSRLGELRVGDMIVNIIDPVADYAELLESLFDFERIAALLARPDFRMRFDAMHAVT
                     GPYARAILEDRLGAPAGTVVNGTPLPDFGGGHPDPNPVYAAELVAALADGSSGLSFGA
                     ASDGDGDRNMIVGRGFVVSPSDSLAVLAANHALIPAYAGGLAGVARSMPTSCAVDRVA
                     AALGIPCYETPTGWKYFGSLLDAGKATLCGEESAGTGSDHVREKDGLWAVLYWLNILA
                     VRNLPADVIVREHWQRFGRNVYSRHDYEGIDTAKGDRLINELRSRLPQLPGSEFAGLR
                     IAAADDFAYTDPVDGSRSERQGIRILLDEGSRAVFRLSGTGTEGATLRVYLERYVADP
                     DRHEVPTQEALAPLIGLAEAVAQIAAITGRSEPDVIS"
     gene            2194..3462
                     /gene="glgC"
                     /locus_tag="PLL14_04120"
     CDS             2194..3462
                     /gene="glgC"
                     /locus_tag="PLL14_04120"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG03140.1"
                     /translation="MVDRKTFQLPRKAIALVLAGGRGSRLHELTDRRAKPAVHFGGKF
                     RIIDFALSNCVNAQIRRVGVVTQYKSHSLLRHLQRGWNFLHGEVNEFVDLLPAQQRID
                     EESWYRGTADAVYQNIDILETYSPAPDFVVILAGDHVYKMNYAVMLVDHVESGAECTV
                     ACIEVPRHEASGFGVMAVDESGMITDFVEKPADPPAMPGRPDMALCSMGVYVFNAKYL
                     YAELARDIVDPTSDHDFGKDIIPRAVANRCAVAHPFSRSCIVAPDEQRKEHYWRDVGT
                     IDAYWDANIDLTATVPALNLYDRNWPVWTYQQQLPPAKFVHNHLDRRGNAIESTVSSG
                     CIVSGEVNRSLLFSSCRVHSYARVNLSVLLPDTTVGHRARLTRCVVDSDCDIPPGMVV
                     GEDPVEDARRFRHTENGVTLITRKMLDRLT"
     gene            3479..4927
                     /gene="glgA"
                     /locus_tag="PLL14_04125"
     CDS             3479..4927
                     /gene="glgA"
                     /locus_tag="PLL14_04125"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOG03141.1"
                     /translation="MALRILHVAAEIYPLLKTGGLADVVAALPAALAARGLDTRILLP
                     GLPAVLNGLVDLRRLIRFGPAFGAAIVTLRLGRLPESGLLAYVIDAPFLYRRDGNPYL
                     GPDGRDWSDNHRRFALLGWVAAHLATGELDRHWQPEVVHAHDWHAGLAPTYIAQNPAL
                     NTATVFTIHNLAFRGLFPLDHHIDLGLLISGAAQSALEFHGQLSFMKSALVHAKRVNT
                     VSPSYAREICTPEFGWGLEGLLRDRGSHLSGILNGVDYSVWDPVTDTALPASYSADHL
                     HGKEVCKHKLQSTLGFAQRSQAPLFAVISRLTAQKGMDLVLGALPALRAEGAQLVVIG
                     SGEAEIEAGFRAAAAAYPDTISVHLGYDEALSHRILAGSDILLVPSRFEPCGLTQLYA
                     LRYGTLPMVRRVGGLADTVIDATAENIKLGTANGFVFNDARSRALAARITEACAQFHE
                     HALWRKIQRRGMLQDFSWADSAVRYEALYRSI"
BASE COUNT          872 a         1512 c         1538 g         1011 t
ORIGIN      
        1 ccgtccgttc cgactatcca ttacccggcc accaggaggc gctgctcgcc aattgttttg
       61 cgcagagcgc agcgctggct tttggcaaga cggcggcgga ggcacgcagc gagttgagtc
      121 gcagcatgtc ggcagaggcg ctcgaggcct tgctgccgca caaggtcttt cccggcaacc
      181 agccgtcatc aacgctgctg ctttcccgcc tcgatccgca gacgctcggc gcgctgatcg
      241 cactctacga gcacaaggta ttcgtccagg gcgtgatctg gggactcaac tcattcgacc
      301 aatggggtgt cgagctcggc aaggcggtgg ccagcagtat tctgccagcc ctcgacaatc
      361 ccgtgctggc cgaacagttc gacgcgtcga cgcggggatt gttgaatttc agccgacaac
      421 atctggcatg aacggattct tgtccggaat cgattcaagg catttatttt gcaacgaggc
      481 aaaacatgac tatgcaggtg atcagcgttc cgagcgcccc tttcgccggc cagaagccgg
      541 ggacgtccgg gctgcggaag aaagtgaccg tattccgcca ggagcgttat ctcgagaatt
      601 tcgtccagtc cattttcgac accctgtccg gccagcaggg acagaccctg gtgcttggcg
      661 gcgatggtcg ctactacaac gatgtggcca tccagaccat cctgcggatg gctgcggcgg
      721 ctggtttcgg gcgtgtgctc gtgggtcgcg acggcattct gtctactcct gcggccagtg
      781 ccgtgatccg caagtggcgg gctttcgggg gcattatcct ttcagccagt cacaatgccg
      841 gtggccctga cggcgatttc gggatcaagt acaacctcgg taacgggggg cccgccaacg
      901 aagcctttac cgatgccgtc tatcggcgca ccctggaaat cagtgcctat aacagcatcg
      961 acgcaccgga tatcgacctg agccgactcg gcgaattgcg tgtcggcgac atgatcgtca
     1021 atatcatcga tccggtggcc gattatgccg aactccttga atcgctgttc gacttcgagc
     1081 gcatcgccgc gctgctggcg cgccccgact ttcgcatgcg ttttgacgcc atgcacgcgg
     1141 ttaccggtcc ctatgcgcgg gcaattctgg aagatcgcct tggtgcacca gccggtacgg
     1201 tggtcaacgg tacgccgctg ccggatttcg gtggcggtca ccccgacccc aaccccgtct
     1261 atgcggccga actggtagcc gccctggccg acggcagttc gggattgtcg ttcggtgcgg
     1321 cttctgacgg tgatggcgac cgcaacatga ttgtcggtcg tggcttcgtc gtcagcccga
     1381 gtgacagcct ggccgttctc gcagccaacc acgcgctgat tccggcttac gccggtgggc
     1441 tggccggtgt cgcgcgttcg atgccgacga gttgcgctgt agaccgcgtg gccgcggcgc
     1501 tcggcatccc ttgctatgaa acaccgaccg gctggaaata cttcgggtcg ctgctggatg
     1561 ctggcaaagc cacgctgtgt ggtgaggaaa gtgcggggac gggttccgat cacgtgcgcg
     1621 agaaggatgg cctgtgggcg gttctctact ggctcaacat tctggccgtc cgcaacctgc
     1681 cagccgacgt gatcgtgcgg gagcactggc agcgtttcgg gcgcaatgtc tactcgcgcc
     1741 acgattacga aggcatcgac acggcgaagg gtgatcgcct gatcaacgaa ctgcgctctc
     1801 gcctgccaca actgccgggt tccgaattcg caggcctgcg cattgccgcg gccgatgatt
     1861 ttgcctatac cgatccggtt gatggctcgc gttcggagcg tcaggggatt cgcattctgc
     1921 tcgatgaggg ttcgcgggcg gtttttcgcc tttcgggcac gggtaccgaa ggggccacgc
     1981 tgcgcgtcta cctagaacgt tatgtcgcag atcccgatcg ccatgaagtt cccacacagg
     2041 aagcactggc gcctttgatc ggtcttgccg aggcggtggc gcagattgca gcgatcaccg
     2101 gccgcagcga gcccgatgtg atcagctagc gagaatgggt gacaccgggt ggcgccacct
     2161 gatgcgatga aacaagctca gaaggataca aagatggttg acagaaagac attccaattg
     2221 ccacgcaagg cgattgccct ggttcttgca ggcggacgcg gcagccgtct gcacgaactc
     2281 acggatcgcc gcgcgaagcc tgcggtgcat tttggcggca aattccggat tatcgatttc
     2341 gccttgtcga actgcgtcaa cgcgcagatt cggcgcgtcg gcgtggtcac gcagtacaag
     2401 tcgcatagcc tcttgcgtca tctgcaacgc ggatggaatt tcctgcatgg cgaagtcaac
     2461 gagtttgtcg acctgcttcc tgcacaacag cgtatcgacg aggaatcctg gtatcgcggt
     2521 acggcggacg cggtttacca gaatatcgat atcctcgaga cctattcgcc ggcaccagat
     2581 ttcgtcgtta ttctcgccgg cgaccacgtc tacaagatga attatgcggt catgctggtc
     2641 gaccatgtcg agagcggtgc cgaatgcacg gtggcgtgca tcgaagtgcc gcgtcatgag
     2701 gccagtggtt tcggggtcat ggcggttgat gagagcggca tgatcacgga ttttgtcgaa
     2761 aaaccggccg acccgccggc gatgccgggc aggcccgata tggccttgtg cagcatgggg
     2821 gtgtacgtgt tcaatgccaa atatctgtac gcggaactgg cccgtgacat cgtggacccg
     2881 acatccgatc acgattttgg caaggacatc attccgcgcg cggtggccaa ccgttgtgcg
     2941 gtggcgcatc ctttctcgcg cagttgcatc gtcgccccgg atgaacagcg caaggagcat
     3001 tactggcgtg acgtcgggac gatcgatgcc tactgggatg cgaacatcga cctgacggcc
     3061 acggtgccgg ccctgaacct ctacgatcgc aactggccgg tatggaccta tcaacagcag
     3121 cttccgcccg ccaagttcgt ccacaaccat ctcgaccggc gcggcaatgc gatcgagtcc
     3181 acggtgtcga gtggttgcat cgtctccggg gaagtcaacc gctcgctgct cttctccagt
     3241 tgccgcgtgc attcgtacgc gcgggtcaat ttgtcggtgc tgctcccgga taccacggtt
     3301 ggtcaccgcg ctcgcctcac ccgttgcgtc gtagacagtg attgcgacat tccaccgggg
     3361 atggtcgtcg gcgaagatcc tgtggaagat gcgcgtcgct tccgtcatac cgaaaacggt
     3421 gtgacgctga tcacgcggaa gatgctcgat cggctgacct gacggatcac gaggcaacat
     3481 cgccttgcgc atcctgcacg tcgccgcgga gatctatccg ctgctcaaga ccggcggcct
     3541 ggccgatgtc gtggcggctc tgcccgccgc cttggccgca cgtggcctgg acacgcgtat
     3601 cttgttgccc gggctgccgg ccgtcctcaa tggactggtc gacctgcggc ggctgatccg
     3661 ctttggtccg gccttcggcg ccgccatcgt cacgctgcgg ctgggccgcc tgccggaaag
     3721 cggcttgctg gcctacgtca tcgatgcgcc ctttctttac cggcgcgacg gcaatcccta
     3781 tctgggtccc gacggccgcg actggagcga caatcatcgg cgctttgcgc tgttgggctg
     3841 ggtcgccgcg cacctggcga ccggtgaact cgaccgacac tggcagccgg aggtggtgca
     3901 cgcgcacgac tggcacgccg gcctggcgcc gacgtatatc gcgcagaatc cggcgctgaa
     3961 cacggccacc gtgtttacca tccacaatct cgctttccgt ggtctctttc cactcgatca
     4021 ccacatcgac cttgggttgc tgatttccgg cgcagcgcaa agcgccctcg aatttcacgg
     4081 gcagttgtcg ttcatgaaat ccgccctcgt gcatgcgaaa cgggtgaaca ccgtcagccc
     4141 cagctacgca cgcgaaatct gcacgccgga gttcggctgg gggctggaag ggctgctccg
     4201 tgatcgcggc agccatcttt ccgggatcct caacggggtc gattattcag tctgggatcc
     4261 cgttaccgac accgcgctcc cagcctccta cagtgccgac catctgcatg gcaaggaagt
     4321 ctgcaagcac aagctgcagt caactctcgg ttttgcgcag cggtcgcagg cgccattgtt
     4381 tgcggtgatc agccgtctga ccgcgcaaaa gggcatggat ctggtcctgg gggcgctgcc
     4441 cgcgctgcgt gcggaaggcg cacaactggt cgtcatcggt agtggcgagg cggagatcga
     4501 ggcgggtttc cgggcggcag cagcggcgta tccggacacc atttccgtgc atctgggtta
     4561 cgatgaggcc ttgtcgcatc gtatcctggc cggatccgac attctgctcg tcccctcgcg
     4621 tttcgagccc tgcggcctga cgcaactcta tgccctgcgc tacgggaccc tgccgatggt
     4681 acggcgggtt ggtggtctcg ccgatacggt gattgatgcc actgcggaga acatcaagct
     4741 gggcaccgcg aacggcttcg tgttcaacga tgccagaagt cgagcactgg ccgctcgcat
     4801 caccgaggcg tgtgcacagt tccacgagca cgcgctgtgg cgcaagatac aaaggcgtgg
     4861 catgctgcag gatttctcat gggccgattc ggccgtgcgc tatgaggcgc tgtaccgcag
     4921 catctgacat ccg
//
LOCUS       DAOSIT010000117         4919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15302_length_4919_cov_8.919408, whole genome shotgun sequence.
ACCESSION   DAOSIT010000117 DAOSIT010000000
VERSION     DAOSIT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15302_length_4919_cov_8.919408"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="PLL14_04130"
     CDS             complement(<1..1949)
                     /locus_tag="PLL14_04130"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOG03142.1"
                     /translation="MSPHPEDDPAYQVFIDSLSYLPPEEIQVVREAYLFSEAAHRGQL
                     RLSGEPYITHPLAVAGAIAEWQMDVEGVVAALLHDVMEDTSVGKVEIAERFGKPVADL
                     VDGLSKLDKIEFQSHEEAQAENFRKMLMAMARDLRVVLIKLADRHHNLQTMAAVRADK
                     RRRIARETLEIYAPIASRLGLNKLFRELQDLSFQLIYPMRARVLARALKAARGNRREL
                     LSRILEGLTGKLDEAGIEARVFGREKGLYSIYRKMLDKHLSFSQVLDVYGFRVLVNDV
                     PTAYLALGALHGLYKPVPGKFKDYIAIPKPNGYQSLHTTLIGPHGTPFELQIRTEAMD
                     NVAQEGVASHWLYKDSEDFGAELQQKTTKWFHSLLDLQSATGDSSEFFEHVKVDLFPH
                     EIYVFTPKGKIIALPRGATVVDLAYAVHTDIGNHCVAAQVNFKLMPLATELTNGDRVE
                     VVTAADAHPSVAWISHVKTGRARSKIRHYLKTAHHDESTALGEKMLNQELRALGVVAS
                     ELPVSSWKNVLRDSGNKLIKEVYTDIGLGFRLAGVVARRLLAREDTPQPADKSPASLV
                     IRGTEGMAVQFAPCCQPIPGDPIIGSIWKGKGLVIHTHDCPAIRKARSAKPTKWLDVE
                     WEPEPGKLFSVRLLIMVQNTVGALGR"
     gene            complement(2042..2248)
                     /gene="rpoZ"
                     /locus_tag="PLL14_04135"
     CDS             complement(2042..2248)
                     /gene="rpoZ"
                     /locus_tag="PLL14_04135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG03143.1"
                     /translation="MARVTVDDCLHRIPNRFQMTLAATYRARQITAGASPLVDPGRDK
                     PTVIALREIAGGRVGLEVLNRGQA"
     gene            complement(2267..2875)
                     /gene="gmk"
                     /locus_tag="PLL14_04140"
     CDS             complement(2267..2875)
                     /gene="gmk"
                     /locus_tag="PLL14_04140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG03144.1"
                     /translation="MSGHLYIVTAPSGAGKTTLVRLLLENDPGICVSISHSTRSPRTG
                     ERDGVHYHFVAVQDFLEKVRKDEFIEWAQVHGNYYGTSRSGIESALQVGQDVLLEIDW
                     QGAQQVRRLFPAAIGVFILPPSLDALERRLCERATDSAATIALRVAAARDEMRHVAEF
                     DYVIINDDLQRALHDLLAVIRAARLRYAAQRRRHSILFATML"
     gene            3033..3389
                     /locus_tag="PLL14_04145"
     CDS             3033..3389
                     /locus_tag="PLL14_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03145.1"
                     /translation="MFRWKLMLHTALPLAALLATNAGAAVPLKILGFDDMSCRAWSAS
                     KDDVEQRALYVAWVRGVLSGHNYANPSQQVSAISSGTVEQHVNRYCTDKPLGQFSDAA
                     FRLSDQFSGRNAAITK"
     gene            3591..4391
                     /gene="map"
                     /locus_tag="PLL14_04150"
     CDS             3591..4391
                     /gene="map"
                     /locus_tag="PLL14_04150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG03146.1"
                     /translation="MSISKKSPQEVEKMRVAGRLTAEVLDYIAPHVRSGVTTGEIDQL
                     CHDYIVNVQGCIPAPLNYAPPGYRPYPKSICASINHQVCHGVPGDRQLKNGDIVNLDV
                     TVISDGYHGDSSRMFQVGETSVQARRLCEVTFECLWLGIAQVRAGAHLGDIGHAIQQH
                     AEQSGYSVVREFCGHGIGAKFHEEPQVLHYGRPNTGIRLESGMVFTIEPMINAGKAAI
                     RTMADGWTIVTKDHSLSAQWEHTILVTETSYEVLTVSEGTRPKPDWIR"
     gene            4391..>4919
                     /locus_tag="PLL14_04155"
     CDS             4391..>4919
                     /locus_tag="PLL14_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOG03147.1"
                     /translation="MTTRTATFDRQALIACCRARLQSGQRTVRERYLCEGDAPRLLHE
                     RCQLIDGILCDLWQALELPSSLALVAVGGYGRGELYPASDVDILLLLPNPPDEALTTS
                     LERAVALFYDIGLEIAPSVRTLDECRRAALDDLTIQTALLEARLLTGNSAVFRALCEQ
                     LMEDLEPQAFFEAKRL"
BASE COUNT          987 a         1568 c         1419 g          945 t
ORIGIN      
        1 cgcccaagag caccaaccgt gttctgtacc atgatcagca aacgcacgct aaacagcttg
       61 ccgggctccg gttcccactc gacgtcaagc cacttcgtcg gtttggcgct acgcgccttg
      121 cggatggccg gacaatcatg ggtatggatg accagaccct tgcccttcca gatcgagccg
      181 atgatcggat cacccgggat cggctggcag cagggcgcga actgcacggc catgccttcg
      241 gtgccacgaa tgaccaggga cgccggcgac ttgtcagccg gctgtggcgt atcctcgcgc
      301 gccagcaggc gacgggcaac caccccggca aggcggaagc ccagcccgat atcggtgtac
      361 acttccttga tcagcttgtt gccggaatct cgcagcacgt tcttccaact cgaaaccggt
      421 agctcagagg caaccacgcc aagcgcccgc aactcctggt tcaacatctt ctcgccaagc
      481 gcggtcgact cgtcatggtg tgccgttttc aggtaatggc ggatcttgct gcgcgccctg
      541 ccagtcttga cgtgactgat ccaggccacg ctcgggtggg catcggcggc agtgaccacc
      601 tcgacccggt ctccgttggt cagttcggtc gccaatggca tgagcttgaa attaacctgc
      661 gcagcaacgc agtggttgcc gatatcggta tgcaccgcat acgccagatc gactaccgtg
      721 gcaccgcgcg gcagggcgat gatcttgcct ttgggcgtga acacgtagat ctcgtgcgga
      781 aagagatcca ccttgacgtg ctcgaagaac tcggacgaat cgccggtcgc actctgcagg
      841 tcgagcagcg agtgaaacca cttggtcgtc ttctgctgca gttcggcacc gaagtcctcg
      901 ctgtccttgt acagccaatg cgaggcaacg ccttcctggg cgacattgtc catcgcctcg
      961 gtgcgaatct gcaactcgaa tggggtaccg tgcggaccga ttagcgtagt atgcagcgac
     1021 tggtagccgt tgggcttggg aatcgcgata tagtccttga acttccctgg caccggttta
     1081 tagaggccgt gcagcgcgcc gagagcgaga taggcggtgg ggacatcatt caccagcact
     1141 cgaaagccgt aaacatcgag cacctgcgag aacgacaggt gcttgtccag catcttgcgg
     1201 tagatcgagt acagcccctt ctcgcgaccg aatacccgcg cctctatgcc cgcctcgtcc
     1261 agcttgcccg tgaggccttc caggattcgc gacagcaact cgcgacggtt gccacgcgct
     1321 gccttgagcg cacgcgccag aacgcgcgcg cgcatcggat aaatcagttg aaacgagaga
     1381 tcctgcaact cgcggaaaag cttgttgagg ccgagccgac tggcgatcgg tgcgtaaatc
     1441 tccagtgtct cgcgggcgat acggcggcgc ttgtcggcgc ggacggcagc catcgtctgc
     1501 aggttgtgat ggcggtcggc cagcttgatc agcaccacac gcaaatcgcg tgccatggcc
     1561 atcagcatct tgcggaagtt ttccgcctgc gcctcctcgt gcgactggaa ctcgatcttg
     1621 tcgagcttcg acagtccatc gaccagatcc gcgaccggtt tgccaaagcg ctcggcaatc
     1681 tcgaccttgc ccaccgacgt gtcctccatc acgtcgtgca gcagcgccgc aaccaccccc
     1741 tccacatcca tctgccattc ggcgatcgca ccggctaccg ccagggggtg agtaatgtag
     1801 ggttctccag acaggcgcag ctgcccacga tgcgcagcct cgctgaacag atacgcctcc
     1861 cttacgacct gaatttcttc gggcggtaga tagctcagac tgtcgataaa aacctgataa
     1921 gcgggatcgt cctctggatg aggcgacagc tgggcattgt cagccaccgg caattcaccg
     1981 gacaggctgg ctgcggcaac cggatccctg atcacacact gcccaaccgg tggggttaaa
     2041 gtcaggcctg accgcggttg aggacttcca gaccgacccg gccgccggcg atttcgcgca
     2101 gcgcaattac cgtcggcttg tcccgacccg ggtcgaccag aggtgatgcg ccagcggtga
     2161 tctgacgggc gcggtaagtg gccgccaagg tcatctgaaa acggttcgga atacggtgca
     2221 gacaatcgtc tacggtcact cttgccatgc aaatctccca ggagagtcag agcattgttg
     2281 cgaacaggat ggaatggcgc cggcgctgcg cagcgtaacg gagtcgtgcg gcgcgaatca
     2341 ccgccaacag gtcgtgcagc gcgcgctgca gatcgtcgtt gataataaca tagtcgaatt
     2401 cggccacatg ccgcatctca tcgcgcgccg cggccacgcg cagagcgatc gttgcggcgc
     2461 tgtcggtggc ccgctcgcaa agacgacgct ccagcgcgtc gagcgagggc ggtagaatga
     2521 acacgccgat cgccgcgggg aacaatcggc gcacctgctg cgccccctgc cagtcaatct
     2581 caagcagcac gtcctggcca acctgcaagg ccgactcgat accgctcctt gatgtgccat
     2641 agtagttgcc atgcacttga gcccattcga tgaactcatc cttacggact ttttccagaa
     2701 aatcctgcac ggcaacgaaa tgatagtgga cgccgtcgcg ttcaccagtt cgcgggctgc
     2761 gcgtgctgtg agaaatggac acacagattc ccggatcgtt ctccagcaac aagcgcacca
     2821 gagtcgtctt cccggcaccg gaaggggccg tgacaatgta gagatgtccg ctcatggttc
     2881 cttcgccagc gaatatatgg cttcaattgt acctgcggcg acatcgattt ttggctctcc
     2941 tagtcgccgg ccgcgccgat gggcatggac tggccccgcg ttacagacga aaattgcgga
     3001 atcgatccgt tcaccgtgcg aggagcccct tgatgttccg atggaagttg atgctgcaca
     3061 ctgccctacc gcttgccgcg ctattggcaa cgaatgccgg cgctgcagtg ccgctcaaga
     3121 ttctcggctt tgacgacatg tcctgccgtg cctggtctgc gtcgaaagac gatgtcgagc
     3181 aacgagcact ctacgtcgcc tgggtacgcg gcgtgctcag tggtcacaac tacgccaacc
     3241 caagccagca ggtttcggcg atttcgagtg gaacggtcga acagcatgtc aaccgctatt
     3301 gcacggacaa accgctgggg cagttcagtg atgcggcttt tcgtcttagc gaccagtttt
     3361 ccggtcgcaa cgccgcgatc accaagtaga aaacgatccg ctcgtgcgac tgtgctgcgt
     3421 cgacctctcc ctggcagagg cctataactc gagagactga cagatcatgc cgtcccgcga
     3481 tggcaactcc tgccgagtcc ggcaggagtg ctttccccag gtggcaaatt gcctaagctc
     3541 cccgcttcct ttatccttcc ccctcctccc cctgccggcg acctgaacgc atgagcatca
     3601 gcaaaaaatc accccaagaa gtcgagaaaa tgcgtgttgc cggccgcctg accgccgagg
     3661 ttctcgacta catcgcgccg catgtcaggt cgggagtcac cactggcgag atcgaccaac
     3721 tctgtcacga ctatatcgtg aatgttcaag gctgcatccc ggcgccgctc aactatgctc
     3781 cacccggcta cagaccttat ccgaagtcga tctgcgcctc gatcaaccat caggtatgtc
     3841 atggcgttcc cggcgaccgt caactcaaga acggcgacat cgtcaatctc gacgtcaccg
     3901 taatcagcga cggttaccac ggcgacagca gccgcatgtt ccaggtcggc gagacgtcgg
     3961 tgcaggcgcg cagactctgc gaggtcactt tcgaatgcct ctggttgggc atcgcacagg
     4021 tcagggcggg cgcccacctc ggtgacatcg ggcatgcaat ccagcaacat gcggagcaat
     4081 cggggtattc ggtggtccgc gagttttgtg ggcacggcat cggcgcgaaa ttccacgaag
     4141 agccgcaagt ccttcactac ggtcgcccaa ataccggcat cagactcgag tccggcatgg
     4201 tgttcaccat cgagccgatg atcaacgccg gcaaagcagc gatccgcacc atggccgacg
     4261 gctggaccat cgtcaccaaa gaccatagcc tgtcggcgca gtgggagcac accatcctcg
     4321 tcaccgaaac gagctacgaa gtcctcaccg tatcggaggg cacacgcccc aagcccgact
     4381 ggatcagata gtgaccacgc gcactgccac tttcgaccgt caggcgctca ttgcctgttg
     4441 tcgggcacga ttgcagtcgg gtcagagaac ggtgcgtgag cgctatctct gcgaaggcga
     4501 cgcaccgcgc ctgttgcacg agcgctgcca actgatcgac ggcatcctct gcgacttgtg
     4561 gcaggccctg gagttgcctt cctcgctggc cttggtcgcg gtcgggggct acggacgcgg
     4621 cgaactgtac ccagcctccg atgtcgatat cctgcttctg ttgccgaatc cacccgacga
     4681 ggccttgacc acctcccttg agcgggcagt ggctctgttc tacgacatcg gcctggagat
     4741 cgcccccagc gtgcgtactc tcgatgagtg caggcgcgcc gccctggacg acctgacgat
     4801 tcagacggcc ttgctcgaag cacgtctgct taccggcaac agcgcggtct ttcgggcact
     4861 ctgtgagcaa ctcatggaag accttgagcc gcaggccttc ttcgaagcca agcgcctgg
//
LOCUS       DAOSIT010000118         4914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15324_length_4914_cov_12.109693, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000118 DAOSIT010000000
VERSION     DAOSIT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15324_length_4914_cov_12.109693"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLL14_04160"
     CDS             <1..366
                     /locus_tag="PLL14_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG03148.1"
                     /translation="FPEYYASDAYFKDPFNEVRGLEPIQRIFAHMFRQVAEPRFVVIE
                     SVVAENGALLVWELHYRRKSSSARGSTRVIRGASHLRFDAHGKVVWHRDYWDAAEELY
                     AGFPGIGWLLRVLKRKLAA"
     gene            535..747
                     /gene="rpsU"
                     /locus_tag="PLL14_04165"
     CDS             535..747
                     /gene="rpsU"
                     /locus_tag="PLL14_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG03149.1"
                     /translation="MPAIRVKENEPFEVAIRRFKRTVEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHHKRMRSQTLPPKLY"
     gene            864..1313
                     /locus_tag="PLL14_04170"
     CDS             864..1313
                     /locus_tag="PLL14_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG03150.1"
                     /translation="MTLKERINEDVKAAMRARAAQTLGALRLLTAAIKQREVDERIAL
                     DDAAIISVIDRMLKQRRDSIAQFAQAGRNDLVDAETFELRLLSAYLPASLSTEELAAA
                     VAKAIAETAATGPGDMGKVMACLRTGLAGRVDMSEASRLVKAQLTGV"
     gene            1356..>2068
                     /gene="dnaG"
                     /locus_tag="PLL14_04175"
     CDS             1356..>2068
                     /gene="dnaG"
                     /locus_tag="PLL14_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG03151.1"
                     /translation="MIPDSFIQEMLYRIDLVDLIDGYVPLKKTGANFAACCPFHSEKS
                     PSFTVSPSKQFYHCFGCGAHGNAIGFLMEYSGLGFVDAIKDLAGRAGMQVPDDHGPAG
                     QHAEKSQALGEIMARAARFYYEQLKGSARAVSYLKERGVSGEIARRFGLGYAPEGWQN
                     LAAAFDDYSSSELQLAGLVINNERGRLYDRFRERVMFPIVSQKGEVIAFGGRVLGQGE
                     PKYLNSPETPLFEKGREVFG"
     assembly_gap    2069..2168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2169..2958
                     /locus_tag="PLL14_04180"
     CDS             <2169..2958
                     /locus_tag="PLL14_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG03152.1"
                     /translation="LTEQKSIAFVFLPEGEDPDSLVRKRGGEALQRLIAQATPLSDFL
                     LRELAGHCDLNSAEGRARLVADAKPLLGRLQSSLLRLQLVKRLAESSGFSQAEVERLC
                     DLQPLARPAPARAPRQAPSLLRPLLRLLLQKPQLAGGLPLDLLPGNSMEARAVRLLCE
                     TIGDGGVRMLAYPALIEKLRGSEYEGILRAAAAELMQQPFAEEGIDAEFEGAVSRLLE
                     GEHKRVFALLQEKVQQLGVSGLSSEEKQQYLTALGARGRPGSVG"
     gene            3011..>4914
                     /gene="rpoD"
                     /locus_tag="PLL14_04185"
     CDS             3011..>4914
                     /gene="rpoD"
                     /locus_tag="PLL14_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOG03153.1"
                     /translation="MVREKAVTTKAAKAKAAADLLAQVGTQPAPVDDETRKMRLKTLI
                     KLGKERGFLTYAEINDHLPDDVVDAEQIESIISTFNDMGIQVYDEAPDAETLLMSDAA
                     PAAGADDADVEEQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIE
                     DGLKHMIQAISACPTTIAEILSCADKIARDEMRIDELIDGLIDPETDGSLEDLTAEVA
                     EEESDDEDEEEEDSAEAVEGAAVAASLLKLKTEGLERLELIRSHYTKAHGVLPRRGSQ
                     DKAYLQLQQQISEEMMGIRFTSKTIERLCDSVRAMVEEARACERKIQRICVDTVRMPR
                     PHFIKVFPGNELNIDWVDAEIAAAGKTYAAILTRNAPNIKEEQRKLLALQDRIGIPLR
                     ELKDINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMK
                     AVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQ
                     ETGLEADPATLAKKMEMPEEKIRKILKISKEPISMETPIGDDDDSHLGDFIEDQATLA
                     PTEAALYSSLRFITKDVLDTLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIR
                     QIEAKALRKLR"
BASE COUNT          976 a         1421 c         1550 g          867 t
ORIGIN      
        1 tttcccgagt actacgcgag tgatgcgtac ttcaaggatc cgttcaacga ggtgcgcggt
       61 ctggagccga tccagcgcat tttcgcgcac atgttccgtc aggttgccga accgcgcttc
      121 gtcgttattg agagtgtggt cgccgagaac ggcgcgctgc tggtttggga actgcattat
      181 cgtcgcaagt cctcgtcggc gcggggttcg acgcgggtga ttcggggtgc gtcgcacctc
      241 aggttcgacg cgcatggcaa ggtcgtctgg catcgcgact actgggatgc cgcggaagag
      301 ctctacgccg ggttcccggg aatcggctgg ctgctgcggg tactgaaaag gaaactcgcg
      361 gcctgagccg gcgggccctt cacgatctcg ccgtgctcgc tacgtcggga cgcggcggca
      421 ccagcgcttc tgcaacgcac ttgtccaaaa acgctctagg aattctgccg aaactgggtt
      481 tataatgcgc ggctaatctg cactgtgata tttttttgaa cttgggaaga gccgatgcct
      541 gccattcgcg tcaaggaaaa tgagccgttc gaagttgcga tccgccgttt caaacgcacc
      601 gttgagaaaa ccggtctgtt gaccgaactg agagctcgcg agttctacga gaagcccacc
      661 gccgagcgca agcgcaagct ggccgccgcg gtcaagcgcc atcacaagcg gatgcgcagc
      721 cagaccctgc ctccaaagct gtattaacac cggtttgccg gtgcctgggc agccgcctgt
      781 ctgaagatgg gcggctgttg gcgtttctgg aacggctgtt gtggtgactt gcgtggcacc
      841 gtcaatcagg aaggagtctc tcgatgaccc tcaaagaacg catcaacgaa gacgtcaagg
      901 cagccatgcg cgcgcgcgcc gcacaaacgc ttggcgcctt gcgtctgctg acggcggcaa
      961 tcaagcagcg ggaggtcgac gagcgcatcg cgctcgatga cgcagcgatc atctccgtca
     1021 tcgacaggat gctcaaacag cgtcgggatt cgatcgcgca atttgcgcag gcggggcgca
     1081 acgatctggt cgatgccgag accttcgagc tgcggttgct gagcgcctat ctccccgcca
     1141 gcctgtcgac ggaggaactt gccgccgcgg tggccaaggc catcgccgag actgcggcca
     1201 ccggccctgg cgacatgggc aaggtgatgg cctgtctgcg cacggggctg gccggccgcg
     1261 tcgacatgag cgaagcgtcg cgcctggtca aagcccaact gactggcgtc tgaccgtctg
     1321 cgccatagcg ctcggctgct ggcaccaggg gctcgatgat cccggactcc ttcatccagg
     1381 aaatgctcta tcgcatcgac ctggtcgacc tgatcgacgg ttacgttccg ctcaagaaga
     1441 cgggggccaa ctttgccgcc tgttgcccgt tccacagcga gaagtctccc tcctttacgg
     1501 tcagtccaag caagcagttc taccactgct ttggctgcgg cgcgcacggc aacgccatcg
     1561 gctttctcat ggagtattcg ggtctcggct ttgtcgacgc gatcaaggat ctggctggcc
     1621 gtgccggcat gcaggttccc gacgatcacg gacctgccgg gcagcacgcc gagaagtcgc
     1681 aggcactcgg cgagatcatg gcgcgcgctg cccgcttcta ctacgaacag ctcaagggct
     1741 cggcaagggc ggtcagctac ctcaaggagc gcggagtgag cggcgagatc gcgcgccgct
     1801 ttggcctcgg ttatgccccc gagggatggc agaacctggc tgccgctttt gacgactaca
     1861 gcagttccga actgcagctt gcgggactgg tgatcaacaa tgagcgcgga cgcctctacg
     1921 accgctttcg cgagcgggtg atgttcccga tcgtcagcca gaagggcgag gtgatcgcct
     1981 ttggcggccg cgtgcttggc cagggcgaac cgaagtatct gaactcgccc gagaccccgt
     2041 tgttcgaaaa ggggcgcgaa gtgtttggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnncc tgaccgagca gaaaagcatc gctttcgttt tcctccccga gggtgaggac
     2221 cccgacagtc tggtgcgcaa gcgcggcggc gaggccctgc agcggctgat tgcccaggcc
     2281 accccgttgt cggacttcct gttgcgcgaa ctggctggcc attgcgacct caatagtgcc
     2341 gaaggtcggg cccggctggt ggccgacgcc aagccgctgc tggggcgcct gcagtcgtcg
     2401 ctgctgaggt tgcaactggt caagcggctg gccgagagta gcggtttttc gcaggccgag
     2461 gtggagcgcc tgtgcgatct gcaaccgctg gcccgtccgg cgccggcacg ggcgccgcgg
     2521 caagcgcctt cgctgctgcg gcctttgctg cgcctcttgc tgcagaagcc gcaattggct
     2581 ggcggcttgc cgcttgatct gcttcccggc aactccatgg aagcgcgagc ggtgcgcctg
     2641 ttatgcgaaa cgattggcga tggcggggtt cggatgctgg cctatccggc gctgatcgaa
     2701 aagctgcggg gcagcgagta cgagggcatc ctgcgtgccg cggccgctga actaatgcaa
     2761 cagccttttg ccgaagaggg catcgacgcc gagttcgagg gtgcggtcag ccggttgctg
     2821 gaaggtgagc acaagcgggt ttttgccctg ctgcaggaaa aggtacagca acttggggtg
     2881 agcggacttt caagcgaaga aaaacagcag tatctgacag ccctgggtgc gcgcgggcga
     2941 ccagggtccg tcggctaagg tgtgatatca tcaagagttt ttccagtata acgctatccg
     3001 ggaaatggtc atggtcaggg aaaaggcggt aactacgaag gcggcgaaag ccaaggcagc
     3061 ggcggatctg ttggcccagg tcgggacgca accggcaccg gtcgacgacg agacgcgcaa
     3121 gatgcgtctg aagacgctga tcaagctcgg caaggagcgg ggctttctga cctacgccga
     3181 gatcaacgac catctgcctg acgatgtcgt cgatgccgag cagatcgaga gcatcatcag
     3241 caccttcaac gacatgggca tccaggtgta cgacgaggca ccggacgccg aaacgctgct
     3301 gatgtcggac gccgcgcctg ccgccggggc tgacgacgcc gatgtggaag agcaggccga
     3361 gcaggcccta tccaccgttg actccgagtt cggccgcacg accgacccgg tgcgcatgta
     3421 catgcgcgaa atggggtcgg tcgagctgtt gacccgcgag ggcgaaatcg agatcgccaa
     3481 gcggatcgag gacggcctca agcacatgat ccaggcgatt tcggcctgcc cgacgacgat
     3541 cgccgaaatc cttagttgcg ccgacaagat cgcccgcgat gagatgcgca ttgacgagtt
     3601 gatcgatggg ctgatcgacc ctgaaaccga cggatccctg gaagatctga cggctgaggt
     3661 ggccgaggag gaaagcgacg acgaggacga ggaggaagag gacagcgcag aagcagtgga
     3721 gggcgccgcg gtggcggcct cgttgctgaa gctcaagacc gaagggctcg agcgccttga
     3781 actgatccgc tcgcactata ccaaggcgca tggtgtcctg ccgcgcaggg gttcgcagga
     3841 caaggcctat ctgcaattgc aacagcagat ctccgaagag atgatgggga ttcgctttac
     3901 ctcgaagacc atcgagcgcc tgtgcgattc ggtacgcgcc atggtcgagg aggcacgcgc
     3961 ctgcgagcgc aagatccagc gcatctgcgt cgatacggtg cgcatgccgc gcccgcattt
     4021 catcaaggtt ttccccggca acgaactcaa catcgactgg gtggatgcgg agatcgctgc
     4081 tgccggcaag acctacgccg cgatcctcac ccgcaatgcg ccgaatatca aggaagagca
     4141 gcggaaactg cttgccctgc aggatcgcat cggtatcccg ttgcgggaac tgaaggacat
     4201 caacaagcag atgtctaccg gtgaggccaa ggctcggcgt gccaagcgcg agatgaccga
     4261 agccaacctg cggctggtga tctcgattgc caagaaatac accaatcgtg gtctgcagtt
     4321 cctcgatctg atccaggagg gcaacatcgg tctgatgaag gctgttgaca agttcgaata
     4381 ccggcgcggt tacaagtttt cgacctatgc cacctggtgg atccgccagg cgatcacccg
     4441 ctcgatcgcc gatcaggcgc gcacgatccg tatcccggtg cacatgatcg agacgatcaa
     4501 caagatgaac cgcatctccc ggcagatcct gcaggaaact ggcctcgaag ccgatcccgc
     4561 gacgctggcc aagaagatgg agatgcccga agagaaaatc cgcaagatcc tgaagatcag
     4621 caaggagccg atctcgatgg aaacgccgat cggcgacgac gacgattctc atctcgggga
     4681 ctttattgaa gatcaggcta ccctggcacc gaccgaggcg gcgctttact cgagcctgcg
     4741 tttcattacc aaggatgtgc tggacaccct gacgccgcgc gaagccaagg tgttgcggat
     4801 gcgcttcggc atagaaatga ataccgacca cacgcttgag gaagtcggca agcagttcga
     4861 cgtgacccgc gaacgcatcc gtcagatcga agccaaggcg ctccgcaagc ttcg
//
LOCUS       DAOSIT010000119         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15335_length_4913_cov_8.717991, whole genome shotgun sequence.
ACCESSION   DAOSIT010000119 DAOSIT010000000
VERSION     DAOSIT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15335_length_4913_cov_8.717991"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLL14_04190"
     CDS             complement(<1..484)
                     /locus_tag="PLL14_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOG03154.1"
                     /translation="MLASATLRFAQLAVLLLIASAASAQTTYRWIDPSTGGTVVSDRP
                     PPPGARQVVKSAIVGADGPQLPYAVRQASEKFPVILYTSTGCVTCKEARALLEGRGVP
                     FSEKVLQNEEEVAELGRQLGGEALLPSVSVGRQSARGFSPSVWNALLDAATYPSSAPY
                     R"
     gene            complement(496..2595)
                     /locus_tag="PLL14_04195"
     CDS             complement(496..2595)
                     /locus_tag="PLL14_04195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOG03155.1"
                     /translation="MTPNALPDNPLLDFSGLPRFDTLMPGDVQPAITTLLAQGRALIE
                     RLTADDVAATWENFAAPLTDAFEQLSRAWGMVTHVHSVNDVPEWRDAYNSMLPEVSRF
                     YAEIGQNLKLFAGYKAIAEGSEYAQLSTAQRRVLDNEIRDFRLSGAELPAENKPRFQA
                     IAEELAQLSAKFSENLLDATNAFSEMISDESELAGLPEDAREAAREAAERDGRTGWKF
                     TLHMPSYLPVMQYADSRRLRAAMYRAYATRASEFGPEELDNSPLIRRILELRREQAQL
                     LGYDNYAEVSLVPKMAESVPQVLDFLQDLACKARPFAEKDIADLRAFAFAELNLATME
                     AWDVAYVSEKLLQARYAFSEQEVKQYFTEERVLTGLFKVIERLFNVRIKPDAAPTWHA
                     DVRFFRIESTDGELIGQFYLDLYARETKRSGAWMDEAIGRRRVQQTAATTTAPGGIQK
                     PVAYLNCNFSRPIRDTDGKTRPATFTHDEVTTLFHETGHGLHHLLTHVDELGVAGIHG
                     VEWDAVELPSQFMENYCWEWSVLQEMTAHIDTGEPLPRALFDKMLAARNFLSGMQTLR
                     QVEFSLFDLRLHSDFDPAGERSVLDLLAEVRRQVAVLVPPAWHRFPNSFAHIFAGGYA
                     AGYYSYKWAEVLSADCYGAFEEAGDPFDSPTGQRFQSEILALGGSRTAIDNFRAFRGR
                     DPQVDALLRHSGMITND"
     gene            complement(2595..3401)
                     /locus_tag="PLL14_04200"
     CDS             complement(2595..3401)
                     /locus_tag="PLL14_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOG03156.1"
                     /translation="MRLEPTPPARGKTAPKVHSVSATRPFVWLVAGWRDLRANPIASL
                     AFGLLFAIAGDLITIFAWRNGQVFIVATSGFFLVAPLLAGGLYEISRRLETGQASTFF
                     VSLAGGRRNAIELSKLGLLLAAIGLAWERISTLLFAFLAPDITPDLLALLAEIHLSTD
                     HRDLLLIWILIGGTLALIVFAITVISVPLLLDRQTDFRTAMLTSLRAVDANLLVMTFW
                     AFLVVALTALGFVTLFFGLIVLMPLIGHASWHAYRDLVEFPADLLPPRIS"
     gene            complement(3585..4523)
                     /locus_tag="PLL14_04205"
     CDS             complement(3585..4523)
                     /locus_tag="PLL14_04205"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOG03157.1"
                     /translation="MTEALFESTITSLPLLGRGKVRDIYAIGADKLLIVTSDRISAFD
                     VVLPDPVPGKGRLLTRLASFWFARLAEIIPNQLTGIDPETVVATDERQQVRGRALVVK
                     RLRPLPIEAVVRGYLIGSGWQDYQATGSVCGILLPKGLRLASRLPQPVFTPATKAAIG
                     NHDENISFERAQAECATALADLLAATGKSAEAVAGQARDAAIALYATAAKHAAARGII
                     IADSKFEFGLDESGTLHLIDEVLTPDSSRFWPADAYCEGSNPPSFDKQYVRDYLETLD
                     WNKRAPGPRLPAPVLAGTYARYAEACERLTGERVPA"
     gene            complement(4573..4839)
                     /gene="minE"
                     /locus_tag="PLL14_04210"
     CDS             complement(4573..4839)
                     /gene="minE"
                     /locus_tag="PLL14_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOG03158.1"
                     /translation="MSLFSLIFGNKPKTAAIAKERLQLIIAHERSGSASTPDFLPALQ
                     RELMAVISKYVSVNPEDIKVSLEKQGNFEVLEVNIVMPEAGSKP"
BASE COUNT          933 a         1438 c         1614 g          928 t
ORIGIN      
        1 cccggtaggg ggcgctgctg ggataagtgg cggcgtcgag caatgcgttc cagacgctcg
       61 gtgaaaagcc cctggcgctt tgccgtccga cgctcacgct gggcaatagc gcttccccac
      121 cgagttgccg accgagttcg gcgacctctt cctcgttctg aagcaccttc tcggagaacg
      181 gaacgccacg cccctcgagc agggcgcgag cttccttgca ggtgacgcag ccggtgctgg
      241 tgtacaggat caccggaaac ttctcgctgg cctggcggac agcgtaaggg agctgcggcc
      301 cgtcagcacc cactatcgca gacttgacga cctgtctggc ccctggcggc ggtggccggt
      361 ctgaaaccac cgtgccgcca gtgctcgggt cgatccagcg gtaggtggtt tgtgcgcttg
      421 cggcggaggc gatcagcagc agaacggcga gttgggcgaa gcgcaaggta gctgaagcga
      481 gcatgcggcg aaacctcaat cgttagtgat catgccactg tgccgaagca gcgcgtcaac
      541 ctgtgggtcg cggccgcgga aagcccggaa gttgtcgatc gccgtccggc tgccgccgag
      601 agccaggatt tcactctgga agcgttgccc ggtaggtgag tcgaaagggt cgccagcctc
      661 ttcaaaagca ccgtagcagt ccgcagaaag cacctcggcc catttgtagc tatagtaacc
      721 ggcagcgtag ccgccggcaa agatatgcgc gaagctgttc gggaagcggt gccaggccgg
      781 aggtacgagc accgcgactt gccgtcgcac ttccgccaga agatcgagta cgctgcgctc
      841 ccccgccggg tcgaagtcgc tgtgcagccg caggtcgaac agcgaaaact cgacctgccg
      901 gagggtctgc atgccgctca ggaagttgcg cgcggccagc atcttgtcga acagcgcgcg
      961 cggaagcggc tccccggtgt cgatgtgcgc agtcatttcc tgcagcacgc tccactccca
     1021 gcaatagttt tccatgaact gcgagggcag ctcgacagcg tcccattcga cgccatgaat
     1081 gccggcaacg ccaagctcat cgacatgcgt cagcaggtgg tgcagaccgt gcccggtctc
     1141 gtggaacagt gtggtcacct cgtcatgcgt gaaagtcgcc ggccgtgttt ttccatccgt
     1201 atcgcgtatc ggtcgagaaa agttgcagtt gaggtaggcg accggcttct ggatgccgcc
     1261 aggagcggtt gtagtcgccg cggtttgctg cacgcggcgg cgaccgatgg cttcgtccat
     1321 ccaggcgcca ctgcgcttcg tctcgcgggc gtagaggtcg agatagaatt ggccgatcaa
     1381 ctcgccgtcg gtgctctcga tgcgaaaaaa ccgtacgtcc gcatgccatg ttggcgcagc
     1441 gtcgggcttg atacggacgt tgaacaacct ttcaatgacc ttgaacagac ccgtcagcac
     1501 gcgttcctcg gtgaaatact gcttgacctc ctgttccgag aaagcatagc gcgcctgcag
     1561 aagcttctcc gacacatagg cgacatccca ggcttccatt gttgcgaggt tcagttcggc
     1621 gaatgcgaag gcacgcagat cggcgatatc cttctcggca aagggcctgg ccttgcatgc
     1681 cagatcctgc aggaaatcga ggacctgcgg caccgattcg gccatcttcg gtaccagcga
     1741 aacttctgca tagttgtcgt agccgagaag ctgcgcctgt tcgcggcgca gttccaggat
     1801 gcggcgaatc agcgggctgt tgtcgagttc ttcggggccg aattcggagg cgcgtgtggc
     1861 gtaggcgcgg tacatcgccg cgcgcaggcg gcggctgtcg gcgtattgca tcaccggcag
     1921 gtaggatggc atgtgcagag tgaacttcca gcctgttctg ccgtctcgtt ccgccgcttc
     1981 acgcgccgct tcacgcgcgt cttcaggcag accagcgagt tcgctctcgt cgctgatcat
     2041 ttcggaaaag gcgttggtcg cgtcgagcag gttctccgag aactttgccg agagttgtgc
     2101 aagttcctcg gcaatggcct gaaagcgcgg cttgttctcg gcgggcaact cggcaccact
     2161 cagccggaag tcgcgaatct cgttgtcgag cacccggcgc tgggcagtgg acaactgcgc
     2221 gtattcactg ccttcggcga tcgccttgta gccggcgaac agcttcaggt tctggccgat
     2281 ttcggcatag aagcgggaga cttcggggag catgctgttg taggcgtcgc gccactcagg
     2341 cacatcattg accgagtgca catgcgtgac catcccccag gcgcgtgaca gttgctcaaa
     2401 ggcgtcggtg agtggtgcag cgaaattttc ccaggtcgcg gccacatcgt cggccgtcag
     2461 gcgctcgatg agggcgcgac cttgcgcgag cagtgtggtg atcgccggtt gcacatcgcc
     2521 cggcatcagc gtgtcgaagc ggggcagtcc ggagaagtcg aggagcgggt tgtctggcag
     2581 ggcattgggt gtcatcagga gatccgtggt ggcagaaggt cggctgggaa ttctacaagg
     2641 tctcgataag catgccagga agcgtggccg atcagtggca tcagaacgat cagtccgaag
     2701 aacagggtga cgaaacccag cgcggtcagc gcgacgacca ggaacgccca gaaggtcatc
     2761 accagcaggt tggcgtctac tgcgcgcagg ctggtcagca tggctgtccg gaagtcggtc
     2821 tggcggtcaa gcaacagcgg tacggagatc accgtgatcg cgaagacgat caacgccagg
     2881 gtgccaccga tcagaatcca gatcaacaac aggtcgcggt ggtctgtgga aaggtggatc
     2941 tcggccagca gggcgagcag atcgggggtg atatcgggtg ccaggaaggc gaacagcaag
     3001 gtcgagatgc gttcccaggc gagtccgatg gctgccagca gcaggccaag cttgctcagt
     3061 tcgatggcgt tgcgtcggcc gccggccaac gacacgaaga aggtcgacgc ctgaccggtt
     3121 tcaagtcgcc ggctgatttc gtagagtccg ccggcgagca gtggggcgac caggaaaaag
     3181 ccggaagtgg cgacgatgaa cacctgtcca ttgcgccagg caaagatcgt gatcaggtcg
     3241 ccggcgatcg cgaacaagag gccaaacgcg aggctggcaa tcgggttggc gcgcagatcc
     3301 cgccagcctg ccaccagcca cacgaatggg cgggtggcgg atacgctgtg tactttgggc
     3361 gctgtcttgc cgcgggcagg gggagttggc tccagacgca tgcgttctcc ttgctgaggg
     3421 ggtggatgtg cagttctcgt cagcgactcg cgctttcatc ggcagacata caggaaaggg
     3481 gacgaaagcc gcaaaaggcg gtcttcttgg tcaccatcga gcgtaccgga acctgtccac
     3541 gggcgtgcaa ggtggatctc tggctgtcgg tgctgccggc tcggtcaagc aggtacccgt
     3601 tcgccggtca ggcgttcgca ggcctcggcg tacctggcgt aagtgccggc gagtaccggc
     3661 gcgggcaggc gcgggccagg cgctcgcttg ttccagtcca gcgtttcaag atagtcgcga
     3721 acatactgct tgtcgaacga cggcggattg ctgccttcac agtaagcgtc ggcgggccag
     3781 aaacgggacg agtccggggt cagaacttcg tcgatcaggt gcagcgtgcc ggactcatca
     3841 aggccgaatt cgaacttgct gtcggcaatg atgatgccgc gcgctgcagc gtgctttgcc
     3901 gccgtagcgt acagcgcgat ggctgcgtcg cgggcctgtc cagcgacggc ttcggcactc
     3961 ttgccggtag ccgcgagcag gtcggcgagt gccgtggcgc attcggcctg cgcacgttcg
     4021 aaggaaatgt tttcgtcgtg attgccgatt gctgccttgg tcgccggggt gaagaccggc
     4081 tgcggcaacc ggctcgcgag ccgtagccct ttgggcagaa ggatgccgca aacactgccc
     4141 gtggcctggt agtcctgcca tcccgaaccg atcagatagc cacgtaccac cgcttcgatc
     4201 ggcagtggtc gcaaccgttt gacgaccaac gcccgacctc gcacctgttg tcgttcgtcg
     4261 gttgccacca cggtttccgg atcgatcccg gtcagttggt tcgggatgat ctcggcgagc
     4321 cttgcaaacc agaagctggc caggcgcgtc agcagcctgc ccttgccggg aacggggtcg
     4381 ggcagaacga catcgaaggc cgaaatacga tcgctggtga cgatcagcag cttgtcggcg
     4441 ccaatcgcgt agatgtcccg caccttgccg cggccgagca aaggcaagct ggtgatcgtc
     4501 gattcaaaaa gggcttcggt cacggtgttg atccggaagg cgaatgaatg ggggatcggg
     4561 aaacggctat attcagggtt tgctgcccgc ttccggcatg acgatgttga cctccagcac
     4621 ctcgaagttc ccctgcttct cgagcgacac cttgatgtct tcggggttca ccgacacata
     4681 tttcgagatc acggccatca actcccgctg cagggccggc aggaaatccg gcgtactggc
     4741 gctgccactg cgttcgtggg caatgatcag ttgcagtcgc tccttggcaa tcgcggccgt
     4801 tttgggcttg ttgccgaaga tcagggagaa cagggacatc ctcatttacc cccgaacagc
     4861 cgcttgaaga gaccgggttt ttcgtagtcc acgaaacgca agggcacgct ttc
//
LOCUS       DAOSIT010000120         4912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15341_length_4912_cov_9.606753, whole genome shotgun sequence.
ACCESSION   DAOSIT010000120 DAOSIT010000000
VERSION     DAOSIT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_4912_cov_9.606753"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2594)
                     /locus_tag="PLL14_04215"
     CDS             complement(<1..2594)
                     /locus_tag="PLL14_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOG03159.1"
                     /translation="MPTVTESTHNLRRNLREIAEHQNLSARVGEYLDSYFVDVELNAA
                     SDASPEELLGAATQHFRLGEVRPARAAVSALYTPDFDRHGWHSPHTVIDVVTDDMPFL
                     VDSITMVVYRRGLVIHRLLHPLISAERDAEGKLLRTLPRGAAGSQPESWIHLEIDRIG
                     DTDLIADLHREIVEVLADVRAAVEDGATMQQRMHEADVEMAAATAEACDEVAAYLRWI
                     AVNNFVFLGYAEYRAATGSRSLTRVAESGLGILRHPDHPRFGTCLAGIPGVVAELAKD
                     PLPAILVKADARSTVHRSAYLDFIGVKCYDASGNIVGVRAFVGLYTAHVYHVAATDVP
                     LLRRKIAAVRQEIGFLPRSHRDKTLLNVLETYPRDELIEIAHDDLIRIARGIVSLYER
                     EQVRVFLRDDAWGRYVSVIVYMPRDRFDTVLRERISALLDETLAAERVDFFVMLGESR
                     LARLHFIARTPVGTRYAYDAGAIERQVARIVRGWSDELKQNLVGHYGEERGNSLLRRY
                     ANELPLSYQERVTPASAVSDLERLEAAEQSGRVEVKLSAAQGDDGAHQHLKLFRRGRP
                     RPLSAILPVLENMGLTVLSEQPFSLPRSDLHIADFAVQLPQAAALDDDSTRRAFVELL
                     ESLLSDEAENDGFNRLVLLAGLDGRQIGILRAYSRYLRQAGLPFSQAYVQRCLARHFQ
                     ITRALVEFFEALFSPSADDARARALSDEVDTALLQVSNPDDDRILAALQTAIEATLRT
                     NAYQTGRDGKAKDYLSFKFSSQHIPFLPAPAPLYEIFVYNERVEGVHLRGARVARGGL
                     RWSDRMEDFRTEVLGLVKAQMVKNAVIVPLGSKGGFVCKRLPPAGEREAFQAEGIACY
                     SIFIRGLL"
     gene            complement(2724..3452)
                     /locus_tag="PLL14_04220"
     CDS             complement(2724..3452)
                     /locus_tag="PLL14_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOG03160.1"
                     /translation="MISMSKVSKHYGSFQVLADCTTQVHKGEVIVVCGPSGSGKSTLI
                     KCVNGLEPFQSGEIIVNGVSVGDPKTNLSKLRSTVGMVFQNFELFPHMSIIDNLGIAQ
                     VKVLGRTQDQAREKGFKLLDRVGLRAQAEKYPGQLSGGQQQRVAIARALAMDPICMLF
                     DEPTSALDPEMVNEVLDVMTELAQEGMTMMCVTHEMGFAKRVASRVIFMDQGRIVEDD
                     SKDAFFDHARSERAQQFLAKILHH"
     gene            complement(3529..4209)
                     /gene="gltK"
                     /locus_tag="PLL14_04225"
     CDS             complement(3529..4209)
                     /gene="gltK"
                     /locus_tag="PLL14_04225"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HOG03161.1"
                     /translation="MHEFDWSSIPNALPFLWDGMKISLEITVTAVIFGIVWGTLLALM
                     RLSSVKPLSWFAAGYVNLFRAIPLVMVLLWFFLIVPHLLESIFGFPRGSDMRLSSAIA
                     GFALFEAAYYSEIIRAGIQSVPKGQMGAASALGMTYGQAMRLVVLPQAFRNMVPLLLT
                     QGIILFQDTSLVYVSALADFFGAAYKVGDRDGRLVELLLFAGAVYFVICFTASRIVRH
                     YQNKLKIA"
     gene            complement(4211..>4912)
                     /locus_tag="PLL14_04230"
     CDS             complement(4211..>4912)
                     /locus_tag="PLL14_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG03162.1"
                     /translation="PTATGDDTYLGWMFYGFKMTIALSLSAWLIALAVGAVVGVLRTV
                     PNKWLSGIATAYVEIFRNIPLLVQLFLWYFVMPELLPESVGNAFKQTNPIMQQFLASM
                     VCLALFTSARVAEQVRSGINSLPPGQKNAGLAMGFTLPQTYRYVMLPMAVRLIVPPLT
                     SEFLNIFKNSAVCSTIGLLELAAEGRQLVDYTAQPYESFIAVTLAYLLINVVVMFGMR
                     WVEEHVRVPGYLGGK"
BASE COUNT         1057 a         1508 c         1462 g          885 t
ORIGIN      
        1 tcgagcaggc cgcgaatgaa gatcgaataa caggcaatac cctcggcctg aaaggcttct
       61 cgttcgcccg caggcggcag acgcttgcag acgaacccgc ctttcgaacc cagaggaacg
      121 atcaccgcgt tcttcaccat ctgcgccttg accagaccga gcacctcggt gcggaagtct
      181 tccatgcgat cggaccagcg caggccgcca cgcgccaccc gcgcaccacg cagatggacg
      241 ccttcgacgc gctcgttgta cacgaatatc tcgtatagtg gcgccggtgc gggcaggaag
      301 ggaatgtgct gcgaggagaa cttgaacgac aggtagtctt tggcctttcc atcgcggcca
      361 gtctgataag cgttggtccg caaggtggct tcaatcgccg tttgcagcgc agcaagaatg
      421 cgatcatcat cgggattgct gacctgcagc aaggccgtgt ccacttcatc ggagagtgct
      481 ctggcgcgcg catcgtctgc acttggagaa aaaagggctt cgaagaactc gaccagggca
      541 cgcgtgatct gaaaatgcct ggccaggcag cgttgcacat aggcctggct gaatggcagg
      601 ccagcctgac gcaggtagcg gctgtaggcg cgcagaatgc cgatctgtcg gccgtccaga
      661 cctgccagca gaaccaggcg gttgaaaccg tcgttctcgg cctcgtcact gagcaggctt
      721 tccagcagtt cgacaaacgc ccgtcgtgtg ctgtcgtcgt cgagggccgc cgcttgcggc
      781 aactgcacgg cgaagtcggc aatgtgcagg tcgctcctgg gcaggctgaa tggctgctcg
      841 gaaagcaccg tgagtcccat gttttccagc accggcagga tcgccgaaag cggccgcggg
      901 cggccgcggc ggaacagttt caggtgctgg tgggcaccgt cgtccccttg cgcggcgctc
      961 agcttgactt cgactcgacc actctgctct gccgcttcca ggcgttcgag gtcagatacc
     1021 gctgacgctg gcgtgacgcg ctcctgatag gaaagaggca actcgttggc ataacgacgc
     1081 agcaggctgt tgccccgttc ctcgccgtag tgccccacga ggttctgttt cagttcgtca
     1141 ctccagccgc gcacgatgcg cgccacctgc ctttcgatgg caccagcgtc gtaagcgtaa
     1201 cgcgtgccga ccggcgtccg ggcgatgaaa tgcaggcgcg ccaaccgcga ttctccgagc
     1261 atgacaaaga agtccacgcg ctccgcggcc agggtttcgt cgagcaatgc cgagatccgc
     1321 tcgcgcagga cggtatcgaa acggtcgcgc ggcatataga cgatcaccga cacatagcgc
     1381 ccccaggcat cgtcgcgcag gaagacgcgc acctgttcgc gttcgtacaa ggacacgatg
     1441 ccacgcgcaa tgcggatcag atcgtcgtgc gcgatttcga tcagttcatc acgcggataa
     1501 gtttcgagga cattgagcag cgtcttgtca cggtggctgc gtggcagaaa accgatttcc
     1561 tgtcgcacgg cggcaatctt gcgccgcaac aagggtacgt cggttgccgc cacatggtag
     1621 acgtgggcgg tgtacaggcc gacgaaggcc cgtacaccga caatgttgcc gctcgcgtca
     1681 tagcacttga cgccgatgaa gtcgaggtag gccgagcgat gcacggtcga gcgggcatcg
     1741 gccttgacca ggatggcagg caagggatcc tttgcgagtt ccgcgacaac acctggaatc
     1801 cctgccaggc aagtgccgaa gcggggatga tcagggtggc gaagaattcc cagtccgctc
     1861 tcggcaacac gcgtgaggct gcgactgcca gtcgccgcac ggtattcggc gtaacccaga
     1921 aagacaaaat tgttgacggc gatccagcgt agataagccg cgacttcgtc acaagcctcg
     1981 gccgtcgctg cggccatctc gacatccgct tcatgcatac gttgctgcat ggtcgcgcca
     2041 tcttccaccg cagcacgcac atcggcgagt acctccacaa tttcgcggtg caggtcggcg
     2101 atcaggtcgg tatcgccgat gcggtcaatc tccaggtgaa tccaggattc aggctggcta
     2161 ccggcggcgc cacgcgggag ggtgcgcagc aactttcctt cggcgtcgcg ttcggcactg
     2221 atgaggggat gtagcaagcg atggatgacc aagccgcgac ggtacaccac catggtgatc
     2281 gaatcgacca gaaagggcat gtcatcggtg acgacatcga tcacggtgtg cggcgaatgc
     2341 cagccgtgac ggtcgaaatc tggggtgtat agcgcgctga ccgctgcgcg tgccggacga
     2401 acttcgccca gacgaaaatg ctgcgtcgcc gcgcccagca gttcttccgg actggcgtcg
     2461 ctggcagcat tcagttcgac atcgacgaaa taactgtcaa ggtactcgcc gacgcgtgcc
     2521 gagaggttct gatgctcggc gatctcgcgc aggttgcggc gcaggttgtg ggtcgattct
     2581 gtcaccgttg gcatgccgct ctcctctcgc gatgtcgatc gagtcgctac gttccacact
     2641 ctggtcgccg acgcctgcgc agccgaaacc tctggactac acgcgacgac actccggacg
     2701 ggcagttggt cagcggcaca cgctcagtga tgcagaatct tggccaggaa ctgttgcgcc
     2761 cgctccgaac gggcgtggtc gaagaaggca tccttgctgt cgtcctcgac gatccgcccc
     2821 tgatccatga agatcacccg actggcaacg cgtttcgcaa atcccatctc gtgcgtgacg
     2881 cacatcatgg tcatcccttc ctgcgccagt tcggtcatca cgtcgagcac ttcgttgacc
     2941 atctccgggt cgagcgccga cgtaggctcg tcaaacagca tgcagattgg gtccatggcc
     3001 agagccctgg caatcgccac gcgctgctgc tggccgccgg agagctgacc gggatacttt
     3061 tccgcctgcg cgcgcagacc gacccgatca agcaacttga aacccttctc gcgcgcctga
     3121 tcctgtgtgc gcccaagcac cttgacctgg gcaatcccca ggttatctat gatgctcatg
     3181 tgcgggaaga gttcgaagtt ctgaaacacc atgcctaccg tcgagcgcag cttggaaaga
     3241 ttggttttcg gatcgcctac ggacacgcca ttgacgataa tttcaccgct ttgaaagggt
     3301 tcgaggccat tgacgcactt gatcagcgtt gacttgcccg aaccggacgg accacagacg
     3361 acaatcactt cgcccttgtg gacctgcgtc gtgcagtcgg caagaacctg gaaggaaccg
     3421 taatgcttgc tgaccttgct catcgaaatc atcggaaatc tcctcggtcg ttactgcaga
     3481 acggcagcct tccgttctgg gggtttggta ttggcatcaa gcagcctctc aagcgatctt
     3541 gagcttgttc tgatagtggc gaacgattcg cgacgcggta aagcagatca cgaaatagac
     3601 ggcaccggca aagagcagca gttcgaccaa ccgcccgtcg cggtcgccaa ccttgtaggc
     3661 cgcaccaaag aagtcggcga gggccgaaac gtagaccagc gaagtatcct ggaacaggat
     3721 gattccctgc gtcagcaaca gaggcaccat gtttcggaaa gcctgcggca gcaccaccag
     3781 tcgcatcgcc tggccatagg tcatgcccag ggcactggcg gcgcccatct gacctttcgg
     3841 aaccgactgg ataccggcgc ggatgatctc cgaataatag gccgcctcga acagcgcgaa
     3901 accggcgatc gccgacgaca ggcgcatgtc cgaaccgcga ggaaagccga agatgctttc
     3961 cagcagatga ggcacgatca ggaaaaacca caacagcacc atgaccagcg gtatcgcccg
     4021 gaacaggttg acgtaacccg cggcaaacca ggacaggggc ttgaccgagg acagtcgcat
     4081 cagggccagc agggtacccc agacaattcc gaagatcacg gcagtcaccg tgatctccag
     4141 agaaatcttc attccatccc acagaaaggg caaggcattg ggaatcgaag accagtcgaa
     4201 ttcgtgcatt tcacttgcct ccaagatagc cgggaacgcg cacgtgctct tcaacccagc
     4261 gcatgccgaa catcaccacc acgttgatca acaggtaggc aagcgtcacg gcgatgaacg
     4321 actcgtacgg ctgcgccgtg taatccacca gttgccgacc ctccgcggcc agttcaagca
     4381 ggccgatcgt cgaacacacg gccgagttct tgaagatgtt gaggaactcc gaagtcaacg
     4441 gtgggacgat cagccgcacc gccatcggca acatcacgta gcggtaggtc tgcggcagcg
     4501 tgaaacccat cgccagaccg gcgttcttct ggccgggggg caaggaattg atccccgaac
     4561 ggacctgttc ggcaacacgg gcgctggtga acaaagccag gcagaccatc gaagcgagaa
     4621 actgctgcat gatcggattg gtctgcttga aagcgttgcc gacgctctcg ggcaacagct
     4681 ccggcatcac gaaataccag aggaacagtt gcaccagcag cggaatgttg cgaaagattt
     4741 cgacgtaggc cgtggcgatt cccgacaacc acttgttcgg gaccgtccgc aggacgccga
     4801 cgacggcccc cactgccagc gcaatcagcc acgccgacag cgacagggcg atggtcatct
     4861 tgaagccgta gaacatccag ccgagatacg tatcgtcgcc cgtcgccgtc gg
//
LOCUS       DAOSIT010000121         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15393_length_4898_cov_10.489366, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000121 DAOSIT010000000
VERSION     DAOSIT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15393_length_4898_cov_10.489366"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLL14_04235"
     CDS             <1..945
                     /locus_tag="PLL14_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOG03163.1"
                     /translation="CITVPQDGAGYARSSALPAPPDPADNPTTPAKAALGKQLFFDAR
                     LSGDGSMACQGCHFRHLGWTDGLALSRKVGGGMNTRHTPSVYNTGYYTAWYWDGRATT
                     LEGQVTAAWKAQIGADPVKAAAVIAAVPGYQVEFQKVFGAPPDADNIAKALAAFLRTL
                     NSGEAPWDRHAAGDKAAVSTAALAGYELFVGKGGCVACHKPPLFSDSQFYNIGLEAGK
                     PKPDVGRNAVTKNPADMSAFKTPTLRSVGISGPYFHDGSVTSLEDAVRYMASGGRADA
                     NKSALLIDRKLSDQEIGQIVAFLNTLTSDERFEPPKLP"
     gene            1043..1735
                     /locus_tag="PLL14_04240"
     CDS             1043..1735
                     /locus_tag="PLL14_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HOG03164.1"
                     /translation="MHALHRLSTADWLALFVFIACWAGYAWFSEHSRWASAGLIRSGH
                     GFRLEWAYRMLEREVRVTDSTLIGNLVTSVTFYANTTIYIIAGLVAALGAADKLLTFT
                     VDMAFGGAGNRELLEIKLMLVLASFVYAYFKFTWSLRQFNVLSILVGAAPLGKRDDPA
                     IASYARRVAAANNLAGDDFNRGIRAYYFGLAASGWLLSPLSLAVLSVLILVVLYRRDY
                     RSAALDVLRETH"
     gene            1740..2627
                     /gene="rarD"
                     /locus_tag="PLL14_04245"
     CDS             1740..2627
                     /gene="rarD"
                     /locus_tag="PLL14_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOG03165.1"
                     /translation="MTGNRHLAGIVFGLLAYAMWGFFPLFFRQLAHVSPIDVLSNRAV
                     WACVFVGVLLSLRRQWHKVAAVFRSARQVLLLLIASLLIGSNWLVFLWAVANQQVVAS
                     SLGYFLTPLVNVLLGLLVLKERLNRLEWLSVALALAAMANEIVAIGHLPWVSLVLAGT
                     FGTYGLVRKQLPVDAVSGLWLETLAMLPVCALYAWWQAPSGHAVFTGHDPATTVLLVG
                     SGALTALPLLAFAAATQRLDLASVGMLMYINPTLQFVTAIWVFGEPMRRAQLLSFGLI
                     WLGLLVFSYSAWGKYRRPA"
     gene            3013..4695
                     /gene="ilvB"
                     /locus_tag="PLL14_04250"
     CDS             3013..4695
                     /gene="ilvB"
                     /locus_tag="PLL14_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HOG03166.1"
                     /translation="MPKQPREILTGAQIVVRLLERQGVRTLAGIPGGAILPIYDALSK
                     SELIHHVLARHEQGGGFMAQGMARASGLPAVCMASSGPGATNLLTAIADAKLDSIPLV
                     AITGQVPRAMIGTDAFQEVDTYGLSIPITKHNFLVNAAVDLLEIIPRAFRIAASGRPG
                     PVLVDIPKDVQNQLVEVAEWPAPGGAEPVPPPAAEAVERAAAMINAAARPILYLGGGV
                     VHSAASGLAVAFAEKCRLPTVMTLMALGAMPVDHPLSLGMLGMHGARCTNLALDECDL
                     LIAVGARFDDRATGKVAGFCPQAQIIHIDIDPSELDKIKTAHVGIAGDVSSVLEMLLP
                     ALAVVERDDWLARVASLKAAQPLRTPGIDDPRTPYGLIRAVADCLDDAATITTDVGQH
                     QMWVAQAYPLRRPRQWLTSGGLGTMGFGMPAAIGAALAEPERTVVCFTGDGSILMNVQ
                     ELVTAAEENVNVKIVLMNNSSLGLVFQQQTMFYGERIYASKFKGMPDFVRVAEGFGVA
                     AIDLDKEVEPLAALARALSTRGPMLIHASIAMHEQVLPMVPPGAANKEMIGG"
     gene            4699..>4898
                     /locus_tag="PLL14_04255"
     CDS             4699..>4898
                     /locus_tag="PLL14_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOG03167.1"
                     /translation="MNSIARSENQALARTVLELDVNNHPGVMSHVVGLFSRRAYNVEG
                     ILCMPVGSGDTSRIWLLVNEDER"
BASE COUNT          768 a         1587 c         1611 g          932 t
ORIGIN      
        1 tgcatcacgg taccgcagga tggcgccggc tatgcccgct cgtcggcctt gccggcgccg
       61 ccggatccgg ccgataaccc gacgacgccc gccaaggcgg cgctcggcaa gcaactgttt
      121 ttcgatgcgc gtctttccgg cgacggcagc atggcctgcc agggttgcca cttccgtcat
      181 ctcggatgga ccgacgggtt ggcgttgtca cgcaaggtcg gtggcggcat gaacacccgt
      241 cacaccccca gcgtctacaa caccggctac tacacggcct ggtactggga cgggcgcgcg
      301 acgacgctgg aggggcaggt cacggcggcc tggaaggcac agatcggtgc cgacccggtc
      361 aaagctgcgg cggtcatcgc cgccgttcct ggctaccagg tcgagttcca gaaagtcttc
      421 ggcgccccgc cggacgcgga caacattgcc aaggcgctgg ccgcgttcct gcgcaccctg
      481 aacagcggcg aagcgccgtg ggaccgccat gccgccggcg acaaggccgc tgtttcgacc
      541 gctgcgctgg ccgggtacga gttgtttgtc ggcaagggcg gttgtgtcgc ctgccacaag
      601 ccaccgctgt tcagcgacag ccagttttac aacatcggat tggaagccgg caagccgaaa
      661 cccgatgttg gccggaacgc ggtgaccaaa aaccctgcgg acatgtcggc gttcaagaca
      721 ccgacgctgc ggtcggtcgg catttccggg ccgtacttcc acgacggcag cgtcaccagt
      781 ctggaagatg cggtgcgcta catggcctcc ggcggccggg ccgacgccaa caagagcgcg
      841 ctgttgatcg accgcaagct ttccgaccag gagatcggcc agatcgtcgc cttcctgaat
      901 acgctgacca gcgacgagcg tttcgagcct ccgaagctgc cgtaggccga tctcgcacct
      961 ccggcacgtc gcgcggcacg ccacggtgcc ggtggcgccg cgccgacctt gacggctgca
     1021 cgttctgtgg gaagatgagc ggatgcacgc actccaccgt ctctcgaccg ccgactggct
     1081 ggcgctgttc gtcttcattg cctgctgggc gggctatgcc tggttctccg agcatagccg
     1141 ctgggcttcc gcgggactga tccgcagtgg ccacggcttc cggctcgaat gggcctatcg
     1201 catgctcgag cgcgaggtgc gggtgaccga ctcgacgctg atcggcaatc tggtgaccag
     1261 cgtcactttc tacgccaata ccacgatcta catcatcgcc ggtctggtcg ccgcgctcgg
     1321 tgcggcggac aagctgctga ccttcaccgt ggacatggcg ttcggcggtg ccggcaaccg
     1381 cgaactgctg gagatcaagc tgatgctggt gctggccagt ttcgtttacg cctatttcaa
     1441 gttcacctgg tcgctgcgtc agttcaatgt gctctcgatc ctggttggcg cggcaccgct
     1501 cggcaagcga gatgatccgg ccatcgcgag ctatgcccgg cgggtggcgg cggccaacaa
     1561 tctggccggc gacgacttca atcgcggcat ccgcgcctac tatttcggcc tggccgcatc
     1621 cggctggttg ctcagcccgc tgagcctggc cgtgctttcg gtgctgatcc tcgtggtgct
     1681 ctatcggcga gactaccgct cggcggcact cgacgtgttg cgcgagacgc actgaagcgg
     1741 tgaccggcaa ccgccatctg gccgggatcg ttttcggcct gcttgcctat gcgatgtggg
     1801 gattctttcc gctgtttttt cgccagctcg cgcacgtttc gccgatcgat gtgttaagca
     1861 accgcgccgt ctgggcctgc gttttcgtcg gcgtgctgct gagcctgcgg cggcagtggc
     1921 acaaggtggc ggcggtgttc aggagtgcgc ggcaggtgct gctgctgctg atcgcttcct
     1981 tgttgatcgg gtccaactgg ctggtcttcc tgtgggccgt cgccaatcag caggtcgtcg
     2041 cctccagtct cggatacttc ctgactccgt tggtcaacgt cctgctcgga ctgctggtcc
     2101 tcaaggaacg gctgaaccgg ctggaatggc tttccgtcgc tctggctctg gcggcgatgg
     2161 ccaacgagat cgtcgccatc ggtcatctgc cctgggtgtc gctggttctc gccggcacct
     2221 tcggcaccta cggcctggtg cgcaagcaat tgccggtcga tgcggtctcc gggttatggc
     2281 tggaaaccct ggcgatgctg ccggtttgcg cgctgtatgc gtggtggcag gcaccgagcg
     2341 gccatgcggt gttcaccggt cacgatccgg cgaccacggt cctgctggtg gggtcgggcg
     2401 cgctcaccgc actgccgctg ctggcctttg ccgccgccac acagcggctc gatctggcca
     2461 gcgtcggcat gctgatgtac atcaacccga cgctccagtt cgtgaccgcg atctgggtct
     2521 tcggcgaacc gatgcggcgt gcgcaactgc tcagcttcgg gttgatctgg ctgggcctcc
     2581 tggtgttcag ctacagcgcg tggggaaagt accgcaggcc ggcttaggcg cggcgcacgg
     2641 gtctgcggaa cctgaccgcc gtggtctgga atcgccggca tcgacatgct aagatcgccc
     2701 tctttgcagc cccgtctgtg ccctgatgtt cgggtctctc gtagggctgc aggctgactt
     2761 tccccgctcg catctgccaa gttgatctgc aagaccacaa gcgcattcag gccggcagga
     2821 tgaactcctg ccgcgtgcgc gcctccaact ccccgccatg tcgtcgtgcg cagcgcgcat
     2881 ggcgctgttg gcgccagacg aacgaccacc aatttcagaa agcctcgccg gtgcaagccg
     2941 tgcggcgctt gcgcttttcc ggacccaagc cgggcgacgc cgcaggcggc agtcctatcg
     3001 aaggagattg atttgcccaa gcaaccacgc gaaatcctta ccggcgcaca gattgtcgtg
     3061 cgtctgctcg aacgacaggg cgttcgtacg ctggccggca ttcccggcgg cgccatcctg
     3121 ccgatctacg acgccttgtc gaagtcggag ctgatccacc atgtcctggc ccggcacgag
     3181 cagggtggcg gcttcatggc gcaggggatg gcgcgtgcca gcggtttgcc ggcggtgtgc
     3241 atggcttcct cgggtcccgg cgccaccaat ctgctgacgg cgatcgccga cgccaagctg
     3301 gattcgattc cgctggtagc gatcaccggg caggtgccgc gagcgatgat cggtaccgat
     3361 gccttccagg aagtcgacac ctacggcttg agcatcccga ttaccaaaca caacttcctg
     3421 gtcaatgccg ccgtcgatct gctggagatc attccgcgtg ctttccgcat cgcggcttcg
     3481 ggtcgtcccg ggccggtgct ggttgatatt ccgaaggatg tgcagaatca actcgtcgag
     3541 gtcgccgaat ggcccgcgcc gggtggcgcc gagcccgttc cgccaccggc cgcggaagcg
     3601 gtcgaacgtg cggcggcgat gatcaacgcc gcagccaggc cgattctcta ccttggcggg
     3661 ggggtggtgc attccgccgc ctccgggctg gccgtggcgt tcgccgaaaa gtgtcggctg
     3721 ccgacggtca tgaccctgat ggccctcggg gcaatgccgg tcgatcatcc gctgtcgctc
     3781 ggcatgctgg gcatgcacgg ggcgcgctgt accaacctcg cgctcgacga gtgcgacctg
     3841 ctgatcgccg tcggggcccg cttcgatgac cgggcgacgg gcaaggtggc cggtttctgc
     3901 ccgcaggcac aaatcatcca catcgacatc gacccctccg aactcgacaa gatcaagacc
     3961 gcgcatgtgg gcattgccgg cgacgtgagt tcggtgctgg agatgctcct gccggcactg
     4021 gcggtcgtcg agcgggatga ctggctggcg cgcgtggcca gcctgaaagc ggcgcagccc
     4081 ctgcgcacgc cgggcatcga tgacccgcgc accccttacg gcctgatccg cgcggtcgcc
     4141 gattgcctcg acgatgcggc gacgattacc accgacgtcg gtcagcacca gatgtgggtg
     4201 gcgcaggcct accccttgcg ccggccccgg cagtggctga cgtccggcgg tctcgggacg
     4261 atgggcttcg gcatgccggc ggccatcggc gcggcactgg ccgagccgga acgaacggtg
     4321 gtctgtttta ccggcgacgg cagcattctg atgaatgtcc aggaactggt gactgccgcc
     4381 gaggagaacg tcaacgtcaa gatcgtgttg atgaacaact cgtcgctcgg tctggttttc
     4441 cagcagcaga cgatgtttta cggcgagcgc atctacgcct cgaaattcaa gggcatgccc
     4501 gacttcgtgc gcgtcgccga gggtttcgga gtggccgcca tcgacctcga caaggaggtc
     4561 gagccgctgg ccgctttggc cagggcgctg tcgacgcgcg gtccgatgct gattcacgcc
     4621 agcatcgcca tgcacgaaca ggtgttgccg atggtgccgc cgggtgcggc caacaaggaa
     4681 atgatcggag gctgaacgat gaattcgatt gcacgcagtg aaaaccaggc gctggcgcgc
     4741 acggtgctcg aactggacgt caataaccac ccgggagtca tgtcgcacgt cgtcggcctc
     4801 ttttcgcgtc gggcgtacaa cgtcgaaggc atcctgtgca tgccggtggg gagcggcgac
     4861 accagtcgca tctggctgct ggtcaatgag gacgagcg
//
LOCUS       DAOSIT010000122         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15519_length_4862_cov_9.830040, whole genome shotgun sequence.
ACCESSION   DAOSIT010000122 DAOSIT010000000
VERSION     DAOSIT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15519_length_4862_cov_9.830040"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PLL14_04260"
     CDS             <1..164
                     /locus_tag="PLL14_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOG03168.1"
                     /translation="ALAHKMLRTSFFMLKRGDYYRDSATNYEQLSVQRNASRWIKALI
                     RFGFIPAAA"
     gene            complement(166..495)
                     /locus_tag="PLL14_04265"
     CDS             complement(166..495)
                     /locus_tag="PLL14_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03169.1"
                     /translation="MLLGQNAQLIPPGYDLTEAQHDLATYVQRHPRFSRLRQLIRKAK
                     DTEMALGSDILIAALKGYALAKAIGKGAGLDALKETMATRFINVKEPSLQAHGCLTAS
                     GHGDRWS"
     gene            complement(592..822)
                     /locus_tag="PLL14_04270"
     CDS             complement(592..822)
                     /locus_tag="PLL14_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG03170.1"
                     /translation="MQSIKVLGTGCANCRATVAVIEAVAREKGVAITLEKVESLPEIM
                     RHGVLSTPGVVIDGRVVHAGGVPARSKVEGWL"
     gene            complement(879..>1885)
                     /locus_tag="PLL14_04275"
     CDS             complement(879..>1885)
                     /locus_tag="PLL14_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG03171.1"
                     /translation="WLAGLALGGVLWVAAYSHLGEFADAVIGAFGLSRRTPFGEAVHF
                     FFYDTPKVLLLLTGIVFVIGVVQTFFSPERTRALLSGRREGLGNVLAASLGIVTPFCS
                     CSAVPLFIGFLSAGVPLGVTFSFLISAPMVNEVALVLLFGMFGWQVAALYLGMGLLVA
                     IVAGLVIGRLKMERYLEDWVQQILSGQMAQAVGEQLSWLERLQVGAAHVREIVGKVWP
                     YVLAGIGLGAGIHGYVPEDFMAAFMGREVWWAVPAAVLLGVPMYTNAAGIIPIVEALI
                     GKGAALGTVLAFMMAVIALSAPEMIILRQALKPTLIATFVAVVATGILLVGYVFNWLL
                     "
     assembly_gap    1886..1985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1986..3309)
                     /locus_tag="PLL14_04280"
     CDS             complement(<1986..3309)
                     /locus_tag="PLL14_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03172.1"
                     /translation="MPPIHVIRDAVVAGSAERSPAVPWALASLALSMLLSSLGTSIAN
                     VGLPTLAQAFAASFQEVQWVVLAYLLAITTLIVGVGRIGDLIGRRRLLLAGIVLFSVA
                     SLLCGVASTLGQLIAARAAQGCGAAIMIALSMALVGEAVPQARTGSAMGLLGTMSAIG
                     TALGPSLGGALIAGFGWRAIFLVNVPLGLLTFLLARRHLPVDRRGPKTGRAGFDRLGM
                     LLLALTLAAYALAMTMARSRFGPLNIALLLVAAIGVGLFVRVEARATAPLIDLAIFRD
                     PGLRAGLAMSALVATVIMTTLVVGPFYLARALGLDAPLVGLVMSAGPLAAALSGVPAG
                     RSVDRSGAPRMTIVGLIGVAAGSCMLSMLPTRLGISGYLAPMIVITASYALFQTANNS
                     AVMSASRPEQRGVISGMLGLARNLGLITGASVLGAVFALAAATPDIATA"
     gene            3455..4339
                     /locus_tag="PLL14_04285"
                     /pseudo
     CDS             3455..4339
                     /locus_tag="PLL14_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017994112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4549..>4862)
                     /locus_tag="PLL14_04290"
     CDS             complement(4549..>4862)
                     /locus_tag="PLL14_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG03173.1"
                     /translation="DTMPYDASYRKEHIPGAQQFLFPVPRMSTWDTSETDGRTAADFA
                     ALLGADKARTLVFYCGFVKCTRSDNAAAWARKLGYTQVYRFPGGLFSWKGAGFPLEAV
                     Q"
BASE COUNT          839 a         1595 c         1600 g          728 t
ORIGIN      
        1 tcgctttggc tcacaagatg ttgcgcacca gcttcttcat gctcaagcgt ggcgactatt
       61 accgggactc cgccaccaac tatgagcagc tatcggtgca gcgcaacgct tctcgctgga
      121 tcaaggcttt gattcgcttc ggcttcatcc ccgcagccgc ctgattcacg accaccgatc
      181 tccatgaccg ctcgcggtca ggcacccgtg cgcttggaga ctgggttctt tcacattaat
      241 gaaacgggtg gccatcgttt ccttgagcgc atcgagtccg gcgcccttgc cgatggcctt
      301 ggcgagcgca tagcctttga gggcggcgat caggatgtcg ctgccgaggg ccatttcggt
      361 gtctttggcc ttcctgatca actgccgcag gcggctgaaa cgcgggtgcc gctgaacgta
      421 ggtggcgaga tcgtgttgcg cttcggtcag gtcgtaaccg ggcgggatga gttgcgcatt
      481 ctggccgagc agcatccgtg tctggcggca cagcgattcg gaccgctcgc ccgcctctgc
      541 gctcggccag cgcgcctgcg gatcagccgc ggcggtcgcc gcgcggcggc ttcacagcca
      601 gccttcgacc ttgctgcgcg ccggtacgcc gccggcatgg acgacgcggc cgtcgatgac
      661 cacgccgggc gtgctcagca cgccgtggcg catgatctcc ggcaggctct cgaccttttc
      721 gagggtgatc gccacgccct tttccctggc cactgcctcg atgacggcga ccgtcgcgcg
      781 gcagttggca cagccggtgc cgaggacctt gatgctctgc atggggtttc ccttcctggg
      841 gtcaatattc atcagtcgcg gctggtgcgg ccgcggcgtc acaacagcca gttgaaaacg
      901 tagcccacca gcaggatgcc ggtggccacc actgcaacga aggtggcgat cagggttggc
      961 ttcagcgcct ggcgcagaat gatcatctcc ggcgccgaca gcgcgatcac cgccatcatg
     1021 aacgcgagca cggtgcccag cgccgcgccc ttgccgatca gcgcttcgac gatcggaatg
     1081 atgcccgcgg cgttggtata catcggcacg ccgagcagca ccgcggccgg caccgcccac
     1141 cagacctcgc ggcccatgaa cgcggccatg aaatcctccg gcacatagcc gtggatgccg
     1201 gcgcccaggc cgatcccggc gagcacgtag ggccagacct tgccgacgat ctcgcgcaca
     1261 tgcgccgcgc cgacctgcag ccgttcgagc caggaaagct gctcgccaac cgcctgcgcc
     1321 atttgtccgg agaggatctg ctgcacccag tcctcgaggt agcgttccat cttcagccgg
     1381 ccgatcacca ggccggcgac gatggccacc agcagcccca tgccgaggta gagcgcggcc
     1441 acctgccagc cgaacatccc gaacagcagc accagcgcga cttcattgac catcggcgcg
     1501 gagatcagga acgagaaggt cacgccgagc ggtacgccgg ccgagaggaa gccgatgaac
     1561 agcggcaccg ccgagcacga gcagaacggc gtgacgatgc ccaggctggc cgccagcacg
     1621 ttgccaagac cttcgcgccg gccggaaagc agcgcccgcg tccgctccgg cgaaaagaag
     1681 gtctgcacga cgccgatgac gaaaacgatg ccggtcagca gcagcaacac cttcggcgtg
     1741 tcgtagaaga agaaatgcac ggcttcgccg aaaggcgtcc gccgggaaag tccgaaggcg
     1801 ccgatcaccg cgtcggcgaa ttcgccgaga tgactgtagg cggcgaccca cagcacgccg
     1861 cccagggcca gtccggccag ccagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnngcgcc gtggcgatgt ccggggtggc tgccgcgagc gcgaacaccg cgcccagaac
     2041 ggatgcgccg gtgatcagcc cgagattgcg cgccaggccg agcatcccgg aaatgacgcc
     2101 gcgctgctcc gggcggctgg cggacatcac ggcgctgttg ttggcggtct ggaacagggc
     2161 atagctggcg gtgatgacga tcatcggggc gaggtagccg gagatgccga gcctcgtcgg
     2221 cagcatcgag agcatgcagg aaccggccgc gacgccgatg agcccgacga tggtcatgcg
     2281 tggtgcgcca gaacggtcca cactgcgccc ggccggcacg ccgctcagcg cggcggcgag
     2341 tgggcccgcc gacatgacga gtccgacaag gggcgcatcg agcccgagcg cccgcgcgag
     2401 atagaacggc ccgaccacca gcgtcgtcat gatcaccgtc gccaccagcg cgctcatcgc
     2461 gaggccggca cgcaagccgg gatcgcggaa tatcgccagg tcgatcaagg gcgctgttgc
     2521 tctcgcctcg acccgcacga agaggccgac tccgatggcg gctaccagga gcagtgcgat
     2581 gttcagcgga ccgaagcggc tacgcgccat cgtcatcgcc agggcatagg ccgcgagcgt
     2641 cagggccaac agcagcatgc ccagacggtc aaagccggcc cgcccggtct tcggcccccg
     2701 gcgatccaca ggcaaatggc gacgcgcgag cagaaaagtc aggaggccca gcggtacgtt
     2761 gaccaggaag atcgcccgcc agccgaatcc ggcgatcaga gcgccgccga gcgacggacc
     2821 gagtgccgtg ccgatcgccg acatcgtgcc gagcagcccc atggcgctgc cggtcctggc
     2881 ctgtggaacc gcctcaccga ccagggccat gctgagcgcg atcatgatcg ccgcgccgca
     2941 gccctgcgcc gcacgcgcgg caatcagttg ccccagcgtc gaggcgacgc cgcagagcag
     3001 cgaggccacg ctgaacagga cgatgccggc caacagcagc cggcggcggc caatgaggtc
     3061 accgatccgg ccgacgccga cgatcagggt ggtgatcgcc aggagatagg cgaggacgac
     3121 ccactggacc tcctggaagg aggcggcgaa cgcctgggcc agggtcggca ggccgacatt
     3181 ggcgatactg gtgccgagcg aggacagcaa catcgacagg gcgaggctgg caagcgccca
     3241 gggaaccgcc ggactgcgtt ccgcgctgcc ggcgactacc gcgtcgcgga ttacatggat
     3301 cggtggcatg tgccctcctg ttctgcggac tcccgaagtg gagcgcttgg agagctactc
     3361 tatgcctttg cctggcctgg cggaaggcgc agcgtttgca ctttgtgcgt gcgtttgacg
     3421 ccgcatcacg atccggccgt gctatggtcg cggcatgtcg atgcccgatc tcaacctgtt
     3481 ggtcaccctt gatgtgctgc ttgcggaagg cagcgtggcg cgcgccgcgc agcggttacg
     3541 gctgagcccg tcggcgatga gccgggcgct gacgcgattg cgcgagacga ccggcgaccc
     3601 cctgttggtc cgggctggac gcggcctcgt tcctaccccg cgtgcgctcg aactgcgcgc
     3661 gcaggtcagc cggctggtgc aggaggccga agcggttcta cggccggcag aaaagctcga
     3721 cctcgaacgg ctgcagcgaa cattcacgct gcggaccagc gaaggctttg cggaaaactt
     3781 cgggccggac ctgattgcgc gcgtcgctga agaagcaccc ggcgtgcggc tctgcttcgt
     3841 gccgaagtca ggcagggaca gcgcgccgct gcgcgacggc agcgtcgatc tggagacggg
     3901 tgtcgtcagc cggacgacag ccccggaact gcgggtgcag gccttgttcc gggaccgctg
     3961 gatcggtgtg gtgcgcatgc gccatgcgct gagccagggc gagatcactc cccaacgcta
     4021 tgcggctggc agacacatct gcgtctcgcg ccgggggcta gtccaggggc cgatcgatga
     4081 agccctgaag ctgctggggc tggaacgcga ggtggtcacc atcgtcagcg gtttttcgac
     4141 agcgctggct ctcgcccggg cttccgacct gatcgccagt gtccccgagc ggcataccgg
     4201 cagccttcgc gccggcatgc atgctttttc cctgccgatc gccacgccgg cgatcaccgt
     4261 ctcattgcac tggcacccgc ggatggatgc cgatccggcg catcgttggc tgcgcggttg
     4321 cgtgcgggag gtttgcgcac cggtcgtcgc cgattcgtcg cggtatggag atgccgccaa
     4381 atgccggatt cgtctccgga ccgtggcggg agtttcggaa ggctgccggc gcggccattt
     4441 agaggccaat gcaggcctgt ccgcgcccgc tgaaaatagc ccgctgacgc ggccgaaggc
     4501 ggcccaggcc aaccgagagg acggcatcac caccgacccg gtgcagcgct actgcacggc
     4561 ttcgagcgga aaacccgcgc ccttccagga gaagagcccg ccgggaaagc gatagacctg
     4621 cgtatagccg agtttgcgtg cccatgccgc ggcgttgtcg ctgcgcgtgc atttgacgaa
     4681 gccacagtag aacaccagcg tcctggcctt gtcggcaccg agcaaggccg cgaaatcggc
     4741 cgccgtcctg ccgtcggttt cactggtgtc ccaggtactc attcgcggca ccgggaaaag
     4801 gaactgctgc gcacccggaa tgtgttcctt ccggtagctg gcgtcgtagg gcatggtgtc
     4861 ga
//
LOCUS       DAOSIT010000123         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15678_length_4821_cov_9.998741, whole genome shotgun sequence.
ACCESSION   DAOSIT010000123 DAOSIT010000000
VERSION     DAOSIT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15678_length_4821_cov_9.998741"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2327
                     /locus_tag="PLL14_04295"
     CDS             <1..>2327
                     /locus_tag="PLL14_04295"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOG03174.1"
                     /translation="YTLNPCREYWFEIVDRKRLSYLAARARAAHHEVGNALLAAWGKQ
                     TQAQIDLLLSDAGAIVEEDSLFLPAAGQHLLARVQNAILDLDELAPGSITLAADDRSI
                     EVHVCHSPTRELEVLHDQLLAELAGADPVTPDQILVLLPDLPAAAPLIDAVFGTAPAE
                     RRIPYQITGLPQIRINPFARVLDLLLTICSSRFTASAVFDLLQQPPVAARFALGNDEL
                     ECIRDWIHDAGIRWGLDAGSRSSEDLPPTDRNSFADGMHRLFLAYALGDELQARNTVI
                     AGRIAAGNPAGGDAATLGRAWRFLEALRELRDEWSQARDASAWQRSLGDALDRFTRAD
                     DDLVDDLRALQATIRELHHNMLRGGNRSPLPLAVVHQALTALLDDPSRGGVPGGGITF
                     ASLSSLRALPYRMVCVLGMSDGAFPSVNPQAEFDLMALRPQPGDRQRRLDERNLFLDL
                     LLAARQRLYLSYSGRNIRDNSIIPASVLLAELLDYVTTACAQDPADPASVRAVRRRLT
                     VEHPLQAFSPDYFLDDGDVRRRSFNAEYCQALRNALTSPAAQPAQPWSEDPDSDDESF
                     RDPSPVFFTRALVPPAAEWRQVNIDQLLEFFRNPCRTLLVRRLNMALTVAAQELQDDE
                     PFLPDYPGRQAFCRRLLPSVLAGAAESELLQLGLAGNEFPSGPLGERLIAEEVAQLQS
                     FATALGQQLAAAPLPAVQGHFEYPLADGTWQLSGVFGDLRPDGLLRYRYDDVRASDYL
                     AGWINHLFLCAVAPAGVQRQTTWHSRDGSYRLTPC"
     assembly_gap    2328..2427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2428..2781
                     /locus_tag="PLL14_04300"
     CDS             <2428..2781
                     /locus_tag="PLL14_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOG03175.1"
                     /translation="QRQTTWHSRDGSYRLTPCEPETARAHLSRLMEFYRRGLLAPLHF
                     FPKSAWAYVRHQSSLAAARARWTNNRNPAWGESADPAFRLALRGLANPLDGDFEHCAS
                     SILGPMFDYLEDPRS"
     gene            2778..>4821
                     /locus_tag="PLL14_04305"
     CDS             2778..>4821
                     /locus_tag="PLL14_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG03176.1"
                     /translation="MNTPTPSSTDEASDLDVFACPLDGRHLIEASAGTGKTWNICGLY
                     LRLLLEGDFTVQQILVVTFTNAATAELRERIRSRIVDVLGFLQRDIAASDPFAARLVA
                     AVESRGGGDRERMRACLDAALHTFDEAAVFTIHGFCQRALSDTPFAAGQPFKLELLHD
                     DQPLRLQAVSDFWRRHIAGGRTSPMLANHLATAKDSPESWARLLARRLARSHARLIWP
                     EGMCSDPQQLDDNALTSAFELARRLWGGDGARPTATLLEGLAALNANSYKAESIRVAA
                     RSWNSWLAGDDALHAPDPEAGKTRLFSAEFIAGKTRKNQTPPQHPFFAAAAELLAAHA
                     STTRALRLARLSLLRQMFDEAGAGLRAQKRRQRLLSFDDILNDVEVALTSGKAPWLAG
                     SLRSRYPVALIDEFQDTDPVQFAIFNAIYGSTAELPGGSLFLVGDPKQAIYSFRNADL
                     QTYLAARRQTGRPYTLRHNQRSSPALIEACNRLFAINPEAFILEGLRYVPVTLGDKPR
                     VQLIDRGEESAAALRIWQLPGDGQHLLRAQALHAVVLATAGEVARLLRAAQAGLITIG
                     ERSLVPGDIAILVRSHTQGRLTRDALLQFGVGCVELSQQNIFHTPDADDLERVLLAIL
                     EPARPPLLYSALATELMGFDADAVATLAGQETHLLQTMSRFSAYRESWLRRGFGVMF"
BASE COUNT          799 a         1717 c         1430 g          775 t
ORIGIN      
        1 ctacacgctc aatccgtgcc gcgagtactg gttcgagatt gtcgaccgca agcgcctgag
       61 ctacctcgcg gcacgcgccc gtgccgcgca tcacgaggtc ggcaacgcgc tgcttgccgc
      121 ctggggaaag cagacccagg cgcaaatcga cctgctgctc tctgatgccg gcgcgatcgt
      181 cgaggaagac agtctgttcc tgccggcggc cggccagcac ctgctggctc gcgtccagaa
      241 tgccatcctc gacctcgatg aactcgcacc gggtagtatc acgctggccg ccgacgaccg
      301 cagcatcgaa gtgcatgtct gccactcgcc gacgcgcgaa ctggaagtcc tgcatgacca
      361 gttgttggca gagcttgcgg gcgcagaccc ggtgaccccg gaccagatcc tggtgctgct
      421 tcccgacctc cctgcagcgg caccgttgat cgacgcggtg tttggcacgg cgccggcgga
      481 acgccgcatt ccttaccaga tcacgggcct gccgcagatc aggatcaacc cgttcgcccg
      541 cgtgctcgat ctactgctca ccatctgcag cagccgcttc accgccagcg ccgtcttcga
      601 tctgctgcaa cagccgccgg tggcggccag gttcgccctt ggcaacgacg aactggagtg
      661 catccgtgac tggatccacg acgccggcat ccgctgggga ctcgatgccg gtagccgcag
      721 cagtgaagac ctgccgccaa ccgaccgcaa cagctttgcc gacggcatgc accgattgtt
      781 cctcgcttac gccctcggcg atgaactgca ggcgcgcaat accgtcatcg ccggacgcat
      841 cgccgccggc aatcccgcag gcggcgacgc cgccacgctg ggccgcgcct ggcgcttcct
      901 ggaagcactg cgggaactgc gtgacgagtg gtcgcaagcg cgcgacgcca gcgcctggca
      961 acgctcgctc ggtgacgcac tggaccgctt cacccgcgcc gacgacgatt tggtcgacga
     1021 tctgcgcgcg ctgcaggcga cgatcaggga actgcaccac aacatgctgc gcggcggcaa
     1081 tcgctcgccg ctgccgctgg cggtggtcca ccaggcactg accgccttgc tcgacgaccc
     1141 cagtcgcggc ggcgtccctg gcggcggcat cacctttgcc tcgctgagca gcctgcgcgc
     1201 cctgccctat cggatggtct gcgtgctcgg aatgagcgat ggcgcatttc cgagcgtcaa
     1261 tccacaggcc gagttcgacc tgatggccct gcggccgcag ccgggcgacc ggcaacgccg
     1321 ccttgacgag cgcaacctgt ttctcgacct gctgctcgct gcccggcaac gcctgtatct
     1381 gagctacagc gggcgcaaca ttcgtgacaa cagcatcatt ccggcgtcgg tgctgctcgc
     1441 cgaattgctc gattacgtca ctaccgcctg tgcgcaggac cctgccgatc ccgccagcgt
     1501 gcgtgccgtg cggcgccggc tgacggtcga gcatccgctg caggccttct ccccggacta
     1561 cttcctggac gacggcgatg tccgccgccg cagcttcaac gccgaatact gccaggccct
     1621 gcggaatgcg ctgacctcgc ccgctgcgca gcccgcgcaa ccctggtcag aggatccgga
     1681 cagtgacgat gaaagcttcc gcgaccccag ccctgttttc tttacccgcg cgctggtacc
     1741 gcccgcagcg gaatggcgtc aggtcaacat cgatcaactg ctggagtttt tcaggaaccc
     1801 ttgccgaacc ctgctcgtca ggcggctgaa catggcactg acggtcgctg cccaggaatt
     1861 gcaggacgac gagccatttc tgcccgacta ccctggtcgt caggcgttct gccggcgcct
     1921 cctgcccagc gtgctggccg gtgcagcaga gagcgaactc ctgcaactcg gcctggcggg
     1981 caacgagttc ccctccggtc cgctgggcga gcggctgatc gccgaagaag tagcgcaact
     2041 gcagagcttc gccacggcgc tcggccagca actcgcagcc gcccccttgc cggcagtaca
     2101 gggccatttc gagtacccgc tggccgacgg aacctggcaa ctcagcggcg tgttcggcga
     2161 cctgcgcccg gacggactgc tgcgatatcg ctacgacgac gtgcgcgcgt ccgattacct
     2221 cgccggctgg atcaaccact tgttcctttg cgccgtcgca cccgccggtg tccagcggca
     2281 aaccacctgg cactcgcgcg acggcagcta ccgcctgacc ccctgtgnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnncag cggcaaacca cctggcactc gcgcgacggc
     2461 agctaccgcc tgaccccctg tgaaccggag accgcccgtg cccacctcag ccggctgatg
     2521 gagttttacc gacgcggcct gttggcgccg ctgcacttct tcccgaaaag cgcctgggcg
     2581 tacgtcaggc atcaaagcag tcttgccgcc gcacgtgccc gctggaccaa caaccgcaat
     2641 ccggcctggg gcgagtccgc cgatccggca ttccggctgg cgctgcgcgg cctcgccaac
     2701 ccgcttgacg gcgatttcga acactgcgcg agcagcatcc tcggtcccat gttcgattac
     2761 ctcgaagacc cccggtcatg aacacgccga caccgtccag cacagacgaa gcgagcgacc
     2821 tcgacgtatt cgcctgccca ctcgacggcc ggcatctgat cgaagcgtcc gccggtaccg
     2881 gcaagacctg gaacatctgt ggcctgtacc tgcgcctgct gctcgaaggc gatttcaccg
     2941 tccagcagat cctggtggtg acgtttacca atgccgccac cgccgagttg cgcgagcgta
     3001 tccgcagccg gatcgtcgat gtcctcggct ttctgcagcg cgacatcgcc gcgagtgacc
     3061 cgttcgccgc ccgtctggtc gctgccgtcg agagccgtgg cggaggggat cgtgagcgca
     3121 tgcgcgcctg cctcgacgcg gcgctgcaca ccttcgacga ggccgcggta ttcaccattc
     3181 acggcttctg tcaacgcgcc ctgtcggata ccccgtttgc cgccgggcag ccgttcaagc
     3241 tcgaattgct gcacgatgac cagccgctgc ggctgcaggc agtcagcgat ttctggcgac
     3301 gacatatcgc cggcgggcgt acctcgccga tgctcgccaa ccacctggcc accgccaagg
     3361 attcgccgga aagctgggcc aggctgctcg cgcgccggct ggccaggtcg cacgcgcgcc
     3421 tcatctggcc ggaaggcatg tgcagcgacc cgcaacagct cgacgacaac gccctgacca
     3481 gcgccttcga actggcgcgg cggctatggg gaggcgatgg cgcccggccc accgcaacct
     3541 tgctggaggg tctggcggcg ctgaacgcca actcgtacaa ggcggaaagt atccgggtgg
     3601 cggccaggag ctggaacagc tggctcgccg gcgacgatgc cctgcacgct cccgatcccg
     3661 aagccggcaa gacacgactg ttttcggccg agttcatcgc cggcaagacc aggaagaacc
     3721 agacgccgcc gcaacatcct ttcttcgccg ccgccgctga actgctggcg gcgcacgcgt
     3781 cgacaacacg agcgctgcgg ctcgcgcggc tatccctgct gcggcagatg ttcgacgaag
     3841 ccggtgccgg cctgcgcgca cagaaacgcc gccagcgcct gctgtccttc gacgacatac
     3901 tcaacgacgt cgaagtcgct ttgacgtcgg gcaaagcccc ctggctcgcc ggatcactgc
     3961 gcagccgtta cccggtagcg ctgatcgacg agttccagga taccgacccc gtgcagttcg
     4021 ccattttcaa cgccatctac ggcagcacgg ctgaactgcc cggtggctca ctgttcctcg
     4081 tcggcgatcc caagcaggcc atctacagct tccgcaacgc cgacctgcag acctacctcg
     4141 ccgcacgacg tcagacgggc agaccctaca cgctgcgcca taaccaacgt tcgagcccgg
     4201 ccctgatcga agcctgcaat cgcctgttcg cgatcaatcc cgaggccttc atcctcgaag
     4261 ggctgcgcta cgtcccggtg accctcggtg acaagccgcg ggtgcaattg atcgaccgcg
     4321 gtgaggagag cgcggcggcg ctgcgcatct ggcaactgcc cggcgacggc cagcacctgc
     4381 tgcgcgcgca agcgctgcat gcggtcgtgc tggccaccgc cggcgaggtc gcgcgcctcc
     4441 tgcgcgcggc gcaggctggc ctgatcacga tcggcgagcg atcactagtc ccgggcgaca
     4501 tcgccatcct ggtcaggagt cacactcagg gccgcctgac cagggacgcc ctgctgcaat
     4561 tcggcgtcgg ctgcgtcgaa ctgtcacaac aaaacatctt ccatactccc gacgccgacg
     4621 atctcgaacg ggtgctgctg gccattctcg aacccgctcg gccaccactg ctctacagtg
     4681 ccttggcgac cgaactgatg ggcttcgacg ccgacgccgt cgcgacactg gcaggacagg
     4741 aaacacacct gctgcagacc atgtcacgtt tcagcgccta ccgcgagagc tggctacggc
     4801 gcggcttcgg cgtcatgttc c
//
LOCUS       DAOSIT010000124         4779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15856_length_4779_cov_9.076207, whole genome shotgun sequence.
ACCESSION   DAOSIT010000124 DAOSIT010000000
VERSION     DAOSIT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15856_length_4779_cov_9.076207"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="pglZ"
                     /locus_tag="PLL14_04310"
     CDS             complement(<1..1325)
                     /gene="pglZ"
                     /locus_tag="PLL14_04310"
                     /inference="COORDINATES: protein motif:HMM:NF033446.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system phosphatase PglZ"
                     /protein_id="HOG03177.1"
                     /translation="MSLPQIEAQALAVLECVGDARVIAIQARTQAAWPEAITLAGRPF
                     RLRWCRSPLMVRAALSALEEETAEPAGAGLILLTPLAARELGCDVVARLARAQVFQPD
                     AWGMVQQLFRAREIDARLNRCRWMAQLLVERASMGVYPPVPNGFLDMDTAWRNVLGRC
                     LALDAARPDATMLLHWSLGADSAGLFADLPDPAKPPIVAWLSDSAGAVGTLVMRCVLA
                     GNTQDAVPLGLVCGVLLSAQGEGVHDLAAAAIRLERFIGNQRIGVSEGRLWAADSASG
                     LKTVTPARRHELIERAEWLLRELQVDAYAHLSDVLPLGLEQRLGLLAEAFRAFIGEPS
                     SARLQVIEGAADSVARHAHADPAGTRLLRVKMAVRLCRWLLADRSASQGRAGRPASGF
                     ADLAHRYAGEGAFVDWARCKLIGGDDLPGLSSAFVALRAAARQQADAFNR"
     gene            complement(1343..4318)
                     /locus_tag="PLL14_04315"
     CDS             complement(1343..4318)
                     /locus_tag="PLL14_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HOG03178.1"
                     /translation="MPLDDGLSIMSRHAHALGYDAVILFLDELILWLASRAADVSFVS
                     SEGTKLVKLVEAGNADRPIPLVSFVARQRDLRELVGENLAGSLQLQFSDVLRHWEARF
                     HRITLEDRNLPAIAEKRVLRPTSEAARQTLGNAFDEFQKVRPEVWNTLLTETADKAMF
                     RKVYPFSPALVQTLIAVSAALQRERTALKLMLQLLVDRRHDLELGQIIPVGDLFDVIA
                     DGDEPFSEAMRIHFDNAKRLYRQKLLPHLERQHGVTWENIRIGDADPARARALRNDAR
                     LIKTLLLSALVPEVDSLKALTAQRLAALNHGTIRSPIPGRETQDVLRKCKEWAADIGE
                     IRVTDDTNALISIQVTGVDIEPIVRGPAASSDNPGNRRRRVREMLFEQLGVTDSNDLF
                     ATYAVLWRGTKREVEVLFENVRELSDEKLRGRPGTWTVVIDFPFDDPRFSPADDLARL
                     AGYRGGDTQTLVWLPSFFSAKAQHDLGRLVVLDYILTGERFNELAAHLALVDRGPAQA
                     LLRNQRDQLQQRVRQYLEVAYGIAGDSRDAVVNPMAPEDQFRSLDQTLTPLPPVGANL
                     KSAFEALLDQLFRHQFPAHPVFNADVRPAVVKKVWSELERAIGSADERAPIGDRVLRQ
                     LVRSIVDPVQLGKTGESHLVLLDHWRSHFLREQAKTGAAFTVGNLRQWIDQPLAMGLP
                     EEVQNLIILAFAGHTNRRFVRGNVPVSPGIDQMPDDLELREQTLPEPAAWESACKRAA
                     ALFGLTIPTSRNAGNVVRLFEEVQARAREAREPIGSLLRALNEKAALFPAAGDNHRLQ
                     TARSALALLADLLNAEGAAVVTTLAGATIETSEVAVRQTLARARELDEAVRTGAWDIF
                     EAMKTLTDERQAAARAILARVSETLAADEHAIGLQAALAEQRSKAVRLLTVAPPPAPR
                     PSTGGAVTPPLPPKAPPPLSPGRPAPKPPVIVQESAATGLASPQALALLDELHARLDR
                     DMDLLLSIAWRLEKPGSSQ"
     gene            4280..>4779
                     /locus_tag="PLL14_04320"
     CDS             4280..>4779
                     /locus_tag="PLL14_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03179.1"
                     /translation="MAAHDAQAIVERHESLAAALGDVGNRREVAADQVADQADAVVAA
                     RRFEHCRLEARGIPAAAQVAPAASVLAAGVPGVASVTLLVQITEEGRIAETTAHAYRI
                     LEQTLRQVEARNRCLRMPLAHELGVAAENRRLHAARTDHVIRHEQEFAVLRPAVVLGD
                     DVGQLG"
BASE COUNT          767 a         1691 c         1592 g          729 t
ORIGIN      
        1 cgattgaagg catcggcctg ctggcgcgcc gccgcacgca gagcgacgaa agccgatgaa
       61 aggccaggca ggtcgtcgcc accaatcagc ttgcagcgcg cccaatcgac gaaagcgcct
      121 tcgccagcgt accggtgcgc gaggtcggcg aacccggatg ccggacggcc ggctcggccc
      181 tggctcgccg accggtcggc cagcagccat cgacacaggc gaaccgccat cttcacccgc
      241 agcaggcgcg tgcccgccgg gtcggcatgc gcatggcgcg ccaccgaatc ggccgcccct
      301 tcgatgacct gcagccgtgc tgaagacggc tcgccgatga aggccctgaa cgcctccgcg
      361 agcagcccaa gccgctgctc caggccgagc ggcagcacgt cgctcagatg ggcgtaggca
      421 tcgacctgga gttcgcgcag cagccactcg gcacgttcga tcagctcgtg acgccgggcg
      481 ggcgtcacgg tcttcagacc gcttgccgaa tcagcagccc acaggcgtcc ctcactgacg
      541 ccgatgcgct ggttgccgat gaagcgttcc agacgaatcg ccgcggcggc gaggtcgtgc
      601 acgccttcac cctgagccga caacagcacg ccacagacca ggccgagcgg gacggcatcc
      661 tgcgtgttgc cggccagcac gcagcgcatc accagcgtgc ccacggcgcc ggccgagtcg
      721 ctcagccagg cgacgatcgg cggtttggct ggatccggca gatcagcgaa aagccccgcc
      781 gaatcggcgc ccagtgacca gtgcaagagc atcgtcgcat ccggcctcgc cgcgtccagg
      841 gccagacaac ggccgaggac gttgcgccac gccgtgtcca tgtccaggaa accgttgggc
      901 accggcggat agacccccat gctcgcccgc tcgaccagca gttgggccat ccagcggcag
      961 cggttgaggc gcgcatcgat ctcgcgcgcc ctgaagagtt gctgcaccat cccccaggcg
     1021 tcgggctgaa aaacctgcgc gcgcgccagc cgggcgacga cgtcacagcc cagttcgcga
     1081 gcggccagcg gcgtcagcag gatcagcccc gcgcccgcag gctcggcggt ttcttcttcc
     1141 agggcagaga gtgccgcgcg gaccatcagc gggcttcgac accagcgcag ccggaacggc
     1201 cggcccgcca gggtgatggc ttccggccag gcggcctgcg tcctggcctg aatcgcaatg
     1261 acccgcgcgt cgccgacgca ctccagcacc gcgagcgcct gcgcttcgat ctgcggcagg
     1321 gacaaaggcc cggccgtacc ggtcactggc tgctccccgg cttttcgagc cgccacgcga
     1381 tcgacagcag caggtccatg tcgcggtcca gcctggcgtg cagctcgtcg agcagcgcca
     1441 gcgcctgtgg gcttgcaagg ccggtggcgg ccgattcctg cacgatcacc ggcggcttgg
     1501 gtgccggccg gccgggggac aacggcggcg gcgctttcgg gggcagcggt ggcgtcaccg
     1561 ctcctcccgt tgacgggcgt ggtgccggtg gtggcgctac cgtcagcaag cgcacagcct
     1621 tgctgcgctg ctcagcgagc gcggcctgca gcccgatcgc atgctcgtcg gcggccagcg
     1681 tctcactcac ccgagcgagg atcgcgcgcg ccgcggcttg ccgctcgtcg gtcagcgtct
     1741 tcatcgcctc gaagatatcc cacgcgccgg tgcgcaccgc ttcatcgagt tcacgcgccc
     1801 tggccagggt ctggcgcacg gcaacctcgg aagtctcgat cgtcgcaccg gcgagcgtcg
     1861 tgacgacagc cgcaccctcg gcattcaaca ggtccgccag cagggccagc gcggaacgcg
     1921 ccgtttgcag ccgatggttg tcgccggcgg ccgggaacag ggcggctttc tcgttcagcg
     1981 ccctgagcag gctgccgatc ggctcgcgcg cctcgcgcgc cctggcctga acctcttcaa
     2041 acagcctgac gacgttgccc gcgttacgcg aggttggaat cgtcaagccg aacagcgcgg
     2101 cggcgcgctt gcaggcggac tcccacgccg cgggctcggg caaggtctgc tcgcgcaact
     2161 ccaggtcgtc cggcatctgg tcgatgcctg gcgagacggg gacattgccg cgcacgaagc
     2221 ggcggttcgt atgcccggcg aaggcgagga tgatcaggtt ctgcacctcc tctggcagcc
     2281 ccatggccag cggttgatcg atccactgac gcagattgcc gaccgtgaat gcggcgcccg
     2341 ttttggcctg ttcgcgcagg aagtgcgagc gccaatggtc aagcagcact aaatggctct
     2401 cgccggtctt gcccaactgc accgggtcga cgatcgagcg gaccagctgc cgcagcaccc
     2461 ggtcgccgat cggcgcacgt tcgtcggcgc tgccgatggc gcgctcgagt tccgaccaca
     2521 cctttttcac caccgccggc ctgacatcgg cattgaagac cgggtgcgcc ggaaactggt
     2581 gccggaagag ctggtcgagc agggcctcaa aagccgattt caggttcgct cccaccggcg
     2641 gcagcggcgt cagcgtctgg tccagcgagc ggaactggtc ttcgggcgcc atcggattca
     2701 ccacggcgtc gcgcgagtcg ccggcaatgc cgtaggccac ctccaggtac tggcgaaccc
     2761 gctgctgcaa ctggtcacgc tggttgcgca gcagcgcctg cgccggcccg cggtcgacca
     2821 gcgccagatg agcggccagc tcgttgaagc gctcgccggt gaggatgtag tcgagcacca
     2881 ccaggcggcc caggtcatgc tgtgccttgg cgctgaagaa cgacggcagc cacaccagcg
     2941 tctgcgtatc gccgccgcga tagcccgcca gacgggcaag atcgtcggcc ggggagaagc
     3001 gcgggtcgtc gaaagggaag tcgatgacca ccgtccaggt gcccgggcgc ccgcgcagct
     3061 tttcgtccga cagctcgcgg acgttctcga agagcacctc gacctcgcgc ttcgtgccgc
     3121 gccacagcac cgcataggtc gcgaagagat cgttgctgtc ggtaacgccg agctgctcga
     3181 agagcatctc gcgcacgcgg cggcgccggt tgccggggtt gtcgctggag gcagccgggc
     3241 cgcgcacgat cggctcgata tcgacgccgg tgacctggat cgagatcagc gcattcgtgt
     3301 catcggtgac cctgatctcg ccgatgtcgg ccgcccattc cttgcacttg cgcaacacgt
     3361 cctgcgtttc gcggccgggg atcggcgagc ggatcgtgcc gtggttgagc gccgccaggc
     3421 gttgcgccgt cagcgccttc aacgaatcga cctcgggcac cagcgcgctc agcagcagcg
     3481 tcttgatcaa gcgcgcatcg ttcctcagcg cgcgcgcccg cgccgggtcg gcgtcgccga
     3541 tgcggatgtt ctcccaggtc acgccatgct gccgctccag gtgcggcagc agcttctgcc
     3601 ggtagagccg cttggcgttg tcgaagtgga tgcgcatcgc ctccgagaac ggctcgtcgc
     3661 cgtcggcgat cacgtcgaac aggtcgccga ccgggatgat ctggcccagt tccaggtcat
     3721 ggcgccggtc caccagcagt tgcagcatca gcttcaaggc cgtgcgctcg cgctgcagcg
     3781 ccgccgagac ggcaatcagc gtctgcacca gcgccgggct gaacggatag accttgcgga
     3841 acattgcctt gtccgcggtc tcggtgagca gcgtgttcca cacctcgggc ctgaccttct
     3901 ggaactcgtc gaaggcattg cccagcgtct gccgcgccgc ttccgacgtc ggccgcagca
     3961 cccgcttctc ggcaattgcc ggcaggttgc ggtcttcgag cgtgatgcgg tggaagcgcg
     4021 cctcccagtg gcgcagcaca tcgctgaact gcaactgcag cgacccggcc aggttctcgc
     4081 cgaccagttc gcgcaggtca cgttgtcgcg caacaaagct gaccagcgga atcggccgat
     4141 cggcgttgcc ggcctcgacc agcttgacca gcttggtgcc ctcgctgctg acgaagctca
     4201 cgtcggccgc ccggctggcc agccacagga tcagctcgtc gaggaacagg atcaccgcgt
     4261 cgtagccgag cgcgtgagcg tggcggctca tgatgctcag gccatcgtcg agcggcacga
     4321 aagcctcgcc gcggctctcg gcgacgtcgg caatcgccgt gaagtagcgg ctgatcaggt
     4381 cgccgaccag gcggacgcgg tcgtcgccgc tcggcggttc gagcattgcc gcctcgaagc
     4441 tcgcggcatc ccagccgccg ctcaggtcgc cccagccgct agcgtcctcg ccgccggcgt
     4501 ccccggcgtc gccagtgtca cactcctggt tcagatcacg gaagaaggtc gcatcgccga
     4561 gacgactgcg catgcgtacc gcatcctcga acagacgctc cgacaggtag aagcccggaa
     4621 tcggtgcctc cggatgccgc tggcgcacga actcggcgta gccgccgaga atcgccgact
     4681 ccatgctgcg cgcaccgatc atgtgatacg gcacgagcag gaatttgcgg tcctgcgtcc
     4741 agccgttgtg ctgggcgacg acgttggcca gctcgggca
//
LOCUS       DAOSIT010000125         4770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15894_length_4770_cov_9.759491, whole genome shotgun sequence.
ACCESSION   DAOSIT010000125 DAOSIT010000000
VERSION     DAOSIT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15894_length_4770_cov_9.759491"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLL14_04325"
     CDS             complement(<1..323)
                     /locus_tag="PLL14_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOG03180.1"
                     /translation="MAKRDFYDILGVNRDASDDDIKKAYRKLAMKYHPDRNPDNPRAE
                     EHFKEVKEAYEILTDTQKRAAYDQYGHAGIDPQAGMGAAGAGGFSDAFGGIFDEIFGG
                     GRGGRS"
     gene            complement(390..2327)
                     /gene="dnaK"
                     /locus_tag="PLL14_04330"
     CDS             complement(390..2327)
                     /gene="dnaK"
                     /locus_tag="PLL14_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOG03181.1"
                     /translation="MGKIIGIDLGTTNSCVAVMENGKPKVIENSEGARTTPSIVAYAE
                     DGEILCGAPAKRQAVTNPKNTLYAVKRLIGRRFEEKEVQKDISLMPFKILKADNGDAW
                     VEVRGKKIAPPQVSAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKKQGDKKIAVYDLGGGTFDISIIEIAEVEGEHQFEV
                     LSTNGDTFLGGEDFDQRLIDYIIEEFKKESGVNLKADVLALQRLKDAAEKAKIELSSG
                     QQNEINLPYITADSSGPKHLTLKITRAKFESLVDELIERTIEPCRIALKDAGCKIGDI
                     NDVILVGGQSRMPKVQERVKEFFGREPRRDVNPDEAVAVGAAIQGGVLQGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIKKNTTIPTKATQVFSTADDNQSAVTIHVLQGEREMASG
                     NKSLGQFNLSDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGRENKIKIQASSGLS
                     EAEVQRMVQDAEVHAEEDRKAHELAETRNQCDGMIHTVKKSLAEYGKELSDSEKSVIE
                     KAMREAEEAIRGSDLETIKAKSEALGTAAQKLGEKMYAKQQAHAEGAESAAGSAGTAG
                     EGKKDDSDVVDAEFTEVKGKK"
     gene            complement(2424..2984)
                     /gene="grpE"
                     /locus_tag="PLL14_04335"
     CDS             complement(2424..2984)
                     /gene="grpE"
                     /locus_tag="PLL14_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOG03182.1"
                     /translation="MQANEKESEPATAETATAESLEAAPAAALAPDVGPALEEALRQA
                     QTKADEYHDAWLRAKAETENVRRRAQEDIAKAAKFSIDRFARELLAVKDSLEAALASE
                     TSSVENLRSGTELTLKQLVAAFEKSTLAEINPLGEKFDPHRHQAISVVASEQEPNTVV
                     TVLQKGYLLADRVLRPALVVVAKGTT"
     gene            3162..4511
                     /locus_tag="PLL14_04340"
     CDS             3162..4511
                     /locus_tag="PLL14_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG03183.1"
                     /translation="MMEKIGKYEIIRSLGRGATAIVYLAWDPDLGRQVAIKLIHFGEN
                     NAAMSRRLRKLFQTEDAIGRRLDHPNIVRIYDAVVEAEQAYLVMEFVDGPALDKFCAI
                     NRLLPMHRVIGIIFKCCLALDHAFRRGVVHRDIKPANILIDANDNPKITDFGLGLNLQ
                     KDMGKDSTFVMGVGSPAYMSPEQVKNYPLNHKTDLYSLGVLLFQLLTGRLPFRANNAA
                     ALVYKIVNMDAPSVCALRPSLPPGLDPIIRKALEKDLYSRYRNGAEFAKDLTPVRYQM
                     QEEDQTQQDLTHFERLRNLTFFAEFEDIELWEVLRISVWRELSEKVTVVREGDNHRKF
                     GVIVDGFVEISSNGRVICRLGPSEVVGEMAFLHPTDSQRHATVVTLEPTLFLEINSAA
                     LELSSDEVRERFNKVLLVKVLGRLREANKVLAKLGQTAVQGSFARSSLGLTPPLELL"
BASE COUNT          870 a         1441 c         1348 g         1111 t
ORIGIN      
        1 gaacgtccac cacgaccacc gccgaaaatc tcgtcaaaga tgccgccaaa ggcgtcggag
       61 aagcctcctg ccccggcagc acccatgccc gcctgcgggt caatcccagc atgtccgtac
      121 tggtcatagg ccgctcgttt ctgcgtatcg gtaagaatct cgtaggcttc cttgacttcc
      181 ttgaagtgct cttcggccct gggattgtcc ggattgcggt ccggatggta tttcatcgcc
      241 agcttgcggt aggccttctt tatgtcatcg tcggatgcat cacggttgac gcccagaatg
      301 tcgtaaaagt cgcgttttgc cataggttct tgagcacttt gaggggcgaa gcgaggcgcg
      361 attatacgcc tcgcgtttca ggcggatgcc tacttcttgc cttttacttc ggtaaattcg
      421 gcgtcgacga catcactgtc atccttcttc ccctcgccgg cggtaccggc actgccggcg
      481 gcactctcgg caccctcggc gtgcgcttgc tgcttggcgt acatcttctc gccaagcttc
      541 tgcgcggccg tcccgagcgc ttcactcttg gccttgatgg tttcaaggtc actgccacga
      601 atcgcttcct cggcttcccg catcgctttc tcgatgacgc ttttctcgct gtcggaaagc
      661 tccttgccgt attcggcgag tgacttcttg accgtatgaa tcataccgtc gcactgattg
      721 cgcgtttccg ccagctcgtg agctttccgg tcctcttccg cgtgcacctc ggcatcctgg
      781 accatgcgct gcacttccgc ctcgctgaga cccgaggaag cctggatctt gatcttgttt
      841 tccctgccgg tggccttgtc cttcgccgag acatgcagaa tcccattggc gtcgatgtcg
      901 aaagtgactt cgatctgcgg cataccgcgc ggcgccggcg gaatatcgga gagattgaac
      961 tgcccgaggc tcttgttccc cgaagccatt tctcgctcac cctgcaatac atgaatggtt
     1021 accgcagact ggttgtcgtc cgccgtcgag aaaacctgcg tcgccttggt cggaatggtc
     1081 gtgttcttct tgatcagctt ggtcatcacc ccgccgagag tctcgatacc cagggacagc
     1141 ggggtcacgt cgagcaacaa cacgtccttg acttcgcctt gcagtacgcc tccctggatc
     1201 gccgcgccga cggcgaccgc ttcgtcggga ttgacatcgc ggcggggctc gcggccgaag
     1261 aactccttga cgcgctcctg caccttgggc atccgtgact ggccaccgac cagaatcacg
     1321 tcgttgatgt caccgatctt gcatccggca tccttcagcg cgatgcggca aggctcgatg
     1381 gtacgctcga tgagttcgtc gaccagcgat tcgaacttgg cgcgtgtgat cttcagcgtc
     1441 aggtgcttgg gtccggaact gtcggcagtg atgtacggca ggttgatctc gttttgctgc
     1501 cccgacgaca gttcgatctt ggccttttcg gcagcgtcct tgaggcgctg cagggccagc
     1561 acgtcggcct tcaggttaac cccggattcc ttcttgaatt cttcgatgat gtagtcgatc
     1621 aagcgctgat cgaagtcttc gccaccgagg aaggtatcgc cgttggttga cagcacctcg
     1681 aactgatgct cgccttcgac ctcggcaatt tcgatgatgg aaatgtcgaa ggtcccgcca
     1741 ccaagatcat aaacggcgat cttcttgtcg ccctgcttct tgtccatgcc gaaggccagt
     1801 gccgccgccg tcggttcgtt gatgatacgt ttcacttcaa ggccggcgat ccggccggca
     1861 tccttggtcg cctgccgctg gctatcgttg aagtaggccg gcacggtgat gaccgcctcg
     1921 gtgacttcct cgccaagata gtcctcggcg gtcttcttca tcttgcgcag gacctcggcg
     1981 gacacctgcg ggggggcaat cttcttgcct cgcacctcga cccaggcgtc gccgttgtct
     2041 gccttgagaa tcttgaaggg catcagcgag atgtcctttt gtacttcctt ttcctcgaag
     2101 cgacgaccga tgagccgctt gaccgcgtac agcgtattct tcggattggt caccgcctga
     2161 cgcttggctg gcgccccaca gagaatctcg ccgtcttcgg cgtaggccac gatcgatggc
     2221 gtggtacgcg ctccctcaga gttctcgatc acttttggct tgccgttctc cattaccgcg
     2281 acacaggagt tggtcgtacc gagatcaatg ccgatgatct tgcccatttc agaatccttt
     2341 caatatctca ttattttgta tatacattcg ccaaaaccgg aaggcctggt gatgaaccac
     2401 aactggggat catgccgaag atttcaagtt gtccccttgg ccactacgac cagcgccgga
     2461 cgcagcacgc gatcggccag cagatagccc ttttgcagta cggtgaccac ggtattgggt
     2521 tcctgctccg atgcgacaac gctgattgcc tgatggcgat gcgggtcgaa cttctcgcca
     2581 agcggattga tttcggctag tgtggatttt tcgaaagcgg cgaccagttg cttgagggtc
     2641 agttcagtac ccgaccgaag attctcgaca ctcgacgtct cgctggccag ggccgcttca
     2701 aggctgtcct tgacggccag caactcgcga gcgaaacgat caatggagaa tttggcggcc
     2761 ttggcgatgt cttcctgagc gcggcgacgc acattctcgg tctcggcctt ggcgcgcagc
     2821 catgcatcat ggtactcatc agccttcgtt tgcgcttgcc gcagcgcttc ttcgagggcg
     2881 ggcccgacgt ctggcgccag cgcggcggcg ggggctgctt ctaagctttc ggccgtggct
     2941 gtttcggcag ttgccggttc ggattctttt tcgtttgctt gcatagatgg tgatctcccg
     3001 gaaaacgcaa agaagctggg ggcgacttgc ccgatttcaa gcctttttgt agtctcgatg
     3061 ggcttgaaca gcgccgcacg ccgcgcataa tttttcatcc acgggcactt ctgctgctag
     3121 catttcaatt cccctgatca tctatagaac gcggtaccgg catgatggag aagatcggca
     3181 aatatgaaat cattcgctcc ctcggccggg gagccactgc gatcgtctat ctcgcgtggg
     3241 atcctgacct tgggcgccag gtcgccatca agctgatcca cttcggcgag aacaatgccg
     3301 ccatgtcacg gcgcctgagg aagctcttcc agaccgagga tgcgatcggt cggagactcg
     3361 accacccgaa catcgtcagg atctacgacg ccgtggtcga agcggaacag gcctatctgg
     3421 tgatggaatt cgtcgacggg ccggcgctcg acaagttttg cgcgatcaat cgactattgc
     3481 cgatgcaccg cgtcatcggc atcattttca agtgttgcct ggcccttgat cacgccttcc
     3541 ggcgtggggt cgtgcatcgc gacatcaagc cggccaatat tctgatcgat gccaacgaca
     3601 atccgaaaat caccgatttc ggcctcggcc tcaatctaca aaaggatatg ggcaaggact
     3661 caacgttcgt gatgggtgtg ggctcgcccg cctacatgtc acccgagcag gtcaagaact
     3721 atccgctgaa tcacaagacc gacctctact cgctgggcgt actgctgttc cagttgttga
     3781 ccggacggct gccgtttcga gccaacaacg ccgccgctct ggtttacaag atcgtcaaca
     3841 tggacgcgcc ttcggtctgc gcgttgcggc cgagtttgcc acccggcctc gacccgatca
     3901 tccggaaagc gctggagaaa gatctctaca gccgctaccg caatggcgcc gagttcgcca
     3961 aggacctgac gcccgtgcgc taccagatgc aggaagagga tcagacgcag caggatctga
     4021 cacacttcga gcgcttgcgg aacctgacgt tcttcgccga gttcgaggac atcgagttgt
     4081 gggaggtgct gcgcatcagc gtctggcggg aactgtcgga gaaagtgacc gtggtccgcg
     4141 aaggagataa tcaccgcaag tttggcgtga ttgttgacgg ctttgtcgag atatcttcca
     4201 atgggcgggt gatttgccga ctcggcccca gcgaggtggt cggagaaatg gcgttcctgc
     4261 atccgaccga tagccagcgt cacgccacag tggtcactct cgaacccacc ttgttccttg
     4321 aaatcaatag cgcggccctc gagttgagct ctgacgaggt acgggagcgc ttcaacaagg
     4381 tcttgctggt caaggtgctg ggccgcttgc gtgaggccaa caaggtcttg gcaaaactgg
     4441 gccagaccgc ggttcagggc agctttgcga gatcatcgct tggattgacg ccgcccctgg
     4501 agttgctgtg attccggttc atggggaaca aggactgcgc atgtcgacgg aaagcccggt
     4561 cacttcggtt agtggattcg agaaacgccg tcaagatgtg agacaatcac cggctgatga
     4621 tgccaaacgt gctccgcgct gcccggcagc tggtggcaat cgccgccgtc cgagccacgc
     4681 gttggcaacg ccttcctgca cgcatggaca cgccatatgc ctgatctcgc ttccccgacc
     4741 ttctggctcg ccgtactcca gatcatcgcc
//
LOCUS       DAOSIT010000126         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15914_length_4765_cov_9.057537, whole genome shotgun sequence.
ACCESSION   DAOSIT010000126 DAOSIT010000000
VERSION     DAOSIT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15914_length_4765_cov_9.057537"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLL14_04345"
     CDS             <1..405
                     /locus_tag="PLL14_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03184.1"
                     /translation="GTVVVLGMTGRNFAAGMSGGVAYVLDEEDSFESRCNMAQVSLEP
                     VEEELVARQGSDAGDDLEGHGKVDVRHLGVIDEVLLKGLVDKHYRFTGSRQALRLLND
                     WERCRRRFVKVMPHEYRRALNELAVQKQLEAA"
     gene            410..>1812
                     /locus_tag="PLL14_04350"
     CDS             410..>1812
                     /locus_tag="PLL14_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOG03185.1"
                     /translation="MGKPTGFIEYQRLAEASEPAASRLKHYREFVRVLTDEQASVQGA
                     RCMDCGIPFCNTGCPVNNIIPDWNDLVYRGNWEQALAVLHSTNNFPDFTGRICPAPCE
                     AACTLNINTDPVGIKSIEHAIVDKGWEKGWIVPQPPKLRTGRKVAIVGSGPAGLAAAQ
                     QLARVGHAVVVFEKNDRVGGLLRYGIPDFKFEKSHIDRRVEQMQAEGVEFRVNQEIGG
                     SGANAVPVGTLLAEFDALIVAGGAESPRDLTVPGRDLDGVHFAMEFLPQQNKVNAGDA
                     VPAQILATGKRVVVIGGGDTGSDCVGTSNRHGARSVTQFEVMPQPPEQENKSLTWPYW
                     PLKLRTSSSHEEGCTRDFAVSTKEFVGENGKLTALKVVRVNWSEGRMQEVPGSEVDIP
                     ADLVLLAMGFVAPVRQGLLQELAVDLDARGNVKATTDGAGCYATSVPKVFAAGDMRRG
                     QSLVVWAIREGRQCAREV"
     assembly_gap    1813..1912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1919..>3006
                     /gene="glmU"
                     /locus_tag="PLL14_04355"
     CDS             1919..>3006
                     /gene="glmU"
                     /locus_tag="PLL14_04355"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOG03186.1"
                     /translation="MNIVILAAGQGKRMHSNLPKVLHPLAGKALVQHVIETARSLAPQ
                     TLCLVHGHGGEAVRTRIDAQDLIWVLQDPQLGTGHAVMQALPHLKDAAITLVLYGDVP
                     LIQAETLQRLVHAARDALAILTVELADPGGYGRIVRNAGGDVLRIVEQKDASAEERAI
                     REVNTGIVALPTARLAEWLGRLSNDNAQQEYYLTDIVAMAVADGVPIRTAQPQGAWEM
                     LGVNSKVQLAELERIQQRRTAERLMEQGVRLADPARIDVRGELVCGRDVFIDVNCVFE
                     GRVVLEEAVEIGPACVLKNASIGAGSRLAAFSHIEDAIVGPDGVIGPFARLRPGTELA
                     AGVHVGNFVELKNSKFAAQSKANHLAYVG"
     assembly_gap    3007..3106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3107..>4765
                     /gene="glmS"
                     /locus_tag="PLL14_04360"
     CDS             <3107..>4765
                     /gene="glmS"
                     /locus_tag="PLL14_04360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOG03187.1"
                     /translation="RKLEYRGYDSAGLALIDESGLHRLRSVGRVAELTAQVDASQAAG
                     NTGIAHTRWATHGVPCERNAHPHISAGLAVVHNGIIENHESLRGRLRALGYEFTSDTD
                     TEVVAHLIAHELKNTPDFLTAVRQAIAQLLGAYAIAVLREADPSRLVVAREGAPLLLG
                     LSDDGCYAASDASALVQVTRRMVYLENGDCAELRRDSYHITRVDGIPVERAETQSQLS
                     ADAIELGQYRHYMQKEIFEQPVALANTLEMLGAARSIQPGVFGAESEVLLADVRQVLI
                     VACGTSYHAGLVSRYWLESIAGIPCSVEVASEYRYRDSVPDPQTLVVTISQSGETADT
                     LAALHHAKSLGMHHTLCICNVPESSLVRENALRFITRAGPEIGVASTKAFTTQLAALY
                     LLTLVLAKVRGRLDEQAEAAELHAVRHLPMAVAKILELEPQIREWAERFSMKQHALFL
                     GRGRHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVDREMPVIAVAPNDALLEK
                     LKSNLQEVRARGGELYVFADADSEMQESDGIHVLHLPEHYGALSA"
BASE COUNT          873 a         1321 c         1486 g          885 t
ORIGIN      
        1 gggacggtcg tcgtgctcgg gatgaccggt cgcaacttcg cggcgggcat gtccggtggc
       61 gttgcctacg tgctcgacga ggaagacagc ttcgagtcgc gctgcaacat ggcgcaggta
      121 tcgctggagc ctgtcgagga ggaactggtc gcccgtcagg gcagcgatgc gggtgacgat
      181 ctcgaaggcc acggcaaggt ggacgttcgc catctcggcg tgatcgacga agtgctgctc
      241 aaggggttgg tggacaaaca ctatcgtttt accggaagcc gccaggcgct gcgattgctc
      301 aatgactggg agcgctgtcg cagacgcttc gtgaaggtga tgccgcatga gtaccggcgt
      361 gccctcaacg aactggccgt gcaaaagcaa ttggaggccg cataggaaaa tgggtaagcc
      421 gactggattc atcgagtacc agcgtctggc agaggctagt gagcctgctg catcacgcct
      481 caaacattac cgcgagtttg tccgcgtcct gaccgacgaa caggccagtg tccagggcgc
      541 acgctgcatg gattgcggga tcccgttttg taataccggg tgcccggtaa acaacatcat
      601 tcccgactgg aacgaccttg tttaccgtgg aaactgggag caggcgctgg ccgtactgca
      661 ttcgaccaac aactttcccg attttaccgg gcgcatttgt ccggcaccgt gcgaagccgc
      721 gtgtacgctc aatatcaata ccgatcctgt aggcatcaag tcgatcgagc atgcgatcgt
      781 cgataaaggt tgggaaaaag gctggatcgt tccgcagcca ccgaagttga ggactggccg
      841 gaaagtcgcc atcgtcggct ccgggcctgc cggcttggcg gcagcgcagc aactggcgcg
      901 cgtcgggcat gcggtggtcg tctttgaaaa gaacgatcgc gtcggtggcc tgttgcgcta
      961 cggtattccg gatttcaaat tcgagaagtc gcacatcgac cgccgcgtcg agcagatgca
     1021 ggccgaggga gtcgagtttc gagtcaatca ggagattggc ggtagcggtg cgaacgctgt
     1081 tccggtcggc actctgctgg ccgaatttga tgcgctgatc gttgccggtg gtgccgaatc
     1141 gccgcgcgac ttgacggtac cgggcaggga tctcgatggc gtgcactttg ccatggagtt
     1201 cctgccgcaa cagaacaagg tcaatgccgg cgatgcggtg cctgcgcaga ttctggccac
     1261 cggcaagcgc gtggtggtca tcggcggtgg cgatacaggc tccgactgtg tcggtaccag
     1321 caatcgtcat ggtgcacggt cggttaccca gttcgaggtg atgccgcaac cacccgagca
     1381 agagaacaag agtctgacct ggccgtattg gccactgaag ttgcgcacct cgtcttcgca
     1441 cgaagaaggt tgcacacgcg actttgcagt ttccaccaag gaattcgtcg gtgaaaacgg
     1501 taaactgaca gccctcaagg tggtccgcgt gaactggagc gaaggccgca tgcaggaagt
     1561 tccgggcagt gaagtggaca ttccggccga tctcgtgctg ttggccatgg gttttgttgc
     1621 gccggtcagg caaggcctgc tgcaggaact ggcagtcgat ctcgacgccc gcggtaacgt
     1681 caaggcgacc accgatggtg cgggttgcta cgccaccagc gtgcccaagg tgtttgcggc
     1741 gggtgacatg cggcgagggc agtcgctcgt cgtctgggca attcgcgaag gacggcaatg
     1801 tgcgcgtgaa gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagctgat
     1921 gaacatcgtc attctcgctg ccggacaagg caagcgcatg cattcgaact tgccgaaagt
     1981 gctgcatcca ctggccggca aggcgctggt ccagcatgtg atcgagactg cgcgcagtct
     2041 cgctccacaa accctttgtc tggttcatgg ccacggcggt gaagcggtgc gtacgcgcat
     2101 cgatgcacag gatctgatct gggtgctgca ggatccgcaa ctcgggaccg gacacgcggt
     2161 catgcaggcc ctgccgcatc tgaaggatgc cgccatcacc ctggtgcttt atggagacgt
     2221 gccactgatc caggctgaga cgttgcagcg gctggtgcac gcagcgcggg acgcactcgc
     2281 catcctgact gtcgaacttg ccgacccagg cggttacggt cggatcgtgc gtaacgccgg
     2341 aggtgatgtg ctgcgcattg tcgagcagaa ggatgcctcg gccgaggagc gagccatcag
     2401 ggaagtgaat accggtatcg tggcgctgcc gacggcgcgc ctggccgaat ggctggggcg
     2461 cttgtcaaac gacaacgcgc agcaggagta ctacctgacc gacatcgttg ccatggcggt
     2521 ggccgacggg gtaccgattc gcacggcaca gccgcagggt gcgtgggaaa tgctgggggt
     2581 caatagcaag gtgcaactcg ctgaactcga gcgtatccag cagcgccgga cggcggagcg
     2641 cctgatggag cagggcgtcc gcctggccga tccggcgagg atcgacgtgc ggggtgaact
     2701 ggtttgtggc cgcgatgtgt ttatcgacgt gaactgcgtt ttcgagggca gggttgttct
     2761 cgaagaggcc gtcgaaatcg ggcctgcctg cgtgttgaaa aacgccagca ttggtgccgg
     2821 ttcgcgtctc gccgccttta gccatatcga agacgccatc gtcgggcctg acggcgtgat
     2881 cggccccttc gcgcgcttgc gtccgggcac ggaactcgct gccggcgtgc atgtgggcaa
     2941 ctttgtcgag ctgaagaaca gcaagtttgc cgctcaatcg aaggccaatc atctggctta
     3001 tgtcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgca agctggaata
     3121 tcgtggctac gattcggccg ggcttgcctt gatcgacgaa agcggtttgc accgattgcg
     3181 ttcggtcggt cgggtggcgg agctgactgc ccaggttgac gcatcgcagg ccgccggtaa
     3241 cacgggcatc gcgcacacgc gctgggcgac gcatggtgtg ccctgtgagc gcaacgcgca
     3301 tccgcatatc tctgccggtc tggcggtggt gcacaacggg atcatcgaga accacgagtc
     3361 actgcgcgga cgcctgcgtg ctctcggcta cgaatttact tcggatacag acaccgaggt
     3421 cgtggcgcat ctgatcgccc atgagttgaa gaacactcct gattttctga cggctgttcg
     3481 tcaggccatt gctcaactgc tgggcgctta cgccattgcc gtgctgcgcg aagcagaccc
     3541 aagccgtctg gtggtcgcac gcgaaggtgc gcccctgctg ctcggactca gtgatgacgg
     3601 atgctacgcg gcttctgatg cttcggcgct ggtacaggtc acgcgccgga tggtctatct
     3661 ggaaaacggc gactgcgctg aactcaggcg ggacagctat cacatcaccc gtgtcgacgg
     3721 catcccggtg gagcgggcag agacccagtc acagctttca gccgatgcca tcgaactggg
     3781 ccagtaccgg cactacatgc agaaggagat tttcgagcag ccggttgcgc tggccaacac
     3841 cctggagatg ctgggtgcgg ctcgcagcat ccagcccggc gtcttcggtg ccgaaagcga
     3901 agtgctgctt gcagatgtcc gacaggtgct gatcgttgcc tgtggaacca gctatcacgc
     3961 cggtctggtc tcccgttact ggcttgaatc catcgccggc atcccgtgct cggtcgaagt
     4021 ggccagcgag tatcgctatc gtgattcagt tcccgatcca cagactctgg tggtgacgat
     4081 ttcgcagtcg ggcgaaaccg ccgacacact ggcggcactc caccacgcca agtccttggg
     4141 aatgcatcac acgctatgca tctgcaatgt cccggagtca tccctggtgc gtgagaacgc
     4201 cctgcgcttc attacccgtg cgggcccgga aatcggcgtt gcctcgacca aggccttcac
     4261 cacgcaactg gccgcactgt acctgctgac actggttctg gccaaggtcc gcggacgcct
     4321 ggacgaacag gccgaggccg cggaactgca tgcggtgcgg cacctgccga tggccgtggc
     4381 caagatcctc gaactggagc cgcagattcg cgaatgggcc gagcgcttct cgatgaagca
     4441 gcatgcactc ttcctcgggc gtggccgaca ctacccgatt gcgctggagg gcgcgctcaa
     4501 gctcaaggaa atctcctaca tccacgccga ggcctatccg gccggagagt tgaagcacgg
     4561 tcctctggct ctggtcgatc gggaaatgcc ggtgatcgca gtcgctccca acgacgcgct
     4621 gctcgaaaag ctcaagtcca acctgcagga agtgcgcgcc cgcgggggcg aactctacgt
     4681 ctttgccgat gcggatagcg aaatgcagga gtcggacggc attcatgtgc tccatctgcc
     4741 tgagcactac ggtgctctat cggct
//
LOCUS       DAOSIT010000127         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_15978_length_4749_cov_9.875160, whole genome shotgun sequence.
ACCESSION   DAOSIT010000127 DAOSIT010000000
VERSION     DAOSIT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15978_length_4749_cov_9.875160"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLL14_04365"
     CDS             <1..600
                     /locus_tag="PLL14_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG03188.1"
                     /translation="LEVLLSRREREPFAEQWALPGGYVHAVEDADSLAAARRVLREKA
                     CLTSPYLEQLYTFSGANRDPRGWSASIAYYALVSADTLRPASPAAFERWPVDELPRLC
                     FDHARIVDCALERLRSKSAYSSLPCYLLPPTFTLTELQQTYEHVLGQKLDKSAFRRKL
                     ADFDFLEVVKDAQRTGSHRPAQLYRIREEKNLMLFDRTI"
     gene            complement(629..>1186)
                     /locus_tag="PLL14_04370"
     CDS             complement(629..>1186)
                     /locus_tag="PLL14_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG03189.1"
                     /translation="QSSLRAAIGIVPQDTVLFNDTIYYNILYGQPEASREQVLAAAES
                     AQLAGFIERLPDGYETRVGERGLKLSGGEKQRVAIARALLKNPPFLIFDEATSALDSK
                     TEKAIQASLDSAARGRTTLIIAHRLSTIMNADEILVMNAGRIIERGSHPQLLDAGGSY
                     AQMWALQQLEDSGNALADEQAGAIQ"
     assembly_gap    1187..1286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1287..2326)
                     /locus_tag="PLL14_04375"
     CDS             complement(<1287..2326)
                     /locus_tag="PLL14_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOG03190.1"
                     /translation="MRRAASPSLPTDRPPTADSPLWPTLHKLLPYLWQYKWRVFLALS
                     CLFSAKLANVAVPLVFKEMIDGLSGTPQTLALPALLLLLYGALRFSTALFTELREILF
                     ARVTQRAVRRIALEVFRHLHALSLRFHLQRQTGGVSRDIERGSRSISSLISYTLYSIL
                     PTLVEISLVLGILFARYDSSFVLITLASLSAYVVFTIKVSNWRIAIRRKVNDTDSAAN
                     TRAIDSLLNYETVKYFNNEEFEARRYDQQMQHWEDAATRSQVSLSWLNLGQQIIIALG
                     VTAMMWRAADGVVAGTMTIGDLVLVNAFLIQLYMPLNFLGVVYREIRQSLADIERMFD
                     LLAVNREIADAV"
     gene            2524..3282
                     /locus_tag="PLL14_04380"
     CDS             2524..3282
                     /locus_tag="PLL14_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG03191.1"
                     /translation="MTDQKTTLDTRERILDVAERLFMENGYEGTSMRVITGAAEVNLA
                     AVNYHFGSKEALLREVFRRRLAWLNHQRLEALDRLEEHAAGAPLKPSQILEAFFGTLL
                     RMGEDPALGGMTFLRLLGRTLTEPAEFIRTFFAGEYAQVIDRYKLALFRALPDVPKAE
                     IVWRLHFMLGAMSYAIAGTDVLQVVTGCEVGDLAGEGADADPSDTLLARRLAQRLMPF
                     LLGGLRAPLPQFHELPVTTTDTRARAVPQDGLRP"
     gene            3326..>4749
                     /locus_tag="PLL14_04385"
     CDS             3326..>4749
                     /locus_tag="PLL14_04385"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOG03192.1"
                     /translation="MITTIISTLIGITLLSLAVLFGVRPLRRALVSRPIFRTYKRILP
                     QMSETERVALEAGTVWWDGELFRGNPDWNKLLAYPVPKLTAEEQAFMDNEVEQACALV
                     DDWQVTTELHDLSPQAWQYIKEHGFLGMIIPKKYGGLEFSAYAHSQIVTKLSTRCSAL
                     SVSVMVPNSLGPAELLLHYGTDAQKNHYLPRLAKGIEIPAFALTSPWAGSDAGSIPDV
                     GIVCKGMWQGKEVLGMRVTWDKRYITLGPVCTILGLAFHLYDPEGLLGGRKHVGITCA
                     LVPRDTPGAEIGRRHFPLNAMFMNGPTRGKDVFMPLDYVIGGPAMVGQGWRMLMECLA
                     AGRSISLPSSNTGMAQLTARTVGAYARVRSQFKMAIGRFEGVEEPLTRIGAYTYMMDA
                     VRKMTAGAIDLGEKPSVVSAIAKYHVTERARQVVNDGMDIVGGKGICLGPSNFIGRAY
                     QQLPIGITVEGANILTRSMILFGQG"
BASE COUNT          842 a         1396 c         1522 g          889 t
ORIGIN      
        1 ctggaagtgt tgctgtcacg gcgggagcgc gagccctttg ccgaacagtg ggccttgccc
       61 ggcggctatg tgcacgccgt cgaggatgcc gatagcctgg ctgcggctcg ccgggtcctt
      121 cgggaaaagg cctgcctgac ctcgccatac cttgagcagt tatatacctt ctccggtgcc
      181 aaccgagacc cgcgcggctg gtcggcgagc atcgcctact acgccctggt cagcgcggac
      241 acgcttcgac cggctagtcc ggcggcgttc gagcggtggc ctgttgatga gttgccgcgg
      301 ctgtgtttcg accacgcgcg tatcgtcgac tgcgcgctcg aacgtctgcg cagcaagtcg
      361 gcctactcat cgctgccgtg ttacctgttg ccgcccacgt tcactctgac cgagttgcag
      421 cagacctacg aacacgtgct ggggcaaaag ctcgacaaga gcgctttccg ccgcaagctc
      481 gccgacttcg attttctcga agtggtcaag gacgcgcagc gtaccggcag ccatcgtccg
      541 gcccagctct accgtattcg cgaagagaag aacctgatgc tcttcgaccg gacgatctag
      601 gcttccagcg caaccctccc agcaggagct attgaattgc gccggcctgt tcgtcggcca
      661 gggcattgcc ggagtcttcc agttgctgca gcgcccacat ttgcgcgtag ctgccgccgg
      721 catcgagtag ctgcgggtgg gagccgcgct cgatgatgcg gccggcgttc atgaccagaa
      781 tctcgtcggc attcatgatc gttgacaaac ggtgcgcgat gatcagtgtc gtgcggccgc
      841 gcgccgcgct gtcgaggctg gcctggatgg ccttttcggt cttcgagtcg agagccgaag
      901 tcgcttcgtc aaagatcagg aacggcggat ttttcagcag ggcacgggcg atggcgacgc
      961 gttgtttctc gcctcccgag agcttgaggc cgcgttcgcc gacgcgggtt tcgtagccgt
     1021 cgggaagccg ctcgatgaat ccggccagtt gcgcactttc ggcggcggcc agcacctgtt
     1081 cgcggctggc ttccggctgg ccgtagagga tgttgtagta gatcgtgtcg ttgaacagta
     1141 ccgtatcctg gggaacgatg ccgatcgccg cgcgcaggct gctttgnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnntcca ccgcgtcggc gatttcccga ttgaccgcca
     1321 gcaggtcgaa catgcgttcg atgtccgcca gggactggcg aatctcgcga tagacgacgc
     1381 cgaggaaatt gagcggcatg tacaactgaa tcaggaaggc attgaccagc accagatcgc
     1441 cgatggtcat ggtgccggcg acgacgccat ccgcggctcg ccacatcatc gccgtgacgc
     1501 ccagcgcaat gatgatctgc tggccgagat tgagccacga gagcgagacc tggctgcggg
     1561 tggccgcgtc ctcccagtgc tgcatctgct ggtcgtagcg cctggcttcg aactcctcgt
     1621 tgttgaaata cttgactgtt tcgtagttca gcaggctgtc gatggcgcgg gtattggcgg
     1681 cggaatcggt gtcattgacc ttgcgccgga tggcgattcg ccagttgctg accttgatcg
     1741 tgaacaccac gtaagcgctc agcgacgcca gggtgatgag cacgaaggaa ctgtcgtagc
     1801 gggcaaagag gatgcccagt accaggctga tctcgaccag ggtcggcagg atcgaataca
     1861 gggtgtagct gatcaggctt gagatcgagc ggctgccgcg ttcgatgtcg cgagagacgc
     1921 cgccggtctg gcgttgcagg tgaaagcgga gcgagagcgc gtgcaggtgg cgaaatacct
     1981 cgagcgcaat gcgccgtacc gcgcgctggg tgacgcgggc gaagaggatt tcgcgcagtt
     2041 cggtaaacaa ggccgtcgaa aagcgcagcg ccccgtagag caaaagcagc aaggcgggca
     2101 gggcgagcgt ctgcggcgtg ccgctcaggc cgtcgatcat ttccttgaac accagcggca
     2161 cggccacgtt ggccagtttg gcgctgaaca gacagctcag ggccaggaat actcgccact
     2221 tgtattgcca caggtacggc agcagcttgt gcagggtagg ccagagaggg ctgtcggccg
     2281 tcggcggtcg atcggtggga agggaagggg aggcggcgcg gcgcatgggg ctggcttgtc
     2341 gatgaggtag ctgagccccg gagtatgcct tgttggcgag tcgaggctca atagcgatgc
     2401 cgaaagacgc aaaaaagcct tgcattacag gggttctggc cgtaccatta aaacgaacgt
     2461 ttgaacgaac cgcggtggcg atctgtcgcc gtacgctgcg gctttccctg ggcccgtgat
     2521 ctcatgaccg atcagaaaac cactctcgat acccgcgaac gcattctcga cgtcgccgaa
     2581 cgcctgttca tggaaaacgg ttacgaaggg acttcgatgc gggtgatcac cggcgccgcg
     2641 gaagtcaacc tggcggcggt caattaccat ttcgggtcga aggaggcgct gctgcgcgag
     2701 gtgttccggc ggcgactggc ctggctcaat caccagcgtc tggaggctct cgaccgactc
     2761 gaggaacacg ccgccggcgc gccgctgaag ccgtcgcaga ttctcgaagc ctttttcgga
     2821 accctgttgc gcatgggcga agacccggcg ctgggcggca tgaccttcct gcgcctgctc
     2881 ggccgcaccc tgaccgagcc ggccgagttc atccgcacgt tcttcgccgg tgagtatgcg
     2941 caagtcatcg atcgctacaa gctcgcgctg ttccgggcct tgccggatgt tccgaaagcg
     3001 gagattgtct ggcgcctgca tttcatgctc ggtgccatgt cctacgcgat tgccggcacc
     3061 gatgtgctgc aggtggtcac cggctgtgag gtgggcgatc tggccggcga aggggcggac
     3121 gccgatccca gcgacacgct gctggcccga cgcctcgcgc agcggctgat gcctttcctg
     3181 ctcggcggct tgcgggcgcc gttgccgcag ttccatgaat tgcccgtaac cacaaccgac
     3241 acaagagcaa gagccgttcc ccaagacggc ctcagacctt agaactgagc cctgaaaccg
     3301 aagaaccatg ataggagaag cagcaatgat caccacgatc atctcgacac tcatcggcat
     3361 cacgctgttg agcctggccg tgctgttcgg cgttcgtccc ttgcgacggg cgctggtcag
     3421 ccggccgatt tttcgtacct acaagcggat cctgccgcag atgtcggaaa ccgagcgggt
     3481 ggccctcgag gccggcacgg tgtggtggga cggtgaactg ttccgcggca accccgactg
     3541 gaacaagctg ctcgcctatc cggtgccgaa gctgaccgcc gaagaacagg cattcatgga
     3601 caacgaggtc gagcaggcct gtgcgctggt cgatgactgg caggtgacca ccgagttgca
     3661 cgatctttcg ccacaggcct ggcaatacat caaggagcac ggcttcctgg gaatgatcat
     3721 cccgaagaaa tacggcggtc tcgagttctc ggcctacgcg cactcgcaga tcgtcaccaa
     3781 gttgtctacg cgttgctcgg cgttgtcggt gtcggtgatg gtgcccaatt cgctgggtcc
     3841 ggccgaactg ctgttgcact atggtactga cgcgcagaag aaccactacc tgccacgtct
     3901 ggcgaagggt atcgagattc cggcgtttgc gctgaccagt ccgtgggccg gttccgacgc
     3961 cggttcgatt cccgacgtcg gcatcgtctg caagggcatg tggcagggca aggaagtgct
     4021 gggcatgcgt gtgacctggg acaagcgtta cattacgctg ggaccggtgt gcaccatcct
     4081 cggactggct ttccatttgt atgacccgga ggggctgctc ggtggcagga agcacgtcgg
     4141 gatcacctgc gcgctggtac cgcgcgacac ccccggtgcg gaaatcggcc gtcggcactt
     4201 cccgctcaac gcgatgttca tgaacggccc gacgcgcggc aaggacgtgt tcatgcccct
     4261 cgattacgtg atcggtgggc cggcgatggt cggccagggt tggcggatgc tgatggagtg
     4321 tctggcggct ggccgctcga tctcgctgcc gtcgtcgaat accggcatgg cccaactgac
     4381 cgcgcgcacc gtcggcgcct acgcgcgcgt ccgtagccag ttcaagatgg cgatcgggcg
     4441 cttcgaaggc gttgaagagc cgctgacccg tatcggagcc tacacctaca tgatggacgc
     4501 cgtgcgcaag atgaccgccg gcgccatcga cctcggcgag aagccgtcgg tggtttcggc
     4561 gatagccaag taccatgtca ccgagcgcgc ccgtcaggtg gtcaatgacg gcatggatat
     4621 cgtcggtggc aaggggatct gcctcggccc ctccaacttc attggccggg cctatcagca
     4681 gttgccgatc ggaatcaccg tcgaaggcgc gaacatcctc acgcgcagca tgatcctgtt
     4741 tggtcaggg
//
LOCUS       DAOSIT010000128         4742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16007_length_4742_cov_9.377000, whole genome shotgun sequence.
ACCESSION   DAOSIT010000128 DAOSIT010000000
VERSION     DAOSIT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16007_length_4742_cov_9.377000"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1062)
                     /locus_tag="PLL14_04390"
     CDS             complement(175..1062)
                     /locus_tag="PLL14_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG03193.1"
                     /translation="MVISDPAVRARPVGFVCIVASAAGFGVMPIFAKLAYAEGVDLPS
                     MLFLRFALAGLLMAVLMVLQGLRWPRGRNLLLLVAMGGAGYVGQSFCYFAALQHATAG
                     LTALLLYLYPAIVTVLSALLARRRLSPLRLLAVAAALFGTGLAVGGSLAGSVLGIILG
                     VAAALIYSVYILVGERVTRVSGAMPSATVIMLAAAAVYGLIVFSRGPAFPVSLAAWTA
                     VGGIALFSTVVAMITFFAGMARLGAADAATLSTLEPVVTVLLAALFLGEEVGGWQLAG
                     GAIILTAVIVLARSGEAGG"
     gene            1346..2512
                     /locus_tag="PLL14_04395"
                     /pseudo
     CDS             1346..2512
                     /locus_tag="PLL14_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
     gene            complement(2691..4607)
                     /locus_tag="PLL14_04400"
     CDS             complement(2691..4607)
                     /locus_tag="PLL14_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG03194.1"
                     /translation="MPYLILSDASLAFGHVPLLDHADFQLDPGERVALIGRNGTGKSS
                     LLSALAGWGGLDDGKVWQQPGLRIGYVPQEPPFDPDLTVFAAVVAGMGELSALLAEYH
                     AVSHALGEPEADHSRLLERMQVLQTELESRHAWSFEAQAERVIQRFSLDADTLVGTLS
                     GGQKKRLALAQALAISPDLLLLDEPTNHLDVDAIEWLEEMLVASGVSLVFITHDRRFL
                     ERVATRIVELDRGRLLSCPGRFSEYQARKEAMLHDEALNNARSDKFLAQEEVWIRQGI
                     KARRTRNEGRVLRLERLRRERAARRERQGKVELALDAGDRSGKLVAALDRVGKCFGDK
                     VVVRDFSCRVQRGDKIGIIGPNGAGKTTLLRLILGELAPDQGKVRLGTKVQVAYFDQF
                     RSQLDEQATLIDCISPGSDFVEIGSQRKHVISYLGDFLFAPQRARSLVSSLSGGERNR
                     LLLARLFARPANVLVLDEPTNDLDIETLELLEELLQDYSGTLFLVSHDRAFIDNVVTQ
                     TIAAEGDGGWREYAGGYQDWADYQATRRREEALATGASKRTEPRPAELSRTTRQKTVV
                     VSKRSWKETHELAELPQRIAALEDEQKVIGERLADPTLYHSQPQEAQRLAARLSEIDD
                     QLLSLLVRWEALES"
BASE COUNT          849 a         1626 c         1449 g          818 t
ORIGIN      
        1 tcacgtcatc aaccaccgac cgaacctgcc gcgcaatccc ttcctgcccg agcacacttc
       61 gggtaaccag gtcattcttg gcgaagcgct ggctgccgtc ctcccggatg tactgcatcg
      121 tccattgcct gccgtcgacg tcggcggcct gggccagcca gcccactacg ccagctaccc
      181 gccagcctcg cccgagcgtg ccagaacgat caccgcagtg aggatgatcg ccccgccggc
      241 gagctgccag ccaccaacct cctccccgag aaacagcgcg gccaggagca cggtcaccac
      301 cggttcgagc gtcgacagcg tcgccgcatc ggcagcaccc agccgcgcca tgccggcaaa
      361 aaaggtgatc atcgccacca ccgtcgaaaa cagcgcaata ccgccgaccg ccgtccaggc
      421 tgcgagcgag accggaaacg cgggcccgcg cgagaagacg atcaaaccgt atacggccgc
      481 cgccgcaagc atgatcaccg tcgctgaagg cattgccccg ctgacccgcg tgacgcgctc
      541 accaaccagg atgtagaccg agtagatcaa cgccgcagcc accccgagga taatgcccag
      601 gacgctgccc gcgaggctgc caccgaccgc caggccggtt ccgaacaagg ccgcagccac
      661 cgccagcagg cgcagcggcg acaagcgtcg gcgggccagc agcgccgaga ggacggtgac
      721 gatcgccggg tacagataca gcaacagtgc ggtcagtccg gcggtcgcat gctgcagggc
      781 agcaaaatag cagaacgact gcccgacata acccgctccc cccatcgcca ccaacaggag
      841 cagattgcgc ccgcgcggcc agcgcagccc ctgcagcacc atcagcacag ccatcaacag
      901 cccggccagc gcgaaacgca ggaaaagcat gctcggcagg tcgacgccct cggcgtaggc
      961 cagcttggcg aagatcggca tgaccccgaa acccgcagcc gaagcaacga tgcagacgaa
     1021 gccgacagga cgcgcacgta cagcaggatc agagatcacc atgtcaacct ttgtgaggtg
     1081 tcaccgcggt ggcgatgcct gcgcctgccg cctgacctcg ggagttggcc gagaccaagg
     1141 cggttggagc cacggttccg gacggtcgcc acagccgacc gtcggccggc acttgtcggg
     1201 tctggtcaat ggatcgaatt tccgggatta tacggcgctt gcagcaaccc gttgcgtcga
     1261 actcccgaag cgcagcggcg gccgggccat cgatcgatgt ctgccggccg gcactcacca
     1321 ccttttcaga ccggcagagg acgccatgcg gctatttgac gatttttcca tggcgtccct
     1381 gatggccggt ttcgtcgccg tcctggttgg cttcaccagt tcggcggtga tcgttctcca
     1441 ggccgcccgg acgctgcacg cgacaccggc cgaaatcgct tcgtggatgg gtgcccttgg
     1501 cctgggcatg ggtctgacca gtattgccct ctcgctgcgc ttccgggtac cgattctcac
     1561 cgcctggtcg acgcccggag cggcaatgct gatcaccagc gccgcaggcg tctcgatgcc
     1621 cgaggcgatc ggcgcctttc tggtttccgc gacgctgatc accgtctccg gattcagcgg
     1681 ctgcttcgaa aggatgatcg gtcgcatccc gatatcgatt gcctccggca tgctcgccgg
     1741 ggtcttgctg cgatttggtc tcgatgcctt cgtcgccatg caacagcggt tcacgctggt
     1801 atttccgatg ttctgcgcct atctgctcgg tcgccggctt tcgccacgct acgccatcgt
     1861 cgtcacgctg ctgctcggcg tgggcattgc cgcgacacag gggctgttgc acgtcgaagg
     1921 gctgcagctc gaactggcga cgccggtatt caccgttccg gaactgtcgt ggcgggcgct
     1981 ggtcggcgtc gcgctgccgc tctatgtggt aaccatggcc tcgcagaacg tgccgggcgt
     2041 ggccgtcatc cgcgcctcgg gctaccaggt gccgatttcg ccgctgatcg gctggaccgg
     2101 cgcggcgaac ttcctgcttg cgcctttcgg cggcttcgcc ttgaacctgg cagcgattac
     2161 cgcggccatc tgcatgagcc gcgaagccca tcaggacccg gagaaacgtt acaccgccgc
     2221 gatcgccgcc ggtttcttct atctgttgat cgggctcttc ggggccaccg tcggcgctct
     2281 tttcctggcc ttcccgaacg aactggtggt gaccatcgcc ggcttcgccc tgctcggcac
     2341 catcggcagc gctctgacca ccgccctggg ccaggaaagc gcacgcgaac ccgcgctgct
     2401 gaccttcctg gtcaccgcct cgggcattgc cctggcggga atcggctcgg ctttctgggg
     2461 cctgctggcc ggtcttgcgg ccctgctcgt gctgcaggcc aggcccgcgc cactgcgctg
     2521 gccggcgagt cgccgcgcag ccggtgcagc aggacaggcg ccgacgccag acgaccagca
     2581 cggcgaagag caatcccgct gaaccagtgc ctgccgtcca gcggaaattc gcgcacgcct
     2641 ccgaccgcat ggcgacgagg aaagccggca gttttgccgg caaccgccga ctagctttcg
     2701 agcgcttccc agcgcacgag cagcgacagc agttgatcgt cgatttcgct cagacgcgcc
     2761 gccagccgtt gcgcctcctg cggctgcgag tgatagagcg tcggatcagc caggcgctcg
     2821 ccgatcacct tctgttcgtc ctccagggcg gcaatgcgtt gcggcagttc ggccagttcg
     2881 tgcgtctcct tccatgaccg cttgctgacc acgaccgttt tctgccgggt ggttcttgag
     2941 agctctgccg ggcgtggttc agtgcgtttg ctcgcgcccg tggcgagcgc ctcttcacgc
     3001 cgccgagtgg cctgataatc ggcccaatcc tgataaccgc cggcgtattc ccgccacccg
     3061 ccgtcgcctt cggctgcgat cgtctgggtg acgacgttgt cgatgaatgc ccggtcgtgg
     3121 ctcaccagaa acagcgtacc gctgtagtcc tgcaagagtt cctcgagcaa ttccagggtc
     3181 tcgatgtcga ggtcattggt cggttcgtca agcaccagca cattcgccgg gcgcgcgaac
     3241 aggcgcgcca gcagcaaacg attgcgctcg ccgccggaca gtgaactgac cagtgaacgc
     3301 gcacgctgcg gggcaaacag aaaatcgccg agatagctga tgacatgctt acgctggctg
     3361 ccgatctcga caaaatccga tccgggagaa atgcagtcga tcagcgtcgc ctgctcgtcg
     3421 agctggctcc ggaactgatc aaaataagcc acctgcactt tcgttcccag gcgcaccttg
     3481 ccctgatcag gcgccagctc accgaggatc aggcgcagca gtgtcgtctt gcccgcaccg
     3541 ttcgggccga tgatgccgat cttgtcgcca cgctgcacgc gacaggaaaa atcacgcacc
     3601 actaccttgt cgccgaagca cttgccaacc cgatcgagcg ccgccaccag cttgccgctg
     3661 cgatcgccgg catccagcgc gagttcgacc ttgccctgac gctcgcgccg cgcagcccgt
     3721 tcccgacgca ggcgctccag gcgcagcacg cggccttcgt tgcgggtgcg acgagccttg
     3781 atgccctgcc tgatccacac ctcttcctgg gccagaaact tgtccgatcg cgcgttgttc
     3841 agcgcctcgt catgcagcat ggcttccttg cgcgcctgat attcgctgaa gcggccagga
     3901 cacgaaagca gtcgtccccg gtcgagctcg acgatgcgcg tggcgacgcg ttcgagaaag
     3961 cggcgatcat gggtgatgaa caccagggaa acgcccgaag caaccagcat ctcctccagc
     4021 cattcgatgg cgtcaacgtc cagatggttg gtcggctcat cgagcagcaa caggtcgggt
     4081 gagatggcca acgcctgcgc caacgccagg cgctttttct gtccgccgga cagcgttccc
     4141 accagggtat cggcgtcaag cgagaagcgc tggatgacgc gctcggcctg tgcttcgaac
     4201 gaccacgcat gccgcgattc gagttccgtc tgcaacacct gcatgcgctc aagcagtcgg
     4261 ctgtggtccg cctccggttc accgagcgcg tgcgatacgg catggtactc ggcaagtagc
     4321 gcagacaact cgcccatgcc ggcaaccacc gcggcaaaga ccgtcagatc cgggtcgaaa
     4381 ggcggttcct gagggacata gccgatacgc agccccggtt gttgccagac cttgccgtcg
     4441 tccagccctc cccaaccggc gagggcgctc agcagcgagg acttgccggt accgttgcgg
     4501 ccaataagcg ccacccgttc tccagggtcg agctggaagt cggcatggtc gaggagcggc
     4561 acatggccga aagcaaggga ggcatcggag agaatcaggt agggcatggg tgcttgtcaa
     4621 tttcctggag ttgctgaaga tcgtggtcgg gcagaggcag ggcaagacgg gttcggcggt
     4681 caacgtcgcg cagtttagca gccttacatg gtcgcccctg ggtttgccaa gcattcaata
     4741 ta
//
LOCUS       DAOSIT010000129         4729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16052_length_4729_cov_7.214377, whole genome shotgun sequence.
ACCESSION   DAOSIT010000129 DAOSIT010000000
VERSION     DAOSIT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16052_length_4729_cov_7.214377"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLL14_04405"
     CDS             <1..270
                     /locus_tag="PLL14_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03195.1"
                     /translation="NAEIVGDQIQVHVDDDGIGIPTGERERVFSAFTRLDRSRDRSTG
                     GYGLGLAIARLVLEQHSGTAFAGESPLGGARFTLSWPLSQNVTES"
     gene            349..504
                     /locus_tag="PLL14_04410"
     CDS             349..504
                     /locus_tag="PLL14_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03196.1"
                     /translation="MSEFNLNYERFLAATRQHFFAGDLGSASAQDTEDDSLDACGETA
                     HRPHANT"
     gene            537..698
                     /locus_tag="PLL14_04415"
     CDS             537..698
                     /locus_tag="PLL14_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03197.1"
                     /translation="MAEGIGIFVGIVAWDVLSVGEMELLKASLVATAGALVWYGVRCW
                     LTSNRRKGP"
     gene            944..>1479
                     /locus_tag="PLL14_04420"
     CDS             944..>1479
                     /locus_tag="PLL14_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG03198.1"
                     /translation="MKYQIIPVTPFAQNCSLLWCEQTQRGAVVDPGGDVSRIMRAVEA
                     NGVRLEKILVTHGHIDHAGAVAEMASRLSLPIEGPQREDKFWIDGMPAQSKMFGFPNV
                     LAFTPDRWLEQGDTVCFGNVEMDVLHCPGHTPGHVVFFNRCGRLAIVGDVLFQGSIGR
                     TDFPKGDFDTLISSIRDRL"
     assembly_gap    1480..1579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1580..2889)
                     /gene="lpdA"
                     /locus_tag="PLL14_04425"
     CDS             complement(<1580..2889)
                     /gene="lpdA"
                     /locus_tag="PLL14_04425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG03199.1"
                     /translation="MSKQFDVLVVGAGPGGYIAAIRAAQLGFNVACCESNAYADPKGE
                     PRLGGTCLNVGCIPSKALLHTSHVFEEAGHGFAEQGISVGTPTIDVAKMVGRKDGIVK
                     QLTSGIKGLFKKNKVTLLNGHGAFVGRNGDAGSEVWQLRVGDELVEAKQVIVATGSKA
                     RHLPGVPVDNEIVCDNVGALDIDIVPKKLAVIGAGVIGLEMGSVWRRLGADVTLLEAL
                     PDFLSVVDVDVAKEAAKVFAKQGLKIVTGIEIGDVKISQKGVSIDYTDKGGNEQKLDA
                     DRLIVSIGRIPNTDGLDADKVGLHINERGQIVVDAHCRTNLPGVWALGDVVAGPMLAH
                     KAMEEGVMVAEIMAGQAGHCNFDTIPWVIYTSPEIAWVGRTEQQLKAEGVAFKTGKIP
                     FAANGRALGMGEPIGFVKMLACADTDRILGVHIIGANASELISEA"
     gene            complement(2982..4235)
                     /gene="odhB"
                     /locus_tag="PLL14_04430"
     CDS             complement(2982..4235)
                     /gene="odhB"
                     /locus_tag="PLL14_04430"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOG03200.1"
                     /translation="MIIDVKVPQLSESVAEATLVSWHRKAGEAVARDDNLIDIETDKV
                     VLELPAPDSGVLVEIVKSDGETVVAGEVIARIDTAAQAGTVAAAAAKPVATTPEATVA
                     PTAAAVATASGVALPAARKILEEKGVAASDVAGTGRGGRVTKADALAAQIPAVKPAAA
                     AAPAPAASTAAVLAPAPTVTVPLGDRPEQRVPMSRLRARVAERLLQSQASNAILTTFN
                     EVNMGPIMALRKQYGDRFEKAHGVRLGFMGFFVKAAVAALQKFPVINASVDGNDIVYH
                     GYIDIGIAVGSPRGLVVPILRDADQMTIADIEKKIGEFGVKAKDGKLSMEDLTGGTFS
                     ISNGGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIRPINYLAMSYDHRIID
                     GREAVLGLVTMKEALEDPARLLLGV"
     gene            complement(4248..>4729)
                     /gene="sucA"
                     /locus_tag="PLL14_04435"
     CDS             complement(4248..>4729)
                     /gene="sucA"
                     /locus_tag="PLL14_04435"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766863.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOG03201.1"
                     /translation="RHKDATSTLEELSEGQFRPVIGEVEPINAKKVTRVVFCSGKVYY
                     ELVAARHERKISHVAIARLEQFYPFPQESFQEELAKYPKATEVVWCQEEPRNQGAWYW
                     IASRQHLSRSLSDKQHLLLVSRPASASPAVGYHSKHNAQQKALIESAFGEIEYYKLP"
BASE COUNT          906 a         1460 c         1338 g          925 t
ORIGIN      
        1 aacgcggaaa tcgtcggtga ccagatccag gtgcatgtcg acgacgacgg tattggcatc
       61 cctaccgggg aacgcgagcg ggtgttctct gccttcaccc gcctcgaccg ttcgcgtgac
      121 cgatcgaccg gcggctatgg gcttggtctg gcgatcgcgc gactggtact ggagcagcac
      181 agcggcactg cctttgccgg agaatcaccg ctgggcggcg cccgcttcac gctgagttgg
      241 ccgctgtcac aaaacgtcac tgaaagctga cgcaaacata gaagactctc tggccggtcg
      301 gcccctatca tcacacctgt tcacaaaaca atgatagagg aagacgacat gagcgaattc
      361 aatttgaact acgagcggtt tcttgccgcc acccgccaac atttcttcgc cggcgacctg
      421 ggttcagcct ctgcacagga taccgaggat gattcattgg acgcctgcgg cgaaacagca
      481 catcgcccac acgccaatac atgagcgcag tggcgcaacc ccctgtgccc ttcccgttgg
      541 ctgaaggaat cggtatcttt gtcgggattg tcgcctggga cgttttgagc gtcggagaga
      601 tggagctcct caaggcttcg ctggttgcca ccgccggcgc gctggtctgg tacggtgtac
      661 gctgctggct aacctccaac cgccgcaagg ggccctgaac aagaatagct ggttcctgac
      721 cggtttaccc ttccctcacg cccgttgcca tgacgggcct tgattcccgc gccactcccc
      781 cggcgcgggt ttttttttgc ccatgcccgg ccacccccag gaaggtccac gacgttgcat
      841 gcatgcgagt gacctggctg atcgccccac gcacgacgag tctgagcgac gctctgctat
      901 actcacccgc tctcttcacc caacaccagc gccagcgcag accatgaaat atcagatcat
      961 cccagtcact cctttcgcac aaaactgcag ccttctgtgg tgcgagcaaa cgcaacgagg
     1021 cgcggttgtc gatccgggag gcgacgtgtc gcggatcatg cgtgctgtcg aggccaacgg
     1081 agtcaggctg gagaagatcc tggtcacgca cgggcatatc gatcacgccg gtgcggtagc
     1141 cgaaatggcg tcgcgcctct ccctgcctat cgaggggccg cagcgcgagg ataagttctg
     1201 gattgatggc atgccggcac agagcaagat gttcggcttc ccgaacgtgc tggcctttac
     1261 tccggaccgc tggcttgagc agggcgacac ggtatgtttc ggcaacgtcg agatggatgt
     1321 cctgcattgt cccggacaca cgccgggaca cgtcgtcttc ttcaaccgct gcggccgact
     1381 ggcgatcgtc ggcgacgtgc tgttccaggg gtcgatcggt cgcaccgatt tccccaaagg
     1441 cgatttcgat acgctgatct cctcgatccg cgaccggctn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnng cttccgagat caactcggac gcgttcgcac cgatgatatg
     1621 gacgcccagg atgcggtcag tgtcggcaca ggcaagcatt ttgacaaaac cgatcggttc
     1681 ccccatgcca agcgcacgac cattcgcggc gaagggaatc ttgcctgtct tgaaggccac
     1741 gccctcggcc ttgagttgct gctcggtcct gccgacccag gcgatctccg gactggtgta
     1801 gatcacccat gggatggtgt cgaaattgca gtgccccgcc tgaccagcca tgatctcggc
     1861 aaccatgacc ccctcttcca tcgccttgtg cgccagcatc gggccggcaa cgacgtcacc
     1921 caacgcccaa acaccgggca gattggtccg gcaatgggca tcgaccacga tttgtccgcg
     1981 ctcgttgata tggaggccca ccttgtcggc atccaggccg tcggtgttcg ggatgcggcc
     2041 aatcgatacg atgaggcgat cggcgtcgag cttctgctcg ttgccgccct tgtccgtata
     2101 gtcgatcgaa acgcccttct gcgagatctt gacgtcgccg atttcaatcc cggtcacgat
     2161 tttgaggccc tgcttggcaa acactttggc cgcttccttg gcaacatcaa cgtcaaccac
     2221 ggagagaaag tccggcagtg cttcgagaag ggtcacgtcg gcaccgagcc ggcgccagac
     2281 gctacccatt tccaggccaa taacgccagc gccgatgacc gcgagttttt tcggcacgat
     2341 gtcgatatcg agcgccccga cgttgtcgca gacgatctcg ttatcgaccg ggacaccggg
     2401 caagtgacga gccttggacc cggtggcgac gatcacctgc ttcgcctcga ccaactcatc
     2461 gccaacacgc aattgccaga cctcacttcc ggcatccccg ttacgtccaa caaaagcgcc
     2521 gtggccgttc agcagggtga ccttgttctt cttgaacaga cccttgatgc cgctggtcag
     2581 ttgtttgacg atgccgtcct tgcgccccac catcttcgct acatcaatag tcggcgtacc
     2641 gaccgaaata ccctgctcgg cgaagccgtg tccggcttcc tcgaagacgt gcgaggtgtg
     2701 caacaaggct ttcgacggga tgcaaccaac gttcagacag gttccgccaa gacgcggttc
     2761 gccctttgga tctgcatagg cattcgattc acagcaggcg acattgaaac cgagctgcgc
     2821 ggctcgaatg gccgcaatat agccacctgg accagcaccg acaacaagaa cgtcaaattg
     2881 cttggacatg ataaattccc tttctaaccg cagagacaca aaggcacgga ggccgcacag
     2941 agaagtccct ctgtgtgtct ccgtgccccg gtgatgagtg gttaaacgcc gagcagcagg
     3001 cgcgctggat cttccaacgc ctctttcatc gtgaccagac cgagtaccgc ttcgcggcca
     3061 tcgatgatgc gatggtcata cgacatggcc agatagttga tcgggcgaat cacgatctgg
     3121 ccattctcga cgaccggacg gtctttggtg gcatgaatgc cgaggatcgc agactgcggg
     3181 gggttgatga tcggcgtaga cagcatcgag ccgaaaacac cgccgttcga gatcgagaag
     3241 gtgccacctg tcagatcttc catggacagc ttgccatcct tggctttaac accaaactcg
     3301 ccaatcttct tctcgatgtc ggcgatcgtc atctggtctg cgtcacgcag aatcggaacg
     3361 acgagaccgc gcggactacc cacggcgatg ccgatgtcga tatagccatg atagacgata
     3421 tcgttgccat ccaccgaagc gttgatcacc gggaatttct gaagcgcagc aacggcagcc
     3481 ttgacgaaga aacccatgaa accaaggcga acgccatgcg ccttctcgaa gcggtcgccg
     3541 tactgcttgc gcagcgccat gattggcccc atgttcactt cgttgaaggt ggtcaggatc
     3601 gcgttgctgg cctgtgactg cagcaggcgc tcggcaaccc gggcgcgcag gcgggacatc
     3661 ggcacgcgct gctccgggcg atcacccaac ggaaccgtca ctgtcggggc cggggcgagc
     3721 accgcagcag tgctcgcggc cggtgccgga gcggcagccg ccgccggttt tactgccgga
     3781 atctgggcgg cgagcgcatc ggctttggtc acacgtccgc cgcgcccggt acccgcgacg
     3841 tcactcgcgg cgacaccttt ctcttccagg atcttgcggg cggccggcag agccacgccg
     3901 gacgcagtcg ccacggcggc cgctgtgggc gcgacggtcg cttcgggcgt ggttgcaacg
     3961 ggcttggcag cagccgcggc aactgtgccg gcctgcgctg cggtgtcgat gcgtgcaatc
     4021 acctcgcccg cgacaacggt ttcgccatcg cttttgacaa tctcaaccaa cacgccgcta
     4081 tccggtgccg gcagttcgag gacgaccttg tcggtctcga tgtcgatcag gttatcatcc
     4141 cgcgcgaccg cttcgccggc cttcctgtgc caggacacca gggtagcttc ggcgacggac
     4201 tcggaaagct gtggaacttt gacgtcgatg atcatgttct ctccgttcta gggcagtttg
     4261 tagtactcga tctcaccaaa tgcggattca atcagagcct tttgttgggc gttgtgcttg
     4321 ctatggtagc cgacagccgg ggaagccgat gcgggccggg aaacaagcag gagatgctgc
     4381 ttgtcgctga gcgaccgcga caggtgctga cgcgaggcga tccagtacca ggctccctga
     4441 ttgcgcggct cttcctgaca ccagacgacc tcggtggctt tcggatactt cgcaagctcc
     4501 tcctggaacg actcctgcgg gaagggatag aactgttcaa gacgagcgat cgcaacatgc
     4561 gaaatcttcc tctcgtgcct tgcagccacc agttcgtaat agaccttgcc ggaacagaag
     4621 acgacacgcg tcactttctt ggcgttgatc ggctcgacct cgccgatcac cgggcggaat
     4681 tgaccttcgc tcaactcttc cagcgtcgac gtcgcatcct tgtggcgca
//
LOCUS       DAOSIT010000130         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16145_length_4709_cov_9.510743, whole genome shotgun sequence.
ACCESSION   DAOSIT010000130 DAOSIT010000000
VERSION     DAOSIT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16145_length_4709_cov_9.510743"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLL14_04440"
     CDS             complement(<1..313)
                     /locus_tag="PLL14_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HOG03202.1"
                     /translation="MKAVIAFVALAFAAGSTFAAEEKKEPSTAQKAQQEKMKACNAQA
                     AEKELKGDERKQFMSTCLGAKPAKVTQQEKMKACNGEAGSKQLKGDERKQFMSECLKA
                     NP"
     gene            complement(361..1305)
                     /gene="hprK"
                     /locus_tag="PLL14_04445"
     CDS             complement(361..1305)
                     /gene="hprK"
                     /locus_tag="PLL14_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOG03203.1"
                     /translation="MNTRRQFSVAQLYDDHREKLKLSWVVAVGVDRQIELKDQGYFGA
                     DVVGHLNLIHPERLQVIGRAETAWGCRVTADRLRYQVRELMAARPPALIIADDVEILP
                     AIREACESTATPLFVSPKPCSAVIDLLRIYLSRRLAGSTSVHGVLMDVLGMGVLITGD
                     SGVGKSELALELISRGHGLVADDVVELSCIAPTTIEGRCPSMLRDYLEVRGLGLLNIR
                     TIFGETASRRKMKLKLIVHLQKPLSSADGPRLPLDAQSQEILGVPIRKVVIPVAAGRN
                     IAVLLEAAVRNSILQLRGIDSMGDFINRQQQALLDDDL"
     gene            complement(1308..1754)
                     /gene="ptsN"
                     /locus_tag="PLL14_04450"
     CDS             complement(1308..1754)
                     /gene="ptsN"
                     /locus_tag="PLL14_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HOG03204.1"
                     /translation="MSQITQLLPLENIILDLDASSKKRVFEHVGLLFENYLGIARSTV
                     FDSLFAREKLGSTGLGQGVAIPHGRIKGLKDATGAFLRLAAPVAFDSPDGKPVSLLFV
                     LLVPEVATEQHLQILSELAQHFSDRNCREALTKAADASVIRQIFAG"
     gene            complement(1856..2182)
                     /gene="raiA"
                     /locus_tag="PLL14_04455"
     CDS             complement(1856..2182)
                     /gene="raiA"
                     /locus_tag="PLL14_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG03205.1"
                     /translation="MNLQVSGHHLEITPALHDYVTGKLERITRHFDNVIDVNVILSVD
                     KLKQKAEVTVHLAGKDVYVESVDEDLYAAVDGLVDKLERQVQKYKQKLQDHHRGNKIT
                     DHIVAE"
     gene            complement(2199..3623)
                     /locus_tag="PLL14_04460"
     CDS             complement(2199..3623)
                     /locus_tag="PLL14_04460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG03206.1"
                     /translation="MKPSLQLKLSQHLALTPQLQQSIRLLQLSTLELEQELEKYLLDN
                     PLLERQEDYRTPETPRDEGNVDAPAEAGAAAEQGEHSQAEDDSWLGDQAVTAGSSGSF
                     DDDDDGDGDYQNIQAATVSLREHLLAQLGLMTLSDRDRVLVRCLVEALDDDGYLTQSL
                     SELSEAMPEELEIAPEELQIALRHLQHFDPTGVGARTAQECLALQLEVLPADQTRSLA
                     MQIVGQHLELLASHDFPRLKKHTGCDDDALRAAHLLICSLNPRPGAAYASPDTRYITP
                     DVVVRKMRGQWLVNVNSDAFPRLRINSLYAQILARQRGSGLAGQLQEARWLIRNVQQR
                     FDTILRVAQAIVERQRQFLDHGEVAMRPLVLREIAEVLGLHESTISRVTTQKYMATPR
                     GIFELKYFFGSHVSTDAGGACSATAIRALIKQMISAEEPKKPLSDSQISEVLGQQGIV
                     VARRTIAKYREALSIPPVNLRKSI"
     gene            complement(3704..4438)
                     /gene="lptB"
                     /locus_tag="PLL14_04465"
     CDS             complement(3704..4438)
                     /gene="lptB"
                     /locus_tag="PLL14_04465"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOG03207.1"
                     /translation="MPDQQSRLSTRKLNKRYKSRTVVHDVSFDVVSGEVVGLLGPNGA
                     GKTTCFYMVVGLIACDGGDVHLDNVQLTHLPIHKRARLGVSYLPQEASVFRKLTVSEN
                     IKAVLELQNLSADEIDSRTESLLEELHISHLRISPATSLSGGERRRVEIARALATGPR
                     FILLDEPFAGVDPIAVLDIQKIIRFLKERGIGVLITDHNVRETLGICDHAYIINDGSV
                     LAAGRPEEIIYNDDVRKVYLGENFRL"
     gene            complement(4443..>4709)
                     /locus_tag="PLL14_04470"
     CDS             complement(4443..>4709)
                     /locus_tag="PLL14_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOG03208.1"
                     /translation="SRGIRLGDARLAYELRSRGVADELVSASLAAGEDELTRARRVWQ
                     KRFGSSPHGADDTAERARQMRFLAARGFSAETIRRLLRAAVEDD"
BASE COUNT          920 a         1377 c         1403 g         1009 t
ORIGIN      
        1 tcgggttggc cttgaggcac tcgctcatga actgcttgcg ctcgtcgccc ttgagttgct
       61 tgctgccagc ttctccgttg caggctttca tcttctcctg ctgggtcact tttgccggct
      121 ttgccccgag acaggtgctc atgaactgct tgcgctcgtc gcctttcagc tccttttctg
      181 cggcctgcgc gttgcaggct ttcatctttt cctgctgcgc tttctgggcg gtcgagggtt
      241 cctttttctc ttcggctgca aaagtgctgc cggcagcaaa agcaagggcg acaaaggcga
      301 tcacggcttt catcggaagt ttctcctgag ggttgaggaa gtctgctgat taagccactg
      361 ctacaggtcg tcgtcaagga gcgcttgctg ctgccggttg atgaagtcgc ccatgctgtc
      421 tatgccacgc agttgcagaa tgctgttgcg caccgccgcc tcgagcagga ctgcgatgtt
      481 tcgcccggcc gcgaccggga tcaccacctt gcgtatgggt accccgagga tctcctggga
      541 ctgcgcatcg agtggcaggc gaggaccatc ggcgctgctg agcggctttt gcaggtgcac
      601 gatcagcttg agtttcatct tccggcgcga tgcggtttca ccgaagatgg tgcggatatt
      661 gaggaggccc aggccccgca cttcaagata atcgcgcaac atgctcggac agcgtccttc
      721 gatcgtcgtc ggggcgatgc acgacagttc gacgacgtcg tcggcaacca ggccatgacc
      781 acgagagatc aactccagcg ccagttcgct cttgccaacg ccggaatcgc cggtaatcag
      841 gacgcccatg cccagcacgt ccatcaagac gccgtgcact gatgtcgagc cggccagacg
      901 acgggaaaga tagatgcgca ataggtcaat caccgccgag cagggcttcg gactgacaaa
      961 caggggcgtt gcggttgact cgcaggcctc gcgaatcgcc ggcaggattt caacgtcatc
     1021 agcaatgatc agcgccggtg gcctggcggc catcaactcc ctgacctggt agcgcaagcg
     1081 atcggccgtt acccggcaac cccaggcggt ttccgcccga ccaatcacct gcagtcgttc
     1141 gggatgaatc aggttgaggt gcccgacgac atcagcaccg aaatagccct gatccttgag
     1201 ctcgatctga cggtcgacac cgacggcgac gacccatgaa agcttgagct tttcacggtg
     1261 gtcgtcatag agttgcgcga cgctgaactg acgccgggtg ttcatcttca gccggcaaaa
     1321 atctgacgaa tcacactggc gtccgcggcc ttggtcaaag cttctcgaca gttgcggtcg
     1381 gaaaagtgtt gcgccagttc ggacaggatt tgcaggtgct gttcggtcgc cacttcgggc
     1441 accagcagga cgaacagcag tgacaccggt ttcccgtccg gggaatcaaa cgccaccggt
     1501 gcggccaggc gcaggaaggc gccggtcgca tccttgaggc ctttgatacg tccgtgcgga
     1561 atggcgacac cctgaccaag accggtagag cccagctttt cgcgtgcaaa caggctgtcg
     1621 aacaccgtgc tgcgtgcgat gccgagatag ttttcaaaga gcagcccgac atgttcaaag
     1681 acgcgttttt tgctgctcgc gtccagatca agaatgatgt tttctagggg cagtagttgg
     1741 gtgatctggc tcatgcgaca aaaacccgaa aaggcggacg tggaagaatg caaaaaagga
     1801 agcgcacgga gcgagggtct gttgccgctc agtccgtgcg cagactatac caacttcact
     1861 cggcgacgat gtgatcagta atcttgtttc cgcgatggtg gtcctgcagt ttctgcttgt
     1921 acttctgcac ctgccgctca agcttgtcta ccaggccatc caccgctgcg tacagatcct
     1981 cgtcgacaga ctccacgtaa acatccttgc cagcgagatg aacggtaact tcggccttct
     2041 gcttcagctt gtccaccgac agaatcacgt tgacgtctat cacgttatcg aagtgacgag
     2101 tgattcgctc gagcttgccg gtgacgtaat cgtgcagcgc cggggtgatc tcgaggtggt
     2161 gaccactgac ttgcaggttc atgatgtggc tccttttgct aaatggactt gcgtaagttg
     2221 accggcggga tgctgagcgc ttcacggtat tttgcaattg ttcgccgtgc gacaacaatg
     2281 ccctgctgac caagaacctc ggatatctgg ctatccgaga gaggcttctt aggctcttct
     2341 gcgctgatca tctgcttgat cagcgcccga atggccgttg ctgaacacgc cccgccagca
     2401 tccgtgctga cgtgactgcc aaagaaatac ttcaactcaa agatcccacg cggtgttgcc
     2461 atgtacttct gggtcgtgac ccgcgatatg gtcgattcat gcaatccgag cacctcggcg
     2521 atttcacgca gtaccaaggg tctcatcgcg acttcaccat gatccagaaa ctgacgctga
     2581 cgttccacaa tagcctgcgc tacacgcagg atcgtgtcaa accgttgctg tacattcctg
     2641 atcagccagc gggcctcctg gagttgacca gcgaggccag aaccgcgctg cctggccaga
     2701 atctgggcgt agaggctatt gatccgcagt cgcggaaagg cgtcggaatt gacgttgacc
     2761 aaccattgcc cgcgcatttt gcgcaccacg acatcgggtg tgatgtagcg ggtgtcgggc
     2821 gaggcataag cggctccggg ccgcggattc aggctgcaga tcagcaaatg cgctgcgcgc
     2881 agtgcatcgt catcacaacc ggtatgtttc ttgagtctcg ggaaatcgtg gctggccaac
     2941 agttcgagat gctggccgac gatctgcatc gccagcgagc gtgtctggtc agcggggagc
     3001 acttccagtt gcagcgcgag gcattcctgg gcggtgcggg cgccaacgcc ggtggggtcg
     3061 aaatgctgca agtgcctgag cgcgatctgc agttcttccg gcgctatttc cagttcctcg
     3121 ggcatcgctt ccgagagttc cgagagtgac tgggtaaggt agccgtcatc gtctagagcc
     3181 tcgaccagac atcgcaccag gacgcggtca cggtcggaga gagtcatcag gcccagttgc
     3241 gccaacaggt gctcgcgcaa ggagaccgtt gccgcctgga tgttctgata atcgccgtcg
     3301 ccgtcgtcat cgtcgtcgaa ggaacctgac gaacccgcag taacggcctg gtcgcccagc
     3361 caactgtcgt cctcggcctg ggagtgctcg ccctgctcgg cggcggcgcc cgcttcggct
     3421 ggggcgtcca cgttgccctc gtcgcgcggc gtttcgggcg tgcggtagtc ttcctggcgt
     3481 tcgagcagcg ggttgtccag caaatacttt tccagctcct gctcaagttc aagcgtcgac
     3541 agttgcaaca atcggatcga ctgttgcaac tgcggggtca atgcaagatg ctgggagagc
     3601 ttgagctgga gggatggttt catctacgga accgggtaag ccgccgcttt ggcatggaaa
     3661 actccccgca tggttctcga attatggctg caggccgttg ggttcagagg cggaaatttt
     3721 cacccagata caccttccgg acatcgtcat tataaatgat ttcctccggc cgaccagcgg
     3781 caaggacgct accgtcgttg atgatgtacg cgtggtcaca aatgcccagc gtttcgcgaa
     3841 cgttgtggtc ggtaatcagc actccgatgc cgcgttcctt gagaaaacgg atgattttct
     3901 ggatgtcgag cacggctatc gggtcgacgc cggcgaaagg ctcgtcaagc agaatgaaac
     3961 gcggtccggt ggcgagagca cgggcgatct ccacgcgtcg ccgttcacca ccggaaaggg
     4021 aggttgcggg gctgatgcgc aggtggctga tgtgcagttc ttcgagcaga ctttccgttc
     4081 ggctatcgat ttcgtcggcc gacaagttct gcagttcgag taccgccttg atgttttcgc
     4141 ttaccgtcag cttgcggaaa acggaagcct cctgcggcag atacgaaaca cccaggcgcg
     4201 cgcgcttgtg aatcggcaaa tgtgtcagct gcacattgtc gagatgaacg tcaccgccat
     4261 cacatgcgat caggccgacc accatgtaga agcaggtggt cttgccggca ccgttcgggc
     4321 ccagcaatcc cacgacttcg ccgctgacca cgtcaaagga aacgtcatgc actaccgtgc
     4381 gcgacttgta gcgcttgttg agcttgcggg tggacaatct gctctgctgg tctggcatgg
     4441 gttcaatcat cttcaacagc ggcgcgcaac aggcgccgaa tggtttctgc ggaaaagccc
     4501 cgagcggcca ggaagcgcat ctgccgggcg cgttcggcgg tgtcgtcggc gccatgcgga
     4561 ctactgccga agcgcttctg ccagacgcgc cgcgcccgtg tcagttcgtc ctcgccggca
     4621 gccaggctgg cactcaccag ttcgtctgca actcccctgg aacggagttc ataggccagg
     4681 cgtgcgtcgc ccaaacggat accccggga
//
LOCUS       DAOSIT010000131         4692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16230_length_4692_cov_8.024801, whole genome shotgun sequence.
ACCESSION   DAOSIT010000131 DAOSIT010000000
VERSION     DAOSIT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16230_length_4692_cov_8.024801"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLL14_04475"
     CDS             complement(<1..1067)
                     /locus_tag="PLL14_04475"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOG03209.1"
                     /translation="MKEHLVIFDTTLRDGEQSPGASMTKEEKLRVARQLERMRVDVIE
                     AGFAAASPGDFDAIQSIAKIIKGATVCSLARSNENDIRRAGEAIAPAARGRIHTFIAT
                     SPIHMEKKLRMAPDQVVEAAVKAVHWAREYTEDVEFSAEDAVRSDVDFLCRVFTAVIA
                     AGARTINVPDTVGYSVPSVWGELMRTLIERVPGAEQVVWSTHCHNDLGMAVANSLSAV
                     LAGARQVECTINGLGERAGNASLEEVVMAVRTRSDLFPVQTRIDTTQIVPASKLVSQI
                     TGYPVQPNKAVVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWAQNKLVLGKHSGR
                     NAFKSRLAALGIVFDSEESLNA"
     gene            complement(1171..1944)
                     /gene="pssA"
                     /locus_tag="PLL14_04480"
     CDS             complement(1171..1944)
                     /gene="pssA"
                     /locus_tag="PLL14_04480"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOG03210.1"
                     /translation="MPEIKPRKTLFNPELRRRGIYLLPNLFTTAALFCGFFAIVQAMM
                     GDFERAAVAIFVAMVLDGLDGRVARMTRTQSAFGAEYDSLSDMVSFGVAPALVTYAWA
                     LRDMGRLGWIAAFIYCVGAALRLARFNTTLEVTDRRYFQGLPSPAAAALVTGFFWVMI
                     DKHVAAADVRWFACGLVIFAGISMVSNIRFYSFKDVNLRRSVPFIFVVAIALGFAVLS
                     YDTPIALFGLFVAYGLSGYILALLHWCRRKPPSPPASAV"
     gene            complement(2022..3038)
                     /gene="ilvC"
                     /locus_tag="PLL14_04485"
     CDS             complement(2022..3038)
                     /gene="ilvC"
                     /locus_tag="PLL14_04485"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOG03211.1"
                     /translation="MKVFYDKDADLSLIKGKKVTIVGYGSQGHAHAQNLSDSGVKVTV
                     GLRKDGASWSKAENAGLKVEEVARAVRGADVVMMLLPDESIPQVYNAEVAPNMKKGAA
                     LAFAHGFNVHYNQVIAREDVDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVHQDRSGKA
                     RDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELAKMGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEVINETSRAAMRA
                     ALKRIQNGDYAKMFILEGRTNYPAMTARRRLIAQHEIETVGAKLRDMMPWIKKNRLVD
                     QSKN"
     gene            complement(3068..3559)
                     /gene="ilvN"
                     /locus_tag="PLL14_04490"
     CDS             complement(3068..3559)
                     /gene="ilvN"
                     /locus_tag="PLL14_04490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOG03212.1"
                     /translation="MRHIISILLENEAGALSRVAGLFSARAYNIETLTVAPTEDLSLS
                     RLTIVTSGTDAVIEQITKQLNKLIDVIKVVDLSEAAHIERELMLIKVRATGKDREELK
                     RLADIFRGHIIDVTESTYVIELTASGAKLDSFIQAIDAGLILETVRTGVSGIGRGERI
                     LKV"
     gene            complement(3572..>4692)
                     /locus_tag="PLL14_04495"
     CDS             complement(3572..>4692)
                     /locus_tag="PLL14_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG03213.1"
                     /translation="HLLLEAKRPMIYTGGGVILSNGAEQLTRLTRRLGFPITNTLMGL
                     GAYPGTDRQFLGMPGMHGTVEANLAMQHCDVLLAIGARFDDRVIGNPENYASIPRKII
                     HIDIDPSSISKRVKVDVPIVGNVPDVLEEMLRLLEVGNVSPDAAALGAWWKEIEEWRG
                     KLCMKYRMKKGVIMPQYVMEKLYEVTGGNAIVTSDVGQHQMWAAQYYKFDEPRRWLNS
                     GGLGTMGVGLPYAMGAALQHPDMQIACVTGEGSIQMCIQELSTAKQFRLPIKIINLNN
                     RYLGMVRQWQEMFYGSRYSESYMDSLPDFVKLAEAYGHVGIRVERPEDVEAALRKAFI
                     DHKDDLVFIDFITDQKANVFPMVAAGKGLADMILTEDL"
BASE COUNT          944 a         1451 c         1343 g          954 t
ORIGIN      
        1 gcgttcagtg actcctcgct gtcgaagacg atccccaatg ccgccaggcg tgacttgaag
       61 gcattgcgac ccgagtgttt gccaagcacc aatttgtttt gcgcccagcc gacatcctcg
      121 gcacgcatga tctcgtaggt ttcacggtgt ttcagcaccc cgtcctgatg aattccggat
      181 tcgtgcgcaa aggcgttggc gccgacgacg gccttgttgg gctgcaccgg gtaaccggta
      241 atctgcgaca ccagtttgga cgcgggaacg atttgcgtgg tatcgatccg ggtctgcacc
      301 ggaaacaggt cgctgcgcgt gcgtacggcc atcaccactt cttcgagtga ggcattgccg
      361 gcgcgttcgc cgaggccatt gatcgtacat tcgacctgac gcgcaccggc cagcacggcc
      421 gacagtgaat tggccaccgc catccccaga tcgttgtggc aatgggtgga ccacaccacc
      481 tgctcagccc ctggtacgcg ctcgatcagc gtccgcatca gttcacccca gaccgaggga
      541 acactatagc cgacggtatc gggaacgttg atcgtccggg cacccgcggc gatcaccgcg
      601 gtgaagacac gacaaagaaa atcgacgtcc gagcgtaccg cgtcttctgc cgaaaactcg
      661 acatcctcgg tgtattcgcg cgcccaatgc accgccttca ccgccgcctc gacgacctga
      721 tcgggcgcca tgcgcagttt cttctccatg tggatcggac tggtggcgat gaacgtatgt
      781 atccgcccac gggctgcagg cgcgatcgct tcgcctgccc ggcggatgtc gttctcgttg
      841 gaacgtgcca acgaacagac ggtggcgccc ttgatgatct tcgcaatcga ctggatggcg
      901 tcgaagtcgc cgggtgaggc cgcagcaaag ccagcctcga tgacatcgac ccgcatccgc
      961 tccagttgcc gtgcaacgcg cagtttttct tccttggtca tcgaggcgcc cgggctctgc
     1021 tcgccgtcgc gcaaagtggt atcgaatatt accagatgct ctttcatgtc ctgactccgg
     1081 atcgagggat ggaaataccc tgctgaaaca gtattgaatc gccgtgtcaa aggaggcgac
     1141 aaggcctccg gcgcggccgc ggcgggccgg ctatactgct gatgctggtg gcgatggcgg
     1201 cttgcgcctg caccagtgca gcagcgccag aatatagccc gagagcccgt aagcgacgaa
     1261 cagaccgaac agggctatcg gcgtgtcgta ggagagcact gcaaaaccga gcgctatggc
     1321 caccacaaag atgaaaggaa cgctgcgccg caggttaacg tccttgaaac tgtaaaagcg
     1381 aatatttgac accatcgaaa tgccggcaaa gatcaccagc ccgcaagcga accagcgaac
     1441 gtctgctgcg gcgacatgct tgtcgatcat gacccagaag aaaccggtca ccagcgccgc
     1501 cgcggccggg ctgggcaatc cctggaaata gcgccggtcc gtgacttcca gcgtcgtatt
     1561 gaagcgggcc aggcgcagtg ctgcaccaac gcaataaatg aaggcggcaa tccaccccag
     1621 tcgtcccatg tctctgagcg cccaagcata ggttaccaga gccggggcga caccaaaaga
     1681 caccatgtcg gacagggagt catattcggc gccaaaggcg ctttgtgtac gcgtcatgcg
     1741 cgcgacccgt ccgtcgagac cgtcgaggac catggccaca aagatggcca ccgcggcccg
     1801 ctcgaagtcg cccatcatcg cctggacgat ggcaaagaac ccgcaaaaca gcgccgcggt
     1861 ggtaaacaga tttggcagta gatagatgcc gcggcgccgc aattcgggat tgaacaatgt
     1921 cttgcggggc ttgatttcgg gcattgcgct gttgactcgg tgcagaggcg ggcgtgaggg
     1981 aagcgagcgc cgccctcagc ctcgccaggc catgaaagcg atcagttctt gctttgatcg
     2041 accagcctgt ttttcttgat ccagggcatc atgtcgcgca gtttggcacc gacggtctcg
     2101 atctcgtgtt gtgcaatcaa acgccgacgc gcggtcatcg ccgggtagtt ggtacgccct
     2161 tcgaggatga acatcttggc atagtcgccg ttctgaatcc gtttcagggc ggcacgcatt
     2221 gccgcacgcg aggtctcgtt gatcacctca ggcccggtca cgtactcgcc gtactcggca
     2281 ttgttcgaaa tcgagtagtt catgttggca atgccgcctt cgtacatcag gtcgacaatc
     2341 aacttcagtt catgcaggca ctcgaaatac gccatctccg gcgcgtaacc tgcctcgacc
     2401 agcgtttcaa agcccatctt ggccagttcg acggcgccac cgcagagaac ggcctgttca
     2461 ccgaacaggt cggtttccgt ctcctcgcgg aagttggtct cgatcacccc gcccttggtc
     2521 ccgccattgg ccgccgcgta ggacagcgcg atgtcacggg ccttgccgga gcggtcctgg
     2581 tgaacggcga tcagcgaggg gacgccacca cccttgaggt actccgagcg taccgtatgc
     2641 cccggcccct tcggggccac catgatgacg tccacatcct cacgggcgat cacctggttg
     2701 taatgcacat tgaagccgtg tgcgaaggcc aatgccgcgc ccttcttcat gttcggcgcc
     2761 acttccgcgt tatatacctg cgggatgctc tcatccggga gcagcatcat caccacgtcg
     2821 gcaccccgga cagccctggc aacttcctcg actttcaagc cggcattctc ggccttgctc
     2881 cacgaagcac catccttgcg cagaccgacc gtcaccttga cgccggaatc gctcagattt
     2941 tgcgcgtgtg catggccctg cgagccgtaa ccaacgatgg tgaccttctt gcccttgatc
     3001 agagagaggt cggcgtcctt gtcgtagaaa actttcatgc aatttcctct tttgctgttc
     3061 gctgtactta gacttttaga atacgctcgc cgcggccgat gccggatacc cccgtacgaa
     3121 cggtctcgag aatcagtccg gcgtctatcg cctgaatgaa ggaatcgagc ttcgctccgc
     3181 tcgcggtcag ttcaatgaca taggtcgatt cggtcacatc gatgatgtgg ccacggaaga
     3241 tgtccgccag gcgcttgagt tcctcgcggt ccttgcctgt agcgcggacc ttgatcaaca
     3301 tcaactcccg ctcgatatgc gcggcctcgg agagatcgac aaccttgatg acatcgatca
     3361 acttgttcag ttgcttggtg atctgctcga tcacggcgtc cgtccccgag gtgacgatgg
     3421 tcaggcgtga cagcgagaga tcttcggtcg gagcgaccgt cagggtctcg atgttgtagg
     3481 cacgcgccga aaagagacca gcgacccgcg acaatgcacc ggcttcgttc tccagcagga
     3541 ttgagataat gtgtcgcatc ttgcctttcc cttacagatc ttcggtcagg atcatgtccg
     3601 ccaggccctt gccggcggct accatcggga agacattcgc cttctgatcg gtgatgaaat
     3661 cgatgaaaac gagatcgtcc ttgtggtcga tgaatgcctt gcgcagcgcg gcctcgacat
     3721 cctcaggccg ttcgacgcgg atacctacgt gaccataggc ctcggcgagc ttcacgaagt
     3781 cgggcaacga atccatgtac gactccgagt accggctgcc gtagaacatc tcctgccact
     3841 gccgtaccat gcccaggtag cgattgttga ggttgatgat cttgatcggc aagcgaaact
     3901 gcttggctgt cgaaagctcc tgaatgcaca tctggatcga accttcaccg gtcacgcagg
     3961 caatctgcat gtccggatgc tgtagcgctg cacccatggc atagggcaga ccaaccccca
     4021 tggtaccgag gccacccgag ttgagccagc gacgcggctc gtcgaacttg taatattgcg
     4081 cggcccacat ttgatgctgg ccaacgtccg aggtgacaat cgcgttgccg ccggtcacct
     4141 catacagttt ctccatcacg tactgcggca tgatgacacc cttcttcatt cggtatttca
     4201 tgcacagctt gccccgccac tcctcgattt ccttccacca ggcccccaat gccgcagcat
     4261 ccgggctgac gttcccgacc tccagcagtc ggagcatctc ctcgaggaca tccggaacgt
     4321 tgccgacgat gggcacatcc accttgacgc gtttggaaat tgacgaggga tcgatgtcga
     4381 tgtggatgat cttgcgaggg atcgaggcgt agttctccgg attgccgatc actcggtcgt
     4441 caaaacgggc gccgatggcc agcaatacgt cgcaatgctg catggccaga ttcgcctcga
     4501 ccgtgccgtg catgccgggc atgccgagga attggcggtc ggtgcccgga taagctccga
     4561 gccccatcag ggtgttggtg atcggaaatc cgaggcggcg cgtgagtcgg gtcaattgct
     4621 cggcgccatt tgacagaatg acgccaccgc cggtatagat catcggccgc ttggcctcga
     4681 gcagcaggtg ca
//
LOCUS       DAOSIT010000132         4681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16271_length_4681_cov_11.545828, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000132 DAOSIT010000000
VERSION     DAOSIT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16271_length_4681_cov_11.545828"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLL14_04500"
     CDS             complement(<1..413)
                     /locus_tag="PLL14_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG03214.1"
                     /translation="MHLVLISGLSGSGKSIALKVLEDANYYCVDNLPSQLLQQLVGQL
                     DLQGYDKVGVVIDIRGGDSVAMLPLQLDALRANELDLQFLFLDAQDATLIKRYSETRR
                     RHPLASDRLTLTEAIAEERIRLGELGALGHHIDTSG"
     gene            497..1660
                     /locus_tag="PLL14_04505"
     CDS             497..1660
                     /locus_tag="PLL14_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOG03215.1"
                     /translation="MTLLNIPFLEELVTIRRDIHAHPELAFNELRTAELVAHELTRYG
                     LEVHSGLAGTGVIGVLRKGSGARAIGLRADMDALPLQEKNDFPHRSRDHGRMHACGHD
                     GHTTILLGAARYLSEHRDDLNFDGTVYFIFQPAEESEGGAAVMIADGLFDRFPMEAVF
                     GLHNWPGIPVGEMAVRPGPVMAGTCTFEVSVRGHGCHAAMPHQGVDTLVVSSQLVLAL
                     QTVVARNLHPCESAVVSVTQIHAGEAWNIIPDDAVLRGTIRSFNAETQEIVERAVERL
                     CTGIASAFGAQISVRFDHRYPPTVNSVAQSEVCRQVATALLGPDKVRTNELPSMGAED
                     FAYMLREKPGCYVWLGNGPGTGGCTLHNPHYDFNDEILPLGVNYWVRLVSAVL"
     gene            1897..>2016
                     /locus_tag="PLL14_04510"
     CDS             1897..>2016
                     /locus_tag="PLL14_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOG03216.1"
                     /translation="MRILLAEDDRIIADGLCRSLRQGGYAIDCAYNGIDADTAL"
     assembly_gap    2017..2116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2117..2371
                     /locus_tag="PLL14_04515"
     CDS             <2117..2371
                     /locus_tag="PLL14_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG03217.1"
                     /translation="NGEMLDLSARELGLLEIFLSRAKRLVSKDQLVDHLCGWGEEVSH
                     NAIEVYVHRLRKKLESTGVNIITVRGLGYYLEKPAEERKS"
     gene            2340..3836
                     /locus_tag="PLL14_04520"
     CDS             2340..3836
                     /locus_tag="PLL14_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOG03218.1"
                     /translation="MRNLRKRENPDLQRSLFGEILDWMLAPLLFVWPISIAFTHYFAN
                     SVASFPYDQSLREHVAAISRQISFVDGRAQLALPGLAQSFLRSDEIDNVYFHLLDHDG
                     HLIAGDPELPVPRKPEEFLNREHSEVLFRDDEYRGQALRIAYLYLFDASAPADLGVLI
                     EVGETTEKRSQLANKIIASVILPQFVIIPLAVVLVWFGLSQGLRPLTRLRDRIEARRE
                     GDLSPIALRRVPEELRPLTEAFNSMLTRMQHNVAAQRRFIADAAHQMRTPLTGLKTQA
                     QLAVRETDPAELRHALRQILMGADRASHLVDQLLSLARAEASGHSQQTLVALDLDHLL
                     REVVETWVVHALEKCIDLGYEPAGTVFIQGNAFLLREMVNNLLDNALRYTPAGGRVTA
                     RVVAQGDFALLEIEDSGPGISDEESQKVFDRFYRVEGTDSEGSGLGLAIVREIAEVHQ
                     GAASLRPNTRKVREDEPPGCVARIVFPVYRLQPPPVAIAETVRSGGFA"
     gene            complement(3903..>4681)
                     /locus_tag="PLL14_04525"
     CDS             complement(3903..>4681)
                     /locus_tag="PLL14_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOG03219.1"
                     /translation="SGTLLFERIGKRVFLTEAGEILLDHTRQILQSLKEADAALLALK
                     GLKGGRIAVAVASTAEYFAPGLLAEFRRTQADVRIRLLIDNRDTVSRLLAGNEVDVAI
                     MGRPPAELDAVAVSFAPHPLVIVASADHPLARRANVAVEDLAGETLIVRESGSGTRSA
                     MEEFFQERSIKPRIGMEMGSNEAIKQAVVAGLGISFISLHTLGLELSVGRLCILKVEG
                     TPVMRRWHIVRHRSKHLTPALAAFWDFVVEFAPAYLRRLV"
BASE COUNT          897 a         1425 c         1416 g          843 t
ORIGIN      
        1 ccactggtat cgatgtggtg accgagggca cccaactcgc caagccggat gcgttcttcg
       61 gcaatcgctt cggtcagcgt caagcgatcg ctggccaacg gatgtcggcg ccgcgtttcg
      121 gaatagcgct tgatcagggt ggcatcctgc gcatcgagaa acaggaactg cagatcgagt
      181 tcgttggcac gcagggcgtc gagctgcaac ggcagcatgg cgacgctgtc gccaccgcgg
      241 atgtcgatca ccacgcccac cttgtcgtag ccctgcaggt caagctggcc gacaagctgc
      301 tgcaacaact gcgagggcag gttgtcgacg caataatagt tggcatcctc aagaaccttg
      361 agggcgatcg atttgccgga accggacagg ccgctgatga gaacgagatg catgacctca
      421 aggataacgg aagcctggct ccccgggcaa gtcactctgg aataaacttg tagcttaccg
      481 ttgacaggcc ggtcttatga cacttctgaa cattcccttt ctcgaagaac ttgtgacaat
      541 tcggcgcgac atccacgcgc accccgaact cgcattcaat gaactgagga ccgccgaact
      601 ggtggcgcac gagttgacgc gctatggact ggaagtccat agcggcctgg ccggcaccgg
      661 tgtcatcggc gttctgcgca aggggagcgg cgcacgtgca attggcctgc gtgcggacat
      721 ggatgcgctg cctttgcagg agaagaacga tttcccgcac cgctcgagag accacggcag
      781 aatgcacgcc tgcgggcatg atgggcacac cacgatcctg ctcggtgccg cgcgctacct
      841 gagcgaacac cgcgatgacc tgaatttcga cggcacggtt tacttcatct tccaacccgc
      901 cgaggagtcc gagggaggcg ccgccgtaat gatcgccgac ggtctcttcg acaggtttcc
      961 aatggaggcg gtgttcggac tgcacaactg gcccggaatt ccggtgggcg aaatggcggt
     1021 gcggccaggg ccggtcatgg ccggcacctg caccttcgag gtttcggtcc gcggccacgg
     1081 ttgccacgcg gcgatgccgc atcagggggt cgacacgctg gtggtcagca gccagctggt
     1141 gctggccctg cagaccgtcg tcgcccgcaa cctgcaccct tgcgaatcgg cggtcgtcag
     1201 cgtcacccag atccacgccg gcgaggcgtg gaacatcatt cccgacgacg ccgttctgcg
     1261 cggcaccatc cgcagcttca acgccgaaac acaggaaatt gtcgagcgtg ccgtcgagcg
     1321 gctatgtact ggaatcgcca gcgctttcgg agcgcaaatc tcggtgcgct tcgatcaccg
     1381 ctatccgccg acggtcaaca gcgtcgcaca aagcgaggtc tgccggcagg tggcgacggc
     1441 cttgctcggt ccggacaagg tgcgcaccaa cgaactcccg tcgatgggtg ccgaagactt
     1501 cgcctacatg ctgcgcgaga aacccggttg ttatgtctgg ctgggcaacg gtccgggcac
     1561 gggcggctgc accctgcaca atccacatta cgacttcaac gacgagatcc tgccgctggg
     1621 agtgaattac tgggtccgcc tggtgagcgc cgtgctgtaa ggcatttttt gttacactcg
     1681 gggaggggtc aatggggtgc cgtgcttcgg ccacgaccag gccccgacgc cacaggtcca
     1741 aaaaatggcg gggagcgccc ggtgcagtat tcaggcgctc cccgagtttc ggccgaagcc
     1801 ggaactcccc agaccataaa aaggagaaca gcatgcgtag cttaaaggcg tggacttaca
     1861 aagaacttac gcgtaccaga tcgggaatcg cgggtcatgc gcattctcct cgctgaagac
     1921 gaccgcatca ttgccgatgg cctttgccgc tcgttgcgcc aggggggcta cgcgatcgac
     1981 tgtgcctaca acggcataga cgcagatacg gcactgnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnaatg gcgaaatgct cgatctctcg gcacgcgagc tggggctgct
     2161 cgaaatcttc ctgtcacggg cgaaacggct ggtcagcaag gaccagttgg ttgatcacct
     2221 ctgcggctgg ggtgaggaag tcagccacaa cgcgatcgag gtttacgtcc accgcctgcg
     2281 gaagaagctc gaatcgacag gagtgaacat catcaccgtg cgcgggctcg gctactatct
     2341 tgagaaacct gcggaagaga gaaaatcctg acctccagcg ctcgctgttc ggcgagattc
     2401 tggactggat gctggcaccg ctgctctttg tgtggccgat cagcattgcc tttacgcact
     2461 atttcgccaa cagtgtggcg agcttcccct acgaccagtc cttgcgcgaa cacgtggcgg
     2521 cgatatcacg ccagatcagt tttgtcgatg ggcgcgcgca actcgcgctg cccggcctgg
     2581 cacagagctt tctgcgatcg gatgagatcg acaacgtcta ttttcacctc ctggaccacg
     2641 acggccatct gatcgcgggc gacccggaac tgccggtacc gcgaaagccc gaggagtttc
     2701 tgaaccgcga gcacagcgag gtccttttcc gcgatgacga ataccgtggg caggccctgc
     2761 gcattgccta tctgtatctt ttcgacgcga gtgcgccggc cgacctcggg gtgctgattg
     2821 aagtcgggga aacgacggag aaacgctccc aactggcgaa caagatcatc gccagtgtca
     2881 tcctgccgca gtttgtgatc attccgctgg cggtggtgct ggtctggttc ggtttgtcgc
     2941 agggcttgcg tcctctgacc cgtctgcgtg accgcatcga agcccgccgc gaaggcgacc
     3001 tgtcgccaat cgccctacgc cgcgttccgg aagaattacg gccgctgacc gaagcgttca
     3061 attccatgct gaccagaatg cagcacaatg tcgccgccca gcggcgtttc attgccgatg
     3121 ccgcacatca gatgcgaact ccactcaccg gcctgaaaac acaggcgcaa ttggccgtgc
     3181 gcgagaccga tccggcagaa ctgcggcatg ccctcaggca aatcctgatg ggtgccgatc
     3241 gcgcgtcgca tctggtcgat cagttgctgt cgctcgcgcg tgccgaggcc agcgggcact
     3301 cgcagcagac gctggtagcg ctcgacctcg atcacttgct gcgtgaagtc gttgaaacct
     3361 gggtggtaca cgccctcgaa aaatgcatcg atctgggcta cgagcctgcg ggcacggtgt
     3421 tcatccaggg caacgccttt ctgctgcgcg agatggtcaa caacctgctc gacaatgccc
     3481 tgcgctacac gccggctggc ggccgggtga ccgcgcgggt ggttgcccaa ggcgatttcg
     3541 ccctgctcga aatcgaagac agcggaccgg ggatttccga cgaggagtca cagaaggttt
     3601 tcgaccgctt ttaccgtgtc gaggggacag acagcgaggg cagtggcctc gggctggcca
     3661 tcgttcggga aattgccgaa gtccatcagg gcgccgccag cctgcggccg aatacccgca
     3721 aggtgcgcga ggacgaaccg ccgggttgtg tcgccagaat cgtcttcccg gtttaccgcc
     3781 tgcagccccc accggtagcc attgccgaga ccgtccgcag cggcggattt gcctgaggac
     3841 ccatcattcg cccactttcc cggagcggct ggctcgcaca ccgggatgcg ctgcgtaggg
     3901 cctcacacca gccttctcag gtaagccggt gcgaactcga cgacgaagtc ccagaacgcg
     3961 gcaagagcag gggtgaggtg cttgctcctg tggcgaacga tgtgccagcg gcgcatgacc
     4021 ggcgtccctt cgactttgag gatgcacaga cgtccgacac tcagttccag ccccagggtg
     4081 tgcagcgaaa tgaagctgat gccaagcccg gcgacgaccg cctgcttgat cgcctcgttg
     4141 ctgcccattt ccatgccgat ccgcggcttg atcgaacgct cctgaaagaa ttcttccatc
     4201 gctgaacgcg tgcccgatcc cgactcgcgc acgatcaggg tttcaccggc aagatcctcg
     4261 acggcaacgt tggcccgcct ggccaggggg tggtcggcgc tggccacgat caccagcggg
     4321 tgcggcgcaa aggacaccgc caccgcatcg agttcggccg gcgggcgacc catgatcgcc
     4381 acgtcgactt cgttgccggc gagcaggcga ctgacggtgt cgcggttgtc gatcagcagg
     4441 cgaatgcgta catcggcctg tgttctgcga aattcggcca gcaaacccgg cgcgaaatac
     4501 tcggcggtac tggctaccgc gacggcaatg cgcccgccct tcagtccctt caaggccagc
     4561 agcgcggcat ccgcctcctt cagcgactgc agaatctggc gcgtatggtc gaggaggatt
     4621 tcgcccgctt cggtaagaaa aacgcgcttg ccgatccgtt cgaacagcaa ggtgccgctg
     4681 a
//
LOCUS       DAOSIT010000133         4670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16314_length_4670_cov_10.086457, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000133 DAOSIT010000000
VERSION     DAOSIT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16314_length_4670_cov_10.086457"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2547
                     /gene="hrpA"
                     /locus_tag="PLL14_04530"
     CDS             <1..2547
                     /gene="hrpA"
                     /locus_tag="PLL14_04530"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOG03220.1"
                     /translation="PRKAVSDGYQLLLELGAVSAERTLTATGRELAKMPLDPRLGRMI
                     VAARDEGCLREVLVIAAALSVQDPRERPQERQGTADAAHRKFADEKSEFLSWLRLWDW
                     YQAEFEHRKSQRKLVEACHENYLSALRMREWRDIHSQLHALVVEQQWHENQLPATYDA
                     VHRALLAGLLGNIGCKSEESAHYLGARGIKFLIHPGSALQKKAGKWLVAAEISETTRL
                     FARCVARIEPEWLERVGAHLLKRSWFDPHWEKKAMQAIAFERMTLYGIVVDPRRRVNF
                     GPLDPQAARELFIRQALVGGEVSDEFERRWGFFTHNQQLMLEIETLEHKSRRPDVLVD
                     DELIFAFYDGIVPEGIYSGASFDKWRRESERESPRLLFLQREDLMRHEAAGVTSEAFP
                     HQLRLAGVDYELGYHFEPGSARDGVTLTVPLAQLNQIPGARCEWLVPGLLKEKVLQLV
                     KTLPQRLRAKLVPVPDFAAEFVAQVQPADKPLVMALIAFILQSRGLNARGWEITPDAF
                     RPDALPAHLAFNFRLLDENGRQLAMSRSLSELRAEWGREAKQEFSELHETPGEYVGMR
                     DWTFGALPELMEVGLSGGQRVLGHPGLVDDGDSVSLTVFDSPEEARQAHRAGLLRLFM
                     LQFREPLKYLEKNLPGLTQMAMQFMTLGTLDELRRQLLDLTVARACLLDSWPTDAAGF
                     KTRCAEARPRLGLLAQEICRLVGQILSDWQSLQKKLPAFKAFDTTVQDVEGQLGRLIG
                     KRFIIETPFERLQHYPRYLKAIILRLDKLKAGGSAGALRDARLLAELAPLWGNYQRRA
                     SVLARQGVRDAQLEQFRWLLEELRVQLFAQELRTPVPVSSKRLQKMWEGM"
     gene            2581..3333
                     /locus_tag="PLL14_04535"
     CDS             2581..3333
                     /locus_tag="PLL14_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOG03221.1"
                     /translation="MTEVGLALLRSIKTLGKGRVWLLVFGPAVLGLLVWVGLAVFSLE
                     WLIASLVEQPPLSWLAGWGVLWLAKLAAVLGGWLLILAAAFVTAMLLAAIFVMPLLLE
                     VVSSTDYPELARLGKDSLAGAVVNSLVAVLVFTVGWLLTLPLWLIPGLALLLPVLWMA
                     WLTRRTFAYDALAMHATVDEWRLLRRQHGFPMLLLGVILALLAHIPVIGLLTPTLAAL
                     AYTHYCLEALRCLRQGAVVTVIGKQSTSKELQ"
     gene            3330..4088
                     /locus_tag="PLL14_04540"
     CDS             3330..4088
                     /locus_tag="PLL14_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOG03222.1"
                     /translation="MSFGAIIIGDEILSGKRIDRHFTRIAELLRVRGLRLAWAEYLGD
                     ERSRITTALQRSLAAGEVVFCFGGIGNTPDDHTRQAAAAALGVDLVLHPDAEREIRAR
                     FGAEINDVRLLLGTFPRGVDIIPNPFNRIPGFRVRDHHFLPGFPQMAHPMAEWVLDTY
                     YGHLFNQRPTVEEAFLLTGTNAYESALLGLMEHIVAEFPTLRLFSLPSISDDGQRRHL
                     ELGVEGDPTLVERAMATIRQEVERRGITWRSRPK"
     gene            complement(4158..>4670)
                     /locus_tag="PLL14_04545"
     CDS             complement(4158..>4670)
                     /locus_tag="PLL14_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOG03223.1"
                     /translation="SQKASAQVLLTLARTAFHGLEQLSALNLAASRELFNTGTTGTQQ
                     LLGVRDPQQLKDVAASLARPNVDKLLEYSRSLYDLSANLQREVTSVLEEQYSNIRHNA
                     AGLIEKTGATPIAGDVFGAAVKQLLNASNTAFENMSQMAKQVTDIVDTNVKAASNATA
                     QAAAALSPKK"
BASE COUNT          788 a         1376 c         1586 g          920 t
ORIGIN      
        1 ccgcgcaagg cggtcagcga cggttaccag ttgctgctcg aactcggcgc ggtcagtgcc
       61 gagcgtacgt tgaccgcgac cggccgggaa ctggcgaaaa tgccgctcga tccgcgcttg
      121 ggcaggatga tcgttgccgc gcgcgacgaa gggtgtctgc gcgaggtgct ggtgatcgcc
      181 gccgcgcttt cggtgcagga cccgcgcgag cggccgcagg agcggcaggg tacggccgac
      241 gcggcacacc ggaagttcgc cgacgagaag tcggaatttc tctcctggct gcggctctgg
      301 gactggtatc aggctgaatt cgagcaccgc aagtcgcaac gcaaactcgt cgaggcgtgt
      361 catgagaact acctctccgc gctgcgcatg cgcgagtggc gggacatcca cagccagttg
      421 cacgcactgg tcgtcgagca gcagtggcac gagaaccagt tgccggcgac ttacgacgcg
      481 gttcatcgcg cactgctcgc cggcttgctc ggcaacatcg gctgcaaatc ggaagagtcg
      541 gcgcattacc tgggtgcgcg cggcatcaag tttctgatcc atccgggatc ggcactgcag
      601 aaaaaggccg gcaagtggct cgtcgccgca gaaatcagtg aaacgacacg cctgtttgcc
      661 cgctgcgtcg cgcgcatcga acctgaatgg ctggagcggg ttggcgcgca cctgctcaag
      721 cgaagctggt tcgacccgca ctgggagaag aaggcgatgc aggcgatcgc tttcgagcgg
      781 atgaccttgt acgggatcgt cgtcgatccc cgccggcggg tgaacttcgg gccgctggac
      841 ccgcaggcag cgcgcgagtt gttcatccgc caggcgctgg tcggcggcga agtcagcgac
      901 gagttcgaac ggcgctgggg gttctttacc cacaaccagc agttgatgct ggaaatcgaa
      961 acgctggagc acaagtcgcg ccggcccgac gtgctggtag acgacgagtt gatctttgcg
     1021 ttctacgacg ggattgttcc tgagggcatc tacagcggtg cgagcttcga caagtggcgg
     1081 cgtgagagcg aacgcgaaag tccgcgtctg ctcttcctgc agcgtgaaga cctgatgcgg
     1141 cacgaagccg ccggcgtcac cagcgaggct tttccgcatc agttgcggct cgctggtgtc
     1201 gattacgaac tgggttacca cttcgaaccc ggcagcgcgc gtgacggcgt gaccctgacc
     1261 gtgccactgg cacaactgaa ccagatcccc ggcgcacgtt gcgagtggct ggtgccgggg
     1321 ctgctcaagg aaaaagtgct gcaactggtg aaaacgctgc cgcagcgctt gcgtgccaaa
     1381 ctggtcccgg tacccgattt cgccgcggaa tttgtcgcgc aggtgcaacc ggcggacaaa
     1441 ccgctggtga tggcgctgat cgccttcatt ctgcaatcgc gcgggctgaa cgcgcgtggc
     1501 tgggagatta caccggatgc gtttcgcccg gacgccctgc cggcgcacct cgcgttcaat
     1561 tttcgtctgc tggacgagaa tgggcgccag ctggcgatga gccgcagcct gagtgaactg
     1621 cgcgccgagt gggggcgcga ggcgaaacag gaattctccg agttgcacga aacccctggc
     1681 gagtatgtgg ggatgcggga ctggactttt ggtgccttgc cggaactgat ggaggtcgga
     1741 ctgagcggcg gtcagcgcgt gctcggtcac ccagggctgg ttgacgatgg cgacagcgtc
     1801 tctttgaccg tcttcgactc ccctgaagag gcgcgccagg cacaccgggc ggggctcttg
     1861 cggctgttca tgctgcagtt tcgcgaaccg ctgaaatacc tcgaaaagaa cttgccgggg
     1921 ctcacccaga tggcgatgca gttcatgacg ctcggaacgc tcgacgagct gcgtcggcag
     1981 ttgcttgatc tgaccgttgc gcgggcctgt ctgctcgact cctggccgac cgatgcggct
     2041 ggcttcaaga cgcgttgcgc cgaggcaagg ccacggctgg gcctgctggc gcaggaaatc
     2101 tgtcgtctgg tcgggcagat cctcagcgat tggcagtctc tgcagaagaa actcccggcc
     2161 ttcaaggcct tcgataccac cgtacaggat gtcgaaggac aattggggcg gttgatcggc
     2221 aagcgtttca tcatcgaaac accgttcgag cgtctgcagc attatccgcg ttatctcaag
     2281 gcgatcatcc tgcgcctgga caagctcaag gcgggaggta gcgcgggtgc cttgcgcgat
     2341 gcccgcctgc tggccgaact ggcgccgctg tggggcaatt accagcgacg cgccagtgtt
     2401 cttgcccggc agggagtgcg cgacgctcag ctcgaacaat tccgttggtt gctcgaagag
     2461 ttgcgcgtgc agttgttcgc tcaggaatta cgcacgccgg tgccggtatc gagcaagcgc
     2521 ttgcagaaga tgtgggaggg catgtgaacc tgtacccccg ctggccagag gagacggaac
     2581 atgactgagg ttgggctggc actgctgcgc agtatcaaga cccttggaaa ggggcgggtg
     2641 tggctgctgg tgtttgggcc agcggtgttg gggttgctgg tctgggtcgg gttggcggtt
     2701 ttttctctcg aatggctgat tgccagcctg gtcgaacagc cgccgctgag ctggctggcc
     2761 ggttgggggg tactctggct ggccaagctc gcggccgtgc tcggcggctg gctgctgatc
     2821 ctcgccgcgg ccttcgttac cgcaatgctg ctggcggcga tcttcgtgat gcccttgctg
     2881 ctggaggtcg tgtcatcgac cgactatccg gagttggcac gcctcggcaa ggacagtctg
     2941 gctggagcgg ttgtgaacag cctggtggcg gtgctggtgt ttactgtcgg ctggctgctg
     3001 accctgccgt tgtggctgat ccccgggctg gcgctgctgc tgccggtgct ctggatggcc
     3061 tggctgaccc gtcgcacctt tgcttacgac gcgctggcca tgcacgcgac ggttgacgag
     3121 tggcgcctgc tgcgccgcca gcacggcttt ccgatgttgc tgctcggcgt catcctggcg
     3181 ctgctcgcgc acattccggt cattggcctg ctgacgccga cgctggcggc tctggcttat
     3241 actcactact gcctggaggc gctgcgttgc ctaagacaag gggcggtggt cacggtgatc
     3301 ggcaagcagt ctacatcgaa ggagttgcaa tgagtttcgg ggcgatcatc atcggcgacg
     3361 agatcctgtc ggggaaacgt atcgaccggc acttcacgag aatcgccgag ctgttgcgag
     3421 tgcgcggctt gcgcctggcg tgggccgaat acctcggcga cgagcgctcg cgcatcacca
     3481 cggcgctgca gcgcagcctg gccgccggtg aggtggtgtt ctgctttggc ggtatcggca
     3541 atacacccga cgaccatacc cgccaggcgg cggcggcggc gctgggcgtc gacctggtcc
     3601 tgcatcccga tgccgaacgc gagatccgtg ctcgtttcgg cgctgagatc aacgacgttc
     3661 gcctgctgct cggcaccttc ccgcgcggcg tggacatcat tccgaacccg ttcaaccgca
     3721 ttcccggttt ccgcgtacgc gatcaccatt ttctcccggg ttttccgcag atggctcacc
     3781 cgatggccga gtgggtgctc gacacctact atgggcatct cttcaatcag cggccaacgg
     3841 tcgaagaggc tttcctgctg accggtacga atgcgtatga gagcgcgctg ctcggcctga
     3901 tggaacatat tgttgccgag tttccaacgc tacggctgtt cagcctgccg tcgatctccg
     3961 acgacgggca gcggcggcac ctggaactgg gcgtcgaagg cgatccgacc ctcgttgagc
     4021 gggcaatggc gacgatccgc caggaggtcg agcggcgcgg gataacctgg cgatcgcgac
     4081 caaagtaaac ccgccggaaa acaaaaaacc ccgaccagag ccggggtttc gagccgatca
     4141 ggtgcggcag aatctgatca tttcttcggg ctgagtgcgg cggcggcctg ggcggtggcg
     4201 ttgctggctg ccttgacgtt ggtgtcgacg atgtcggtca cctgcttggc catctgcgac
     4261 atgttctcga aggccgtatt cgaagcgttc agtagctgct tcaccgcggc gccgaaaacg
     4321 tcgccggcga tcggggtggc gccggttttc tcaatcaggc ccgctgcatt gtggcggatg
     4381 ttgctgtact gctcttccag cacggatgtg acttcgcgct gcagattggc cgacagatcg
     4441 tagaggctcc gcgagtactc caggagcttg tctacgttcg gacgcgccag cgaggcggca
     4501 acgtccttca actgctgtgg gtccctgacg ccgagaagct gttgcgtgcc ggtggtgccg
     4561 gtgttgaaca gttcgcggct ggcggcgagg ttaagggcgg acaactgctc cagtccgtgg
     4621 aaagcagtgc gagcgagagt cagcaacact tgggcactgg ctttttggct
//
LOCUS       DAOSIT010000134         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16339_length_4665_cov_10.640781, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000134 DAOSIT010000000
VERSION     DAOSIT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16339_length_4665_cov_10.640781"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /gene="lepB"
                     /locus_tag="PLL14_04550"
     CDS             <1..198
                     /gene="lepB"
                     /locus_tag="PLL14_04550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG03224.1"
                     /translation="RQDCSYNALGVICKVPPGHYFMMGDNRDNSRDSRFWGFVPEANI
                     VGKAFFIWLNFGDFKRIGSFK"
     gene            211..576
                     /locus_tag="PLL14_04555"
     CDS             211..576
                     /locus_tag="PLL14_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOG03225.1"
                     /translation="MNRQRGLALSALLVWGVVITLVAILVIRVLPEVMDYYKIRQAVK
                     AVAADSGGKTVAELRQAFGKYAEVEHIKTLAPAELDIFKEENQVVIAFAYEKRIPLAG
                     NVSLLIDFRGSSSGRNYGQ"
     gene            573..1244
                     /gene="rnc"
                     /locus_tag="PLL14_04560"
     CDS             573..1244
                     /gene="rnc"
                     /locus_tag="PLL14_04560"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG03226.1"
                     /translation="MTPSRLEQSLGYTFHDRALLLTALTHRSYSSPHNERLEFLGDAI
                     LNAVIARCLFKRYPMLPEGDLSRLRANLVRQDSLHQQALALALGDYLRLGEGEQKSGG
                     QQRPSILADALEALFGALWLDAGFDAAEVVVMRVYERVLDQLTPGQGIKDAKTRLQEH
                     LQGMRLALPKYTLAATEGEAHAQQFKVVCVIEAFKIHTEGRGANRRAAEQMAAERALE
                     ALEKR"
     gene            1241..2125
                     /gene="era"
                     /locus_tag="PLL14_04565"
     CDS             1241..2125
                     /gene="era"
                     /locus_tag="PLL14_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG03227.1"
                     /translation="MSELRSGQIAIVGRPNVGKSTLLNQLVGEKVSIVSQRAQTTRHR
                     IMGILTKPDAQYLFVDTPGFQTRHGNALNRVMNRGVRQALAEVDVVLFVVEAGRFDDR
                     DRAVRQLVPVDSPVIIVVNKIDQLKDKTQLLPYLARLATEGDFAAIVPVSAMRGAQLD
                     ALLAETRKHLPNQEFLFAEDEITDRSERFLAAEYIREKLFRLIGDELPYGAAVEVEKF
                     VVDGALRRISAAILVDREGHKSIIIGKGGEMVKRIASEARQDMERMFDGAVFLEVFVK
                     VKSGWADDERMLKTLGYD"
     gene            2164..2880
                     /gene="recO"
                     /locus_tag="PLL14_04570"
     CDS             2164..2880
                     /gene="recO"
                     /locus_tag="PLL14_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG03228.1"
                     /translation="MNQRHKVDGQPAFVLHAYPYSETSLIVDVFSRDHGRLALLARGA
                     RRPRSVMRGVLLAFQPLELGWAGRGEVHTLMKAEWQGGQPLLAGKSLFCGYYLNELLM
                     HLLPREDAHTRLFSVYADTLRRIADGAQESALRCFERALLQELGYGLTLESDAAGTAV
                     DADAHYAYEIERGPVRLSSPGSSTLAVSGRTLIDLAREDFSDPRSLAEAKQLMRTLIG
                     HYTGGKTLASRRIFRELQEL"
     gene            2877..3638
                     /locus_tag="PLL14_04575"
     CDS             2877..3638
                     /locus_tag="PLL14_04575"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOG03229.1"
                     /translation="MIELGVNIDHVATIRQARCTYEPDPVWAAVEAHLGGADGITIHL
                     REDRRHIQDADVERLRDLAQIKLNLEMAATDEMIAIACRIKPQMAMLVPEGRHEVTTE
                     GGLDIVAQETRLRQSVDRLAEAGIVSSVFINAEPAQVEAAARIGARVCEIHTGPYAHA
                     FYTQGRDAESRAVLAELDKVRSAGSLVRDLGMRFNAGHALNYFNVQPIAQLSGIRELH
                     IGHAIISRAVFVGLREAVREMKRLLREAQARAMRP"
     gene            3750..4127
                     /gene="acpS"
                     /locus_tag="PLL14_04580"
     CDS             3750..4127
                     /gene="acpS"
                     /locus_tag="PLL14_04580"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOG03230.1"
                     /translation="MIAGIGTDIVAVARLGSFYQRHGQRALEKLLSPRECTEFVGVRD
                     PARFLAKRFAAKEAFGKALGIGLVAPATLPSIGINHDALGRPLFEYATALAAHLAQRR
                     LIAHLSISDERDYAIAFVIMEQS"
     gene            4124..>4665
                     /gene="nagZ"
                     /locus_tag="PLL14_04585"
     CDS             4124..>4665
                     /gene="nagZ"
                     /locus_tag="PLL14_04585"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOG03231.1"
                     /translation="MNLPLGPLMIDLAGPQLSDLECERLCHPLVGGLILFSRNYESPQ
                     QLAQLTSAVHALRSPALPIGIDHEGGRVQRCRTGFTRLPAMRRLGELWDQDRQAALLA
                     ARRVAYVLAAELRACGVDLSFTPVLDLDWGRSSVIGDRSFHSDPTAVVELAGALIEGL
                     HRAGMIACGKHFPGHGWVEA"
BASE COUNT          870 a         1466 c         1468 g          861 t
ORIGIN      
        1 cgccaggatt gttcctacaa tgcgctcggc gtgatctgca aggtaccacc cggacactac
       61 ttcatgatgg gagacaatcg cgacaacagc cgcgacagtc gcttctgggg cttcgttccg
      121 gaagcgaaca tcgtcggcaa ggccttcttc atctggttga acttcgggga cttcaaacgg
      181 attggttcgt tcaagtaagg agcggaagct atgaatcgtc agcgcggtct ggcactttcg
      241 gccctgctgg tctggggcgt ggtgattacc ctggtggcca ttctcgtgat cagggtcctg
      301 cccgaggtca tggattacta caagatcaga caggcggtca aggcggtggc cgcagattcc
      361 ggcggcaaga cggtggccga acttcggcag gcgtttggca aatatgccga ggtcgagcac
      421 atcaagaccc tcgcgccagc ggaactggac attttcaagg aagagaatca ggtggtcatt
      481 gcttttgcct acgagaagcg cattcccctg gccggaaatg tcagcctgct gatcgacttt
      541 cgcggctcgt cgtcggggcg aaactacggc caatgacccc ttcccgactt gaacagtcgc
      601 tcggttatac gttccacgat cgtgcgctgc tgcttacggc gctgacgcac cgcagctata
      661 gttcccctca caacgagcga ctcgagttcc tcggtgacgc gattctcaat gcggtcatcg
      721 cgcgctgcct tttcaagcgc tatccgatgc ttcccgaagg cgacctgtcg cgactgcggg
      781 ccaacctggt gcgccaggac agcctgcacc agcaggcttt ggcactggca ctcggcgact
      841 atctgcgcct tggcgagggt gagcaaaaaa gcggcggaca gcaacggccg tcgattctcg
      901 ccgacgccct cgaggcgttg ttcggcgcgt tgtggctgga cgccggtttc gacgcggccg
      961 aagtagtcgt catgcgggta tacgaacgtg tccttgacca actcaccccc ggccagggta
     1021 tcaaggacgc caagacgcgt ttgcaggagc atctccaggg tatgcgactg gccctgccaa
     1081 aatacacgct ggccgccacc gagggcgagg cgcatgcgca gcagttcaaa gtcgtctgtg
     1141 tgatcgaagc cttcaagatc cataccgagg gccgcggggc gaaccgccgt gctgccgagc
     1201 aaatggccgc cgaacgagca ctggaagccc tggagaagcg atgagcgagc tacgatcagg
     1261 acaaatcgcc atcgtcggcc gcccgaacgt cggcaagtcg acgctattga accaactggt
     1321 aggcgagaaa gtcagcattg tctcgcaacg cgcgcagacg acccggcacc gcatcatggg
     1381 catcctcacc aagccggatg cccaatacct ctttgtcgat acgccgggtt ttcagacccg
     1441 ccatggcaac gcactgaatc gggtgatgaa ccgtggcgtc aggcaggcgc tcgccgaggt
     1501 ggatgtcgtc ctgttcgtgg tggaggccgg tcgctttgac gatcgcgacc gggccgtgcg
     1561 tcaactcgtg ccagtcgact ctccagtgat catcgtggtc aacaagatcg accagctaaa
     1621 ggacaagacc caactgctgc cgtatctcgc tcgacttgcc accgagggtg attttgcggc
     1681 gatcgtgccg gtcagcgcca tgcgcggcgc acaactcgac gccctgctcg ccgagacgcg
     1741 caagcacctg ccgaatcagg aattcctctt tgcggaggac gagatcaccg atcgcagcga
     1801 gagattcctg gccgccgaat acattcgcga gaagctgttt cgcctgatcg gcgacgagtt
     1861 gccttatggg gcggccgtcg aggtggagaa gttcgtcgtc gacggggctc tgcggcgcat
     1921 cagcgccgcg atcctcgtcg accgcgaggg acacaagtcg atcatcatcg gcaagggtgg
     1981 agagatggtc aaacgaattg cctccgaggc caggcaggac atggagcgga tgttcgacgg
     2041 tgcggttttt ctcgaagtct ttgtcaaggt caagtcaggc tgggccgacg atgagcgcat
     2101 gctcaagacg cttggctacg attagcgtgt ggactcgctg atggctggcg aacgagtcgg
     2161 gcagtgaatc agcggcacaa ggtcgacggt caaccggctt tcgttctgca cgcctacccc
     2221 tacagcgaga ccagcctgat cgtcgacgtc ttctcgcgcg accatggtcg tctcgcgtta
     2281 ctggcacggg gtgcccggcg gccacgctcg gtcatgcgcg gcgtattgct tgcctttcag
     2341 ccgctcgaac tggggtgggc aggccgcggt gaggtacaca cactgatgaa agccgagtgg
     2401 cagggcgggc aaccgctgct ggcgggcaag tccctgttct gcggctacta tctcaacgag
     2461 ttgctgatgc acctgctgcc tcgtgaagac gcgcacacac gactgttctc ggtctacgcc
     2521 gacaccctgc gtcgtattgc cgatggcgca caggaatcgg ccttgcgctg cttcgagcga
     2581 gcgctgttgc aggaacttgg ctacggtctg acgctggaaa gcgacgccgc cggaaccgcg
     2641 gtcgacgctg acgcgcacta cgcgtacgag atcgaacgcg gtccggtgcg actgtccagt
     2701 cccggcagtt cgacgctcgc ggtcagcggc agaacgctga tcgatctcgc gcgtgaggat
     2761 ttctccgatc cccgctcgct ggccgaagcc aagcaactga tgcgcacgct gatcggccac
     2821 tacacaggcg gcaagacgct cgccagccgc aggattttca gggagctaca ggagctatga
     2881 tcgaacttgg cgtcaatatt gaccatgtcg cgacgatccg ccaggcgcgc tgcacctacg
     2941 agccggatcc ggtctgggcc gcagtcgaag cgcacctcgg cggtgccgat ggcatcacca
     3001 tccacctgcg cgaggaccgg cggcatatcc aggatgccga cgttgaaagg ctgcgcgatc
     3061 tggcacagat caagctgaat ctcgaaatgg cagccaccga cgagatgatc gccatcgcct
     3121 gccgcatcaa gccgcaaatg gccatgctgg tgcccgaagg ccgccatgag gtgacgaccg
     3181 aaggggggct cgacatcgtg gcccaggaaa ctcgcctgcg ccagtccgtc gaccgcctcg
     3241 ccgaggccgg catcgtcagc agcgtattca tcaacgccga gcccgcccag gtggaagctg
     3301 ctgcgcgcat cggcgcacgg gtgtgcgaaa tccacaccgg accctatgcg catgcgtttt
     3361 acacccaggg aagggatgcc gaaagccggg ctgtgctcgc cgaactcgat aaagtccgca
     3421 gcgcaggcag cctcgttcgt gatctgggaa tgcgtttcaa tgccgggcac gcgctcaact
     3481 acttcaacgt gcagccgatc gcccagctgt cgggcatccg cgaactgcac atcgggcacg
     3541 cgatcatcag ccgggcggtc tttgtcggcc tgcgcgaggc cgtgcgcgaa atgaagcgcc
     3601 tgctgcgcga ggcacaggcg cgcgccatgc ggccatgacc ctgccggctt gcagatgatc
     3661 cgcttcatgc gcgttgccat gcaccgccgt ccgtcttccg caaacctacg cggggcgttt
     3721 ggcaagtcgt cggtgcgacg gtcacggcca tgattgccgg tatcggcacc gacattgtcg
     3781 ccgtggcccg cctcggcagt ttctaccagc ggcacgggca acgcgcgctg gaaaagctac
     3841 tgtcgcccag ggagtgcacc gagtttgtcg gcgtcaggga tccggcgcgc ttcctggcca
     3901 agcgttttgc ggccaaggaa gcgttcggca aggcgctcgg catcggcctc gtcgcacccg
     3961 ccaccttacc gagcatcggc atcaaccacg atgcgctggg caggccgttg ttcgaatatg
     4021 ctaccgcgct cgccgcacac ctcgcacagc ggcggcttat cgcccacctt tcgatcagcg
     4081 acgagcgcga ctatgcgatc gcttttgtca tcatggaaca gtcatgaacc ttcctctcgg
     4141 cccgctgatg atcgatctgg cagggcccca actgtccgac ctggaatgcg aacgcttatg
     4201 tcacccgctg gtgggtggcc tgattctgtt ctcgagaaac tacgaatcgc cgcagcagct
     4261 tgcgcagctg acgtccgcag tgcacgcctt gcgctcccct gccctgccga ttggcatcga
     4321 ccacgagggc ggcagggtgc aacgctgccg gaccggcttc acccgcctgc cggcgatgcg
     4381 ccgtctcgga gaactgtggg accaggatcg gcaggcggcg ctgcttgcgg cgcgcagggt
     4441 tgcctatgtg ctcgccgccg agctacgcgc gtgcggcgtc gatctgtcgt ttacgccggt
     4501 gctcgacctc gattggggcc gcagcagcgt catcggtgat cgttcgtttc actctgaccc
     4561 gaccgcggtc gtcgaactgg ccggcgcact gatcgagggc ctgcaccgcg ccggcatgat
     4621 cgcctgcggc aagcatttcc ccggccatgg ctgggtcgag gcgga
//
LOCUS       DAOSIT010000135         4649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16400_length_4649_cov_5.909882, whole genome shotgun sequence.
ACCESSION   DAOSIT010000135 DAOSIT010000000
VERSION     DAOSIT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16400_length_4649_cov_5.909882"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLL14_04590"
     CDS             <1..1074
                     /locus_tag="PLL14_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HOG03232.1"
                     /translation="IEALARLAEDTDTDKFRRIDRLMAEQARIDKEIDAIQKGQMRVL
                     PHTTALERTREIITLADDLAGDFRRVRDQFDQLNRDLRERIMDNDGNRGDVLDSLFAG
                     IDLISESDAGRTFSAFWRLLTDPEQAATLDQALDSVMSREFVGQLEAKERRFLLRLTR
                     TLLEQGGMVHKVLQTFARSLKHFVQSREYLEQRRLNHLLKDAQRAALTLKDEVRATET
                     LQYTLELTSSRLRSLSQWVLHDPSLQALPGQMAEGDAPPIDLESVSELVAQSEIDFRT
                     LKANVLAVLEQRSQASIAEVLGQFPAAQGLGSVVGLLALGSRHGFKVHHSETVGWVGG
                     DDERRSARIPKIFFLRERANELV"
     gene            1061..1780
                     /locus_tag="PLL14_04595"
     CDS             1061..1780
                     /locus_tag="PLL14_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOG03233.1"
                     /translation="MNWSKDVPVSDESAMPAEAQAPDAAQAPANTLFMGDSGELALDT
                     RRALVQLLAGPSLDGRRHPKLWPILVRDEAVIRRRLAELFLELVIDRDVQVAFTRQAD
                     TGDLEVPLLLRRAQLTFIDSILLLHLRQRLTQADSQGDRAVVSTDEITEFLSLYERAA
                     NTDRAGFVKRVHASIEKIKKHSILQKIRSSEDRFEISPTLKLLFSAEEIQALTHLYQR
                     MATGETPAQLAQTDVDEEADQ"
     gene            1777..4437
                     /locus_tag="PLL14_04600"
                     /pseudo
     CDS             1777..4437
                     /locus_tag="PLL14_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1032 a         1323 c         1416 g          878 t
ORIGIN      
        1 atcgaggcct tggcccgact tgccgaggac acagacaccg acaagttccg ccgcatcgac
       61 cggctgatgg ccgagcaagc ccgcatcgac aaggagattg acgccatcca gaaggggcag
      121 atgcgcgtgc tgccccatac gacggcgctc gagcgcacgc gggagatcat cacgctggcc
      181 gacgatctgg ccggggattt ccgccgagtc cgagatcaat tcgaccagct caatcgcgat
      241 cttcgtgaac gcatcatgga caacgatggc aatcgtgggg atgtgctgga ttcgctgttc
      301 gccggaatcg acctgatttc agagagcgac gccggaagga ctttctccgc tttctggcgc
      361 ttgcttacgg acccagaaca ggctgccacg cttgatcagg cacttgatag cgtgatgtca
      421 cgtgagttcg tcgggcagct cgaagccaag gagcgacgat tccttctgcg gctgacgcgc
      481 acccttcttg agcagggcgg catggttcac aaggtgctcc agaccttcgc gcgtagcctg
      541 aagcacttcg tccagagtcg tgaatacctc gagcaacgcc gactcaatca cctgctgaag
      601 gatgctcagc gcgcagcact gaccttgaag gatgaagtca gggccactga aaccctccag
      661 tacacgctgg aactcacgag cagtcgcttg cgctccttgt cccaatgggt actgcacgac
      721 ccatccctgc aagcattgcc tgggcagatg gcagaagggg acgcaccgcc aatcgaccta
      781 gagtctgtga gcgaactggt cgcacagtcc gagattgact tccgaacact gaaggccaac
      841 gtgctggcag tcctggagca acgctcccag gcatcgattg ccgaggtgct gggacagttc
      901 cccgctgcac aggggctagg cagcgtcgtc ggcctgctgg cgcttggcag tcggcatggg
      961 ttcaaggtcc accatagtga aactgtaggc tgggttggag gcgacgacga gcgtcgtagc
     1021 gcccgaattc caaagatctt ctttttgagg gagcgggcca atgaactggt ctaaagacgt
     1081 gcccgtaagc gacgaatcgg caatgcctgc agaagcacaa gcaccagatg ctgctcaggc
     1141 accggccaac accctgttca tgggtgacag cggtgagttg gcactggaca cccggcgcgc
     1201 cctggtgcaa ctgctggcag ggccgtcgct cgacggacgt cggcatccaa aactctggcc
     1261 gatcttggtg cgagatgaag cggtgatccg tcggcgtctt gccgaactgt ttctcgaact
     1321 ggttatcgac cgggacgtac aagtggcttt cacgcgccag gcagataccg gcgacctcga
     1381 agtgcctctc ctgctgcgtc gcgcccagct gacgttcatt gactccattc tgttgctcca
     1441 ccttcgccaa cgattgaccc aggcggactc gcagggcgac cgtgccgtgg tgtcgacaga
     1501 cgagatcacg gagtttctct ctctatatga gcgcgccgcc aataccgacc gggcagggtt
     1561 cgtgaaacgg gttcacgcct ccatcgaaaa gatcaagaag cacagcattc ttcagaaaat
     1621 tcgctcaagc gaggatcgtt tcgagatttc accgacgctg aaactcctgt tttcggcaga
     1681 ggaaatacag gcactgacgc acctgtatca gcgcatggcg accggagaaa cgcctgcgca
     1741 actggctcag actgacgttg acgaggaggc cgaccaatga tctcggaacc ccaaaccgcc
     1801 tctttgtttg cccgcgagca gttccggatg acccgcctgc aggtttacaa ctggggcact
     1861 ttttccggtc tgcacgacgt accaatcagc gagcgaggct ttctgtttgt cggacgatcc
     1921 ggcgctggaa agtcgacgct tctcgatgca ttttcggcgt tgctgacgcc gcctcgctgg
     1981 gtggacttca atgcggccgc acgcgaggcg gatcgcagtg gccgtgaccg aaacctcgtg
     2041 acctacatac ggggcgcctg ggccgaacaa aaggatgggg cgtcgggaga gatcgcaacg
     2101 cgctatctgc gctcagggac aacgtggtct gccctggctc tgagctacca gaacgccttg
     2161 ggacagtctg tggtgctggt tcaggtgttc tggctgcgtg gcaatgcgaa cggcaacacc
     2221 gacgtcaaac gccactacct tgtcatggag cgggcctttg atctgcgcga gttggaggac
     2281 ttcggccaat ccaacttcga catccgaaag ctcaagcagt ccttccctga ggcgtttgcc
     2341 cgcgatgaat ttcgtcccta ttgcgaacgg ttctgtcgcc tgctcggcat cgagagcgaa
     2401 atggcgctgc gtttgctgca caaaacccag tcggccaaga acctaggcga cctcaatacc
     2461 ttcctgcgcg acttcatgct cgacaagccc gagaccttcg aggtagcgga tcgcttggtc
     2521 agtgaatttg gcgaactcaa tgctgcccac caggcggtcg tcacggcccg cgagcaggtt
     2581 caaacgctcg cgccagcgcg ggagcagcat cagcgcaggg actcgttgat gctgcagcgc
     2641 aatggcttgg atgagcttcg gctgggcgtg gacgcctacc gggagacccg ccgcattgat
     2701 ctcctcaagc agcatgtcga atccctgcag gtgcaagcca gcggcactga aggcgaagtg
     2761 agtcgacgac aaagcacgct ggataaccac tccacgacct tgcgcgatct ggagcgacag
     2821 caccgtgaag caggcggtga tcagatcgag caatgggaag ccgagaaatc gggtctggag
     2881 ggccagcgca cagatcggct gcgcaaacgt ggtcaggccg aagaggcgtg caagaagctg
     2941 ggctggagcc tcccggattc gcctcaagcc ttcgcggaac tgttgggtga ctcgcggcat
     3001 gaggttgaaa actgggagca gcgcagcaac gaaaaccgcg aaacacagtt ccgattggca
     3061 ggtgaaaaaa gggacgccga aacagcgttt tcacaagtgg ttaaggaggt tcaggcactc
     3121 cagcgccagc catcgaacat tccggcggac atgctggaaa tgcgccgaag catcgccacc
     3181 gccatcgggg tctcggagtc ggtgctgcct ttcgttggcg aactcaccga ggtgaagccc
     3241 gatgaggccg aatggcaggg ggccatcgag cgtgtactgc acgggtttgc gctctctctc
     3301 ctggtagacg agcgccagta ttcggcattg gccaatcaca tcaacaccac ccaccttggg
     3361 cagcgcctgg tgtattaccg ggctggctgc ccggagacat ggcaggccaa gcccattggg
     3421 gccaattcgc tcgtcctcaa gctgaacgtc aaggagggta cgtatgccga ttggctcaag
     3481 gctgagctac ggcaacgctt cgattacgcg tgcgtcgatt ccattcagtc gttcaggagt
     3541 gctgatcgcg ccattacccg cgagggtcag gtcaagcaca gcaaaacccg gcatgagaag
     3601 gacgaccgca gaagtgtcgg tgaccggcgc aactgggttc tcggatttga taaccggaag
     3661 aagctcgagc tctttcaagc tcaggcgcag gagcaggctg acgcgattgc gcgcctcggc
     3721 ggggagatcg acaaactctc cgaccaagac aaaaatcgtg cgacccgagc gatgcagtgc
     3781 cagactctgg tgaatctcca gtggcaagaa atagatgtac tcccattgct ggatcgcatc
     3841 tccaccattg aacggcagat ccacgaagcg cgggagggca atacggcctt acagcaaatc
     3901 agtgagcgga tcgttaagca aaaggacctg gttgatcaag cagacaaggg gctccgagaa
     3961 gccaaggtcg cgcacgactc agttctcaag cagatcaacg acggcacgct gaagctggaa
     4021 accctccttc aagatgcttc cagcgttcca ttgactccgc atcagatcaa ggggctcgac
     4081 gaacgcttca ccaagcagtc ggactcggtc aggctcgaaa gcctcgacaa ggtagcaact
     4141 gctgttgaga gagggctcaa cacggaaatc ggtgagatca aagatgagat caccgattgc
     4201 gagaaggaaa tagaagcgcg ctttgcccat ttcatccgag aatggaaagc ggagtcgggt
     4261 ggtttggacg cgacgctagc ctctgcgcca gacttcttcg caaaattggt tcgactggaa
     4321 acggatggct tgcccgccta tgagcagcgg tttttcgagc tgctgcaaaa ccaaagccac
     4381 cagaatctgg tggcactttc cacctacctt aacgatgcgc gcaaagccat cctggatgag
     4441 tgtggtcacc tgatttgatg gtgcgatgtt cgtggttggt gatgggcatt tgtggggtag
     4501 gcaggatgcg caacgccggc aaaaggagcc ggcgttgtct ctggtggagt tgctggggga
     4561 gtcgtcagaa cggtatgtcg tcgggagggg tcacgggtgt gtattccacg caaactggac
     4621 agtgattcca cggcaaactg gacagtcgg
//
LOCUS       DAOSIT010000136         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16621_length_4594_cov_8.249174, whole genome shotgun sequence.
ACCESSION   DAOSIT010000136 DAOSIT010000000
VERSION     DAOSIT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16621_length_4594_cov_8.249174"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /gene="hyfB"
                     /locus_tag="PLL14_04605"
     CDS             complement(<1..1390)
                     /gene="hyfB"
                     /locus_tag="PLL14_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HOG03234.1"
                     /translation="MTAGGLLAATRWVHLDWVLLVVAAWLLIAVVGVLALRRLTLVAR
                     VLFPVGGAGGLALLGIALSALQGSPEVAVLPIGLPQLPFHFRLDSLSAFFLMVIGGIS
                     VGVSAFAAGYFRKGEGTPPGLLAFEYHLFLASMVLVVLADDAYAFMVMWETMALSSFF
                     LVTANHRIVEIQRAGYLYLLVAHIGAIGILLCFGVLQANSGDYTFANMRAQQLSPFWA
                     SLAFLLALFGFGAKAGILPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGVLRVLFDL
                     LQTPIWWWGLLLLTVGLATALFGVVFAAVQVDMKRLLAYSSIENIGLLFSGIGLAVLF
                     SAYGMQPMAALALTAVLYHVAGHAFFKSLLFVGTGAVLHATGERNLGKLGGLIRYMPW
                     VAWLMLLGVLASAGLPPFAGFVSEWLLLQSFLFSPGLPSAFLNMLIPVVAALIALIAA
                     LAGYTMVKFFGVI"
     gene            1604..1804
                     /locus_tag="PLL14_04610"
     CDS             1604..1804
                     /locus_tag="PLL14_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOG03235.1"
                     /translation="MENRTARLTILIDPRKKQIFEEICAAHDLTPSQVVRKLIRQYVI
                     DNAGDRELPDWLRARPSADRDG"
     gene            1947..3230
                     /locus_tag="PLL14_04615"
     CDS             1947..3230
                     /locus_tag="PLL14_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03236.1"
                     /translation="MKLETLAVHAGYSPEPTTKAVAVPIYQTSSYAFDSTQHGADLFD
                     LKVAGNIYTRIMNPTQDVLEKRVAAMEGGIAGLALASGQAAITYAIMTISEAGDNIVS
                     ASTLYGGTYNLFAHTLPQYGIEVRFANHADPASFERLINGKTKALYCESVGNPLGNIT
                     DFEQLAEIAHRHGVPLIVDNTVPSPYLCRPFEHGADIVVHSLTKYIGGHGNSIGGMIV
                     DSGRFPWAEHKLKFKRLNEPDVSYHGVVYTEALGPAAYIGRARVVPLRNMGAAISPLN
                     SFLILQGLETLPLRMDRICANTERIAAYLQGHAKVAWVNYAGLPDHPDHALVQKYMDG
                     KASGILTFGVKGTDCSGTEAGARFQDALQLFTRLVNIGDAKSLACHPASTTHRQLGAA
                     EMAKAGVSEEMIRLSVGIEHIDDLVADLEQALAAA"
     gene            3346..4257
                     /locus_tag="PLL14_04620"
     CDS             3346..4257
                     /locus_tag="PLL14_04620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG03237.1"
                     /translation="MSTSPYRLPLVDLRADDAEIGNYAALLDSLNIGLLVFDANGALR
                     LRNAHAEHLLGAGPGIWEDENGQPLVADDRLEKQVVHTRQPVRQRAFGIRRDPAGTRT
                     WCKASAFPVFSGKGSLRRVLLTLADLSQYSRLATENPPLPTHDPLTGVFSERYISLLL
                     DDEDRRARRYGTPFALALVTPDDYSEFCARHGAPAAASLLADVGRVLGRGLREFDMVG
                     RLGDEEFLLILPNVRVNDAMHGLERLREMVESSHPGDGEPALTISGGVTECTGEDAAV
                     LIERVRSLLASAREAGGNRLCVDPEIF"
     gene            complement(4282..>4594)
                     /gene="zapE"
                     /locus_tag="PLL14_04625"
     CDS             complement(4282..>4594)
                     /gene="zapE"
                     /locus_tag="PLL14_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HOG03238.1"
                     /translation="WFDFRTLCGGPRSQNDYLELARGYHTVLLSAIPRMSASMSSEAR
                     RFTWLVDIFYDHKVKLIATADCGPDSLYMQGTQASEFFRTASRLTEMRSREYLGLPHL
                     S"
BASE COUNT          946 a         1533 c         1407 g          708 t
ORIGIN      
        1 agatgacgcc gaagaacttg accatggtat agccggcgag cgcggcgatc agcgcgatca
       61 gggccgccac caccgggatc agcatgttca ggaaggcgct cggcaagccc ggcgagaaca
      121 ggaagctctg cagcagcagc cactcggaga cgaagccggc gaacggcggc aggccggcgc
      181 tggccagaac gccgagcagc atcagccagg ccacccaggg catgtaacgg atcagtccgc
      241 ccagcttgcc gagattgcgc tcgccggtgg catgcagcac cgcgcccgtg ccgacaaaca
      301 gcaggctctt gaagaaggcg tggccggcga catggtagag caccgcggtc agtgccagcg
      361 ccgccatcgg ctgcatgccg taggcggaaa acaggaccgc gagaccgatg ccggagaaca
      421 gcagaccgat gttctcgatc gacgaatagg ccagcaggcg tttcatgtcg acctgtaccg
      481 ccgcgaaaac gacgccgaac aacgccgtcg ccagtccgac cgtcagcaac agcaggcccc
      541 accaccagat cggcgtctgc aggaggtcga agagtacgcg cagcacgccg tagatcgcgg
      601 tcttcaacat caccccgctc atcagcgcgg acaccggcga gggtgcggcg ggatgcgcct
      661 cgggcagcca gacgtgcagc ggcaggatgc cggccttggc gccgaaaccg aacaacgcca
      721 gcaggaaggc cagcgacgcc cagaacgggg aaagctgctg ggcgcgcatg ttggcaaagg
      781 tataatcgcc gctgttcgcc tgcagcacgc cgaagcacaa caggatgccg atggcgccga
      841 tatgcgcgac cagcagatag aggtaaccgg cacgctggat ctcgacgatg cggtgattgg
      901 cggtgaccag gaagaacgag gacagggcca tcgtctccca catcaccatg aaggcgtagg
      961 cgtcgtcggc gagcaccacc agcaccatgc tcgccagaaa gaggtgatac tcgaaggcca
     1021 gcaaaccggg cggcgtgcct tcgcccttgc ggaagtagcc ggcggcaaac gccgacacgc
     1081 ccaccgagat gccgccgatg accatcagga agaacgccga caggctgtcg aggcgaaaat
     1141 ggaacggcaa ctgcggcaat ccgatcggca gcacggcgac ttctggcgaa ccctgcagcg
     1201 cactcagggc aatgccgagc agcgccaggc cgccggcgcc tccgactgga aacaggaccc
     1261 tcgccaccag cgtcaaacgg cgcagggcaa gcacgccgac caccgcgatc agcagccacg
     1321 cggcgacgac cagcagcacc cagtccagat gtacccaacg cgtcgctgcg agcaagccac
     1381 cggccgtcac gatcggcggc acccgctggt ggtaaggatc atgccgttca gacctcccga
     1441 aaaaacccgc ttgcagtcga ggaagacgag gcttgtcaag tgtttgtcct gaccgaaccc
     1501 ggccgaaccg acccgtattc tgctaccgtt ttaatttgag agcaatacca tttgttcctg
     1561 atactattct gatgatactt ttattaacat acaacgcact cacatggaaa atcgcaccgc
     1621 acgcctcacg atcctcatcg accctcgcaa gaaacaaatc ttcgaggaga tctgcgccgc
     1681 gcatgacctg acaccgtcac aggtggtccg caagttgatt cgccagtacg tcatcgacaa
     1741 cgccggcgac cgtgaactgc cggactggct gcgcgcacgc ccctccgccg accgcgacgg
     1801 ctgagcgacg cccgatgcgc atccggccgg gccggcgctg cttcccggca cggcagccgt
     1861 catccaggcc cgccgacacc gacatatcga agtgctaaga ttcgcgctcg tccagacacc
     1921 gcgccaatca tcaagggaga tgctgcatga aactcgaaac gctcgccgtc cacgccggct
     1981 acagccccga accgacgacc aaggcggtcg ccgtaccgat ctaccagacc tcctcgtacg
     2041 ccttcgacag cacgcaacac ggcgccgacc tgttcgacct caaggtggcc gggaacatct
     2101 atacccggat catgaatccg acccaggatg tgctcgaaaa gcgcgtggcg gcgatggaag
     2161 gtggcatcgc tggcctcgct ctggcgtcgg gtcaggcggc gatcacctac gcgatcatga
     2221 ccatctccga agccggcgac aacatcgtct cggcatcgac gctgtatggc ggcacctaca
     2281 acctgttcgc gcacaccctg ccgcagtacg gcatcgaagt ccgcttcgcc aaccacgccg
     2341 atccggccag tttcgaaaga ctgatcaacg gcaagaccaa agcgctgtac tgtgagtcgg
     2401 tcggcaaccc gctcggcaac atcaccgact tcgagcaact cgccgagatt gcgcaccgcc
     2461 acggcgtgcc gctgatcgtc gacaacaccg ttccctcgcc gtatttgtgc cgccccttcg
     2521 agcatggcgc ggacatcgtc gtacactcgc tgaccaagta tatcggcggc catggcaact
     2581 cgatcggcgg catgatcgtc gacagcggcc gcttcccgtg ggccgagcac aagctgaaat
     2641 tcaagcgcct caacgagccc gacgtctcct accacggcgt cgtctatacc gaagccctgg
     2701 gcccggcggc ctatatcggg cgcgcgcgcg tggtgccgct gcgcaacatg ggcgccgcca
     2761 tctcgccact gaacagcttc ctgattctgc agggcctgga gacgctgccg ctgcgcatgg
     2821 atcgcatctg cgccaacacc gaaaggatcg ccgcttacct gcagggccac gccaaggtgg
     2881 catgggtcaa ttacgccggt ttgcccgatc atccggacca tgcgctggta cagaagtaca
     2941 tggacggcaa ggcttccggg atcctcacct ttggcgtcaa gggtaccgac tgcagcggca
     3001 ccgaggcggg cgcgcgcttc caggacgcat tgcagttgtt cacccggctg gtcaacatcg
     3061 gcgacgccaa gtcgctcgcc tgccatccgg catcgaccac gcatcgccag ttgggagcgg
     3121 ccgaaatggc caaggcaggc gtctccgaag agatgattcg cctctccgtc ggtatcgagc
     3181 atatcgacga tctggtcgcc gatctggaac aggcgctggc cgccgcgtga gcggcgctgc
     3241 tctggattcg tccggtgcag caacgcaccc ggggcgtcgc ggcgagcgca cggccaattt
     3301 ttgggttagg atgcgattat cgtcctgtgc ggatcgtatc agtcgatgtc caccagccca
     3361 taccgcctgc cgctcgtcga cttgcgtgcc gatgatgcgg aaatcggaaa ctacgccgcg
     3421 ctgctcgaca gcctgaacat cggcttgctg gtgttcgacg ccaatggcgc gttgcgcctg
     3481 cgcaatgcgc acgccgagca cctgcttggc gccggtccag gcatctggga agatgaaaac
     3541 ggccagccgc tggtcgccga cgaccgcctc gaaaagcagg tcgtgcacac ccggcagccg
     3601 gttcgtcaac gggccttcgg catccggcgt gatcctgcgg gtacgcgcac ctggtgcaaa
     3661 gccagcgctt ttcccgtgtt ttccgggaaa ggcagcctgc gccgcgttct cctgaccctg
     3721 gccgacctca gccagtacag ccggctggca accgaaaacc cgccgctgcc gacgcacgac
     3781 ccgctcaccg gagttttcag cgagcgttac atctcactcc tgctcgacga cgaagatcgc
     3841 cgggctcggc gctacggcac gccgtttgcg ttggccctgg tgactcccga cgattattcc
     3901 gaattctgcg caagacacgg cgcgccggcg gctgcaagcc tgctggccga tgtcggccgc
     3961 gtgctcggca ggggcctgcg cgaattcgac atggtgggcc gccttggcga cgaggagttc
     4021 ctgctgattc tgcccaacgt acgcgtcaat gacgccatgc acggcctgga acgactgcgt
     4081 gaaatggtcg aaagcagcca tccgggcgat ggcgagccgg cactgacgat cagcggcggc
     4141 gtcaccgaat gcaccggcga ggacgctgcg gtgcttatcg agcgtgtccg ctcattgctc
     4201 gccagcgcgc gcgaagccgg tggcaaccgg ctgtgcgtcg atccggagat tttttgagcg
     4261 gcgggcggcg cgtcccaccg ctcacgacag atgcggcaaa ccgagatact cgcgcgaacg
     4321 catctcggtc agccgactgg ccgtgcgaaa gaactcgctg gcctgcgtgc cctgcatata
     4381 caggctgtcc ggaccgcaat cggcagtggc aatcagcttg accttgtgat cgtagaaaat
     4441 gtcgaccagc caggtgaaac gccgggcttc cgatgacatg ctcgccgaca tccggggaat
     4501 cgccgagagc agcaccgtgt gatagccgcg cgcgagttcg aggtagtcgt tctgcgagcg
     4561 tggtccgccg cacagagttc ggaagtcgaa ccag
//
LOCUS       DAOSIT010000137         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16653_length_4585_cov_10.045033, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000137 DAOSIT010000000
VERSION     DAOSIT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16653_length_4585_cov_10.045033"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2124
                     /gene="aceE"
                     /locus_tag="PLL14_04630"
     CDS             <1..2124
                     /gene="aceE"
                     /locus_tag="PLL14_04630"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOG03239.1"
                     /translation="DFWQFPTVSMGLGPLQAIYSARFMKYLDSRGFIEARDRKVWAFL
                     GDGETDEVESLGAIGMAARERLDNLIFVINCNLQRLDGPVRGNGKIIQELEGTFRGAG
                     WNVIKLIWGTNWDTLFLRDKKGILKQRMMAALDGEYQTFKAKDGAYVREHFFNTPELK
                     ALVADWTDDQVWALNRGGHDIFKIFSAYKSAVEHQGQPTLILAKTIKGFGMGQAGEAM
                     NISHQQKKLDYEAIKRFRDRFNLPVRDDQVEELPYLKFAEGSPELTYMRQRRMDLGGY
                     LPKRRRTAPALEVPALAAFDALLKASGEGREVSTTMAIVRFLNILLKDKKVGRHVVPI
                     VPDESRTFGMEGLFRQIGIWNQEGQKYVPEDHDQLMFYKESKDGQVLQEGINEAGAMS
                     DWIAAATAYSVHGVQMIPFYICYSMFGLQRTMDLCWAAADQRSRGFLIGGTAGRTTLN
                     GEGLQHEDGHSLVLSSLIPNCLSYDPTFSFEIAVIMQEGMRRMYQEQEDIFYYVTVMN
                     ENYEHPEMPVGAAADILKGMYLLRRGHSSDTNPAAPRVQLLGSGTIFREVIAAAELLK
                     NDWGVDADLWGCPSFTELARNGSDVQRWNMLNPIAKPKLSHVESCLGDTRGPIVAATD
                     YVRMYAEQIRPFLNRRYVTLGTDGFGRSDTRAKLRHFFEVDRRWVTVAALKALADDGT
                     IERGKVAEAITRYGIDVNKPNPTTV"
     gene            2137..3759
                     /gene="aceF"
                     /locus_tag="PLL14_04635"
     CDS             2137..3759
                     /gene="aceF"
                     /locus_tag="PLL14_04635"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HOG03240.1"
                     /translation="MSQLIEVTVPDIGDYTDVPVIDICVIVGDVVKVDDALVTLESDK
                     ATMDVPSSAAGVVREIRVALGERISAGAVVVVLEASEQAAATPVAAAPLAAPVAAPAP
                     AVAAAVPVAVPAGGAVEVKVPDIGDYHDVPVIDVCVKVGDTVKVDDALVTLESDKATM
                     DVPSSVAGVVREIRVKLGDKVSEGTVVVVVDRGGDSAVVASVPAAPAPVAAVSVVASD
                     PVPAVPASPPGVGLAPGARTHASPSVRLLARELGVDLNKVTASGPKGRILKEDVTAYV
                     KGVMTAVPAVAVAAAPPLGGGLDLLPWPKVDFAKFGPVEIKPLARIKKISAQNLARNW
                     VMIPAVTYHEDADITDLEAFRVAMNKENEKSGLKITMLAFLIKACTVALKKFPEFNSS
                     LDGDNLVLKQYCHVAFAADTPNGLVVPVIKNADQKSVAQLAIESGELARKARDGKLSP
                     AEMSGACFTISSLGGIGGTYFSPIVNAPEVAILGVNKSVMKPVWNGAAFAPRLILPLS
                     LSADHRVIDGALATRFNVFIAQLLADMRRVLL"
     gene            3776..>4585
                     /locus_tag="PLL14_04640"
     CDS             3776..>4585
                     /locus_tag="PLL14_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG03241.1"
                     /translation="MSQIIEAIVPDIGDYHDVPVIDVCVQAGDTVRIDDALITLESDK
                     ATMDVPSSVAGVVREVKVKLGDKLSEGTVVALIESGGTAEATATPVAAAAGAPPASAA
                     LVPQAAAPITGSADLECEMLVLGAGPGGYSAAFRSADLGMQTVLVERYATLGGVCLNV
                     GCIPSKALLHIAGVMEEAQHMGDCGVSFAAPSIDLDKLRAHKDKVVGKLTGGLAGMAK
                     GRKVQVVQGVGQFLDPNHLEVALADGGRKVIRFAKAIIAAGSQPIALPFMPD"
BASE COUNT          857 a         1454 c         1464 g          810 t
ORIGIN      
        1 gatttctggc agtttccgac ggtatcgatg ggtctcggcc cgctgcaggc catctacagc
       61 gcccgcttca tgaaatacct cgacagccgg ggtttcattg aagcccggga ccgcaaggtc
      121 tgggccttcc ttggcgacgg cgagacggac gaagtcgaat ccctgggtgc catcggcatg
      181 gcggcgcgcg agcggctcga taatctgatc ttcgtgatca actgcaatct gcagcgcctc
      241 gatggccctg tgcgtggcaa cggcaagatc attcaggaac tcgaaggcac ttttcgcggt
      301 gccggctgga acgtcatcaa gctgatctgg ggaaccaact gggacacgct tttcctgcgc
      361 gacaagaaag gcatcctcaa gcagcggatg atggctgccc tcgacggcga ataccagacc
      421 ttcaaggcca aggacggggc ctacgtgcgc gagcacttct tcaacacgcc ggaactcaag
      481 gcgctcgtcg ccgactggac cgacgaccag gtctgggcgc tgaaccgtgg cggccacgac
      541 atcttcaaga ttttcagcgc ctacaagagc gcggtcgagc atcaaggcca gccgaccctg
      601 atcctggcca agaccatcaa gggcttcggc atgggccagg ccggcgaagc gatgaacatc
      661 tcgcatcagc aaaagaaact cgattacgaa gcgatcaagc gtttccgtga ccgtttcaac
      721 ctgccggtgc gcgacgacca ggtcgaggaa ctgccctacc tcaagttcgc cgaaggctcg
      781 cccgaactca cctacatgcg ccagcggcga atggacctcg gcggttacct gccgaagcgt
      841 cgacgtaccg cgccggcgct ggaggtgccg gcactggccg ccttcgatgc gctgctcaag
      901 gcctcgggcg aggggcgcga ggtgtccacg accatggcca tcgtccgttt cctcaacatc
      961 ctgctcaagg acaagaaggt cggccgtcac gtcgtcccga tcgttcccga tgaatcgcgc
     1021 accttcggga tggagggtct gttccgccag atcggcatct ggaatcagga aggccagaag
     1081 tacgttccgg aagaccacga ccagttgatg ttctacaagg agtcgaagga cggccaggtt
     1141 ctccaggaag ggatcaacga ggccggtgcg atgagcgact ggatcgccgc ggcgacggcg
     1201 tattcggtgc atggcgtgca gatgatcccg ttctacatct gctactcgat gttcggcctg
     1261 caacgcacga tggacctttg ctgggcggcc gccgatcagc gctcgcgtgg cttcctgatc
     1321 ggcggcaccg ccggccggac gacgctgaac ggcgaaggcc tgcagcacga agacggccat
     1381 tcgctggtgc tctccagtct gatcccgaac tgcctgagct acgacccgac cttctccttc
     1441 gaaatcgccg tgatcatgca ggaaggcatg cgccggatgt atcaggaaca ggaggacatc
     1501 ttctattacg tcaccgtgat gaacgagaac tacgagcatc cggaaatgcc ggtcggcgct
     1561 gcggccgaca tcctcaaggg gatgtacctg ttgcgccgcg gccattcgtc ggacaccaat
     1621 ccggctgcgc cgcgcgtaca actgctcggt tccgggacga tcttccgtga agtgatcgcc
     1681 gctgccgaat tgctcaagaa cgactggggc gtcgatgccg acctctgggg ttgcccgagc
     1741 ttcaccgaac tggcgcgcaa cggcagcgac gtccagcgct ggaacatgct caacccgatt
     1801 gccaagccga agctgtcgca cgttgaaagc tgcctcggcg acacgcgcgg cccgatcgtt
     1861 gccgcgaccg actacgtgcg catgtacgcc gagcagatcc gtcctttcct caaccgtcgc
     1921 tacgtgacgc tgggcaccga cggcttcggt cgctcggaca cgcgcgcaaa gttgcgccat
     1981 ttcttcgagg tcgatcgccg ctgggtcacc gtggccgccc tgaaggctct cgccgacgac
     2041 ggcacgatcg agcgcggcaa ggtcgccgag gcgatcaccc gttacggcat cgacgtgaac
     2101 aaacccaacc cgacgacggt ttaagggagc cctctgatga gtcaattgat cgaagtaaca
     2161 gtccccgaca ttggtgatta caccgacgtg ccggtgatcg acatctgcgt catcgtcggc
     2221 gacgtcgtca aggtcgatga tgcgctggtg accctggaat cggacaaggc gacgatggat
     2281 gtgccgtctt cggcagccgg ggtggtcagg gaaatccgcg tcgcactcgg cgaaaggatt
     2341 tccgcaggcg ccgtcgtcgt cgtcctcgaa gcatccgagc aggctgccgc gacgccggtc
     2401 gccgccgccc cccttgccgc cccagttgcg gcgccagcgc cggctgttgc cgcggcagtt
     2461 ccagtggcag tccctgctgg cggagcggtc gaggtcaagg tgcccgacat tggtgactat
     2521 cacgatgttc cggtgatcga cgtgtgtgtc aaggttggcg acacggtcaa ggtcgatgac
     2581 gcgttggtca cgctcgaatc cgacaaggca acgatggatg tgccctcgtc ggtggccggg
     2641 gtggttcgcg agatcagggt caagctcggc gacaaggttt ccgagggcac ggtggtggtg
     2701 gtcgttgacc gcggtggtga ctctgctgtc gttgcttctg ttcctgctgc tcccgcgccg
     2761 gtcgcggcgg tatcggtggt cgccagtgat ccggtgccag cggtaccagc ctcgcctcca
     2821 ggtgttggcc tggccccggg cgcccggacg catgccagcc cgtcggtgcg cctgctggcc
     2881 cgcgaactcg gcgtcgatct caacaaggtc acggccagcg gacccaaagg ccgcattctc
     2941 aaggaagacg tcacggctta cgtcaagggg gtcatgaccg ccgtcccggc ggtcgccgtg
     3001 gctgccgcac ccccgctcgg cggcggtctc gacctgctgc cctggccgaa ggtcgacttc
     3061 gccaaattcg gccccgtcga aatcaagccg ctggcgcgga tcaagaagat ctccgcgcag
     3121 aacctggcgc gtaactgggt gatgattccc gcggtcacct atcacgaaga cgccgacatt
     3181 accgacctgg aagccttccg cgtggcgatg aacaaggaga acgagaaatc gggactcaag
     3241 atcaccatgc tggctttcct gatcaaggcc tgcacggtgg ccctgaagaa gttccccgag
     3301 ttcaacagtt cgctggatgg cgacaacctg gtgctcaagc agtattgcca cgtcgccttt
     3361 gcggccgaca cgccaaacgg tctggtggtg ccggtgatca agaacgccga ccagaaatcg
     3421 gtggcccaac tggccatcga aagtggtgaa ctggccagga aggcgcgcga cggcaagctt
     3481 tcgccggccg agatgtccgg ggcctgcttc accatttcca gcctcggcgg catcggcggc
     3541 acgtacttct cgccgatcgt caatgcgccc gaagtggcca tcctcggggt caacaagtcg
     3601 gtcatgaagc cggtctggaa cggcgcggcg ttcgcgccgc gtctgatcct gccgttgtcg
     3661 ctgtccgccg accaccgcgt catcgacggc gcgctggcga cccgcttcaa cgtcttcatc
     3721 gcgcaactcc ttgccgacat gcggcgcgtg ctgctctgat cgggagaaga aaatcatgag
     3781 ccagatcatt gaagcaatag tgcccgacat cggcgactat cacgatgtcc cggtcatcga
     3841 cgtgtgtgtc caggccggcg acacggtcag gatcgatgac gccctgatca ccctcgaatc
     3901 cgacaaggcg acgatggacg tcccgagcag cgtcgccggc gtggtcaggg aagtgaaggt
     3961 caaactcggc gacaaactct ccgagggtac ggtggtggcc ctgatcgaga gcggtgggac
     4021 tgccgaggca accgccacgc cagtggccgc cgctgccggc gcgccgccgg caagcgctgc
     4081 cctggtgccg caggccgcgg cgccgatcac cggcagtgcc gacctcgaat gcgagatgct
     4141 ggttctcggc gccggaccgg ggggctactc ggcggctttc cgcagcgccg acctggggat
     4201 gcagacggtg ctcgtcgagc gttacgcgac gctcggtggc gtctgcctga acgtcggctg
     4261 catcccttcg aaagcactgt tgcacatcgc cggcgtaatg gaagaagcgc agcacatggg
     4321 cgattgcggc gtcagcttcg ctgctccgag catcgatctc gacaagctgc gtgcccacaa
     4381 ggacaaggtc gtcggcaaac tgaccggcgg tcttgccggc atggccaagg ggcgcaaggt
     4441 ccaggtcgtg cagggtgtcg gccagtttct ggacccgaac catctcgaag tcgcgctagc
     4501 ggacggtggc cggaaagtca tcaggttcgc gaaggcgatc atcgccgccg gttcgcaacc
     4561 gatcgcgctg ccgttcatgc ccgac
//
LOCUS       DAOSIT010000138         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16702_length_4573_cov_9.302789, whole genome shotgun sequence.
ACCESSION   DAOSIT010000138 DAOSIT010000000
VERSION     DAOSIT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16702_length_4573_cov_9.302789"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PLL14_04645"
     CDS             complement(<1..1409)
                     /locus_tag="PLL14_04645"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOG03242.1"
                     /translation="MLDLLLAGLGALLALAFTGALVYLYVQDVTQEKHAVLRNFPLVG
                     HLRYFFESLGEYFRQYFFLGDREEMPFDRATRSWVYRMAKNEGGIIGFGSTYRIHEAG
                     ALIFVNAPFPVLEEEQLPTPAVVVGEGYCRTPFIGRSLVNISAMSFGAISRPAVRALS
                     RGAAAAGCWLDTGEGGLSPYHLESSCDIIFQIGTAKYGVRDEHGELSEVRLRELAANA
                     TVRAFEIKLSQGAKPGKGGVLPGNKVTPEIAGIRGIAEGCDSMSPNRHRDIANIDELL
                     DKVEQVREVTGKPVGIKTAIGGDRFMLELTAAVARRGLQAAPDFLTIDGGEGGSGAAP
                     QALADHMALAIDEALPLIVDALLASGLKERIRIIAAGQLVTPARVAWALSAGADFVNT
                     ARGFMFSLGCIQALRCHTNTCPTGVTTHDPRLQRGLVVADKAARVASYCRNMNKEVAM
                     IAHSCGLRHARELRREHVR"
     gene            1469..>2184
                     /gene="nadC"
                     /locus_tag="PLL14_04650"
     CDS             1469..>2184
                     /gene="nadC"
                     /locus_tag="PLL14_04650"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOG03243.1"
                     /translation="MTLAALLDAEIERNVTAALTEDVGSGDLTAQLVPAGEFSRATVI
                     AREDAVLCGSAWFERCFRKLDAAVAITWKAEDGQRLQPNQLLCEIEGPARSLLTGERT
                     ALNFLQLLSGVATMARQYADRVAGTGAQVVDTRKTIPGLRLAQKYAVRCGGGGNHRLG
                     LYDGILIKENHILAARSIPAALAAAMQLAERTAGVCQFVQIEVETLAELKQALDAGAV
                     MILLDNMSLEQLREAVAIAGG"
     assembly_gap    2185..2284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2285..3633)
                     /gene="thrC"
                     /locus_tag="PLL14_04655"
     CDS             complement(<2285..3633)
                     /gene="thrC"
                     /locus_tag="PLL14_04655"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG03244.1"
                     /translation="MRYISTRGHLSGMSTSSGPTFSEILLGGLAPDGGLYLPESYPQV
                     SSSELEQWRQLSYADLAWVVLSRFVDDIPPADLKALVARTYTAEVYGNGRRKEAAGEI
                     TPLLWLERPDPARAEGGLAILELSNGPTLAFKDMAMQLLGHLFEYVLARQGAEINILG
                     ATSGDTGSSAEYAMRGKRGVRVFMLSPHDRMSAFQRAQMYSLHDANIHNIAVRGTFDD
                     AQDIVKAVSNDHAFKAKFKIGAVNSINWARVAAQVVYYFKGYFAATAAAPRGSNQQVA
                     FAVPSGNFGNICAGHIARMMGLPIGRLVLATNENDVLDEFFRTGVYRPRSTAETCATS
                     SPSMDISKASNFERFVFDLVGRDASVVRKLWAEVDAGRSFDLSGTPFLARLPQFGFIS
                     GSSTHADRLATIRQTFAKYGLMIDTHTADGLKVGSELRTPDMPMVVLETALPAKFEQT
                     "
     gene            complement(3736..4365)
                     /locus_tag="PLL14_04660"
     CDS             complement(3736..4365)
                     /locus_tag="PLL14_04660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG03245.1"
                     /translation="MLLPTANGRTPHTHVRRIDAPWPQATVLELMAAELRIGELETAL
                     CEARAAAFTDTLTGALNRRGFDDAHRRELARARRSGSSMALALIDIDDFKRLNDTLGH
                     HVGDQALVHLVKVLRAALRPSDILCRFGGEEFVLLLPETELNAAAAAISRFQRQLAAH
                     AIPGTGAVLTFSAGVVMQEVGEALDHTILRADAATYAAKRAGKNCVITG"
BASE COUNT          875 a         1338 c         1420 g          840 t
ORIGIN      
        1 atgcgtacgt gttcgcggcg gagttcgcgg gcatggcgca gaccacagga gtgagcgatc
       61 atcgcgacct ccttgttcat gttgcggcag tatgacgcta ctcgcgcggc cttgtccgca
      121 acgaccaggc cgcgttgcag acgcgggtcg tgtgtggtga cgccggttgg acaggtattg
      181 gtatggcagc gcaaggcctg gatgcagccc agcgagaaca tgaagccgcg ggcggtgttc
      241 acgaaatcgg ctcccgccga caatgcccag gcgacacgcg ccggggtcac caactgaccg
      301 gcggcaatga tccgaatacg ctccttgagt ccgctggcca gcagcgcgtc gacgatcagc
      361 ggcagcgctt cgtcgatcgc cagcgccata tgatcggcca gggcctgcgg tgcggcacca
      421 ctgccccctt cgccaccatc gatggtgaga aagtcgggcg ccgcctgcag accacgccgt
      481 gcgaccgccg cagtgagttc aagcatgaag cgatcaccgc cgatggcagt cttgatgccc
      541 accggtttgc cggttacttc ccgaacctgt tcgaccttgt ccagcaattc gtcgatgttg
      601 gcgatatccc ggtgccggtt cgggctcatc gaatcgcaac cctcggcaat gccgcgaatg
      661 ccggcgatct cgggcgtgac cttgttgcca ggcagaacgc cgcccttgcc cggcttggcc
      721 ccctgcgaaa gcttgatctc gaaggcccgc accgttgcgt tcgccgccag ttcgcgcagg
      781 cggacctccg acagttcgcc atgctcgtca cggacgccgt atttcgcggt cccgatctga
      841 aaaatgatgt cgcaactact ctccagatga taaggagaaa gtccgccctc gccggtatca
      901 agccagcagc cagccgcggc tgcaccgcgg gataacgcgc gtacggctgg tcgggaaatg
      961 gcgccgaagc tcatcgcgct gatgttcacc agtgatctgc cgatgaaggg cgtccggcaa
     1021 taaccttcac cgaccactac cgcaggtgtc ggcagttgct cctcctcaag cacaggaaag
     1081 ggagcgttga cgaaaatcag ggcaccggct tcgtgaatcc gataggttga tccaaagccg
     1141 ataatcccgc cctcgttttt cgccatgcga tagacccaac tgcgcgtcgc ccggtcgaag
     1201 ggcatttcct cacggtcgcc aaggaagaag tattggcgga agtattcgcc gaggctctcg
     1261 aaaaagtaac gcaggtgacc gaccagcgga aagttccgca gtacggcatg tttttcctgc
     1321 gtgacgtcct gcacgtagag gtagaccagg gcgccggtga aggccagggc aagcagtgcg
     1381 cccagtccgg caagcagtag gtcaagcatt tgccgctccc atcggtgtta gaattgagca
     1441 agtcacctct tagtgtacgg gatttgccat gacccttgca gccctgcttg atgccgaaat
     1501 cgagcgcaat gtcaccgccg cactcacaga ggatgtcggg agcggtgacc tgaccgcgca
     1561 actcgtcccg gctggcgagt tcagtcgcgc cacggtgatt gcccgtgagg acgcggtact
     1621 ctgtgggagt gcctggttcg agcgttgctt cagaaagctg gatgcggcgg tcgccattac
     1681 ctggaaggca gaagatggcc agcggttgca gcccaatcag ttgctttgcg agatcgaggg
     1741 tcctgcgcgc tccctgctca ccggcgagcg aacagcgctc aatttcctgc aactgctgtc
     1801 cggcgttgcc accatggccc gccagtatgc ggatcgggtg gccggtaccg gggcacaggt
     1861 ggtcgatacc cggaagacga tccctggctt gcgtctggcg caaaaatacg cagtcaggtg
     1921 cggcggcggt ggcaatcacc gcctcggtct ttatgacgga atcctgatca aggaaaacca
     1981 cattctcgcc gcccgcagca ttccggcagc gctcgccgct gccatgcagc ttgccgagcg
     2041 tacggccgga gtctgccagt tcgtgcagat cgaggtcgag accctcgccg agttgaagca
     2101 ggccctcgat gccggtgcgg taatgatcct gcttgacaat atgagcctgg agcaactgcg
     2161 tgaagccgtg gccatcgccg gtggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnngtttgc tcgaacttgg ccggcagggc cgtttcgaga accaccatcg gcatgtcggg
     2341 cgtgcgtaac tcgctgccga ccttgagccc gtcggcggtg tgcgtatcga tcatcaggcc
     2401 gtacttggcg aaggtctgac ggatcgttgc cagccgatcg gcatgtgtgc tgcttccgga
     2461 aatgaagccg aattgaggca ggcgggcgag gaagggcgtg cccgagaggt caaaactgcg
     2521 acctgcatcc acctctgccc atagcttgcg caccacgctc gcatcacgtc ctaccaaatc
     2581 gaagacaaat cgttcgaagt tggatgcttt cgagatgtcc atcgaagggc tcgacgtggc
     2641 gcaggtttcg gcggtcgagc gtggccggta gacgccggtg cggaaaaatt cgtcgagtac
     2701 gtcgttctcg ttggtcgcca gcaccagtcg cccgatcggc aaacccatca tgcgcgcgat
     2761 gtggccggcg cagatgttgc cgaagttgcc cgaaggaacg gcaaaggcca cctgttgatt
     2821 gctgccacgg ggtgccgcgg cagtagcggc gaagtaaccc ttgaagtagt acaccacctg
     2881 cgcagcgacg cgcgcccagt tgatcgagtt gaccgctccg atcttgaact ttgccttgaa
     2941 ggcatgatca ttggacaccg ccttgacgat gtcctgggca tcgtcaaagg tgccgcgtac
     3001 ggcgatgttg tggatattgg cgtcgtgcag cgaatacatc tgcgcacgct ggaaagcgct
     3061 catccgatca tgcggcgaaa gcatgaacac ccgcacaccc cgcttgccgc gcatcgcata
     3121 ttcggcagac gagccggtat ctcccgaggt ggcgccaaga atgttgatct ctgcgccttg
     3181 tcttgccagg acgtattcga aaagatggcc aagcaactgc atggccatgt ccttgaaggc
     3241 aagcgtcggg ccgttcgaga gttcgaggat ggccaggcca ccttctgcgc gcgccgggtc
     3301 cggtcgctcc agccagagca agggagtgat ctcgcctgca gcttccttgc ggcggccgtt
     3361 gccatacacc tccgcggtgt aggtcctggc caccagggct ttcagatctg ccggcgggat
     3421 gtcgtcaaca aatctggaga gcacgaccca ggccaggtcg gcatacgata gttgccgcca
     3481 ttgctcgagt tcgctgctgc tgacttgcgg ataggattca ggaagataga gaccaccgtc
     3541 cggagccaga ccgccaagca ggatctcgct gaaggttggg cctgaggaag tggacatacc
     3601 ggaaagatga ccgcgagtcg agatatagcg catggtgatg accgtgaggg cgaaagcgga
     3661 gggatgacgg caagcgagca gtctaccacg ctctcaccct ggtgctggag ggccggcggc
     3721 ttccaggccg aaagctcagc cagtgatcac gcaattcttg cccgcgcgtt tggccgcata
     3781 ggttgccgca tccgcacgca gaatcgtgtg gtccagggct tcgccgactt cctgcatgac
     3841 gacgcctgcg ctgaaagtca ggacggcacc cgtgcccggg atggcgtgtg ctgccagttg
     3901 ccgctggaag cgcgagattg ccgccgccgc cgcgttcagt tcggtctccg gcagcagcag
     3961 gacaaactcc tcgcctccga agcggcaaag aatgtcggac gggcgcagag cagcgcgcag
     4021 gaccttgacc aggtgcacca gggcctggtc gcccacgtgg tgtccaaggg tgtcgttcaa
     4081 acgcttgaaa tcatcgatat cgatcagtgc cagggccatc gagctgccac ttctgcgtgc
     4141 gcgcgccagt tcgcgccggt gagcatcatc aaaaccacgg cggttgaggg cgcccgtcag
     4201 ggtatcggtg aaagcggccg cacgcgcttc gcacaaggct gtttcgagtt ctccgatacg
     4261 cagttccgcg gccatcagtt ccagcaccgt tgcttgcggc caaggggcat caatgcggcg
     4321 aacgtgggtg tgcggcgtac ggccgttggc tgtgggcagc agcatctgcg gttgtgctga
     4381 tgagagggtc atggctggcg ggcgcggatg cagatgacga gacgcgcagc gcgaggaatg
     4441 aacaggggca gagtggccat gcttgaacat attcttgctt ctccgtcagg ggtgcagttc
     4501 agggttggac atactcccag caacggacgc ccgccggaga tcttgagact ttattcacga
     4561 aaatcattca aca
//
LOCUS       DAOSIT010000139         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16718_length_4569_cov_8.691848, whole genome shotgun sequence.
ACCESSION   DAOSIT010000139 DAOSIT010000000
VERSION     DAOSIT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16718_length_4569_cov_8.691848"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLL14_04665"
     CDS             complement(<1..1076)
                     /locus_tag="PLL14_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG03246.1"
                     /translation="MSPARRFGFEWLLLSAALLFLGGATAWWSLWLPYQRLEQEENDR
                     LQAIAMTLGDAIGHRLGAAEHALAAVAGDVGYWRGGAERVSRLQALARVLDGIHTLLI
                     LDAAGRVVAASRADLIGQDFGARKFFIGAREQPGARWLELDAEAENGHTPYRLGVSRA
                     IIDRRGGFAGVVAAVLDGDFIGSLMESGRYAPDVRLLLVHADGRPYVAAPHNAQAWDV
                     TAAASLFALHRESGQRVSSMNRANPARGEDLVVAVHALHADSSAADADLLLAVARERA
                     LVHAQWRREEAWHGGLFLATLLSSVIALQAVQQRRRRLDALRAERTAELRAATERLRL
                     VADTAGLGIWEYEPAGRRVHWDAS"
     gene            1335..1946
                     /locus_tag="PLL14_04670"
     CDS             1335..1946
                     /locus_tag="PLL14_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03247.1"
                     /translation="MALPTNQSVCIVDDDVQVRRFLVEVLSSIGLAAEEYPSGEDFMR
                     RWRAGNTGCVLLDIRMPRVTGPEVHDWLRQRNPEVPVIFLSGYADVPTAVRAMRLGAF
                     DFLEKPFNVQQLLERVNAALQLADERRNATTGLASEDDWRQALTPREQEILGAIVDGK
                     RNKVIAADLGISERTVETHRAHIMAKAGAGSVIELVGMVIGRK"
     gene            2151..2519
                     /locus_tag="PLL14_04675"
     CDS             2151..2519
                     /locus_tag="PLL14_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03248.1"
                     /translation="MAKTIFLVDDSATILLSVSSILGKAGFAVEKASNADEALRKFKA
                     GVKVDLLITDLNMPGMNGIELIKQVRTLAAYRFLPILFLTTESQQSRKLEAKAAGASG
                     WIVKPATADDLLNTIKLVIR"
     gene            2529..3887
                     /locus_tag="PLL14_04680"
     CDS             2529..3887
                     /locus_tag="PLL14_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03249.1"
                     /translation="MTTAPRQPYQQYRQRTLITGIAAAIVLVIVIFLVHDFFDEFLES
                     RFGLGDRSADTLITLFGLLLFVAIQHAVSRILYHDVHMGIETGLKDVRQRCPSNKVCQ
                     RVAMPELREIPRFNKVLVGQLRSVVEQTEKAAYDVTSRLQTIDEVVTDLNHFVAGAAA
                     ESETMALDSEAKIGGNRQLISKLEAFIAQRIAETQQDQERSAEAVREAKSLQTLVDLI
                     KHIAGQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLSHETENAVKKINDGIIAVAN
                     IIETQFRDKLAHSHINEERDSLECFAQQLSSLGASYESLTARERTILETITASSGKLG
                     AMFMDTLASVQFQDVTRQQIEHVISGIERVDSHASSLAGVLERGEDVRREDAVEPLAA
                     QIEQVYSTYVMAQQRDAHTQAMANSNAAPRGPAVTKAKGRAAAHAVPPAAPKKPSNVE
                     LF"
     gene            3920..4225
                     /locus_tag="PLL14_04685"
     CDS             3920..4225
                     /locus_tag="PLL14_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG03250.1"
                     /translation="MNASSANRLAIREDMTIYQAQAQKEQLLAALAATEDLELDLSGV
                     GEMDTAGLQLLLLLKREVLQQGKQLKIISHSPSVQHLFDFCNLVGVFGDPMLISAKP"
     gene            4250..>4569
                     /locus_tag="PLL14_04690"
     CDS             4250..>4569
                     /locus_tag="PLL14_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG03251.1"
                     /translation="MDELTSTFVNESRDQLIAMEDGLLRLEQNPDDADNLGAIFRAAH
                     TIKGGSGVVECHFIEAFTHKVENVLDKLRNGEIPVSGELSTVMLECCDHLGALLDVLA
                     AGSP"
BASE COUNT          931 a         1557 c         1339 g          742 t
ORIGIN      
        1 atcgacgcat cccaatgcac gcgcctaccg gcgggttcgt actcccagat gcccaatcct
       61 gccgtgtcgg ccaccaggcg aagacgctcg gtagccgccc gcagctcggc ggtgcgctca
      121 gcgcgcaggg cgtcgaggcg acgccggcgt tgctgtacag cctgcagggc aatgaccgac
      181 gacaacagcg tggccagaaa caaaccgcca tgccaggcct cctcgcggcg ccactgggca
      241 tgcaccagcg cccgctctct ggcaacggcc agcaggagat cggcatcggc tgcgctgctg
      301 tcggcgtgca acgcatgcac cgcgaccacc aggtcttcgc cacgtgcagg atttgcgcga
      361 ttcatcgacg aaacccgttg cccactttca cgatgcaggg cgaacaggga tgccgctgcg
      421 gtgacgtccc atgcctgggc attgtggggt gccgccacat acggcctgcc gtcagcgtgg
      481 accagcaaca gccggacgtc cggcgcgtag cgcccggact ccatcagaga cccgatgaag
      541 tcgccatcca gaactgccgc gacaactccg gcgaagcccc cacgacgatc gatgatcgct
      601 ctcgaaacac ccagacgata cggcgtgtgc ccgttctcgg cctcggcatc aagctccagc
      661 catcgggcac cgggctgctc ccgcgcgcca atgaagaatt ttcgtgcacc aaaatcctgg
      721 ccaatcagat ccgcccggct ggcggcgacc acccgccccg ctgcgtcgag gatcagcaag
      781 gtgtggatac catcgagcac acgggccaac gcctgcaagc gggacacacg ctcagcgccg
      841 ccacgccagt agccgacatc gccggccacg gccgccaaag cgtgctcggc ggcgcccagc
      901 cggtgaccga tggcgtcgcc cagggtcatc gcgatagcct gcaggcgatc gttttcttcc
      961 tgctccaggc gctggtaggg cagccacaga gaccaccagg cggtggcgcc ccccaggaac
     1021 agcagtgcgg cactcagcag cagccactcg aagccgaagc gacgcgctgg cgacaacgac
     1081 gacgccggca tcaggcgagc gccggtttgg cgacgagcgc ttgccggctg gtgctgccag
     1141 gcgccctgga aagagggcag gtactgaatg gctccatcga ctgctccatg gtttcgagca
     1201 cgccggcgta cgctggatga cgttccggcc gtggcatcgc gattttcccg tcattctccg
     1261 tatttttacc gatcaccggt agaatatgct gttgcggatt gtcggcactg gccgaaccgg
     1321 cttgaaggaa gcgtatggcc ttacccacga accagagcgt ttgcattgtc gatgacgacg
     1381 tacaggttcg ccgcttcctg gttgaagtgc tgtccagcat cggcctcgct gccgaggagt
     1441 atccctccgg agaggacttc atgcgccgct ggcgagccgg gaacaccggc tgcgtgctgc
     1501 tcgacatccg catgccgcgc gtcaccgggc ccgaggtgca cgactggttg cggcagcgaa
     1561 atcccgaggt gccggtaatc tttctctccg gctatgccga cgtgcccacg gccgtacgcg
     1621 ccatgcggct cggcgctttc gactttctgg aaaagccgtt caatgtgcaa cagctccttg
     1681 aacgggtcaa cgccgccttg cagcttgccg acgaaagacg taacgccacg accggactcg
     1741 cctccgaaga cgactggcga caggcgctga cgccacgcga acaggaaatc ctcggcgcca
     1801 tcgtcgatgg caaacgcaac aaggtcattg ccgctgacct cggcatcagc gagcgcacag
     1861 tcgaaaccca ccgcgcccac atcatggcca aggccggcgc cggcagcgtc atcgaactgg
     1921 tcgggatggt catcggcagg aaataggcca cccagcgcgc ctcaagccac acccagcccc
     1981 tgttccgcgc accccgcggc cgtccgtcct cagtatttcc acttagagac acgtggatta
     2041 ccggatagcg tcatgccatc cgggccgcta ctctctctcc tgtcagattg tcatctgtgc
     2101 aattagcgtg ccggccggcg cgatgcatgc cgaacaggag aagtctcaac atggcaaaaa
     2161 caatttttct ggtcgatgat tcggcgacca ttctactcag tgtttcgagc attctcggca
     2221 aggcgggatt cgccgtcgaa aaggcctcca atgccgacga ggcgctcagg aagttcaagg
     2281 ccggggtcaa ggtcgatctg ctgatcaccg acctcaacat gcccggcatg aacggcatcg
     2341 aactcatcaa acaggtgcgc acgctggccg cctaccgctt cctgccgatt ctctttctga
     2401 ccaccgaatc gcagcagagc cgcaagctcg aagcgaaagc cgccggcgcc tcgggctgga
     2461 tcgtcaagcc cgccaccgcc gacgatctgc tcaacaccat caagctggtc attcgctagg
     2521 acgaccccat gaccaccgca ccccgccagc cgtaccagca gtatcgccag cgcacgctga
     2581 tcaccggcat cgccgcggca atcgtcctgg tcatcgtgat cttcctggta catgatttct
     2641 tcgacgaatt cctggagtcc cgtttcggac tcggcgaccg cagtgccgac accctgatca
     2701 cgctgttcgg tctgctgctc ttcgtcgcca ttcaacatgc cgtctcgcgc atcctctacc
     2761 atgatgtgca catgggcatc gaaaccggtc tcaaggacgt acgccagcgc tgtccgtcga
     2821 acaaggtctg ccagcgcgtg gcgatgcccg aactgcggga gattccgcgc ttcaacaagg
     2881 tcctcgtcgg gcaattgcgc agcgtcgtcg agcagaccga aaaagcggcc tacgacgtca
     2941 cttcgcgcct gcaaaccatc gacgaggtgg tcaccgacct caatcacttc gttgccggcg
     3001 cggccgccga gtcggaaacg atggccctcg attccgaagc caagatcggc ggcaatcgcc
     3061 agttgatcag caagctcgaa gccttcatcg cccagcgcat tgccgaaacg caacaggacc
     3121 aggaacgtag cgccgaggcg gtgcgcgaag ccaagtcctt gcagaccctg gtcgacctga
     3181 tcaagcatat cgccgggcag accaatctgc tggcgctcaa tgcggcgatc gaggccgcgc
     3241 gtgccggcga ggcggggcgc gggttcgcgg tggtcgccga cgaagtccgc aagctgtcgc
     3301 acgaaaccga aaacgcggtc aagaagatca acgacggcat catcgccgtc gccaacatca
     3361 tcgagacgca gttcagggac aagctggcgc attcgcacat caacgaggag cgcgacagcc
     3421 tcgaatgctt cgcgcagcaa ctctcttccc tcggcgccag ctacgagagc ctcaccgcac
     3481 gcgagcgcac cattctcgaa acgattaccg ccagcagcgg caaactcggc gccatgttca
     3541 tggatacgct ggccagcgtg cagttccagg acgtcacgcg ccagcagatc gaacacgtca
     3601 tcagcggcat cgagcgggtc gacagccacg ccagttctct ggccggagtg ctcgagcgcg
     3661 gcgaggacgt ccgccgcgag gatgccgtcg aaccgctggc cgcgcagatc gagcaggttt
     3721 actcgaccta cgtgatggcg cagcagcgcg acgcgcacac gcaggcgatg gcaaattcaa
     3781 acgccgcgcc gcgcggacca gcagtcacca aggccaaggg gcgagcggca gcccatgccg
     3841 taccaccggc ggcccccaag aagccgtcga acgtcgaact cttttgaccc gccgccacaa
     3901 cccacgcaca ggtgtcagca tgaacgcttc ctccgccaac cgactcgcca ttcgtgagga
     3961 catgaccatt tatcaggcac aggcgcagaa ggaacagttg ctggccgccc tggcggccac
     4021 ggaggacctc gaactcgatc tctcgggagt cggcgaaatg gacaccgccg gcttgcaact
     4081 gctgctgctg ctcaagcgcg aagtcctgca gcagggcaag caattgaaga tcatcagcca
     4141 tagcccgagt gtccagcacc tcttcgactt ctgcaatctg gtcggcgtct tcggcgaccc
     4201 gatgctgatc tccgcgaaac cctgaaccca acgccaacaa ggaagtccga tggacgaact
     4261 gaccagcacc ttcgtcaatg aaagccgcga ccagttgatc gcgatggaag acggcctgct
     4321 gcgactcgaa cagaaccccg acgacgccga caacctcggc gccattttcc gtgccgcgca
     4381 taccatcaag ggcggttccg gggttgtcga gtgccacttc atcgaagcct tcacgcacaa
     4441 ggtcgagaac gttctcgaca agctgcgcaa cggtgagatc ccggtgagtg gcgagctgtc
     4501 gacggtgatg ctcgaatgtt gcgatcacct cggcgccctg ctcgatgtgc tcgccgccgg
     4561 ctcgccgca
//
LOCUS       DAOSIT010000140         4546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16830_length_4546_cov_8.382988, whole genome shotgun sequence.
ACCESSION   DAOSIT010000140 DAOSIT010000000
VERSION     DAOSIT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16830_length_4546_cov_8.382988"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLL14_04695"
     CDS             complement(<1..290)
                     /locus_tag="PLL14_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOG03252.1"
                     /translation="MSATPKLFSEPVELPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     VAMEVGKSILLVAQKSAVKDEPEAEDLYSVGCVANILQMLKLPDGTVKVLVEG"
     gene            complement(376..1647)
                     /gene="clpX"
                     /locus_tag="PLL14_04700"
     CDS             complement(376..1647)
                     /gene="clpX"
                     /locus_tag="PLL14_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG03253.1"
                     /translation="MSEKKSGTEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIELCN
                     DIVRDEIAGDLGAKGAKGDLPTPREISALLDQYVIGQDQAKRILSVAVYNHYKRLRHF
                     GKSSDEIELAKSNILLIGPTGSGKTLLAQTLARMLDVPFVMADATTLTEAGYVGEDVE
                     NIIQKLLQKADYDTEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEG
                     TVASVPPQGGRKHPNQDFVQVDTTNILFICGGAFDGLEKVIRHRSERGGIGFGAEVKS
                     KENKKAIGDVLRSVEPEDLIKFGLIPELIGRLPVLATLEELSTAALVQILVEPKNALV
                     KQYQKLFAMEGVALEIRPAAMMAIARKALERKTGARGLRSIIESVLLDTMYELPGMEN
                     VSEVVIDESVIVGDARPILIYADQPKVSGGN"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..3004)
                     /gene="tig"
                     /locus_tag="PLL14_04705"
     CDS             complement(<1899..3004)
                     /gene="tig"
                     /locus_tag="PLL14_04705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG03254.1"
                     /translation="METNVANTDPSTIVAAVSALERRVDLSVAIAELDREVDQRLQRL
                     GRNMKMPGFRPGKVPIHIVRQQYGEQARHEALTEALGQKLSDEVTARQLRVAGSPKIE
                     PRDSGSTTHIEFSAVFEVYPEITLGDLTGVEISRPVLEVGDAEIDGTIDILRKQRVRY
                     ELVDRPAANADRVSVDFLGKKGGEPFAGGEGKDYRFVLGEGKMLSDFENAVIGTRPGE
                     SKSFELTFPAEYFSKELAGQTVTFELTVKEVGEAILPEVDADFAKALGVADGDLDKMR
                     AEIEDNLRREVKKRLQAKIMEQVMDALLRVNAIEVPGALIHREIERLMQLARQDMEQR
                     GMKVKDLPMQPEWFVDQARRRVTLGLIFAEVVKS"
     gene            3483..>4546
                     /locus_tag="PLL14_04710"
     CDS             3483..>4546
                     /locus_tag="PLL14_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOG03255.1"
                     /translation="MKITSSEMQMVSSHASMQRDEVRESLRMWTGTQRPDFEGRGNVT
                     PAAERVTLSDAGRAAQAAEAGAANASLDDSENDPRLLLLRAIIRMLTGEEARVFDAAS
                     LDGPSPATEASTERGTATQSDNAAPTPAERPAGYGVEYDYHASHSETELTSFAASGVV
                     KTADGKEIHFDLQLSMARSYHQENDVSLRLGDATRQRKDPLVLNFAGTAAQLSDQRFK
                     FDLDSDGTADSVNLLTPGSGFLTLDRNKDGRINNGSELFGTRSGDGFADLAALDGDHN
                     GWIDENDAAYAQLGVWIPDANAGGKLRSLQEANVGALSLARVATPFDIRDGGNQTLGQ
                     IRSSGVFLQESGGVGSMQQI"
BASE COUNT          888 a         1448 c         1247 g          863 t
ORIGIN      
        1 ccttcgacca gtaccttcac ggtcccgtcc ggcagtttca acatctgcag gatattggcc
       61 acacagccca cactgtagag gtcttctgct tccggctcgt ccttgacggc ggacttttga
      121 gccaccagca ggatgctctt gccgacttcc atcgccactt cgagggcctt gatcgacttg
      181 gggcgcccga caaacagagg aatcaccatg tgcggaaaaa ccaccacgtc gcgcaggggc
      241 aacagcggca gttcgactgg ctcgctaaac agcttggggg ttgcagacat cgattgtgcc
      301 cttcaggtta aagtccatgc cgtgatatgg tggcaagaac tcgtaattca agccgcacag
      361 agtgcggcca gcacatcagt tgccgccgga aaccttgggt tgatcagcgt agatcaggat
      421 tggacgtgca tcaccgacga tcacgctctc gtcaatgacg acctctgaga cattctccat
      481 accgggaagt tcgtacatgg tgtcgagcag caccgactcg ataatcgaac gtaggccgcg
      541 cgcaccggtc ttccgttcga gcgcctttct ggcaatggcc atcatcgctg ccggacggat
      601 ttccagcgcc acaccctcca tcgcgaacaa tttttggtac tgcttgacca gggcgttctt
      661 cggttcgacc aagatttgta ccagcgccgc cgtcgataat tcctcaagtg tggcgagcac
      721 cggcaggcgc ccgatgagtt cgggaatcag gccgaacttg atcaggtctt cgggttcgac
      781 gctgcgcagc acatcgccaa tcgccttctt gttctccttg cttttcacct cggcaccaaa
      841 gccgattccc ccacgctcgg agcggtggcg gatgaccttc tccagaccgt caaaagcgcc
      901 accgcaaatg aacagaatat tcgtggtatc gacctgcacg aagtcctgat tgggatgctt
      961 gcgccccccc tgcggcggga ccgatgccac cgtaccttcg atcagcttga gcagcgcttg
     1021 ttgcacaccc tcaccagaaa cgtcgcgagt aatcgaagga ttgtcggact tgcgcgaaat
     1081 cttgtcgatc tcgtcgatat agacgatccc gcgctgggcc ttctcggtat cgtaatccgc
     1141 cttctgcagc agcttctgga tgatgttttc gacatcctct ccgacgtagc cggcttcggt
     1201 cagggtcgtc gcatcggcca tcacgaaagg cacgtcgagc atgcgcgcca gcgtctgcgc
     1261 cagcaaggtc ttgcccgacc cggtcgggcc gatcagcagg atgttgctct tggcgagttc
     1321 aatctcgtcg ctgcttttgc cgaagtgacg caaccttttg taatggttat acaccgccac
     1381 cgacagaatg cgcttcgcct gatcctggcc gataacatac tggtcgagga gtgccgagat
     1441 ctcccgtggc gtcggcaggt cccccttggc acccttggca cccagatcac cggcaatctc
     1501 gtcccgaacg atatcgttgc agagttcgat gcattcatcg cagatgaata ccgacggccc
     1561 ggcaatcagc ttcttcactt catgctggct cttgccgcag aacgagcagt agagcaattt
     1621 ttcagtgcca ctcttctttt ccgacatgtc gacctacctc gctgtcaagc cttatggaga
     1681 ctgcccggat gcggcgtcaa atcgcatccc gtttggtcag caccttgtcg atcaaaccat
     1741 attctgccgc ttcctgcgcg gaaaggaaat tgtcgcgatc ggtatcgcgc tcaatgcgnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cgacttgacg acttcggcaa
     1921 agatcaggcc gagcgttacc cgccgccggg cctggtcgac aaaccactcg ggctgcatcg
     1981 gcaaatcctt gaccttcatg ccgcgctgtt ccatgtcctg ccgcgccaac tgcatcagac
     2041 gctcgatctc gcggtggatc agtgctcccg gcacctcgat ggcattgacc cgcagcagcg
     2101 catccatcac ctgttccatg atcttggcct gcagacgctt cttcacctcg cgccggaggt
     2161 tgtcctcgat ttcggcccgc atcttgtcga gatcaccatc cgcgacgccc agggccttgg
     2221 cgaagtcggc gtcaacttcc ggaagaatcg cctcacccac ctctttgacc gtcagttcga
     2281 aggtcaccgt ctggccggcc agttccttcg agaaatactc ggcgggaaag gtcagttcga
     2341 aggatttgct ttcacccggg cgcgtaccga tcaccgcgtt ctcgaaatcg gaaagcatct
     2401 tgccttcgcc gagcacgaaa cgataatcct tgccctcgcc tccggcgaac ggctcaccgc
     2461 ccttcttgcc gagaaaatcg acgctcacgc ggtcagcgtt cgccgccgga cgatcgacga
     2521 gttcgtaacg cacacgctgc ttgcgcagaa tgtcgatcgt cccgtcgatc tcggcgtcac
     2581 ccacttcaag taccggtcga gagatctcca ccccggtcag gtcgcccagg gtgatctccg
     2641 gatagacttc gaagaccgcc gaaaactcga tatgcgtcgt actgccgctg tccctgggct
     2701 cgatcttcgg actgccggcg acgcgcaact gccgcgctgt gacctcgtcg ctcaacttct
     2761 gccccagagc ctcggtcaac gcctcatggc gagcctgctc gccatactgc tgcctgacga
     2821 tgtgaatcgg caccttgccc ggacggaagc ccggcatctt catgttccga cccagacgct
     2881 gcaggcgctg gtcgacttcc ctgtcgagtt cggcaatcgc gaccgaaagg tcgacccggc
     2941 gctcaagagc gcttacggct gcgacaatgg ttgatgggtc agtgttggcg acgttcgttt
     3001 ccattaagaa tccttgtgat acgggctgaa aggtgactga aacctggctc ggacgaaggc
     3061 gcccgcgccg ccagacgatg ctgcgccggc agccggaccg aaaatgcgga gaacatcgtg
     3121 ctgctccagc attcgttcgc ttccagggaa cttcacgagg caagcggtct tccagcgcac
     3181 cagtaaaaga cccgcatgcc cacccgggag gcatagacaa cggcgtctgc tttggttcca
     3241 tgtcggggtc cgcgctgcaa tgccgcctct ccgcgcacgg aaagtttgcg attctacagc
     3301 aataattgat ggctcgaaac ccgtggcgtt ctgtcgaggc ggccgcctcg acgcggcgga
     3361 actggtcccg ctccggtaaa ccgcgtcagc tattaccgag cgcgcgcact ggcagcgaaa
     3421 tgtattcaag aaagcgcccg cttcggccgt taagcattta ccaaccccac gaggaagccg
     3481 ccatgaagat caccagttcc gagatgcaga tggtttcgtc gcatgcctcg atgcaacgtg
     3541 acgaagtccg cgaatcgctg cgcatgtgga cgggaacaca gcggccggac ttcgaaggcc
     3601 gtgggaacgt aactcctgct gcggagcggg taacgctctc ggatgccggc agagcggcac
     3661 aggcggccga ggctggtgcc gcgaacgcca gcctggacga cagcgagaac gatccacgac
     3721 tgctgctgtt gcgcgccatc atccgcatgt tgaccggaga agaggcgcgc gttttcgatg
     3781 cggcctcact cgacggcccg tccccggcga cggaagcgtc gaccgagagg gggaccgcca
     3841 cccagagtga caacgcggct ccgaccccgg ccgagcggcc ggccggttat ggtgtcgaat
     3901 acgactacca cgcctcccac agcgaaaccg aactgacctc gttcgcggcc agcggagtgg
     3961 tgaaaacggc cgatggcaag gaaatccact tcgacctgca actctcgatg gcacgcagct
     4021 accaccagga aaacgacgtc agcctgcgcc tgggcgacgc gacgcgccag cgcaaggacc
     4081 ccttggtgct caacttcgcg ggcacggccg cccaactcag cgaccaacgc ttcaagttcg
     4141 acctggactc cgacggcacc gccgacagcg tcaatctgct cacccctggc agcggattcc
     4201 tcaccctcga tcgcaacaag gacggccgga tcaacaacgg cagcgaactt ttcggcacgc
     4261 ggagcggcga cggctttgcc gatctcgccg cgctcgacgg cgaccacaac ggctggatcg
     4321 atgaaaacga cgcggcctat gcccagttgg gagtctggat acccgatgcc aacgcaggcg
     4381 gcaagctacg cagcctgcag gaagccaatg tcggcgccct cagcctggcc cgcgtcgcca
     4441 cccccttcga catccgtgac ggcggcaacc agaccctcgg ccagatccgc agcagcggtg
     4501 tcttcctgca ggaaagcggc ggggtcggga gcatgcagca gatcga
//
LOCUS       DAOSIT010000141         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16847_length_4542_cov_7.979719, whole genome shotgun sequence.
ACCESSION   DAOSIT010000141 DAOSIT010000000
VERSION     DAOSIT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16847_length_4542_cov_7.979719"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="der"
                     /locus_tag="PLL14_04715"
     CDS             complement(<1..1318)
                     /gene="der"
                     /locus_tag="PLL14_04715"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG03256.1"
                     /translation="MKPSIVLVGRPNVGKSTLFNRLTRTRDALVADLPGLTRDRHYGH
                     GRLGSKPYLVIDTGGFEPLAKDGIMYQMARQTEQAIDEADVVVFVVDGRSGVTALDKE
                     IANRLRKSGRPVLVAVNKSEGMSQGIVVSDFHELGLGQPLAISAAHGEGVRTLIDEAL
                     DPYSDQMEGETTSDVVKVAIVGRPNVGKSTMINALLGEDRVIAFDQPGTTRDAIHVDF
                     ERGGRRYTLIDTAGLRRRGKVFETLEKFSVIKTLQAIEEAQVVILVLDARQDISDQDA
                     HIGGFIRESGRALVIAVNKWDGLDAYTREQLKGALENQLSFIDFARFHYVSALKGHGL
                     EALFRSVDAAYKAATADLPTPKLTRTLIDAVARQAPPRSGLFRPKLRYAHQGGRNPPL
                     IVIHGNALDKVPESYRRFLEHTFREVFQLQGTPLQIQFNVSSNPFAD"
     gene            complement(1315..2457)
                     /gene="bamB"
                     /locus_tag="PLL14_04720"
     CDS             complement(1315..2457)
                     /gene="bamB"
                     /locus_tag="PLL14_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HOG03257.1"
                     /translation="MKRCMLAAACLSALLAGCSTLDALNPFASSGPKMAELQPIQATA
                     EVRVLWNNTVGKAEELNFVPAVVDASVYAAARDGTVARFDDGQQVWRIKLEQPLSGGV
                     GADERRFVVGTAKGEILAFATADGSPLWKARASSEIVAPPLVRDGLVIVRSGDHRLAA
                     YDAVDGKRKWIYQRPSTPLSLRATASPVLVEKYVFAGFPGGKLIAVSADNGAPLWEGA
                     VALPKGATELDRVADVSSMPVIDARMICAAAFQGRVSCFDLGSGSLVWAREISSAAGL
                     AIDSRFVYVSDDKGAVHALDKASGASLWKQDKLFLRRLTAPMPLRNLVVVGDVRGVVH
                     FLNRDDGSFVARLPTDGSPLRAPLQRLGSNLLLQTSNGSVLAIEVQ"
     assembly_gap    2511..2610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2611..3011)
                     /locus_tag="PLL14_04725"
     CDS             complement(<2611..3011)
                     /locus_tag="PLL14_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG03258.1"
                     /translation="MATYDLEEQEQIAEIKAWWQQYGNLLAGIVTAAAIVVVAWQGWN
                     WYQRNQAAQASMVYGSLQKAVRDNDLQRIKASSGELLEKFGGTAYAPMAAMTTARMLF
                     AAGDAKTAKLQLLWVVEHGQDEFRDLARLRLA"
     gene            complement(3011..4306)
                     /gene="hisS"
                     /locus_tag="PLL14_04730"
     CDS             complement(3011..4306)
                     /gene="hisS"
                     /locus_tag="PLL14_04730"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOG03259.1"
                     /translation="MTPKIQSVRGMNDILPDEAEFWDLFEETIRSWLKSYGYRPIRLP
                     IVEPTPLFKRAIGEVTDIVEKEMYSFVDALNGEALTLRPEGTAGCVRAAIQHNLVAQQ
                     PQRLYYMGQMFRHERPQKGRYRQFHQVGVEALGFAGPDIDAEQILMGARLWGDLGLDG
                     VALQINTLGQAAERTRHRSELIGYLERHGELLDEDARRRLHSNPLRILDSKNPALQEI
                     IRDAPQLIDHLGTESMAHFESVQQVLRDAGQPFEINPRLVRGLDYYNLTVFEWVTDRL
                     GAQGTVCAGGRYDGLVQQLGGKSVPACGFAMGVERLLALLREAGGETTAAAVDVYLVH
                     QGSAASRLAVRVAEGLRDQGIDVLFHCGGGGFKAQMKKADASGANFAVIIGDDEAEAG
                     EVTLKALRANGEQPNEQKRVSVDGLADAIMGSILDWEES"
     gene            complement(4309..>4542)
                     /locus_tag="PLL14_04735"
     CDS             complement(4309..>4542)
                     /locus_tag="PLL14_04735"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG03260.1"
                     /translation="GVENMTLAVMGCVVNGPGESKHANIGISLPGTGEVPAAPVFEDG
                     AKTVTLRGEHIAEEFTAIVDAYVARTYQRKFAG"
BASE COUNT          853 a         1455 c         1299 g          835 t
ORIGIN      
        1 tgtcggcaaa cgggttgctg gaaacattga actggatttg cagtggcgtc ccctgcaact
       61 ggaaaacctc ccggaaggta tgttcgagaa agcgtctgta cgactccggg accttgtcca
      121 gagcattgcc atggatcacg atcagcggcg ggttcctgcc accctgatga gcgtaacgta
      181 gcttcggtcg gaacagaccg ctgcgcgggg gtgcctgtct ggcgaccgcg tcgatcaggg
      241 tacgcgtcag cttcggcgtt ggcaggtcgg cagttgccgc cttgtaggcc gcatcgaccg
      301 aacggaaaag cgcttcgagc ccatgcccct tgagcgcgga cacatagtgg aaacgcgcga
      361 aatcgatgaa cgacaactga ttttcgagag cgcctttgag ttgctcgcgg gtgtaggcat
      421 cgaggccgtc ccacttgttc acggcgatga ccagcgcacg gccggattca cgaatgaaac
      481 cgccgatatg cgcatcctgg tcggagatgt cctggcgtgc gtcgagcacc agaatcacca
      541 cctgcgcctc ctcgatcgcc tgcagcgtct tgattaccga gaatttctcc agggtttcga
      601 acaccttgcc acgacggcga aggccggcgg tatcgatgag cgtataccgc cggccgccgc
      661 gctcgaaatc gacatggatg gcgtcgcgag tcgttcccgg ttgatcgaag gcgatgacgc
      721 gatcttcgcc gagcagcgcg ttgatcatcg tgctcttgcc gacattcggg cggccaacga
      781 tggccacctt caccacgtca ctggtggtct cgccctccat ctggtcagaa tacggatcga
      841 gtgcctcgtc gatcagcgtc cgcacaccct cgccatgcgc cgccgagatc gccagcggct
      901 ggccgagacc gagttcatga aaatccgaga cgacgatccc ctggctcatg ccttcggact
      961 tgttgaccgc caccaggacc ggccgcccgg atttgcgcaa gcggttggca atttccttgt
     1021 cgagcgcggt caccccgctg cggccgtcga cgacaaacac cacgacatcg gcctcgtcga
     1081 tcgcctgctc ggtctggcgc gccatctggt acatgatgcc gtctttggcc agcggctcga
     1141 agccaccagt atcgatcacc agatagggct tgctgccaag cctgccatga ccgtagtgac
     1201 ggtcacgcgt gaggcccgga aggtcggcca ccagcgcgtc gcgagtccgg gtcaggcggt
     1261 tgaaaagtgt cgacttgccg acattgggac gtccgaccag aacgatgctc ggcttcattg
     1321 cacctcgatt gccagcacgc ttccgttact ggtctgtagc aacagattgg atcccaggcg
     1381 ctgcagtggc gcgcgcagcg ggctgccgtc agttggcaag cgggccacga aggagccgtc
     1441 atcgcgattc aggaagtgca cgaccccgcg cacatcgcct acgacaacca gattgcgcag
     1501 cggcatcggc gccgtaagcc ggcgcaggaa gagcttgtcc tgcttccaga gactggcacc
     1561 gctcgccttg tcgagcgcat gcacggcacc cttgtcgtcg gacacgtaaa cgaaccgact
     1621 gtcgatcgcc aaaccggcag cagacgagat ctcgcgtgcc cagaccaggc tgccgctgcc
     1681 caggtcgaag caggaaacgc gcccctggaa ggcagccgcg cagatcattc gcgcatcgat
     1741 gaccggcatg ctgctgacat cggcaacgcg atcgagttcg gtggcgccct tcggaagagc
     1801 caccgctccc tcccacagcg gagcgccgtt atctgcgctc accgcaatca atttaccgcc
     1861 ggggaagccg gcaaagacgt acttttcgac cagcaccgga ctggcggtcg cgcgcagtga
     1921 cagcggcgtg ctcggacgtt ggtaaatcca cttgcgctta ccgtcgacgg catcatatgc
     1981 cgccaggcga tggtcgccac tgcgcacgat caccaacccg tcgcggacca ggggcggggc
     2041 aacgatctcg ctgctggccc tggctttcca caaggggctg ccgtcagccg tggcaaaagc
     2101 caggatctca cccttggcgg taccgaccac gaaccgtcgc tcatcggcgc cgacgccacc
     2161 cgaaagcggc tgttcgagct tgatccgcca gacttgctgg ccatcgtcga agcgggcgac
     2221 cgtgccgtcc cgagctgccg cgtaaaccga ggcatccacc accgccggga cgaagttgag
     2281 ttcctccgcc ttgccaaccg tgttgttcca aagcacacgc acttcagcgg tcgcctggat
     2341 cggctgcagt tcggccatct tcggtccact gctcgcaaac ggattaaggg cgtcgagcgt
     2401 cgagcaaccg gccagcaaag cagacaggca ggctgctgcg agcatgcacc gcttcatcag
     2461 ttgggctccc caagcgcatc ccgcttcaga tgcaggattt cacgataggc nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gccaggcgca aacgtgccag atcacggaat
     2641 tcatcctggc catgctcgac tacccacagc aactgcagct tggcggtctt ggcatcgccg
     2701 gcagcaaaca gcatcctggc agtggtcatg gcggccattg gggcataggc cgtgccacca
     2761 aacttttcaa gcagttcgcc actgcttgcc ttgatgcgct gcagatcgtt gtcgcgaacc
     2821 gccttctgta gtgagccgta taccatcgac gcctgcgccg cctgattgcg ttgataccag
     2881 ttccagcctt gccaggcgac aacgacgatc gcggccgcag tcacgatgcc ggccaggagg
     2941 ttgccgtact gctgccacca ggccttgatt tcggcaattt gttcctgttc ttcgaggtcg
     3001 taagtagcca ttacgattct tcccaatcca aaatagaacc catgattgcg tcggcaaggc
     3061 catcgacact gacccgcttc tgctcgttcg gttgttcccc attcgcgcgc agtgccttga
     3121 gcgttacctc gccggcttct gcctcgtcgt cgccgatgat cacggcaaaa ttcgcccccg
     3181 aggcatcggc cttcttcatc tgcgccttga agccaccacc accgcaatgg aacagcacgt
     3241 cgatgccctg atcacgtagc ccctcggcga cccgtaccgc gagacgcgaa gcagcgctgc
     3301 cctgatgcac aagatagacg tccactgccg ctgcggtggt ttctccacca gcctcgcgaa
     3361 gcagcgccag caaccgctcg actcccatgg cgaaaccgca ggccggaacc gacttgccgc
     3421 ccagttgctg caccaggcca tcgtaacgtc cgccggcaca gactgttccc tgcgctccca
     3481 gccgatccgt gacccactcg aaaacggtca ggttatagta gtcgagacca cgcaccaggc
     3541 ggggattgat ctcgaagggc tgacccgcat cgcgcagcac ctgttgcacg ctctcgaaat
     3601 gcgccatcga ctccgtaccc agatggtcga tcagctgggg ggcgtcgcga atgatctcct
     3661 gcaacgccgg gttcttgctg tcgagaatac gtagcgggtt gctgtgcaag cgtcgtctgg
     3721 cgtcttcgtc gagaagttcg ccgtgccgct ccaaataacc gatcaactcg gagcgatggc
     3781 gcgtccgctc ggccgcctga cccagtgtgt tgatttgcag tgctacgccg tcgaggccga
     3841 ggtcgcccca caaacgcgca cccatcagga tctgttcggc atcaatgtcg ggaccggcaa
     3901 agccaagcgc ttcgacgccg acctgatgaa actggcgata acgccctttc tgcggccgct
     3961 catgccggaa catctggccc atatagtaga gccgctgcgg ctgttgcgcc acgagattgt
     4021 gctggatggc ggcgcgcacg cagccggcag taccctcggg acgcaatgtc agtgcttcgc
     4081 cgttgagcgc atcgacaaag gaatacatct ccttctcgac aatgtcggtc acttcgccga
     4141 tggcacgctt gaacagcggt gtcggctcga cgatcggcag gcggatcggg cggtaaccat
     4201 agctcttgag ccacgagcgg atggtttcct cgaaaaggtc ccagaattcg gcctcgtcgg
     4261 gcaggatatc gttcatcccg cgcaccgact ggatcttcgg agtcatcatc agccggcaaa
     4321 ctttctctgg taagtacgcg ccacgtaagc atcgacgatg gcggtgaatt cttcggcgat
     4381 gtgttcgccg cgcagggtaa ccgtcttggc gccatcttcg aacaccggtg cggccggaac
     4441 ttcgccggta cccggcaggc tgatgccgat attggcatgc ttgctctccc ccggcccatt
     4501 gacgacgcag cccatcaccg ccagcgtcat gttctcgacg cc
//
LOCUS       DAOSIT010000142         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_16858_length_4539_cov_9.403434, whole genome shotgun sequence.
ACCESSION   DAOSIT010000142 DAOSIT010000000
VERSION     DAOSIT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_4539_cov_9.403434"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /gene="mraW"
                     /locus_tag="PLL14_04740"
     CDS             <1..350
                     /gene="mraW"
                     /locus_tag="PLL14_04740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOG03261.1"
                     /translation="AATRTFQALRIHINQELEQLALVLPQAMTVLKQGGRLVVISFHS
                     LEDRIVKHFMRDEAVPDHAPRKLPLRAAELPQPRLRLLGKPLRASAAEVEANPRARSA
                     VMRVAEKYAGRAA"
     gene            352..630
                     /gene="ftsL"
                     /locus_tag="PLL14_04745"
     CDS             352..630
                     /gene="ftsL"
                     /locus_tag="PLL14_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOG03262.1"
                     /translation="MVRLNVFLLLAVVICSLGVVTSQHKARKIFQALEVEQERARQLE
                     VEFGQLQLELSTWGTAPRIEKIAREKLKMRTPETERVITATPAGGLTR"
     gene            627..>2311
                     /locus_tag="PLL14_04750"
     CDS             627..>2311
                     /locus_tag="PLL14_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG03263.1"
                     /translation="MMRFKGKVAHKFAESPLLQMRLPLWRSRLMALLILGSFAVLIGR
                     AFYLQTLNNDFLQEKGESRYRRDIEISASRGRIADRHGDVLAISTPMKSIWAIPPATT
                     LTPVQTRQLAALLETDPKQLAHKLDTDKTFVFLRRQIPPPVAEQVAALKLPGIGQDKE
                     YRRFYPTGEMTAHMVGFTGVDDKGLEGVELAFHGQLLGQPGSRSVIKDRRGQIVEDVG
                     SIKQPQDGKEIRLALDSKIQYLAYSHLKQAIAENNAKAGGVVVLDVRTGEIVALANWP
                     TYNPNNREGLSGGQLRNRAVTDTFEPGSTLKPFTIGLALENGKVRSDTVINCAPGRLT
                     IGSATISDAHPHGALTVAEVIQKSSNVGAAKIAAMLPAQKMWQMFDDVGFGQVPRLGF
                     PGEVSGRVRPWKNWRPIEQATMSYGHGISVSLIQLARAYSVFARDGDLMPLSLTRLDS
                     STPTGAAVFSPKTAREVRSMLEMAVLPGGTAPKAQIPGYRVAGKTGTAHKLEGGRYAN
                     KFVSSFVGFAPASDPRLIVAVMIDEPSGGKHYGGDVAAPVFAAVMGNSLRTLG"
     assembly_gap    2312..2411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2412..3593
                     /locus_tag="PLL14_04755"
     CDS             <2412..3593
                     /locus_tag="PLL14_04755"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG03264.1"
                     /translation="RPSERLSLVAVTGTNGKTSVTQWIGSTHPRRCPIIGTLGAGFPG
                     QLLETGFTTPEATTLMRFLADFADQEAQACALEASSIGIAEGRLDGARVDVAVFTNLT
                     RDHLDYHGSMEQYAQAKARLFTWPRLRLAISNLDDPCGRALAALSTATKVIGYTQQDL
                     PADRQATIRAEEVEETIDGLRFRLCAPNGRALVETGLLGRYNVSNLLAVAAVLLDAGL
                     TPAAVAERFAGLTPPAGRLEKIGGHNEPLVVVDYAHTPDALESALRALRPVASARGAG
                     LTVVFGCGGDRDRGKRPLMGEIAVRHADRVVLTSDNPRSEDPQAILDDIHAAAPAAES
                     IVDRGEAIRRTIVAAHSADVVLLAGKGHEPYQEIAGVRRPFSDVTQARAALDARRESE
                     R"
     gene            3590..>4539
                     /gene="murF"
                     /locus_tag="PLL14_04760"
     CDS             3590..>4539
                     /gene="murF"
                     /locus_tag="PLL14_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOG03265.1"
                     /translation="MSVMMDLLSAARATAGKLLGDNRTFCGVSTDSRSVADGELFVAL
                     RGDNFDGHDYFAAAAARGAVAAVVAADAAEGLQALGLPLVQVAETRLSLGALAADWRC
                     RFTLPLIAVTGSNGKTTTKEMIASILNTAFPAAVLSTHGNYNNDIGLPLTLLRLNATH
                     RAAILEMGMNHPGEIAYLAGIARPTVALVTNAQRAHLAGMGSLEAIAAEKGSIYSGLD
                     ESGVAVFNADDAWSALWRDQSRGLATMTFGFEQPAEVTGRFYLHGLESRLSVCAPGGQ
                     FDVLLALPGQHNARNALAAATACLAAGVSLADVQAGLASFR"
BASE COUNT          783 a         1384 c         1461 g          811 t
ORIGIN      
        1 acgcggcgac gcgtaccttt caagctctac ggattcatat caatcaagag ctcgagcaac
       61 tcgcgttagt gctgccgcag gccatgaccg tcttgaaaca gggagggcgc ctggtggtga
      121 tcagtttcca ttcgctcgaa gaccggatcg tcaagcactt catgcgcgac gaggcggttc
      181 ctgaccacgc gccgagaaag ctgcccctgc gtgccgcgga gttgccgcag cccaggctgc
      241 gtctgctggg caagccgctc agggcaagcg cagccgaggt cgaggccaat ccgagggccc
      301 gcagcgcggt gatgcgtgtc gccgaaaaat acgccgggag ggctgcctga gatggttcga
      361 ctgaacgttt ttctgttgct tgccgtcgtc atctgcagtc tcggagtcgt gacttcacaa
      421 cacaaggcac gcaagatttt tcaggcgctt gaggttgaac aggagcgcgc gcggcaactc
      481 gaggtcgaat ttgggcagtt gcagctggaa ctttcgacct ggggcaccgc gccacgtatc
      541 gagaagattg cccgcgagaa gctcaagatg cggacgccgg aaacggaacg ggtgattacg
      601 gcaacccctg ccggaggact aacgcgatga tgcgtttcaa aggaaaagtg gcacacaagt
      661 ttgccgagag ccccttgctg caaatgcgtc tgccactctg gcgttcgcgc ctgatggccc
      721 tgctcattct gggctctttt gccgtgctga tcggacgtgc cttctaccta cagacgctga
      781 acaatgactt cctgcaggaa aagggcgagt cacgctaccg gcgcgatatc gaaatctcgg
      841 cctcgcgtgg gcgaattgct gatcgtcacg gcgatgttct ggccatttcg acgccgatga
      901 agtcgatctg ggccatcccg ccggcgacga cgttgacgcc ggtgcagacc aggcagttgg
      961 ccgctctgct ggaaaccgac cccaagcaac tggcccacaa gctcgacacc gacaagactt
     1021 tcgtttttct tcgccggcag attccgccgc cggtggccga acaggtggcg gcgctgaagt
     1081 tgcccgggat tggtcaggac aaggagtacc gtcgcttcta tccgaccggt gagatgactg
     1141 ctcacatggt gggttttacc ggcgtcgacg acaaggggct cgaaggcgtc gaactggcct
     1201 tccacgggca actgctgggc cagccgggta gccgcagcgt gatcaaggat cggcgcggcc
     1261 agatcgtcga ggatgtcggt tcgatcaagc agccgcagga tggcaaggaa atccgtctgg
     1321 cgctcgattc gaagattcag tacctggcct acagccacct caagcaggcg atcgccgaga
     1381 acaatgccaa ggctggcggg gtcgtcgtgc tcgatgtgcg caccggcgaa atcgtcgcgc
     1441 tggccaactg gccgacctac aatccgaaca atcgcgaggg cctgtccggc gggcagttgc
     1501 gcaaccgcgc cgtcaccgat acttttgagc ccggctcgac gctcaaacct ttcaccattg
     1561 gtctcgccct cgagaatggc aaggtgcgct cagacaccgt catcaactgc gcgcccggtc
     1621 ggctgacgat cggcagtgcg acgatttccg acgcccatcc ccacggtgcg ctgacggtgg
     1681 ccgaagtcat ccagaagtcg tcgaacgtcg gagcggccaa gatcgccgcc atgttgcccg
     1741 cgcagaagat gtggcagatg ttcgacgatg tcggtttcgg tcaggtgcca cgcctgggct
     1801 tccccggtga agtgagcggc cgggttcgtc catggaagaa ctggcggccg atcgaacagg
     1861 caacgatgtc ctacggccat ggcatttccg tgtcgctgat ccagttggcg cgcgcctatt
     1921 cggtgtttgc tcgcgacggc gatctgatgc cgctctcgct gacacgactc gattcctcca
     1981 cgccaaccgg cgccgcggtg ttcagcccga agaccgcacg cgaagttcgc agcatgcttg
     2041 aaatggcggt cctgccaggc gggacggcgc ccaaggcgca gattcccggg taccgggtgg
     2101 ccggcaagac gggtaccgca cacaagctcg aaggcggtcg ttacgccaac aagttcgtct
     2161 cctcgtttgt cggtttcgcg ccggcatcgg acccgcggct gatcgttgcc gtcatgatcg
     2221 acgaacccag tggtggcaag cactatggtg gtgatgtcgc ggcgccggtg tttgccgcgg
     2281 tgatgggcaa ctcgctacgc accctcggca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn ncgtccaagc gagcgcttgt cgctggtcgc cgtcaccggc accaacggca
     2461 agaccagcgt cacgcagtgg atcggcagca cccatccgcg tcgttgtccg atcatcggta
     2521 cgctgggagc cggctttccg ggtcaattgc tcgaaaccgg gttcaccacc cccgaggcga
     2581 cgacgctgat gcgcttcctg gccgactttg ccgaccagga agcgcaggcc tgcgcgctcg
     2641 aagccagctc gatcggtatc gccgaagggc gccttgacgg cgcacgcgtc gacgtggcgg
     2701 tgtttaccaa tctgacgcgc gatcatctcg attaccacgg cagcatggag cagtacgcgc
     2761 aggccaaggc gagactgttc acctggccgc gcctgcgcct ggcgatcagc aatctcgacg
     2821 acccctgcgg gcgtgcgctg gccgcattga gcacggcgac caaggtcatc ggctacaccc
     2881 agcaggatct gcccgccgat cggcaggcga cgatccgtgc cgaggaggtc gaggaaacca
     2941 tcgacggcct gcgtttccgc ctctgcgcgc cgaatgggcg tgcgctcgtc gagaccggcc
     3001 tgctcggtcg ctataacgtg tcgaatctgt tggccgtggc cgcggttctg ctcgacgccg
     3061 gcctgacgcc ggcggcggtt gccgagcgct ttgccggcct gacgccgccg gccggtcgcc
     3121 tggaaaagat cggcggtcac aacgagccgc tggtggtggt cgactatgcg cacactcccg
     3181 atgccctgga gagcgccttg cgggccttgc ggccggtggc gagtgcgcgc ggcgcgggtc
     3241 tgaccgtggt gttcggttgt ggtggcgatc gcgatcgcgg caaaaggccg ttgatgggcg
     3301 aaatcgcggt acgccatgcc gaccgtgtgg tgctgaccag cgacaacccg cgtagcgagg
     3361 atccgcaagc cattcttgac gacatccacg ccgctgctcc ggccgccgag agcattgtcg
     3421 atcgtggcga agcgattcga cgcaccattg ttgccgcaca ttccgccgac gtggtgctgc
     3481 tggccggcaa ggggcacgag ccctatcagg agatcgccgg tgtgcggcgg ccgttttcgg
     3541 acgtgacgca ggcgcgtgcg gcgctcgatg cccgccggga gagcgagcga tgagcgtgat
     3601 gatggatctg ctgagcgctg cacgcgccac tgccggcaag ctgctcggcg acaatcggac
     3661 cttttgcggt gtatcgaccg atagtcgcag tgttgccgac ggcgagctgt tcgttgccct
     3721 gcgaggtgat aatttcgatg gccacgatta cttcgccgcg gccgccgcgc gtggcgcggt
     3781 tgccgccgtg gttgccgcag acgcggccga aggtttgcag gcgttgggct tgccactggt
     3841 gcaggtcgcc gagacgcggt tgtcgctggg ggcgctggcc gccgactggc gttgccgttt
     3901 cacgctgccg ctgatcgccg tcaccggcag caacggcaag acgacgacca aggaaatgat
     3961 tgccagcatc ctgaacacgg ccttccccgc agcggtcctg tcaacccacg ggaattacaa
     4021 caacgacatc ggattgccac tcacgctgct ccggttgaac gcgacacatc gcgcggcgat
     4081 tctcgagatg ggcatgaacc accccggtga aatcgcgtat ctggcgggaa tcgcacgtcc
     4141 caccgtggcc ctggtgacca atgcccaacg cgcgcatctg gccgggatgg gctcgctgga
     4201 ggcgattgcc gcagaaaagg ggagcatcta cagtgggctc gatgaaagcg gtgtcgctgt
     4261 gttcaacgcc gatgacgcct ggtctgcgct ttggcgcgac cagagccgcg gcctggcgac
     4321 gatgaccttc ggctttgagc agccggcgga ggtgaccggc aggttctacc tgcatggtct
     4381 cgaaagtcgt ctcagcgttt gcgctccggg tggccagttc gatgttctgc tggccctgcc
     4441 ggggcagcat aacgcgcgca acgctctggc cgcagccacg gcctgcctgg cggcaggtgt
     4501 atcgctggcc gacgtgcagg ctggtctggc cagctttcg
//
LOCUS       DAOSIT010000143         4503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17037_length_4503_cov_10.750674, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000143 DAOSIT010000000
VERSION     DAOSIT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17037_length_4503_cov_10.750674"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLL14_04765"
     CDS             <1..478
                     /locus_tag="PLL14_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG03266.1"
                     /translation="NCGVPGIGLKWPNDILLDGGKLGGILVELDAQPDGMVAVMGIGL
                     NLLLPAAGAEEFLHRPASLSQASSPPPDRHQLLARILLELAVVLDRFAVAGFSVLREE
                     WQAHHVWQERAVRLLNGGLLDREGICLGADSDGALLLRTAAGIERCLSGDLSLRAA"
     gene            475..1194
                     /locus_tag="PLL14_04770"
     CDS             475..1194
                     /locus_tag="PLL14_04770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG03267.1"
                     /translation="MNIAIDAGNSRIKWGVHDGTGWLDSGVLATADVAWLSEAADEWP
                     VDARVMVCNVAGSEVAATLSALLAGRHPRVSFLHASAEACGVRNTYAFPGQLGADRWA
                     ALIGARALLEHACLVVCAGTATTVDLLDADGVFRGGLILPGFDLMRAALADNTAQLPL
                     AEGKFRSEPRNTMDAIVSGCLQAQLGAVERMFATIATAPGARCLLTGGAAAPLAAHLK
                     IPFQLADNLILNGLVRYAESL"
     gene            1228..1632
                     /locus_tag="PLL14_04775"
     CDS             1228..1632
                     /locus_tag="PLL14_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOG03268.1"
                     /translation="MPAHLMTTLPAFLAYFAVAMVLLALFLLVYLNVTPYHELALIRA
                     GNSAAAVSLSGALLGFAMPVANVIAHSDTLIDLASWGAIAGIIQILAYLVARFTLPQL
                     NQDIPADKLAPAIFLAAISLGVGVINAACMGY"
     gene            1696..2730
                     /locus_tag="PLL14_04780"
     CDS             1696..2730
                     /locus_tag="PLL14_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG03269.1"
                     /translation="MALMDFIRKQFIDVIHWTEEGDGVLAMRYPMRDFEIQYGAQLTV
                     RESQIAVFVNEGQIADVFQPGQYHLTTRTLPVLTYLKNWDKLFESPFKSDVYFFSTRL
                     QLDCKWGTPNPITIRDKDFGMVRMRAFGIYSYKLADARKFHREISGTREQYTVTDLDG
                     QLRNLVISSMTDLFGESGVPFIDMAANQNELSRTLKDKLEAVFDRYGLALDSFVVQNV
                     SLPEELQKILDTRIGMNMIGDLGRYTQYQVANSIPLAAQNEGGIAGLGAGLGAGLGIG
                     QTMTTTVAQALQTGANPPVTAAALAPDSAGSADAVVATLEKLHSLVSKGILSQAEFDA
                     KKAELLARLQ"
     assembly_gap    2763..2862
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2863..>4503
                     /locus_tag="PLL14_04785"
     CDS             <2863..>4503
                     /locus_tag="PLL14_04785"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOG03270.1"
                     /translation="IWNEWHILFDDGRGAWLAEAAGEWIVSAQVAVADPLPAFETLAP
                     DLPVTLDGRSFRVADLETARCIAGQGELPFKVAAGYDVNTADLRGNGRYVTLDYSETP
                     PLVFVGQAVSFADLQLEKLRERGDPAAGEVPTVDARAFNCPRCAAPLRIHSPAIESIA
                     CDSCGSIIGVENQDFRLLAGAAQALREIPWLPLGSQGTLRGVNWEVIGFMRRRTTSEG
                     TDYTWSEYLLFNEQQGFAWLIEYQGHWNHARTLANPPSVARGQRDFRRGHQKFKLFNS
                     GQAEVIYVVGEFYWRVAVGETCQVDDFVCPPLLLSREVTAKEANWSQSEYLEPDEVCA
                     AFAVRTPAPKRIGIYANQPNPLLERHRRICRTFWILALAATVLQLAFAFIFASQVVLR
                     QQVVLSSLNEEATLNTQEFVLKERARALLVRHDTDLANNWLSLNITLVEKNTGEAYEG
                     TQEISYYKGVEDDESWSEGSASDEMVFKAVPAGTYYLVIEYELGTDNTVAVADTLEVV
                     RNPAGWSNFVLLLIFLAVFPLLSRWRRSAFEARRWSESD"
BASE COUNT          788 a         1415 c         1391 g          809 t
ORIGIN      
        1 gaactgcggc gttcccggta tcggtttgaa gtggccgaac gacatcctgc tcgacggtgg
       61 aaaactcggt ggcatcctgg ttgaactcga cgcccagccc gacggcatgg tcgcggtgat
      121 gggtatcggc ctgaacctgc tgctgcccgc tgccggcgca gaggaattcc tgcatcggcc
      181 ggcctcgctg tcgcaagcct cctcgccccc gcccgatcgt catcaacttt tggcgcgcat
      241 cctgcttgag ctggcggtcg tcctcgaccg tttcgcggtg gctggtttca gcgtcctgcg
      301 cgaggaatgg caggcgcacc atgtctggca ggagcgcgcc gtgcggctgc tcaacggcgg
      361 gcttcttgac cgcgagggca tctgtctcgg ggccgacagc gacggcgcgc tgctgttgcg
      421 aacggctgcc gggatcgaac gttgtctgtc cggtgacctc tcgctgcgcg cggcatgaac
      481 atcgcgatcg acgccggcaa cagccgtatc aagtggggtg tccatgacgg aaccggctgg
      541 ctcgacagcg gcgttctggc gactgcggat gtcgcctggt tgagcgaggc cgccgacgag
      601 tggccggtgg atgcacgcgt catggtctgc aacgtcgccg gcagcgaggt cgccgcgacc
      661 ctctccgcac tcctcgccgg gcgccacccc agggtctctt tcctgcacgc cagcgccgag
      721 gcttgcggcg tccgcaatac ctacgcgttt ccgggacaac tcggagccga tcgctgggcg
      781 gcactgatcg gtgcgcgcgc gctgcttgag cacgcgtgcc tggtggtttg cgccggcacc
      841 gcgaccacgg tcgacctgct cgatgcggac ggggtgtttc gcggcggctt gatccttccc
      901 ggtttcgacc tgatgcgcgc ggcgctggcc gacaataccg cacagttgcc gctcgccgag
      961 ggtaaatttc gcagcgagcc gcgcaatacc atggacgcca tcgtcagcgg ttgcctgcag
     1021 gcccaactcg gagcggtcga gcgcatgttt gcgaccatcg ccacggcgcc tggcgcgcgt
     1081 tgcctgctga ccggcggcgc cgccgcaccg ctggcggcgc acttgaagat cccgttccag
     1141 ttggcggaca atctgatcct gaacgggctg gtacgctatg ccgaatcgct gtagctggta
     1201 ccattgccga taaacaggag aagtcacatg cccgcgcacc tgatgacgac actgcccgcc
     1261 tttctggcgt atttcgccgt ggcgatggtg ttgctcgccc tgttcctgct ggtctatctc
     1321 aatgtcacgc cgtaccacga gctggccctg attcgtgccg gcaatagcgc cgccgcggtc
     1381 agcctgtcgg gggcgctgct cggttttgcc atgccggtag ccaacgtcat cgcccacagc
     1441 gatacgctga tcgacctggc atcctgggga gcaatcgccg gcatcatcca gattctggcg
     1501 tatctggtcg ccagattcac ccttccacag ctcaatcagg acattcccgc cgacaaactg
     1561 gcgccggcga tctttctggc ggccatttcg ctcggtgtcg gcgtgatcaa cgcggcctgc
     1621 atgggttact gaccgacccc accggcaatc ggtttcgagc gccggatcat ctacgcttgc
     1681 tttagggaga ccaccgtggc cctcatggac ttcatcagaa aacagttcat cgatgtcatt
     1741 cactggactg aagagggcga cggggtgctc gccatgcgct atccgatgcg ggacttcgag
     1801 atccagtacg gcgcgcaact gacggtacgc gagtcgcaga tcgccgtgtt cgtcaacgag
     1861 ggacagatcg cggacgtgtt ccagcccggc cagtaccacc tgacgacgcg gacactgccg
     1921 gtactcacct acctgaagaa ctgggacaag ctcttcgagt cgcccttcaa gtcggacgtc
     1981 tatttcttct cgacgcgcct gcaactcgac tgcaagtggg gaacgccaaa tccgatcacc
     2041 atccgcgaca aggacttcgg gatggtgcgc atgcgtgcct tcggcatcta ttcgtacaag
     2101 ctcgccgacg cacgcaagtt tcaccgcgag atttcgggca ctcgcgagca atacacggtc
     2161 accgacctcg atggtcagtt acgcaacctg gtgatcagct cgatgaccga cctgttcgga
     2221 gaatcgggcg tgccgttcat cgacatggcg gccaaccaga acgaattgag tcgaaccctg
     2281 aaagacaagc tggaggcggt attcgaccgc tacggactgg cgctcgacag ctttgtggtg
     2341 cagaacgtct cgctgccgga agaactccag aagatcctcg atacgcgcat cggcatgaac
     2401 atgatcggcg acctgggccg ctacacgcag tatcaggtgg ccaacagcat tccgctggcg
     2461 gcgcagaacg aaggcggtat tgccggtctt ggcgccggcc tcggcgccgg gctgggcatc
     2521 ggccagacga tgacgacaac ggtggcacag gccctgcaga ccggcgccaa cccaccggta
     2581 acggcggcgg cattggcgcc tgactctgcc ggcagcgccg acgccgtggt cgcgacgctg
     2641 gagaagctgc attccctggt gagcaagggg atcctctcgc aggccgaatt cgacgccaag
     2701 aaggccgagt tgctggccag gttgcagtaa gccgcgtgcc caccagagca gcactgcctt
     2761 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatctggaa cgagtggcac
     2881 atcctgttcg acgacgggcg aggcgcgtgg ctggccgaag ccgccggcga atggattgtc
     2941 agcgcccagg tggcggtcgc cgatcccctg ccggcgttcg aaacgctggc gccggacctg
     3001 ccggtcaccc tcgacggacg cagcttccgg gtggccgatc tcgaaacggc gcgctgcatc
     3061 gccgggcagg gagagttgcc gttcaaggtc gccgccggct acgatgtcaa caccgccgac
     3121 ctgcgcggca acggtcgtta cgtcaccctc gactacagcg agacgccgcc actggtgttc
     3181 gtaggccagg cggtgtcctt cgctgacctg caactggaga aactcaggga acggggcgac
     3241 ccggcagccg gagaggtgcc gacggtcgac gcacgcgcct tcaattgtcc gcgctgtgcc
     3301 gcgccactgc gcattcattc gccagccatc gaaagcatcg cctgtgacag ttgtgggtcg
     3361 atcatcgggg tcgagaacca ggacttcaga ctgctggccg gcgcggcgca ggccctgcgc
     3421 gaaataccct ggctgccact cggcagtcag ggcacgctgc gcggcgtcaa ctgggaagtg
     3481 atcggcttca tgcgccgccg cacgacatcg gaaggcaccg actacacgtg gtcggaatac
     3541 ctgctgttca atgagcagca gggctttgcc tggctgatcg agtaccaggg acactggaac
     3601 catgcgcgca cgctcgccaa cccgccatca gtcgcccgcg ggcagcgcga cttccggcgc
     3661 ggtcatcaga agttcaagct gttcaacagc ggccaggccg aggtcatcta cgtcgtcggc
     3721 gagttctact ggcgggttgc cgtcggagag acctgccagg tcgacgactt tgtctgtccg
     3781 cccctgctgc tctcacgcga ggtcacggcc aaggaagcca actggtcgca atccgaatac
     3841 ctcgaacccg acgaggtctg cgccgctttt gcggtcagga cacccgcacc gaaacgcatc
     3901 gggatctatg ccaaccagcc gaaccccctg ctggagcggc accgtcgcat ctgtcgaacg
     3961 ttctggatcc tggccctggc tgccacggtg ctgcaactgg cgttcgcctt catcttcgcc
     4021 tcgcaggtgg tactcaggca gcaggtggtg ctgtcgtcgc tgaatgagga ggcaacgctg
     4081 aacactcagg aatttgtcct gaaggagcgt gcgcgtgccc tgctggtccg ccacgacacc
     4141 gatctggcca acaactggct gtcgctcaat atcaccctgg tcgaaaaaaa taccggcgaa
     4201 gcctatgaag gtacgcagga aatcagttac tacaaaggtg tcgaggacga cgagagctgg
     4261 tctgaaggat cagcatccga cgagatggtc ttcaaggccg tgccggcggg aacctactac
     4321 ctggtcatcg agtacgaact gggaaccgac aatacggttg ccgtcgccga cacgctggaa
     4381 gtcgttcgca atcccgcggg atggtcgaac ttcgtgctgc tgctgatctt ccttgccgtt
     4441 ttccccctgc tttcgcgttg gcggaggagt gccttcgagg cgcgacgctg gagcgagagc
     4501 gac
//
LOCUS       DAOSIT010000144         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17056_length_4500_cov_9.689989, whole genome shotgun sequence.
ACCESSION   DAOSIT010000144 DAOSIT010000000
VERSION     DAOSIT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17056_length_4500_cov_9.689989"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLL14_04790"
     CDS             complement(<1..1118)
                     /locus_tag="PLL14_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03271.1"
                     /translation="MSSEEKRAGISLAAVFALRMLGLFLILPVFSVYAKDLPSGNDMA
                     LVGIALGAYGLTQACLQIAYGAASDRFGRKPVIVFGLLLFVAGSFVAACADDIYVVIA
                     GRVLQGAGAISAAVTALAADLTREQHLTKVMAMIGSSIGLVFAVSMVGAPLLYVGIGM
                     PGIFALTGVLAMLAIFVVLRVVPAAPAVPRQPAWQSFVEVLSHRQLLRLNFGVFALHL
                     MLTAMWVLLPSELISTGALPVAEHWKVYLPALLLSFVIMLPAIVVGERLGHMKLIFNA
                     AIVLLLAVQAGFGLFAHGLYGLAFWLMLFFVAFNILEATQPSLISRMAPPHAKGAALG
                     VYNTTQALGLFLGGVIGGVLAKYAGAGAVWAGGAALTLL"
     gene            1213..4068
                     /gene="uvrA"
                     /locus_tag="PLL14_04795"
     CDS             1213..4068
                     /gene="uvrA"
                     /locus_tag="PLL14_04795"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG03272.1"
                     /translation="MDETREIRIRGARTHNLKNINLDLPRNRLIVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLQRIEKPDVDLIEGLSPAISIEQKATSHNPRSTVG
                     TVTEIHDYLRLLFARAGTPYCIEHQQPLEAQTVSQMVDHVLALPEESRLMILAPVVAN
                     RKGEQLDLIAELRAQGFARLRVDGQIHDIDAIPKLAKTQKHTVDVVVDRLKVRDDLRQ
                     RLAESFETALRHAEGRAIAYEMDSGREHFFSAKFACPVCSYSLPELEPRIFSFNSPMG
                     ACPTCDGLGVIQFFDPKRIVAHPELSLAAGAIRGWDRRTPFYFQMLCSLAAHYGFEVD
                     TPFRDLPDSVRQIVLYGSGREELPFSYLGETGRMSARTHAFEGIVVNLERRYKETDSQ
                     AVREELARLISNQTCPACQGSRLREEARNVRIGSHASARTLHDVNRQPLTEARDYFLG
                     LQLPGNKAQVAEKVLKEIVSRLQFLINVGLDYLSLDRSAETLSGGEAQRIRLASQIGS
                     GLTGVLYVLDEPSIGLHQRDNERLLKTLKQLRDIGNTVIVVEHDEEAIRSADYVVDIG
                     PGAGVHGGFVVAEGTPAEIADNPASLTGDYLAGRRRIGMHGKRRRPNPERLLRIVGAR
                     GNNLRNVTVDIPVGLLTCVTGVSGSGKSTLINDTLYTAAARHLYGSSAEPAEHDRIVG
                     LDHFDKVVNVDQSPIGRTPRSNPATYTGLLTPIRELFSGVPEARARGYGPGRFSFNVK
                     GGRCEACQGDGVIKVEMHFLPDIYVACDVCHGKRYNRETLEVVYKGRNIHEILQMTVE
                     VAREFFDAVPVVARKLQTLVDVGLCYITLGQSATTLSGGEAQRVKLALELSKRDTGRT
                     LYILDEPTTGLHFQDIEMLLTVLHRLAEHGNTVVVIEHNLDVIKTADWLIDLGPEGGA
                     GGGCILATGTPEQIAAAPASHSGRFLTPLLGQPDAGA"
     gene            4310..>4500
                     /locus_tag="PLL14_04800"
     CDS             4310..>4500
                     /locus_tag="PLL14_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG03273.1"
                     /translation="MNKSEMIDAIAARTELSKAASAKALDAVLETIVDTVTQGEAVTL
                     VGFGSFKASERASREGKNP"
BASE COUNT          931 a         1470 c         1342 g          757 t
ORIGIN      
        1 agcagcgtca gtgccgcacc gccagcccag accgctccgg caccggcata tttggccaga
       61 acgccaccga tgacgccacc gagaaacagt cccagggcct gggttgtgtt gtacaccccg
      121 agggctgcgc ctttggcatg tggcggtgcc atacgcgaaa tcagcgaagg ctgcgtcgct
      181 tcgagaatgt tgaaggcaac gaaaaacagc atcagccaga atgccaggcc atagaggccg
      241 tgcgcaaaca gaccgaaacc agcctggacg gcaagcagga gaacgattgc ggcattgaag
      301 atcagcttca tgtggcccaa gcgctcgccg acaacaatcg ccggcagcat gatgacgaag
      361 gacagcaaca gggcgggcag gtagaccttc cagtgttcgg caaccggcaa ggcgccggtg
      421 ctgatcagtt cgctgggcaa caacacccac atcgcggtca gcatcaggtg cagggcgaac
      481 accccgaaat tgaggcgcag caactgacgg tggctcagga cctcgacaaa gctttgccat
      541 gccggctgcc ggggcacggc gggtgctgct ggcaccaccc tgagcacgac aaaaatggcc
      601 agcatggcca gcaccccggt caatgcgaag attccgggca tgccaatgcc gacatacagc
      661 agcggcgccc cgaccattga aacggcaaag accagaccga tcgacgagcc gatcatggcc
      721 atgaccttgg tgagatgctg ctcgcgggtg agatcggcgg cgagtgccgt gaccgcggca
      781 gaaatggctc ccgcgccctg cagcacccga ccggcaatga cgacgtagat gtcgtcggcg
      841 caggcggcaa cgaaactgcc ggcgacgaac agaagcagcc cgaagacgat caccggcttg
      901 cgtccgaaac ggtcagatgc cgcaccgtag gcaatctgca gacaggcctg ggtcagcccg
      961 taagcgccga gggcgatgcc gaccagcgcc atgtcgttgc ctgaaggcaa gtccttggca
     1021 tagacagaga aaaccggcag gatcagaaac aggccgagca tgcgcagggc aaataccgcc
     1081 gccaggctga tgccggcgcg tttttcttcg ctgctcattc gatcgggatt cgagacggac
     1141 atggagtttg ctgccggacg gcggaattgc gtatattaac aggtttgccg cagcccctca
     1201 ggccagccac ccatggacga aacgagagag ataaggatcc gcggtgcgcg cacccacaac
     1261 ctgaagaaca tcaatctcga cctgccccgc aaccggctga tcgtgatcac cggcctttcc
     1321 ggttcgggca aatcgtcgct ggcgttcgac accctgtacg ccgagggaca gcgccgctac
     1381 gtcgaatccc tgtcggccta tgcccggcag tttctgcaac gcatcgagaa acccgacgtc
     1441 gatctgatcg aggggctgtc gccggcgatt tcgatcgagc agaaggcgac cagtcacaac
     1501 ccgcgctcga ccgtcggcac ggtcaccgaa atccacgact acctgcgcct gctcttcgcg
     1561 cgcgcaggca ccccgtactg catcgaacac cagcagccgc tggaggcgca gaccgtttcg
     1621 cagatggtcg atcacgtcct cgccctgccc gaggaaagtc ggctgatgat cctcgcgccg
     1681 gtggtcgcca atcgcaaggg cgagcagctc gatctgattg ccgagttgcg cgcgcaagga
     1741 tttgccaggc tgcgggtaga cgggcagatc cacgacatcg acgccatccc caagctcgcc
     1801 aaaacgcaga aacacacggt cgacgtcgta gtcgatcggc tgaaggttcg cgacgatctc
     1861 cgtcagcgtc tggccgagtc cttcgaaacg gcgctgcggc acgccgaagg tcgcgccatc
     1921 gcctacgaaa tggacagcgg ccgggagcat tttttttccg ccaaatttgc ctgcccggtc
     1981 tgctcctatt cactgccgga actcgaaccg cggattttct cgttcaacag cccgatgggc
     2041 gcctgcccca cgtgcgacgg ccttggcgtc atccagttct tcgatccgaa gcgcattgtc
     2101 gcgcacccgg agctttccct ggccgccggc gcgatccggg gctgggaccg gcgtaccccc
     2161 ttttacttcc agatgctctg ttcgctggcc gcacattacg gcttcgaagt cgatacgccc
     2221 ttccgtgatc tcccggacag cgtccggcag atcgtgctct acggttccgg gcgcgaggaa
     2281 ttgccgttca gttatctcgg tgaaaccggc cgaatgtcgg cgcgcacgca tgccttcgaa
     2341 ggcatcgtcg tcaatctcga gcggcgctac aaggagaccg actcgcaggc cgtccgcgag
     2401 gaactggccc ggctgatcag caaccagacc tgccccgcct gccagggtag ccgcctgcgc
     2461 gaagaggccc gcaacgtgcg catcggcagc cacgccagcg cgcgcaccct gcacgatgtc
     2521 aatcgccagc cattgaccga agcgcgcgac tatttcctcg gactgcaact gccgggcaac
     2581 aaggcccagg tcgcagaaaa ggtgctcaag gaaatcgtca gccgcctgca gttcctgatc
     2641 aatgtcggtc tcgactacct gtcgctggac cgctcggcgg aaacgctctc cggcggcgag
     2701 gcgcaacgca ttcgcctggc atcacagatc ggctcggggc tgaccggtgt cctgtatgtg
     2761 ctcgacgaac cctcgatcgg cctgcatcag cgcgacaacg agcggctgct gaagaccctc
     2821 aagcagctgc gcgacatcgg caacaccgtg atcgtcgtcg aacacgacga agaagccatc
     2881 cgcagcgccg attacgtggt cgacatcggc cctggcgccg gcgtccatgg cggcttcgtc
     2941 gtcgccgagg gaacgccggc ggagattgcc gacaaccccg cgtcactgac cggcgactac
     3001 ctcgctggcc gccgccggat cggcatgcac ggcaaacgcc gccggcccaa ccccgaacgt
     3061 ctgctgcgga tcgtcggcgc gcgcggcaac aatctcagga acgtcacggt cgacattccg
     3121 gtcggtctgc tgacctgcgt caccggcgtt tccggctcgg gcaagtcgac gctgatcaac
     3181 gataccttgt acacggctgc ggcgcgacac ctctatggct cctccgcgga acccgccgag
     3241 catgaccgga tcgtcgggct cgatcacttc gacaaggtgg tcaatgtcga tcagagcccc
     3301 atcgggcgta ccccccggtc gaatccggca acctacaccg gcttgctgac gccgattcgc
     3361 gaactgtttt cgggcgttcc ggaggcgcgt gcgcgcggct acggccccgg ccgcttttcg
     3421 ttcaacgtca agggagggcg ctgcgaggct tgccagggtg acggcgtcat caaggtcgaa
     3481 atgcactttc tgcccgacat ctacgtcgcc tgtgacgttt gtcacggcaa gcgttacaac
     3541 cgcgagacgc tcgaagtggt gtacaagggc aggaacatcc atgaaattct gcagatgacg
     3601 gtcgaggtcg cgcgggaatt cttcgacgcc gtccccgtgg tggcgcgcaa gttgcagacg
     3661 ctggtcgacg tcggcctgtg ctatatcacc ctcgggcagt cggcgaccac cctctccggt
     3721 ggtgaagcac agcgcgtgaa actggcgctc gaactgtcga agcgcgacac cgggcgaacg
     3781 ctgtacattc tcgacgaacc gaccaccggc ctgcatttcc aggacatcga gatgctgctg
     3841 accgtgctgc atcgtctggc cgagcacggc aacacggtgg tggtcatcga acacaatctc
     3901 gacgtcatca agacggcaga ctggctgatc gacctcggcc ccgaaggcgg agccggcggc
     3961 ggctgtattc tcgccaccgg aacaccggag cagattgccg cggcaccggc gagtcatagc
     4021 gggcgctttc tgacgccgct gctcgggcaa ccggacgcag gcgcctgatc gactccttga
     4081 ttgaacccct tttcagcaaa tccctggcga cgagggaccc caaaaacttg tttggcagcg
     4141 aagaaatcca tgccagaatt accgttttaa gtcatcgtcg ctgtctctgc cttacccctc
     4201 tgccttgccc ctctgcctga cccctctgcc tgaccctttt ccaaacacgc tctgaagtgc
     4261 aagaattcaa ctaagaagaa actttatcca ctccactaca ggagacacca tgaacaagtc
     4321 tgaaatgatc gatgccatcg ctgcccgtac cgaactctcg aaagctgcgt ctgcgaaagc
     4381 ccttgacgcg gtgctcgaaa cgatcgtcga taccgttacc cagggtgaag cggtgacact
     4441 ggtgggtttc ggctccttca aggccagcga gcgcgcgtcg cgtgaaggca agaatccgaa
//
LOCUS       DAOSIT010000145         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17077_length_4496_cov_5.932898, whole genome shotgun sequence.
ACCESSION   DAOSIT010000145 DAOSIT010000000
VERSION     DAOSIT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17077_length_4496_cov_5.932898"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2963)
                     /locus_tag="PLL14_04805"
     CDS             complement(<1..>2963)
                     /locus_tag="PLL14_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03274.1"
                     /translation="SALATLNVVIDDNYPPYVFRDSNGTLIGYLVDMWRLWETKTGVR
                     VDLQGSDWATAQQRMRRRQAEVIDTIFRTEERERSLQFLPAYEAIPVSIYAHVGIGGI
                     IDLTTLRGFLVGVKAGDACIDRLASAGIRSVQQFASYEALVHAAVEGKVRVFCLDDPP
                     ARYLLYLNNAEQLFNQAFTLNTGEFHRAVHKGDAATMELLQRGFASITADEEKALREK
                     WMGVPLALSPYARYLGYALVGGSLVGVLLLLWVMTLRRVVRQRTAKLAATLQAIPDLL
                     FELGLDGRYHDYHSPRTELLVAQPELLIGKLVSDVLPAAAAASCLSALREAHETGYSL
                     GQQFELLLPQGSAWFELSVARKVTGIGEEPRFVVLSRDISARKRAEMRLRMAIEATQL
                     VFWELDLRNGSIRYDASMLQLLGLDQGEDPATSDALMELVHPDHRAELLLRFQEAMRP
                     GDPVFGFEHSIINKAGQCIWLQTRGRVAQRDPAGRALLAVGTAMNISERKQVEQALRE
                     SEALKGSVLASAACGIVATDPKGLITVFNPGAEAIFGYLAEEVVGKVTPTVFHDGDEV
                     AAVARNLSEALGFRVEAGFDALVARARCSGITDERERACVRKDGRHITVRLSVTVMRD
                     AQGVLTGYLGTLVDITAQKQAAAQLDLAAKVFEQGGEGIAITDASGKMVMVNQAFTRI
                     TGYSEAEALGQDPRLLASGRHGAAFYRGMWEAIASLGHWQGEIWNRRKDGSIYPEWLS
                     ISRVERNGLPTHYIGTFIDISQLKEAEASIQRLAHFDPLTGLPNRSLLNERVKHDLSR
                     AHRAREPLALIFLDLDRFKNVNDSLGHRIGDELLVQVAERLTGAVRDEDTVSRLGGDE
                     FILLLPSTDAAGAAHVATKLLEVAAPPYRIGQHELSCTASVGIAMYPVDGESFEALSM
                     CADTAMYRAKQRGRNAYCFFTAEMQERSARTLQLENDLRRALEHGELCLHFQPQFAID
                     DRRLIGAEALLRWQHP"
     assembly_gap    2964..3063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3064..3290)
                     /locus_tag="PLL14_04810"
     CDS             complement(<3064..3290)
                     /locus_tag="PLL14_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03275.1"
                     /translation="MSNESDSGVELDGARAWCHVIYGLHALAVIVGVTGAATVAGAFV
                     FGVPSLLAVVLNYLKRGEVRGTWLDSHYRWQ"
     gene            complement(3379..>4496)
                     /locus_tag="PLL14_04815"
     CDS             complement(3379..>4496)
                     /locus_tag="PLL14_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOG03276.1"
                     /translation="MAPRLRSSAPCQEVVWEGHAVDLSRLPIQLCWPGDVAPLITWGL
                     TVTRGPHKNRQNLGIYRQQVIGPNKVIMRWLAHRGGALDFRDHCLHTPDQAFPVAVAL
                     GADPATMLAAVTPVPDSLSEYQFAGLLRGGKTEVVKCLGSDLQVPAAAEIVLEGFIDP
                     QESALEGPYGDHTGYYNEQARFPVFTIERISMRRNPIYHSTYTGKPPDEPAMLGVALN
                     EVFVPLLQKQFPEIVDFYLPPEGCSYRLATVSIRKQYPGHAKRVMFGIWSFLRQFMYT
                     KLIIVVDDDVDIREWKEVIWAISTRVDAARDVLIAENTPIDYLDFASPVAGLGSKMGL
                     DATNKWPGETSREWGTPIVMDAAVKARVDELWNDLGW"
BASE COUNT          842 a         1458 c         1305 g          791 t
ORIGIN      
        1 ggatgttgcc aacgcaacag cgcctctgcc ccgatcaggc gacggtcatc aatggcaaac
       61 tgcggttgaa aatgcagaca caactcgccg tgttcgagtg cgcgccgcag gtcattctcg
      121 agttgcagcg tgcgcgccga acgctcctgc atctcggcgg taaagaagca ataggcgttg
      181 cgcccccttt gtttcgcgcg atacatcgcc gtatcggcgc acatcgacag cgcctcgaag
      241 ctctcgccgt cgaccgggta catggcaata cccaccgatg ccgtacaact caactcatgt
      301 tgcccgatcc tgtacggcgg cgcggcgact tccagcaatt ttgttgccac atgcgccgcg
      361 ccggcggcat cggtactcgg cagaagaagg atgaactcgt cgcccccgag acgcgacacc
      421 gtatcctcat cgcgaaccgc accggtcagc cgttcggcaa cctgcaccag caattcgtcg
      481 ccaatccggt ggcccaggga atcattgacg ttcttgaagc gatcgaggtc caggaagatc
      541 aacgccagcg gctcacgcgc gcggtgcgcg cggctcagat cgtgtttgac tcgctcgttg
      601 agcaggctgc ggttgggcaa gccggtcagc ggatcgaaat gcgccagccg ctggatgctg
      661 gcttcggcct ccttgagctg cgaaatgtcg atgaaggttc cgatatagtg cgtcggtaat
      721 ccattgcgct cgacccggct gatcgacagc cattccgggt agatgcttcc gtccttgcgg
      781 cggttccaga tttcgccctg ccagtggccc agcgatgcga tggcctccca catgccgcgg
      841 tagaaagccg caccatggcg acccgaggcg agcaggcgcg ggtcctgccc gagcgcttcg
      901 gcttcactgt aacccgtgat ccgggtaaat gcctggttga ccatcaccat cttgccgctg
      961 gcgtcggtaa tggcaatacc ctcgccaccc tgctcgaaga ccttggcggc cagatcaagc
     1021 tgcgccgctg cctgcttttg tgcagtgatg tcaaccaaag tgccaagata gccggtcagc
     1081 acaccctgtg cgtcgcgcat gacggtcacc gacaggcgca ccgtgatatg acgcccatcc
     1141 ttgcgcacgc aggcgcgttc ccgctcatcg gtaatgccgc tgcagcgcgc ccgcgcgacc
     1201 aaggcgtcga aacccgcttc cactcggaaa ccgagcgcct cgctgaggtt tctggccacc
     1261 gctgcgacct cgtcaccatc gtgaaacacg gtcggtgtca ccttgccgac gacttcctcg
     1321 gcgaggtagc cgaagatcgc ctccgcgcca gggttgaaca ccgtgatcag gcccttgggg
     1381 tcggtggcga cgataccgca ggctgcactc gcgagaaccg agcctttcag cgcctcgctc
     1441 tcccgcagcg cctgctcgac ctgcttgcgc tcgctgatgt tcatcgccgt gccgacggcc
     1501 agtagcgcgc gcccggcggg gtcacgctgt gccacccggc cgcgcgtctg cagccagata
     1561 cattgacccg ccttgttgat gatcgaatgc tcgaaaccaa acaccggatc gcccggacgc
     1621 atggcctcct gaaagcgcag cagcaactcg gcacgatgat caggatgtac caattccatc
     1681 agtgcgtccg aagtcgccgg atcctcaccc tgatcgagcc cgagcaactg cagcatgctc
     1741 gcatcgtagc gaatgctgcc gttgcggaga tccagttccc agaacacaag ttgggtggct
     1801 tcgatcgcca tgcgcaagcg catttctgcg cgcttgcgcg cgctgatgtc gcgcgagagg
     1861 acgacaaagc gcggctcttc gccaatcccc gtcactttgc gggccaccga cagttcgaac
     1921 cacgcgctgc cctgcggtag cagcagctcg aactgctggc cgagggagta gccggtttca
     1981 tgagcctcgc gcaaggccga caggcaactg gcggccgctg ccgcaggcaa tacatcggat
     2041 accagcttgc caatcagaag ttctggttga gccaccaaca gttcggtacg cggtgaatgg
     2101 tagtcatggt agcgaccgtc caatcccaac tcgaacagca ggtccggaat ggcctgcagg
     2161 gtcgctgcga gcttggccgt ccgctgcctg acgacgcgcc gcaaggtcat cacccacagc
     2221 agcagcagga caccgaccag tgatcccccg acaagcgcgt agccgagata gcgcgcatag
     2281 ggagacaacg ccagtggcac gcccatccac ttttcccgca atgctttttc ttcgtccgca
     2341 gtaatgctcg cgaagccccg ctgcaacagc tccatcgtcg cggcgtcgcc tttgtgtacc
     2401 gcccggtgga attctccggt attgagcgtg aaggcttgat tgaacagttg ctcggcgttg
     2461 ttcaggtaaa gcaggtagcg cgctggcggg tcgtccagac agaacacccg gacctttccc
     2521 tcaaccgccg cgtgcaccag cgcctcgtaa ctggcaaact gctgcacgct gcggatcccg
     2581 gcgctggcga ggcggtcgat acaggcgtcg ccagccttca cgcccaccag aaaaccccgc
     2641 aaggtggtca ggtcgatgat gccgccgata ccaacatgcg cgtagatcga aaccgggatc
     2701 gcttcatagg ccggcaggaa ctgcaggctg cgctcgcgtt cctcggttcg gaagatggtg
     2761 tcgatcactt cggcctgacg ccggcgcatg cgttgctgtg cggtagccca gtcgctaccc
     2821 tgcaggtcca cgcgcacacc ggtcttggtc tcccacaatc gccacatatc gaccagatag
     2881 ccaatgagcg tgccgttgct gtcgcggaag acgtagggcg gatagttgtc gtcgatgacc
     2941 acattcagag tggccagcgc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnatctgcc agcggtagtg actgtcgagc caggttcccc gcacctcgcc gcgcttgagg
     3121 taattgagaa cgacggccag cagcgagggc acgccaaaaa caaaggcccc ggcaaccgtg
     3181 gcggcaccgg taaccccgac gatcaccgcc agggcatgca agccgtaaat gacgtggcac
     3241 caggcgcgcg cgccatccag ttcgacgccg gagtctgact cgttgctcac ttcaacctcc
     3301 ctgtttgcag accatgcgac aacacatcgc cacgaccggt agcggccacc cagaaacact
     3361 gcgggcaagg cttgtcgctc accagcccag atcattccac agctcatcga cgcgcgcttt
     3421 cactgcggcg tccatgacaa tcggcgtgcc ccactcgcga ctggtttcgc caggccactt
     3481 gttggtggcg tcgagaccca tcttgctgcc gagaccggcg accgggctgg caaagtcgag
     3541 gtagtcgatc ggcgtgtttt cggcgatcag cacgtctctt gccgcatcga cgcgcgtgct
     3601 gatcgcccag atcacttcct tccattcgcg gatgtcgacg tcgtcgtcaa cgacgatgat
     3661 caacttggtg tacatgaact ggcgcaggaa actccagatc ccgaacatca cgcgcttggc
     3721 gtgtcccggg tactgctttc tgatgctgac cgtcgccaga cggtacgaac agccttccgg
     3781 gggcaggtag aagtcgacga tctcgggaaa ctgtttctgg agcagcggca cgaagacttc
     3841 gttgagcgcc acaccgagca tcgccggctc gtccggcggc ttgccggtgt aggtgctgtg
     3901 gtagatcggg ttgcggcgca tgctgatacg ctcgatagtg aacaccggga agcgcgcctg
     3961 ctcgttatag tagccggtat ggtcgccata cggtccttcg agcgcgctct cctgcggatc
     4021 gatgaaaccc tcgagaacga tctcggctgc agccggcacc tgcagatcgg agcccagaca
     4081 cttcacgacc tcggtcttgc cgccacgcag caacccggca aactgatatt cggaaaggct
     4141 gtcgggcacc ggcgtcaccg ccgcgagcat cgtcgccgga tcggccccca gcgccaccgc
     4201 caccgggaag gcctggtccg gcgtatgcag acagtggtca cgaaaatcga gtgcgccacc
     4261 gcgatgcgcg agccagcgca tgatcacctt gttcgggccg atgacctgct gacggtagat
     4321 accgaggttc tggcgattct tgtgcggccc gcgcgtcacc gtcagtcccc aggtaatcag
     4381 cggcgccaca tcgcccggcc agcaaagctg gatcggcagg cgcgacagat cgaccgcgtg
     4441 cccctcccag accacctcct gacacggtgc cgacgagcgc aggcggggcg ccatgt
//
LOCUS       DAOSIT010000146         4493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17094_length_4493_cov_12.087201, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000146 DAOSIT010000000
VERSION     DAOSIT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17094_length_4493_cov_12.087201"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..143
                     /locus_tag="PLL14_04820"
     tRNA            68..143
                     /locus_tag="PLL14_04820"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Trp,seq:cca)
     gene            243..629
                     /gene="secE"
                     /locus_tag="PLL14_04825"
     CDS             243..629
                     /gene="secE"
                     /locus_tag="PLL14_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG03277.1"
                     /translation="MASSQQVDTVNTTADKAKLAASVALVVAALVAYYLLSKQGSIAQ
                     WGGLLVGLGAALVVFFSSEPGKQLVAFGRDAWREVKKVVWPSRKEAIQMTLYVFAFVV
                     VMALFLWLTDKTLEWVFYDLILGWKK"
     gene            629..1207
                     /gene="nusG"
                     /locus_tag="PLL14_04830"
     CDS             629..1207
                     /gene="nusG"
                     /locus_tag="PLL14_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG03278.1"
                     /translation="MTEVVQSPAASPSANPDLKWYVVHAYSGMEKAVERNILERINRS
                     GMQSKFGRILVPTEEVVEIRNGQKKTTERKFFPGYVLVEMVMDDETWHLVKHTNKVTG
                     FVGGAKNRPAPISEEDVQKIVSQMQEGTDKPRHKVEFEVGEYVRVKEGPFTDFNGTVE
                     EVNYEKNKVRVSVTIFGRATPVELEFSQVEKT"
     gene            1315..1746
                     /gene="rplK"
                     /locus_tag="PLL14_04835"
     CDS             1315..1746
                     /gene="rplK"
                     /locus_tag="PLL14_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG03279.1"
                     /translation="MAKKIVGFVKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPLPVVITAFADKSFTFIIKTPPATTLIKKAIKLTKGSAKPNSDKVGVIT
                     RAQLEEIAKTKMKDMTAADLDAAVRTIAGSARSMGVNVEGV"
     gene            1747..2442
                     /gene="rplA"
                     /locus_tag="PLL14_04840"
     CDS             1747..2442
                     /gene="rplA"
                     /locus_tag="PLL14_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG03280.1"
                     /translation="MSKLTKKQKAQQGKVDSNKLYPLADALSIVKECANAKFDESIDV
                     AVQLGVDAKKSDQVVRGAVVLPNGTGKTKRVAVFAQGAKAEEAKAAGADIVGMDDLAA
                     MVKAGDMPFDVVIAAPDAMRVVGTLGQILGPRGLMPNPKVGTVTADVATAVKNAKAGQ
                     VQFRVDKAGIVHSTIGRRSFDADKLKGNLAALIDALTKAKPASSKGVYLRKVAVSSTM
                     GVGVRVDTQSISA"
     gene            2702..3217
                     /gene="rplJ"
                     /locus_tag="PLL14_04845"
     CDS             2702..3217
                     /gene="rplJ"
                     /locus_tag="PLL14_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG03281.1"
                     /translation="MSLNRSEKEAVISDVTGLAAKAQTLVIAEYRGITVADMTKLRNT
                     ARSNGVSLSVLKNTLARRAVAGSAFEVVSDQMTGPLIYGFSEDAVAAAKVVAEFAKTN
                     DKLVIRAGAYGGKALDVNGVKQLASIPTKEVLLAQLLGLMQSPISRTARVLSALAEKK
                     GAGAPAEAAAA"
     gene            3259..3636
                     /gene="rplL"
                     /locus_tag="PLL14_04850"
     CDS             3259..3636
                     /gene="rplL"
                     /locus_tag="PLL14_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOG03282.1"
                     /translation="MAFDKDAFLTALDSMTVMELNDLVKAIEEKFGVSAAAMAAPAAA
                     GGGGAAAAAEEKTEFNVVLLEAGANKVSVIKAVREITGLGLKEAKDVVDGAPKAVKEG
                     LSKADAEAAKKKLEEAGAKVELK"
     gene            3941..>4493
                     /gene="rpoB"
                     /locus_tag="PLL14_04855"
     CDS             3941..>4493
                     /gene="rpoB"
                     /locus_tag="PLL14_04855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871348.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOG03283.1"
                     /translation="MAYSYTERKRIRKSFGSRESVLEVPYLLQMQKDAYTAFLQADAS
                     PRKRTNEGLQAAFEAAFPIVSHNGFVEMKFVEYNLAKPAFDVRECQTRGLTFGSAVRA
                     RVQLIIYDRESSTSQSKVVKEVKEQEVYMGEVPLMTEKGSFIINGTERVIVSQLHRSP
                     GVFFEHDKGKTHSSGKLLFSARII"
BASE COUNT          980 a         1239 c         1392 g          882 t
ORIGIN      
        1 ccatccgcga gggcggtcgt accgtcggcg ccggtgtcgt ggccaagatc cttgcgtaaa
       61 ttatcgtagg ggtatagctc aattggcaga gcgtcggtct ccaaaaccga aggttgtagg
      121 ttcgattcct actgcccctg ccacctgaac cgtggccccc aaagcccgcc agagtcctgg
      181 cgggcttcag cgtctaagag gacgccttga ctcaaagctg aaaagcgcag gatttgccac
      241 aaatggcttc ttcacaacaa gtggacactg tcaacaccac ggccgacaag gccaagcttg
      301 ccgcttctgt ggcactggtc gttgctgccc tcgtcgccta ctaccttctg tccaagcagg
      361 gttccattgc ccagtggggt ggtctgctcg tgggtttggg tgctgccctg gtggtgtttt
      421 tctcgtctga gccaggcaag caactggttg ctttcgggcg cgatgcctgg cgcgaagtca
      481 aaaaggtcgt ctggccctcc cgcaaggagg ccatccagat gacgttgtac gtttttgcct
      541 ttgttgtggt catggcgttg tttctctggc tgactgacaa gacgctcgag tgggtgttct
      601 acgacctgat tctgggatgg aagaaataat gactgaagtt gtccagagtc ctgccgccag
      661 cccttctgcc aacccggacc tgaagtggta tgtcgtccat gcctattcgg gcatggagaa
      721 ggcggtcgaa cgcaacatcc tggagcgcat caaccgctcc ggcatgcaga gcaagtttgg
      781 tcgcatcctg gttccgaccg aggaagtggt cgagatccga aacggccaga aaaagaccac
      841 ggagcgcaag ttctttccgg gttatgtcct ggttgaaatg gtcatggacg acgagacctg
      901 gcatctggtc aagcacacca acaaggtcac cggctttgtc gggggtgcca agaaccggcc
      961 ggccccgatt tccgaggaag acgtccagaa gatcgtgagc cagatgcagg aaggcaccga
     1021 caaacctcgc cacaaggtgg agttcgaagt gggtgaatac gtccgtgtca aagagggacc
     1081 tttcaccgac ttcaatggaa cggtggaaga ggtcaactac gagaagaaca aggttcgcgt
     1141 gtcggtgacg atttttggtc gcgccacgcc ggtcgagctt gaattcagtc aggtcgaaaa
     1201 gacctgatgg actggtctgc gccttgcgac tcggtgcagc tggaagatgg tgagtcgtta
     1261 atctcgggga gcctctgcaa agtctgaggc gtttgcaccc gataggagaa aagaatggcg
     1321 aaaaaaatcg tcggcttcgt caagctgcaa gtgccagcgg gcaaggccaa cccttccccc
     1381 ccgatcggtc cggcgctggg tcagcgcggc ctgaacatca tggagttctg caaggcattc
     1441 aatgcccaga cccagggcgt tgagccgggt ctgcctttgc ctgtggtgat cacggctttt
     1501 gcagacaaga gctttacctt catcatcaag acgccgcctg cgacgaccct gatcaagaaa
     1561 gccatcaagc tgaccaaggg gtctgccaag ccgaactccg acaaggttgg cgtgatcaca
     1621 cgcgcccagc tcgaagagat cgccaagacc aagatgaaag acatgacggc tgcggatctc
     1681 gatgcagcgg ttcggaccat tgcaggttct gcgcgctcga tgggcgtgaa tgtggagggt
     1741 gtctaaatgt ccaagctcac caagaaacaa aaggcccagc aaggcaaggt cgacagcaac
     1801 aagctgtatc cgctggctga tgcgctgagc atcgtcaagg aatgcgccaa cgccaaattc
     1861 gatgaatcga tcgatgttgc cgtgcaactt ggcgtggatg ccaagaagtc ggatcaggtc
     1921 gttcgtggtg ccgtcgttct gccgaatggc accggcaaga ccaagcgagt ggccgtgttc
     1981 gcgcaaggcg caaaggcgga agaagccaag gccgctggtg ccgatatcgt tggcatggac
     2041 gacctggccg ccatggtcaa ggccggtgac atgcccttcg acgtcgtgat tgccgcgccg
     2101 gacgcgatgc gcgtggtcgg tactctgggc cagatcctcg gtccgcgcgg cctgatgccc
     2161 aacccgaagg tgggcacggt gacggccgac gtcgccaccg ccgtgaagaa tgccaaggca
     2221 ggtcaggttc agttccgcgt cgacaaggcc ggcatcgtgc actcgaccat tggacgccgc
     2281 tcattcgatg cggacaagct caagggcaac ctggctgcgc tgatcgatgc gctgaccaaa
     2341 gccaagccgg cttcgagcaa gggtgtgtat ttgcgcaaag ttgcggtctc gtcgaccatg
     2401 ggtgtgggtg ttcgcgtcga cacccagtcc atctcggcgt aatcgcagga attcgggcgg
     2461 cgtgcgagcg ctgcctgatg tggtgggctg caatggccaa tggtcgttgc aggtcatcca
     2521 agaccgttgg cgcgtgggaa accacgctta atcgtagagc ttgatggttc gcctgacggc
     2581 tcagggccaa cgcagatggc gatcccgctg cagatggaat atgagttctg ctgacagttg
     2641 gtcgctgcaa tgtggcgcgg gagatggcat ctgccgtcga acgcaattta aggagtagac
     2701 cttgagtctg aatcgcagtg agaaagaagc ggtcatcagt gatgtgaccg gcctcgccgc
     2761 taaagctcaa acgctcgtga tcgcggagta ccgcggcatc acggttgctg acatgaccaa
     2821 actgcgcaac accgcgcgca gcaatggcgt gagtctgagt gtgttgaaga acaccctggc
     2881 ccgccgtgct gtcgccggaa gcgcgtttga agttgtgtcc gaccagatga ccggtccttt
     2941 gatctacggc ttttccgaag acgcagtggc cgccgccaag gtggtggccg agttcgcgaa
     3001 aaccaacgac aagttggtga tccgggccgg tgcgtacggc ggcaaggccc tggacgtgaa
     3061 cggcgtcaag caattggcca gcatccccac aaaggaagtg ctgttggcgc agttgttggg
     3121 cttgatgcag tcccccattt cccgtacggc ccgcgtgctg tcggctttgg cggagaaaaa
     3181 aggcgcgggt gctcccgccg aagcagctgc cgcgtaaacg ccgcagcctg tcgtaaccaa
     3241 acttgttagg aaatcaaaat ggcattcgat aaagacgcat ttttgaccgc gctggacagc
     3301 atgacggtca tggaactcaa tgacctggta aaggccattg aagagaaatt cggcgtgagc
     3361 gcggcggcga tggctgcccc ggccgccgct ggcggtggcg gcgctgccgc agctgcggaa
     3421 gagaagaccg aattcaacgt cgtcctgctc gaggcaggcg ccaacaaggt gtcggtcatc
     3481 aaggctgtgc gcgaaatcac cggtctgggc ctcaaggaag ccaaggacgt cgtggacggc
     3541 gctcccaagg cggtcaagga aggcctgtcc aaggctgacg ccgaagcggc caagaagaag
     3601 ctggaagaag ccggcgcgaa ggtcgaactc aagtaattgg gatcgatccg aaaggctgga
     3661 ggatcctcag gggcctccag cctttgctgc tttgcagagc acacctgaaa accggcttct
     3721 gggctggttt ccggacgacc cagatgtcga tcggaaacac cagaagagat tttcaagtgt
     3781 tttctgaccc cgactgcaga agacgacttg gttcgggttg cgtgcaatgc gcgaccgtcc
     3841 gccaatggtt ggtagtggcc agccaccaag ctagtcgagc aggttgctcg acgcactagt
     3901 cgccaaagac ccaggacgta tgtctttgac cggagaatcc atggcctatt catacactga
     3961 acgcaagcgc atccgcaaga gttttggcag ccgcgaaagc gtgcttgagg tgccctatct
     4021 gctgcagatg cagaaggatg cctacacggc gtttctgcaa gctgacgcca gccccaggaa
     4081 gcgcaccaac gaagggctgc aggcagcttt cgaggcggcg ttcccgatcg tttcgcacaa
     4141 cggcttcgtg gagatgaagt ttgtcgagta caacctcgcc aagcccgcct tcgacgttcg
     4201 cgaatgccag acccgtggct tgaccttcgg ttcggccgtg cgcgcccgtg ttcagctgat
     4261 catttatgac cgtgaatcct cgacctccca atccaaggtg gtcaaggaag tcaaggagca
     4321 agaggtctac atgggtgaag taccactcat gacggaaaaa ggctctttca tcatcaacgg
     4381 cacggagcgc gtgattgttt cgcagcttca ccggtcgccg ggcgtgttct tcgagcacga
     4441 caagggcaag acccacagct ccggcaaact gttgttctct gcgcggatca tcc
//
LOCUS       DAOSIT010000147         4479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17167_length_4479_cov_6.692586, whole genome shotgun sequence.
ACCESSION   DAOSIT010000147 DAOSIT010000000
VERSION     DAOSIT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17167_length_4479_cov_6.692586"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..594)
                     /locus_tag="PLL14_04860"
     CDS             complement(37..594)
                     /locus_tag="PLL14_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOG03284.1"
                     /translation="MLIGYARVSTQDQNLELQQEALTKAGCQKVFEDKVSGTRADRPG
                     LAKAMEMLREGDALVVWKLDRLGRSVKQLVDLVGELHKQGVQFKSLTDAIDTGTPSGR
                     FFFHVMASLAEMERDLIVERTRAGLDVARQLGRKGGRKPKMTDSKIESAKKLLASGVP
                     PKDVAKNLGVSVPTLYRWVPASAQA"
     gene            complement(588..887)
                     /locus_tag="PLL14_04865"
     CDS             complement(588..887)
                     /locus_tag="PLL14_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03285.1"
                     /translation="MDALPISRGFDDFVDHCVLVGMVHEQEPNDGMPFARSGAVSTAI
                     LIAFLGSNYAERLAQVERHLVDASAKSELLVMMHGGADATQVAHEYIDLGRDKPC"
     gene            complement(907..1293)
                     /locus_tag="PLL14_04870"
     CDS             complement(907..1293)
                     /locus_tag="PLL14_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOG03286.1"
                     /translation="MNESRLSDYIDHIQQAAADARGFVEGVSKDDFLTDKRTQQAVTM
                     SLIIIGEAATKVMDGYAEFTQAHAEVPWRSMRNMRNRMAHGYFDINLDVVWETVQEWL
                     PELLKQLPAVRQGAADKDRNDIMGKS"
     gene            complement(1290..1580)
                     /locus_tag="PLL14_04875"
     CDS             complement(1290..1580)
                     /locus_tag="PLL14_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG03287.1"
                     /translation="MRPSVVLDMKRSAVREAVNRFRTANPRVFGSVLHGTDRDGSDLD
                     LLVDALPGATLLDLGDLEDELKSLLGVHVDLLTPGDLPPKFRAKVLAEAQPV"
     gene            1737..>4479
                     /locus_tag="PLL14_04880"
     CDS             1737..>4479
                     /locus_tag="PLL14_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOG03288.1"
                     /translation="MSRRSILSAADRISLLALPDTEDELIRHYTFSEADLSLIRQRRG
                     DANRLGVAVQLCLLRFPGQGLLPDATVPPLLLQWIGWQLRIDPACWPQYAEREETRRE
                     HLLELRAYLSMEPFRLAHYRQAVHATTELAMQTDKGIVLATSVFDMLRHRYIILPVLD
                     VIERVCAEAITRANRRIYEALSEPLSNEHRDGLDDLLKRRDNGKTTWLASLRQSPAKP
                     NSRHMLEHIERLKARQALDLPTGIERLIHQNRLLKIAREGGQMTPADLTKFEPPRRYA
                     TLVALAIEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINVKVRLYGRIG
                     QALIDARQSGRDPFTAIEAVMSWDAFTESVTEAQKLAQPDDFDFLHRIGENYATLRRY
                     APKFLDVLKLRAAPAAKNVLDAIKVLRGMNTDNARKVPADAPTDFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFASLKQSSELPLAV
                     ATDCDPYLSERLELLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMILPHVKITELLLEVDEWTGFTRHFTHLKSGELAKDKNLLLTTILADAINFGLT
                     KMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQFRHPFAKHWGDSTTSSSDGQ
                     NFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTRPYIPKGEAVYEALKS
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLATALRELGRV
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     "
BASE COUNT          929 a         1449 c         1254 g          847 t
ORIGIN      
        1 gttggggtcg tctcagaaaa cggaaaaaat cgtacgctaa gcctgcgctg aggctggcac
       61 ccagcggtac agcgtaggaa cggacacgcc gaggttcttg gcgacgtcct tgggtggcac
      121 gccgctggcc agcagcttct tggctgactc gatcttgctg tcggtcatct tgggcttgcg
      181 accgcccttg cggccaagct gacgggcgac atccaacccg gcgcgggtgc gctcaacgat
      241 cagatcgcgc tccatttcgg cgaggctggc cataacgtgg aagaagaacc ggccagatgg
      301 cgtgccggta tcaatcgcgt cggtcaggct cttgaactgg acgccttgct tgtgcagctc
      361 gccgaccaga tcgaccagtt gcttgacgct acggcccaag cgatccagct tccagaccac
      421 gagggcgtcg ccctcgcgca gcatttccat cgccttggcc aagccggggc ggtcagccct
      481 cgtaccactt accttgtctt caaaaacctt ctggcatccc gccttggtca aggcttcctg
      541 ctgcaactca aggttctgat cctgcgtcga gacgcgcgca tagccgatca acatggcttg
      601 tctcgcccca ggtcaatata ttcgtgcgcc acctgcgtgg cgtctgctcc gccatgcatc
      661 atgacaagca attcactctt cgcggatgca tccacaaggt gacgctccac ttgtgccagt
      721 ctctccgcgt agttggaccc aagaaaggcg atcagaatcg cggtcgaaac tgctccagac
      781 cgagcgaacg gcataccgtc gttcggttct tgctcatgta ccatccccac cagcacacaa
      841 tgatcgacaa aatcgtcaaa accacgagaa atcggaagtg catccaatag aacttcccgt
      901 tgaccttcac gattttccca tgatgtcatt gcggtcttta tcggcggcgc cctggcgcac
      961 ggcaggcagt tgcttgagca actccggtaa ccattcttgt accgtctccc acaccacatc
     1021 gaggttgatg tcgaaatagc cgtgggccat acgattgcgc atgttgcgca tgctgcgcca
     1081 cggcacttcg gcgtgtgcct gagtgaactc ggcgtagcca tccatcacct tcgtggcggc
     1141 ctcgccgatg atgatgagac tcatggtaac ggcctgctgg gtgcgcttat cggtaaggaa
     1201 gtcgtccttc gacacccctt ccacgaagcc gcgcgcatcc gctgcggcct gctgtatgtg
     1261 atcgatgtaa tcagaaagac ggctctcatt cataccggtt gcgcctctgc aagtaccttt
     1321 gctcgaaact ttggcggcag gtcgcctggt gttagcaagt caacgtgaac gccgagcagc
     1381 gatttcaatt cgtcttctaa atcgcccaag tccaataacg tggcaccagg cagcgcatcg
     1441 accaacagat caaggtcgct gccgtcccgg tcggtgccat gcagcaccga accgaagacg
     1501 cgcggattcg ccgtgcgaaa acggtttacc gcttcgcgca ctgcacttcg cttcatgtcg
     1561 agcacaacgg acggtcgcat gggcattctt tcttatcgaa actcattgca atggtaatgg
     1621 atcgagaata acgtttcaag aaggattttc gagaatcgca acgccttgat ttcgcgtgcc
     1681 gcgcagaatg gccgggcagg cttgtcacaa acctacgttt tcaagaagga gactgcatgt
     1741 cccgtcgttc gatcctgtcc gcagccgacc gaatcagcct gctggcatta cccgacaccg
     1801 aagacgaact catccgacac tacaccttca gcgaggccga tctgtcgctg attcggcagc
     1861 gccgtggaga tgcgaaccgg ctcggcgtcg ccgtgcagtt gtgcttgctg cgctttcccg
     1921 gtcaggggct gctgcctgat gccactgtgc cgccgctgct gttgcaatgg atcggatggc
     1981 aactgcgcat cgatccggcg tgctggccgc aatatgcgga acgtgaggaa acccggcgtg
     2041 agcatctgct cgaactgcgt gcgtacctga gcatggagcc gttcaggctg gcgcactacc
     2101 ggcaggccgt ccacgccacc accgaattgg ccatgcagac agacaagggc atcgtactgg
     2161 ctaccagcgt atttgacatg ctgcgccacc gatacatcat tctcccggtg ctggatgtca
     2221 tcgagcgcgt ctgcgctgaa gccatcaccc gcgccaaccg gcgtatctat gaagccctgt
     2281 ccgagccgtt gtcgaacgag caccgggatg gcctcgacga tttgctgaaa cgccgtgaca
     2341 acggcaagac gacctggctg gcctcgctgc gccagtcacc ggccaaaccg aattcgcgcc
     2401 acatgctcga acatatcgaa cgtctcaaag ctcggcaggc actcgacctg ccgaccggca
     2461 tcgagcggct cattcatcag aaccgtctcc tgaaaatcgc ccgcgagggt ggccagatga
     2521 cgcccgctga cttgaccaag ttcgagccac cgcggcgcta cgccaccctc gttgccttgg
     2581 ccatcgaggg catggccact gtcaccgacg agattatcga cctgcacgac cgtatcctgg
     2641 gcaagctatt caacgccgcc aagaataagc atcagcagca atttcaggca tcaggcaaag
     2701 ccatcaacgt caaggtgcgt ctgtacgggc gcatcggcca ggcgctgatc gacgccaggc
     2761 aatcaggccg cgacccgttc actgccattg aggccgtcat gtcctgggac gccttcaccg
     2821 agagtgtcac cgaggcgcag aagctcgctc agcccgatga cttcgatttc ctgcaccgca
     2881 tcggcgagaa ctacgccacc ttgcgccgct acgcaccgaa attcctcgac gtgctcaagc
     2941 tgcgggcggc gcccgccgca aagaacgtgc tcgacgccat caaggtactg cgcggcatga
     3001 acaccgataa tgcccgcaag gtgcctgccg atgccccgac cgacttcatc aagccacgct
     3061 ggcagaagct ggtgatgacc gacgccggca tcgaccgccg ctactacgaa ctgtgtgcgc
     3121 tgtcggagct gaagaactcg ttgcgctcgg gcgacatctg ggtgcagggg tcgcgccagt
     3181 tcaaggactt cgaggactac ctagtgccgc ctgagaagtt cgccagcctc aagcagtcca
     3241 gcgaattgcc gctggccgtg gccaccgatt gcgatccgta cctgagcgag cgcctggaac
     3301 tgctggaggc gcagcttgcc accgtcaatc gcatggcggc ggccaacgac ctacccgacg
     3361 ccatcatcac cgagtcgggc ctgaagatca cgccgctgga tgcggcggtg cccgacaccg
     3421 cgcaggcgct gatcgaccag acggccatga tcctgccaca cgtcaagatc accgaactgc
     3481 tgctcgaagt cgatgagtgg acaggcttca cccggcactt cacgcacctg aaatcaggcg
     3541 agctggccaa ggacaagaac ctgttgctga ccacgatcct ggccgacgcg atcaattttg
     3601 gtttgaccaa gatggccgag tcctgccccg gcacgaccta cgccaagctc tcctggctgc
     3661 aagcctggca tatccgcgac gaaacctatt cgacggcgct ggccgaactg gtgaatgccc
     3721 aattccggca tccctttgcc aaacactggg gcgacagcac cacgtcatcg tcggacggtc
     3781 agaacttccg aaccggcagc aaggccgaga gcaccggcca catcaacccg aaatatggca
     3841 gcagcccagg gcggactttc tacacccata tctctgacca gtacgcgccc ttccacacca
     3901 aggtggtcaa tgtcggcctg cgcgactcga cctatgtgct cgacggcctg ctgtaccacg
     3961 agtctgacct gcgtatcgag gagcactaca ccgacacggc gggcttcacc gatcacgtct
     4021 ttgcactgat gcacctactg ggcttccgct ttgcgccgcg catccgcgac ttgggcgaca
     4081 ccagacccta catcccgaaa ggcgaagccg tctatgaggc gctgaaatcg atgatcggtg
     4141 gcacgctcaa catcaagcat gtccgcgccc attgggatga aatcctgcgg ctggccacgt
     4201 caatcaagca aggcacagtg acggcctcgc tgatgttgcg aaaactcggc agttacccgc
     4261 gccagaacgg tctggccacg gccctgcgcg agctggggcg cgtcgaacgt acgctgttca
     4321 tcctggactg gctgcaaagc gtggaactgc gccgccgcgt gcatgccggg ttgaacaagg
     4381 gcgaagcccg caacgcccta gctcgtgccg tgttcttcaa ccggctgggc gaaatccgcg
     4441 accgcagttt cgagcagcag cgctaccggg ccagcggcc
//
LOCUS       DAOSIT010000148         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17274_length_4454_cov_6.548988, whole genome shotgun sequence.
ACCESSION   DAOSIT010000148 DAOSIT010000000
VERSION     DAOSIT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17274_length_4454_cov_6.548988"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1340)
                     /locus_tag="PLL14_04885"
     CDS             complement(207..1340)
                     /locus_tag="PLL14_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG03289.1"
                     /translation="MANTRTALVLGGGGARAAYQVGVLLAIRELLGESAANPFPILCG
                     TSAGAINAATLACHAHNFGAAVEALADVWRNMHAGQIYRADPLGISLAGGRWLGALLF
                     GWAVGHSPRSLLDNRPLRQLLEAKLNFRHIARAITKGSLYAVSITASGYATGHGVSFF
                     QAHPEVQRWRRTQRVGCRTQLSVEHLLASSAIPFFFPAVHLNREYFGDGSMRQLAPIS
                     PAIHLGAEKVLIIGVGQMRDSGERESSRRYPTLAQIAGHALSSIFLDSLAVDVERMER
                     INRTLAKVPDELRASGELALRPVQSLIITPSERLDYLAAGHAGALPWAVRTLLRGLGA
                     MNRRGGALTSYLLFEPSYTGALMDLGYQDTMARRQDVRAFLES"
     gene            complement(1433..>1924)
                     /locus_tag="PLL14_04890"
     CDS             complement(1433..>1924)
                     /locus_tag="PLL14_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG03290.1"
                     /translation="TYINYEPSAEQLVDMTLLAADEVRRFGLTPRVALLSHSSFGAEN
                     TPTALKMREALRLLKERAPRLEVEGEMHGDAAIDDHIRQRVFPSDARLKGQANLLIMP
                     TLDAANISFNLLKIASGDNLTIGPILLGAAQPVHILTSTATVRRIVNMTALTVVDKLM
                     SER"
     assembly_gap    1925..2024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2025..3468)
                     /locus_tag="PLL14_04895"
     CDS             complement(<2025..3468)
                     /locus_tag="PLL14_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOG03291.1"
                     /translation="MNKAKDQLRTAALFYHRNPKPGKVSIQPTKQLTNQYDLSMAYSP
                     GVAAACEEIVAFPAEASTLTARANLVGVITNGTAVLGLGAIGPLAAKPVMEGKAVLFK
                     KFANIDVFDLEIEERDPDRLVEIVASLEPTFGGINLEDIKAPECFYVEKKLRERMKIP
                     VFHDDQHGTAIVVGAAILNGLFLQGKELDQVKVVTSGAGAAALACLDLLVMLGIPVEN
                     IWVTDIKGLVYEGRIEDMDEIKGRYAKQTDARTLGEVIEGADVFLGLSAGGVLKPEMV
                     ARMAPSPLIMALANPNPEIKPEDVKAVRSDAIIATGRSDYPNQVNNVLCFPFIFRGAL
                     DVGATTITEAMKLAAVRAISELARVEQSDAIVKAYGEKVANFGPDYLIPNPFDPRLIG
                     KIAPAVAAAAMASGVATRPIKDIDVYRESLDEFVYHFGLIMKPIFSQAKQAPRRIVFA
                     EGEDERVLRAVQVIVDEGIARPILIGQPDEI"
     gene            3830..>4454
                     /locus_tag="PLL14_04900"
     CDS             3830..>4454
                     /locus_tag="PLL14_04900"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765364.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOG03292.1"
                     /translation="MNQTVSINAPDYVKHERLKQWVADIAALTEPADVVWADGSQDEY
                     DRLCGELVAAGTFVKLAKRKNSFLAFSDPSDVARVEDRTYICSEKKENAGPTNNWEDP
                     AKMRELLGGLFKGCMKGRTMYVIPFSMGPIGSPIAQIGIEITDSAYVVVNMRIMTRMG
                     KDVFPVLGTDGVFVPCVHSIGAPKEPGQTDPRWPCNPTTKYIVHYPET"
BASE COUNT          858 a         1391 c         1267 g          838 t
ORIGIN      
        1 tctctgccgc ctgcacagac tccatcggga tcaacatcgt acctaccaca gtacccaaca
       61 acaccgctgc tctcagcgtg attcgcattg caagcctcct gcctgattcg attggcgaca
      121 ttctacagca gtccgttgcc ggggcatcat aaatcatgaa aaataagggc atagacacgt
      181 tatatcatgt accacatcat gcttcttcag gattcgagaa aggcacggac gtcctgccga
      241 cgggccatgg tgtcctgata accgagatcc atcaacgccc cggtatagga cggttcgaag
      301 agcaggtaac tggtgagagc accaccccgg cgattcatcg cccccagacc acgcagcagc
      361 gttctcaccg cccacggcaa ggcgccggca tgacctgcag ccaggtaatc gagccgctcg
      421 gacggggtga tgatcagcga ttgcaccggc cgcaatgcaa gctcgccact cgcccgcaat
      481 tcatccggca ctttcgcaag ggtgcggttg atacgttcca tgcgctccac gtcaaccgcc
      541 aggctatcga gaaagatcga cgagagcgcg tgccccgcga tctgcgcgag ggtcggatag
      601 cggcgcgaac tttcccgctc tccggagtca cgcatttgcc cgacgccgat gatcagcact
      661 ttctcggctc ccaagtggat cgctggcgaa attggcgcca actggcgcat tgagccgtcg
      721 ccgaaatatt cgcgattgag gtgcacggcg ggaaagaaaa agggaatcgc gcttgaggcc
      781 agcaggtgtt cgaccgacaa ttgcgttcgg cagccgacgc gttgcgtgcg ccgccacctc
      841 tgcacctcgg gatgtgcctg aaaaaaactg acgccatgcc cggtggcgta gcccgatgcg
      901 gtgatgctta ccgcgtagag tgagcctttg gtgatggcgc gggcgatatg gcgaaaattc
      961 agcttcgctt cgagaagctg ccgcaatggc cggttatcga gcagggaacg tggactgtgc
     1021 cccaccgccc agccgaacag cagggcgccc agccagcgcc cgccggcaag gctgatgcca
     1081 agcggatcgg cccgataaat ctgcccggcg tgcatgttgc gccagacatc cgccagcgct
     1141 tcgacggcgg cgccaaaatt gtgggcatgg catgccagcg ttgcggcgtt gatggcgccg
     1201 gccgaggtac cgcacagaat cggaaacgga ttggcagcgg actcgccaag caattcgcgg
     1261 atggccagca agacgccgac ctggtacgcg gcacgcgcgc cgccgccgcc aagaaccagg
     1321 gcagtcctgg tgttcgccat cccgcgccgt ggctatcgga atggcgaagc gaatgccgta
     1381 gcagccattg tcggtccacc ggccgcaccc gccgcttgtc ggagcacagc catcagcgct
     1441 cgctcatcag cttgtcgacc accgtcaggg cggtcatgtt cacgatccgt cgaacggtcg
     1501 ccgtcgacgt caggatatgc acgggttgcg ccgcgccgag caggatcggg ccaatcgtca
     1561 gattatctcc tgaggcaatc ttcaacagat tgaaggatat gtttgccgca tccagcgtcg
     1621 gcatgatcag caggttggcc tgccccttga ggcgcgcgtc gctgggaaat accctttgcc
     1681 gaatgtgatc gtcgatggcc gcatcgccgt gcatttcgcc ctccacctca aggcgcggtg
     1741 cacgttcctt cagcagtcgc aaagcctcgc gcatcttcag cgcggtcggc gtattctcgg
     1801 caccgaacga ggagtgcgac agcagggcga cgcgcggagt caaaccgaac cgccgaacct
     1861 cgtcggcggc cagcagcgtc atatccacca attgctccgc cgacggttcg tagttgatat
     1921 aggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaattt catcgggctg
     2041 gccgatcagg atcggtctgg caatcccttc gtcgacgatc acctgtacgg cacgcagcac
     2101 acgttcgtct tcaccttccg cgaagacgat gcgtctggga gcctgcttgg cctgcgagaa
     2161 gatcggtttc atgatcaggc caaagtgata gacgaactcg tcgaggctct cgcgatagac
     2221 gtcgatgtcc ttgattggcc tggtcgcgac tccggatgcc atcgctgccg cggcgaccgc
     2281 tggcgcaatc ttgccgatca ggcgcggatc gaaggggttc ggaatgagat aatccggacc
     2341 gaaattggcg accttctcgc cgtacgcctt gactatcgcg tccgactgct cgacacgcgc
     2401 cagttccgag atcgcccgga cggcggccag tttcatcgct tcggtgatgg tcgtagcccc
     2461 gacgtcaagc gcaccacgaa agatgaaggg aaaacagagc acgttgttga cctggttcgg
     2521 atagtccgag cggccagtgg cgatgatcgc atcgctccgt accgccttga cgtcttctgg
     2581 cttgatctcc gggttgggat tggccaacgc catgatcagt ggcgaaggcg ccatccgcgc
     2641 caccatctcc ggtttgagaa ccccacctgc agacagcccg agaaagacgt cggcaccctc
     2701 gatgacttcg cccaatgtac gtgcgtcggt ctgtttggcg tagcgtccct tgatctcgtc
     2761 catgtcctcg atgcgaccct catagaccag ccccttgatg tcggtcaccc agatgttctc
     2821 gacggggata cccagcatca ccagcaggtc caggcaagcc agtgcggcgg cgccggcacc
     2881 cgaggtcacc accttgacct gatcgagctc cttgccctgc aggaacaggc cattgagaat
     2941 cgccgcgccg acgacgatcg ccgtcccatg ctggtcgtca tggaagacag gaatcttcat
     3001 ccgttcgcgc agtttcttct cgacgtagaa gcattccggc gccttgatgt cttcgaggtt
     3061 gatgccgcca aaggtcggtt ccagcgaagc cacgatctcc accaggcgat cggggtcgcg
     3121 ttcctcgatt tcaaggtcga acacgtcgat attggcaaac ttcttgaaca gtaccgcctt
     3181 gccctccatc accggtttgg ctgccagcgg gccgatcgca ccgagaccaa gcactgccgt
     3241 accattggtg atgacgccga ccagattggc gcgcgcagtc agcgtactgg cttcagccgg
     3301 gaaggccaca atctcttcgc aggccgccgc gacgcctggc gagtaggcca tcgacaagtc
     3361 gtactggttg gtcagctgct tggttggctg tatcgacacc ttacccggtt tgggattgcg
     3421 gtgatagaac agggcggcag ttcgcaattg atctttggct ttgttcatgg cttactctcc
     3481 tgacagaata tcgagccaaa gcaggtgctt gcggctcgcc gacggccaac caacgaccgc
     3541 ttccgaggtc tcggcgcagg cccttcggtg gcgctgggct tgcgcatcga tcgttggtgc
     3601 aggtcggacc tgtggcataa ttacacaaac cttgacttcc ttgtagcagc accgcctcgc
     3661 atgccgacct caccccagca ctgggcttgt ttctccacag ccgcgaccga cgcgtcacca
     3721 ccgacggggc cggatcgacg attccggctc ccggcttctc tggcaagact ctgcgcgcgc
     3781 cgcgccacgg taacttctgc ttctcttcct tccttcgaga gtgcttacca tgaaccagac
     3841 cgtttccatc aatgcccctg attacgtcaa acatgaacgc ctgaaacaat gggttgccga
     3901 tatcgctgcg ctcaccgagc cagccgatgt cgtctgggcc gacggttctc aggacgagta
     3961 tgaccgcctt tgcggcgagt tggtcgccgc cggcaccttc gtcaagctcg ccaagcgcaa
     4021 gaactcgttc ctggcttttt ccgacccgtc cgacgtggcg cgcgtcgaag accgcaccta
     4081 catctgctcc gagaagaagg aaaacgccgg tccgaccaac aactgggaag acccggccaa
     4141 gatgcgcgaa ctcctcggcg gcctgttcaa gggctgcatg aagggccgca ccatgtacgt
     4201 gattcctttc tcgatgggcc cgatcgggtc gccgatcgcg cagatcggca tcgagatcac
     4261 cgacagcgcc tacgtggtgg tgaacatgcg catcatgacc cgcatgggca aggatgtgtt
     4321 cccggtcctc ggtaccgacg gcgtgttcgt cccctgcgtg cattcgatcg gcgcgccgaa
     4381 ggagcccggt cagacagacc cgcgctggcc gtgcaacccg accaccaagt acatcgtgca
     4441 ctatcccgaa accc
//
LOCUS       DAOSIT010000149         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17439_length_4420_cov_6.863459, whole genome shotgun sequence.
ACCESSION   DAOSIT010000149 DAOSIT010000000
VERSION     DAOSIT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17439_length_4420_cov_6.863459"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..304
                     /locus_tag="PLL14_04905"
     CDS             98..304
                     /locus_tag="PLL14_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03293.1"
                     /translation="MKGFVHDDHEAKPAAEMPKLPVSKSKKADSHHHGSHDHGTNKRI
                     IDLIVAVDDAAGMMCPALTMGLCQ"
     gene            301..1287
                     /locus_tag="PLL14_04910"
     CDS             301..1287
                     /locus_tag="PLL14_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOG03294.1"
                     /translation="MKQISFSEAEFAAKRPVTRRERFLADRERVIPWQDVLAIIEPYY
                     PKGKRGRPPIGLERMLRVYLVQQCYGLSDEGVEDAITDSQALRGFVRIDLSREAVPDA
                     TTLLQFRHLLEEKDLSKAIFAAINAQLTAKGLMMREGTIADATILAAPPSVKNEAKAR
                     DPEMHQTKKGNQWHFGMKAHIGVDAESGLVHTVVGTAANAADVTQTAEVLHGEETTVY
                     LDAGYTGVEKREELKDRDIDWQVATKRSKLKAIPKESQLGPLLRRLESVKASIRSKVE
                     HPFHIVKNLFSHRKVRYKGLKKNTAQLHILFALANLVIAKRQLLALHSQDAS"
     gene            1549..1989
                     /locus_tag="PLL14_04915"
     CDS             1549..1989
                     /locus_tag="PLL14_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOG03295.1"
                     /translation="MRNHVLVLALITAFGAGLSSVATAGEMVDVYKNPNCDCCGKWID
                     HLKDAGFEVRAHNVMDVPATRKRLGMPDRLGSCHTAKVAGYVVEGHVPAGDIQRLLKE
                     KPKALGIAVPSMPPGSPGMESSKPVPYNTLLVQSGGEASIFAKH"
     gene            2008..2352
                     /locus_tag="PLL14_04920"
     CDS             2008..2352
                     /locus_tag="PLL14_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HOG03296.1"
                     /translation="MKTLITASLMAIASLGAVSVQAAGNHAGHAMAAPAAASAEMQMV
                     DGQVKKVDKAAGKVTLSHGPLTNLNMPAMTMVFKVSNAAWLDQMKSGDKIRFMADTVK
                     GAITVVHFEPTK"
     gene            2431..3702
                     /locus_tag="PLL14_04925"
     CDS             2431..3702
                     /locus_tag="PLL14_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG03297.1"
                     /translation="MRRLRPLSILILALATASGAPVLAQEAALGANVDTLINYAKTRN
                     PEYAAMQAEAEASGERVTPAGALPDPKFRTELRDITRMGEQSATLSPSRVGSTRYLLM
                     QDIPWFGKRDLKREIAELEADGAKGRALGTWADVAGRIKVNFAQLYYVHRNEQLTREI
                     LDLMTRLEKVAQVRYSGGLAAQQDVIRAQVEQTNMRNELIALETEQHHLHARLNALLA
                     RPNNAPLQVPTQLRKLPAPVTLDYATLEERVRTRNPQLFADESKIKAAEKSRDLTYKN
                     RYPDFTLGVSPIQYQNSIKEWELMFEVNIPLQQSSRRSQERESESMLNAARSRKEATT
                     NQVSAELAEALSGIEAARRTENLLTNSLLPQAELTFKAALAGYETGKVDFATLLDSQR
                     QIRQARQDQLKAQVDAQMRLAEIERLLGEDL"
     gene            3699..>4420
                     /locus_tag="PLL14_04930"
     CDS             3699..>4420
                     /locus_tag="PLL14_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG03298.1"
                     /translation="MNKGAGLTVGVIAVAMAAGSGYWLGGRGDASHGDAASVASASAE
                     PAKKEKKLLFYRNPMGLPDTSPVPKKDPMGMDYIAVYEGEQDDEPASANQIKISTEKV
                     QKLGVRTEAAQLRVLDKVVRAAGRIEPDERRIYAISPKFEGYVERLHVNVTGQPVGKG
                     QPLFEVYSPELVSAQREYAIAAQGVESLKEAGGQARDGMKQLADSSLLRLKNWDISEE
                     QVKALAKSGEARRTLTFRSPVSG"
BASE COUNT          979 a         1335 c         1349 g          757 t
ORIGIN      
        1 gtgacatcgg gttaatcgtg tcggactcat ttcgtccaac cgcgatgcca tcttatgagt
       61 ccgataccaa caggacgctg acctacgcgt tacgtttttg aaaggtttcg tacatgacga
      121 ccacgaagca aagccggccg ccgagatgcc gaaactgccg gtgagcaaga gcaaaaaggc
      181 cgacagtcac caccatggca gccatgacca tgggacgaac aagagaatca ttgacttaat
      241 cgtcgcggtg gacgatgccg ctggtatgat gtgcccggcg ctgacgatgg gattgtgcca
      301 gtgaagcaga tcagcttttc ggaagccgag tttgccgcga agaggccggt aaccagacgc
      361 gagcgctttc ttgccgacag ggaacgggtc atcccctggc aagacgtgct ggcgatcatc
      421 gaaccgtact acccgaaagg gaagcgcggc cggccgccga tcgggctgga gcgcatgctg
      481 cgggtgtacc tggtccagca atgctacggc ctgtcggacg agggggtaga ggatgcgatc
      541 accgacagcc aggcgttgcg gggattcgtg cggatcgatc tgtcgcgaga ggcggtgccg
      601 gatgcgacga cgctgctgca gtttcgccac ttgctggaag agaaggacct gtcgaaggcc
      661 atcttcgcgg cgatcaacgc gcaactgacg gccaaggggc tgatgatgcg cgagggaacg
      721 attgccgatg cgacgatcct tgccgcaccg ccgtcggtga agaacgaagc gaaggcccgc
      781 gatccggaga tgcaccagac gaagaagggc aaccagtggc acttcggcat gaaagcgcac
      841 atcggggtcg atgccgagag cgggctggtg catacggtgg tcggaacggc ggcgaacgcc
      901 gccgacgtga cgcagaccgc cgaagtgctg cacggcgaag agacgaccgt gtatctggat
      961 gccggttaca ccggtgtcga gaagcgcgaa gagttgaaag accgggacat cgactggcag
     1021 gtggcgacca agcgcagcaa gctgaaggcg atcccgaagg agagccagct tggcccactg
     1081 ctgcggcgcc tggaatcggt caaggcgagc atccgctcga aggtcgagca cccctttcac
     1141 atcgtgaaga acctcttcag ccatcgcaag gtccgctaca agggactgaa gaagaacacg
     1201 gcccagttgc acatcctctt tgcgctggcc aatctggtca tcgccaagcg acagcttctg
     1261 gcccttcaca gccaagatgc gtcctgagca cggcgaaggg ccccgaaaag gagcggcaaa
     1321 ggccaggacg aggctttgaa ccccggagaa atcggccggt acggcgcgac gcctgaccgc
     1381 gagcgctcgg tttgccgact tggaggggcc tttgcgcgtg ttgaggtcca ttgatcagcg
     1441 gttccctagg cggcccaaca ttacacaaat gtaatcagcg ggacagctaa ttgttggcat
     1501 tgcctatcca tactgctgac gccactcgat tcaaccaggg gcctcgccat gcgtaaccac
     1561 gtccttgttc tcgccttgat cacagccttc ggtgccggcc tttcctccgt cgcaacggca
     1621 ggcgaaatgg tcgatgtcta caagaacccg aactgcgatt gctgcggcaa gtggattgac
     1681 cacctgaagg acgcgggctt cgaggttcgc gcgcacaatg tcatggacgt cccggcaacc
     1741 cggaagcgac tggggatgcc agaccgcctc ggctcctgcc ataccgccaa ggtagccggt
     1801 tatgtggtcg agggccatgt gcctgctggc gacatccagc gcctgctcaa ggaaaagccg
     1861 aaagcccttg gcatcgcggt gccttcgatg ccccccggtt cgccaggcat ggaaagttcg
     1921 aaacccgttc cctacaacac gctgctggtc cagtccggcg gtgaagccag catctttgcc
     1981 aaacattgat cccaatagga gaaacccatg aaaacactca tcaccgcctc cctgatggcc
     2041 attgcatctc tcggagccgt ttcggtccag gctgcaggca accacgccgg ccatgccatg
     2101 gctgcaccgg ccgccgcgtc cgctgaaatg caaatggtcg acggccaggt caaaaaagtg
     2161 gacaaggccg ctggcaaggt gaccttgtcg cacggcccgt tgaccaacct gaacatgccg
     2221 gcgatgacca tggtcttcaa ggtttcaaat gccgcctggt tggaccaaat gaagtccggc
     2281 gataagattc gcttcatggc cgacaccgtg aaaggtgcca tcaccgtcgt ccatttcgaa
     2341 ccaaccaaat agcaagatcg actggcggcg agagggtgat tccatctcgc tgccagcatg
     2401 ctccacaaaa tgaatatttc aggaggtcgc atgcgccgac tcaggccgtt atccattttg
     2461 atcctggcgc ttgccaccgc ctctggcgcg cccgttcttg cccaggaggc cgccctcggt
     2521 gcgaacgtcg acaccctgat caattacgcc aagacgcgca atccggagta cgccgcgatg
     2581 caggccgagg ccgaagcatc gggcgagcgg gtgacgccag ctggtgcctt gcccgacccg
     2641 aaattccgta ctgaattgcg cgacatcacc cggatgggcg aacaaagcgc aacgctttcc
     2701 ccaagtcgcg tcggcagcac ccgttacctg ctgatgcagg acattccctg gtttggcaag
     2761 cgcgacctga agcgcgagat tgcagagctt gaagccgacg gagccaaggg tcgtgcgctg
     2821 ggcacctggg cagatgtggc cgggcggatc aaagtcaatt tcgcccagct ttattacgtg
     2881 caccgcaatg agcaattgac ccgggaaatc ctcgacctga tgacccgcct ggaaaaagtc
     2941 gcccaggtac gctattccgg cggcctggcg gcacagcagg atgtcatccg agcccaggtc
     3001 gagcagacca acatgcgcaa cgagttgatt gccttggaaa ccgaacagca ccacctgcat
     3061 gcccggctga acgccttgct ggcccgccca aacaatgcgc cgttgcaggt gccaacccag
     3121 ttgcgcaagc tgccggcgcc ggtgacgctg gattacgcca cgctggaaga gcgcgtgcgc
     3181 acccgcaacc cgcaactgtt cgccgacgaa tcgaaaatca aggctgccga gaaatctcgc
     3241 gacctgacct acaaaaaccg ctatcccgac tttacgctgg gcgtatcccc catccagtac
     3301 cagaactcca tcaaggaatg ggagttgatg ttcgaagtga atatcccgct gcaacagtct
     3361 tcgcggcggt cgcaggagcg ggaatccgag tcgatgctca atgccgcccg ctcgcgcaag
     3421 gaagccacca ccaaccaggt ctccgccgaa ctggccgagg ccctgtccgg catcgaagcc
     3481 gcccgtcgca cggaaaacct gctgacaaac agtctgctgc cgcaggccga attgaccttc
     3541 aaggcggcac tggccggcta cgagaccggc aaggtggatt tcgccacgtt gctagattcc
     3601 cagcgccaaa tccggcaggc cagacaagac cagcttaagg ctcaagtcga cgcccagatg
     3661 cgtctggccg aaatcgaacg tcttttaggg gaagatctat gaacaagggt gcgggactga
     3721 ccgtcggcgt tattgcggtt gccatggcag ccggtagtgg ttactggctg ggcggacgtg
     3781 gtgacgcttc ccatggcgat gcagcatcgg tcgccagcgc ctcagccgag ccggccaaga
     3841 aagagaaaaa gctgctcttc taccgcaatc cgatggggct gcccgacact tcgcctgtgc
     3901 ccaagaaaga tccgatgggg atggattaca tcgcggtcta tgagggagag caggatgacg
     3961 aaccggcatc tgccaaccag atcaagatca gtaccgagaa ggtgcagaaa ctgggtgtgc
     4021 ggacggaagc cgcccaattg cgcgtccttg acaaggtggt ccgcgccgcc ggtcgtatcg
     4081 aaccggacga gcgccgtatc tatgcgatct ctcccaagtt cgagggctac gtcgagcgcc
     4141 tgcacgtcaa tgtcaccggc cagccggtcg gcaaggggca gccgctgttc gaggtctaca
     4201 gcccggaact ggtttccgcc cagcgcgaat acgcgattgc cgcccaaggc gtcgaatcgc
     4261 tgaaggaagc cggcggccag gcgcgggatg gcatgaagca actggccgat tccagcctgt
     4321 tacgcctgaa gaactgggat atttcggagg agcaggtcaa ggccttggcc aagtccggcg
     4381 aagcgcggcg cacgctgact ttccgctcgc cggtttccgg
//
LOCUS       DAOSIT010000150         4414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17468_length_4414_cov_6.267951, whole genome shotgun sequence.
ACCESSION   DAOSIT010000150 DAOSIT010000000
VERSION     DAOSIT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17468_length_4414_cov_6.267951"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..454
                     /locus_tag="PLL14_04935"
     CDS             2..454
                     /locus_tag="PLL14_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOG03299.1"
                     /translation="MHIDWSSFTPGEAVSGGIRIGIGSVVLVLWNGRIAGISGILGGL
                     VSRVDGDVAWRLAFIVGLSAAPWLWAATHHGSLPKGSLAPASPGEWAQVVVGGLLVGF
                     GTRLAKGCTSGHGVCGLARFSPRSLLAVVAFMGAGILTVFFTRHLAGL"
     gene            456..905
                     /locus_tag="PLL14_04940"
     CDS             456..905
                     /locus_tag="PLL14_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003289368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03300.1"
                     /translation="MTCLTAFIGGILFGLGLIVAGMNDPLKVLAFLDVAGRWDPSLAL
                     VMMGAISTAAAGFAFAARRRCTLLGEALRLPDRRQVDAPLLVGSVLFGVGWGLAGYCP
                     GPGIVGLWAGSLPATVFVIAMLFGIEAHAWYDQSRWQTEVGEVVADS"
     gene            complement(1099..2958)
                     /locus_tag="PLL14_04945"
     CDS             complement(1099..2958)
                     /locus_tag="PLL14_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HOG03301.1"
                     /translation="MNIPTPTAKPQAGGWTAPTGLPGLPDEATLARLAGVFFRALPGS
                     PVPASTPVPVPSPTFPAASGLSSGEPVLPALQASPAPPASPPGTEGDWRAPELGVPES
                     YAAALPTVVPISSPAGVPGSPYYFLSEATAQTSAQQLPASPPGATENHIVAHPFELPG
                     DVALRRVLGELAGERTEQPARAPHPSTGDSRFYFIDLPSAIPNQADASRFPAFDVHAV
                     RRDFPILGERVNGKPLVWFDNAATTQKPQAVIDRLVQFYQHENSNIHRAAHELAARST
                     DAYEAARGKVARFLGASSAEEIIFVRGATEAINLVAKTWGRQNIGEGDEIVVSLLEHH
                     ANIVPWQQLAKESGAHIRVIPVDDKGQILLEEYQHLLNDRTRLVAISQVSNALGTVTP
                     VKEIIDLAHRAGARVLVDGAQSVSHMRVNVQALDADFFVFSGHKVFAPTGIGVVYGKR
                     DLLETMPPWQGGGNMIADVTFERTLYQGVPARFEAGTGNIADAAGLGAAIDYVQRIGI
                     ENIARYEHDLLTYATQRVSPIPGIRLIGTADDKASVLSFILAGYRTEEVGSALNEEGI
                     AVRAGHHCAQPILRRFGVEATVRPSLAFYNTCEEVDLLVSVLQRLAKGKGRTV"
     gene            complement(2933..3859)
                     /locus_tag="PLL14_04950"
     CDS             complement(2933..3859)
                     /locus_tag="PLL14_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOG03302.1"
                     /translation="MSDTSPQAALSDLAARQLANATKTVPQLSTISPRWLVHLLQWLP
                     VEAGIYRLNQVKNPRDVRVACAKRDESVLPQTFVDYEDKPREYFLGAVTTILDVHTRV
                     SDLYSSPHDQIKEQLRLTIETIKERQESELINNPDYGLLANVADSQRISTLTGAPTPD
                     DLDDLLTKVWKEPAFFLTHPLAIAAFGRECTRRGVPPPTVSLFGSQFVTWRGIPLIPS
                     DKVAVEEGKTKVLLLRVGDKRQGVVGLYQPGLPGEQSPGLSVRFMGIDRNAIASYLIS
                     LYCSLAVHAEDALAVLDEVEVDKYHEYPDTYR"
     gene            complement(3919..4212)
                     /locus_tag="PLL14_04955"
     CDS             complement(3919..4212)
                     /locus_tag="PLL14_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG03303.1"
                     /translation="MSRLSDRFPGVVRQMREAQGWSQEHLAESADLNRSYVGEIERGI
                     ASPSLLTAEKLAQALGIKLSELVAHCENHEHPPLSSGSHSDGYSMLSCRKRKA"
     gene            complement(4209..>4414)
                     /locus_tag="PLL14_04960"
     CDS             complement(4209..>4414)
                     /locus_tag="PLL14_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOG03304.1"
                     /translation="ALEKGTARHPIVEDDVVIYAGATILGRVTIGRGSSIGGNVWLTH
                     SVPPGSVLTQAVSRNEVLASGGP"
BASE COUNT          744 a         1322 c         1513 g          835 t
ORIGIN      
        1 catgcacata gactggagca gctttacacc cggggaagcg gtgtctggcg gcatccggat
       61 tggcatcggc agcgtcgtgt tggttctgtg gaacggccgt attgccggca tcagcggcat
      121 cctcggcggc ctggtgagtc gggttgatgg agacgttgcc tggcgtctcg cgttcatcgt
      181 cggattgtcg gcggccccct ggctatgggc ggcgacacac cacggcagcc tgccaaaagg
      241 cagtctagcg ccggcatcgc cgggagaatg ggcgcaggtg gtagttggcg gactgctggt
      301 cggctttggg acgcgtctcg ccaaggggtg caccagcggc cacggcgtat gtggactggc
      361 acgcttctcg ccgcgctcgc tgctggcggt cgtcgccttc atgggcgcag gaatattgac
      421 cgttttcttc acccgccacc ttgccggact gtgaaatgac ctgtctcact gcctttattg
      481 gcggaatcct gttcggactc ggcctgattg tcgccgggat gaacgatccc ttgaaggtgc
      541 tagccttcct cgatgtggcc gggcgttggg atccctcgtt ggctctggtg atgatgggtg
      601 cgatatcgac cgcggctgcc ggtttcgcct tcgcggccag gcgccggtgc accttgctgg
      661 gcgaggctct gcgattgccc gaccggcggc aagtcgatgc gccgctgctc gtcggcagtg
      721 tgctgttcgg cgtcggctgg ggattggccg gctactgtcc ggggccgggt atcgtcggcc
      781 tgtgggccgg ctcgctgccc gcgacggtct tcgtcattgc catgctgttc ggcatcgagg
      841 cacacgcctg gtacgaccag tcacgctggc agaccgaagt cggcgaggtt gttgccgata
      901 gctagcgtag ccggagcgcc gaacgcaccc catcgcgggc agcaagacga tcgagagtgg
      961 cagggtggtg cgtgcgtgcg ggacacgcag ctcgtcggcc agcatagcgg ctgccgggcg
     1021 accgccaacc gctcggatac aaaaaccccc ggcaacgccg ggggaacttg ttgccagaga
     1081 aactatcggc ctgcctggct aaactgtccg acccttaccc ttcgccagtc gttgcagcac
     1141 cgagaccagc agatccactt cctcacacgt gttgtaaaac gccagcgagg gccgcaccgt
     1201 ggcttcgacg ccaaagcgac gcagaatcgg ttgcgcgcag tggtggccag cgcgcaccgc
     1261 gatgccttcc tcgttcagcg ccgagcccac ttcttccgta cggtagccgg caaggatgaa
     1321 ggaaaggacg ctcgccttgt cgtcggctgt gccgatcagg cggatcccgg gtatcggact
     1381 gacgcgctgc gtggcgtagg tgaggagatc atgctcatag cgggcaatat tctcgatgcc
     1441 gatcctttgc acgtagtcga tcgcggcgcc gagtccggcg gcatcggcga tattgccggt
     1501 gccggcctcg aaacgcgccg gcacgccctg gtacagggtg cgctcgaagg tgacgtcggc
     1561 aatcatgttg cctcctccct gccagggcgg catggtttcc aggaggtcgc gcttgccata
     1621 aaccacgccg atgccggtgg gagcaaacac cttgtggccg gagaacacaa agaagtcggc
     1681 atccagagct tgcacgttga cccgcatgtg cgaaacggac tgggccccat cgaccaacac
     1741 cctggcgccg gcgcggtgag cgaggtcgat gatttccttc accggagtga cggtacccag
     1801 agcattggaa acctgactga tagccaccag tcgagtgcgg tcgttgagca ggtgctgata
     1861 ttcctccagc agaatctggc ccttgtcgtc gaccgggatg acccggatat gggcaccgct
     1921 ttccttcgcg agttgttgcc agggcacgat gttggcgtgg tgctccagca gcgaaacgac
     1981 gatttcatca ccttcgccga tgttctgcct gccccaggtc ttggctacca ggttgatggc
     2041 ttcggtggcg ccacgcacga aaatgatctc ctcggccgaa ctcgcaccga ggaagcgggc
     2101 gaccttgccg cgcgcggctt cgtaggcatc ggtcgagcgc gcggcgagtt cgtgggcggc
     2161 gcggtggatg ttggagtttt catgctggta gaactgcacc aaccggtcga tgaccgcctg
     2221 cggcttttgc gtggtggcgg cattgtcgaa ccagacgagc ggtttgccgt tgacgcgttc
     2281 gccgagaatc gggaagtcgc ggcgcacggc atggacgtcg aaagccggga aacggctggc
     2341 gtcggcctgg ttgggaatcg ccgacggcag gtcgatgaag tagaacctgc tgtcgccagt
     2401 cgacgggtgt ggcgcacggg ctggctgctc ggtgcgctcg ccagccagct cgcccagcac
     2461 ccggcgcagc gcgacgtcac ccggcaattc gaaagggtgc gccacgatat ggttttctgt
     2521 tgctccggga ggcgacgccg gcagttgctg ggcagatgtc tgagccgttg cctcggacag
     2581 aaagtagtag ggcgacccag gcacaccggc tggcgaactg atcggcacga cggtcgggag
     2641 ggccgccgca taggattcgg gaactcctaa ctccggcgcc cgccagtcac cttccgtacc
     2701 tggcgggctg gccggaggcg ctggcgaagc ctgtagcgcc ggtagcactg gttcgccgct
     2761 ggacaaaccg ctcgctgccg ggaaagttgg agaagggacg ggcactggcg tggacgccgg
     2821 caccgggctc cccggcaggg cccggaaaaa aacgccggcg agccgggcaa gcgtggcttc
     2881 gtccggcagc ccgggaaggc cagtgggcgc tgtccagccg cctgcctgcg gcttagcggt
     2941 aggtgtcggg atattcatgg tacttgtcga cttccacttc atcgagaacg gccagcgcgt
     3001 cttcggcatg cacggccagc gagcagtaga gcgaaatcag gtaggaagca atggcattgc
     3061 ggtcgattcc catgaagcgc accgacaggc cggggctctg ttcgccgggc aggccaggct
     3121 ggtagagacc gacgacacct tggcgcttgt ccccgacgcg cagcagcagg accttggtct
     3181 tgccttcctc gaccgccacc ttgtccgaag ggatcagcgg aatcccgcgc caggtgacga
     3241 actgcgaacc gaagagcgaa acggtgggcg gtggcacgcc gcggcgggtg cactcgcgac
     3301 caaaggcggc gatcgccagc ggatgggtca ggaaaaaggc cggctccttc cagaccttgg
     3361 tcaacaggtc gtcgaggtcg tccggcgttg gtgcgccggt gagggtcgaa attcgctggc
     3421 tatcggcaac attggccagc aggccgtaat cggggttgtt gatcagttcg ctttcctggc
     3481 gctccttgat cgtctcgatg gtcagccgga gctgttcctt gatctgatcg tgcgggctgc
     3541 tgtacagatc ggaaacacgg gtgtgcacgt cgaggatggt ggtcacggcg ccgaggaagt
     3601 actcgcgcgg cttgtcctcg taatcaacga acgtttgtgg cagcaccgac tcgtcgcgct
     3661 tggcgcaggc gacgcggacg tctcgggggt tcttgacctg gttcaggcgg tagatgcccg
     3721 cctccaccgg caaccactgc agcagatgca ccagccaacg cggcgagatg gtcgaaagct
     3781 gcggaacggt cttggtggcg ttggccagtt gtcgtgcagc gagatcgctc agggcggcct
     3841 ggggggatgt gtctgacatg gggtactcct tactcctcgc gagtgttgct gggcttagaa
     3901 aaaaaacgaa taacgatatt acgctttgcg cttacggcag ctcaacatgc tatagccgtc
     3961 tgaatggctg ccgctgctca ggggtgggtg ctcgtgattt tcgcaatgcg cgaccagttc
     4021 cgagagtttt atccccagcg cctgcgccag tttctcggcg gtgagcaacg acgggctggc
     4081 gatgccacgt tcgatttcgc cgacgtagga gcggttgagg tcggcacttt ccgcgaggtg
     4141 ttcctgcgac cagccctgtg cctcgcgcat ctgccggacg acgcctggga agcgatcgct
     4201 gagcctgctc atgggccacc gctggccagc acttcgtttc gggagacggc ctgggtgaga
     4261 acgctgcctg gcggaacgct gtgggtcagc cagacgttgc cgccgatcga cgagccacga
     4321 ccgatggtga cgcggccgag aatggtcgcc ccggcgtaga tcaccacgtc gtcctcgacg
     4381 atcgggtggc gcgccgtgcc tttttccagg gcac
//
LOCUS       DAOSIT010000151         4400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17552_length_4400_cov_10.555581, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000151 DAOSIT010000000
VERSION     DAOSIT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17552_length_4400_cov_10.555581"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLL14_04965"
     CDS             complement(<1..1183)
                     /locus_tag="PLL14_04965"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HOG03305.1"
                     /translation="MNEPELAALIARQSPEQVVDALLALPLEARRALAPIAAELLKAV
                     DSGRLLNPWTGLLPGLPTTRTGNLGRRWNEHHAKLALAVLTLCPIDKARRVRWLGLHD
                     ADALLRRVFVARDPAWRDAWLRHRLRDEFPGVEWSFVRGLVRDGLCARPEEDGYVRLM
                     VSGLNPGWSRKGESHVPVSARLLADPGLLEDEVWRLFATDTAAFSDAWQRNNPRRPEN
                     YESWSDALLRLADAGLLDRQRLLDASLSGLWKSTSSNVLSGYHQLHQRLAPTAAELDA
                     REAQYRDLLSHRTGHVVGFALRQLASIARRRPLAAAPTLTSMLPLFSLPAKAHALAAL
                     KLVGRIMQRAGQEGREAAITDAAHAVLFEALRHRESEVQEAAAQLLAAGADAWDAAQV
                     SQ"
     gene            complement(1180..2382)
                     /locus_tag="PLL14_04970"
     CDS             complement(1180..2382)
                     /locus_tag="PLL14_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HOG03306.1"
                     /translation="MLLALLDVVRSHYFLPRPPQMDPVVTSNEAMLRFEGFSGCCGVY
                     VRVDLDTSGLQAERLGRGTTNVDFNLPMRAALMRLQDHERVEFSVGREAFALSHGAER
                     VVEKKVSLPLRWIKGFSEVQAYQPRLLLMAEVTGVEARHFARSLPLGSVPRQPSFAVQ
                     SGGSLRLSQRAADGAVRISGLQRLRTLPGLVQRAERLRIWSDATAGASAWEVIFPVGR
                     VTLLVSPEAQRGFSGEGQVLGTLAGNAWQQALPRVRAELRWQNEIDVDRVAAATRLSV
                     AEVEGALAALGARGLAGYDRATGRYFHRELPFDLDRVEALQPRLLDARELLTGSKVRL
                     LQTIDAGGHDFAVDGSEVVHHVRLRAGQDRCSCPWFSKLQGERGPCKHILAARLFLDG
                     AADVPISS"
     gene            2873..3841
                     /locus_tag="PLL14_04975"
     CDS             2873..3841
                     /locus_tag="PLL14_04975"
                     /inference="COORDINATES: protein motif:HMM:NF020432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HOG03307.1"
                     /translation="MRALTMMLSGFLAIGLAGTAEAKGSKHDGDSAIPECGKNTKVAR
                     PDEEGIITLKEKKKRGCVIDVKALRPTQSAVGMDAVECKAEKISSNARKGKLNDYLLA
                     DNRWVPMVRGPGGVFYLTDHHHLSTAVWNADIKDKEKKVYAFLLADWSNMKEDAFWQQ
                     MVTQHDTWLKAPDGKDITPAQLPRSIGALQDDPLRTLSAWVRGSCGYVKCDPPGAAKD
                     DDDPSCADKFPNVTCADAYFLEFKWGAYLANVQEVKDALAGEASCSQQTPLNTSCLDS
                     QYDKLTKALPAAMQAAASPAAQQAVGEGAGYNPAVQTGIAQPKHCK"
     gene            complement(3927..>4400)
                     /locus_tag="PLL14_04980"
     CDS             complement(3927..>4400)
                     /locus_tag="PLL14_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG03308.1"
                     /translation="HQAWSNKGYSLLKLQRPAEAVPAFETALRLKPDFSNAWINLGEA
                     KLAQRQAGEAIVALRKALELSPGASDARLYLASAYVSTGQFALAREQLTPLAEKAPQL
                     VQAWVLLTVANAGLGDRDAATASYTRLKSLNPAAAKELRTRLAALPASRAVTLPE"
BASE COUNT          766 a         1511 c         1465 g          658 t
ORIGIN      
        1 attgcgagac ctgagcagcg tcccaggcgt cggcaccggc cgccaggagt tgcgccgcgg
       61 cctcctggac ctcgctctcg cgatgccgca aggcctcgaa caataccgcg tgcgcggcgt
      121 cggtgatcgc tgcctcgcgc ccctcctggc cggcccgctg catgatcctg ccgaccagct
      181 tcagcgcggc cagggcgtgc gccttggccg gcaggctgaa cagcggcagc atgctggtca
      241 acgtcggtgc tgccgccagc ggccggcggc gggcgatcga cgccagttgc cggagggcaa
      301 agccaacgac atggccggtg cgatggctga gcaggtcccg gtactgcgct tcgcgcgcat
      361 cgagttctgc agccgtcggc gccagccgct gatgcagctg gtgatagccg gaaaggacgt
      421 tgctgctggt gcttttccac aatccggaca ggctcgcgtc gagcaggcgc tggcggtcga
      481 gcaggcccgc gtcggcgagg cgcagcaagg cgtcactcca gctttcgtag ttctcgggtc
      541 gccgcgggtt gttgcgctgc caggcgtcgc tgaaagcggc ggtatcggtg gcgaacagcc
      601 gccagacttc gtcttcgagc agaccggggt cggccagcag tcgggcactc accggtacat
      661 ggctctcgcc cttgcgcgac catcctggat tgagtccgct gaccatcagc cgcacgtagc
      721 cgtcttcctc ggggcgcgcg cagagtccgt cgcgcaccag gccgcggacg aaactccact
      781 cgacgccggg aaattcgtcg cgcaaccggt gacgcagcca ggcgtcgcgc caggccgggt
      841 cgcgggcgac gaatacccgc cgcagcagcg cgtccgcgtc gtgcaggccg agccagcgca
      901 cacgccgcgc cttgtcgatc ggacacagcg tgagcaccgc caacgcgagc ttggcgtggt
      961 gctcgttcca gcggcgtccg agattgccgg tgcgcgtcgt cggcaaaccc ggcagcagcc
     1021 ccgtccatgg gttcagcagg cgtccgctgt cgaccgcctt caacagttcg gcggcaatcg
     1081 gtgccagcgc gcggcgagcc tccagcggca aggccagcag cgcgtcgacc acctgctcgg
     1141 gcgactgtct ggcgatcagc gccgccaact ccggctcgtt catgatgaaa tcggcacgtc
     1201 ggcagcgcca tcgaggaaca gccgcgccgc caggatatgc ttgcagggac cacgctcgcc
     1261 ctgcagtttg ctgaaccacg ggcaggagca gcgatcctgc ccggcgcgca ggcgcacatg
     1321 gtggaccacc tcgctgccat cgaccgcgaa atcatggccg ccggcgtcga tcgtctgcag
     1381 cagccgcacc ttgctcccgg tcagcagttc gcgggcatcg agcaggcgcg gctgcaaggc
     1441 ctcgacgcgg tcaaggtcga acggcagttc tcgatgaaag tagcggccgg tcgcccggtc
     1501 gtagcccgcg aggccgcgcg cgccgagcgc ggcgagcgcg ccctcgacct cggcgacgct
     1561 cagccgggtg gcggcggcga cgcggtcgac gtcgatctcg ttctgccagc gcaactcggc
     1621 gcggacccgc ggcaacgcct gctgccaggc gttgccggcg agcgtgccga gcacctgacc
     1681 ttcgcccgag aagccccgct gcgcctcggg gctgaccagc agggtcaccc gcccgaccgg
     1741 aaagatcacc tcccaggccg aagcgcccgc tgtcgcgtcg ctccagatgc gcagccgttc
     1801 ggcccgctgc acgagccccg gcaaagtccg caggcgttgc aggccgctga tccgcaccgc
     1861 gccgtcggcc gctcgctgac tcagacgcag gctgccgccg gactggacgg cgaacgacgg
     1921 ctgcctgggg acgctgccca acggcaggct gcgggcgaag tggcgagcct ccacacccgt
     1981 cacctcggcc atcagcagca gccgcggctg atacgcctgc acctcgctga agcccttgat
     2041 ccagcgcagc ggcaggctga ccttcttctc gacgacgcgt tctgcgccgt gactcagggc
     2101 gaacgcttcc ctgccgacgg aaaactcgac ccgctcgtgg tcctgcaaac gcatcagcgc
     2161 ggcccgcatc ggcaggttga aatcgacgtt ggtggtgccg cggcccagcc gctcggcttg
     2221 cagccctgag gtatcgagat cgacgcgcac atagacccca cagcagccgc tgaacccctc
     2281 gaaacgcagc atcgcctcgt tgctggtcac caccgggtcc atctgcggtg gccgcggcag
     2341 aaagtagtgg ctgcgcacga catcgagcag cgccagcagc atgtcggcaa cgctgcgcgg
     2401 ctgttcgatg cgcccctgaa agaacggcgg gtgtgcgtgg cccgacgaac aggtcgccag
     2461 ccgcagcgac tgcgcgcccg ccgagccgac gacgcccgac gcgtagggat aacggtaggt
     2521 atagttcaag gcacttgccg acatgctgtt gatcgctccg cgaaatgact cagtgctgcg
     2581 gcctcagtgc tgcggaccca aacggttgcg gacccagggc tgcgcaatgc cggtgtttcc
     2641 tggtacccgc ggcacgcgcc gctgcggtga cccggcgccg cgcccgccat tctcgcagtt
     2701 tttcttgccg gccccgccgc ggcacaccct gcggcggcaa gacgatcgat atcttttccg
     2761 caatcagaat gagattgact tcgcctgcga cgccatcgat actgcgcgcg ggcgactgaa
     2821 tgcccaggag gatctgttct gtttctcact ggtttaacaa aggagggaag agatgcgcgc
     2881 actcacgatg atgctttcgg gatttctggc gatcggcctg gcgggcactg ccgaggccaa
     2941 aggcagcaaa catgacgggg actcggcgat ccccgaatgc ggcaagaata ccaaggtcgc
     3001 ccggcccgac gaggagggaa tcatcaccct caaggaaaag aagaagcgcg gctgcgtcat
     3061 cgacgtgaag gcgctgcgtc cgacgcaatc ggcggtggga atggacgccg tggagtgcaa
     3121 ggcggaaaag atatcgagca acgccaggaa gggaaaactc aacgactatc tgctggcaga
     3181 caatcgctgg gtgccgatgg tgcgcggccc gggcggcgtg ttctacctga ccgaccacca
     3241 ccacctgtcc accgccgtct ggaatgccga tatcaaggac aaggagaaaa aggtctatgc
     3301 cttcctgctc gccgactggt cgaacatgaa ggaggatgcg ttctggcagc agatggtcac
     3361 gcagcacgac acctggctga aggctcccga cggcaaggac atcacccccg cgcaactgcc
     3421 caggagcatc ggcgccttgc aggatgatcc gctgcggacg ctctcggcct gggtgagggg
     3481 gagctgtggc tacgtcaagt gcgatcctcc cggggccgcc aaggacgatg acgacccgag
     3541 ctgcgccgac aagtttccga acgtgacctg cgccgacgct tatttcctcg agttcaagtg
     3601 gggcgcctac ctggccaacg tgcaggaggt caaggatgcg ctggccggag aagcctcctg
     3661 ctcgcagcaa acgcccttga atacgtcgtg cctcgacagc cagtacgaca aactgaccaa
     3721 ggccctgccg gcggcaatgc aggcggcggc ttcacccgcg gcgcaacagg cggtaggcga
     3781 aggcgccggc tacaacccgg ccgtgcagac cggcatcgcg caacccaagc actgcaagta
     3841 agcccgaggc cagagggcgg agccaactgc gtacagccgc aggcctgccg cgactccacc
     3901 gctccgtaag gtggcggcgg taaatgctac tcaggcagag tcaccgccct gctcgccggc
     3961 aacgccgcca ggcgggtgcg caactccttc gccgccgcgg gattcagcga cttcagccgg
     4021 gtatagctgg cagtggccgc gtcgcggtcg ccgagcccgg cgttggcaac ggtcagcaac
     4081 acccatgcct gcaccagttg cggcgctttc tcggccagcg gtgtcagttg ctcccgggcc
     4141 agcgcgaact ggccggtgct gacgtacgcc gaggcgagat acagccgcgc atcgctggcc
     4201 cccggcgaaa gttcgagcgc cttgcgcaag gcgacgatcg cctcgccggc ctggcgctgc
     4261 gcgagtttgg cctcgccgag gttgatccag gcgttgctga aatcgggctt gaggcgcaac
     4321 gccgtttcga acgccggcac cgcttccgcg gggcgctgca gcttgagcag actgtaaccc
     4381 ttgttgctcc aggcctgatg
//
LOCUS       DAOSIT010000152         4387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17612_length_4387_cov_6.120729, whole genome shotgun sequence.
ACCESSION   DAOSIT010000152 DAOSIT010000000
VERSION     DAOSIT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17612_length_4387_cov_6.120729"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..309)
                     /locus_tag="PLL14_04985"
     CDS             complement(88..309)
                     /locus_tag="PLL14_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG03309.1"
                     /translation="MNGLNERFPSIVRQLRHERGWSQERLAERANLNRSYVGEIERGL
                     ASPSLVTADKLARALEVDLSALVARCEHG"
     gene            complement(306..1271)
                     /locus_tag="PLL14_04990"
     CDS             complement(306..1271)
                     /locus_tag="PLL14_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOG03310.1"
                     /translation="MSAPLRSTPDTSTSDLWGLEDIVRDLRAAREKWRTAQQRPLESG
                     GREFPSRAALRDVVERLCGALFPMRLGPPDLQQGHSEDFYVGHTLDAALRNLLDQVRL
                     ELTHASRHQPEVDPTTIETQSIAVVQHFAAALPGIRVLLDSDVEAAFRGDPAARSVDE
                     IVLCYPGTLAIIHHRLAHTLYALGVPLLARIVSELAHSATGIDIHPGARIGNSFFIDH
                     GTGVVIGETAVIGNRVRLYQAVTLGAKRFSVDVDGALEKGTARHPIVEDDVVIYAGAT
                     ILGRVTIGRGSSIGGNVWLTHSIPPGSFLTQAVSRNEMLESGGGI"
     gene            complement(1286..1807)
                     /locus_tag="PLL14_04995"
     CDS             complement(1286..1807)
                     /locus_tag="PLL14_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG03311.1"
                     /translation="MLATAQPDAASPTTEYRPPDAAAQPTDLHHILAAASARAALGNL
                     PYAGTVAPREAWALAAAGAAILIDVRTAEEYKYVGHVPGTPLVQWQSGAALTRNPRFT
                     RELAGKASKNDAILLICRSGKRSAAAAEAATRAGFSRVFNVLEGFEGEITPDGQRGAT
                     GGWRYHGLPWVQD"
     gene            complement(2086..2904)
                     /locus_tag="PLL14_05000"
     CDS             complement(2086..2904)
                     /locus_tag="PLL14_05000"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017701595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03312.1"
                     /translation="MSQQLLDLTVTAKSFADLPVLRNVHFRINTGEVVSLVGPRGCGK
                     STLLRIIAALDSCFAGEVLINGQPPSLRSRDVGLIFQEPRLLPWLTVAENVGFALGIK
                     GRGHPLVAALLSEMGLVNCAGAYPRQLSGDMAQRVAIARGLCSQPSLLLLDEPFSAVD
                     AFTRKHLQDLLLSIASRRRTTLLLVTHEVSEAVYFSDRVIVLASQPGRISGEVVIGAP
                     RPRDRRNPQLARKEAKVLTLLSDGTTNSFDLSYALASAPPTWPPVQAAHLHFAI"
     gene            complement(2918..3751)
                     /locus_tag="PLL14_05005"
     CDS             complement(2918..3751)
                     /locus_tag="PLL14_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016850165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG03313.1"
                     /translation="MSQTGLSRAPALLARNRPRAYRRWLRYSHGLWIPAILMLIWQTL
                     SGSGWVSARLLPSPSELLGTLAELVENGALLTHAGISTLRLLTGFSIGAVLGILAGTA
                     AGLSQRVETLFGPTFRTLRTVPLLAWVPLALLWLGSGEATKITLIAIGAFFPVYLNVV
                     AGIHDVDDKLLEVGGLFGLSGSLLITRVFLPAALPNLFSGLRSALTFAWMLLIAVELT
                     AANSGLGYLLTDGRETGRADLIIVAIVVLALLGKAGDSLLQRLKGRLLGWQDVVDSRQ
                     S"
     gene            complement(3882..>4387)
                     /locus_tag="PLL14_05010"
     CDS             complement(3882..>4387)
                     /locus_tag="PLL14_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG03314.1"
                     /translation="SGPTHWQSWFENTLPATRRVEQVDWERPGLSEWSERVCAAIAEQ
                     NDPVWIIAHSFGCLAAAHAGFVSGNRIRAALLVAPADPARFGEPAALLEKRLAFPSLV
                     VASRSDPWVKTSIAEHWANVWGSRYRNIGDAGHINVDAGYGPWPEGLAFFQELRQEVQ
                     LAVHWQS"
BASE COUNT          822 a         1328 c         1483 g          754 t
ORIGIN      
        1 aaaagcaaaa ccttactctt ggtgaggctg gccgctcaac atgctatagc caccaccatg
       61 gccgatagcg gtgacttcgc gagagcgcta tccatgctcg caacgggcga ccagagccga
      121 gaggtcaacc tccagcgctc gtgccagctt gtcggcggtg accagggaag gactggcaag
      181 tccgcgctcg atctcgccaa cataggagcg gttgaggttg gccctctccg ccagccgttc
      241 ctgagaccag ccgcgctcat ggcgcaactg ccggacgatg ctgggaaagc gctcgttgag
      301 gccattcata tcccgccacc gctttccagc atttcgttcc tggagacggc ctgggtaagg
      361 aagctccctg gcgggatgct gtgcgtcagc cagacgttgc cgccgatcga cgagccgcgg
      421 ccgatggtga cgcgaccgag gatggtcgct ccggcgtaga ttacgacgtc gtcctcgacg
      481 atcggatggc gcgccgtgcc tttctccagg gcaccgtcga catcgacgct gaagcgtttg
      541 gcgccgagag tgaccgcctg gtagaggcgg acccgattgc cgatgaccgc ggtttcgccg
      601 atgaccacgc cggtaccgtg atcgatgaag aagctgttcc cgatgcgcgc accggggtgg
      661 atgtcgatgc cggtcgccga gtgggcgagc tcggaaacaa tgcgcgccag cagcggcacg
      721 cccagagcgt aaagcgtgtg cgccagacgg tgatggatga tggccagcgt tccggggtag
      781 cagaggacaa tctcgtcaac gctgcgcgct gccgggtcac cacggaaagc ggcctcgaca
      841 tcgctatcga gcaggacgcg aatgcctggc agggccgcgg caaagtgctg gacgacggcg
      901 atcgactgcg tctcgattgt cgtcggatcg acctccggct ggtggcggga agcgtgtgtc
      961 agctccaggc gcacctggtc gagcaggttg cgcaaggcgg cgtcgagggt atgcccgaca
     1021 taaaagtcct cgctgtggcc ctgctgcaga tcaggcggtc cgaggcgcat cgggaagagg
     1081 gcgccgcaca gacgttcgac gacgtcgcgc agagcggcac gcgagggaaa ctcgcgacca
     1141 ccggattcga gagggcgctg ctgcgcggta cgccattttt cgcgggcggc gcgcagatcg
     1201 cggacgatat cctccagacc ccagagatcc gaggtcgagg tatcgggtgt tgaacgaagt
     1261 ggggcgctca tcgacgtttt ttgcctcagt cctgcaccca gggcagcccg tggtagcgcc
     1321 agccgccggt cgcgccgcgc tggccgtcgg gagtgatttc gccctcgaag ccctccagaa
     1381 cattgaacac ccggctgaat ccagcccgcg tagcggcttc ggcggcggct gccgagcgct
     1441 tgccgctgcg gcaaatgagg agaatggcat cgttcttgct ggccttgcct gccagttcgc
     1501 gggtgaagcg cgggttcctg gtcaacgccg ctcccgactg ccactgcacc agcggcgtgc
     1561 cgggcacgtg gccgacgtat ttgtattctt cggcagtacg cacgtcgatc aggatcgcgg
     1621 cgcccgcggc tgccaatgcc caggcctcgc gcggcgcaac ggtaccggcg tagggcaggt
     1681 tcccgagcgc ggcgcgcgcg cttgccgcgg cgaggatgtg gtgcagatcg gtcggctgcg
     1741 cagcagcatc gggaggccga tactcggtcg tgggggaggc agcatcgggt tgggcggtgg
     1801 caagcatcga agtctcctgg aaatgaaaag ctggccgttg cggtgatgga atcggcctgt
     1861 gggcatcact ctagccgcag ggcttgcggc agcgaacgaa gaaaaactgc gttgcttata
     1921 aagggatcgc gcgaacatgc gctcagacgc aggcgccgct ggccacgctg tgcgtggtcg
     1981 cggggtgtct gccggcgatt acgatctgcg tcgtgcggtc tcggggtgcc cggtattggc
     2041 agggaccggg cgaaactttg attcggtgtg ggccatcggt gctgctcaga tggcaaagtg
     2101 caggtgggca gcctgcaccg gcggccacgt tggcggtgca ctggccaggg cataggaaag
     2161 atcgaaggaa ttggtcgtgc catcgctcaa cagggtcagg actttggctt cctttcgcgc
     2221 cagttgcggg ttacgccggt cgcgcggacg cggtgcaccg atcaccacct cgccactgat
     2281 ccgcccgggc tggctggcaa gcacgatcac gcggtcgctg aaatagacgg cttcgctgac
     2341 ctcatgcgtg accagcagca gggttgtgcg acgacggctg gcgatcgaca gcaacaggtc
     2401 ttgcaggtgc ttgcgagtga acgcatcgac cgcgctgaaa ggctcgtcga gcagcagcag
     2461 cgagggctgg gaacacaagc cccgggcgat ggctaccctt tgcgccatgt ctcccgagag
     2521 ctgccttggg taggcgcctg cgcaattgac gaggcccatt tcggacagca gcgcagcgac
     2581 gaggggatgt ccccgtccct tgatccccaa ggcaaacccg acgttctcgg caacggttaa
     2641 ccacggcaac agacgaggct cctgaaaaat caggcctaca tcacgggaac gcaagctcgg
     2701 tggctgacca ttgatcagca cttcgccggc gaagcacgaa tcgagagcgg cgataatccg
     2761 caagagggtg ctttttccac aaccccgggg tccgaccaga ctgacgactt cgccggtgtt
     2821 gatccggaaa tggacgtttc tgagaacggg caggtccgcg aagctctttg ccgtcaccgt
     2881 caggtcgagc aactgttggg acattgtggg gtattcctca ggattgccgg ctatcgacaa
     2941 catcctgcca gcccagcagg cggcctttca gccgctgcag caggctgtcg ccggctttgc
     3001 cgagcagggc caggacgacg atggcgacga tgatcagatc ggcgcggccg gtttcgcggc
     3061 cgtcggtgag cagatagccg agcccgctat tggcggcggt cagttcgacg gcgatgagca
     3121 gcatccacgc aaacgtcaga gcgctgcgca gaccgctgaa cagattgggc agggccgctg
     3181 gcaggaagac ccgggtgatc agcagggatc cgctcaggcc gaagaggcct ccgacctcaa
     3241 gcagcttgtc atcgacgtca tggatgccgg cgacgacgtt gagatagacc gggaagaagg
     3301 cgccgatggc gatgagagtg atcttcgtcg cttcacctga tcccagccaa agcagcgcta
     3361 acggcaccca ggcgagcaac gggacggtac gtagggtccg gaaggtcgga ccgaacagcg
     3421 tttcgacacg ctgcgacagg cccgccgcgg tgcctgccag aattcccagg acagctccga
     3481 tgctgaatcc ggtcagaagg cgcagcgtgc tgatgccggc atgcgtcagg agagcaccat
     3541 tttccacgag ttcagccagt gtgccgagca gttcgctggg cgatggcagg aggcgcgcgc
     3601 tgacccaccc ggatccgctc agcgtctgcc agatcagcat caggatggcc gggatccaca
     3661 gaccgtggct gtagcggagc caccttctgt acgcccgggg ccggttcctg gcaagcaagg
     3721 ccggtgccct cgaaaggcct gtttgactca tccggcagtt cctcggcgca ctacggtgtt
     3781 ggtcggggtc ggcttgcggc gcttgccaat cgccatattg acgccggatt tattcacgat
     3841 gcgtcgaaag actggggaca tcggtattcc ttgaagaggg ttcagctctg ccaatgaacg
     3901 gccaactgga cttcctggcg caactcctgg aagaaggcga gtccctccgg ccacgggccg
     3961 tagcccgcat cgacattgat gtgaccggcg tcaccgatat tccggtagcg gctaccccag
     4021 acgtttgccc agtgctccgc gatgctggtc ttgacccatg gatcgctcct gctggcgacg
     4081 accagactcg gaaaggcaag ccgtttttcc aggagcgcgg ccggttcgcc gaagcgagca
     4141 ggatctgccg gggccacgag cagggccgca cggatgcggt tgccgctgac aaagccggcg
     4201 tgggcggccg ccagacagcc aaaactgtga gcaatgatcc aaacggggtc gttttgctcg
     4261 gcaatggccg cacacacacg ttccgaccat tcggagagac cggggcgctc ccagtcgact
     4321 tgttcaactc gtcgggtagc gggcagcgtg ttctcgaacc agctctgcca atgggttgga
     4381 ccactat
//
LOCUS       DAOSIT010000153         4384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17629_length_4384_cov_11.972742, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000153 DAOSIT010000000
VERSION     DAOSIT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17629_length_4384_cov_11.972742"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLL14_05015"
     CDS             <1..548
                     /locus_tag="PLL14_05015"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007022038.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HOG03315.1"
                     /translation="VRHRYRDLFRSHLDLDPLSCNTSELAAVARRHIDIAFDDADRDT
                     WLELLMSHVIEPRLGNDALCFVVDYPPSQAALARLRVDAEGQQVAARFELYYRGVELA
                     NGYHELLDAGEQRRRFAADLREREALGLPAIPLDEKLLAALEHGLPDCSGVALGFDRL
                     VMLAVGGEALDEVMAFPASHR"
     gene            complement(605..1345)
                     /locus_tag="PLL14_05020"
     CDS             complement(605..1345)
                     /locus_tag="PLL14_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOG03316.1"
                     /translation="MSFPPNASARAPTPSADAHPLRSLLCVLAIIAVVWQLPYGRLML
                     YPLSLLATCAHELGHGFTAMLVGAEFRSFVLHADGSGLALWQGSTGRLATALVAAGGL
                     LGPTLAGSLLLILARSSRLSRTLLWLLAGGLLAVVVCWTRNPFGVVFLVGAATFFAAV
                     ALWLGERGATFVVRLTGAVLCLSWLQHVDYLFSAGATINGMRHASDTAVIAQALWLPY
                     WFWGAAIALLSFAVLACGLWAATRPHPP"
     gene            1437..2156
                     /locus_tag="PLL14_05025"
     CDS             1437..2156
                     /locus_tag="PLL14_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03317.1"
                     /translation="MKGILSLLAKAKLIELSEDEQLTASEERPAAVPDAPPAPPAEVV
                     ETRMHAAGTAAAAPDLPSLADACADLDGATFSDLFASAGVPASPYPAEKLLRLLDGLR
                     AMDAGTRKSAVLAMDAADDNWQIDDCVRDADLKIAALEEHKRRLAAQLQERERQSGEL
                     VNQIKVVLDEASAAIRQQISELEQLLEREVTRADQETTSVEAGLRAAREATAREARRM
                     DAEIERLREIPNTFKAPGAAH"
     assembly_gap    2276..2375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2376..3736
                     /locus_tag="PLL14_05030"
     CDS             <2376..3736
                     /locus_tag="PLL14_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HOG03318.1"
                     /translation="GVNVRFVIQDDIPTLATVFESGAAQCAWRTSDFWAQEQPNLRNA
                     GLDGKAVMIVDNTQGGDAVIARDASVKAVEDLAGRSVALLQFTPSHGMLIDALDNSSL
                     TARKKDSVKMVFINAEEGTAGVRAALESGAVDAAVLWDPDLALALRNVKGAHVVYSTK
                     TATNLIFDVMVCDSRLLARPEGQAVVQKFVAGWMDGVQAARSNPDNAVEALVRTQEFF
                     KLLADKEGRPFVKSLFANLVWTGVEENARILGLAGGTNHYERVYKRFDEIYRKAGALA
                     NPKSPVIAPQDSFDYRFIKSLLGENKAAADAAAKPQSTFTQSALKEATQQEAMVTKPV
                     TVGFASGSAELTKRAQKTVDTEMVPFIENNGKAYFEVSGNSDSTGTREVNLRLSAARA
                     RAVVDYLVTQWEFPRERFKIVGNGPDRPLCVEANAASEGLSAEDCRAMNRTTRVAVFG
                     GR"
     gene            3762..>4384
                     /locus_tag="PLL14_05035"
     CDS             3762..>4384
                     /locus_tag="PLL14_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG03319.1"
                     /translation="MTEFWNPYAPLDSRRRRLLGVGAALTVLLLWSVVAASGLVSSTK
                     LPAPWEVLAALSYLSWHDGGSMLLSATLWSVGRLLVAGVLVISIGIPIGIVMGAAPQV
                     NAALSPLVDPFRSAPVVALLPILVMWLGIGETMKIAFLFIGAVVYLIPMVRDAIQAVP
                     QSYWIGARDLGATPWECIHRAVIPMAMPRIADAVIVAFSVMWTYITVA"
BASE COUNT          766 a         1365 c         1452 g          701 t
ORIGIN      
        1 gcgtccgcca tcgttatcgc gacctgtttc gcagccatct cgatctcgat ccactgagct
       61 gcaacaccag cgaactcgcc gccgtggcca ggcggcacat cgacatcgcc ttcgacgatg
      121 ccgatcgtga tacctggctg gagttgttga tgagccatgt catcgaaccg cgactcggta
      181 acgatgccct gtgtttcgtg gtcgattacc cgccgtcgca agcggcactc gcgcgcctgc
      241 gcgtggacgc ggaaggccag caggtggctg cgcgttttga gctgtactat cgcggcgtcg
      301 agttggccaa tggttatcac gaattgctcg atgccggcga acagcgcaga cgttttgccg
      361 ccgatctgcg cgaacgtgaa gcgctgggcc tgccggccat accgctcgac gagaaactgc
      421 tggccgcgct cgaacacggt ctgccggact gcagtggtgt cgcgctcggc ttcgaccgcc
      481 tggtgatgct cgccgtcggc ggtgaagctt tagatgaggt gatggccttt cccgcgtcgc
      541 accggtaagg cctcagcggc aatcctcagg tcggcctcgc cgaagcttcc gtcgccggca
      601 ccgatcacgg cgggtgtggc cgagtcgccg cccacagacc acaggccagg acggcaaacg
      661 acagcagggc gatcgcggcg ccccagaacc agtacggcaa ccacaacgcc tgggcgatga
      721 ctgccgtgtc ggaggcgtgg cgcatgccat tgatggtcgc accggccgag aacagatagt
      781 cgacgtgctg cagccagctc aggcagagta ccgcgccggt caggcgcacg acgaaggtgg
      841 cgccgcgctc gcccagccag agcgccacgg cggcgaagaa ggtggcggcg ccgaccagga
      901 agaccacccc gaacggattg cgcgtccagc agacgacgac cgccagcaga ccgccggcga
      961 gcagccagag cagcgttcgc gacagccgcg acgagcgcgc caggatcagc agcagactgc
     1021 ccgcaagcgt cggacccagg aggccgccgg cagccaccag agcggttgcg aggcgacccg
     1081 tcgaaccctg ccacaaggcc agacccgagc cgtcggcatg cagcacgaag ctccggaatt
     1141 cggcgccgac gagcatggcc gtgaaaccgt gcccgagttc gtgggcacag gtcgccagca
     1201 gcgagagcgg gtagagcatc aagcggccgt aaggaagctg ccagaccacg gcgatgatcg
     1261 ccagtacgca gagcagactg cgcagcggat gcgcgtcggc agagggtgtc ggcgcgcgtg
     1321 ccgacgcgtt tggcgggaag ctcattgtgg ggcaaattct tgcattgcga cctttcgttc
     1381 gtataatccc tgactctgcc gggttctgca accaggcaag cattccggga gtagggatga
     1441 agggtatctt gagtctgctg gcgaaagcaa agctgattga gctttcggaa gacgaacaac
     1501 tgactgcctc ggaggaacgc ccggcggcgg tgccggacgc cccccctgcg ccacctgccg
     1561 aggtagtcga gacccgcatg cacgcagcgg ggactgccgc cgccgcgccc gacctgccgt
     1621 cgctggccga cgcctgtgcc gacctcgacg gcgcgacctt cagcgatctc ttcgcgtcgg
     1681 ccggtgtccc cgcatcgccg tatccggccg agaagctgct gcgcctgctg gatggactgc
     1741 gggcgatgga cgccggcacg cgcaagtccg ccgtactggc gatggatgcc gccgacgaca
     1801 actggcagat tgacgattgc gtacgcgacg ccgatctcaa gattgcggcg ctcgaggagc
     1861 acaagcgtcg cttggcggcg cagctgcagg agcgcgagcg gcagtcgggc gaactcgtga
     1921 accagatcaa agtggtactt gacgaagcga gcgcggcgat tcgccagcag atttccgaac
     1981 tcgagcagtt gctcgaacgc gaggtcacgc gtgcggatca ggaaacgacc agcgtcgaag
     2041 ccggtttgcg cgccgcacgc gaggctaccg cgcgcgaggc ccgccgcatg gatgccgaga
     2101 tcgagcgcct gcgcgaaatt cccaacactt tcaaggcgcc tggcgccgca cactgaagtc
     2161 ccgagcaagg agaagatcac atggctcaac tcacgccgct ggccaagggg ctgattaccg
     2221 tcatcgttct gggcgtggcc ggttcgctgg cctggaattt cgggctcaag gagcgnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagggc gtcaatgtgc gcttcgtcat
     2401 ccaggacgac attccgacgc tggccaccgt cttcgagtcc ggtgccgccc agtgcgcctg
     2461 gcggacctcg gatttctggg cacaggagca gcccaacctg cgcaacgccg gtctcgacgg
     2521 caaggcggtg atgatcgtcg acaacaccca gggtggcgac gcggtgattg cccgcgatgc
     2581 gtcggtaaag gccgtcgagg atctggccgg tcgctcggtc gcgctgctcc agttcacccc
     2641 ctcgcatggg atgctgatcg acgccctcga caattcctcg ctgaccgcgc gcaagaagga
     2701 cagcgtcaag atggtgttca tcaacgccga ggaggggaca gccggcgtgc gcgccgcgct
     2761 cgaatccggt gccgtcgatg cggcggtgct gtgggacccg gatctcgcgc tggcgctgcg
     2821 caacgtcaag ggggcgcatg tcgtctattc gaccaagacc gcgaccaacc tgatctttga
     2881 cgtgatggtc tgcgactcgc gcctgctggc caggccggaa gggcaggcgg tggtgcagaa
     2941 gtttgtcgcc ggctggatgg acggcgtgca ggcggcacgc agcaacccgg acaacgccgt
     3001 ggaagcgctg gtcagaacgc aggaattctt caagttgctg gccgacaagg aaggccggcc
     3061 attcgtcaag agccttttcg ccaacctggt atggaccggt gtcgaggaaa acgcgcgcat
     3121 tctcggtctg gccggcggta ccaatcacta cgaacgggtt tacaagcgct tcgacgagat
     3181 ctatcgcaag gccggtgcgt tggcgaatcc gaagtcgccg gtgatcgcgc cgcaggacag
     3241 cttcgactat cgcttcatca agtccctgct cggggaaaac aaggcggccg cggacgccgc
     3301 cgccaagccg cagagcacct tcacgcagtc ggcgctgaag gaagcgacac agcaggaagc
     3361 gatggtgacc aagccggtca cggtgggttt tgcctcgggt agcgccgaat tgaccaagcg
     3421 cgcgcagaag acggtcgata cggagatggt gccgttcatc gaaaacaacg gcaaggccta
     3481 cttcgaggtc agcggcaatt ccgattcgac cgggacccgc gaggtgaatc ttcgcctgtc
     3541 ggcggcgcgg gcgcgggcgg tggtcgatta tctggtgacg cagtgggagt ttcctcgcga
     3601 acgattcaag atcgtcggca atggcccgga tcgaccgctt tgtgtcgagg ccaacgcagc
     3661 cagcgagggg ctgtcggccg aagactgccg ggcgatgaac cggaccaccc gggtggccgt
     3721 tttcggcggc cgctgaaccc gtgccgccag tctgcccatc gatgaccgaa ttctggaacc
     3781 cctacgcgcc gctcgacagc cggcggcggc gcctgctcgg tgtcggcgcg gccctgacgg
     3841 tgctgctgct gtggagcgtc gtcgccgcat cgggactggt cagctcgacc aagctgccgg
     3901 cgccctggga agtgttggcg gcgctgtcgt acctgagctg gcatgacggc ggcagcatgt
     3961 tgctctccgc caccctgtgg tcggtcggtc gcctgttggt cgccggcgtg ctggtgatca
     4021 gcatcggcat tccgatcggc atcgtcatgg gcgcggcacc gcaggtgaac gccgccctgt
     4081 cgccgttggt cgatcctttc cgttccgcgc cggtggtggc gctgctgccg atcctggtga
     4141 tgtggctggg catcggcgag acgatgaaga tcgccttcct gttcatcggc gccgtggtgt
     4201 acctgattcc gatggtgcgc gacgccatcc aggcggtgcc gcagagctac tggatcggcg
     4261 cccgcgacct cggggccacg ccgtgggaat gtatccaccg agcggtcatc ccgatggcca
     4321 tgccgcgcat cgccgatgcc gtcatcgtcg ctttctcggt gatgtggacc tacatcaccg
     4381 tcgc
//
LOCUS       DAOSIT010000154         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_17922_length_4329_cov_9.726720, whole genome shotgun sequence.
ACCESSION   DAOSIT010000154 DAOSIT010000000
VERSION     DAOSIT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17922_length_4329_cov_9.726720"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="queG"
                     /locus_tag="PLL14_05040"
     CDS             <1..986
                     /gene="queG"
                     /locus_tag="PLL14_05040"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG03320.1"
                     /translation="RLMRWLELGRHGEMDYMAKHATLRATPQGLLPDTQSVISARLPY
                     WPAAADAWQLLAASERAYISRYALGRDYHKTMRRRLQQLIDRLHREAAALHLPKPFAC
                     RAFSDSAPVLETEFAVQSGVAWRGKHTLTLTRSGSWHFLGEIYTTLPLPPDRPIGDHC
                     GDCRRCLDCCPTQAIVAPYEVDARLCISYLTIELQGAIPEALRPLIGNRIYGCDDCQL
                     CCPWNRFADIGDPDFGVRNGLDAAPLATLFAWSEDTFNTRLAGSAIRRIGHQRWLRNI
                     AVALGNAESSPTNLAALASRQDDPSPLLREHVGWALARHAGKCARPGEGGR"
     gene            complement(1046..2686)
                     /locus_tag="PLL14_05045"
     CDS             complement(1046..2686)
                     /locus_tag="PLL14_05045"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOG03321.1"
                     /translation="MEREAMAYDVVIVGAGPAGLSAAIRLKQLDPELAVMVLEKGSEV
                     GAHILSGALFESRALDELLPDWQALGAPLNTPVTEDQVYLYRSETAAVKLPGFAVPAP
                     MHNAGNYLISLGSLCRWLAAQAEGVGVEIFPGFAAAEILYHDDGSVKGVATGDMGRDK
                     EGKPKDSFQPGLELHAKYTLLAEGSRGQLGKELIARYDLAAGATAQHYGLGIKELWQI
                     DPAKHRPGLVVHGAGWPLSEHGATGGSFLYHMEDNQVAVGLIADLNYANPYLSPFEEF
                     QRFKLQVEIRKTLEGGKRLCYGARAITKGGLNSLPKQTFPGGLLIGCDAGTLNFAKIK
                     GIHTAMKSGMLAAETVHAALKAGDEGRSDLVAYASAFESSWLHAELYQARNFGPALHK
                     FGTFLGGAFNWIQQNLFGGHMFFTLKDRSNDHAQLVPAAHCQPIDYAKPDGQLTFDRL
                     SSVFVSNTAHEEEQPVHLQLLDCSKAIAVNFKEYASPEQRYCPAGVYEIVEDASGPRL
                     QINASNCLHCKTCDIKDPTQNIRWVAPEGGGGPNYPNM"
     assembly_gap    2917..3016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3017..4327)
                     /locus_tag="PLL14_05050"
     CDS             complement(<3017..4327)
                     /locus_tag="PLL14_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG03322.1"
                     /translation="MPRGLMQDRPLLISSLIEHAACFHPQAEIVSRTTEGPIHRCTYA
                     DIHRRAKQLANALDVRGVGPGDRIATLAWNGYRHMELYYGVSGMGAVLHTINPRLFPE
                     QIEYIVNHAEDQFLFFDLSFVPLVERLAKALPTVRGFVVMTDREHLPECSIPRLLCYE
                     ELLGAQTSDYQWPQFDETTASSLCYTSGTTGNPKGVLYSHRSSVLHSWAACATDGLAI
                     GAGETALLVVPMFHVNAWGMPYAGAMCGARLVMPGPALDGRSVYQLLRDEKVTLALGV
                     PTVWLMLLQYVDAERLTPKVDLCLRRVVIGGSSAPRAMSERFETTFGAFVVHAWGMTE
                     MSPLGTVCNLLPKHGGYTLEQRLAVQEKQGRAVYGVEMKIVDDDGASLPHDGMASGHL
                     LVRGPWITCGYYRDEGSSPLDEAGFFDTGDVATIDADGYMQITDR"
BASE COUNT          797 a         1394 c         1261 g          777 t
ORIGIN      
        1 caaggctgat gcgctggctg gaactggggc ggcacggcga aatggattat atggccaaac
       61 acgcaaccct gcgcgcgacg ccacaaggcc tgctgccgga cacccagtcg gtcatttccg
      121 cccgcctgcc ttactggcct gcggcggccg acgcctggca gcttctcgct gccagtgagc
      181 gagcctacat ctcccgctat gccctcgggc gcgactatca caagacgatg cgtcggcgac
      241 tccagcaact catcgaccgc ctgcatcggg aagcggcagc gctacacttg cccaaaccct
      301 ttgcctgccg tgcgttttcc gattcggcac cggtgctgga aaccgagttt gcggtgcagt
      361 cgggcgttgc ctggcgcggc aagcacacgc tgaccctgac gcgttcagga tcctggcatt
      421 ttcttggcga aatctatacc accttgccgc taccccccga cagaccgatc ggcgatcact
      481 gcggtgactg ccgtcgttgc cttgactgct gtcccaccca ggccatcgtg gcgccctacg
      541 aggtcgacgc ccgcttgtgc atttcctacc tgaccatcga actgcagggc gccatccctg
      601 aagcgctgcg ccccttgatt ggcaatcgca tctacggttg cgacgattgc cagttgtgtt
      661 gcccctggaa ccgctttgcc gacatcggcg atcctgactt cggcgtgcgc aacggactcg
      721 atgcggcgcc tctggcaacc ctcttcgcct ggagcgagga cactttcaat acgcgcctgg
      781 ctggcagtgc gatccgccgc atcggccacc agcgctggct gcgcaacatc gccgtcgcac
      841 tcggcaacgc cgaaagctcg ccgacaaacc tcgcggcgct ggcttcgcgg caggacgacc
      901 cctcgcctct gctgcgcgag cacgtcggct gggcgctggc ccggcatgca gggaaatgcg
      961 cacgccctgg cgaaggcggg cgctgagcga ccggccgtcc gcaggagcca gggcccgcgc
     1021 ctacgagccg ccgggaatga cgcgctcaca tgttcgggta gtttggccca ccgccgcctt
     1081 cgggtgccac ccagcggatg ttctgcgtcg gatccttgat gtcgcacgtc ttgcagtgca
     1141 gacaattcga ggcattgatc tgcagacgcg gcccgctcgc gtcctcgacg atctcataga
     1201 cgcccgccgg gcagtaacgc tgctcgggcg aggcatattc cttgaaattg accgcgatgg
     1261 ccttgctgca gtcgagcaac tgcagatgca cgggttgctc ctcttcgtgc gcggtattcg
     1321 agacaaatac cgacgacagc cgatcgaaag tcagttgccc gtcgggcttg gcgtagtcaa
     1381 tgggctgaca gtgtgccgcc gggacaagct gcgcgtggtc gttgctgcgg tccttcagcg
     1441 tgaagaacat gtggccgccg aacagattct gctggatcca gttgaaggcc ccgcccagga
     1501 aggtgccaaa cttgtgcagc gccggcccga aattgcgtgc ctgatagagt tccgcatgca
     1561 gccaggaaga ctcgaaggcg ctggcatagg ccaccaggtc gctgcgcccc tcgtctccgg
     1621 cctttagcgc cgcatggacc gtctcggccg ccagcatgcc ggacttcatg gccgtatgga
     1681 tacctttgat cttggcgaaa ttgagcgtcc cggcatcaca gccaatcagc agtccgccgg
     1741 ggaaagtctg tttcggcaga ctgttcagtc cgcccttggt aatcgcgcgc gcgccataac
     1801 acaggcgctt gccgccttcg agagtcttcc ggatttcgac ctgcaacttg aagcgctgaa
     1861 attcctcgaa aggcgaaaga tacggattgg cataattgag gtcggcgatc aggccaaccg
     1921 cgacctggtt atcctccatg tgatagagga aggaaccgcc ggtggcaccg tgctcgctga
     1981 gcggccagcc ggcaccatgc accaccagcc ccggtcgatg ctttgcggga tcgatctgcc
     2041 agagttcctt gatcccgagg ccatagtgct gcgccgtcgc gccggcagca agatcgtagc
     2101 gcgcgatcag ttccttgccg agctggccac gcgagccttc ggcaagcagc gtgtacttgg
     2161 cgtgcaattc caggccgggc tggaaactgt ccttcggctt gccttccttg tcgcgcccca
     2221 tatcgccggt ggccacgccc ttcaccgaac cgtcgtcgtg atagaggatt tcggcggcgg
     2281 cgaaaccggg gaagatctcc acgccaacgc cttccgcctg tgcggccagc caacgacaga
     2341 ggctgcccag gctgatcagg taattgcccg cgttatgcat cggtgccgga acggcaaatc
     2401 ccggcagctt gaccgccgca gtctcgctgc gatagagata gacctgatct tcagtgaccg
     2461 gggtgttcag cggtgctccc agcgcctgcc agtctggcag cagctcatcg agtgcccgac
     2521 tctcgaagag cgccccagaa agaatgtgcg ccccgacctc cgaacccttt tccagaacca
     2581 tgaccgccag ttcaggatcg agctgtttga ggcgtatcgc cgcgctcaaa cctgccggac
     2641 ccgcaccgac aatcactacg tcatacgcca tcgcttcccg ttccatcacc gctcctcaag
     2701 gtatctcaat cgacccggtt ccgaccggag gcccaccgac agcaggcagg gctgcttggg
     2761 cgacctcgat cagcctgcat acgtcccgcg atatacccga gtctgcgtgc ccggtcaacg
     2821 actgtgtggc aggcgataat ccctgaacat ttcgcggagc ctgagcttgt gcagtttgcc
     2881 ggtggcggta tgcggcagtt cgtcgacgaa agcgacnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnngcgg tcggtgatct gcatgtaacc gtcggcgtcg atggtcgcca
     3061 cgtcaccggt gtcgaaaaac cccgcttcgt cgagagggga agaaccctca tcacggtagt
     3121 agccgcaggt aatccagggt ccccgcacca gcaggtgacc agaggccatg ccatcatggg
     3181 gcagactggc gccgtcgtca tcgacaatct tcatctcgac gccatagacc gcccggccct
     3241 gcttctcctg gacggccaga cgctgctcga gcgtataacc gccgtgcttc ggcaacagat
     3301 tgcagaccgt ccccagcggc gacatttcgg tcatccccca ggcgtgcacg acgaacgcac
     3361 cgaaagtcgt ctcgaagcgt tcgctcatcg ccctcggcgc cgacgaaccg ccgatcacga
     3421 cgcgccgcag acagaggtct actttcggcg tcagccgttc tgcatcgaca tattgcagca
     3481 gcatcaacca gaccgtgggc acaccgaggg ccagggtcac cttctcatcg cgcagcaatt
     3541 ggtagacact cctgccgtca agagccggac cgggcatgac cagcctggct ccgcacatcg
     3601 cgcccgcata gggcatgccc caggcgttga cgtgaaacat cggaaccacc agcagagcgg
     3661 tctcgccagc gccgatggcc agaccgtcgg ttgcacaggc ggcccaggag tggagcaccg
     3721 aggagcggtg ggagtagagc acgcctttgg gattgccagt ggtaccggac gtgtagcata
     3781 gcgacgacgc cgtcgtctcg tcgaactgcg gccattgata gtcactcgtc tgcgcaccga
     3841 gcagttcttc atagcacaac aagcggggaa tgctgcactc cggcaggtgt tcgcggtcgg
     3901 tcatcacgac gaaaccacgg accgtcggca acgcctttgc cagcctttcg accagcggca
     3961 cgaagctcag gtcgaaaaac agaaactgat cttcggcatg gttgacgatg tactcgatct
     4021 gctcggggaa caggcgcggg ttgatcgtgt gcagaaccgc tcccatccct gaaacgccgt
     4081 aatacagttc catgtgccga taaccattcc aggccagcgt cgcgatacga tcgccgggcc
     4141 cgaccccccg aacgtccagg gcattggcca gttgcttggc gcggcggtgg atatcggcat
     4201 aagtacagcg atggatcggg ccctcggtcg tgcgtgaaac aatctccgcc tgcggatgaa
     4261 aacaggccgc gtgttcgatc agcgacgaga tcaacagcgg tcggtcctgc atgagtcctc
     4321 ttggcatcg
//
LOCUS       DAOSIT010000155         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18002_length_4314_cov_8.496126, whole genome shotgun sequence.
ACCESSION   DAOSIT010000155 DAOSIT010000000
VERSION     DAOSIT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18002_length_4314_cov_8.496126"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLL14_05055"
     CDS             complement(<1..893)
                     /locus_tag="PLL14_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOG03323.1"
                     /translation="MPDDRSRLAFVDALKALAFQLIVLHHLAFYGPMSDHAYPLAPVL
                     MTWLSQDARMAVQAFLVIAGFLAARSIAPNGVLLSNEPLVLLQQRYLKLVTPFLAAVV
                     IGIVCAAIARELMAHDSIPGPPTLPQVLAHALLLQGILGYDGLSAGVWYVAIDFQLFT
                     LLVAILWLARGVAQGGAETAAVGASLVASLALASLYHFNRDAGWDNWGVYFFGSYGLG
                     VVCCWATNGRRPLVWLLPLVTAVIAALLLDYRPRIAVALLVALALSLARMLGFLEIWP
                     RSRMLAYLGRISYSVFLIHFP"
     gene            complement(901..>2535)
                     /locus_tag="PLL14_05060"
     CDS             complement(901..>2535)
                     /locus_tag="PLL14_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03324.1"
                     /translation="DSSAEGITITAPDGSIIAVNRAFTEITGYREDEVIGRNPRMLQS
                     GRQKRSIYREMWQTLGRYGRWRGELWNRRKDGQPFLESLTISAVKDQDGHLTHYVGVF
                     SDITELRKAHDQLDHQAHHDPLTGLPNRLLLGDRLHKALQRAHRDESGLAVLFIDLDR
                     FKNINDTLGHQVGDRVLCEVAWRLSRLMRESDTVGRLGGDEFLIVIEGVVGPTAVSHI
                     AEKILNILQDSPVTVEQEFFVGASIGISVFPQDGDDAETLMKHADVAMYRAKERGRNA
                     YEFFTKDLTQSSLARLQMETDLRRAIERDELQVYLQPQFSLTTGRVVGAEALVRWFRF
                     ERGLVMPGEFIKLAEESGLIVPIGEWVQHAACCQWADWVAAGFDPGVLSVNVSGVEFA
                     RGQIQETARKALEESRLPAQFLELEITESAIMSQAENSVQVLDDLRAMGLGLVIDDFG
                     TGYSSLAYLKRLPLNKLKVDQSFVRGLPGDTEDCAIVRAVIALAHSLQLTVIAEGVEV
                     ETQREFLKREGCDEMQGYLRGRPMPFAEYRQRFLDG"
     assembly_gap    2536..2635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2636..>4314)
                     /locus_tag="PLL14_05065"
     CDS             complement(<2636..>4314)
                     /locus_tag="PLL14_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03325.1"
                     /translation="QQLIFENADTGIVIVRDRIIQRCNRRFAEILGYAPEELLGQSTR
                     IYYPSEKAWLETGRQAYAAIVETGIYRGDTLFHRRDGTPFCCYITGSMINPPDPTQGY
                     VWLYEDVSEKRAATTAMDALLREQTLIFERAPVGIAFVRDRVIQRCNPSFERIFGYAP
                     GQLIGQSTRICFASHEAWWAKGEYARMMIDEVGKFVGEVEYRRGDGNPLWCQVTGSLL
                     NPAKPEEGHVWLFEDVTEKRQASRAMDALLREQTLIFERAPIGIAFLRDRIIKRCNPG
                     FERIFGYLPGQMLGQSTRVCFASEAEWLAAGERAYAGIDQSGRFIGEIEYRKADGTPI
                     WCQVTGSLLNPDNRDEGHVWLYEDVSARRAAEEALVESLWEQQLIFDNAMIGISYQRE
                     RTILRCNRRCEEIFGYPPGGLRGQSTRVLFGSQEEWEEVGREVYRNGPTTETFDGEFL
                     YRRRDGTSIWVHVIGRTIGDDPEERTWIWTYEDVTAHRAAEQALQQTQLELEGRVAER
                     TLALSQQLHFMRQLVEAIPGPVFYKNREGRYLGCNQAFLALLGKPRSAVIGA"
BASE COUNT          885 a         1364 c         1197 g          768 t
ORIGIN      
        1 atcgggaagt gaatcagaaa taccgagtag gaaatccggc caaggtaggc cagcatgcgt
       61 gaccgcggcc agatctccag aaaacccagc atgcgtgcca gactgagcgc cagggccacc
      121 agcaacgcca cggcaatacg cggccggtag tccagcagca gcgcggcgat caccgccgtg
      181 accagcggca gtagccagac cagcggtcgt cgcccgtttg ttgcccagca gcagacaact
      241 cccagaccgt aggacccgaa gaagtacacc ccccagttat cccaaccggc atcgcgattg
      301 aagtggtaca gcgacgccag cgccagactg gccaccagcg aggccccaac ggcagcagtt
      361 tcagcacctc cttgtgcaac accacgggcc agccagagga tcgccaccag cagagtaaac
      421 aattggaaat cgatggccac gtaccagacg cccgccgaca gcccatcgta acccagaatc
      481 ccctgcaaca gcagcgcgtg tgccagaacc tgcggcaagg tcggcggacc gggtattgag
      541 tcatgcgcca tcaattcgcg cgcaatggcc gcgcagacga tgccgatcac cacggcggca
      601 aggaagggtg tgaccagttt cagatacctt tgctgcaaca gcacgagcgg ctcgttcgac
      661 agcaacaccc cattgggagc aatgctacgc gcagccagaa agcctgcgat caccagaaaa
      721 gcctgcactg ccatgcgcgc atcctggctc agccatgtca tcaggacggg cgccagcggg
      781 taggcatggt cggacatcgg cccatagaaa gccaggtgat gcagaacgat caactggaag
      841 gccagcgcct tcaacgcatc gacgaaggcc aacctcgacc ggtcatcagg catgcgggct
      901 tcaaccatcg aggaagcgct gtcggtactc ggcgaagggc atcggccgtc cccgcaggta
      961 gccctgcatt tcgtcgcaac cctcgcgttt gaggaactca cgctgagtct cgacctcaac
     1021 gccctcggca atgaccgtca gttgcaggct gtgcgccaag gcaattaccg cacgcacgat
     1081 ggcacagtct tccgtgtcgc cgggcaaccc gcgcacaaaa ctctgatcca ctttgagctt
     1141 gttgagcggc aaccgcttca ggtaggccag cgaagaatag ccggtgccga aatcgtcgat
     1201 caccaaaccc agccccattg cccgcagatc gtcaagaacc tggacactgt tttcggcctg
     1261 actcatgatg gcactctcgg taatctccaa ctccaggaac tgcgccggca gtcgcgactc
     1321 ctccagcgcc ttgcgcgcgg tttcctgaat ttgtccgcgc gcaaattcaa caccggatac
     1381 gttgaccgac agcacacccg gatcgaaacc ggcggccacc caatccgccc actggcagca
     1441 ggcagcatgc tggacccact cgccgatcgg cacgatcagg cctgattcct cggcaagttt
     1501 gatgaactcg ccaggcatca ccagtccccg ttcgaaacga aaccaacgga ccagagcctc
     1561 cgcgccgacg actcgacccg tggtcagcga aaactgtggc tgcagataca cctgcaactc
     1621 gtcgcgttcg atggcccgcc gcaggtcggt ttccatttgc agacgcgcca gcgaggactg
     1681 agtcaggtcc ttggtgaaga attcgtaggc attccggccg cgctccttgg ctcgatacat
     1741 ggccacatcg gcgtgcttca tcagggtttc cgcgtcgtcc ccatcctgcg gaaagacgct
     1801 gatgccaata ctggcaccga cgaagaactc ttgctcgacc gtcaccggag agtcctgcag
     1861 gatattcaga atcttctcgg cgatgtgcga caccgcggtt ggaccgacaa ccccctcaat
     1921 gacaatcagg aattcgtcgc cacccaggcg accgacggta tcggattcgc gcatcagccg
     1981 gctgagacgc caggccactt cgcagagaac ccgatcaccg acctgatgcc cgagggtatc
     2041 gttgatgttc ttgaatcggt ccaggtcgat gaacagtacg gcgagtccgc tctcgtcgcg
     2101 gtgcgcacgc tgcagtgcct tgtgcagacg gtcgccaagc agcagccggt tgggcagacc
     2161 ggtcagcgga tcgtgatgtg cctgatgatc cagctgatcg tgggctttgc gcaactccgt
     2221 gatgtcggag aacaccccga catagtgcgt gagatgccca tcctggtcct tgacggcgct
     2281 gatcgtcaag gactccagaa agggctgacc gtcctttcga cggttccaga gttcgccccg
     2341 ccagcgccca tagcggccaa gcgtctgcca catttcgcgg tagatgcttc gtttctgacg
     2401 accggactgc aacattcgcg ggttgcgccc gatgacttca tcctcgcggt agccggtaat
     2461 ctccgtaaac gcgcggttga ccgcaatgat gctgccgtct ggcgcggtaa tggtgatgcc
     2521 ctcggcactg ctgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcacc
     2641 gatcaccgcg ctgcgtggct tgcccaggag cgcaagaaag gcctgattgc atcccaggta
     2701 acgcccctcc cgattcttgt agaagaccgg gccaggaatg gcctccacga gttgacgcat
     2761 gaaatgaagc tgctgcgaca acgccagcgt acgttcggca acacggccct cgagttccaa
     2821 ctgcgtttgc tgcaaggcct gctcggcagc ccggtgggca gtgacgtcct cataggtcca
     2881 gatccaggtc cgctcttccg gatcatcgcc aatggtgcgg ccgatcacat gcacccagat
     2941 cgaagtgccg tcgcgccgac gatagaggaa ttccccgtcg aaagtctcgg tcgtcgggcc
     3001 atttcgatag acttcgcgtc caacctcctc ccactcctcc tgactgccga agagtacacg
     3061 agtggactgg ccccgcaacc cccccggtgg atagccaaat atttcttcgc aacgtcgatt
     3121 gcagcgcagg atcgtccgtt cgcgctgata ggaaatcccg atcatcgcgt tgtcgaagat
     3181 caattgctgc tcccagaggc tctcgacgag cgcctcctca gcagctcgac gcgcgctgac
     3241 gtcttcgtac agccagacat gtccttcgtc gcggttgtcc gggttgagca ggctaccggt
     3301 gacctggcac cagatcggtg taccgtccgc tttgcggtac tcaatctcgc cgatgaacct
     3361 tcccgactgg tcaatcccgg cgtacgcccg ttctcccgcc gcaagccact cggcttcgct
     3421 ggcgaaacag acgcgcgtgg attgccccag catttgcccg ggcaagtatc cgaagatgcg
     3481 ctcgaaaccg gggttgcagc gcttgatgat gcggtcacga aggaaagcga tgccgatcgg
     3541 ggcgcgctcg aagatcagtg tttgttcgcg cagcagcgca tccatcgcac ggctcgcctg
     3601 acgcttttcg gtcacatcct cgaatagcca gacgtgccct tcctcaggct tcgccgggtt
     3661 gagcaagctg ccggtaacct gacaccacag ggggttgcca tcgcccctgc gatactcaac
     3721 ctcaccaacg aatttcccaa cttcgtcgat catcatacgg gcgtactcgc cctttgccca
     3781 ccaggcttcg tgactggcaa agcatatacg ggtcgactgg ccgatcagtt ggccgggcgc
     3841 gtaaccaaaa attctttcga aactcggatt gcagcgttgg atgacgcgat cacgcacgaa
     3901 cgcaatgccg accggagcac gttcgaagat cagcgtctgt tcgcgcagca gcgcgtccat
     3961 cgccgtggtc gccgcacgtt tctcggacac atcttcgtac agccagacgt agccctgggt
     4021 cgggtctggc ggattgatca tgctgccggt gatgtaacaa cagaaaggcg taccgtcgcg
     4081 ccggtggaac agcgtgtcgc cgcggtagat gccggtttcg acgatcgccg cgtaagcctg
     4141 gcggccggtt tccagccagg ccttctcgct cggatagtag atgcgggtcg attggcccag
     4201 caactcttca ggcgcgtaac ccaggatctc tgcaaagcgg cggttacaac gctggatgat
     4261 gcgatcgcgc acgatcacga taccggtgtc ggcgttctcg aagatgagtt gctg
//
LOCUS       DAOSIT010000156         4263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18294_length_4263_cov_7.800618, whole genome shotgun sequence.
ACCESSION   DAOSIT010000156 DAOSIT010000000
VERSION     DAOSIT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18294_length_4263_cov_7.800618"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..722
                     /locus_tag="PLL14_05070"
     CDS             18..722
                     /locus_tag="PLL14_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG03326.1"
                     /translation="MAESGKLEKMLILAFSDSRKAESGGLAEADDSFEALINPESYTQ
                     EYKLKFSESGQGQGTSGKQLKYEYTQPEEMTFEFLFDNTGIIDGKPRDSVADDLKLFK
                     QVLIEYKGDSHEPRHFKLVWGQNSIFKGRVTEVSITYKMFKSDGTPIRAVAKAKFKSS
                     IEEQKRAARENKSSPDLTHVRRVKRGDTLPQLCYRIYGDPGYYIQVAEFNALENFRAL
                     RPGSDLIFPPLEKATR"
     gene            724..2490
                     /gene="vgrG"
                     /locus_tag="PLL14_05075"
     CDS             724..2490
                     /gene="vgrG"
                     /locus_tag="PLL14_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HOG03327.1"
                     /translation="MSNESTIPTPATPDVCTLTVLVEGQEISSEFHVLSLSISRELNR
                     IPAATLQIQDGDAAKGTFAVSDTDSFVPGKRIEVQLGYRSNNETVFKGIVVKQAIRIR
                     KGGSVLTVECRHDAVRMTSGARSRYFVDQKDSDVMEEVIGFYGLQKSVESTLPDLKEV
                     VQYDTSDWDFLLCRAEANGQVVLVEDDKVSVGKPLTAATAVLRIAYGATVLELDAEID
                     ARWQSKGITASSWNASDQSVVSADAKEPKAMRSGNLAAADLATVAGSDPQELRHGGKL
                     GEPELQAWADARLLRERLAKVRGRATFQGFSGVAPGRIVEITGIGARFAGEMYVSGVR
                     HRLSGGNWETDVQFGLSTECFAETYNLRPLPAAGLLPAVCGLQIGVVTTLENDPAGED
                     RIKVRMPLISSSEDGIWARLVTLDAGKQRGTYFRPEIGDEVVVGFLNDDPRHPVVLGM
                     CHSSAKPAPEAAKDDNHLKGYVSREKLRLNFDDQKKIIGLETPGGNKLTLSEDAKGIS
                     LEDQNGNRITLDDSGITIESVKDLSVKASKDLKLQGTNTELKATAAFKASGSSSAEIS
                     SPSTSIKGSATTVIQGGMVQIN"
     gene            2588..2857
                     /locus_tag="PLL14_05080"
     CDS             2588..2857
                     /locus_tag="PLL14_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HOG03328.1"
                     /translation="MPAAARITDMIVSTATLGVPVPIIPPGAPTVLIGGLPAARLGDS
                     CGADVIIKGSATVLIGGMPAARVGDPTAGGGAVMPPGVLQVNIGG"
     gene            2864..3307
                     /locus_tag="PLL14_05085"
     CDS             2864..3307
                     /locus_tag="PLL14_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOG03329.1"
                     /translation="MKQDFLGRGWSFPPEFVADRAAVEMLEEEADIASSLEILLSTAQ
                     GERLLQPLYGCNLDELLFESLDTRMKTLMADKVESAILYNEPRISLERVKLDDSQELQ
                     GLVLIEVVYRVKSTNSRFNFVYPYYKLEGTDINLSTTVNLLPDTA"
     gene            3311..>4263
                     /locus_tag="PLL14_05090"
     CDS             3311..>4263
                     /locus_tag="PLL14_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03330.1"
                     /translation="MATSTDCQQNQDPLKLIREGTSQNQRLPVALDPGYAPVDGRTTA
                     HGMVFADSYAALLKYFDATNSEAGDWKAFFGNDVSVRLAVVAVEDADAYKRNIKSYLD
                     YLDDRQNRNEGEQLRNHLGFLYGSLGTLARQLDLLKEALPADIALKSTLQNLIKSQLA
                     PAFRRLMAYYQAGNSLALVNSVAQSPETTILRAPVTTFAAVLTAGLSTDWSAGRPWET
                     YVATVLADPVVYGDAAADVFVRINHCATHALFKSIFEQFLKVFARTVSEAALALEGTL
                     NNWDRHQPHYALFLAFLRLLDYLRADINTLTRRHLDFYYRE"
BASE COUNT          943 a         1216 c         1322 g          782 t
ORIGIN      
        1 aacaagggcg aaagataatg gcggaatccg gcaaacttga aaagatgctc attctggcgt
       61 tttccgattc ccggaaagcg gagagcggcg gtctggccga agcggacgat tctttcgaag
      121 cgctgatcaa tcccgagagt tacacgcagg agtacaagct caaattctcc gaatccgggc
      181 aaggccaggg taccagcggc aagcagttga agtacgagta cacccagccc gaggagatga
      241 ctttcgagtt tctgttcgac aacaccggaa tcattgacgg aaaaccacgc gactcggttg
      301 ccgacgatct gaagctgttc aagcaggtgt tgatagaata caagggagat tcgcatgaac
      361 cacgccactt caaactggtg tggggacaga actccatttt caaggggcgt gtcaccgaag
      421 tcagcatcac ctacaagatg ttcaagagtg acggaacgcc aatacgcgcg gtagccaaag
      481 cgaagttcaa aagtagtatc gaggagcaaa agcgtgcggc cagggagaac aagagttccc
      541 cggatctgac acacgttcgc cgtgtcaaga ggggggatac gctgccgcag ctgtgttatc
      601 ggatctacgg tgaccccgga tattacatcc aggtggctga attcaacgca ctcgagaatt
      661 ttcgcgcact caggccgggc agtgacctga tctttccacc gctggaaaag gcgacgcgtt
      721 gaggtgagca acgaatccac cattcccacg ccggcgacgc ctgatgtctg cacgttaacg
      781 gtcctggttg aggggcaaga gatcagcagt gagtttcacg tcctgtcgct ttcgatcagc
      841 cgcgaattga accggattcc ggccgcaacg ctgcagattc aggatggcga tgcggccaag
      901 gggacattcg cggtcagcga tacggacagc ttcgtgccgg ggaaaagaat tgaagtccag
      961 ttggggtatc gctcgaacaa cgaaacggta ttcaagggaa tcgtcgtcaa acaggcgatc
     1021 aggatacgca agggtggcag tgtgttgact gtcgaatgcc ggcatgatgc ggtgaggatg
     1081 accagcgggg ccaggagccg ttattttgtc gaccagaagg acagcgatgt catggaggaa
     1141 gtgatcggct tttacgggtt gcagaaatcc gtcgagtcga ccctccccga cctgaaggaa
     1201 gtggttcagt atgacaccag cgactgggat ttcctgcttt gtcgggccga ggcgaatggc
     1261 caggtcgtgc tggtggaaga tgacaaggtc agcgtcggca aaccgctgac ggcggccacg
     1321 gccgtactca ggattgctta tggcgcgacg gtactcgaac tggacgcgga aatcgacgcg
     1381 cgctggcaaa gcaaagggat taccgcgagt agctggaatg ccagcgacca gtccgtagtc
     1441 agcgccgacg cgaaagagcc gaaggcgatg cgcagcggca atctcgcggc cgccgacctg
     1501 gccaccgtgg ccggcagcga cccgcaggag ctcaggcacg gcggcaaact cggcgagccc
     1561 gaactgcagg cgtgggccga tgcccggctg ctgcgtgagc gtttggcgaa agtgcgtggc
     1621 cgcgccacct ttcagggttt ctccggtgtc gcgccgggca ggatcgtcga gatcaccgga
     1681 attggcgcgc ggtttgccgg tgaaatgtac gtttccggcg ttcgccatcg cctgtccggc
     1741 ggcaattggg aaaccgacgt ccaattcggt ttgagcaccg aatgctttgc cgaaacctac
     1801 aatctccgtc ctctcccggc ggccggcttg ttgccggccg tctgcgggct gcagatcggc
     1861 gtcgttacga cgctggagaa cgaccctgcc ggggaagacc ggatcaaggt ccgcatgccc
     1921 ctgatcagca gcagcgagga cggcatctgg gcacgcctcg tcactctcga cgcgggcaag
     1981 caacggggca cgtattttcg gccggaaatc ggtgatgaag tggtcgtcgg atttctgaat
     2041 gacgacccac gccacccggt ggtgctcgga atgtgtcata gcagcgccaa acctgctccc
     2101 gaagccgcca aggatgacaa ccacctcaag gggtacgtca gtcgcgagaa actgcgtctg
     2161 aactttgatg accagaagaa gatcatcggc ctggaaacac cgggaggcaa caagcttacc
     2221 ctctccgaag atgccaaagg gatatcgctg gaagaccaga atggcaaccg gattaccctg
     2281 gacgacagcg gcatcacgat cgagagtgtc aaggatcttt ccgtcaaggc cagcaaggat
     2341 ctgaagctgc agggcaccaa cacggaactc aaggcgacgg cggccttcaa ggcgtcgggc
     2401 agcagcagcg ccgagatctc gagcccgagc acgagcatca aaggcagtgc cacgacggtc
     2461 atacagggtg gcatggtcca gatcaactag ccatcgcggc gtcgggagtc gtcggctggc
     2521 gtgttgccgg gccgcgcgtt ttccaactgg agtactggca gaaagtatcg gcaaaggaaa
     2581 gcagaacatg cctgcagcag cccggattac cgacatgatc gtcagcacgg cgacgcttgg
     2641 tgtccccgtc cccatcattc cgcccggcgc cccgacggtg ttgatcggag gcttgccggc
     2701 ggcacgcctg ggagattcct gcggcgccga tgtcatcatc aaggggtcgg cgacggttct
     2761 catcggtggc atgccggccg cccgggtggg cgacccgacg gctggcggcg gggctgtcat
     2821 gcctcccggc gtcctgcagg tcaacattgg ggggtgagca gcgatgaagc aggactttct
     2881 cggtcgtggc tggtctttcc cacctgaatt tgtggccgac agggcggcag tagaaatgct
     2941 cgaggaggag gccgacatag cctccagcct cgaaatcctg ctcagcacgg cgcaaggcga
     3001 acgccttctg cagcctttgt acggttgcaa cctggacgaa ctgctattcg aatcgctgga
     3061 tacccgcatg aagacgctga tggccgacaa ggtcgagtcc gcgatcctct acaacgaacc
     3121 gcgcatcagc ctggagaggg tcaagctgga cgacagtcag gagttgcagg gactggtgtt
     3181 gatcgaggtg gtctaccggg tgaagtcgac caattcccgt ttcaactttg tctacccgta
     3241 ttacaaactg gagggcaccg atatcaacct ctcgaccacc gtcaacctcc tgccggacac
     3301 cgcctgaatc atggccacct cgaccgattg ccagcagaac caggatcccc tcaaactgat
     3361 ccgcgaaggg acgagccaga accaacggct gccggtcgcg ctggatccgg gctacgcgcc
     3421 ggtggacggg cggacgacag cacacggcat ggtcttcgcc gacagttatg ccgccttgct
     3481 gaagtatttt gacgcgacga attccgaggc tggcgactgg aaggcgtttt tcggcaacga
     3541 cgtatcggtt cgactggcgg tcgtcgccgt cgaggatgcc gacgcctaca agcgcaacat
     3601 caagtcgtat ctcgactatc tggacgaccg ccagaaccgg aacgaaggcg agcagctaag
     3661 gaaccacctg ggcttcctct acggcagcct gggcacgctg gcccggcaac tggatctcct
     3721 caaggaggcc ttgccagccg acatcgccct caaaagtacc ctgcaaaacc tgatcaagag
     3781 tcaattggct ccggcattca ggcgcttgat ggcgtattac caggcgggca acagtctggc
     3841 cctggtcaat tcggtggcgc agtcaccgga gaccacgatc ctgcgcgccc cggtgacgac
     3901 attcgcggcg gttctcactg ccggcctgtc tacggactgg agcgccggtc gtccatggga
     3961 gacctatgtg gccacagtgc ttgcagaccc tgtggtctac ggtgacgcag cggcggatgt
     4021 cttcgtgcgc atcaatcatt gcgcgacgca tgcgcttttc aaatcgattt tcgagcagtt
     4081 cctgaaggtg tttgcccgta ccgtcagtga agccgcgctg gcactggaag gtaccctgaa
     4141 caattgggac cgacaccaac cccactatgc attgttcctg gctttcctcc gtctgttgga
     4201 ctacctgcgg gcggacatca acaccttgac cagacggcat ctggatttct attaccggga
     4261 gat
//
LOCUS       DAOSIT010000157         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18346_length_4253_cov_10.433778, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000157 DAOSIT010000000
VERSION     DAOSIT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18346_length_4253_cov_10.433778"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2790
                     /locus_tag="PLL14_05095"
     CDS             <1..2790
                     /locus_tag="PLL14_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03331.1"
                     /translation="QLLAEMETLLANALVGIVHLRHRRVVSCNRRLEEIFGYLPGELI
                     GRTSECFYPSNEVFEQIGTRAYGVVAEGRNFRAELMLRRKDGSLFWGVLTGRAIDPSQ
                     PQEGSIWVYADISEQRQAEEESQNLLQAVEQSPVAIVITNRNAEIEYVNPSFTRVTGY
                     TRLEAIGQNPRILKSGLVPLETYKTLWQTLLAGKVWNGLLHNRRKNGELFWEKASIAP
                     VFGDHGEVTHYLAVKEDVTERLQAEKLLRESEEAFRHLFEDAKDPLLLLQDGHFIDCN
                     TATLELLGYPSKAEFLGRNPEDISPACQPDGQSSKEKAAVMMATAHRCGYHRFEWTHQ
                     RMDGSPVSVEVTLTPITLRGEVILHTLWRDISDRRITETRLRLLAGVFEHSAEAIMVS
                     DRDNRILEVNKAFCRLTGYSAEEVRGKDPRLLSSGRSAPEEYRAMWQSITSSGHWQGE
                     IWDRRKDGSVYPKWLSISTMRAADGDIEHYIGSFVDISERKAAEEKISHLAHFDTLTD
                     LPNRSNLQGRLDQVLASARRDGHRHPVAVMFLDLDRFKNVNDTLGHHVGDALLLEVSK
                     RLTASVRESDVVARLGGDEFVVVLTGADAIAAERVAAKVLKTLSQPCQIDGHGLHTTS
                     SIGIAVFPGDGETVEILMRNADAAMYHAKSAGRNNVQFFTASMNEAARDRRQLEEDLH
                     LALQQGQFVLHYQPQIDALQRVFGTEVLLRWQHPEHGLVAPARFIPLAEDTGLIVPIG
                     HWVLETACAQITAWATDARTRELQVAVNVSARQFRQADFVDQLRELLERSGAEPTRLK
                     LELTESLVLDNVADTIAKMHAIKLLGVTFSMDDFGTGYSSLSYLTRLPLDQLKIDRAF
                     VRRLPDNQNDAVIAQTIVTMGRSLGLKVIAEGVENEAQRQFLANHGCHGYQGFLFSRP
                     LPVEAFEQFLREKPI"
     gene            complement(2823..3884)
                     /gene="mutY"
                     /locus_tag="PLL14_05100"
     CDS             complement(2823..3884)
                     /gene="mutY"
                     /locus_tag="PLL14_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOG03332.1"
                     /translation="MKAARSSFVPSFSERVIAWQKVSGRHDLPWQQGGDAYRIWLSEV
                     MLQQTQVRSVIPYFARFLERFPTLQVLAAAPLESVLEQWSGLGYYARARNLHRCAQIL
                     VRERGGGFSAEPADNAQLPGIGRSTAAAISVFAFGSRAAILDGNVRRVLGRCFGIAGG
                     DSPARDARVLWDLAESLLPENDIETYTQGMMDLGATVCSRRKPSCDTCPLQGICVARY
                     EGRQTDFPQRPKPKVLPQRLSRLLLFCDGRRVLLERRPPSGIWGGLLSLPEWSGESAE
                     RFAENQGCRLVDTQSLPPVSHAFTHFRLTIDVLLCKVEIAGLRAMEVRWQWLPLENIA
                     AAALPMPIKKLLLTLPLQG"
BASE COUNT          902 a         1347 c         1243 g          761 t
ORIGIN      
        1 cagttgctgg ccgaaatgga aacgctcctc gccaacgcac tggtgggcat cgtacacctg
       61 cggcaccgtc gagtggtttc gtgcaaccgg cgtcttgagg agatcttcgg ctatctaccg
      121 ggtgaactga tcggcagaac ttccgaatgc ttttaccctt cgaacgaggt cttcgaacag
      181 atcggcaccc gggcttacgg cgtcgtcgcc gaaggccgaa attttcgggc cgaactgatg
      241 ctgagacgca aggatggcag cctcttctgg ggcgtgctga ccggacgggc catcgacccc
      301 agccagcccc aggaaggcag tatctgggtc tacgccgaca tctcggagca gcgtcaggcg
      361 gaggaagaat cccagaacct gctgcaggct gtggaacaaa gccccgtggc catcgtcatc
      421 accaaccgca atgccgagat cgagtacgtc aatccgagct tcacgcgcgt aaccggttat
      481 acgcggcttg aagcgatcgg ccagaacccg cgcattctga aatccggcct ggtccctctt
      541 gaaacctaca agacgctgtg gcaaaccttg ctcgcgggca aagtctggaa cggccttctc
      601 cacaaccggc gcaagaatgg cgaactgttc tgggaaaagg cctcgattgc cccggtattc
      661 ggcgatcacg gcgaggtgac gcactatctg gcagtcaagg aagacgtcac cgagcgcctg
      721 caagcggaga agctgctacg cgagagcgag gaggctttcc gccacctctt cgaagacgcc
      781 aaggaccccc tgctgctgct ccaggatgga cacttcatcg actgcaacac agccaccctg
      841 gaacttctcg gctacccttc caaggccgag tttctcggac gcaacccgga agacatctca
      901 ccggcctgcc aacccgacgg ccagtcatcg aaggagaaag ccgcagtgat gatggctacc
      961 gcacatcgct gcggctacca ccgtttcgag tggacgcacc agcgcatgga cggctcaccg
     1021 gtttcggtcg aggtcacgct gactccgatc accctgcgtg gcgaggtgat cctgcacacc
     1081 ctgtggcgcg acatcagcga ccggcgcatc accgaaacgc gcttgcgcct gctggccgga
     1141 gttttcgaac acagtgccga agcgatcatg gtcagcgacc gggacaaccg cattctggaa
     1201 gtcaacaagg ccttctgtcg cctcaccggc tacagtgccg aggaagtccg cggcaaggat
     1261 ccacgtctcc tgtcttccgg gcgctcggct ccggaagagt atcgagcgat gtggcagtcg
     1321 attaccagca gtggccactg gcaaggcgaa atctgggatc gccgcaagga tggcagcgtg
     1381 tacccgaaat ggctctcgat ctcgaccatg cgcgctgccg atggcgacat cgaacactat
     1441 atcggcagct tcgtcgacat ctcggagcgc aaggcagctg aagaaaagat cagtcatctg
     1501 gcgcatttcg acaccctgac cgatctgcct aaccgctcca acctgcaggg ccggctcgat
     1561 caggtcctgg cctcggcgcg acgagacggc catcgccacc cggtagccgt gatgttcctt
     1621 gatctcgacc gcttcaagaa tgtcaacgac actctcggtc accacgtcgg cgatgcgctg
     1681 ttgctcgaag tgtccaagag gctgacggca agcgtgcgcg agagcgacgt ggtggcacgc
     1741 cttggcggtg acgaattcgt cgtcgtattg accggggccg acgccattgc agccgagcga
     1801 gtggcggcga aagtgctcaa gaccctgtcg caaccgtgtc aaatcgacgg acatgggcta
     1861 catacgacgt caagtattgg tattgccgtc ttccccggag atggcgaaac cgtcgaaatc
     1921 ctgatgcgca atgccgacgc cgcgatgtat cacgcgaaat cggccggacg caacaacgtg
     1981 cagttcttta ccgcgagcat gaacgaagcc gccagagacc ggcgacaact cgaggaagat
     2041 ctgcatctgg ccttgcagca gggccagttt gtactgcatt accaaccgca gatcgatgcc
     2101 ctgcagcgtg ttttcggaac cgaggtgctg ctgcgttggc agcatcccga acacgggctg
     2161 gtcgcaccgg ccagattcat ccccttggcc gaagacaccg gactgatcgt gccgatcggg
     2221 cactgggtac tggaaaccgc ctgcgcacag atcaccgcgt gggcgaccga tgcccggact
     2281 cgcgagttgc aggtggcggt caacgtcagc gcacgccagt tccgtcaagc cgatttcgtc
     2341 gaccaactac gggagctact ggagcgtagc ggcgccgagc cgacacgcct gaaactcgaa
     2401 ctgaccgaga gcctggtgct ggacaacgtc gccgatacca tcgccaagat gcatgccatc
     2461 aagctgcttg gcgtaacttt ctcgatggac gacttcggca ccggctattc ttcgctttcc
     2521 tacctgaccc ggttaccgct cgaccagctc aagatcgacc gggcattcgt ccgccgcctc
     2581 ccggacaacc agaacgacgc ggtaatcgct cagaccatcg tcaccatggg acgcagcctc
     2641 ggcctcaagg tgattgccga gggggtggaa aatgaagccc aacgccagtt tcttgccaat
     2701 cacggctgcc acggctacca gggcttcctc ttcagccgcc ccttgccggt cgaagctttc
     2761 gaacagttct tgagggagaa gccgatctga ccagccagca cccaggccgg caggctgtcg
     2821 gatcaacctt gcagaggcag cgtcagcaac agcttcttga tcggcatcgg caaggccgca
     2881 gccgcgatgt tctccagagg cagccattgc caacgcactt ccattgcccg caggcctgcg
     2941 atttcgacct tgcagagcag cacatcgatg gtcagacgga agtgcgtgaa ggcgtggcta
     3001 actggcggca ggctttgtgt atccaccaga cgacagccct ggttctcggc gaaacgttcg
     3061 gcagattcgc ctgaccattc aggcaagctc aacaaaccac cccagatacc acttggtggg
     3121 cgacgttcga gcagcactcg tcggccatcg caaaacagca acaggcggct gagacgttgc
     3181 ggcagtacct ttggtttcgg gcgctgggga aagtcggttt gccgaccctc gtaccgggcg
     3241 acgcagattc cctgcaaagg acacgtgtcg caactgggct tgcgacggct gcagaccgtg
     3301 gccccgagat ccatcatccc ttgggtatag gtttcgatgt cgttttccgg gagcagcgat
     3361 tcggccagat cccacaacac gcgtgcgtcg cgtgccggcg agtcgccacc ggcgataccg
     3421 aagcaacgcc cgagcacgcg cctgacgttg ccatcgagaa tggctgcccg actgccaaag
     3481 gcaaaaacgc tgatcgccgc cgcggtggag cggccaattc ccggcagttg ggcattgtcg
     3541 gccggttcgg cagagaagcc cccgccacgc tcgcggacaa ggatttgtgc gcagcggtgc
     3601 aaatttcgtg cgcgcgcata atagccaaga ccagaccact gttccagcac gctctcgagc
     3661 ggtgctgcgg caagcacttg cagcgtcgga aagcgttcca gaaatcgagc aaaatacgga
     3721 atcacgctgc gcacctgcgt ttgctgcagc atgacctcgg atagccagat gcggtaggcg
     3781 tcgccgccct gctgccatgg caggtcgtga cgaccgctca ctttttgcca ggcgatcacc
     3841 cgttcggaga agctcgggac gaaggacgat cgagccgcct tcatccgcga ggcaacaacc
     3901 tctggcgtgc tgcccatcgg cctataaacg atgacgaggg tcgatctgca gactcacttc
     3961 agcaacctga caccaaccac ccgcaggacg ccgggccagc agaatcgtct cgacacgcgc
     4021 cggtcgcacg gcgataatca cgaacctggg ccgattgtcg gcgctccgca tccactgcaa
     4081 accgactcaa gggcatgcaa gcgctggtga tgtgctttgt gacgcactcc aaagctgcag
     4141 gtgacatcgc ggaagtctct cctttctcta tcgaccggga atcttagcat gcctcgacaa
     4201 cgtctccgga ttgcctgatg aaccttactc ttgcctctcc ggagtgcccg gaa
//
LOCUS       DAOSIT010000158         4248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18371_length_4248_cov_9.640114, whole genome shotgun sequence.
ACCESSION   DAOSIT010000158 DAOSIT010000000
VERSION     DAOSIT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18371_length_4248_cov_9.640114"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1610)
                     /locus_tag="PLL14_05105"
     CDS             complement(114..1610)
                     /locus_tag="PLL14_05105"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOG03333.1"
                     /translation="MEFSIKSGSPEKQRSACVVVGVFEPRKLTLAAELLDNAARNHLS
                     DIIRRGDMEGKSGSTLLLHNVPGTLCDRVLLVGLGKEKEFGDKEYCDAVRSAVRTLNE
                     TGSFDGTVFLTETSVRKRSAAWRVRQATIVAQETVYRFDQFKSKKDKVRRPLRKLTFV
                     VDRRNELATAEEALNQGQAIAEGMALAKNLANLPANVCTPTYLAEIAQKLAAEHKLDC
                     QILDSDEMAALGMQSLLAVAKGSQQPPRLIILQHKGGKADDKPVVLVGKGVTFDSGGI
                     SIKPAAEMDEMKYDMCGAAGVLGTIKAAAMMKLPINLTVIVPSTENMPGGAATRPGDI
                     VTSMSGQTIEILNTDAEGRLILCDALTYAARFEPDILIDVATLTGACVVALGHVATGL
                     FANNDTLARELLHAGDEAHDRAWQMPLWDDYQELLKSPFADMANIGGRWGGSITAACF
                     LSRFTRKYVWAHLDIAGTAWKSGTDKGATGRPVPLLMHYLLKRVGKLN"
     gene            1685..2764
                     /gene="lptF"
                     /locus_tag="PLL14_05110"
     CDS             1685..2764
                     /gene="lptF"
                     /locus_tag="PLL14_05110"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOG03334.1"
                     /translation="MIFQRAVRREFTHSAAGVFTALFAIMMTTQLIRLLNEAAQGSVE
                     PDAVVALLGFASLNYLPVLLSLSSFIAILLTLSRSYRDSEMVVWFSSGLPLTAWIRPV
                     LVFALPLVATIAALAFFFSPWAVSQSAEFRGKLNSRKDAGQISPGAFQESSAGDRVIF
                     VEGVAEDTNNVRNVFVSEGRPERLGVTMAGSGHQEVAENGDRFIVLQNGRRYDFQPGT
                     ADFRILEYARYAVRLETKESRGLAPTPRTMPTLDLLPIDNPQHRAELLYRVSLPFSAI
                     VLALLAIPLSFTNPRAGRSANMLFAILVYLIYNNLVTISQGWVASGKVSFAVGVLAVH
                     LLMLCLLPALFYRRIAVFSFLRWSR"
     gene            2761..3834
                     /gene="lptG"
                     /locus_tag="PLL14_05115"
     CDS             2761..3834
                     /gene="lptG"
                     /locus_tag="PLL14_05115"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOG03335.1"
                     /translation="MKVYRRYLAREVSGAILLVLLAFLALFAFFDLLGEIKSVGEGGY
                     QLHHAFAFVLLRAPGRAYELVPIAVLIGTLYALSALARHSELTVLRVSGLSTVSLLGG
                     LFQVAGWFAVATFLIGEFVAPPAEKAAYQMRLKERGRMVGQDLRSGLWVKDEQSFINV
                     RVVLPDTSLRGIRIYEFDEKAQLHSITEAAEGKHVVPDSWRLTHVVQTVLDGERAEVR
                     QLPELLWRSALSPDILSVLMVSPDRMSLRHLSTYTKHLADNHQKTNRYDIAFWKKVIY
                     PLAAFVMVALALPFGGAHYRAERVGLKIFSGVMVGILFYMLNGLFSNLGAINSWSPLL
                     SAVTPSALFLFAAATMIWWADKR"
     gene            complement(3856..>4248)
                     /locus_tag="PLL14_05120"
     CDS             complement(3856..>4248)
                     /locus_tag="PLL14_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOG03336.1"
                     /translation="ILRRLASMGYEGLLLAAVLAVLLVLPHVLLGAFAHRLASPVVVQ
                     THCFLVLLIYCLWFWSHGGQTLAMKTWRIRLVSRDGLAVRPAQALLRYLLCWPSIILG
                     GVGILWALFDRDRQFLHDRLAGTQLVMV"
BASE COUNT          717 a         1275 c         1304 g          952 t
ORIGIN      
        1 ctcggcatcc ggcgcaaaca ccagcaaggc tttcctctgc gcaaaagcct tgccgatcaa
       61 ggcggctgtg gcggcaatcc ggtctggggc gttgtggtaa aagaagactc gtgtcaattg
      121 agttttccga cgcgtttcag caaatagtgc atgagcaggg gtaccggccg tccggttgcg
      181 cccttgtcgg tgcctgactt ccaggctgtg ccggcaatgt cgaggtgagc ccagacatat
      241 ttccgggtga agcgcgagag aaagcaggcg gcggtgatgc tgccgcccca gcggccgcca
      301 atgtttgcca tgtcggcgaa gggactcttg agcaactcct ggtagtcatc ccacaagggc
      361 atttgccatg cccggtcatg cgcctcgtcg ccggcatgca gcagttcgcg agcaagtgtg
      421 tcgttgttgg cgaacagacc ggtggcgaca tggccgagag cgaccacgca ggcaccggtg
      481 agggtcgcga cgtcgatcag aatgtcgggt tcgaagcgcg cagcataggt cagggcatcg
      541 cagaggatca ggcggccttc tgcgtcggtg ttgaggatct cgatggtctg gcccgacatt
      601 gaggtgacga tatcgcccgg ccgcgtggcc gcgcccccag gcatgttttc ggtgcttggc
      661 acgattacgg tcagattgat cggcagcttc atcattgctg ccgccttgat cgtgccgagc
      721 acaccggccg cgccgcacat gtcgtacttc atctcgtcca tttcggctgc cggcttgatg
      781 gaaattccgc cactatcaaa cgtgacaccc ttgccgacca ggaccacggg cttgtcatcg
      841 gccttgccgc ccttgtgctg caggatgatc agtcttggtg gctgctgcga gcctttggcg
      901 accgccagca gcgactgcat gcccaatgcc gccatctcgt cgctgtcgag gatctggcag
      961 tccagcttgt gctccgcggc cagtttttgc gctatctccg cgaggtaggt cggggtgcac
     1021 acattggccg gcaggttggc caggttcttg gccaaagcca tgccttcggc aatcgcctgg
     1081 ccctggttga gcgcttcctc ggcggtcgcc agttcgtttc gtcgatcaac gacaaaggtg
     1141 agtttgcgca ggggtcggcg gaccttgtcc ttcttgcttt tgaactgatc gaaacgatag
     1201 acggtctcct gggcgacaat cgtcgcctgg cgcacgcgcc aggccgcgct gcgcttcctg
     1261 acgctggttt cggtcaggaa aacggttccg tcgaaggagc ccgtttcgtt gagggtacgt
     1321 acggcgctgc ggactgcgtc gcagtattcc ttgtcgccga actctttttc cttgcccagt
     1381 ccgaccagca ggacgcggtc gcaaagagtt cccggaacat tgtgcagcag cagcgtgctg
     1441 ccggactttc cctccatgtc gccgcggcga atgatatcgg aaagatggtt gcgcgcggcg
     1501 ttgtcgagca actcggcggc cagtgtcaac ttgcggggtt cgaaaacgcc taccacgaca
     1561 caggcgctgc gttgcttttc cgggctcccg ctttttatgc taaattccac ttacccctcc
     1621 tcaggcaacg cgtgcttctt tcgaagccct gattatcact gcagtttttc accaaagtca
     1681 aagaatgatc tttcagcgtg cggtccgacg cgagttcacc cactctgccg ccggggtctt
     1741 cactgcgctt ttcgccatca tgatgaccac gcagttgatt cgcctgctca atgaagcggc
     1801 gcaaggcagc gtcgagccgg atgcggtggt ggcgctgctc ggcttcgcgt cgctcaacta
     1861 cctgccggtg ttgctgtcgc tgtcgtcatt tatcgctatc ctgttaaccc tgtctcgcag
     1921 ctatcgtgat tccgagatgg tcgtctggtt ctcgtccgga ttgccgctga cggcgtggat
     1981 cagaccggta ctggtctttg ccttgccgct ggtagcgacc atcgccgctc ttgccttctt
     2041 cttttcccct tgggccgtgt cccagagcgc cgagtttcgt ggcaaactga attcgcgaaa
     2101 ggacgccggg cagatctctc cgggcgcgtt tcaggaatca tcggctggcg accgcgtgat
     2161 cttcgttgaa ggcgtcgcgg aggataccaa caacgtccgc aacgtgtttg tcagcgaagg
     2221 tcgccccgaa cggctggggg tgacgatggc gggcagcggt catcaggaag tcgcggaaaa
     2281 tggcgaccgc tttatcgttc tgcagaacgg tcgccgctac gacttccagc ccggcacggc
     2341 cgatttccgc atcctcgaat acgcacgcta cgctgtccgc ctggagacca aggagtcgcg
     2401 cggtttggcg ccgacaccga ggaccatgcc caccctggac ctgctgccga tcgataatcc
     2461 ccagcatcgc gccgaattgt tgtaccgcgt gagcctgccg ttttcggcaa tcgtgctcgc
     2521 cttgctggcg atcccgctgt cgtttaccaa tccacgtgcc ggtcgttcgg cgaacatgct
     2581 gttcgccatt ctggtctacc tgatttacaa caatctggtg accatcagcc aggggtgggt
     2641 ggcgagtggc aaggtgtcct ttgcggtcgg cgtgctggcc gttcatctgc tcatgctgtg
     2701 cctccttcct gcgcttttct atcgccgcat cgccgttttc tccttccttc gctggtcgcg
     2761 atgaaggtct accgtcgcta ccttgcgcgc gaggtgtcag gcgccattct gctggtgttg
     2821 ctggcttttc tggccctgtt cgccttcttc gatctgcttg gggaaatcaa gagtgtcggc
     2881 gagggcggct atcagttgca ccatgctttt gccttcgtcc tgctgcgcgc gccgggacgg
     2941 gcctacgaac tggtgccgat tgccgtcctg atcggcacgc tttacgcttt gtcggcgctg
     3001 gcccgtcatt ccgaactgac cgtgttgcgc gtctccggtt tgtccacggt gtccttgctg
     3061 ggggggctat tccaggtggc gggctggttt gccgtagcga ctttcctgat cggtgagttt
     3121 gtcgcgccgc ctgcggagaa ggcggcgtat cagatgcgcc tgaaggaacg agggcgcatg
     3181 gttgggcagg acctgcgctc cggtctgtgg gtcaaggacg aacagagttt tatcaacgta
     3241 cgcgtcgtcc tgccggatac cagtctgcgt ggcatccgca tctacgaatt tgacgagaag
     3301 gcgcagttgc actcgatcac cgaggctgca gaggggaagc atgtggtgcc cgacagttgg
     3361 cggctgacgc atgtggtgca gacggtgctc gatggcgaac gtgccgaagt ccggcagttg
     3421 cccgaactct tgtggcgttc ggcgctgagc ccggacattc tctcggtcct gatggtttca
     3481 cccgatcgca tgtcactgcg gcatctatcg acgtatacca aacatctggc ggataaccat
     3541 cagaagacca accggtacga tatcgccttc tggaagaaag tgatctaccc gttggcagcc
     3601 ttcgtcatgg tcgccctcgc tttgccgttt ggcggtgccc attaccgggc cgagcgcgtt
     3661 ggcctcaaga tcttttccgg agtcatggtc ggcatcctgt tctacatgct caacggcctg
     3721 ttttccaatc tcggcgcgat caacagttgg tcaccgctgc tcagtgcggt gacgccttcg
     3781 gcgctgttcc tgtttgcagc cgcgacgatg atctggtggg ctgacaagcg ctgacgctga
     3841 cccgaatgga tcctctcaga ccatgaccag ttgcgtgccc gccaggcgat cgtggagaaa
     3901 ttgtcggtcg cgatcgaaca gtgcccagag gattccgaca cctcccagga tgatgctggg
     3961 ccagcacagg aggtagcgca gtaacgcctg cgccgggcgg acggccagac catcccggct
     4021 gaccaggcgg atgcgccagg ttttcatggc cagcgtctgc ccgccatggc tccagaacca
     4081 gaggcagtag atcagcagta ccaggaagca atgggtctgt accacaacgg gtgacgccag
     4141 gcgatgcgcg aaggcgccaa gcagcacgtg cggcaggacc aggagaaccg cgagcactgc
     4201 cgccaacaac agtccttcgt agcccatgct ggcaagacga cgcaggat
//
LOCUS       DAOSIT010000159         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18475_length_4232_cov_8.433565, whole genome shotgun sequence.
ACCESSION   DAOSIT010000159 DAOSIT010000000
VERSION     DAOSIT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18475_length_4232_cov_8.433565"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLL14_05125"
     CDS             complement(<1..461)
                     /locus_tag="PLL14_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOG03337.1"
                     /translation="MNSVAGHLVFDPQIIRRFDINGPRYTSYPTADRFVEAFGAEAAR
                     LWLGKRNIGAISRQLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLKYLARELALQ
                     SSYLEGGDQEVAQLHWGGGTPTFLSHDEMRQLMTATRQHFRLIDGGEYSIEV"
     gene            565..1302
                     /gene="fnr"
                     /locus_tag="PLL14_05130"
     CDS             565..1302
                     /gene="fnr"
                     /locus_tag="PLL14_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOG03338.1"
                     /translation="MNAVTARLAFEVVKTACSNCNLRELCLPIGLERDELKRLDELVS
                     TRKRLKRGDHLYRAGQGFESIYAIRSGFFKTDVMIEDGRDQVTGFQMTGELLGLDGIS
                     SEIHTCNAIALEDSEVCAIPFLQLEGLSRQIHTLQHHFHKVMSREIVRDHGVMMLLGT
                     MRAEERLAAFLLNLSQRFKARGYSPAEFNLRMSRDEIGSYLGLKLETVSRAFSRFQEE
                     GLLAVHQRKVRILNTAGLRKLLTHQRA"
     gene            complement(1299..1727)
                     /locus_tag="PLL14_05135"
     CDS             complement(1299..1727)
                     /locus_tag="PLL14_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HOG03339.1"
                     /translation="MISYLYLKYLHMGCVALSGAGFLLRGVWMLTDSPCLQQRAVRVL
                     PHLIDTILLGSAIAMAVISAQYPFAVNWLTAKLVGLLIYIGCGTMALKRARSKAQRAV
                     FLVAALGAFAYIVSAALARSPLGFFAWLAEAASATLPAAA"
     gene            complement(1724..3043)
                     /locus_tag="PLL14_05140"
     CDS             complement(1724..3043)
                     /locus_tag="PLL14_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03340.1"
                     /translation="MQALLSFDQAPPLAAPLRFFLTAPLFAILAGLLLLWNGPDLFAS
                     RWTPAALALTHLISVGFMLQVMLGAMIQILPVAAGANVSRPLLVACMVHVVITLGALL
                     LATGLLSYEPLVLRLAVFLLGSGVALFVGAAARALYGVPSTNPTVRGLKLALSGLSAT
                     VGLGLLLAIAFSGGLDLPLPLRQLTPIHLGWGFVGWGVLLLAAVAFVVVPMFQMTPPY
                     PATFAGAFSWSVLALLCMWSIAAGAAWHWPSAVLEGAVVLSVASFAALTLHVQSRSKR
                     AKFDATQHYWRVGMLCALATCVLWFAAAVVPALAERPEWSLCCGVLALVGGFMSVMIG
                     MLYKIVPFLVWLHLQNRGGGRVLAPTMNKVIATRQIDRQMYAHLLALALLLAAVVWPL
                     WFSYPAGLAFVLANAWLLHNLLTAAAFYRRHCLKIEAWEAATATPVG"
     gene            complement(3043..3279)
                     /locus_tag="PLL14_05145"
     CDS             complement(3043..3279)
                     /locus_tag="PLL14_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HOG03341.1"
                     /translation="MTKENADIVLDVCGLEPPEPLVRTMEALDQLGPAEKLLVLLPRE
                     PYPLYRALELNGFCWQTVCRPDGTVEVLIQHRQP"
     gene            complement(3276..3737)
                     /locus_tag="PLL14_05150"
     CDS             complement(3276..3737)
                     /locus_tag="PLL14_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG03342.1"
                     /translation="MHAITEFMVNDHRSCDGLLVAVEHAVANAAWATARTGFAHFSDA
                     MRCHFAAEERVLFPLFEQSTGMYHGPTQVMRAEHAQMLQLLASAASALEAADSDDYAG
                     SAETLLIMMQQHNLKEENVLYPMCDQHLADQLDALMPELQRHIAGQDGSSK"
     gene            3991..>4232
                     /locus_tag="PLL14_05155"
     CDS             3991..>4232
                     /locus_tag="PLL14_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG03343.1"
                     /translation="MFKNILVPTDGSELSRETARRAVSFAKEAGARITAFFAKPEYPV
                     SYYGEGALIDPTTPEKFAELAEQQAQQTLDFVVQLC"
BASE COUNT          892 a         1259 c         1305 g          776 t
ORIGIN      
        1 acctcgatcg agtactcgcc gccgtcgatc aatctgaagt gctggcgtgt cgccgtcatc
       61 agctgacgca tctcgtcgtg tgacaggaag gtcggcgtac ccccacccca gtgcagttgc
      121 gcgacttcct ggtcgccgcc ctcaagatag ctgctctgca atgccagttc gcgtgccaga
      181 tatttcaggt acttggccga acgaccgtgg tccttggtaa tgatcttgtt gcacgcgcag
      241 taataacaga tggtgttgca gaatggaatg tggaagtaca atgacagttg ccggctgatg
      301 gcgccgatat tccgcttgcc gagccagagc ctggcggcct ccgcaccaaa ggcttcgacg
      361 aagcggtccg cggtcggata cgacgtgtag cgcggaccgt tgatgtcgaa acggcggatg
      421 atctgtggat caaaaacgag gtggccagcg acagagttca tgttcccgct acactgttta
      481 tggttggtat tatcggaccg cgctggagca accgggttga cctggatcaa aggcagggtt
      541 taatgaaaaa ggacgcgcat gacgatgaat gctgtgacgg caaggctggc atttgaagtc
      601 gtgaagaccg cttgttcgaa ctgcaacctg cgcgagctgt gcctgcccat tggacttgag
      661 cgagacgagc tgaaaaggct ggacgaactg gtttcgacgc gcaagcgcct gaaacgcggc
      721 gatcatctct atcgtgccgg gcagggtttc gaatcgatct acgccattcg cagcggcttt
      781 ttcaagaccg atgtgatgat cgaggacggt cgtgatcagg tcaccggttt tcagatgacg
      841 ggcgagttgc tcgggctgga cggcatcagc agcgagatcc acacctgtaa cgccatcgcg
      901 ctcgaagaca gcgaagtgtg tgcgattccc ttcctgcaac tcgaagggtt atcgcggcaa
      961 atccatacct tgcagcatca cttccataag gtgatgagcc gcgagatcgt tcgcgatcat
     1021 ggggtgatga tgctgcttgg cacgatgcgc gccgaggaac gcctggcagc ctttctcctc
     1081 aatctttcgc agcgtttcaa ggcgcgtggc tattcaccgg cggaattcaa tctgcgcatg
     1141 tcgcgtgacg aaatcggcag ctatctcggt ctcaaactcg aaaccgtcag ccgggctttc
     1201 tcgcgtttcc aggaagaagg gttgttggcc gtgcatcaac gaaaggtccg tattctcaat
     1261 accgccggtt tgcgaaagct gctgacgcac cagcgcgcct aagcggcggc aggaagcgtg
     1321 gcgctcgccg cctccgcgag ccaggcgaaa aagcccagcg ggctgcgggc aagcgcggct
     1381 gatacgatgt aggcgaaggc gcccaacgca gcgaccagga acactgcccg ttgtgccttg
     1441 ctgcgggcgc gcttgagagc cattgttccg caaccgatgt agatcagcag accgacgagt
     1501 ttggcggtca accagttcac cgcaaacgga tattgggcgc tgattaccgc catggcgatc
     1561 gcgctgccga gcaggattgt atcgatcagg tgtggcagga ctcgtaccgc tcgctgctgc
     1621 agacagggcg aatcggtcag catccagacc ccgcgcaaca ggaagccggc gccgctgagc
     1681 gcaacgcagc ccatgtgcag gtacttcagg tacaggtaac tgatcatccg accggcgtgg
     1741 ccgtggcggc ttcccaggcc tcgattttca ggcaatgccg ccggtagaac gcggcggcag
     1801 tcagcagatt gtgcagcagc caggcattgg cgagcacgaa cgccaggcct gccggatagc
     1861 tgaaccacag cggccagacg actgccgcca gcaggagcgc aagcgcaagc aggtgcgcat
     1921 acatttgccg gtcgatctgt cgtgttgcga tgaccttgtt cattgtcgga gccaacactc
     1981 gcccgccacc acggttctgc aggtgcagcc agaccagaaa aggaacgatc ttgtacagca
     2041 tgccgatcat caccgacatg aagccgccaa ccagcgccag taccccgcag catagcgacc
     2101 attccgggcg ctcggcgagc gccggcacga ctgccgccgc gaaccacagg acgcaggttg
     2161 ccagggcgca cagcatgccg acacgccagt agtgctgggt ggcgtcgaac ttcgcgcgct
     2221 tgctgcggct ctggacgtgc aaggtcaggg ccgcaaagct ggccaccgac agtaccacgg
     2281 caccctccag aacggcagac ggccaatgcc aggccgcgcc ggcagcgata ctccacatgc
     2341 acagcaacgc cagcaccgac caggaaaatg cgccggcgaa cgtggcgggg tacggtggtg
     2401 tcatctggaa catcggaacc acgacgaaag cgacagcggc aagcaacagc acaccccagc
     2461 cgacgaagcc ccagccgagg tgaatgggtg tcaactgtcg caatggcagc ggcaggtcga
     2521 gcccaccgct gaaggcgatg gccagcagca ggccgaggcc gacggtggcg ctcagaccag
     2581 acagagccag tttgaggcca cgcacggtcg ggttcgtcga cggtacaccg tacaaggcgc
     2641 gtgcggctgc acccacgaac agcgccacgc cgctgccgag caggaacacg gccaacctca
     2701 gaacgagagg ttcgtagctc agcaaacccg ttgccagcaa caatgctccc agcgtgatca
     2761 cgacatggac catgcacgct accagcagcg gtcgcgacac gttcgctccg gcggctaccg
     2821 gcagaatctg tatcatcgcc cccagcatca cctgcagcat gaagccgacg ctgatcaggt
     2881 gcgtcagggc cagcgccgca ggagtccagc gcgaggcaaa caggtccggg ccgttccaca
     2941 acagcagcag gccggcgaga atggcgaata gcggcgcggt caggaagaaa cgcaacggcg
     3001 ccgccagcgg cggcgcctga tcgaaggaca gtagcgcctg cactatggct gtcgatgctg
     3061 gatcaggact tccacggtcc cgtcgggtcg gcacaccgtt tgccaacaga agccgttgag
     3121 ttcgagcgcc cggtagagtg ggtagggttc gcgcgggagg agtaccagga gcttttccgc
     3181 cggaccgagc tggtcgagtg cttccatggt acgcaccagc ggttcgggag gctccagtcc
     3241 acacacgtcg agcacgatgt cggcattttc ctttgtcatt tcgaacttcc atcctggccg
     3301 gcaatatgcc tctgcaactc gggcatcagt gcatcgagtt ggtcagccag atgctggtcg
     3361 cacatcggat acagcacgtt ctcttccttg aggttgtgtt gctgcatcat gatcaacagc
     3421 gtttctgcgc taccggcgta atcgtcgctg tctgccgcct ccagagccga cgcggcgctg
     3481 gccagcaact gcagcatttg cgcatgctcg gcgcgcatga cctgcgtcgg cccgtggtac
     3541 atgccggtac tctgttcaaa aagcgggaag agaacgcgtt cctcggccgc gaaatgacag
     3601 cgcatcgcgt cgctgaagtg ggcgaaaccg gttcgggctg tagcccacgc ggcgttcgcc
     3661 acggcgtgct cgacggcgac cagcaaaccg tcgcaggagc gatgatcgtt gaccatgaat
     3721 tccgtaatcg cgtgcatgcg gtagctctca ggagacagga ttgccattgt cgaacgggcg
     3781 gcgaccaccc acgttgaccg atatcaagta attcttatgg acggatgccg gttttccggc
     3841 gcgcatcggt gttcgggtgg ctggggatgg ttcaacaccg tcgtggttgc cgagcgtttg
     3901 tgcgcctcca ccttcaatcc tttgatgttg atcaacagtc gggccggctt tcatcctata
     3961 gttaattcat cgtcctgtgg aggaaacgcc atgttcaaga atatccttgt tccgaccgat
     4021 ggttccgaac tttccaggga aaccgcccgt cgcgcggttt cctttgccaa ggaggcgggg
     4081 gcgcgcatca cggcattctt tgccaaaccc gaatatccgg tcagctacta tggtgaaggg
     4141 gcattgatcg accccacgac gccggagaag ttcgccgaac ttgccgaaca gcaggcacaa
     4201 cagacgctgg attttgtcgt ccagttgtgt ga
//
LOCUS       DAOSIT010000160         4225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18503_length_4225_cov_11.410791, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000160 DAOSIT010000000
VERSION     DAOSIT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18503_length_4225_cov_11.410791"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLL14_05160"
     CDS             <1..282
                     /locus_tag="PLL14_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03344.1"
                     /translation="IEACDGRDALSKLGGQKIHLVISDVNMPNLDGISFVKEMKKNPA
                     YRFVPVIMLTTEGNEEKKKAGQEAGARAWVVKPFQPQQMLAAVSRLLMI"
     gene            290..619
                     /locus_tag="PLL14_05165"
     CDS             290..619
                     /locus_tag="PLL14_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG03345.1"
                     /translation="MSLALARHGQTARLTLAGELTIYSVAETKAGLADAMRSADDIEV
                     DLAGLTEIDTAGLQLMLLAKRRPGKNVRFINHPACVLRLIDLAHLGRVLGDPLYLPAS
                     EATARTP"
     gene            616..2877
                     /locus_tag="PLL14_05170"
     CDS             616..2877
                     /locus_tag="PLL14_05170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOG03346.1"
                     /translation="MNDAATDLAADLAQALGTFMEEAAELLAEMEAILLRAEAGRCRE
                     DDLNALFRCAHTLKGSGGLFGLDAVVRFTHVVENVLDRLRNGKLQFDSALISLLLDCQ
                     DHIAQLIAAVAEHTTVPLAGSDPLLARLQSVLDNGARPPTVGADPSPAGEISASGGGA
                     FGADHWHISLRPGKQVLQDGMDPLAFIHYLSQYGQILHVETLTSGLPEFTAADPELCH
                     LGFEVALASQASKAEIEGVFDFLREGSQIHILPPQSQIAEYLALIEAHHHDDVERIGE
                     MLVACGSITAHELTTALAAQPHQAPRRLGEILVEQGAVPPRVVAAAADKQQRAEARRS
                     SDARLVKVPADRLDALIDRVGELVIAGVGTHLQVSRLQRPDLQESAETLLSLVQDIRD
                     MTLRLRMVAIGEVFARFPRVVRDLSRELGKDIELAIRGAETELDKSMVDRIGDPLMHL
                     VRNAIDHGIESAETRQARGKPARGTVGLHAYHESGSITIEVSDDGGGLDRERILQTAI
                     QKGLLPPDAALSDQEVYRLILAPGFSTAEKVTELSGRGVGMDVVRSNIEALRGTIDID
                     SVPGQSTTMRLCLPLTLAIIDGFHVGVGGSHFIIPLDMVVECLELPPEIGERQYLELR
                     DQILPFVRLRQLFDEPGPQHPRPRVIVVRYGHRQAGLVVDRIHGQCQTVIKPLGPLFA
                     GVPAVSGCTIIGNGEVGMILDVPALVRQCVAAGRRPRRLATSPPGGVAQGANARVIDA
                     RAAISDDSMGSGT"
     gene            2874..>4225
                     /locus_tag="PLL14_05175"
     CDS             2874..>4225
                     /locus_tag="PLL14_05175"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG03347.1"
                     /translation="MNGLRSITVGKRNLIWGGVTAIGLILFCIVSLITIRDGMMEDRK
                     QRIKSLVEAGTGVLDHYQQLSASGKLSEKDARHTALEALRHMRYEGGNYLFGFDPEGN
                     YYLSPASRDFEGTNKIDLKDANGKYLIRELIQAAKAGGGYVQYDFPKPGMQSASPKIG
                     YAALFTPWNLVIGTGIYIDDVDAVFRRVLIQLGSISLALLALLTALGWAISRSISVPL
                     NAVVDVSKRMAAGDFAFTLDTAGRDEAGAVFRAVTLVQEAVRAMLGDARSLSAAAAQG
                     NLAARADAAAHQGEFRAIIEGLNSTLDAVVGPVNAVKRVMAAVEQGDLSESIDADYQG
                     DFGALQASVNNTVGKLSATIAQVNTSASELSNAATQVSATSQVLSQATSEQAASLEQT
                     TSAMEQMSASIAQNTDNAKTTDGIAGQSAADALAGGEAVRSTVAAMKSIAGKISIIDD
                     "
BASE COUNT          807 a         1594 c         1236 g          588 t
ORIGIN      
        1 atcgaagcct gcgacggacg ggacgcgctg agcaagctgg ggggacagaa gatccacctg
       61 gtgatcagcg acgtgaacat gccgaacctg gatggcatca gcttcgtcaa ggagatgaag
      121 aagaaccccg cctaccggtt cgtgccggtg atcatgctca ccaccgaagg caacgaagag
      181 aagaagaaag cgggccagga agccggcgcc agagcctggg tagtcaagcc cttccagccg
      241 cagcagatgc tcgcggcggt atcccgactg ttgatgatct gaagcggcga tgagcctcgc
      301 cctagcccgc cacggccaga ccgcgcggct gaccctcgcc ggcgaactca ccatctacag
      361 cgttgcagaa accaaggccg gactggccga cgccatgcgc agcgccgacg acatcgaagt
      421 cgacctcgcc ggcctcaccg aaatcgacac cgccggactg caactgatgc tcctcgccaa
      481 acgccgcccc ggcaagaacg tccgcttcat caaccacccc gcgtgtgtcc tgcgcctgat
      541 cgacctcgcc catctcggcc gcgtgctcgg cgaccccctg tacctccccg ccagcgaagc
      601 caccgcgcgc accccatgaa cgacgccgcc acggacctcg ccgccgacct cgcccaggcc
      661 ctcggcacct tcatggaaga agccgccgaa ctcctcgccg aaatggaagc gatcctgctg
      721 cgtgccgaag ccggtcgctg ccgcgaagac gacctcaacg ccctgtttcg ctgcgcacac
      781 accctcaaag gctccggcgg actgttcggc ctcgacgccg tcgtccgctt cacgcacgtc
      841 gtcgaaaacg tcctcgaccg cctgcgcaac ggcaaactcc agttcgacag cgcactgatc
      901 agcctgctgc tcgactgtca ggaccacatc gcccaactga tcgcggccgt cgccgaacac
      961 accaccgtcc ccctcgccgg cagcgacccc ctgctcgccc gcctgcaaag cgtcctcgat
     1021 aacggcgccc gcccacccac cgtcggcgcc gacccgtcgc ccgccggcga aatcagcgcc
     1081 agcggcggtg gcgcattcgg cgccgaccac tggcatatct cgctgcgccc cggcaaacag
     1141 gtcctgcagg acggcatgga cccgctggcc ttcatccact acctgagcca gtacggccag
     1201 atcctgcacg tcgaaaccct caccagcggc ctgcccgagt tcaccgccgc ggatcccgaa
     1261 ctctgccacc tcggattcga agtcgccctc gccagccagg ccagcaaggc cgaaatcgaa
     1321 ggcgtcttcg acttcctccg cgaaggctcg cagatccaca tcctcccccc gcagagccag
     1381 atcgccgagt acctcgcgct catcgaagcc caccaccacg acgacgtcga gcgcatcggc
     1441 gaaatgctcg tcgcctgcgg atcgatcacc gcccacgaac tgaccaccgc actcgccgcg
     1501 cagcctcacc aggccccgcg gcgcctcggc gagatcctcg tcgaacaagg cgccgtaccg
     1561 ccgcgggttg tcgccgccgc ggccgacaaa caacagcgcg ccgaagccag gcgcagcagc
     1621 gacgcacgcc tcgtcaaagt cccggcggac cgcctggacg ccctgatcga ccgcgtcggc
     1681 gaactggtca tcgccggcgt cggaacccac ctgcaggtca gccgcctgca acggcccgac
     1741 ctccaggaat cggccgaaac cctgctctcg ctcgtccagg acatccgcga catgaccctg
     1801 cgcctgcgca tggtcgccat cggcgaagtc ttcgcccgct ttccacgcgt cgttcgcgac
     1861 ctctcccggg aattgggcaa agacatcgaa ctcgccatcc gcggcgccga aaccgaactc
     1921 gacaaatcga tggtcgaccg gatcggcgac cccctcatgc acctggtccg caacgccatc
     1981 gaccacggca tcgagtccgc cgaaacacgc caggcacgcg gcaaacccgc gcgcggcacc
     2041 gtcggactcc acgcctacca cgagtccggc agcatcacca tcgaagtcag cgacgacggc
     2101 ggcggcctcg accgcgaacg catcctccag accgcgatcc agaaaggcct gctccccccc
     2161 gacgccgcac tgagcgacca ggaagtctat cggctgattc tcgcgccggg cttctccacc
     2221 gccgaaaaag tcaccgaact ctccggccgc ggcgtcggca tggacgtcgt acgcagcaat
     2281 atcgaagccc tgcggggcac gatcgacatc gattccgtgc ccgggcagag caccaccatg
     2341 cgcctgtgcc tgccgctgac gctggcgatc atcgacggct tccatgtcgg cgtcggcggc
     2401 tcgcacttca tcatcccgct cgacatggtc gtcgagtgcc tcgaactccc ccccgaaatc
     2461 ggcgaacgcc aatacctcga actgcgcgac cagatcctgc ccttcgttcg cctgcgccaa
     2521 ctcttcgacg aaccggggcc gcaacaccca cgcccgcgcg tcatcgtcgt ccgatacggc
     2581 caccgccagg ccggcctggt cgtcgatcgc atccatggcc aatgccagac cgtgatcaaa
     2641 ccgctcggcc ccctgttcgc cggcgtgccg gccgtctcgg ggtgcaccat catcggcaac
     2701 ggcgaagtcg gcatgattct cgacgtcccg gccctggtcc gacagtgcgt cgccgccggc
     2761 cgacgaccgc gccgcctggc gacgtccccg ccaggcggcg tcgcgcaagg agcaaacgcc
     2821 cgcgtcatcg acgcccgggc agcgatttca gacgattcaa tggggagtgg aacatgaacg
     2881 gattacgctc gatcacggtc ggcaagcgca acctcatctg gggaggggtc acggccatcg
     2941 gcctgatcct cttctgtatc gtgtcgctga tcaccatccg cgacggcatg atggaagacc
     3001 gcaagcaacg gatcaagagc ctggtcgagg ccggcaccgg cgtgctcgac cactaccagc
     3061 aactctcggc gagcggcaag ctcagcgaaa aggacgcccg ccacaccgcg ctcgaagcct
     3121 tgcgccacat gcgctacgaa ggcggcaact acctgttcgg cttcgacccc gaaggcaact
     3181 actacctgag cccggccagc cgcgacttcg aaggcaccaa caagatcgac ctcaaggacg
     3241 ccaacggcaa atacctgatc cgggaactga tccaggcggc caaggccggc ggcggctacg
     3301 tccagtacga cttccccaaa ccgggcatgc agtcggcgtc gccgaagatc ggctacgccg
     3361 ccctcttcac gccgtggaat ctggtgatcg gcaccggcat ctacatcgac gacgtcgatg
     3421 ccgtcttccg tcgcgtcctg atccagctgg gctcgatttc cctggcgctg ctggcgctgc
     3481 tgaccgccct gggctgggcg atctcgcgct cgatctccgt accgctcaat gcggtcgtcg
     3541 acgtctccaa gcgcatggct gccggcgact tcgcgtttac gctcgacacc gccggacgcg
     3601 atgaagccgg cgccgtcttc cgggcggtga cgctggtcca ggaagcggtg cgggcgatgc
     3661 tcggcgatgc ccgctccctg tcggcggcgg cggcgcaagg caacctggcg gcgcgcgccg
     3721 atgcggccgc gcaccagggc gaattccggg ccatcatcga aggcctcaac agcaccctgg
     3781 atgccgtggt cggcccggtc aacgcggtca agcgggtgat ggcggcggtc gagcagggcg
     3841 atctgagcga aagcatcgac gccgactacc aaggcgactt tggcgccctg caggcgagcg
     3901 tgaataacac cgtcggcaaa ctgtcggcga ccatcgcgca ggtgaatacc tcggccagcg
     3961 aactgtcgaa cgccgcgacg caggtctcgg cgacctcgca ggtcctgtcg caggccacct
     4021 ccgagcaggc ggcgagcctc gaacaaacga cctcggcgat ggagcagatg tcggcgtcga
     4081 ttgcccagaa caccgacaac gccaagacca ccgacggcat cgccgggcaa tcggcggcgg
     4141 acgcgctggc cggcggcgaa gccgtgcgct cgacggtcgc ggcgatgaaa tcgatcgccg
     4201 gcaagatctc gatcatcgac gacat
//
LOCUS       DAOSIT010000161         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18687_length_4195_cov_11.246135, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000161 DAOSIT010000000
VERSION     DAOSIT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18687_length_4195_cov_11.246135"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLL14_05180"
     CDS             complement(<1..266)
                     /locus_tag="PLL14_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768303.1"
                     /note="catalyze the formation of cyanophycin which may act
                     to store excess nitrogen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG03348.1"
                     /translation="MQVSRIRALRGPNLWSRHTAIEAIVYCAEDERSIDSIPGFETRL
                     RERFPELAMLGPAGHDEAVSMAQALEFAALGLQAQAGCPVTFSRT"
     gene            complement(335..2506)
                     /gene="cphA"
                     /locus_tag="PLL14_05185"
     CDS             complement(335..2506)
                     /gene="cphA"
                     /locus_tag="PLL14_05185"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG03349.1"
                     /translation="MKSKDIKFLEIRYLRGPNIWTYRPVIEALVDIGELEDFPSNTIP
                     GFHERLIALLPSLIEHRCSYGERGGFLRRVEEGTWAAHMLEHVTLELQNLAGMPGGFG
                     KARESSTRGVYRVVVRAWHEDVTRSCLYAGRDLLLAAIKDQTFDVAGTVERLADMAER
                     KLLGPSTGCIVEAATAKDRRIPAIRLLATGNLVQLGYGARSRRIWTAETDRTSAIAEG
                     ISRDKDLTKTLLKSCGVPVPEGRLVDSAEDAWDAAEEIGLPVVVKPSDGNHARGVFTN
                     LMAREEVESAYAAAVVEGSGVIVERYVRGSEHRLLIVGGKLAAAARGETAKVVGDGQS
                     TIDELIDSQINSDPRRGAAEEFPLDIIALADNPVARLEVSRQGFAPDSVPAAGREVLI
                     VRSGNHTDDVTDLVHPETAATASLAARIVGLDIAGVDLVCEDISQPLDGQRGGIVEVN
                     AGPGLLMHLKPETGQPRPVGRAIVDELFPNGDDGRIPVVGVTGSFGKTTVARLIASLL
                     TLSGRHTGLASSDGLFVHRRCIAQGDNANWGGAHRLLMNRAVEAAVFENGTDSILSEG
                     LAYDRCQVGVITNVEAARHFGRYHVETPEQVFTVLRTQVDLVLPTGAAVLNASQPMLV
                     EIAPLCDGEVIFFGLDAALPTLVEHLAQGKRAVFVRNERVVLANGANETEIASLKGIP
                     LTNGGRTDYQIENVLAASGAAWALGIGPEIIRTGLQTFTIA"
     gene            complement(2658..3131)
                     /locus_tag="PLL14_05190"
     CDS             complement(2658..3131)
                     /locus_tag="PLL14_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOG03350.1"
                     /translation="MTIDPAYQLRRNAFGKLEFIGADGEVHAVVVPVRAFPITDPDDG
                     IGLVDPYGHELAWIDRLDALPDHLRILVESELAQREFMPEITRIVGVASFATPSTWQV
                     ETDRGAASFVLKGEEDIRRLTSPALLIADSHGIHFLIRDRYALDQHSRRILDRFL"
     gene            complement(3128..>4195)
                     /locus_tag="PLL14_05195"
     CDS             complement(3128..>4195)
                     /locus_tag="PLL14_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03351.1"
                     /translation="WSVFSPTVTLLTEIGLLIVWAFGIWQIAKDQITVGVLTAFLAYI
                     SRFYLRLDSMSRIVSVTQKAAAGAKRIFDILDHVSSVPEPTHPVHLPKVSGRIELRGV
                     GFRYGTRSVTRDINIVIRPGEMVGLVGHSGSGKSTLVNLICRFYDVTEGAILIDGVDI
                     RSLPVAEYRKHIGLVLQEPFLFFGTIAENIAYGKPDATHAEIVAAARAAHAHEFILRL
                     PHGYDSLVGERGQALSGGERQRISIARALLIDPRILILDEATSSVDTTTEKEIQKALD
                     NLVRGRTTIAIAHRLSTLRDANRLVVLDRGSVVEVGSHDELMACEGHYYRLYQAQARN
                     VDTEDELRRMELARHDEEDRQ"
BASE COUNT          723 a         1273 c         1415 g          784 t
ORIGIN      
        1 gtgcggctga aggtgaccgg acagccggcc tgagcctgca ggccgagcgc agcgaactcg
       61 agcgcctgcg ccatcgatac ggcttcatca tgaccggcag gcccgagcat ggccaactcg
      121 ggaaagcgct cacgcaaacg ggtttcgaaa cccgggatgc tgtcgatgct gcgttcgtcc
      181 tcggcgcagt agacaatggc ctcgatcgcc gtgtggcggc tccacagatt gggaccgcgc
      241 agagcgcgaa ttcgcgatac ttgcatggac agtttcctct ttcgggttca gtgcggacgc
      301 cggtcggtgg cgctggctcg ttgcggtcca cgactcaggc gatggtgaac gtctgcagcc
      361 cggtacgtat gatttccggc ccaatgccca gcgcccacgc ggcgccgctg gcggcaagga
      421 cattctcgat ctggtaatcg gtgcgcccac cgttggtcag tgggatgccc ttcaggctgg
      481 cgatctccgt ttcgttggcg ccgttggcca gtacgacccg ctcgttgcgt acgaataccg
      541 cccgtttgcc ttgggccagg tgttcgacaa gggtgggcag agcggcgtcc aggccaaaga
      601 agatcacctc gccgtcgcac aatggcgcga tctcgaccag catcggctgg cttgcattga
      661 gtaccgcggc acccgtgggc aataccagat cgacctgcgt gcgcagcacg gtgaacacct
      721 gttccggcgt ttcaacgtgg tagcgcccga aatgccgtgc cgcctcgacg ttggtgatca
      781 cgcccacctg acagcgatcg taggccaggc cttcgctgag gatgctgtcg gttccgttct
      841 cgaaaactgc ggcttcgacg gcgcgattca tcagcagtcg gtgggcgcct ccccagttgg
      901 cgttgtcgcc ctgtgcgata caacgccgat gcacgaacaa tccgtcgctg ctggccagcc
      961 ccgtgtgcct gccggacagg gtcaggagac tggcgatcag acgggcgacg gtcgtcttgc
     1021 cgaagctgcc ggtcacgccc accaccggga tacgtccgtc gtcgccgttg gggaagagtt
     1081 catcgacgat ggcgcgaccg accgggcgtg gctggccggt ctcgggcttg aggtgcatca
     1141 gcaggccagg accggcgtta acctcgacaa tgccgccgcg ttgcccgtcg agaggctgcg
     1201 aaatgtcttc gcagaccaga tccaccccgg cgatgtcgag tccgacgata cgtgcggcga
     1261 gcgaggcggt ggctgcggtt tccgggtgta ccagatccgt gacgtcgtcc gtatgattgc
     1321 cgctgcggac gatcaggacc tcacgaccgg ccgccgggac cgagtccggg gcaaatccct
     1381 ggcgcgagac ttcgagcctg gcaaccgggt tgtcggcgag ggcaatgata tcgagcggga
     1441 actcctcggc ggcgccgcgt cgcgggtcgg agttgatctg gctatcgatc agttcgtcga
     1501 tcgttgactg gccatcgccg acgaccttgg cggtttcgcc acgcgcggcc gcagcgagtt
     1561 tgccgccaac gatcagcagt cgatgttccg agccgcgcac gtaacgttcg acgatgacgc
     1621 cgctgccttc gacgaccgcc gccgcatagg ccgactcgac ctcttcgcgg gccatcagat
     1681 tggtgaacac gccgcgcgcg tggttgccgt ccgatggttt gacgaccacc ggcaagccga
     1741 tctcttcggc cgcatcccag gcatcttcgg cactatcgac caggcgtccc tcgggaaccg
     1801 gtacgccaca ggatttgagg agcgtcttgg tgagatcctt gtcgcgcgag atcccttcgg
     1861 caatcgcgct ggtacggtcg gtttcggcag tccagatgcg ccggctgcgg gcgccgtagc
     1921 cgagttgcac gaggttgccg gtggccagca gacgaatcgc cggaatgcga cggtctttgg
     1981 cggttgcggc ttcgacgatg cagcccgtgc ttggaccaag gagcttgcgt tcggccatgt
     2041 cggccagtcg ttcgactgtg ccggcaacgt cgaaggtctg gtccttgatg gcggccagca
     2101 gcaggtcgcg tcccgcatag agacaggagc gtgtgacgtc ctcatgccag gcgcgcacca
     2161 cgacccggta tacgccgcgc gtcgacgatt cacgcgcctt gccgaacccg ccgggcatac
     2221 cggcgagatt ctgtagttcg agggtgacgt gttcgagcat gtgcgctgcc caggtgcctt
     2281 cctcgacgcg ccgcaggaag ccgccgcgtt ctccgtaact gcagcggtgc tcgatcagcg
     2341 aaggcaacag ggcgatgagc cgttcgtgga aacccgggat ggtgttggag ggaaagtcct
     2401 cgagttcgcc gatatcgacc agcgcttcga tgaccggacg gtaagtccag atgtttgggc
     2461 cgcgaaggta gcggatctcg aggaacttga tgtctttgct tttcatggcg tcagtccagg
     2521 cacggggagg ttggcgcgcg acggcgcggg acaggcgtaa ggccatcgga gtaccggccc
     2581 ggcccggatt atctcgggaa taacgccggc tgcgtcaatc ggctgaccgc cggtcaagcg
     2641 gattgctccg ggtgtcgtca caggaagcgg tccaggattc gtcggctgtg ctgatcgagg
     2701 gcatagcggt cgcggatcag gaagtggatg ccgtggctgt cagcgatcaa cagcgccggc
     2761 gaggtgaggc ggcgaatgtc ttcctcgccc ttcaggacga aactcgcggc accgcggtcg
     2821 gtctcgacct gccaggtgct cggtgtggcg aaactcgcca caccgacgat gcgcgtgatc
     2881 tcgggcatga actcgcgctg ggcgagttcg gactcaacga ggatgcgcag atggtccggc
     2941 agtgcgtcga gccggtcgat ccacgccagt tcgtggccgt aggggtctac caggccgatg
     3001 ccgtcgtcgg ggtcggtgat cggaaaagcc cgcacgggta ccacaacggc atggacttcg
     3061 ccgtcggcac caatgaattc cagcttgccg aaggcgttac ggcgtaattg gtaggcaggg
     3121 tcgatggtca ttgtcggtct tcctcgtcgt gtcgggcgag ttccatccga cgcagctcgt
     3181 cttcggtgtc gacgttgcgg gcctgcgcct gatacaggcg gtagtaatga ccctcgcaag
     3241 ccatcagttc gtcgtggctg ccgacctcga cgacactgcc acggtcgagg acgaccagtc
     3301 gattggcgtc acgcagcgtg gacaggcggt gcgcaatggc aatcgtcgta cgtcctcgta
     3361 ccaggttgtc gagtgccttc tggatttcct tctcggtggt ggtgtcgacc gacgaggtcg
     3421 cctcgtcgag gatcaggatg cgagggtcga tcagcagcgc gcgggcgatc gagatccgct
     3481 gtcgttcccc gcctgaaagt gcctgcccgc gttctccgac cagtgagtcg tagccgtgcg
     3541 gcaggcgaag gatgaattcg tgggcgtgcg cggcgcgcgc cgcggcgacg atctccgcat
     3601 gcgtcgcatc gggtttgcca taggcgatat tctcggcaat ggttccgaag aacaggaagg
     3661 gctcctgcag taccagcccg atgtgcttgc ggtattcggc gaccggcaac gaccggatat
     3721 cgacaccatc gatcaggatg gcgccttcgg tgacgtcgta aaagcggcag atcaggttga
     3781 ccagggtgct ctttccggaa ccgctgtgtc cgaccaggcc gaccatttca ccgggcctga
     3841 tgacgatgtt gatgtcgcgg gtgacgctgc gcgtgccata gcggaagccg acgccgcgca
     3901 actcgatgcg tcccgaaacc ttcggcagat gtaccgggtg cgtcggttcg ggaacgctcg
     3961 agacgtggtc gagaatgtcg aagatgcgct tggcgcctgc cgcagccttc tgcgtgaccg
     4021 agacgatgcg gctcatcgag tccaggcgca ggtagaagcg gctgatgtag gcgaggaacg
     4081 cggtcaatac gccaacggtg atctgatcct tggcgatctg ccagatcccg aaggcccaga
     4141 cgatcaacaa accgatttcg gtgagcagcg tcacggtcgg cgagaacacc gacca
//
LOCUS       DAOSIT010000162         4186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18732_length_4186_cov_9.589446, whole genome shotgun sequence.
ACCESSION   DAOSIT010000162 DAOSIT010000000
VERSION     DAOSIT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18732_length_4186_cov_9.589446"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLL14_05200"
     CDS             <1..461
                     /locus_tag="PLL14_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOG03352.1"
                     /translation="LPFVFDPGQGLPMFSGDDLMDFMRLATYACFNDYEAKLLCDRTG
                     QTLEQLAAEVEALVVTLGGAGSHLYAQGRCHAIPCVEADAVVDPTGCGDAFRSGLLYG
                     IVAGWDWEKTGRLAAVMGAIKIAHRGGQNHRPSRAEIADLFARAFGTAPW"
     gene            complement(540..1172)
                     /locus_tag="PLL14_05205"
     CDS             complement(540..1172)
                     /locus_tag="PLL14_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03353.1"
                     /translation="MQPDIVGTITDTALVADGSMNALYSPHNIEGLCPARPVAADYGS
                     PLKAAIFFWPWQCQSCYFQPSTDLRTRRVPLNRLFAMTFLPAVLAACATTEPQSAAAS
                     ASHHRSGKDKEQKQLNFKLASGVYRCELGQSVEVQRSSQNVNRIELGWLGNRHTLLRQ
                     DSSSGLPRYEDRQKGLLWIDLPWKSVLMDVNSGRPLANECKLAPGQTTRG"
     gene            1890..2267
                     /locus_tag="PLL14_05210"
     CDS             1890..2267
                     /locus_tag="PLL14_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03354.1"
                     /translation="MNIRKRLLVPERRRELPAHFSWLDHRLVQEHHIERAEVSAWALY
                     LFLVTVGDADGLSYYSDAALARRLGLDLSRLVRARKALLGLDLIAFEPPLVQVLSLET
                     PVPIADRFAHLRAVLQGSTGAQP"
     gene            2264..3739
                     /gene="istA"
                     /locus_tag="PLL14_05215"
     CDS             2264..3739
                     /gene="istA"
                     /locus_tag="PLL14_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG03355.1"
                     /translation="MIDYATWCAIRDGAARHLTPAQLAEELGLDIKTVRRWIDRPYAP
                     RQTARRASQLDPFKGRIVRWLEAHPLSAQQVFQRLGEEGFAGGISIVKDYVRLVRPRP
                     REAFLTLSFAPGECAQADWGEFGTLAVGSAQRRLSFFVMVLAYSRQLYVEFSLAQTME
                     FFLGAQINAFNAFGGVPKKVMIDNLRCGVVQHRRGEPPVFNPRYLDFARHYGFEIVAC
                     NVCRGNEKGRAERGVGYVKHNFLQGRELPEFAALNSAAALWLETVANVRVHGETHRRP
                     VDLWTEERAFLQAVNPRPFDVGRVMTARADRQFRVTFEGNRYSVPARFAGCPVVLKAY
                     PDRLCVYREEQLIARHARCFDRHQEQADPDHAKPLLAQRRHARAQHALTRFLALSAEA
                     RAYHAGLLERRAHADAHVQKILALLEIHGEAPVLRAIADALAFQAFSSEYIAHLVEAR
                     QRQLPEASPLHLTRRQDLLDLELPPPDLSRYPVPPLDGSPS"
     gene            3736..>4186
                     /locus_tag="PLL14_05220"
     CDS             3736..>4186
                     /locus_tag="PLL14_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03356.1"
                     /translation="MKSEILLEQGLARLQLPFFAKHHAAISSEAAQAAWSHARFLETL
                     VVGEVARRDEALIQRRVKAARLPGIKTLDQFDWSFPHKINRAQIQHLFRLGFLKEHTN
                     VILLGGVGVGKTHLATALAHTACLHGQTTLFTSAVDIVNSLAAVQLAG"
BASE COUNT          699 a         1293 c         1355 g          839 t
ORIGIN      
        1 gccttccctt cgtgttcgat cccggccagg gtttgccgat gttttcgggc gacgatctga
       61 tggacttcat gcgtctggcc acctatgcct gcttcaacga ttacgaggcg aagctactgt
      121 gtgaccgcac tggccaaacg ctcgagcaac tcgccgccga ggtcgaagcg ctggtcgtta
      181 ccctcggggg tgcaggctcg cacctctatg cgcaggggcg atgccatgcg attccctgtg
      241 tcgaggccga cgcagtcgtc gacccaacgg gatgtggtga tgctttccgc tcgggtctac
      301 tctatggaat tgtcgccggc tgggattggg agaagaccgg ccgtctcgcg gcggtgatgg
      361 gggcgatcaa gattgcccac cgaggaggtc agaaccatcg cccgagccgc gctgaaatcg
      421 ccgacctctt cgccagggcg tttggcacag ccccctggta aggcgacgtg atggccatcg
      481 taaagccgcc gctggcgatg cgcagcgcgg ctgcactcgc cgagcacacc gggggaggtt
      541 cagccgcgcg tggtctggcc tggcgcgagc ttgcactcgt tggcgagagg ccgtccactg
      601 ttgacgtcca tcaggacgct cttccagggc aggtcgatcc acagcaggcc tttctgccgg
      661 tcctcgtagc gcggcaggcc ggagcttgag tcctgccgca gcagggtgtg gcggttgccc
      721 agccagccga gttcaatccg attcacattt tgcgaactgc gttggacttc cacgctttgg
      781 ccgagttcgc aacgatatac gccgctggcg agtttaaagt tcaactgctt ttgttccttg
      841 tccttgccgc tcctgtggtg ggaagcactt gccgctgccg actgcggttc tgtcgtcgca
      901 caggcggcca gcactgccgg caggaaggtc atggcaaaca ggcgattcag cggcacgcgt
      961 cttgtcctca agtccgtgga aggctgaaaa tagcatgact ggcactgcca gggccagaag
     1021 aagattgcgg ccttgagggg tgagccgtag tctgcggcaa ccggccgcgc cgggcagagc
     1081 ccttcgatgt tgtgcggcga atagagcgcg ttcatgctgc cgtcggctac cagagccgtg
     1141 tcggtgatcg tcccgacgat gtcgggttgc actgcgctgt cgatgtcaag cccgcattcc
     1201 tgtcgccgcc gacctgaaag tggtctgaac ctgacgattt gaatctggtg aagaagcgcc
     1261 ctgttgccgt gcatcttctt cctgcttttc ttatcgccgc tgtcgcttct tccgctccct
     1321 ccgtcttctt cggttttctt cgttattgct tggcaacgcc gcaccatagc cgggatgaat
     1381 tcctgtcgcg aggttcggga tgggtcgaga agcggagcgt aagtgcctga attgcggtaa
     1441 gtcgttcaaa ccggacgcgc ggaacgtgcg gcaccagcag tattgctcgg ccaccttgtg
     1501 ccaaggggcg agcaagcggg ccagccaggc gcgttggctg gccaaagccg agaatcgggg
     1561 ctatttcatg ggcgcgcaag ccgtcgcccg ggtacaggtg tggcgtgcgg cacacccggg
     1621 ctacagccgg cgagggccga cgggggagca accgcccccg agctctgaag cgttacaaga
     1681 agtcttgccg gcccaaccct ctgaatcctc gatgatttct tgtaacgccc cggaacccgt
     1741 cacagcaccg ttacaagatg tcttgagccg gcaaccgact gtaatcattg ggcttattgc
     1801 ccatttgtgg gatagcccgt tgcaagagga catcgcggcg atcctccatc gtctgctaca
     1861 actaggcgag gatatcgaag gaggacgcca tgaacattcg caaacgcttg ctggttccgg
     1921 aacgtcggcg ggagttgccg gcacatttca gctggctcga ccaccggctg gtgcaggagc
     1981 atcacatcga gcgggctgag gtcagcgcct gggcgctgta tctcttcctg gtcactgtcg
     2041 gtgatgccga cggcctgagc tactactcgg acgccgcact ggcgcgacgg ctgggcctgg
     2101 atctttcgcg tctggtgcgc gcgcgcaagg cgctgctggg actcgacctg atcgcgtttg
     2161 agccgccgct ggttcaggtc ctgagcctgg aaacgccggt gccgatcgct gaccgcttcg
     2221 cgcatctgcg ggcggtactg cagggatcga cgggagcgca gccatgatcg attacgccac
     2281 ctggtgtgcc atccgcgacg gtgcggcccg tcatctcacc cccgcgcaac tggccgagga
     2341 actgggtctc gatatcaaga ctgtccggcg ctggatcgac cggccgtatg cccctcggca
     2401 aaccgccaga cgtgcaagtc agctcgatcc gttcaagggc cgcatcgtgc gctggctgga
     2461 agcgcacccc ttatccgccc agcaggtctt tcagcgcttg ggcgaggaag ggttcgccgg
     2521 cgggatcagc atcgtcaagg actacgtgcg cctggttcgc ccgcgtccac gcgaagcgtt
     2581 tctgacgctc tccttcgccc ccggcgaatg tgcacaagcc gattggggcg agttcggcac
     2641 cttggcagtg ggttcggccc agcgtcgcct gtcgttcttt gtcatggtcc tcgcctatag
     2701 ccgacagttg tacgtcgagt tcagcctggc gcagacgatg gagttcttcc tcggggcgca
     2761 gatcaatgcc ttcaacgcct ttggcggtgt cccgaagaaa gtgatgatcg acaacctgcg
     2821 ctgcggggtt gtgcagcacc ggcgcggcga gccgccggtg ttcaacccgc gctatctgga
     2881 tttcgcccgc cactacggct tcgagatcgt tgcctgcaat gtttgccgag gcaatgagaa
     2941 ggggcgtgcc gaacgcggcg tggggtacgt caagcataat ttcctgcaag gccgcgaact
     3001 gcccgagttc gcagcgctca actcggcggc ggcgctgtgg ctggagacgg tggcgaatgt
     3061 gcgcgtgcat ggggagacgc atcgccgtcc ggtcgatctg tggaccgagg aacgcgcctt
     3121 cctgcaggcg gtgaatccgc gtccctttga tgtcggaagg gtgatgacgg cgcgcgccga
     3181 tcgccagttc cgggtgacct tcgagggcaa tcgttattcg gtgccggcgc gctttgccgg
     3241 ttgtcccgtg gtgctcaagg cgtatccgga tcgactctgc gtttatcgcg aggagcagtt
     3301 gatcgcacgg catgcccgct gcttcgatcg ccatcaggag caggcggacc cggatcatgc
     3361 caagcccctg cttgcgcagc gccgacatgc gcgggcgcaa cacgcgctga cgcgctttct
     3421 ggcgctatcc gccgaagcca gggcgtatca cgccggtttg ctcgaacggc gggctcatgc
     3481 cgacgcgcac gtgcagaaga ttctggcgct gctcgagatc cacggggaag caccggtgct
     3541 gcgggcaatc gccgatgcgc tggctttcca ggcgtttagc agcgagtaca tcgcgcacct
     3601 ggtcgaggct cgccagcgcc agttgccgga agcgagccca ctgcacttga cacgccggca
     3661 ggacttgctg gaccttgaat tgccaccgcc cgacttgtcg cgctatccgg tcccgccgct
     3721 cgatggaagt ccctcatgaa atccgagatc ctgctggagc agggcctggc gcgcctgcag
     3781 ttgccgttct tcgccaaaca tcatgccgcg atcagtagcg aggcggcgca ggcagcctgg
     3841 agccatgccc gctttctgga aacgttggtc gtcggggaag tcgctcgtcg cgatgaggcg
     3901 ctgatccagc gccgggtgaa ggcggctcgc ttgccgggga tcaagacgct cgatcagttc
     3961 gactggagtt tcccgcacaa gatcaaccgc gcccagattc agcacctctt tcgcttgggc
     4021 ttcctgaagg aacacaccaa tgtgatcctg ctcggcggcg tcggtgtcgg caaaacgcat
     4081 ctggcaaccg ccctggcgca cacggcgtgc ctgcatggcc agacgacgct gtttaccagc
     4141 gcggtcgata tcgtcaattc gctggccgcc gttcaactcg cggggg
//
LOCUS       DAOSIT010000163         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18760_length_4182_cov_7.756239, whole genome shotgun sequence.
ACCESSION   DAOSIT010000163 DAOSIT010000000
VERSION     DAOSIT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18760_length_4182_cov_7.756239"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1082)
                     /gene="malQ"
                     /locus_tag="PLL14_05225"
     CDS             complement(<1..>1082)
                     /gene="malQ"
                     /locus_tag="PLL14_05225"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG03357.1"
                     /translation="YALFMALDIAHGGDGRMWQDWPAGLAHRQPAALCEAQARHAEEI
                     RFWQFCQWRFYQQWAALKAYAHSRSIEIVGDLPIFVAAHSADVWSNPGLFELDEQGHP
                     RVVAGVPPDYFSATGQRWGNPLYRWSAHAAEGYRWWVERMRQTMRLCDIVRIDHFRGF
                     ESFWEIPAESATAVDGQWRPGPGKNVFTAMLRELGDAQGRLRIIAEDLGIITPEVDAL
                     RQSIGLPGMRILQFAFDGDARNPYLPHNYDANTVVYTGTHDNDTSRGWWESLGRPEQE
                     YVRSYLGIGEEAGVEVHWHLIRLACSSVASLCVIPMQDVLGLDSAHRMNEPSRSEGSW
                     EWRFSWQQVEAEHAGRLAALARLYGRR"
     assembly_gap    1083..1182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1183..1376)
                     /locus_tag="PLL14_05230"
     CDS             complement(<1183..1376)
                     /locus_tag="PLL14_05230"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG03358.1"
                     /translation="MTSNLRRGGILLHPTSLPGPHGSGDLGPAAYHFVDWLVAAGQSC
                     WQVLPLGSAGPGNSPYISPSA"
     gene            complement(1456..>2238)
                     /gene="ppk2"
                     /locus_tag="PLL14_05235"
     CDS             complement(1456..>2238)
                     /gene="ppk2"
                     /locus_tag="PLL14_05235"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOG03359.1"
                     /translation="AKRMSERVYLKELEKLEAELVHLQSWVIEKGLKVVILFEGRDGA
                     GKGGTIKAITGRVSPRVFRVVALPAPTEREKTQMYVQRYLPHMPAAGEVVIFDRSWYN
                     RAGVERVMGFASDEQVQGFLKLAPLFEKLMVGENNHIILLKYWLEVDMDEQTRRLEAR
                     ITDPRKQWKLSPMDLKSYNRWYDYSRARDEMFKATDTEWAPWFVARSDDKKRVRLNII
                     KHILSSIPYEVVKQPKITLPKRQKPGDYKEINLPLKYVPEVY"
     assembly_gap    2239..2338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2339..3747)
                     /locus_tag="PLL14_05240"
     CDS             complement(<2339..3747)
                     /locus_tag="PLL14_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOG03360.1"
                     /translation="MRKPLLTTLLALLVGGCMVGPDYVRPPIDTPTAWRLSDQAARDL
                     ANTAWWEQFGDPVLNELVSAALLANKDLLIASARIEEFAGQYGFVRSALFPQVGAGYE
                     VSRQKGVVPGASTSSTSSASSTYNSYQAVLNASWEIDIWGRIRRQTEAARAQLLASED
                     GRRAVILSLVGSVAGAYVNLRDLDRQLEIARATAKSRGDSYEIFKERYAGGIISLLEL
                     SQNQSQYEEALATIPAIEKSIVQQENGLSVLLGRNPGPITRGRNIDQLLLPAIPAGLP
                     SDILERRPDIQQAEQGLIAANALIGAAKAAYFPTLSLTGLFGYASSSLGNLFDSQSKV
                     WQYAAPISMPIFTAGAIAGQVQAAEAQQQQALFAYQRAIQQAFREVNDALIDQDRTRA
                     QLEAQKRQVGALEQYAGTARLRYENGYTSYIEVLDAERSLFNVQLQYTQTQQTQLQAM
                     INLYKAMGGGWVVEAEKVAV"
     gene            complement(3740..>4182)
                     /locus_tag="PLL14_05245"
     CDS             complement(3740..>4182)
                     /locus_tag="PLL14_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG03361.1"
                     /translation="LNVYAQIGLVMLIGLAAKNAILIVEFAKMEYEKGELSLAEATLK
                     GAQLRLRPILMTSFAFILGCVPLAIASGAGAISRQVMGSAVIGGMLAATCIAIFIIPV
                     TFYVVEKISHKGDIGVGHHDGESAAAATESSTVQIAGKEGDSHA"
BASE COUNT          745 a         1241 c         1207 g          789 t
ORIGIN      
        1 cggcgtccgt acagtctggc aagagcggca agccttccgg cgtgttccgc ctcgacctgc
       61 tgccaactga accgccattc ccaggatccc tcgctacggc tcggctcgtt catgcgatga
      121 gccgaatcaa ggccgagcac atcctgcatc gggatgacgc aaagcgatgc caccgacgaa
      181 caggccaggc ggatcaggtg ccagtgcacc tcaactccgg cctcttcgcc aatacccagg
      241 taagagcgca catactcctg ctccgggcgg ccaagcgact cccaccagcc acggctcgtg
      301 tcgttgtcgt gggtgcccgt atagacaacg gtattggcgt cgtaattgtg cggcagatag
      361 gggttcctgg cgtcgccgtc aaaagcgaac tgcaggatgc gcatgccggg caaaccaatc
      421 gactggcgca gagcgtcgac ttcaggggtg atgatgccga gatcttcggc gatgatccgc
      481 agccgcccct gggcgtcgcc cagttcgcga agcatggcgg tgaatacgtt tttccccggc
      541 cctggccgcc actgtccgtc gactgccgtc gcggactcgg cgggaatctc ccagaaggac
      601 tcgaagccac gaaagtgatc gatgcgtaca atgtcgcaga gcctcatcgt ttgccgcatc
      661 cgctcgaccc accagcgata gccttcggcg gcgtgtgccg accagcgata gagcgggttg
      721 ccccagcgct gcccggtggc gctgaagtaa tccgggggga ccccagccac cacgcgcgga
      781 tgaccctgtt cgtcaagttc gaaaagcccg ggattggacc acacatcggc actgtgtgcg
      841 gcaacgaaga tcggcaggtc accgacgatt tcgatgctgc gcgaatgggc ataggccttg
      901 agtgccgccc actgctggta aaaacgccat tggcagaatt gccagaacct gatttcttcg
      961 gcatgccggg cttgtgcttc gcagagtgcc gccggctgac gatgggccaa tccggccggc
     1021 caatcctgcc acattcggcc gtctccacca tgggcaatgt ccagggccat gaacagcgcg
     1081 tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgaagg gctgatgtag
     1201 ggtgaattgc ccggcccggc gctacccagc ggcaacacct gccagcacga ctgaccagcg
     1261 gcaaccagcc agtcgacgaa gtgataagcg gccggtccca gatcgcccga accatgcggg
     1321 ccgggcagcg aggtcgggtg aagcagaatc ccgccacggc ggagatttga agtcatgctg
     1381 atcccttgtc tggagcttga tgtcgtccgc tgccgggata tggacggatc aggtcacagc
     1441 cccggcggtc ggaagtcaat agacttccgg aacgtacttc agcggcagat tgatttcctt
     1501 gtagtcgcct ggcttctgcc gtttcggcaa ggtgatcttt ggctgcttga cgacctcgta
     1561 cgggatgctg ctcagaatat gcttgatgat gttcaggcgt acgcgtttct tgtcgtcgga
     1621 gcgggcgacg aaccagggcg cccactcggt gtccgttgcc ttgaacattt cgtcgcgcgc
     1681 gcgcgagtag tcgtaccagc ggttgtacga cttgaggtcc atcggcgaca gcttccactg
     1741 cttgcgcgga tcggtgatgc gggcctccag gcggcgcgtc tgctcatcca tgtcgacttc
     1801 gagccagtat ttgagcagga tgatatggtt gttttcccca accatcagtt tttcgaagag
     1861 gggcgccagt ttgagaaaac cctgaacctg ctcgtcgctg gcgaagccca tgacgcgttc
     1921 gacaccggcg cggttgtacc aactacggtc gaagatcacc acctctccgg ctgccggcat
     1981 gtgcggcagg taacgctgca catacatctg ggtcttctcg cgctcggtcg gagccggcag
     2041 ggcgacgaca cggaagacac gtgggctgac gcgcccagtg atcgccttga tggttccccc
     2101 cttgccggcc ccgtcacggc cttcgaagag gatcaccact ttcaggccct tctcgatcac
     2161 ccaggattgc aggtgtacca actcggcctc gagtttttcg agttccttga ggtatacccg
     2221 ctcggacatt cttttcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     2341 ggcgactttc tccgcctcga ccacccagcc gccgcccatt gccttgtaga gattgatcat
     2401 cgcctgcaac tgggtctgtt gggtctgcgt gtactgcaac tgaacattga acaagctccg
     2461 ctccgcgtcc aggacctcga tatagctggt gtagccgttt tcgtagcgca gcctcgccgt
     2521 ccccgcatac tgctccagag ctcccacctg gcgtttctgc gcctccaact gggcacgcgt
     2581 gcggtcctgg tcgatcagcg cgtcattgac ttcgcgaaag gcttgctgga tggccctctg
     2641 atacgcgaac agcgcttgct gctgttgcgc ttccgccgcc tgcacctgac cggctatggc
     2701 accggccgta aagatcggca tgctgatcgg cgcagcgtac tgccagacct tcgactggct
     2761 gtcgaagaga ttgccaaggc tgctgctggc gtagccgaac agaccggtca gcgagagggt
     2821 cggaaagtaa gcggccttgg ccgcaccgat cagggcattg gcggcaatca ggccctgctc
     2881 cgcctgctga atgtctggcc gtcgctcaag aatatccgag ggtagaccgg ctggaatggc
     2941 cgggagcaac agttgatcga tgttcctgcc gcgggtaatc ggccctgggt tgcgaccgag
     3001 caacacgctc aggccgttct cctgttgtac gatcgacttc tcgatggccg ggatggtggc
     3061 cagtgcttcc tcgtactggg actggttctg actcagttcg agcaacgaga tgatgccacc
     3121 cgcgtaacgc tccttgaaga tctcgtacga atcgccgcga cttttcgccg tcgccctggc
     3181 gatttccaac tggcgatcga ggtcgcggag gttgacgtag gcaccggcaa cgctaccgac
     3241 cagagagagg atgaccgctc gccggccgtc ctcgctggcc aggagctgtg ccctcgccgc
     3301 ttcggtctgg cggcgaatgc ggccccagat gtcgatctcc caactggcat tgagcacggc
     3361 ctgatagctg ttgtaggtcg atgaggcaga agaggtcgat gaggttgatg cgcccggaac
     3421 gacgcccttt tgtcgactca cctcatagcc ggctccgacc tgcggaaaga gggcggaacg
     3481 gacgaatccg tattggccgg caaactcttc gatgcgcgcg ctggcaatca gcagatcctt
     3541 gttggcaaga agggcggcgc tgaccagttc gttgagtacc gggtcgccga attgttccca
     3601 ccaagcggta ttggccagat ccctggctgc ctggtcactc agacgccagg cggtcggcgt
     3661 atctatcggt ggacgcacgt aatcaggccc aaccatgcag ccaccaacca gcagcgccag
     3721 cagtgtggtc aacaagggtt tacgcatggc tatccccctc ctttccagca atctgtactg
     3781 tcgaactctc ggtggcagcg gcggcgctct cgccatcgtg gtggccaacc ccgatgtcgc
     3841 ccttgtgact gatcttctcg accacgtaga aagtcaccgg aatgatgaag atggcaatgc
     3901 aggtagcggc cagcatgccg ccgatcaccg cgctgcccat gacctgtcgg gaaatggcgc
     3961 cggcgccgct ggcgatggcc aggggcacac agccgaggat gaaggcgaag gaagtcatca
     4021 ggatcggccg cagacgcagt tgtgcgccct tgagcgttgc ctccgccagc gacagctcgc
     4081 ccttctcgta ctccatcttg gcgaattcga cgatcaggat cgcgtttttc gccgccaggc
     4141 cgatcagcat caccagaccg atctgcgcat agacgttgag tt
//
LOCUS       DAOSIT010000164         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18823_length_4170_cov_6.013366, whole genome shotgun sequence.
ACCESSION   DAOSIT010000164 DAOSIT010000000
VERSION     DAOSIT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18823_length_4170_cov_6.013366"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLL14_05250"
     CDS             <1..1293
                     /locus_tag="PLL14_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03362.1"
                     /translation="DGTPALYVVLSDVTTRKQAELELARQRDHHEHLMQERSAALAVA
                     RDLAEAANRAKSTFLSTMSHELRTPMNGVMGMIELAARRTTDSKSREYLAKALGAAQS
                     LLAIINDILDLSRIEADRLRLDCRRFTLGSLLGQLRSLAEPLANAKGIALRVHVPAAL
                     AELAVLGDDLRLGQILLNLTGNAIKFTPAGSVSVRVTVSDEPGEALGLRCEVEDTGIG
                     IAAADQDLLFEPFEQADRLLKRKYGGSGLGLAISRRLAHLMDGEIGLTSEVGVGSVFW
                     CTVRLRKAPVERAPEIPAAGPGAEEERLTIGHRGKRILLVEDDALAREVVRELLEQVG
                     LVVDTAEDGWQAVVLATTNGYHLVLMDLAMPNVDGFEATRLIRALPGCADLPIVALTA
                     YAFDEDRARCLSVGMDDHLAKPVIPDALYGMLCAWLDR"
     gene            complement(1321..2919)
                     /locus_tag="PLL14_05255"
     CDS             complement(1321..2919)
                     /locus_tag="PLL14_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03363.1"
                     /translation="MSVKTRLMAFASLALAALMLVIVVVLLMISRLGTLQDTGFANSQ
                     SAVRAQQASWLGAQFYQIFADAIINRHIEESRNDFFRLRAQAGSDLDRLGQEADTPEE
                     KQAVASARQSVDRLAEIFEKKLLPALSDRNLVDQGIGQIDELADREVREIRVHLDKVA
                     NSMSAEAVAADREFDAASSSTLRKIGIIAIVAALVLLCAALLIIRSIVQPLAGVQAAT
                     RQVAAGDLSRSIEVTGPTELAEVLASCNDMQKGLCQIIAAIQGNAETVAAMSQQLSAT
                     TSQLSTSSGTQADAAASMAASVEQMSVSISLISDQAADLRNSATRSGNAGRAIIGRMI
                     DSGRLTSAAVDQVAGQIEQLGKYSEQISSIIRVIREVAEQTNLLALNAAIEAARAGEQ
                     GRGFAVVADEVRKLAERTGNSTREIGAMIGQVQSVSREAVTGMGEVATQIGGFEQLSR
                     EAGGAIDTIDAHSQQVVTVVEDITRALKEQSIASSDVAARVEQIAQSSEENGATVKET
                     AAAASRLEAVAAQLKGTTQRFRLA"
     gene            3258..4170
                     /locus_tag="PLL14_05260"
                     /pseudo
     CDS             3258..4170
                     /locus_tag="PLL14_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860533.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
BASE COUNT          755 a         1311 c         1307 g          797 t
ORIGIN      
        1 gacggtacgc cggcgctgta tgtcgtgttg agcgatgtga ccaccaggaa acaggccgaa
       61 ctcgaactcg cgcgacagcg cgatcatcac gaacacctga tgcaggaacg tagcgccgca
      121 ctggccgttg ccagggacct cgccgaagcc gccaaccgcg ccaagagcac cttcctgtcg
      181 accatgagcc acgaactgcg cacgccgatg aacggcgtca tgggcatgat cgaactcgcc
      241 gcgcgccgga ccaccgacag caagtcccgt gaatatctgg ccaaggcgct tggtgccgcg
      301 caaagcctgc tggcgatcat caacgacatt ctcgacctgt cgcgcatcga agccgatcgc
      361 ctgcggctcg actgtcgccg ttttacgctc ggctcgctgt tggggcagtt gcggagcctg
      421 gcggagccgc tggccaacgc caagggcatc gccctgcggg tgcacgtccc ggccgcgctg
      481 gccgaattgg cggtcctcgg cgacgacctg cgtctcggcc agattctgct caatctcacc
      541 ggcaacgcca taaagttcac gcccgccggg tcggtgtcgg tgcgcgtcac ggtcagcgac
      601 gagccaggag aagcactagg gctgcgctgt gaagtcgagg ataccggcat tggcattgcg
      661 gcggcggacc aggatctcct gttcgaaccc ttcgagcagg ccgaccgctt gctgaagcga
      721 aagtacggcg gcagcgggct gggcctggcg atcagccgtc gtctggcgca cttgatggac
      781 ggcgagatcg gtttgaccag cgaagtgggc gttggcagcg ttttctggtg tacggttcgc
      841 ttgcgcaaag cgccggtcga acgtgcgcca gagatccctg cggccggtcc cggcgccgaa
      901 gaagagaggc tgacgatcgg ccatcgcggc aaacgcatcc tgctggtcga ggacgatgcc
      961 ttggcgcgcg aggtcgttcg ggaactgctg gaacaagtcg gactcgtcgt cgatacggcc
     1021 gaggacggct ggcaggcggt cgttctggcg acgaccaacg gctaccatct ggtcctgatg
     1081 gatctcgcga tgccgaacgt ggacggtttc gaagcgacgc gactcatccg cgccctgccg
     1141 ggctgcgcgg acctgccgat cgtcgcgctg accgcctacg ccttcgacga agaccgggcg
     1201 cgctgcctgt cggtgggaat ggacgatcac ttggccaaac cggtgatccc cgacgcgctc
     1261 tacgggatgc tgtgcgcctg gctggatcgc taatacccaa tcatggcgcc gccgtcggcg
     1321 ttatgcgagg cgaaagcgtt gtgtcgtgcc cttcaattgt gcggcaaccg cttcgaggcg
     1381 actcgcggcg gcggctgttt ccttgactgt ggcgccgttt tcctcgctgg actgagcgat
     1441 ctgttcgaca cgggcggcaa catccgaact ggcgatcgac tgttccttga gtgccctggt
     1501 gatgtcctcg acaaccgtga ccacttgctg tgagtgtgca tcgatggtgt ctattgcgcc
     1561 tcccgcttcg cgcgacaatt gctcgaagcc accgatctgc gtcgcaacct cgcccatgcc
     1621 ggtgacggct tcgcggctga ctgactggac ctgcccgatc attgcgccga tttcccgggt
     1681 cgagttgccg gtacgttcag cgagtttgcg cacttcgtcg gcaacgaccg caaaacctcg
     1741 cccctgctcg ccggcacgcg cagcctcaat ggccgcgttc agggccagca ggttggtttg
     1801 ttcggccacc tcacgaatca cccggatgat cgatgagatc tgctcggagt acttgccaag
     1861 ctgctcgatt tgcccggcaa cctggtcgac cgcggcgctg gtcaagcgac cgctgtcgat
     1921 catccggcca atgatggcgc ggcctgcgtt tcccgagcgc gttgccgagt tgcgaaggtc
     1981 ggccgcctga tcggaaatca gcgaaatgct gaccgacatc tgttcaacgg aggcggccat
     2041 cgacgctgca gcgtcggcct gggtcccgga ggaagtcgaa agctggctgg tggttgcgga
     2101 caactgctgg ctcatcgcgg cgacggtttc cgcgttgccc tggatggcag caatgatctg
     2161 gcacaagcct ttctgcatgt cgttgcagga cgccagtact tcggccagct cggtggggcc
     2221 ggtgacttcg atcgaccggc tgaggtcgcc agcggcgacc tgccgggtgg ccgcctggac
     2281 gccggccaga ggttgcacga tcgagcggat gatcaacagt gcagcgcaaa gcaggacaag
     2341 tgcggcgaca atggcgatga taccgatctt gcgcagcgtc gacgaactcg ccgcatcgaa
     2401 ctcccggtcg gcggctacgg cttccgccga catcgaattc gccactttgt ccagatgaac
     2461 acgaatctcg cggacttccc ggtcggccag ttcgtcgatc tggccgattc cctggtccac
     2521 cagattccgg tcgctgagcg cggggagcaa cttcttctcg aagatttctg cgagcctgtc
     2581 gaccgactgg cgggcactgg caaccgcctg cttttcttcc ggggtatctg cttcctggcc
     2641 cagacgatcg aggtcactgc ccgcttgtgc gcgcaggcgg aaaaaatcgt tgcgcgactc
     2701 ctcgatatgt cggttgatga tcgcgtcggc gaagatctgg tagaactggg cgcctaacca
     2761 ggaggcttgc tgtgcccgga ctgcgctctg gctattggca aacccggtat cctgaagcgt
     2821 gccgagacga ctgatcatca gcaggaccac gacgatcacc agcatgaggg ccgccaatgc
     2881 gagagaagcg aacgccatga ggcgggtttt gacactcatc accacgtcct gtatctcctt
     2941 atcaatgcga gggaattcga ccgtggcgat tgtctccctg cttctccgca atcgtcaata
     3001 cctggctgac cggggcaatc ccgatttgac agcagcagat gggacgctct cctcccgcag
     3061 ggaaaaccca tcaatcaata cccacgtcta tgatcctata caagctttgt tcacaacccc
     3121 tgtcacgtgt agcttctcgg gtttgcccgg gatgtgtggc gattgccctg ctgcacgcat
     3181 tgagcaaacg agccggtcgt cccttttagg cgtaagatgg tgtatagcga acgcgggcaa
     3241 tcgatcggag accgagcatg aacgccgcaa gaacgaccag gaagacctcg ccgcaatcgg
     3301 tagcgtcgcc tgacgctgcg gaggtgaaag acgacagctt tccgatcgtc ggcatcggtg
     3361 cctcggctgg tggtttgtcg gcattcgagg cctttttctc gggcatgccc gccgacagtg
     3421 accctgggat ggccttcgtg ctggtccagc acctggcgcc ggaccacaag agcattctcg
     3481 ccgacctgat ccgacgctat acccgcatgc aggtcttcga ggtcgaggac ggcatgtcgg
     3541 tgcgggtcaa ctgcgcgtca actgcgcgta catcattccg cccaatcacg acatggcgat
     3601 tctgaacggc acgctgcaat tgctcgaacc gtccgagccg cgcggccgac ggttgccgat
     3661 cgacttcttc tttcgttcac tggcgcagga ccagcatgaa ctggcgatcg gcatcgtcct
     3721 ttcgggaacc ggcagcgacg gcacgctcgg cgtgcgggcg atcaagggcg aggccggcat
     3781 ggtcatggtc cagagcccgg aaaccaccga cttcgacggc atgccgcgca gcgccatcgc
     3841 taccggcctg gtcgattggg agctgccggc ggcggcgatg cccgcgcagt tgatcgccta
     3901 cgtcgcgaac gccttcggca tgtcttccgc gctgaacacc caggcaccga agaacgagaa
     3961 cgcgctgaag aagatcttcg tcctgttgcg cgcgcagacc gggcacgacc tttcccaata
     4021 caagccaagc accatcaacc gtcgcatctt gcgccgcatg gcggtgcacc agatcgaatc
     4081 gattgacgac tacgtccggt atctgcagaa gacctcctac gaggtggacg cgctgtttcg
     4141 cgacctgttg atcggggtga ccagtttctt
//
LOCUS       DAOSIT010000165         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_18884_length_4160_cov_6.721559, whole genome shotgun sequence.
ACCESSION   DAOSIT010000165 DAOSIT010000000
VERSION     DAOSIT010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18884_length_4160_cov_6.721559"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1502)
                     /locus_tag="PLL14_05265"
     CDS             complement(279..1502)
                     /locus_tag="PLL14_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03364.1"
                     /translation="MIADTDRLLEDARLVAEQASRTGQLPDALLPKAIAAVEHARETG
                     GHADYAALLSALNAAIRAIAPITLIDLREGRSPFDAGARRSARRLVALLCVVSIALTA
                     LIGWYTRVLYQAESALTAIQQLASVRYLDKVRAVRRLAQDEEVFTKHDARYAQYHQQL
                     GDLALARAAHNAAVGLIIRASENDSYPFETYFRSLPAHAVAPQQRPAEQAAIAAAPGS
                     GLPAHEPAASAPEDPDPCSTLAVAAASNREASRTHATDLAPKRDLLEDYCFLVSIGDD
                     NPGILFTPTFGAQYTFEIGTQRMILSGWVLPFLYGLLGASVFLMRSLLNERTANMALF
                     PVLLRLALGGIAGLVIGWFSVPGVGTSATQISSIPFGVAFLAGFSIDTLFALLERLTH
                     SLSAAPRRRPANTHG"
     gene            2104..2559
                     /locus_tag="PLL14_05270"
     CDS             2104..2559
                     /locus_tag="PLL14_05270"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG03365.1"
                     /translation="MIDQTVDDFSLPATSDRTFTLSACRGSIVVLYFYPKDSTPGCTT
                     EAQQFRDLYPQFVKAGAVVVGVSRDGIKSHENFKARQNLPFDLLADTEELLCARFAVI
                     KMKSMYGKQVRGIERSTFVIDRQGVLRREWRAVKVAGHAQAVLDFLAQL"
     gene            2586..4013
                     /locus_tag="PLL14_05275"
     CDS             2586..4013
                     /locus_tag="PLL14_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG03366.1"
                     /translation="MPRKPAIGADNDVAAKASKLFVLDTNVLMHDPTSVYRFEEHDVF
                     LPIMTLEELDDHKKGVSEIARNARQVTRTLDEIVTSVDAAIDQGIELFGPSRRLASGR
                     LFLQTEAIVGDLPAGLPTAKVDNQILAVVIHLQRRHPERPVILVSKDINMRIKARALG
                     LQAQDYFNDKVLEDSDLLYTGMRELGADFWDQCGHDVESRKKDGRIRYRLNGPLCAQL
                     LVNQFLYQEGEKPLQAIVREVSATTAEIETLTDHTHPKNNTWGITARNREQNFALNLL
                     MNPEVDFVTLLGQAGTGKTLLTLAAGLTQVLESKRYSEIIMTRVTVPVGEDIGFLPGT
                     EEEKMGPWMGALEDNLEVLNKSDEDAGDWGRAATRDLIHHRIKIKSLNFMRGRTFLNK
                     YLIIDEAQNLTPKQMKTLITRAGPGTKVICLGNIAQIDTPYLTEGSSGLTYVVDRFKG
                     WKHAGHITLQRGERSRLADYAAEVL"
BASE COUNT          877 a         1318 c         1221 g          744 t
ORIGIN      
        1 cttgttgata tcagtcaaca gcgatcgcgg acgggcagtc atcgtggcat gacgctattt
       61 gtcagaaaga agccagaaga gcgcaaattt ttgtagcgct tttcggacca agcacaattc
      121 gcgccagaat tcaacacatg aagttaattg cttgatttta ttgcatgcca gatctgcacc
      181 gccagtttgc cgaacaagca ggcggggatc atcaggagct tgttggctca aggtgtaaaa
      241 tattccgaca gcagcgccgt gcagacgggc gccgcaggtc aaccgtgcgt gttggcaggt
      301 cgccgtcgtg gcgcagcgct aagcgagtgg gtgagtctct ccagcagcgc gaacagcgtg
      361 tcgatgctga atccggccag gaacgcgacg ccaaagggaa tcgaggaaat ttgcgtcgcg
      421 ctggtgccga cacccggcac cgagaaccat ccgatcacca gaccggcgat gccgcccagt
      481 gcgagccgca gcaggacggg aaagagcgcc atgttggcgg tcctttcgtt gagcagcgat
      541 cgcatcagga agacggaggc gcccagcagc ccgtagagga acggcaacac ccagccagaa
      601 aggatcatcc tttgggtgcc gatttcgaaa gtgtactggg ccccgaacgt cggggtgaaa
      661 agtatgcccg gattgtcgtc accaatgctc accaggaaac aatagtcttc cagcagatcc
      721 cgcttcggag ccagatcggt cgcatgcgtc ctgctcgctt cccgattcga agccgccgcc
      781 acggcaagtg tcgaacaagg atcgggatct tccggggccg atgctgccgg ctcatgtgcg
      841 ggaagcccac tgccgggggc tgcggcgatc gctgcctgct cggctggcct ttgctgcggg
      901 gccaccgcat gtgccggcag cgagcgaaaa taagtctcga agggatagga atcattctcg
      961 gatgcgcgga tgatgagtcc cacggccgcg ttatgcgctg cgcgagccag ggccagatca
     1021 ccgagttgtt gatggtactg cgcatagcgc gcgtcatgtt tggtgaacac ctcttcgtcc
     1081 tgcgccagcc ggcgaaccgc gcggaccttg tcgaggtagc ggacactcgc cagttgctgg
     1141 atggccgtca gcgcggactc ggcttgataa agaacgcggg tgtaccagcc gataagcgcg
     1201 gtcagggcga tcgacacgac acaaagcaag gccaccaggc gtcttgcgga tcgtcgcgca
     1261 cccgcgtcaa atgggctccg accctcccgc aggtcgatca gggtaatcgg ggcgatggcg
     1321 cgaatggcgg cgttcaatgc cgacagcagg gccgcgtagt cggcgtggcc accggtctct
     1381 ctggcatgtt ccacggcggc aatggctttc ggcaacagtg catccggaag ctggccggtc
     1441 cgggacgcct gctccgccac caggcgtgca tcttcgagta atcgatccgt gtcggcgatc
     1501 atgtgttgag acccccgatg gcggaccgcc taattgagcg ttccggtacg ggaacccagg
     1561 ggcgtacggc aagtacaggc atctcctgcg cgacccggcg gcgaagccca acaccaggtt
     1621 ttcgcatcga cgacgcagac ggtccccggt cggtgtggtg gtacctgtgc catcaagttt
     1681 ccactgccgg caagcaggct gatgacggcg aacgcgcccg ggaggactct gtgactggcc
     1741 atgacgcagc aatcccatca aacagtggcg gggaacaacc cgttggcgat cgctcccgac
     1801 accagaaaac atccgccccg aatgcgatgc gcctgcacca gggccaggga acggtcgatc
     1861 cagccaaagt ataggtagcc gtatcacgca ctgtcaatgt actctcctcg caggacctcc
     1921 cgatccacct tcaccttgca ctccggattc tctttccgta tgccagcttc tggcttgcac
     1981 cccggtccga attgccggga ctactagggt tgcagaattc ctggccatcg ctcttgtcaa
     2041 tcggcttgac cgatactccg gtttcttaca acaatcaagc tctccgccct ctgaaagccc
     2101 gccatgatcg accagaccgt agatgacttc agcctgccgg caacctctga ccggacgttc
     2161 actctctccg cctgccgcgg gagcatcgtc gtgctttatt tctaccccaa ggacagcacg
     2221 ccgggatgca ccaccgaggc ccagcagttc cgtgacctgt acccgcagtt cgtcaaggcc
     2281 ggcgcagtcg tcgtcggcgt ctcacgtgac ggcatcaagt cgcacgagaa cttcaaggcc
     2341 aggcagaacc tgccgttcga cctgctcgcc gataccgaag agttgctgtg cgcccgcttt
     2401 gcggtgatca agatgaaatc aatgtacggg aaacaggtgc gtggcatcga acgcagcacc
     2461 ttcgtcattg accgccaagg ggtcttgcgg cgcgaatggc gcgccgtgaa agtggccggc
     2521 cacgcacagg cagtgctgga tttcctcgcc cagctgtgac ccaccctcac ccggaaagcg
     2581 ctcgaatgcc ccgcaaaccc gcaatcggcg ccgacaacga cgttgccgcc aaggccagca
     2641 agctcttcgt cctcgacacc aatgtcctga tgcacgatcc gaccagtgtc tatcgcttcg
     2701 aggaacatga tgtcttcctg ccgatcatga ccctggagga actcgacgac cacaagaagg
     2761 gcgtatcgga aatcgcccgc aatgcccgcc aggtgacacg taccctggac gagatcgtca
     2821 ccagcgtgga tgcggccatc gaccagggca tcgaactctt cgggccttcc cgcaggctgg
     2881 ccagcggccg tctgttcctg cagaccgaag ccatcgtcgg cgacctgccg gccggattgc
     2941 cgaccgccaa ggtcgataac cagattctgg cggtcgtcat ccacctgcaa cggcgacatc
     3001 cggagcgtcc ggtaatcctc gtctccaagg acatcaacat gcgcatcaag gcccgcgcac
     3061 tcgggctgca ggcgcaggac tacttcaacg acaaggtcct cgaagacagc gacctcctgt
     3121 acaccggcat gcgcgaactg ggcgccgact tctgggacca atgcggccac gacgtcgaat
     3181 cccggaagaa ggacggccgc atacgctacc gactcaacgg gcccctctgc gcacagttgc
     3241 tggtcaacca gttcctctac caggaggggg aaaaaccctt gcaggccatc gtccgtgagg
     3301 tttccgcgac gacggccgag atcgagacgc tgaccgacca tacgcatccc aagaacaaca
     3361 cctggggaat cacggcgcgc aaccgcgagc agaacttcgc cctcaacctg ctgatgaatc
     3421 cggaagtcga tttcgtcacc ctgctcggcc aggccggtac cggcaagacg ctgctgaccc
     3481 tggcggccgg actgacacag gtgctcgaaa gcaaacgcta cagcgagatc atcatgaccc
     3541 gtgtcaccgt gcccgttggc gaggacatcg gtttcctgcc gggcaccgaa gaggaaaaga
     3601 tgggcccgtg gatgggcgcc ctcgaagaca acctcgaggt actcaacaag agtgacgagg
     3661 acgccggcga ttggggtcgt gcagccaccc gtgatctgat tcaccaccgg atcaagatca
     3721 agtcgctcaa cttcatgcgc ggtcgcacct tcctgaacaa gtatctgatc atcgacgaag
     3781 cgcagaatct gacacccaag cagatgaaga ccctgatcac ccgcgccggc cccggcacca
     3841 aggtcatctg tctcggcaac attgcgcaga tcgatacgcc ctatctgacc gaaggcagct
     3901 ctggactgac ctacgtcgtc gaccgtttca agggatggaa acacgccggg cacatcaccc
     3961 tgcaacgtgg cgagcgctcc cgcctcgccg actacgccgc tgaagtgcta taattttcgg
     4021 gacgttatcg ggccgtgtct tgtgacacgg cccagtcgtt tttggccacc gatccgtgct
     4081 gccggctccg tgcgatatgg atcctacgct taccggagag tcgattgaaa cgacgcgatt
     4141 tccttgctgc ttctgcgctg
//
LOCUS       DAOSIT010000166         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19058_length_4131_cov_8.214671, whole genome shotgun sequence.
ACCESSION   DAOSIT010000166 DAOSIT010000000
VERSION     DAOSIT010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19058_length_4131_cov_8.214671"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLL14_05280"
     CDS             complement(<1..317)
                     /locus_tag="PLL14_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOG03367.1"
                     /translation="MKPQVLFMVVAIASPLAIAAPPPVDPAMLDLADRSRCLSCHDVD
                     ETIRGPAWRDVAKRYRGRPEVEDALVTKVHDGGGGAWGNDYMSANKRAGLDNIRTLVR
                     WILS"
     gene            complement(418..2100)
                     /locus_tag="PLL14_05285"
     CDS             complement(418..2100)
                     /locus_tag="PLL14_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOG03368.1"
                     /translation="MQKGIRGVVMLAALPFAMSVAFAATAEKVATPAPSTGGVTAAPT
                     LSAEDKAAAGKTYFERCAGCHGMLRKGATGKNLEPANTSKLGSSRLEKIMSYGTEGGM
                     PNFDDILTKKEIANMAAYVQMPAEAPPEWSLADIKGSWKLLIPVDKRPTRQMNKVNLA
                     NVFAVTLRDSGEVALIDGDKKTIWAIIKTGYAVHISRMSTSGRYLHVIGRDGRYDLID
                     LWSEVPTTVATLKAGFEARSIETTKFKGYEDKYAVVGSYWPPQYTILDGQTLEPLKNV
                     STRGNTVDGNYHPEPRVASIVASESKPEWVINIKETGMIRLVNYSDLANLKETTINSA
                     KFLHDGGWDSSKRYFLVAANASNKVAVVDTKEGKLAALVDTAKIPHPGRGANFKHPKF
                     GPVWATSHLGADVISIIGTDPSGNPQNAWKVVQELRNHGANSLFVKTHPKSENLWADA
                     PLNPDPKLAGSVTVYRIADLGNPDPKPEVLDLAAAADLGKTKGVQRVVHGEYNEKGDE
                     IWFSVWTGNKTEPSAIVVVDDKARKVKTVIKDPRLVTPTGKFNVYNTMHDIY"
     gene            2321..3106
                     /gene="cobA"
                     /locus_tag="PLL14_05290"
     CDS             2321..3106
                     /gene="cobA"
                     /locus_tag="PLL14_05290"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOG03369.1"
                     /translation="MPQKSTSPLVGTVYIVGSGPGDLDLLTLRAARLIGEADAIVYDH
                     LIAEGVLGLARPEAEKIYAGKESSKHSVPQHELNRLLVSLARAGRNVVRLKGGDPFVF
                     GRGGEEIETLVDSGIPFEVVPGVTSAVGCAAYAGIPLTHREHAQAVAFATGHLKDGTI
                     NLDWPSLARPRLTVVFYMGVGTLAEICRQMIAHGLPPDHPAAVVQNGTTQRQRVLTAD
                     LATLPGKVAAAGVTAPALIIVGTVVRLHRKLTWFSPRLPDEPD"
     gene            complement(3116..3988)
                     /locus_tag="PLL14_05295"
     CDS             complement(3116..3988)
                     /locus_tag="PLL14_05295"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HOG03370.1"
                     /translation="MGAWWFAVRPQTLTVSLVPVILGHALAWSQQGVLHGWLALLALL
                     AALLIQVGTNLHNDVADFERGIDTPERLGPPRATAMGWLPAAAVRRGAWVAFALACNF
                     GVYLAYHGGWPIVLIGLASLLAGWAYTGGPRPIGYTPLGEVFVLLFFGLVAVGGSYYL
                     QTLTISLAALLAGAMVGLFAAAVITVNNTRDLDTDRRAGRRTLAVVLGRRAAGWVYGG
                     ELLLPFVLLPCLALLIGRGVWLVLPLLTLPPVLRLTERFRQAALGPAFNPLLIATARL
                     QFLFGVLLALAVLL"
BASE COUNT          813 a         1322 c         1230 g          766 t
ORIGIN      
        1 gaaagaatcc agcgcaccag ggtgcggatg ttatcgagcc cagcgcgctt gttggccgac
       61 atgtagtcgt taccccaggc gccaccgcca ccatcatgaa ccttggtcac cagtgcatcc
      121 tcgacctccg gcctgccacg ataacgtttg gcgacatcgc gccaggcggg cccgcggatg
      181 gtttcatcga cgtcatggca gctcaggcag cggcttctgt cggcaagatc gagcatcgcc
      241 ggatcgacgg gtggcggtgc ggcgatcgcc agcggcgagg caatggccac gaccatgaac
      301 agaacctgtg gcttcatctt gccatccttg atagagatct acagatgccg accatctacg
      361 accagcgaaa aacggggagc cgaggctccc cgtatggcca cagacgatgc tccgcgatca
      421 gtagatatcg tgcatcgtgt tatagacgtt gaacttgccg gtcggcgtca ccaggcgcgg
      481 atccttgatt accgtcttga ccttgcgagc cttgtcgtcg acgacgacga ttgccgatgg
      541 ctcggtcttg ttgccggtcc acacggaaaa ccagatctcg tcgcctttct cgttgtactc
      601 gccatgcact acgcgctgca cgcccttggt cttgccgaga tcggctgccg cagcgaggtc
      661 aagtacttca ggcttgggat cgggattgcc gaggtccgcg atgcgataaa cggttaccga
      721 accggcaagt ttgggatcgg gattgagcgg cgcatcggcc cacaggtttt cggacttcgg
      781 atgcgtcttc acgaacaagg agttggcacc gtgattcctg agttcctgaa cgaccttcca
      841 ggcgttctgc gggttacctg acggatcggt accgatgatg ctgatcacgt ccgcacccag
      901 gtgtgatgtc gcccatacgg ggccaaattt gggatgtttg aaattggcgc cacgacctgg
      961 atgaggaatc tttgcggtat caaccagagc agccagtttg ccttccttgg tatccaccac
     1021 tgccaccttg ttggaagcgt tggcagcgac cagaaagtag cgcttgctcg aatcccagcc
     1081 gccgtcatgc aggaacttgg cggagttgat cgtcgtttcc ttcaggttgg caagatccga
     1141 gtagttgacc aggcggatca tgcccgtttc cttgatattg atcacccact cgggcttgct
     1201 ctcggaagcc acgatcgagg ccacacgcgg ttctggatgg tagttgccgt caaccgtatt
     1261 gccgcgcgtc gaaacgttct tcagcggttc gagggtctga ccatcaagaa tcgtgtactg
     1321 cggcggccag taggaaccga ccacggcata cttgtcttca taacccttga acttcgtggt
     1381 ttcgatcgaa cgtgcctcaa aaccggcttt cagtgtggca accgtcgtcg gtacttcgct
     1441 ccagaggtcg atgaggtcat agcggccgtc acgaccaatg acgtgcaggt agcgcccgga
     1501 agtcgacatg cgcgagatat ggacggcgta gccggtcttg atgatcgccc agatggtctt
     1561 cttgtcgccg tcgatcaacg ccacctcgcc ggaatcgcgc agcgtgaccg cgaagacgtt
     1621 ggccaggttg accttgttca tctgcctggt tggacgcttg tccaccggga tcagcagctt
     1681 ccagcttccc ttgatgtccg ccaggctcca ctcgggaggg gcttcggccg gcatctggac
     1741 ataggccgcc atgttggcga tctctttctt ggtcagaatg tcgtcgaaat tgggcatgcc
     1801 gccttcggtg ccgtaggaca tgatcttttc gagacgcgaa ctgccgagct tgctggtatt
     1861 ggccggttcg aggttcttgc cggtggcgcc cttgcgcaac atgccgtgac atccggcgca
     1921 acgttcgaaa taggtcttgc ccgctgccgc cttgtcctcg gccgacaagg tcggcgccgc
     1981 cgtcactccg ccagttgacg gggctggtgt cgcgaccttc tcggcagtcg ccgcaaaagc
     2041 cacactcatc gcgaacggca gtgccgccag catcactacc ccacgaattc ccttctgcat
     2101 gccttgctcc ctctcttctt gaaattgctc gactattgcc cgcgcgcgga ccccttccag
     2161 acatcgctca ggatggcatg cctgtacggc tcgattcctt gaccttcgtc aaatattgcg
     2221 aaccgacgcg acaatgcccg aatgagtctc cacgcgggtt gaaatgtgac tgccggggcg
     2281 cttatgcttg cacctcagat ccagagatgg gaaaacaggc atgcctcaaa agtccacttc
     2341 accactagtc ggaaccgtct acatcgtcgg cagcggcccg ggtgacctcg acctgctgac
     2401 actacgcgcc gcccgtctta ttggcgaggc cgatgccatt gtctacgacc atctcattgc
     2461 cgaaggcgtg ctcggactgg ctcggccgga agccgaaaag atctacgccg gcaaggaaag
     2521 ctcgaagcac agcgtgccgc agcacgaact caaccggtta cttgtgagcc ttgcgcgcgc
     2581 gggccggaat gtcgttcgcc tcaagggcgg cgaccccttc gtcttcggac gcggtggcga
     2641 ggagatcgaa accctggtcg actcgggaat tcccttcgag gtggttccgg gagtcacgtc
     2701 cgccgtcggc tgcgccgcct acgccggcat tccgctgaca catcgcgagc acgcacaggc
     2761 tgtcgccttc gctaccggac acctcaagga cggaacaatc aaccttgact ggccttcgct
     2821 ggcacggccg cgattgaccg tcgtgttcta catgggcgtc ggcacccttg ccgagatctg
     2881 ccgccagatg atcgcgcacg gtctgccacc cgatcacccc gcggcggtgg tgcaaaatgg
     2941 cacaacccag cggcagcggg tgttgaccgc cgatctcgcg accctgcccg ggaaagtcgc
     3001 agccgccgga gtcacagcgc cggcactgat catcgttggt acagtagtgc gcctgcaccg
     3061 gaagctcacc tggtttagcc cgcggctgcc cgacgaaccc gactgaattc cgtttctaca
     3121 ggagcacagc cagcgccagc aggacgccaa aaaggaactg caggcgagcg gtagcgatca
     3181 gcaacgggtt gaaggctggc cccagcgctg cctgtcggaa acgttcggtc aaccgcagta
     3241 cgggcggcag tgtcagcagc ggcaagacca gccagacacc tcgcccgatg agcagcgcca
     3301 ggcagggcaa cagcacaaac gggagcagca gttcgccgcc atagacccac cctgcagcgc
     3361 gccggccaag cacgacggcc agagtcctcc tgccggcccg gcgatcggta tcgagatcac
     3421 gggtgttgtt caccgtgatc accgcagccg cgaacagccc gaccatcgct ccggcgagga
     3481 gcgcagcaag gctgatcgtc agggtctgca gataatagct gccaccgacc gccaccaggc
     3541 cgaagaacaa cagcacgaac acctccccga gcggcgtata gccgatcggc cgcggcccgc
     3601 cggtgtaggc ccagccggca agcagcgaag ccagaccaat caggacaatc ggccagccac
     3661 cgtgataggc aaggtagacc ccgaagttgc aggccaaagc aaaggcgacc cacgcccccc
     3721 gccgtacggc ggcggccggc agccaaccca tcgccgtggc acgcggcggt cccagacgct
     3781 ccggcgtatc gatcccgcgc tcgaagtcgg ccacatcgtt gtgcagattg gtcccgacct
     3841 ggatcaacag cgccgccagc agcgcgagga gcgccagcca cccgtgcagg acgccttgct
     3901 gcgaccacgc caaggcatgc cccagaatca ccgggaccag cgagactgtc agggtctgcg
     3961 ggcgcaccgc gaaccaccag gcgcccatcg gcgagggcag cacggcattc aaggcgaaat
     4021 agcgtgcagc acggcgaacc ggcaactgct cagctggccg aaccaaggct tgcgcaggcg
     4081 gtgtgatata atccggcgct ttgcagacac gtgccgtcac tgtcatggtg c
//
LOCUS       DAOSIT010000167         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19091_length_4125_cov_19.646192, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000167 DAOSIT010000000
VERSION     DAOSIT010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19091_length_4125_cov_19.646192"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLL14_05300"
     CDS             complement(<1..269)
                     /locus_tag="PLL14_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753228.1"
                     /note="type IV secretion system VirB9/CagX family; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HOG03371.1"
                     /translation="MKKPLFALLPGVASAIVLAAPGDHLTDLYFSDKNPTLTPQEKAA
                     IAIAEKWKNPMAKGIAPVTGPNGSIKFLYGAQQPSIVCAVLQVCDV"
     gene            complement(283..1056)
                     /locus_tag="PLL14_05305"
     CDS             complement(283..1056)
                     /locus_tag="PLL14_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HOG03372.1"
                     /translation="MNFADPLKRLIGRKPAADADPPRANEPTTWGFRQDESENPYLAA
                     RRTWNEHVGGVVSSRQTWQVIGILSLLIALAGVGGIIHIGSQSKFVPYVVQVDRLGQA
                     VAVAPADRAAPADARVIEASVASFIADARLVTPDVALQRKAVFRVYSMLAPNDPATAR
                     MNEWLNGSEESSPFKRAAKETVSIEIASVLPQTPETWQVDWIETSRDRQGILKGQPFR
                     MRALVTVYVAEPTPSTSEEQIRNNPIGLYVRDFSWAKQL"
     gene            complement(1053..3611)
                     /locus_tag="PLL14_05310"
     CDS             complement(1053..3611)
                     /locus_tag="PLL14_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HOG03373.1"
                     /translation="MIAGIAFAIATLGALLLLVLYARIRAVDEELKLKKHRSRDAGLA
                     DLLNYAALVDDGVMVGKNGAFMAAWLYKGADNASSTDEQREAVSLRINQALSGLGNGW
                     MVHVDAARLPAPNYLDRGTSHFPDGVSAAIDEERRQLFEGLGTLYEGYYVLTLTWFPA
                     LLAQRKFVELMFDDDAKAPDHKAHTHGLIEFFKRECVSIESRLSSAVELQRLRGHRVV
                     NEDGSEVTHDDFLRWLQFCVTGMNHPVQLPSNPMYLDALIGGQELWTGVVPKVGRKFI
                     QVVAIEGFPLESTPGMLTALGELACEYRWSSRFIFMDTHEAVRHLDKFRKKWRQKIRG
                     FFDQVFNTHLGPIDQDALSMVADAEAAIAEVNSGLVAQGYYTSVVVLMDEDRSRLEAS
                     ARQVEKAVNRLGFAARIETLNTMDAYLGSLPGHGVENVRRPLINTMNLADLLPTSTIW
                     TGSNAAPCPMYPPLAPALMHCVTHGATPFRLNLHVRDLGHTFMFGPTGAGKSTHLGIL
                     AAQLRRYAGMSIFAFDKGMAMYPLAAAIRAQTQGSSGLHFTVAADDDQLAFCPLQFLE
                     TKGDRAWAMEWIDTLLALNDLHTTPAQRNEIGHAILSLHASGGRTLSEFSVTIQDEAI
                     RDAIRQYTVDGAMGHLLDAEEDGLSFSDFTVFEIDELMNLGEKYALPVLLYLFRRIER
                     ALHGQPAVIMLDEAWLMLGHPAFRAKIREWLKTLRKANCLVLMATQSLSDAANSGILD
                     VIVESTATKIFLPNLYARDEDTAALYRRMGLNARQIEILATAIPKRQYYYVSENGRRL
                     YDLALGPLALAFVGASDKESLATIQHLESKFGDGWVHEWLAGRGLTLDTYGVAA"
     gene            complement(3608..3919)
                     /locus_tag="PLL14_05315"
     CDS             complement(3608..3919)
                     /locus_tag="PLL14_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HOG03374.1"
                     /translation="MALRKIPIRRAGNRDNLFMGGDRELVMFSGLLAFALVFSAQELR
                     ATVVGLLLWFGALYALRIMAKADPRMRHVYLRHRRYKPYYPARSTPFRDNPASQGKQY
                     Q"
     gene            complement(3922..>4125)
                     /locus_tag="PLL14_05320"
     CDS             complement(3922..>4125)
                     /locus_tag="PLL14_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HOG03375.1"
                     /translation="GGVLIFGGDLNGFFRTLIFVVLVMALLVGAQNMMSSFFGRGAEI
                     VALGAGEFEPVKLSGGIAPGRLA"
BASE COUNT          701 a         1281 c         1411 g          732 t
ORIGIN      
        1 acgtcgcaca cctgcagcac cgcgcagacg atgctcggtt gctgcgcgcc gtacaggaac
       61 ttgatcgagc cgttgggccc ggtcaccggg gcaatgccct tggccatcgg gttcttccac
      121 ttctcggcga tggcaatggc cgccttctcc tgcggcgtca gggtcgggtt cttgtccgag
      181 aaatacagat cggtcaggtg gtcgcccggg gctgccagga caatggcgga agcgacgcca
      241 gggagcaggg cgaagagcgg ttttttcatg agggctcctt tcttagagct gcttcgccca
      301 ggagaagtca cggacgtaca ggccgatcgg attgttgcga atctgttctt cgctggtgct
      361 gggggtcggt tcggcgacat agaccgtcac cagggcgcgc atgcggaagg gctggccctt
      421 caggatcccc tggcggtcgc ggctggtctc gatccagtcg acttgccagg tttccggcgt
      481 ttgcggcagc accgaggcga tctcgatgct gaccgtctcc ttggcggccc gcttgaacgg
      541 gctggactct tcggagccgt tcagccattc gttcatcctg gccgtggccg ggtcgttcgg
      601 cgccagcatc gagtacacgc ggaacaccgc cttgcgctgc agggcgacgt cgggtgtcac
      661 caggcgggcg tccgcaatga aggaggccac cgacgcttcg atcacccgag catcggccgg
      721 tgcggcgcga tcggccggcg ccacggccac ggcttgcccg agcctgtcga cctggacgac
      781 atacggcacg aacttcgatt ggctgccgat gtggatgatg ccgccgacgc ccgccagcgc
      841 gatcaacagc gacaggatgc cgatcacctg ccaggtctgg cgcgacgaca cgacgccgcc
      901 gacatgttcg ttccaggtcc gacgagcggc caggtagggg ttctcgcttt cgtcctggcg
      961 gaaaccccag gtcgtcggtt cattggcgcg tgggggatcg gcgtccgctg cgggcttcct
     1021 gccgatcagg cgcttgagtg ggtctgcgaa gttcatgcgg cgactccata ggtgtccagc
     1081 gtcaatcccc gcccggccag ccactcgtgc acccagccat cgccaaactt gctttcgaga
     1141 tgctggatgg tggccagcga ttccttgtcg gacgcgccga cgaaggcgag cgccaggggg
     1201 ccgagggcga ggtcgtacag gcggcggccg ttctccgaga cgtagtagta ctgacgcttg
     1261 ggaatagccg tcgccaggat ctcgatctgc cgggcgttga ggcccatgcg gcggtagagc
     1321 gccgcggtgt cctcgtcgcg ggcatagagg ttgggcagga agatcttggt cgcggtcgat
     1381 tcgacgatca catccagaat gcctgaattg gcggcgtcgg agagactttg cgtggccatc
     1441 agcaccaggc agttggcctt gcgcaaggtc ttgagccact ccctgatctt ggcgcgaaag
     1501 gccgggtgcc cgagcatcaa ccaggcctcg tccagcatga tcacggccgg ctggccgtgc
     1561 agcgcgcgct cgatacgccg gaagagatag agcagcaccg gcagcgcgta cttctcgccg
     1621 aggttcatca actcgtcgat ctcgaagacg gtgaagtcgg agaacgacaa accatcctct
     1681 tcggcgtcca gcaggtgccc catcgcgcca tcgacggtgt attgacggat cgcgtcgcgg
     1741 atcgcttcgt cctggatggt gaccgagaat tccgacaggg tgcggccgcc gctggcgtgc
     1801 agactgagga tcgcatggcc gatctcgttg cgctgcgccg gcgtggtgtg caggtcgttc
     1861 aaggcgagga gggtgtcgat ccactccatc gcccaggcgc ggtcgccctt ggtttcgagg
     1921 aactgcagcg ggcagaaggc cagctgatcg tcgtcggccg ccaccgtgaa gtgcagacca
     1981 ctgctgccct gggtctgggc gcggatggcg gcggcgagcg ggtacatcgc catgcccttg
     2041 tcgaaggcga agatcgacat gccggcgtag cggcgaagct gcgcggcgag gatgccgagg
     2101 tgcgtcgatt tgccggcgcc ggttgggccg aacatgaagg tatggccgag gtcgcgcacg
     2161 tgcaggttca ggcggaacgg tgtcgcgccg tgggtgacgc agtgcatcag cgccggcgcg
     2221 agtggcgggt acatcgggca gggggcagcg ttgctgccgg tccagatggt gctggtcggc
     2281 agcagatcgg ccaggttcat ggtgttgatc agcggccggc ggacgttttc cacgccgtgg
     2341 ccgggcaggc tgccgaggta ggcgtccatg gtgttgaggg tctcgatgcg ggcggcgaag
     2401 ccgaggcggt tgacggcctt ttccacctgg cgggcagacg cttccaggcg gctgcgatcc
     2461 tcgtccatga gcacgacgac gctggtgtaa tagccctgcg cgaccaggcc gctattgact
     2521 tcggcaatcg ccgcttcggc atcggcgacc atcgacagcg cgtcctgatc gatcggcccg
     2581 aggtgggtgt tgaagacctg gtcgaagaag ccgcgaatct tctgccgcca tttcttgcgg
     2641 aacttgtcga ggtgtcttac cgcctcgtgc gtatccatga agatgaaacg cgacgaccag
     2701 cgatattcgc aggccagctc gccgagggcg gtcaacatgc ccggcgtcga ttccagcgga
     2761 aagccttcga tggcgacgac ctggatgaac ttgcggccga ccttcggcac gacgccggtc
     2821 cacagttcct gcccaccgat gagcgcgtcc aggtacatcg ggttgctcgg caactgcacc
     2881 ggatgattca tgccggtgac gcagaactgc agccagcgca ggaagtcatc gtgggtgact
     2941 tccgatccgt cctcgttgac cacccggtgg ccacgtagcc gctgcagctc caccgccgac
     3001 gacaggcgcg actcgatgct gacgcactcg cgcttgaaga actcgatcag gccgtgggtg
     3061 tgcgccttgt ggtcgggtgc cttggcgtca tcgtcgaaca tcagctcgac gaacttgcgc
     3121 tgcgcgagca gcgccgggaa ccaggtcagc gtcagcacgt agtagccttc gtacagggtg
     3181 cccaggccct cgaacagttg ccgccgctcc tcgtcgatcg ccgccgacac cccgtcggga
     3241 aaatgggagg tgccacgatc gagatagttg ggtgctggca gccgtgctgc atcgacgtgc
     3301 accatccagc cattgccgag gccggagagc gcctggttga tgcgcaacga aacggcctcg
     3361 cgttgctcgt cggtgctgct ggcgttgtcg gcgcccttgt acagccaggc ggccatgaag
     3421 gcgccgttct tgccgaccat cacgccatca tcgaccagcg cggcgtagtt gagcagatcg
     3481 gccagcccgg catcccggga gcggtgcttt ttgagcttca gttcctcgtc gaccgcacgg
     3541 atgcgggcgt agagcacgag cagcagcagg gccccgaggg tggcaatggc gaaggcaatt
     3601 ccggcgatca ttggtactgc ttcccctggc tggccgggtt gtcccggaac ggcgtgctgc
     3661 gcgccgggta atacggcttg tagcgacggt gccgcaggta cacgtggcgc atccgggggt
     3721 ctgccttggc catgatccgc agcgcgtaga gcgcgccgaa ccagagcagc agcccgacga
     3781 ccgtggcccg cagctcctgc gcgctgaaga ccagcgcgaa ggccagcaga cccgagaaca
     3841 tgaccagttc acgatcgccg cccatgaaca ggttgtcccg attgccggcc cgccgaatgg
     3901 ggatcttgcg cagcgccatg ctcaggcgag ccttcccgga gcgatcccgc cagagagctt
     3961 gaccggctcg aactcgcctg caccgagggc gacgatttct gcgccacggc cgaagaagct
     4021 gctcatcatg ttttgcgcgc caaccagcag ggccatgacg agaacgacga agatcaaggt
     4081 gcggaagaag ccgttcaggt cgccaccgaa gatcagcacg ccacc
//
LOCUS       DAOSIT010000168         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19093_length_4125_cov_8.253808, whole genome shotgun sequence.
ACCESSION   DAOSIT010000168 DAOSIT010000000
VERSION     DAOSIT010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19093_length_4125_cov_8.253808"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..694
                     /locus_tag="PLL14_05325"
     CDS             149..694
                     /locus_tag="PLL14_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOG03376.1"
                     /translation="MPATRYTHTAIFIHWLMALLLIGLFSVGLYMSDLPLSPQKLKIY
                     SWHKWAGVTAFVLVLVRLAWRVGHPPPALPAAMPGWQKTAAHGLHHLLYLLMIAIPLS
                     GWLMSSAKGFQTVYFGILPLPDLLQKNKELGELLESVHTALNFTLAALVLAHIGAALK
                     HHLLDRDDILARMLPFLARKR"
     gene            721..1305
                     /locus_tag="PLL14_05330"
     CDS             721..1305
                     /locus_tag="PLL14_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG03377.1"
                     /translation="MEKPLTTFRLILATLAVAAAGSAAAAEFNQFQAGKSSLTFVSKQ
                     MGVPIDGSFRKFAASLAFDPARPAAATARLDLDLNSIDAGSRDANDEVVGKPWFNVKV
                     FPTATFVSSTVRPLGGDKYELVGKLTIKGKSQDVTAPFTFRQDGGNGVFDGAFVLKRL
                     EFGVGDGVWSDVSTVANEVQIKFHIVAAPAAIRK"
     gene            1332..1901
                     /locus_tag="PLL14_05335"
     CDS             1332..1901
                     /locus_tag="PLL14_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOG03378.1"
                     /translation="MKKQLGLIALASLFAAPALAAPETYLVDPNHTYPRFEYNHFGYS
                     NQIQRFNKTSGTIVFDRAAKTGSVDIVIDARSVDTGYALFNEHLQGEDFFDTAKYPTI
                     TYKSTAVRFDGDKPVAVEGLLTVKGITRPVTLSLGSFQTMPHPIVKKDALGANASARI
                     KRSEFNAGKYAPHVSDDVTLTIAVEALKQ"
     gene            2000..>2490
                     /locus_tag="PLL14_05340"
     CDS             2000..>2490
                     /locus_tag="PLL14_05340"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HOG03379.1"
                     /translation="MTGNATRTALSAEALDQLFVHARTHNTFAPEPIPEATLRQLYEL
                     MKWGPTSMNCQPARLVFVTTPEGKEKLAPALSPGNLDKTLAAPATVIIATDSLFYEHL
                     PTLFPAYDAKPMFAGNAELASSTAFRNSTLQGAYLIVAARGLGLDCGPMSGFDAGKLN
                     AAF"
     assembly_gap    2491..2590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2678..3877
                     /gene="purT"
                     /locus_tag="PLL14_05345"
     CDS             2678..3877
                     /gene="purT"
                     /locus_tag="PLL14_05345"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HOG03380.1"
                     /translation="MKIGTPLSPGALRVMLLGAGELGKEVIIALQRLGVEVIAVDRYA
                     NAPGHQVAHRAHVIDMTDGAALRALIEQERPHLIVPEIEAIATDTLLEIEAEQLAEVI
                     PTARAARLTMNREGIRRLAAEELGLATSPYRFADSLAELQSAIAGDIAYPCIVKPVMS
                     SSGKGQSLLRGPDDVSPAWDYAASGGRVNQGRVIVEGLIDFDYEITLLTVRARGAGGV
                     IETSFCEPIGHLQVAGDYVESWQPQAMSAVALEKARAIAAAVTGSLGGRGIFGVELFV
                     KGDQVWFSEVSPRPHDTGLVTLCSQHFSEFELHARAILGLPVDTHLREPGASAVIYGG
                     SDAKGIAFEGIAEALAVARSDLRLFGKPEAFRKRRMGVAVANGDDVAVARARAKLTAS
                     RVRPVAG"
     gene            complement(3918..>4125)
                     /locus_tag="PLL14_05350"
     CDS             complement(3918..>4125)
                     /locus_tag="PLL14_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03381.1"
                     /translation="QGKRMASWGCQHELDGKCSKINDLVCDPGMKGCVLAGRYFFLSS
                     DDKNQRLRDKQQRTASRSGDEPAD"
BASE COUNT          728 a         1450 c         1160 g          687 t
ORIGIN      
        1 catctgctgc cactgcacgt cgccatcggc gcggccggcc tcgattaccg gtcggaatgc
       61 ctgtaccagg gcatcgactc cgtagttctg gcgatggaca gctacgcgtt ctggcccact
      121 tccacccctg gctcataagg agaatggcat gcccgcgacc cgctacacgc acaccgcgat
      181 cttcatccac tggctgatgg ccctgctgct gatcggtttg ttcagcgtcg gtctgtacat
      241 gagcgacctg cccttgtcac cccagaagct gaagatctat tcctggcaca aatgggccgg
      301 cgtcaccgct ttcgtgctgg tactggttcg cctcgcctgg cgcgtcggcc acccaccgcc
      361 ggccttgccg gcagccatgc ccggctggca gaagacggcc gcgcatggct tgcaccacct
      421 gctctacctg ttgatgatcg ccatcccgct ttccgggtgg ctgatgagtt cggcaaaagg
      481 cttccagacg gtgtatttcg ggatcctgcc gctacccgat ctgctgcaga aaaacaagga
      541 actcggcgaa ctgctggaga gcgtacacac cgccctcaac ttcaccctgg cagccctggt
      601 cctggcacac atcggcgccg cgctcaaaca tcacctgctc gaccgcgacg acatattggc
      661 acgcatgctg cctttcctcg cccggaaacg ctgaccgtcc gcaatcgccg ccaaccccga
      721 atggagaaac cattgaccac ctttcgcctg atccttgcga cgctggccgt cgccgccgcc
      781 ggcagcgccg ctgccgccga gttcaaccag ttccaggccg gcaagagttc gctgaccttc
      841 gtttcgaaac aaatgggcgt gcccatcgac ggcagcttcc gaaagttcgc cgccagcctg
      901 gccttcgacc cggccagacc ggcagccgcc acggcccgcc tcgaccttga cctgaacagc
      961 atcgacgcgg gttccaggga cgccaacgac gaagtcgtcg gcaaaccctg gttcaatgtc
     1021 aaggtgtttc cgaccgcgac attcgtttcc tcgaccgttc gcccactcgg cggcgacaag
     1081 tatgaactcg tcggcaagct gaccatcaag ggcaagagcc aggacgtcac cgccccgttc
     1141 accttccgcc aggacggcgg caacggcgtc ttcgacggcg ccttcgtcct gaagcgtctt
     1201 gagttcggtg tcggcgacgg cgtctggtcc gacgtcagca ccgtcgccaa cgaagtgcag
     1261 atcaagttcc acatcgtcgc tgccccggca gccatccgca agtaagtccc ccaacgtcaa
     1321 ggagccttct catgaaaaag caactcggcc tcatcgccct cgccagcctg ttcgccgccc
     1381 cggcgctcgc cgccccggaa acctacctgg tcgaccccaa ccacacctac ccgcgcttcg
     1441 aatacaacca tttcgggtac tcgaaccaga ttcaacgctt caacaagact tccggcacca
     1501 tcgtcttcga ccgcgccgcg aagaccggct cggtcgacat cgtcatcgac gcccgctcgg
     1561 tcgataccgg ttatgcgctg tttaacgagc acctccaggg cgaagacttc ttcgataccg
     1621 cgaagtaccc gaccatcacc tacaagtcca ccgccgtcag gtttgacggc gacaagccgg
     1681 tggccgtcga aggcctgctc accgtcaagg gcatcacccg gccagtcacc ctgagcctcg
     1741 gttccttcca gaccatgccc cacccgatcg tcaagaagga tgccctgggc gctaacgcca
     1801 gcgcccggat caagcgctcc gagttcaacg ccggcaagta cgcgccgcat gtcagcgacg
     1861 acgtcaccct gaccatcgcc gtcgaagccc tgaaacagta agcatgcaga gcagcgggtc
     1921 cggtcgacgc cgccccgctt cgccccctcg ccgcttgccg aggccaaaac cctgcacaac
     1981 aaacccactg gagatatgaa tgaccggcaa cgccacccga acggccctca gcgccgaagc
     2041 tctcgatcaa ctcttcgtgc acgcgcgcac gcacaatacc ttcgcccccg aaccgatccc
     2101 cgaggcgacc ttgcgccagc tctacgagct gatgaagtgg ggcccgacgt cgatgaactg
     2161 ccagccggcg cggctggttt ttgtcaccac cccggagggc aaggaaaagc tcgccccggc
     2221 gctgtctccg ggcaacctcg acaagacact ggccgccccg gcaaccgtga tcatcgccac
     2281 cgacagcctc ttttacgaac acctcccgac cttgttccct gcctacgacg ccaagccgat
     2341 gttcgcgggc aatgccgaac tggccagcag caccgctttc cgtaacagta cgctgcaggg
     2401 tgcctacctg atcgtcgcgg ctcgcgggct gggtctcgac tgcggcccga tgtccggctt
     2461 cgacgccggc aagctcaatg ccgcattttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn gggcacgagc gatcaccgcg gcggcagcgc ttgacgccga cgccggtggc
     2641 gcatagattg cgtggcgccc aatccaagga aagccccatg aagatcggaa ccccgttgtc
     2701 ccccggcgcg ctgcgcgtca tgctgctcgg cgccggcgag ctcggcaagg aagtgatcat
     2761 cgccctgcaa cgactcgggg tcgaggtgat tgccgtcgat cgctacgcca acgcccccgg
     2821 ccatcaggtc gcgcaccgcg cgcatgtcat cgatatgacc gacggcgccg cgctgcgggc
     2881 gctgatcgaa caggaaagac cccatctcat tgtgccggag atcgaggcca tcgccaccga
     2941 taccctgctc gaaatcgagg ccgagcaact cgccgaagtc atccccaccg cccgtgcggc
     3001 caggctgacc atgaaccgcg aaggtatccg tcgcctggcc gccgaggaac tcggcctcgc
     3061 cacctcgcca taccgctttg ccgattccct ggccgaactg cagtccgcga tcgccggcga
     3121 tatcgcttac ccgtgcatcg tcaagccggt aatgtcgtct tccggaaagg ggcaatcgct
     3181 gctgcgcggt cccgatgacg tgtcgccggc ctgggattac gcggcgagcg gcgggcgggt
     3241 caatcagggc cgggtcatcg tcgaaggcct gatcgacttc gattatgaaa tcacgctgct
     3301 caccgtgcgt gcgcgcggcg ccggtggggt catcgaaacc tcattctgcg aaccgatcgg
     3361 acacctgcag gtcgccggcg actacgtcga atcctggcag ccgcaggcga tgagtgcggt
     3421 cgctctggag aaggcgcgcg ccatcgccgc cgccgtcacc ggcagcctcg gtgggcgcgg
     3481 catcttcggc gtcgaactgt tcgtcaaggg cgatcaggtg tggttctcgg aggtcagccc
     3541 gcgcccgcac gacaccgggc tggtgacgct gtgctcgcaa cacttctcgg aattcgagct
     3601 gcacgcgcgc gccatcctcg gcctgcccgt cgatacccac ctgcgcgagc cgggcgcttc
     3661 ggccgtgatc tacggcggca gcgacgccaa gggtatcgct ttcgaaggca tcgccgaagc
     3721 gctcgccgta gcgcgcagcg acctgcgcct gttcggcaaa cctgaggcct tccgcaaacg
     3781 ccgcatgggc gtggcagtgg ccaacggcga cgatgtggcc gtcgcgcggg cgcgcgcaaa
     3841 gctcacggca agccgggtca ggccggtggc cgggtagctc cccccggccg caaggcatgc
     3901 ctgctggcga aggcaactca gtccgcgggc tcgtcgcctg accggctcgc cgtccgctgc
     3961 tgcttgtcgc gcaggcgctg gttcttgtcg tcgctgctca ggaagaaata acggccggcc
     4021 agaacgcagc ccttcatccc cgggtcgcat accaggtcat tgatcttgct gcacttgcca
     4081 tcgagttcgt gctgacagcc ccaggaggcc atgcgctttc cttgc
//
LOCUS       DAOSIT010000169         4069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19408_length_4069_cov_12.649975, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000169 DAOSIT010000000
VERSION     DAOSIT010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19408_length_4069_cov_12.649975"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLL14_05355"
     CDS             complement(<1..146)
                     /locus_tag="PLL14_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOG03382.1"
                     /translation="MHNFDELVHAASAAFAATDDPDALEQIKATFLGRNGRVTELLKG
                     LAKLP"
     gene            complement(213..572)
                     /gene="rplT"
                     /locus_tag="PLL14_05360"
     CDS             complement(213..572)
                     /gene="rplT"
                     /locus_tag="PLL14_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOG03383.1"
                     /translation="MPRVKRGVTAHARHKKILALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRSLWIARINAAAREVGMKYSTFMNGLKKAQIEVDRKVLADLAVFD
                     KPAFAALAVQAKAQLAA"
     gene            complement(584..781)
                     /gene="rpmI"
                     /locus_tag="PLL14_05365"
     CDS             complement(584..781)
                     /gene="rpmI"
                     /locus_tag="PLL14_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOG03384.1"
                     /translation="MPKMKTKTGAKKRFKISPSGRIKRSHAYKRHILTKKDTKTKRQL
                     RGTTEVHHSDKAMVRAMMPYA"
     gene            complement(888..1409)
                     /gene="infC"
                     /locus_tag="PLL14_05370"
     CDS             complement(888..1409)
                     /gene="infC"
                     /locus_tag="PLL14_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOG03385.1"
                     /translation="MALQKAQRVNEEINAPEVRLVGLDGEQLGVMSVRAALALADEAG
                     VDLVEIAPMAQPPVCRIVDFGKFKYQEQKRQHEQKLKQKQVQVKEVKFRPGTDENDYQ
                     IKLRNMTRFLEEEDKVKVTLRFRGREMAHQDIGMRQIERIKSDLQDLAAVEQMPKMEG
                     RQMVMVLTPSRKK"
     gene            complement(1436..3355)
                     /gene="thrS"
                     /locus_tag="PLL14_05375"
     CDS             complement(1436..3355)
                     /gene="thrS"
                     /locus_tag="PLL14_05375"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG03386.1"
                     /translation="MPVVTLPDGSRREFAQPVTVAEVAQSIGAGLARAALAGRVDGRL
                     VDTSFAINHDAQLAIITEKDPEGIEVIRHSTAHLLAYAVKELFPAAQVTIGPVIENGF
                     YYDFAYQRPFTLDDLAAIEKRMSELARRDLPVQREVVARDAAIAFFKSIGEHYKAELI
                     EAIPQGEEVSLYREGAFVDLCRGPHVPSTGKLKVFKLMKLAGAYWRGDHRNEQLQRIY
                     GTAWVRKEDQDAYLHMLAEAEKRDHRKLGRQLDLFHLQDEAPGMVFWHPKGWTIWQEI
                     EQYLRRVLGANGYCEVKTPMIMDRSLWERSGHWQNYAEHMFTTASENRDYAIKPMNCP
                     GHIQIFNQGLRSYRDLPLRLAEFGSCHRNEPSGALHGIMRVRAFVQDDAHIFCTEAQV
                     LPETAAFIDLLQQIYADFGFTEILVKLSTRPDKRIGSDEVWDRAEAALAEALAAKNLG
                     YDLQPGEGAFYGPKIEFSLKDCLGRVWQCGTLQLDFALPQRLDAHYVGEDNDRHIPVM
                     LHRAILGSLERFIGILIENYAGAMPLWLSPMQAVVLNISEKQGEYAGQVAAQLRGAGL
                     RVEADLRNEKITYKIREHSLNKLPYQIVVGDKEMAANLVAVRTRGGRDLGQMPIEALT
                     TRLLDEIAARSSGTA"
     gene            complement(3585..>4069)
                     /locus_tag="PLL14_05380"
     CDS             complement(3585..>4069)
                     /locus_tag="PLL14_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOG03387.1"
                     /translation="PEQVEEGRRILLFSQFTSMLALIEKELKLAGLGYVILTGDTKDR
                     EEQVRRFQAGEVPIFLISLRAGGVGLNLTAADTVIHYDPWWNPAAENQATDRAHRLGQ
                     DKPVFVYKLIVAGSIEEKIIALQERKAELAARILSADRGVDAKFGSDDIAALFAPLPG
                     "
BASE COUNT          719 a         1265 c         1228 g          857 t
ORIGIN      
        1 ggcagtttgg ccagaccctt gagcaactcg gtcacccggc cgttcctgcc gaggaaagtg
       61 gccttgatct gctccagcgc atcagggtcg tcggtcgccg cgaaggctgc cgacgcggcg
      121 tggactagtt catcgaagtt gtgcatggcg ttgaagaggg tgttcgccga aaaaaaggag
      181 gctcacgcct cctttcttga gactcgtcgg acctaggctg cgagttgggc cttggcctgc
      241 acggccaaag cggcaaaagc cggcttgtcg aagacagcga gatcggcgag caccttgcgg
      301 tcgacttcga tctgcgcctt cttgagtcca ttcatgaagg tgctgtactt catgccgact
      361 tccctggccg cggcgttgat gcgcgcgatc cagaggctgc ggaactgacg cttgcgctga
      421 cgacggtcgc ggtaggcgta ctgtccggcc ttcattaccg cctgcttggc gacgcgatag
      481 acgttcttgc ggcggccgcg gtaacccttg gccagggcga gaatcttctt gtgacgcgcg
      541 tgcgccgtta caccacgttt gactcggggc atcgttgatc tccttaagcg taaggcatca
      601 tggcgcgaac catggccttg tcagagtggt gcacctcggt cgtgccgcgc aactggcgct
      661 tggtcttggt gtccttcttg gtcagaatgt gccgcttgta cgcgtgcgag cgcttgattc
      721 ggccactggg actgatcttg aagcgctttt tcgctcccgt cttggtcttc atcttgggca
      781 ttgaatgctc ctcttataaa catgccgcca ggtgtccccc gacgggggca cttcggaccc
      841 ggcaccactt gtttttgctg gcagcaggcc agcacttgaa tccgagccta cttcttcctg
      901 ctcggggtca acaccatgac catctggcga ccttccatct tcggcatctg ctcgactgcc
      961 gccaggtcct gcaaatcgga cttgatacgc tcgatctggc gcatgccgat atcctgatgg
     1021 gccatctcgc gaccacggaa gcgcaacgtg accttgacct tgtcttcttc ttcgaggaag
     1081 cgcgtcatgt tgcgcagctt gatctggtag tcgttctcgt cggtacccgg acggaatttg
     1141 acttccttca cctgcacctg cttctgcttg agcttctgct catgctgacg cttctgttcc
     1201 tggtacttga acttgccgaa atcgacgatc cggcagacag ggggctgggc catcggggca
     1261 atctcgacga gatcgactcc cgcctcgtcg gccagggcca gtgccgccct gacgctcatg
     1321 actcccagtt gctcgccgtc caaaccgacc agacggactt ccggtgcgtt gatctcttcg
     1381 ttaacgcgtt gcgccttttg cagtgcgatg ggtccactcc ttgtttcgac aaaaattaag
     1441 ccgtaccgct acttcgcgcc gcaatttcat cgagaagtcg cgttgtcagg gcctcgatcg
     1501 gcatttgccc caggtcccga ccaccgcgtg tgcgcacggc caccaggttc gctgccattt
     1561 ccttgtcacc aacgacgatt tggtacggca gcttgttcaa gctatgttct cgtattttat
     1621 aggtaatttt ttcgttgcgc aaatccgctt cgacccgcag gccggcacca cgcagctggg
     1681 ccgccacctg cccggcgtac tcgccctgtt tttcagaaat attcaacacc acggcctgca
     1741 tcggcgaaag ccacaatggc attgcaccgg cataattctc gatgaggatg ccgatgaagc
     1801 gctccagcga gccgaggatc gcccgatgca gcatcacagg aatgtgccgg tcgttgtcct
     1861 cgccgacgta gtgcgcgtcg agccgctgcg gcaacgcgaa atccagttgc agcgtgccac
     1921 actgccagac gcggccgagg cagtccttga gcgagaattc gatctttggg ccgtagaacg
     1981 cgccttcgcc gggctgcagg tcgtaaccca gattcttggc ggccagcgct tccgccaaag
     2041 ccgcttcggc gcgatcccac acttcgtcgg aaccgatgcg tttgtccggg cgcgtggaca
     2101 gcttgacgag gatttcggtg aacccgaaat cggcatagat ctgttgcaga aggtcgatga
     2161 acgccgccgt ttcaggcagc acctgcgctt cggtacagaa tatgtgtgcg tcatcctgga
     2221 cgaaggcgcg cacgcgcatg atgccgtgca acgcgcccga cggctcgttg cgatggcagg
     2281 aaccgaactc tgccagccgc agcggcagat cgcgatagct gcgcaagccc tggttgaaga
     2341 tctggatatg gccgggacag ttcatcggct tgatggcgta gtcgcggttt tcggaagcgg
     2401 tggtgaacat gtgctccgca taattctgcc aatgcccgga acgctcccag agcgagcggt
     2461 ccatgatcat cggcgtcttc acttcacaat agccgttggc gccgagtacg cgccgcaggt
     2521 attgctcgat ctcctgccag atggtccatc ctttcgggtg ccagaaaacc atgcccggcg
     2581 cctcgtcctg cagatggaag aggtcgagtt gccggccgag cttgcgatgg tcgcgcttct
     2641 cggcctctgc gagcatgtgc agataggcgt cctgatcttc cttgcgcacc caggccgtgc
     2701 catagatccg ctgcagctgc tcgttgcgat ggtcgcctcg ccagtaggcg ccggccagct
     2761 tcatcagctt gaacaccttc agcttgccgg tcgacggcac gtgcggacca cggcacagat
     2821 cgacgaaggc cccttcacga taaagcgaca cttcctcgcc ctgcggaatg gcctcgatca
     2881 gttcggcctt gtagtgctcg ccgatcgact tgaagaaggc aatggccgcg tcgcgtgcca
     2941 cgacttcccg ctgcaccggc aggtcgcgac gggcgagttc ggacatgcgc ttttcgatcg
     3001 ccgccagatc gtcgagcgta aacgggcgct ggtaggcgaa gtcgtaatag aagccgtttt
     3061 cgattaccgg cccgatggtc acctgcgccg ccggaaacag ttccttgacc gcgtaggcca
     3121 gcaggtgcgc tgtcgagtga cggatcactt cgatgccctc gggatccttc tcggtgatga
     3181 tggcgagttg ggcgtcgtgg ttgatcgcga aggaggtatc gaccagacgg ccatcgaccc
     3241 ggcccgccag cgccgcgcgc gccagcccgg caccgatcga ttgagccact tcggcaaccg
     3301 tgaccggctg cgcgaactcc ctccgggacc cgtcaggcag tgtgactaca ggcatggcgc
     3361 aacctcaaca aggaaatgga aaagccagag tcctgctctg gcttggtgga ttccggctgg
     3421 cacgaggaca cgatgacggc gaaacgttcc cgcatcccaa gcgcgagcgg gcgattctag
     3481 cacaccgcaa gcggcctgcc acacgcccca cggcaggcgt ttgccaacta ttccacagtt
     3541 gactcaacag cgcgagcggc gacaccggcg gctagccgcg ccgttcagcc gggcagcggc
     3601 gcgaacagtg cggcaatgtc gtcgctgccg aacttggcgt cgacgccgcg gtcggcggag
     3661 aggatgcgtg ccgcaagttc cgccttgcgc tcctggaggg cgatgatctt ttcctcgatg
     3721 ctgccggcga cgatcagctt gtacacgaat acgggcttgt cctgccccag gcgatgcgcg
     3781 cgatcggtgg cctggttttc ggctgccggg ttccaccagg ggtcgtagtg gatgaccgtg
     3841 tcggcggcgg tgagattgag accgacgccg ccggcgcgca ggctgatcag gaagatcggc
     3901 acttcgccgg cctgaaagcg acgcacctgc tcctcgcgat ccttggtatc gccggtgagg
     3961 atgacatagc ccaggccagc cagtttcagt tccttttcga tcagcgccaa catgctggtg
     4021 aattgcgaga acagcaggat gcgtcggcct tcctcgacct gctccggca
//
LOCUS       DAOSIT010000170         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19440_length_4063_cov_7.604042, whole genome shotgun sequence.
ACCESSION   DAOSIT010000170 DAOSIT010000000
VERSION     DAOSIT010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19440_length_4063_cov_7.604042"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLL14_05385"
     CDS             <1..233
                     /locus_tag="PLL14_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG03388.1"
                     /translation="RRILWFPLGQQALQVFPLLLLVQVVQFSVRMLVGADSPGISYFI
                     GPCLATLLWPLLTFVLLLPQHQPVDRDANRPL"
     gene            329..2224
                     /gene="mrdA"
                     /locus_tag="PLL14_05390"
     CDS             329..2224
                     /gene="mrdA"
                     /locus_tag="PLL14_05390"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOG03389.1"
                     /translation="MLARQSPLNASDRELHRFRFRVALAGFAVTVAFILLIARFVHLQ
                     VVQHDYYTTRAEDNRISLVPIVPNRGVIVDRHGTVLARNYSAFTLEITPSKVDDLDAT
                     IEGLGTVIEVLPKDRKRFRKLLEESKTFESLPIRSRLSEEEVARFAANRYRFPGVEVK
                     ARLFRQYPLGPIAAHAIGYISRINKRDLEVIEESEAAANYKGTDHIGKTGLEQRYEFQ
                     LHGETGYEQVEIDAGGRAIRNLSRTAPVQGNNLTLTLDTKLQEMAEKAFGDRRGALVA
                     IEPATGGILALVSTPTFDPNLFIDGIRTDDWDLLNNSPDKPLLNRALNGAYPPGSTFK
                     PFMALAALETGKRTAGQTISDPGFFNFGGHQFRDDKKGGHGSVDMYKSIVQSCDTYYY
                     MLANDMGIDSIARFMGQLGFGQRTGVDIDGESEGVLPSPEWKKRRFRRPEQQKWFAGE
                     TISIGIGQGYNAYTPIQLAQAMAAVANDGVMFRPHLVKYITDSRTGEQRMIEPEPLRI
                     LPWKRQNIETIKRAMIGVNTQGTGARAFAGAGYVSAGKTGTAQVFSLKGADYRSSSVK
                     KELRDHALFIAFAPADQPRIALAVLVENGGFGAQSAAPIARMVFDYYLLGKLPKGAAH
                     EDEVEGD"
     gene            2228..>2655
                     /locus_tag="PLL14_05395"
     CDS             2228..>2655
                     /locus_tag="PLL14_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG03390.1"
                     /translation="MLQQFLQRAWVRLVEHVDGPLLLITGLIMAIGLSTVYSATYDAN
                     NRALAQSLNMAVGLCAMWVVAQFPPQKLMRFGVPLYILGVILLVLVFLFGVKVNGARR
                     WLSLGFTRIQPSEVLKIAVPLMLAWYFHKHEAMLRFRHYAV"
     assembly_gap    2656..2755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2756..3115
                     /locus_tag="PLL14_05400"
     CDS             <2756..3115
                     /locus_tag="PLL14_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOG03391.1"
                     /translation="QTHLEFIPERHTDFIFAVFSEERGLLGNTTLVLLYLLLIARGLM
                     IAANASTLFARIIAGSVTLSLFTYVFVNMGMVSGILPVVGVPLPFMSYGGTALVTLSI
                     GIGILMSIQTHRMLVRK"
     gene            3292..>4063
                     /locus_tag="PLL14_05405"
     CDS             3292..>4063
                     /locus_tag="PLL14_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOG03392.1"
                     /translation="MVQKRGGAYYKDDGPGDEIPDNLDDIPDAQPRLEPLHRFANRPY
                     VVLGKAYEPHASLRPHQERGIASWYGKKFHGQKTSIGEPYDMFAMTAAHPTLAIPSYV
                     RVTNVSNGKSVVVRVTDRGPFHADRVIDLSYLAAYRLGYVDNGSTLVNVESILPEGAT
                     TMTYAQVMPVASPRRPVGTPAERDEIELLAAKLSLDKPPAPSMATGGPPPAFSPAAAS
                     ASSASMRSVFLQLGAFASAENAESLRSHLLRELDWLNEG"
BASE COUNT          719 a         1195 c         1231 g          818 t
ORIGIN      
        1 cgcgccgtat cctctggttt cctctggggc aacaggcctt gcaggtcttt ccgctattgc
       61 tgctggtgca ggtggtgcag ttcagcgtgc gtatgctggt tggcgccgat tctccgggca
      121 tcagctattt catcgggccg tgtctggcta cgctgctgtg gcctttgctg acctttgtcc
      181 tgctgctgcc gcagcatcaa ccggtcgacc gtgatgccaa ccggccgctc tgaaccctgc
      241 ggcgaggcgg tttcgcgcgc cgatcggcgc gttgtccgat cggcgcgcaa cacggtgtct
      301 gttaaccgcc gcgcctgaga ggttttccgt gcttgctcgt cagtcgccgc tgaacgcctc
      361 cgatcgcgaa ctgcatcgct ttcgctttcg tgtggccctg gccggttttg cggtcaccgt
      421 ggcctttatt ctgctgattg ctcgcttcgt gcatttgcag gtcgttcagc acgactacta
      481 tacgacccgc gccgaggata atcgtatctc gctggtgccg atcgtcccca accgcggcgt
      541 catcgtcgat cgccacggca cagtcctggc ccgcaactat tcggcattca cgctggaaat
      601 cacgccgtcg aaggtcgacg atctcgatgc aacgatcgag ggcctgggta cggtgatcga
      661 ggtcctgccc aaggaccgca agcgttttcg caagttgctg gaggagagca agaccttcga
      721 gagcctgccg attcgcagtc gactgagcga ggaagaagtc gccagatttg ctgccaaccg
      781 ctaccgtttt cccggtgtcg aggtcaaggc gcgcctgttt cgtcagtatc cgctgggtcc
      841 gatcgctgcg catgccattg gctacatcag tcgcatcaac aagcgtgacc tggaggtaat
      901 cgaggaaagc gaggcggcag ccaactacaa ggggaccgac catatcggca agaccgggct
      961 ggagcagcgg tacgaattcc agttgcatgg tgagacgggc tatgagcagg tcgagatcga
     1021 cgccggcggc agggcgatcc gcaacctctc gcggactgct ccggtacagg gcaataacct
     1081 gaccctgacc ctggacacca agttgcagga gatggcagaa aaagccttcg gcgatcgccg
     1141 cggtgcgctt gtggctatcg aaccagcgac tggcgggatc ctggcgctgg tctccacccc
     1201 gactttcgac cccaacctgt tcatcgacgg catccgcacc gacgactggg atctgctcaa
     1261 caattcgccg gacaagccgt tgctcaaccg ggcgctcaac ggcgcttatc cgccgggttc
     1321 gacgttcaag ccgttcatgg cactggccgc actcgaaacc ggcaaacgca cggccgggca
     1381 gacgatttcg gatccgggtt tcttcaattt cggcggtcac cagttccgcg atgacaagaa
     1441 aggtggccac ggcagcgtcg acatgtacaa gtcgatcgtg cagtcctgcg acacctacta
     1501 ctacatgctt gccaatgaca tgggtatcga cagtattgcc cgcttcatgg ggcaattggg
     1561 tttcggccag cgaaccggtg tggatatcga cggtgagtcg gaaggggtgt tgccgtcgcc
     1621 ggagtggaag aaacgtcggt tcaggcggcc ggagcagcaa aaatggtttg ccggagaaac
     1681 catctcgatt ggtatcggcc agggctacaa tgcctatacg ccgatccagt tggcacaggc
     1741 aatggccgcc gtggccaacg atggggtgat gttccgtccg caccttgtaa aatacatcac
     1801 tgatagccgt accggcgagc agcgcatgat cgagcccgag ccgctgcgca ttctgccgtg
     1861 gaagcggcag aatatcgaga cgatcaagcg ggcgatgatt ggcgtcaaca cccagggtac
     1921 gggcgcgcgc gctttcgctg gtgccggcta cgtctcggcc ggcaagaccg gtacggcaca
     1981 ggtcttcagc ctgaaagggg cggactaccg gtcgtccagc gtgaagaagg agctccgcga
     2041 tcatgcgttg ttcatcgctt ttgccccggc cgatcagccc aggatcgcgc tcgccgtgct
     2101 agtcgaaaac ggtggttttg gtgcccagtc ggcggcaccg atcgcgcgca tggttttcga
     2161 ctattatctg ctcggcaagc tgcccaaagg ggctgctcat gaagacgaag tggagggtga
     2221 ttagcggatg ttgcagcagt tcctgcagcg cgcgtgggtg cgtctggtcg aacatgtcga
     2281 tgggcctctg ctgctgatca ccggtttgat catggccatc ggcctgtcca ccgtttacag
     2341 tgccacctac gacgccaaca accgcgcgct ggcgcagagc ctgaacatgg cggtcgggct
     2401 ctgtgcgatg tgggtggtgg cacagttccc gccgcagaag ttgatgcgct tcggtgttcc
     2461 gctctacatt ctcggcgtga ttctgctggt ccttgtcttt ctcttcgggg tcaaggtcaa
     2521 cggggcgcgg cggtggttgt cgctggggtt tacgcgtatt cagccgtccg aggtgctgaa
     2581 aattgccgtg ccgctgatgc tggcctggta cttccacaaa cacgaagcga tgctcagatt
     2641 ccgccactat gctgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagac
     2761 gcacctcgaa ttcattccgg aacgccacac cgacttcatc tttgccgtgt tttccgaaga
     2821 gcgcgggctg ctgggtaaca ccactctggt gttgctctac ctgctgctga tcgcacgcgg
     2881 gctgatgatc gccgccaatg cgtccacgct tttcgcgcgg atcatcgccg ggtcggtgac
     2941 cctgagtcta tttacctacg tcttcgtcaa catgggcatg gtcagcggga ttctgccggt
     3001 agtcggggtg ccgctgccgt tcatgagcta cggcgggacg gcgctggtga ccctgtcgat
     3061 cgggatcggt atcctgatga gcatccagac ccaccgcatg ctggtcagga aatgaagcct
     3121 cgggagcctg ggctggcccg tcggtcgccc aacccaattg catggtggcc attggtgctg
     3181 tcactatcat ttctcacggc ctgcggaggg caacagacgc ggccccccga agcggcggga
     3241 tcttcgctgg agccggcaag agcggccgcg aaagcgccac ggaagtcgaa tgtggtgcag
     3301 aagcggggcg gggcatacta caaggacgat ggcccgggcg acgagatccc ggacaatctc
     3361 gacgacattc ccgacgcgca accacgactc gagccactgc atcgctttgc caaccggcca
     3421 tacgtcgttc tgggtaaggc ctatgagccg catgccagcc tcaggccgca ccaggagcgc
     3481 ggtattgcga gttggtatgg caagaagttt catggtcaga aaacttcgat cggcgagccc
     3541 tacgacatgt tcgcaatgac tgccgcccac ccgaccctgg cgattccctc gtacgtccgg
     3601 gtgaccaacg tcagcaacgg caagtcggtg gtcgttcgcg tcactgatcg tggtcccttc
     3661 cacgcggatc gtgttatcga cctttcttac ttggccgctt accggctcgg ctatgtggac
     3721 aacggcagta ccctggtcaa cgtcgagtcg atcctgccgg agggtgcaac gacgatgacc
     3781 tacgcgcaag taatgcctgt ggcgagtccg cggcgacctg tcggcactcc ggccgagcgc
     3841 gacgagattg aactgctggc ggcaaagctg agcctcgaca agccgcccgc tccatcgatg
     3901 gcgacgggtg ggccgccacc ggcattttct ccggctgccg ccagcgcctc ctcggcgagc
     3961 atgcgcagcg tgttcctgca actcggcgct tttgcaagcg ctgaaaacgc cgagagtttg
     4021 cgttcccatc tgcttcggga actcgactgg ctgaacgagg gcg
//
LOCUS       DAOSIT010000171         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19508_length_4052_cov_9.002252, whole genome shotgun sequence.
ACCESSION   DAOSIT010000171 DAOSIT010000000
VERSION     DAOSIT010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19508_length_4052_cov_9.002252"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..3759
                     /locus_tag="PLL14_05410"
     CDS             289..3759
                     /locus_tag="PLL14_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03393.1"
                     /translation="MNDVESATGNQPDLQDITADAEAARPHRVAMVDSEDRVFVNPAA
                     DRIAEIYASGLQERILSARLGLMQRATACSLEELLIATLDEVCALTGSSLGFYHFLGT
                     DQQTLTLQAWSTQTSRDYCRAEGQGRHYDVAEAGVWVECIRLRCPVIHNDYAALPHRR
                     GLPQGHASLLRELVVPIFRNGLIVAILGVGNKAEPYGEEDLTTVSQLADLAWDIAAAK
                     QADCQIRESQERLSGIVDAALDAIVTVDDVYQIVLFNAAAEQMFQCAAAEALGKSLDR
                     FIPERFRTIHRRQVMDFASTPVVSRPMGKTRQVCGVRAGGEEFPIEASISQVQVRGQR
                     LFTVALRDITERQRAQEALQKGQAFNVAVIDSLSAHVAVLDRKGKITAVNRAWRQFAA
                     DHGGATLATHPTGVDYRQACLSATGPCAAEALAAWQGIEAVLAGHSPHFEMEYSCHSP
                     DEEIWFQMKVQPLQGDCGGVVITHEDVTRSRRLANSLRSESQRLANIIEGTNVGTWEW
                     NVQTGEATVNERWAGIIGYTLAEISPVSIATWRHFAHPDDLEKSNDLAERHFAGELTH
                     YDCECRMRHKHGYWVWVRDRGKVMDWTADGKPLHMFGTHLDVTSEKLAEQALLEAIEF
                     NKQIIAGAQEGIVVYDRGLRCQVWNPYLENLTGLAAPQVIGSHPLELFPFLRETGSIA
                     RLEKALTGKLPGSTEFPFAVSQTGRTGWASGCAAPLLDTHGQIIGVIATVRDITETKR
                     AAEALREMNETLEARVDERTRQLAIATQIAESANRAKSFFLANMSHEIRTPMTAILGL
                     AELLNLDGVTTRQAERLKKMNHAARHLLSIIDDVLDLSKIEAGKLVLEEADCDVTSIV
                     DSVVEITSDRARQKRITLRTDCESIPGQLRGDPIRIRQALLNYVSNAIRFTERGSVTV
                     RVRCQESSDEHLLLRFEVQDTGIGIAADTIGRLFQSFEQADNSMTREYGGTGLGLAIV
                     KQIAQMMDGRAGVESCEGTGSTFWFTARLRRLIETSADLAGNSDTVPAVTVEAVEERM
                     RQEKRILLVDDERINREVILAILETIGLQADCAANGEEAVELAGSLSYDLILMDLQMP
                     RMDGLEATRRIRSLPINAQVPILALTGNVSTDVRSLCLAAGMNDFIAKPFTIDVLLAV
                     VDRWLLPETG"
     gene            complement(3850..>4052)
                     /locus_tag="PLL14_05415"
     CDS             complement(3850..>4052)
                     /locus_tag="PLL14_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03394.1"
                     /translation="FRRSQYAYVVYDALIKGSGVRAGVAVIHAGKTIANLSCKNPAAS
                     SLGPDFFAAAGLVTDDDDFEIP"
BASE COUNT          846 a         1257 c         1250 g          699 t
ORIGIN      
        1 acagcgcccg cgagcgctgc gccgccaatc ccgccagggc cgcgccgccg atgccacagg
       61 ccgtccacag gaaacggcgg cgccggttca tctcgtctct cgattcatga attgctctcg
      121 cccatccggc ggtatgccca caaagccctg gcggtgagtg taggggtatc aacgccagcg
      181 tcaagcaaaa cctgattcgc cgtaataggc atcgctgcgc aatcccttta tagttcgcag
      241 gtcggaacca ggtcattgac acctgttgcg ccgaccaggg agcgaataat gaatgacgtg
      301 gaaagcgcca ccggcaacca gcctgactta caggacatca ctgcagatgc tgaggcggcc
      361 cgccctcatc gggtggcgat ggtcgattcg gaggaccggg tgtttgtcaa cccggccgcc
      421 gatcgcatcg ccgagattta cgcgagcggg cttcaggagc gcatcctgtc ggctcgcctg
      481 ggcctgatgc aacgcgcgac ggcctgctcg ctggaggaat tgctgatcgc cacgctcgac
      541 gaagtgtgcg cgctgaccgg cagcagcctc ggcttctacc actttctggg aactgaccag
      601 cagacgctga cattgcaggc gtggtccacg cagacgtccc gagattattg cagggccgag
      661 ggtcagggcc ggcattacga tgtcgccgag gccggcgtgt gggtggaatg catccgcctg
      721 cgctgcccgg taatacacaa cgactatgcc gcgctgcccc acaggcgcgg tttgccgcag
      781 ggacacgcca gtctgctgcg cgaactggtg gtcccgattt tccgcaacgg actgatcgtc
      841 gccatcctgg gtgtcggcaa caaggccgaa ccctacggag aagaagacct gacgaccgtc
      901 agccagcttg ccgatctggc ctgggacatc gccgccgcca agcaagccga ctgccagatc
      961 cgggaaagcc aggaacgcct gagcggcatc gtcgatgccg ccctcgatgc gattgtgacg
     1021 gtagacgatg tgtatcagat cgtgttgttc aacgccgccg ccgaacagat gttccagtgt
     1081 gcggccgcgg aagctctcgg aaaatctctg gaccgattca ttccggagcg cttccgcacg
     1141 atacatcggc gccaggttat ggacttcgct tcgacacctg ttgtatcacg cccgatgggg
     1201 aagacccgcc aggtttgcgg ggtgcgggcc gggggagagg agttcccgat cgaagcgtcg
     1261 atttcccagg ttcaggtgcg tgggcagcgg ctattcacgg tcgcgttgcg cgacatcacc
     1321 gagcgtcagc gagcccagga ggccttgcag aaaggccagg cattcaacgt cgcggtgatt
     1381 gattcgctaa gcgctcacgt tgcggttctc gaccgtaagg ggaaaatcac cgcggtcaac
     1441 cgggcgtggc gacagttcgc ggcagatcac ggcggcgcca ctttggccac acacccaacc
     1501 ggtgtcgatt accgtcaagc ctgtctgtcc gccaccgggc catgcgcggc ggaagcgctg
     1561 gcggcgtggc agggcatcga agcggtgctc gcgggacact cgccgcattt cgagatggag
     1621 tacagctgcc attcgcccga cgaagagatc tggttccaga tgaaagtcca gccactgcag
     1681 ggagattgcg gtggcgtcgt gatcacccat gaagacgtca cccgcagccg gcgactggcc
     1741 aactcgctgc gttcggagag ccagcgcctc gccaacatca tcgaaggcac caacgtcggc
     1801 acctgggaat ggaacgtgca aaccggtgaa gccacggtca acgaacggtg ggcgggaatc
     1861 atcggctata ccctggccga aatctcgccg gtaagcattg ccacttggag acacttcgct
     1921 catccagacg atctggaaaa atccaacgac ctcgccgaac ggcattttgc cggcgagctg
     1981 acccactacg actgcgagtg tcgaatgcgt cacaaacacg gctactgggt gtgggttcgc
     2041 gatcgcggca aggtcatgga ctggaccgcc gacggaaagc cgctgcacat gttcggtacc
     2101 cacctcgatg ttaccagcga gaagcttgcc gagcaggcct tgcttgaagc aatcgagttc
     2161 aacaagcaga tcattgccgg ggctcaggaa ggcatcgtgg tgtatgaccg cggcctgcgc
     2221 tgccaggtct ggaatcccta tctggaaaac cttacgggct tagcggcgcc acaggtgatt
     2281 ggttcgcatc cccttgaact cttcccgttc ctgcgcgaaa ctggctcgat cgctcgcctc
     2341 gaaaaagcgc tcacgggcaa actgccgggt agcaccgagt tcccgtttgc ggtcagccag
     2401 acggggcgca ccggctgggc gtccggatgc gcagcaccgc tgctcgacac ccatgggcag
     2461 atcattggcg tcattgccac cgtacgcgac attaccgaaa ccaaacgtgc tgcagaagcg
     2521 ctacgggaga tgaacgaaac cctggaggca cgtgtcgacg aacgtacacg gcaattggcc
     2581 atcgccacgc agatcgccga atcggccaac cgggcgaaga gtttctttct ggccaacatg
     2641 agtcacgaaa tccgcacacc gatgaccgcc atccttggcc tcgccgaact gctgaacctg
     2701 gacggcgtca cgaccaggca ggccgagcgc ctgaagaaaa tgaaccacgc cgccaggcat
     2761 ctgctgagca tcatcgacga cgtgcttgac ctgtccaaaa tcgaagccgg caagctggtt
     2821 ctcgaagaag cggattgcga tgtgacgagc atcgtcgaca gcgttgtcga aatcacgtca
     2881 gatcgcgccc gacagaagcg gatcaccctg cgcacggact gcgagagcat cccgggccag
     2941 ctacgcggcg acccgatacg tatcagacag gccttgctca actacgtcag caacgccatc
     3001 cggtttacag agcggggcag cgtcaccgtc cgcgtccgtt gccaggaatc cagcgacgag
     3061 cacctcctgc tgcgtttcga ggtccaggac actggcatcg gcattgccgc cgacaccatt
     3121 gggcggcttt tccagagctt cgagcaggcc gacaactcga tgaccagaga atacggcggc
     3181 accggcctgg ggctggccat cgtcaagcag atcgcgcaga tgatggacgg ccgcgccggt
     3241 gtcgagagct gcgaaggtac tggcagtacc ttctggttta cggcacggct gcgccggctg
     3301 atcgaaacca gtgccgatct ggccgggaac agcgatacag tgccagccgt gacggtagag
     3361 gcagtggagg agcgcatgcg ccaagagaaa cgcatcctgc tcgtcgacga cgagcgtatc
     3421 aaccgggagg tcattctggc gatcctcgaa accatcggtc tgcaggccga ctgcgcggcc
     3481 aatggcgagg aagcggtcga actggccggc agcttgtctt acgacctgat cctgatggat
     3541 ctgcagatgc cgagaatgga cgggctggaa gcgacgcgca ggatccgcag tttgccgatc
     3601 aacgcgcagg tgccgatcct ggcgctgacc ggcaacgtca gcaccgacgt cagaagcctc
     3661 tgcttggccg ccggcatgaa cgacttcatt gccaagccgt ttacgatcga cgtgctgctt
     3721 gccgtagttg accgctggct gctcccagag acgggctgat ctgaacaccg gtcaacgctt
     3781 cgcctttccg gagcgatggc aaacctgcaa ctccgcgtct actcgaacgc ccgcggcgac
     3841 gccaaacggt tacgggatct cgaaatcgtc gtcatcggtt accaatccgg ctgctgcaaa
     3901 gaagtcggga ccaagggagg aggcggcggg attcttgcag gacaggttgg cgatggtctt
     3961 gccagcgtgt atcacggcaa caccggccct cacgccacta cccttgatca aggcatcgta
     4021 caccacatag gcatactggc tacgccggaa cc
//
LOCUS       DAOSIT010000172         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19556_length_4046_cov_7.986720, whole genome shotgun sequence.
ACCESSION   DAOSIT010000172 DAOSIT010000000
VERSION     DAOSIT010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19556_length_4046_cov_7.986720"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1078)
                     /locus_tag="PLL14_05420"
     CDS             complement(245..1078)
                     /locus_tag="PLL14_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG03395.1"
                     /translation="MQLSVAGHDCHVATGGQPFDPARSVWPPLLLIHGAANDCDAWHK
                     VAGGLAAAGCALLVPDLPGHGLSGGEPLSSIEALADWLPALLDSAGVAAAILVGHSMG
                     SLVALECAARYPQRVGGLALLGASAPMPVAEALLSSAATHPDRALRMMNDYSLTAPFQ
                     LRGGGGHGVWGPGVTLAIMRRSPPGVLAIDLANCNNYLHGLDAAAQVTCPTLLLVARR
                     DRMTPRRNLPPLQSALRQVQRAEIPDCGHAMMNERPEAVVEQLLKFLANPTTPTTDGG
                     A"
     gene            complement(1116..1325)
                     /locus_tag="PLL14_05425"
     CDS             complement(1116..1325)
                     /locus_tag="PLL14_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOG03396.1"
                     /translation="MLTEEQITENRATLHELQIEHHDLDQVIAHLMLNPPPDDLLIRR
                     LKKRKLLLKDRITLLEHLLEPDVLA"
     gene            1637..1957
                     /locus_tag="PLL14_05430"
     CDS             1637..1957
                     /locus_tag="PLL14_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03397.1"
                     /translation="MVYHIIVAFARERQYEFKFSHTELAAGSPEEARRWFDKEFADLE
                     CEPSNPMGKVLIIDKILNVARYGGEQRFIDGKDWATRFARYTALALGRDTVRIDVAAF
                     NIGY"
     gene            2100..2666
                     /gene="greB"
                     /locus_tag="PLL14_05435"
     CDS             2100..2666
                     /gene="greB"
                     /locus_tag="PLL14_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOG03398.1"
                     /translation="MNKAFTRETEDDGEQDEGADPALRLPQGTRNYITPDGHARIRSE
                     LDHLLRIERPQVVSVVQWAAANGDRSENADYIYGKRRLREIDRRIRFLTRRLDLAEVV
                     DPARRENTEQVFFGALVTLCDEQGMESTYQIVGVDETDSARGRISWVSPLARALLKSR
                     AGDVVRFQSPAGWRVVEVLAVEYPGSDT"
     gene            2791..>4046
                     /gene="htpG"
                     /locus_tag="PLL14_05440"
     CDS             2791..>4046
                     /gene="htpG"
                     /locus_tag="PLL14_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG03399.1"
                     /translation="MGAQKETLGFQAEVKQLLNLMIHSLYSNKEIFLRELISNASDAC
                     DRLRFEALNNAALYGDDAELKIRVTLDKEARTLSIADNGIGLSREEAVEHLGTIAKSG
                     TREFFSALTGDQAKDAHLIGQFGVGFYSSYIVADKVTVVSRRAGLESGQAVRWESSGE
                     GDFTVEVIERAQRGTEVTLHLRDGEDEFLSSWKVREIIRKYSDHITLPILMKKEEWNE
                     EQKAQVVTDQDETVNQASALWSRPKSEISDEQYNEFYKHVAHDFENPLAHVHARVEGK
                     QEYTQLLYIPSRAPFDLFERTARHGVKLYVRRVFIMDDAEQLMPLYLRFVRGVVDSND
                     LPLNVSREILQQSRDIETIRGGCVKKVLGLLEGLADSEDAAEKAKYATFWKEFGRVLK
                     EGAGEDFANKERIAKLLRFASTHADTS"
BASE COUNT          766 a         1180 c         1360 g          740 t
ORIGIN      
        1 tcaagggccg catcgtcggc tggctcgacg cccatccgct gtcggcgcag caagtgtttc
       61 agcggcagtg cgacaatagt tacggcgggc ggcatcagtt gacgttctcg gcaccaggct
      121 ggtgtcgatc ctggatgggc agcgacgcta tgcgcatgtg actgggctgt gcggcgacga
      181 agtcgcgccg caggttctcg gatccacggc gcctccggtc tgcgcgcggc cgagatgctc
      241 cccgtcaagc acctccgtcc gttgtgggcg tagtggggtt ggcgaggaac ttcaggagct
      301 gttcaacgac cgcttccggt cgctcgttca tcatcgcgtg accgcagtcg ggaatctcgg
      361 cgcgttgcac ctggcgcaga gccgattgca gcggcggcag gttgcgcctg ggggtcatgc
      421 ggtcgcgacg ggcgaccagc agcagggtcg gacaagtgac ctgcgccgcc gcgtcgagac
      481 cgtgcaggta gttgttgcag ttggcgaggt cgatggccag tacgccgggc gggctgcggc
      541 gcatgattgc cagtgtcacg ccgggtcccc agacgccatg cccgccgccg ccgcgcaact
      601 ggaacggcgc agtcagggaa tagtcgttca tcatccgcag ggcgcggtcg ggatgcgtcg
      661 cggcgctgct gagcagggct tcggcaaccg gcatcggtgc cgaagccccg agcagggcca
      721 gtccgccgac acgctgcgga tagcgtgcgg cgcactcgag cgccaccagc gaacccatcg
      781 aatgtccgac caggatagcg gcggccactc cggcgctgtc aagcagggcg gggagccagt
      841 cggccagtgc ctcgatgctg gacaggggct ccccgccgga taaaccgtgc cccggaaggt
      901 cggggacgag caatgcgcag cccgccgccg cgagaccgcc ggcgacctta tgccatgcgt
      961 cgcagtcgtt ggcggcacca tgaatcagca gcagcggcgg ccaaaccgag cgggcggggt
     1021 cgaaaggctg tccgccagtg gcgacgtggc agtcatgccc ggcgacagag agttgcatgc
     1081 gggtgcccag tgccggtcag cggcagtctt cggcgtcagg ccagaacatc cggttccagc
     1141 aagtgttcga ggagggtaat gcggtccttg agcagcagct tgcgtttttt gaggcgtcgg
     1201 atcagcaggt cgtctggggg tgggttgagc atcaggtggg cgatcacctg gtcgaggtcg
     1261 tgatgttcta tctgcagttc atgcagcgtg gcccggtttt cggtaatctg ttcttcggtc
     1321 agcatggtcg gcgcaatccc caaggttgta atgcggctcg ccgagccggg tttgcagacc
     1381 ccacaaggcg agcatgccag gaacgacggt caataaggcg ctgcgtgccc acccggcgac
     1441 gagtgccgtg agtgctgcgg cggcaaggcg cggtgggtca ggtcctatgg taggcgcgcc
     1501 cacggcaaaa aacaacggcg gcaggaatac cggcaataaa ctcgacgtgc gggctcggga
     1561 cttgcggcat ggggcgcatt tgcgaataga atagagggtg aaattgtaac cttgagatac
     1621 ccccgggagg aggcagatgg tttatcacat tattgttgcg tttgcgcggg agaggcagta
     1681 cgagttcaag ttctcccata ccgaacttgc tgcagggtct cccgaagaag cgcgccgctg
     1741 gtttgacaag gagttcgccg accttgagtg cgaacccagc aacccaatgg gcaaggtatt
     1801 gattatcgac aagattctca atgtcgcccg gtatggaggt gagcaacgtt tcatcgacgg
     1861 caaggactgg gcgacacgct ttgcccgcta cacagccctg gctcttgggc gcgacaccgt
     1921 tcgcatcgac gtcgccgcgt tcaatatcgg ctattgagtg gtcgtgcagt gcgcattgcg
     1981 gcgcaggttt caggacctgc gccgtttctt ttttccgcca ggacgtgcgg atccgttggg
     2041 ggatttggac tttcctcgcc cgacgcggat tccgcagtgc cgtcggcgac cgacaggcca
     2101 tgaacaaggc attcaccaga gagacggaag acgacggcga gcaggacgaa ggggctgatc
     2161 ccgccctgcg gctgccacag ggcacacgca actacatcac gccagacggg cacgcgcgca
     2221 tccggtcaga actcgatcat ctgttgcgca tcgagcggcc gcaggtggtg agtgtcgtgc
     2281 agtgggcggc agccaacgga gaccgctccg agaacgccga ctacatttac ggcaagcggc
     2341 gcctgcgcga aatcgaccgg cggatccgct tcctgaccag gcgcctggat ctggccgagg
     2401 tcgttgatcc ggcacgtcgc gagaacaccg agcaggtgtt ttttggtgcg ctggtgaccc
     2461 tctgcgacga gcaggggatg gaaagcacct accagatcgt cggcgtcgac gaaaccgact
     2521 cggcacgtgg ccgcatcagt tgggtgtcgc cgctggcgcg cgcgctgctc aagtcgcgtg
     2581 cgggtgatgt ggtgcgtttc cagagtccgg ccggctggcg ggtggtcgag gtgctggcgg
     2641 tcgagtatcc gggcagcgac acctgagcct gtttcaccga caagtcggca gggcttgaaa
     2701 tggtcgccgc cgcccccatt tcccgaagca ttgtgcgcaa ggtgcactgc ccgttcgttt
     2761 cacgttctca acttttttcc ggagtcttgc atgggtgccc agaaggaaac acttggtttt
     2821 caggcagaag tcaaacaact gctgaatttg atgattcact cgctgtacag caacaaggaa
     2881 atctttctgc gcgagttgat ctccaacgcg tccgacgctt gcgacagact gcgcttcgaa
     2941 gccttgaaca atgccgctct ctacggtgat gacgccgagc tgaaaatccg ggtgacgctc
     3001 gacaaggaag cccggacgct cagcattgcc gacaacggca tcggcctgtc gcgcgaggag
     3061 gcggtcgagc acctcggaac gatcgccaag tcgggcacac gcgagttctt ttcggcgctg
     3121 accggcgatc aggccaagga tgcgcacctg atcggccagt tcggcgtcgg cttctactcc
     3181 tcgtatatcg tggccgacaa ggtgaccgtg gtttctcgcc gggccggcct cgaaagtggc
     3241 caggcggtca ggtgggagtc ctccggcgag ggcgatttca cggtcgaggt gatcgagcgg
     3301 gcgcagcggg gaaccgaggt gaccttgcat ctgcgtgacg gcgaagacga attcctgtcg
     3361 agctggaagg tgcgcgagat cattcgcaag tactccgacc acatcacgct gccgatcctg
     3421 atgaagaagg aggaatggaa cgaggaacag aaggcgcagg tcgttaccga ccaggacgag
     3481 acggtgaacc aggcgtcggc gctttggagc cgtccgaagt cggagatcag cgatgagcag
     3541 tacaacgagt tctacaagca cgtcgcacac gatttcgaga acccgctggc gcacgtacac
     3601 gcccgggtgg agggcaagca ggaatacacc cagttgctgt acatcccgtc acgcgcgcct
     3661 ttcgatcttt tcgaacgtac cgcccgccac ggcgtcaagc tctacgtgcg gcgggtgttc
     3721 atcatggacg atgccgagca attgatgccg ctctatttgc gcttcgtccg cggggtcgtc
     3781 gattccaacg atctgccgct caatgtttcg cgtgaaatcc tgcagcagtc gcgcgacatc
     3841 gagacgatcc gtggtggttg cgtgaagaag gttctcggcc tgctcgaagg ccttgccgac
     3901 agcgaagatg ccgccgagaa ggcgaaatac gccactttct ggaaggaatt cgggcgtgtg
     3961 ctcaaggaag gtgccggcga agacttcgcc aacaaggagc gcatcgccaa actgttgcgg
     4021 tttgcctcga cgcatgccga tacctc
//
LOCUS       DAOSIT010000173         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19741_length_4019_cov_8.524218, whole genome shotgun sequence.
ACCESSION   DAOSIT010000173 DAOSIT010000000
VERSION     DAOSIT010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19741_length_4019_cov_8.524218"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLL14_05445"
     CDS             <1..396
                     /locus_tag="PLL14_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG03400.1"
                     /translation="RQKISLSYLEQLFGKLRRFGLVESVRGPGGGYCLARRTSEMTVT
                     DIIRAVDEPLDATQCGGRENCRDDERCMTHELWSTLNDKMYEFLSSVTLAELVDQQLR
                     KTGGHVAIIQDARRFKPRARANKSVSLTN"
     gene            410..1567
                     /locus_tag="PLL14_05450"
     CDS             410..1567
                     /locus_tag="PLL14_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOG03401.1"
                     /translation="MFAPVYLDHNASTPADPAVVEAMLPYLSAQFGNASSRHEYGRAA
                     RRAVDEARSKVAAAVGAHPTEVIFTSGGSEANNLLIKGAAACLKPGVLAVSAIEHPCV
                     LKPAEQLERRGWTLDRLAVGADGRIDASGFAVAMGRKPKIVSVMLANNETGVVQDLDL
                     LAERARFTGAWFHTDAVQAVGKIDVDFRRLNAAGVHALTVSAHKIGGPQGAAALVLDK
                     RIELEPLIAGGGHERGLRSGTENVAAIVGFGVACELAVQRRAALAQRLLKLRGELEHG
                     LRGCNATLFGQAAERLPNTVYFAIPDVDGETLVGHLDRAGYAVASGAACSSANPEPSH
                     VLRAMGVAPDLARGAVRVSLGYGNTAEQVAGFLSALQATVARLQGLTAMAV"
     gene            1624..2835
                     /locus_tag="PLL14_05455"
     CDS             1624..2835
                     /locus_tag="PLL14_05455"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOG03402.1"
                     /translation="MLKLPIYLDYSATTPVDARVAEKMIPYLVEKFGNPASRSHAFGW
                     EAEAAVEEAREEVARLVNADPKEIVWTSGATESNNLALKGAAQFLAGKGKHLVTVKTE
                     HKAILDTCRELERQGFEVSYLDVRDDGLLDLEVFKAALRPDTIVVSVMFVNNEIGVIQ
                     PIAEIGEICRARGILFHVDAAQATGKVVIDLAALKVDLMSFSAHKTYGPKGIGALYVR
                     RKPRVRLTAQMHGGGHERGFRSGTLATHQIVGMGEAYRLARLEMGAENERIRMLRDRL
                     LKGLSDIEEVYVNGDLERRVPHNLNISFAYVEGESLLMAIKDLAVSSGSACTSASLEP
                     SYVLRALGREDELAHSSIRFTLGRFTTEEEIDYAIKLLHDKIGRLRELSPLWEMVQEG
                     VDLSTVQWAAH"
     gene            2859..3254
                     /gene="iscU"
                     /locus_tag="PLL14_05460"
     CDS             2859..3254
                     /gene="iscU"
                     /locus_tag="PLL14_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOG03403.1"
                     /translation="MAYSIKVLDHYENPRNVGSFGKEEEGVATGMVGAPACGDVMKLQ
                     IKVGKGGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTIDQALDIKNTQIADELALPPV
                     KIHCSILAEDAIKAAVADYKKKHSEQVSV"
     gene            3291..3614
                     /gene="iscA"
                     /locus_tag="PLL14_05465"
     CDS             3291..3614
                     /gene="iscA"
                     /locus_tag="PLL14_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOG03404.1"
                     /translation="MAVTLSERAASHVANYMVKRGKGIGLRLGVRTSGCSGMAYKLEF
                     ADVAHPDDIQFESYGVRVLIDPKSLPYLDGTELDYAREGLNEGFRFNNPNVKDACGCG
                     ESFNV"
     gene            3685..>4019
                     /gene="hscB"
                     /locus_tag="PLL14_05470"
     CDS             3685..>4019
                     /gene="hscB"
                     /locus_tag="PLL14_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOG03405.1"
                     /translation="MDFNANHFSLFDLPPTFRLDLALLDRRYLEMQSKVHPDRFASAG
                     EAEKRLSLQWATRVNEAYQTLRKPLARARYLLHLAAYDLGSENNALMPADFLVEQMEW
                     REAVAEARAA"
BASE COUNT          778 a         1155 c         1335 g          751 t
ORIGIN      
        1 cggcagaaaa tctcgctttc gtatctcgaa caactgttcg gcaagctgcg ccgctttggc
       61 ctggtggaaa gcgtgcgtgg cccgggcggt ggttactgtc tggcgcgtcg gaccagcgaa
      121 atgacggtca ccgacatcat tcgcgccgtc gatgagccgc tcgatgccac gcaatgtggc
      181 ggccgcgaga actgccgcga cgacgagcgt tgcatgaccc atgagctgtg gtcgacgctg
      241 aacgacaaga tgtacgagtt cctgagttcg gtgacgctgg cggaactggt tgatcaacaa
      301 ctgagaaaaa ccggtggtca cgtggcgatc atccaggacg cgcggcgttt caagccacgc
      361 gcacgtgcca acaaatcggt ttccctcacc aattgaacgt caggtagcca tgttcgcgcc
      421 cgtctatctc gaccataacg ccagcactcc tgccgatccg gcggtggtcg aggcgatgct
      481 gccctacctc tcggcgcaat tcggcaacgc gtcgagccgg cacgagtacg gtcgggcggc
      541 gcggcgcgcg gttgacgagg cgcgcagcaa ggtggccgcc gcagttggcg cacatcctac
      601 cgaagtgatc tttaccagcg gcgggtcgga agccaacaat ctgctgatca aaggcgctgc
      661 cgcgtgtctg aaacccggtg tgctggcggt gagtgccatc gagcatccct gcgtgctcaa
      721 gccggcggag cagctggaaa ggcgcggctg gacgctggat cgcctggcgg tgggcgccga
      781 cggacggatc gatgccagcg gcttcgccgt ggccatggga cgcaaaccga agatcgtttc
      841 ggtgatgctg gccaacaacg aaaccggcgt ggtgcaggat ctggatctgc tggccgagcg
      901 tgcgcgtttc accggcgcct ggtttcacac tgacgccgta caggcggtgg gcaagattga
      961 cgtcgatttt cgccgtctga atgccgcggg cgtgcatgcg ctgactgtgt cggcacacaa
     1021 gatcggcggg ccgcagggcg ctgcagcgct ggtgctcgac aagcgcatcg agcttgagcc
     1081 cctgatcgcc gggggcggcc acgaacgtgg tttgcggtcg ggtaccgaga atgtagcggc
     1141 gatcgtcggc ttcggtgtcg cctgcgaact ggcggtgcaa cgtcgggcgg cgctggcgca
     1201 gcgcctgctc aagctgcgcg gcgaactcga acacgggctg cgcggctgca atgcgacgct
     1261 ctttggccag gccgccgaac gcttgccgaa taccgtttat ttcgcgatcc cggacgtcga
     1321 cggtgaaacc ctggtcggcc acctcgaccg tgccggatat gcggtggcca gcggcgccgc
     1381 ctgctcaagt gccaatccgg aaccttcgca tgtgctgcgg gcgatgggtg tcgcgcccga
     1441 tctggcacgc ggagcggtgc gtgtcagtct gggttacggc aatacggcag aacaggtcgc
     1501 cggttttctg agtgccttgc aggccaccgt ggcccgcctg caagggctga ccgcgatggc
     1561 cgtctagcgg tctcccggcc acatgaagaa ctgcataacc atggacaacc cggagcaaga
     1621 gcaatgttga aactgcctat ctatctcgat tattcggcca cgaccccggt ggatgcgcgc
     1681 gtggcggaga agatgattcc ctatctggtc gagaaattcg gcaatccggc ctcgcgctcg
     1741 cacgctttcg gctgggaagc cgaagcggcg gtcgaagaag cgcgcgaaga agtggccaga
     1801 ctggtcaatg ccgacccgaa agagatcgtc tggacgtcgg gcgcgaccga gtcgaacaac
     1861 ctggcactca agggagcggc acagttcctt gccggcaagg gcaagcatct ggtaacggtg
     1921 aagaccgagc acaaggccat tctcgatacc tgtcgggaac tcgaacgaca gggttttgag
     1981 gtcagctacc tcgacgtcag ggacgatggc ctgctcgacc tcgaagtgtt caaggcggcg
     2041 ctgcgtccgg acaccatcgt ggtgtcggtg atgttcgtca ataacgagat cggtgtcatc
     2101 cagcccatcg ccgagatcgg cgagatttgc cgtgccaggg gcatcctctt ccacgtcgat
     2161 gcggcgcagg cgaccggcaa ggtggtgatc gatctggcgg cactgaaggt cgatctgatg
     2221 agcttttcag cgcacaagac gtatggcccg aaagggatcg gtgccttgta cgtgcgtcgc
     2281 aaaccgcgcg ttcgcctgac ggcgcagatg cacggtggcg ggcacgagcg gggtttccgt
     2341 tccggcacgc tggcgacgca tcagatcgtc ggcatgggcg aggcgtaccg actggcgcgc
     2401 ctggaaatgg gcgccgagaa cgagcgcatc cgcatgctgc gcgaccgcct gctgaaaggc
     2461 ctgtcggaca tcgaagaggt gtatgtgaac ggtgacctcg aacggcgtgt gccgcataac
     2521 ctgaacatca gttttgcgta cgtcgaaggt gagtcgctgc tgatggcgat caaggatctc
     2581 gcggtgtcgt cgggatcggc gtgcacctcg gcgtctctgg aaccttcgta cgtgctgcgt
     2641 gccctcggtc gtgaggacga actcgcgcat agttcgattc gtttcacgct gggccgcttc
     2701 actaccgagg aggagatcga ctacgcgatc aagctcctgc acgacaagat tggccgcttg
     2761 cgcgaactct cgccattgtg ggagatggtg caggaaggcg tcgacttgag cacggtccag
     2821 tgggccgcgc attgatgaag cattgaagga gaaacagcat ggcatacagt atcaaggtcc
     2881 tggaccacta tgaaaatccg cgtaacgtcg gttccttcgg caaggaagag gagggcgtgg
     2941 cgaccggcat ggtcggtgcg cccgcctgtg gcgatgtgat gaagctgcag atcaaggtcg
     3001 gcaagggtgg cgtcatcgaa gatgccaagt tcaagaccta cggctgtggc tcggcgatcg
     3061 cttcgtcgtc actggtgacc gaatgggtca agggcaagac catcgatcag gctctggaca
     3121 tcaagaacac ccagattgcc gatgaactgg cattgccgcc ggtgaagatt cactgctcga
     3181 ttctcgccga ggatgccatc aaggcggcgg tcgccgatta caagaagaag cacagcgaac
     3241 aggtttccgt ctgacacggt ttgtcgcaac tacagcagga ggtctttcga atggctgtga
     3301 cgctttctga acgggcagcg agccacgtcg ccaattacat ggtcaagcgc ggcaagggta
     3361 tcgggttgcg cctgggtgtg cgcaccagcg gctgctcggg gatggcctac aagctcgaat
     3421 ttgccgatgt cgcccacccg gacgacatcc agttcgaatc ttacggggtc agggtgctga
     3481 ttgatccgaa gagtctgcct taccttgacg gcaccgaact cgattatgcg cgcgagggct
     3541 tgaacgaagg tttcaggttc aacaatccga acgtcaagga cgcctgtggc tgtggcgaaa
     3601 gcttcaatgt ctgagcgctg gcgtgatccg gtggctgacg gacttgctgc ttaagcgcgc
     3661 ccggatctga aggaggcgcg tcggatggat ttcaacgcga atcacttctc gctgttcgat
     3721 ttgccgccaa ccttccggct cgacctggcg ctgcttgacc gccgctatct tgagatgcag
     3781 tcaaaggtgc atccggatcg ctttgcgagt gccggcgagg ccgaaaagcg gctctcgctg
     3841 cagtgggcga cacgcgtcaa cgaagcctac cagaccctca ggaaaccgct ggcgcgggcg
     3901 cgctacctgc tgcatctggc agcgtacgat cttggcagcg agaacaatgc cctgatgccc
     3961 gcggacttcc tcgttgagca gatggaatgg cgcgaggcgg ttgccgaagc gcgcgctgc
//
LOCUS       DAOSIT010000174         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19742_length_4019_cov_8.110494, whole genome shotgun sequence.
ACCESSION   DAOSIT010000174 DAOSIT010000000
VERSION     DAOSIT010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19742_length_4019_cov_8.110494"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLL14_05475"
     CDS             <1..744
                     /locus_tag="PLL14_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HOG03406.1"
                     /translation="SLMLTSAGSAYLERCREILNLVEAAEAGLADERQEPRGLIRLSV
                     PLSFGLRHLSPLLMDFNVTYPDVVTEIDFTDRRVKLVDEGLDLAIRITRRLDPLDVAR
                     RIGSSRLQAIASPDYLRRHGEPKHPGELAEHECLIYLPTQHEGWPFLVNGATELFSVR
                     GRMRANNGDALLDAAMRGLGITAQPTFISAAAIEAGWVRPILTDYPMPELGIYALLPG
                     NRYVPQRVRGLVDYLAERIGSRPYWESAS"
     gene            complement(906..>1378)
                     /locus_tag="PLL14_05480"
     CDS             complement(906..>1378)
                     /locus_tag="PLL14_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03407.1"
                     /translation="TNQEIRQYTLVVTAITDTQVIYNNGSLITDLIGNPVKLADGTRY
                     TDAQHQIADYSLGKRWTARHRLTNAKGATYDSQIEYRVVARESVTLPAGTFDAFRVEG
                     MGWSQGERFGVEVYNLFWISPEVRRIIAHETRQRFANGKLVKHERYELTGYVQR"
     assembly_gap    1379..1478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1479..2215)
                     /locus_tag="PLL14_05485"
     CDS             complement(<1479..2215)
                     /locus_tag="PLL14_05485"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG03408.1"
                     /translation="MRRNILQGALAAGALTLLPCPGLAQTALNSLPRRSLIIGNSRYA
                     HAPLNNPANDARAIAEALKAVDFAVTLQLDASREAMLRAIDDYVGMLARDHAIGVFYF
                     SGHGTQLAWRNYLLPVDSEIRMLNDIPARGVELNTLLQGLTQAKNPMNVIILDACRDN
                     PFGQALPGEQKGLSQFDAPPGSLLAYATSPGNTASDGAGANGLYTENLLRELKSSDTK
                     IEDIFKRVRLEVRRRSDGRQIPWESTSL"
     gene            2475..3659
                     /locus_tag="PLL14_05490"
     CDS             2475..3659
                     /locus_tag="PLL14_05490"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOG03409.1"
                     /translation="MEKAILVLNAGSSSLKFSVFEVPASGEMRARAVGQVEGLGTAPR
                     LKVKDGDGQRIADVRWETTEVANHAQALDAIATLLRSRFEGQVLAGVGHRVVHGGADH
                     SAPLLITAPVMEKLDTFVPLAPLHQPHNLAAIRALQQARPDLPQVACFDTAFHRSQEM
                     VAQLYGLPYEYYERGIRRYGFHGLSYEYIAATLPSVAPEIAGGRVVVAHLGSGASMCA
                     MHHGRSVGSSMGFTALDGLLMGTRPGNMDPGIVLYLLQQEGLSAKQIEDLLYKRSGLL
                     GLSGIGNDMRVLEASDAPRARLAIDHFVYRISRELGGLAAVLGGVDALVFTAGIGENS
                     AHIRQLVCQQAQWLGLELDADANERGDTRISRVGSRMSAWVVPTNEELMIARHTRTLL
                     GL"
     gene            complement(3696..3974)
                     /locus_tag="PLL14_05495"
     CDS             complement(3696..3974)
                     /locus_tag="PLL14_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03410.1"
                     /translation="MNQEANDLGVATVMLVRLEKERLPRALDLKAKVDAGERLDDMDI
                     AFLERVFADTEEVKPYLARHPEYQDLAARMAHLYEQITARALDNEKSS"
BASE COUNT          648 a         1202 c         1298 g          771 t
ORIGIN      
        1 agcctgatgc tgacctcggc cggcagcgcc tacctcgaac gctgccggga aatcctcaac
       61 ctcgtcgaag ccgccgaggc cggtctggcc gacgaacgcc aggaaccgcg cggactgatt
      121 cgcctcagcg tgccgctgag tttcggtctt cgccacctgt cgccgttatt gatggatttc
      181 aacgtgacct atccggatgt cgtcaccgag atcgatttca ccgaccggcg ggtcaagctg
      241 gtcgacgaag gacttgatct cgcgatccgc atcacccgac gcctcgatcc tctcgatgtc
      301 gcgcggcgga tcggcagcag tcgtctgcag gcgatcgcgt caccggatta cctgcgccgc
      361 cacggcgagc cgaaacatcc cggcgaactg gccgagcacg agtgtctgat ctacctgccc
      421 acgcagcatg aaggctggcc gtttctggtg aatggggcga cggaactgtt ttcggtgcgc
      481 gggcggatgc gggccaacaa cggcgatgcg ttgctcgacg ccgcgatgcg tggtctggga
      541 atcaccgccc agccgacatt catcagcgcc gcggctatcg aggcgggctg ggtacggccg
      601 attctcaccg actatccgat gcccgaactc ggcatatacg cgttgctccc cggcaatcgc
      661 tacgttccac agcgggtgcg ggggctggtc gactatctgg ccgagcggat tggcagccgg
      721 ccgtactggg aaagcgccag ctagccatac gttccaaccc gttggccgct ggtagcgaac
      781 tgctcaagag cgtattcggc gcactctgaa cggtcagccc ggtctttgcc tcggccagga
      841 tgcttgagtt ccaggcgtca ccggggaagg tgacgccggc cgtctgcgac cgggatacca
      901 cccgcctagc gctgcacgta gccggtcaac tcgtagcgct catgtttgac gagcttgccg
      961 ttggcgaagc gttgccgggt ttcatgggcg atgatgcgac ggacttccgg ggagatccag
     1021 aacaggttgt atacctcgac cccgaacctt tcgccctggc tccagcccat tccttcgacc
     1081 cggaatgcgt cgaaagtgcc ggccgggagc gtgacgctct ccctggccac cacccggtac
     1141 tcgatctgcg agtcgtaggt ggcgcccttg gcgttggtca ggcggtggcg cgcggtccag
     1201 cgcttgccga gactgtagtc ggcgatctgg tgctgggcgt cggtgtagcg ggtcccgtcc
     1261 gccagcttga cggggttgcc gatcaggtcg gtgatcaggc tgccgttgtt gtagatcacc
     1321 tgcgtatcgg tgatcgcggt gaccaccagg gtgtactggc ggatctcctg gttggtcgnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cgatgtgctt tcccagggaa
     1501 tctgtcgacc gtcggagcgc cgccggactt ccaggcgcac gcgcttgaag atgtcctcga
     1561 ttttcgtgtc gctggacttg agttcgcgca gcaggttctc ggtatataag ccgttggcgc
     1621 cagccccgtc gctggccgta ttgcccggcg aggtggcgta ggcgagcaag gatccaggag
     1681 gggcatcgaa ctggctcaga cctttctgct cacctggcag ggcctgcccg aacggattgt
     1741 cgcgacaggc gtcgaggatg atcacgttca tcggattctt cgcctgcgtg agcccttgca
     1801 gcagcgtgtt gagctccacg ccgcgcgccg gaatatcgtt cagcatccgg atttcgctgt
     1861 cgacgggcaa caggtagttg cgccaggcga gttgcgtgcc gtgtccggaa aaatagaaca
     1921 cgccgatcgc atggtctctg gccagcatcc cgacgtaatc gtcgatggcc cggagcatcg
     1981 cctcgcgact cgcgtcgagc tggagcgtga ccgcgaagtc caccgccttc agggcctcgg
     2041 cgatcgcccg ggcgtcgttg gccgggttgt tgagcggcgc gtgcgcatag cggctgttgc
     2101 cgatgatcag cgaacggcga ggaagactgt tcagggccgt ttgcgccagc ccgggacacg
     2161 gcagcagcgt cagggcgcct gccgcgagcg ctccctggag aatgttgcgg cgcatgcggc
     2221 agggcgccgg cgccattggt acgtgcaccc cgttcatcgc atgccttgct gtctgtggtc
     2281 tgacggtgcg gtacgcgccg caggggagga aacatccagg gttctggcgc gagtatgcgt
     2341 agccctggtg gcggttgtca atcgggagtc gggccgggtg ttgttgccga actgcaggac
     2401 agccggtagg cgcgtagaat gacggctgtt tgcccgcgcg ccgcggcatg gtcccaagac
     2461 ctccggagat tgagatggaa aaagcgattc tggtcctcaa tgcgggctca tcgagcctga
     2521 aattttcggt atttgaggtg cctgccagcg gcgaaatgcg cgctcgcgcc gtgggtcagg
     2581 tcgaagggct gggcacggca ccgcgcctga aggtcaagga cggtgacggg cagcgcattg
     2641 ccgacgtgcg ttgggagact acggaggttg ccaaccacgc ccaggccctg gacgcgattg
     2701 cgacgctgct gcgcagccgt ttcgaggggc aggtcctggc gggggtcggg catcgtgtcg
     2761 tgcatggcgg ggccgatcac tcggcaccgt tgctgatcac cgcgccggtg atggagaaac
     2821 tggatacttt cgtgccgctg gcgccgctgc atcagccgca caacctcgcg gccatacggg
     2881 ccctgcagca ggcgcgaccc gatctgccgc aagtggcgtg tttcgatacc gccttccacc
     2941 gtagtcagga gatggtcgcg cagctctatg gtctgccgta cgaatactac gagcgtggca
     3001 ttcggcgtta cggttttcat ggcttgtcct acgagtacat cgccgcgacg ctgccaagtg
     3061 tggcgccgga aatcgccggt gggcgagtgg tggtggccca tctcggcagc ggcgccagca
     3121 tgtgtgcgat gcaccatggc cgcagcgtcg gcagttcgat gggattcacc gcgctcgatg
     3181 gtctgctgat ggggacgcgg ccgggaaaca tggatcccgg aatcgtcctc tacctcctgc
     3241 agcaggaagg actttcagcc aaacagatcg aagacctgct ctacaaacgt tccggcctgc
     3301 tgggcctgtc cggtatcggc aacgacatgc gcgtgctgga ggcgagcgat gctccgcgtg
     3361 cgcgcctggc catcgatcat ttcgtctacc gcatcagccg cgaactcggc ggcctggcgg
     3421 cggtcctcgg cggcgtcgat gcgctggtgt tcaccgccgg gatcggcgaa aacagcgccc
     3481 acattcggca actggtctgc caacaggcgc agtggctggg gctggaactc gacgcggacg
     3541 ccaacgaacg tggcgacacg cgcatcagtc gtgtgggcag caggatgtcg gcctgggtgg
     3601 tgcccaccaa cgaagagttg atgatcgcgc gccatacgcg cacgttgctc ggactttgat
     3661 cacctgtttc aagaagcacc gagcctgccg gacgttcagg aagatttttc gttatcgagc
     3721 gccctggcgg tgatctgttc gtaaagatgg gccatcctgg cagccaggtc ctgatactcg
     3781 gggtgtctgg caaggtaggg cttgacttct tccgtatcgg caaacactcg ttccagaaag
     3841 gcaatgtcca tgtcgtcgag ccgttcgccc gcgtctacct tcgctttcag atcgagcgct
     3901 cggggcagcc gctccttctc cagacgtacc agcatgactg tcgcaacccc caggtcattc
     3961 gcttcctgat tcattccctg ctccttttgg attgatgtgg actttcccgc cgacccacc
//
LOCUS       DAOSIT010000175         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19778_length_4013_cov_9.041435, whole genome shotgun sequence.
ACCESSION   DAOSIT010000175 DAOSIT010000000
VERSION     DAOSIT010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19778_length_4013_cov_9.041435"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLL14_05500"
     CDS             complement(<1..1276)
                     /locus_tag="PLL14_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOG03411.1"
                     /translation="MALLNKFAGRTTSPSRAPSPLPEKIAVLLRESRWLVLIVVASFL
                     ALSLWGYHRADPGWSHAVHATTLHNPAGRSGAWLADLMLYVFGVSAWWWIALLLAFVW
                     WGYRRLDGQRPTDRRPLYIALAGFVVLIVASSGLETLRFYTLKATLPVGPGGVMGLEL
                     RDFAVRYLGYTGATLTLLAMLILGWSIFSGMSWISAAERAGTLLEEAVVGVGKLFERW
                     QDRRIGREVARERKAVVDGEKKRGEAHEPSLPEKPETATPPPPKQGVKRSDAREPTLA
                     EGPQPSLADRPEPAATSTPLPPQDEQPDAALQPEPLPSEKAGAITTPPLALKVDKRIA
                     RERQPALFPDAVEGALLPPLHLLEPAPPQSDRIRPETLEYTSRLIERKLADFGVQVTV
                     TAAYPGPVITRYEFEPAVGVKGAQILNLAKDLA"
     gene            complement(1314..2024)
                     /locus_tag="PLL14_05505"
     CDS             complement(1314..2024)
                     /locus_tag="PLL14_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOG03412.1"
                     /translation="MNIQVEQPRRGPSLTLLHSIPIFAGVPDSQLEQIAKVAFHRRVT
                     RQTTIVRAGDSTDSLYVLISGGAKVLNSDEEGREVILTLLGPGEFFGEMGLIDGSPRS
                     ADVVATETCELLVITKTDFKRCLAENFDLCLNIMKSLVQRLREADRNIESLALLDVYG
                     RVAKLLLDFSEDEHGERVIRRRLTKQDIAKMIGASREMVSRVMKDLENRGYFRAEEGR
                     IVILEKRVPAPVTAPPQP"
     assembly_gap    2207..2306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2307..3020
                     /locus_tag="PLL14_05510"
     CDS             <2307..3020
                     /locus_tag="PLL14_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG03413.1"
                     /translation="HIHTARLTEKPFTLIGDSGAYTCDALIIATGASAQYLGLPSEET
                     YAGRGVSACATCDGFFYRNQPVAVIGGGNTAVEEALYLANIASHVTLVHRRDRLRSEK
                     ILQDKLFAKEQAGKVTIRWNCMLDEVLGDASGVTGMRIRNKLTDTTEELPLMGVFIAI
                     GHRPNTELFVGQLAMEGGYLVTQCGRQGNFTATSIAGVFAAGDVQDHIYRQACTSAGS
                     GCMAALDAERYLDSLGLAG"
     gene            3093..3656
                     /locus_tag="PLL14_05515"
     CDS             3093..3656
                     /locus_tag="PLL14_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOG03414.1"
                     /translation="MNLCDRPDAEDVAAFLAAVDGATPLRKFDRVSIEPPRPSPRPRQ
                     RELDEAAVIGEMLHAPLGIDDWLDLGGADSFLRNGLPRTLLRDLRRGRWSIQDHVDLH
                     GMNRHEAHAQVALFIAEALAAGKRCLRIVHGRGHGSPGREAILRQWVKVWLGRCQEVL
                     AFCHAPPNDGGDGALWVLLKAGGKLRP"
     gene            complement(3670..4008)
                     /locus_tag="PLL14_05520"
     CDS             complement(3670..4008)
                     /locus_tag="PLL14_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG03415.1"
                     /translation="MKKIEAIIKPFKLDEVREALSEIGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVVSDTAVENAIDAIVKAARTGKIGDGKIFVSTVEQVVRI
                     RTGEVDEAAI"
BASE COUNT          701 a         1236 c         1241 g          735 t
ORIGIN      
        1 gcgccagatc cttggccagg ttgaggatct gcgccccctt gacgcccacc gccggttcaa
       61 attcataacg ggtgatgacc ggccccgggt aggccgcggt caccgtgacc tgcacgccaa
      121 aatccgcgag tttgcgctcg atcagccgcg aggtgtattc aagggtttcc gggcggatgc
      181 ggtcactctg cggcggcgcc ggttcgagca gatgcagggg cggcagcagg gcaccctcga
      241 cggcgtcggg aaagagcgca ggttgtcgct cgcgggcgat gcgcttgtcg actttcagcg
      301 caagtggtgg tgtcgtgatc gcacctgctt tctcactcgg gagaggctct ggctgcaggg
      361 ctgcgtcagg ctgctcgtct tgcggcggca gcggtgtcga ggtggctgcc ggctcgggcc
      421 tgtcggcgag ggacggctgc ggcccttcgg cgagcgtcgg ttcccgagcg tcgctgcgct
      481 tgaccccctg cttcggtggt ggaggggtgg ccgtctctgg tttctcgggt agggagggtt
      541 catgcgcctc gccccgtttc ttctcgccat cgaccaccgc tttgcgttcg cgcgcgactt
      601 cgcggccgat gcgccggtcc tgccagcgct cgaacagctt gccaacgccg acaaccgcct
      661 cttcaaggag tgttccggcg cgttccgcag cgctgatcca cgacatgccg gagaagatgc
      721 tccagccaag gatcagcatt gccaacaggg tcagcgtcgc gccggtatag cccaggtagc
      781 gcaccgcgaa atcgcgcaac tccaggccca tcacgcctcc cggaccgacc ggcagcgtcg
      841 ccttgagggt gtagaaacgc agggtttcga ggccactgct ggcgacgatc agcaccacga
      901 atccagccag agcaatgtac agcgggcggc ggtcggttgg tcgctgaccg tcgagtcggc
      961 ggtagcccca ccagacgaag gcgagcagca gggcgatcca ccaccacgcc gagacgccga
     1021 agacgtacag catcaaatca gccagccagg caccgctgcg accagccggg ttatgcagcg
     1081 tcgtggcatg cacggcgtgc gaccagccag ggtcggcccg atggtaaccc cacagggaga
     1141 gcgcgagaaa actcgcgacg acgatcagga ccagccagcg cgattcccgc agcagaaccg
     1201 cgatcttctc cggcagcgga ctcggcgctc tggacggaga cgtcgtcctg ccggcaaatt
     1261 tgttcagcaa agccatcagt ttcggacggg gagtgggaat gggaaaatgg ggttcagggc
     1321 tgcggtgggg cggtgactgg ggcggggacg cgcttttcga gaatgacgat gcgaccttct
     1381 tcggcccgga agtagccgcg attttccaga tccttcatca cccggctgac catttcgcgc
     1441 gaggccccga tcatcttggc gatatcctgt ttggtcagcc gccgacggat cacgcgttcg
     1501 ccgtgctcgt cttccgagaa gtccaggagc aacttggcga cgcgaccata gacgtcgagc
     1561 agcgccagac tttcgatatt gcggtccgct tcacgcaggc gctgcacgag gctcttcatg
     1621 atgttcaggc agaggtcgaa gttctcggcc aggcagcgct tgaagtcggt cttggtgatc
     1681 accagcagtt cgcaggtttc ggtggcgacc acgtctgctg aacgcgggct gccatcgatc
     1741 agtcccatct cgccgaagaa ctctccgggg ccgagcaggg tcagaatcac ctcgcgtccc
     1801 tcttcgtcgc tgttgagcac cttggcgccg ccgctgatca ggacgtacag cgaatcagtg
     1861 ctgtcgccgg cgcgaacgat ggtggtctgg cgcgttaccc gacgatgaaa cgcgactttt
     1921 gcgatctgtt cgagttgcga atcgggtacg ccggcaaaaa tcgggatgct gtgcaacaaa
     1981 gtcaggctgg gacctctcct gggttgctcg acctgaatgt tcatgggcca atctttcatc
     2041 aagtgaagtg atttctatca aaggcttgcg ccgggattat aaggcacttt gccgcatttc
     2101 gaaggaaacc cgccacgaac ttggcgccgg gagcttgcgt tttgccggtt ataatttttc
     2161 catcacccac caccagagcg aacaagatga accaagcaac ccgccannnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnncaca tccacaccgc gcggctgacc gaaaagccct
     2341 ttaccctgat cggcgattcc ggcgcttaca cctgcgacgc gctgatcatc gctaccgggg
     2401 cttcggccca atacctgggc ctgccttccg aagagactta tgccggtcgt ggggtttccg
     2461 cctgtgccac ctgcgacggt ttcttctacc gcaaccagcc ggtagccgtc attggcggcg
     2521 gcaacacggc ggtcgaggaa gcgctctacc tggcgaacat cgccagtcac gtcaccctcg
     2581 tacatcgacg cgacaggctg cgcagtgaaa agatcctgca ggacaaactg tttgccaagg
     2641 aacaggccgg caaggtcacg atccgctgga actgcatgct cgacgaggtg ctcggcgatg
     2701 cctccggagt taccggcatg cgcattcgaa acaagctcac cgacaccacc gaggaactgc
     2761 cgctgatggg cgtcttcatt gccatcggcc accggccgaa caccgaactt ttcgtcggcc
     2821 agctcgccat ggaaggcggc tatctggtca cgcagtgcgg acgccaaggc aatttcacgg
     2881 cgacctcaat cgccggcgtc tttgccgctg gcgacgttca ggatcacatt taccggcagg
     2941 cctgtacttc ggcgggcagc ggctgcatgg cggcgctcga tgccgagcgc tacctcgaca
     3001 gcctcggtct ggccggctga gagtggggcg ggttcccagc gagcgaggcc ctcgacatgc
     3061 gtgcgcgacc gacacgagga gcgatgccgg cgatgaacct ctgcgatcga cccgatgccg
     3121 aagatgtggc cgcgtttctc gccgcggtcg atggggcgac gccgctgcgc aagtttgatc
     3181 gcgtcagcat tgaaccgccc aggccaagcc cgcgaccacg gcagcgcgaa ctcgacgaag
     3241 cggcggtcat cggcgaaatg ctgcatgctc ctctgggcat cgacgactgg ctcgacctcg
     3301 gcggtgccga ctcttttctg cgcaacggac tgccacgcac cctgctgcgt gatctgcgtc
     3361 gcgggcgctg gagtatccag gatcacgtgg acctgcacgg catgaaccgg cacgaggcgc
     3421 acgcgcaagt ggcgctgttc attgccgaag cactggctgc gggcaagcgc tgcctgcgta
     3481 tcgtacacgg ccgcgggcat ggttctccgg gtcgggaggc gatcctgcgt cagtgggtca
     3541 aggtctggct cggtcgctgc caggaggtgc tggcgttttg tcacgcgccg cccaacgacg
     3601 gcggtgatgg tgccctgtgg gtgttgctga aggccggcgg caaactccgc ccataggcgc
     3661 cccggctaat cagatcgctg cctcgtcgac ttcgcccgtg cggatgcgga ccacctgctc
     3721 gacggtgctg acgaagatct tgccgtcacc aatcttgccg gtacgtgcgg ccttgacgat
     3781 ggcgtcgatg gcgttctcca ctgccgtatc ggaaacgacg atctcgatct tcaccttggg
     3841 caggaaatca accacatact ccgccccacg atagagttcg gtgtggccct tctggcggcc
     3901 gaatcccttc acctcggtga ccgtcaggcc ggtcacgcca atctcggaca aggcctcacg
     3961 cacttcgtcg agcttgaatg gtttgatgat cgcttcaatt tttttcatgg cct
//
LOCUS       DAOSIT010000176         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_19847_length_4002_cov_9.183177, whole genome shotgun sequence.
ACCESSION   DAOSIT010000176 DAOSIT010000000
VERSION     DAOSIT010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19847_length_4002_cov_9.183177"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="leuC"
                     /locus_tag="PLL14_05525"
     CDS             complement(<1..1271)
                     /gene="leuC"
                     /locus_tag="PLL14_05525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOG03416.1"
                     /translation="MSQTLYEKLWQDHVVHQQADGTALIYIDRHLVHEVTSPQAFEGL
                     KLAGRKPWRVSSIVATADHNTPTDHWDRGIEDPVSRLQVETLDANIREVGALAYFPFR
                     DPRQGIVHVVGPESGATLPGMTVVCGDSHTSTHGAFACMAHGIGTSEVEHVLATQCLL
                     QKRSKTMLLQVDGVLGKGVTAKDVALAIIGRIGTAGGTGYAIEFAGSAIRGLSMEGRM
                     TLCNMAIEAGARLGLVAVDEVTIDYLRGRPFAPQGEQWERAVVAWRQLHSDPDARFDG
                     VVELSAADIRPQVTWGTSPEMVVPVDSCVPDPASEQDPTRREGMQRALRYMGLRPNLP
                     IREIWLDKIFIGSCTNSRIEDLRAAAGIVRGRKVAGNIRQALVVPGSGLVKQQAESEG
                     LDQVFLAAGFEWREPGCSMCLAMNADRLEPG"
     gene            complement(1323..>1996)
                     /locus_tag="PLL14_05530"
     CDS             complement(1323..>1996)
                     /locus_tag="PLL14_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03417.1"
                     /translation="YAWAVPEALPSESAEAHLPVAKIIRQARVRALFAACFAMSAAHG
                     ALYVFYSIHLSDHHYSKFLVGCLWSLGVVAEIVVFFFMAGLLRRFGLRTLLLVSFSAA
                     VLRFLLIAWGADWLSVMIFAQLLHGLTFGAYHAAAIAAVNRWFPGHCQARGQALYSSL
                     SFGAGGLVGSLLSGWTWGAWGAAPTYAISSAFALVGLVVVAAWVKKADLDACAGADSV
                     DRGVT"
     assembly_gap    1997..2096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2097..2353)
                     /locus_tag="PLL14_05535"
     CDS             complement(<2097..2353)
                     /locus_tag="PLL14_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03418.1"
                     /translation="MHALPYWRLSGYYFFYFAFIGAFSPYFGLYLQSLSFSAWDIGLL
                     MSQMQLMRLFAPYLWGALADRLAQRVAVVRLAGLLSLLGFST"
     gene            complement(2401..2652)
                     /locus_tag="PLL14_05540"
     CDS             complement(2401..2652)
                     /locus_tag="PLL14_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOG03419.1"
                     /translation="MQVEHHDLHHEFPEYLDFIHALQASSEQFGHLYDQYQNLTREVE
                     RLEEEDLPVDDFTIENMKKHRVWLKDQMYRMLVAQKSGG"
     gene            complement(2732..3835)
                     /gene="aroC"
                     /locus_tag="PLL14_05545"
     CDS             complement(2732..3835)
                     /gene="aroC"
                     /locus_tag="PLL14_05545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG03420.1"
                     /translation="MSGNTIGTLFAVTSFGESHGPAIGCVVDGCPPGLMISAVDIQGE
                     LDRRKPGTSRHVTQRREPDAVEILSGVFEGRTTGAPIALLIRNQDQRSKDYGNLAETF
                     RPGHADYVYTRKYGFRDHRGGGRSSARETAVRVAAGAIARKWLAERYGVSIRGWMSQL
                     GPIEIPFVNAAEIDGNPFFAPNAAIVPQLEEYMDRLRKSGDSVGAKITVTANGVPAGW
                     GEPVYGRLDAEIAYAMMSINAVKGVEIGAGFACVAQKGSEHGDEMTPQGFLSNHAGGV
                     LGGISSGQEVIVNLAIKPTSSIRLPRRTVTLDGQVSMVETHGRHDPCVGIRATPIAEA
                     MLALVLIDQALRHRAQCADVSSNTPPIPGKVAG"
BASE COUNT          788 a         1316 c         1183 g          615 t
ORIGIN      
        1 tctccgggtt cgaggcggtc ggcattcatc gccagacaca tcgaacagcc cggctcgcgc
       61 cactcgaagc cggcagcgag gaaaacctgg tccagtccct cgctttcggc ctgttgcttg
      121 accaggccag agcccggaac gaccagggcc tgccggatgt tgccggccac cttccggccc
      181 ctgacaatcc cggcggcagc tcgcagatcc tcgatccgcg agttggtgca cgaaccgatg
      241 aaaatcttgt cgagccagat ctcccggatc ggcaggttcg ggcgcagccc catgtagcgc
      301 agggcacgct gcatgccttc gcgccgcgtc ggatcctgct cactggccgg gtcggggacg
      361 caggaatcga ccggcaccac catctccggc gaggtgcccc aggtcacctg cggcctgatg
      421 tccgcggccg acagttcgac gacaccatca aatcgggcgt cgggatcgct gtgcaactgg
      481 cgccaggcca cgacggcgcg ctcccactgc tcgccctgcg gagcgaacgg acgaccgcgc
      541 aggtagtcga tcgtcacctc atcgacggcc accagaccca ggcgagcacc cgcttcgatg
      601 gccatgttgc agagcgtcat ccgcccctcc atcgacaagc cgcgaatcgc gctgccggca
      661 aactcgatag cataacccgt accgccggcg gtaccgatgc ggccgatgat ggccagcgcc
      721 acgtccttcg ccgtcacgcc tttgccgagc accccatcga cctgcagaag catcgtcttc
      781 gaccgcttct gcagcaggca ctgcgtggcc agcacgtgtt cgacctcgga cgtgccgatg
      841 ccgtgcgcca tgcacgcgaa agcgccgtgc gtactggtat gcgagtcccc gcagacgacg
      901 gtcatgcccg gcaacgtcgc accgctttcc gggccgacga catgcacgat gccctggcgc
      961 gggtcccgga acgggaaata agccagcgca ccgacttcac ggatattggc atcgagcgtc
     1021 tcgacctgca gacgcgacac cgggtcctcg atgccccggt cccagtggtc ggtgggtgtg
     1081 ttgtggtcgg ccgtggcgac gatcgaggac acccgccaag gcttgcggcc agccagcttg
     1141 agaccttcga aggcctgcgg gctggtcact tcgtgcacca gatggcggtc gatgtagatc
     1201 agtgcggtcc catccgcttg ctgatggacg acatggtcct gccaaagctt ctcgtacaag
     1261 gtctgcgaca tcgatgaata tcctgcgagg ttcgcccccg gtgggcaaca tgaacggctg
     1321 gattatgtca caccgcgatc aacactgtca gcgccagcgc atgcgtccag gtcagccttc
     1381 ttcacccacg cggcgacgac aacgagtccc accagcgcaa aggccgaact gatggcatag
     1441 gtcggggccg cgccccaggc accccaggtc cagccgctga gcaggctgcc gaccaggccg
     1501 ccggcgccga acgacaggct cgaatacagg gcctgcccac gcgcctggca atgaccaggg
     1561 aaccagcggt tgacggcagc gatggcagcg gcgtgatagg cgccaaaggt cagcccgtgc
     1621 aacaactgcg cgaagatcat caccgacagc caatcggcgc cccaagcgat caacaggaag
     1681 cgcagcacgg cagcagaaaa gctgaccagc agcagagtac gcaaaccaaa ccggcgtaac
     1741 agcccggcca tgaagaaaaa caccacgatc tcggcgacga cgccgagcga ccacaggcaa
     1801 ccgaccagga acttgctgta gtgatgatcg gaaagatgga tcgagtagaa gacgtacaag
     1861 gcgccgtgcg ccgccgacat cgcaaaacac gcggcgaaca gtgccctgac gcgcgcttga
     1921 cggatgatct tggccaccgg cagatgcgcc tcggccgact ccgacggcag cgcctcggga
     1981 accgcccaag cgtagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcg
     2101 agaaacccag caggctgagc aggcccgcca gacggacgac agccacccgc tgcgccagcc
     2161 gatcggcgag ggcaccccac agataagggg cgaacagccg catcaactgc atctgcgaca
     2221 tcaacagacc gatatcccag gccgaaaacg acaaggactg gagataaagc ccgaaatacg
     2281 gtgaaaacgc gccgatgaag gcgaaataga agaagtagta cccggagagg cgccagtagg
     2341 gcagagcatg catccggcaa ttctaccgga tgcacccgac cccgccgggt gacgaacggc
     2401 tcagccgccg ctcttctgcg ccaccagcat ccggtacatc tggtctttca gccacacccg
     2461 atgtttcttc atgttttcga tcgtgaagtc gtcgaccggc aggtcttcct cctcgagacg
     2521 ttcgacttcc ctggtcaggt tctggtattg atcatacaag tggccgaact gctcgctcga
     2581 cgcttgcagt gcgtgaatga aatcgaggta ttcagggaac tcatggtgaa gatcgtgatg
     2641 ctcaacttgc atgatcgtgg cctctggcaa atcccggccc gccctatgcg cgaccggacc
     2701 gcgcgattat aaccattggc aaggccaccc gtcatcctgc caccttaccc ggaatgggtg
     2761 gggtgttgct cgacacatcg gcgcactggg cacgatgtcg caaggcctga tcgatcaaca
     2821 ccagggccag catcgcctcg gcgatcggcg tcgcgcggat gcccacgcag gggtcgtgtc
     2881 gaccgtgcgt ttcgaccata gaaacctgtc cgtcgagcgt caccgtgcgg cgcggcaggc
     2941 ggatgctaga agtcggcttg atggccaggt tcacgatcac ttcctggccg ctggagatgc
     3001 cgccaagcac gcctccggca tggttgctga ggaaaccctg cggcgtcatc tcgtcaccat
     3061 gttcactgcc cttctgggca acgcaggcaa agccggcccc gatctcgacg cccttgacgg
     3121 cgttgatgct catcattgca taggcgatct ctgcatcgag acgaccatag accggctcgc
     3181 cccagcccgc cggcacgccg ttggccgtca ccgtaatctt cgcacccacc gaatcgccag
     3241 acttgcgcag acggtccatg tactcctcca gttgcggcac gatcgccgcg ttcggggcga
     3301 aaaaaggatt gccgtcgatc tcggccgcgt ttacgaaggg aatctcgatc ggcccgagct
     3361 ggctcatcca gccccggatg ctgaccccgt agcgttcggc cagccatttg cgggcgatcg
     3421 ccccggccgc cacacgcacc gccgtttcac gcgccgagga gcggccgccg ccgcgatggt
     3481 cacgaaagcc gtacttgcgg gtatagacgt aatccgcgtg tccgggccga aaagtctcgg
     3541 caagattgcc gtagtccttg ctgcgctgat cctgattgcg gatcagcagg gcgatcggcg
     3601 cgcccgtcgt gcggccctcg aaaacgccgg acaggatctc gaccgcgtcg ggttcgcgac
     3661 gttgcgtcac atgccgcgag gtccccggct tgcggcggtc gagttccccc tggatgtcca
     3721 ccgcgctgat catcaggccg ggcgggcagc catcgaccac gcagccgatc gccggaccgt
     3781 gtgactcacc aaacgaagtc acggcaaaca gggtgccgat ggtgttgccg gacatggccg
     3841 cctctggggt gttaagatga ccgtgaagtc taccacagcc ccgcgcaccc cccggcgcac
     3901 ggcacggttc aggacatcgc gcgaccaggg ctggagaccg gacaccaacg ggcttaccag
     3961 gccgtacaga acattcatcg atggaggctc gccagatgac cc
//
LOCUS       DAOSIT010000177         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20089_length_3966_cov_9.795449, whole genome shotgun sequence.
ACCESSION   DAOSIT010000177 DAOSIT010000000
VERSION     DAOSIT010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_3966_cov_9.795449"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLL14_05550"
     CDS             complement(<1..1127)
                     /locus_tag="PLL14_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG03421.1"
                     /translation="MLIDFFLHLKASRLPVSIKEFLALLEALQKHVIEHSLDDFYVLA
                     RAILVKDETHFDKFDRAFGEYFKGVEALPGMEVLIPEEWLRQAVKKHLTDAEREKLEK
                     LGWDQLMETFRQRLAEQKERHSGGSKWIGSGGSSPFGHGGTHPEGIRVGGRSENRSAV
                     KVWEKREFRNLDDSIELGTRNIKVALRRLRRFAREGAAEELDLEGTIAGTARNAGWLD
                     LQLRPERHNAVKVLLFLDIGGSMDDHIRVCEELFSACRSEFKHLEHYYFHNCVYDYLW
                     QDNRRRHSERIPTPEVMHKYGNDYKLILVGDASMSPYELVQPNGSVEFNNAEPGATWL
                     RRLAENWPHTIWLNPESEHSWPYRQSTSLIHNLLGGRMFPLT"
     gene            complement(1148..1990)
                     /locus_tag="PLL14_05555"
     CDS             complement(1148..1990)
                     /locus_tag="PLL14_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG03422.1"
                     /translation="MRFSGSESYVATGDLTLAVNAAITLQRPLLIKGEPGTGKTMLAE
                     EVAAALGLPLFEWHIKSTTKAQQGLYEYDAVSRLRDSQLGEARVADIGSYIVKGVLWQ
                     AFECETPSVVLIDEIDKADIEFPNDLLRELDRMEFFVYETRQKVRARHRPVLFITSNN
                     EKELPDAFLRRCFFHYIKFPDKETMTRIVDVHFPGLKPTLLREALEVFFELREIPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSKDQKTAIPPLHGALLKNEQDVHLFERLIFMA
                     RHNR"
     gene            complement(2126..3325)
                     /locus_tag="PLL14_05560"
     CDS             complement(2126..3325)
                     /locus_tag="PLL14_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG03423.1"
                     /translation="MTTLSKPLAGLTVIELGSLIAGPFAARILAEFGAEVIKIESPDG
                     GDPLRKWRKLYESTSLWWYVQARNKKSVTVNLKHPAGREIVHKLVGEADIVVENFRPG
                     VLEKLGLGWETLSAINPALVMLRLSGFGQTGPMAQQPGFGAIGESMGGLRYVTGFPDR
                     PPVKTGISIGDSIAALWGVIGTLLALRHKEQAGGRGQVVDVALYEAVFAMMESLIPEF
                     DVFGFVRERTGNIMPGITPSNTHTTSDGKHVTIGGNGDAIFRRLMLAIGRDDLANDAQ
                     LADNAGRDTRRDELYAAIDAWTAVHAEGEVLRILTAADVPVSRVFSAADMFADPQYLA
                     RQMIESAKLPDGKTFRIPGIVPKLSVTPGSTEWLGPGLGEHTDETLRRLGYSAAEIAA
                     LHDAGTV"
     gene            complement(3440..>3966)
                     /locus_tag="PLL14_05565"
     CDS             complement(3440..>3966)
                     /locus_tag="PLL14_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG03424.1"
                     /translation="EGAFRDWSYALAKNEFGGVEIDGGPWLKLPNGIIIKDSIADAFL
                     QQILLRPAEYDVICTTNLNGDYISDALAAQVGGIGIAPGANISDQYACFEATHGTAPK
                     YAGLDKVNPGSLILSAEMMLRHLGWIEAADLIVTAMEAAIGDKVVTYDFARLMEGATE
                     VSCSAFGDAMIARM"
BASE COUNT          754 a         1226 c         1217 g          769 t
ORIGIN      
        1 gtcagcggaa acatccgccc gccgagcaga ttgtggatca gcgaagtcga ttgccggtaa
       61 ggccatgaat gttccgactc ggggttcagc cagatggtat gtggccagtt ttcggccagg
      121 cggcgcaacc aggtcgcgcc gggttcggca ttattgaact cgacactgcc gttcggctgt
      181 acgagttcgt agggactcat cgatgcgtcg ccgacaagga tcaatttata gtcgttgccg
      241 tacttgtgca tcacttctgg cgtgggaatg cgctcgctgt ggcgacgccg gttatcctgc
      301 cacagataat cgtagacaca gttgtggaag taatagtgtt cgaggtgctt gaattcgctg
      361 cggcaggccg agaagagttc ctcgcagacg cggatgtgat cgtccatcga accgccgata
      421 tcgaggaaaa gcagcacctt gacggcattg tgacgctccg gccgcagttg cagatcgagc
      481 cagccggcgt tgcgggcggt accggcgatg gtgccttcga gatcgagctc ctcggcggct
      541 ccttcacgcg caaagcggcg caggcgacgc agggcgacct tgatgttgcg tgtgcccagt
      601 tcgatgctgt cgtcaagatt gcggaattca cgcttctccc agaccttgac cgctgaccgg
      661 ttttcgctcc gcccaccgac gcggatgccc tcggggtgcg tgccgccatg accgaacggc
      721 gaactgccgc cactgccgat ccacttgctg ccgccggagt gacgctcctt ctgttcggcg
      781 aggcgctgcc ggaaagtctc catcagctgg tcccagccga gtttttccag tttctcccgt
      841 tcagcgtcgg tgagatgctt cttcaccgcc tgccgcagcc attcttccgg aatcagcacc
      901 tccatacccg gcagcgcctc gacccccttg aagtactcgc cgaacgcgcg gtcaaacttg
      961 tcgaagtgcg tctcgtcctt gaccaggatg gcgcgcgcca gaacgtagaa atcgtcgaga
     1021 ctatgttcga tgacgtgctt ctgcagcgct tcgagcaggg cgagaaactc cttgatcgac
     1081 accggcaacc ggctcgcctt gaggtgcagg aagaaatcga tgagcatcgt cggccttgat
     1141 cagccggtca gcggttgtgg cgggccatga agatcaggcg ctcaaagaga tggacgtcct
     1201 gctcgttttt cagcaaggcg ccgtgcagcg gcgggatggc cgtcttctgg tccttgctgc
     1261 gcagtgcttc cggtggaatg tcctcggcaa gcagcagctt gagccagtcg agcaactccg
     1321 acgtcgaggg cttcttcttc aggccgggaa tttcgcgcag ttcgaagaac acctccagcg
     1381 cctcgcgcag caacgtcggc ttcaggccgg gaaaatgcac gtcgacgatg cgggtcatcg
     1441 tctccttgtc gggaaacttg atgtagtgga aaaaacagcg ccgcaggaag gcgtcgggca
     1501 gttccttctc gttgttcgag gtgatgaaca gcaccggccg atggcgggca cgcaccttct
     1561 ggcgggtttc gtagacgaag aactccatgc gatcgagttc gcgcagcaga tcgttgggaa
     1621 attcgatgtc ggccttgtcg atttcgtcga tcagcaccac cgaaggcgtt tcacattcga
     1681 aggcctgcca gaggacaccc ttgacgatgt aactgccgat gtctgcaacc ctggcctcgc
     1741 caagctgcga atcccgcagg cgcgagacgg cgtcgtattc atagagcccc tgctgcgcct
     1801 tggtcgtcga cttgatgtgc cactcgaaca gcggcagccc aagcgcagcg gcgacctcct
     1861 cggccagcat ggtcttgccg gtacccggct cacccttgat caacagtggg cgctgcaggg
     1921 tgatcgcggc gttgaccgcc agagtcagat cgccggtagc aacgtaactc tcggaaccgg
     1981 aaaagcgcat ggtggtattc ctttcgaaga cctgctgatt ctacctgtaa agaccaggac
     2041 cagtgtcagt accacggcgg cgccgacagc ggcccaacac ccggagatgg acccatacgg
     2101 cgcgcgaggt tcgatacccc caccctcaga ctgttcccgc atcgtgcaac gcagcgatct
     2161 cggcagccga atagccgagt cgccgcaggg tttcgtcggt gtgttcgccg agtccggggc
     2221 ccagccattc ggtgctgccc ggagtgaccg acagcttcgg cacgattccc gggatcctga
     2281 acgtcttgcc gtcgggcagc ttggccgatt cgatcatctg cctggccaga tactgcgggt
     2341 cggcaaacat gtcggcggca gagaacaccc gcgaaaccgg gacgtcggca gcggtcagga
     2401 tgcgcagcac ctcgccttcg gcatgtacag cggtccaggc gtcgatggcg gcgtacagtt
     2461 catcgcggcg tgtgtcgcgc cccgcgttgt cggcaagttg cgcgtcgttg gccagatcgt
     2521 cgcggccgat ggccagcatc agccgtctga agatggcgtc gccattgcca ccgatcgtga
     2581 catgcttgcc gtcactggtg gtgtgcgtat tggaaggagt gataccgggc atgatgttgc
     2641 cggtacgctc gcgcacgaag ccgaaaacgt cgaattccgg gatcagcgac tccatcatcg
     2701 cgaagaccgc ctcgtacagg gcgacatcga ccacctgccc ccggccaccc gcctgttcct
     2761 tgtgacgcaa ggccagcagc gtgccaatca ctccccacaa cgcggcgatc gaatcgccga
     2821 tcgaaatacc ggtcttgacc ggtggccgat ccgggaaacc ggtgacatag cgcaagccgc
     2881 ccatcgactc gccgatggcg ccgaaacccg gctgctgcgc catcgggccg gtctggccga
     2941 aaccggaaag acgcaacatg accagcgcgg ggttgatcgc cgacagcgtt tcccaaccca
     3001 gcccaagctt ttcgagcacg ccggggcgaa agttctcgac cacgatgtcg gcttcgccga
     3061 cgagcttatg gacgatctcg cgcccggccg gatgcttgag attcaccgtc accgatttct
     3121 tgttgcgcgc ctgtacgtac caccacagcg aggtactttc gtagagcttg cgccatttgc
     3181 gcaaggggtc gccgccgtcg ggagattcga tcttgatcac ctcggcgccg aattccgcca
     3241 gaatgcgggc ggcaaatggg ccggcaatca gactgccgag ttcgatgacc gtcaatccgg
     3301 ccaggggttt gctcaatgtc gtcatgggaa tggagatccg tcctgctgtg gcacccgatg
     3361 caccgctgac gcgcgcgccg aggagaaagc acaaagcccc cttgcggggg ccttgtcagt
     3421 gtggcaggtg ggcccttgcc tacatgcggg caatcatcgc gtcgccgaaa gcagagcacg
     3481 acacctcggt cgccccttcc atcaggcgcg cgaagtcgta ggtaaccacc ttgtcgccaa
     3541 tcgccgcttc cattgccgtg acgatcaggt cggcggcttc gatccaaccc agatgacgca
     3601 gcatcatttc ggcggagagg atcagcgaac caggattcac cttgtccagt ccggcgtatt
     3661 tcggtgccgt gccgtgcgtg gcctcgaagc aggcatactg gtcggaaatg ttcgcgccag
     3721 gggcaatacc aataccgccg acctgcgccg ccagcgcgtc ggagatgtaa tcgccgttga
     3781 ggttggtggt acagatcacg tcgtattcgg ccggacgcag caggatctgc tgcaggaagg
     3841 catcggcgat cgagtccttg ataatgatgc cgttcggcag cttgagccac ggaccaccgt
     3901 cgatctcgac accaccgaat tcgttcttgg ccagcgcgta cgaccagtca cggaaggcgc
     3961 cttcgg
//
LOCUS       DAOSIT010000178         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20112_length_3962_cov_7.658562, whole genome shotgun sequence.
ACCESSION   DAOSIT010000178 DAOSIT010000000
VERSION     DAOSIT010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20112_length_3962_cov_7.658562"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLL14_05570"
     CDS             complement(<1..794)
                     /locus_tag="PLL14_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOG03425.1"
                     /translation="MNRYPLWKYVIVAVAILLGLVYTLPNFFGESPAVQVSSAKATLK
                     VDDKTRERVAITLQTAGVEHSGIFLDSNGVKVRLRTTDSQLQAKDLLEKQFNPDASNP
                     QYVVALNLLSSSPQWLSSLHALPMYLGLDLRGGVHFLLQVDMKGALTKRLDSISGDLR
                     SLLRDKTIRHAGISREGERVIVRFRDSEARNKGRMVLEDNQSDLLLADQGEGEDLKLV
                     ATLKPEAVKRIQEFAIRQNMTTLHNRINELGVAEPVIAQQGADRIVV"
     gene            complement(853..1182)
                     /gene="yajC"
                     /locus_tag="PLL14_05575"
     CDS             complement(853..1182)
                     /gene="yajC"
                     /locus_tag="PLL14_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG03426.1"
                     /translation="MLISTAHAQQAAAAADPTGGLMQLLPIILMFVVLYFLMVRPQMK
                     RAKEHKALVEGLAKGDEVVTGGGIAGRVTRVSDNFLALEIADTVEIQVQKQAVTLVLP
                     KGTLKSL"
     gene            complement(1236..>2267)
                     /gene="tgt"
                     /locus_tag="PLL14_05580"
     CDS             complement(1236..>2267)
                     /gene="tgt"
                     /locus_tag="PLL14_05580"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG03427.1"
                     /translation="PAFMPVGTYGTVKAMTPRDLIETGAQICLGNTFHLWLRPGLEVI
                     NAHAGLHRFMNWHGPLLTDSGGFQVFSLGALRKITEEGVRFQSPINGDRLFLTPEESM
                     HIQKVLDSDIVMILDECTPYPATHDEAATSMRLSLRWARRSRTQHDHLENSNALFGIV
                     QGGMYEDLRDESLAALVDIGFDGFAIGGLSVGEPKADMARILAHTAPRLPQHKPRYLM
                     GVGTPEDLVAAVQAGIDMFDCVLPTRNARNGHLFTRHGDVRIRNARYKHDPGPLDASC
                     ACYTCRNFSRAYLHHLNRVGEILAAQLATLHNLHYYQELMGDLRTAISTGTLARSVES
                     FHSARARSN"
     assembly_gap    2268..2367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2368..>3962)
                     /locus_tag="PLL14_05585"
     CDS             complement(<2368..>3962)
                     /locus_tag="PLL14_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOG03428.1"
                     /translation="LWMLQTRNGKRTGSAMVRIAMEMLRQGIIDEKEALRRVNPERLN
                     ELLHPVFDPAAIKKARSIAHGLPASPGAATGQIVFFADEAEEWVRKGKTVILVRQETS
                     PEDLRGMSVAKGILTARGGMTSHAAVVARGMGKCCVSGAGAVHVDYHARTMSIGSETY
                     NEGDWLSLDGSTGEVYLGQVPTKEAELTGDFGTLMELADRYKRLGVRANADTPEDAKA
                     ARNFGAKGIGLCRTEHMFFEGDRIKAVREMILADDEAGRRRALAKLLPMQRGDFEGLF
                     REMSGLAVTIRLLDPPLHEFVPHDEENQRIMAHEMNVPLGVIKMRVDDLHEFNPMMGH
                     RGCRLGITYPEITEMQTRAILEAGLNMKAKGFDVKAEIMIPLVGTVREFNSQARIIRQ
                     TAKAVFAERGEKLDFLLGTMIETPRAALIADSIGPQAEFFSFGTNDLTQMTMGFSRDD
                     AGKFLPSYLKDGMYEQDPFQSIDQKGVGLLVKMAVEKGRAARPGIKLGVCGEHGGDPA
                     SVEFFHRTGLDYVSCSPFRVPLAR"
BASE COUNT          681 a         1162 c         1246 g          773 t
ORIGIN      
        1 acgacgatgc ggtcggcgcc ctgctgggcg attaccggct cggcgacgcc gagttcgttg
       61 atgcggttgt gcagcgtcgt catgttctgc cggatggcga actcctgaat ccgctttacg
      121 gcctcgggct tcagcgtggc gaccagcttg agatcctcgc cctcaccctg gtccgccagg
      181 agcagatcgg actgattgtc ctcgagtacc atccgtccct tgttgcgtgc ctcgctgtcg
      241 cggaaacgaa cgatcacacg ctcgccttcg cgcgagatgc cggcgtggcg gatggtcttg
      301 tcgcgcagca ggctgcgcag atcgccactg attgaatcca gacgcttggt cagcgcgccc
      361 ttcatgtcga cctgcagcag gaaatgcaca cccccgcgca ggtccagacc gagatacatc
      421 ggcagggcgt gcaggctact cagccactgc ggcgaactcg acaggagatt cagtgcaacc
      481 acatactgcg ggttgctggc atccgggttg aactgtttct ccagcaggtc tttggcctgc
      541 aactggctgt cggtggtgcg cagccgaacc ttgaccccgt tgctgtccag gaagatgccg
      601 ctgtgctcga cgccagcggt ctgcagggta atggcgacgc gttcacgtgt cttgtcgtca
      661 accttgagcg tcgctttggc actggaaacc tgcaccgcag gggactcgcc gaaaaagttc
      721 ggcaaggtgt ataccaggcc gagcagtatg gcgacggcga cgatgacgta tttccagagc
      781 gggtaacgat tcatgggtgc ttgcggtccg agcgcgcggg tactgccgca gggcaggcta
      841 cccgagaggt ggttaaagcg acttcagcgt tcccttgggc aggaccagcg taaccgcctg
      901 cttctggacc tggatttcga ccgtgtcggc gatttcgaga gcgaggaaat tgtcgctgac
      961 ccgggtgacg cggccggcaa tgccgccacc ggtgaccact tcatcaccct tggcaagacc
     1021 ttcgaccagc gccttgtgct ccttggcgcg cttcatttgc ggtcgaacca tcagaaagta
     1081 gagcaccacg aacatcagaa tgatcggcag caattgcatg aggccgcctg tcgggtcggc
     1141 ggcggcagcg gcttgttggg cgtgggcggt gctgatcagc acatcagtct cctaaagcag
     1201 aatgtttgaa ttgaacccga gattgtacca tcccgtcagt tcgaccgagc gcgtgcgctg
     1261 tggaagcttt ccacggatct cgccagtgtg ccagtggaaa tcgcagtgcg caggtcgccc
     1321 atcagttcct gatagtagtg caggttatgc agcgtcgcga gttgagcggc gagaatctcg
     1381 ccgacgcggt tgaggtggtg gagataggcg cgcgaaaagt ttcggcaggt gtagcatgcg
     1441 caactcgcgt caagcgggcc tggatcgtgc ttgtacctgg cgttgcgaat ccggacatcg
     1501 ccgtggcgag tgaatagatg gccattgcgc gcattgcgtg ttggcaacac gcagtcgaac
     1561 atgtcgattc cggcttgcac ggcggccacc agatcttcgg gcgtgccgac ccccatcaga
     1621 tagcgcggtt tgtgttgtgg caggcgtggc gcggtgtgcg cgaggatgcg cgccatgtcg
     1681 gcttttggct cgccgaccga cagtccgccg atggcgaagc cgtcgaagcc gatgtcgacc
     1741 agcgcggcca gcgattcgtc gcggagatct tcgtacatcc cgccctggac gatgccgaac
     1801 agcgcgttgc tgttctccag atggtcgtgt tgcgtccgcg agcggcgcgc ccagcgcagc
     1861 gacaggcgca tcgatgtggc cgcttcgtca tgggtggccg ggtagggcgt gcattcgtca
     1921 aggatcatca caatgtcgga gtcgagcacc ttctggatgt gcatcgattc ctcgggggtc
     1981 aggaacaggc ggtcgccatt gatcggcgac tggaacctga cgccctcctc ggtgatcttg
     2041 cgtagcgcgc ccagtgaaaa gacctggaag ccgccggagt cggtgagcag ggggccgtgc
     2101 cagttcatga aacggtgcag gccggcatgt gcgttgatga cttccagacc cggccgcagc
     2161 cagagatgga aagtgttgcc caggcagatc tgcgcgccgg tctcgatgag atcgcgcggg
     2221 gtcatcgcct tgaccgttcc gtaagtcccg accggcatga aggcgggnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnncgg gccagcggca cccggaatgg cgagcaactc
     2401 acgtagtcga gaccggtgcg atggaagaac tcgaccgaag ccgggtcacc tccgtgctca
     2461 ccacagacgc ccagcttgat cccgggacgt gcagcacgac ctttttcgac ggccatcttg
     2521 accagcaggc caacaccctt ctggtcgatc gactggaacg gatcctgctc gtacatgcca
     2581 tccttgaggt agctgggcag gaacttgccg gcatcgtcgc gcgaaaaacc catggtcatc
     2641 tgggtcaggt cgttggtgcc aaacgagaag aactcggctt gtgggccaat gctgtcggcg
     2701 atcagtgcag cgcgcggcgt ctcgatcatg gtgccgagca ggaagtccag tttctcgcca
     2761 cgctcggcga agaccgcctt ggccgtctgg cggatgatcc tggcctggga gttgaattca
     2821 cggactgtcc cgaccagcgg aatcatgatc tcggccttga cgtcgaaacc cttggccttc
     2881 atgttgaggc ccgcctcaag tatcgcgcgg gtctgcattt cggtgatttc cgggtaggta
     2941 atgccgaggc ggcagccgcg gtggcccatc atcgggttga actcgtgcag atcatcgacc
     3001 cgcatcttga tcacccccag aggcacgttc atttcgtgag ccatgatgcg ctggttttct
     3061 tcgtcgtggg gaacgaactc gtgcagcggc gggtcgagca ggcggatggt caccgccagt
     3121 ccactcatct cgcggaacag accttcgaag tcgccccgct gcatgggcag cagcttggcg
     3181 agcgcgcgcc ggcgcccggc ttcgtcgtcg gcgagaatca tttcgcggac cgccttgatg
     3241 cggtcacctt cgaagaacat gtgttcggta cgacacaggc cgatcccctt ggcgccgaag
     3301 ttgcgtgcgg ccttggcgtc ctcgggggta tcggcgttgg cccgcacacc aagccgtttg
     3361 taacggtcgg cgagttccat cagcgtgccg aagtcaccgg tgagttcagc ttccttggtc
     3421 ggtacctggc ccaggtagac ttccccggtc gatccgtcga gtgacagcca atcgccttca
     3481 ttataggttt cgctgccaat cgacatcgtg cgcgcgtggt agtcgacatg caccgcgccg
     3541 gcaccggaga cacagcattt gcccatgccg cgcgcgacca cggccgcgtg cgaggtcatg
     3601 ccaccgcgag cggtgagaat tcccttggcg acgctcatgc cgcgcaggtc ttcaggcgag
     3661 gtttcctgcc gcacgaggat cacggtcttg ccttttctga cccactcctc ggcttcgtcg
     3721 gcgaagaaga cgatctgtcc ggtcgccgcg ccgggagacg ccggcaggcc atgcgcgatc
     3781 gagcgagcct tcttgattgc cgccggatcg aagaccgggt gcaggagttc gttcaagcgc
     3841 tcggggttga cgcgccgcag ggcttccttt tcatcaatga tgccctggcg cagcatttcc
     3901 atcgcaatgc gcaccattgc cgagccggtg cgtttgccgt tgcgggtctg cagcatccac
     3961 ag
//
LOCUS       DAOSIT010000179         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20130_length_3958_cov_9.623879, whole genome shotgun sequence.
ACCESSION   DAOSIT010000179 DAOSIT010000000
VERSION     DAOSIT010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20130_length_3958_cov_9.623879"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLL14_05590"
     CDS             complement(<1..635)
                     /locus_tag="PLL14_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03429.1"
                     /translation="MHQSSIAIDSKLPRVGTTIFTVMSKLAADCGAINLSQGFPDFQA
                     DPALFDATWRAMRDGRNQYPPMAGVPELRSAIADKVAALYGADYDADSEITVTAGATQ
                     AIFTAIAAFVRTGDEVIVFEPVYDSYVPAIETVGGKAVYAALAFPDYRPDWEQVRALL
                     SPRTRMIIINTPHNPTGSLLEADDLMELAALTRDTDIVVLADEVYEHIVFDA"
     gene            743..1831
                     /locus_tag="PLL14_05595"
     CDS             743..1831
                     /locus_tag="PLL14_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HOG03430.1"
                     /translation="MFSPELHPRMVMCAPGLRSLAAAALLTLGLSCEAAAPALPALGA
                     ELNDLTVSGVSSGAYMAVQFQVAHSRLVRGAGILAGGPYGCAEGSIWRALTRCMSPAS
                     WAPLPSVTELRRGAEALSSAGRIDPVNNLRDDRVWLLSGGRDETVKTPVMDRLAAFYA
                     EWLAPAAIRFVKVPDAGHAMISIADPQANACDSQQTPFINRCGDLDAAGEMFAHLLGP
                     LQPATRAAQGETLIFDQRPFVEGKAIDAGLADDAYVYVPQLCRSTPCRLHVAFHGCRQ
                     SAAQIGRRFVDGAGYNAWADSNRLIVLYPQTMPRYGLTIGSWTWLNNPLGCWDWWGYS
                     GSDYLTRAGPQIKAVRAMIDRLAAPRLP"
     assembly_gap    2108..2207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2208..>3958
                     /locus_tag="PLL14_05600"
     CDS             <2208..>3958
                     /locus_tag="PLL14_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG03431.1"
                     /translation="LEIHIAHINDHHSHLEADPDFEMGIDGITTRVDAGGFPRLTTLF
                     KASEHLPNLLKIHAGDAMTGTLYHTLYQGEADAALMNTVCFDVFEIGNHEFDEGDAGL
                     RGFLDHLRKGKCQTAVLAANVEPAIGSALAPSTTHDYLKPYVLKQFGEVTVAIIGIEV
                     RDKTMHSSRPLRSTLFHDEVDTAQKTIDVLRAQGIRHIVLVTHQGYEADKAMAARLGE
                     VDVIIGGDSHSLLGDFTAIGIRATSGPYPSVVYNRDGERVCIGQAWEYAKAFGLMQIR
                     FDDRGAVLACSGTVTVPIGDDFRRKVGSGDFVAVDEATRKRILESLQEQGAARVVAAD
                     PVAEAELARYAGRLAEMKRQKIGIARESLCLVRVPGESTNRSAGLSGCEDANTLARGS
                     DIAQAVAEAYRQASKLADIALQNGGGIRTALPRGDVTYNTAYTVLPFNNVLFELRLSG
                     RQIVEVLEEAVGNYLDRGSSDGSHPYAAGLRWDLDLSQARGSRFAHIEVMLKGRDTWQ
                     ALAPEQIYTVVTSDFLALGGDGYATLAEVHQTGQSVNTYLNYTQTFVDYLLAQGTVAR
                     PLRANYSHQKVVGRDGKA"
BASE COUNT          682 a         1265 c         1226 g          685 t
ORIGIN      
        1 gcatcgaaga cgatgtgctc ataaacctcg tcggcgagca ccacgatgtc ggtatcgcgc
       61 gtcagcgccg ccaactccat cagatcgtcg gcttcgagca ggctgccggt cgggttgtgt
      121 ggcgtattga tgatgatcat gcgcgtgcgc gggctcagca gcgcgcgcac ctgctcccag
      181 tccggccggt agtcggggaa agcgagcgcg gcatagaccg ctttgccgcc gaccgtttca
      241 attgccggca catagctgtc atagaccggc tcgaatacga tgacctcgtc acccgtgcgt
      301 acaaaggccg caatcgccgt gaaaatggcc tgcgttgcac cggcggtgac ggtaatctcg
      361 ctatcggcgt cgtaatcggc gccgtatagc gccgccactt tgtcggcaat cgccgagcgc
      421 aactcgggaa cgccggccat tggcggatat tgatttctgc cgtcgcgcat cgcccgccag
      481 gtggcatcga acagcgcggg gtccgcctga aaatcaggaa aaccctgcga gagattgatc
      541 gctccgcaat cggccgccag tttggacatc acggtaaaga tggtggtccc tacccgcggc
      601 agcttcgagt cgatggcgat ggacgattgg tgcatggcaa tccgcggctg gcgcggtgga
      661 gaatggtcga gcgcgtagtg tagggttgcc ccggcgggga aacaagcccg aacgtcgtgc
      721 acctgcgagc cgatctgtta gcatgttcag tccagaactt catccccgca tggtcatgtg
      781 tgcccccggt ctacgaagcc ttgccgcagc cgccttgctg acgctcggcc tgtcgtgcga
      841 ggccgccgcc cccgcgctgc cggcgctggg tgccgaactg aacgatctca ccgtctccgg
      901 ggtttcctcg ggtgcctaca tggcggtcca gttccaggtc gctcattccc gactggtacg
      961 cggtgccggc attcttgccg gcggacctta cggctgcgcc gaagggtcga tctggcgagc
     1021 gctcacgcgc tgcatgtcgc cggcgtcctg ggcgccgttg ccgtcagtca cggaacttcg
     1081 gcgcggcgcc gaggcgctgt cgagcgccgg acgcatcgat ccggtcaaca atctgcgcga
     1141 cgaccgcgtc tggttattgt cgggcggcag ggacgagacg gtgaagacgc cggtgatgga
     1201 ccggctggcg gccttctacg cggagtggct tgcgccggcc gccatccggt tcgtcaaggt
     1261 gcctgacgcc ggacacgcga tgatctcgat cgccgatccg caagccaacg cctgcgacag
     1321 ccagcaaacg ccgttcatca accgctgtgg cgacctcgac gcggccggcg aaatgttcgc
     1381 gcacttgctc ggtccgctgc agcccgcgac aagggccgcg cagggcgaaa cgttgatctt
     1441 tgaccagcgg cccttcgtcg aaggcaaggc gatcgacgcc ggcctggccg acgatgctta
     1501 cgtctatgtt ccgcaactgt gccgcagtac gccgtgccgg ctgcatgtcg ccttccacgg
     1561 ctgccgccag agcgcggcgc agatcggcag gcgcttcgtc gatggcgccg gctacaacgc
     1621 ctgggcggac agcaaccggc tgatcgtcct ctacccccag accatgccac gctatggctt
     1681 gacgatcggg tcgtggacct ggctcaacaa tccgttgggc tgctgggact ggtggggcta
     1741 ttcaggcagc gattacctga cgcgcgctgg cccgcagatc aaggccgtgc gcgcgatgat
     1801 cgaccgcctc gcggcgcctc gcctgccgta atatagcgaa ggcagcgccg gtcgccaggc
     1861 accgacactt ccgccacggc gggaacgcgc ctcgctttgc cggcgcgctc gattcggggc
     1921 cgtgatcttg ccccatgcac ggcgaccgcg gttatcctac gggtttgagc catccgggct
     1981 gacttgtctc atcccgccgc cggagccgat cgcatgcgcc aaactcctct ccgcaagtgc
     2041 ctgatgcttg ccgcctccgt cctgctgctc gggctgagcg catgcacgac ccaaacggtc
     2101 gtggattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg gaaatccaca
     2221 ttgcccatat caatgaccac cattcccacc tcgaagccga ccctgatttt gaaatgggca
     2281 tcgacgggat cacgacgcgt gtcgacgccg gaggatttcc gcgcctgacg accctgttca
     2341 aggccagtga gcacctgccg aacctgctca agattcacgc cggagacgcg atgaccggta
     2401 ccctttacca taccctgtat cagggggagg ccgacgcggc gttgatgaac accgtctgct
     2461 tcgacgtttt cgaaatcggc aaccacgagt tcgacgaggg tgatgccggt ctgcggggat
     2521 ttcttgatca tctccggaag ggcaagtgcc agacggccgt tctcgccgcc aacgtcgaac
     2581 cggcaattgg ctccgcgctt gcgcccagca caacgcacga ctatctgaag ccctacgtgc
     2641 tgaaacagtt cggcgaggtg acggtggcca tcatcggcat agaagtgcgc gacaagacga
     2701 tgcattcctc gcggccgctg cgcagcacgc tcttccacga cgaagtcgac accgcgcaga
     2761 agaccatcga cgttctgcgc gcacagggga ttcgccacat cgttctcgtc acccatcagg
     2821 gttacgaggc cgacaaggcg atggccgccc gtctcggcga ggtggacgtg atcatcggcg
     2881 gtgactcgca cagcctgctc ggtgatttca cggccatcgg catcagggcg acgtccgggc
     2941 cgtatcccag cgtcgtctac aatcgcgatg gcgagcgggt gtgcatcgga caggcctggg
     3001 aatacgccaa ggccttcggt ctgatgcaga tccgcttcga cgatcgcggt gcagtcctcg
     3061 cttgcagcgg cacggtcacc gtaccgatcg gcgacgattt ccgccggaag gtcggcagcg
     3121 gcgacttcgt cgcggtcgac gaggcgactc gcaaacggat tctcgaaagc ctgcaggagc
     3181 agggtgccgc ccgggtcgtg gcggcggatc cggtggccga ggccgaactg gcgcgctatg
     3241 ccggtcgcct tgcggaaatg aagcggcaga agatcggcat cgcccgtgag tcgctgtgcc
     3301 tggtacgcgt ccccggtgaa tcgaccaacc gcagcgccgg cctgtccggc tgcgaagacg
     3361 ccaataccct ggcccgcggc agcgatattg cccaggccgt cgccgaagcg taccgccagg
     3421 ccagcaagct cgccgacatc gccttgcaga acggcggtgg aatccgcacc gcgttgccca
     3481 ggggcgacgt cacttacaac accgcctaca ctgtcctgcc gttcaacaac gtgcttttcg
     3541 aactgcggct cagcggtcgt cagattgtcg aggtcctgga ggaggcagtc gggaattacc
     3601 tcgatcgcgg cagttccgac ggatctcacc cctacgcggc cggtttgcgc tgggatctcg
     3661 atctgtcgca ggcgcgcggc agccgcttcg cccatatcga ggtcatgctc aagggccgcg
     3721 acacctggca agccctggcg ccggaacaga tctatacggt ggtgaccagc gacttcctgg
     3781 ccttgggggg cgacggctac gccacccttg ccgaagtcca tcagacaggg cagagcgtga
     3841 atacctatct gaactacacg cagacctttg tggactacct gctggcacag ggtacggtcg
     3901 cccgaccgct acgggccaac tacagtcacc agaaggtcgt cggccgcgac ggaaaggc
//
LOCUS       DAOSIT010000180         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20185_length_3949_cov_7.477658, whole genome shotgun sequence.
ACCESSION   DAOSIT010000180 DAOSIT010000000
VERSION     DAOSIT010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20185_length_3949_cov_7.477658"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLL14_05605"
     CDS             complement(<1..1054)
                     /locus_tag="PLL14_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03432.1"
                     /translation="MENSDDYIYFKDRNHVFTGASQTLVALCDPAEHWSDLLGQTDYD
                     VFPEEFADEYYRLEKQVFAGAPIAIGIQETRSKDGRRGWIDNRKYPITNEKGELVGLY
                     GIARDITERVLAESALRESEERYRSLYDSMTEGVALHELIRDPSGVPIDYTILDVNPA
                     FERHTGLSRAAILGKSATLAYQTPVAPFLEIYSVVATTRQATVFEQYFEPLQKHFRIT
                     VFSPRRQQFVTVFQDITESKRLEQGLRESEMRFRSLFENTPTIAVQGYDRSRRVIFWN
                     LASERVYGFSREEAIGRRLEELIIPEGMRAEVIRAVDAWVDGGPAIAAGELLLQRKDG
                     SPVAVYSSHAMQRGSAG"
     assembly_gap    1067..1166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..>2626)
                     /locus_tag="PLL14_05610"
     CDS             complement(<1167..>2626)
                     /locus_tag="PLL14_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOG03433.1"
                     /translation="GMAYAMLAGLPPVNGLYASILPAIAYALFGSSMVQSVGPMAITS
                     LMTGTALAALAPPGSALSVVLAGQLALIAGVVLFLCGMLRLGFLAGFLSRPVMSGFIT
                     GASLLITGGQIEPLLGGPLAASHLPSAIVGLSSLLALWLAQKFLVRGLGVLGLPRRVA
                     EMLSRLAPVLVLVVATAAVGVLDLAQFGVKVVGEVPSGMPAIQLMLSADHWRSLLVPA
                     LLVAFMIFLSSQSAAQSLALKRGERILTNHELLGLGAANLASAVSGGFAVTGSISRSA
                     VNYSAGANTPLASVISAGLIVVVLWVPTTWVSLLPLPALAATIILPVLGMIDLTTLRD
                     AWRYDRSDAGALLATSAGVVLLGVEEGVILGVVLSLATLIWRSSRPHIAVIGRIPGSE
                     HFRNVERHQVDTLPEVLMLRIDADLYFGNVGVVFDHLESLLKARAAQGLRTRHVVLVM
                     SAVSLIDTTGLYALIEINRSLQAQRIKLHLSEVKGPV"
     assembly_gap    2627..2726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2727..2935
                     /locus_tag="PLL14_05615"
     CDS             <2727..2935
                     /locus_tag="PLL14_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03434.1"
                     /translation="LRPFEAFPIRRQRCLQLAIENTSDISLVLAHPPGQRFNEMEAHG
                     IQSIRTPHAQRSDLQTIINNLIDR"
     gene            complement(2961..3605)
                     /locus_tag="PLL14_05620"
     CDS             complement(2961..3605)
                     /locus_tag="PLL14_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG03435.1"
                     /translation="MDDSDAGIEQTASVWAVFGDLMSGLLGACVLILVGVLGIQLELA
                     THLEAEVQKRQMEERRRMALEKALAIPLAAGRVTLNNGRIGISGSVLFSLNSAQLQPD
                     GRLLLKSLVAPLRVYLGERDELLMVSGFTDDEPIQSGNNRFADNWELSAQRALTVTRA
                     LIDEGIPSSLVFAAAFGMEQPVASNADDRGRSRNRRVEMAPVPKSANATMPAHD"
     gene            complement(3605..>3949)
                     /locus_tag="PLL14_05625"
     CDS             complement(3605..>3949)
                     /locus_tag="PLL14_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03436.1"
                     /translation="NAGSQFAEQVSAESARLSDIAAQLAGSAIEVSSLSEAFGFAVQL
                     FSDANEKLIDNLQSIEASMDKSTARSDEQLAYYVAQAREVIDLSILSQKDVVEALRQL
                     LDKQAATADQVS"
BASE COUNT          784 a         1208 c         1064 g          693 t
ORIGIN      
        1 ggccggcact gccgcgctgc atggcgtgac tggaatacac ggcgacgggc gagccatcct
       61 tgcgctggag cagcagttcg cccgcagcga tggcagggcc accatcgacc caggcatcga
      121 cggcgcggat cacctccgct cgcatgcctt ccggaatgat cagttcttcg aggcggcggc
      181 cgatggcttc ttccctcgaa aatccataga cccgctcact cgccaggttc cagaagatca
      241 cgcggcggga acggtcatac ccttgcacgg cgatcgtggg ggtattttcg aagagcgaac
      301 gaaagcgcat ctcgctttct cgcagcccct gttccagacg cttgctctcg gtgatgtcct
      361 gaaagacggt cacgaactgt tgtcgtctgg gcgaaaaaac ggtgattctg aaatgtttct
      421 gcaggggttc gaagtactgc tcaaagaccg ttgcttgccg agtagtagcg acaaccgagt
      481 agatttcgag aaacggggcg actggcgtct ggtaggccaa cgttgctgat ttccccagga
      541 tcgccgccct ggacaaaccg gtgtgtctct cgaaggccgg gttaacgtcc aggattgtgt
      601 agtctatggg aactccggag ggatcccgta tcagttcgtg cagggcgacc ccttcggtca
      661 tcgagtcata cagcgatcga tatctttctt cgctttccct cagcgcgctc tctgccagga
      721 ctcgctcggt aatatcgcga gcgattccgt acaggccgac cagttcaccc ttttcattcg
      781 tgatcggata cttgcgatta tcgatccagc ctcgccggcc atccttgctc cgggtttcct
      841 ggatgccaat ggctatgggt gctccggcaa agacctgctt ttccagacgg taatactcgt
      901 ctgcgaactc ctccggaaaa acatcatagt ctgtttgacc cagcaggtcg gaccaatgct
      961 cggccggatc acacagggcc accagggtct ggctcgcccc ggtaaagacg tggttgcgat
     1021 ccttgaagta gatgtagtca tccgagttct ccatcatggc tcggaannnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnaccg gccccttgac ctcgctcaga tgcagcttga
     1201 tccgctgtgc ctgcaggctg cggttgatct cgatcaaggc atagagtccg gtggtgtcga
     1261 tgaggctcac cgccgacatg accaggacca catggcgtgt gcgcagaccc tgggccgccc
     1321 gtgccttcag caggctctcg aggtggtcga agacgacacc gacgttgccg aaatacaggt
     1381 cggcgtcaat acgcagcatc agcacttcgg gcagggtgtc cacctgatgc cgctcgacat
     1441 tgcggaaatg ctcgctgccg gggatccggc cgatgacggc gatatgcggc cggctggaac
     1501 gccagatcag agtggcgagc gacagcacga caccgagaat cacgccctcc tcgacgccaa
     1561 gcagcaccac gccggcgctg gtggccagca gcgcgccggc atcgctgcga tcgtagcgcc
     1621 aggcgtcacg taacgtcgtc aggtcgatca tgccgagcac cgggagaatg atcgtcgccg
     1681 ccagcgccgg caatggcagc agcgacaccc aggtggtcgg tacccacagc acaaccacga
     1741 tcagaccggc cgaaatcacg ctggccaacg gtgtgttggc gcctgccgag taattcaccg
     1801 ccgaccgcga aatgctgccc gtcaccgcaa agccgcccga cacggcgctg gccagattgg
     1861 cggcgccgag ccccagcaac tcgtggttgg tcaggatcct ttcgccgcgc ttgagcgcca
     1921 gcgattgcgc cgctgactga cttgagagaa agatcatgaa agcgaccagc aaggccggca
     1981 ccagcaagga ccgccaatgg tcggccgaca gcatcagttg gattgcgggc atgcccgatg
     2041 gaacctcgcc caccaccttc accccgaact gcgccagatc cagcacgccc accgcagccg
     2101 ttgcgacgac caacaccagc accggcgcca acctggaaag catctccgcc actcttctgg
     2161 gcagccccag gacacccagc ccgcgtacca gaaatttttg cgccagccac aaggccagca
     2221 gcgaactcag gccgacgatc gcgctgggca gatgactggc tgccaacgga ccgccgagga
     2281 gcggttcgat ttgaccgccg gtgatcaaca gggaagcccc ggtgataaat ccgctcatca
     2341 ccggccgact gaggaaacca gcgagaaagc cgagcctgag catcccgcat agaaacagca
     2401 ccacgccggc aatcagcgcc aactgaccgg ccagaaccac actcagggcc gacccgggtg
     2461 gcgccagggc ggccagcgcc gtgcccgtca tcagcgaggt gatggccatc ggccccacgg
     2521 attgaaccat gctgctgccg aacaacgcat aggcgatggc tgggaggata ctggcataca
     2581 gcccgttcac cggcggcagg ccggcgagca tggcataggc catgccnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnntcct gcgcccgttc gaggcgttcc ccatacgccg
     2761 ccagcgctgc cttcagcttg ccatcgagaa tacgtctgac atctccctgg tgctcgcgca
     2821 cccgcctggc cagcgcttca atgaaatgga agcgcacggg atccagtcga tccgcacccc
     2881 gcatgcgcaa cgaagcgatc tccagaccat catcaataac ctgatcgaca gatgatgctg
     2941 ggagtccgtt gcgacgggat tcaatcatgg gccggcattg ttgcattcgc ggatttcggt
     3001 actggtgcca tttcaactcg ccgattccga gatcgtcccc tatcatcggc attggatgcc
     3061 accggctgct ccataccgaa agccgcggcg aaaaccagag atgacggtat gccctcgtcg
     3121 atcagcgcac gggtaaccgt caacgcccgt tgggcagata attcccaatt gtcggcgaag
     3181 cgattattgc ctgactgaat cggttcatcg tccgtaaacc cgctgaccat caacaattca
     3241 tcacgctcgc caagatagac gcgcagcggc gctaccagac tcttcaggag cagccgacca
     3301 tcgggttgca gttgcgccga gttcaacgaa aacaatacgc tgccgctgat gccgatgcgc
     3361 ccgttgttca aggtgacccg ccctgccgcc agcggaatgg ccagggcttt ttccagtgcc
     3421 atgcggcggc gctcttccat ctgccgcttt tgcacttcgg cttccagatg ggtcgccaac
     3481 tccagttgga tgcccaggac gccaaccaga atcagcacgc aagcgcccaa caagcccgac
     3541 atcagatcgc caaagacggc ccataccgac gcggtctgct caatgccggc gtcactgtca
     3601 tccattagct cacctgatca gcagtcgccg cctgtttgtc cagaagttga cgcagcgcct
     3661 cgacgacgtc cttttgcgaa aggatactca gatcaatgac ttcgcgagcc tgcgccacgt
     3721 aataggcaag ttgctcgtca cttcttgcgg tcgatttgtc catcgaggct tcaatgcttt
     3781 gcagattatc gatcagtttc tcgttggcat cactgaacaa ctgcacggca aaaccgaatg
     3841 cttcgctcag gctcgatacc tcgatggcac tgccggcaag ctgcgctgca atgtcggaaa
     3901 gcctggccga ttctgcgctg acctgctcgg caaactggct gccggcatt
//
LOCUS       DAOSIT010000181         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20220_length_3945_cov_9.021594, whole genome shotgun sequence.
ACCESSION   DAOSIT010000181 DAOSIT010000000
VERSION     DAOSIT010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20220_length_3945_cov_9.021594"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..2069)
                     /locus_tag="PLL14_05630"
     CDS             complement(102..2069)
                     /locus_tag="PLL14_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG03437.1"
                     /translation="MTANPGTADPGDRLAGEARYRRFLDALLVGDVAYCRAEVQAWLD
                     AGRGVRTLYEDLIQRALYDVGMLWESGRASVADEHLATAISESLLNLSYPYLFDRPST
                     GCSALVTCAANEHHQIGARMVADIFELHGWRSYFLGANTPQGDILALMASRRPQVVSL
                     SLTVHCHLERLLDIVGAIRGLYPEMPIIVGGQGLGDIGRARVEVFPGVRVIGSLAELE
                     AWIEDNAASPGTTPAVAAAVTPLPAAQPSEVVESSTAAVDKTLDNTSASTSVIAPDPL
                     RLAAVEACVRDEAPLLALTLDSELRVIALNAFAAQLLPPARLGRPLAALLLDFASRNI
                     DVPALVAAGRSAGGGRSRLTLATAAGVPETLTFSFHPLAAGTLALGWPDFAEQRRLRG
                     EVLNLNRELNDLIRQLHQSNAELRELNTLRETFLGMAVDELRTPIDLIMTHTELVLDA
                     TGTTPVSEHHASLRSCLAAAAGMKRRVNDFLAAATPAAGRLRLELAPASVADLVVATL
                     PLLQRLAQPRNVRLFADTAAAPGEWLLDAGRLQQVLVHLAGSAIKNSASGQQVRIEAW
                     IDAELLHVAVHDQGPDIAVADQLRWFDGVEHGATRPGDSTRSVGPGLAIARRIIEAHG
                     GWIGVDSAPGRGACFTFSVPACRADATEEQA"
     assembly_gap    2234..2333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2334..>3945)
                     /locus_tag="PLL14_05635"
     CDS             complement(<2334..>3945)
                     /locus_tag="PLL14_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03438.1"
                     /translation="DLFWISLSVASALFAVVFFGVMLRQQQRLRLSESLRQAQAEALR
                     MQQFVDRQREERGALVSHYETMVGKARDIILLLDDAGRIVEANEAAVAAYGWSIDELR
                     TLHIRDLRCEDTPALFTEQWQASANPEGALFEALHRRRDGRSFPVEISSRAIEIEGKP
                     YRQSFIRDISERRKAASALQRSHRALRTLSECNQAMIRADAEPALLAEICQLVVHFGG
                     YRMAWVGYADDEPLHSVRPVAQAGFEDGDLATADMTRDDVEQGRGPSGTAIRERRPVL
                     AQNLVDDPHFGPWRDAALRRGYAASIALPLLAADSDCLGALNLYAGTADAFDADETRL
                     LVELAGDLAYGIRTLRDRTARERAEADLRATTQQLQHLLEASPTVLYALRVADGVTRT
                     VQVSANIERISGHTIAEALQDGWWAAGLHPDDRQAALTAGLLPGAAATVHEYRFAHKD
                     GHYLWIRDEQRLIRDASGQAAEIVGAWTDITTARLATLALLDSERRYREMFAENPLPM
                     WVFDHATLAFIEVNDAAIRHYGYTREEFL"
BASE COUNT          598 a         1234 c         1359 g          654 t
ORIGIN      
        1 cagcgtgcgg caggtgactt cgcgctgtgc cgggtcgtcg taggcgacga gaatacgcgc
       61 aggtgctgtc gccggcgtcc gctcggtcac cgcgccagtg ctcatgcctg ctcctctgtg
      121 gcgtcggcgc ggcacgccgg aacgctgaag gtgaaacagg cgccacggcc cggtgcgctg
      181 tcgacgccga tccagccgcc gtgcgcctcg atgatccggc gggcgatggc cagtcctggg
      241 ccgacgctgc gggtgctgtc gccggggcgc gtggctccgt gctcgacgcc gtcgaaccag
      301 cgcaactgat cggcgacggc aatatccgga ccctgatcgt gcaccgcgac atgcagtagt
      361 tcggcgtcga tccaggcctc gatgcgcact tgctggccgc tggcggaatt cttgatggcg
      421 ctgccggcga ggtgcaccag cacctgctgc aaccggccgg cgtcgagcag ccactcgccg
      481 ggtgccgcag cggtgtcggc gaagagccgc acattcctgg gctgcgccag tcgctgcagc
      541 agtggcagcg tcgccacgac aaggtcggcg acactggccg gcgccagttc cagccgcaga
      601 cggccggctg cgggcgtcgc cgcggcaagg aaatcgttga cacgccgttt catgccggcg
      661 gcggcggcca ggcaactgcg caacgaagcg tggtgttcgg agacaggcgt cgtgccagtg
      721 gcgtcgagca ccagttcggt gtgagtcatg atcaggtcga tcggtgtgcg cagttcgtcc
      781 accgccatgc cgagaaaggt ttcccggagc gtgttgagtt cacgcagttc ggcgttgctc
      841 tgatgcagtt gccggatcag atcgttaagt tcgcggttga ggttgaggac ctcgccgcgc
      901 agtcgccgct gctctgcaaa atcgggccag ccgagagcca gtgtgccggc ggcaagcggg
      961 tggaagctga aggtcagggt ttccggtacg ccggcggccg tcgccagggt cagccgggac
     1021 ctgccgccac cagcgctcct gccggcggcg accagtgctg gcacgtcgat gttgcggctg
     1081 gcgaaatcga gcagcaacgc ggccagcggt cgcccgaggc gtgccggtgg cagcagttgt
     1141 gcggcgaacg cgttgagcgc gatcacccgc agctcgctgt cgagtgtcag tgccaacagc
     1201 ggcgcctcgt cgcggacgca agcctcgacc gccgccaggc gcaatggatc cggcgcgatc
     1261 acggacgtgc tggcgctggt gttgtccagg gtcttgtcga cagcggcggt gctgctttcc
     1321 accacttcgc tcggctgcgc tgcggggagc ggggtgactg cggcagcaac ggccggggtg
     1381 gtgccgggac ttgctgcgtt gtcctcgatc catgcttcga gttcggccag cgagccgatg
     1441 acgcgcacac caggaaacac ttcgacgcgt gcccgaccaa tgtccccgag accctggccg
     1501 ccgacaatga tgggcatctc cgggtacagc ccgcggatcg cgccgacgat atcgagcaac
     1561 cgttcgaggt ggcagtggac ggtcagtgac aggctgacca cctgcggtcg ccggctcgcc
     1621 atcagcgcca gaatgtcacc ctgcggcgtg ttggcgccga gaaaatagct gcgccagccg
     1681 tgcagctcga agatgtcggc gaccattctg gcgccgatct ggtgatgttc gttggccgcg
     1741 caggtgacca atgccgagca gccggtggac ggtcgatcga agaggtaggg gtagctcagg
     1801 ttgagcagac tctcggaaat cgccgtggcc aggtgttcgt cggccaccga ggcgcgaccg
     1861 ctctcccaga gcatgcccac atcgtacagt gcgcgctgaa tgaggtcctc gtagagcgtg
     1921 cggactcccc ggccggcgtc gagccaggcc tggacctcgg cgcggcagta ggcgacatcg
     1981 ccgaccagga gagcgtccag aaagcgtcgg tagcgggcct cgccggcgag tctgtcgcca
     2041 gggtcggcgg tgccgggatt cgccgtcatg cgcatcgcct aagccggatc gcgcttgacg
     2101 gcgccataac gtggtcgccg gccggcttcg gcgagcaggg cattgacctc gcgcttgagc
     2161 tgcagcaccc ggccttcgcg gttccgggtc accgcgtgcc aacgacgcag ttcttccagc
     2221 tgggcgagca ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaggaa
     2341 ttcctcgcgc gtataaccgt agtggcggat ggcggcgtcg ttgacctcga tgaaggccag
     2401 cgtcgcgtgg tcaaaaaccc acatcggcag tggattttcg gcaaacattt cgcggtagcg
     2461 tctttcgctg tccagcagcg ccagcgtggc cagcctggcg gtggtaatgt cggtccacgc
     2521 gccgacgatt tcggcggctt gcccgctggc gtcgcggatc agccgctgct cgtcgcggat
     2581 ccacaggtag tggccgtcct tgtgggcgaa gcgatactca tgaacggtcg cggcagcgcc
     2641 cggcagcagc ccggcggtca gggccgcctg gcgatcgtcg gggtgcaggc cggcagccca
     2701 ccagccgtcc tgcagcgcct cggcgatggt atgcccggag atcctttcga tgttcgcgct
     2761 gacctgcacc gttcgcgtga cgccgtcggc aacgcgcaac gcgtagagca cggtcgggct
     2821 cgcttcgagc aaatgctgca gttgctgtgt ggtggcgcgc agatccgctt cggcgcgctc
     2881 gcgtgcggtg cggtcgcgca gggtgcggat accgtaggcc aggtcgccgg ccagttcgac
     2941 caggagtcga gtctcgtcgg cgtcaaaggc atcggccgta ccggcataaa ggttcagcgc
     3001 tcctagacaa tcactgtctg cggcgagcag cggcagcgcg atcgaggccg cgtagcctcg
     3061 ccgtagcgcg gcgtcgcgcc aggggccgaa gtgtgggtcg tcgaccagat tctgcgccag
     3121 caccggtcgc cgctcgcgga tcgccgtgcc actcgggcca cgcccctgct cgacatcatc
     3181 cctggtcatg tcggcggttg ccaggtcgcc gtcttcgaaa ccggcctgcg cgaccggccg
     3241 cacgctgtgc agcggctcgt cgtcggcata gccgacccag gccatgcggt agccgccgaa
     3301 gtgtacgacc agttggcaga tctcggcgag cagtgccggt tcggcgtcgg cgcgaatcat
     3361 ggcctggttg cattcgctca aggtgcgcag ggcgcgatgg cttcgctgca gggccgacgc
     3421 tgccttccgg cgttcgctga tgtcgcggat gaagctctgg cgatagggct tgccttcgat
     3481 ctcgatcgcg cgcgaactga tctcgaccgg aaaggatctg ccgtcgcggc ggcggtgcag
     3541 ggcctcgaag agtgcgcctt ccgggttggc ggaagcctgc cattgttcgg tgaacagggc
     3601 cggcgtgtct tcgcagcgca ggtcgcggat gtgcagggtg cgcagttcgt cgatgctcca
     3661 gccataggcg gcgactgccg cttcgttggc ctcgacgata cgaccggcat cgtcgagcag
     3721 gaggatgatg tcgcgcgcct tgccgaccat cgtttcgtag tggctgacca gcgcaccgcg
     3781 ctcctcacgc tgtcgatcga cgaactgctg catgcgcagc gcttcggcct gcgcttgtct
     3841 aagcgattcg gacaaacgca aacgctgctg ctgacgcagc atgaccccga agaatacgac
     3901 cgcgaacagg gccgatgcga cgctgagcga gatccagaag agatc
//
LOCUS       DAOSIT010000182         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20253_length_3941_cov_5.654915, whole genome shotgun sequence.
ACCESSION   DAOSIT010000182 DAOSIT010000000
VERSION     DAOSIT010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20253_length_3941_cov_5.654915"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLL14_05640"
     CDS             complement(<1..922)
                     /locus_tag="PLL14_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOG03439.1"
                     /translation="MDPALWIELVVFVVLLGFSGFFSSTETSLFSLSSFQLDQMRAAR
                     NPRIELIDRLRSEPRRLIVTILIGNEFVNVSASVISAAMIIHLFGAENEMVNLLVMVP
                     ILLLFGEITPKVLAIRNNVAFANVECRPIELVARLITPLREVIRYVSDFFITLIVGKQ
                     RSQGNLVTEDMIRTLVHDAVGEGALDSQEAQYIEKIFDFGNKSLRDIMRPRSDIQFLA
                     SDLPVAELLAQIKASRQSRYPVFKGHRDTILGILHARDLLGVDLARLERDPQGFRKLL
                     RQPSFFPESKPAVELFHTFRQRKLSFALIVD"
     gene            complement(1169..2779)
                     /locus_tag="PLL14_05645"
     CDS             complement(1169..2779)
                     /locus_tag="PLL14_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HOG03440.1"
                     /translation="MPVIELTLLLLLAIAVGGALVRWTPLPLPILLVVVGLAASFLPG
                     LDAIEVDPDLFLLLFIPPILFADAWVLPRRDFVRVLKPVLLLALGLVVLTVVVVGYVM
                     HWLIPAMPLAVAFTLGAIVSPTDAVATVATTEALPMPGRIVHIVNAESLLNDASGLVA
                     FKLAVAAAATGLFSSAEVAGNFVILAGGGMVVGIACEWVGRQLRQRLIRLQAGDPVLQ
                     TLLTVLIPYGAYLAAEALQVSGILAVVAAGLWASGQEVKGLTADARRHAREVWRMLSY
                     ILNGLVFVLLGLQLRRMLTSVDHFAPLDLALYAFVLWVLLMVLRIGWVWASAHVRFRL
                     HWGWAGSRSGPDPKRMLLVSWAAVRGSITLATALSVPAFVAGGMPFPERDLVVFLAAA
                     TILLTLALNGVPLPWIIGKLALDHEEDAAREESTARAEIARAAIGAVALTARNLSDPE
                     DVSFAAQLAHRYEGKLELLEGDAENASLRATHIALRRHLRLAAIHAERARLHELHAAD
                     AINDETLRTIEAELDERELISSTHVNRG"
     gene            complement(2929..>3941)
                     /locus_tag="PLL14_05650"
     CDS             complement(2929..>3941)
                     /locus_tag="PLL14_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOG03441.1"
                     /translation="GTCATALLAVIGKPREAVRAALVHLGFNVCGVLIWLPFIGFIAD
                     LVTAISPSYPNLSGVDRLAAETPRQIANAHTFFNIANTLIFIWFTSQIARLVEWLVPD
                     KPLADEALIVRPRFLQEELLSAPSLALDQVRMEIMHMGETVNAMLQGIMPAIIGGDRK
                     VLEEIRQMDDTVDILHAGIIDYLAKISKQPLGDEQTRELMQLMEAASNLENIGDIVET
                     NLVVLGHDRIRDRIQVGESTQQVLNGFQEAITKAVAAAVQAVGQRNERAGQVVIGMKN
                     EINRIADSAAAHEARRLVAEEPNRIAAYTMEIEIIEKQKRIYYFAKRMAKTVMPAVLQ
                     GQ"
BASE COUNT          794 a         1241 c         1225 g          681 t
ORIGIN      
        1 cgtcgacgat cagcgcgaag gacagcttgc gctggcgaaa ggtgtggaac agttctaccg
       61 ccggcttcga ctcgggaaag aagctcggct gccgcagcag tttgcgaaac ccctgcggat
      121 cacgctccag ccgcgccaga tcgacgccga gcagatcgcg tgcgtgcagg attcccagga
      181 tcgtgtcgcg atgtcccttg aataccggat aacgtgactg gcggctggcc ttgatctggg
      241 ccagtaattc cgccaccggc aagtcggatg ccagaaactg gatgtcggag cgcggccgca
      301 tgatgtcccg caaggacttg ttgccgaagt cgaagatctt ttcgatgtat tgggcttcct
      361 ggctgtcgag cgcgccttcg ccaacggcat cgtgtaccag cgtgcggatc atgtcttcgg
      421 tcaccaggtt gccctgcgag cgctgcttgc cgacgatcag ggtaatgaag aaatcggaga
      481 cgtaccgaat cacttcgcgc aagggcgtga tcaggcgggc gacgagttca atggggcggc
      541 actcgacgtt cgcaaatgcg acattgttgc gaatcgccag gactttcggg gtgatctcgc
      601 cgaacagcaa caggatcggc accatcacca gcaggttgac catttcgttt tctgcgccga
      661 acagatggat gatcatcgcc gccgagatca ccgacgccga gacattgacg aactcgttgc
      721 cgatgaggat ggtgacgatc aggcgccgcg gttcgctgcg cagccgatcg atcaactcga
      781 tacgcggatt cctggctgcg cgcatctgat cgagctggaa cgaactcaag gagaatagcg
      841 aagtttcggt actcgaaaaa aagcccgaaa acccgagcag aacaacaaag acgacgagtt
      901 ctatccagag agcgggatcc atgggtgaca tcctgagaga agtggctgcc aaaagtcatg
      961 cgagcagcgg cctcgtcgcc agcgacgcgg cagtgtcgaa ttcaacggcg attccctgct
     1021 gcacagaccg gtcagccgat tttcaccgag ttcaatgccg tggtcaatta tccctgcccg
     1081 gagtaccaac agtcagcggc agcccaatgc ggtcatcgcg gtctcaaacc ttgcgccggc
     1141 tccggcgtat gctggtgatc gctcagtgct agccgcggtt cacatgcgtc gaggaaatca
     1201 actcgcgctc gtcgagttcg gcctcgatgg tccgcagcgt ttcgtcgttg atcgcgtcgg
     1261 cagcgtgcaa ctcgtgcagg cgcgcacgct cggcgtggat cgccgccaga cgcaagtgcc
     1321 gtcgcagtgc gatgtgcgtt gctcgcaggg aagcattttc ggcatcacct tcgagcagtt
     1381 cgagcttccc ctcgtagcgg tgcgccagtt gtgccgcgaa agacacgtcc tcggggtccg
     1441 aaaggttcct ggccgtgagc gccacggcgc cgattgccgc ccgtgcgatt tcggcgcggg
     1501 cggtgctttc ctcacgggcg gcatcctcct cgtggtcgag cgccagtttg ccgatgatcc
     1561 aggggagggg cacgccattc agggccagcg tcaggaggat cgtcgccgcg gccagaaaaa
     1621 cgacgaggtc gcgctcggga aacggcattc caccggcgac gaaggcggga acggacagcg
     1681 ccgtcgccag cgtgatcgac ccgcgtaccg ccgcccaact caccagcagc atccgtttcg
     1741 ggtccggccc cgagcggctg ccggcccagc cccagtgcag gcgaaatcgt acatgcgcgg
     1801 aggcccacac ccagccgatg cgcaacacca tcaacagaac ccacaaaacg aacgcataaa
     1861 gagccaggtc gagcggggca aagtggtcaa cactggtcag catgcggcgc agttgcaggc
     1921 cgagcagcac aaagaccagg ccgttcagga tgtacgacag catccgccag acctcgcgcg
     1981 cgtgccggcg tgcatcggcg gtgagtccct tgacttcctg gccgctggcc catagcccgg
     2041 cggcgacgac ggccaggatt cccgagacct gcagcgcctc ggcggcgagg taagcgccat
     2101 agggaatcag gaccgtcagc agcgtctgca gcaccgggtc gccggcctgc agacgaatga
     2161 gccgttgccg cagttgacgt ccgacccact cgcaagcgat gccgacgacc atgccgccgc
     2221 cggccagaat cacgaaattg ccggcaactt cggccgacga gaacagcccg gtcgccgccg
     2281 ccgccacggc gagcttgaag gccaccagcc cggaggcatc gttgagcagg ctttcggcat
     2341 tgacgatatg tacgatccgc cccggcatcg gcagggcttc ggtggtcgct acggtggcca
     2401 cggcatcggt cggcgagacg atcgcgccga gggtaaaggc aaccgccagt ggcattgccg
     2461 gaatcagcca gtgcatcaca tagccgacca cgaccacggt cagcacgacc aggcccagtg
     2521 ccagcagcag caccggtttc agcacgcgca caaaatcgcg acgcggcaac acccaggcgt
     2581 cggcgaaaag aatcggcggg atgaacagca gcaggaacag atccggatcg acctcgatgg
     2641 cgtcgaggcc gggcaggaac gacgcagcca ggccgacgac gaccagcagg atcggcaagg
     2701 gcagcggcgt ccagcgaacg agcgcgccgc cgacagcgat cgccagcaac agtaacaggg
     2761 taagttcgat gacgggcatg acggattcta ccgtacgccc gcaaggaatg cgacgagtgc
     2821 cgaacgctgg ggtgtgggta gaggaatagc gcgctcaccc tgccgctagc tgctgcgcat
     2881 tcctcattct ccagccaggc aggtcggtgc agactgtcgc cgcgacggtt actggccctg
     2941 caatacggcc ggcatcaccg tcttcgccat ccgcttggcg aaataataga tgcgcttctg
     3001 cttctcgatg atctcgatct ccatggtgta ggccgcgatg cggttcggct cttcggcgac
     3061 cagccgacgt gcctcatgcg cggctgccga gtcggcgata cggttgatct cgttcttcat
     3121 cccgatcacc acctgcccgg ctcgctcgtt gcgctgtccc accgcctgca cagcggccgc
     3181 aaccgccttc gtaattgcct cctggaagcc gttgagcacc tgttgagtag actcgccgac
     3241 ctgaatcctg tcacgtatgc ggtcatgccc cagcaccacc agattggttt cgacgatgtc
     3301 gccgatgttt tcgaggttgc tggcagcttc catcaactgc atcaactccc tcgtctgttc
     3361 gtcccccagt ggctgcttgc tgatcttggc cagatagtca atgatcccgg catgcaggat
     3421 atcgaccgtg tcgtccattt gccggatctc ttcgagcacc tttcggtcac cgccgatgat
     3481 cgccggcatg atgccctgca acatcgcatt gaccgtctcg cccatgtgca tgatctccat
     3541 gcggacctgg tcgagggcca gcgacggcgc gctcagcagt tcctcctgca ggaacctggg
     3601 acgcacgatc aacgcttcat ctgcgagcgg tttatcgggc accagccatt cgaccaggcg
     3661 tgcgatctgg gaggtaaacc agatgaagat cagcgtgttg gcgatattga agaaagtgtg
     3721 cgcattggcg atctggcgtg gcgtctcggc ggccagtcgg tcgacgccac tgaggttcgg
     3781 gtagctcggc gaaatggcgg tgaccagatc ggcgatgaag ccgatgaacg gcagccagat
     3841 cagcaccccg cagacattga agcccagatg gaccagcgct gcacgtaccg cttcgcgcgg
     3901 cttgccgatc accgccagca gcgccgtcgc gcaggtgccg a
//
LOCUS       DAOSIT010000183         3908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20447_length_3908_cov_11.296392, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000183 DAOSIT010000000
VERSION     DAOSIT010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20447_length_3908_cov_11.296392"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLL14_05655"
     CDS             <1..384
                     /locus_tag="PLL14_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOG03442.1"
                     /translation="DIGLIEGGICNAENIHVLQEFRRQCKVLIAIGACAVNGGLPAQR
                     NHLSLGQCLQEVYLTWPGIVSGQIPNDPELPLPLDKVHPIHEIVRIDYFIPGCPPSGD
                     AIWKFLTDLLDGRTPRLGHPLLHFD"
     gene            396..1874
                     /locus_tag="PLL14_05660"
     CDS             396..1874
                     /locus_tag="PLL14_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOG03443.1"
                     /translation="MASEAFALETAAAEAGTLRRVAIDPVSRVEGHGKVTLLLDANDE
                     VQQVRLHIVEFRGFEKFIQGRPYWEVPVMVQRLCGICPVSHHLAAAKALDHVVGAEKL
                     PASAEKLRRLMHYGQILQSHALHFFHLSSPDLLFGFASDAGRRNIVGVAAAYPEVAKQ
                     GVLLRKFGQEVIRLTAGKRVHGTGAIPGGVNRALSPAERDGLLADIDQIVDWSRAAVQ
                     IVKRLHAADPPLYDSFGTFRSNILSLVGADGALDFYDGRLRARDADGKILVDGFAVEN
                     YQQLIHEEVKPWSYMKFPFLAALGPRDGWYKVGPLARVQNCDSIATPLAEGERREFID
                     HGQGRLIHAPLAYHWARMIEMLHAAETIKDLLHDDDLSGSELSCSGTRRHEGVGVIEA
                     PRGTLIHHYRVDDDDLVTMCNLIVSTTHNNQPMNEAIRRVAQRYLNGRQLSEGLLNHI
                     EVAIRAFDPCLSCATHALGKMPLQLELQDAAGQVIARMRSAS"
     gene            1894..2427
                     /locus_tag="PLL14_05665"
     CDS             1894..2427
                     /locus_tag="PLL14_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOG03444.1"
                     /translation="MSLAPVVILACGNPSRGDDALGPMLLDRLQDWLAGEGLSTGFEL
                     IGDFQWQVEHALDLVGRRLALFLDAGQRTPAPWVFRQVQALATPTPSSHALSPEAVLA
                     VLARIGQPAPPTFVLGVAGERFELGEGLSAAASRHAEGAFALLQVLCRTPRVDAWQAL
                     ATGRPGGIVRWPARDAE"
     gene            complement(2498..2959)
                     /locus_tag="PLL14_05670"
     CDS             complement(2498..2959)
                     /locus_tag="PLL14_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HOG03445.1"
                     /translation="MPRSLTEHFTLDELVASQTATRSGIDNTPGAAALRNLKSLAEVL
                     EEVRTLLGGVSLLISSGYRSPALNAAVGGSAKSAHLLGLAADFTAPKFGTVLQTAKKI
                     AASGIAYDQLIHEYGAWVHLGLAAPGMVPRRQNLSIFQGTGYLPGIIAKPG"
     gene            complement(3020..3847)
                     /locus_tag="PLL14_05675"
     CDS             complement(3020..3847)
                     /locus_tag="PLL14_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03446.1"
                     /translation="MPKKLALLTLHGMGDTSPTYYVELHDLLKKALGNSTWNRIAFRP
                     IYYQNVLQGNQAEIFGRMRDQIDWMKLRRFLLYGFSDAASLEYRKDVAGSPYFETQKI
                     ILQTLDSVFEEAGRSAVPVIVVAQSLGCQVISSYIWDAQQPRPSAGIWRAPPGDGVAA
                     GSTQDQFRRMQTLQRLYTTGCNIPIFVAGHKNIQAIQRPNQDFKWHNLFDEDDVLGWP
                     LRPLSPSYEALVEDLPVNAGGGLLGAIATSWNPFSHEQYWRDSEVIEHLARTIKALA"
BASE COUNT          646 a         1274 c         1279 g          709 t
ORIGIN      
        1 gacatcggcc tgatcgaagg cggcatctgc aacgccgaga acatccacgt gctgcaggag
       61 ttccgccgcc agtgcaaggt gctgatcgcc atcggcgcct gcgcggtgaa tggcgggctg
      121 ccggcgcaac gcaaccacct cagcctcggg cagtgcctgc aggaggtcta tctgacctgg
      181 ccgggtatcg tcagcgggca gatccccaac gatcccgaac tgccgctgcc gctcgacaag
      241 gtgcacccga ttcatgagat cgtgcgcatc gattacttca tccccggctg tccgccttcc
      301 ggcgacgcca tctggaagtt cctgaccgac ctgctcgacg ggcggacgcc gcggctggga
      361 catccgctgc tgcatttcga ctgagggaca ccgccatggc atctgaggct ttcgcactcg
      421 aaaccgccgc cgccgaagcc ggtacgctgc gccgggtggc catcgatccg gtgtcgcgcg
      481 tcgaagggca cggcaaggtc acgctgctgc tcgacgccaa cgacgaggtg cagcaggttc
      541 gcctgcacat cgtcgaattc cgtggtttcg agaagttcat ccaggggcgc ccatattggg
      601 aagtgccggt gatggtgcaa cggctgtgcg gcatctgccc ggtctcgcat cacctggcgg
      661 cggccaaggc gctcgaccac gtcgtcggcg ccgagaaact gccggcgagt gccgagaagc
      721 tgcgccgcct gatgcactac ggccagatcc tgcagtcgca cgccctgcat ttcttccatc
      781 tctcgtcgcc cgacctgctc tttggctttg ccagcgatgc tggccgccgc aacatcgtcg
      841 gcgttgccgc ggcgtatccg gaagtggcaa aacagggggt gctgttgcgc aagttcggcc
      901 aggaggtgat ccgcctgacc gccggcaagc gcgtgcatgg caccggcgcg attccgggcg
      961 gcgtcaatcg cgccctgagc cccgccgagc gcgacggcct gctggccgat atcgaccaga
     1021 ttgtcgactg gagccgcgcg gcggtgcaga tcgtgaagcg cctgcatgcc gccgatccgc
     1081 cgctctacga cagtttcggc accttccgct cgaacatcct ttcgctggtc ggcgccgacg
     1141 gcgcgctcga tttctacgat ggccgcctgc gcgctcgcga tgccgacggc aagatcctgg
     1201 tcgacggttt cgccgtcgag aattaccagc aactgatcca cgaggaagtc aagccgtgga
     1261 gctacatgaa gtttcccttc ctggccgcgc tcggcccgcg cgacggctgg tacaaggtcg
     1321 ggccgctggc gcgcgtgcag aactgcgatt cgatcgccac gccgctggcc gaaggcgagc
     1381 gccgcgagtt catcgaccat ggccagggtc gcctgatcca cgcgccgctg gcctatcact
     1441 gggcacgcat gatcgagatg ctgcacgcgg cggaaacgat caaggatctg ctgcacgacg
     1501 acgacctttc ggggagcgaa ctgagctgca gcggtacccg ccggcacgag ggcgtcggcg
     1561 tcatcgaagc gccgcgcggc acgctgatcc accactaccg cgtcgacgac gacgacctgg
     1621 tgacgatgtg caacctgatc gtctcgacca cgcacaacaa ccagccgatg aacgaggcca
     1681 ttcggcgtgt cgcccagcgc tatctcaacg gtcgccagct cagcgaaggc ctgctcaacc
     1741 acatcgaagt cgcgatccgc gccttcgatc cctgtctttc gtgcgccacg cacgcgctcg
     1801 gcaagatgcc gctgcaactc gaactgcagg atgccgcggg gcaggtgatc gcgcggatgc
     1861 gcagcgcctc ctgactttcc ggcggtgccg cccgtgtcgc tcgcgccggt ggtcattctg
     1921 gcctgcggca atcccagccg cggcgacgac gccctggggc cgatgctcct cgaccgcctg
     1981 caggactggc ttgccggcga gggcctgagc accggctttg aactgatcgg cgacttccag
     2041 tggcaggtcg agcacgccct cgacctcgtc gggcggcgcc tggctctatt cctcgatgcc
     2101 ggccagcgga cgccggcgcc gtgggttttt cggcaggtgc aggcgcttgc cacgccgacg
     2161 ccgagctcgc atgcgctgtc gccggaagcg gtgctcgccg tgctggcgcg catcggacaa
     2221 ccggcgccac cgaccttcgt gctcggcgtt gccggcgagc gcttcgaact cggggaaggc
     2281 ctgagcgccg ccgcgagccg ccacgccgag ggggcgttcg cactgctgca ggtcttgtgc
     2341 cgtactcccc gtgtcgatgc ctggcaggct ttggccaccg ggagaccggg cgggatcgtg
     2401 cgttggccgg cgagggatgc ggaatgagca ccccgttcgt ttctcgccgg gacgcggggt
     2461 tatgcgtccg gagcgagcgg cactcgcgtc ggagcgctta cccgggcttg gcgatgatcc
     2521 ccggcaggta gccggtcccc tggaagatgg agagattctg ccgacgcggc accattcccg
     2581 gagcggccag tccgaggtgt acccacgcgc cgtattcgtg gatgagctga tcgtaggcga
     2641 ttcctgacgc ggcgatcttt ttggctgttt gcaggacggt gccgaatttc ggggccgtaa
     2701 agtccgcagc cagacccagc aggtgcgcgc tctttgccga gccaccgacc gcggcgttga
     2761 gcgccgggct acggtaaccg ctactgatca acaggctcac gccgccgagc agcgtgcgaa
     2821 cttcctcgag aacctcggca aggcttttca ggttgcgcag tgccgccgct cccggcgtgt
     2881 tgtcgatccc acttctcgtg gcggtttggc tggcgaccag ttcatccagc gtaaagtgtt
     2941 cagtcaggct cctgggcatg tctcgctcct cctctgaata ccaaaaaatt gcgcacgtgg
     3001 tccgggatgg ccgggaccgt caggccagcg ccttgatggt tctcgccaga tgctcgatga
     3061 cttccgaatc ccgccagtac tgttcgtggc tgaaggggtt ccacgaggtg gcaatcgccc
     3121 cgagcaggcc gccaccggcg ttgaccggaa ggtcttccac cagggcttcg taagaaggac
     3181 tcagcggtcg cagcggccag ccgaggacat cgtcttcatc gaagaggttg tgccatttga
     3241 agtcctggtt cggccgctgg atcgcctgga tgttcttgtg cccggcaaca aagatgggaa
     3301 tgttgcagcc cgtcgtatac aggcgctgca aggtctgcat ccgccggaac tggtcttgcg
     3361 tggatccggc ggccaccccg tctcccggcg gcgcacgcca gatgcccgct gaagggcgcg
     3421 gctgctgcgc gtcccagatg tagctggaga tgacctggca tccgagcgac tgggcaacga
     3481 cgatcaccgg cacggcgctt cggcccgctt cctcgaagac gctgtccagg gtctggagga
     3541 tgatcttctg ggtctcgaaa tagggactgc cggcgacatc cttgcggtac tccaggctgg
     3601 ctgcgtccga gaatccatag agcaggaacc gccgcagttt catccagtcg atctggtcgc
     3661 gcatcctgcc gaaaatctcg gcctggttgc cttgaaggac attctggtag tagatgggcc
     3721 ggaaggcgat cctgttccag gtcgagttgc ccaaggcctt cttgagcagg tcatggagtt
     3781 cgacgtagta agtgggagag gtgtcgccca ttccatgcag ggtgagcagt gcaagtttct
     3841 ttggcatacg gattcccgaa aaaatcgatt ggcagagaac agcgtgctct tgccaggtgg
     3901 caagcggc
//
LOCUS       DAOSIT010000184         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_20752_length_3863_cov_11.982405, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000184 DAOSIT010000000
VERSION     DAOSIT010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20752_length_3863_cov_11.982405"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PLL14_05680"
     CDS             complement(<1..1691)
                     /locus_tag="PLL14_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOG03447.1"
                     /translation="MTFKPKLPAFLSRATDPAGETLLVRSTPMEPGEPESKGKVPLSL
                     AFLQRYPVTQQLQILGGCLVLVMLFIAVTVYRDSRQATYNTAYIATAGEMRMLSQRLA
                     KASSLALLGDPLAFKQLAESRESFASNLERLTSGGELAGTQVPPSPDGVQAQLVALTQ
                     LWEKTDKNAARLLEMEKNLISLGKEVAIINDKNPQLLDLAEQIAALKLQSSSGVREIA
                     AANLLVMLTQRIAKNASALLVGDAIDPEITFLLGKDTNTFRDTIHALSKGSDTLRIAG
                     TNDPDTKLKLGELEASFAEYRTAVGGILGNMQKLIIAKQAGSQIFRDSEELLTATDEL
                     AHSYQSSVLKRTAYGVVLLVLILLATGLVVLLAATYLAETRRQTEQAEQARHETELIN
                     RQNQDAILRLMNELGDLADGDLTVTATVSEDITGAIADSINYTIEELRVLVGRINDAA
                     GRVTLATEIAQQTSTELLAAAERQASEIKFAGQSVLAMASSMTGVSGDAKQSATVARQ
                     SLAAASKGAQAVQDSIKGMNKIREQIQETSKRIKRLGESSQEIGEIVELISDITE"
     gene            complement(1764..2273)
                     /locus_tag="PLL14_05685"
     CDS             complement(1764..2273)
                     /locus_tag="PLL14_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG03448.1"
                     /translation="MTKKISLHEFQSYLAARLSGTSNQSSAGLLGIQAGSDFWLLDLA
                     DSGEIVPLSPLTKVPLTKPWFAGIANIRGNLYSVVDLSAFLGKDVTPQNTSSRLLLIG
                     TRHGSNAALLVTRMIGLRNVEALNPEPQNGAAPAWALEAYTDNEGRRWNKLKVRELLA
                     DEAFMDIGA"
     gene            complement(2288..2650)
                     /locus_tag="PLL14_05690"
     CDS             complement(2288..2650)
                     /locus_tag="PLL14_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03449.1"
                     /translation="MTIKKILVVDDSPTERHVLKVLLTSNGYEVLTAESGEEGIAKAK
                     SELPDLILMDVVMPGLNGYQATRTLTRDPTTRNIPVIVCTTKGQETDKIWGLRQGAQD
                     YLVKPVNGQELLAKIAAL"
     gene            complement(2696..3091)
                     /locus_tag="PLL14_05695"
     CDS             complement(2696..3091)
                     /locus_tag="PLL14_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03450.1"
                     /translation="MAEAASPRGVKVMVIDDSNTIRRSAEIFLVQAGCQVLLAEDGFD
                     ALAKVADHQPDVIFCDIVMPRLDGYQTCALIKKNQRFRATPVIMLSSKDGLFDRARGR
                     MVGSDHYLTKPFTKDSLLQTVATFAPPTA"
     gene            complement(3182..3370)
                     /locus_tag="PLL14_05700"
     CDS             complement(3182..3370)
                     /locus_tag="PLL14_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG03451.1"
                     /translation="MNPASAETYRTWMCLTCGFLYEEAAGLPEEGIAAGTRWPDVPIN
                     WTCPECGARKEDFEMIEI"
     gene            3484..>3863
                     /locus_tag="PLL14_05705"
     CDS             3484..>3863
                     /locus_tag="PLL14_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HOG03452.1"
                     /translation="MLPRTLQQSPPIVLTFAASDPSGGAGIQADLMTLSSLGCHPLSV
                     TTALTVQDTVGVGCVLAIDSDCVEDQARTLLEDMPVAAFKIGLLGSIENIVVIAEILA
                     DYPGLPVVLDPVLASGRGDELADE"
BASE COUNT          666 a         1162 c         1169 g          866 t
ORIGIN      
        1 tgctcggtga tgtcggagat cagttcgacg atttcaccga tctcctgtga cgactcgcct
       61 agccgcttga tgcgcttcga ggtttcctgg atctgctcgc ggatcttgtt catccccttg
      121 attgagtcct ggaccgcctg cgcacccttg ctcgcggccg ccagcgactg ccgcgcaacg
      181 gtcgccgact gcttcgcgtc gccggacacg ccggtcatcg aactggccat ggccagaacc
      241 gactggccgg caaacttgat ctcggaagcc tggcgttcgg cagctgcgag caactcggta
      301 gacgtttgct gggcgatctc cgtcgccagc gtcacccgcc cggcggcatc gttgatgcgg
      361 ccgacaagga cccgcaattc ttcgatcgtg tagttgatcg agtcggcgat ggcgccagtg
      421 atgtcttcgg acacggtagc ggttaccgtc aggtcgccgt cggcgaggtc gcccagttca
      481 ttcatcagtc gcaggatggc gtcctgattc tggcggttga tcaactccgt ctcgtggcgg
      541 gcctgttctg cctgttcagt ctggcgtcgg gtttctgcca ggtaggtcgc ggccagcagg
      601 acgaccagac cggtggccag cagaatcagc accagcagga cgacgccata ggccgtcctt
      661 ttcaggacgc tgctctggta ggaatgcgcc agctcgtcgg tggcggtaag taactcctca
      721 ctgtcccgga agatttgcga gcccgcctgt ttggcgatga tcagcttctg catgttaccc
      781 agaatgccgc caacggcggt gcgatactcg gcaaaactgg cttcgagttc gccgagcttg
      841 agcttggtat cggggtcatt ggtgcctgcg atacgcagcg tgtcgctgcc tttggacagg
      901 gcgtgtatcg tgtcgcgaaa ggtattcgtg tccttgccca ggagaaaggt gatttccggg
      961 tcgatggcat cgccaaccag cagtgcgctg gcgttcttgg cgatccgttg cgtcagcatg
     1021 accagtagat tggcggctgc aatctcacgg actccggaac tgctttgcag cttgagtgcg
     1081 gcgatctgtt cggccaggtc gagcagttgc ggattcttgt cgttgatgat cgcgacttcc
     1141 ttgcccagcg agatcaggtt tttctccatt tcgagcagtc gcgccgcgtt cttgtcggtc
     1201 ttttcccata actgcgtcaa cgctaccaac tgtgcctgca cgccgtccgg agaaggcggc
     1261 acctgggtgc cggccaactc accaccgctg gtcaggcgct caaggttgct ggcgaaactt
     1321 tcacgcgatt ctgccagttg cttgaaggcc aggggatcgc ccagcaaggc cagactcgaa
     1381 gccttggcca gtcgctgcga aagcatccgc atttcgccgg cggtggcgat ataggcggtg
     1441 ttgtaggtcg cctgccggct gtcgcggtag accgttacgg caatgaacag catgaccagt
     1501 accaggcagc cgccaaggat ctgcagttgc tgggtcaccg ggtaacgctg caggaaagca
     1561 agcgacaacg gcactttccc tttactctcc ggctcgccgg gttccatcgg tgtgctacgc
     1621 accaacaggg tttcgccggc cgggtctgtc gcccgcgaca ggaatgccgg cagcttgggt
     1681 ttgaaggtca tcttgcttct ccggttacac agccttggcc gcgacgatcg tcgccgtcag
     1741 ggactccgtg atacgacagc ggctcaagcg ccaatgtcca tgaacgcctc gtccgccagc
     1801 aactcgcgta ccttgagttt gttccagcgg cggccttcgt tgtccgtata ggcttcgagt
     1861 gcccaggcag gtgcggcgcc attttgcggt tccggattca aggcctcgac gttgcgcagg
     1921 ccgatcatgc gcgtgaccag cagggccgcg ttgctgccat ggcgggtgcc aatcagcagc
     1981 agtcgcgaac tggtattctg gggggtgacg tccttgccca gaaaagcgga caggtcgacg
     2041 accgagtaca gattgccgcg aatgttggcg atccccgcga accagggttt ggtcagcggc
     2101 accttggtca gcggactcag cggtacgatt tcgcccgagt ccgcgagatc gagcagccag
     2161 aaatccgaac ccgcctgaat gccgagaagt ccggcagaac tctgattgct ggttccggaa
     2221 agccgggccg cgaggtagga ctggaattca tgcaggctga tcttcttggt catgccccgg
     2281 ccgccgtcta gagcgctgcg atcttggcca gcaactcttg accattgact ggcttgacca
     2341 ggtagtcctg ggcaccctgg cgcaagcccc agatcttgtc ggtctcctgg cccttggtcg
     2401 tacacacgat gaccgggatg ttccgtgtcg tcggatcgcg ggtcagggta cgggttgcct
     2461 ggtagccgtt caggccgggc atgacgacgt ccatcaggat caggtcaggc aactcgctct
     2521 tggccttggc gataccctct tcgccgctct cggcggtcag cacctcgtaa ccattgctgg
     2581 tcagcaggac tttcaagaca tgacgttcgg tcggtgaatc gtcaaccacg agaattttct
     2641 tgatggtcat ggctctctgc ttttcgcagc cccctctcgg caagttcggg ttttctcagg
     2701 ctgttggcgg cgcgaaggtg gcgaccgttt gcagcaggct gtccttggtg aatggcttcg
     2761 tcagatagtg gtcggagccg accatgcgcc cgcgtgcgcg gtcaaacagg ccatcctttg
     2821 acgacagcat gatcaccggc gtggcgcgga aacgttgatt cttcttgatc agggcgcagg
     2881 tctgatagcc gtcgaggcgc ggcatcacga tgtcgcaaaa gatcacgtcg ggctggtgat
     2941 cggccacttt ggcgagagca tcgaagccgt cctcggcaag caacacctga cagccggcct
     3001 gcaccaggaa aatctccgcg ctacgccgta tggtattgct gtcatcgatg accatgacct
     3061 tgacgccgcg cgggcttgca gcttcagcca attcactcct ccagggtgtc cccagtcatt
     3121 attctaatga agtcccggat ccgacatcat ctcctgccgg cgttactata ccgcattgtc
     3181 ttcagatctc gatcatttcg aaatcttcct tgcgtgcccc gcattccgga cacgtccagt
     3241 tgatcggtac atccggccag cgagttcccg cggcgatgcc ttcttcaggc agtcccgcag
     3301 cttcctcgta gaggaaacca caggtcaggc acatccatgt gcggtaggtt tcggcgcttg
     3361 ctgggttcat ggtcgctgtc ggttttcggt tagaatcgaa aaatcatcga gattctagcc
     3421 aaacccttgg gttgctgcca agactttgtg caccagtcca ctccgctacc cagacttttc
     3481 ctcatgcttc cacgaactct ccaacagtcg ccgcccatcg tgctgacctt tgccgccagc
     3541 gatccctccg gtggcgccgg tattcaggcg gacttgatga ccctgtcttc gcttggctgc
     3601 cacccgttgt cggtgacgac ggcgctgacc gtgcaggata ctgtcggcgt cggttgcgtc
     3661 ctggccatcg attccgattg tgtcgaggat caggcgcgaa cgttgctgga ggacatgccg
     3721 gttgccgctt tcaagatcgg cctgctgggc agcatcgaga atatcgtggt catcgccgaa
     3781 atccttgcag attaccctgg cttgccagtg gtgctcgacc ctgtgctggc ttccgggcgg
     3841 ggcgacgaac tcgcggacga gga
//
LOCUS       DAOSIT010000185         3808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21109_length_3808_cov_9.090861, whole genome shotgun sequence.
ACCESSION   DAOSIT010000185 DAOSIT010000000
VERSION     DAOSIT010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21109_length_3808_cov_9.090861"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLL14_05710"
     CDS             <1..257
                     /locus_tag="PLL14_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03453.1"
                     /translation="KAYLEYVREESKRCFGQGLNALEASKKIDFGPYGGWKAPARLYM
                     NVERAYREFRNEAAGAPWDHAKVFDAVLAVAKAKGIAVEF"
     gene            343..711
                     /locus_tag="PLL14_05715"
     CDS             343..711
                     /locus_tag="PLL14_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG03454.1"
                     /translation="MKFPRIYADEQGETHIGVCDRPEHDAPFGPPPTPMGLMTAIEGV
                     SSLVMFSVPGGTEVPAHNAPQPYICIVLSGEGEVTASDGSTVRLGPGGLLYCDDVSGK
                     GHVTRSITDLSLAFINRASS"
     gene            834..1274
                     /locus_tag="PLL14_05720"
     CDS             834..1274
                     /locus_tag="PLL14_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03455.1"
                     /translation="MKPRYAQFNPKRKLLSPAEAEVRVAEFQALASRARYGGNPEHKK
                     NPSDFGLVPPSDPRQGKSLCDVAKIFKRNEAEELLCAGLRKCLVSDRMVGDWPKNVWA
                     VTDDGIAMEAQLENPELGTYHGYPMPETDPLSLEVIRRWRCLNG"
     gene            1267..2586
                     /locus_tag="PLL14_05725"
     CDS             1267..2586
                     /locus_tag="PLL14_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03456.1"
                     /translation="MAELRINAKWMASASGEPEVMATAAWVEMYVDNISLTRNQDIWA
                     ETVRDNVFVSAYPLAMWFASSWWRLNHEPLPTQQPGHDWRMVHELGAANHGFVWPRAI
                     FIPDGEAIHVWAGASMTPEQSVQYLQALEVPRMITLTDFQQSVERFIHSTLARLDAKG
                     LSGSNLAHLWALVQEDLAGPEAHRRRKLEAEMGFDPEECPEQALSAALLWESQIGEAA
                     LSELAPAIAASGGTPDLALIGKLASAGGIVGTPDITPDSIDHLDHGAPWERAIHDARA
                     LRHRMGSVSGPVPDRSLHDLLGMSSDAAANWSAPVGRSPVAVAIPLNDHQLKFVPRRR
                     NSVGRRFELARFLGEYLRPSTHEARWLASTDLSTFRQKYQRAFAAEFLCPIDSLTSFL
                     GGEFSSYAFEEAAAEFDVSEQTITSLLLNNGYIHHPWTEGMPYRMAA"
     gene            2794..>3808
                     /gene="brxD"
                     /locus_tag="PLL14_05730"
     CDS             2794..>3808
                     /gene="brxD"
                     /locus_tag="PLL14_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HOG03457.1"
                     /translation="MISPQRRAEVIDALRRGTVPKAGLDALAVGYGRLQGTLDEELAA
                     VASGRGAFKAIRGEYGSGKTFFGRWLQERARARGLATSEVQISETETPLHRLETVYRR
                     LVERIATADSGEGAFRGIVDGWFYALERDVLEDASLDPTDEATLLARTVALMEARLAS
                     VTRVAPAFSATLRAYRRALQAGDNATADGLIAWLGGQPNVAAAIRRSAGVKGDIDHFG
                     ASNFLSGLLTMLRDSGYGGLLLVLDEVETLQRMRADTRERGLNALRQWIDEIDAGHFP
                     GLYLLITGTQAFYEGAQGVQRLAPLAQRLHVDFATDSRFDNARAVQVRLPAFGHDSLL
                     EVGR"
BASE COUNT          742 a         1117 c         1224 g          725 t
ORIGIN      
        1 tgaaggccta cctcgaatac gtgcgcgaag aatcgaagcg ctgcttcggg cagggtctga
       61 acgccctgga ggcgtcgaag aagatcgatt tcggaccgta cggcggatgg aaagccccgg
      121 ctcgtctgta catgaacgtc gagcgggcct accgcgagtt tcgcaacgag gccgcgggcg
      181 cgccctggga tcacgcgaaa gtcttcgacg cggtccttgc cgtggcgaag gccaagggga
      241 tcgcggtcga gttttgattg gggtacacgt ttccattcaa tccgggacgt tcccggtgac
      301 ggcgcgcacg aaaaccaaac atcgatagaa ggagaaacag aaatgaaatt tccgcgtatc
      361 tacgccgacg aacagggtga aacgcacatc ggcgtctgtg accgcccgga gcatgatgca
      421 ccgttcgggc cgcctccaac tcccatgggg ctgatgacag ctatcgaggg ggtcagtagc
      481 cttgtcatgt tctcggtccc gggcgggacc gaggtgccgg cccacaatgc gccacagccc
      541 tatatctgca tcgttctctc cggcgagggc gaggtgaccg ccagcgatgg ctcgaccgta
      601 cgcctgggtc ctggtgggct gctctactgc gacgacgtca gcggcaaggg ccacgtgacc
      661 cggtcgatca ccgacctcag ccttgccttc atcaatcgtg ccagttcgtg agccggcagc
      721 gagcacaccg acaatcaaac ctgcagcgca gagacgaggg gatccatgat gatggcctgt
      781 accattgaat taactggtaa actcgagagc ggtaatggtc tgccctaaac acgatgaagc
      841 ctcgctacgc ccagttcaat cccaaacgca aactactctc gcccgctgag gcagaagttc
      901 gcgtggccga attccaggca ttggccagtc gcgctcgcta tggcggcaac ccagagcata
      961 agaagaaccc cagcgatttt gggctcgttc caccttccga tccaagacaa gggaaatcgc
     1021 tgtgcgatgt cgccaagatt tttaagcgta acgaagctga agagttactt tgcgcgggac
     1081 tgcgcaagtg ccttgtgagc gaccggatgg ttggcgattg gccgaagaac gtatgggcag
     1141 tcactgacga cggtattgcc atggaagcgc agcttgaaaa ccccgagttg gggacctacc
     1201 atgggtaccc catgcctgag actgatccgc tgtcgctgga agtcattcgt cgttggaggt
     1261 gcctcaatgg ctgagctgcg catcaacgcc aagtggatgg catcggcatc cggggaacca
     1321 gaagtcatgg ccacagcagc ctgggtcgag atgtatgtcg acaacatcag cctgactcgg
     1381 aaccaggaca tttgggcgga gaccgtgcgt gacaacgtct tcgtttctgc ctacccgctg
     1441 gcaatgtggt ttgcgagttc gtggtggcgc ctcaaccatg aacccctgcc aacgcagcag
     1501 cctggccatg actggcgtat ggtccatgag cttggtgcag ccaatcacgg cttcgtgtgg
     1561 ccgcgcgcca tctttatccc ggatggtgag gcgattcacg tgtgggccgg agcatcgatg
     1621 acgccggagc agtcagtgca gtacctgcaa gcgctggaag tgccgagaat gatcacgttg
     1681 actgactttc agcagagcgt cgagcgcttc atacacagca cactggcccg cttggatgcc
     1741 aaggggctct ctgggtcgaa tttggcgcac ctgtgggccc tcgtgcagga agatttagcg
     1801 ggccccgagg cgcaccgccg gaggaagttg gaggccgaaa tgggattcga tcccgaggag
     1861 tgtccggagc aagcgctgag cgcggcattg ctgtgggaga gccagatcgg agaggcggca
     1921 ctgtcagagc tggcgccggc gattgcagcg tcgggaggta ccccggattt ggcgctcatt
     1981 gggaagctgg ctagtgccgg cggaattgtt ggcacccccg acatcacgcc tgacagcatc
     2041 gatcacttgg accacggggc accttgggag cgcgcgattc acgacgctcg tgcgcttcgt
     2101 cacaggatgg gcagtgtcag cggtccagtt cccgaccgca gtctgcatga cctgcttggc
     2161 atgagcagtg acgccgcagc caattggtcg gcgccggtag gacggtcgcc tgtagcggtg
     2221 gcgataccac tgaacgatca ccaattgaag tttgttccgc gcaggcgcaa ctctgtcggg
     2281 cggcgcttcg agctggcgcg tttcttgggc gagtacctcc gtccctcgac ccacgaagcg
     2341 cgttggcttg catcaaccga cctatcgact ttccgtcaaa agtaccagcg cgctttcgcc
     2401 gcagagtttc tttgccccat cgattcatta acgtcctttt tgggcggtga attctcttca
     2461 tatgccttcg aagaagccgc cgccgagttc gatgtcagcg agcagacgat aacaagcctg
     2521 ttgctcaaca atggctatat tcatcatcct tggactgagg gcatgccgta tcgaatggca
     2581 gcctgatctg cttttgatga tcataaagcc gtcgtagcct ttgcagacgt catcaggctg
     2641 ttgtgggtcg aatacggtcg ttcgcaatcc aaggagacat cagttcatca gtggtggtca
     2701 gaacccctgg tcgagtttgt atcgttatcg ccagcggctt tccatcgatt cggcgaaccg
     2761 gttcggaatg acgtacgcaa tgagggttta caaatgatca gtccgcagcg cagagccgaa
     2821 gtcatcgacg ccttgagacg cggcacggtg ccgaaggccg gcctggatgc gctggccgtc
     2881 ggttatggcc ggctgcaggg aacgctcgac gaagagttgg ctgccgtggc gtccggccgc
     2941 ggcgctttca aggcgatccg cggcgaatac ggatcgggca agaccttctt cggccgctgg
     3001 ctgcaggagc gggcgcgggc gcgcggcctg gcgaccagcg aagtgcagat ctcggaaacc
     3061 gagacgccgc tgcatcgcct ggaaacggtg taccgccgcc tggtcgagcg catcgccacg
     3121 gcggacagtg gcgaaggcgc atttcgcggc atcgtcgatg gctggttcta cgcgctggag
     3181 cgtgacgtgc tggaagacgc cagcctggac ccgaccgacg aggcaaccct gcttgccagg
     3241 accgtggcgc tgatggaggc gaggctggcc agcgtgaccc gggtggcgcc ggccttttcg
     3301 gcgacgctgc gcgcctaccg tcgcgcattg caggccggcg acaacgccac ggccgacggg
     3361 ctgatcgcct ggctcggtgg ccagcccaat gtcgcggcag cgatccgccg atcggccggg
     3421 gtgaagggag acatcgatca cttcggcgcg agcaacttcc tgtccgggtt gctgacgatg
     3481 ctgcgcgact cggggtatgg cggtctgcta ctggtgctcg acgaagtcga gaccctgcag
     3541 cgcatgcgtg ccgacacccg cgagcgcggg ctgaatgcgc tgcgccagtg gatcgacgaa
     3601 atcgacgccg gccattttcc ggggctgtac ctcctgatca ccggcaccca ggctttctat
     3661 gagggggccc aaggcgtgca gcgtctcgcg ccgctggcgc aacgactgca cgtcgatttc
     3721 gcgaccgact cgcgcttcga caacgcacgc gcagtgcagg tgcgcctgcc ggccttcggt
     3781 cacgattcgc tgctcgaagt gggacgca
//
LOCUS       DAOSIT010000186         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21143_length_3803_cov_9.472519, whole genome shotgun sequence.
ACCESSION   DAOSIT010000186 DAOSIT010000000
VERSION     DAOSIT010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21143_length_3803_cov_9.472519"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..603
                     /locus_tag="PLL14_05735"
     CDS             85..603
                     /locus_tag="PLL14_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG03458.1"
                     /translation="MQIAEDRLGEARQLAEACVKPPVMVAVPAPPPPPAPPPPPPRVV
                     REKIVISAAALFRFSRGGRADLLDDGRAKLDELASRLGSAYSRVDEIALTGYTDRLGG
                     DTRNARLSLQRAESIKAELLARGVTAPITTAGRGAAQPVVQCEGKQATPALTACLQPN
                     RRVEVEVVGVRR"
     gene            655..1125
                     /locus_tag="PLL14_05740"
     CDS             655..1125
                     /locus_tag="PLL14_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG03459.1"
                     /translation="MNLTTDFFSDPTRLAPRRTVRTNLPGGGFTLQHPEPLAPYARCV
                     GEWLEHWARETPESIAFAEPNTAAASAASSAWSTLTWAGLRARVGAVAQSLLDLKLAP
                     GRPIVVLSDNSLEHLVLMLAGMPSPGSSASYGQRSARSRWAASGPPTCSRSSRT"
     gene            1101..1946
                     /locus_tag="PLL14_05745"
     CDS             1101..1946
                     /locus_tag="PLL14_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOG03460.1"
                     /translation="MLAIIEDLRGTPKTAEGVRQHIQQIYNYAIQKLLVEVNPALPLR
                     GVIEVPAAEHHRHLTEPELGAFWRSVAKQGAHFVTIAATRMLMYTMCRKSEVLRARWR
                     EFDLERAQWDIPAERMKMKAPHRVYLSRQALELLGLLKAFGSEPQAYVFPSVLSAAVP
                     LGDATLNHFFKRLDFGVPDFSPHGTRGTAATLLREHGFSREVVELLLAHAERNKVTAA
                     YHHHEMADERRRALQYLADQVDRLAMAGSAPPVSSHDLPGPTAVAPIGRLSLADRNDS
                     ARRRG"
     gene            complement(2328..>3803)
                     /locus_tag="PLL14_05750"
     CDS             complement(2328..>3803)
                     /locus_tag="PLL14_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HOG03461.1"
                     /translation="VGYLAISIPFIAWAALNRMETFGTALVGGLSGLQSMVAGATAGA
                     AAGNLSMGNVSMDQIRLAPNRTSAFMSNWQNDISGNTFSSNMLTGRTAVSLLRNQGYA
                     SRVVSMRVSEQDVQDASRQVDAARSEAVAASTERSAVLSEAFTKGLAKLKSSRSSTGS
                     TSSSFEQLGQTLNRLDQITRSVADSTGMSQSQVANIAFGAAAHLGLSTAVAGGKLNAT
                     ADKSYLSGLTVQEQKVLGSMTSEQLSEFKQFGDRVSRDSSFSTAVAADAREATEIASR
                     INSSATRTTRAEAALAQRTSYSERLATALERGEVISIDLAQDPHNLAMFTRYAEQYGG
                     TSAAAQALFEAELARRALGPNRTFTDGTGVPVTFGSLPPEHLRQSAIVDSTVDVPGMR
                     KDSDAAVWRTRGNPSPPTAPAESPELRRDQVRTEATALRQRAGAQADQLGRNIEADRA
                     EDGTLVPRRSLLKQSAKQVGKDAATTAESAADAVKDILKRK"
BASE COUNT          598 a         1258 c         1248 g          699 t
ORIGIN      
        1 cacatactat ggagagtaca aggagtggta gtgtcccgtc aagtggtgta attccagcag
       61 ggctggcggc acgccaaacc ctatgtgcag atcgccgagg atcgcctggg cgaggcgcgt
      121 cagctggccg aggcttgtgt caaaccgccc gtgatggtgg cggtgcccgc gccaccccct
      181 ccgcccgcgc cgcccccacc cccgccgcgg gtggtgcgcg agaagatcgt catcagcgcg
      241 gccgcgctgt tccgcttctc gcgtggcggc cgcgccgatc tgctcgacga cggccgcgcc
      301 aagctcgacg agctggccag ccgtctgggc tcggcctaca gccgggtcga cgagatcgcg
      361 ctcaccggct acaccgatcg cctgggcggc gatacccgca acgcccgcct ctcgctccag
      421 cgtgccgagt cgatcaaggc ggagttgctc gcccgtggcg tcaccgcgcc gatcacgacc
      481 gccggtcgcg gtgccgccca gcccgtggtc cagtgcgagg gcaagcaggc cacgcccgcg
      541 ctcacggcct gcctgcaacc gaaccggcgc gtggaggtgg aagtggtggg cgtgcgccgc
      601 tagacgcgcg gcacagtcgt gccaccatcg gcgctcgcag ccgagggtgc acgagtgaac
      661 ctcaccactg acttcttctc cgaccccacc cgcctggccc cgcgccgcac cgtgcgcacc
      721 aacttgccgg gtggcggctt cacgcttcag caccccgagc cgctggcgcc gtatgcgcgc
      781 tgcgtgggcg agtggctgga gcattgggcg cgcgagacgc cagagtcgat cgccttcgcg
      841 gagcccaaca ccgcggccgc gtcagccgca tcgtctgcct ggagcaccct cacctgggct
      901 ggcctgcgcg cgcgcgtggg cgcggtggcc cagtcgctgc tcgacctcaa actcgcaccc
      961 ggccggccga tcgtggtgct gtcggacaac tcgttggagc acctggtgct gatgctggcc
     1021 ggcatgccga gtcccggctc gagcgcttcg tatggccagc gatcggcgag aagccgctgg
     1081 gcggcgtccg gcccgccgac gtgctcgcga tcatcgagga cttgagagga acgccgaaga
     1141 cggcggaggg cgtcaggcaa cacatccagc agatctacaa ctacgcgatc cagaagttgc
     1201 tcgtcgaggt caacccggcc ttgccactgc gaggcgtcat cgaggtgcct gctgctgagc
     1261 accaccggca tctgaccgag ccagaactgg gagccttctg gcggtcggtg gctaaacaag
     1321 gcgcgcactt cgtgaccatc gctgccaccc ggatgctcat gtacacgatg tgccgcaagt
     1381 cagaggtgct gcgagcccgg tggcgcgagt tcgacctcga gcgggcccag tgggacatcc
     1441 cggccgagcg tatgaagatg aaggcgccgc accgtgtcta cctttcccga caggcactcg
     1501 aactcctcgg gctgctgaag gcattcggct cggagccgca ggcctacgtg ttcccgtcgg
     1561 ttcttagtgc cgcggtgccg ctcggcgacg ctacgctgaa ccacttcttc aagcgactcg
     1621 acttcggcgt tccggacttc tcgccccatg ggaccagagg cacggcagcg accttgcttc
     1681 gtgagcatgg attcagccgc gaggtagtcg agctgctact ggcgcatgcc gagcgaaaca
     1741 aggtgacggc cgcgtatcac catcacgaga tggctgacga gcggcggcgc gcgctccagt
     1801 acctcgccga tcaagttgat cgactcgcca tggctggctc agcacctccg gtgtcttcgc
     1861 acgaccttcc tggtccgact gcggttgccc ccatcggtcg gctcagcctt gcagatcgga
     1921 acgactcggc taggcggcgc ggctagatgt gctggcactc gcagcaaggt gggagtacgc
     1981 tagcgcggtc gggggtgcgt gacatgcggt gggctcgttt catcggcgtg gtttgtctgg
     2041 gagctctgct cggcgtggtt gcagcgctgg aggacgacac gtctacgaag gttggcatgg
     2101 cgctcgtggg cgcattgttc gcgacgccga ttgcctccat agtggctcgc agtcgagggt
     2161 cgaaccctca cgtcggtgaa ccagggcatt ccatggacgg cgcaggctcg acgtcaccta
     2221 gtgccctcgc gcgaaactac tggcgtgacc gaggccatcc cccgtacatg aagccctctg
     2281 atggcgatcc ggacaagcac atgttcgatc ccgaccggct ctcctgacta cttccgcttc
     2341 aggatgtctt tgacggcatc ggctgcgctc tcggccgtgg tagcagcgtc tttgccaact
     2401 tgcttggcgg actgcttcag caaggaccta cgcggcacga gggtgccgtc ttcggctcgg
     2461 tcggcttcga tgtttcgacc gagctgatct gcctgcgctc cagcgcgttg ccgaagcgcc
     2521 gtcgcctcag ttcgcacttg atctcggcgc agctccggcg actcggcggg tgcagttggt
     2581 ggcgatggat tcccgcgagt tctccagacg gcggcatcag agtctttgcg catgccgggt
     2641 acgtcgacgg tcgagtccac gattgccgac tggcgaagat gctctggagg gagtgacccg
     2701 aacgtcactg gcacgccagt cccatccgtg aatgttcgat tcggcccgag ggctcgacgt
     2761 gcgagttcgg cttcaaagag agcttgcgcc gccgcactgg tgcctccgta ctgctcagcg
     2821 taacgggtga acatggcgag gttatgtgga tcctgggcca ggtcaatgga gatgacttcg
     2881 cccctctcaa gcgcagtggc caggcgttct gagtagctgg ttctttgcgc cagcgcagct
     2941 tcagcacggg tggtccgggt ggcagatgag ttgatgcgtg acgcgatctc cgtggcttca
     3001 cgtgcgtcgg ccgcaaccgc agtcgagaag ctcgaatctc tggacacccg atcgccgaac
     3061 tgcttgaact cgctaagttg ctcgctggtc atgctgccga gcaccttctg ctcctgcact
     3121 gtcaggcccg acaagtagct cttgtctgct gtggcattga gctttccgcc agcgactgcg
     3181 gtgctcagtc ccaggtgtgc ggcggcgccg aaggcaatgt ttgcgacttg cgactgagac
     3241 atgccagtgc tgtcggcgac gctcctcgtg atctggtcca accggttcag cgtctgcccc
     3301 aactgctcaa agctgctgga tgtagacccc gtgctgcttc gcgatgactt cagctttgcc
     3361 aagcccttgg tgaatgcctc ggacagcacc gccgaacgtt cggtgctggc agcgaccgct
     3421 tcgcttcggg ctgcgtcgac ctggcggctt gcgtcctgca cgtcctgttc gctcacgcgc
     3481 atcgagacga cccgcgacgc gtagccctgg ttgcgcagca ggctgacggc ggtccggccg
     3541 gtcagcatgt ttgacgagaa ggtgttgccg ctgatgtcgt tctgccagtt gctcatgaac
     3601 gcggacgtcc ggttcggcgc gagccggatc tggtccatcg agacattgcc catgctcaga
     3661 ttgcccgcgg cggcacctgc cgtggcgccg gccaccatcg actgcaggcc cgagagaccg
     3721 cccaccagcg cggtgccaaa ggtctccatc cgattgagcg cggcccaggc gatgaacggg
     3781 atgctgatcg ccaggtagcc gac
//
LOCUS       DAOSIT010000187         3802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21148_length_3802_cov_10.515346, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000187 DAOSIT010000000
VERSION     DAOSIT010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21148_length_3802_cov_10.515346"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /gene="dcd"
                     /locus_tag="PLL14_05755"
     CDS             complement(<1..364)
                     /gene="dcd"
                     /locus_tag="PLL14_05755"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOG03462.1"
                     /translation="MSIKSDRWIRRMAEQHGLIDPFEPQQVREIDGRRIVSYGTSSYG
                     YDIRCSREFKLFTNLNSTIVDPKNFDPNSFVEVTSDFCIIPPNSFALARTIEYFRIPR
                     SVLTICLGKSTYARCGIIV"
     gene            complement(496..1587)
                     /gene="apbC"
                     /locus_tag="PLL14_05760"
     CDS             complement(496..1587)
                     /gene="apbC"
                     /locus_tag="PLL14_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HOG03463.1"
                     /translation="MAIDTEIVQAALKDLIDPNTHRDYVSTRSARNIRIQGADVSLDI
                     ELGYPAQTQLDDIRGAVVGKLAAIPGIGKISVNVAVNIVAHTVQRGLKPLPGVKNIIA
                     IASGKGGVGKSTTAVNLALALAQEGARVGLLDADIYGPSQPQMLGLVGQKPESQDGVS
                     MDPLQAYGLQAMSIGFMIDIDSPMVWRGPMVTQALEQLLKQTNWHNIDYLLVDMPPGT
                     GDTQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGIPILGLVENMSIHICSQ
                     CGHAEHIFGHGGGEQMCKDYDTELLGSLPLELSIRELTDSGKPTVVGAPDSRAAEIYR
                     AIARRLAVKVAERTRDMSTRFPNIVVQNT"
     gene            complement(1724..2521)
                     /locus_tag="PLL14_05765"
     CDS             complement(1724..2521)
                     /locus_tag="PLL14_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG03464.1"
                     /translation="MLDASVALVREVAQREIIPRFLRVSHGQRKDDGSLCSEADLAAQ
                     HFLLDRLTEIHHCPIIGEEMTAAVQQARWHDSSDDDRGLWCIDPIDGTTNFINGLPCF
                     AVSIAWMRARRTRLAVTYNPITDEMFYAREGHGAYLNGRRLPLRQVTADIGRAVGGVD
                     FKRIPKLLADRIAVSPPFYSQRNFGSSTLDWCNLAAGRLDLYLHGGQMLWDYAAGSLI
                     LREAGGQMCTLEHDDYDADEVWRRPVIAALHPAVFEAWRNWVRQPHS"
     gene            complement(2582..3133)
                     /locus_tag="PLL14_05770"
     CDS             complement(2582..3133)
                     /locus_tag="PLL14_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG03465.1"
                     /translation="MASNTPVCDFGWQAVDFELEDTHASRHTLASLRGPNGLLLMFIC
                     NHCPYVKAIIDRICRDARDLQALGIGVAAIMSNDPGEYPEDSLENMQRVAEQLAFSFP
                     YLHDTTQAVARAYGAVCTPDFFGFNQQLQLQYRGRLDASGRLPAGPHVRRELFEAMRL
                     IAETGQGPREQIASIGCSIKWRT"
     gene            3190..>3802
                     /locus_tag="PLL14_05775"
     CDS             3190..>3802
                     /locus_tag="PLL14_05775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOG03466.1"
                     /translation="MDCPRFYCPLPLAVGERVELPARVAQHAVRVLRLRCGDALTLFN
                     GVGGEYRARIIDGVLARVSVEVHEWLATERESPLSVTLVQALQAGEKMDLTVQKAVEL
                     GVGRIVPVLSRRSVLRLEGDRALRRLEHWRGIVAAACEQCGRNRLPEVTAPESLEKWL
                     MRKPVNASVRLTLAPTAGCSLTTLPPAAGGAIELLVGPEGGLAP"
BASE COUNT          699 a         1101 c         1231 g          771 t
ORIGIN      
        1 tgacgatgat gccgcaacgt gcataggtgc tcttgccaag acagatggtc agcacactgc
       61 gcgggatgcg gaaatactcg atggtgcgtg ccagggcaaa cgagttcggc ggaatgatgc
      121 agaaatcgct ggtgacctcg acaaaggaat tgggatcgaa gtttttcgga tccacgatgg
      181 tcgagttcag attggtaaac agcttgaatt cccttgaaca gcgaatgtcg tagccgtagc
      241 tcgaggtccc gtaagagacg atgcggcggc cgtcgatctc tcgcacctgt tgaggttcga
      301 agggatcgat caagccgtgc tgctccgcca tgcggcggat ccacctatcc gatttgattg
      361 acatcgcgct tcacccggac accccgatat gggaaaggtc cccattctac ggtgtctggg
      421 aggcggaaga aactgctcgc taagctggcg ctcgcgaagc tggcgtttgg gccgtcgtcg
      481 cgcgcgccag cccgctcagg tgttctggac gacgatgttc ggaaaccggg tgctcatgtc
      541 tctggtccgt tcggcgacct tgaccgccag tcggcgagcg atcgcgcggt agatttcggc
      601 cgcgcgagag tcgggggcgc ctactaccgt gggtttgccc gagtcggtca actcgcggat
      661 cgaaagttcc agtggcaggg aaccgagcaa ctccgtatcg taatccttgc acatctgctc
      721 gccgccgccg tgaccgaaaa tgtgttccgc atgtccacat tgcgagcaga tgtggatact
      781 catgttctcc accaggccga gaatcgggat gcctaccttc tcgaacatct tcaggccctt
      841 gcgcgcgtcg atcagggcga tgtcctgtgg ggtggtgacg atcaccgcgc cggtcacagg
      901 cactttctgc gccagggtca gttgcgtgtc gccggtcccg ggtggcatgt cgaccagcag
      961 gtaatcgatg ttgtgccagt tggtctgctt gagcaactgc tcgagcgcct gggtgaccat
     1021 cggtccccgc cagaccattg gtgaatcgat gtcgatcatg aagccgatcg acatcgcctg
     1081 tagaccatac gcctgcaagg ggtccatcga gacgccatcc tgcgactcgg gtttctgtcc
     1141 caccaggcca agcatctgcg gttgggaggg gccatagata tcggcgtcga gcaacccgac
     1201 gcgggcgcct tcctgcgcca gtgccagcgc cagattcacc gccgttgtac tcttgccgac
     1261 accgcctttg ccggaagcga tggcgatgat gttcttgacg cccggcagcg gtttcaagcc
     1321 gcgctgcacg gtgtgggcga cgatgttgac cgcgacattg acgctgatct tgccgatgcc
     1381 cgggatcgcc gcgagttttc cgacgactgc gccgcgaatg tcatcgagct gtgtttgcgc
     1441 gggataaccc aactcgatat cgagtgagac gtctgcgcct tgaatccgga tgttcctggc
     1501 cgaacgtgtc gagacataat ctctatgggt attgggatcg atcagatcct tcagtgccgc
     1561 ttgtacgatt tcagtgtcga tagccattgg tcaaagctcc tgtggtagat actggactaa
     1621 tttactccag tttacaccaa aacgggctgt cgactgatcg cggcaagcct ccccgccacg
     1681 gatcatgcat ggttcctgcg ctccgccagg gcggggccga tcttcaggaa tgaggttgac
     1741 gcacccagtt tcgccaggct tcgaagactg ccgggtgcag tgcggcgatc accggccgac
     1801 gccatacctc gtcggcatcg tagtcgtcgt gctcgagggt gcacatctga ccaccggcct
     1861 cgcgcaggat cagactgccg gcggcatagt cccagagcat ctggccaccg tgcaggtaga
     1921 ggtcgagccg gccggcggcg agattgcacc agtcgagcgt cgaactgcca aaattacgtt
     1981 gcgagtagaa aggggggctt acggcgatgc gatcggccag cagcttcggg atgcgcttga
     2041 agtctactcc gccgacggcg cgtccgatat ccgcggtgac ctggcggagc ggcagccggc
     2101 ggccgttgag gtaggcgcca tggccttcgc gagcatagaa catctcgtcg gtgatcgggt
     2161 tgtaggttac cgccagacgt gtgcgccgcg cgcgcatcca ggcgatggaa accgcaaagc
     2221 acggcagtcc gttgatgaaa ttggtggtgc cgtcaatcgg gtcgatgcac cagagtccgc
     2281 ggtcgtcgtc gctgctgtcg tgccatctgg cttgctgcac cgcagccgtc atctcttcgc
     2341 caatgatcgg gcagtggtga atctccgtca gccggtcaag gagaaaatgc tgcgcggcca
     2401 ggtcggcctc cgagcacagc gagccgtcgt ccttgcgctg accatgactg acgcgcaaga
     2461 agcgcggaat gatttctcgc tgtgcgactt cccggaccag cgcaacactg gcatcgagca
     2521 tctgtttcag gtcactgtcg gcgattgccg cggtggtgtt cttagcattg cgcatgccga
     2581 gtcaggtgcg ccacttgatc gagcagccga tgctggcaat ctgctcacgc ggaccctgtc
     2641 cggtttcggc aatcaggcgc atcgcctcga agagctcgcg tcggacatgg ggccctgccg
     2701 gcaggcgtcc gctggcatcg agacgtcccc ggtactgcag ttggagttgc tggttgaagc
     2761 cgaagaaatc cggtgtgcat acggcgccgt aggcgcgcgc gacagcctgt gtggtgtcat
     2821 gcaggtaggg aaaggaaaag gccagttgtt cggccacacg ctgcatgttt tccagggaat
     2881 cctccgggta ttcgccgggg tcgttgctca tgatggctgc aacgccaatg cccagtgcct
     2941 gcaagtcgcg cgcgtcacgg cagatacggt cgatgatggc cttgacatag gggcagtggt
     3001 tgcaaatgaa catcagcagc aagccgttcg ggccgcgcaa agatgcaagg gtgtgtcgcg
     3061 aagcatgcgt atcctcgagt tcgaagtcga cagcctgcca gccaaagtca caaaccgggg
     3121 tattgcttgc catcaccaac atccttccaa acaacgacca ctgagccctt aagctcttat
     3181 aatagcacgg tggattgccc ccgcttttat tgcccactgc cgctggcggt cggagagcgc
     3241 gtcgaattgc cggcgcgggt cgcgcagcat gcggtcaggg tgctgcgcct acgttgtggc
     3301 gatgcgttga cgctcttcaa cggggttggc ggggaatatc gtgcccggat aatcgacggc
     3361 gtgcttgcgc gggtcagcgt cgaagtccac gaatggcttg ccacggagcg tgaatcgccg
     3421 ctttcggtta ctctggtgca ggcgctgcaa gccggtgaga agatggacct gacggtgcag
     3481 aaggccgtcg aactgggcgt tgggcgcatt gtcccggtgc tctcgaggcg cagtgtgctg
     3541 cgtcttgaag gcgatcgcgc cctgcgccgc ctggagcatt ggcgtggcat cgtggccgca
     3601 gcctgcgagc agtgtgggcg caatcggctg ccggaagtga cggctccaga gagcctcgaa
     3661 aaatggctga tgcggaagcc cgtgaacgcg agtgtgcgtc tgaccctggc gcccactgcg
     3721 ggatgttctc tgacgacgct gccgccggcg gccggcggcg caattgaact gttggtcggc
     3781 cccgaggggg ggttggcacc cg
//
LOCUS       DAOSIT010000188         3802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21150_length_3802_cov_6.439819, whole genome shotgun sequence.
ACCESSION   DAOSIT010000188 DAOSIT010000000
VERSION     DAOSIT010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21150_length_3802_cov_6.439819"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="ald"
                     /locus_tag="PLL14_05780"
     CDS             <1..914
                     /gene="ald"
                     /locus_tag="PLL14_05780"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOG03467.1"
                     /translation="MIIKVKEPQAGERAMLREGQILYTYLHLAPDPAQCADLVSSGAV
                     CIAYETVTQPGGGLPLLAPMSEVAGRLSVQAGAYCLEKAHGGMGVLLGGVAGVPSARI
                     VILGGGVVGSNAAQIAVGSGAQVVVIDRNLDVLRRMDRLLGARVITVFSNRDNVEHHV
                     LRADLVIGGVLIPGAAAPKLVTRQMVDAMKPGSVIVDVAIDQGGCCETAKATTHAEPT
                     YMVGKVVHYCVANMPGGVPRTSTYALNNATLPFALQIADKGWRQALQDNVHLRNGLNV
                     AFGKVTCREVAEDLGYLYHDALSVIAG"
     gene            1162..>3802
                     /locus_tag="PLL14_05785"
     CDS             1162..>3802
                     /locus_tag="PLL14_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOG03468.1"
                     /translation="MKTTGEGERTLDPLAAEDVAGRLSDALARQLSRILEDLGGTGEE
                     RAHRQLELINSILTDVRHRLGGHGNQIDTVHAPLQLLHSIHRCTPPRVLPDTGLAMPW
                     LFTAGKGSPSLLSELRRELAACDEVDILVSFITHSGVRRLLDILQSVTAIGADGQPRS
                     RLRVLTTTYTGATEIQAVDELARLPGCQVKVSLDGRRTRLHAKAWLFRRQTGFGSAYV
                     GSANLSGAALAGGLEWTVKFTERGQLELFERAKAHFETLWDDNEFQSYDPADIRHRRA
                     LSDALTRESGCDIVARPTFFDLEPKAYQQEMLDQLLIERERGRRRNLVVAATGTGKTV
                     VAAFDYRRECEQKGGRPRLLFVAHRLEILKQALRTFREVLRDHSFGELLVGGTEIDHF
                     DHLFASIDSLASRNLVQRCGADYWHVVVVDECHRLAAERFHNFVSVVRPHILLGLTAT
                     PERSDGQPILGYFDNRPDGAPAVELRLWHALDLQFLSPFEYYACDDDTDFSQVPWEQP
                     GELAAIDGLVTGNEVRARLVLNEWRRLAGDPARCRALVFCVSVAHALYMTQKLNQSGI
                     RALCVVGNTPDHERRRAPELLASGQVCALVTCDLYNEGVDLPSVDTLLLLRPTQSPVL
                     FQQQIGRGLRLAKGKESCLVLDFVGRHRQDFRFDRLLATITGLSRGQLIDAVDQGFGS
                     LPPGCHIHLQRQTRDQVLSSLKRLVQQNWRRLRAELQTYMALKGRAKVRLATFLREQA
                     VDLEDIYRGAGRSGWTNLKRDAGLLTTPTGPEDDYFGGRFADLLHFDDPARINLLLKV
                     GEPDASYFPEVKSEQRLLQMLAYQVDGQHHRTGRGADFLQRLSVQTDTRNELAEIAEV
                     LQGRSTLSHQAVPGLESTPLCLHA"
BASE COUNT          687 a         1110 c         1212 g          793 t
ORIGIN      
        1 aaatgatcat caaggtcaag gagccacagg ctggcgagcg agcgatgctg cgcgaaggcc
       61 agattctgta tacctatctc cacctcgctc ccgatccggc gcagtgtgct gacctggtca
      121 gcagtggcgc ggtgtgcatc gcctatgaaa cggtgacgca gcctggtggc ggcctgccgc
      181 tgctggcacc gatgtcggag gtcgctggcc gattgtcggt gcaggccggc gcctactgcc
      241 tcgaaaaggc gcatggcggc atgggcgtgc tgctgggcgg cgtcgccggc gtgccgtcgg
      301 cgcggatcgt catcctcggt ggcggcgtcg tcggctcgaa cgcggcgcag atcgccgtcg
      361 gcagcggtgc gcaggtggtg gtcatcgatc gcaacctcga cgtcctgcgg cgcatggatc
      421 gtctgctcgg ggcgcgcgtg atcaccgtct tttccaaccg cgacaacgtc gaacaccatg
      481 tcctgcgtgc cgacctggtg ataggtggcg tgctgattcc cggcgccgcg gcgcccaagc
      541 tggtgacccg gcagatggtc gacgccatga agccggggtc ggtgatcgtt gacgtcgcca
      601 tcgaccaggg cggctgctgc gagacggcca aggccaccac gcacgccgaa ccgacttaca
      661 tggtcggcaa ggtggtgcac tactgtgtgg ccaacatgcc cggcggcgtg ccccgcacct
      721 cgacctatgc gctcaacaat gcgacgctgc cttttgccct gcagatcgcc gacaagggct
      781 ggcggcaggc gctgcaggac aatgttcacc tgcgcaatgg actgaacgtc gctttcggca
      841 aggtcacctg ccgcgaggtg gcagaagacc tcggttacct ctaccacgat gcactgtcgg
      901 tgatcgccgg ctgagctttg gccgggcggt gggcaaggtg ggcggcgtaa agtccggatt
      961 tctcctggag tcgccagttc gcgtcgggca cccgttacga ttttgcctgg gcgccgcccg
     1021 tgcacgtgac gcatagcgcc gagagcgtcc tgacatgaag cgctcgattc acgtgctcgg
     1081 cggcgacggt ctggcaagat catgaaccct catgacggcc tctatgacga ggtggtctcc
     1141 agtgccctgt ggggtgcgct attgaagacc accggcgagg gtgagcgcac cctcgatccg
     1201 cttgccgctg aggatgttgc cgggcgctta tccgatgcac tggcccgcca actctcgcgg
     1261 atactcgagg acctgggagg caccggggaa gagagggcac accggcaatt ggaactgatc
     1321 aattcgatcc tgacggatgt tcgccatcgg cttggcggtc atggcaatca gatcgatacc
     1381 gtccacgcgc cattgcagct gctacattcc atccatcggt gtactccgcc acgcgtactg
     1441 cccgataccg gtttggccat gccttggctg tttaccgcgg gcaagggttc gccctcgcta
     1501 ctgagtgagt tacgccgcga gcttgctgcc tgcgatgaag tggatatcct ggtcagcttc
     1561 attacccatt ccggggtacg caggttgctc gatattctgc aatcggtgac ggccataggg
     1621 gctgatggcc agccgcgcag ccgcttgcgt gtattgacta ccacttacac tggcgcgacc
     1681 gagattcagg ctgtggatga gttggccaga ttgccggggt gccaggtcaa agtttctctg
     1741 gacggccgcc ggacccggtt gcatgcaaag gcctggctgt tcaggcgcca gaccggtttc
     1801 ggctcggctt atgtgggcag cgccaatctt tcaggtgcgg ccttggcggg tggacttgaa
     1861 tggacagtga agtttaccga gcgtgggcag ttggagttat tcgagcgtgc caaggcacat
     1921 tttgaaacgc tctgggacga caacgagttc caatcctatg atccggcaga tatcaggcat
     1981 cgccgagccc tgagcgacgc gctaacgcgg gaatcgggtt gcgacatcgt agcgcgaccg
     2041 accttcttcg atctggagcc gaaggcctat caacaggaga tgctcgatca gttgctgatc
     2101 gagcgtgaac gcggccggcg gcgaaatctg gttgtggcgg ccaccggtac aggcaagaca
     2161 gttgtcgccg cttttgacta tcggcgcgag tgcgaacaga agggcggaag accacgcctg
     2221 ctgttcgtcg cgcaccggtt ggaaattctc aaacaggcat tgcgcacctt ccgggaggtg
     2281 ttgcgcgacc actccttcgg cgaattgctg gtgggaggca cggagattga tcacttcgat
     2341 catcttttcg ccagcattga cagtctcgca tctcgcaatc tggtgcagcg ctgtggcgca
     2401 gactattggc atgtagtggt ggtcgacgag tgtcatcgtc tcgccgcaga gcgcttccat
     2461 aacttcgtca gcgttgtccg accacacatt ctcctgggtt tgacggcgac ccccgaacgc
     2521 agcgatgggc aacccattct gggatatttc gacaatcgac cggatggcgc tccggcagtt
     2581 gaactacggc tctggcatgc tctcgatcta caatttctga gccccttcga gtactacgct
     2641 tgcgatgacg acaccgattt ttctcaggtg ccctgggagc agccggggga actggcggcc
     2701 atagacgggt tggtcactgg caatgaggtg cgtgcccggc ttgttctcaa cgagtggcgc
     2761 cgtctggctg gcgaccctgc tcggtgccgt gccttggtgt tctgcgtttc tgtggcgcac
     2821 gcgctgtaca tgacccagaa actgaatcaa tccgggatcc gagcgctctg cgtggtggga
     2881 aacacccctg accatgagcg acgacgcgcc cccgaactgc tcgcaagtgg tcaggtctgt
     2941 gcattggtca cctgtgatct ctacaacgag ggggtggatc tgccttcggt tgatacgctg
     3001 ttgttgctgc ggcccaccca aagcccggtc ttgtttcaac agcagatcgg tcgtggcctg
     3061 cgtctggcta agggcaagga aagctgtctg gtgctcgatt ttgtggggcg ccatcggcaa
     3121 gactttcgtt tcgatcgcct gctcgctacc atcaccggcc tttcgcgtgg acagttgatc
     3181 gacgccgtcg accaagggtt tggctcgctt ccgccaggtt gccacatcca cctgcaacgt
     3241 cagacccgcg atcaggtatt gagcagtctg aaacgattgg tacagcaaaa ctggcgtcgc
     3301 ttgcgtgccg agttgcaaac ctatatggct ctcaaagggc gcgccaaggt ccgtctggcg
     3361 acttttctac gcgagcaggc tgttgatctc gaagacatct atcgaggcgc cggccgttcc
     3421 ggttggacaa acctgaagcg cgatgccgga ctattgacga ctccgacggg gccagaagac
     3481 gattattttg gcggtcgctt cgcagacctc ctgcatttcg atgacccggc acgcatcaac
     3541 ttgcttctca aagtggggga accggacgcc tcttattttc ccgaagtcaa gtctgagcag
     3601 cgactgctac agatgctggc gtatcaggtg gatgggcaac accaccggac cgggcggggt
     3661 gcagattttc tgcagcgatt gagtgtccag accgatacga gaaacgaact ggccgagatc
     3721 gcggaagtgc tgcaggggag aagcaccctc agtcatcagg cggttccggg gctggaaagc
     3781 acgcctcttt gtttgcatgc gg
//
LOCUS       DAOSIT010000189         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21166_length_3799_cov_9.687767, whole genome shotgun sequence.
ACCESSION   DAOSIT010000189 DAOSIT010000000
VERSION     DAOSIT010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21166_length_3799_cov_9.687767"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1359
                     /gene="pcnB"
                     /locus_tag="PLL14_05790"
     CDS             13..1359
                     /gene="pcnB"
                     /locus_tag="PLL14_05790"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOG03469.1"
                     /translation="MIRKFISRVLGRKGTASSGQPAVIAVVQHGLQRDQISRGCQRTI
                     ATLQENGYQAYVVGGAVRDLLAGLTPKDFDVATNATPEQVRHCFRRSRIIGRRFQIVH
                     VNMGAETIEVTTFRGHHGEQGGNTAQTDEQGRVLRDNVFGSQAEDAARRDFTVNALYY
                     DPTSETIVDYHHGVADLQQKTLRMIGDPKTRYREDPVRMLRAVRLAAKLGLLIDPAAS
                     RPIREMTDLIENVPPSRLFDEMLKLLTSGHAVRCVTRLREEGLHHGLLPLLDVILDQP
                     LGERFVMLALDATDRRVREGKPVSPGFLFATLLWNEVLVKWDLLKSRGEPVIPALFLA
                     MDEVLDVQAEKLAITRRIAGDIKDIWALQPRFEQRSGKRPYGVLQQPRFKAGYDFLLL
                     RTESGEVAPEVGTWWTDFLHASDEGRADMLLPQPPGEARKRRRRRPPSGSGASLPE"
     gene            1356..1838
                     /gene="folK"
                     /locus_tag="PLL14_05795"
     CDS             1356..1838
                     /gene="folK"
                     /locus_tag="PLL14_05795"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOG03470.1"
                     /translation="MNLAFVALGANLDDPVAQVRAAGAALANVPDSRLLRLSSLYRTA
                     PVGIRGQPDFINAVAALATRLPPSQLLDALFAVERRFGRRREFYQAPRTLDLDLLLYD
                     QLVVDSLPLSVPHPRMHLRAFVLAPLLEIAPHCRIPGRGSAVAWLPAVSMQRIEKLAA
                     "
     assembly_gap    1928..2027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2063..2875
                     /gene="panB"
                     /locus_tag="PLL14_05800"
     CDS             2063..2875
                     /gene="panB"
                     /locus_tag="PLL14_05800"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOG03471.1"
                     /translation="MSTHVATRRLTQLDLARMRANGEKIAVLTCYDASFAQVCDAAGV
                     DALLIGDSLGMVLQGHDSTLPVALSDIAYHTASVTRGCRRPLVIADMPFGTFQESPQR
                     ALRNAVTLMAAGAQMVKLEGGVDMAETTHFLTARGIPVCAHVGLTPQSVHQLGGYRVQ
                     GRDDSGATRLIADALAQQEAGAGLIVLEAIPQALAAEVTGQLTIPTIGIGASRECSGQ
                     VLVLHDMLDISPGRKARFVRNFMAGQASIAAAIAAYVTAVKDGSFPGPEHCY"
     gene            2893..3738
                     /gene="panC"
                     /locus_tag="PLL14_05805"
     CDS             2893..3738
                     /gene="panC"
                     /locus_tag="PLL14_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOG03472.1"
                     /translation="MQIHSLIPELRAALQNRGHIAIVPTMGNLHAGHIALMTQARTHG
                     DTVVATIFVNRLQFGPNDDFDRYPRTFQADCEKLAAAGVDVLFAPGEADLYPEPQQYH
                     VDPPEIQHQLDGAFRPGHFRGVATVVLKLFNIVQPQVALFGKKDYQQLLVLRNMTRQM
                     ALPIEIVGGETVRADDGLALSSRNAYLSASERVEAPRLQRLLKHIGTAICDGNRDFVG
                     LESEAVGELDRNGWKTDYVAVRRQSDLQVPQAPEEQALVVLAASRLGVARLIDNVEIN
                     AASGH"
BASE COUNT          681 a         1227 c         1119 g          672 t
ORIGIN      
        1 aaatcaccag cgatgatccg caagttcata tcccgcgtcc tcggacgaaa aggtactgct
       61 agctcagggc aaccggctgt catcgccgtc gtccagcacg ggctgcaacg ggaccagatc
      121 agtcgcggtt gtcagcgaac catcgccacg ctgcaggaga acggctacca ggcctatgtc
      181 gtcggcggtg ccgtgcgcga cctgctggcg ggactgacgc cgaaggactt cgatgtcgcc
      241 accaacgcca ccccagaaca ggttcgccat tgtttccgtc ggtcacgcat tatcggccgc
      301 cgcttccaga tcgtgcacgt caatatgggt gcggaaacca tcgaggtaac caccttccga
      361 ggccatcacg gcgaacaggg cggcaacacg gcacagactg acgagcaggg tcgtgtcctg
      421 cgcgacaatg tcttcggtag ccaggcggaa gatgcggcac gccgcgattt cacggtgaac
      481 gcgctgtatt acgacccgac ctccgaaacc atcgtcgact atcaccatgg cgtcgccgac
      541 ctgcagcaga agacgctgcg catgatcggc gacccgaaga cgcgctaccg tgaagatccg
      601 gtacgcatgc tgcgcgccgt tcgcctggcg gccaagcttg gcctgctgat cgacccggcg
      661 gccagcaggc cgatccgcga gatgaccgac ctgatcgaga atgtgccacc ctcgcgtctg
      721 ttcgacgaga tgctcaagct gctcacctcg gggcatgccg tacgctgtgt cacacggctg
      781 cgcgaggaag gactgcacca cggcctgctg ccgttgctcg acgtgattct cgatcagccg
      841 ctcggcgaac gtttcgtcat gctggcactc gacgctaccg atcgtcgcgt tcgcgaaggc
      901 aagccggtgt cacccggttt cctttttgcg acgctgctct ggaatgaggt gctggtcaag
      961 tgggatctgc tgaagagtcg cggcgaaccg gtgatccccg ccctcttcct ggcgatggac
     1021 gaagtgctcg acgtgcaggc ggaaaagctc gcgattaccc gtcgtatcgc cggtgacatc
     1081 aaggatatct gggcgttgca accacgcttc gagcagcgct ccgggaaacg gccgtacggc
     1141 gtgctgcaac agccacgctt caaggccggc tatgacttcc tgctgttgcg cacggagtcc
     1201 ggcgaagtgg ctccggaggt tggcacgtgg tggaccgatt tcctgcatgc cagcgacgag
     1261 ggtcgagcgg acatgctgtt gccgcaacct cccggcgagg caagaaagcg ccgccgtcgc
     1321 cgtccgccat cgggcagcgg cgcgagcctc cctgaatgaa cctggccttt gtcgcgctgg
     1381 gcgccaatct ggacgatccg gtggcccagg tccgggcggc cggtgctgcc ctggccaacg
     1441 tccccgactc ccgcctgctg cgcctctcct cgctgtaccg caccgctccg gtcggaatcc
     1501 gtggtcaacc cgatttcatc aatgccgtgg ccgcactggc aacccgactt ccaccgtcgc
     1561 aactgctcga tgccctgttc gcggtcgaac gccgctttgg tcggcgtcgc gagttttatc
     1621 aggcgccgcg taccctcgac ctcgacctgc tgctctacga tcaactcgtc gtcgatagcc
     1681 tgccgttgag cgtaccgcac ccgcgcatgc acttgcgcgc cttcgtgctt gcgcccttgc
     1741 tcgagattgc gccgcactgc cgcattccag gccgtggtag cgccgtcgcc tggctgccgg
     1801 cagtcagcat gcagcgcatc gaaaaactgg cggcctaata gtacggacgg taacggggtg
     1861 ctccatagcc acgaccatag ccaccaccat aaccgtagta gtccggccgt cccgggacca
     1921 cgaccagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat ccgcgaccac
     2041 tacaacccgg gagagtaaac ccatgtccac ccacgttgcg acgcgccgat tgacgcagct
     2101 cgatcttgcc aggatgcgtg ccaatggtga aaagatcgcc gttctgacct gctacgatgc
     2161 gagcttcgca caggtctgcg atgcggccgg cgtcgacgcc ctgctgatcg gcgattcgct
     2221 cgggatggtc ctgcagggcc acgactcgac attgccggtc gcattgtccg acatcgccta
     2281 ccacacggcg agcgtcactc gcgggtgcag gcgtccgctg gtgatcgccg acatgccctt
     2341 cggcactttc caggagagtc cccagcgtgc cctgcgtaat gccgttacac tgatggccgc
     2401 cggggcacag atggtgaaac tcgaaggcgg cgtcgatatg gccgaaacga cccacttcct
     2461 gaccgcacgt ggaatcccgg tgtgtgcgca cgtcgggctg accccgcagt cggtgcatca
     2521 gctcggaggc taccgtgttc agggtcggga tgactcgggg gccacccgtc tgatcgccga
     2581 tgcacttgcg cagcaggaag cgggcgcggg gctgatcgtc ctcgaagcca tcccgcaggc
     2641 attggcggcc gaagtgaccg gccagctcac gataccgacg atcggtatcg gtgccagccg
     2701 ggaatgctcg ggccaggtgc ttgttctgca cgacatgctg gatatctccc ccggacggaa
     2761 ggcacgcttc gtcaggaatt tcatggccgg tcaggcgtcc atcgccgcag ccatcgctgc
     2821 ttacgtcact gcggtcaagg acggcagctt cccgggcccg gaacactgct actgaaagag
     2881 gttggccaga ccatgcagat ccattcgctg atccccgaac tgcgcgctgc gctacagaac
     2941 cgtggccata tcgccatcgt gccaacgatg ggtaatctgc acgccggcca tatcgcgctg
     3001 atgacccagg cccgtaccca cggcgatacg gtggtggcca ccatcttcgt caatcgactg
     3061 cagttcgggc ccaatgacga cttcgaccgt tatccgcgca ccttccaggc cgattgcgag
     3121 aagcttgccg ccgccggagt cgacgtcctg ttcgcacccg gtgaggccga tctttatccc
     3181 gagccacagc agtaccatgt cgacccgccg gagattcagc atcaactcga cggcgcgttt
     3241 cgcccgggtc acttccgcgg tgtcgccacc gtcgtcctca agctgttcaa cattgtccaa
     3301 ccgcaggtgg cgttgttcgg caagaaggac tatcagcaac tgctggtctt gcgcaacatg
     3361 acgcgccaga tggccttgcc gatcgagatc gtcggcggcg aaacggtgcg tgccgacgac
     3421 ggcctggcac tgtcttcgcg caacgcctat ctgtcggcgt ctgaacgcgt cgaggctccc
     3481 cgcctgcaac gcctcctgaa acacatcgga acggcaatct gcgacggtaa ccgggatttt
     3541 gtcggcctcg aaagcgaagc cgttggcgaa ctcgaccgca acggctggaa gacggactat
     3601 gttgcagtgc ggcgtcagtc agacctgcag gtgccgcaag cccccgaaga acaggcgctg
     3661 gtggtccttg cggcgagccg cctgggcgtc gcgcgactca tcgacaatgt cgaaatcaac
     3721 gctgcgagcg ggcattgaca ggtggccatt agccgctaca attcgcttcc ccaaactcta
     3781 aaccggtgaa aatccatgc
//
LOCUS       DAOSIT010000190         3786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21249_length_3786_cov_9.931386, whole genome shotgun sequence.
ACCESSION   DAOSIT010000190 DAOSIT010000000
VERSION     DAOSIT010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21249_length_3786_cov_9.931386"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLL14_05810"
     CDS             complement(<1..1190)
                     /locus_tag="PLL14_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOG03473.1"
                     /translation="MEALKSGTDVLFILLGGIMVLAMHSGFAFLELGTVRKKSQVNAL
                     VKILTDFSVSTIAYFFIGYSLAYGVNFFSGAETLLQKNGFELTRFFFLLTFAAAIPAI
                     VSGGIAERARFYPQLAATFLIVGFIYPFFEGIAWNQAYGIQAWLKATYGEEFHDFAGS
                     VVVHAMGGWIALPAVVLLGARHGRYTKEGRLSAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQTIDKISGLVAMNSLMAMVGGTLVALVAGRNDPGFVHNGPLAGLVAVCAGSDVMH
                     PIGALFVGGVAGGLFVVMFTLTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGSRAMA
                     GVGGVAFMPQLLMTGLAVAVALVGSCLVYGLLRVAFGLRLDQEQEYNGADLSIHKITA
                     EPER"
     assembly_gap    1228..1327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1328..2046)
                     /locus_tag="PLL14_05815"
     CDS             complement(<1328..2046)
                     /locus_tag="PLL14_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOG03474.1"
                     /translation="MHKSQPRRLLLILVTLLAALLSACATTREGASGACPAPASCPVC
                     PQCAAGAAVPPATASKPLQAARWSDLPGWSEDDLAAAWPAFLQSCRALSSRPQWPQWQ
                     AVCAEARTLNGPTTAAVRRFFESRFQPWLMTNPDGTTKGLVTGYYEPLLRGSRTRGRP
                     YLSPVLGVPADLLTIDLGTVLPDLKNLRLRGRLQGGKVVPYFSRAEISSHEQDYADRT
                     LLYVDDPVELFFLQVQGSGRV"
     gene            2095..3057
                     /locus_tag="PLL14_05820"
     CDS             2095..3057
                     /locus_tag="PLL14_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03475.1"
                     /translation="MIDSFRQLARFLVRAEQFLDRLEPLLPPASAGPDWAAACAFRWR
                     TQRGTGYLQPITRLPGIRLSDLQDIDEQKARIDANTRHFVAGLPANNVLLTGARGSGK
                     SSLIKALLNEYAPRGLRVIEVEKGDLIDLPDIVEMIDGRPERFIIFCDDLSFDAGDAR
                     YKALKVVLDGSFAAAPENVLVYATSNRRHLMPEYFAENLDHQHQGEEIHPGEAIEEKI
                     SLSERFGLWISFYPFDQEAYLKIVAHWLGAFGCHQDEIVSAERDALNWALERGSRSGR
                     VAWQFARDWAGRQRVAPSRTKRRRTAALPVAVPPSARKRSGDGA"
     gene            3095..>3786
                     /locus_tag="PLL14_05825"
     CDS             3095..>3786
                     /locus_tag="PLL14_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG03476.1"
                     /translation="MKQITEVAAAVMLRGDPAQPEFLLAQRPPGKVYAGYWEFPGGKV
                     EPGETAHEALIRELQEELGVTVDRAWPWLCREFSYPHATVRLRFFRVSSWQGELTAVE
                     HSALVWLKAGQPADVSPILPANGPILGALALAPVYALTNAEEYGIDAELLRLERALCN
                     GLRLLQVRDKGLAPARREAFAEAVLALARNHDGTTVLINDDPRLARNVGAAGVHLSSR
                     LLWQTDRRPDFER"
BASE COUNT          724 a         1173 c         1177 g          612 t
ORIGIN      
        1 tcgcgctcgg gttcggccgt gatcttgtgg atcgaaagat ccgcgccgtt gtattcctgc
       61 tcctggtcga ggcgtaatcc aaaggccact cgcagcaggc catagaccag gcaactgccg
      121 accagggcaa cggcaaccgc cagtccggtc atcagcaatt gcggcatgaa cgccacgccg
      181 ccaacaccgg ccatggcgcg actgccgaag atgccggcgg caatgccacc ccaggcgccg
      241 caaagaccat gcaggggcca gacgccgagg acgtcgtcga tcttccagcg gttctgggtc
      301 agcgtgaaca tgacgacgaa caggccgccg gcgacaccac caacgaacag ggcgccgatc
      361 ggatgcatga catccgagcc ggcgcaaacc gccaccagac cggcaagcgg gccgttgtgc
      421 acgaagccgg gatcgttcct gcccgccacc agagccacca gggtaccccc aaccatggcc
      481 atcagcgagt tcatggcgac caggccggag atcttgtcga tcgtctgcgc gctcatgacg
      541 ttgaagccga accagccaac cgtcagaatc caggctccga gcgcaagaaa cggtatcgat
      601 gacggggggt gcgccgacag gcggccttcc ttcgtgtagc gcccgtgacg tgccccgagc
      661 aggacaacag ccgggagggc gatccagccg cccatcgcat gcacgacgac cgatccggcg
      721 aaatcgtgga attcttcacc gtaagtcgcc ttcagccagg cctggatgcc gtacgcctga
      781 ttccaggcga tgccttcgaa gaagggatag atgaagccga cgatcagaaa ggttgccgca
      841 agttgtggat agaagcgcgc gcgttcggca atgccgccgg atacgatggc gggaatcgcc
      901 gccgcgaaag tgagcaggaa aaagaagcgc gtcagctcga aaccgttctt ctgcaacagg
      961 gtctcggcgc cggagaagaa gttgacgccg taggcaagac tgtagccgat gaagaaatag
     1021 gcgatcgtcg ataccgagaa atcggtcagg atcttcacca gcgcgttgac ctggctcttc
     1081 ttgcgtacgg tgccgagttc gagaaaggca aagccggaat gcatcgccaa caccatgatg
     1141 ccgccaagca gaatgaacag aacgtcggta ccagacttga gtgcttccat gactcctcct
     1201 gaaattcggg tcgaagccat caagcaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnctg acgcgccccg aaccctgcac ctgcagaaag aacagttcaa ccggatcgtc
     1381 gacatagagc agggtccggt cggcgtagtc ctgctcatga ctgctgattt ccgcacgcga
     1441 aaaataggga acgaccttgc cgccctgcag gcggccgcgc agacgcaggt ttttcagatc
     1501 cgggagtacc gtaccgagat cgatggtcaa cagatccgcc ggtacaccga gcacgggcga
     1561 cagataaggc ctgccgcggg tccgcgagcc gcgcaacagc ggctcgtagt agccggtcac
     1621 cagccccttc gtcgtgccat ccggattggt catcaaccag ggctggaaac gcgactcgaa
     1681 gaaacgccgc acggcggccg ttgtcggccc gttcagagtc ctggcttcgg cacagacggc
     1741 ctgccactgc ggccactgtg ggcggctgga gagcgcacga cacgactgca ggaatgccgg
     1801 ccaggccgcc gcgagatcat cttcactcca acccggcaga tcgctccagc gcgccgcctg
     1861 gagcggtttg ctggccgtcg ctggtggtac ggccgcgccg gcggcgcatt gcggacagac
     1921 cggacagcta gccggggcgg gacatgcgcc acttgcccct tctctggtgg tagcacaggc
     1981 gctgagcagc gccgccagca gcgtgacaag aatcaggagc aagcgccgtg gttggctttt
     2041 atgcatggtt tggctagagt gtcgggttgg tcaacctgcg aagtatactg tccgatgatc
     2101 gattccttcc ggcaacttgc gcgttttctc gtccgcgccg agcaattcct cgatcgtctc
     2161 gagcccttgc tgccacccgc atcggccggc ccggactggg ctgcggcctg cgcttttcgc
     2221 tggcgtacgc agcgcgggac gggttatctg cagccgatca ctcgcctgcc cggcatccgg
     2281 ctcagcgacc tgcaggacat cgatgaacag aaggcgagga tcgacgccaa tacgcgtcat
     2341 ttcgtcgccg gcctgccggc caacaatgtg ctgctcacgg gcgcccgcgg ctcgggcaag
     2401 tcttcgctga tcaaggccct gctcaacgag tacgcaccgc ggggcctgcg cgtcatcgag
     2461 gtcgagaagg gtgacctgat cgacctgccg gacatcgtcg aaatgatcga cggacgcccg
     2521 gaacgcttta tcatcttctg tgacgatctt tccttcgatg ccggcgacgc gcgctacaag
     2581 gcgctgaagg tcgtgctcga cgggtcgttc gcggcagcgc cggaaaatgt gctggtctac
     2641 gccacgtcga accgccgcca tctgatgccc gagtattttg ccgagaacct cgaccaccag
     2701 caccagggtg aagaaatcca tcccggtgag gccatcgagg aaaagatctc gctgtcggag
     2761 cgtttcggtc tgtggatttc tttctatccc ttcgatcagg aggcctatct caagatcgtt
     2821 gcgcactggc tgggcgcctt cggctgtcac caggacgaga tcgtgtctgc cgaacgcgac
     2881 gcactcaact gggcacttga acgcggttcg cgcagcgggc gcgttgcctg gcagttcgcc
     2941 agggattggg ccggtcggca gcgggtggcg ccgagtcgca cgaaacggcg acgtaccgca
     3001 gcgttgcccg tcgccgtacc gccgtctgcc cgaaaacgct cgggggacgg cgcgtgagcc
     3061 agatccggcg gggacgcgtg gcgccaggcg accgatgaaa cagattaccg aagttgcagc
     3121 ggcggtcatg ctgcgcggtg acccggcaca acccgagttc ctgctcgcgc aacggcctcc
     3181 cggaaaggtg tacgccggct attgggaatt ccccggtggc aaggtcgagc ctggcgagac
     3241 agcgcacgag gcactgattc gcgaactgca ggaagagttg ggggtcaccg tcgaccgtgc
     3301 ctggccgtgg ctatgccgcg agttcagcta tccgcacgcg accgtccgcc tgcgtttttt
     3361 tcgcgtcagt tcatggcagg gcgaactgac cgcggtcgaa cacagcgccc tggtatggct
     3421 gaaagctggc cagcccgcgg acgtcagccc gatcctgccg gccaatggtc cgatcctcgg
     3481 cgcgcttgcg ctcgcgccgg tctatgcgct gaccaatgcc gaagaatacg gcatcgacgc
     3541 cgagctgctg cggctggaga gggcgctgtg caacggcctg cgcctgctgc aggtacgcga
     3601 caaggggctg gcgcccgctc ggcgcgaagc ctttgccgag gcggtgctgg cgctggccag
     3661 aaaccacgac gggaccaccg tcctgatcaa cgatgaccca cgcctagccc gtaatgtcgg
     3721 cgcggctggc gtgcatctct cgtcgagact gctctggcag accgatcggc ggccggattt
     3781 cgagcg
//
LOCUS       DAOSIT010000191         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21359_length_3772_cov_8.941620, whole genome shotgun sequence.
ACCESSION   DAOSIT010000191 DAOSIT010000000
VERSION     DAOSIT010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21359_length_3772_cov_8.941620"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLL14_05830"
     CDS             <1..390
                     /locus_tag="PLL14_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOG03477.1"
                     /translation="DGEVAVEKVKPAKKKKVDRGAPPVYVAIDAFTVNLVPERGDQFL
                     QLSLSVEVDDVQIGDQMKQYMPKLRNDLTLLLSSKKASDLVTTEGKELLAQEIREQIN
                     NILDPASKGKKRDGPIKDVLFTSFIIQ"
     gene            397..1401
                     /gene="fliM"
                     /locus_tag="PLL14_05835"
     CDS             397..1401
                     /gene="fliM"
                     /locus_tag="PLL14_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOG03478.1"
                     /translation="MSADFLSQDEVDALLKGVSGEADETEAVEDTDDGPRAYNLGTQE
                     RIVRGRMPTLELVNERFARYLRIGLFNYMHRTTEISVGSIRVQKYSEFTRNLVVPTNL
                     NLVAARPLRGTALFVFDPSLVFLVVDNMFGGDGRFHTRVEGRDFTPTEQRIIQGLLKI
                     VFAEYSRSWKSVYDLTFEYLRSEMNSQFANIATPSEIVVSTTFSLEFGGSSADMHICF
                     PYSMLEPIRDLLYSAMQSDQLTTDRRWIIMLRKQLKNAEVEISAKLATTTVSLRQILT
                     MKVGDIIPLDVPDKVVALVDDVPVMECHYGQQGGQYALKIDRFIASENADVPLGEING
                     "
     gene            1394..1825
                     /gene="fliN"
                     /locus_tag="PLL14_05840"
     CDS             1394..1825
                     /gene="fliN"
                     /locus_tag="PLL14_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOG03479.1"
                     /translation="MAKETDAAPASDDAGLDDDWAAAMAEQAMAEGTPSSSAQAANIF
                     PSFDTTGNKSSLMNELDMILDIPVQISVELGRTRITIKNLLQLAHGSVVELDALAGEP
                     LNVFVNGTLIAQGEVVVVNDKFGIRLTDIVTPSERARKIGH"
     gene            1933..2385
                     /gene="fliO"
                     /locus_tag="PLL14_05845"
     CDS             1933..2385
                     /gene="fliO"
                     /locus_tag="PLL14_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOG03480.1"
                     /translation="MIRHAPSILSSGTGKACRLLLAMLPGGAFAQAAATETPGVSAST
                     LLQTVLGLALVIGILLLAAYFLRRLNGGRGFGSNGPLRVVGGLMIGPRERIVLVEIDD
                     TWIVVGLVPGQIRTLHTLPKGELKPGSDGERHFAQWLKQITERKNEGS"
     gene            2372..3124
                     /gene="fliP"
                     /locus_tag="PLL14_05850"
     CDS             2372..3124
                     /gene="fliP"
                     /locus_tag="PLL14_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768226.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOG03481.1"
                     /translation="MKAAKAASFLVLCALLCPLAAWAQTGGLPMVTSTPGAAGSQTYS
                     LSIQSLLTLTALTFIPAALLMMTSFTRIIIVLSLLRAALGTQNSPPNQVLLGLSLFLT
                     FFIMSPVLDTIYTEAYLPLSEDRITYIQAVERGAVPLRKFMLKQTREADIALFARMAA
                     SPKFQSTDDIPMRLLIPAFVTSELKTAFQIGFIIFIPFLIVDMVVASALMSMGMMMMS
                     PVMVALPFKLMLFVLVDGWNLLLGSLVASFAA"
     gene            3121..3390
                     /gene="fliQ"
                     /locus_tag="PLL14_05855"
     CDS             3121..3390
                     /gene="fliQ"
                     /locus_tag="PLL14_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOG03482.1"
                     /translation="MTPEIVMDIGRQAVEMTLLLSAPLLLAALVIGLIVSVFQAATQI
                     NEQTLAFIPKLVGMFLVLLLAGPWMLQLMVEYVRRLFESIPLLVG"
     gene            3398..>3772
                     /locus_tag="PLL14_05860"
     CDS             3398..>3772
                     /locus_tag="PLL14_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOG03483.1"
                     /translation="MINVTAAEINAWVVAFLFPLARVLALLSAAPPFNNPALPTRIRL
                     ILGLAITFAIAPGLPPMPSLDPGSGAGLLLLAQQLVVGLAMGFAMRLVFTAVDMAGNL
                     ISLQMGLGFASAYDPQTAGQTAV"
BASE COUNT          752 a         1162 c         1068 g          790 t
ORIGIN      
        1 gacggcgagg tcgccgtgga aaaggtcaag ccggccaaga agaagaaggt ggaccgggga
       61 gcgccgccgg tctatgtcgc aattgatgcg ttcacggtca atctcgtacc ggaacgcggc
      121 gaccagttcc tgcagttgtc gctttccgtc gaggtcgatg atgtacagat cggcgaccag
      181 atgaaacagt acatgcccaa gctgcgcaac gacctgactt tgctcctctc cagcaagaag
      241 gcttccgatc tggtcaccac cgagggcaag gaactcctgg cacaggagat cagggaacag
      301 atcaacaaca ttctcgatcc ggccagcaag ggaaaaaaac gtgacggtcc catcaaggac
      361 gtgctgttca cctcgttcat catccaatga actgcgatga gcgccgattt cctctcccag
      421 gatgaagtcg acgccctgct caagggcgtc agtggtgaag ccgacgaaac tgaagccgtt
      481 gaggataccg acgatggccc gcgggcctat aacctcggaa cgcaggagcg catcgtccgc
      541 gggcgcatgc ccacgctcga actggtcaat gagcgctttg cccgctatct gcgcataggt
      601 ctcttcaatt acatgcaccg caccaccgag atctcggtgg gctcgatacg agtgcagaaa
      661 tacagcgagt tcacccgcaa cctggtggtc ccgacgaacc tgaatctggt cgccgcgcgg
      721 ccattgcgtg gcacggcatt attcgtcttc gaccccagcc tggtatttct ggttgtggac
      781 aacatgttcg gaggtgacgg tcgcttccac acccgtgttg agggccgcga ttttacgcca
      841 acagaacaac gcatcatcca gggactgctc aagattgttt tcgcggaata ctcgcgatca
      901 tggaagtcgg tctacgacct gactttcgaa tatctgcgct cggaaatgaa ttcccaattc
      961 gcgaatattg cgacgccttc cgaaatcgtc gtgtcgacga ccttctcgct ggaattcggt
     1021 ggctccagcg ccgatatgca catctgcttt ccgtattcga tgctcgagcc gattcgtgac
     1081 ctgctctata gcgccatgca gagcgaccaa ctgaccaccg accgacgctg gatcatcatg
     1141 ttgcgcaagc aactgaaaaa cgccgaggtt gaaatcagcg cgaaactggc gaccacgaca
     1201 gtgagtctgc gccagattct aacgatgaaa gtcggcgaca tcattcccct cgacgtcccg
     1261 gacaaagtcg tcgccctggt cgacgatgtt cccgtaatgg agtgtcacta cggccagcaa
     1321 ggtgggcaat acgcgctcaa gatcgaccgc ttcatcgctt cagagaacgc agacgttcct
     1381 ctgggagaaa tcaatggcta aggaaacaga cgcggcgccg gcaagcgacg acgctggact
     1441 cgacgatgac tgggctgcgg cgatggccga acaggcgatg gctgaaggca cgccgtccag
     1501 cagcgcacaa gccgccaata ttttcccgag tttcgatacc accggcaaca agagttcgct
     1561 gatgaatgaa ctcgacatga tcctggacat tccggtccag atctcggtcg aactcgggcg
     1621 cacccggatc accatcaaga acctcctgca actggcgcac ggctcggtcg tcgaactgga
     1681 cgcgctggcc ggggaaccgc tgaacgtttt tgtcaatggc acgctgatcg cccaaggtga
     1741 ggtcgtggtg gtcaatgaca agttcggcat tcgcctgaca gatatcgtga ccccttccga
     1801 aagagcacgc aagatcgggc actgagcacg gcagccttcc acacctgcac tcacgctgtt
     1861 cgtgcacaac gttttgacct tcgccatgct tgctttaaga tagtcgtcag catccacgtc
     1921 cggccaaccc tcttgattcg acacgccccc tccatcctct ccagcggcac cggtaaagca
     1981 tgccgtttgc ttctcgcgat gttgccgggt ggtgcttttg cgcaggcagc ggccaccgaa
     2041 accccggggg tttcagcaag cacactgcta cagaccgttc ttgggctggc attggtcatc
     2101 ggcattctgt tgcttgcagc ctattttctg cgccggctca atggtggtcg cggctttggc
     2161 agcaacggcc ccttacgcgt cgtgggtggc ctgatgattg gcccgcggga gcgcatcgtg
     2221 ctcgtcgaaa tcgatgatac gtggatcgtt gtgggccttg tgccaggcca gatccgcacc
     2281 ctgcacacgc tgccaaaagg agaactgaaa cccgggagtg atggtgaacg acatttcgca
     2341 caatggctga agcagattac cgagcgcaag aatgaaggca gctaaggccg ccagcttcct
     2401 cgttctgtgc gcattgctct gcccgttggc ggcctgggcg caaacgggtg ggttaccgat
     2461 ggtcaccagt actcctggcg cggccggaag ccagacctac agcttgtcga tccagagcct
     2521 gctgacgctg accgcgctga cgttcatccc ggcagcgctg ttgatgatga ccagcttcac
     2581 gcgcatcatc atcgttctgt cactgctacg ggccgcgctg ggcacgcaga actcgccacc
     2641 gaaccaggta ctgctgggcc tctcgctgtt cctcacgttc ttcatcatgt cgccggtgct
     2701 cgacaccatc tacaccgagg cttatctgcc cttgtcggag gaccggatca cctacatcca
     2761 ggctgtcgag cgaggtgccg tgccgctgcg caagttcatg ctcaagcaga cacgcgaggc
     2821 cgacatcgcc ttgttcgccc gcatggctgc atccccgaaa tttcagtcta ccgatgacat
     2881 tcccatgcgc ctgctgatcc cggctttcgt caccagcgaa ttgaagacgg cctttcagat
     2941 tggcttcatc atcttcatcc ccttcctgat cgtggacatg gtggtggcca gcgcgctgat
     3001 gtcgatggga atgatgatga tgtcgccggt catggtggcc ctgcccttca agctgatgct
     3061 cttcgtgctt gtcgatggct ggaatttgct gcttggatcg ctggtcgcga gcttcgccgc
     3121 gtgactcccg aaatcgtcat ggatatcggg cggcaagcag ttgaaatgac cctgctgttg
     3181 tccgccccac tgctgctggc agcactggtg atcggcctga tcgtcagcgt ctttcaggcg
     3241 gcgacccaga tcaacgaaca gaccctcgcc ttcatcccca agctggtagg catgttcctg
     3301 gtactccttc tggccggccc ctggatgctg caactcatgg tcgagtacgt ccgtcggctg
     3361 ttcgagagca ttccgctgct cgtcggctga gtgcgcgatg atcaacgtca ccgccgccga
     3421 gatcaacgcc tgggtagtgg ccttcctgtt ccctctggca cgcgtcctcg ccctgctgag
     3481 cgcggctcca ccattcaaca atccagcact gccgactcgc atccggctga tactcggcct
     3541 ggcgatcacc ttcgccatcg ccccgggact gccaccgatg ccgtcgcttg acccggggtc
     3601 aggggccggg ctcctgttgc tcgcccaaca gcttgtcgtc ggattggcca tgggttttgc
     3661 catgcgcctg gtgttcaccg cggtcgacat ggcaggcaat ctcatcagcc tgcagatggg
     3721 gctggggttt gccagcgctt acgacccgca gaccgccggt cagacggcgg tg
//
LOCUS       DAOSIT010000192         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21383_length_3768_cov_8.667116, whole genome shotgun sequence.
ACCESSION   DAOSIT010000192 DAOSIT010000000
VERSION     DAOSIT010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21383_length_3768_cov_8.667116"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..602
                     /gene="tatB"
                     /locus_tag="PLL14_05865"
     CDS             177..602
                     /gene="tatB"
                     /locus_tag="PLL14_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOG03484.1"
                     /translation="MFDIGFSEMLVIAIVALVVIGPERLPRVARTAGHLLGRLQRYVS
                     DVKSDINREMQLEELKKLQSEIRESARSVEQSLTTEMQAARQALDQIPATVANDISAP
                     AALEYPTIAETPSTATGNPQPPAPTGPTLADAAQPTRTR"
     gene            608..>1146
                     /gene="tatC"
                     /locus_tag="PLL14_05870"
     CDS             608..>1146
                     /gene="tatC"
                     /locus_tag="PLL14_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOG03485.1"
                     /translation="MNLPEDSFLSHLVELRDRLIRALIAIGIVFICLFPWAKELYALL
                     AQPLLATLPQGGQMIATDVIGVFLVPMKVALMVAFLIALPYVLYQVWAFVAPGLYAHE
                     KRLALPLVAASVLLFFLGMAFAYFLVFPTVFGFMATVAPEGVAWMTDIEKYLSFVLTT
                     FIAFGVTFEVPVVVVVLVQA"
     assembly_gap    1147..1246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1247..1889)
                     /locus_tag="PLL14_05875"
     CDS             complement(<1247..1889)
                     /locus_tag="PLL14_05875"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOG03486.1"
                     /translation="MCQLLAMNCNVPTDICFSFAGFQARGGATDVHADGWGIAFFEGR
                     GTRVFLDPQPSCVSPVAALVRSYPIHSKNVIAHIRKATQGGVALENTHPFMRELWGRY
                     WIFAHNGNLPDFSPELDGSFTPVGETDSERAFCWLLQNLRQRFGSRAPTQVSLFDALH
                     QMTIELGARGACNYLLSNGDCLFAHCSTQLSYIIRQAPFSVAHLRDQDLSIDFS"
     gene            complement(1904..3079)
                     /locus_tag="PLL14_05880"
     CDS             complement(1904..3079)
                     /locus_tag="PLL14_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG03487.1"
                     /translation="MRRLWLIFAQTVTVGLAILFVVSTLKPEWLGKRPPAGSSVVALQ
                     ESAVAAAPAIAATSFRDAAKKALPSVVHIFTSQKIKTRRNPLFDDPVFRHFFGDSAEG
                     EGPRTSGLGSGVIVSQNGYILTNFHVVETADQIEIALSDGRNLNARVVGSDPESDLAV
                     LQITEQNGKELVLPAITLGKMENVVVGDVTLAIGNPFGVGQTVTMGIISALGRSHLGI
                     NTFENFIQTDAAINPGNSGGALVDSQGNLIGINTAIYSRSGGSLGIGFAIPISIARNV
                     MEQIIQSGSVTRGWIGVEAQEISIDLAESFGLPDTNGALIAGVLRGSPADAGGIKPGD
                     ILLAVDGTAVKDPQGMLDLIASQKPGETVAFKLRRQNNVLETTVRIGKRAPQRRERD"
     gene            3101..>3768
                     /locus_tag="PLL14_05885"
     CDS             3101..>3768
                     /locus_tag="PLL14_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG03488.1"
                     /translation="MKREELSRYLDKLLEAGRFRDYCPNGLQVEGRPEVQRLLAGVTA
                     NQSLLDAAVARGADAVLVHHGWFWRGDDLRVTGLRKTRLQTLLRHDINLIAYHLPLDA
                     HPEFGNNAQLGKRLGWIADDHFGEQELGWQGHLESPCSVSAIADRVGSELQHRPLVIA
                     GDDRMVCRVAWCSGAAQGLFEQAITLGVDLYLSGEISEPTVHLARESGVAYIAAGHHA
                     SERYG"
BASE COUNT          725 a         1149 c         1093 g          701 t
ORIGIN      
        1 ggcaccggtc agaccatcga gggggaaatc agggaaaaga cccgcagcta gttatggcgc
       61 cagtcacgtg atcacacaca ccaggaagag gcgtctgtct cgatcttggg agacgaaccg
      121 cccacccgct cggcagacga ccccgcatcc cttgctctac aatcaggcgg cagtcaatgt
      181 ttgatatcgg cttttccgag atgctggtca tcgccatcgt cgcactggtg gtgatcggtc
      241 ccgaacggct acccagggta gcgcggacgg ccgggcatct gctcggccgt ctgcaacgtt
      301 acgtcagtga cgtgaagtcg gatatcaacc gagaaatgca actcgaagaa ctgaagaaac
      361 tgcagtccga gatccgggaa tccgcgcgta gcgttgagca gagtctgacc acagaaatgc
      421 aggcagccag acaggctctc gaccagatcc cggcaacggt cgccaacgat atttctgctc
      481 cggcggcgct cgaatacccg acaatcgcag agacgccatc gactgcaacg ggcaaccccc
      541 aaccaccagc accgactggc cccacgctag ctgacgctgc gcaacccacc cgcacgcgct
      601 gatcgcgatg aacctccccg aagactcctt tctctcgcac ctggtcgagt tgcgtgaccg
      661 cctgatacgg gctctgatcg cgataggcat cgtcttcatt tgcctcttcc cctgggccaa
      721 ggaactttat gccttgttgg cgcagccctt gctggcgaca ctgccgcaag gtggccagat
      781 gatcgccacc gacgtgatcg gagtttttct ggtgccgatg aaagtggcgc tgatggtggc
      841 attcctgatc gccctgccct acgtgcttta ccaggtctgg gcttttgtcg cccccggcct
      901 ctatgcgcac gagaaacggc tggcactgcc gctggttgcc gcgagcgtgc tgctgttctt
      961 tctcggcatg gctttcgcct attttctggt ctttcccacg gttttcggct tcatggccac
     1021 ggtggcgcca gaaggtgtcg cgtggatgac cgacatcgag aagtacctct ctttcgtact
     1081 gaccaccttc atcgccttcg gggtgacttt cgaggtcccg gtggtggtgg tcgttctggt
     1141 ccaggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct gaaatcgatg
     1261 ctcagatcct gatcgcgcag gtgcgcaacg ctgaaaggtg cttgtcggat gatgtaactg
     1321 agctgggtgg agcagtgggc gaacagacag tcgccattcg acagcagata gttgcaagca
     1381 ccacgcgctc caagctcgat tgtcatttga tgaagtgcat caaacaggct gacctgcgtc
     1441 ggcgcccggg agccaaagcg ttggcgcaga ttctgcagca gccagcaaaa cgcgcgctcg
     1501 ctgtcggttt caccaaccgg cgtgaaactg ccgtcgagtt cgggcgagaa atcgggcagg
     1561 ttgccgttgt gggcaaaaat ccagtaacgc ccccacagtt cgcgcatgaa ggggtgcgtg
     1621 ttctccagcg caaccccccc ctgcgtcgcc ttgcggatat gggcaatgac gttcttcgaa
     1681 tggatcggat agctgcgcac cagtgcggct accggcgaaa cgcaggaagg ctgcggatcg
     1741 aggaacacgc gcgtgccacg accttcaaag aaggcgattc cccaaccatc cgcgtgcaca
     1801 tcggtggcgc cgccacgcgc ctgaaagccg gcgaaggaaa agcagatatc cgtcggcaca
     1861 ttgcagttca tggccagcaa ctgacacatc gatcttgcct cggctagtcg cgctcgcggc
     1921 gctgcggcgc gcgcttgccg atccggacgg tggtttccaa cacgttgttc tggcggcgca
     1981 acttgaaggc aaccgtctca cccggcttct gactggcgat caggtcgagc atgccttgcg
     2041 gatccttgac cgccgtgcca tcgacagcga gcaggatgtc gcccggcttg atcccgcccg
     2101 catccgccgg gctgccgcgc aggacgccgg caatcaaggc gccgttggta tccggcagtc
     2161 caaaggactc cgccagatcg atgctgatct cctgtgcttc gacgccgatc cagccacgcg
     2221 tcacgctgcc actctggatg atctgctcca tgacattcct cgcaatcgag attggaatcg
     2281 cgaaaccgat cccgagcgag ccgcctgaac gcgaataaat ggccgtgttg atgccgatca
     2341 gattgccctg gctgtctacc agtgcccccc ccgaattgcc cgggttgatc gccgcatcgg
     2401 tctggatgaa gttctcgaag gtgttgattc ccagatgcga gcgacccagg gccgaaatga
     2461 ttcccatggt caccgtctga ccaaccccga aaggattgcc gatggccagc gtgacgtcac
     2521 ccaccacgac attctccatt ttcccgagcg tgattgccgg caggaccagt tccttgccgt
     2581 tctgctcggt gatctggagt acggcgaggt cggactccgg atcactcccg acaacacggg
     2641 cattgaggtt gcggccgtcg ctgagggcaa tctcgatctg gtcggcagtt tcgacaacat
     2701 gaaagttcgt gagaatatag ccgttctggc tgacaatgac ccccgaaccg agtccggagg
     2761 tcctcggacc ctccccttcg gcgctgtcac cgaagaagtg acggaatacc ggatcgtcaa
     2821 atagcggatt acgacgcgtc ttgatctttt gtgaagtaaa aatgtgcact accgagggca
     2881 gggctttttt cgccgcgtcg cgaaaggatg tggcagcaat ggctggcgcc gccgccaccg
     2941 ccgactcctg caaggcgacg acgctgctgc cggccggagg gcgcttgccc agccactccg
     3001 gcttgagcgt gctcaccacg aagaggatgg cgagaccaac cgtcaccgtt tgtgcaaaaa
     3061 tcagccagag cctgcgcatg ggcaaaaccg gagccagtga atgaaacgcg aagaattgtc
     3121 acgctacctg gacaagttgc tggaggcagg gcgcttccgg gattattgcc caaacggcct
     3181 gcaagtggaa ggacggcctg aggtccagcg cctgctcgcc ggcgtgaccg ccaaccaaag
     3241 cctgcttgat gccgcggtcg cccgcggtgc cgacgcagtc ctggtccatc acggctggtt
     3301 ctggcgcggc gacgatttgc gggtcactgg cctgcgcaag acgcgcctgc aaacgctgct
     3361 gaggcacgac atcaacctca tcgcctatca cctgccgctc gatgcccatc ccgagttcgg
     3421 caataatgcg caactgggta aacgactcgg ctggattgcc gacgaccact tcggcgaaca
     3481 ggaactcggc tggcagggac acctggaaag cccgtgcagc gtttcggcca tcgccgatcg
     3541 cgtcggcagc gagttgcaac accgaccgct ggtcattgcc ggcgacgacc gcatggtgtg
     3601 ccgcgtcgcg tggtgctccg gggcggccca gggattgttc gagcaagcga tcacgctcgg
     3661 tgtcgacctc tacctgagcg gcgagatctc cgaaccgacg gtgcatctgg cacgcgagtc
     3721 cggcgtcgcc tatattgctg ccggtcacca cgccagcgaa cgctacgg
//
LOCUS       DAOSIT010000193         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_21733_length_3722_cov_9.184892, whole genome shotgun sequence.
ACCESSION   DAOSIT010000193 DAOSIT010000000
VERSION     DAOSIT010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21733_length_3722_cov_9.184892"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLL14_05890"
     CDS             complement(<1..146)
                     /locus_tag="PLL14_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOG03489.1"
                     /translation="MLPTIAVTTGDPAGIGPDICLRLLDDASAPRFGARIVVLADRSL
                     LAERA"
     gene            complement(152..1456)
                     /locus_tag="PLL14_05895"
     CDS             complement(152..1456)
                     /locus_tag="PLL14_05895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG03490.1"
                     /translation="MTFLLRAILFFACLVGLATAQSRTPAQPIPVDRIVAVVNDEVIT
                     LYELRSRLDSALGQLQRQGMPLPPRDVLEKQMLERLVIDKVQLQQARDMGLRVDDGQL
                     EQALQRIAAGNNLSLSQFRAALEKDGITFASFREEIRSEMTIARVREREVDSKIFISD
                     GEIDNYLTSASGLGSAGEEYQLAHILLRAPESASPEQIQKLRAKADQVLERLRKGEDF
                     AQLAAAYSDAPDGLKGGNLGWRPLDRLPAMFAEVGVTLKVGEVSPVLRSSNGFHLIKL
                     LAKRGGAAAQAVEQTHARHILIKVNEVVSESEARHKLEGLRERVKHGESFAELAKLFS
                     QDGSASKGGDLGWIYPGDTVPEFERAMNLLAPGELSQPVQSPFGFHLIEVLERRVQDV
                     SSERQRAAARQALRERKRDEAYQDWLRQARDRAYVELRLEER"
     gene            complement(1467..>3722)
                     /locus_tag="PLL14_05900"
     CDS             complement(1467..>3722)
                     /locus_tag="PLL14_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HOG03491.1"
                     /translation="GVVDREVLAEDEAELRKVGTVLTADRLTYWPVDDEVEAVGSVRL
                     QQDDDVISGPKARLKLEDQVGFFDQASYFVKRQSAPTRTPTDDTAAALWDANTPDNAS
                     ATGFAAPRALNIKPGQTKVKTAKKPANELTEGRGEAERIDFEGENQRRLTSATYTTCK
                     PGNDDWYLSVGDLQLDYDNDVASGTDGTVRFMGVPILHSPWVSFSLNNERKSGFLTPS
                     YGSSSDNGFEFVLPYYWNIAPNMDATFDTRILSKRGLQLGTEFRYLNTAYGGTYNGKA
                     AVEYLPHDRLRDIDRWGLGLTHSQNSSNGFSGAINYSRVSDNYYYTDLSSQITSTAKT
                     QLLQQGLLSYSGGGWWDVTTNVQSFQTLQPDPQNPVSKPYRLLPQITLNARQPDLYST
                     DTALFGQYTYFAHPDGSMVEGQRMVLQPQVSLPYVTPGWYVIPRVAANLTRYALSYPN
                     SVSVGDTSISRTLPIFSVDSGMTFERPSNWFGRDYTQTLEPRLFYLNIPYQNQNQIPI
                     FDTSLADFNFAQIFADNQFSGWDRINNANQLTAAVSSRLIDPSNGSEIMRAMVGQRFN
                     FTDEKVALPGSSPPQKGDRSDFLAAFSGQLLPRLYADAALQYDPGDQQFQRYAIGARY
                     MPEAGKVLNATYSYNSEAAIPINQVDLSGQWPINSRWQAVGRYNYSFTNSLPVEIIGG
                     LEYNAGCWAVRTVAHRIQTTQNNSTTQFFVQLELNGFASIGSNPLSILQRRIPGYAVG
                     PALSDPALAER"
BASE COUNT          725 a         1147 c         1108 g          742 t
ORIGIN      
        1 gcccgttccg caagcagcga acggtccgcc aacaccacga tgcgtgcgcc aaagcgcggc
       61 gcgctcgcgt catcgaggag acgcaggcag atatcggggc cgatacccgc aggatccccg
      121 gtcgtgacgg caatcgtcgg caacaagccg ctcaacgttc ctccagccga agctcgacgt
      181 aggcccggtc gcgggcctgg cgcagccagt cctggtaggc ctcgtcgcgt ttgcgttcgc
      241 gcaaggcctg acgcgccgcg gcgcgttgac gctcgctcga tacgtcctgg acacgacgtt
      301 caaggacctc gatcaaatgg aaaccgaacg gcgactggac cggttgactg agttctcctg
      361 gagccaggag gttcatcgct cgttcgaact ccggaacggt gtcgccggga taaatccagc
      421 cgagatcccc gccttttgat gccgatccgt cctgcgagaa aagcttggcg agctcggcaa
      481 agctttcgcc gtgcttgacc cgttcgcgca gaccctccag tttgtgtcgg gcctcggact
      541 ccgacaccac ttcgttgacc ttgatgagga tatgccgcgc gtgcgtttgc tcgacggcct
      601 gtgcggcggc gccgcctcgt ttggccagca gcttgatcaa atggaaaccg ttcgaactgc
      661 gcagaaccgg cgatacctcg ccgaccttca gcgtcacccc tacctcggcg aacatggccg
      721 gcaaccggtc cagcggacgc catccaagat tgccaccctt caggccgtcc ggagcgtccg
      781 aataggccgc cgcgagctgc gcaaaatcct cacctttgcg caaccgctca aggacctggt
      841 cggccttggc acgcagcttc tggatctgtt ccggactcgc cgactcgggc gcacgcaaca
      901 gaatatgcgc aagctggtac tcttccccag cgctacccag gccggatgca ctggtcaggt
      961 agttgtcgat ctcgccgtcg gaaatgaaga tcttgctgtc cacctcacgc tcacgtaccc
     1021 gggcaatcgt catttcggaa cgaatctcct cgcgaaagct tgcaaaggtg atgccgtcct
     1081 tttccagagc tgcccgaaac tgactgagcg acaggttgtt gcccgcggca atgcgttgca
     1141 gtgcctgttc aagttgtccg tcgtcgacgc gcaaacccat gtcgcgtgcc tgctgcaact
     1201 gcaccttgtc gatcaccagg cgttcgagca tctgtttttc cagcacatcg cgcggcggta
     1261 gcggcatccc ctgtcgctgt aattgcccca gcgccgagtc gagacgcgag cgcaattcgt
     1321 aaagggtgat cacctcgtcg ttgaccacgg cgacgatacg gtcaacggga atcggctggg
     1381 ccggcgtccg cgattgcgcc gtcgccagtc cgaccaggca ggcaaagaaa agtatcgcgc
     1441 ggagtagaaa cgtcatgtcc tttaacctac cgttcggcca gcgccgggtc gctcaaagcc
     1501 ggaccgaccg cgtagcccgg gatccggcgt tggagaatac tcagtggatt cgagccaatg
     1561 ctcgcgaagc cattcaattc aagttgcacg aagaactgtg tcgtcgagtt gttctgggtg
     1621 gtctgaatcc ggtgggctac cgtacgtact gcccagcaac ccgcgttgta ctccagcccg
     1681 ccgatgatct cgaccggcag ggaattggta aacgagtagt tgtaacggcc gaccgcctgc
     1741 cagcgggagt tgatcggcca ctgcccggaa aggtcgacct gattgatcgg aatcgccgcc
     1801 tcgctgttgt aactgtaggt cgcattgagc accttgcccg cctcgggcat gtagcgcgcc
     1861 ccgatcgcgt agcgttgaaa ctgctggtca ccggggtcgt actgcagtgc ggcgtccgcg
     1921 tacagccggg gtaacaactg gccgctgaaa gcggccagga aatcggatcg gtcgcctttt
     1981 tgcgggggcg aactacctgg cagcgccacc ttttcgtcag tgaagttgaa ccgctgcccc
     2041 accatcgcgc gcatgatttc gctcccgttg gacgggtcga tcagccggga acttacggct
     2101 gcggtcaact ggttggcatt gttgatgcga tcccagccgg aaaactggtt gtcggcaaag
     2161 atctgcgcga aattgaagtc cgccagactg gtatcgaaga tcgggatctg gttctggttc
     2221 tggtacggga tattcagata gaacaggcga ggctccagag tctgcgtata gtcccgacca
     2281 aaccagttgc tcggccgctc gaaggtcatt cccgaatcga cactgaagat cggcagtgtg
     2341 cggctgatcg aagtatcgcc gacactgacc gagttcgggt acgacagggc gtaccgggta
     2401 agattggcgg ccacccgcgg aatcacgtac cacccgggag tgacgtacgg caaggacacc
     2461 tgcggctgca gcaccatcct ctgcccttca accatgctgc cgtcgggatg ggcaaaatag
     2521 gtgtattgac cgaagagtgc cgtgtccgtg gagtacagat cgggttgacg cgcgttcagg
     2581 gtgatctggg gcaacaaacg atagggcttg ctgaccggat tctggggatc gggttgcagc
     2641 gtctggaagg actgtacgtt ggtggtcacg tcccaccatc cgccgccact gtaagacagc
     2701 aaaccctgct gcaggagttg cgtcttcgcg gtgctggtga tctggctcga caaatcggta
     2761 tagtagtagt tgtccgacac ccggctgtaa ttgatggcgc cggaaaatcc attcgacgaa
     2821 ttctggctgt gcgtcagccc aagtccccag cgatcgatat ccctcaaccg gtcatgcggc
     2881 aaatactcga cggcagcctt gccattgtag gtccccccat aggccgtgtt caggtagcga
     2941 aactcggtcc cgagctgcaa acctcgcttg ctgagaatac gcgtgtcgaa ggtcgcatcc
     3001 atgttcggcg cgatattcca gtagtacggc aggacgaact cgaacccgtt gtcgctgctg
     3061 gaaccgtaac tcggcgtcag gaaacccgac ttgcgctcgt tgttgagcga aaatgacacc
     3121 cacggtgaat ggagaatcgg cacccccatg aagcgaaccg tgccgtcggt accactggca
     3181 acgtcgttat cgtagtccag ttgcaggtcg ccgacactga gataccaatc gtcgttaccg
     3241 ggcttgcacg tcgtgtaggt agcagaagtc agacgccgct gattttcccc ttcgaaatcg
     3301 atgcgctccg cctcaccccg cccctcggtc aactcgtttg ccggtttttt tgcggtcttg
     3361 accttggtct gtcccggctt gatgttcagt gcgcgcgggg ctgcaaaacc ggttgccgac
     3421 gcattatccg gggtgttcgc gtcccacagc gcagccgcgg tgtcgtcggt cggagtacgg
     3481 gtcggagccg actgccgctt aacgaagtac gaagcctggt cgaaaaaacc gacctggtct
     3541 tcgagcttga ggcgcgcctt cgggccggaa atcacgtcgt catcctgctg caggcgaacg
     3601 ctgccgacgg cctcgacctc gtcatccacc ggccagtaag tcaggcgatc agcagtcaac
     3661 accgtgccta ccttgcgtaa ctccgcctca tcctcggcaa gcacctcgcg gtcgacgacg
     3721 cc
//
LOCUS       DAOSIT010000194         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22060_length_3683_cov_9.511852, whole genome shotgun sequence.
ACCESSION   DAOSIT010000194 DAOSIT010000000
VERSION     DAOSIT010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22060_length_3683_cov_9.511852"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLL14_05905"
     CDS             complement(<1..899)
                     /locus_tag="PLL14_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HOG03492.1"
                     /translation="MSAGIVVRGLRKVRTLLADSGGARGLAGKELVRTRELLHECAAG
                     VGGEISARQRAGRLAETYLRLDDEGRSTFLRTLATEFGPDPQLVATAHAGYQAAIGSD
                     AQWMAESELRKAMRSSRLRILAQFNTLPQGVKFLVDLRADLLRFADTDAALRSLDREL
                     ESRLGAWFDVGFLELQRITWSSPAALLEKLIEYEAVHEIRSWSDLKNRLDSDRRCYAF
                     FHPRMPMEPLIFVEVALTDHLADNVQMLLDEQAPVFDAQRASTAIFYSISNTQPGLRG
                     VSFGNFLLKRVVDDLKRDYPRLV"
     gene            complement(945..1742)
                     /gene="lgt"
                     /locus_tag="PLL14_05910"
     CDS             complement(945..1742)
                     /gene="lgt"
                     /locus_tag="PLL14_05910"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOG03493.1"
                     /translation="MLIHPQFNPVAVSIGPLSVHWYGLMYLVAFLQFWWLGRRRILTR
                     PELTTAGWTLRQLDDLLFYGVLGVIAGGRLGQVLFYEPAYYLEHPLQVLAVWRGGMSF
                     HGGFLGVLIAMWLYARKSRRRWLELTDFIAPLVPLGLAAGRIGNFINGELWGRAADPA
                     LPWAMLFPWVDDVPRHPSQIYQAGLEGLVLFICLWLYSSRKRPRGAVSGAFLIGYGVL
                     RWVAEYFRTPDEGIFGLSYTVSMGQWLSLPMVLFGIIFVVAAHRRRV"
     gene            1827..3683
                     /gene="ilvD"
                     /locus_tag="PLL14_05915"
     CDS             1827..3683
                     /gene="ilvD"
                     /locus_tag="PLL14_05915"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOG03494.1"
                     /translation="MPHYRSRTSTHGRNMAGARALWRATGMQDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTVAIDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRVNIPTIFVSGGPMEAGKVKWGGKVI
                     AVDLVDAMVKGGDSNCSDDEVDSFERSACPTCGSCSGMFTANSMNCLTEALGFSLPGN
                     GTVLATHADRRELFLKAGRQIVDLCRRYYEQDDTSVLPRSIGSFAAFENAMTLDVSMG
                     GSTNTVLHILAAAQEGGVSFTMDDIDRISRQVPCLAKVAPATPDYHIEDVHRAGGIFG
                     ILGELDRAGLLHREVPTVHARTLGEAIDNWDVMRTQAPQLCEFFKAAPGGIPTQKAFS
                     QNRRYPELDLDRAKGCIRDKAHAYSQEGGLAVLYGNIARDGCIVKTAGVDESIWVFAG
                     KARVFESQDDAVQAILGDQVLPGEVVVIRYEGPKGGPGMQEMLYPTSYLKSKGLGKVC
                     ALLTDGRFSGGTSGLSIGHVSPEAAEGGAIGLVQEGDLIEIDIPGRRIHLAIAESELA
                     RRRVAMESKAAAAWRPAVARQRVVSQALQAYALMTTSAATGAVRDLKQLQRR"
BASE COUNT          706 a         1132 c         1197 g          648 t
ORIGIN      
        1 ctgaccagac gcgggtagtc gcgtttcagg tcgtcaacga cgcgcttgag caggaaattg
       61 ccaaaggaca cgccgcgcag gccgggttgc gtgttggaaa tcgaatagaa gatcgccgtg
      121 ctggcacgct gcgcgtcgaa aaccggcgcc tgttcgtcga gcagcatctg cacattgtcc
      181 gccaggtggt cggtcagcgc gacctcgacg aagatcagcg gctccatggg catgcgcggg
      241 tggaaaaagg catagcagcg acggtcggaa tcgagacggt tcttcaggtc gctccaggaa
      301 cggatctcat gcaccgcctc gtattcgatc agtttttcga gcagcgctgc cggcgaactc
      361 caggtaattc tttgcagctc aaggaaaccg acgtcgaacc aggcgcccag ccgggactcg
      421 agttcgcggt cgagcgagcg cagcgcagcg tccgtgtcgg caaagcgcag caggtcggcg
      481 cgcaggtcga ccaggaattt gacgccttgc ggcagggtat tgaattgcgc caggatacgc
      541 aggcgcgacg agcgcatggc cttgcgcagt tccgattcag ccatccattg cgcgtcactg
      601 ccaatggccg cctgatagcc ggcatgtgcc gtggcgacca actgcgggtc ggggccgaat
      661 tcggtggcca gcgtgcgcag gaaggtcgag cgaccctcgt catcgagacg caggtaggtt
      721 tccgccaaac gcccggcacg ctgcctggcc gagatttctc cgccgactcc agctgcgcat
      781 tcgtgaagga gttcgcgcgt ccggaccagt tccttgcccg caaggccacg tgcgccgcca
      841 ctgtcggcaa gcagcgtgcg caccttgcgc aagccccgga ctacaattcc tgcagacatc
      901 tctttctcct cggtctcgcg gccggccacg acacgtcgtt ctgctcagac gcgtcggcga
      961 tgcgccgcga cgacaaaaat gataccgaac aggaccatcg gcagcgacag ccactgtccc
     1021 atgctgaccg tgtacgaaag gccaaagata ccctcgtccg gcgttctgaa atactcggca
     1081 acccagcgca ggacgccgta accgatcaga aaggctcccg aaacggcacc acgaggtcgc
     1141 ttgcggctcg aatagagcca caggcaaata aacagaacaa ggccctcaag accggcctgg
     1201 tagatttgcg aagggtggcg cggtacatcg tctacccagg ggaaaagcat cgcccacggc
     1261 agcgccggat cagccgcgcg cccccagagt tcgccattga tgaagttgcc gatgcgcccg
     1321 gcagcgaggc ccagtggcac cagcggggca atgaaatcgg tgagttcgag ccagcgccgc
     1381 ctgctcttgc gggcgtatag ccacatggcg atcagtacgc caaggaagcc gccgtgaaac
     1441 gacatgccgc ctcgccatac ggcgaggact tgcagcgggt gctccaggta gtatgccggc
     1501 tcgtagaaca ggacctggcc aagacggccg ccggcgatga ctcccaggac gccgtagaac
     1561 agcaggtcat cgagctgtcg cagcgtccag ccggcagtgg tcaactcggg acgggtcagg
     1621 atccgtcgcc ggcccagcca ccagaactgc agaaaggcca ccaggtacat caatccgtac
     1681 cagtgcacgc tgagcgggcc aatcgagacg gcgacagggt tgaactgcgg atggatcaac
     1741 acgttgcgaa caccccgggg gcctttcgga tagaattcgc attatatcgc gccaccctcg
     1801 ggcgtgttct ggattggaga tcggcaatgc cccactatcg ttcccgcacc tcgacacatg
     1861 gccgcaacat ggcgggcgcg cgcgccctgt ggcgcgccac cggcatgcag gacggagatt
     1921 tcggcaagcc gatcattgcc gtcgtcaatt cgtttaccca gtttgttccg gggcacgtgc
     1981 atctcaagga tctagggcag ttggtcgctc gcgaaatcga ggccgccggc ggtgtcgcca
     2041 aggaattcaa taccgtcgcc attgatgacg gcattgccat ggggcacgac ggcatgcttt
     2101 attcattgcc ttcgcgtgac ctgatcgccg actccgtcga atacatggtc aacgcacact
     2161 gcgccgacgc gatggtgtgc atttcgaact gcgacaagat cactccgggg atgttgatgg
     2221 ccgcgatgcg cgtgaacatc ccgaccattt ttgtctccgg cggcccgatg gaggccggca
     2281 aggtgaagtg gggcggcaag gtgattgccg tcgacctggt cgacgccatg gtcaagggcg
     2341 gcgacagcaa ttgttccgac gatgaggtcg actccttcga gcgctcggcg tgtcccacct
     2401 gcggttcgtg ttcagggatg tttaccgcca attcgatgaa ttgcctgacc gaagcgctgg
     2461 ggttttcgct gcccggcaac ggcaccgtcc tggccacgca tgccgatcgc cgcgaacttt
     2521 tcctcaaggc cgggcgccag attgtcgacc tctgtcgtcg ttattacgag caggacgaca
     2581 cctcggtgct gccgcgctca atcggcagtt tcgctgcctt cgagaacgcg atgacgctgg
     2641 atgtgtcgat gggggggtca acgaatacgg ttctgcacat tctggcggcg gcacaggagg
     2701 gtggcgtcag cttcacgatg gacgacatcg accgcatctc gcgccaggtg ccctgtctcg
     2761 ccaaggtggc gcccgccaca cccgactacc atatcgagga cgtgcaccgc gcgggtggca
     2821 tcttcggcat tctcggcgaa ctggatcgcg ccggtctgct ccatcgcgag gtgccgacag
     2881 tgcatgcgag gacgctcggt gaggcgatcg acaactggga tgtgatgcgt acgcaggcgc
     2941 cccaactctg cgagttcttc aaggcggcgc cgggtggcat tccgacccag aaggccttct
     3001 cgcagaaccg gcgttacccg gagctcgacc ttgatcgcgc caagggttgc attcgcgaca
     3061 aggcgcatgc gtactcgcag gaaggtggcc tggccgtgct ttacgggaac atcgccagag
     3121 acggctgcat tgtgaaaact gccggtgtgg acgagagcat ctgggtcttt gccggcaagg
     3181 cacgggtgtt cgagagccag gacgatgccg tccaggcgat tctcggcgac caggtgctgc
     3241 ccggcgaggt ggtggtcatt cgctacgaag gcccgaaagg tgggccgggc atgcaggaaa
     3301 tgctctatcc cacgtcctac ctgaagtcga aggggctggg caaggtctgc gcactgctca
     3361 ccgacggccg cttttccggt ggtacctctg gactgtcgat cgggcatgtt tcccccgagg
     3421 ccgccgaagg tggcgcgatc ggcctggtgc aagagggcga cctgatcgag atcgatattc
     3481 cggggcgtcg tatccacctg gcgatcgccg agtccgagct ggcccggcgg cgggtggcga
     3541 tggagtcgaa agccgcggcg gcctggcggc cggcggttgc gcgccagcgt gtcgtgtcgc
     3601 aggcgctgca ggcctatgcg ctgatgacca cctcggcggc taccggcgcg gtgcgcgatc
     3661 tgaagcagtt gcagcggcgc tga
//
LOCUS       DAOSIT010000195         3683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22062_length_3683_cov_8.420893, whole genome shotgun sequence.
ACCESSION   DAOSIT010000195 DAOSIT010000000
VERSION     DAOSIT010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22062_length_3683_cov_8.420893"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLL14_05920"
     CDS             <1..186
                     /locus_tag="PLL14_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOG03495.1"
                     /translation="PDIKEVLFAKRILEAMPDGSGVATIDGKMQDDATWKQARVIVDL
                     ARLVARRDPELAAAYGL"
     gene            220..1278
                     /locus_tag="PLL14_05925"
     CDS             220..1278
                     /locus_tag="PLL14_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOG03496.1"
                     /translation="MSEKTRLGNFFEDFWVGQKIAHATPRTITEGDVALYTALTGSRF
                     ATNSADTFAHSLGFPRAPVDDLLAFNMVFGRTVPDISLNAIANLGYAAGRFGHPIYPG
                     DTLTADSTVIGLKENSDGKTGIVYVRSCGVNQRGQIALDYCRWVMVRKRNPQSPAPAA
                     SVPDLPSAVAAADLIVPTGIRIEQYDCALAGSSELWDDYRVGERIDHVDGMTVEESEH
                     MMATRLYQNTARVHFNQQAEQAGRFGRRIIYGGYIISLARALSFNGLANAFSIAAING
                     GRHVSPAFAGDTVYAWSEVIAQLIIPGRHDLGALRLRTVATKDHPCADFPYKSPDGRY
                     DASVLLDFDYTVLIPRRA"
     gene            1563..>2077
                     /locus_tag="PLL14_05930"
     CDS             1563..>2077
                     /locus_tag="PLL14_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG03497.1"
                     /translation="MFQVRIHGRGGQGVVTAAEMLSIAAFEEGRHAQAFPSFGSERTG
                     APVVAFCRIADKEIRLREPIMEPDALIIQDPTLLYQVDVFSGLKKGGYILINTTRSFD
                     ELGLGDFVRDWPKERLCTVPATDLSRQHVGRPMPNVPLLAGFAAASGVIQLESVINAI
                     RTKFPEKIANS"
     assembly_gap    2078..2177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2178..3014
                     /gene="porA"
                     /locus_tag="PLL14_05935"
     CDS             <2178..3014
                     /gene="porA"
                     /locus_tag="PLL14_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOG03498.1"
                     /translation="QEALDLHIQAFKLAEEVSLPVMVCMDGFILTHAYERVDVPTQEQ
                     VDAFLPPYEPRQVLDPTQPVSIGAMVGPEAFSEVRYLAHAKQMQALERIPQLAEQFKE
                     VFGRDSGGLTHSYLTDDAETIVVAMGSVLGTIKDTVDEMRAAGDKIGVLGITSYRPFP
                     IDSVRAALQNAQRVVVLEKSLAVGIGGILSTDVRMAMSGLQLHGHTVVAGLGGRAITR
                     KSLRALFAQAARGELGHLTFLDLDWNVVNKQLERERTTRRSGPAAESMLRDVGVIAAR
                     IG"
     gene            3026..>3683
                     /locus_tag="PLL14_05940"
     CDS             3026..>3683
                     /locus_tag="PLL14_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG03499.1"
                     /translation="MSFQPLHFYQTGTFTIGNRLLAEEDLSVQSDIERTNSLNSGHRA
                     CQGCGEALGARFAIDAAMKAARGRLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAAIKVKRQKGEMGDVRVVAQGGDGGTTDIGFGCLSGMFERNDDVLYICYD
                     NGGYMNTGVQRSSATPPAARTATTMPVGPEPGNAFGQGKFVPAIAMAHEIPYVATATV
                     A"
BASE COUNT          681 a         1165 c         1089 g          648 t
ORIGIN      
        1 cccgacatca aggaagtgct gtttgccaag cgtatcctgg aagcaatgcc cgatggcagc
       61 ggcgtggcga cgatcgacgg caagatgcag gacgatgcga cctggaagca ggcccgcgtc
      121 atcgtcgatc tggcccgcct ggttgccaga cgcgacccgg agctggccgc agcctatggc
      181 ctctagaccc tttactcaag accacacgga gaaacgccag tgagcgaaaa gaccaggctg
      241 ggcaacttct ttgaagactt ctgggtcggg cagaaaatcg cccacgcaac accgagaacg
      301 attaccgaag gggacgtggc gctatacacc gcgctcaccg gttcgcgctt tgccaccaat
      361 tcggccgaca ccttcgcgca cagcctgggt ttcccgcggg cgccggtgga cgatctgctg
      421 gcgttcaaca tggtctttgg ccgcacggtg ccggatattt cactcaacgc catcgccaac
      481 ctcggttacg cggccggccg ctttggtcat ccgatctatc caggggatac actcaccgcc
      541 gattcgacgg tgatcggcct caaggaaaac agcgatggca agaccggcat cgtctatgtg
      601 cgctcgtgtg gcgtcaacca gcgtgggcag atcgcactcg actattgccg ctgggtgatg
      661 gttcgcaaac gcaatcccca atccccggca cctgccgcca gtgtgcccga tctgcccagc
      721 gccgtcgctg ccgccgactt gattgtgcca accgggatca gaatcgaaca gtacgactgc
      781 gcgctggccg gcagttccga gttgtgggac gactaccggg tcggcgagcg catcgatcat
      841 gtggacggca tgaccgtcga ggaaagtgaa cacatgatgg ccacccgtct gtaccagaac
      901 accgcgcgcg tgcatttcaa ccagcaggcc gagcaggcgg ggcgcttcgg gcgtcgcatc
      961 atctatggcg gctacatcat cagtctggcg cgcgcgctgt ccttcaatgg cctggccaac
     1021 gccttcagca ttgcggcgat caatggtggc cgacatgtct ccccggcatt cgccggggat
     1081 acggtatacg cctggtcgga agtgatcgcg cagttgatca ttcctgggcg tcacgatctc
     1141 ggagccctgc ggctacgcac cgtagccacc aaggaccacc cctgtgccga cttcccctac
     1201 aagagccccg acggccgtta cgacgcgagc gtgttgctcg atttcgacta tacggtgctg
     1261 atcccgcgcc gggcgtagcg gcgtccttcc gagtcggcgg gagcagcggc gacaccgctg
     1321 gcacgcgccg aagtcgcgcc ttgccggtga atgccgctgc tgctgcgcac cggcaagacg
     1381 cccctgtttc tgcccgttta ccgaaccgca actgttggcg gatgcacctc catgcgtccc
     1441 cggagatgac ctgctccgtg cctcgcgctg cagccgtgag ttggttgctt gttttcaatt
     1501 cgttcgaagt aagatatcct gagcgggcgt caagagaaaa ttgaaaacaa cgaggaggaa
     1561 acatgttcca agtccgaata cacggtcgcg gcggccaagg cgtggttacg gctgccgaga
     1621 tgttgtcgat tgctgcgttt gaggaaggcc gccacgctca ggccttcccg agtttcggtt
     1681 ccgagcgtac tggagcccca gtcgtcgcgt tctgccgtat cgccgacaag gaaatccggc
     1741 tgcgcgaacc gatcatggaa cccgatgcac tgatcatcca ggatccaacg ctgctgtacc
     1801 aggtcgatgt cttctctggc ctgaagaaag gcggttacat cctgatcaac acgacgcgca
     1861 gttttgacga gttggggctg ggcgatttcg tccgtgactg gccaaaggaa aggttgtgca
     1921 cggtccccgc caccgacctc agtcgccagc acgtcggtcg tcctatgccg aatgtgccct
     1981 tgctggccgg ctttgccgcc gcgtccggcg tcattcagct ggagtcggtg atcaacgcca
     2041 tccggaccaa gttcccggag aagatcgcca acagcaannn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnncag gaagccctcg atctgcacat ccaggccttc aagctggctg
     2221 aagaagtgag cctgccggtc atggtgtgca tggacggctt cattctgacg cacgcgtacg
     2281 aacgtgtgga cgtccccacc caggagcagg tcgacgcctt cctgcccccg tacgaacctc
     2341 gccaggtgct ggacccgaca cagccggtgt cgatcggcgc gatggtcggt ccggaggcct
     2401 tctcggaagt tcgctacctc gcgcacgcca agcagatgca ggcgctcgaa cgtatcccgc
     2461 aactggctga gcaattcaag gaagtgtttg gtcgcgactc cggcggcctg acacattcgt
     2521 acctgacaga cgatgccgaa accatcgtcg tggccatggg ctcggtgctg ggcaccatca
     2581 aggacaccgt cgacgagatg cgtgctgccg gcgacaagat cggtgtgctg ggcatcacct
     2641 cttaccggcc tttcccgatc gacagcgtcc gcgccgccct gcagaacgcg cagcgcgtgg
     2701 tggtcctcga aaagagcctt gccgtcggca tcgggggcat cctgtccacc gacgtgcgca
     2761 tggccatgtc cggtctgcaa ctgcatgggc ataccgtagt ggccgggctc ggggggcgag
     2821 cgattaccag aaaatcgctg cgcgcgctgt ttgcccaggc cgcgcgcggt gaactgggtc
     2881 acctcacctt cctcgatctc gactggaacg tggtcaacaa gcagctcgaa cgcgagcgca
     2941 ccacgcgccg ctcgggcccg gcagccgaaa gcatgctgcg cgacgtgggc gtcattgccg
     3001 cccgtattgg ctgaggacat cgacgatgag cttccaaccc ctgcattttt accagaccgg
     3061 caccttcacc atcggcaacc gcctgctcgc cgaagaagac ctcagcgtgc agtccgacat
     3121 cgagcgtacc aattcactca actccggaca ccgcgcctgc cagggctgtg gcgaagcact
     3181 tggcgcgcgt ttcgccatcg acgcggcgat gaaggccgcg cgtggccgcc tgatcgcggc
     3241 caatgccacc ggttgcctgg aggtcttctc gacaccctac cccgagacct cgtggcagat
     3301 cccctggatc cactcgctgt tcggcaatac cgctgcagtc gccaccggca tcgccgcggc
     3361 gatcaaggtg aaaagacaaa agggcgagat gggtgacgtc cgcgtcgtgg cccagggcgg
     3421 cgacggtggt accaccgaca tcggcttcgg ctgcctgtcg ggaatgttcg aacgcaacga
     3481 cgacgtgctg tacatctgct acgacaacgg tggctacatg aacaccggcg tgcaacggag
     3541 ttcggccacc ccaccggcgg cccgcacggc aaccaccatg ccggtcggcc cggagcctgg
     3601 caacgctttt ggtcagggca agttcgttcc ggcgatcgcc atggcgcacg agattcccta
     3661 cgtcgcgaca gccaccgtcg ccg
//
LOCUS       DAOSIT010000196         3674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22134_length_3674_cov_7.668693, whole genome shotgun sequence.
ACCESSION   DAOSIT010000196 DAOSIT010000000
VERSION     DAOSIT010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22134_length_3674_cov_7.668693"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLL14_05945"
     CDS             <1..180
                     /locus_tag="PLL14_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOG03500.1"
                     /translation="RVSMDMLACDLTDLPGTGVDSPVVLWGDGVPADEVATSAGTISY
                     ELFCALARRVPVRTV"
     gene            197..1570
                     /gene="radA"
                     /locus_tag="PLL14_05950"
     CDS             197..1570
                     /gene="radA"
                     /locus_tag="PLL14_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOG03501.1"
                     /translation="MSRASLKTVFTCSDCGASTGKWQGQCPGCGVWNTLVETLPEAPL
                     VGGQKRFEPIGGAAVLQTLSDIEARDEDRLPTGIGEFDRALGGGLVNGGVVLIGGDPG
                     IGKSTLLLQALAALSTAHAVLYVSGEESGQQIAMRARRLSLDTGQLRLLAEINLERIL
                     ATLRSAQPQVAVIDSIQTLWSEQLSSAPGSVAQVRECAAQLTRLAKQTGITVILVGHV
                     TKEGALAGPRVLEHIVDTVLYFEGDTHSSFRLIRAVKNRYGAVNEIGVFAMTDRGLKG
                     VSNPSAIFLSQHGTDVPGSCVLVTQEGTRPLLVEIQALVDQAHGNPRRLTVGLDAQRL
                     AMLLAVLHRHAGIACFDQDVFVNAVGGVKIAEPAADLAVLLAIVSSLKNRSLPAKLIV
                     FGEVGLAGEIRPAPRGQERLREAAKLGFTRAMIPEANKPRQAIAGIEVFAVRRVEEAV
                     QRLRELD"
     gene            1812..3149
                     /locus_tag="PLL14_05955"
     CDS             1812..3149
                     /locus_tag="PLL14_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG03502.1"
                     /translation="MAVAPETAIMALYCDFENVALGVRDAKYDKFDIKPVLERLLLKG
                     SIVVKKAYCDWERYKGFKATMHEASFELIEIPHLRQSGKNSADIRLVVDALDLCYTKS
                     HVDTFVIISGDSDFSPLVSKLRENAKYVIGVGVKQSTSDLLIANCDEFIFYDDLVREM
                     QRSARRDSKEAQPVVRRSPEEDAQRRAELEERRSKAIELAVATFDALVAERGDSSKIW
                     ASMLKEAIKRRKPDFNESYYGFRAFGNLLEEAQARALLEIGRDEKSGTYVFRSNGIST
                     RVEATAEAAAEKLLLAPVESVVNADAAVAEESRPQAGAPRKGEGRRKATEKGVKRVRE
                     QRTVKQQQEVSAVEPGAPVTESVAADEVIPCVTVEVVPLAPEAETLQGSPELPPVADA
                     RVAETPADRIDAATDAAEQVTVDDGSEEPKPVKKPASRSRRPRKPKVLPEETP"
     gene            complement(3240..3623)
                     /locus_tag="PLL14_05960"
     CDS             complement(3240..3623)
                     /locus_tag="PLL14_05960"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOG03503.1"
                     /translation="MQRTMLKSKLHRVSATHAELHYEGSCAIDEDLLDAANIREYEQI
                     DIWNVNNGARFTTYALRAERGSGVISVNGSAARRAAPGDILIIASFAAYTEVELSKYA
                     PQLIYVDTQNRIVRIAGQVPTQAAA"
BASE COUNT          705 a         1064 c         1216 g          689 t
ORIGIN      
        1 cgggtgtcga tggacatgct ggcctgcgac ctgaccgatc tgccgggtac cggggtcgat
       61 agtccggtgg tcctctgggg agacggcgtg ccagccgacg aagtcgccac ctctgccggt
      121 accatcagct acgaattgtt ctgtgccctg gcgcgccgcg taccggtgcg cacggtctga
      181 gcatgggggt cagggcgtgt ccagggccag tctgaagacc gtgtttacct gcagcgattg
      241 cggtgccagc accggcaagt ggcaagggca gtgtcccggc tgcggggtct ggaatacgct
      301 ggtcgaaacg cttcccgaag cgcctctcgt cggtggccag aaacgcttcg agccgatcgg
      361 cggcgcggcg gtactgcaga ccctttccga catcgaggcg cgcgacgaag accgcctgcc
      421 gaccgggatc ggagaattcg accgggcact gggtggcggg ttggtcaacg gtggcgtggt
      481 gctgatcggt ggcgatccgg ggatcggcaa gagcacgctg ttgctgcagg cacttgcggc
      541 cttatccacg gcgcacgcgg tgctttacgt cagcggcgaa gagtccggcc agcagatcgc
      601 gatgcgcgcc cgacgcctgt cgctcgatac cggccagctc agactactcg ccgaaatcaa
      661 tctcgaacgt atcctggcca ccctgcggtc ggcgcagccg caggtggcgg tgatcgattc
      721 gatccagacg ctgtggtccg agcaactgag ctcggcaccg gggtcggtcg cgcaggtccg
      781 tgaatgcgcc gcgcaactca cgcgcctggc gaaacagacc ggcatcacgg taatcctggt
      841 cggccatgtg accaaggagg gtgccctggc cggcccacgc gtactcgagc acatcgttga
      901 cacggtgctt tatttcgagg gtgacacgca ctcgagtttt cgcctgatcc gagcggtcaa
      961 gaaccgctac ggcgcggtca acgagatcgg cgtttttgcg atgaccgacc gaggtctgaa
     1021 aggggtgagc aatccctcgg cgattttcct gtcgcagcat ggtaccgatg ttcccggctc
     1081 ctgcgtgctg gttacccagg aaggtacccg gccactgctg gtcgagatcc aggcgctggt
     1141 tgatcaggcg catggcaacc cgcgtcgtct gacggtgggg ctcgatgcgc agcgtctggc
     1201 gatgctgctg gcggtactgc accgccacgc cgggatcgcc tgcttcgatc aggacgtttt
     1261 cgtcaacgct gttggcggcg tcaagatcgc tgaaccggca gccgatctcg cggtgttgct
     1321 ggcgattgta tcgtcgctga agaaccggtc gttacccgcg aaactcatcg tctttggtga
     1381 agtcggcctg gccggagaaa tccgcccggc gccacgcggg caggaacgcc tgcgggaagc
     1441 cgccaagctc ggtttcacac gcgcgatgat tcccgaagcc aacaaaccga ggcaggccat
     1501 tgccggcatc gaagtctttg ccgtgcgacg cgtcgaggaa gccgtacagc ggctgcgaga
     1561 gctggactag aatcgctcgc acgaggcgca gcgagtcgat ctccggctcc agaggccccc
     1621 gtccttgtat tgccccccgt acatcaccgg acaggtagca tcatccgaca cgggatcgca
     1681 cgcacgcgcc gggacttttc cttgcttcct gcaggtattt gtttaccatc ctggcgattg
     1741 agtccaaatt ctgaagcgct tgggcttttc gcctgaagcg tggcacacca attctgtgga
     1801 gagaaggaaa tatggccgta gcgcccgaaa ccgccatcat ggcgctgtac tgtgacttcg
     1861 aaaacgttgc gctcggtgtg cgcgatgcca agtacgacaa gttcgacatc aaaccggtgc
     1921 ttgagcggtt gctgctcaag ggcagtatcg tggtcaagaa agcctattgc gactgggaac
     1981 ggtacaaggg tttcaaggcc accatgcacg aggcgagctt cgagttgatc gagataccgc
     2041 atctgcgaca atcgggcaag aactcggccg acatccggct ggtcgtcgat gccctggatc
     2101 tgtgctacac caagtcacat gtcgatacct tcgtgatcat cagtggcgac tcggatttct
     2161 cacctctggt ttccaagctg cgtgagaacg ccaagtacgt gatcggcgtg ggtgtcaagc
     2221 agtcgacttc agacctgctg atcgccaatt gcgacgagtt catcttttat gacgacctcg
     2281 tgcgcgaaat gcagcgatcg gcgcggcggg attcgaagga agcgcagcca gtagtcaggc
     2341 gctcgccgga agaggatgcg caacgccggg ccgagttgga ggagcgccga agcaaggcta
     2401 tcgagttggc cgtcgccact tttgatgcgt tggtcgcgga acgcggtgac agcagcaaga
     2461 tttgggcgtc gatgctcaag gaggcgatca agcggcgcaa gcccgatttc aacgaatcat
     2521 attacggctt ccgggccttt gggaacctcc ttgaagaggc gcaggcgcgt gcgctgctcg
     2581 aaattggccg agatgagaaa tccgggacct atgtctttcg cagcaacgga atttcaacac
     2641 gcgttgaagc aactgccgaa gcggcggctg aaaagctgct gctggcacca gttgagtcgg
     2701 tcgtgaatgc agatgcagca gttgccgagg aatcccgccc tcaagctggc gcgccgcgaa
     2761 agggggaggg ccggcgcaag gcgacagaga agggcgtcaa gcgggtcagg gagcagcgga
     2821 ccgtcaagca gcagcaggaa gtgtcggcgg tcgagcccgg tgcaccggtc acggaaagcg
     2881 tggcggccga cgaggtgatt ccctgcgtga ccgtcgaggt ggtgccgctc gctccggaag
     2941 cggaaaccct gcagggcagc ccggagctgc cgcccgttgc tgacgcacgc gtggcggaga
     3001 ctcctgccga ccggattgat gctgccaccg acgcggccga gcaggtgacg gtggacgacg
     3061 gttcagaaga acccaagcca gtcaagaaac cggccagccg cagccgtcgc ccgcgcaaac
     3121 cgaaggtgct gccagaagaa acgccctgat ctgcaacctg tcccgtgatg ggaatgggtc
     3181 agcagaaaaa agccgcgacc agggtcgcgg ctttttcggc agggtagcgg cgtgtgccgt
     3241 caggctgctg cctgagtcgg aacttggccg gcaatgcgca caatgcggtt ttgcgtgtca
     3301 acgtagatca gttgcggtgc gtatttggag agttcgactt cggtatacgc cgcgaagctg
     3361 gcgatgatca ggatatcccc cggggccgcg cgacgcgctg ccgaaccatt caccgagatg
     3421 acaccggatc cgcgctcggc gcgcagagcg taggtcgtga agcgtgcgcc gttgttgaca
     3481 ttccagatgt cgatctgttc gtactcacga atgttcgccg catcgagaag gtcttcgtcg
     3541 atcgcgcatg acccttcgta gtgaagttcg gcgtgcgtgg cgctgacgcg gtgcagcttc
     3601 gacttcagca tggttctctg catggatttt caccggttta gagtttgggg aagcgaattg
     3661 tagcggctaa tggc
//
LOCUS       DAOSIT010000197         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22238_length_3659_cov_7.925361, whole genome shotgun sequence.
ACCESSION   DAOSIT010000197 DAOSIT010000000
VERSION     DAOSIT010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22238_length_3659_cov_7.925361"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLL14_05965"
     CDS             <1..302
                     /locus_tag="PLL14_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG03504.1"
                     /translation="EQLAAQQRLVVALTEYARLARLQYDGGYTGYSTVLQAEQTLFPA
                     ELNLASLRASLFASAVNIYKAMGGGWVNEADKLSEADKLSGGGPPATAQMLPPLF"
     gene            complement(343..771)
                     /locus_tag="PLL14_05970"
     CDS             complement(343..771)
                     /locus_tag="PLL14_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03505.1"
                     /translation="MAGSVLLALAGVADAQALFLARRAIGRIEQMSQSSPTTGAAYDT
                     AVVIVEVAPDRVFATIKRLLAQNTEVRVTSSDDARRSVEFTDGTQIGGIQVNPLNDQL
                     SQLLVSTAHPGITTSTTSRIVARIMDVCRELNVVCHPGGS"
     gene            complement(874..3519)
                     /locus_tag="PLL14_05975"
     CDS             complement(874..3519)
                     /locus_tag="PLL14_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03506.1"
                     /translation="MIQQDTFQRLRQGLRAALAVGLVSLALPAAAQQPGSAQVNAIRQ
                     ACRADYQANCAGVPTGGAAAMSCLQENAMYTSPACQQALRAASSGSAATTTTARPAAG
                     ANRVTAADNWPHTLSGASGSAVVYQPQVIAWPERQTLRTRIALSVTPAGATAAVLGTV
                     EVSFATSTDLATRWVTLTNPQLLSSRFPAADTAQAERIDAHLRSALAALPAKRVPLDT
                     ILLSLHQETETPPVVAVHNEPPRIFVSERPASLLIFDGAPVLTPIAGTPLSYAVNTNW
                     NIFLDGDTHTWYWLNNGAWLRASSVEGHWTPAGTLPPAFSGLPNDRNFADVRQQIPGR
                     QLSIADVPQVFVSTVPAEIIVTLGAPQFTAIPGTSLQYVANTDAALFRDAGNGQFYYL
                     VSGRWFSASSLNGPWTFATTRLPPDFARIPASGPRGFVLASVPGTAQAQEALIQARIP
                     QQATLSSSTARLDVVYAGPPKFEPIPGTTMAYAVNTSFNVVQAENSYYACHQGAWFVA
                     ASPLGAWTLAGSVPDAIYTIPPSSPLYPCTYVRVYASTPTTVTYGYTAGYTLGYISAG
                     VMVYGTGFYYPPVIYPGPVPIYYPYPVSYAGATYYNSATGAWARGGAIYGSYYGAAGG
                     TAYNPATGAWARGGAVYGPYGGAGAFSAYNPTTGSYAHGSAVWGANGAAGNASWYNAR
                     TGVSGTTNQNSNAYGRWGSSTVSGPNQTVHTQSQSNARGAAGSFSSSSGAEGAAVRGA
                     GGNSAGAVKTASGNVYAGADGNVYKKTSDGWQKYDNGAWNSPQKPASSFSGTSDRGGA
                     AAGEGARGFDPSAQLEQDHQARFDGAQRQQQFGAMGGQDSFASRFEGGMRGGEGGFGG
                     MRSGGFDGGGGFRGGGGFGGGRFRR"
BASE COUNT          558 a         1250 c         1265 g          586 t
ORIGIN      
        1 aggagcaact cgccgcgcaa cagcggctgg tcgtcgccct gaccgagtac gcgcgtctgg
       61 cacgcctgca gtacgacggc ggctacaccg gctattcgac cgtgctgcag gccgagcaga
      121 cgctgttccc ggccgaactc aatctggcgt ccctgcgtgc ctcgctgttc gcttctgcgg
      181 tgaacatcta caaggcgatg ggcggcggct gggtaaacga agccgacaaa ctcagcgaag
      241 ccgacaaact cagcggcggc ggcccgccgg ccaccgcgca gatgctgccg ccgctgttct
      301 gatgctcgcc agcaaacccg gtggcgcgct tgatcgcgcg cttcaggacc caccgggatg
      361 gcagacgacg ttcaactccc gacacacgtc catgatgcgc gccacgatcc tcgaggtggt
      421 cgaggtggtg atgccgggat gcgcggtcga gaccagcagc tgcgacagct ggtcgttgag
      481 cgggttgacc tggatgccgc cgatctgcgt gccgtcggtg aattccaccg agcgccgggc
      541 gtcatcgctg ctggtcaccc ggacttccgt gttctgggcc agcaggcgct tgatggtagc
      601 gaagacccgg tccggggcga cctcgacgat caccaccgcc gtgtcgtagg cggcgccggt
      661 ggtcggcgac gactgcgaca tctgttcgat gcgcccgatc gcgcgccggg cgagaaacag
      721 cgcctgcgcg tcggcaacgc cggccagcgc cagcaggacg ctgccggcca acaggctgtg
      781 caagtgcagc gatcgcttca tcaacgttct cctctcgaat gtgtcgccag agcgggccgc
      841 gccagcgtcg gtgcagtgcc ccccctggca cgctcagcgc cggaacctgc cgccgccgaa
      901 gccgccaccg ccacggaagc cgccaccacc gtcgaaaccg cccgagcgca tgcctccaaa
      961 cccaccctca ccaccgcgca tgccaccctc gaaacgcgag gcgaaactgt cctggccacc
     1021 catcgcgccg aattgctgct gccgctgcgc gccgtcgaag cgcgcctggt gatcctgctc
     1081 cagttgcgcc gaggggtcga agccacgcgc gccttcaccg gccgctgcac cgccgcggtc
     1141 gctcgtgccc gaaaaactgc tcgccggctt ctgtggcgaa ttccacgcgc cgttgtcgta
     1201 cttctgccag ccgtcggagg tcttcttgta cacgttgcca tcggcgccgg catagacgtt
     1261 gccgctcgcg gtcttgaccg cgccggcgct gttgccgccg gccccacgca ccgcggcgcc
     1321 ctcggcaccg ctgctcgaag aaaacgatcc ggccgcgccg cgcgcattgc tctggctctg
     1381 cgtgtgcacc gtctggttcg gtcccgagac ggtgctcgat ccccagcggc cataggcgtt
     1441 gctgttctgg ttggtggtcc cggaaacgcc ggtgcgggcg ttgtaccagc tggcgttgcc
     1501 ggccgcgcca ttggcgcccc agaccgcgct gccgtgcgca tagctgccgg tggtcggatt
     1561 gtaggccgag aacgcgccgg cgccgccgta cggtccatac accgctccgc cgcgcgccca
     1621 cgcgccggtc gccgggttat aggcggtacc gccggcggcg ccgtagtagg acccgtagat
     1681 cgcgccgccg cgtgcccacg cgccggtggc cgagttgtag taggtggcgc cggcgtagct
     1741 gaccgggtac gggtagtaga tcggcaccgg acccggatag atcaccggcg gataatagaa
     1801 gccggtgccg tagaccatca ccccggcgct gatgtagccc agggtgtagc cggcggtata
     1861 gccgtaggtc acggtggtcg gcgtcgacgc gtagacgcgc acgtaggtgc aggggtagag
     1921 cgggctgctc ggcggaatcg tgtagatcgc gtcgggcacc gacccggcca gcgtccacgc
     1981 ccccagcggc gaggcggcga caaaccacgc cccctgatgg caggcgtaat agctgttctc
     2041 ggcctgcacc acattgaacg aggtgttcac cgcataggcc atcgtcgtgc ccgggatcgg
     2101 ctcgaacttc ggtggccccg catagaccac gtcgagcctg gcggtgctgc tgctcagggt
     2161 tgcctgctgc gggatgcgcg cctggatcag cgcctcctgc gcctgcgccg ttcccggcac
     2221 cgaggcgagc acgaagccgc gcgggccgct ggccgggatg cgtgcgaaat cgggcggcag
     2281 gcgggtggtg gcaaaggtcc acggaccgtt caggctgctt gccgaaaacc agcgacccga
     2341 caccaggtag tagaactggc cgttgccggc gtcgcggaac agcgccgcgt cggtgttggc
     2401 cacatactgc aggctggttc cgggaatggc cgtgaactgc ggcgcgccga gcgtgacgat
     2461 gatctcggcc ggcaccgtgg acacgaagac ctgcggcacg tcggcgatgc tcagctgtcg
     2521 tccgggaatc tgctggcgaa cgtcggcgaa gttgcgatcg ttcggcaatc cggagaaggc
     2581 aggcggcagc gtcccggccg gcgtccagtg tccttcgacg ctgctcgcgc gcagccaggc
     2641 gccgttgttc agccagtacc aggtgtgcgt gtcgccgtcg aggaagatgt tccagttggt
     2701 gttgaccgca tagctgagcg gtgtgccggc gatcggggtc aacaccggtg cgccatcgaa
     2761 gatcagcagg ctggccgggc gctcgctgac gaagatgcgc ggcggctcat tgtgcaccgc
     2821 caccaccggt ggcgtctcgg tttcctggtg aaggctcagg agaatggtgt ccagcggaac
     2881 gcgcttggcg gggagcgcgg ccagcgccga ccggaggtgc gcgtcgatac gttccgcctg
     2941 ggcagtgtcg gcggccggaa aacgcgaact gaggagctgc gggttcgtca gcgtcaccca
     3001 gcgtgtcgcg agatcggtgc tggtggcgaa gctcacctcg acggtgccca gtaccgccgc
     3061 cgtcgcgccc gccggcgtca cactcagtgc gatgcgcgtc cgcagcgtct gacgctcggg
     3121 ccaggcgatg acctgcggct gatagacgac cgcgctgccg ctggcgccac tgagcgtgtg
     3181 tggccagttg tcggccgccg tgacgcggtt ggcgcctgcc gccggtcgtg cagtcgtcgt
     3241 tgtcgccgcg ctaccgctgc tcgccgcgcg caaggcctgc tggcacgccg gcgaggtata
     3301 catcgcattc tcctgcaggc aggacatcgc cgccgcaccg ccagtcggca ccccggcaca
     3361 gttcgcctga taatcggcgc ggcacgcctg acggatggcg ttgacctgcg ccgaaccggg
     3421 ttgctgtgcc gccgccggca gggccaggct gacgagccct accgccaggg cagcgcgcag
     3481 cccctgacga aggcgttgga aagtgtcctg ctggatcatc ccgtattccc ctccatgttg
     3541 atatccctcg acgctgccgc cagcatccgg tcaggctatt gtaacggctg cggatcaagg
     3601 tggtggctgt tcgaagggcc agacgcaagc ctcggggact cggcgtcagg gcgcgatgg
//
LOCUS       DAOSIT010000198         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22301_length_3651_cov_8.906841, whole genome shotgun sequence.
ACCESSION   DAOSIT010000198 DAOSIT010000000
VERSION     DAOSIT010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22301_length_3651_cov_8.906841"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLL14_05980"
     CDS             <1..518
                     /locus_tag="PLL14_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOG03507.1"
                     /translation="VFAAILENNLAQAPVFVHRDYHSRNLMVTADRSAQFPANPGVLD
                     FQDAVYGPLTYDLVSLYRDAYIEWPEDQELDYVIRYWEAARQAGLPVHADFHDFYRDY
                     EWMGAQRQLKVLGIFARLCHRDGKSDYLKDMPRVLGYLCRTCQRYAELRPLARLLDTL
                     GERQNPIGFTF"
     gene            518..1210
                     /locus_tag="PLL14_05985"
     CDS             518..1210
                     /locus_tag="PLL14_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOG03508.1"
                     /translation="MKAMILAAGRGERLRPLTDSTPKPLLHAGGKALIVWHLERLAAA
                     GWREVVINHAYLGEQIEAALGNGDSFGLRIAYSPEPPGALETAGGIAAALPLLGEHPF
                     LTVNGDIWCDWDFSRARDVAQILDERLAHLVLVSNPPHHPGGDFRLAGKNVQLAVGGS
                     SDALTYAGIGVFRPEFFAGIARGTAMKLRPLLDTGIGLGMISGERHAGRWVDVGTPER
                     LAALDRELARAT"
     gene            1207..2514
                     /locus_tag="PLL14_05990"
     CDS             1207..2514
                     /locus_tag="PLL14_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOG03509.1"
                     /translation="MNHQPYSDRRQRVLARLGDGIAVIPTAAERLRNRDSHYPYRFDS
                     YFWYLSGFPEPEAVIVLVGGKEPRAILFCREKNEERETWDGFRYGPETAGNAFGFCEA
                     HPIASLDQKLPELIANRSILWHSLGHDPEWDGRITRALNAVRAESRSGTRAPGEIRDL
                     RGLLDQMRLTKDAHEIELMRRAAEIASAGHARAMRACRPGMAEYELEAELSYEFRRRG
                     ADGHAYTPIVAGGINACILHYVENNRLLAGHSLVLIDAGCELSGYASDITRTFPVGGR
                     FSPEQREVYEIVLAAQAAAIAAVRPGASFMAYHQAALRVLTQGLIDLKVISGSVDGAI
                     ESEAYKPWYMHRTGHWLGLDVHDVGDYRAGTADDDWIRLEPGMVLTVEPGLYFRPATP
                     VPMHLHGIGVRIEDDVLVTAEGCTVYTNAPRTVVEIEEVMRHG"
     gene            2507..>3651
                     /locus_tag="PLL14_05995"
     CDS             2507..>3651
                     /locus_tag="PLL14_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG03510.1"
                     /translation="MAESVDRVDIAIIGAGPVGMTLALALAGGPYKVLLIDSRRRGAW
                     AADPRALALSHGTRQILEHLGAWNAEAATAIETIHVSQRGGFGRTLLMARDYDIPALG
                     YVMRYRELAAALDAHLDTSRLLNDSPLIDLRSSRECTGIRLASGAETRCFAASLVVHA
                     EGTPGNDADITVRDYRQHAVVAEVRPAGGHAHRAWERFTPDGPLALLPLAQDYSVVFS
                     VPPDQATQLLETDDDRFLAALHAHFGGRVRFTAAGPRARFPLALRIRQQLTATRQVWI
                     GNAAQSLHPVSGQGFNLGLRDAWELAQTLLASAGEDPGDDAILATYARGRQFDRGGSM
                     AFTDGLVRIFSNDLAPLRFARGLGLLTLDLLPPLRHFVARRMIWGARA"
BASE COUNT          621 a         1258 c         1127 g          645 t
ORIGIN      
        1 ccgtgtttgc cgcaatcctg gaaaacaatc tggcacaagc gccggtcttc gtgcatcgcg
       61 attaccactc ccgcaacctg atggtcaccg ctgaccgttc tgcgcaattc ccggccaacc
      121 ccggagtcct cgattttcag gatgcggtgt acggtccctt gacctacgac cttgtttcgc
      181 tatatcgcga cgcctatatc gagtggcccg aggaccagga actggactat gtgatccgct
      241 actgggaagc tgcccgccag gctggcttgc cggtgcacgc cgactttcac gatttctacc
      301 gcgattacga atggatgggc gcgcagcgcc agctcaaggt gctcggcatc tttgcacgcc
      361 tttgccaccg cgacggcaaa agcgactacc tcaaggacat gccgcgcgtg ctcggctacc
      421 tctgccgaac ctgccagcgc tacgccgagt tgcgaccgct cgcgcgcctg ctcgatactc
      481 tcggcgagcg acagaatccc atcggcttca ccttctgatg aaggcgatga ttctcgcggc
      541 cggccgtggc gaacgcctgc gaccactgac cgacagcacg ccaaaaccgt tgctgcatgc
      601 cgggggcaag gcgctgatcg tctggcacct ggagcgactg gcggcagcag gctggcgcga
      661 agtggtgatc aaccatgctt atctcggcga gcagatcgaa gcggcactgg gcaacggcga
      721 ttcgtttggc ctgcgcatcg cttactcgcc cgagccgccg ggggctctgg agaccgccgg
      781 cggcatcgct gcggcgctgc ccctgctggg agagcacccc ttcctgacgg tcaacggcga
      841 catctggtgc gactgggatt tttcgcgcgc gcgcgacgtg gcgcagatac tcgacgaacg
      901 cctcgcgcac ctggttctgg taagcaaccc gccacatcat cccggcggcg atttccgcct
      961 tgccggcaaa aatgtgcagc ttgctgtcgg ggggagcagc gacgccctga cctacgccgg
     1021 catcggcgtc ttccggccgg agttctttgc cggcattgcc cgcggtacgg cgatgaaact
     1081 gcgcccattg ctcgacactg gcattggcct cggaatgatc agcggtgaac ggcacgccgg
     1141 tcgctgggtc gacgtgggga cacccgaacg tctcgccgca ctggacagag agcttgctcg
     1201 cgccacatga atcaccaacc ctattctgac cgtcgccagc gcgtgctggc ccgcctcggc
     1261 gacggcatcg ccgtcatccc gacggcagcc gagcgcctgc gcaaccgcga ctcgcactat
     1321 ccctatcgat tcgacagcta tttctggtac ctgagcggct tcccggaacc cgaagcggtg
     1381 atcgtactgg tcggtggcaa ggaaccgagg gcgattctgt tctgccgcga aaagaacgag
     1441 gaacgcgaaa cctgggatgg tttccgctat gggcccgaaa cagcgggaaa cgccttcggc
     1501 ttttgcgagg cgcatccgat tgccagtctc gatcagaagc tgcccgagtt gatcgccaac
     1561 cgcagcatac tctggcactc gctgggccac gatcccgaat gggacggccg gatcacccgc
     1621 gccttgaacg cggtgcgcgc cgagagccgc agcggcaccc gtgcgccagg cgagattcgt
     1681 gacctgcgcg gcctgctcga tcagatgcga ctgaccaagg atgcgcacga aatcgaactc
     1741 atgcgccgcg ccgcagagat cgcctcggcc ggtcacgccc gcgccatgcg cgcctgtcgg
     1801 ccgggcatgg cggagtacga actcgaagcg gaactcagct acgagtttcg tcgccgaggc
     1861 gccgacggcc atgcgtacac cccgatcgtc gccggcggga tcaatgcctg catcctgcac
     1921 tatgtcgaaa acaaccgcct gctggccggt cattcgctgg tgctgatcga cgccggctgc
     1981 gaactctccg gctacgcctc ggacatcacc cgcacgttcc cggtcggcgg ccgcttcagt
     2041 ccggaacaac gcgaggttta cgagatcgtc ctcgccgcgc aggcggcagc gattgccgca
     2101 gtgcgtccag gcgcctcgtt catggcctat caccaggcgg ccctgcgcgt ccttacgcaa
     2161 ggattgatcg atctcaaagt gatctccggc agtgtcgacg gcgccattga aagcgaggcg
     2221 tacaaaccct ggtacatgca ccgcaccggt cactggctgg gtctcgacgt ccacgacgtt
     2281 ggcgattacc gcgccggcac tgccgacgac gactggatca ggctggagcc cggcatggtg
     2341 ttgaccgtcg agcccggcct ttacttccgt ccggcaacgc cggtgccgat gcatctgcac
     2401 ggcattggcg tgcgcatcga ggacgatgtg ctggtcaccg ccgagggctg cacggtgtac
     2461 accaacgcgc ccaggacagt tgtcgaaatc gaagaggtga tgcgccatgg ctgagtccgt
     2521 cgaccgggtc gacatcgcca tcatcggtgc cggaccggta ggcatgaccc tcgccctggc
     2581 gctggccggc ggcccgtaca aggtgctgct gatcgacagc cgccgacgcg gtgcctgggc
     2641 tgctgaccca cgcgctctgg ctctctcgca tggcacccga cagattctcg agcatctcgg
     2701 cgcctggaac gccgaggcgg ccacggcgat cgagaccatc cacgtctcgc agcgcggcgg
     2761 ttttggccgc acactcctca tggcccgtga ctacgacatt cccgccctgg gctacgtgat
     2821 gcgttaccgt gagctcgcgg ccgctctcga tgcccacctt gacacgtcac ggctgttgaa
     2881 cgactcgccg ctgatcgacc tccgcagcag ccgcgagtgc accgggatca gactcgccag
     2941 cggtgccgag acacggtgtt tcgctgccag cctcgtcgtc catgccgaag gcactcccgg
     3001 caacgacgcc gacatcacgg tgcgtgatta ccggcagcac gccgtggtcg cagaagtccg
     3061 tcctgccggg ggacatgcgc atcgcgcatg ggagcgcttt acgcccgacg gcccgctggc
     3121 cttgctgccg ttggcgcagg actattcggt ggtgttctcg gtaccgccgg atcaggcaac
     3181 gcaactgctg gagaccgacg acgatcgctt cctggccgcg ctgcacgccc acttcggcgg
     3241 ccgcgtcagg ttcaccgccg ccggtccacg cgcccgcttc ccgctggcac tgcgtattcg
     3301 ccaacaactc acggcaaccc gccaggtatg gatcgggaac gccgcgcaaa gcctgcaccc
     3361 cgtgtccggt cagggcttca acctcggtct gcgtgacgcc tgggaactcg cccagacctt
     3421 gctcgcatct gccggcgagg accctggcga cgatgccatc ctggcaacct acgcccgcgg
     3481 ccgccagttc gaccgaggcg gcagcatggc gtttaccgat ggcctggtac gtatcttttc
     3541 caacgacctg gcgccactgc ggtttgcgcg cggcctgggt ctgctgaccc tggacctgtt
     3601 gccaccttta cgccacttcg tcgcccgacg catgatctgg ggagccaggg c
//
LOCUS       DAOSIT010000199         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22585_length_3613_cov_11.126194, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000199 DAOSIT010000000
VERSION     DAOSIT010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22585_length_3613_cov_11.126194"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLL14_06000"
     CDS             complement(<1..218)
                     /locus_tag="PLL14_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG03511.1"
                     /translation="MPTGHKRQGSALEANRRDELLRAAARLFVEKGFDATTTRDIAEA
                     VGMRSGSPFYHFRSKQDLLKAAMIEGLEA"
     gene            complement(224..2248)
                     /locus_tag="PLL14_06005"
     CDS             complement(224..2248)
                     /locus_tag="PLL14_06005"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HOG03512.1"
                     /translation="MPRHAYPHLLAPLKLGFTTLKNRVLMGSMHTGLEEAPQGFARLA
                     AFYAERARGGVGLIVTGGIAPNQDGVVMPGAAVLENEAQAENHRQITDAVHQAGGKIA
                     MQILHTGRYAYHRGAVAPSAAQAPISPIRPRELSEDDIERTLNDYARCAALAQIAGYD
                     GVEVMGSEGYLINQFIAQQTNFREDRWGGSFENRCRFAVESVRRVREATGENFIVIYR
                     LSMLDLVEGGSTWDEIVALAKAIEAAGATLINTGIGWHEARIPTIATMVPRAAFVWVT
                     KRLMGQVGIPLITTNRINTPAVAEEVIATGCADMVSMARPFLADADFVNKAAAGRADE
                     INTCIACNQACLDEIFEGRLTYCLVNPRACRETELIIEQASAPKRIAVVGAGPAGMAC
                     ALTAAERGHWVTLFDAAPEIGGQFNLARRIPGKEEFGETLRYFDRRLQKAGVSLELGT
                     CCDAASLVEAGFEHVVLATGIVPRRLELPGIEHEKVIAYTDLIEGYRIAGERVAIIGA
                     GGIGFDVAEFLTHADDDRDELERFQGEWGIDPDFANRGGLKPPSDEAVRRQVWLLQRK
                     TSKVGDGLAKTTGWIRRTLLKKRGVQMLSGVSYERIDDAGLHIIVDGQQQCLPVDHVV
                     VCAGQEPRRDLEEGLRAAAVPLSLIGGADEARELDAKRAIDQGTRLAATL"
     gene            complement(2456..2893)
                     /locus_tag="PLL14_06010"
     CDS             complement(2456..2893)
                     /locus_tag="PLL14_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03513.1"
                     /translation="MEKIIHRYSAHFPRWCQAFGDHVPDPGGEARAVEWLIGADCVGV
                     ILLPEIRHLLMHELLGQKAPELEFRQRSVRLNRHDYHEVTVLGHPGYAALRELLLGPE
                     EAHMYLTYHLIYPPGTRIITLSQKSPLPLLYKEMAPLAISVFE"
     gene            complement(2907..3302)
                     /locus_tag="PLL14_06015"
     CDS             complement(2907..3302)
                     /locus_tag="PLL14_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG03514.1"
                     /translation="MLVAAIMKRSVITISPLATVREALMMMKERDVKSLVVEKRHSGD
                     AYGLIAYRDVARAVIAEDGDIDLLNVYDIAQKPALQVSQHLEIRYLARLMIQYAVKSV
                     LVIDNNELQGFVSDTDVVSSLIDRAASGR"
     gene            complement(3354..>3613)
                     /locus_tag="PLL14_06020"
     CDS             complement(3354..>3613)
                     /locus_tag="PLL14_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03515.1"
                     /translation="TILKGKGLRHNERKTFLGLGLERKESLLLCVVEKQLAMRILRSV
                     KVQMKLDLPGGGLGFSLPLGSVIGIGLEQMLAFRREVEEDL"
BASE COUNT          628 a         1134 c         1166 g          685 t
ORIGIN      
        1 gcctccagcc cttcgatcat cgccgccttg agcaggtcct gcttgctgcg gaaatggtag
       61 aaggggcttc ccgagcgcat cccgaccgct tcggcgatat cacgtgtggt ggtggcgtcg
      121 aagcccttct cgacgaacag acgggcagcg gcgcgcagta actcgtcgcg gcggttcgcc
      181 tcgagcgcgc tcccctggcg cttgtggccc gtcggcacgc ggctcagagc gtcgccgcca
      241 gacgcgtgcc ctggtcgatg gcgcgcttgg cgtcgagttc gcgggcctcg tctgcaccac
      301 cgatcagcga aagcggtacc gcggccgcgc gcagaccttc ttcgaggtcg cggcgcggtt
      361 cctggccggc acagacgacg acatgatcga ccggcaggca ctgctgttga ccgtcgacga
      421 tgatatgcag gccggcatcg tcgatgcgct cgtagcttac ccccgagagc atctgcacgc
      481 cacgcttctt gagcagggtg cggcgaatcc agccggtggt cttggccagc ccgtcgccaa
      541 ccttggacgt cttgcgctgc agcagccaca cctgtcgccg tacggcttcg tcgcttggcg
      601 gcttgaggcc accacgattg gcgaagtctg ggtcgatacc ccactcgccc tgaaaacgct
      661 cgagttcgtc ccgatcgtcg tcggcatggg tcagaaactc ggcgacgtca aaaccgatgc
      721 cgccggcgcc gatgatcgcc acccgttcgc cggcgatccg ataaccctcg atcaggtcgg
      781 tataagcgat caccttttcg tgctcgatgc ccggcagttc cagccgacgc ggtacgatgc
      841 cggtggccag gacgacgtgc tcgaagccgg cttcgacgag gctggcggcg tcgcaacagg
      901 tgccaagttc gagcgaaacg ccggccttct gcaggcggcg atcgaaatag cgcagcgttt
      961 cgccgaactc ttccttgccg gggattcggc gcgccagatt gaactgaccg ccgatttccg
     1021 gagcagcatc gaaaagggtg acccaatgcc cacgttcggc cgcggtcagc gcgcaggcca
     1081 tgcccgccgg gcctgcgccg acgacggcga ttctcttggg agccgaggcc tgttcgatga
     1141 tcagttcggt ttcccggcag gcgcgtgggt tgaccaggca gtaggtcagc cggccttcga
     1201 aaatctcgtc caggcaggcc tggttgcagg caatgcaggt gttgatttcg tcggcgcgcc
     1261 cggcagccgc cttgttgacg aaatcggcat cggcaaggaa cgggcgtgcc atcgaaacca
     1321 tgtcggcaca gccggtagcg atcacttcct ctgccaccgc cggggtgttg atgcggttgg
     1381 tggtgatcaa cgggatcccg acctgtccca tcaggcgctt ggtgacccag acgaaggccg
     1441 cgcgcgggac catcgtggca atcgtcggga tgcgcgcctc gtgccagccg atgccggtgt
     1501 tgatcagcgt tgcaccggcc gcttcgatgg ccttggccag agccacgatt tcgtcccagg
     1561 tgctgccgcc ttcgacgagg tcgagcatcg acagacggta gatgacgatg aagttctctc
     1621 cggtggcctc gcgcacacgc cgcaccgact cgacagcgaa gcggcagcgg ttctcgaaac
     1681 tgccgcccca gcggtcctcg cggaaattgg tctgctgggc gatgaactgg ttgatcagat
     1741 aaccttccga tcccattacc tcgacgccgt catagccggc gatttgcgcg agcgcggcgc
     1801 aacgggcgta gtcgttgagc gtgcgctcga tgtcgtcctc gctgagttcg cgggggcgga
     1861 tcggcgagat tggcgcctgt gctgccgagg gcgcgacggc gccccgatgg taggcgtaac
     1921 ggccggtatg caggatctgc atggcgatct tgcctcccgc ctggtggacc gcgtcggtga
     1981 tctgccggtg gttctcggcc tgcgcttcgt tttccagcac ggccgcaccg ggcataacca
     2041 cgccgtcctg gttgggggca atgccgccgg tgacgatcaa acccacgcca ccacgcgccc
     2101 gctcggcgta gaaggcggca aggcgggcga aaccctgtgg cgcttcttcg aggccggtgt
     2161 gcatcgaacc catcaggaca cggttcttca gcgtcgtgaa gccgagtttg agcggggcga
     2221 gcaggtgtgg ataagcgtga cgcggcatgc tggaggtctc ctgagacgaa tggggcgttc
     2281 ggtgggtgat caatgattaa gcaagcaatt gcttgttgga ttatccggga ttgaccatga
     2341 acctgcaagt gtattgcagg agccctgtca cgtcgctggt caaaggcttg ccggggcgcg
     2401 atcgggagcg gcggctgcgt gttctgcagc cggtcctatg cctagagcgc gcacatcact
     2461 cgaaaacgct gattgcgagt ggtgccatct ccttgtaaag gagtggcagc ggtgattttt
     2521 gcgagagggt gatgatccgg gttcccggcg ggtagatcag atggtaggtc aggtacatgt
     2581 gcgcctcttc cggtccgagc aggagttcac gcagggccgc ataaccggga tggccgagca
     2641 ccgtcacctc gtggtaatca tgtcggttga ggcgcaccga acgttgccgg aactcgagtt
     2701 cgggtgcttt ctgcccaagc aactcgtgca tcaacaggtg ccgaatctcg ggcagcagga
     2761 tcacgccaac gcagtcggcg ccgatcagcc actcgaccgc ccgcgcctcg ccaccggggt
     2821 ccggcacatg atcgccaaac gcctgacacc agcgcgggaa atgcgccgag tagcggtgaa
     2881 tgatcttttc catcacactc cagggattag cggccgctgg cggcgcggtc gatgaggctg
     2941 ctcaccacat cagtgtccga gacgaagccc tgcaattcgt tgttgtcgat caccagcacg
     3001 ctcttcaccg cgtactggat catcagccgc gccaggtaac ggatttcgag gtgctgcgaa
     3061 acctgcaggg ccggcttctg agcgatgtcg tagacattga gcaggtcgat gtcgccgtct
     3121 tcggcgatca ccgccctggc cacgtcgcga taggcgatca ggccgtaggc gtcgccggaa
     3181 tgacgctttt cgacgaccag cgacttgacg tcgcgctcct tcatcatcat cagcgcttca
     3241 cgcacggtgg ccagcgggct gatggtgatg acgctccttt tcatgattgc ggcaaccaac
     3301 atggcaagaa ctccttcagt cagtaatcca gcggcgcccg ctggtcgggg tgatcacagg
     3361 tcttcctcga cttcgcggcg aaaagccagc atctgctcca gtccgatgcc gatcacgctg
     3421 cccagaggca gcgagaagcc caggcctccg ccgggcaggt caagcttcat ttgcaccttc
     3481 actgacctga ggatgcgcat cgccagctgc ttctcgacca cgcacaacag cagcgattcc
     3541 ttgcgctcca gcccgagccc gagaaaggtc ttgcgttcgt tgtgccgcag acccttgccc
     3601 ttgaggatgg tga
//
LOCUS       DAOSIT010000200         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22617_length_3609_cov_10.039674, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000200 DAOSIT010000000
VERSION     DAOSIT010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22617_length_3609_cov_10.039674"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1174
                     /locus_tag="PLL14_06025"
     CDS             101..1174
                     /locus_tag="PLL14_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG03516.1"
                     /translation="MTETSDPQLQLKKRARRRLVGAVAFAGLAAVVLPMVMDEEPKQQ
                     VQDVQIRIPGQDQVPFKPKELAAKATSATSEAVVKSVPDGEPPSNAAGGDVPPKVAVP
                     AAKVAAVAVPKAGEKPAEKKVAKPAEKPPEKAAEKKVVKPAEKVPEKTAEKKSPKLPE
                     AQPERFVEKKTAKPPEALPEKAAEKKAAKPPEALPEKVVKKEAGKPADRVPEKPLDKK
                     VEKPAEKVVEKAAEKAPVKKVDKPAEKSPESSPEDTGKSAAEEAPHTAAPPTSKSSEA
                     PQAKSGGQQVIVIGAFTNPENVKQLQGKISSAGVNTYTEVLDSPDGKKTRVRAGPFPN
                     REAADKALEKLKRIGVSGVVAGR"
     gene            1174..1662
                     /locus_tag="PLL14_06030"
     CDS             1174..1662
                     /locus_tag="PLL14_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOG03517.1"
                     /translation="MTVFDYFVVMIVTASLLLGMWRGVVGEIIALVAWVLAFFAARWW
                     GAHAAQWFTAIADPTLRVVAGWVAVFVSVLIAMALLRLAVRGLLKALGMTLSDRLLGI
                     VFGVVRGLVLVLVLVALGGMTALPKEKWWSEAYFSAPLETAVLASKPWLPSDVAKRIR
                     FG"
     gene            1671..3197
                     /gene="purF"
                     /locus_tag="PLL14_06035"
     CDS             1671..3197
                     /gene="purF"
                     /locus_tag="PLL14_06035"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG03518.1"
                     /translation="MCGILGVVATTPVNQLLYDGLMVLQHRGQDAAGIVTVEDDAFHM
                     HKGSGLVRDVFRTRNMRALPGNMGIAHCRYPTAGSAFDSALSQPFYVNSPFGIVLGHN
                     GNLTNAEQLKEEMFRQDLRHINTNSDSEVLLNTLAHELQATARGCRLDLDTIFAAVAA
                     VHRRCRGAYAVVAMIAGHGLLAFRDPFGIRPLVFGTNETAQGTEYLFASESVALDTLG
                     FQVLRDVDPGEAIFIDLEHRMHQRQCASNAVLSPCIFEFVYLARPDSVIDGVSVYEAR
                     LSMGERLADKLVKRIAVEQIDVVIPIPDSSRPSAMQLAQRIGVPYREGFVKNRYIGRT
                     FIMPGQATRARSVRQKLNTVAQEFKGKRVLLVDDSIVRGTTSREIVEMARAAGALKVY
                     FASASPPVRYPNVYGIDMPTRGELIATGRTGEEIGCEIGADALVYQDLEALKASIRDL
                     KPSLCHFDTSCFDGSYVTGDVSAEYLTAIERAREGHHASTKDERRASKQLQLNLMSEG
                     "
     gene            3203..>3609
                     /locus_tag="PLL14_06040"
     CDS             3203..>3609
                     /locus_tag="PLL14_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOG03519.1"
                     /translation="MRHSQDYSIETLAVRAGQERSQFNEHSEALYLTSSFVFTSAAQA
                     AARFSGEDEGNVYSRFTNPTVTAFQDRLAALEGAEACVATASGMSAILSLVMALCSSG
                     DHIVASTGLFGATQQLLGTLMPRFGIQTSFVSQT"
BASE COUNT          723 a         1049 c         1156 g          681 t
ORIGIN      
        1 gaattgtcgt ctttggatcg ttctacacgg tcgcggccgt catgcgcagc atgcaaagtg
       61 gtcgttgacc ccagcgagac gactggattc cacgctacca atgactgaaa catcagaccc
      121 gcaactgcaa ctgaaaaaac gggctcgtcg ccgcctcgtc ggcgcagttg cgtttgccgg
      181 gctggccgcc gtagttctgc cgatggtgat ggacgaggag cccaaacagc aggtgcagga
      241 tgtccagatt cgcattcccg gacaggacca ggtgccgttc aagcccaagg aactcgctgc
      301 caaggcgaca tctgcgacgt cggaggcggt ggtgaagagc gtgccggatg gcgagccgcc
      361 gagcaacgct gccggcggcg acgtcccgcc aaaggtggcc gtgccggcag ccaaggtggc
      421 cgctgtcgct gtacccaagg caggtgaaaa gcctgcagaa aagaaggtcg ccaagccggc
      481 ggagaagccg cctgaaaagg cagccgagaa gaaagttgtc aagccggcgg aaaaagtacc
      541 cgagaaaacg gccgaaaaga agtcccccaa gctgcctgag gcacagcccg agaggtttgt
      601 ggaaaagaag acggcaaaac cgccggaagc gctgcccgag aaggcggctg aaaagaaggc
      661 ggccaagcca ccggaggcgt tgccggagaa ggtggtcaag aaagaggccg gcaagcccgc
      721 agaccgggtg ccggagaaac cgctcgacaa gaaggttgaa aaaccggctg aaaaggtcgt
      781 cgagaaggca gcagagaagg cgccggtgaa gaaagtcgac aagcccgcgg agaagtcgcc
      841 cgagtcatcc ccggaagata cgggcaagtc agccgcggag gaggcgccgc ataccgcagc
      901 gcctcccacc agcaagtcgt cggaagcacc gcaggcgaaa tccggcggac agcaggtgat
      961 cgtgatcgga gccttcacta atcccgaaaa cgtcaaacaa ttgcagggca agatcagcag
     1021 tgccggtgtc aatacctata ccgaggttct cgattcaccg gatggcaaga agactcgcgt
     1081 gcgtgccgga cccttcccga atcgcgaggc cgccgacaag gcgctcgaaa aactgaagag
     1141 gattggcgtt agcggagtcg tcgccggcag gtaatgacag tctttgatta cttcgtggtg
     1201 atgatcgtca cggcttcgct tctgctgggc atgtggcgag gcgtggttgg cgagatcatc
     1261 gcactggtcg cctgggtgct ggccttcttt gcggcgagat ggtggggggc acacgccgcg
     1321 cagtggttca cggcgattgc cgacccgaca ctgcgcgtgg tcgccggctg ggtggccgtg
     1381 ttcgtgtcgg tgctgatcgc catggcgctg cttcgcctgg cggtgcgtgg cctgctcaag
     1441 gcgctgggaa tgaccctcag cgaccgcttg ctgggaattg tcttcggtgt ggtccgtggc
     1501 ctggtgctcg tgctggttct cgtggcgctg ggtggcatga cggcgctgcc aaaggaaaag
     1561 tggtggagtg aagcttactt ctctgcccct ctcgaaacag ccgtgctcgc cagcaagccc
     1621 tggctgcctt ccgacgtggc taaacgaatc aggtttgggt gacaaagact atgtgcggaa
     1681 ttctcggagt tgttgccacc acgccggtca accagttgct ttacgacggg ctgatggtcc
     1741 tgcaacatcg tggtcaggac gccgccggta tcgtcaccgt cgaggacgat gccttccaca
     1801 tgcacaaggg ctccggactg gttcgcgacg tgttccgtac gcgcaatatg cgagccttgc
     1861 cgggtaacat ggggattgcc cattgtcgct atccgaccgc cggttcggcc tttgactcgg
     1921 cgctatcgca gcccttctac gtcaactcgc cgttcgggat cgtgctcgga cacaacggca
     1981 acctgaccaa cgccgagcaa ctcaaggagg agatgtttcg tcaggatctg cgacacatca
     2041 acaccaactc cgattcggaa gtgctgctga acactctggc gcatgaattg caggccacgg
     2101 cccggggctg tcggctcgac ctcgatacca tctttgctgc ggtggctgcg gtgcatcgcc
     2161 gttgccgggg agcctatgcg gtggtggcca tgatcgccgg ccacggcctg ctggcctttc
     2221 gcgatccctt tggcattcgg ccgctggttt tcggtaccaa cgagacggcg cagggcacgg
     2281 agtatctttt tgcctccgag tccgtggccc tcgataccct gggttttcag gtgctccgcg
     2341 atgtcgatcc gggtgaggcg atcttcatcg atctcgaaca tcgcatgcat caacggcaat
     2401 gtgcgagcaa cgccgtgctg tcgccctgca tcttcgaatt cgtgtatctg gctcgccccg
     2461 actcggtgat cgacggggtg tcggtgtatg aagcacgcct gtcgatggga gaacgtctgg
     2521 cggacaagct ggtgaagcgc attgctgtcg agcagattga cgtggtgatc ccgattcccg
     2581 attcgagccg cccatcggcg atgcagctcg cacaacgaat cggtgttccg tatcgcgagg
     2641 gtttcgtcaa gaaccgctac atcggccgca cgttcatcat gcccgggcag gccacgcgcg
     2701 cgcgttcggt gcgtcagaag ctgaacaccg tggcacagga attcaagggc aagcgcgtcc
     2761 tgctggttga tgattcgatc gtacgcggca cgacaagtcg cgagatcgtc gagatggcac
     2821 gcgcagcagg cgcgctcaag gtctactttg cctccgcctc accgcccgtg cgctatccca
     2881 atgtgtatgg catagacatg ccgacgcgtg gcgaactgat tgccaccggt cgtaccggcg
     2941 aagaaatcgg ctgtgaaatt ggcgccgatg ccctcgtcta tcaggatctc gaagcactca
     3001 aggcgtcgat ccgcgacctc aaaccgtctc tttgccattt tgatacctca tgcttcgatg
     3061 gttcgtacgt taccggcgat gtcagtgccg agtatctgac ggccatcgaa cgggcacgcg
     3121 aaggccatca tgcatccacc aaggacgagc gccgggcgtc caagcagttg cagttgaacc
     3181 tgatgtcgga aggctgatga gcatgcggca tagccaggat tattcgatcg agacgctggc
     3241 ggtgcgtgcc ggtcaggagc gcagccagtt caacgaacac agcgaagcgc tgtacctgac
     3301 ctcaagcttc gtcttcacga gcgcggcgca ggctgccgca cgtttttccg gggaggacga
     3361 gggcaatgtg tactcgcgct tcaccaatcc gacggttacc gcctttcagg atcgtctggc
     3421 cgcgcttgaa ggtgccgagg cgtgtgtggc cacagcctcc ggaatgtcgg ccattctgtc
     3481 gctggtgatg gcgctgtgca gcagcggtga ccatatcgtg gcttcgacag gtttgttcgg
     3541 cgccacccag cagctgttgg gcaccctgat gccgcgcttc gggatccaga ccagcttcgt
     3601 ttcccagac
//
LOCUS       DAOSIT010000201         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22677_length_3601_cov_9.514382, whole genome shotgun sequence.
ACCESSION   DAOSIT010000201 DAOSIT010000000
VERSION     DAOSIT010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22677_length_3601_cov_9.514382"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..1035
                     /gene="galE"
                     /locus_tag="PLL14_06045"
     CDS             31..1035
                     /gene="galE"
                     /locus_tag="PLL14_06045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOG03520.1"
                     /translation="MNVVVTGGTGYIGSHACVALLAAGHAVTVVDDLSNSRVEVLDRV
                     ARIAGQRPAFFPGDVRDRALLRQVFSTAQADAVIHFAGLKAVGESVAQPLRYYDCNVG
                     GAIALCEVMAEVAVKTLIFSSSATVYGDPATVPIREDFPRSATNPYGASKLMIEDILA
                     DLALADKEWRIARLRYFNPVGAHESGLIGEEPNGVPNNLLPYVAQVADGTRQQLSVFG
                     SDYPTPDGTGVRDYIHVMDLVEGHLAALNYLLANDGLLTVNLGTGCGYSVLDMIRAFK
                     TASGRPIPYALVPRRAGDVATCYADPQRAADLLGWQARRDIDQMCRDAWRWQQQRAEI
                     "
     gene            1041..1862
                     /locus_tag="PLL14_06050"
     CDS             1041..1862
                     /locus_tag="PLL14_06050"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HOG03521.1"
                     /translation="MATYAIGDIQGCFDSLQLLLEKCRFDAANDRLWLVGDLVNRGPR
                     SLDTLRFVRDLGSAAITVLGNHDLYLLMTAAGLGRRSRGDTLDEILNATDREDLLDWL
                     RHRPLCHHEGEYCMVHAGLLPQWTVAQARALAAEVEAALSGPTYLDFLANMWGSEPAA
                     WSEELEGWPRLRVIVNAMTRMRFCSPEGVMEFKTKGELSAAPDNHLPWFEVPGRRSAT
                     DVLVIGHWSALGLRVEPNLLALDSGCFWGRHLTAIRLEDRMLFQVDCSACEAENA"
     gene            1926..2303
                     /locus_tag="PLL14_06055"
     CDS             1926..2303
                     /locus_tag="PLL14_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG03522.1"
                     /translation="MWAEAFDLLQGAEQLQRRFFTLESLHSVPSWEPSVDLFENGDEL
                     RLLVALPGVNSRQVEVVLDQAGLLVRGQRPLPPAFHRAAIHRLEIPYGRFERRISLPP
                     GDFQLHEQFLEDGCLVLVLHPIE"
     gene            2313..>3601
                     /locus_tag="PLL14_06060"
     CDS             2313..>3601
                     /locus_tag="PLL14_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOG03523.1"
                     /translation="MRTDRTDANRDTRAASTSVLVIPDDAMVIVPVRNVVLFPGMILP
                     LTIGREQSILAAQQAVKTERPVGILLQRDAEIETPGSEDLCLVGSVANILRYVTLPDN
                     THVIVCQGLQRFRVAEYLHGYPFPVARIERIDEPEATDSEIEARLIQLRERALKVLQY
                     LPQVSQELANAVKGIAQPGALADLVASVTELKIADRQRVLESIDLTHRLDIVLACLLG
                     RLEVLQLSRELDERTKASIDQRQREYLLREQLKSIQKELGEGDTGNALEMQALHKAIA
                     DAQMPEEVATQANKELKRLEHTSDGSAEYSMLRAYLDWLVELPWQEPGPDRIEIADAR
                     RILDADHFGLDQIKKRIIEFLAVRKLNPSGHGPILCFVGPPGVGKTSLGQSIAKALGR
                     KFVRASLGGVHDEAEIRGHRRTYIGALPGNIIQAIRKA"
BASE COUNT          705 a         1098 c         1102 g          696 t
ORIGIN      
        1 aatacacaag gcagtaaggg aagccggaaa atgaacgtcg tagtcaccgg ggggacaggc
       61 tatatcggct cgcacgcctg cgttgcactg ctggcggcgg gtcacgctgt cacggtcgtc
      121 gacgacctgt cgaacagccg cgtcgaggtg ctcgatcgtg ttgcacggat tgctgggcag
      181 cggccggcat tctttcctgg cgatgtccgg gaccgggcgc ttctgcggca ggtcttctcc
      241 acagcccaag ccgatgccgt catccatttt gccgggctta aagcggtcgg cgaatcggta
      301 gcgcagcctc tgcgctatta tgattgcaac gtcggcgggg cgatcgcctt gtgcgaagtc
      361 atggcggaag tggcggtgaa gacgctgatt ttcagttcct cggccacggt ctacggcgac
      421 ccggccacgg taccgattcg ggaggatttc ccgcgctcgg cgaccaatcc ctacggtgcc
      481 agcaagttga tgattgaaga catccttgcc gatctggcgc tcgccgacaa ggaatggcgg
      541 attgcgcgcc tgcgttattt caatccggtc ggcgcacacg aaagcggcct gattggcgag
      601 gaaccgaatg gcgtaccgaa caacctcctg ccttacgtgg cacaggtagc cgacggaacg
      661 cgccagcagt taagcgtttt cggcagcgat tatccgacgc ccgacggcac gggagtgcgt
      721 gattacatcc atgtaatgga tcttgtcgaa ggccaccttg ctgcactcaa ttatctgctt
      781 gccaatgatg ggctactgac ggttaacctt ggcaccggtt gtggatactc ggtactcgac
      841 atgattcgtg ccttcaagac cgccagtggt cggccgatac cctacgccct ggtaccacgt
      901 cgggcaggcg acgtcgcgac atgctacgca gacccgcaac gagccgcaga tttactggga
      961 tggcaagcac gacgagatat cgaccagatg tgccgcgacg cctggcgctg gcaacaacag
     1021 cgcgcagaaa tctagctggc atggcgacct acgcaatcgg cgacattcag ggctgcttcg
     1081 actcgctgca gcttctgctt gaaaaatgcc gtttcgacgc cgcaaacgat cgcctgtggc
     1141 tggtcggcga tctggtcaac cgcggcccac gctccctgga taccctgcgc ttcgtgcggg
     1201 atctcggcag cgccgcaatt actgtcctgg gaaatcacga cctctacctg ctgatgacgg
     1261 cagcaggact tggcaggcga agtcggggtg atacgctgga cgagatcctc aatgcaacag
     1321 accgtgaaga cttgctcgac tggttgcgtc atcgcccact ttgccaccac gaaggcgagt
     1381 attgcatggt tcatgccgga ttgttgccgc agtggacggt agcccaggca cgcgcgctgg
     1441 cggcggaggt tgaggcggca ctcagcggcc ccacttacct ggattttctg gccaacatgt
     1501 ggggtagcga accagcggcg tggagcgagg agctggaggg ctggcctcgc ttgcgagtaa
     1561 tcgtcaacgc gatgaccagg atgcgcttct gctcgccaga aggcgtgatg gaattcaaga
     1621 ccaagggcga gttgtcggcg gctcccgaca atcacctccc atggttcgag gttccaggcc
     1681 gacgcagcgc caccgacgtg ctcgtcatcg gccactggtc ggcgctcgga ctacgggtcg
     1741 agcccaacct gctggcgctc gattccggtt gcttctgggg ccgccacctg accgcgatcc
     1801 gcctcgaaga ccgcatgttg ttccaggttg actgctcggc atgcgaagca gaaaacgcct
     1861 gaaccatccg gcaagctgca gataaggggg tgggctcaat gcgatcgcgt aaccggagct
     1921 tctggatgtg ggctgaggcg tttgaccttc tccaaggtgc ggaacaactg caacggcgtt
     1981 tttttacgct ggagtccctg cactccgtac cctcctggga gccatccgtc gatctgttcg
     2041 agaacggtga tgaactcaga cttctggtcg ctctgcccgg agtcaattcg cgacaggtgg
     2101 aagtcgtcct ggaccaagct ggcctgctgg tgcgaggtca gcgcccgctg ccgccagctt
     2161 tccatcgcgc cgcgatccac cgcctggaga tcccctatgg gcggttcgag cgccgcattt
     2221 cgctgccacc cggtgacttc caactgcacg aacaattcct cgaggacgga tgcctggtgc
     2281 tcgtcctgca ccccattgag tgagccaaga gcatgcgaac tgacaggaca gatgccaacc
     2341 gcgacacgcg cgcggccagc accagcgtcc tggtgattcc cgacgatgcg atggtcatcg
     2401 tcccggtgcg caatgtggta ctgtttccag gcatgatcct gccgctgacc atcggccgtg
     2461 aacagtcgat actggctgcg cagcaggccg tgaagacgga gcggccggtt ggcatactcc
     2521 tgcagcggga cgccgagatc gaaacgccag gatccgaaga tctttgcctg gtcggttccg
     2581 tagccaacat tctgcgttac gtgactctcc cggacaacac gcatgtcatc gtctgccagg
     2641 ggctgcagcg ctttcgggtc gccgaatacc tgcatggcta tcccttcccg gttgctcgga
     2701 tcgaacgcat cgacgaaccg gaggcaaccg acagcgagat tgaagcgcgc ctgatccagc
     2761 ttcgtgagcg cgcgctcaaa gtcctgcagt atctgccgca agtctcacag gaactggcca
     2821 acgcggtcaa agggattgcc cagccgggcg ctcttgccga cctggtggcc agcgttaccg
     2881 aactcaagat cgccgatcgc cagcgagtcc ttgaatccat cgacctgaca caccggctcg
     2941 acatcgttct cgcctgtctg cttgggcgtc tcgaggtgct gcagttgtca cgcgaactcg
     3001 acgagcggac caaggcgagt atcgaccagc ggcaacgcga atacctcttg cgtgagcagt
     3061 tgaagtcgat ccagaaggaa ctcggtgagg gtgatacagg caatgcactt gaaatgcagg
     3121 cactgcataa agcgatcgcc gatgcacaga tgcccgaaga agtcgcgacc caggcgaaca
     3181 aggagttgaa gcgcctggag cacacgtctg acggttcggc cgagtactcg atgctgcgcg
     3241 cctacctcga ttggctggtc gagctgcctt ggcaggagcc tgggcccgat cgtatcgaga
     3301 ttgccgacgc ccgccgcatc ctggacgccg accacttcgg tcttgaccag atcaagaagc
     3361 gcatcatcga gtttcttgcg gtacgcaagc tcaacccgag cggccacggg ccaattttgt
     3421 gctttgtcgg tccgcctggc gtcggcaaga cctcgctggg acagtcaatc gccaaagcgc
     3481 tcgggcgcaa gtttgtgcgc gcctctctcg gcggcgttca cgatgaggcc gaaatacgcg
     3541 gccatcgacg cacctatatt ggcgccctac ccgggaacat cattcaggcc atccgcaagg
     3601 c
//
LOCUS       DAOSIT010000202         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22730_length_3592_cov_9.911790, whole genome shotgun sequence.
ACCESSION   DAOSIT010000202 DAOSIT010000000
VERSION     DAOSIT010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22730_length_3592_cov_9.911790"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLL14_06065"
     CDS             <1..459
                     /locus_tag="PLL14_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03524.1"
                     /translation="SWSEYDAAVQEILDLTAGTLLVFDADLAPLKLERPERIAALRRL
                     LGAREFNKRLTIVVQNTVFVRQYSPRLMDLLAVYSPAMTIIHAPPHLDGLKDSLLIAD
                     DRHLLVRFHQDHARARLTIDDAPECAPYVLRFEEILGAGGDPLSATTLGL"
     gene            490..1404
                     /locus_tag="PLL14_06070"
     CDS             490..1404
                     /locus_tag="PLL14_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG03525.1"
                     /translation="MKRSQANLVLLAVAVIWGSAFVAQAEGMAGVGPLTFTGIRFLLG
                     TAIVAPLAWREWQILSSRGARLLPADALGVAVLGVLLLLGAALQQIGMISTTVTNAGF
                     LTALYVPLVPLLAWLILGTNPHWSVWPTSIGCLAGTWLLSGVQGVEPAVGDLWVIASS
                     VFWALHVLFVGRLAERIAAPFLVACGQFLVCGLLGLLGAGLTETITASGMRQALLPIV
                     YAGVVSVAIGFTAQVVGQRYAQAADAAIILSAETVFAALFGFLLMGDRLNASGMVGCA
                     LILTCIVVVQVLPLAPTAPALERRDGRR"
     gene            complement(1444..2202)
                     /locus_tag="PLL14_06075"
     CDS             complement(1444..2202)
                     /locus_tag="PLL14_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOG03526.1"
                     /translation="MSSDGTLRTIIETRKVDKRFGTFHALKDVSATFYEGQVTAIVGP
                     SGSGKSTFLRTLNRLETHDSGEIVIDGIVLNDNLKNLDAIRREVGMVFQSFNLFSHLS
                     ILRNVALAPMRVRKMSREAAESKALNLLERVGLKNQAAKYPVQLSGGQQQRVAIARAL
                     AMDPKVLLFDEPTSALDPEMVNEVLAVMRELAYSGITMLVVTHEMGFAREVADRIIFF
                     DEGVILEDTTPESFFSNPDHDRAKAFLSQVRHGF"
     gene            complement(2216..2974)
                     /locus_tag="PLL14_06080"
     CDS             complement(2216..2974)
                     /locus_tag="PLL14_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG03527.1"
                     /translation="MNWTRQYPGWAMFAAMIGLLLFLWGMAVPLAAAPAPIGPAAEQF
                     SDGARITVGLTLIAGTAGVLIGVLAGLGKLSSFWPLRWLCDSYVWLIRGTPLLLQILF
                     VWLAFPSIMPEGIELSDFACAAIALSLNIGAYNTECVRAGILAVPHGQVEAARALGLT
                     PLQTFMDVVLPQSMRVALPALANNLASLVKDSSLAYAISVVELTMVGYRVQAESYQPI
                     PVFITIAAIYLILTTAFTTLTAALEAQLDVGQKR"
     gene            complement(2990..>3592)
                     /locus_tag="PLL14_06085"
     CDS             complement(2990..>3592)
                     /locus_tag="PLL14_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG03528.1"
                     /translation="FEVELAEAIAKKLGLKLEWRVVPFDAQLAALKQDRFDFAIASHG
                     YTEERAKSVDFAHPHYCTGGQIAAYKDGPLTVSALTGKTVGVQLATTYADAAKKLKGL
                     KNIKTYKGDPEAFSALRAKKVDAWISDKFTVKATLDKNPDAGISAGEMVFVERVSMIL
                     RKNNKTLEDKLNQGLAEVMKDGTYKALSEKYFKEDISCRS"
BASE COUNT          599 a         1131 c         1144 g          718 t
ORIGIN      
        1 tcctggagcg aatacgatgc cgcggtccag gaaatcctgg acttgacggc cggcacgctc
       61 ctcgtcttcg atgcggacct ggcgccgctc aagctcgaac gcccggagag aatcgccgcc
      121 ctgcgtcgcc tgctcggcgc gcgcgagttc aacaagcgac tgacgatcgt cgtgcagaac
      181 accgtattcg tcaggcaata cagcccgcgg ttgatggacc tgctggcggt ctactcgccg
      241 gcgatgacga tcatccacgc gccgccgcat ctcgatggcc tcaaggattc actgctgatc
      301 gccgacgacc ggcacctgct cgtccgtttt catcaggatc acgcacgcgc cagactgacc
      361 atcgacgacg cgccggagtg cgcaccctac gtgctgcgct tcgaagaaat cctcggcgcc
      421 ggcggcgacc cgttgagtgc gacgactctg gggttgtaaa ctgccgcgtt cgatccggga
      481 cacatcatct tgaaacgctc acaagccaat ctggtgttgc tggccgtcgc cgtcatctgg
      541 ggctcggctt ttgtcgcgca ggctgagggc atggcggggg tcgggcctct gaccttcacc
      601 ggcattcgtt ttctgctcgg aaccgccatc gttgctccgc tcgcctggcg tgagtggcaa
      661 atcctgtcgt cgcgcggcgc tcgattgcta cccgccgacg cgctcggcgt cgcggtgctc
      721 ggggtgctgc tgctgctcgg cgcggccctg cagcagatcg gcatgatcag cacgacggtg
      781 accaacgccg gttttctcac cgccctgtat gtgccgctgg tgccactgct ggcctggctg
      841 attcttggga caaatccgca ctggtcggtc tggccgacgt cgatcggttg tctggccgga
      901 acgtggctgc tctccggtgt ccagggcgtc gagccggcgg tcggcgacct gtgggtgatt
      961 gccagcagcg tcttctgggc actgcatgtc ctttttgtcg gacgcctcgc cgagcgtatc
     1021 gccgcgccct tcctggtggc ctgcggacag ttcctggttt gcggcttgct cggcctgctc
     1081 ggcgccgggc tgactgaaac gattacggcc tccgggatgc gccaggcgct gctgccgatt
     1141 gtctatgccg gcgtggtttc ggtggccatc ggctttaccg cacaggtggt cgggcagcgc
     1201 tatgcgcaag ccgccgacgc ggcgatcatc ctctccgccg aaaccgtttt cgccgcgctg
     1261 ttcggcttcc tgctcatggg cgaccgtctc aacgccagcg gcatggtcgg ctgcgcgctg
     1321 attctgacct gcatcgtcgt tgtccaggtg ctgccgctgg cgccgacggc gcccgcactg
     1381 gagcggcggg acggccgccg ttaggctggg cctttcccgg tcacgcgcct gcggccgtgg
     1441 ccatcaaaaa ccgtgccgca cctgcgacag aaacgccttc gcacggtcgt ggtcggggtt
     1501 cgagaagaag ctctcgggcg tggtatcttc caggatcacg ccttcgtcga agaaaatgat
     1561 gcggtcggca acttccctcg cgaaacccat ctcgtgcgtc accaccagca tggtgatgcc
     1621 gctgtacgcg agttcgcgca tcaccgccag gacttcgttg accatttccg ggtccagcgc
     1681 cgaggtgggc tcgtcgaaca gcagcacctt cggatccatc gccagcgcac gtgcgatcgc
     1741 cacccgctgc tgttggccgc cggacagttg gaccgggtac ttggccgcct gattcttcag
     1801 accgacgcgt tcgagcaggt tgagggcctt gctttcggca gcctcgcggc tcatcttgcg
     1861 gacccgcatc ggcgccagcg cgacattgcg caggatgctc agatgcgaga acagattgaa
     1921 actctggaag accattccga cctcacggcg gatcgcgtcc aggttcttca gattgtcgtt
     1981 gaggacgatg ccgtcgatga cgatttcgcc cgaatcatgc gtttcgagac gattcagggt
     2041 acgcaggaaa gtcgacttgc cggaaccgga cggtccgacg atcgccgtca cctgtccttc
     2101 atagaaggtc gccgaaacgt ccttcagcgc atggaaagtg ccgaagcgct tgtcgacctt
     2161 gcgcgtctcg atgatcgttc gaagggtgcc gtcgctgctc atgtgatggt cctggctaac
     2221 gtttctggcc gacgtcgagt tgcgcttcca gcgctgccgt gagcgtggtg aaggccgtgg
     2281 tcaggatcag atagatcgcg gcaatggtga tgaaaaccgg gatcggttga tagctttcgg
     2341 cctgtacccg gtagccgacc attgtcagtt cgacgacact gatcgcatag gccagcgagg
     2401 aatccttgac cagtgaagcc aggttgttgg ccagcgccgg cagggccacg cgcatgctct
     2461 gcggcaacac aacgtccatg aaggtctgca gcggggtcaa cccgagggcg cgcgcggcct
     2521 cgacctgacc atgcggaacg gcgaggatac cggcgcggac gcattcggtg ttgtaggctc
     2581 cgatattcag cgacaaggca atcgccgcgc aggcgaagtc ggagagctcg atgccttcgg
     2641 gcatgatcga cgggaacgcc agccagacga agagaatctg caacagcaaa ggggtgccgc
     2701 ggatcagcca gacgtagctg tcgcacagcc agcgcagtgg ccagaaactc gacagtttgc
     2761 cgagcccggc caggacgccg atcagcacgc cggcggtgcc ggcaatcagc gtcaggccga
     2821 cggtgatccg ggcgccgtcg gagaactgct ctgccgccgg tccgatcggc gccggtgccg
     2881 ccgcgagcgg caccgccatg ccccagagga acaacagcag cccgatcatc gcggcgaaca
     2941 tcgcccaacc cggatattgc ctggtccagt tcatggatcg ctactggcct caggaacggc
     3001 aggaaatgtc ttctttgaaa tacttctcgg acagcgcctt gtaggtgccg tccttcatca
     3061 cttcggccag cccctggttg agtttgtctt cgagcgtctt gttgttcttg cgcaggatca
     3121 tcgaaacgcg ttcgacgaag accatttcac cggcgctgat gccggcatca ggattcttgt
     3181 cgagcgtcgc cttgacggtg aacttgtcgg aaatccaggc atcgaccttc ttggcgcgca
     3241 gcgccgagaa ggcttcgggg tcgcccttgt aggtcttgat gttcttcagg cccttgagtt
     3301 tcttggcggc atcggcgtag gtggtggcca gttgcacacc gacggtcttg ccggtcagtg
     3361 ccgacacggt cagcgggccg tccttgtagg cggcgatctg gccgccggtg cagtagtgcg
     3421 gatgcgcgaa atcgaccgac ttggcgcgtt cttcggtata cccgtgcgag gcaatcgcaa
     3481 aatcgaaacg gtcctgtttg agcgccgcca gctgcgcgtc gaaaggaacc acgcgccatt
     3541 cgagcttcag gccgagcttc ttggcgatgg cttcggcgag ttccacttcg aa
//
LOCUS       DAOSIT010000203         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22774_length_3587_cov_9.561155, whole genome shotgun sequence.
ACCESSION   DAOSIT010000203 DAOSIT010000000
VERSION     DAOSIT010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22774_length_3587_cov_9.561155"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLL14_06090"
     CDS             <1..665
                     /locus_tag="PLL14_06090"
                     /inference="COORDINATES: protein motif:HMM:NF027763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HOG03529.1"
                     /translation="PRIGHSGLPMRGRDRRRDAPASSRGFSLPVRQNPQPAQTSMLLA
                     FGGSLIVSLLSARAHLEAQLSIKNADNASALALSLSLHNPDRATIELATMTLLDSKHY
                     ELIRVDDPEGHPIVERVGGVDHRDAPQWFERWLPIHATPGQAKINNAWQQVGTVTVVS
                     HNHLAYATLWSSVWPTIAAMTFACLVGGSLGAIILGRLKSPLQAVIDQATAIQSIFRR
                     VR"
     gene            662..1108
                     /locus_tag="PLL14_06095"
     CDS             662..1108
                     /locus_tag="PLL14_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03530.1"
                     /translation="MTNASFRERLLGLLGEHAAETSRLWLEVSENGALRYLAAFREFC
                     RALRATDCRIGLDHFGHQFSQIGVLHDLGLDYLKVDSSFVRGLDSNRGNAAFLKGLTG
                     IAHSIGLQVLAEGVTTEQEWLVLLELGFDGGTGPAISALAAKTSGE"
     gene            1222..>3587
                     /locus_tag="PLL14_06100"
     CDS             1222..>3587
                     /locus_tag="PLL14_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOG03531.1"
                     /translation="MNARLREIPYNYTSFSDREIVIRLLGPEKWAILDELRAERVTGR
                     SARMLYEVLGDIWVVQRNPYLEDDLLANRDRRAALAEALRHRLREIGKRRQGNAKVLQ
                     LIVAAEAAVDAFEQHFRDTARLRARVRKLLARHTRRDNIAFDGLARVSHVTDATDWRI
                     EYPLVVLYPDSEEEIAPLVRGCIELGLTIIPRGGGTGYTGGAIPLTALSAVINTEKLI
                     DIGPVEELLLPGCDRPCATIRSGAGVVTARLSEAAAAAERVFAVDPTSAAASCIGGNI
                     AMNAGGKKAVLWGTALDNLAWWKMVDASGHVMEVTRHNHNFGKIHEQEVARFELRRFR
                     KDGKTLYGTPEILEIPGRRFRKAGLGKDVTDKFLAGLPGVQKEGTDGLIVAARWVLHQ
                     MPQHTRTVCLEFFGQVREAVPAIVEITDYFKPGGAGRAAGVLLAGLEHLDERYLRAVG
                     YATKAKRKAETAGRPKMVLIGDLVGDDEAAVMRAASEVVRLCNLRSAEGFIAVDAETR
                     KKFWLDRSRTAAISRHTNAFKLNEDVVIPLPRMGEYCDGIERINIELSIRNKLALCDA
                     LTAFLRGELPLHRGDAELDKELLIGERRPAALDAVAAVRNRWQWLLDHLDLPLPEAEA
                     RFADLGIAAEALRNRASDPTLFHRLQDYSLRVSWKSELKPQLEDIFDGLVYRPVLERI
                     AAIHQEILRCRLFVALHMHAGDGNVHTNIPVNSDHYEMLQTAYGVVERIMALARALDG
                     VVSGEHGIGITKLEFLTEDEIRPFREYKARVDPEGRFNKGKLLPGADLA"
BASE COUNT          650 a         1236 c         1131 g          570 t
ORIGIN      
        1 caccgcgcat tggccactcc ggattgccga tgcgcgggcg cgatcggcga cgcgatgccc
       61 cggcaagcag ccgtggcttt tcgcttcccg tgcggcaaaa tccgcagccc gcacaaacca
      121 gcatgctgct cgccttcggc ggcagcctga tcgtttcgct gttgagcgcg cgcgcgcacc
      181 tcgaagcaca gctctcgatc aagaatgccg acaacgcatc ggcactggcc ctttcgctca
      241 gcctgcacaa cccggaccgg gcaacgatcg agctggccac catgacactt ctcgacagca
      301 agcactacga gttgatccgc gtcgacgatc ccgagggaca tccgatcgtc gaacgcgtcg
      361 gcggggtcga ccatcgcgat gccccgcagt ggttcgagcg ctggctgccg atacacgcca
      421 cgcccggtca ggcgaagatc aacaacgcgt ggcaacaggt cggcacggtg accgttgtca
      481 gccacaatca tctggcctat gcgacgctgt ggagcagcgt ctggccgacg atcgcggcca
      541 tgaccttcgc gtgtctggtc ggcggcagcc tcggggcgat tatcctggga cgtctgaagt
      601 cgccgctgca agccgtgatc gaccaggcca cggcgatcca atcaatcttt cggcgagttc
      661 ggtgaccaac gcgagcttcc gggaacgtct gttgggcctg ctcggcgagc acgccgccga
      721 gacctcccgg ctgtggctgg aagtcagcga aaacggtgcg ctcagatatc tcgccgcgtt
      781 ccgcgagttc tgccgcgccc tgagggcaac ggactgccgc atcggcctgg accacttcgg
      841 ccaccagttc agtcagatcg gtgtgctcca cgatctcggc ctcgactacc tgaaggtcga
      901 ttcgagcttc gtgcgcggac tcgacagcaa tcgtggcaac gcggccttcc tcaagggcct
      961 gaccggcatt gcccacagca ttgggctgca ggtgctcgcc gagggtgtga cgaccgagca
     1021 ggagtggctg gtcctgcttg agctgggttt tgacgggggc acgggcccgg ccatttcggc
     1081 gttggcggca aagaccagcg gcgagtgaag ccggcgacgc gcccgcgtaa tccccgccgc
     1141 gaggcagctg cggcaatcgc agactgtgat gctgcaccgc agtaagctag aattcagtct
     1201 ttggcggcag ccgggaacac catgaacgca cgcctgcgcg agatccccta taactatacg
     1261 tcgttctccg accgggagat cgtcatccgc ctcctcggtc ccgagaaatg ggcgattctc
     1321 gacgaattgc gtgccgaacg cgtcaccggc cgctcggcac gcatgcttta tgaagtgctg
     1381 ggcgacatct gggtcgtgca acgcaacccc tacctcgaag acgacctgct ggccaaccgc
     1441 gaccgccgtg ccgccctggc cgaggccttg cgccaccgcc tgcgcgagat cggcaaacgt
     1501 cggcagggca acgccaaggt cctgcagctg atcgtcgccg ccgaagccgc agtcgatgcc
     1561 tttgaacagc acttccgcga caccgcccgg ctgcgcgccc gggtcaggaa gctgctggcc
     1621 cggcacaccc gccgcgacaa catcgccttc gacggcctgg cacgcgtctc gcatgtcacc
     1681 gatgccaccg actggcggat cgaatatccg ctggtcgtcc tctacccgga cagcgaggaa
     1741 gagatcgcgc cgctggtgcg cggctgcatc gaactcgggc tgacgatcat cccgcgcggt
     1801 ggcggcaccg gctacaccgg tggcgccatc ccgctgaccg cgctctcggc ggtgatcaat
     1861 accgagaagc tgatcgacat cggtccggtc gaggaactgc ttctgcccgg ctgcgaccgc
     1921 ccctgcgcga ccatccgcag cggtgccggg gtggtcaccg cgcgcctctc ggaagccgcg
     1981 gcggccgccg aacgggtatt cgccgtcgac ccgacatccg ccgccgcctc gtgcatcggt
     2041 ggcaacatcg ccatgaacgc cggtggcaag aaggccgtac tctggggaac ggcgctcgac
     2101 aatctggcct ggtggaaaat ggtcgatgcc agcgggcatg tcatggaagt gacccgccac
     2161 aaccacaact tcggcaagat ccacgaacag gaggtcgcgc gtttcgagct caggcgcttc
     2221 cgcaaggacg gcaagacgct ctacggcacg ccggagatac tggaaattcc ggggcggcgc
     2281 ttccgcaagg cggggcttgg caaggacgtc accgacaagt tcctggccgg cctgcccggc
     2341 gtacagaagg aaggcaccga cggcctgatc gtcgccgccc gctgggtgct gcatcagatg
     2401 ccgcagcaca cgcgcaccgt ctgcctcgaa ttctttggac aggtccgcga agcggtgccg
     2461 gcaatcgtcg aaatcaccga ttacttcaaa cccggtggcg ccggccgggc cgcgggcgtt
     2521 ctgctggccg gcctggaaca tctcgacgag cgttacctgc gcgccgtcgg ttacgccacc
     2581 aaggccaagc gcaaggcgga aaccgcagga cgaccgaaga tggtgctgat cggcgacctc
     2641 gtcggcgacg acgaggcggc ggtgatgcgc gcagcttccg aggtggtgcg tttgtgcaac
     2701 ctgcgcagcg ccgagggctt catcgccgtc gacgccgaga cgcgcaagaa attctggctc
     2761 gaccgctcgc gtaccgctgc catctcgcgc cacaccaacg ccttcaagct caacgaggac
     2821 gtggtcatcc cgctgccacg catgggcgag tattgcgacg gcatcgagcg catcaacatc
     2881 gaactctcga tccgcaacaa gctggcgctg tgcgacgcgc tgaccgcctt cctgcgtggc
     2941 gaactgccac tgcatcgcgg cgacgccgag ctcgacaagg aactgctgat cggcgaacgc
     3001 cgcccggcgg cgctggacgc cgtagctgcg gtgcgcaacc gctggcagtg gctgctcgac
     3061 catctcgacc tgccactgcc ggaggccgaa gcccgcttcg ccgacctcgg catcgcggcg
     3121 gaagcgctgc gcaaccgggc gtcggacccg acactcttcc atcgcctgca ggactactcg
     3181 ctgcgcgtgt cctggaaaag cgaactcaag ccgcagctcg aagacatctt cgacggcctg
     3241 gtgtatcggc cggtgctcga acgcattgcc gccatccatc aggaaatcct gcgctgccgc
     3301 ctgttcgtcg ccctgcacat gcacgccggc gacggcaacg tgcataccaa catcccggtc
     3361 aactccgacc attacgaaat gctgcagacc gcctatggcg tcgtcgaacg catcatggcg
     3421 ctggcgcgcg cgctcgacgg cgtcgtctcg ggcgagcacg gcatcggcat caccaagctc
     3481 gaattcctca ccgaggacga aatccggccg ttccgcgaat acaaggcgcg ggtcgacccc
     3541 gaaggccgtt tcaacaaggg caagctgctg ccgggcgccg acctggc
//
LOCUS       DAOSIT010000204         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22804_length_3584_cov_10.096628, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000204 DAOSIT010000000
VERSION     DAOSIT010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22804_length_3584_cov_10.096628"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..418
                     /gene="rpsJ"
                     /locus_tag="PLL14_06105"
     CDS             107..418
                     /gene="rpsJ"
                     /locus_tag="PLL14_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG03532.1"
                     /translation="MHSQKIRIRLKAFDYRLIDQSAQEIVETAKRTGAVVRGPVPLPT
                     RIERFDILRSPHVDKTSRDQFEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            532..1176
                     /gene="rplC"
                     /locus_tag="PLL14_06110"
     CDS             532..1176
                     /gene="rplC"
                     /locus_tag="PLL14_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG03533.1"
                     /translation="MSLGLVGRKVGMTRIFTDDGASVPVTVLDVSNNRVAQIKTPQTD
                     GYSVVQVTFGKRRPSRVNKPLAGHLAKAGVEAGHVLKEFPVSSAELASFKPGDQISVT
                     IFAVGQKVDVTGQTIGKGFSGAIKRHHFSSQGAAHGNSVSHQAHGSTGMAQDPGRVFP
                     GKKMAGHLGAVKRTQQNLEVVRVDAERQLLLLKGAVPGSKGSDVIVRPAVKAGA"
     gene            1177..1797
                     /gene="rplD"
                     /locus_tag="PLL14_06115"
     CDS             1177..1797
                     /gene="rplD"
                     /locus_tag="PLL14_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG03534.1"
                     /translation="MELKLINEQGQEASRLDASDLLFAREYNEALVHQVLVAYQANAR
                     SGDRAQKDRHDVRHTTKKPWRQKGTGRARAGMSSSPIWRGGGRVFPNSSGENFSHKLN
                     RKMYRAGMASILSQLVRQDRLAVVDSLTIEAPKTKLFAAKIKSMGYESVLVITDDLDE
                     NVFLASRNLPNVLVLEVQQADPVSLVRFQQVLMTKAALAKFEEILG"
     gene            1794..2096
                     /gene="rplW"
                     /locus_tag="PLL14_06120"
     CDS             1794..2096
                     /gene="rplW"
                     /locus_tag="PLL14_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG03535.1"
                     /translation="MNQERLMQVLLAPQISEKATFVADKCEQVIFRVASDATKPEVKG
                     AVELLFKVSVESVQIANVKGKHKKFGRAMGRRNNWKKAYVCLAPGSEINFVDGGGV"
     gene            2096..2923
                     /gene="rplB"
                     /locus_tag="PLL14_06125"
     CDS             2096..2923
                     /gene="rplB"
                     /locus_tag="PLL14_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG03536.1"
                     /translation="MALVKVKPTSPGRRAVIQVVNHSLHKGAPVAGLLEPQSKHAGRN
                     NNGHITTRHQGGGHKQHYRLVDFKRNKDGVPAKVERLEYDPNRTANIALLCYADGERR
                     YIVAPKGLTVGQQVSSGSEAPIKVGNTLPIRNIPVGTTIHCVEMVPGKGAQLARSAGT
                     SVQLLAREGVHAQLRLRSGEIRRVHVECRATIGEVGNEEHSLRSIGKAGGNRWRGVRP
                     TVRGVAMNPIDHPHGGGEGKTASGMHPVSPWGTKTKGYRTRRNKRTTSMIVQRRQKR"
     gene            2937..3212
                     /gene="rpsS"
                     /locus_tag="PLL14_06130"
     CDS             2937..3212
                     /gene="rpsS"
                     /locus_tag="PLL14_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG03537.1"
                     /translation="MGRSVKKGPFADAHLIKKVGVVRATGDKRPIKTWSRRSTVLPDF
                     VGLTIAVHNGKQHVPVYITENMVGHKLGEFSLTRTFKGHTSGKKAKK"
     gene            3223..3552
                     /gene="rplV"
                     /locus_tag="PLL14_06135"
     CDS             3223..3552
                     /gene="rplV"
                     /locus_tag="PLL14_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG03538.1"
                     /translation="METRAVLRGVRLSSQKGRLVADQIRGLPVDKALNILAFSPKKGA
                     AIIKKVLESAIANAEHNDGADIDELKVKTICVEKGMVLKRFTARAKGRGNRIVKPTCH
                     VFLTVGN"
BASE COUNT          791 a         1015 c         1102 g          676 t
ORIGIN      
        1 aaggcggtcg taccgttggc gccggcgtgg tcgccaaaat catcgagtag ttcggcaaac
       61 cccgggtggg gtttgccgcc ccaccgaccg ctctttggaa tacaaaatgc atagccagaa
      121 aatacgcatc cgccttaaag ccttcgacta tcgtctgatt gatcagtctg cacaggaaat
      181 cgtcgaaacc gccaagcgta ctggagccgt tgtacgcggc ccggtgccgt tgccgacccg
      241 catcgagcgc tttgacattc ttcgctcgcc gcatgtcgac aagacctcgc gtgaccaatt
      301 tgaaatccgc acgcacctgc gcctgatgga catcatcgat ccgacggaca agactgtcga
      361 cgcgctgatg aaacttgatc tgccggcagg tgttgacgtc gaaattaagt tgcagtagta
      421 ccgtccttgc ggctataatc gccgcctttc gccggacagt cttgcatttc aactgtccgt
      481 tccctcagta aaactcgccg gccaatcgta gccggttaga ggagaataag catgagtcta
      541 ggccttgttg ggcgcaaggt cggcatgacg cgcatattta ccgacgacgg cgcaagcgtc
      601 ccggtgaccg tgctcgatgt gtcgaacaac cgtgtggccc agatcaaaac gccgcaaact
      661 gacggttact ccgtcgtcca ggtgactttc ggcaaacgcc gtccatcgcg ggtaaacaag
      721 ccgttggccg ggcatctggc caaagccggc gtggaagcag ggcatgtgct gaaagagttt
      781 ccggtctctt ccgcggaact cgccagcttc aagcctggcg accagatcag tgtgaccatc
      841 ttcgctgtcg gccagaaagt cgacgtcacg ggtcaaacca ttggcaaggg tttctccggg
      901 gcgatcaagc ggcatcactt ctcatcccag ggcgcggcgc acggcaactc ggtttcgcac
      961 caggcgcacg gttcgaccgg catggcgcaa gaccccggtc gcgtcttccc gggcaagaag
     1021 atggccggtc atctcggggc ggtcaaacgt acccagcaaa acctcgaagt ggttcgtgtc
     1081 gatgccgaac gccagttgct gttgctcaag ggcgcggttc ccggctccaa ggggtcggac
     1141 gtgattgtgc gtccagcagt caaggcgggg gcataaatgg aactcaagtt gatcaatgag
     1201 caaggtcagg aagcgagtcg tctggatgct tccgacctgc tgtttgcacg cgaatacaac
     1261 gaagcgctgg tgcaccaggt gctggtcgcc taccaggcaa acgcccgcag tggcgaccgc
     1321 gcccagaagg atcgccacga tgtgcggcat acgaccaaga agccctggcg ccagaaaggc
     1381 accgggcggg cgcgggcggg catgtcgtcg tcgccgatct ggcgcggcgg cggtcgcgta
     1441 ttccccaact cgtccggcga gaatttcagt cacaaactga atcgcaagat gtaccgcgcg
     1501 ggcatggcgt cgattctctc gcaactggtt cgccaggatc gcctcgcggt ggtggatagc
     1561 ctgaccatcg aggcgccgaa gaccaagctg ttcgcggcga agatcaagag catgggctac
     1621 gagtccgtgc tggtgatcac cgacgacctc gacgagaacg tgtttctcgc ttcgcgcaat
     1681 ctgccgaacg ttctggtgct cgaagtccag caggccgatc cggtgtcgct ggtccgcttt
     1741 cagcaggtgc tgatgaccaa agctgcgctc gccaagttcg aggagatcct gggatgaatc
     1801 aagaacgtct gatgcaggtg ctgctcgcac cgcagatctc cgagaaagcg actttcgttg
     1861 ccgacaagtg cgagcaggtg atttttcgtg tcgcctcgga tgccaccaag cctgaggtga
     1921 agggtgctgt cgaactgttg ttcaaggttt cggtagagtc cgtccagatc gcgaacgtca
     1981 agggcaagca caagaagttt ggtcgtgcca tgggtcgccg taacaactgg aaaaaggctt
     2041 atgtctgtct ggcgccgggc agcgagatta atttcgtcga tggaggtggc gtctaatggc
     2101 actcgtcaaa gtcaaaccga cctctccggg tcgccgtgct gttatccagg tggtcaatca
     2161 ctctctgcac aagggtgcgc ccgtggccgg gctgcttgaa ccacaatcca agcacgctgg
     2221 ccgcaacaac aatggtcata tcacgacacg gcaccagggt ggcggacaca agcaacacta
     2281 tcgactggtg gacttcaagc ggaacaagga cggcgttccg gccaaagtgg agcgcctgga
     2341 atacgacccg aaccggacgg ccaacattgc cctcctgtgt tatgccgacg gcgagcgccg
     2401 ctacatagtc gccccgaagg gcctgacggt tggccaacag gtatcgagtg gttccgaggc
     2461 cccgatcaag gtgggaaaca ctttgcccat taggaatatc ccggtcggca cgaccatcca
     2521 ctgtgtcgag atggtgccgg gcaagggcgc ccagttggcc agatctgcgg gtacttcggt
     2581 gcagttgctg gcgagagaag gcgtgcacgc gcagttgcgc ttgcgttctg gcgagatccg
     2641 gcgggttcac gtcgaatgtc gagcgaccat cggtgaagtg ggcaacgaag agcacagcct
     2701 ccgttccata ggcaaggccg gtggcaaccg gtggcgcggt gttcgtccga cggttcgcgg
     2761 tgtggcgatg aacccgattg accacccgca cggcggcggc gaaggcaaga ccgcatcggg
     2821 gatgcatccg gtcagtcctt ggggtaccaa gacgaaaggt tatcgcactc gtcgcaacaa
     2881 gcgcacgact tcgatgatcg tgcagcgccg tcaaaaacgc taagggatag agaaccatgg
     2941 gacgttccgt aaaaaagggc ccgtttgccg atgcccatct gatcaaaaaa gtgggcgtgg
     3001 tgcgcgcgac gggcgacaag cgaccgatca agacgtggtc gcgtcggtca accgtgctgc
     3061 cggatttcgt gggtctgacc atcgccgttc acaacggcaa gcaacacgtt ccggtgtaca
     3121 tcaccgagaa catggtcggg cacaagctgg gcgagttctc gctgacccgt accttcaagg
     3181 gccacacctc tggcaagaag gccaagaagt aaaggaatga atatggaaac tcgtgcggtt
     3241 ctgcgcggcg tgcggctatc aagccagaag ggtcggttgg tagccgacca aattcgcggt
     3301 ttgcctgtgg acaaggcgct caacattctg gctttcagtc cgaagaaggg tgctgcaatc
     3361 atcaagaaag tacttgagtc ggcaattgcc aacgccgagc acaacgatgg tgccgacatt
     3421 gacgaactga aggtgaagac gatctgcgtc gaaaagggca tggtgctcaa acgcttcacg
     3481 gcgcgggcaa aaggacgcgg caaccggatc gtcaagccga cctgtcacgt ttttctgacc
     3541 gtcggcaact gaggaacaag aatgggacaa aagattcatc ctgc
//
LOCUS       DAOSIT010000205         3575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_22880_length_3575_cov_9.845170, whole genome shotgun sequence.
ACCESSION   DAOSIT010000205 DAOSIT010000000
VERSION     DAOSIT010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22880_length_3575_cov_9.845170"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLL14_06140"
     CDS             <1..873
                     /locus_tag="PLL14_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG03539.1"
                     /translation="AAVSVCQNRIAEKNFLRDNALPHAAFTVAHDEQDLRQAGPGLFP
                     GILKVARFGYDGKGQAVVGNRDEALAAFQHFKGESCVLEQKLDLECEISVVLARDEAG
                     VISTFPVAENSHRRGILDVSVVPARAADALRARAAALAGTIAERLDYVGTLAVEFFVV
                     HGQLMINEMAPRPHNSGHYTLDACVTSQYEQQVRALCGLPLGEAGAHSAAAMVNLLGD
                     LWYETGPAGQRYREPDWSRLYAFPTLKLHLYGKHHARPGRKMGHFTVLDDDPEQAREL
                     AMAARALIGIVDAG"
     gene            863..>1708
                     /locus_tag="PLL14_06145"
     CDS             863..>1708
                     /locus_tag="PLL14_06145"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG03540.1"
                     /translation="MPANVPEIDRAVALLQAGELVAFPTETVYGLGADAANPTAVGRI
                     FAAKGRPADHPLIVHLPADGYLDRWAREIPAVAWELAEAFWPGPLTLILKRAPAVSSA
                     ITGGQDSVGLRVPAHPLALALLRAYARAGGGVDGMSGIAAPSANRFGRISPTEATHVR
                     DELGDAVGLILDGGRCPVGIESTILDLTPAAGLPPRLLRPGRVTPEQIRMVIGVLPEI
                     VGHPGDDGTPRVSGSLAAHYAPTTPLRLVTPERLTEAIERLRCSGQRCAVLCRNELPD
                     DGAALA"
     assembly_gap    1709..1808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1809..>3575)
                     /locus_tag="PLL14_06150"
     CDS             complement(<1809..>3575)
                     /locus_tag="PLL14_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOG03541.1"
                     /translation="ALNAGPADFLGAGWSLHLERVRYQQQRWQSVGSLHALPVVDTLR
                     EFPEWFESFSGAVKGNGDSLRVNGEWDIGSAPAIATLPQRSRPTGRLRLWRASGDLSI
                     GALPLGLEEGTFNLLAVDGRLAGQFKLRGKRLGELAGELDAASSAAAVLDRVAPWHGQ
                     LRVSAPDLAWAGPLLGEGWQLGGRLAGAMRIEGTPAQPRLSGEWRGDDLALRVLDQAM
                     RLEKGKLLVELSGEADGDVRLVLKQLSFDSLLQPLPRALLLDPGIDAGTLTGKPGRVE
                     ATGELRAGHAEGVLTLRAERLGVMQRPDQWVLVSGDAQFRLGERVLDVLGRFRLDAGY
                     WELARSGTPQLSEDVLIKRQSTAPAKVPAPVRLLSLDLDAELGRHFHFRGAGVESRLV
                     GAVNLRSAGSGLPRATGTIRTQDGRFDAYGQKLEIERGILNFQGLIDNPGLNIRAVRG
                     NLPVEAGVEVTGTARRPVVRLVSDPEVPDAEKLSWLVLGHAPDQQRGQDSAMLLAAAQ
                     TILGGQDGGPLKAVQRGLGIDEFGISSGRLDGSGQRQTSRIASTTGFGPSDTTTGQIV
                     SVGKRLSSNMLISYDQSLTTAGS"
BASE COUNT          577 a         1267 c         1110 g          521 t
ORIGIN      
        1 gcggcggtca gcgtctgcca gaatcgcatc gccgaaaaga acttcctgcg cgacaacgcc
       61 ctgccgcatg ccgccttcac cgtcgcgcac gacgagcagg acctgcgcca ggccgggccc
      121 gggcttttcc ccggcatcct gaaggtcgcc cgctttggct acgacggcaa gggccaggcg
      181 gtggtgggca accgtgacga agcgcttgcc gctttccagc acttcaaggg cgaaagctgc
      241 gttctcgaac agaagcttga cctcgaatgc gagatttcgg tggtgctggc acgcgacgag
      301 gcgggcgtca tcagcacctt cccggttgcc gagaacagtc accgcagggg cattctcgac
      361 gtctcggtgg tcccggcgcg tgcggccgac gcactgcgcg ccagggccgc cgcactcgcc
      421 ggaacgatcg ccgagcgcct cgactacgtc ggcacgctgg ccgtcgagtt cttcgtcgtg
      481 cacggccagc tgatgatcaa cgaaatggcg ccacgcccgc acaacagcgg ccattacacc
      541 ctcgacgcct gcgtcaccag ccagtacgaa cagcaggtcc gggccttgtg cggcctgccg
      601 ctcggcgaag ccggcgcgca ttcggccgcg gcgatggtca acctgctcgg cgacctgtgg
      661 tacgaaaccg gccccgccgg gcagcgctac cgcgagccgg actggtccag gctgtacgcc
      721 tttccgacgc tcaagctgca tctctacggc aagcatcacg cccggccggg ccgcaagatg
      781 gggcacttca cggtgctcga tgacgatccg gaacaggccc gcgaactggc catggcggcg
      841 cgcgccctga ttgggatcgt cgatgccggc taacgtcccc gagatcgacc gcgccgtcgc
      901 gctgctgcaa gccggcgaac tggttgcctt tccgaccgag acagtctatg gcctcggggc
      961 tgacgcggcc aacccgacag ccgtcggccg aatcttcgcg gccaaggggc ggcccgccga
     1021 tcatccgctg atcgtgcatc tgccggctga tggctatctc gaccgctggg cgcgcgagat
     1081 cccggcagtg gcctgggaac tggccgaggc attctggccc ggcccgctga ccctgattct
     1141 caaacgcgcg ccggccgtat cgtcggccat caccggcggg caggacagcg tcggcctgcg
     1201 cgttcccgca cacccgctgg cactggcctt gctgcgagcc tatgcacggg ccggtggcgg
     1261 ggtcgacggc atgtccggca tcgccgcccc atcggcgaat cgcttcggcc ggatcagccc
     1321 gaccgaagcc acgcatgtgc gcgacgaact cggcgacgcc gtcggcctga tcctcgatgg
     1381 cgggcgctgc ccggtgggca tcgagtcgac gattctcgac ctgacacccg ccgcaggcct
     1441 gccgccgcgt ctgctgcgcc ccggccgcgt cacccccgaa cagatccgga tggtcatcgg
     1501 cgtattgccg gagatcgtcg gtcacccggg cgacgacggc acgccgcgcg tctccggctc
     1561 gctggcggcc cactatgcgc cgacgacgcc attgcggctg gtaaccccgg agcggctgac
     1621 cgaagccatc gagcgcctgc gctgcagcgg gcaacgctgt gccgttctct gtcgcaacga
     1681 acttcccgac gacggcgcgg cactggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnngc tcccggccgt ggtcagcgac tgatcgtagc tgatcagcat gttcgacgac
     1861 agccgcttgc caacgctgac gatctggccg gtcgtcgtgt cactggggcc gaaacccgtg
     1921 gtactggcga tgcgactggt ttgtcgctgg ccggatccat cgagccggcc actgctgatg
     1981 ccgaactcgt cgatacccag gccacgctgc accgccttca ggggcccacc gtcctgcccg
     2041 ccgaggatcg tctgcgcggc ggccagcagc atcgcggaat cctgcccgcg ctgctgatcc
     2101 ggcgcgtgcc cgagcaccag ccaggacagc ttctcggcgt ccggcacttc ggggtcggaa
     2161 accagccgta cgaccggccg ccgggccgtg ccggtgacct cgacgccagc ctcgaccggc
     2221 aggttgccgc gcacggcacg gatattgagg ccggggttgt cgatcagccc ctggaagttg
     2281 agaatgccgc gctcgatttc gagcttctgg ccataggcat cgaaacgacc atcctgcgta
     2341 cggatcgtac cggtcgcccg cggcaatcca ctacctgcgg agcgaaggtt caccgccccc
     2401 accagacgac tctcgacgcc tgccccgcga aagtggaaat ggcggccgag ttccgcgtcg
     2461 aggtcgagcg acagcagccg caccggtgcc ggaaccttcg ccggtgccgt gctctggcgc
     2521 ttgatcagca cgtcctcaga gagctgcggc gtaccgctcc tggccagttc ccagtagccc
     2581 gcgtcgagcc ggaagcgccc caacacatcg agcacccgct cacccagtct gaactgcgca
     2641 tcaccggaaa ccagaaccca ctgatccggc cgttgcatca cgcccagacg ttcagcgcgc
     2701 aaggtcagca cgccctccgc atgtccggca cgcagttcgc cggtcgcctc gacccgcccc
     2761 ggtttgccgg tcagggtgcc ggcatcgatg cccggatcaa gcaacagcgc gcgcggcaac
     2821 ggctgcagca gactgtcgaa gctcaactgt ttcaggacca ggcgaacgtc accgtcggct
     2881 tcaccactca attcaacgag cagctttcct ttttcgagac gcatcgcctg gtccagaaca
     2941 cgcagcgcca ggtcgtcgcc acgccattcc ccactgaggc gtggctgggc cggcgttcct
     3001 tcaatccgca tcgcaccggc cagccgcccg ccgagttgcc aaccctcgcc gagcagcgga
     3061 ccggcccagg ccagatccgg agcactcacc cggagctggc catgccaggg agcgactcgg
     3121 tcgaggacgg cagcagcgga actggcggca tccagctcgc cggccagttc gccgagccgt
     3181 ttgccgcgca acttgaactg accggcgagg cgtccatcca cggccagcag gttgaaggtg
     3241 ccctcttcca ggcccaaagg caacgcgccg atcgagagat cgccgctcgc acgccacagg
     3301 cgcaggcggc ccgtcgggcg actccgctgc ggcaaggtgg cgatggccgg ggcactgccg
     3361 atatcccact cgccgttgac acgcagcgaa tcaccattgc ccttcaccgc gccggaaaac
     3421 gactcgaacc attccgggaa ttcgcggagg gtatcgacca ccggcaaggc gtgcaggctg
     3481 ccaacgctct gccagcgctg ctgctggtaa cgcacgcgct ccaggtgcag cgaccagccg
     3541 gcgccgagga agtcggccgg gccggcattc aaggc
//
LOCUS       DAOSIT010000206         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23044_length_3554_cov_6.973421, whole genome shotgun sequence.
ACCESSION   DAOSIT010000206 DAOSIT010000000
VERSION     DAOSIT010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23044_length_3554_cov_6.973421"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2662)
                     /locus_tag="PLL14_06155"
     CDS             complement(<1..2662)
                     /locus_tag="PLL14_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOG03542.1"
                     /translation="MLANIIGWSGRNRFLVLFATLFITIAGIYAVLRTPIDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLTTAMLSVPKSKVVRGFSFFGASFVYIIFEDGTD
                     IYWARSRVLEYLNFASSRMPKGVTPSLGPDATGVGWVYQYALLAKDKTLAELRTIQDW
                     YVRYQLTKAHGVAEVASIGGFVQTYQVTVDPVKLRSYGIPLMKVAQVIRDSNRDVGGR
                     VVEMAETEYMVRGKGYLRGKSDLEMLVVKSDKGTPVLIRDIARVELVPDERRGLTELN
                     GEGEVVSGIAMARYGANALEVIHNLKAKIDEIGGGLPAGVTVETVYDRSELIHRAIDT
                     LKRTLIEEAVIVALVCIVFLMHVRSALVAILMLPVGVLIAFIAMRSLGLNSNLMSLGG
                     IAIAIGAMIDAAIVMIENAHKHLERLPEGHTTAERADAMLAACKEVGPALFFSLLIIT
                     VSFLPVFALEGQEGRLFSPLAYTKTFSMAGAALLSVTLVPVLMMLFIRGKIMPEAKNP
                     VNRFLIWVYRPIIAGIMRWKKTTIVAAVVALVASIYPASQLGSEFMPTLNEGSLLYMP
                     ASLPGMSITKAAELLQTQNKIIKSFPEVTSVYGKAGRANTATDPAPTEMFETVINLKP
                     ESEWRPGLTTDKLIAELDKALQFPGVANSWTMPIKARIDMLSTGIRTPIGIKVFGKDL
                     GEMEKVAREIEAVVKAVPGTTSAYAERITGGFYLNIEPDREQLARYGLAVGDLQDVIG
                     TALGGEMVTTTVEGRERFGVSVRYPRELRSDPQQIAREVLIPTMDGAMIPLGQLARVG
                     VAKGAPSIRTENALLSAYIFVDIRDRDIGSYVADAKKAVAEQVSFPPGYYVTWSGQFE
                     YMERAIEKMKIVIPVTLLSIFLLLYLNFKRL"
     gene            complement(2687..>3554)
                     /locus_tag="PLL14_06160"
     CDS             complement(2687..>3554)
                     /locus_tag="PLL14_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG03543.1"
                     /translation="MPGEALYQVADLSAVWVVADVFEQDIGQVRTGAKAKVRINAYPD
                     KTFEGTISYVYPTLNAQTRTVPVRVELANPGLLLKPSMFAQVELPVGAKGQVVTLPTS
                     AVIDSGARQIVLIQQGEGRFEPREIKLGGRSDNHVEVLEGVKDGEQVVVAANFLIDAE
                     SNLKAAVGGFGHAAHGAAPTDGKPAVPGQAAAKGSSHQAEGTVDGIDAAAGTISLNHG
                     PVASLKWPTMTMEFKAANASLLQGLKPGAKVAVEFVERQPGEWVITAVKPLPANQGAG
                     TPAVGANPHAGH"
BASE COUNT          635 a         1083 c         1149 g          687 t
ORIGIN      
        1 tgaggcgctt gaagttgagg tagagcagca ggaagatcga cagcagcgtc accgggatga
       61 cgattttcat cttctcgata gcccgttcca tgtactcgaa ctggccgctc caggtgacgt
      121 agtagccggg cgggaagctg acctgttcgg cgaccgcctt cttggcgtcg gcgacgtagc
      181 tgccgatgtc gcggtcgcgg atgtcgacga agatataggc tgagagcagc gcgttctcgg
      241 tgcggatcga cggcgcgccc ttggcgacgc ccaccctggc cagttgcccg agcggaatca
      301 tcgcgccgtc catggtcggg atcagcactt cgcgggcgat ctgctgcggg tcggaacgca
      361 attcgcgcgg atagcgcacg ctgacgccga agcgttcgcg gccctcgacc gtggtcgtca
      421 ccatttcgcc gccgagcgcc gtgccgatca cgtcctgcag gtcgccgacg gccaggccgt
      481 agcgcgccag ctgttcgcgg tccggttcga tgttcaggta gaagccgccg gtgatgcgtt
      541 cggcgtaggc gctggtcgtt ccgggaacgg ctttgacgac ggcttcgatt tccctggcaa
      601 ccttttccat ttcgccgaga tccttgccga agaccttgat gccgatcggc gtgcggatgc
      661 cggtggacag catgtcgatg cgcgccttga tcggcatcgt ccaggaattg gcgacgccgg
      721 ggaattgcag ggccttgtcg agttcggcaa tcaacttgtc ggtggtcagg cccgggcgcc
      781 attcggactc cggcttcagg ttgattaccg tctcgaacat ctcggtcggc gccggatcgg
      841 tcgccgtatt ggcgcgcccg gccttgccat agaccgaagt aacctcggga aagctcttga
      901 tgatcttgtt ctgcgtctgc agcagctcgg cggccttggt gatcgacatg ccgggcaagg
      961 acgccggcat gtaaagcagc gaaccttcgt tgagcgtcgg catgaactcc gagccgagtt
     1021 gagaggccgg gtaaatcgag gccaccaagg cgacgacggc agcaacgatg gtcgttttct
     1081 tccagcgcat gatgccggcg atgatcggcc ggtagaccca gatcaggaag cgattcaccg
     1141 gattcttcgc ttccggcatg atcttgccgc ggatgaacag catcatcagc accggcacca
     1201 gcgtcaccga cagcagcgcc gcgccggcca tcgagaaggt cttggtgtag gcgagcggcg
     1261 agaacagccg cccttcctgg ccttcgaggg cgaacaccgg caggaaggag acggtgatga
     1321 tgagcaagga aaagaacagc gccgggccga cttccttaca ggcggcgagc atcgcatcag
     1381 cgcgctcggc ggtggtatgc ccttcgggca ggcgttccaa gtgtttatgg gcgttctcga
     1441 tcatcacgat ggcggcgtcg atcatggcgc caatggcgat ggcaatgcct cccaggctca
     1501 tcagattgga gttcaggccc agcgatcgca tggcgatgaa agcgatcagc acgccgaccg
     1561 gcaacatcag gatggcgacc agcgcgctgc gcacgtgcat caggaagacg atgcagacca
     1621 gcgcgacgat gaccgcttcc tcaatcagcg tccgtttcaa cgtgtcgatg gcgcggtgta
     1681 tcagttcgga acggtcgtac accgtctcga cggtgacgcc ggccggcaag ccgccgccga
     1741 tttcgtcgat cttggctttc aggttatgga tgacctccag cgcgttggcg ccatagcgcg
     1801 ccatggcgat gcccgacaca acttcgcctt caccgttgag ttcggtcagg ccgcgccgtt
     1861 catcaggcac cagttcgacg cgagcgatgt cgcggatcag cactggcgtg cccttgtcgc
     1921 ttttgacgac tagcatttca agatcgcttt tgccgcgcag gtagcccttg ccgcgcacca
     1981 tgtactcggt ttcggccatc tcgacgacgc gtccgccgac atcgcggttc gagtcgcgga
     2041 tgacctgtgc caccttcatc agcggaatgc cgtaggaacg cagcttgacc gggtcgacgg
     2101 tgacctggta ggtctgaacg aagccgccga tcgaggccac ttcggcgaca ccatgggcct
     2161 tggtcagttg atagcggacg taccaatcct gaatggtgcg cagttcggcc agcgtcttgt
     2221 ctttggcgag caacgcgtac tggtaaaccc agccgacgcc ggtggcgtcc ggcccaagcg
     2281 acggcgtgac gcccttcggc atgcggctgg aggcgaaatt gagatattca aggacgcgcg
     2341 agcgcgccca gtagatgtcg gtgccatcct cgaaaatgat gtacacgaag gaggcgccga
     2401 agaacgaaaa accgcgcacc acctttgatt tcggcaccga cagcatggct gtagtgagcg
     2461 gataggtgac ctgatcctcg acgacctggg gcgcctggcc gggatactcg gtatagacga
     2521 tgacctgcac gtccgagagg tcaggcaagg catcgatcgg cgtgcgcaat acggcataaa
     2581 tgccggcgat agtgatgaat agggtggcga acaagaccag gaagcggttc cggcccgacc
     2641 atccgataat attggccaac atggggcgac tctccgtggt tctcggtcag tgaccggcgt
     2701 gcgggttggc gccgacggcc ggcgtgccgg ctccctggtt tgctggcagc ggcttgacgg
     2761 ccgtaatcac ccattcgccg ggctgccgct cgacgaactc gacggcgact ttggcccccg
     2821 gtttcagccc ttgcagcagc gatgcgttgg cggccttgaa ctccatggtc atggtcggcc
     2881 atttgaggct ggcgaccggg ccgtgattga gactgatcgt gccggctgcc gcgtcaatgc
     2941 catccaccgt accttcggcc tggtggctgg atcctttggc ggctgcctgt ccgggcactg
     3001 ccggttttcc gtccgtcggg gcggcaccgt gggcggcgtg gccgaaaccg ccgaccgctg
     3061 ccttgaggtt gctctcggca tcgatcagga agttggcagc gaccaccacc tgctcgccat
     3121 ctttgacacc ctccagcact tcgacatgat tgtcgctgcg gccaccgagc ttgatttcgc
     3181 gtggctcgaa gcgaccttcg ccttgctgga tcagcacgat ctggcgggca ccgctgtcga
     3241 ttacggcgga ggtcggcagc gtgacaacct ggcctttggc acccaccggc agttcgacct
     3301 gggcgaacat cgacggcttg agcagcagcc ccggattggc cagttcgacg cgaaccggta
     3361 ccgtgcgcgt ctgggcgttc aaggtcggat agacatagct gatcgtgcct tcgaaggtct
     3421 tgtccgggta ggcgttgatc ctgaccttgg ccttggcccc agtcctgacc tggccgatgt
     3481 cctgctcaaa cacgtcggcg accacccaga cggccgacag gtctgccact tggtaaagcg
     3541 cctcgcccgg catg
//
LOCUS       DAOSIT010000207         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23065_length_3551_cov_9.970538, whole genome shotgun sequence.
ACCESSION   DAOSIT010000207 DAOSIT010000000
VERSION     DAOSIT010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23065_length_3551_cov_9.970538"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PLL14_06165"
     CDS             complement(<1..1632)
                     /locus_tag="PLL14_06165"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239280.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOG03544.1"
                     /translation="MDPLSPSPTLSTLEQRDDFVGRHIGPSDSEISAMLDSIGATSLE
                     QLVAQTVPATIRLPNALALAEPRPEGEALAALRAIAGGNRVRHSLIGMGYADTLTPTV
                     LLRNVLENPGWYTAYTPYQAEIAQGRLEALLNYQQMVIDLTGLELANASLLDEATAAA
                     EAMTMARRVSKAKSNLFFVDSACFPQTIDVLRTRAAFFDFELVFGSPDEAAQQEVFGA
                     LFQYPNDLGEVSDLSGPIAAVKSRGGIVAVAADLMALVLLRSPGEMGADIALGSSQRF
                     GIPLGFGGPHAAFFASRDEYKRAMAGRIIGVSVDARGKTALRMALQTREQHIRREKAN
                     SNICTSQVLLANMAGMYAVYHGPQGLRRIAGRIHRLAALLAEGLRRAGISVDTRHFFD
                     TLRVHLGGHALSANKAALDAGYNLRQVSPGVLAIAINEKTSRTDVGNLIKLLTGIHAD
                     LGILDAQLQQDDPSLPPSLLRHDAILTHPVFNTHHTEHQMLRYLKKLQNRDLALDHSM
                     ISLGSCTMKLNASSEMIPITWPEFADIHPFAPLDQA"
     gene            complement(1661..2044)
                     /gene="gcvH"
                     /locus_tag="PLL14_06170"
     CDS             complement(1661..2044)
                     /gene="gcvH"
                     /locus_tag="PLL14_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG03545.1"
                     /translation="MNIPADLKYTSSHEWVRAETDGTISIGITEHAQELLGDLVFIEL
                     PGLESEFVAGQEAAVVESVKAAADVYAPVAGTVVDVNQAVVDAPETVNQDAYAAWLFR
                     MRPNTADDLATLLDAAAYQMIVDAA"
     gene            complement(2212..3300)
                     /gene="gcvT"
                     /locus_tag="PLL14_06175"
     CDS             complement(2212..3300)
                     /gene="gcvT"
                     /locus_tag="PLL14_06175"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG03546.1"
                     /translation="MQKTVLNESHRRGGARMVDFGGWEMPLHYGSQIEEHHAVRRDAG
                     MFDVSHMCAVDLDGPDAKAFLRRLIANNVDRLQVAGKALYSAMLNEAGGVIDDLIVYY
                     LDDTRYRLVVNAGTATKDLAWMAARLDEWQLAASLRQRRDGAEALAMIAVQGPNARHK
                     VWQVLPECRSACADLKPFFAATVGEYFIASTGYTGEDGYEITLPADQAEHLWEALAAA
                     GVRPCGLGARDTLRLEAGMNLYGQDMDETVSPLDAGLAWTVDLLSPRDFVGKSALLSR
                     PRQGQFLGLVLVDRGVLRAHQKVLTSAGNGEITSGTFSPTMQQSIALARLPLGVAIGD
                     DVLVAIRDKLLAARVVKPCFVRNGTIRV"
     regulatory      complement(3377..3480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(3484..>3551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT          596 a         1074 c         1229 g          652 t
ORIGIN      
        1 cgcctgatcg agcggcgcga aggggtggat gtcggcgaac tccggccagg tgatcgggat
       61 catctcgctg ctggcattga gcttcatggt gcaggaaccg agtgagatca tcgagtggtc
      121 gagcgccaga tcccggttct gcagtttttt caggtagcgc agcatctggt gctcggtgtg
      181 gtgggtgttg aacaccggat gcgtgaggat ggcgtcgtga cgcagcaggg acggcggcaa
      241 actcgggtcg tcctgctgca gttgcgcgtc gagtattccg aggtcggcgt ggatgccggt
      301 gagcagtttg atcaggttgc cgacgtcggt tcgtgaagtc ttctcattga tggcgatggc
      361 gagcacgccc ggggagacct ggcggaggtt gtagcccgcg tcgagagcgg ccttgttggc
      421 gctgagggcg tgccccccca aatggacgcg cagggtatcg aagaagtgtc tggtgtcaac
      481 gctgatccct gcccggcgca ggccctcggc gagcagggca gccaggcgat ggatgcgccc
      541 ggcgatcctg cgcagaccct gcgggccgtg gtagacggcg tacatgccgg ccatgttggc
      601 gagcagaacc tgcgaggtgc agatgttcga gttggccttt tcgcgacgaa tgtgttgttc
      661 gcgggtctgc agcgccatgc gcagcgcggt cttgccgcgg gcgtctaccg aaaccccgat
      721 gatgcggcca gccatcgcac gcttgtattc gtcccggctg gcaaagaagg ccgcgtgcgg
      781 gccgccgaaa ccgagcggga tgccgaagcg ctgcgacgat ccgagggcaa tgtcggcgcc
      841 catctctccc ggcgaacgca acagcaccag cgccatcagg tccgcggcga cggcgacgat
      901 gccgccgcgc gatttgacgg cggcgatggg cccgctgagg tcgctgactt caccgaggtc
      961 gttgggatac tggaacagcg caccgaagac ctcctgctgc gccgcctcgt cgggtgagcc
     1021 gaagaccagt tcgaaatcga agaacgccgc gcgtgtcctg agcacgtcga tggtctgcgg
     1081 gaagcaggcg gaatcaacaa agaacaggtt cgattttgcc ttgctgaccc ggcgcgccat
     1141 ggtcatcgct tcggctgctg cggtggcttc gtcgagcagc gaggcgttgg cgagttcgag
     1201 cccggtcagg tcgatcacca tctgctggta gttgagcagg gcttcgaggc gaccctgggc
     1261 gatttccgcc tggtagggcg tataggcggt ataccagccc ggattttcga gaacgttgcg
     1321 cagcagtacc gtcggggtca gggtgtcggc gtagcccatg ccgatcagcg agtgccgcac
     1381 gcggttaccg ccggcaatgg cccgcaaggc ggcgagcgct tctccttccg gacgcggctc
     1441 ggcgagtgcc agtgcgttcg gcagtcgaat cgttgccggc accgtctgcg ctacgagttg
     1501 ttccaggctg gtggcgccga tgctgtccag catggcggat atttcgctgt cgctggggcc
     1561 gatgtggcgg ccgacgaaat cgtcgcgctg ttccagggtc gagagcgtcg gggaaggaga
     1621 gaggggatcc atggcgtcgt ccggtagcgg taatcttggc ctaggcggcg tcgacgatca
     1681 tctgataggc ggccgcatcg agcaaggtcg ccaggtcatc agccgtgttc ggtcgcattc
     1741 tgaacaacca tgccgcgtag gcatcctggt tgacggtttc gggtgcgtcg accaccgcct
     1801 gattgacgtc gacgacggta ccggccaccg gggcgtagac gtcggcggcg gccttgaccg
     1861 attcgacgac tgccgcctcc tggccggcaa cgaactcgct ttcgagcccc ggcaactcga
     1921 tgaataccag atcaccaagc agttcctgcg catgctcggt aatgccgata ctgatcgtgc
     1981 cgtccgtttc ggcgcggacc cattcgtgac tgctggtgta cttgaggtcg gcagggatgt
     2041 tcatgttttc tcctgggtag tggttcaagg tgacggtcgc cgtggcgacg catggggtgt
     2101 cccgctgcaa atcctgcgag aggggcgggg agtggaaatg gtcgaggctt tcatgaccgg
     2161 cggcccgttc aagccgtcac gcgcagggcc tcacgtccgg gaagcaggca ttcagacgcg
     2221 gatcgtgccg ttgcgcacga agcagggttt gacgaccctg gcggccagca gcttgtcgcg
     2281 tatcgccacc aggacgtcat cgccgatcgc cacgccgagc ggtaaccggg ccagggcaat
     2341 cgattgctgc atcgtcggcg agaacgtgcc gctggtgatt tcgccgttgc ccgcgctcgt
     2401 caacaccttc tggtgcgcac gcaacacgcc gcggtcaacc agcaccaggc cgagaaactg
     2461 accctggcgg ggtctcgaca gcagtgcgga cttgccgacg aagtcgcgcg gcgacaacag
     2521 gtccacggtc caggccaggc cggcgtcgag gggtgaaacg gtctcgtcca tatcctggcc
     2581 atagaggttc atgccggcct cgagacgcag ggtgtcacgc gcccccagac cacagggccg
     2641 cacgcccgcc gcggcgagcg cttcccacag atgttcggcc tggtccgcgg gcagggtgat
     2701 ctcgtaaccg tcctcgccgg tatagccggt gctggcgatg aaatactcgc cgacggtggc
     2761 ggcaaagaaa ggcttgaggt cggcgcaggc agaacgacat tccggcagca cctgccagac
     2821 cttgtggcgc gcgttcggcc cctgcacggc gatcatcgcc agcgcctcgg cgccatcacg
     2881 ccgctgcctg agactggcgg caagctgcca ctcgtccagg cgggcagcca tccacgccag
     2941 atccttggtc gccgtaccgg cattgaccac gaggcggtag cgggtgtcgt cgaggtagta
     3001 gacgatcagg tcgtcgatca cgccgccggc ctcgttgagc atcgcgctgt acagggcctt
     3061 gcccgcgacc tgcaggcggt cgacgttgtt ggcgatcagg cgccgcagga aggccttggc
     3121 atcggggccg tcgagatcga ccgcgcacat gtgcgagacg tcgaacatgc ccgcgtcgcg
     3181 gcgcacggcg tggtgttcct cgatctgcga cccgtagtgc aggggcattt cccagccacc
     3241 gaaatcgacc atgcgtgcgc cgccgcgacg gtggctttcg ttgaggacag ttttctgcat
     3301 cgtatcaatc cataaggtga gtctgttgga gtcgattcga gaacgaaaaa ggggcgaact
     3361 cgactcgagt tcacccccct gtccatgtac ctgagagatt gtcgcgctga aaccgtgaat
     3421 cgcagccgct cttgccccat cggtgggcgc ctcgcgacaa gcgggcgccg ctctccagag
     3481 ttcattgcac gaacggttca tctgcctgag cgtttccggg tggattgcgc cttcggcggc
     3541 ggcgtggccg c
//
LOCUS       DAOSIT010000208         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23240_length_3529_cov_9.702073, whole genome shotgun sequence.
ACCESSION   DAOSIT010000208 DAOSIT010000000
VERSION     DAOSIT010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23240_length_3529_cov_9.702073"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /gene="dnaE"
                     /locus_tag="PLL14_06180"
     CDS             complement(<1..1682)
                     /gene="dnaE"
                     /locus_tag="PLL14_06180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG03547.1"
                     /translation="MPEIPFIHLRLHSEYSITDGIVRLNDAIARAASYGMPALALTDL
                     GNLFGLVKFYYAARSKGIKPLLGSDVCIANETDPDRPGRLLLLCRSRQGYLQLCELLS
                     RAYLAPRVRGRAEITRRMLQEVGVDGLIALSGAAAGDVGEALLQGNREQAASRARRWE
                     DLFRGAFYLEVQRYGQAQQDQLVAATADLAAELELPLVATHPIQFLERDDFKAHEARV
                     CIAEGYMLGDARRPRLYTEEQYFKSAAEMVELFADLPDALANAVEIAKRCNLQITLGK
                     SYLPDFPTPPGVTLDDFLRREAVAGLEVRLQQLFPDPAERAAQRPAYDARLQLETDTV
                     VQMGFPGYFLIVADFINWAKHNGCPVGPGRGSGAGSVVAYALGITDLDPLRYALLFER
                     FLNPERVSMPDFDIDFCQDNRWRVIEYVRRKYGAAAVSQIVTFGTMSSKAVIRDVGRV
                     LDLPYSFCDQLSKLIPVETNKPLSLAKAIAAEPQLKARIENEEEVRDLFDLAARLEDL
                     TRNVGMHAGGVLIAPGKLTDFCPVYSADGGGSVVSQYDKDDVEKVGLVKFDFL"
     assembly_gap    1703..1802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1829..2452)
                     /locus_tag="PLL14_06185"
     CDS             complement(1829..2452)
                     /locus_tag="PLL14_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOG03548.1"
                     /translation="MKSFLIANPKGGSGKSTLAINLAGHLARSGHRVMLGDVDRQQSS
                     REWLRLRPSILPTIRSWEIEPGKTARPPKGTTHVVLDTPAGLHGKALDTVVKQVNRVI
                     VPVQPSLFDILATRHFIEALLEEKAVRNEHAFVAVVGMRVDPRTRAAAELERFLAQYD
                     LPVLTHLRNTQLYVQTTLNGMTMFDLSASRAAKDLEQWQAIIEWVNS"
     gene            complement(2449..>3529)
                     /locus_tag="PLL14_06190"
     CDS             complement(2449..>3529)
                     /locus_tag="PLL14_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOG03549.1"
                     /translation="IENGEADVAFVQGGIALRTPDDDEEAGPLRSLGSVSYEPVWVFY
                     RGAQRVDKLYQLDGRRIAVGEEGSGIRGLALQLLEANGIPANSPNLLPLAGLNAAEAL
                     QQEQIDAAFIVAAQEAPVVQVMLRSPGLRVVSLSQADAYLRRFPFLSKIVLPRGVVDL
                     VRDVPPRDTVLLATTANVIVREDLHPAVVGLLMQAMTEVNGRGGFFQRAGEFPAYKDQ
                     SFPLSSDAARYYKSGPPFLQRYLPFWMAVLVERLLVMILPLLMLSLPLLKFAPALYSW
                     RVRSKIVRCYGDLKFLENDLRRHYEPARQQEYVQQLDRIENDASTLNIPLAFSDLLYT
                     LREHINLVRDELRRLAADHDKDNRE"
BASE COUNT          638 a         1108 c         1102 g          581 t
ORIGIN      
        1 aggaagtcga acttcaccag gccgactttt tccacgtcgt ccttgtcgta ctgggaaacc
       61 accgagccgc cgccatcggc agaatagacc gggcagaagt cggtgagttt gccgggggcg
      121 atcaatactc caccggcatg catgccgaca ttgcgcgtca gatcttcgag acgcgccgcc
      181 aggtcgaaca gatcccggac ttcttcctcg ttttcgatgc gcgccttcag ttgcggctcg
      241 gcggcaatcg ccttggccag cgacagcggc ttgttggtct cgaccggaat cagcttggac
      301 aactggtcgc aaaagctgta cggcaggtcg agcacgcgcc cgacgtcgcg aatgaccgcc
      361 ttcgacgaca tggtgccgaa ggtgacgatc tgcgacacgg ccgccgcccc gtacttccgg
      421 cgcacgtact cgatcacccg ccagcgattg tcctggcaga agtcgatgtc gaagtcgggc
      481 atcgaaaccc gctcgggatt caggaagcgc tcgaagagca gggcgtagcg cagcgggtcg
      541 aggtcggtaa tgcccagggc gtaggcgacg acggaaccgg cgcccgagcc gcgtccggga
      601 ccgaccggac agccgttgtg tttggcccag ttgatgaagt cggcaacgat caggaagtag
      661 cccggaaacc ccatctgtac gacggtatcg gtttccagtt gcaggcgcgc gtcgtaggcg
      721 ggacgctgcg ccgcccgctc ggcggggtca ggaaagagtt gctgcaggcg cacttcaagg
      781 ccggcgaccg cctcgcggcg gaggaaatcg tcgagcgtca cccccggtgg cgtcgggaaa
      841 tccggcaggt agctcttgcc gagcgtgatc tgcagattgc agcgcttggc gatttccacc
      901 gcgttcgcca gcgcgtcggg aaggtcggca aacaactcga ccatttcggc ggccgacttg
      961 aagtactgtt cctcggtgta caggcgcgga cggcgggcat ccccgagcat gtagccctcg
     1021 gcaatgcaca cccgcgcttc gtgcgccttg aaatcgtcgc gttcgagaaa ctggatcgga
     1081 tgcgtggcca ccagcggcaa ctcgagttcc gccgccagat cggcggtggc agcgaccagc
     1141 tgatcctgct gcgcctgccc ataacgctgc acttcaaggt agaaggcgcc gcgaaacaga
     1201 tcttcccaac gccgtgcgcg acttgccgcc tgttcgcggt tgccctgcag cagcgcctcg
     1261 ccgacgtcgc cagcggcggc gcccgacaac gcgatcaggc catcgacgcc gacctcctgc
     1321 agcatccggc gcgtgatttc ggcccgcccg cgcacgcgcg gcgcgaggta ggcgcgcgac
     1381 agcaactcgc agagctgcag gtaaccttgc cgggagcggc agagcagcaa caagcgaccg
     1441 ggacggtcgg gatcggtctc gttggcgatg cagacgtcgc tgccgagcag cggcttgatt
     1501 cccttgctgc gcgccgcgta gtagaacttg accaggccga agagattgcc gagatcggtc
     1561 agcgccagcg ccggcatgcc gtagctggca gcgcgcgcga tcgcgtcgtt caggcgaacg
     1621 atgccatcgg tgatcgagta ttcgctgtgc aggcgaaggt ggatgaacgg gatttcaggc
     1681 atcccgccat tttaccccga ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nngcggcgag gaccgcgcca gcccacggct agctgttcac ccactcgatg atcgcctgcc
     1861 actgttcgag atccttggct gcacgcgacg ccgacagatc gaacatggtc atcccgttca
     1921 acgtcgtctg cacgtagagc tgcgtattgc gcagatgggt gagcaccggc aggtcgtact
     1981 gggcgagaaa acgctcgagt tcagcggcag cgcgggtgcg tggatcgacc cgcatgccca
     2041 cgacggccac gaacgcgtgc tcgttgcgca cggccttctc ttcgagcagc gcctcgatga
     2101 agtggcgggt cgcgaggatg tcgaacagcg acggctgcac cggaacgatc acccggttca
     2161 cctgcttgac gaccgtgtcg agcgccttgc catgcaggcc tgccggcgtg tccagcacga
     2221 catgcgtggt ccctttcggt gggcgcgcgg tttttccggg ttcgatctcc caactgcgaa
     2281 tcgtcggcag gatcgatggc cgcaagcgca gccattcccg cgacgactgc tgacgatcga
     2341 cgtcaccgag catcacccga tgcccactgc gcgcgagatg gccggccagg ttgatcgcca
     2401 gcgtcgattt tccggaaccg cccttgggat tcgcaatcag gaaggatttc attctctatt
     2461 gtccttgtca tggtccgcag cgagccggcg tagctcatca cgaaccaggt tgatatgctc
     2521 acgcagagtg taaagcagat cgctgaaagc cagtgggata ttgagtgtgc tggcgtcgtt
     2581 ttcgatgcgg tcgagctgct gcacgtactc ctgctggcgg gccggctcgt agtgccggcg
     2641 caggtcgttc tcgaggaact tcaggtcacc gtaacagcgt acgatcttcg agcgcacgcg
     2701 ccaactgtac agggctggag cgaacttcag cagcggcagc gagagcatca gcagcggcag
     2761 gatcatcacc agcagccgct cgacgagcac cgccatccag aacggcagat aacgctgcag
     2821 gaaaggcgga ccggacttgt agtagcgcgc cgcgtcgctc gacagcggaa aactctggtc
     2881 cttgtacgcc ggaaactcac cggcacgctg aaagaaaccg cccctgccgt taacctcggt
     2941 catcgcctgc atcagcagac cgacgacggc cgggtgcagg tcttcgcgga caatgacatt
     3001 ggccgtcgtc gccagcagca cggtgtcccg gggcggcacg tcgcgcacca ggtcgacgac
     3061 cccgcgcggc agcacgatct tcgacaggaa cgggaagcgt cgcaggtagg cgtcggcctg
     3121 gctcaggctg accacccgca aacccggaga acgcagcatc acctgcacca ccggcgcttc
     3181 ctgcgcggcg acgatgaacg cggcgtcgat ctgttcctgc tgcagcgcct cggcagcgtt
     3241 caagccagca agcggcagca ggttggggct gttggcggga attccgttgg cttccagcaa
     3301 ctgcagggcc aggccccgga taccactgcc ctcctcgccg acggcgatgc gccggccgtc
     3361 gagctggtac agcttgtcga cccgctgcgc gccccgatag aacacccaca ccggctcata
     3421 tgagacgcta ccgagcgaac gcagaggacc cgcttcttcg tcatcgtcgg gtgtccgcag
     3481 ggcaatgccc ccttgcacga aggccacgtc ggcctcgcca ttttcgatg
//
LOCUS       DAOSIT010000209         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23334_length_3517_cov_7.684287, whole genome shotgun sequence.
ACCESSION   DAOSIT010000209 DAOSIT010000000
VERSION     DAOSIT010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23334_length_3517_cov_7.684287"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLL14_06195"
     CDS             <1..800
                     /locus_tag="PLL14_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOG03550.1"
                     /translation="GQQIDITVSSMGNAKSLRGGTLVMTPLKGADGQIYAQAQGNLLV
                     GGAGAASAGSKVVVNHLLAGRVVGGATVEREVPTALGQGSFIHYEMATTDFGTTQRVV
                     EAINREIGPGTAQAIDGRLVRVAAPEDANSRVAFLGRLESLEVRPTKTVAKVIINPRT
                     GSVVMNQTVTIDSCAVAHGNLSVIINTEQKVSQPNALAGGQTVTTTQSEIDIKQGGGS
                     LIQLKAGVNLAEVVKAINALGAGPQDLLSILQSMKAAGALRAELEII"
     gene            840..1775
                     /gene="flgJ"
                     /locus_tag="PLL14_06200"
     CDS             840..1775
                     /gene="flgJ"
                     /locus_tag="PLL14_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HOG03551.1"
                     /translation="MKSTELGVQRLAIDPAAAVDLRAKLRQDPQTGVKQAARQFEGML
                     LNLMLKSMRDASSGGGLLDSEQSRFFTAIGDQQLAQGLAAQAPLGFAGLIEKQLSRQM
                     SPATGDTASSTIDALQQSLLSQYSRRLPAATPGTAGAQRATGVPAAASTAGTSPGARG
                     FVDRVWPHATEAAAVTGVPAHFLVAHSALESGWGKHEIRAADGSPSFNLFGVKAGRSW
                     SGPSVEVQTTEFVNGEARPVRARFRVYASYSEAFRDYGTLLSKNPRFSGIIGQQDGTQ
                     FARSLQQAGYATDPMYADKLARIINGKALRQALAA"
     gene            1878..>3517
                     /gene="flgK"
                     /locus_tag="PLL14_06205"
     CDS             1878..>3517
                     /gene="flgK"
                     /locus_tag="PLL14_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOG03552.1"
                     /translation="MGSGIIGTGVSGLHAAQFGLQTTEHNIANANTPGYTRQRTIQAS
                     NPGLLTGAGFLGQGTRVATIERVYSRFLTEQVDRSQSSVSQLDSYYAQIKQIDNMLGD
                     ASAGLAPALQQFFSSVDQVAANPSQLATRQSMVSTAQALSARYQALGDQLAQMNDSIN
                     GEITGSVAEINSYAEQIATLNQQIGVAEASTGQPANDLQDSRDQLVFELNKRIKTTTT
                     RNDDGSYNVFIGSGQQLVVGSHVMSIASIPSSSDPSHLQVGLKTAAGVQELPEAVIKG
                     GSLGGLLAFRSESLDRASNELGRNAVSLALTFNAQSALGQDLLGQSQLTAAPTSFTPQ
                     FFSVSAPVVTSSSTNPPGSPAVIAEFITPPPFNSNFYTDLGSSDYRLSSDGSKVTLER
                     LSDGTKWSGVDLAAINTQLAGNPQGFALDPSPTLLAGSSYLIQPTHDAARKISVNPVL
                     AADPRLIPAAGPLRTTVGTSNTGTATISAGSVGPGYPGAVEPGFPAAADKLPLTLVYQ
                     GGTSPTLQNFPPGTRVSINGAAPTTITARTDTIPYTSGA"
BASE COUNT          707 a         1058 c         1104 g          648 t
ORIGIN      
        1 ctggccagca gatcgacatt accgtgtcgt cgatgggcaa tgccaagagc ctgcgcggcg
       61 gaacgctggt catgaccccg ctgaaaggtg ccgacggcca aatctatgcc caggcgcagg
      121 gaaatttgct ggttggcggc gcgggggctg cgtccgcagg gagcaaggtg gtggtcaacc
      181 atctcctggc gggtcgggtg gtaggcgggg ccaccgttga gcgcgaggtt cccacagcgc
      241 tggggcaggg gtcattcatc cactacgaga tggcaaccac cgacttcggg accacacagc
      301 gggtcgtcga agcgatcaat cgcgaaattg gtccgggtac ggcacaggcc attgacgggc
      361 ggctggttcg cgttgccgct cccgaggatg cgaacagccg ggtggctttc ctgggacgac
      421 tcgaaagcct ggaagtgcgt ccgaccaaga cggtggccaa agtgatcatc aacccgcgca
      481 cgggatcggt ggtgatgaat caaacggtga ccatcgattc ctgcgccgtc gcgcacggca
      541 atctttcggt gatcatcaat accgagcaga aggtcagtca gcccaacgca ctggctggcg
      601 gacagacggt gaccacgacg cagagcgaga tcgatatcaa acagggtggt ggttcgttga
      661 tccagttgaa ggcgggtgtc aatctcgccg aagtggtcaa agcgatcaat gccttgggtg
      721 ccgggcccca ggatctgctg tccatcctgc agtcgatgaa agcagcaggc gcgttgcgcg
      781 ccgaacttga aatcatctga gcacgtgcca ggctgcttac caggagccat caacctatca
      841 tgaaatccac cgaacttggc gtgcagcgcc tggccatcga tccggcagcc gccgtcgatc
      901 tgcgtgccaa actgagacag gatcctcaga ctggagtcaa gcaggcggcg cggcagttcg
      961 agggcatgct gctcaatctg atgttgaaaa gcatgcgtga tgcgagttcg ggcggcggtc
     1021 tgctggacag cgagcaaagc cggttcttta ccgccatcgg cgatcagcaa ctggcgcagg
     1081 ggctggccgc gcaagcgcca ctgggctttg ccggattgat cgagaagcaa ttgtctcgac
     1141 agatgtcccc cgcgacgggc gacacggcaa gttcgacgat cgatgcgctg cagcagtcgt
     1201 tgttgtcgca gtattcgcgc cgcctgcccg cagcaacacc tggcaccgct ggcgctcagc
     1261 gcgctaccgg tgtcccggcc gcagcgagca cggctggtac ctcacccggt gcgcgtggat
     1321 ttgtcgaccg cgtctggccg catgccaccg aggcggcggc ggtgaccggc gtgcccgcac
     1381 attttcttgt cgcgcactcc gcactggaga gtggctgggg aaaacacgag atccgggcag
     1441 ccgatggttc gcccagcttc aacctcttcg gcgtcaaggc cggacgcagc tggtcggggc
     1501 caagcgttga agtgcagacc accgagttcg tcaatggcga ggcacgaccg gtacgcgcca
     1561 ggttccgtgt ctatgcttcg tattccgagg cgtttcgtga ctatggcacg ctgctgagca
     1621 agaatccccg cttttccggg atcatcggtc aacaggatgg cacgcagttt gctcgttcct
     1681 tgcagcaggc cggctacgcg actgacccga tgtacgctga caaactggcg cgcatcatca
     1741 acggcaaggc tttgcggcag gctctggcgg cctagggggg tggcgttcct ggcgccatga
     1801 ctcgatatat ttggtttcgg aactccagtt cttgccgcgg ctgccgttga tagcgtaaac
     1861 aacgaggaaa ttagcacatg ggctcaggaa tcatcggcac cggtgtcagc ggtttgcacg
     1921 ccgctcagtt tgggctgcaa acgaccgaac acaatattgc caacgccaat acccccggct
     1981 acacccgcca gcgaacgatt caggcgagca atccgggttt gctaaccggt gccggctttc
     2041 tcgggcaggg cacgcgcgtt gcgacgattg agcgtgtcta cagtcgcttc ctgaccgagc
     2101 aggtcgatcg ctcgcagagc agcgtcagcc aactcgacag ctactacgcg cagatcaaac
     2161 agatcgacaa tatgctgggt gacgccagtg ccggtctggc gccggcgctg cagcagttct
     2221 tcagcagtgt cgaccaggtt gcggcaaatc cttcccaatt ggcgacccgc cagagcatgg
     2281 tctcgacggc gcaggcgctg agcgcacgct atcaggcact gggcgatcaa ctggcgcaga
     2341 tgaacgacag catcaatggc gagattaccg gcagcgtcgc cgagatcaat tcgtacgctg
     2401 aacagatcgc cacgctgaat cagcaaatcg gtgtcgccga agcctcgacc ggtcagcccg
     2461 ccaacgatct gcaggatagc cgggatcaat tggtgttcga gctgaacaaa cgcatcaaga
     2521 ccacaacgac gcgcaatgac gatggcagct acaacgtctt catcggcagt ggccagcaac
     2581 tggtcgtcgg ttctcatgtt atgagcattg cgtcgatacc ctcgtccagt gatccttcgc
     2641 acctgcaggt cggtttgaag acggcggcgg gtgttcagga actgccagag gccgtgatca
     2701 aagggggcag tctgggtggt ttgctggcgt tccggagcga atcgctggat cgggccagca
     2761 acgaactcgg tcgtaacgcc gtgtcgctgg ctttgacctt caatgcacag agtgcactcg
     2821 gtcaggacct gcttgggcag tcgcaactca cggccgcacc gacaagtttt acaccgcagt
     2881 tcttttcagt ttccgcaccg gtggtgacca gcagcagcac caacccgcct ggcagtccgg
     2941 cggttatcgc cgagttcatt actccgccgc cgttcaacag caacttctat accgaccttg
     3001 gcagcagcga ctaccgcctc agttcggacg ggagcaaggt cacgctggaa cgtttgtccg
     3061 acgggacgaa atggagcggg gtggaccttg ctgcgatcaa tacgcaactg gcgggcaacc
     3121 cacagggatt tgctctcgat ccatcaccaa ccctgttggc cggttcgagc tatctgatcc
     3181 agccgaccca cgacgccgct cgcaagatct cggtcaaccc ggtgctggcc gcggatccgc
     3241 gcttgatccc ggcggcaggg cccttgcgta ccacggtggg aacgagcaat accggtacgg
     3301 caacgatttc cgccggtagc gtagggccag gctatccagg ggcagtcgag ccggggtttc
     3361 cggcggctgc agacaagttg ccgctgacgc tggtctatca gggcggtacg tcaccgacgc
     3421 tgcagaactt tccgccgggc acgcgagtgt cgatcaatgg cgctgctccg acaacgatta
     3481 ccgcgcgcac ggataccatc ccatacacca gtggcgc
//
LOCUS       DAOSIT010000210         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23362_length_3513_cov_9.793233, whole genome shotgun sequence.
ACCESSION   DAOSIT010000210 DAOSIT010000000
VERSION     DAOSIT010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23362_length_3513_cov_9.793233"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1376
                     /locus_tag="PLL14_06210"
     CDS             282..1376
                     /locus_tag="PLL14_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03553.1"
                     /translation="MERQWFLESVAADGSHMTYNITHLPFCIGRDADNDLVVASPDLS
                     RHHVALTRDISGRIRLTDLNSTNGSFVNRQRIEGSCLLDENDIVHFGTTEFRMRVNLG
                     GHLRIDDMRTMLMPQGLELSARFVRHEAEFLALLEGHGVSGAAQPIVDATTKRIVAYE
                     LLGRANHPLLPISPIRLFELASALDREIDLSVAFRKFGIAAIAPRLQGFPLFANTHPK
                     ETFSEEFFDSLVSLREQRPELDLVIEIHETAVTESARLVKLAGRFRDIGVRFAYDDFG
                     AGQARLSELGDAPAHFVKFDMGLVHDIHLAGERKRRVVGDLVKLVLDLGSVPLAEGIE
                     QEAEAQICREMGFQLIQGFLTGRPIPVENL"
     gene            complement(1342..>3513)
                     /locus_tag="PLL14_06215"
     CDS             complement(1342..>3513)
                     /locus_tag="PLL14_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG03554.1"
                     /translation="SVSIREAIDDVQFTLILAGFLVILVILLFLRNLSATVIPALALP
                     ISVVGTFGAMSLLGYSLDNLSLLALTLCVGFVVDDAIVMLENIVRHIEQGETPFDAAL
                     KGAREIGFTIISMTLSLIAVFIPVLFMSGIVGRLLHEFAVTICIAILVSGFVSLTLTP
                     MLCSRYLKHAEPDSHGRLFQIFERFFDALLAAYQDSLKVAMAHPRSMLAGFFATLLLT
                     GLLFTAVPKDFLPSGDSGQILAFTEGAQDVSFASMVKHQRALAEIVAQEPDIRSFMSS
                     VGAGGIRPTANTGTIFMILKPRAERRSSPDEIIQRLRPKLAAVPGIKAYMQNPPVIRI
                     GGQLTAAQYQYTLQDTDLVELYHWAGTLTDRIRQLPGFVDVTNNLNNLSPVLALDIDR
                     DKLASLGLTYGQVEDALQSAFSARQVSTIYGSSNQYQVILEVAPEFQSDPESLPRLYV
                     RSSSGKLIPLDTVARFSRKTQALTVNHQGQLPSVTISFNLLPGVSLGEAVSRIKALEQ
                     EERVPVSLSSSLQGTAKAFEASLQGLGVLLLVAILVVYLVLGILYESFIHPLTILSGL
                     PSAGLGALLTLLIFGVDLSLYAFVGVIMLIGIVKKNAIMMIDFALDRQRRDGIAAAEA
                     IFQACLIRFRPIMMTTMAALVGTLPIALGLGAGAEVRRPLGLAVVGGLLLSQFLTLYL
                     TPVVYLYLDRFSRDPATAQATATVLDSQTPPTDSLPESVCR"
BASE COUNT          702 a         1034 c         1135 g          642 t
ORIGIN      
        1 gggcaatatt atcccaacgt tcgctactgt cccgaaggct ggcaactggt tccggcgcgc
       61 tagccgcgcg agcagcggca ggcggcttgc ggattgtccg ctgcatcgta tgtagtggac
      121 attctggaga cctccggggt atgctcactg ctgacggcaa ggttggggct tgccgtcagt
      181 atcctcgagt agttgtgcag gtcgttgcat ctggcgtggc aggaacgatt ctgcgtggac
      241 aatggcacga tctctttcaa taccgatcag cggagcgtag cttggaaagg cagtggttcc
      301 tggagtccgt cgcggcggat ggctcgcata tgacctacaa catcacccat ctgccgttct
      361 gtatcggccg cgatgccgac aacgacctgg tggtggcctc gcctgacctg agccgacacc
      421 atgtggcgct gacacgcgat atcagcggtc gtattcgact gaccgatctg aacagcacca
      481 acggcagttt cgtcaatcga cagcgcatcg aaggatcctg cctgctcgac gagaacgaca
      541 tcgtgcactt cggcaccact gaattccgga tgcgggtgaa cctgggcggt cacctgcgca
      601 tcgacgacat gcggaccatg ctgatgcccc agggtctgga actgtcggcg cggtttgtgc
      661 gacacgaagc cgagtttctg gcattgctcg aaggacacgg cgtttccggc gccgcgcagc
      721 cgatcgtcga tgcgacgacc aaacgcattg tcgcctacga attgctgggc cgcgccaacc
      781 accccttgct gccgatatcg ccgatccgtc tgttcgaact ggcgagcgcg ctggatcgcg
      841 aaattgacct cagcgtcgct ttccgcaagt tcggtattgc ggcgattgcc ccacgtttgc
      901 agggtttccc gctgtttgcc aatacgcatc cgaaggaaac cttcagcgag gagtttttcg
      961 actcgctggt gagtctgcgc gagcagcggc cggagctcga tctggtgatc gagatccatg
     1021 aaaccgcggt gaccgaaagc gcgcgtctgg tcaaactcgc cgggcgcttc cgggacatcg
     1081 gcgtccgctt cgcctacgac gatttcggtg ccggccaggc ccggctgagc gaactcggcg
     1141 atgcgccggc ccatttcgtc aagttcgaca tgggcctggt gcacgacatc catctggccg
     1201 gcgaacgcaa gcgccgcgtc gtcggcgatc tcgtaaagct cgttctcgat ctgggttcgg
     1261 tgccactcgc cgagggcata gagcaggaag ctgaggcgca gatctgccgg gagatgggtt
     1321 tccagctcat tcagggcttt cttaccggca gaccgattcc ggtagagaat ctgtaggcgg
     1381 cgtttgactg tccaggacgg tcgctgttgc ctgtgccgtc gccgggtctc tggagaagcg
     1441 gtcgaggtag aggtacacca ccggcgtcag gtacagcgtg aggaactgcg agagcaggag
     1501 tccgccgacc accgccagcc cgagaggacg ccggacttcg gcacccgccc cgagtcccag
     1561 ggcgatcggc agggttccca ccagcgctgc catggtggtc atcatgatcg gccggaagcg
     1621 gatcaggcag gcctggaaaa tcgcctcggc ggcggcaatg ccatcgcgcc gttggcgatc
     1681 cagggcgaag tcgatcatca tgatggcatt cttcttgacg atcccgatca gcatgatgac
     1741 cccgacaaag gcgtagaggc tgagatcgac accaaagatc aacagcgtca acagcgcgcc
     1801 gaggccggcc gagggtagtc cggaaaggat ggtcagaggg tggatgaaac tctcgtagag
     1861 gatgccgagc accaggtaga ccaccagaat ggccaccagc agcaggacgc ccagaccctg
     1921 cagcgaggcc tcgaacgctt ttgccgtgcc ctgcaggctg ctgctgagtg aaaccggcac
     1981 acgctcttcc tgttccagcg ccttgatcct gctcaccgcc tcaccaaggg acacgcccgg
     2041 caacaggttg aatgaaatgg tcaccgaggg caattggccc tggtggttca ccgtcagcgc
     2101 ctgcgtcttg cggctgaagc gcgccacggt gtcgagcggg atgagcttgc cgctgctgct
     2161 gcgtacgtag aggcggggca gcgattccgg gtcggactgg aattccggag ccacttcgag
     2221 gattacctgg tactggttgc tcgaaccgta gatggtggag acctgccgcg cactgaaggc
     2281 gctctgcaga gcatcttcca cttgcccata ggtcaggccc aggctggcca gtttgtcgcg
     2341 gtcgatatcc agcgccagca ccgggctgag gttgttgaga ttgttggtga catcgacgaa
     2401 gcccggcaac tgacggattc ggtcggtcaa tgtgccggcc cagtggtaaa gctcgacgag
     2461 gtcggtgtcc tgcagggtgt actggtattg cgcggcggtc agttgtccgc cgatgcggat
     2521 caccggggga ttctgcatgt acgccttgat acccggcacg gccgccagct tcgggcgcaa
     2581 acgctgaatg atttcgtccg gggacgagcg acgttccgcg cgcggcttga ggatcatgaa
     2641 aatggtgccg gtattggctg tcggacgaat gccaccggct ccgacggagg acatgaacga
     2701 gcggatgtcg ggctcctggg caacaatctc ggcgagggca cgttgatgtt tgaccatcga
     2761 ggcaaaagac acgtcctggg cgccttccgt aaaggcaagg atctgccccg agtcgccact
     2821 cggcaggaag tccttgggca ccgcagtgaa cagcaggccg gtcagcagca gcgtggcgaa
     2881 aaaccccgcg agcatgctcc gggggtgcgc catcgctacc ttgagactgt cctggtaagc
     2941 cgccagcaag gcgtcgaaga agcgctcgaa aatctggaac agacggccgt gcgaatcggg
     3001 ttccgcgtgc ttgaggtagc ggctgcagag catcggcgtg agggtcagcg acacgaagcc
     3061 ggagaccaga atcgcgatgc agatggtgac cgcgaattcg tgcagcaggc gcccgacaat
     3121 gccgctcatg aacagtaccg ggatgaacac ggcaatcagc gagagggtca tcgaaatgat
     3181 cgtgaagccg atctcccggg cgcccttgag cgccgcgtcg aagggcgttt cgccctgttc
     3241 gatgtggcgg acgatattct ccagcatcac gatcgcatcg tcgacgacaa agccgacaca
     3301 cagcgtcagc gccagcagcg agagattgtc caggctgtaa cccagcagcg acatcgcgcc
     3361 aaaagtgccg acgaccgaaa tcggcagggc cagcgccggg atgaccgtcg ccgagaggtt
     3421 tctcaggaac agcaggatca ccaggatgac caggaaaccg gccagaatca gcgtgaactg
     3481 gacgtcgtca atggcttcgc ggatcgacac gct
//
LOCUS       DAOSIT010000211         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23398_length_3507_cov_7.925840, whole genome shotgun sequence.
ACCESSION   DAOSIT010000211 DAOSIT010000000
VERSION     DAOSIT010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23398_length_3507_cov_7.925840"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..105
                     /locus_tag="PLL14_06220"
     CDS             22..105
                     /locus_tag="PLL14_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOG03555.1"
                     /translation="MTIPEPESLALFGIGLVGLFLGRRRRV"
     gene            275..1798
                     /locus_tag="PLL14_06225"
     CDS             275..1798
                     /locus_tag="PLL14_06225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOG03556.1"
                     /translation="MAVLFGVCLLISGVVIRLSVGLAARFGIMDCPGGHKQHAASTPF
                     VGGFGLIAVLIGAALLGDPIFSEIPFLPLAAILVGAVAIFLTGLADDIWHLSFKPRLA
                     IQALVALSMVFLGGVELDSLGELLPGLHINLGWLGIPLTVFATIGLINAVNMIDGVDG
                     LSGSVSMVSLALIALVAYAGNAGGYVLFTVALMGGVGGFLYYNLRYPGNRRARVFLGD
                     NGSMLLGFLFAWLLIALSQGNHAAMTPVTALWLFALPLMDTVGVMLRRIWLGKSPFRP
                     DRHHLHHLFIRAGFRVCDVVVIATVMQIVLGLVGVGGLLLGVPDYLMFGLFLAVFAVY
                     FLLILRPWRLVPGLCRLNRSLGLPSVHIRGIFVGSLRKEQWPEILRLIAAELGTAYAY
                     QFNVYQLGESTLDGRNVYCVVHVPAEGNDFLIGRIRRDAMRIRRRLARQQGLAVRLFV
                     CRDVENELGDELMARDGSRSADRRGVRRTLIYSAEHGQKLVHGDDASFELSVPPACR"
     gene            1805..2245
                     /locus_tag="PLL14_06230"
     CDS             1805..2245
                     /locus_tag="PLL14_06230"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOG03557.1"
                     /translation="MFKRILTVCTGNICRSPAAEFLLRQRIEKAGKRVEARSAGIGAL
                     VNHPAEEATCAMMNARGVDLSPHRASQLTRERLRWAELVLVMENHHRDAVLAMDPTAR
                     GKTFLLGHWTKTEIPDPYRRGDAAHAEALQLIEAALEPWVKKLC"
     gene            2573..>3507
                     /locus_tag="PLL14_06235"
     CDS             2573..>3507
                     /locus_tag="PLL14_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG03558.1"
                     /translation="MRNNYEEFSMNMSSALPDGVRSLLACLAASILAGCTIVPGNQGY
                     SNRDESDVRLPVQQGDVLAPANIKIKPITAELIIDLFKSARPPTGDGTSAAKVASKSN
                     LRYTGDQPAAVPEYRLGPGDIVSIIVWDHPELTIPAGSFRSAEQAGTVIAEDGTIFFP
                     YVGVVKAQGKTTSELRTILSSKLAKFIERVQLDVRMVAFRSQQVYVVGEVAKPGIQQV
                     TDIPMTVLDAVNRAGGFTPEADYSRVLLTRRGTTYLVDIQAMYDYGVREQNVMLEQGD
                     IVNVSDRSYNKIFVLGEVTKPGSLVMNKKRSTLAEA"
BASE COUNT          585 a         1081 c         1121 g          720 t
ORIGIN      
        1 tcctacaccg gcaactacga cgtgaccatt cccgaacccg aatccctggc tctgtttggc
       61 atcggcctcg tcggcctgtt cctcggccgt cgtcgtcgcg tctgacaacg tctgagacct
      121 cctcggcgcg agagcgcctg cgggatgtgc aagcggagcc cgaaagggct ctgcttgtgt
      181 agggcccctg aagtggatgg gcggctcggt aggtaaggac gattgatggc gggctggcgc
      241 tctgaggcag cttattgtgg aggtggctgt ggagttggcc gtattatttg gagtttgtct
      301 gctgatctcc ggtgtcgtga tccgattgag tgtcggtctg gccgcgcgtt tcggaatcat
      361 ggactgcccg ggcggacaca aacagcacgc cgccagcacg cccttcgttg gcggtttcgg
      421 gctgatcgcc gtgctcatcg gcgcggccct gctcggcgac ccgattttca gcgagatccc
      481 ttttcttccc ctggccgcga ttctggtcgg ggcggtggcc atcttcctga ccggccttgc
      541 cgacgatatc tggcacctga gtttcaaacc acgtctggcc attcaggccc tggtggcgtt
      601 gtcgatggtt tttctcggcg gcgtcgaact cgactcgctg ggtgaacttc tcccgggctt
      661 gcacatcaat ctcggctggc tcggcatccc cctgacggtc ttcgccacca tcggcctgat
      721 caacgcggtg aacatgatcg acggcgtcga tggtctgtcc gggtcggtga gcatggtcag
      781 tctggcgctg atcgccctgg tggcttatgc cggcaacgcc ggcggctacg tccttttcac
      841 cgttgccctg atgggcggtg tcgggggatt cctgtattac aacctgcgtt atccaggcaa
      901 ccgtcgggcg cgcgtctttc tcggcgacaa cggcagcatg ttgctcggtt ttctgttcgc
      961 gtggttgttg atcgccctgt cgcaggggaa ccatgcggcg atgaccccgg tgaccgcgtt
     1021 gtggctgttc gccttgccgc tgatggatac cgtcggggtc atgttgcgcc gtatctggtt
     1081 gggcaagtcg cctttccgcc ccgatcgcca tcacttgcat cacctcttca ttcgtgccgg
     1141 gtttcgcgtc tgcgatgtgg tggtgatcgc cacggtcatg cagatcgttc tcggtctggt
     1201 cggcgtcggc ggcttgcttc tgggcgtgcc cgattatctg atgttcgggt tgttccttgc
     1261 cgtcttcgcg gtctatttcc tgctgatcct gcgtccctgg cgcctggtgc cgggattgtg
     1321 ccggctcaat cgctcgctgg gactgccctc ggtgcacatc cgcggcatct ttgtcggctc
     1381 gctgcgcaag gagcagtggc cggagatcct gaggctgatt gccgcggaac tgggtaccgc
     1441 ctatgcctac cagttcaatg tctaccaact cggcgagtcg acgctcgacg gccgcaatgt
     1501 ctattgcgtg gtgcacgttc ctgccgaggg caacgatttc ctgatcggca gaatccgccg
     1561 tgatgcaatg cgcatccgaa ggcgtctcgc ccggcagcag ggtttggcgg tgcgcctgtt
     1621 cgtctgtcgc gatgtcgaga acgaactggg cgacgaactc atggcccggg acggcagccg
     1681 gtccgccgat cgtcgtggtg tcaggcgtac gctgatctac tcggcggagc atgggcagaa
     1741 actggtccat ggcgatgacg cgagctttga actcagcgtt ccgccggcgt gcaggtgagt
     1801 tgacatgttc aagaggattc tcacggtctg taccggcaat atctgtcgca gcccggcggc
     1861 cgagttcctg ctgcggcagc gtattgaaaa agccggcaag cgggtggaag cccgctcggc
     1921 aggcatcggc gcattggtca atcatcctgc cgaagaggcc acctgcgcga tgatgaacgc
     1981 acgcggcgtg gacctcagtc cgcaccgcgc cagccagttg acgcgtgagc ggctgcgctg
     2041 ggccgaactc gtgctggtca tggaaaacca tcaccgggac gcggtgctgg cgatggaccc
     2101 gacggcgcgt ggcaagactt tcctgctcgg gcactggaca aagaccgaga ttcccgaccc
     2161 gtatcggcgc ggcgacgccg cccatgccga agccctgcaa ctgatcgagg ccgcgctcga
     2221 accctgggtg aaaaaactct gctgatccag ggcgaatcgc cctttcgccg ggtttcccgg
     2281 ggatgtgaaa taaaacactg gcagtcgcgg cgaccggcgt tatccttcaa agctgattta
     2341 acaaactccc ccagcagcat cagcacccag gcgcgcagac agccgcgcgc agtcaaggaa
     2401 cttgcatggc aatatgtgca agcggtctgc agcgggtacc tgccgtgcct ccggggagag
     2461 cggcagtgcg ggccgatcgg ctgtgcgttg cggagtggct ttgcggcgga ccggcgcgcg
     2521 atgtaaactg cgcggcgccc cttgtcgaga cgagacgtct tcggcaaacc agatgaggaa
     2581 caactatgag gagttcagca tgaacatgtc ttcggctctg cctgacgggg ttcgttccct
     2641 cctggcgtgc cttgccgcga gcatcctggc tggctgcacc atcgttcccg gcaaccaggg
     2701 ctacagcaac cgcgacgagt cggatgtcag gctgccggtg cagcagggcg atgtgctggc
     2761 gccggcgaac atcaagatca agccgatcac cgccgaactg atcatcgacc tgttcaagtc
     2821 cgcgcggccg cccaccggcg acgggaccag cgccgcgaag gtggccagca aaagcaatct
     2881 tcgctacacc ggagaccagc ccgcggcagt gcccgagtac cgcctcggac cgggagacat
     2941 tgtctcgatc atcgtctggg accaccccga actgaccatc ccggccggca gcttccgctc
     3001 cgccgagcag gccggcacgg tgatcgccga agacgggacg atctttttcc cctacgttgg
     3061 agtggtcaag gcgcagggca agacgaccag cgagttgcgc accatcctgt cgagcaagct
     3121 cgccaagttc atcgagagag tgcagctcga cgtccgcatg gtcgctttcc gcagccagca
     3181 ggtgtatgtc gtcggcgaag tggccaagcc ggggattcag caagtcaccg acattccgat
     3241 gaccgtcctc gacgcggtca accgcgctgg tggcttcact cccgaggccg actacagccg
     3301 cgtgttgctc acccgccgcg gcacgacgta tctggtcgat attcaggcga tgtacgacta
     3361 cggggtccgc gagcagaacg tcatgctcga gcagggcgac atcgtcaatg tcagcgatcg
     3421 cagttacaac aagatcttcg tcctcggcga ggtcaccaaa ccgggttcgc tggtgatgaa
     3481 caagaagcgc tcgacgcttg ccgaagc
//
LOCUS       DAOSIT010000212         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23415_length_3505_cov_9.029275, whole genome shotgun sequence.
ACCESSION   DAOSIT010000212 DAOSIT010000000
VERSION     DAOSIT010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23415_length_3505_cov_9.029275"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLL14_06240"
     CDS             complement(<1..658)
                     /locus_tag="PLL14_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOG03559.1"
                     /translation="MTIVAVVGLGYVGLPLAVEFGKQRRTIGFDLSESKVAHYRQYVD
                     PTGEVSSADLKAAIHLQVGTDPAALAEADFLIVAVPTPVDQAHQPDFGPLIGSSEAVG
                     RNLKRGATVVFESTVYPGATEEICIPIIEKYSGMTWKKDFFVGYSPERINPGDKQRTL
                     TKIVKVVSGDTPETLQRVSQVYGSVITAGVYPASSIKVAEAAKVIENTQRDLNIALMN
                     E"
     gene            complement(707..3490)
                     /gene="prsT"
                     /locus_tag="PLL14_06245"
     CDS             complement(707..3490)
                     /gene="prsT"
                     /locus_tag="PLL14_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HOG03560.1"
                     /translation="MNTRNSLHKLRRAGVSALLATLLLVGCGGEKPEAMLASAKEYLV
                     KNDHKAAAIQLKNVLQKEPQLAEARFLLGKALLEGGDPTGAEVELRKARDLKYSADQV
                     TPLLARTMLMLGQPQKVTDELATTELSTPEARADLLTSVGQAYLMQEKDSRAEAAYAA
                     ALVAQPGYAPALLGQARLQASRGDLTGAVALLDSALEKSPKLHDALQLKGDMLATNGD
                     SAGAMAAYRKVLEVKPEYLPAHSAIIRMLLADNKLDEASKQIDSLKETAPNHPQTHYL
                     QAMYAYQKKDYAAARDSVLQHLKFYPESPLGLQLAGLIEYEVKAYPQAEAYLLKALPR
                     TPPLSITRRVLIASYLRDSQPGKALGILEPVLDKIDKDSNMLALAGQVYLQNGQVDKA
                     GPYFAKAAALDPKNAGKRTSLAMVNMLKGDSDVAFRDLEQVASGDTGTRADLALIAAH
                     MQRREYDQALKAIAALEKKQPDNPLTHNLRGTTLLGKRDTAAARKSFEKALALNPAYF
                     PAAANLASLDLADKKPDDARKRFEGVLAKDPKNVQALLAIADLRARAGGTTEEVAGLI
                     GKAIAASPTDPAPRLALIGMYLSKKEVKSALVAAQEAVAVLPERAEILDAAGRAQQAA
                     GDYNQALATYGKLAALKPDSQIPFLRMAEIEVASNNKEAALQSLRKALKVKPDSVEAQ
                     RGIMMLELDAGRIKEALVVAREVQRQRPKEAIGYSLEGDAHASKNAWADAAAAYRAGL
                     KEVPVSELAGKLYSALLGGGNTAEAEKFAETWLKEHPKDARFRLQLGELATARKDYAG
                     AAKQYRLLLEAQPENAAVLNNLAWVAGQMKDPKAIEYAEKANKLAPNQPAVMDTLAVL
                     LLDKGDSARALDLLKKALELAPQASQIRLNYAKALLKAGQKAEARNELDQLAKLGDKF
                     PAQAEVGQLLKGL"
BASE COUNT          589 a         1152 c         1023 g          741 t
ORIGIN      
        1 gttcgttcat cagggcgatg ttcaggtcgc gctgggtgtt ctcgatgacc ttggccgcct
       61 cggccacctt gatgctggaa gccggataaa cgccggccgt gatcacactg ccatagacct
      121 ggctgacccg ttgcagcgtt tccggagtat ctccggaaac caccttgacg atcttcgtca
      181 gcgtccgctg cttgtcccca ggattgatcc gctccggaga gtaaccgacg aagaaatcct
      241 tcttccatgt catgcccgaa tacttctcga tgatcggaat acagatctcc tcggtcgcac
      301 ccggatagac cgtcgactcg aaaacgaccg tggcgccacg cttcagattt ctgccaacgg
      361 cttccgaaga tccaatcagc ggtccaaaat caggctgatg cgcctggtcg acgggcgtcg
      421 ggaccgcgac gatcaggaaa tccgcctcag ccagggcagc cggatcagtg ccgacctgca
      481 agtgaatcgc agccttgaga tcggcactgc tgacctcgcc ggtcggatcg acgtattgcc
      541 gatagtgtgc gaccttgctt tccgacagat cgaagccgat ggttcggcgc tgctttccaa
      601 actcgactgc cagaggcaga cccacataac ccagacccac cacagcaaca atagtcatca
      661 actttccatc ctctgagagt catttttttt cagccacaac tgcgtcctac agccccttca
      721 gcagttgccc gacctccgcc tgcgccggga atttgtcacc cagcttcgcc agttgatcca
      781 gctcgttccg ggcctctgcc ttctgcccgg ccttgagcag cgccttggca tagttgagac
      841 ggatctgcga ggcctgcgga gccagctcca gggccttctt gagcagatcc agcgcacgcg
      901 cgctatctcc cttgtcaagg agcaggacgg cgagcgtatc catcaccgcg ggttgattcg
      961 gcgccagctt gttggccttc tcggcatact cgatggcctt cggatccttc atctgtccgg
     1021 caacccacgc cagattgttg agcaccgccg cgttttccgg ctgcgcttcg agcagaaggc
     1081 gatattgctt ggcagcgccg gcgtagtcct tgcgcgctgt cgccagttcc cccagttgaa
     1141 ggcggaaacg ggcatccttc ggatgctcct tgagccaggt ctcggcaaat ttctcggcct
     1201 cggcggtatt gccgccaccc agcagtgccg aatacagctt gcccgccagt tcgctcaccg
     1261 gcacctcctt gagaccggcc cgataggctg cggcggcatc cgcccaggcg ttcttggagg
     1321 cgtgagcgtc accctccagg ctgtagccga tggcttcctt cggtctctgt ctttgcacct
     1381 cgcgggccac caccagcgct tccttgatgc gaccggcgtc gagttcgagc atcatgatgc
     1441 cgcgctgagc ctcgaccgaa tccggcttga ccttcagtgc cttgcgcaga ctctgcagcg
     1501 ccgcttcctt gttgttcgac gccacctcga tctcggccat gcgcaggaag ggtatctggg
     1561 agtcgggctt gagtgcggcc agcttgccat aggtggcgag cgcctgattg tagtcgcctg
     1621 ccgcctgctg ggctcgtcct gcggcatcga gaatctccgc ccgctccggc agcacggcga
     1681 ctgcctcctg ggccgcgacg agcgcgctct tgacctcctt cttgctcaga tacatgccga
     1741 tcaaggccag acgtggtgcc ggatcagtcg gacttgcggc aatcgccttg ccaatcagcc
     1801 cggccacctc ctcggtcgtg ccacccgccc tggcacgcag gtcggcaatc gcaaggagag
     1861 cctgcacgtt cttcggatcc ttggcgagaa ccccctcgaa gcgcttcctg gcatcgtcgg
     1921 gcttcttgtc ggcaagatcc aggctggcca gattggctgc ggccgggaaa taggccgggt
     1981 tcaaagccag agccttttca aaactcttcc gcgccgccgc cgtatcccgc ttgccgagca
     2041 gcgttgtgcc ccgcaaatta tgcgtcagag gattatccgg ctgttttttc tcgagcgcgg
     2101 cgatggcctt caatgcctga tcgtactcgc gtctttgcat gtgcgcggcg atcagcgcca
     2161 gatcggcgcg ggtcccggta tcacccgacg ctacctgttc caggtcgcga aacgccacat
     2221 cgctgtcacc tttcagcatg ttcaccatcg ccagcgaggt ccgcttgccg gcgttcttgg
     2281 ggtcgagtgc ggcggccttc gcaaaatacg ggccggcttt gtcgacctgg ccattctgca
     2341 gatacacctg accggcaagc gccaacatgt tcgagtcctt gtcgatcttg tcgaggaccg
     2401 gctccaggat tcccagcgcc ttgcccggct gcgagtcgcg aaggtaactg gcaatcagca
     2461 cgcggcgcgt gatgctcagg ggcggcgtcc ggggaagcgc cttcagcagg tacgcctcgg
     2521 cctggggata cgccttcacc tcgtactcga tgagcccggc caactgcaga ccgagagggc
     2581 tctccggata aaacttgagg tgctgcagga cggagtcccg ggccgcagcg taatccttct
     2641 tctgatacgc atacatcgcc tgcaggtagt gtgtttgcgg atggttgggg gcagtctcct
     2701 tcaacgagtc gatctgcttg ctggcctcgt cgagtttgtt gtccgccagc aacatcctga
     2761 tgattgccga gtgtgccggc aggtattccg gcttgacttc gaggaccttg cgataggcag
     2821 ccatcgcccc cgccgaatca ccgttggtag ccagcatgtc ccccttgagc tgcagggcat
     2881 catgaagttt cggcgacttt tccaaagccg agtcaagcag cgccacggcg cccgtcaggt
     2941 cgccccggct ggcctgcagt cgggcctggc ccagcagtgc gggcgcgtag ccgggttgcg
     3001 cgaccagcgc cgccgcgtaa gcggcctccg cccggctgtc cttttcctgc atcaggtagg
     3061 cctgcccgac cgaagtcagc aagtcagccc tggcttccgg cgtactgagt tcggtcgtgg
     3121 ccagttcatc ggtcaccttc tgcggttgac cgagcatgag catggtccgc gccagcagcg
     3181 gggtcacctg gtcggccgaa tacttcaggt cgcgcgcctt gcgcagctcg acctcggcgc
     3241 cggtcgggtc accgccttcc agcagagcct tgccgagcag gaaacgcgcc tcggccagtt
     3301 gcggttcctt ctgcaagacg ttcttcaact ggatggctgc cgccttgtgg tcgttcttga
     3361 ccagatattc cttcgccgag gcgagcatgg cttccggttt ttctccaccg caaccgacca
     3421 ggagaagggt agccagcaat gccgaaacgc ctgccctgcg cagtttgtgc agcgagttgc
     3481 gagtattcat aagggtcctc acgga
//
LOCUS       DAOSIT010000213         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23482_length_3495_cov_9.855814, whole genome shotgun sequence.
ACCESSION   DAOSIT010000213 DAOSIT010000000
VERSION     DAOSIT010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23482_length_3495_cov_9.855814"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="tsaD"
                     /locus_tag="PLL14_06250"
     CDS             <1..903
                     /gene="tsaD"
                     /locus_tag="PLL14_06250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG03561.1"
                     /translation="GGVVPELASRDHIRRVVPLLHKVLADSGRSLAELDAVAYTQGPG
                     LAGALLVGAAFAEALATALGVPTVPVHHLEGHLLSPLLSSRPPSFPFVALLVSGGHSQ
                     LMRVSGVGEYELMGETLDDAAGEAFDKTAKLLGLGYPGGPALAALADRGRPGQVKLPR
                     PMLQSGNLDFSFSGLKTAVLTKVRERVTLSDQQRADIARGFQDAIVDVLVSKALRAVK
                     GSGLKQLVVAGGVGANRELRRQLDAQAARARIAVFYPEFEFCTDNGAMIALAGALRLQ
                     AGGAASSKPAGAFAVRPRWPLTTS"
     gene            complement(999..1619)
                     /gene="plsY"
                     /locus_tag="PLL14_06255"
     CDS             complement(999..1619)
                     /gene="plsY"
                     /locus_tag="PLL14_06255"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOG03562.1"
                     /translation="MPPSLITLLVVLAAYLLGSISFALVASRLFGLADPRTYGSKNPG
                     ATNVLRSGHKGAAIFTLFGDAAKGWLAVFLVSRYSSEYAWGSYAVGWVALAVFFGHLY
                     PVFLRFKGGKGVATAAGVLLAIHPWLGLATLGTWILIAFTLRYSSLAALVAAVAAPAY
                     ALLIWGGDSRVLVVGIIAMALVGKHWQNLQRLMAGSEPKIGAGKSR"
     gene            1731..2087
                     /locus_tag="PLL14_06260"
     CDS             1731..2087
                     /locus_tag="PLL14_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOG03563.1"
                     /translation="MDIIFINDLRASTLIGIYPREKAMPQTIEISLQIGTSTASAGAS
                     DDIRDTIDYAMVVERLHAELAARHFNLLERLAEYVATLLLEDFGATWVRVSIAKLGML
                     RGVQRVGVVIERGAAA"
     gene            complement(2098..2751)
                     /locus_tag="PLL14_06265"
     CDS             complement(2098..2751)
                     /locus_tag="PLL14_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOG03564.1"
                     /translation="MKRFLATGLLLGAALLPTLQGCLPMVAAGATGGALATMDRRSLG
                     TQTDDETIEWKTSARVNEKFGETSHTNFTSYNRKVLITGEVASETIKAEIERIVSDVP
                     QVQGVYNELAIGPVTSFSTRSNDSYITTRVKSRFVDSGKFNAIHVKVVTEAGIVYLLG
                     LVTQREADSAIQVARTTSDVKKVVTLLEVVPDAKARELDVHLPTTSKQPPKPTAAGG"
     gene            complement(2748..3341)
                     /locus_tag="PLL14_06270"
     CDS             complement(2748..3341)
                     /locus_tag="PLL14_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HOG03565.1"
                     /translation="MDLTDRISQHFKESAQTKLDAVELLAAPIAEGIEAMVGSLLNNG
                     KILACGNGGSAADAQHLAAELVGRFEVERHELAAIALSTDTSVISAIANDYGYKAVFA
                     KQVRALGQPGDILVAISTSGNSPNVIEAINAAHENDLRVIALTGKGGGAIGEILRDSD
                     VHICVPAERTARIQETHLLIIHCLCDGIDCLLMGVEE"
BASE COUNT          621 a         1077 c         1127 g          670 t
ORIGIN      
        1 ggcggggtgg tgccggaact cgcatcgcgg gatcacatcc gtcgcgtcgt gccgctgctg
       61 cacaaagtgc tggccgatag cggacgttcc ctggccgaac ttgacgccgt ggcctatacg
      121 caggggcctg gcctggctgg cgccttgctg gttggggcgg cgtttgccga agcgctggcc
      181 acggccctgg gcgtaccgac cgtaccggtg catcacctcg aaggtcacct gctgtctcct
      241 ttgctgtcaa gccggccgcc atcctttccc ttcgtcgccc tgctcgtttc gggtggccat
      301 agtcaactga tgcgcgtgag cggtgtcggc gaatacgagt tgatgggcga aacgctcgat
      361 gacgcggccg gcgaggcttt tgacaagacg gccaaattgc tcggtctcgg ctatccgggt
      421 ggcccggcgc tggctgccct ggccgaccgc ggtcggccag ggcaggtcaa actgccgcgc
      481 ccgatgctgc aatccgggaa tctcgatttc agcttcagtg gtctgaagac ggccgttctg
      541 accaaggtac gcgaacgtgt cacgctcagc gatcagcagc gggcggacat tgcgcgcgga
      601 ttccaggatg cgattgtcga cgtgctggtc agcaaggcac tgcgtgcggt gaaaggcagc
      661 ggtctgaaac aactggtcgt cgccggtggc gtcggtgcca accgcgaact gcggcggcag
      721 cttgatgcac aggctgccag agccagaatc gccgtttttt accccgagtt cgaattctgc
      781 accgacaacg gcgcgatgat cgctttggca ggggccttgc gtctccaggc cgggggggct
      841 gcgtccagca agccggccgg cgccttcgcc gtgcggccgc gctggccgct gaccacatcc
      901 tgaggcagcg cctgctgccg gcggcaagtt gttctactgg cggaagaccg gtttggagca
      961 gtcaggggtt tctgcccgtc gcaacgcgtg aaacgcggtc agcgggattt cccggcaccg
     1021 attttcggct cgctgccggc catcaggcgt tgcaggttct gccagtgctt gccgaccagc
     1081 gccatggcga tgatgccgac gaccagtacc cggctgtcac caccccatat caggagcgcg
     1141 taggccggtg cagcgactgc cgcgaccagt gccgccagcg aagaatagcg tagcgtgaaa
     1201 gcgatgagta tccatgtacc cagggtggcc aagccgagcc aggggtggat agccaacagc
     1261 acgccagcgg ccgtagccac tcctttgccg cccttgaagc ggagaaagac ggggtagaga
     1321 tggccgaaaa aaacggccag tgccacccat ccgaccgcgt agctacccca agcgtactct
     1381 gacgagtagc gactgacgag gaacaccgcc agccagccct tggccgcatc gccaaacagg
     1441 gtgaatatcg cagcgccctt gtggccactc cgcaacacat tggtggcgcc gggattcttc
     1501 gagccataag tccgtggatc ggcgaggccg aaaagtcggc tggcgaccaa ggcgaaggag
     1561 atggagccca gcagataagc ggcaagcacc accagcagtg tgatcaggga cggcggcatc
     1621 gggtgtgatc gagtagtcgg tagtgctctg ggttaaaatg cgcgctctgt agtcgcgcta
     1681 cgaaccgggc gccctaccca caattctaca ccgacctgaa gccctcccgt atggacatca
     1741 tcttcatcaa cgacctgcgc gcctcgaccc tgatcggcat ttacccgcgc gagaaggcga
     1801 tgccccagac catagaaatc tcgttgcaaa tcggcacctc gacggccagt gccggtgcca
     1861 gtgacgacat ccgcgacacc atcgattacg ccatggtggt cgagcgcctg catgccgaac
     1921 tcgctgcacg ccacttcaat ctccttgaaa ggctggctga gtacgttgcc acgctcctgc
     1981 tcgaagactt tggtgcgacc tgggtacgcg tctcgattgc caaactcggc atgctgcgcg
     2041 gcgtgcagcg cgtcggcgtg gtcatcgagc gcggcgcagc ggcctgaggt gccctcgtca
     2101 gccgcctgcg gcagtgggct ttggcggctg tttgctggtt gtcggcaggt gcacgtcgag
     2161 ttccctggcc ttggcgtcgg ggacgacttc cagcagcgtc accaccttct tcacgtcgct
     2221 cgtggttctc gctacctgga tcgcggaatc ggcctcgcgc tgggtgacaa ggccaagcag
     2281 atagacgatg ccagcctcgg tcaccacctt gacgtgtatc gcgttgaact tgccggagtc
     2341 aacaaagcgg ctcttgaccc tggtggtgat atacgaatcg ttgctgcgcg tcgagaacga
     2401 agtgaccggg ccgatggcga gttcgttgta gacgccttgc acctgcggga cgtctgagac
     2461 gatacgttcg atttcggcct tgatggtctc ggaagcaacc tcgccggtga tcaggacctt
     2521 gcggttgtaa ctggtgaaat tggtgtgcga ggtctcgccg aatttctcgt tgacgcgggc
     2581 gctggtcttc cattcgatgg tttcgtcatc ggtttgcgtg cccagggaac ggcgatccat
     2641 ggtcgccagt gcgccgccgg tggcgccggc agcgaccatc ggcagacaac cctggagcgt
     2701 cggcaggagg gctgcgccga gcagcaggcc ggtagccaga aaccgtttca ttcttcaact
     2761 cccataagca gacaatcgat cccatcgcaa aggcagtgga tgatcagcag atgcgtttcc
     2821 tgaattcgcg ccgtacgctc ggccggcacg cagatgtgga cgtcgctgtc gcgcagaatc
     2881 tcgccgatgg cgccgccgcc cttgccggtg agcgcaatga cgcgcaaatc gttctcgtgg
     2941 gcggcattga tcgcttcgat gacgttgggt gaattgcccg aggtcgagat cgccacgagg
     3001 atgtcgccgg gttgccccag ggcgcgcacc tgcttggcga acacggcttt atatccatag
     3061 tcgttggcga tcgccgaaat caccgaggtg tcggtactca gcgcgattgc cgccagttcg
     3121 tggcgctcca cctcgaagcg tccgaccagt tcggctgcca ggtgctgcgc gtcggccgcc
     3181 gagccgccgt tgccgcaggc aaggatcttg ccgttgttca gcagactccc gaccatcgcc
     3241 tcaatccctt cggcaatcgg cgcggccagc aactcgaccg cgtcgagttt ggtttgtgcg
     3301 ctctccttga agtgctgact gatacggtcg gtcaagtcca tgggcggtga ttctgttctg
     3361 cggaaaggag tgccgacgat tctaacatca cagcgagagg aagacctcga actcgattcg
     3421 tcgccaggga ttcgggtccg cccgcagctt cgaaacgcgt gcctcgagtt tctcgccggc
     3481 gtcgagggcg gtggc
//
LOCUS       DAOSIT010000214         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23517_length_3491_cov_8.091676, whole genome shotgun sequence.
ACCESSION   DAOSIT010000214 DAOSIT010000000
VERSION     DAOSIT010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23517_length_3491_cov_8.091676"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLL14_06275"
     CDS             complement(<1..419)
                     /locus_tag="PLL14_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG03566.1"
                     /translation="MRIWTLAVPFSVLLAAGCVSQQTYDQQVQQTQKAQTLERQYQAL
                     NRQLQSEVAADQVQIKQLQDRLVVTFVDEILFASGSAEMHAKGRNVINQAVPTLVGLT
                     GHWIDVQGYTDSEPIGGALRARYPSNWELSAARAADVV"
     gene            complement(490..1149)
                     /locus_tag="PLL14_06280"
     CDS             complement(490..1149)
                     /locus_tag="PLL14_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008049868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOG03567.1"
                     /translation="MKPAIALFISLFTTLVAIINPLEAIPVYLGLMDGKSDDEQRGVA
                     RRACFYAMLLCFFFLFFGTLLLRLFEVPLSMVRVVGGVILTRVGFELFAPTPSGGLIP
                     RADTQTDVAFIPLAMPIMFGPGAIATVISMASTIKQSDTELIHFVAACAAIVACLGTT
                     YLSLIYAKPILRKIGNQGIDAATRIVGFFVAAMGMGLIFQGAVEFLAPYGRVAGVAVG
                     T"
     gene            1347..>1823
                     /locus_tag="PLL14_06285"
     CDS             1347..>1823
                     /locus_tag="PLL14_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOG03568.1"
                     /translation="MVFSFLSFVHLTLAVAVSLRIFSRRSSRGSALAWLLLVLLVPAV
                     GALAYLLIGERRLGKTWMQRAIALQPQILRWAQRIPATTVAAPGFLDRGAESISRLAT
                     GSIGLPLLGGHRLQLLADSASIMRALIADIDAARTSVHMEFYIWSAGGFVDELVAAL"
     assembly_gap    1824..1923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1924..2637
                     /locus_tag="PLL14_06290"
     CDS             <1924..2637
                     /locus_tag="PLL14_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOG03569.1"
                     /translation="RFFKQDAGVGEWVDAMVRIEGPAAWVLEAVSLSLTALQTGADFA
                     PPPPPVLPRTGDSHVQIFPSGPQGATRHIEALLLTAVYAARHEIVLTTPYFIPSEPLL
                     TALRSAAIRGVRVILIVPAKVDSLLVRYASNAYIEDLLSVGITILHFGDGLLHTKSMV
                     IDEQITIFGTVNLDLRSFELNFEVSLIAYDQAFAVQTRRLQSEYEARSLPLQLAQWHA
                     RPQWRRLLENAVQTMSPLL"
     gene            2981..>3491
                     /locus_tag="PLL14_06295"
     CDS             2981..>3491
                     /locus_tag="PLL14_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOG03570.1"
                     /translation="MNTEVPERKSSGFKLFQGLLPIQKAAALRDALAGFTLAAMNVPQ
                     ALGYTKIAGTPVVSGLYTLLLPLLAFAALGSSRYLVVAADSATAAILATGLAGLAAVD
                     SAHYLALASLVALLTAGFLLLARLFKLGFLSDFLSRTVLVGFLTGVGFQVSIAVLDEM
                     LRVPVSSHQP"
BASE COUNT          634 a         1128 c          997 g          632 t
ORIGIN      
        1 accacatccg cggcacgcgc cgccgaaagc tcccagttgc tcggataacg ggccctcaac
       61 gccccgccaa tcggctcgct gtcggtataa ccctgcacgt cgatccagtg gccggtcagg
      121 ccaaccaggg tcggaaccgc ctggttgatc acattccgac ccttggcgtg catctcggca
      181 ctgcccgaag caaacaggat ctcatcgacg aaggtcacca ccaatcgatc ctggagttgt
      241 ttgatctgca cctggtccgc cgcaacttcc gactgcagtt gcctgttcag cgcctggtat
      301 tgccgctcga gcgtctgcgc cttctgcgtc tgttgcacct gctgatcgta ggtctgctgc
      361 gatacgcagc ctgccgccag caacacactg aacggcaccg ccaatgtcca aattctcatc
      421 tctgcgaact ccttcatcaa ggtaaatcca gcgctctcgg gccgccgggc ggcggcaatg
      481 atagcgcacc taggtcccga cagcgactcc ggccacacgg ccataaggcg ccaggaattc
      541 caccgcgccc tgaaatatca ggcccatacc catcgccgcg acgaaaaatc cgacgatccg
      601 cgtagcggca tcgatgccct gattgccgat cttgcgcaga atgggcttgg cgtagatcag
      661 ggatagatag gtggtcccga ggcacgccac gatcgccgcg caggcggcga cgaaatggat
      721 caactcggtg tccgactgct tgatggtcga cgccatgctg atcactgttg caatcgctcc
      781 gggaccgaac atgatcggca tggcgagcgg aatgaaggca acgtcggtct gcgtgtccgc
      841 ccgtggaatc aaaccacccg acggcgtagg cgcaaacaat tcgaaaccga cgcgggtcag
      901 aatcacgcca ccaaccaccc gcaccatgct cagcggcacc tcgaacaagc gcagcagcaa
      961 ggtgccgaaa aacagaaaga aaaaacacag caacatcgcg tagaaacagg ccctgcgggc
     1021 gacaccgcgc tgctcgtcgt cggacttgcc gtccatcaat ccgagataga ccggaatcgc
     1081 ttccagcggg ttgatgatcg caaccagcgt ggtaaacagg ctgatgaaaa gggcaatggc
     1141 aggtttcatg gggcagcggt cctgttcaag acatgggatc gctcacccgg atcaacgagc
     1201 agaatcctca tgaggggcaa gcgacgagcg ccgtgaggct atcacaaaca tttgtcacgg
     1261 cgacgattcc tcgacgctaa ccgcggccgg atccgcaagc gggcgctcgt tgaccgagtc
     1321 ggcgcagcag ccgcatactc cggggcatgg ttttcagttt cctgtccttc gttcacctga
     1381 ccctggccgt ggcggtttcc ctacgcatct tttcacgccg gagttcacgt ggatcagccc
     1441 tggcatggct gctgctcgtt cttctcgtac ccgccgtcgg cgccctggct tatctcctga
     1501 tcggcgagcg ccgtctcggg aagacctgga tgcaaagagc gattgccctg caaccacaaa
     1561 ttctccgctg ggcacagcgt atcccggcca cgaccgtggc tgcgccaggt tttctggacc
     1621 ggggagccga gagcatcagc cgcctcgcca cgggttcgat cggtctgcca ctgctgggtg
     1681 gccatcgcct gcagctgctc gccgacagtg cgtccatcat gcgcgcgctg attgccgaca
     1741 tcgacgccgc ccgcacgtca gtgcacatgg agttctatat ctggagtgcc ggcggtttcg
     1801 tcgacgaact ggtggcggcg ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnncgcttct tcaagcagga tgccggagtt ggcgagtggg tggatgcgat ggtccgcatc
     1981 gaagggccgg cggcctgggt actcgaagcc gtctcgctgt ccctgacggc cctgcagacc
     2041 ggagccgact ttgccccccc gccaccgccc gtgctgccgc gaacgggtga cagccacgtc
     2101 cagatcttcc cttccggccc gcagggggcc acccggcata tcgaggcact gctgctgact
     2161 gcagtgtacg cggcccgcca cgagatcgtc ctgaccaccc cctacttcat tcccagcgag
     2221 cccttgctga ccgccctgcg ttcagccgcg atacgcggcg tccgcgtcat cctgatcgtg
     2281 ccggcgaagg tcgactcgct gctcgtccgc tacgccagca acgcctatat cgaggacctg
     2341 ctgagcgtcg gtatcaccat cctgcacttc ggcgacgggc tgttgcacac caagagcatg
     2401 gtcatcgatg agcagatcac gatcttcgga acggtcaatc tcgacctgcg cagcttcgaa
     2461 ctcaatttcg aggtctcgct gatcgcttac gaccaggcct tcgcggtcca gacccgccgg
     2521 ctgcaaagcg agtacgaagc aagatcgcta ccgctgcaac tcgcacagtg gcacgcccgg
     2581 ccacagtggc ggcgactgct ggagaacgcc gtacagacca tgagcccgtt gctctgacat
     2641 cggcagtgtg ctgcgacaga gaaccgttag catcacggac catgcacccg cgccgacttc
     2701 ttccgatctt tctgctgccg gccggcctca acgcgcgcag cacagccgtc gacccggcgg
     2761 cgcgcgcaac acactgaacg tctcggaaaa tcgccgcgca tcataggcgc cccttggcga
     2821 gcgtgcgcgt cgtggtgccc ggcggccctc ttgacttcgt ttgcccgaac cgcagatact
     2881 ccgctgccag ttcacctgag tgtttgcgcg ttgacgctga cccttgcagg ctggtcgcag
     2941 gcctgaccat gacctcaacc acaccatggc gaggagccgc atgaataccg aagtaccaga
     3001 aaggaagtcg agcggattca aactgtttca gggcctgttg ccgattcaga aagctgcggc
     3061 gctccgcgac gcactggctg gtttcacgct ggcggcgatg aacgtgccgc aggcgctggg
     3121 ttacaccaag atcgccggga caccggtggt cagcggcttg tataccctgc tgctgcccct
     3181 gctggctttc gctgcgttgg gttcgtcacg ctacctggtg gtggcggcag actcggcgac
     3241 ggcggcgatc ctcgcgactg gactggcggg actggccgcc gtcgatagcg cgcattacct
     3301 ggcactggcc agtctggtgg ccctgctgac cgccggattt ctgctgctgg cgcggctgtt
     3361 caagctcggc ttcctgtcgg actttctttc tcgcacggta ttggtcggct tcctcaccgg
     3421 cgttggattc caggtcagca ttgccgtgct cgacgaaatg ctgcgcgtgc cggtaagcag
     3481 ccaccaaccg c
//
LOCUS       DAOSIT010000215         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23538_length_3489_cov_7.049505, whole genome shotgun sequence.
ACCESSION   DAOSIT010000215 DAOSIT010000000
VERSION     DAOSIT010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_3489_cov_7.049505"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLL14_06300"
     CDS             <1..1032
                     /locus_tag="PLL14_06300"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOG03571.1"
                     /translation="YSVLRQLLFTLDPETAHGIGMAGVDWLKAARATCLLARPVPPDP
                     VSVMGINFPNPVGLAAGVDKNGEHIDALAALGFGFIEIGTITPRPQPGNPRPRLFRIP
                     ERQAIINRMGFNNDGVDQLLAHVDAADFPRRGGILGINIGKNFDTPIDKAADDYLFCL
                     ERVYAAASYVTVNVSSPNTRNLRELQRDEALDALLGSLKSQQSRLADRHAKYVPLALK
                     IAPDLDDSQVDAIAALLCRHRMDGVIATNTTLARVGVEGLPNAEQAGGLSGAPLLAMS
                     NAVLNKLSVALAGEVPIIGVGGIISGADAAAKIAAGARLVQLYTGFIYRGPELVAEAA
                     AAIARQRRV"
     gene            1130..1714
                     /gene="rsxA"
                     /locus_tag="PLL14_06305"
     CDS             1130..1714
                     /gene="rsxA"
                     /locus_tag="PLL14_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG03572.1"
                     /translation="MTHYLFIIIGAVLVNNVVLVRILGLCPFMGVSKKLETAFGMGAA
                     TTFVLTVATGASYVIDHYLLMPFGLEYLRTLSFIVTIAAIVQLTEMVIQKTSPLLHQV
                     LGIYLPLITTNCAVLGVPLLNVANKYNFVESLLFGAASAIGFSLVLVLFAGIRERVEG
                     ADVPTCFRGTAIAMVTAGLMSLAFMGFAGLDKYQ"
     gene            1711..2253
                     /gene="rsxB"
                     /locus_tag="PLL14_06310"
     CDS             1711..2253
                     /gene="rsxB"
                     /locus_tag="PLL14_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOG03573.1"
                     /translation="MITTLAIMALGAVVLGAALGYASIRFKVEGDPLVEKIEAILPQT
                     QCGQCGYPGCKPYAAAISAGEAEINLCPPGGIDGVRKLADLLGREVKPLEAEEKPKQV
                     AFIDEQTCIGCTLCIQACPVDAIVGAAKQMHTIVEPLCTGCELCIKPCPVECIIMQPI
                     AENIDNWKWKYPVIEIKRAA"
     gene            2264..>3489
                     /gene="rsxC"
                     /locus_tag="PLL14_06315"
     CDS             2264..>3489
                     /gene="rsxC"
                     /locus_tag="PLL14_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG03574.1"
                     /translation="MLQQLFSFKGGVKPQTRKAPSVQSPIGQAPMPVRLYVPLHQSLG
                     GAPNPLVQAGEQVLKGQVIGAADGWMSAAVHAPTSGTVLAVEQHVAAHPSGLPTLSVV
                     IEPDGREQWIARTPLDHRSLAPERVRELLRDAGVVGLGGAVFPSHAKLTAARTVPVEH
                     LVINGAECEPFMTCDDLLMRERAEEIVRGTAIFRDLLAARRVLIGIEDNKPEAVAAMQ
                     LAVRRLGEDHEVVAVPTRYPAGGAKQLIRVLTGKEVPASKRSTELGVQCFNVATAYTA
                     YRALAHGEPVISRIVTLTGNVEQPRNWEVLLGTPMREVVALGKPKADTSRYLMGGPMM
                     GFEIGDLAAPVVKATNCIIAGSSTLFPPNPPEMPCIRCGACADVCPHELQPFELYWFA
                     RARNFGKTQEYHIFDC"
BASE COUNT          613 a         1099 c         1091 g          686 t
ORIGIN      
        1 tattccgtgc tgcgccagtt gttgtttacc ctagaccccg aaaccgcgca cggtatcggc
       61 atggccggcg tcgactggct caaggcagcg cgagcgacct gccttctggc caggccggtg
      121 ccgccggatc cggtgtcggt catggggatc aatttcccca atccggttgg cctggccgcc
      181 ggtgtggaca agaacggcga acacatcgac gcgctggccg cgcttggttt cggcttcatc
      241 gagatcggca ccatcacccc gcgtccgcag cccgggaatc ccagaccgcg cctgttccgc
      301 attccggaaa ggcaggcaat catcaatcgc atgggtttca acaacgacgg cgttgaccaa
      361 ctgctggccc atgtcgacgc ggccgatttc ccgcgccgcg gcggtatcct gggcatcaat
      421 atcggcaaga acttcgatac cccgatcgac aaggccgccg acgactatct attctgtctc
      481 gaacgcgtct atgccgcggc cagttacgtg acggtcaatg tctccagccc aaacaccagg
      541 aatctgcgtg aattacagcg cgacgaagcg ctcgatgccc ttctcggaag cctgaaaagc
      601 cagcagtccc ggctcgccga tcggcatgcc aagtatgtgc cgctggcatt gaaaattgcc
      661 ccggaccttg acgactcgca agtcgatgcg atcgcagcgc tgctttgccg gcatcgcatg
      721 gacggcgtga tcgccaccaa taccacgctc gcacgcgtcg gcgtcgaagg cttgcctaac
      781 gccgaacagg cgggtggtct gtccggcgca ccgctcctgg cgatgtcaaa tgcggtcctc
      841 aacaaactgt ccgttgccct ggctggcgaa gtgccgatca tcggcgtcgg cggcatcatc
      901 agcggtgcag atgctgcggc gaaaatcgcc gctggtgcgc ggttggtgca actgtacacg
      961 ggcttcatct accgcggccc cgaactggtt gcagaagcgg ctgctgcgat tgccaggcaa
     1021 cgccgtgttt gagttgcgat agcggtacgc ataacgtggt tgtatgcgcg cacctgacaa
     1081 gggataatcg cagcgccctg gagtgcactg cctcctgctc acgcgaacca tgacccacta
     1141 tctgttcatc atcatcggcg ctgtcctggt caataacgtt gtcctggtca ggattctcgg
     1201 cctgtgcccg ttcatgggcg tctccaagaa actggagacg gctttcggca tgggcgcggc
     1261 gacgaccttc gtgctcaccg tcgccaccgg tgccagctac gtcatcgacc actacctgtt
     1321 gatgcccttc gggctggagt acctgcggac cttgtccttt atcgtcacca ttgccgccat
     1381 cgtccagctc accgagatgg tgatccagaa gaccagcccc ctgctgcacc aggtactcgg
     1441 catttacctg ccgctgatca ccaccaactg cgcggtcctc ggggtgccgt tgctgaacgt
     1501 ggccaacaaa tacaacttcg tcgagtctct tctctttggt gccgccagcg cgatcggctt
     1561 ttcgctggtc ctcgtgctct ttgcgggtat ccgcgaacgc gtcgagggcg ccgatgtgcc
     1621 gacctgtttt cgtgggacgg ccattgccat ggtcaccgcc gggctgatga gcctggcttt
     1681 catgggtttt gccgggctgg acaagtatca atgatcacca ccctggcgat catggcgctg
     1741 ggcgccgtcg tgctgggtgc tgcgctcggc tatgcgtcga tcaggttcaa ggtcgaaggc
     1801 gacccgctgg tcgaaaagat cgaagcgatc ctgccgcaga cgcagtgcgg ccagtgcggt
     1861 tatccgggtt gcaaaccgta cgccgcagcc atttccgccg gcgaggccga gatcaacctg
     1921 tgcccgccgg gcggcatcga tggggtgcgc aagctggccg atctgctcgg tcgcgaagtc
     1981 aagccgctgg aggctgagga aaaaccgaag caggtggcgt tcatcgacga acagacctgt
     2041 attggctgca cactgtgtat ccaggcctgc ccggtggatg ccatcgtcgg ggctgccaag
     2101 cagatgcaca ccattgtcga gccactgtgc acgggctgtg aactgtgcat caagccctgt
     2161 ccggtcgagt gtattatcat gcagccgatc gccgaaaaca ttgacaactg gaaatggaaa
     2221 tatccggtga tcgaaatcaa gcgggcggcc tgagttgccc ggaatgttgc agcaactgtt
     2281 cagtttcaag gggggcgtca agccacagac ccgcaaggcg ccttcggtgc agtcgccgat
     2341 cggtcaggcg ccgatgccgg tacgcttgta cgtgccgctc caccagagcc tcggtggcgc
     2401 gccgaatcct ctggtgcagg ccggtgaaca ggtgctcaaa gggcaggtga tcggtgctgc
     2461 cgacggctgg atgtcggcag cggtgcatgc gccgacgtcc ggcaccgtgc tggctgtcga
     2521 gcagcatgtg gccgcgcatc cttccggctt gccgaccttg tcggtggtca tcgagccgga
     2581 tggtcgtgag cagtggattg cccgtacgcc gctcgaccac cgcagtctgg cacccgagcg
     2641 tgtgcgcgag ctgctgcgtg acgcgggcgt cgtgggcctt ggcggggctg tttttccgag
     2701 ccatgccaag ctgaccgcgg cccgcaccgt gcccgtcgaa cacctggtga tcaatggcgc
     2761 cgagtgcgag cctttcatga cctgcgacga cctgctgatg cgcgagcgtg ccgaggagat
     2821 cgtgcgcggc accgccatct tccgtgacct gctggcggcc cgacgagtgc tgatcggtat
     2881 cgaggacaac aagcccgagg ccgtcgccgc catgcagctc gcggtgcggc gccttggcga
     2941 ggaccatgaa gtggttgccg tcccgacacg ctacccggcc ggcggcgcca agcagttgat
     3001 ccgtgtgctt accggcaagg aggttccggc cagcaagcgc tcgaccgaac tcggtgtgca
     3061 gtgtttcaac gtggccactg cctataccgc ctatcgtgcg ctggcccacg gtgagccggt
     3121 gatttcacgc atcgtcaccc tgaccggcaa tgtcgaacag ccgcgcaact gggaagtgtt
     3181 gctcggtacc ccgatgcggg aggtggtcgc gctcggcaag ccgaaagccg acaccagccg
     3241 ctatctgatg ggcgggccga tgatgggctt tgaaatcggt gacctcgcgg cgccggtggt
     3301 caaggctacg aactgcatca tcgcgggttc gtcgaccttg tttccgccga acccgccgga
     3361 gatgccgtgc atccgttgcg gcgcctgcgc cgacgtttgt ccgcatgagt tgcagccttt
     3421 tgaactgtac tggttcgcgc gcgcccggaa cttcgggaag acccaggaat accatatctt
     3481 cgactgcat
//
LOCUS       DAOSIT010000216         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23672_length_3473_cov_10.379169, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000216 DAOSIT010000000
VERSION     DAOSIT010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23672_length_3473_cov_10.379169"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PLL14_06320"
     CDS             <1..1358
                     /locus_tag="PLL14_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOG03575.1"
                     /translation="VDVVRIMRAKNISSVVVCENTLPSGIMTDRDLRNKVVAAGTDPS
                     RLVVRTIMNSPLSVIGEEDLLYEALYRMSRERIHRLAVVDDQGKLSGIITDSDIIRLQ
                     AHSPHQLVLDIERTRDFDELKAVYSRIQSLVLHLSGTGMKTRDVVRMIAHLNDQILLR
                     LIALMRQDRFAELPKRFAFVVLGSEGRGEQTLLTDQDNAIVYGDELGPDEIRQIEDFS
                     QVLIDSLVAIGVPPCSGGIMANNKEWRRSLGKWTDQLDRWIQAPTPNHVLSCGTFVDI
                     RTIYGDPSFEQELKTQLYQHVQRDKLFLMRMVENTLRFVPPIGWFGKIKGEKDGEHSG
                     MLEIKKAGIFALSEGVKALAIQAGKLEGSTHQRLEALVRDKVVNKKMAATIAESFDFL
                     VLMRLRGQVEAVREGRKPDNYIQIERLNMMELGRLQIALKGVGKFQEFIKGHFNLHLL
                     R"
     gene            complement(1458..1733)
                     /locus_tag="PLL14_06325"
     CDS             complement(1458..1733)
                     /locus_tag="PLL14_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOG03576.1"
                     /translation="MTETIVAYLRAHAEQLDVDMAEALNIPIAELRDQVARLAASGDV
                     ICCRVTRFIEGRKIEGVSCRLSCDPPTPARGRKPGVKKEDDSETGLF"
     assembly_gap    1819..1918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1919..2169
                     /locus_tag="PLL14_06330"
     CDS             <1919..2169
                     /locus_tag="PLL14_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemoglobin-like protein"
                     /protein_id="HOG03577.1"
                     /translation="QSSQDKLYMFLSGWLGGPDLYVENIGHPMLRRRHLPFAIGESER
                     DQWVACMVLSMESVGIEDPLREQLMLSFFNTADFLRNQ"
     gene            2302..>3473
                     /locus_tag="PLL14_06335"
     CDS             2302..>3473
                     /locus_tag="PLL14_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG03578.1"
                     /translation="MSRAVRNIAIIAHVDHGKTTLVDKLLVQAGSFSAHQHITERMMD
                     SNELEKERGITILAKNLAVDYAGVHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTKKALALGLKPIVVVNKIDRDGARPDWVVDHTFDLFDKLGANDEQMDF
                     PVIYASAINGYATLDLSKPSPDMRPMFETILRHVSPPHADEIDGPLQLQISALDYSSY
                     VGRIGIGRVQRGRLKPAQQVIVMYGQADANGKFEHGAPKTAKVGQVFGFRGLERVSLD
                     EAVAGDIVLVTGIEDLSIGCTITDTEKPEALPMLRIDEPTLNMTFMVNNSPYAGTEGK
                     FVTSRQIGERLRKELLTNMALRVEETAETDSFLVSGRGELHLTILLETMRREGYELA"
BASE COUNT          694 a         1049 c          986 g          644 t
ORIGIN      
        1 tggtagacgt cgtcaggatc atgcgtgcca agaacatttc cagcgtcgtg gtctgcgaga
       61 acacgctgcc cagcggcatc atgaccgacc gtgacctgcg caacaaggtc gttgctgccg
      121 gcaccgaccc gtcccggctc gtcgtacgga cgatcatgaa cagcccgctg tcggtgatcg
      181 gcgaagagga tctgctgtac gaagcgctct accggatgtc gcgcgagcgg atccatcgcc
      241 tggcggtggt cgatgaccag ggcaaactct cgggcatcat taccgatagc gacatcatcc
      301 gtctgcaggc gcattcgccg catcaactgg tcctcgacat cgagaggact cgcgatttcg
      361 acgagttgaa agccgtgtac tcgcgtatcc agagcctggt gctgcacctc agcggcaccg
      421 gcatgaagac ccgcgacgtg gtgcgcatga ttgcgcacct gaacgatcag attctcttgc
      481 gcctgattgc cctgatgcgc caggatcgct ttgccgagtt gcccaagcgc ttcgcttttg
      541 tcgtgctcgg cagcgaggga cgcggcgagc agacactgct caccgaccag gacaatgcca
      601 ttgtttacgg cgacgaactc ggccccgacg aaatccggca gatcgaagat ttttcgcagg
      661 tcctgattga ctccctggtg gccatcggcg tccccccctg ttccggcggc atcatggcca
      721 acaacaagga gtggcggcgc agcctcggca aatggacgga ccagctcgac cgctggatac
      781 aggccccgac accgaaccac gtgctgagct gcggcacgtt cgtggacatc cgtaccatct
      841 acggcgaccc ctcattcgaa caggaactga agacgcagtt gtaccagcat gtgcagcgag
      901 acaagttgtt cctgatgcgc atggtcgaaa acaccctgcg ctttgtcccc ccgatcggct
      961 ggttcggcaa gatcaagggc gagaaggacg gcgaacactc ggggatgctg gagatcaaga
     1021 aggccggcat ttttgctcta tccgagggcg tcaaggcact ggccattcag gccggcaagc
     1081 tcgaaggcag tacccatcaa cgacttgaag ccctggtcag ggacaaggtg gtgaacaaga
     1141 aaatggccgc gacgatcgcc gagagcttcg acttcctggt cctgatgcgc ctgcgcggac
     1201 aggtggaagc cgtgcgcgag ggccgcaagc cggacaacta catccagatc gagcggctca
     1261 acatgatgga acttggcagg ctgcaaatcg ccttgaaggg tgtcggaaaa ttccaggagt
     1321 tcatcaaggg ccacttcaac ctgcatctgc tcagatagcg acgcagcatt tccggtctgc
     1381 gcctgagcca tcaccacaga gcaatgtcag accggtgctc ggggtgtcat tgtctcgcta
     1441 tcggcaccct cgcccgatca aaacagaccg gtttccgaat catcttcctt tttcacgccc
     1501 ggcttccgcc cacgcgccgg tgtcggtggg tcacatgaca gacggcagct gacaccttcg
     1561 atctttcggc cttcaatgaa gcgggttacc ctgcagcaga tcacgtcccc ggacgcggcg
     1621 agccgggcga cctgatcacg cagctcggcg atcggaatgt tgagcgcctc ggccatgtcg
     1681 acatcgagtt gctccgcatg cgcccgcaga taagcaacaa tcgtttcggt catctgaata
     1741 ccctcttaaa tgcctttgcc aactggaaag gtacagcagt gtgcgccgct gccccgcttc
     1801 tggcaattcc tgcgctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
     1921 cagtcctcac aggacaaact gtacatgttc ctttcgggct ggctcggcgg accggacctc
     1981 tatgtcgaga acatcggtca tccgatgttg cgccgccgtc acctgccctt tgccatcggc
     2041 gaaagcgagc gggaccagtg ggtcgcgtgc atggttctgt ccatggagtc cgtcggtatc
     2101 gaagacccgc tacgcgagca attgatgctc tcctttttca acaccgccga tttcctgcgc
     2161 aaccaataac cctgaatttt tcaggccgtc ccagtggggt tctcgcgacg atgggcgccc
     2221 agcgcaaggc tgttttgcgg tgcaacaggc gcagcctgcc tgtgctagaa tagtgcccct
     2281 tgttcaaagg cttgacacta catgtcccgt gccgtccgca acatcgccat catcgcccac
     2341 gtcgaccatg gcaagaccac cctggtggac aagctactgg ttcaggctgg cagcttctcc
     2401 gctcaccagc acatcaccga gcggatgatg gactcgaacg aactggagaa agagcgaggc
     2461 atcaccatcc tggccaagaa tctcgcggtc gactatgccg gcgtacacat caacattgtc
     2521 gacacgccgg gacacgccga cttcggcgga gaggttgagc gtgtgctgtc gatggtcgac
     2581 ggcgtcctgc tgctggtcga tgcggttgag gggccaatgc ctcagacccg cttcgtcacc
     2641 aagaaagccc tcgccctggg gctgaagccg atcgtggtgg tcaacaagat tgaccgcgac
     2701 ggagcccgac cggattgggt cgttgaccac accttcgacc tcttcgacaa gctcggggca
     2761 aatgacgagc agatggactt cccggtaatt tacgcatccg cgatcaacgg ctacgcgaca
     2821 ctggatttgt ccaagccctc gccggacatg cggccgatgt tcgaaacgat tctccggcac
     2881 gtctcgccgc cgcacgcaga cgagatcgat ggcccgctgc aactgcagat ttcggctctc
     2941 gattactcga gctacgtcgg acgcatcggc attggccgcg tgcagcgcgg gcgcctcaag
     3001 ccagcccagc aggtgatcgt gatgtatggc caggcggacg ccaatggcaa gttcgagcat
     3061 ggtgctccga aaaccgccaa ggtcggccag gtgttcggtt tccgcggact cgaacgggta
     3121 tcgctggacg aggcggtcgc cggcgacatc gtcctggtca ccggcatcga ggacctttcg
     3181 atcggctgca cgatcaccga taccgaaaag ccggaggcgc tgccgatgct gcgcatcgac
     3241 gagccgacgc tgaacatgac cttcatggtc aacaactcgc cgtacgccgg taccgaaggc
     3301 aagttcgtca ccagccgcca gattggcgag cgcctgcgca aggaattgct gaccaatatg
     3361 gcgttgcgcg tcgaagaaac tgccgagacc gacagcttcc tcgtttccgg ccgcggtgaa
     3421 ctgcatctga cgatccttct cgaaaccatg cgccgcgagg gctatgaact ggc
//
LOCUS       DAOSIT010000217         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23752_length_3466_cov_9.328936, whole genome shotgun sequence.
ACCESSION   DAOSIT010000217 DAOSIT010000000
VERSION     DAOSIT010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23752_length_3466_cov_9.328936"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1068
                     /locus_tag="PLL14_06340"
     CDS             418..1068
                     /locus_tag="PLL14_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG03579.1"
                     /translation="MTPDARGRADPGPSPAAASRTLPAAAAGIAAEMAGFQREMLRFA
                     VLQLRDQASAEDAVQEAMLAALQGADRFAERAKLKTWVFSILRNKIVDIIRRRVREPS
                     YQAPETEIDDADFDPLFSANGHWHSDSRPADWGDPEKSFENQRFWAIFDGCLNRLPAA
                     TARVFMMREMLGLETDEICTELSMSSNNCWVVLHRARMGLRMCLDQQWFDAGEQTR"
     gene            1065..1268
                     /locus_tag="PLL14_06345"
     CDS             1065..1268
                     /locus_tag="PLL14_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOG03580.1"
                     /translation="MMNCREAVKLMSEEMDHDLSGGRRWSLRLHLLICLGCRNYRRQL
                     SFLRQACRHEVAVDDTPLAPPPT"
     gene            complement(1320..3236)
                     /locus_tag="PLL14_06350"
     CDS             complement(1320..3236)
                     /locus_tag="PLL14_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HOG03581.1"
                     /translation="MPDDPISNDSTNPHFEQLLVASSTLSARRRRFLQLGFGSAALPF
                     LELPLRVAAGAQLGMRFPPLAASSEDRVRVPEGYHVQMLYAWGDPVSDAPGFRGDAGN
                     SAAEQAEQAGMHHDGMHFFPFVRHGQPSSTHGLLCVNHEYTDEGLLHPDGMADWSHAK
                     MLKSQHAHGVSVIEVELAGSGRDLAWRVLRPSAYARRITARTPMRIDGPAAGAAAMRT
                     RDDRRGDVVFGTLANCAMGVTPWGTYLSCEENFNAFFNGLDQPTAAQQRYGLRARGAG
                     MRWHEHDRRFDLASEPNEANRFGWVVEIDPWNPASVPVKHTALGRFKHEGAMLTLAGD
                     RRVVVYMGDDEAFEYLYKYVSRDRFDAGKAAGGSLLAIGTLHVARFGADGRGHWIALQ
                     HGQNGLTEANGFADQADILIRTRQAADLVGATKMDRPEWLAVHPGSGEVYCSLTNNRQ
                     RVDVDAANPRRGNVFGHIIRWREDGGDAAAGSFGWDLFVLGGDPQHADPEKRGNSRGD
                     AFGSPDGLWFDRDGRLWIATDVSGSAMHRGDYARLGNNQLLVADVVSGEVRRFLTGPR
                     GCEITGPTATPDGRNLFINVQHPGELPGGRSDPTRPLAGSGWPASQFANVTGGRPRSA
                     TIVVRRDDGGIVGA"
BASE COUNT          596 a         1152 c         1139 g          579 t
ORIGIN      
        1 gttcttgatt tccctgggta cgccgatgtg catctctgtc tccttgccag tggttgaggg
       61 ctgctcatct tattgcattt cgttcgcaat ggcggcattt ttgggtcgcc acgcgccagt
      121 tcaacggcat ggcaggcggg gagcggcagg gcacgcgcgg cagctctccg cacacccggc
      181 agccagggta cccgctgctg ttccatgccc cggcaactgc gtatagactg cgaaccctgt
      241 tcccgtcctg ctgttgcgcg ggcactgctc cggcagtatg tccggcgacc tggcggtccc
      301 cttcgcgccg aagcgcagag taaccggcgt cggcacttgc aaggctgaat gccacttttt
      361 ggagagatcg gaatccgtgc cggacttgtg tgaatgctca gatgagagcg cagcgccatg
      421 acccccgacg ctcgcgggcg tgccgatccg ggaccatcgc cggcggcggc aagccgcacc
      481 ctgccggcgg ccgccgccgg catcgctgcc gagatggccg gcttccagcg cgaaatgttg
      541 cgcttcgcgg tgctgcagtt gcgcgatcag gccagcgccg aagacgcggt gcaggaggcg
      601 atgctcgccg ccctgcaggg agccgaccgc tttgccgagc gggcaaagct caagacctgg
      661 gtgttttcga ttctcaggaa caagatcgtc gacatcatcc ggcggcgagt gcgcgagcca
      721 agctatcagg cgcccgaaac ggagatcgat gacgccgatt tcgacccgct gttcagcgcc
      781 aacgggcact ggcacagcga cagccgaccg gcggattggg gcgacccgga aaagagtttc
      841 gagaaccagc gtttctgggc gatcttcgat ggctgtctga accgcctgcc ggcagcgacg
      901 gcacgcgtgt tcatgatgcg cgagatgctc ggcctggaaa ccgacgaaat ctgcaccgaa
      961 ctgtcgatga gcagcaacaa ctgctgggtc gttctgcacc gggcgcgcat gggactgcgc
     1021 atgtgcctgg atcagcaatg gttcgacgcc ggagaacaga ccagatgatg aactgcaggg
     1081 aagccgtcaa actgatgtcg gaggagatgg atcatgatct ctccggcggc cggcgctggt
     1141 cactgcgcct gcatctgctg atctgcctcg gctgccggaa ctaccggcgg cagctgtcct
     1201 tcctgcgcca ggcctgtcga cacgaggtgg ctgtggacga cacgccgctg gccccgccac
     1261 ccacctgaaa ccggcgcgcg ccgctgcccg ccagggtcag gcgctacgcc gagcccgact
     1321 caagcgccga caatgccgcc atcgtcgcgt ctgacgacga tcgtcgccga gcgtggtcga
     1381 ccgccagtga cattggcaaa ctggctcgcc ggccaacccg atccagccag cggcctggtc
     1441 gggtcggagc ggccgccggg cagctcgccc ggatgctgca cattgatgaa caggttgcgg
     1501 ccgtccggcg ttgcggtcgg cccggtgatc tcgcagcccc tgggcccggt gagaaaacgc
     1561 ctgacttcgc cgctgaccac atcggcgacc agcaactggt tgttgccgag gcgcgcgtag
     1621 tcgccacgat gcatggcact gccggaaacg tcggtggcaa tccacaagcg gccgtcacgg
     1681 tcgaaccaca ggccatccgg gctgccgaag gcgtcgccgc ggctgttgcc ccgcttctcc
     1741 gggtcggcgt gctgcgggtc cccaccgagc acgaagaggt cccagccgaa gctgccggca
     1801 gccgcgtccc ccccgtcctc gcgccagcgg atgatgtgcc cgaaaacatt cccgcggcgc
     1861 gggttggcgg cgtcgacgtc gacccgctga cggttgttgg tcaagctgca ataaacctcg
     1921 ccgcttccgg gatggaccgc cagccattcc ggacgatcca tcttcgtcgc cccgacgaga
     1981 tcggccgcct gacgggtgcg gatcaggata tcggcctggt cggcaaagcc attggcctcg
     2041 gtgaggccgt tctgcccgtg ttgcagcgca atccagtgac cccgcccgtc ggcgccgaaa
     2101 cgggcgacgt gcagggtccc gatcgcgagc aggctaccgc cggcggcttt gccggcgtcg
     2161 aatcgatccc gggagacata cttgtagagg tattcgaagg cctcgtcgtc acccatgtag
     2221 accaccaccc ggcgatcgcc ggccagcgtg agcattgccc cttcgtgctt gaaacgcccc
     2281 agcgccgtgt gcttgaccgg gacgctggca ggattccacg ggtcgatctc gaccacccag
     2341 ccgaaacgat tggcctcgtt tggctcgctg gccaagtcga aacggcggtc atgctcgtgc
     2401 cagcgcattc cggcgccccg cgcacgcagg ccgtaacgct gctgcgccgc ggtcggctgg
     2461 tcgagcccgt tgaaaaaggc gttgaaattc tcctcgcaac tcaggtaggt gccccagggg
     2521 gttacaccca tcgcgcaatt ggcgagcgta ccgaaaacga catcgccacg ccggtcatca
     2581 cgggtgcgca tggcggctgc gccggccgcc ggaccgtcga tgcgcatcgg cgtgcgcgcg
     2641 gtaatccgcc gcgcgtatgc cgaaggccgc agcacccgcc aggcgaggtc cctgccgctc
     2701 cccgccagtt cgacttcgat caccgaaaca ccgtgcgcat gctgcgactt gagcatcttg
     2761 gcatgactcc agtcggccat cccatccggg tgcagcaggc cctcgtcggt gtattcgtgg
     2821 ttgacgcaca acaggccgtg cgtggacgac ggctggccat gcctgacgaa gggaaagaaa
     2881 tgcatgccat cgtgatgcat tccggcctgc tcggcctgtt ccgcggcact gttgccggca
     2941 tcgccccgga atcccggagc gtccgaaacc gggtcacccc aggcgtagag catctgcacg
     3001 tggtagccct cggggacgcg cacacggtct tcactcgacg ccgccagagg cgggaagcgc
     3061 atgccgagct gagcacccgc cgcgacccgc agcggcaact ccaggaacgg cagggccgcg
     3121 ctgccgaaac ccagttgcag gaagcgacga cgcctcgccg acagcgtcga agacgcgacc
     3181 agaagttgct cgaagtgcgg gttcgtgctg tcgttgctga tcggatcgtc gggcatgacc
     3241 ggcggtgctt tctcgtgagc ggaagggcaa attatcgcgc agggaagcgt cgtcatggcg
     3301 acgccgcgga tcggttggga agtccgtgcc gaacgtggtg cggccaccga cgagtccagc
     3361 gctcctggtc ggacgggatg ctgccgggag tgccgaaaat cccgcgacat ttcgcaacag
     3421 tgttgcattt ggatggcgaa cgaggcaaga tccgcgctct tcatcg
//
LOCUS       DAOSIT010000218         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23802_length_3460_cov_8.251689, whole genome shotgun sequence.
ACCESSION   DAOSIT010000218 DAOSIT010000000
VERSION     DAOSIT010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23802_length_3460_cov_8.251689"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3460
                     /locus_tag="PLL14_06355"
     CDS             <1..>3460
                     /locus_tag="PLL14_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03582.1"
                     /translation="ALLRTPIRQEISATAARTSQTLMGSLGLLVLALLLVHAWLRSRA
                     ERATTDRHAAASGVAALVLLIGLTLTYGLFTEWRERSRQALTRDFQAAAAERAERVRA
                     SIENNLDEIETVRRFFAGVASVSRQQFSTFVTPTLERHRFQALEWLPRVPHAQRAAYE
                     AAARRDHLEDFQFTESNAEGRLVRAGDRAEYFPVYYLEPHAGNAAALGFAPGPEHPAR
                     GAPLAKARDQGQLTATARYILVQDRAGSERYSILVFAPVYHGLPMPAEVDQRRRHLRG
                     FVLGVLRVGDMVSDALQGAEPGQLSFRLLDLTSGSEEGWLYDHPPGGAFPAATTSDLR
                     FSRDFPLADRFWRIEVAPSAAFVARHHDQSYRWIPAGGGLLTLLAAMYLFALVAQRQR
                     AEQLVAERTIALRASEAQFRQIIEHAPFGFHLYRLTADDRLLLGGANPAAEAMLGISQ
                     AQCMDRPVEEILPGLLASEIPNACRQLARTGGARRWERVTYGQPEKDESFEMVAFQSA
                     PGLVAVAFADITERRRAETALHDSEQRFRAMIEHAPDGIALFNGSIRYASPAVSHLLG
                     YTPEEAMALDPEQATHPDDLPWLLPLLDELTRRPGDVVSARYRFRHKDGSWRWLESTI
                     SNLLHEPAVNALVFNFRDVTERQQAEAHLRLAAAVFEAARDAIMVTDSKWNIVAVNPA
                     FSTLTGYAECEVRGHKPLLLWAERQPSAYFESMARTLAVAGVWQGEFWARRKDGERRA
                     ALASLGAVRDANGTISHHVGVATDITALKIAEQRIEHQAYYDALTDLPNRSLLAQHAE
                     LALARATRQRSALALLFLDLDQFKEVNDSLGHAEGDALLVQAAARLKEITRGEDTVCR
                     LGGDEFVLLLPGTAQDGALRVADKLLTAFRQPFMVAGHPLRTTVSIGIALFPNDGTSF
                     AELLKNADTALYQAKQEGRNTRVFYDRQMNAAILQRLVLESELRQALASGQLRAYYQP
                     KIRLSDGQTVGAEALLRWQHPEDGMVPPGRFIPVAETSDLIVELGVWMLGEVCRQLAS
                     WRADGLPSLPVAVNLAARHFRQPDLANCIRGLLEASALPAQALELELTESSLLEAGPQ
                     TAETLQALEELGVGLAIDDFGTGYSSLSYLKRLPLSALKIDQSFVRDLVSDPDDRTIA
                     AAIVALG"
BASE COUNT          575 a         1199 c         1129 g          557 t
ORIGIN      
        1 tggcactgct gcgcacaccg atccgacagg agatctccgc gaccgccgcg cgcaccagcc
       61 agaccctgat gggttccctc gggttgctgg tgctggcgtt gctgctggtg catgcctggc
      121 tgcgttcgcg cgccgaacgt gccaccacgg atcgccacgc cgccgcctcg ggcgtggctg
      181 cgctggtgct gctgatcggg ctgaccctga cctatggcct atttaccgaa tggcgagagc
      241 gcagccggca ggcgctgacc cgagatttcc aggccgcagc tgcggaaagg gccgaacgcg
      301 tgcgcgcgtc gatcgaaaac aacctcgacg agatcgaaac cgttcgacgc ttcttcgccg
      361 gcgtcgccag cgtcagtcgt cagcagttca gcacgtttgt gacgccaacg ctggagcggc
      421 acaggtttca ggcgctggag tggctcccgc gcgtgccgca cgcgcagcgc gccgcctacg
      481 aggccgcagc ccgccgggac caccttgagg atttccagtt taccgaaagc aatgccgagg
      541 gccggctggt acgcgccggc gaccgcgccg agtatttccc cgtttattac ctggagccgc
      601 acgccggcaa tgcggcggcg ctgggtttcg cccccggtcc ggagcatccg gcgcgtggcg
      661 cgcccctcgc caaggcgcgc gaccaggggc aactgacggc caccgcgcgc tacatcctgg
      721 tgcaggaccg ggccggcagc gaacgttatt ccattctggt gtttgcaccg gtctaccacg
      781 gtctgccgat gccggccgag gtcgatcagc gccgtcgtca cctgcgcggc ttcgtgctcg
      841 gcgtcctgcg tgtcggcgac atggtcagtg acgcactgca gggtgccgaa cccgggcagt
      901 tgtcgtttcg tctgctcgat ctgacgtccg gcagcgagga aggctggttg tacgatcatc
      961 cgccaggcgg cgctttcccg gcagcgacga ccagcgacct gcggttctcc cgtgactttc
     1021 ctctcgccga ccgcttctgg cgcatcgaag tggcgccgag cgctgccttc gttgcccgtc
     1081 accacgatca gagctaccgc tggattccgg ccggcggcgg cctgctcacc ctgctcgcgg
     1141 cgatgtatct gttcgctctg gtggcgcaac gccagcgtgc ggaacaactg gtcgccgaac
     1201 gcaccatcgc gttgcgcgcc agcgaggcgc agttccgcca gatcatcgag catgccccgt
     1261 tcggcttcca tctctaccgc ctgacggcgg atgaccggct gctgctcggc ggcgccaatc
     1321 cggcagccga agcgatgctc ggcatcagcc aggcgcagtg catggacaga ccggtcgagg
     1381 agatactccc gggcctgctg gccagtgaaa ttcccaacgc ttgccgccag ctggcgcgca
     1441 cgggcggggc acgacgctgg gagagggtaa cgtatggcca gccggagaag gatgaaagct
     1501 tcgagatggt ggccttccag agcgcgccgg gtctggtcgc cgtggccttc gccgacatca
     1561 ccgaacgacg gcgtgcggaa acggcgctgc acgacagcga gcaacgtttt cgggcgatga
     1621 tcgagcacgc tcccgacggc attgccctgt tcaacggcag catccgctat gccagcccgg
     1681 cggtttccca ccttctcggc tacaccccgg aagaagccat ggctctggat cccgagcaag
     1741 cgacgcatcc cgacgacctg ccgtggctgc tgccgctgct ggacgaactg acgcggaggc
     1801 cgggagatgt ggtctcggcg cgctatcgct ttcgccacaa ggacggctcc tggcgctggc
     1861 tggagagcac gatcagcaac ctgctgcacg agccggcggt gaacgcgctg gtcttcaatt
     1921 tccgcgacgt gaccgaacgt cagcaggccg aagctcacct gcggctggcg gcggcagtgt
     1981 tcgaagcggc gcgcgacgcg atcatggtca ccgactcgaa gtggaacatc gtcgccgtca
     2041 accccgcctt cagcaccctc accggctatg ccgaatgcga ggtgcgcgga cacaagccac
     2101 tcctgttgtg ggccgaacgg caaccatcgg cgtatttcga gagcatggcg cggaccctgg
     2161 ccgtcgcggg cgtatggcag ggtgagttct gggcccggcg caaggacggc gagcgtcgcg
     2221 ccgcgctcgc cagcctgggg gcggtgcgcg atgccaacgg cacgatcagc caccacgtcg
     2281 gcgtggccac cgacatcacc gccctcaaga ttgccgaaca gcgcatcgag caccaggcct
     2341 actacgacgc gttgaccgac ctgccgaacc gctcactgct ggcacagcac gcggaactgg
     2401 cgctggcccg ggcaacacgc cagcgcagcg cgctggcgct gctgtttctc gacctggacc
     2461 agttcaagga agtcaacgac tcgctcggac acgccgaggg cgatgccctg ctggtacagg
     2521 cggcggctcg cctgaaggag atcacccgtg gcgaggatac ggtctgtcga ctgggcggcg
     2581 acgagttcgt gctgttgctg ccgggtaccg cccaggacgg tgcgttgcgg gtggccgaca
     2641 agttgctgac ggcgttccgc caacccttca tggtggccgg ccatcccctg cggaccacgg
     2701 tatcgatcgg catcgccctt ttcccgaacg acggcaccag cttcgccgaa ctgctgaaaa
     2761 acgccgacac cgcgctctac caggccaaac aggagggtcg caacacgcgc gtgttctacg
     2821 accggcaaat gaacgccgcc atcctgcagc gcctggtgct cgaaagcgag ttgcgccagg
     2881 cgctcgccag cggccagttg cgcgcttact accagcccaa gatacggctg agcgacggcc
     2941 agacggtcgg cgccgaagcg ctgctgcgct ggcagcaccc cgaggacggc atggttccac
     3001 cggggcgctt cattccggtc gcggaaacca gcgacctgat cgtcgaactg ggggtctgga
     3061 tgctcggcga agtctgccgg cagctggcga gctggcgcgc cgatggtctg ccgagcctgc
     3121 cggtagcggt caacctcgcc gcccgccact tccgccaacc ggatctcgcc aactgcatcc
     3181 gcgggctgct cgaagccagc gccctgcccg cgcaggcact ggagctggaa ctcaccgagt
     3241 cgagcctgct ggaagccgga ccgcagacgg cggaaaccct gcaggccctg gaagaactcg
     3301 gcgtcggcct ggccatcgac gacttcggca ccggctactc cagcctcagc tatctcaaac
     3361 gtctccccct cagcgccttg aaaatcgatc agagcttcgt ccgcgatctg gtcagcgatc
     3421 ccgatgaccg caccatcgcc gcggccattg tcgcccttgg
//
LOCUS       DAOSIT010000219         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23869_length_3451_cov_10.272379, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000219 DAOSIT010000000
VERSION     DAOSIT010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23869_length_3451_cov_10.272379"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..946)
                     /locus_tag="PLL14_06360"
     CDS             complement(131..946)
                     /locus_tag="PLL14_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOG03583.1"
                     /translation="MNDLAAPITGQEFALFQRLIYRLAGISLSDAKRVLLVGRLGRRL
                     KHYGFDTYTQYYRLLASGEHPDEVQQMIDLLTTNETYFFREPQHFDFLRDVALKERRG
                     PGTFRIWSGASSSGEEAYSMAMVLAEHRSDGPWEVFGSDISMTVLARARAGIYTQERI
                     SGIPPAYLRKYCLKGVRSHEGKLLIDNALKQRVSFAQVNLTLPISGVGQFDVIFLRNV
                     MIYFDAETKRKVVENMLPSLKPGGYFIVGHSESLNGITTRLKTLKPTIYQQAL"
     gene            complement(1045..1605)
                     /locus_tag="PLL14_06365"
     CDS             complement(1045..1605)
                     /locus_tag="PLL14_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOG03584.1"
                     /translation="MNNPRDLERLARNVNPGGWAIENQRPIATLLGSCVAVCLWDPQL
                     RIGGLNHFMLPKFEKTANKDLDVLLCGNFCMEALLNGMLARGAQKKRLQGKAFGGGNV
                     VSSLTGVSIGQRNAEFAREWLAREGVPLLASDLMGPWSRKVLLDPQTGDAFCKRGHTN
                     QSIAEAEKAYASTLLVAPKKADIELF"
     gene            complement(1602..>1813)
                     /locus_tag="PLL14_06370"
     CDS             complement(1602..>1813)
                     /locus_tag="PLL14_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03585.1"
                     /translation="TSDANAAEIASTIIAMARSLQLSVLAEGVETPAQLAFLERHGCE
                     YYQGYFFSRPVAAAQFPESCPVPSP"
     assembly_gap    1814..1913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1914..>3451)
                     /locus_tag="PLL14_06375"
     CDS             complement(<1914..>3451)
                     /locus_tag="PLL14_06375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG03586.1"
                     /translation="LDADGKPCDYRFIDVNPAFEAITGLARADTVGRCVRELLPDIEP
                     HWIERYGAVALTGDGQQFDDFSSDLDRQYRVTVYRPEPQQFAVIVDDITALRRHEESL
                     RQAATVFEQTRDGVAITDASARIIAVNRAFSKITGYSREEVVGRNTSLLKSGHHDAAF
                     YAAMWEQLTSKGFWEGEVCNRRKNGEHYPQWLTISAVLNERNEIERYIGVFTDISRIR
                     NAEAHAEFIAYHDALTRLPNRSLLNIRYQHALDRARRHDHKLALLILDLDLFRNINDS
                     LGHEFGDRLLVAITERFKSRLRDEDTLARLHGDEFAVLMEDVEVAADAAVVARDLLKS
                     LEAPFRIGSAEDIYIGASIGISISPDDGCDPALLMRNADTAVNLAKAQGRKTFCFYTE
                     SLTTLALERMRLESSLRKAIELEHLVLHYQPLIEARTGAVIGSEALVRWPDPEQGMIA
                     PMRFIPVAEDCGLIAPLGAWVLLTACRQMKAWLDAGRPLRTIAVNVSPRQFFLQDVPE
                     LVRQT"
BASE COUNT          583 a          973 c         1133 g          662 t
ORIGIN      
        1 gagcggctgc tggcggcgtg caggcggttg agcaggcaga ccatggcgcg atggtctgtg
       61 tcgatctcgg ggtgcccgac ttccagtgct gccgtccatt caagatgcac catgtcgtga
      121 tcctcggagt tcaaagtgcc tgctgataga tcgtgggctt caaggttttc agccgggtgg
      181 tgatgccgtt cagcgattcc gaatggccga cgatgaaata gccgccgggc ttgagactcg
      241 gcagcatgtt ttcgacgacc ttgcgcttgg tttcggcgtc gaaatagatc atcacgttgc
      301 gcaggaagat gacgtcaaac tgcccgacgc cgctgatcgg caaggtcagg ttgacctgtg
      361 cgaagctgac gcgctgtttg agcgcgttgt cgatcaggag cttgccttcg tgcgagcgca
      421 cgcccttgag gcaatacttg cgcaggtaag ccggcgggat tccggaaatc cgctcctgcg
      481 tgtagatgcc ggccctggcc ctggccagca cggtcatgct gatatccgac ccaaaaacct
      541 cccaagggcc gtcactgcga tgctcggcca gtaccatggc catcgaatag gcctcttcgc
      601 cactcgaact ggcgccgctc cagatgcgga aggttcccgg cccgcgccgc tccttgagcg
      661 ccacgtcgcg caggaagtcg aaatgctgcg gctcacgaaa gaaataggtt tcgttggtgg
      721 tcagcagatc gatcatctgt tgcacctcgt ccggatgttc gccggaggcc agtagccggt
      781 aatactgggt gtaggtatcg aagccgtagt gcttgagacg gcgaccgagg cgtccgacga
      841 gcagcacgcg tttggcatcc gacaggctga tgccggcgag tcggtagatc aggcgttgga
      901 agagcgcgaa ctcctggccg gtaatcgggg cagcgaggtc gttcattggc gtgcctcggc
      961 agttcgcagc tgcggctgcg agcgtagcgg atgtggggga atgttcatga ccgttctccg
     1021 tgcgtgtggg ccattcctgg accgctagaa aagctcgatg tcggccttct tcggcgcgac
     1081 caggagggtg ctggcgtaag ctttttcggc ttcggcgatg ctctggttgg tgtggccccg
     1141 cttgcagaag gcgtcgccgg tctgcggatc gaggaggacc ttgcgcgacc acgggcccat
     1201 caaatccgag gcgagcagtg gcacgccctc gcgcgccagc cattcgcgcg cgaactcggc
     1261 gttgcgctgg ccgatgctga cgccggtgag cgaagagacg acgttgccgc caccgaaggc
     1321 cttgccctgg aggcgcttct tttgcgcacc gcgggcgagc atgccgttga gcagcgcttc
     1381 catgcagaaa ttgccgcaga gcagcacgtc gaggtccttg ttggcggtct tctcgaattt
     1441 cggcagcatg aaatggttca agccgccgat ccggagttgc ggatcccaca ggcagaccgc
     1501 gacacacgag ccgaggaggg tggcaatcgg gcgttggttc tcgatcgccc aaccgcccgg
     1561 attgacgttg cgcgccaggc gttcgagatc gcgtggatta ttcatggcga gggtaccggg
     1621 cacgattcgg gaaactgcgc cgccgcgacc gggcggctga agaaatagcc ttgatagtat
     1681 tcgcagccgt gccgttcgag aaaagccagt tgggcgggcg tctcgacgcc ttcggcgagc
     1741 accgagagtt gcagtgaacg ggccatggcg atgatcgtcg aagcgatttc ggcggcattg
     1801 gcgtcactgg tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtctggc
     1921 ggacgagttc cgggacgtcc tgcaggaaga actggcgcgg cgagacattg acggcgatgg
     1981 tccgcaaggg tcgcccggca tcgagccagg ctttcatctg ccgacaggcg gtcagcagaa
     2041 cccaggcgcc aagtggcgcg atcagcccgc aatcctcggc caccggaatg aagcgcatcg
     2101 gcgcaatcat tccctgttcc gggtctggcc agcgtaccag cgcttccgag ccgattaccg
     2161 cgccggtcct ggcctcgatc agcggttgat aatgcaggac cagatgctcg agttcgatgg
     2221 ctttgcgcag gctggactcc agccgcatgc gttcgagcgc cagcgtggtc agcgattcgg
     2281 tatagaagca gaatgtcttg cgtccttgcg ccttggccag gtttaccgcg gtgtcggcgt
     2341 tgcgcatcag cagcgccggg tcgcagccgt cgtcgggaga gatgctgatc ccgatgctgg
     2401 caccgatgta gatgtcctct gcgctaccga tccggaaggg cgcttcgagc gacttgagca
     2461 ggtcacgcgc gacgactgcg gcgtcggcgg ccacctcgac gtcttccatc agcaccgcga
     2521 actcgtcgcc gtgcaggcgt gccagggtgt cctcatcgcg cagacgagac ttgaagcgtt
     2581 cggtgatcgc caccagcaga cggtcgccga attcatgtcc cagcgagtcg ttgatatttc
     2641 tgaagaggtc gaggtcgagg atcagcaagg ccagcttgtg gtcgtgacgg cgtgcgcgat
     2701 cgagggcgtg ctggtagcgg atgttcagca ggctgcggtt gggcaggcgt gtcagggcgt
     2761 cgtggtaagc aatgaactcg gcatgggcct cggcgttcct gatgcggctg atgtccgtaa
     2821 acacgccgat gtagcgttcg atttcatttc gctcattgag cacggcgctg atcgtcagcc
     2881 actgcggata gtgttcgccg ttcttgcggc ggttgcacac ctctccttcc cagaagccct
     2941 tgctcgtcaa ctgctcccac atggcggcgt aaaacgccgc gtcatgatgg ccggatttga
     3001 ggaggctggt attgcgaccg acaacttctt cgcggctata gccggtgatc ttgctgaacg
     3061 cgcgattgac ggcaatgatc cgggccgagg cgtcggtgat cgccaccccg tcgcgggtct
     3121 gttcgaatac cgtggccgcc tgccgcaggg attcctcgtg gcggcgcaag gcggtgatgt
     3181 cgtcgacgat cacggcgaac tgctgcggtt ccggtcggta gacggtgacc cggtactggc
     3241 ggtcgaggtc gctgctgaaa tcgtcgaact gttgaccgtc gccggtcagg gcgacggcgc
     3301 catagcgctc gatccagtgc ggctcgatgt ctggcagcaa ctcgcggacg cagcgcccga
     3361 cggtgtcggc cctggccagg ccggtgatcg cctcgaacgc cgggttgaca tcgatgaagc
     3421 ggtaatcgca aggcttgccg tcggcatcga g
//
LOCUS       DAOSIT010000220         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_23984_length_3438_cov_10.925510, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000220 DAOSIT010000000
VERSION     DAOSIT010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23984_length_3438_cov_10.925510"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /locus_tag="PLL14_06380"
     CDS             <1..2163
                     /locus_tag="PLL14_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019472290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HOG03587.1"
                     /translation="VMYALGLLSDEAIENGFAGPTLGFEFAGIVSRVGCEGGQFSVGD
                     EVVGFGPSSFGNRVVTETGAIAHIPPGMSCEAAATIPSTFFTVYYALHYLARLQPEEK
                     VLIHGAAGGVGIAAIQVAKWIGAEIYATAGSDEKRDFLRLLGVEHIFDSRSLAFADQI
                     LEQTGGQGVDVVLNSLAGEAINRNFQVLKPFGRFLELGKRDFYENTKVGLRPFRNNIS
                     YFGIDADQLMLVHPELTRRLFAEIMALFGEGALHPLPYHTFEAEDIVDAFRYMQQARQ
                     IGKIVITYHHGITRAQPPVAVVRKSLRFPPDASYLITGGLRGFGLRTAEWLADKGARH
                     LILISRSGTASSEARAGIERLQERGVKVHAAACDVTSREALAGLLTETARTMPPLKGI
                     VHAAAVIDDRLVRNIDSQQIRSVLGPKVLGARHLHDLTRTQELDLFVLFSSATTPFGN
                     PGQASYVAANACLEALAEHRRSIGLCATCIGWGPIDDVGFLARHEKTKEALESRMGGS
                     AINSAIALDALEGMLLADRSGLAVLELDWQALSRFLPTAASPKFRELAWHVGNADSQE
                     DHSEDIQRLLAELPDDELLAAFIDLLKTEIGEILRVAPDKIDPNRSLYDMGLDSLMGV
                     ELMIALETRFGIRLPVMALSQSPSIAKLAKRVIQQLRGNEEGSEESSDAQAHRGIVAQ
                     AEQVARQQGVEASLESIASLADDLQSATSTAAAGRRSD"
     gene            2165..>3438
                     /locus_tag="PLL14_06385"
     CDS             2165..>3438
                     /locus_tag="PLL14_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03588.1"
                     /translation="MARKGLPGLTAQIKDRLIQQALERRLRQAEQGQGMTALTRSPSG
                     STDTPEQHYRFDLHPGYQQIRIISEGAHRLGIDSPFFKLHEGIAGATTRIDGREYVNY
                     ASYNYLALSGHPEVAAAARQAIDQYGTSVSASRLVSGERPVHRELERAIASAYGVDDA
                     ITFVSGHATNVTTIGHLFGPQDLILHDELIHNSVVQGISLSGARRLPFPHNDWAALDA
                     ILGQQRHHFQRVLIVLEGIYSMDGDYPELPRFVELKRKYRAFLMVDEAHSFGVMGRSG
                     LGIREHFGLPSADVDIWMGTLSKALAGCGGYIAGEAALVEHLKFLAPGFLYSVGMPPA
                     IAAASLAALQCLQREPQRVATLQERGRLFLAQAQAAGVDTGTSAGFAVIPAITGSSIK
                     AARLASALFERGINVQPILYPAVPEKSARLRF"
BASE COUNT          646 a         1143 c         1044 g          605 t
ORIGIN      
        1 gtcatgtacg cgttgggact gctttccgac gaggcgatcg agaacggttt tgccggaccg
       61 acgctgggct tcgagtttgc cggcatcgtc agtcgtgtcg ggtgcgaggg tgggcaattc
      121 agtgtcggcg atgaggtggt cgggttcggg ccgtcgagct tcggcaaccg cgtcgtgacc
      181 gaaaccggag cgattgcgca cattcccccc ggcatgtcct gtgaagccgc ggcgacgatc
      241 ccgagcacct tctttaccgt ctactacgcc ctgcactacc tggcacgcct gcaacccgaa
      301 gaaaaggtgc tgattcatgg cgctgcgggc ggcgtcggaa tcgccgccat ccaggtcgcc
      361 aagtggatcg gtgccgagat ctatgccacc gccggatcgg acgaaaagcg cgacttcctg
      421 cgcctgctcg gtgtggaaca catttttgac tcgcgctccc tggcctttgc cgaccagatc
      481 ctcgagcaga ccggcggtca gggcgtggat gtcgtcctca attcgcttgc cggcgaagcg
      541 atcaaccgga atttccaggt gctgaagccg tttggccgct ttctcgaact tggcaagcgc
      601 gatttctacg agaataccaa ggtcggcttg cgtccgttcc gcaacaacat cagttatttc
      661 ggcatcgacg ccgatcagtt gatgctggtc caccccgaac tgacacgtcg cttgttcgcc
      721 gagatcatgg ccctgtttgg agaaggggct ctgcatcccc tgccctacca caccttcgag
      781 gcggaagata ttgtcgacgc ctttcgctac atgcaacagg cccgccagat cggcaagatc
      841 gtcatcacct atcatcacgg cattacgcgc gcgcagccgc cggtggccgt agtgcgcaag
      901 tcactgcggt tcccgcccga tgcttcgtat ctgatcaccg gcggcctgcg cggtttcggc
      961 ctgcgtaccg ctgaatggct ggccgacaag ggcgcgcgcc acctgatcct gatcagccgc
     1021 agcggaaccg cgtccagcga agccagggcg ggcatcgagc gcctgcagga acgaggcgtc
     1081 aaggtacatg ccgccgcctg cgacgtgacc agccgcgaag cactcgccgg gttgctcacc
     1141 gaaacggcca ggaccatgcc gccgctcaag ggcatcgtgc atgctgcggc ggtgatcgac
     1201 gaccgactgg tacgcaacat cgattcccaa cagatacgca gcgtgctggg cccgaaagtc
     1261 ctcggcgccc gccatctgca cgacctgacc cgcacgcagg aactcgatct gttcgttctc
     1321 ttttcgtcgg caaccacgcc tttcggaaat cctggacaag ccagctacgt cgcggccaac
     1381 gcctgcctgg aagcgctggc cgagcatcgc cgcagtatcg gcctgtgtgc gacctgtatc
     1441 ggctggggtc cgatcgacga cgtgggtttc ctggcacgcc acgagaagac caaggaagcg
     1501 ctcgaaagcc gcatgggagg atcggcgatc aactcggcaa tcgccctcga cgccctcgaa
     1561 ggcatgctgc ttgccgaccg tagcggactg gcagtcctgg aactcgactg gcaggcgctg
     1621 agccgctttc ttcccacggc ggcgagcccg aagtttcgcg aactcgcctg gcacgtcggg
     1681 aatgccgata gccaggagga ccattcggag gacatccagc ggctgctggc ggaactcccc
     1741 gacgatgaac tgctggccgc cttcatcgac ctcctcaaga ctgagatcgg agaaatcctg
     1801 cgcgtcgccc cggacaagat cgatcccaac cgctcgcttt acgacatggg tctggactcg
     1861 ctgatgggag tcgaactgat gatcgcgctc gaaacgcgtt tcggaatccg cttgccggtg
     1921 atggcgctga gccagagtcc cagcatcgca aaactcgcca aacgcgtcat tcagcaactg
     1981 cgaggtaacg aggaaggcag cgaagaaagc agcgacgccc aggctcatcg cggcatcgtt
     2041 gcacaggctg aacaggtcgc caggcagcag ggcgtcgagg cctcactcga gtcgatcgcc
     2101 agcctcgcgg acgacctcca gtcggcaaca agcactgccg ccgctggccg gaggagcgat
     2161 tgacatggca cgcaagggct tacccgggct gaccgcccag atcaaggaca gactcatcca
     2221 gcaagccctc gaacgccggc tccgtcaggc cgagcaggga caagggatga cggcgctaac
     2281 gcgttcgccg agcggaagta ccgacacccc cgagcagcac taccgctttg atctgcatcc
     2341 gggctaccag cagattcgca tcatcagcga aggcgcgcac cgcctgggca tcgacagtcc
     2401 cttcttcaag ctccacgaag gcatcgccgg ggcgacgacg cgcatcgacg gacgcgaata
     2461 cgtcaactac gcgagctaca actacctcgc gctttccggc cacccggagg ttgccgcggc
     2521 ggcccgacag gccatcgacc aatacggcac ctcggtttcc gcaagccgcc tcgtttccgg
     2581 cgaacgaccg gtacaccggg aactcgaacg ggccatcgcc tcggcctatg gggttgacga
     2641 tgcgatcacc ttcgtcagtg gtcacgcgac caacgtcacc accatcggcc acctcttcgg
     2701 ccctcaggat ctgattcttc acgacgaact gattcacaac agcgtcgtgc agggaatcag
     2761 cctttcaggt gcccgccgcc tgcctttccc gcacaatgac tgggctgcac tggacgccat
     2821 cctcggccag caacgccacc actttcagcg ggtgctgatc gtgctggagg ggatctacag
     2881 catggatggc gattatccgg aactgccccg cttcgttgag ctcaagcgca agtaccgagc
     2941 cttcctgatg gttgacgaag cccactcgtt cggcgtcatg gggcgcagcg ggctcggcat
     3001 tcgggagcac ttcggactgc ccagcgccga cgtcgacatc tggatgggaa ccctgagcaa
     3061 ggcactcgcg ggttgcggcg gctatatcgc cggcgaggcg gcactggtcg aacatctcaa
     3121 gttcctggcc ccgggtttcc tgtacagcgt cggcatgccg ccggcgattg ccgccgcctc
     3181 gctggcggcc ctgcaatgcc tgcagaggga gccccagcgc gtcgccacgc tgcaggagcg
     3241 cgggcgtctt ttcctcgcgc aagcccaggc ggccggcgtc gacaccggca ccagtgccgg
     3301 attcgccgtg atcccggcaa ttaccggcag ttcgatcaag gcggcgcgcc tggcgtcggc
     3361 acttttcgag cgtggcatca acgtgcagcc gatcctctat ccggccgtgc ccgagaaatc
     3421 cgcgcgtctg cgcttctt
//
LOCUS       DAOSIT010000221         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24017_length_3434_cov_12.534478, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000221 DAOSIT010000000
VERSION     DAOSIT010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24017_length_3434_cov_12.534478"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLL14_06390"
     CDS             complement(<1..941)
                     /locus_tag="PLL14_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOG03589.1"
                     /translation="MASPQDVINMVKENEAKFVDFRFTDTRGKEQHVTVPVSAFDEDK
                     FENGHAFDGSSIAGWKGIQASDMQLMPDPSTAYIDPFFDETTLVLTCDVVDPADGRGY
                     DRDPRSIAKRAEAYLKSCGIGDTAYFGPEPEFFIFDSVSWSVDMSGCTLKIFSQEAAW
                     TSGEKTEGEGGTGHRPGIKGGYFPVPPVDSLHDIRSAMVLTLETVGVPVEVHHHEVAT
                     AGQCEIGTLFSTLVKRADWTQVLKYVVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQS
                     IWKDGKNLFAGSGYAGLSEMALFYIGGIIKHAKALNA"
     gene            1233..1682
                     /locus_tag="PLL14_06395"
     CDS             1233..1682
                     /locus_tag="PLL14_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG03590.1"
                     /translation="MGKLTDLLQLAQERGRELGLPYAGALTPQEAHAVWRLAPGAKLV
                     DVRTRAEWDWVGRIPGAIEIEWMSYPDNRPNSHFLSQLKQQVDREALLMFICRSGARS
                     HNAAALVGEALRNDCYNVLEGFEGDKDANGQRGQLGGWRHAGLPWRS"
     gene            1738..2835
                     /gene="nadA"
                     /locus_tag="PLL14_06400"
     CDS             1738..2835
                     /gene="nadA"
                     /locus_tag="PLL14_06400"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOG03591.1"
                     /translation="MQTSTISFDSFNHLQDDACHQRIRVARERLGREAVLLCHHYQRA
                     DVYQYADLTGDSLKLSRLASQTDSRYIVFCGVHFMAEVADIMSKPEQVAILPDLAAGC
                     SMADMANLAKVERCWRELGEVLDAEQEVTPVTYINSAADLKAFCGAHGGIVCTSSNAG
                     TIAAWAFERRQKILFFPDQHLGRWTGFKMGIPLDEMMVWDPDLENGGLTSEQIRRARL
                     LLWKGHCSVHQMFQPAHIMRFRNHYPEGLVIAHPECSFEVCRQSDFVGSTEHIVRTIK
                     EAAPNTRWLVGTELNLVNRLAEEVRHEGKIVQFMAPTVCMCSTMQRIDPQHLAWTLEN
                     LADGKVVNQIRVPEHEKDLARLALDRMLAVS"
     gene            2855..3232
                     /locus_tag="PLL14_06405"
     CDS             2855..3232
                     /locus_tag="PLL14_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HOG03592.1"
                     /translation="MHGKLQQVVRIYGIRNCDSMKKAFVWLAEHAVVCDFVDYRQAAP
                     SAAQLADWNLRVGWKALLNTRGTTWRRLSKEDRADVDEARALTLMARHPSLIRRPVID
                     TGTALLVGFDPDRYAEELLGKGP"
     gene            3229..>3434
                     /locus_tag="PLL14_06410"
     CDS             3229..>3434
                     /locus_tag="PLL14_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOG03593.1"
                     /translation="MTGTMTDDCLHRFLFDDLDIRGALVRLGPAWRAMIENRAYPAPV
                     AQLLGEMSATTVLLGGQLKQPGRLT"
BASE COUNT          668 a         1026 c         1071 g          669 t
ORIGIN      
        1 atcgcgttca gcgccttggc gtgcttgatg atgccgccga tatagaagag cgccatttcg
       61 gaaaggccgg cataaccgct gccggcgaac aggttcttgc catctttcca gatcgactga
      121 tggacgtgca tgcccgaacc attgtcgcca acgatgggct tcggcatgaa ggtcgcggtc
      181 ttgccgtatt gatgagcgac attgtggacc acatacttga gcacctgcgt ccagtcggcg
      241 cgcttgacca gcgtgctgaa cagggtgccg atctcgcact ggcccgccgt ggcgacttcg
      301 tggtggtgca cttcgaccgg gacgccgacc gtttcgagcg tcaggaccat cgccgagcgg
      361 atgtcgtgca ggctgtcgac cggcggcacc gggaagtagc cgcccttgat ccctggacgg
      421 tgaccggttc caccctcacc ttcagttttt tcgccactcg tccaggcggc ttcctgcgag
      481 aagatcttca gggtgcagcc cgacatgtcg acgctccagg acaccgaatc aaagatgaag
      541 aattcgggtt ccggtccaaa ataagcggtg tcaccaatgc cgcaggactt caggtaggct
      601 tcggcacgct tggcgatcga ccgcggatcc cggtcgtagc cacggccatc ggcaggatca
      661 accacgtcac aggtgaggac gagcgtcgtc tcatcgaaga aaggatcaat gtacgcggtg
      721 ctcggatcgg gcatcaactg catgtccgaa gcctgaatgc ctttccagcc ggcgatcgac
      781 gaaccatcga aagcgtggcc gttctcgaac ttgtcttcgt cgaaagcgct gaccggcacg
      841 gtcacgtgct gttccttgcc acgggtgtct gtgaaacgaa agtcgacgaa cttcgcctcg
      901 ttttccttga ccatattgat gacgtcttgc gggcttgcca ttgcggagtc tcctgaacag
      961 gttgccggtt caaccggcga caatagaaca taagtgcgcg ggggtactag cagaaaacgt
     1021 gccaatccat gccctgctga gcgtttattg tgccacaagg gtctggcgca ttattgggct
     1081 tttttcaatg caccgcggcg gtgcgtacga atccgggcgg cacatttctg gtgcattgca
     1141 gggcgctggt tccgagcgcc tctcgggagt catgtgattt atactccttg cttcgataaa
     1201 ccctcttggg acaggctctc gccacacaca ctatgggaaa actgaccgat ctgctgcaac
     1261 ttgcgcagga acgcgggcgc gaactgggtc taccttatgc cggcgccctg acgccgcagg
     1321 aagcgcatgc ggtttggcgg cttgcgccag gcgccaaact ggtcgacgtc cgtacgcgcg
     1381 cggaatggga ttgggtcggc cgcattcccg gtgcgatcga gattgagtgg atgagctatc
     1441 cggacaatcg gcccaacagc catttcctgt cgcaattgaa acaacaggtc gatcgtgagg
     1501 cgctgctgat gttcatctgt cgcagcgggg cgcgttcgca caatgccgcc gcgctggttg
     1561 gcgaagcgct gcgtaacgac tgctacaacg tgctggaggg ctttgaaggc gacaaggatg
     1621 ccaacggcca gcgcggccag ctgggaggat ggcgtcacgc tggtttgcca tggcgtagct
     1681 gaaccttttg ccgccagccc ggtctggctt cctggacatc gatttgagta ttaccgcatg
     1741 caaacctcga ccatcagctt cgacagtttc aaccacctcc aggacgatgc ctgtcaccag
     1801 cgcatccgcg tcgctcgtga gcggctcggc cgggaggcgg tgctgctctg ccatcactac
     1861 caacgcgccg acgtgtatca atacgccgac ctgacgggcg actcgctgaa actctcgcgt
     1921 ctcgcctcac aaaccgattc ccgctacatc gtgttttgcg gtgtgcactt catggccgaa
     1981 gtcgccgaca tcatgtcgaa gccggagcag gtggcgatcc tgccggatct ggcggcgggc
     2041 tgttcgatgg cggacatggc caacctggcc aaggtcgaac gctgctggcg tgaactcggc
     2101 gaggtcctgg acgccgagca ggaagtcacg ccggtgacct acatcaattc ggccgccgat
     2161 ctcaaagcct tctgcggcgc gcacggcggc atcgtgtgca cctcgtccaa tgccggcacg
     2221 attgccgcct gggcattcga gcgtcgccag aagattctgt tctttcctga tcagcacctg
     2281 gggcgctgga ccggcttcaa gatgggcatt ccgctcgatg agatgatggt ctgggacccc
     2341 gatctcgaaa acggtgggct caccagcgag cagatccggc gggccagact gctgctctgg
     2401 aaaggccatt gctcggtaca tcagatgttc cagccggcgc acatcatgcg tttccgcaac
     2461 cactacccgg aggggctggt gattgcccat cccgagtgca gcttcgaggt gtgccggcaa
     2521 tcggatttcg tcggctcgac ggaacacatc gtccggacga tcaaggaagc cgcgccgaat
     2581 acgcgctggc tggtcggcac cgagctcaat ctcgtcaacc gtctggccga agaggtcagg
     2641 cacgagggca agatcgtgca gttcatggca ccaacggtct gcatgtgttc gacgatgcag
     2701 cgcatcgacc cgcagcacct ggcatggacg ctcgagaatc tggccgatgg caaggtggtc
     2761 aaccagattc gggttccgga gcacgagaaa gacctggcca ggctggcgct ggacaggatg
     2821 ctggccgtat cctgagacaa accgggggca gggcatgcac ggcaagctgc agcaggtcgt
     2881 ccgtatctac gggatcagga actgcgacag catgaagaag gccttcgtct ggctggcgga
     2941 gcatgctgtg gtctgtgact tcgttgacta tcgccaagcg gcaccaagcg ccgcgcaact
     3001 ggccgactgg aacctccgcg tcggctggaa agcgctgctc aacacgcgcg ggacgacatg
     3061 gcggcggctc agcaaggaag accgtgccga cgttgacgaa gcccgggcgc tgacgctgat
     3121 ggccaggcat ccaagcctga tcaggagacc ggtaatcgac accggtacgg ccttgctggt
     3181 cggattcgac ccggaccgtt atgccgaaga actgttggga aaaggaccat gaccgggacc
     3241 atgaccgacg attgcctgca tcgtttcctg ttcgacgatc tggacattcg cggcgcactg
     3301 gtgcgcctcg gtccggcgtg gagggcgatg atcgagaacc gtgcctaccc tgcaccggtt
     3361 gcccagctgc tgggtgaaat gagcgccacg accgtgctcc tgggtggcca gctcaagcag
     3421 cccgggcgcc tgac
//
LOCUS       DAOSIT010000222         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24048_length_3430_cov_7.412444, whole genome shotgun sequence.
ACCESSION   DAOSIT010000222 DAOSIT010000000
VERSION     DAOSIT010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24048_length_3430_cov_7.412444"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..2858
                     /locus_tag="PLL14_06415"
     CDS             12..2858
                     /locus_tag="PLL14_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG03594.1"
                     /translation="MTPHQFIAKWRDNPLSERAGAQAFFLDLCALLGVAAPNDPDHYC
                     FERGATRTGAGQGWADVWKRGHFGWENKGPGGALGGALKQLMTYALALDNPPLLVVSN
                     RDVTQIHTHFTGTPSETHTIRLEDIGTPDNLQKLRWLFTDPERFRPGRTVQDITADAA
                     GRFAAIAQSMTGRGHDPQQVAHFLIQCLFCMFAEDIELLPKQLFERVMAKRGAETGKL
                     MAALGELFRAMQSGGDFLLEDIAWFNGGLFARVDLIELTPGEVAALHAASKMDWSAIE
                     PSILGTLFERGLDPKIRAPLGANYTDPGTIMKLVNPVIVEPLAREWAQARARIGPLVA
                     KYHAGGRGSQSAWKEAQALFLGYIERLKNFRVLDPACGSGNFLYLSLRALKDLEHRAN
                     LDAEALGLPRQLAIETSPANVLGIEINAYAAELARVTVWVGEIQWMLKNGYDFRRNPI
                     LAPLDHIEHRDALLNADGSEAAWPRADVIVGNPPFLGDKMMRGELGDDYVESLRRRYA
                     GRVPGGADLVTYWFEKSRAQIAAGGCERAGLVATNSIRQKRNRVVLERILQSTHIFEA
                     WSDEDWVNEGAAVRVSLVCFGQAGIMRLDGNKVALIHADLTGSAEDSPALDLTQARPL
                     AANKESSYFGICLAGPFKVDTTTALAWLRDTGNPNGRPNSDVMRPIYNGSDITRRWAG
                     NWVVDFAARDQADAADYLAPFAYVEAYVKPKRITNNRAARAEKWWQHGEKRPAMRAAL
                     NGLTCYIVTPETAKHRFFVRFPVQVAPEHKLIVIPRQDDVTLGVLSSRIHCLWAIEKG
                     GRLGVGNDPVYNSSLCFETFPFPLGLDLKRTVPPTGGVFQVIAQAAADLDAWREKWLN
                     PEGWLEWEITPEEQSAGLPPRPVPRPEHAREWKLRTLTNLYNDMPAGLKLRQQRLDDA
                     VAAAYGWSDYTPAMPDEEILRRLLALNRQRSAAG"
BASE COUNT          637 a         1168 c         1081 g          544 t
ORIGIN      
        1 cagccctgcc catgaccccg caccagttca tcgccaaatg gcgcgacaac ccgctgtccg
       61 agcgcgccgg cgcgcaagcc ttcttcctcg acctgtgcgc cttgctcggc gtcgcggcgc
      121 cgaacgatcc cgatcactat tgcttcgaac gcggtgcaac gcgcaccggc gccgggcagg
      181 gctgggccga tgtctggaag cgtgggcatt tcggctggga gaacaagggt ccgggcggcg
      241 ccctcggcgg cgccctcaag caactgatga cctacgcgct ggcgcttgac aacccaccgc
      301 tgctcgtcgt cagcaaccgc gacgtcaccc agatccacac ccatttcacc ggcacgccgt
      361 cggagacgca caccatccgc ctcgaagaca tcggcacgcc cgacaacctg cagaagctgc
      421 gctggctgtt caccgatccc gagcggttcc ggccgggtcg cacggtgcag gacatcaccg
      481 ccgacgccgc cggccgcttc gcggccatcg cccagtcgat gaccggtcgc ggccacgacc
      541 cgcagcaggt cgcgcacttc ctgatccagt gcctgttctg catgttcgcc gaagacatcg
      601 agctgctgcc gaagcagctc ttcgagcggg tgatggccaa gcgcggcgcc gaaaccggca
      661 agctcatggc cgcgctcggc gaactgttcc gcgccatgca gagcggcggc gacttcctgc
      721 tcgaagacat cgcctggttc aacggtggac tctttgcccg cgtcgacctg attgagctga
      781 cgcccggcga ggtcgctgcg ctgcacgccg ccagcaagat ggactggagc gccatcgagc
      841 cgtcgatcct cggcacgctc ttcgaacgcg gcctcgaccc gaaaatccgc gccccgctcg
      901 gcgccaacta caccgacccg gggacgatca tgaagctcgt caatccggtc atcgtcgaac
      961 cgctggcgcg cgaatgggcg caggcgcgcg cacgcatcgg cccgctggtc gcgaaatatc
     1021 acgccggcgg caggggctcg cagagcgcct ggaaggaagc gcaggcgctg ttcctcggct
     1081 acatcgaacg cctgaaaaac ttccgcgtcc tcgaccccgc ctgcggctcg ggcaacttcc
     1141 tgtacctcag cctgcgcgcg ctcaaggatc tcgaacaccg cgccaacctc gacgccgaag
     1201 cccttggcct gccacggcaa ctggccatcg aaaccagccc ggccaacgtg ctcggcatcg
     1261 agatcaatgc ctacgccgcc gaactggcgc gggtgacggt gtgggtcggc gaaatccagt
     1321 ggatgctgaa aaacggctac gacttccggc gcaacccgat cctggcgccg ctcgaccaca
     1381 tcgagcaccg cgacgcgctg ctcaacgccg acggcagcga agccgcgtgg ccgagggccg
     1441 atgtcatcgt cggcaatccg ccgttcctcg gcgacaagat gatgcgcggc gaactcggtg
     1501 acgactatgt tgaaagtctg cgccggcgct acgcaggccg cgttcctggc ggcgccgacc
     1561 tggtgacgta ctggttcgaa aaaagtcgcg cgcagatcgc ggccggcggc tgcgaacggg
     1621 ccgggctcgt cgccaccaac tcgattcgcc agaagcgcaa ccgcgtggtc ctggagcgca
     1681 ttctgcagag tacgcacata ttcgaagcat ggtcggatga ggactgggtc aacgaggggg
     1741 ctgcggtacg cgtcagtctg gtgtgtttcg gccaggcggg aatcatgcgg cttgatggca
     1801 acaaggtagc gctgatccat gccgacctga ccggtagtgc ggaggacagc ccggctctgg
     1861 accttaccca ggcgaggccg ctcgccgcca acaaggagag ctcatatttt ggcatctgcc
     1921 tcgccggccc cttcaaagtg gacaccacca ccgccctggc atggctgcgg gataccggca
     1981 acccgaacgg ccgtccgaac agcgacgtga tgaggcccat ttataacggt tccgacatta
     2041 cgcgacgctg ggcaggcaac tgggtggtgg attttgccgc aagggaccag gccgacgcag
     2101 ccgattatct tgcgcctttt gcctatgtgg aggcttacgt caagccgaag cgcatcacga
     2161 ataatcgtgc agcaagggcg gagaaatggt ggcagcacgg cgagaagcgc ccggcgatgc
     2221 gagccgcgct taacggactc acctgctaca tcgtcacgcc cgaaacggcc aagcaccgat
     2281 tcttcgtcag gttcccggtg caggttgccc ctgagcacaa gctgatagtc attccgcgcc
     2341 aggatgatgt aacgttgggt gtcctgtcgt cacgcatcca ctgcctgtgg gctatcgaga
     2401 aaggcgggcg tttaggcgtt ggcaatgatc cggtttacaa ctccagcctg tgctttgaaa
     2461 cctttccgtt tccgctcggc cttgacctca agcgtacggt tccgccaact ggtggtgtct
     2521 ttcaggtcat tgcccaggcc gctgccgacc ttgatgcctg gcgggaaaaa tggctcaacc
     2581 ccgagggctg gctggagtgg gaaatcacac cggaagaaca aagcgcgggt cttcccccgc
     2641 gccccgtccc acgtccggaa cacgctcggg aatggaagct acgcacgtta acaaacctat
     2701 acaacgatat gcctgccggg ctcaaactgc gccagcaaag actcgacgac gccgtcgccg
     2761 ccgcctatgg ctggagcgac tacactccgg cgatgcccga cgaggaaatc ctgcgccggc
     2821 tgctggccct gaaccggcag cgttcggccg ccggctgaag cgccggtacc cggcacctcg
     2881 gcaagggcca tgggccgctg gtgttcgcgg cacgcgacga cgaatggaca tctcgacagg
     2941 attcccggcg taaaagccag gtcggcgtcc gcccgctggt ggtggccaga tgtccgggta
     3001 acgggtagac tgcgcggcgg cgtgatgatc tccgccggcg gatctcggcc gcgacagcga
     3061 ggtcaggcaa ctcgaaaatt ctgaaggtca gagatgaatc cttgtcgctg caaactattc
     3121 cggcaggccc gcggcgggcg cccgccgacg agagtcgcgc acaaccggca agcgcgcgcg
     3181 gccatgccgc ggcgccatcc cgccgcgccg ggcgactgac gcgaagaacc gatcggcaag
     3241 ccgcgcgtgt ccatgccagg gaacccccgc cgccgtacca ccttcgtgtt gatcgcggtg
     3301 ctggtggcgc tcggggcgtt cgccaccacc gcctatgtgg tgtggcggtt gcgggcgaaa
     3361 accgtcgacc agcaactcgc cacggcggcc ctcaccgcgc gtgcgctgga agaccacctg
     3421 acccagagct
//
LOCUS       DAOSIT010000223         3429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24065_length_3429_cov_11.353883, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000223 DAOSIT010000000
VERSION     DAOSIT010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24065_length_3429_cov_11.353883"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PLL14_06420"
     CDS             complement(<1..134)
                     /locus_tag="PLL14_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOG03595.1"
                     /translation="MSQFDNVSVVKQANVYFDGKCISHTIRLADGSSKTVGVILPSTL
                     T"
     gene            complement(138..347)
                     /locus_tag="PLL14_06425"
     CDS             complement(138..347)
                     /locus_tag="PLL14_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOG03596.1"
                     /translation="MFGLTEEQISDFGMTFGVGAFMLFMLFIIGEIAWKAKAGKTGTI
                     ILFFVLSFGMVGFIAKTIMEKLWGL"
     gene            complement(366..1595)
                     /locus_tag="PLL14_06430"
     CDS             complement(366..1595)
                     /locus_tag="PLL14_06430"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG03597.1"
                     /translation="MSDIKKAVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEE
                     LDAARPKALQFGIAPENIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRMIEIANLTGADAVAHGATGKGNDQVRFELGAYALRPDIRIIAPWREWDLLSREKLL
                     AYAELHAIPVEMKHRQGGSPYSMDANLLHISYEGRHLEDPAAEAEESMWRWTVSPEMA
                     PDAPEYVDLEFAHGDLVSINGLHMPAHELLALLNRLGGKHGIGRIDLVENRYVGMKSR
                     GCYETPGGTILLPAHRAIESITLDREVAHLKDDLMPRYASLIYNGYWWSPERRALQTL
                     IDHTQQAVNGWVRVKLYKGNVKVVGRDSKSDSLFDSTIATFEDDAGAYDQKDAAGFIK
                     LNALRMRIAANLASRKS"
     gene            complement(1592..2566)
                     /gene="argF"
                     /locus_tag="PLL14_06435"
     CDS             complement(1592..2566)
                     /gene="argF"
                     /locus_tag="PLL14_06435"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG03598.1"
                     /translation="MDISPRHFLQFKDFSREEFDHLFQRALWIKSEFKAYRKYWPLID
                     RTLVMIFEKASTRTRLSFEAGMQQLGGLAIYLNSDDSQIKRGEPIEDTAQVISRMSDA
                     VMIRTFGHDRIERFAACSRVPVINGLTNEYHPCQILADVFTFIEHRGPIQGKTVAWIG
                     DSNNVCNTWLQAAEILDFKLHVSTPPGYEVAPERAGLRGTAHLEQFADPMDAAHLADL
                     VTTDVWTSMGFEAENDRRRRDFAAWQVNPEMMRVAHPEALFMHCLPVHRGEEVAAEVI
                     DGPQSVVWDEAENRMHTQKALLEFLLLGRVDSAAAPSPSASTPSRELP"
     gene            complement(2578..>3429)
                     /locus_tag="PLL14_06440"
     CDS             complement(2578..>3429)
                     /locus_tag="PLL14_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03599.1"
                     /translation="YGHQQGIDSPATIVMEKAFHGRTMATLSATGNRKAQAGFEPLVS
                     GFVRVPYNNLEAIRTLARYNKNVVAVMLEIVQGEGGINIADIDFQRGLRALCDDRGWL
                     LICDEVQCGMGRTGTWFAFQHAGIRPDVVTLAKGLGGGVPIGACLAAGQAKGLFKPGN
                     HGSTFGGNPLATTAALTTIGVIENDRLIEHARIVGASIREGLALALAGCAGIVEIRGQ
                     GLMIGIELDRECGELVTRGLTAGLLINVTAEKVVRLLPALTLGADEAQELVSRLARLL
                     RDFLAVP"
BASE COUNT          632 a         1101 c         1049 g          647 t
ORIGIN      
        1 gtcagcgtcg atggcaggat cacgccgacc gtcttgctgc tgccatccgc caaccggatc
       61 gtgtggctga tacatttgcc gtcgaagtag acgttggcct gcttgacgac gctgacattg
      121 tcaaactgcg acatgattca taatccccag agtttttcca tgatggtctt ggcgatgaaa
      181 ccgaccatgc cgaaggatag tacgaagaac aatatgatgg tgccggtctt cccggccttc
      241 gctttccagg caatctcgcc gatgatgaag agcatgaaga gcatgaacgc gccgaccccg
      301 aaggtcatgc caaaatccga aatctgctct tccgtcagtc cgaacatcga cggcgcgctc
      361 cccgatcagc tcttgcggct cgccagattg gccgcaatgc gcatgcgcag cgcattgagc
      421 ttgatgaaac cggcggcgtc cttctggtcg tatgccccgg catcgtcctc gaaggtggca
      481 atcgtcgagt cgaacagcga atcggatttc gaatcacggc ccaccacctt gacgttaccc
      541 ttgtaaagct tgacccgcac ccagccattc accgcctgct gcgtatggtc gatcagggtc
      601 tgcagggcgc gccgctccgg gctccaccag tatccgttgt agatcaggct ggcatagcgc
      661 ggcatcaggt cgtccttgag atgcgccact tcgcggtcga gggtgatcga ctcgatcgcc
      721 cggtgcgccg gaagaaggat cgtcccgccg ggagtctcgt agcagccgcg cgacttcatg
      781 cccacgtaac ggttctcgac cagatcgatg cgcccgatgc cgtgcttgcc gccgaggcgg
      841 ttgagcagcg ccagcagttc gtgcgccggc atgtgcaggc cattgatcga caccaggtca
      901 ccgtgcgcga attcgaggtc aacgtactcg ggggcgtccg gcgccatttc cggcgacacc
      961 gtccagcgcc acatcgactc ctcggcctcg gctgccgggt cctcgaggtg acggccctcg
     1021 tagctgatgt ggagaaggtt ggcgtccatc gaatacggcg agcccccctg ccgatgcttc
     1081 atctcgaccg ggatggcgtg cagctcggca taggccagca gcttctcgcg cgacagcaga
     1141 tcccactccc gccagggggc gatgatcctg atgtcgggcc tgagcgcgta ggcgccgagt
     1201 tcgaaacgaa cctgatcgtt gcccttgccg gtggcaccgt gcgccaccgc atcggcgccg
     1261 gtcaggttgg cgatctcgat catccgcttg gcgatcagcg gccgggcgat cgaggtcccg
     1321 agcagatact cgccctcata caccgtattc gcccggaaca tcgggaagac gaagtcgcgc
     1381 acgaactcct cgcgcaggtc gtcgatgtag atgttttccg gcgcgatacc gaactgcagg
     1441 gccttggggc gcgccgcgtc gagttcctca ccctggccga ggtcggcggt gaaggtcacg
     1501 acttcgcact gataggtatc ctgcaaccat ttcaggatca cggaagtatc gaggccgccc
     1561 gaataggcga ggaccgcttt cttgatgtcg ctcatggtaa ttcccggctt ggagtggacg
     1621 cggaaggaga gggtgcagcg gcggagtcga cgcgcccgag aagcagaaat tcgagtagcg
     1681 ccttctgggt atgcatgcgg ttctcggctt cgtcccagac gacgctctgc ggaccatcga
     1741 tgacctcggc ggcgacttcc tcgccgcgat ggaccggcag acaatgcatg aacagtgcct
     1801 cgggatgggc gacgcgcatc atttccgggt tgacctgcca ggcggcgaaa tcgcgcctgc
     1861 gccggtcgtt ctcggcttcg aaacccatcg aggtccacac gtcggtggtc accagatcgg
     1921 ccaggtgcgc cgcgtccatc gggtcggcga actgttcgag gtgcgcggtg ccgcgcaggc
     1981 cggcccgttc aggggccacc tcgtagcccg gcggggtcga cacgtgcaac ttgaaatcaa
     2041 gtatttccgc cgcctgcagc caggtattgc agacattgtt cgagtcgccg atccaggcca
     2101 ccgtcttgcc ctggatcgga ccccggtgct cgatgaaggt gaacacatcg gccagaatct
     2161 gacagggatg gtactcgttg gtcaggccat tgatcaccgg cacgcgcgag caggccgcga
     2221 aacgctcgat ccgatcatgc ccgaaagtgc ggatcatcac cgcgtcgctc atgcgcgaga
     2281 tcacctgtgc cgtatcctcg atcggctcac cgcgcttgat ctgcgaatcg tcgctgttga
     2341 gatagatcgc caggccacca agctgctgca tgcctgcttc gaacgagagg cgggtgcgcg
     2401 tgctggcctt ctcgaagatc atcaccagcg tacgatcgat cagcggccag tatttgcggt
     2461 aagccttgaa ttccgacttg atccagagcg cacgctggaa caggtggtcg aactcctcgc
     2521 ggctgaaatc cttgaactgc aggaagtgtc ttgggctgat gtccatggcc gttctcttta
     2581 ggggactgcg agaaaatcgc ggagcagtcg cgccagtcgc gacaccagct cctgcgcttc
     2641 gtcggcgccg agggtcagcg ccggcagcag gcgcacgacc ttctcggccg tgacattgat
     2701 cagcagccca gcggtgaggc cgcgcgtcac cagttcgccg cactcgcgat cgagctcgat
     2761 gccaatcatc agtccctgac cgcgaatttc gacaataccc gcgcaacccg ccagcgccag
     2821 cgccagcccc tcgcggatcg acgccccgac gatccgcgca tgctcgatca ggcgatcgtt
     2881 ttcgatcacc ccgatggtcg tcaaggcggc ggttgtcgcc agaggattgc cgccgaaggt
     2941 cgagccgtga ttgccgggct tgaacagccc tttcgcctgg ccggcggcga ggcaggcacc
     3001 gatcggcacg ccaccaccca gtcccttggc cagcgtcacc acgtccgggc ggatacccgc
     3061 gtgctggaat gcaaaccagg taccggtgcg gcccatgccg cactgcacct catcgcagat
     3121 cagcagccag ccgcggtcgt cacacagcgc acgcagaccg cgctgaaaat cgatgtccgc
     3181 gatattgatg ccaccctcgc cctgcacgat ttcgagcatc accgcgacga cgttcttgtt
     3241 gtacctggcc agcgttctga tggcttcgag gttattgtag ggaacacgca cgaagcccga
     3301 caccagcggt tcgaaaccgg cctgcgcctt gcgattgccg gtggccgaca gggtcgccat
     3361 ggtgcggccg tgaaacgcct tctccatgac gatggttgcc gggctatcga tcccctgctg
     3421 gtggccgta
//
LOCUS       DAOSIT010000224         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24116_length_3423_cov_10.269893, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000224 DAOSIT010000000
VERSION     DAOSIT010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24116_length_3423_cov_10.269893"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PLL14_06445"
     CDS             complement(<1..1121)
                     /locus_tag="PLL14_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HOG03600.1"
                     /translation="MATLFFVLGIPLLGSVVLALSGHRDAGREINVAFSLGTFVATCV
                     LTTEVIAGGPLFAWNREFYIDPLNVFLVTLTAFVGLTTAIFSRPYMRVERDHGKMTPN
                     RMRLYHSMYQLFSFTMLIALTTNNLGILWVAMEAATLTTVLLVSVYRTPASLEAAWKY
                     FILCGVGIAQALFGTVLLYMAAEKVIGTEGGALLWTNLDAVKAQLDPAIITLAFAFLF
                     IGYGTKVGLVPMHNWLPDAHAEGPTPVSAVLSGLLLNVAVYALLRCKVLTDGALHSPL
                     AGQLMIAFGLLSVLVAAFFLSRQKDVKRMFAYSSIEHMGLITFAFGIGGPIANYAGLL
                     HMTVHSLIKSGIFFAVGHATQKAGTQNMADIRGLIKVSPT"
     gene            complement(1123..1782)
                     /locus_tag="PLL14_06450"
     CDS             complement(1123..1782)
                     /locus_tag="PLL14_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HOG03601.1"
                     /translation="MTELVGQLINLLAAILLMLSFAMISQRRILSLIHLFTAQGATLV
                     AATLLVGYATQQPHLYLSAGITLLLKVIVIPLLLHRVITRLDVRWDVETLINIPMTMI
                     IGIALVIFAFNLALPISKLSVSLARGTLGIALACVLLSFMMMITRSKAVPQVIGFLSM
                     ENGLFFAATSATYGMPMVVELGIALDVLVGILILGVFLFHIREQFDSLDISHLEKLKE
                     D"
     gene            complement(1784..2731)
                     /locus_tag="PLL14_06455"
     CDS             complement(1784..2731)
                     /locus_tag="PLL14_06455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOG03602.1"
                     /translation="MTGLGAFSQLLEIVMALLLAPLLGGWINQWRAWLQNRSAPGLLQ
                     PYRMLHKLFNKESVVAEHASPLFRGAPYIVFGCMALACAIIPTLSTDLPLSPAADAIA
                     LVGLFALARVFISLAAMDVGTSFGTLGARREMLIGFLAEPALLMVLFSASLISKSTSL
                     TTIVETLAHRDLAIYPSLALAGVAFTMVSLAENARVPVDNPATHLELTMIHEALILEY
                     SGRHLALLEWAASLKLFAYSCIGLALFFPWGIAEAEAPLALLLAMPVLILKLAIGGAL
                     LALLETLSAKMRIFRVPEFLATAFLLAVIGMLVNILLGG"
     gene            complement(2728..>3423)
                     /locus_tag="PLL14_06460"
     CDS             complement(2728..>3423)
                     /locus_tag="PLL14_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HOG03603.1"
                     /translation="ALIAALAGYTMVKFFGVIFLGQPREERLAQAHDASGWERLGMLW
                     LAAGCLVLGLCPVQFIAVIDPVTQLLVGAGMGEAIAAGNWLLVPVAIERASYGPAIFL
                     LGVAASFALAFLLVRLLYHGRLRRAAPWDCGYPWQNARMQDTAEGFGQPIRQIFEPFF
                     RMQRELPSPFDAAPHYRVSVGDHFWHWLYLPLAALVERLARLIGLLQQGRIAVYLLYS
                     FVTLMIVLAVVKQ"
BASE COUNT          720 a         1068 c         1136 g          499 t
ORIGIN      
        1 gtcgggctga ccttgatcag gccgcggatg tcggccatgt tctgcgtccc ggccttctgc
       61 gtcgcatggc cgacggcaaa gaagataccc gacttgatca gcgaatggac cgtcatgtgc
      121 aagaggccgg catagttggc aatcgggccg ccgatgccga aggcgaaggt gatcagcccc
      181 atgtgttcga tcgacgaata ggcgaacatg cgcttgacat ccttctgccg cgacaggaag
      241 aaggccgcca cgagcaccga cagcaggccg aaggcgatca tcaactgacc ggcgagtggg
      301 ctgtgcagcg cgccgtcggt gagcaccttg cagcgcagca gggcatacac cgcgacgttg
      361 agcagcaagc ccgagagcac cgccgaaacc ggcgtcggtc cctcggcgtg cgcgtccggc
      421 aaccagttgt gcatcggcac caggccgacc ttggtgccgt agccgatgaa caggaaggca
      481 aacgcgaggg tgatgatggc cgggtcgagt tgcgccttga cggcgtcgag attggtccat
      541 agcagcgcgc ccccctcggt gccgatgacc ttttccgccg ccatgtacag cagcacggtc
      601 ccgaacagcg cctgcgcgat gccgacgccg cagaggatga agtatttcca ggccgcctcc
      661 aggctcgccg gtgtgcggta gacgctgacc agcagaacgg tggtcagcgt cgccgcctcc
      721 atcgccaccc acaggatccc cagattgttg gtcgtcagcg cgatcagcat ggtgaagctg
      781 aacaattgat acatgctgtg gtagaggcgc atgcgattcg gcgtcatctt gccgtgatcg
      841 cgttcgacac gcatgtaggg gcgcgagaag atggccgtgg tgaggccgac gaaggcggtc
      901 agggtgacca ggaagacgtt cagcggatcg atgtagaact cgcgattcca ggcgaagagc
      961 ggaccgccgg cgatgacctc ggtagtgagc acgcaagtgg cgacgaaggt ccccaggctg
     1021 aaggcgacat tgatctcgcg tccggcgtcg cggtgcccgc tcagggcgag aacgacgctg
     1081 ccgagcaagg ggatgccgag aacgaaaaac agcgtcgcca attcagtctt ccttgagctt
     1141 ttcgaggtgg ctgatgtcca ggctgtcgaa ctgttcacgg atatggaaca ggaacacgcc
     1201 gaggatcagg atcccgacca ggacgtcgag ggcgatgccg agttcgacga ccatcggcat
     1261 gccgtaggtg gccgaggtgg cggcgaagaa cagcccgttc tccatcgaca ggaagccgat
     1321 tacctgcggc accgctttcg agcgggtgat catcatcatg aacgagagca gcacgcaggc
     1381 cagtgcgatg ccgagcgtgc cgcgggccag gctgaccgac agcttggaga tcggcagcgc
     1441 caggttgaag gcaaagatca ccagcgcgat gccgatgatc atcgtcatcg ggatattgat
     1501 cagcgtctcg acgtcccagc gcacatcgag gcgggtgatg acgcgatgca gcagcagcgg
     1561 gatgacgatc accttgagca gcagcgtgat accggccgag aggtacaggt gcggttgctg
     1621 cgtggcgtag ccgaccagca gcgtcgccgc caccagggtc gccccctgtg cggtaaacag
     1681 gtgaatcagc gacaggattc gccgctgcga gatcatcgcg aacgacagca tcaggaggat
     1741 cgccgccagg agattgatca gttgtccgac gagttcggtc atctcacccg cccagcagga
     1801 tgttgaccag catgccgatc accgccagca gaaatgcggt ggccagaaac tccggcacgc
     1861 ggaagatgcg catcttggca ctgagggttt cgagcagggc gagcagcgcg ccaccgatcg
     1921 ccagcttcag gatcagcacc ggcatcgcca gcagtagcgc cagcggcgcc tcggcctcgg
     1981 caatgcccca ggggaagaac aacgccagac cgatgcacga gtaggcgaag agtttcaggc
     2041 tcgcggccca ttcgagcaga gccaggtgac gtcccgagta ttcgaggatc agcgcctcgt
     2101 gaatcatcgt cagttcgaga tgggtcgccg ggttgtccac cggcacgcgc gcgttttcgg
     2161 ccagggagac catggtgaac gccacgccgg cgagcgccag actcggatag atggccaggt
     2221 cgcgatgcgc cagggtttcg acgatggtcg tcagcgaggt cgacttcgag atcagcgaag
     2281 ccgaaaacag caccatcagc aacgccggct cggccagaaa accgatcagc atctcgcgcc
     2341 gggcgccgag cgtgccgaaa gaggtgccga catccatcgc cgccagcgaa atgaacaccc
     2401 gcgccagggc gaacaggccg accagcgcaa tcgcatcggc cgccggcgac agcggcagat
     2461 ccgttgacag cgtcggaatg atcgcgcagg ccagcgccat gcaaccgaag acgatgtacg
     2521 gtgcgccgcg aaacagtggc gaggcgtgct cggcgaccac cgattccttg ttgaacagct
     2581 tgtgcagcat ccggtagggc tgcagcaggc ccggcgccga gcggttctgc agccaggcgc
     2641 gccactggtt gatccagccg ccgagcaagg gcgccagcaa cagcgccatg acgatttcca
     2701 gcagttgcga gaaagcaccg aggccggtca ttgtttgacc accgccagca cgatcatcag
     2761 ggtgacgaag ctgtagagca ggtacaccgc gatccgcccc tgctgcagca gtccgatcag
     2821 ccgcgccagt cgctcgacga gggcggccag cggcaggtac agccagtgcc agaaatggtc
     2881 gccgacgctg acgcgatagt gcggggccgc gtcgaacgga ctcggcagtt cccgctgcat
     2941 gcggaagaag ggttcgaaga tctggcgtat gggttgaccg aaaccctcgg cggtatcctg
     3001 catgcgcgcg ttctgccacg gatatccgca atcccagggc gccgcgcggc gcagacggcc
     3061 gtggtacagc aggcgcacca gcaggaaagc cagcgcgaag ctcgccgcga ccccgagcag
     3121 gaagatcgcc ggcccgtaac tcgcgcgctc gatggccacc ggcaccagca gccagttgcc
     3181 ggcagcgatc gcctcgccca tgccggcgcc gaccagcaat tgcgtcaccg gatcgatgac
     3241 ggcaatgaac tgtaccggac acagtcccag caccaggcag cccgcggcca gccagagcat
     3301 gcccagccgc tcccagccgc tggcgtcatg cgcctgcgcc agccgctcct cgcgcggctg
     3361 cccgaggaag atgacgccga agaacttgac catggtatag ccggcgagcg cggcgatcag
     3421 cgc
//
LOCUS       DAOSIT010000225         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24117_length_3423_cov_7.522268, whole genome shotgun sequence.
ACCESSION   DAOSIT010000225 DAOSIT010000000
VERSION     DAOSIT010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24117_length_3423_cov_7.522268"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLL14_06465"
     CDS             complement(<1..619)
                     /locus_tag="PLL14_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG03604.1"
                     /translation="MADRRLQVFHAVARHLSFTKAGEALHMTQPAVTFQIKQLEERFN
                     ARLFDRGGGRISLTPAGELVLDYAERILGLSNELDIRVAEMTGEMSGSLLVGASTTIA
                     EFMLPPILGEFNVRHPQVRARLTVAHSEAIGNAIAAHTLDIGLIESHANHPSLQCEIC
                     GDDELQVVCTPNHPLARRGEVDARGLLTHDFIAREPGSGTREVTDD"
     gene            932..2194
                     /gene="lplT"
                     /locus_tag="PLL14_06470"
     CDS             932..2194
                     /gene="lplT"
                     /locus_tag="PLL14_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOG03605.1"
                     /translation="MPFGFYIIMAAQFFSALADNALLIVAISILREMHAPSEYEPLLK
                     TFFTVSYVALAAFVGAFADSMPKWRVMFISNGIKIVGCAMMYWDVHPLLAYAVVGLGA
                     AAYSPAKYGILTEYLPHRLLVVANGWIEGLTVAAIILGVVVGGTLIQPAVANSLLTMD
                     FPVIDTGVDTIGEMSLCFVAMFYLLAALFNIYIPDTGVDHRVLRKNPWYLVREFNHCL
                     ALLWRDRLGQVSLAVTTLFWGAGATLQFIVIKWAESALQLGLSKSSMLQGVVAVGVAL
                     GAAGAARMITLRKSVRVIPLGIAMGIIVLVMNFVHHLWLAVPLLVLIGALSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILALTGFYFLMIRADLSIYLVITLFGVFVS
                     GAMWLVKGRHEANQEIHDDVSQLDDSTH"
     gene            2321..2842
                     /locus_tag="PLL14_06475"
     CDS             2321..2842
                     /locus_tag="PLL14_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG03606.1"
                     /translation="MSKITIDRYDLALLDALQRKGNATNATLGEQIRLSASQISRRIQ
                     RLEEDGVIAGYVALLAPSALGLGITAFAQVILERHGEAASEAFENAVAAMPEVTDCLS
                     VSGDADYMLRIVVPDLAEFSELMMKRLLRLPGVARIKTSIALQTIKQTHVLPLDHLTQ
                     PNKPRQRVRYAEG"
     gene            complement(2839..3336)
                     /locus_tag="PLL14_06480"
     CDS             complement(2839..3336)
                     /locus_tag="PLL14_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG03607.1"
                     /translation="MDLRRLFRHLLLPHWWVLRVFPPRLLGKIEQAVKSSESGHSGEL
                     RVVVEANLPLAALWHAQSVRARAVELFAQTRVWDTEHNSGVLIYLQLIDRRVEIIADR
                     GINAKVGQPFWERVCRDIEAAFRTGDFEGGTLQALRTITEALAEHFAASAENSNELPD
                     APLLL"
BASE COUNT          634 a         1065 c         1012 g          712 t
ORIGIN      
        1 agtcgtcggt gacttcgcgg gtaccggagc ctggctctcg ggcaatgaag tcgtgtgtca
       61 gcaggccgcg cgcatcgact tcgccacgcc tggccagcgg atgattgggc gtgcacacga
      121 cctgcagttc atcgtcaccg cagatctcac actgcaggct cgggtgattg gcgtgcgact
      181 cgatcagccc gatgtcgagc gtgtgtgcag cgatggcgtt accgatcgct tccgaatgcg
      241 cgacggtcaa gcgtgcccga acctgcgggt gacgcacatt gaattcgccg aggatcggtg
      301 gcagcatgaa ctccgcaatc gtggtgctcg cgccgaccag cagactgccg ctcatctccc
      361 cggtcatttc ggcgacgcgg atgtcgagtt cgttggatag tccgaggata cgttcagcgt
      421 agtcgagcac cagttcaccg gccggcgtca gcgagattcg tccgcctccc cggtcaaaaa
      481 ggcgtgcatt gaagcgctct tcgagttgtt tgatctggaa ggtcacggcc ggctgagtca
      541 tgtgcagcgc ctcgccggct ttggtgaacg aaaggtgcct ggccaccgca tgaaacacct
      601 gtaaacgacg atcagccatc agtcatcccc ttgtttcgac ttatgcgcag attcaaacac
      661 aaatccgcgt cgccggccag agagcagata gccatgttgg tccacccgaa tagcgcatcc
      721 cgaaaccctt cccctggccc gcaaacctcg gacgctgcaa ggcgtaggct ccgaatgccc
      781 ctggcagatc tgcatcgacg ccgccgggaa cgtttccgtc ggatgcccgg cccggcgagt
      841 cgtgcgtgcg gcaggcccgg agcgctaaca agttgccggc tttcatggta taaggcctgc
      901 acttcgagaa cgggcgcatt catcacatcc aatgcctttc ggcttctaca tcatcatggc
      961 ggcgcagttt ttttcggcgc tggccgacaa tgccctgctc atcgttgcca tctcgatcct
     1021 gcgcgaaatg cacgcgccca gcgaatatga gccgctgctc aagaccttct tcacggtttc
     1081 ctatgtcgcg ctggcggcct tcgtcggcgc tttcgccgac tcgatgccca agtggcgggt
     1141 gatgttcatc agcaatggca tcaagattgt cggttgcgcg atgatgtact gggatgttca
     1201 cccgctgctc gcctatgcgg tggtcggtct cggtgcagct gcctactcac cggccaagta
     1261 tgggattctg accgagtacc tgccccaccg cctgctggtg gtcgccaatg gctggatcga
     1321 gggactgacg gtggcagcga tcattctcgg cgtcgtcgtt ggtggtactt tgatccagcc
     1381 cgcggtggcc aacagcctgc tgaccatgga ctttcctgtc atagacaccg gagtcgacac
     1441 catcggtgag atgtccctgt gctttgttgc catgttctac ctgttggccg cgctgttcaa
     1501 catctacatt ccagacactg gcgtcgatca ccgggtcctg cgcaagaatc cgtggtatct
     1561 ggtgcgcgag ttcaatcatt gcctggcact gctgtggcgg gaccgcctgg gtcaggtttc
     1621 gctggcggtc acgacgctgt tctggggggc cggcgcgacg ctgcagttca ttgtgatcaa
     1681 atgggctgaa agcgctctgc agctcggttt gtcgaagtcg agcatgctgc agggcgtggt
     1741 cgccgtcggg gttgccctgg gggcagccgg cgcggcgaga atgatcacct tgcgcaaatc
     1801 ggtgcgcgtg attccgctcg ggatcgccat ggggatcatt gtgttggtga tgaactttgt
     1861 gcatcacctg tggcttgccg tgccgctgct ggtcctgatc ggcgccctct ccggtttctt
     1921 cgtcgtgccg atgaacgctc tgctgcagca ccggggccac atcctgatgg gggccggaca
     1981 ttcgattgct gtgcagaact tcaacgaaaa cctgtcaatc cttgccctga ccggtttcta
     2041 ctttctgatg atccgcgctg acttgtcgat ctatctggtg atcaccctgt tcggcgtctt
     2101 tgtcagcggc gcgatgtggc tggtcaaagg ccgccatgaa gccaatcagg aaatccacga
     2161 cgacgtcagt caactcgacg acagcacaca ctgaactgtg cccattcgcg gccgggcatt
     2221 gagccgtttc acgccagcca cgaaacgttt cagggtggtg tttcacatgg cggcataaac
     2281 ccctatactg cgcacactgc atgcaccaat atggcgaata atgtccaaaa tcaccatcga
     2341 tcgttatgat cttgcactgc tcgacgccct gcagcgcaag ggcaacgcca ccaacgccac
     2401 gctcggcgaa cagatccgtc tgtcggcgtc acagatcagc agacgtatcc agcgtctgga
     2461 ggaagacggg gtcatcgccg gctatgtggc gttgctcgcc ccctcggcgc tgggcctggg
     2521 gatcaccgct tttgcgcagg tgatcctgga gcggcacggg gaagccgcca gtgaagcctt
     2581 tgaaaacgcg gtggcggcaa tgccggaagt aacggactgc ctttcggtaa gtggtgacgc
     2641 cgactacatg ctgcgcatcg tcgttcccga tctggccgaa ttttcggaac tgatgatgaa
     2701 acgcctgctg cgcctgcctg gtgtcgcccg catcaagacc agcattgccc tgcagacgat
     2761 caagcagacg cacgtcctgc cgcttgatca cctgacgcag ccgaacaagc cgcgtcagcg
     2821 cgtgcgctat gccgaaggct agagcagcaa cggtgcatcc ggcagctcgt tggagttttc
     2881 agcagacgcg gcgaagtgtt cagccaatgc ctcggtgatc gttcgcagcg cctgcagcgt
     2941 accaccctcg aaatcaccag tgcggaaggc ggcttctatg tcccggcata cgcgctccca
     3001 gaatggttgg ccgaccttgg cgttgattcc ccggtcggca ataatttcga cacgccggtc
     3061 gatcagttgc aggtaaatca gtacgccgct gttgtgctcg gtatcccaga cacgtgtctg
     3121 cgcaaacagc tcaaccgccc gtgcgcgcac cgactgggca tgccagagcg cggccagcgg
     3181 caggttcgct tcgacaacca cgcgcagctc gccagaatgc cctgattccg aggatttgac
     3241 cgcctgctcg atcttgccga gcaggcgagg tggaaataca cgcagcaccc accagtgtgg
     3301 cagcaacaga tggcggaaca gccttctcag atccatcacc agtctcccga tgcgccgccg
     3361 ccaccaaagc tgccgccgcc gccagaaaac ccgctcccgg aaccgccccc gccgccccac
     3421 gat
//
LOCUS       DAOSIT010000226         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24283_length_3404_cov_11.294715, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000226 DAOSIT010000000
VERSION     DAOSIT010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24283_length_3404_cov_11.294715"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..2713)
                     /gene="secA"
                     /locus_tag="PLL14_06485"
     CDS             complement(2..2713)
                     /gene="secA"
                     /locus_tag="PLL14_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG03608.1"
                     /translation="MISGLLKKIFGSRNDRLIKQYSQVVRKINALEAGISALSDEGLR
                     GKTAEFKTRAANGETLDALLPEAFAVVREAGKRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLASYLNALSGNGVHVVTVNDYLANRDAEWMGRLHRFLGLTVGVNLSQ
                     MAHEDKQAAYAADITYGTNNEFGFDYLRDNMVYSGGERVQRKLSFAIVDEVDSILIDE
                     ARTPLIISGQAEDHTDLYVRMDKVAPLLKRCAEENGPGDYWVDEKGHQILMTEEGHEH
                     AEEILAANGLLPDGGSLYDASNILMIHHLYAALRAHNLFLRDQQYVVQNGEVIIVDEF
                     TGRLMSGRRWSDGLHQAVEAKEGVRIQNENQTLASITFQNYFRMYGKLSGMTGTADTE
                     AYEFQQIYGLETVVIPTNQPMQRTDNNDQVFRTAKEKHTAIVADIRGCRERGQPVLVG
                     TTSIENSELLSALLDHEKLPHQVLNAKQHAREAEIVREAGRPGVITIATNMAGRGTDI
                     VLGGSIEKQISQIRDDEALPQADRDARIAEIRSQWQKLHEQVVAAGGLHIIGSERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLALDDSLLRIFAGDRLKSIMERLKMPEGEPIEHPL
                     VSRSLESAQRKVEARNFDMRKQLLEYDDVANDQRKVIYAQRNELLETEDISETVTAMR
                     EGVLHDAFRLHVPAESVEEQWDLPGLEKTLASDLQLQLPIVEWSKDEPSLRDEAILRR
                     IVDAASTSYTAKVELVGAEVFHQFERNVMLQSLDTHWREHLAALDHLRQGIHLRGYAQ
                     KNPKQEYKREAFELFQRLLDSVRADVTRLLMTVQIRAQDVEETAPHAAVQNVRYQHAD
                     YDDALGQGNEAAAVAQKPLPTGPKVGRNDPCPCGSGKKYKFCHGKLS"
     gene            3152..>3404
                     /locus_tag="PLL14_06490"
     CDS             3152..>3404
                     /locus_tag="PLL14_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOG03609.1"
                     /translation="MPPAVEAYTVQDYLQWEGDWELIHGQPVAMVPSPSFGHQQVSAA
                     IFRQLDESLDDCPHCQAVFEIDVEFAQDTVVRPDVLVICY"
BASE COUNT          579 a         1050 c         1073 g          702 t
ORIGIN      
        1 gctacgacaa cttgccgtga cagaatttgt acttcttgcc cgagccgcag gggcaggggt
       61 cgttgcggcc gactttcggg cctgtcggca ggggcttctg agcgacagcc gccgcctcat
      121 taccctgtcc caaggcgtcg tcgtagtccg catgctggta gcggacgttc tgcacggcgg
      181 cgtgcggggc ggtttcctcg acatcctgcg cgcgtatctg aacggtcatc agcagacgcg
      241 tcacgtcggc acggacactg tcaagcagcc gttgaaacag ttcgaacgct tcgcgcttgt
      301 attcctgctt cgggttcttc tgcgcgtagc cgcgcaggtg tatcccctgc cgcaaatgat
      361 cgagggccgc caggtgttcg cgccagtgcg tgtccaggct ctgcagcatg acgttgcgct
      421 cgaactggtg aaagacctcg gcaccgacga gctcgacctt ggccgtgtac gaggtggaag
      481 ccgcgtcgac gatccgcctg aggatggctt cgtcgcgcag gctcggttcg tctttcgacc
      541 actcgacgat cggcagttgt agctgcagat cggaagcgag cgtcttttcc aggcccggca
      601 ggtcccattg ttcctcgacg ctctccgccg ggacatgcaa ccggaaggcg tcgtgcagca
      661 cgccttcgcg catcgcggtc acggtttcgg agatgtcttc ggtttccagc aactcgttgc
      721 gttgcgcata aatgaccttg cgctgatcgt tggccacgtc atcgtattcg agaagctgct
      781 tgcgcatgtc gaagttgcgg gcctcgacct tgcgctgtgc cgactccagc gaacgcgaaa
      841 ccagcgggtg ctcgatcggc tcgccttcgg gcatcttcag ccgttccatg atcgatttca
      901 ggcgatcacc ggcaaagatg cgcagcagcg agtcgtcaag tgcgagatag aagcgcgacg
      961 aacccgggtc cccctggcga cccgaacgcc cgcgcaactg gttgtcgata cgccgcgact
     1021 catgccgctc ggagccgatg atgtgcaggc cgccggcggc aaccacctgt tcgtgcagct
     1081 tctgccattg cgagcggatc tcggcgatgc gcgcatcgcg atcggcctgg ggcagtgctt
     1141 catcgtcgcg gatctgggag atctgcttct cgatgctgcc gccaagcaca atgtcggtac
     1201 cgcgaccggc catgttggtg gcaatggtga tcactcccgg ccggccggcc tcgcgcacga
     1261 tctcggcctc gcgcgcatgt tgcttggcgt tgagcacctg atgcggaagc ttttcgtggt
     1321 cgagcagcgc cgagagcaat tcggagtttt cgatcgaggt cgtgccgacc agcaccggct
     1381 gcccgcgttc acggcagcca cggatgtcgg cgacgatcgc ggtatgtttt tccttcgccg
     1441 tgcggaagac ctgatcgttg ttgtcggtgc gctgcatcgg ctggttggtc gggatgacca
     1501 ccgtttcgag gccgtagatc tgctggaatt cgtaggcttc ggtatccgcc gtgccggtca
     1561 tgcccgaaag cttgccgtac atgcggaagt agttctggaa ggtgatcgag gccagggtct
     1621 ggttctcgtt ctgaatcctg accccctcct tggcctcgac cgcctggtgc aagccgtcgg
     1681 accagcggcg ccccgacatc aggcggccag tgaactcgtc gacgatgatc acctcaccgt
     1741 tctgcaccac gtactgctga tccctgagga acagattgtg ggcccgcagg gcggcgtaga
     1801 ggtggtggat catcaggatg ttggaggcgt cgtacagact gccgccgtcg gggagcaggc
     1861 cgttggcggc aaggatctcc tcggcgtgct catgcccctc ctcggtcatc aggatctggt
     1921 gacctttttc gtctacccag taatccccgg gaccgttttc ctcggcgcag cgcttgagca
     1981 acggcgccac cttgtccatg cgcacgtaca ggtcagtgtg gtcctccgcc tgtccagaga
     2041 tgatcagcgg cgtgcgggct tcgtcgatca ggatcgagtc gacctcgtcg acgatggcga
     2101 agctgagctt gcgctgcacg cgctcgccgc cggagtagac catgttgtcg cgcaggtaat
     2161 caaagccgaa ctcgttgttg gtcccgtagg tgatgtcggc ggcgtaggcc gcctgcttgt
     2221 cctcgtgcgc catctgcgag aggttcacgc cgaccgtgag gccaagaaag cggtgcaggc
     2281 gtcccatcca ctccgcatcg cggttggcca ggtagtcgtt gaccgtcacc acatgcacgc
     2341 cattgccgga cagcgcattg aggtaggagg ccagcgtcgc gaccagcgtc ttgccttcgc
     2401 cggtgcgcat ttcggcgatc ttcccgtcgt gcagtaccat gccgccgatc aactggacgt
     2461 caaagtgacg catgcccaga gtacgtttgc cggcctcgcg aaccacggcg aaggcttccg
     2521 gcagcagcgc atccagcgtc tcaccattgg ccgcacgggt cttgaattcg gccgtcttgc
     2581 cgcgcaggcc ctcgtcggac agcgcgctga tacctgcttc cagcgcgttg atcttgcgga
     2641 caacctgcga gtactgcttg atcagccgat cattgcggct gccgaaaatc ttcttgagta
     2701 ggccggaaat cattctggga acagtagagg aagttgcgaa gttaatataa ggacccgagc
     2761 caatggcagg gccacttttc ttccgcagtg gggaattgcg gagcggttcg gatggggccg
     2821 ccgaaccggc aggttgacaa gatcagccca atcatgcggc gcgcggcatg gatactacct
     2881 tcaagcgtcg attgacgcaa ttccggcgtc acattctggc ccgtccgggc gtgccgacgg
     2941 cgccgctgcg cctgatcccc gcacgtggac ctgcgctccg agaggggagg actttggacg
     3001 acgagccggt cggtcacgcg gatccgcagt ctgcaccgct cccgactacg aatgccaagg
     3061 ttgacggccc ggcagcgggc ttcctacact gctgaggctg cgttggattt ccagtccgct
     3121 ttcaaacgaa gggagtatgc aggcgatgtc catgccccca gcagtcgagg cctacacggt
     3181 gcaggattac ctgcaatggg aaggcgattg ggaactgatt catggccaac cggtggcaat
     3241 ggtcccgtcg cccagcttcg gtcatcagca ggtcagcgcc gccatctttc ggcaactcga
     3301 cgaatcgctc gacgattgcc cgcattgcca ggcggtgttt gaaatagacg tggaatttgc
     3361 gcaagacacc gtcgtccgcc ccgacgtgct ggtgatctgc tacc
//
LOCUS       DAOSIT010000227         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24331_length_3399_cov_9.495514, whole genome shotgun sequence.
ACCESSION   DAOSIT010000227 DAOSIT010000000
VERSION     DAOSIT010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24331_length_3399_cov_9.495514"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLL14_06495"
     CDS             complement(<1..486)
                     /locus_tag="PLL14_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03610.1"
                     /translation="MKPEQDSYPVFEANQVLTSRHLNDGFNYLDQQERRTRARLIGMG
                     IACGLEVRPGVGGTMIEVGKGCGVTSQGYLIAEPETLNLVTYRAGYRLPDALDDPTFK
                     DAGGQQYPLWELFPAGEPDTVGLGSNAGFLDDKVLLLFVELKQQGLRNCSPNNCDDKG
                     SE"
     gene            complement(510..>3399)
                     /locus_tag="PLL14_06500"
     CDS             complement(510..>3399)
                     /locus_tag="PLL14_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03611.1"
                     /translation="DQSTTTTSLLIRNVALRVFGADAARSATTTQRLKTWLEDRGAGG
                     FIQRYRRKVKAVMAAVDHARAVLNAHRSLDEDFCRVEPIGIEEVAVCADVEVKADADI
                     EAVQARIWFEIERYFNPAIRFHSLQELLDAGKRVEEIFNGPALDNGFITAEDLAAASL
                     KSVLRTSDIVNRLMEIEGVLAVNQLLLTKYDSEGQVIKGAADPAWSAADGKPIFDANK
                     ASAAWLLFVGRQHQPRLYRQVSRFLFYKDGLPFMPRSDEVEDTLSQLRGEAERPKNPF
                     AAADIDIPKGSFRHPEDYQPLQYSFPQTYGIGLDGLPMHASQLRRAQAKQMKAYLMVF
                     EQILGNTLAQLGHTADLFSLDPGIRRTYFVKAFSESLISGFDEITRNLDQAAVEAITE
                     SEAEFLERRNHFLDHLLARFGEQFNDYALLLSKLDGRRLALERLINDKISFLKAYPQI
                     SHDRSRAFNYRFEPCSPANRSGIQSRISLLLGYPDLRFACRVELVAGQYQLDFQLLDR
                     SARIWLAGHQALVAADAAAARELAYRAIIRQLAQPDAYAIAGEGGQFRLQINDQGAAE
                     LGRHPELFASKAQAGALQGELLAWAANERLIVVEHLLLRPKFPGDALFPACAEGACRT
                     CGDEDPYSFRLTFVMPGWTTAYKDNMDLRRFAERTIRQETPAHLVGKICWVGNDGFIE
                     DACAEVVDNLAGLLIAQGMTADGVAPEESDACVGARAIYHVFSSVFRGGYEDKALDHQ
                     HADALRAWLTVEFASAVKASEVGVSMSLGDALWSEIQSLMVEHFHQLALHGWQFERFE
                     EAWLRWLKVNAAFDWPAERLHARVETLLKAHLLAMPADDSSICRGAASILGQYGMSFH
                     SWLDDNLKQGRELAGFSAFIPAVINLPVELSLDASAAAAIGEMLKERYNAYREVSYRL
                     WIVGSLLGKLRNVYPGATLHDCDEGSDFNPVRLDNTALGNYPLRTTLT"
BASE COUNT          657 a         1043 c         1046 g          653 t
ORIGIN      
        1 ctccgacccc ttgtcgtcgc agttgttcgg gctgcaattg cgcaggcctt gctgcttgag
       61 ttccacaaac aacagcagca ccttgtcgtc aaggaaaccg gcgttgctgc ccaggccgac
      121 ggtatccggt tcgccggccg ggaacagttc ccacagcggg tactgctggc cgccggcatc
      181 cttgaaggtc gggtcatcga gcgcgtcggg aaggcggtag ccggcacgat acgtcaccag
      241 gttcagcgtt tcgggttcgg cgatcagata accttgcgaa gtgacgccac agccctttcc
      301 gacctcgatc atcgtaccgc caacgccggg cctgacctcc agaccacagg caatacccat
      361 gccgatcaag cgggcacgcg tccgtcgttc ctgctgatcc agatagttga aaccgtcatt
      421 caaatgcctg ctggtcagca cctggttggc ttcgaaaacc ggataggagt cttgttcggg
      481 tttcatggcg tggtccgtaa tcagaagttt caggtgagag tggtgcgcag cgggtagttg
      541 cccagagcgg tgttgtccag acgcaccgga ttgaagtcgc tgccctcgtc gcaatcatgc
      601 agggttgcac ccgggtagac gttacgcaac ttgccgagca ggctgccaac gatccagagt
      661 cgataggaaa cctcccggta agcgttgtaa cgttccttga gcatctctcc gatggctgcc
      721 gcggcgctgg cgtcgagact cagctcgacg ggcaggttga tcacagcggg aatgaaggca
      781 ctgaacccgg ccaattcgcg tccctgcttc aggttgtcat ccagccagga atgaaatgac
      841 atcccgtact ggcccagaat cgacgccgca ccgcgacaga tgctgctgtc gtcggcgggc
      901 atggcgagca ggtgggcttt gagcagcgtt tccacgcgcg catgcaagcg ctctgcgggc
      961 cagtcaaacg cggcgttgac tttcagccag cgcagccagg cctcttcgaa gcgttcgaac
     1021 tgccagccgt gcagcgccaa ctgatgaaag tgctcgacca tcagcgactg gatctcgctc
     1081 cacagggcat cgccaagcga catcgacaca ccgacttcgg aagccttgac cgcgcttgcg
     1141 aattcgaccg tcagccaggc tctgagggca tcggcatgct gatgatcgag cgccttgtct
     1201 tcatacccgc cgcggaagac gctgctgaag acatggtaga tcgcccgcgc gccgacgcag
     1261 gcgtcgctct cctccggcgc gacgccgtcg gcagtcatgc cttgggcgat caacaagccg
     1321 gccaggttgt cgacgacctc ggcacaggcg tcttcgatga acccgtcgtt gccaacccaa
     1381 cagatcttgc cgaccagatg cgccggtgtc tcctgccgga tggtgcgttc ggcgaagcgg
     1441 cgcaggtcca tattgtcctt gtacgccgtc gtccagcccg gcatgacgaa ggtgagtcgg
     1501 aaggaataag gatcctcgtc gccacaggtc cggcaggcgc cctcggcgca ggcgggaaaa
     1561 agtgcatcgc cgggaaactt cggacgcagc agcaaatgct cgacgacgat caggcgttcg
     1621 ttcgcggccc aggccagcaa ctcgccctgc agcgcacctg cctgggcttt cgaagcaaaa
     1681 agctcggggt ggcggcctag ttcagctgcc ccctggtcgt tgatctgcag acggaactga
     1741 ccaccttccc cggcaatcgc ataagcatcc ggctgcgcca actgccggat gatcgcccgg
     1801 taagcaagtt ccctggccgc agcggcatcg gccgcgacaa gcgcctggtg gcctgccagc
     1861 cagatccttg cgcttcggtc gagcaactgg aaatccagtt ggtattgacc ggcgacgagt
     1921 tcgacccggc aggcaaagcg caggtcggga tagcccagca gcaggctgat gcgtgactgg
     1981 attcccgacc gattggccgg cgaacaaggc tcgaaccggt agttgaaggc tctggaacgg
     2041 tcatggctga tctgtggata ggccttgagg aaggaaatct tgtcgttgat caggcgttcg
     2101 agagccagcc gccggccatc gagcttgctc agcagcagcg catagtcgtt gaactgttcg
     2161 ccgaagcggg cgagcaggtg gtcgagaaag tggttgcgac gctcaagaaa ctccgcttcg
     2221 ctctcggtga tcgcctcgac ggcggcctgg tcgagattcc tggtgatctc gtcaaagccg
     2281 ctgatcaagg actcgctgaa cgctttcacg aagtacgtcc gcctgatccc cggatcgagc
     2341 gagaacaaat cggcggtatg acccagttgt gccagggtgt tgccgaggat ctgctcgaaa
     2401 accatcaggt aggctttcat ctgcttcgct tgcgcccggc gcaactggga agcatgcata
     2461 ggcaggccat cgagaccaat cccgtaggtc tgcgggaagc tgtattgcag tggctggtaa
     2521 tcctccgggt gcctgaagct gcccttggga atgtcgatgt cggcagcggc aaatggattc
     2581 ttcggtcgct cggcttctcc gcgcaactgg ctcaaggtgt cctcgacctc atcactgcgc
     2641 ggcatgaacg gcaggccatc cttgtaaaac aggaaacggg agacctggcg atacagccgt
     2701 ggctggtgct gcctgccgac gaacagcagc cacgccgcgc tggccttgtt ggcatcaaag
     2761 atgggcttgc cgtcggccgc actccaggct ggatcggcgg cgcccttgat cacctggccc
     2821 tctgaatcgt atttggtcaa caacaactga ttgaccgcca gcacgccttc gatctccatc
     2881 aggcgattga cgatgtccga ggtccgcagc accgatttca gcgacgccgc tgccagatct
     2941 tctgccgtga tgaagccatt gtcgagggcg gggccgttga agatttcctc gacgcgcttg
     3001 ccggcatcaa gcaactcctg caaggaatga aagcggatcg ccggattgaa ataccgttcg
     3061 atctcgaacc agatacgcgc ctgcaccgct tcgatgtcgg catcggcctt cacttcgacg
     3121 tcggcgcaaa cggccacttc ctcgatgccg atcggctcga cgcggcagaa gtcttcatcc
     3181 agactccggt gcgcgttgag tactgccctg gcatgatcga cggcggccat caccgctttg
     3241 accttccggc ggtaacgctg gatgaagccg ccggcaccgc ggtcttccag ccaggttttc
     3301 aggcgttggg tcgtggtcgc ggacctggcg gcgtcggcgc cgaacacgcg cagcgcaacg
     3361 ttgcggatca gaaggcttgt cgtggtggtc gactggtcg
//
LOCUS       DAOSIT010000228         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24393_length_3392_cov_6.954450, whole genome shotgun sequence.
ACCESSION   DAOSIT010000228 DAOSIT010000000
VERSION     DAOSIT010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24393_length_3392_cov_6.954450"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..713
                     /locus_tag="PLL14_06505"
     CDS             6..713
                     /locus_tag="PLL14_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOG03612.1"
                     /translation="MIDLYTAATPNGHKISIALEELALPYRVHTLKLSEGEQKQPWFL
                     AINPNGRIPAIVDRAEENFAVFESGAILLYLAEKTGRLMPDDARGRSRAIQWLMFQVG
                     GVGPMMGQANVFFRYFPEKIQPAIDRYQGECRRLFRVLDGHLKEHEYLAGDYSIADIA
                     NWAWVRTHRWSGVDLAELPYLQRWLEQIRRRPAVQQGINTPPSPVDRDADPDQARQFA
                     EEARRMVEMGQSRQSGV"
     gene            780..1403
                     /locus_tag="PLL14_06510"
     CDS             780..1403
                     /locus_tag="PLL14_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HOG03613.1"
                     /translation="MKLYVSPRAPNPRRVLMFIAEKGLTGIEQEIIDIGSGQHLSAAF
                     RARSPLAKLPVLELDDGRSLGETRAICTYLEGLVAEPNLMGNDFEERAFIEMADRRVE
                     LYLFGGIANWIRHTHPGLAPLEQPQFPDFGHSQAEKVREVARWLDRKLASEPYMAGQR
                     FTIADITAFCALEFARGLMKFRPADEGMHRLQAWRDRISQRPSAGVS"
     gene            1543..2091
                     /gene="ppa"
                     /locus_tag="PLL14_06515"
     CDS             1543..2091
                     /gene="ppa"
                     /locus_tag="PLL14_06515"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOG03614.1"
                     /translation="MGLDRVPSGKALPNDFNVIIEIPMHADPVKYEVDKDSGAVFVDR
                     FMSTAMHYPCNYGYIPHTIAGDGDPVDVLVVTQFALHPGVVVRCRPIGMLAMEDEAGQ
                     DAKLLAVPVDSLTSMYRDIESPRDFPQVILDQIAHFFAHYKDLERGKFVKVAGWLGSD
                     EAKKEIMEGVRAYENAKEKPAF"
     gene            2156..>3392
                     /locus_tag="PLL14_06520"
     CDS             2156..>3392
                     /locus_tag="PLL14_06520"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOG03615.1"
                     /translation="MSLKIAIAQINATVGDFAGNRARILGFAERARQQGADLLLTPEL
                     ALCGYPPEDLLLRADFCETCERELDALAKSLQGIAVLVGHPEKRGPHCYNAATLLNDG
                     QRLATYYKQRLPSYEVFDEERYFDSGDGPCVLTLKGIRCGVNICADVWEPGAADLARA
                     AGAAILLVLNASPYHMGKRERRTEVLRQRIATTGLPVVYANLAGGQDELVFDGGSFVL
                     DSRGQLCCQLPQFEEALAVVDFVDGEPQPGAIVAAPCLEAEVYQALVLGVRDYLGKNG
                     FPGAIIGLSGGIDSALTLCIAVDALGADKVRAVMMPSPYTAELSLSESREMVRLLGVR
                     YDEIAIEPAMLTLGSMLEKQFAGLPADTTEENLQARIRGMILMALSNKTGSLVLTTGN
                     KSEMAVGYCTLYGDMAGGFA"
BASE COUNT          663 a         1065 c         1021 g          643 t
ORIGIN      
        1 ccagaatgat cgatctctac accgccgcga cacccaacgg acacaaaatc tcgattgccc
       61 tggaggaact cgctctgccc tacagggtac acacgctgaa gctgtcagag ggtgagcaga
      121 agcaaccctg gtttctggcg atcaacccga acgggcgaat cccggcgatc gtcgatcgcg
      181 ccgaagagaa tttcgcggtc tttgaatcgg gcgcgattct cctgtatctg gctgaaaaaa
      241 ccgggcgcct gatgcccgat gacgccaggg gccgatcccg cgccatccag tggctgatgt
      301 tccaggtcgg tggggtagga ccgatgatgg gccaggccaa tgttttcttc cgttatttcc
      361 ccgaaaagat tcagcccgcg atagaccgct atcagggtga gtgccgacga ctgtttcgcg
      421 ttctcgatgg gcacctcaag gagcatgagt acctggccgg tgactattcg atcgccgaca
      481 tcgcgaactg ggcctgggtt cgcacgcatc gctggtcggg cgtggatctc gccgagctac
      541 cctatctgca acgctggctc gagcagatac gccgtcgccc ggcagtgcaa caaggaatca
      601 acacgccacc ctcgccggtc gatcgggatg ccgaccccga ccaggcacgg cagttcgcag
      661 aggaggcgcg ccggatggtc gagatggggc agtcgcgcca gagcggcgtt tgaggcgcgc
      721 ctgctctcgt cacggcatca tgtccaacca gatagcatat tttgaacagt gagggaacaa
      781 tgaaacttta tgtctcacca agagcaccga acccccgccg agtgttgatg ttcattgccg
      841 agaagggttt gaccggcatc gaacaggaga tcatcgacat cggcagtggc cagcacctga
      901 gtgctgcctt tcgcgccagg agtccgctgg ccaagctgcc ggtgctcgaa ctcgacgacg
      961 ggcgctcgct cggtgagaca agagccatct gtacctacct ggaaggcttg gtcgcagaac
     1021 ccaacctgat gggcaatgat tttgaagagc gcgccttcat cgaaatggct gaccgccggg
     1081 tggaactgta cctcttcgga ggcatcgcca actggattcg ccacactcat cccggcctgg
     1141 cgccactcga acagccgcag ttccccgact tcggccactc gcaagccgag aaggtgcgag
     1201 aagtggcgcg ctggctggat cgcaaactcg ccagcgagcc ctatatggct ggacagcgct
     1261 tcacgatcgc cgacatcacc gctttctgcg ctctcgaatt cgcgcgcgga ctgatgaagt
     1321 tccgtcctgc cgatgagggc atgcaccgtc tgcaggcctg gcgggaccgc atcagccagc
     1381 gccccagcgc cggcgtctca tgacccggca gatgatgcag ctgccgagcc agcgcgcttg
     1441 gcccttgccg tgcagtgaat gaccgaaccg gccgacatga ccggtacaac cctgggcggg
     1501 ggtataattg ctgctcccgc aaccagtaga gagaccacga acatgggcct agatcgcgtg
     1561 ccctccggca aagcacttcc caatgatttc aacgtcatca tcgagattcc gatgcatgcc
     1621 gatccggtga aatacgaagt ggacaaggac agcggcgcgg tcttcgtcga tcgtttcatg
     1681 tccaccgcca tgcactaccc ctgcaactat ggctacattc cacacaccat tgccggtgat
     1741 ggtgacccgg tcgatgtcct ggtggtcact cagttcgccc tgcaccccgg ggtcgtcgta
     1801 cgctgccggc cgatcggcat gctggccatg gaagacgaag caggacagga tgccaagctg
     1861 ctcgcggtcc cggttgatag cctcacgtcg atgtatcgcg acatcgagtc accgcgcgac
     1921 tttccccagg tcatcctcga tcagatcgca cacttctttg cccactacaa ggacctcgaa
     1981 cgtggcaagt tcgtcaaggt cgccggctgg ctgggcagcg acgaagccaa gaaggaaatc
     2041 atggaaggcg tgcgggccta cgagaatgcc aaagaaaagc cggctttctg aaccgctttt
     2101 ttcgccacgc tgatcggggg gcgcacgtgc gccccttttt ctggtacctg tgcttatgtc
     2161 cctgaagatt gcgattgccc agatcaatgc cacggttggc gacttcgccg gcaaccgcgc
     2221 gcggatcctc ggttttgccg agcgtgcccg gcaacagggt gccgacctgt tgctgacacc
     2281 cgaactggcg ctctgcggtt atcctcccga ggatctgctg ctgcgcgccg acttctgcga
     2341 aacctgtgaa cgcgaactcg atgccctggc caaaagcctg caaggcattg ccgtcctcgt
     2401 cggtcacccg gaaaagcgcg gcccgcactg ctacaacgcc gcaacgctcc tcaacgatgg
     2461 acagcggctg gcaacctatt acaagcagcg tttgcccagc tacgaggtct tcgacgaaga
     2521 gcggtatttc gactctggtg acgggccttg tgtcctgacg ctcaaaggca ttcgctgcgg
     2581 cgtcaacatc tgcgccgacg tctgggagcc tggcgccgcc gatctggcgc gcgcggccgg
     2641 agcggcgata ttactggtgc tcaatgcctc cccataccac atgggtaaac gcgagcgacg
     2701 taccgaagtc ctgcgtcaac gtatcgccac taccggcctg ccggtggttt acgcaaacct
     2761 ggcaggcgga caggacgaac tggtcttcga cgggggttcg ttcgtgcttg actcacgcgg
     2821 gcaactgtgt tgtcagttgc cgcagttcga ggaagcgctg gcggtggtcg atttcgtcga
     2881 tggcgagcca cagccgggtg ccatcgttgc cgcaccctgc ctggaagccg aggtctacca
     2941 ggcgctggtg ctcggggtgc gcgactacct tggcaagaac ggttttcccg gcgccatcat
     3001 cggcctttca ggtggcatcg actcggcgct gacgctgtgc atcgcggtcg acgctctcgg
     3061 cgccgacaag gtgcgggcgg tgatgatgcc ttccccgtac accgccgaac tgagcctctc
     3121 ggagtcacgt gagatggtgc gcctgctcgg cgtccgctac gacgaaatcg ccatcgagcc
     3181 ggccatgctg accctcggca gcatgctcga gaagcagttt gcaggcctgc cggccgatac
     3241 caccgaagaa aatctgcagg cccggattcg tggcatgatc ctgatggccc tctcgaacaa
     3301 gaccggttcg ttggtcctga ccaccggcaa caagtccgaa atggcggtcg gttactgcac
     3361 gctctacggc gacatggcgg gcggtttcgc gg
//
LOCUS       DAOSIT010000229         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24433_length_3388_cov_9.697870, whole genome shotgun sequence.
ACCESSION   DAOSIT010000229 DAOSIT010000000
VERSION     DAOSIT010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24433_length_3388_cov_9.697870"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLL14_06525"
     CDS             complement(<1..1316)
                     /locus_tag="PLL14_06525"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG03616.1"
                     /translation="MPTESRKFRRRCANALRFLAIDAVQAANSGHPGMPMGMADLAEV
                     LWRRHLKHNPANPQWVDRDRFVLSNGHGSMLIYALLHLSGYDLPIDELKRFRQLHSRT
                     PGHPELGITPGVETTTGPLGQGLANAVGMALAERLLAETFNRNGHTIVDHYTYAFIGD
                     GCLMEGISHEACSLAGRLSLSKLIVFYDDNDISIDGHVQPWFADDTPKRFEAYGWRVV
                     PHCDGHDVDSIEAAIRIARTQTGRPTLICCKTMIGFGSPNKGGTHDVHGAPLGAGEIV
                     NTRRQLEWPFAPFEIPDDVRAGWDARARGEAAEYAWEESFSAYRAAYPELAAEFARRM
                     RGELPADWDDRVAALLDRVAAQTGDVATRKASQNVIEAIAPALPELLGGSADLTGSNL
                     TNWSGTRPANREAGGNYVNYGVREFGMTAIANGLALHGGFIPYSAT"
     gene            complement(1374..>1844)
                     /locus_tag="PLL14_06530"
     CDS             complement(1374..>1844)
                     /locus_tag="PLL14_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOG03617.1"
                     /translation="AVTTDSVDVGRYVDLCIGVVPIMNLEWIQKLHRDKAQRGYSTEA
                     VMDTILRRMPDYIHYICPQFSRTHVNFQRVPTVDTSNPFIAQDIPSPDESMLVIRFAN
                     PKGIDFPYLLSMLHDSFMSRPNIIVCPGGKMGLAMQIIFTPMIMQLMDKKRRTR"
     assembly_gap    1845..1944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2002..3084)
                     /locus_tag="PLL14_06535"
     CDS             complement(2002..3084)
                     /locus_tag="PLL14_06535"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOG03618.1"
                     /translation="MLCGRTNVNKFLIEEQRRNPALVGDFSMLISDVVRSCKAIAQGV
                     SRGSLAGVLGDEGRDNVQGEAQKKLDVLANQAFIHHCEWGGHLAAMASEEMDDIYPIP
                     ADNPRGKYLLVFDPLDGSSNIDVNLSVGSIFSVLRCPENCSGAAPGTDDFLQPGNRQV
                     AAGYALYGPSTMLVLTVGNGTHGFTLDRDIGEFLLTHPNLLISPDTREFAINASNERF
                     WEPPVQRYVSECLAGKTGIRGKDFNMRWVASMVAEVHRILMRGGVFMYPRDSKDLSKP
                     GRLRLMYEANPMAMIVGQAGGLASTGNERILDVQPTSLHQRVPVILGSRDEVERIERY
                     HREHESGEDQPFRSPLFSTRTLFRVD"
     gene            3201..>3388
                     /locus_tag="PLL14_06540"
     CDS             3201..>3388
                     /locus_tag="PLL14_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG03619.1"
                     /translation="MRNATIRQLQIFSVAASHLSFARAAENLHLTHAAISLQIKQLEE
                     VSGTLLFERIGKRVFLTEA"
BASE COUNT          645 a          960 c         1042 g          641 t
ORIGIN      
        1 aaagtcgccg agtagggaat gaagccaccg tgcagtgcga ggccgttggc gatcgcggtc
       61 atgccgaatt cacggacccc gtaattgacg tagttgccgc cggcttcacg gttggcaggg
      121 cgggtgccgc tccagttggt gaggttcgat ccggtcagat cggccgaacc accgagcaac
      181 tcgggcagag ccggggcgat ggcttcgatc acgttctgcg acgccttgcg cgttgccacg
      241 tcaccggtct gcgctgcgac gcggtcgagc agtgcggcaa cccggtcgtc ccagtcggcg
      301 ggcaactcgc ctcgcatgcg ccgtgcgaac tcggccgcga gttcgggata tgccgcacgg
      361 tacgcggaga agctctcctc ccaggcgtac tcggcggctt ctccgcgcgc gcgcgcgtcc
      421 cagccggcgc gcacatcgtc cgggatttcg aaaggggcaa acggccattc gagttggcga
      481 cgggtattga cgatctcgcc ggcgccgagc ggggcaccgt gcacgtcgtg ggtgccgccc
      541 ttgttcggcg aaccgaagcc gatcatcgtc ttgcagcaga tcagggtcgg ccgtcccgtc
      601 tgcgttctgg cgatcctgat ggccgcttcg atcgagtcca catcgtgacc gtcgcagtgc
      661 ggtaccacac gccagccgta ggcctcgaaa cgcttcggcg tgtcgtcggc aaaccagggc
      721 tggacgtggc cgtcgatcga aatgtcgttg tcgtcataga agacgatcag tttggacagc
      781 gacaggcgcc cggccagcga gcacgcctca tgcgaaatac cttccatcag gcagccatcg
      841 ccgatgaagg cgtaggtgta atggtcgacg atggtgtgcc cgttgcggtt gaaggtttcg
      901 gccaggagac gttcagcgag agccatcccc acggcgttgg cgaggccctg tccgagcggt
      961 ccggtagtcg tttcaacgcc gggggtgata cccagttcag gatgtccggg ggttctggaa
     1021 tgcagttgcc gaaagcgctt gagttcgtcg atgggcaggt cgtagccgct cagatgcagc
     1081 aaggcataga tgagcatcga accgtgtccg ttggacagca cgaaacggtc gcggtcgacc
     1141 cactgcgggt tggccggatt gtgtttcagg tggcgccgcc acaggacctc ggccaggtcg
     1201 gccataccca tcggcatgcc ggggtggccg gaattcgccg cctggacggc atcgatggcg
     1261 agaaagcgca gcgcgttggc gcagcggcgg cggaacttgc gggattcggt gggcacggaa
     1321 acctccttca aacagggcac gggatcaaac agcaagatga tatgcgctca gcatcagcgc
     1381 gtgcggcgct tcttgtccat caactgcatg atcatcggcg tgaagatgat ctgcatggcc
     1441 aggcccatct tgccacccgg acagacgatg atgttcggtc gcgacatgaa cgaatcatgc
     1501 agcatgctca gcaggtaggg gaagtcgatg cccttcgggt tggcgaagcg gatcaccagc
     1561 atgctttcgt ccggagacgg aatgtcctgg gcaatgaagg ggtttgaagt atcgaccgtg
     1621 ggcacgcgct gaaagttgac gtgtgtcctg gagaactgtg gacagatgta atggatgtag
     1681 tcgggcatcc gccgcaggat ggtgtccatc accgcctcgg tcgaataccc ccgctgcgcc
     1741 ttgtcgcgat gcagcttctg gatccactcg agattcatga tcggcacgac gccgatacac
     1801 aggtccacat aacggccgac atcgacgctg tcggtggtta cggcnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnntttttg atcgacatgg gagtgcctcc gtagctttag
     1981 gggaaatcgt cgctgcgcgc ctcaatcgac ccggaacagg gttcgcgttg agaacagggg
     2041 tgatcggaaa ggctgatcct cgccgctttc atgttcgcgg tggtagcgtt cgatgcgctc
     2101 gacctcgtcg cgcgagccga ggattaccgg cacacgctgg tgcaggctgg tcggctgcac
     2161 atcgaggatg cgctcgttgc cggtcgaagc caggccgccg gcctgtccca cgatcatcgc
     2221 catcgggttg gcctcgtaca tcaggcgcag tcttccgggt ttggacaggt ccttgctgtc
     2281 gcggggatac atgaagacgc caccgcgcat caggatgcga tgcacctcag cgaccatcga
     2341 tgccacccag cgcatgttga agtccttgcc gcgaatgccg gtcttgcccg ccaggcattc
     2401 gctcacgtag cgctgcaccg gcggttccca gaagcgctcg ttggaagcgt tgatcgcaaa
     2461 ctcgcgggta tccggcgaga tcagcaggtt cgggtgggtc agcaggaact cgccgatatc
     2521 acggtcgagc gtgaaaccgt gcgtaccgtt gccgacagtc agcacaagca tcgtcgaggg
     2581 tccgtaaagc gcgtaaccgg cggcaacctg acggttgcca ggttgaagaa aatcgtccgt
     2641 gcccggcgcg gcaccactac aattctcggg gcagcgcaga accgagaaga tgctgccgac
     2701 cgacaggttg acgtcgatat tcgacgaacc gtcgaggggg tcgaaaacca gtagatactt
     2761 gccacgagga ttgtcggcgg ggatgggata aatatcatcc atttcttccg aagccatcgc
     2821 cgccaggtga ccaccccact cgcaatggtg aatgaacgcc tgattggcca gcacatcgag
     2881 ttttttctgg gcttcgccct gcacgttgtc gcgcccctca tcaccgagta caccggccag
     2941 cgagccgcgc gaaacgcctt gcgcaatggc tttgcaggaa cgcaccacat cgctgatcag
     3001 catcgaaaaa tcgccgacca acgccggatt ccggcgctgt tcctcgatca ggaatttgtt
     3061 aacgttggta cgtccgcata gcatggcgat actccagaaa atgaatgtct tgtcgttctc
     3121 gggactgtta ggattctagc ggcctgatgc aaaagcgcta gttaaacgta ttgttcattt
     3181 gatttaagaa aagctaatcg atgagaaacg ccaccatccg tcagctccag atcttttcgg
     3241 tagccgcaag ccatctctcg tttgcgcggg cggccgagaa cctgcatctg acgcacgctg
     3301 ccatctcgct gcaaatcaag cagttggaag aggtcagcgg caccttgctg ttcgaacgga
     3361 tcggcaagcg cgtttttctt accgaagc
//
LOCUS       DAOSIT010000230         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24556_length_3376_cov_8.817224, whole genome shotgun sequence.
ACCESSION   DAOSIT010000230 DAOSIT010000000
VERSION     DAOSIT010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24556_length_3376_cov_8.817224"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>1118
                     /locus_tag="PLL14_06545"
     CDS             4..>1118
                     /locus_tag="PLL14_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOG03620.1"
                     /translation="MRRPLNGRRIALVAHPASVSANLEHALDALATLPDLRITAAFGP
                     QHGLRGDKQDNMAESPEFLDPVHGIPVFSLYGLVRRPTPVMMASFDVLLIDLQDLGCR
                     IYTFITTLRYLLEAAAEHGKAVWILDRPNPVGRPVEGLLLRPGWESFVGAGALPMRHG
                     LTMGELACWFISTLKLDVDCTVIKMHGWNPEAAPGYGWPLGERSWINPSPNAPNVSMA
                     RCYAGTVMLEGTTLSEGRGTTRPLELFGAPDIDARHLISGMQALAPQWLDGCRLRECW
                     FEPTFHKHAGHLCHGVQIHVENGCYRHAQFRPWRLQALAFKTLRQMQPDYALWRDFAY
                     EYEFDRLAIDLINGSPLLREWVDDPAATPTDLDALSRP"
     assembly_gap    1119..1218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1219..2527)
                     /gene="glmM"
                     /locus_tag="PLL14_06550"
     CDS             complement(<1219..2527)
                     /gene="glmM"
                     /locus_tag="PLL14_06550"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG03621.1"
                     /translation="MTRKYFGTDGVRGRVGEIPITPDFVMRLGHATGRVLAARDTARV
                     NIKGSERPAVLIGKDTRISGYMMEAALEAGFSAAGVDVCLVGPMPTPAIAYLTRALRL
                     QAGVVISASHNPYYDNGIKFFSSRGTKLPDELESEIEAALDEPLTCVPSAGLGRARRI
                     DDADGRYIEFCKGTFPFEMDLRGLKIVVDCANGAAYHIAPHVFHELGAEVTSIGTEPN
                     GLNINQDVGATAPAALRAAVIAQGADIGIALDGDGDRVMMVDAMGNLYDGDQLLFAVV
                     RSRLRQGPVAGVVGTLMSNLALEHALGELQVPFVRAAVGDRYVMDMLRQKGWLYGGEN
                     SGHILCLDRHTTGDGIVSALQVLSALREEGGDLLQLLGGLKLYPQRLINVALSKGFPW
                     KQNPLIDATQREVESELNGSGRVLLRASGTEPLLRVMVEGQDSL"
     gene            complement(2745..>3376)
                     /gene="folP"
                     /locus_tag="PLL14_06555"
     CDS             complement(2745..>3376)
                     /gene="folP"
                     /locus_tag="PLL14_06555"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOG03622.1"
                     /translation="PVLEGLDGCGVPLSVDTYKPEVMRAALAHGAAMINDIYALRMPG
                     ALATVADSDCALCLMHMRGEPLSMQEHPCYGNVVAEVQAFLAQRVAAAQEAGIASDRL
                     ILDPGFGFGKTLAHNLELLRHLRTFSCAGLPVLAGISRKSMLGAITGRPVKDRLAASI
                     AAALIAAQCGARILRVHDVAPTRDALAIWQAVEFSPGSGDSPASLPRKI"
BASE COUNT          598 a         1030 c         1040 g          608 t
ORIGIN      
        1 gagttgcgga ggcctctgaa tggccgccgc atcgccctgg ttgcccaccc agcatcggtc
       61 agcgccaatc tggagcatgc ccttgatgcg ctcgccacgc tgcccgatct gcggatcacc
      121 gcggcctttg ggccgcaaca tggcctgcgc ggcgacaagc aggacaatat ggccgaatcg
      181 ccggaattcc tcgacccggt gcacggcatt ccggtgttca gcctctacgg tttggtgcga
      241 cgtccgacgc cggtcatgat ggccagcttc gacgtgctgc tgatcgacct gcaggacctc
      301 ggctgccgta tctacacctt cattaccacg ctgcgctatc tgctcgaagc ggcggccgag
      361 cacggcaagg cggtgtggat actcgatcgg cccaatccgg tgggacgtcc ggttgaaggg
      421 ctgctgctgc gtcccggctg ggagagcttc gtcggcgctg gagcgctacc gatgcgccat
      481 ggcctgacga tgggcgaact ggcgtgctgg ttcatcagca cgctcaagct cgacgtcgac
      541 tgcacggtga tcaagatgca cggctggaac ccggaggctg cgcccggcta cggctggcca
      601 ctcggcgagc ggagctggat caaccccagt ccgaatgctc cgaacgtgtc gatggcgcgc
      661 tgctacgccg gcacggtgat gctcgaaggt acgaccctgt cggaggggcg gggtacgacg
      721 aggccgctgg agctttttgg cgcgccggac atcgacgcgc gtcatttgat cagcggcatg
      781 caggcgctgg cccctcaatg gctggacggt tgccgcctgc gcgagtgctg gttcgagcca
      841 acctttcaca agcacgcggg tcacttgtgt catggtgtgc agattcacgt cgagaatggc
      901 tgctaccggc acgcccagtt tcgtccatgg cgcctgcagg ccctggcctt caagacgctg
      961 cgacaaatgc agccggatta cgccttgtgg cgagatttcg cgtacgagta cgaatttgat
     1021 cgcctggcca tcgacctgat caatggctcg ccgctgctgc gcgagtgggt ggacgacccg
     1081 gcggcgacgc cgaccgacct ggacgccttg agcagaccnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnntc agcgagtcct ggccctcgac catgacgcgc agcagcggtt
     1261 ccgtccccga ggcccgcagc agcacccggc cggagccatt gagttcgctc tccacctcac
     1321 gctgggtggc gtcgatcagc gggttctgct tccatgggaa gcctttgctc agcgccacgt
     1381 tgatcagcct ttgcggatag agcttcaggc cgccgagcag ttgcagcaga tcgccgcctt
     1441 cttcacgcag cgccgagagc acctgcaggg cggaaacgat gccgtcgccg gtggtgtgcc
     1501 tgtcgaggca gagaatgtgg ccggagttct cgccaccata cagccaaccc ttctgtcgca
     1561 gcatgtccat gacgtagcgg tcgccgacgg cggcgcggac aaaaggaacc tgcaactcgc
     1621 cgagggcgtg ttccagggcg agattgctca tcaaggtgcc cacaaccccc gcgacagggc
     1681 cctggcgcaa tcggctgcgg acgaccgcga acaacagttg atcgccatcg tagaggttgc
     1741 ccatcgcgtc gaccatcatc acccggtcgc catccccgtc cagcgcgatt ccgatgtcgg
     1801 cgccctgggc gatgaccgcc gcccgcaagg ctgcgggtgc ggtcgcaccg acatcctggt
     1861 tgatgttcag cccattgggt tcggtgccga tactggtgac ttcggctccc aattcatgaa
     1921 agacatgtgg tgcgatgtgg taagccgctc cgttggcaca atccacgacg atcttcagcc
     1981 cacgtagatc catttcgaaa ggaaaggtgc ccttgcagaa ctcgatgtaa cgaccatccg
     2041 catcatcgat tcgccgcgca cgcccaagtc ctgccgatgg aacacaggtc agcggttcgt
     2101 cgagcgcggc ctcgatctcc gattcgagtt catccggcag cttggtgccg cgggaggaga
     2161 agaacttgat tccgttatcg taataggggt tgtgcgaggc cgaaatgacg acgccggcct
     2221 gcaggcgcag ggcgcgcgta aggtaggcga tagccggcgt tggcatcggg ccgaccagac
     2281 agacatccac accggctgca gaaaagccgg cttcgagcgc agcttccatc atgtagccgg
     2341 aaatgcgggt atccttgccg atcaatacgg ccgggcgttc gcttcccttg atattgacgc
     2401 gagccgtgtc gcgggcggcc agaactcttc ctgttgcgtg tcccaggcgc atgacgaaat
     2461 ccggtgtgat cgggatttct ccgacacgcc cacgcacgcc atccgtacca aaatactttc
     2521 ttgtcacgat cttctctccc atcgagcgca gacaaatcga tgtccgtcaa gacgccattt
     2581 tacgcaaacg gcttgggaga cgctgcttac taccacacaa ttcgtcgctt gtgcgctgtg
     2641 ctggcggcga aaaaggcagg aaaaatgcag gttggcgcgc cagtgtctgc gctgtcgcag
     2701 gcaacaacgt caatgccctc tggactgccc gctggcaggg ctggtcagat cttccgcggg
     2761 aggctggcgg gtgagtcgcc gcttcctggg gagaactcaa cggcttgcca gatggccagg
     2821 gcgtcgcgtg ttggcgcgac gtcatgaacg cgcagaatgc gcgctccaca ctgggcagcg
     2881 atcaaggctg ccgcgatgct ggcagccaag cgatccttga ccggacgtcc ggtgatcgcg
     2941 cccagcatcg acttgcgcga aatacccgcc agcacgggca gtccggcaca cgagaaggtc
     3001 cgcagatgac gcagtagttc gagattgtgt gcgagtgtct tgccgaagcc gaaacccgga
     3061 tcgaggatca ggcgatcgct ggcgatgcct gcctcttgcg ctgcggctac ccgctgtgcg
     3121 agaaaggcct ggacttcggc tacgacattg ccgtagcagg ggtgctcctg catgctcaag
     3181 ggttcgccgc gcatgtgcat caggcagagc gcgcaatcgc tgtcagcgac ggtagccagc
     3241 gctcccggca tgcgcagcgc gtagatgtcg ttgatcattg cagcgccatg cgcgagtgcc
     3301 gcgcgcatca cttccggctt gtaggtgtcg acagagaggg gtacgccgca gccgtccagt
     3361 ccttcaagca ctggaa
//
LOCUS       DAOSIT010000231         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24643_length_3368_cov_6.888017, whole genome shotgun sequence.
ACCESSION   DAOSIT010000231 DAOSIT010000000
VERSION     DAOSIT010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24643_length_3368_cov_6.888017"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2565
                     /locus_tag="PLL14_06560"
     CDS             <1..2565
                     /locus_tag="PLL14_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HOG03623.1"
                     /translation="LGLLFCLLICGIGLLVVLYAAWYLPPGDRLGRFYASLLLFMAAM
                     LGVVLSENLLLLLVFWELTSLSSFLLVAYDSDKHASRSGARMALAVTGGGGLALLAGI
                     LLLGHIAGSHELSVILNAGERIRAHALYAPALILVLLGAFTKSAQFPFHFWLPGAMAA
                     PTPVSAYLHSATMVKAGVFLLARLYPALGGSDLWFWLVSATGAITLVYAAAVAFFRHD
                     IKGLLAYSTISQLGLITLLFGLDTPLSVVAGIFHIINHAIFKASLFMAAGIIDHECGT
                     WDMRRVNGMFRYMPVTATLAIIAAASMAGVPLLNGFLSKEMFFAETVAYQPFAGLGWA
                     LPMFATVAGMLAVAYSSRFVHDVFFNGEPVALSRQPHEPPRWMRAPIELLVLLCLLVG
                     MFPGWTVGPLLDAAAAAALQGPLPDFELALWHGFNRPVLMSLLALVGGVCIYALRAPL
                     FAWQAQLPRWHANGSFERGTRRLAVGARSLIALLDNGSLQRHAALLFAFVVLLGGWAF
                     LSGPTSLVRAPVQAADEAAIAALIALLLGALGATLLYRQRLLALILVSVVGLVVALSF
                     VRFSAPDLALTQLAVETGTIILMLLVLYYLPASAPPQSSAPRLTRDLILALLVGGGLG
                     LTTLQMLGAPVDTISEFYLQQSLAGGGGANVVNVILVDFRGFDTLGEITVLALVALAT
                     HTLLDRLVLVAPSRDIDGRLWAREAHPLFLVMLIRPLLPLSLTVSVYIFLRGHHEPGG
                     GFVAGLITSVALTMQYLGGGMAFAQPRMPRHPTSLLALGLALAAGIGVASWAFARPFL
                     TSAHGQLRLPWLGEVELASAMVFDLGVYIVVVTVVLCVLTGLGRLSLRVPSAREVL"
     gene            2565..2891
                     /locus_tag="PLL14_06565"
     CDS             2565..2891
                     /locus_tag="PLL14_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HOG03624.1"
                     /translation="MEALLAIAVGVLSACGVFLILRARTFPVLLGLTLLSYAVNLFLF
                     ASGRLRFDAAPLIRAGASYTDPLPQALVLTAIVIGFGMTAYLAMLALRALSEAGTDHV
                     DAGREP"
     gene            2888..>3368
                     /locus_tag="PLL14_06570"
     CDS             2888..>3368
                     /locus_tag="PLL14_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HOG03625.1"
                     /translation="MSTHAPILPILIAFAAALLQLACDRLSFQRLVGLLSAALLVLVG
                     IWLTWLADDGQMRVYALGDWPAPFGIVLVVDRLAAAMVLLAAVLGFAALLYASAGFDQ
                     RGKHFHPLFQLQLMGLLGAFLTGDLFNLFVFFEVMLLASYTLLAHGGGLARTRAGISY
                     "
BASE COUNT          407 a         1105 c         1093 g          763 t
ORIGIN      
        1 ctcgggctgc tgttttgtct gctgatctgc ggcatcggcc tgctggtggt gctgtatgcc
       61 gcatggtacc tgccgccagg cgaccgactc gggcgcttct atgcgagcct gctgctgttc
      121 atggcggcga tgctcggcgt cgtgctttcc gagaacctgc tgctgttgct ggttttctgg
      181 gaactcacca gcctgtcgtc gttcctgctg gtggcctacg acagcgacaa gcacgcgtcg
      241 cgaagcggcg cccgcatggc cctggcggtt accggcggcg gcggactcgc actgcttgcc
      301 ggcatcctgc tgctcggaca cattgccggc agccatgagc tgtcggtgat cctgaacgcc
      361 ggcgagcgca ttcgtgcgca cgcgctttat gcgccggccc tgattctggt gttgctcggg
      421 gcgttcacca agtcggcgca gtttcccttc cacttctggc tgccaggcgc gatggcggcg
      481 ccgacgccgg tgtcggctta cctgcattcg gcgacgatgg tcaaggccgg cgtcttcctg
      541 ctcgcccgac tctacccggc gttgggcggc agcgacctgt ggttctggct ggtcagcgcg
      601 acgggcgcga tcacgctggt ctacgctgcc gcagtggcgt tttttcgtca tgacatcaag
      661 gggctgctgg cgtattcgac gatcagccag ctgggcctga tcacgctact gttcggtctc
      721 gacacgccgc tctcggtggt ggccggaatc tttcacatca tcaaccacgc gatcttcaag
      781 gcttcgctgt tcatggccgc cggcatcatt gatcacgaat gtggcacgtg ggacatgcgg
      841 cgcgtcaatg gcatgttcag gtacatgccg gtgactgcca cgctggcgat catcgccgcc
      901 gcgtcgatgg ccggtgtgcc cctgctgaac ggctttctga gcaaggagat gttctttgcc
      961 gaaacggtcg cctaccagcc gttcgcaggc ctcggctggg cgctgccgat gtttgcgacc
     1021 gtcgccggca tgcttgccgt cgcctactcg tcacgcttcg ttcatgacgt gttcttcaat
     1081 ggcgaaccgg ttgccctgtc gcgtcagccg cacgagccgc cacgctggat gcgggctccg
     1141 atcgagttgc tggtcctgct ttgtctgctc gtgggcatgt tccccggttg gaccgtcggg
     1201 ccgctgctcg atgccgccgc cgcggccgcc ctgcaggggc cgctaccgga ttttgaactg
     1261 gccttatggc acggtttcaa tcggccggtg ctgatgagct tgctggcgct ggtcggcggc
     1321 gtctgcatct acgccctgcg cgcgccgctg ttcgcctggc aggcacagtt gccgcgctgg
     1381 cacgcgaacg gttctttcga gcgcggcact cgccggttgg ccgttggcgc ccgctcgctc
     1441 atcgccctgc tcgataatgg ctcgctgcag cgccatgcag cgctgctctt tgctttcgtc
     1501 gtgctgctcg gtggctgggc tttcctttcg gggccgacga gcctcgtccg ggcgcctgtc
     1561 caggccgctg acgaggcggc catcgcggcc ttgatcgcgc tcctgctcgg agcgcttgga
     1621 gcaacgctgc tgtaccggca acgcctcctg gcactgatcc tggtcagcgt cgtggggctg
     1681 gtggtggcgc tgagcttcgt gcgcttctcg gcgcctgacc tggcgctgac ccaactcgcc
     1741 gtggaaaccg ggacgatcat cctgatgctg ctggtcctct actatctgcc ggcgagtgct
     1801 ccgccgcaga gcagtgcccc gaggttgacg cgcgacctga tcctggccct gctggtcggc
     1861 ggtggcctcg ggctgaccac cttgcagatg ctcggcgcac cggtcgatac gatctcggaa
     1921 ttctacttgc agcagtcgct tgccggtggc ggtggggcca atgtggtgaa cgtgatcctg
     1981 gtcgatttcc gtggttttga cacccttgga gaaatcaccg ttctggcgct ggtcgccctc
     2041 gccacgcaca ccctgctcga ccgcctggtc ctggttgccc catcgcggga tatcgacggc
     2101 cggctctggg ccagagaagc gcatccgctg tttctggtca tgctgatccg cccattgctg
     2161 ccattgtcac tgacggtatc ggtctatatt ttcctgcgcg ggcaccatga gcctggtggc
     2221 ggcttcgtcg ccggcctgat caccagcgtc gcgctgacca tgcagtacct cgggggcggc
     2281 atggcctttg cgcagccacg catgccgcgc caccccacca gcttgctggc gctgggcctg
     2341 gcgctggcag ccggcatcgg ggtggccagt tgggcatttg ctcggccctt cctgaccagc
     2401 gctcatggcc agctgcggct accctggctc ggtgaggtcg aactggcttc ggcgatggtt
     2461 tttgatctcg gcgtctatat cgtcgtcgtg accgtcgtgt tgtgcgtgct cacgggactc
     2521 ggcaggctct cgctgcgcgt acccagcgcg cgggaggtgc tctgatggaa gcgttgctgg
     2581 cgatcgccgt cggggtgctg agcgcgtgtg gcgtgtttct catcctgcgc gcacgcacct
     2641 tcccggtcct gctcggactg acgctgctgt cctacgctgt caacctgttt ctttttgcca
     2701 gtggtcgcct gcgcttcgac gcggcacccc tgattcgcgc cggcgccagc tataccgacc
     2761 ccttgccgca agcgctggtg ctcacggcga tcgtcatcgg cttcggcatg accgcctatc
     2821 tggcgatgct ggccttgcgc gccctgtccg aagcgggtac cgatcacgtg gacgcaggtc
     2881 gggaaccttg agcacgcacg cgccgatcct gccgatcctg atcgcgtttg ccgccgcgct
     2941 gctgcaattg gcatgcgatc gactgtcgtt ccagcgactc gtcggactcc tttcggcagc
     3001 cctgctggtg ctggtcggca tctggctgac ctggcttgcc gatgacggcc agatgcgggt
     3061 ctatgcgctt ggcgactggc cggcgccgtt tggcatcgtg ctggtcgtcg atcgcctggc
     3121 ggcggcaatg gtcctgctcg ccgcagtgct gggcttcgcg gcgctgctct acgccagtgc
     3181 cggctttgac cagcgcggca aacatttcca tccgctgttc cagttgcaac tgatgggtct
     3241 gctgggggct ttcctgaccg gggacctgtt caatctcttc gtcttcttcg aagtgatgct
     3301 gctggcttcc tataccctgc tggcacacgg cggcggactg gcaagaaccc gcgcgggaat
     3361 cagctacg
//
LOCUS       DAOSIT010000232         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24666_length_3365_cov_7.221752, whole genome shotgun sequence.
ACCESSION   DAOSIT010000232 DAOSIT010000000
VERSION     DAOSIT010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24666_length_3365_cov_7.221752"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..957)
                     /locus_tag="PLL14_06575"
     CDS             complement(58..957)
                     /locus_tag="PLL14_06575"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG03626.1"
                     /translation="MLGFLPAPLTGLIAAMLLVLNSFFWVPILLLLAVFRLMLPFKMA
                     RLRLDPLLVRIAEAWIVCNKGWMALTQKATWDVQGIAGLDPRGWYLVNCNHQTWADIL
                     VLQYLLTHRIPLLKFFLKQQLLWVPVMGLAWWALDFPFMRRHSEEVLKAHPELRGKDQ
                     EATRLACEKFSLIPTSVVNFCEGTRFTPSKHRLQQSPYRHLLRPKAGGIALALNVMGD
                     KFRAILDVTIVYPDGAPDFWQFLSGGMRRVRVRVQTLPVPRHLASGDYANDPAVREAF
                     QQWMQQIWHEKDAQIETLLNTGP"
     assembly_gap    1180..1189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1190..2057)
                     /gene="ylqF"
                     /locus_tag="PLL14_06580"
     CDS             complement(<1190..2057)
                     /gene="ylqF"
                     /locus_tag="PLL14_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOG03627.1"
                     /translation="MAIQWYPGHMTSARKKAAEAMASIDVVIELVDARLPEASTNPLV
                     RELRQQRQRPCLKVLNKADQADPLVTQAWIEYYNRQDGVLAVALSCKKPADVARLPRL
                     CQTLAPHRSDNTKPLRMMIMGIPNVGKSTLMNVLVKRKIAKVGDEPAVTRAQQTSQID
                     VRHTLTDTPGLLWPRIEYPGDGLMLAASNAVGRNAMIEEEVATFLAGLLLMRYQPLLA
                     KRYGFSPDAASVDAVSLIDEIARVRGLRMRGGGADREKAARVLLQDYRDGLLGRISLE
                     TPSTRSVMLAAER"
     gene            2187..3008
                     /locus_tag="PLL14_06585"
     CDS             2187..3008
                     /locus_tag="PLL14_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG03628.1"
                     /translation="MLAFPEIRLGQSIELLKELHILTRDGKLNQDSRRKLKQVYHLCH
                     FIEPLLAELDTPGSHPTLADHGAGKSYLGFILYDLFFKGRGTGQVYGVEARPELVEQA
                     RSLASRLGFARMSFLNLRVEDAIDSPELPARIDMVTALHACDTATDDAIRFALHKQAR
                     FIILVPCCQAEVAAVLRVRKNESFARTPLSELWRHPIHTREFGSQVTNALRCLQLEAR
                     GYQLTVTELVGWEHSLKNELIIARYTGTARSNAAARLEEILHQLNLNELHARFSP"
     gene            3106..>3365
                     /locus_tag="PLL14_06590"
     CDS             3106..>3365
                     /locus_tag="PLL14_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03629.1"
                     /translation="MENWITRGVAAICAGGSTALFWTFGVFLAVPWREGRLLSLSAVE
                     WQVLGISLVAGLVVAWGALHILAIADQARRPWLYQTLRVALLV"
BASE COUNT          643 a         1106 c          992 g          614 t
ORIGIN      
        1 gtgtgcaggc aaccggatct gtctgcggct cggccctgct aggtgagcag ccttcgctca
       61 tggtccggtg ttgagcagtg tctcgatctg cgcatccttt tcgtgccaga tctgttgcat
      121 ccattgctgg aaagcctcac gaaccgccgg gtcgttggca taatcgccac tcgccaggtg
      181 cctgggcacc ggcaacgtct gtacccgcac ccgaacccgc cgcataccgc cgctgaggaa
      241 ctgccagaaa tccggcgccc catcgggata gacgatggtg acatcgagaa tggcgcggaa
      301 cttgtcgccc atcacgttca gtgccagggc aatgcccccg gccttcggcc tcagcagatg
      361 acgatagggc gactgctgca gccggtgctt gctcggggtg aagcgcgtcc cctcgcagaa
      421 gttgaccacg cttgtgggga tcagcgaaaa cttctcacag gccagacgcg tcgcctcctg
      481 atccttgccg cgcagttccg ggtgcgcctt gagaacttct tcactgtgcc ggcgcatgaa
      541 cggaaaatcg agtgcccacc aggccagtcc cattaccggc acccacaaca actgctgttt
      601 gaggaagaac ttgagcaggg gaatgcgatg cgtcaagagg tactggagta ccagaatgtc
      661 ggcccaggtt tggtgattgc agttcaccag ataccagcca cgcgggtcaa gacccgcgat
      721 tccctggaca tcccacgttg ccttttgcgt cagagccatc cagcctttgt tgcagacgat
      781 ccaggcctcg gctatgcgca ccagcagcgg atcgagacgc aggcgtgcca tcttgaacgg
      841 cagcatcaac cggaacaccg ccaacagcaa caggatcggg acccagaaga agctgttgag
      901 caccagcagc atggcagcga tcaggccggt cagcggcgcg ggcagaaaac cgagcatgac
      961 tacacctgca ggcaagaatc gacgacaccg gcttgagtgc cggcgtcgcc ttcttgcgtc
     1021 ggcagcgaca agtgacgcca agagtagggc atgttcggca tgatgcgcta gggccgaacg
     1081 cctgcagacg ccccgcccgc ccgcagttcg tccgcgtccg gcccaaccag ccggtcatcc
     1141 gccggcgcgc ggccctggcg acgatggcgc tgagcctgcn nnnnnnnnnc ccgttcagcg
     1201 gccagcatca cgctgcgcgt cgacggcgtc tccaggctga tgcgcccaag cagaccatcg
     1261 cggtaatcct gcagcagcac cctggccgcc ttctcgcgat cggcaccgcc gccgcgcatg
     1321 cgcagaccgc gcactctggc gatctcgtcg atgaggctca ccgcatcaac gctggcggcg
     1381 tccggcgaga atccgtaacg cttggccaac aggggctgat aacgcatcag cagcagcccg
     1441 gcgaggaagg tcgccacttc ctcctcgatc atcgcattgc ggcccacggc attgctggcg
     1501 gcaagcatca ggccgtcacc ggggtactcg attctgggcc acagcagccc cggcgtatcg
     1561 gtcagggtat gccgaacatc gatctgtgag gtctgctgcg cacgcgtcac cgctggttca
     1621 tcgccgacct tggcaatctt gcgcttgacg aggacattca tcagtgtcga cttgccgacg
     1681 ttgggaatgc ccatgatcat catccgcaat ggcttcgtgt tgtcgctacg atgcggcgcc
     1741 aatgtctgac acaggcgagg cagccgcgcc acgtccgcag gcttcttgca cgacagcgcg
     1801 acggcgagga cgccgtcctg ccggttgtag tattcgatcc acgcctgggt caccagcgga
     1861 tcggcctggt cagccttgtt gagcaccttg agacacggac gttgacgctg ctgccggagt
     1921 tctcgaacca gcggattggt gcttgcttcg ggcaggcgag catcgaccag ttcgatgacc
     1981 acatcgatcg acgccattgc ctcggccgcc ttcttgcgcg ccgaggtcat gtgaccggga
     2041 taccactgga tggccatgaa cagtccaacg aacgggaaac gggccattat cccacctgtt
     2101 cggtaaccct ggcgcgcgca ttgcgttcac aaccgcctgc aaggcgctag aatgcggatc
     2161 gttcccctcc agtgtcagga aaacgcttgc tggctttccc cgaaatcagg cttgggcagt
     2221 cgatcgaact gctcaaggaa ttgcacatcc tgacgcgcga tggcaaactc aatcaggaca
     2281 gtcggcgcaa gctcaagcag gtttaccacc tgtgccactt catcgagcca ctgctcgccg
     2341 aactcgacac gccgggcagc cacccaaccc ttgccgatca tggggcgggc aagtcctacc
     2401 tcgggttcat cctctacgac ctgttcttca agggccgggg gacgggccag gtctacggcg
     2461 ttgaggctcg tccggaactc gtggagcagg cgcgcagtct ggccagccga ctcggttttg
     2521 cgcgcatgtc gtttctgaac ctgcgcgtcg aagacgccat cgattcgccg gagttgcccg
     2581 cccgcatcga catggtcacc gcgctgcacg cctgtgacac ggccaccgac gacgcgattc
     2641 gcttcgcgct gcacaagcag gcacggttca tcattctcgt cccctgctgc caggctgaag
     2701 tggctgccgt gctgcgcgtg agaaaaaacg agtccttcgc cagaacaccc ctctctgaac
     2761 tctggcgaca tccgatccat acccgcgaat tcggcagcca ggtcaccaat gccctgcgct
     2821 gcctgcaact cgaagcgcga ggctatcaac tgacggtgac tgaactcgtc ggctgggaac
     2881 attcgctgaa aaacgagttg atcatcgcca ggtacaccgg cacggcccgc agcaatgccg
     2941 ctgcgcgcct cgaagaaatc ctgcaccaac tcaatctcaa cgaattgcat gcacgtttca
     3001 gcccttgacc gaggcagcga tgcagcattc gccttggtac cgccaacaaa cttgagaccg
     3061 acccacgttg ccaggacgac caacctggca gatcaaggac ctgccatgga aaactggatt
     3121 acgcgcggcg tcgccgcaat ctgcgccggc ggcagtaccg ccctgttctg gaccttcggc
     3181 gtctttctcg cggtgccctg gcgcgaaggg cggctgctgt cgctcagtgc agtcgaatgg
     3241 caggtcctcg gcatctcact ggtcgccgga ctggttgtcg cctggggtgc cctgcacatt
     3301 cttgcgattg cggaccaggc ccgccggccg tggctctacc agacgctgcg cgtggccttg
     3361 ctggt
//
LOCUS       DAOSIT010000233         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24680_length_3363_cov_9.291415, whole genome shotgun sequence.
ACCESSION   DAOSIT010000233 DAOSIT010000000
VERSION     DAOSIT010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24680_length_3363_cov_9.291415"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..579)
                     /gene="orn"
                     /locus_tag="PLL14_06595"
     CDS             complement(34..579)
                     /gene="orn"
                     /locus_tag="PLL14_06595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOG03630.1"
                     /translation="MTQVANHLVWLDMEMTGLEPERDRIIELAMIVTDSQLVTIAESP
                     AWVVRQDEARLDAMDDWNRKTHARSGLIERVRASVLDEAAVERAALSFLQGLVPQAAS
                     PMCGNSIGQDRRFMVKYMPQLEAWFHYRNLDVSTLKELCRRWQPEVAKGFVKKSDHTA
                     LADIRESIAELKYYREHFIRL"
     gene            623..1861
                     /locus_tag="PLL14_06600"
     CDS             623..1861
                     /locus_tag="PLL14_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOG03631.1"
                     /translation="MPNTFSLLFLVALLLMCVLRLWLAQRQISHVRAHREAVPESFSG
                     HIALGAHQKAADYAVARARFGRVGLAIDVAVLLGLTFGGGLQALHDFWSHRLDGLPYG
                     VALVFSVIALSAVVDLPLTLYHQFVIEEKFGFNRMRLTLFLGDLVKQATLGIVIGTPV
                     LLAVLWLMESMGELWWLYVWLFWCGFNLLLLFIYPTWIAPLFNKFAPLEDDALRTRVE
                     ALLARCGFTTSGLFVMDGSKRSGHGNAYFTGFGKTKRIVFFDTLLGRLQAVEVEAVLA
                     HELGHFRHRHIVKRIGMLFVMSLLFLAILGQLISADWFFNGLGLDARNTALGLVLFFL
                     AGPVFAFFLTPLMSLLSRRDEFEADSYAAGHASAGNLVSALVKLYEDNAATLTPDPLH
                     SLFYDSHPPAALRIARLQDA"
     gene            1887..2228
                     /locus_tag="PLL14_06605"
     CDS             1887..2228
                     /locus_tag="PLL14_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOG03632.1"
                     /translation="MTTQQQLALERCRPLRGSEHLLDLAQRSALLTALPGWQLVGETV
                     LQKEFTFASYAATLLFVNMVAGLAERENHHPDLAVAYGRVKVSYSTHDVGGLSRNDFV
                     CAAKIEALTQF"
     gene            2225..3091
                     /gene="rsgA"
                     /locus_tag="PLL14_06610"
     CDS             2225..3091
                     /gene="rsgA"
                     /locus_tag="PLL14_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG03633.1"
                     /translation="MRLAGTVVAAYGRQYRIELADGTTLLCVPRGKKSELVCGDEVSV
                     QRTSPDQGVISSLELRRSLLYRSDHFKQKLIAANVTQVIIVAATEPAFSDELITRCIV
                     AAESQDIKVLIVLNKCDLLEKVAVAKATLRPFQALGYRVLPLCARNNGASLLPYLAGH
                     TSLLIGQSGMGKSTLTNALVPDACAPTREISQALNAGKQTTTYASLYRPGASSALIDS
                     PGLTEFGLRHLSVEEISQAFVELRPLLGQCRFRDCRHDREPDCSILAAVRHGDIDPRR
                     YAAYRSLCAEIG"
     gene            3110..>3363
                     /locus_tag="PLL14_06615"
     CDS             3110..>3363
                     /locus_tag="PLL14_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOG03634.1"
                     /translation="MSDIIIRRQHGKNLAEARASAEHMAAELKEEFDLDYAWNGNVMR
                     FKRPGVAGELSVNDQTVALNIRLGLLLFALKPAIEREIHR"
BASE COUNT          620 a         1091 c          943 g          709 t
ORIGIN      
        1 ctacgcgcaa cgcaagtcgc cgctgtcacc gcctcaaagc ctgatgaagt gctcgcggta
       61 gtatttcaac tcggcaatcg actcgcgaat atccgccagc gccgtgtggt cggacttctt
      121 gacaaagcct tttgccacct cgggttgcca gcggcggcag agttccttca gggtgctgac
      181 gtcgaggttt cgatagtgaa accaggcctc aagctgcggc atgtacttga ccatgaagcg
      241 acgatcctgc ccgatcgaat tgccgcacat tgggctggcc gcctggggca ccaggccctg
      301 caggaaactc agggcggccc tttcgaccgc ggcttcgtcg agcaccgagg cccggacccg
      361 ttcgatcaac ccggagcggg catgcgtctt tcgattccag tcgtccatcg cgtcgaggcg
      421 agcctcatcc tgacgtacca cccaggccgg cgattcggca atggtcacca gctgactgtc
      481 ggtcacaatc atcgcaagtt caatgatgcg gtcgcgttca ggttccagac cggtcatttc
      541 catgtccagc caaacgaggt gattggcaac ctgtgtcata taatccacct gaactgtaaa
      601 agccctattg tgtcacaggc ccatgcccaa tactttctca ctgctgtttc ttgtcgcctt
      661 gctgttgatg tgtgtcttgc gtctatggct cgcgcagcgt cagatttctc atgtacgcgc
      721 gcatcgggag gcggtacccg agagcttctc tggacatatc gcacttggcg cccatcagaa
      781 agccgctgac tatgccgtgg cacgtgcccg tttcggcaga gtcggtctcg ccatcgatgt
      841 cgccgttctt ctcggcctca cattcggcgg cggcctgcag gcactgcatg acttctggtc
      901 gcatcgcctc gatggtctcc cttacggggt cgcactcgta ttcagcgtga tcgcgctttc
      961 tgccgtcgtc gatctgccgc tcacgctcta tcaccagttt gtgatcgaag aaaagttcgg
     1021 tttcaaccgc atgcgcctca cgctcttcct cggcgacctc gtgaaacagg cgaccctggg
     1081 catcgtcatc ggtacaccgg tgctgcttgc cgtcctctgg ctgatggaga gcatgggtga
     1141 gctgtggtgg ctctacgttt ggctgttctg gtgcggcttc aatctactcc tcctgttcat
     1201 ctacccgacc tggatcgcac ccttgttcaa caagttcgcg ccactcgaag acgacgctct
     1261 cagaacgcgc gtcgaagccc tgctcgcacg ctgcggcttc accacttccg gccttttcgt
     1321 gatggatggc tcgaagcgat cgggccacgg caacgcctat ttcaccggtt tcggcaagac
     1381 caagcgcatc gtcttcttcg acaccctgct cggccgcctg caagccgtcg aggtcgaagc
     1441 cgtactcgcg cacgaacttg ggcacttcag gcaccggcac attgtcaagc ggatcggcat
     1501 gctgtttgtc atgtcgctgc tttttctcgc cattctcggt caactgatca gtgccgactg
     1561 gttcttcaat ggcctggggc ttgatgccag gaatacggcg ctgggactgg tgctattttt
     1621 cctcgccgga ccggttttcg ctttcttcct gaccccgcta atgagcctgt tgtcccgccg
     1681 cgacgagttt gaagccgaca gctatgcggc cgggcatgcc tcggcaggca acctggtgag
     1741 cgcactcgtg aaactgtatg aagataatgc tgccaccttg acacccgacc cgctacactc
     1801 gctgttctac gattcacatc ccccggcggc cctgcgcatt gcccgcctgc aggacgccta
     1861 agccccgaac aggaggaatt gccaccatga ccacgcagca acaactcgcc ctagagcggt
     1921 gccgcccgtt gcggggtagc gagcacctgc ttgatctcgc gcagcgttcg gccttgctga
     1981 ctgccctccc gggctggcaa ctggtcggcg agaccgtact gcagaaggag ttcacctttg
     2041 ccagctatgc agcgaccttg ctgttcgtca acatggtggc aggcctggct gagcgcgaga
     2101 accatcaccc cgaccttgca gtcgcttacg gccgcgtcaa ggtaagttac agtacccatg
     2161 atgttggtgg cctgtcccgc aatgacttcg tctgcgcggc caagatcgag gcgcttaccc
     2221 agttttgaga ctcgctggca ccgtcgtcgc cgcctacgga cggcagtacc ggatcgaatt
     2281 ggccgacggc acgacgctgc tctgcgtccc gcgcggcaag aagagcgaac tggtctgcgg
     2341 cgatgaggtc agcgtgcagc gaaccagccc ggaccagggc gtgatcagct cgctggagtt
     2401 gcgcaggagc ctgctctatc gctcggatca cttcaaacag aagttgatcg ccgcgaatgt
     2461 gacacaagtg atcatcgtcg ccgccaccga acccgcgttt tcggatgaat tgatcacccg
     2521 ctgcatcgtc gccgccgaaa gtcaggacat caaggtgctg atcgtgctca acaagtgcga
     2581 cctccttgaa aaggtagcgg tggcaaaggc aacactgagg ccattccagg cactgggtta
     2641 ccgagtgctg ccgctttgcg cgcgcaataa tggcgccagc ctgctgcctt atctcgctgg
     2701 ccataccagc ctgttgatcg gccagtccgg aatgggcaag tcaacgctga ccaatgccct
     2761 cgtacccgac gcctgcgcgc caacccgtga gatctcgcag gccctcaacg ccggcaaaca
     2821 gaccacgacg tatgccagcc tctatcgccc cggcgccagc tcggcgctca tcgactcgcc
     2881 cggcctgacc gaattcggcc tgcggcatct gtcggtggag gaaatctcgc aggccttcgt
     2941 cgaattgcgg ccactgcttg gccagtgccg cttccgcgac tgccgacacg accgcgaacc
     3001 cgactgcagc attcttgccg cagtccggca cggtgacatc gacccgcgcc gctatgcggc
     3061 ctaccgctca ctctgtgccg aaatcggcta ataccaaagg agccttcgca tgagcgacat
     3121 catcatccgc cgccagcacg gcaagaacct tgccgaggcg cgcgcctcgg ccgagcacat
     3181 ggccgcggaa ctgaaggaag aattcgatct cgactacgcc tggaacggca acgtcatgcg
     3241 tttcaagcgg cctggggtcg ccggagaatt gtcggtaaac gatcagacgg tggctctcaa
     3301 catccgtctc ggattgctgc tcttcgccct caagccggct atagagcgcg agatccacag
     3361 gtt
//
LOCUS       DAOSIT010000234         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24917_length_3337_cov_12.137721, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000234 DAOSIT010000000
VERSION     DAOSIT010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24917_length_3337_cov_12.137721"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="PLL14_06620"
     CDS             complement(<1..95)
                     /locus_tag="PLL14_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOG03635.1"
                     /translation="MLKRISVHQLTVGMYLKEFCGSWMEHPFWRSS"
     gene            complement(210..1376)
                     /gene="metK"
                     /locus_tag="PLL14_06625"
     CDS             complement(210..1376)
                     /gene="metK"
                     /locus_tag="PLL14_06625"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG03636.1"
                     /translation="MSKEYLFTSESVSEGHPDKVADQISDAILDAILAEDPPARVACE
                     TLVSTGLVVISGEITTKAHINYREIAQDAVRRIGYDNSDIGFDYKSCAILTAINRQSP
                     DIAQGVNEGQGLDLDQGAGDQGLMFGYACNETASLMPLPIHYAHRIMERQAEVRRDGR
                     LPWLRPDAKSQLTVRYVDGRPQSVDTIVVSTQHDPEVSHAQISEAVIEEVIKPVVPKE
                     LLTGNTRYLVNPTGRFVVGGPHGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDR
                     SAAYAARYVAKNVVAAGLAERCEVQVAYAIGVARPVSLMVNTFGTGKVSDEIIVDLIG
                     KHFDLRPKGIIQSLDLLRPIYRKTAAYGHFGRDEPEFTWEVTDKAAALRSAAGL"
     gene            1607..2476
                     /locus_tag="PLL14_06630"
     CDS             1607..2476
                     /locus_tag="PLL14_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG03637.1"
                     /translation="MLTLSRLLSRLPLSWLHAFGALLGWLTWLLSPTYRRHLRENMQL
                     ALGEAESRQLRAAAIGEAGKGALELTRIWLRPLAETAARVVEVSGWELVEAAARRGRG
                     ILYLTPHLGCFEITAQYLSTHAPITVLYRPPRQAWLQAMIEAGRARAQLHLAAADLSG
                     VRSLLKALKRGEAVGMLPDQAPKTGEGRWLDFFGKPAYTMTLAARLVDSGASVIMVWA
                     ERLPAGRGYHFHLQEPTRPIRGTIEERAQQINHEIEHLIRQCPSQYLWGYNRYKGRRG
                     SEAPPLPNAGGPA"
     gene            2529..>3337
                     /locus_tag="PLL14_06635"
     CDS             2529..>3337
                     /locus_tag="PLL14_06635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG03638.1"
                     /translation="MHIAFCLYKYFPFGGLQRDFLRVALACQARGHAIRVYTLEWRGD
                     VPAGFELVLVPVRALSNCRRYAKFSAWVAAHLHGNPADRLVGFNKMPGLDVYFAADPC
                     YEDKARTLRNPLYRLSGRYRHFAAYERAVFGAESRTEILMISPLQMPLFQKYYGTPDA
                     RLHLLPPGIAADRRAPTNAAAIRAEFRSEFGIQENDLLLLQIGSGFKTKGLDRSLKAL
                     AALPQTLARRCRLIAIGDDDPKLFRAQAQALAVAERVSILRGRSDILRFLLG"
BASE COUNT          565 a         1108 c         1067 g          597 t
ORIGIN      
        1 gacgagcgcc agaacggatg ttccatccac gaaccgcaga actccttgag gtacatgccc
       61 accgtgagct ggtgaacgct gattcgcttg agcatggcaa tatcgcggtt tacggggctg
      121 cgccgtgaag gctgcagtgt aaacttccgc cgcatcagac acaagaaagg ggccgaatgg
      181 ccccttcccc ttgccgtgct gcgacgagcc tagagaccgg ctgccgaacg cagggcagcc
      241 gccttgtcgg tgacttccca ggtgaactcg ggttcgtcac gaccgaagtg cccgtaggcg
      301 gcggtcttgc gataaatcgg gcgcaacaga tcgagcgact ggatgatgcc tttcggacgc
      361 aggtcgaagt gcttgccgat caggtcgacg atgatttcgt cgctgacctt gccggtgccg
      421 aaagtattga ccatcagcga caccggccgg gcaacaccga tggcgtacgc cacctgcact
      481 tcgcagcgtt cggcgagacc ggcggcgacg acgttcttgg ccacgtagcg ggcggcgtag
      541 gcggccgagc gatcgacttt cgacgggtcc ttgcccgaga acgccccgcc accgtgcctt
      601 gcggcgccgc cgtaagtatc gacgatgatc ttgcggccgg tcagcccgca gtcgccgtgc
      661 ggaccgccga cgacgaaacg cccggtcggg ttgaccagat agcgggtgtt gccggtcagc
      721 agttccttcg gaacgaccgg tttgatgacc tcctcgatca ccgcttccga gatctgcgcg
      781 tgcgagactt cgggatcgtg ctgtgtcgaa acgacgatgg tgtccaccga ctgcggccga
      841 ccgtcgacat agcggacggt aagctggctc ttcgcatccg ggcgcagcca cggcaggcgg
      901 ccgtcgcggc gaacctcggc ctgacgctcc atgatgcgat gcgcgtagtg gatcggcagc
      961 ggcatcagcg acgcggtctc gttacacgcg taaccgaaca tcagcccctg gtcgccggcg
     1021 ccctgatcga gatccaggcc ctgcccttca ttgacgcctt gcgcgatatc cggcgactgg
     1081 cggttgattg ctgtcagaat ggcgcagctc ttgtagtcga agccgatatc ggaattgtcg
     1141 tagccgatac ggcggaccgc gtcctgcgcg atttcgcgat aattgatatg cgccttggtg
     1201 gtgatttcgc cggagatgac gaccagaccc gtggacacca gcgtttcgca ggcgacccgc
     1261 gcgggcggat cctcggcgag aatggcgtca aggatggcat cggaaatctg gtcggcgacc
     1321 ttgtccggat gaccttcgga aaccgattcc gaagtgaaga ggtactcttt gctcacgctg
     1381 cgtctgctcc aataaaaaaa ccccgaaacg tcggcgttgc cggctccggg gttcgagcag
     1441 acgacgcttt agcagtattt ggtggccgat ttcacggctc cttccgccct gcaagttgtc
     1501 ctgattaact cggcggatgg ataattatag tttttctcct gccggctggt caaaccttcc
     1561 tgcagtattc gttcgctctt ctgacgttcc ctggaagtcc tgtcgcgtgc tcactctctc
     1621 ccgtcttctc agccgtctgc cgctgtcgtg gctgcatgcc tttggcgccc tgctcggctg
     1681 gctcacctgg ctactgtcgc cgacctaccg ccggcatctg cgcgagaaca tgcagctcgc
     1741 gctgggggaa gccgagagcc ggcagctgcg cgccgcggcc atcggcgaag ccggcaaggg
     1801 cgcgctcgaa ctgacgcgca tctggctgcg gccgcttgcc gaaacggccg cgcgggtcgt
     1861 cgaggtctcg ggctgggaac tggtcgaggc ggcggcgcgg cgcggcaggg ggatcctcta
     1921 cctgacgccg cacctgggtt gtttcgagat caccgcccag tacctgtcta cgcacgcccc
     1981 gatcaccgtc ctctaccggc cgccccggca ggcctggttg caggcgatga tcgaagccgg
     2041 tcgggcgcgc gcgcaactgc atctggcggc cgccgatctg tcgggcgtac gcagcctgct
     2101 caaggcactc aagcgcggcg aggcggtcgg catgctgccc gaccaggcgc cgaagacggg
     2161 cgaaggtcgc tggctcgact tcttcggcaa acccgcctat accatgaccc tcgccgcgcg
     2221 cctggtcgac agcggcgcca gcgtgatcat ggtctgggcg gaacgcctgc ccgcggggcg
     2281 cggctatcat ttccacctgc aggaaccgac ccgaccgatc cgcggcacga tcgaagaacg
     2341 cgcgcaacag atcaaccacg agatcgaaca cctgatccgc cagtgtccca gccagtatct
     2401 gtggggctac aaccgctaca aggggcgccg cggcagcgaa gcgccgccgc tgccgaacgc
     2461 cggcggaccg gcgtgagcag gcacgcggcg cggcgatcgt cggattgcca ccgcgcggaa
     2521 gggcattgat gcacattgcc ttctgcctgt acaaatactt ccccttcggc ggcctgcagc
     2581 gcgattttct gcgcgtcgcg ctcgcctgcc aggcacgcgg ccacgccata cgcgtctaca
     2641 cgctggaatg gcgtggcgat gtccccgccg gtttcgaact ggtgctggtg ccggtacgcg
     2701 cgctgagcaa ctgccgccgt tatgcgaaat tcagcgcctg ggtcgcggcg catctgcacg
     2761 gcaatcccgc cgaccggctg gtcgggttca acaagatgcc gggcctggac gtctattttg
     2821 ccgccgaccc ctgttatgaa gacaaggcac gcaccctgcg caacccgctg taccgcctct
     2881 cggggcgcta ccgacatttc gccgcttacg agcgcgcggt cttcggagcg gagagcagga
     2941 ccgagatcct gatgatctcg ccgctgcaga tgccgctgtt ccagaagtac tacggcacgc
     3001 ccgacgcgcg cctgcacctg ttgccgccgg gaatcgccgc cgaccggcgg gcaccgacca
     3061 acgccgcggc catccgtgcc gagttccgca gcgagttcgg catccaggag aacgacctgc
     3121 tgctgttgca gatcggctcc ggattcaaga ccaaggggct ggaccgcagc ctgaaggccc
     3181 tcgccgccct gccgcagacc ctggccaggc gttgccgact gatcgccatc ggcgacgatg
     3241 atccaaagct tttccgcgcg caggcgcagg cgctggccgt cgccgagcgc gtcagcatcc
     3301 tgcgcgggcg cagcgacatc ctgcgctttc tgctcgg
//
LOCUS       DAOSIT010000235         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_24976_length_3331_cov_7.902320, whole genome shotgun sequence.
ACCESSION   DAOSIT010000235 DAOSIT010000000
VERSION     DAOSIT010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24976_length_3331_cov_7.902320"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PLL14_06640"
     CDS             complement(<1..149)
                     /locus_tag="PLL14_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOG03639.1"
                     /translation="MRDAAINLRALPEQRDLIDHAATLLGKNRSDFMLEAACDKAQSV
                     VLDQV"
     gene            470..1042
                     /locus_tag="PLL14_06645"
     CDS             470..1042
                     /locus_tag="PLL14_06645"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOG03640.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQEVRVFRNDAITVAEIARLKP
                     AWLVISPGPCAPVQAGVSLAAIREFAGKIPLFGVCLGHQAIGEAFGGRVVHARKLMHG
                     KVSPVHHNQHGVFRGLPSPLTCTRYHSLAVERDSLPDCLEITAWTEDGEIMGLRHRTL
                     AVEGVQFHPESILSESGHDLLKNFLEEHAK"
     gene            1039..2064
                     /gene="trpD"
                     /locus_tag="PLL14_06650"
     CDS             1039..2064
                     /gene="trpD"
                     /locus_tag="PLL14_06650"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOG03641.1"
                     /translation="MKPQDALARVIEHREIFHDEMISLMRQIMGGEVTPVMIAAIIVG
                     LRVKKETIGEISAAAQVMRELSTKVLVGDRTRLVDTCGTGGDGAQTFNISTAAMFVAA
                     AAGARVAKHGGRSVSSQSGSADVLEALGVNINLTPAQVGASIDEVGIGFMFAPNHHSA
                     MKHAAPVRRELGVRTLFNILGPLTNPANAANQVLGVFHPDLVGIQVRVLQRLGSSHAL
                     SVHGREGLDEISISGETLVGELKDGRVEEYAIHPEQFNLPVHDPRVLRVANVEESKAM
                     LLGALENTGGAARDIVALNAGASIYVSGLANTLADGVDQAFEAIASGAARARLDDLVA
                     FTQRFSA"
     assembly_gap    2074..2173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2174..2965
                     /gene="trpC"
                     /locus_tag="PLL14_06655"
     CDS             2174..2965
                     /gene="trpC"
                     /locus_tag="PLL14_06655"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOG03642.1"
                     /translation="MSSDILNRILAVKREEVVAAKAHVPLAALRADAERQPAARDFLG
                     AIRDRVAAGRPAVIAEIKKASPSRGVLRADFRPAEIAGSYQHHGAACLSVLTDRQFFQ
                     GAPAYLQAARSACDLPVLRKDFLVDPYQVHEARAMGADAILLIVAALDLPTLRDFESL
                     ADALGMAVLVEVHDRRELDLALQLRTPLIGINNRNLRSFEVSLQTTLELLPALPADRI
                     VVTESGILSGRDVQLLRDNGVNAFLVGEACMRAVDPGMALAELFS"
BASE COUNT          600 a         1057 c          974 g          600 t
ORIGIN      
        1 aaaacctgat cgagcactac cgactgcgct ttgtcgcagg cggcctcgag catgaagtcc
       61 gaccggttct tacccaagag ggtcgcggca tgatcaatca ggtcccgctg ctcggggagt
      121 gcccgcaggt tgatggcggc gtcacgcatg aaatgctcct ttactgcata cataacagat
      181 acacgtaacg tagcaagacg tgtatttgtt gtcaacacac aacttgggcg attgaaccga
      241 atagagagcc tacaccacga tcgcccgcca gtacgccaaa tcagattgtg tgaacgactc
      301 ccaaaagtac cggcgccgca gttgagcggc gctcgcgtgc aggcgataat atgagttctc
      361 cgtgtgcccg acctcaacgc ggcccgcaag cgaacaaaaa caggataaca aaccggacaa
      421 gattcctgat tcttgtccac aacttctata ttcataggct actcggcata tgctgctgat
      481 gatcgataac tacgactcct tcacctacaa tctcgtgcag tacttcggcg agttggggca
      541 ggaggtcagg gtctttcgta acgatgccat caccgtcgcc gagatcgctc gtctcaaacc
      601 ggcctggctg gtgatatccc cgggcccctg cgcaccggtg caggccgggg tttccctggc
      661 ggcaattcgc gagtttgcgg gcaagatccc gctgttcggg gtgtgcctcg gccatcaggc
      721 gatcggcgag gccttcgggg gccgcgtggt gcatgcgcgc aagttgatgc acggcaaggt
      781 atcgccggtc catcataacc agcacggcgt gtttcgtggt ctgccgagcc cgctgacctg
      841 cacgcgttat cattcgctgg ccgtcgaacg ggattccttg ccggactgcc tggagatcac
      901 ggcctggacc gaagacggcg agatcatggg cttgcgccat cgcacgctgg ccgtcgaggg
      961 ggttcagttt caccccgagt cgatcctcag cgagagtggc cacgacctgt tgaagaactt
     1021 ccttgaggag cacgccaaat gaagccccag gacgccctgg cacgggtcat cgaacaccgc
     1081 gagatttttc acgatgaaat gatctccctg atgcgtcaga tcatgggcgg cgaagtgacg
     1141 ccggtcatga tcgccgcgat catcgtcggc ctgcgcgtga agaaggagac gatcggcgaa
     1201 atctcggccg ccgcccaggt catgcgcgag ttgtcgacca aggtgctggt cggcgaccgc
     1261 acccgcctgg tggacacctg tggcacgggt ggtgacggcg cacaaacctt caacatctcg
     1321 accgccgcga tgttcgtcgc cgcggcggct ggcgcgcgcg tcgccaaaca tggcggccgt
     1381 tcggtctctt cgcagtccgg cagcgccgac gtgctcgaag ccctcggcgt gaatatcaac
     1441 ctgacgccgg cgcaggtcgg cgcgagcatc gatgaagtgg gcatcggctt catgtttgcc
     1501 cccaatcatc acagcgccat gaagcacgcc gcgccggtac ggcgcgaact gggtgtgcgc
     1561 accctgttca acatcctcgg gccgctgacc aacccggcca acgcggccaa tcaggtcctc
     1621 ggcgtcttcc accccgatct ggtcggtatc caggtgcgcg tgctgcagcg gctgggcagt
     1681 tcgcacgcgc tctcggtcca cggccgagaa ggtctcgacg aaatctcgat ctccggcgaa
     1741 acgctggtcg gcgaactgaa ggacggccgc gtcgaggaat acgcgatcca ccccgagcaa
     1801 ttcaacctgc cggtacacga cccgcgcgtt ctgcgggtgg ccaacgtcga ggaatcgaaa
     1861 gccatgttgc ttggcgcgct cgaaaacacg ggcggtgccg cacgcgacat cgtcgcgctc
     1921 aatgccggcg ccagcatcta cgtgagcggt ctggccaaca ccctggccga tggcgttgac
     1981 caggcgttcg aagccattgc ctccggcgcg gcacgcgccc ggctcgacga cctggtggcc
     2041 tttacgcagc gcttttcggc ttgatcggcg gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnatgagca gcgacattct caaccgcatt ctcgccgtca agcgtgaaga
     2221 ggtcgttgcc gccaaggccc acgtgccgct tgcggcactg cgcgccgacg cggaacggca
     2281 gcctgcggcc cgcgattttc tcggtgcgat tcgcgatcgc gtcgccgccg ggcggccggc
     2341 cgtgatcgcc gagatcaaga aagccagccc gtcgcgaggg gtcctgcgcg ccgactttcg
     2401 cccggccgaa atcgccggca gttatcagca tcatggcgcg gcgtgcctgt cggtgctgac
     2461 cgatcgtcag tttttccagg gcgcgccggc gtacctgcag gcggcccgca gcgcctgcga
     2521 tctaccggtg ctgcgcaagg acttcctggt ggacccttac caggtccatg aggcccgcgc
     2581 catgggcgcc gacgccatac tgctgatcgt cgcggcactc gatctgccga cgctgcgcga
     2641 cttcgagtcc cttgccgacg cgctcggcat ggcggtactc gtcgaagtcc atgatcgccg
     2701 cgaactcgat ctcgcgctgc aactgcgaac cccactgatc ggcatcaaca accgcaacct
     2761 gcgcagcttc gaggtcagcc tgcagaccac cctcgaactc ctgcctgccc tcccggccga
     2821 tcgcatcgtc gtcaccgaga gcggcattct cagcggccgc gacgtgcagt tgctgcgtga
     2881 caacggcgtc aacgcctttc tcgtcggcga ggcctgcatg cgcgccgttg accccggaat
     2941 ggccctggcc gagttgtttt cctgaccgtc cgcggacgcg ggcaacccgt gtgcgttcgg
     3001 tcgccagcaa cgccccgaca tccaatcgac tcgcttttgc gcgctgtccg ctcgccgagt
     3061 ggcaggcttg ggggacaaat tgccacgcct gttgcaaaaa cccaacagca agtggcatac
     3121 cgtcggcgaa agcgctcagg gagcttgctc gactctccga cgtttgccag aatcgctgca
     3181 acttctcgtt tctttttcga gaggtccagt ttgatgaagc cgcagtctgg cctcatcgat
     3241 ctcaaaccta gaggagattc acaatgcaaa agaaactcat cgcactggca gtcgccagcc
     3301 tggcctcggg cgctgctttt gcccagacca a
//
LOCUS       DAOSIT010000236         3327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25008_length_3327_cov_8.594438, whole genome shotgun sequence.
ACCESSION   DAOSIT010000236 DAOSIT010000000
VERSION     DAOSIT010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25008_length_3327_cov_8.594438"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="purM"
                     /locus_tag="PLL14_06660"
     CDS             <1..813
                     /gene="purM"
                     /locus_tag="PLL14_06660"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG03643.1"
                     /translation="HDTIGIDLVAMSVNDILVQGAEPLFFLDYFACGKLDVDTAAEVV
                     KGIARGCELAGCALIGGETAEMPGMYPVGEYDLAGFAVGVVEKSRIIDGSTIIPGDVV
                     IGLPSSGAHSNGYSLVRNIFARAKPDLTLAFDGEATLADAILAPTRIYVKPLLALMQA
                     LPVKGLAHITGGGLTGNVPRILPPQVKAEIASAAWPRPKLFDWLQREGGVADSEMHRV
                     FNCGIGMVVVVAREDMQQALEILRDTGEPAAVVIGSIKPRDNGEAGTIVVSG"
     gene            901..1785
                     /locus_tag="PLL14_06665"
     CDS             901..1785
                     /locus_tag="PLL14_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG03644.1"
                     /translation="MPQVSANNLQIEYESFGDESAPTILLIMGLGAQLGRWNIELCQA
                     LVVHGYRVIRFDNRDCGLSSKFDTAGVPDIGAALRRGMPLTAAYTLEDMAADCVGLLD
                     ALGIPKAHIVGASMGGAIAQIVAALYPERTLSLTSIMSTSSHPDLPPPTPAAAQALFA
                     PLPATRDQESLVEDAIRRQLAVASPEFPSCPQRLRSLFVEEHERGFHPRGVTRQLAAF
                     LASGDRRTLLATIRVPTLVLHGADDPLIPVACGYDVAAHIPGARMRVIEGMAHDFPLA
                     LTEVFAEAITTVAKAADH"
     gene            1888..>3327
                     /locus_tag="PLL14_06670"
     CDS             1888..>3327
                     /locus_tag="PLL14_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG03645.1"
                     /translation="MNALLAPEPLIFVDLETSGANFANDRIIEIGLIEVDQSGTREWS
                     MLVNPEKPLSPFITGLTGIDDAMLDSAPPFREVAPALLDRLRGRLFIAHNARFDYGFL
                     KSEFRRLGMDFRAPSLCTVKLSRKLFPQHYRHNLDTLVERHGLSVAGDRHRALADARV
                     LWDLWQCWHRLLPVDAIRDAVTTIVGRPHLPAQIDAALLDDLPEAAGAYALYDEHDHL
                     LLVRHSSNLRQQVLAHFTIGNRDTVLFRNTWRIEWRDAAGELGARLRAIELASLASPD
                     GPAHPATRRGQHNRVAATELCSWQLRKLAEGDFRPQLVFSQDIDFATADDLFGLYPNR
                     REAMRSLRTLADAQHLCPRQLGLEDAAQAGACTAYRQKNCRGVCIGKEAVGLHSVRLM
                     TALAKFKIKAWPYKGPVLLIEQDEFGMREDHHLVDRWRLVATLHSEDALQACLAHGPT
                     TRKFDPEIYRTLNKFLQAGKVRVRPLPPFS"
BASE COUNT          589 a         1069 c         1033 g          636 t
ORIGIN      
        1 cacgacacga tcggcatcga cctggtggcg atgagcgtca acgacatcct cgtgcagggc
       61 gccgagccgc tgttctttct cgattatttc gcctgtggca agcttgacgt cgataccgcc
      121 gcggaagtcg tcaagggcat cgccaggggc tgtgaactcg caggctgtgc actgattggc
      181 ggcgagacgg ccgagatgcc tggcatgtat cccgtgggcg aatacgatct ggcgggtttt
      241 gccgtcggtg tcgtcgagaa gtcgaggatc atcgacggct caacgatcat acccggcgat
      301 gtggtgattg gcctgccgtc atcgggggca cattccaacg gctattcgct ggtgcgcaac
      361 atctttgccc gcgccaaacc cgacctgacc cttgccttcg acggagaagc gacgctcgcc
      421 gacgcgatcc tggcgccgac gcggatctac gtcaagccgc tgctggcgct gatgcaggca
      481 ttgccggtca agggcctggc gcacatcaca gggggcggcc tgaccggtaa cgtcccgcgc
      541 attctgccgc cacaggtcaa ggccgagatc gcgagcgccg cgtggccgcg cccgaaactg
      601 ttcgactggc tgcagcgcga gggcggcgtc gccgacagtg aaatgcaccg cgtcttcaac
      661 tgcggcatcg gcatggtcgt agtcgtcgct cgcgaagaca tgcaacaggc tcttgagatc
      721 ctccgcgaca cgggagaacc ggcggcggtg gtcatcggca gcatcaagcc gcgcgacaac
      781 ggagaagcgg ggaccattgt tgtttccggt tagtggcgat tgcaccgttg catagatgaa
      841 tgctcaagtt tacataatat caattatacg aagtcgacta gctgccggac ctttttctcc
      901 atgccacaag tttcggccaa caatcttcaa atcgaatacg aatccttcgg tgatgagtcg
      961 gcgccgacca ttctgctgat catggggctc ggcgcacaac tgggacgctg gaacattgag
     1021 ctctgccagg cgcttgtggt ccacggttat cgagtcattc gttttgacaa ccgggactgt
     1081 ggcctgtcga gcaagttcga tacagccggg gtgcctgata ttggcgctgc actgcgcaga
     1141 ggcatgccgc tgaccgcagc ttacaccctc gaagacatgg cggcagactg tgtcggcctg
     1201 ctggatgcgc tgggaatccc gaaggcccac attgtcggcg cgtcaatggg tggcgccatc
     1261 gcacagatcg tcgccgccct gtatccagag cgcacgctgt cgctgaccag catcatgtcc
     1321 accagcagtc atcccgacct gccgccacca actcccgcgg cggcacaagc gctgtttgca
     1381 ccgctgccgg cgacgcgcga ccaggaatcg cttgtggagg acgctatcag gcgccagctg
     1441 gcggtggcca gccccgagtt tccaagctgt ccgcagcgcc tgcggagcct gttcgtcgag
     1501 gagcacgagc gcggcttcca cccgcgcggt gtcacgcggc aactggcagc ctttctggcc
     1561 agcggtgatc gacgcacgtt gctggcaacg attcgggtac cgacgctggt cctgcatggg
     1621 gccgacgatc cgctgatccc ggtcgcctgt ggttacgatg tcgcggccca catccctggc
     1681 gcccggatgc gggtgatcga agggatggcg catgactttc ccttggccct gaccgaggtt
     1741 tttgccgagg ccatcactac cgtggcgaag gccgcggacc actgagttgc cgcgccgtcc
     1801 tgtcggtccg gcgagctgct tttcgttgat ggtcgggcac gctgccgtgc tgcgacctcc
     1861 tttggcgccg ccgaggcgaa actgtcgatg aacgccctcc tcgccccgga acccctgatt
     1921 tttgtcgacc tggagaccag cggcgccaat ttcgcgaatg accgaatcat tgaaatcggc
     1981 ctgatcgaag tcgatcagag cgggacacgc gaatggagca tgctggtcaa tccggaaaag
     2041 ccgctttctc cgttcatcac cgggctaacc ggcatagatg acgcgatgct cgactccgcc
     2101 cctccttttc gtgaggtggc tcctgcgctg ctcgaccgtc tgcgcggacg cttgttcatc
     2161 gcccataacg cgcgcttcga ctatggtttc ctgaagtccg agttcaggcg gctggggatg
     2221 gatttccggg cgcccagctt atgcaccgtc aaactgtcgc gcaagctgtt tccccagcat
     2281 taccggcaca acctcgatac cctggtcgag cggcatggtc tcagcgtggc cggtgatcgc
     2341 catcgcgcgc tggccgacgc gcgcgtactc tgggacttgt ggcagtgctg gcaccggctg
     2401 ctgccggtcg atgccatccg ggacgcggtg acgacgattg tcggccgccc gcacctgccg
     2461 gcgcagatcg acgccgcgct gctcgacgat ttacccgaag cggcgggcgc ctacgcgctt
     2521 tacgatgagc acgatcacct gctgctggtc cggcacagca gcaatcttcg ccagcaggtg
     2581 ctggcgcact ttacgatcgg caaccgcgac acggtactgt tccgcaacac ctggagaatc
     2641 gagtggcgtg acgccgccgg cgaattgggg gcacgtctgc gcgcgatcga acttgccagc
     2701 ctggcgtcgc ctgacggccc ggcacatccc gccacgcgtc gcggccagca taatcgcgtg
     2761 gcggccaccg agctctgttc gtggcaactc cggaaacttg cggagggtga cttccgaccg
     2821 caactcgttt tttcacagga tatcgatttc gctacggctg acgacctgtt cggcctttac
     2881 ccgaatcgcc gagaggccat gcgcagcctg cgcacactgg ctgatgccca gcatctgtgt
     2941 ccccgacagc tcggtctcga ggacgccgca caggcgggag cctgcaccgc ctatcggcaa
     3001 aagaactgcc ggggcgtgtg cattggcaag gaagccgtcg ggctgcacag cgtccggttg
     3061 atgactgccc tggccaagtt caagatcaag gcctggcctt acaaggggcc ggtgctgctg
     3121 atcgagcagg atgaattcgg catgcgtgaa gaccaccacc tggtcgaccg ctggcgcctc
     3181 gtggccacgc tacacagcga ggatgccttg caggcgtgcc tggcgcatgg acccacgacc
     3241 cgcaaatttg atcccgagat ctaccgcacc ctcaacaaat ttctgcaggc cggcaaggtc
     3301 cgcgtgcgcc ccctgccccc gttctca
//
LOCUS       DAOSIT010000237         3319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25090_length_3319_cov_8.766544, whole genome shotgun sequence.
ACCESSION   DAOSIT010000237 DAOSIT010000000
VERSION     DAOSIT010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25090_length_3319_cov_8.766544"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLL14_06675"
     CDS             <1..1050
                     /locus_tag="PLL14_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG03646.1"
                     /translation="LLLLLGACGKAPLPPEPARPVLTQVVGDNAGDELLGYSGEVRSR
                     YETPLAFRIAGKITARLVDSGAVVNAGQVLARLDPADARLSATAAAAQLELAEADLRR
                     FRNLQAKHFVSQAALDTRETAFKAAQAQADLAHNQASYTVLRAEQAGIVGLVAVEVGQ
                     VVAAGQTVMRLARADTLEVAIAIPESRMAELRALGAATVELWADRQASYAGLLREVSP
                     VADPVTRTYAARVSIRHPDARVLLGMTATVRFLRREGNARLTVPLTAIFQHHDGPALW
                     VVGSDQTVVLRPVSVAAYREDTAVLDDGVQAGERIVVAGVHKLSSGEQVRVIDPAKPL
                     AEPQPQSDQSDAAAR"
     gene            1047..>3319
                     /locus_tag="PLL14_06680"
     CDS             1047..>3319
                     /locus_tag="PLL14_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG03647.1"
                     /translation="MTLPNLSEWALHHRSLVAYLIVVLMLGGVLSYFRLGRAEDPDFT
                     FKAMVVRTLWPGATAHEVELQVSERIEKRLQEVPWVDVLRSQTRSGESLIIVLLRDDT
                     PKKEVAETWYQVRKKIGDMRHTLPQGVQGPFLNDEFGETFITIYALTGDGFDLAALRH
                     AADGIARELRQVPDVKKIELIGVQEERIYIEVSHAKLATLGLNPLAIFEALQKQNAMT
                     PAGFYETPSDRVRLRVSGDFRSVASIREIGIQADGRLFRLGDIATVHRGFAEPPAPRM
                     RVLGQDAIGIAIAMNRGGDVIRLGDRLHAEAGRLQKQLPLGIDLHVVADQPQIVRQSM
                     NLFMSSLAEAVLIVLMVSFLSLGMRTGAVVALSIPLVLAITFLLMAVFGIDLQRISLG
                     ALVIALGLLVDDAIIAVEMMVVKMEQGWDRFRAATFAYTSTAFPMLTGTLISAIGFMP
                     VGLAKSGAGEYTFSIFAVVSIALLVSWLVAVLFTPYLGDLLLDAEKLRRKALQHGDDT
                     YGTPFYRRVRGTIEWSLRHRWTVIAATAIVFVASLLVLTLGVQKQFFPASSRPELLVD
                     LWLPNGASLKATEAVAREVERTLAEPEMSQSIKYFASYLGNSSPRFYLPLDQQLFNDS
                     FAQFVVTTHGIEAREALKRRLEDRFADAAGQWSGLRTRVLRLENGPPVGFPVVFRVSG
                     DNLAELRRVAAEVALVMRANPHLKEINFDWNEMGKAIRIDIDQDRARALGISSQELAT
                     FLDAMLTGVAVTQMREG"
BASE COUNT          556 a         1051 c         1101 g          611 t
ORIGIN      
        1 ctgttgctcc tgcttggcgc ttgcggcaag gcgccgctac cgccagagcc ggcgcggccg
       61 gtactgacgc aagtcgttgg cgataacgcg ggtgacgaat tgctcggtta ctcgggcgag
      121 gtacgctcgc gctacgaaac gccgcttgcc ttccgcatcg ccggcaagat cactgcccgg
      181 ctggttgact cgggtgccgt agtcaacgcg ggtcaggtcc tcgcccggct cgacccggcg
      241 gacgcgaggc tgtcggcgac ggcggcggcg gcacagttgg aacttgccga ggccgacctg
      301 cgccgtttcc ggaacctgca ggcgaagcac ttcgtcagcc aggccgctct cgatacccgg
      361 gagacggctt tcaaggcagc acaggcgcag gccgatctgg cacacaacca ggcttcttat
      421 acggtcctgc gggccgagca ggcgggtatc gtgggactgg tggcggtcga agtcggacag
      481 gtcgtggcgg cggggcagac cgtcatgcgg ttggcgcgcg cggacaccct cgaagtcgcg
      541 atcgccatcc ccgaatcgcg gatggccgag ttacgcgcgc tcggggcggc cacggtcgag
      601 ctatgggccg atcgtcaggc cagctacgcc ggactgctcc gcgaagtgtc gccggttgcc
      661 gacccggtaa cgcgtaccta cgcggcaagg gtgtcgatac gtcaccccga cgccagggtg
      721 ctgctgggga tgacggcgac ggtgcgcttt cttcgccgcg agggcaacgc ccgcctgacg
      781 gttccgctga cggcgatttt tcaacaccac gacggccctg cgctatgggt ggtcggatcc
      841 gatcagacgg tcgtcttgcg accggtttct gtcgccgcat accgggaaga cacggccgtg
      901 ctcgatgacg gtgtacaggc tggcgagaga atcgttgttg ccggggtcca caagctgagc
      961 agcggcgagc aggtgcgcgt catcgacccg gcgaagccgc ttgccgagcc gcagccgcag
     1021 agtgatcagt ccgacgcagc cgcgcgatga ccctcccgaa tctttccgaa tgggcgttgc
     1081 accaccgctc gctggtcgcc tacctgattg tcgtgttgat gctcggtggc gtgctgtcgt
     1141 acttccggct gggtcgcgcc gaggacccgg acttcacctt caaggccatg gtggtgcgta
     1201 cgctatggcc gggtgccacc gcccacgagg tcgaactgca ggtcagcgaa cgcatcgaga
     1261 agcgactgca ggaggtgccc tgggtcgacg tgctgcgttc gcagacacgc tccggtgagt
     1321 cgctgatcat cgttctcctc cgggacgaca cgccgaaaaa agaggttgcg gaaacctggt
     1381 accaggttcg caaaaagatc ggcgacatgc gccacaccct gccgcaaggc gtgcagggtc
     1441 cgtttctcaa cgacgaattc ggcgaaacct tcatcaccat ctacgccctg accggcgacg
     1501 gcttcgacct cgcggcgctg cgtcacgcgg ccgacggcat cgcccgcgag ctgcggcagg
     1561 taccggacgt caagaaaatc gaactgatcg gtgtgcagga ggaaaggatc tacatcgagg
     1621 tatcgcacgc caagctggcg acgctggggc tgaacccgct ggcgatcttc gaagcactac
     1681 aaaagcagaa cgcgatgact ccggccggct tttacgagac gccgtccgac cgcgtgcgcc
     1741 tgcgcgtatc cggggatttc aggtccgtcg ccagcattcg cgaaatcggc atccaggcgg
     1801 acggccggct gttccggctg ggcgacatcg cgaccgtcca ccggggcttt gcggaacccc
     1861 cggcgccgcg catgcgcgtg ctgggtcagg acgcgatcgg cattgccatc gccatgaacc
     1921 ggggcggcga cgtgatccgc ctgggcgacc gcctgcatgc ggaagccgga cgcctgcaga
     1981 agcagttgcc gctgggaatc gatctgcacg tggtcgccga ccaaccgcag atcgtgcgac
     2041 agtcgatgaa cctcttcatg tcctcgctgg ccgaagcggt cctgatcgtc ctgatggtgt
     2101 ccttcctgag cctcggcatg cgcaccggtg ccgtggtggc gctgtcgatt ccgctggtgc
     2161 tggcgatcac ctttctgctg atggccgtgt tcgggatcga tctgcagcgg atttcactcg
     2221 gcgcgctggt gattgcgttg ggcctgctgg tcgacgatgc gatcattgcc gtcgagatga
     2281 tggtcgtgaa gatggaacag gggtgggatc gcttccgggc ggcgaccttc gcctacacct
     2341 cgacggcttt tccgatgttg accggtaccc tgatttcggc catcggcttc atgccggttg
     2401 gtctcgcaaa gtcgggtgct ggcgaataca ctttttcgat attcgcggta gtcagcatcg
     2461 ccctgctggt gtcatggctg gtggccgtgc tgttcacccc ctatctgggc gatctgttgc
     2521 tggatgccga aaagctgcgc aggaaggctc tgcagcacgg cgacgacacc tatggcacgc
     2581 cattctatcg ccgcgtgcgc ggtaccatcg aatggtcgct gcggcatcgc tggacggtga
     2641 tcgcggccac cgccatcgtg ttcgtggcgt cgctgctggt actgaccctg ggcgtgcaga
     2701 agcaattctt tccggcgtcc agccgccccg agttgctggt cgatctgtgg ttgccgaacg
     2761 gcgcgtccct gaaggccacc gaagcggtgg cccgggaagt cgagcgaacg cttgccgagc
     2821 cggaaatgag ccagtcgatc aagtatttcg cgagttacct cggcaacagt agcccacgtt
     2881 tctacctgcc cctggaccag caactgttca acgacagttt tgcgcaattc gtcgtcacga
     2941 cccatggtat cgaggcgcgc gaggcgctca agcgacggct ggaagaccgc ttcgccgatg
     3001 cggcaggcca atggagcggt ctgcgtacgc gtgtgctgcg tctcgagaac ggcccgccgg
     3061 tcggttttcc ggtggtcttc cgcgtctccg gagacaatct cgccgaactg cgccgggttg
     3121 ccgcggaagt cgccctcgtc atgcgcgcca acccgcacct caaggagatc aacttcgact
     3181 ggaacgagat gggcaaggcg atccgcatcg acatcgacca ggaccgcgcc cgcgccctgg
     3241 gcatcagttc acaggaactc gcgaccttcc tcgatgcgat gctcaccggt gtcgccgtga
     3301 cgcagatgcg cgaaggcga
//
LOCUS       DAOSIT010000238         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25160_length_3312_cov_10.820080, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000238 DAOSIT010000000
VERSION     DAOSIT010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25160_length_3312_cov_10.820080"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLL14_06685"
     CDS             <1..390
                     /locus_tag="PLL14_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HOG03648.1"
                     /translation="LACAWLLPVWEAAMEQPGALNDVQRVRLVRTLEDGLAADTSAER
                     GWLARIELAQQRHPRDTGLQYLAGKACMNRQLWGKAQQLLAQAAAGLQDPELRARAWR
                     ALAELAEQRGDADGAAQAWKAAALVLK"
     gene            complement(461..>3312)
                     /locus_tag="PLL14_06690"
     CDS             complement(461..>3312)
                     /locus_tag="PLL14_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG03649.1"
                     /translation="GQKLLDYEIEIEGREQRKGNIYKAVVTRVEPSLEACFVDYGEDR
                     HGFLPFKEISRQYFAQGVSVSQARIQDVIREGDELLVQVEKEERGNKGAALTTFISLA
                     GRYVVLMPNNPRGGGVSRRIEGDDRAELKEAMDQLEYPNGMSIIARTAGIGRSAPELQ
                     WDLNYLLKLWTAIDGAAKGAKGAFLIYQESSLVIRAIRDYFNSDIGDILIDTDDIYEQ
                     AQQFMSHVMPEHAARVKRYRDDAPLFSRFQIEHQIESAYARTVQLPSGGAIVIDHTEA
                     LVSVDVNSARAIKGGDIEETATRTNLEAADEVARQMRLRDLGGLIVIDFIDMEESKNR
                     REVESRLRDALRQDRARVQFGSISKFGLMEMSRQRLKPALSEGSSIPCPRCGGSGHIR
                     DTESSALQILRIIQEESMKDNTASVMCQVPVDVASFLLNEKRPEIAKIELKQRINVLM
                     VPNKTLETPNYRLERLKHDDPRLDNIEASYKMADEVEDPTAVTRRSQEPTNKQTPVIK
                     GVLPDAPAPVAQPRPEAVKAAPAAPAPTAPPAPAPAAKGFMGWIKSLFGAAPQPQPVP
                     AVAPASAAPAARKEEKREGRGGRDSERRDGRRGEGRGDGRRSDERRGGRDGRRDSRSE
                     GRGEPRPETREGEQRPPRGERGERNGARDRREGSSAMAANGVPADAGVAVTLDNGEPR
                     QERAGRGERGEGRRERGERGEGRRERGERPEGRSGGGARAEDGSDRQTAAANADTPDL
                     VAAAALDAGATDGAGQNARPEGEERRERRSRDRYGRDRRERNGERAGDRERAPREEAT
                     EQDSAMDTGEAREPVARSYFDRPTSSATAEASAMPETSANVAPHQAPVAAPAETPRPT
                     PPVPAIAAASVPSMPAVTRFTLPMDELSQIAERSGLQWVNSDADKVAAVQAAIAAEPA
                     PIHVPRERPPLVISDEGPLVLVETRKDLRNLNLPF"
BASE COUNT          427 a         1087 c         1170 g          628 t
ORIGIN      
        1 ttggcctgcg cctggctgct tccggtctgg gaggccgcga tggagcagcc cggcgcgttg
       61 aacgatgtcc aacgggtccg tctggtgcgg acgcttgaag atgggctggc ggctgacacc
      121 agcgccgagc ggggctggct ggcgcgcatc gaactcgcgc agcagcgcca tccccgcgac
      181 acgggcttgc aatacctggc aggcaaggcc tgcatgaatc gccagctgtg gggcaaagct
      241 cagcagttgt tggcccaggc ggcagcaggc ttgcaggacc cggaactgcg agcgcgtgcc
      301 tggcgtgcgc tggctgagct ggccgaacag cgcggcgacg ccgacggagc cgcacaggcg
      361 tggaaggctg cagcgctcgt cctgaaataa aaaagccgga tggctgccca tccggctaga
      421 tgcactttcg tggggcggtc gaatcggccg ccacgccttt ctagaagggc aggttcaggt
      481 tgcgcagatc cttgcgggtt tcaaccagga ccagcggacc ttcgtcggaa atgaccaagg
      541 gcgggcgttc ccgcggcaca tggatcggcg ccggttcggc ggcgattgcc gcctgcacag
      601 cggcaacctt gtccgcatcg gagttcaccc attgcaggcc cgaccgctcg gcgatctggc
      661 tcaactcatc cattggcagg gtgaagcggg tcacggcggg catggacggc acgctggctg
      721 cggcgatggc gggcactgga ggcgtcggcc tgggcgtttc ggcaggcgcc gcaaccggtg
      781 cctggtgcgg cgcgacgttg gcgctggttt ccggcatggc cgatgcctcg gccgtggccg
      841 acgaggtcgg gcggtcgaaa taggagcgcg cgacgggttc gcgtgcctcg ccggtgtcca
      901 tggcggaatc ctgctcggtc gcttcttctc taggggcccg ttcacgatcg ccggctcgct
      961 cgccgttgcg ttcccggcgg tcgcggccgt aacggtcgcg cgagcggcgt tcgcgccgtt
     1021 cctcaccctc ggggcgtgcg ttctggcctg ctccatcggt ggcgccggca tccagagcgg
     1081 ctgcagcgac caggtcgggc gtgtcggcat tcgccgcggc agtctggcgg tcggaaccgt
     1141 cttcggctcg cgcaccgccg ccactgcggc cttcggggcg ttcgccgcgc tcccggcggc
     1201 cttcacctcg ttcgccgcgt tcacgacggc cttcaccgcg ttcaccccgt ccggcgcgtt
     1261 cctgacgggg ttcgccgttg tccagggtca cggccacgcc ggcatcggcg ggtacaccgt
     1321 tggccgccat tgcggacgac ccttcacgac gatcgcgggc accgttgcgc tcgccacgct
     1381 cgccgcgcgg cgggcgctgt tcgccctcgc gggtttccgg gcgcggctcg ccacggcctt
     1441 cgctgcggct gtcgcgccgg ccgtcacggc cgccacggcg ctcgtcactg cgtcggccgt
     1501 cgccacgtcc ctcaccacga cggccgtcac ggcgttcgct gtcgcggccg ccgcgtcctt
     1561 cgcgtttctc ttctttgcgc gcggccggtg cagccgatgc cggcgcaaca gccgggaccg
     1621 gttgcggctg cggcgctgcg ccgaacagac tcttgatcca gcccatgaag cccttggcgg
     1681 cgggtgccgg tgcagggggc gctgtcggcg cgggggccgc cggggctgct ttcacggctt
     1741 ccggacgcgg ctgcgcgacc ggcgcgggcg catccggcag aacacccttg atgaccggcg
     1801 tctgcttgtt ggtgggttcc tgggagcgac gggtcacggc ggtggggtct tcgacctcgt
     1861 cggccatctt gtagctggcc tcgatgttgt ccagccgcgg atcgtcgtgc ttcagtcgct
     1921 cgagacggta gttgggggtt tccagcgtct tgttgggcac catcagcacg ttgatgcgct
     1981 gcttcagctc gatcttggca atttccgggc gcttctcgtt caacaggaac gaggccacgt
     2041 ccaccggcac ctggcacatc accgaggcgg tgttgtcctt catggactct tcctggatga
     2101 tgcgcaggat ctgcagggcc gagctttcgg tgtcgcggat atggcccgag ccgccgcagc
     2161 gcgggcaggg gatggaggag ccttcgctca gggccggctt cagccgctgg cggctcatct
     2221 ccatcaggcc gaacttgctg atggagccga actggacgcg cgcccggtcc tggcgcagcg
     2281 cgtcgcgcag gcggctttcc acctcgcggc ggttcttgga ctcttccatg tcgatgaaat
     2341 cgatgacgat taggccgccg aggtcgcgca ggcgcatctg gcgggccact tcgtcggcgg
     2401 cttccaggtt ggtgcgggtg gcggtttcct cgatgtcgcc gcctttgatg gcgcgggcgg
     2461 agttgacgtc cacggaaacc agcgcttcgg tatggtcgat cacgatggcg cccccggagg
     2521 gcaactgcac cgtgcgggcg taggctgact cgatctggtg ctcgatctgg aagcggctga
     2581 acaggggcgc gtcgtcgcgg tagcgcttga cccgggcggc atgctcgggc atgacgtggc
     2641 tcatgaactg ctgcgcctgc tcgtagatgt cgtcggtgtc gatgaggatg tcgccgatgt
     2701 cgctgttgaa gtagtcgcgg atggcgcgga tcaccaggct cgattcctgg tagatcagga
     2761 aggcgccctt ggcgcccttg gcggcgccgt cgatggccgt ccacagtttg agcaggtagt
     2821 tcaggtccca ctgcagttcc ggcgccgagc ggccgatgcc ggcggtgcgc gcgatgatgg
     2881 acatgccgtt cgggtattcg agctggtcca tggcctcctt gagctcggcc cggtcgtcac
     2941 cctcgatgcg ccggctcaca ccgccgccgc gcgggttgtt cggcatcagc accacatagc
     3001 gtccggccag cgagatgaag gtggtcagcg cggcgccctt gttgccgcgt tcttcctttt
     3061 ccacctgcac cagcagttcg tcaccttcgc ggatcacgtc ctggatgcgg gcctggctca
     3121 ccgagacgcc ctgcgcaaag tactggcggg aaatttcctt gaacggcagg aagccgtggc
     3181 ggtcttcgcc gtagtcgacg aagcaggctt ccaggctggg ttcgacccgg gtcaccaccg
     3241 ccttgtagat gttgcctttg cgctgttcgc gcccctcgat ctcaatttcg tagtcgagca
     3301 gcttctgccc gt
//
LOCUS       DAOSIT010000239         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25161_length_3312_cov_5.936752, whole genome shotgun sequence.
ACCESSION   DAOSIT010000239 DAOSIT010000000
VERSION     DAOSIT010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25161_length_3312_cov_5.936752"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1461
                     /locus_tag="PLL14_06695"
     CDS             253..1461
                     /locus_tag="PLL14_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03650.1"
                     /translation="MALLAMLTLLALAGLFLFVGQLDAMQSRLLRDQKSVAALAEART
                     ALIGDAVSQPSIYDAGYLRLPDLGEKPGPIPAEGEASVNFAGNNKDLSVVGKFPWQTL
                     RTSSLRDAHGECLWYVLSGRFKIFPLTGVLNWDTQGQIDVIDAGGNVTASNLAALLVA
                     PGRALDGQIRDLADEVYSECGGNYDARNYLDAFDGANAIAGEVNYFAGINHRVAPDTG
                     NKHFVLAETNSYNDRFAYVTVDDIFNAVMRRSDFRVAIQSLLDDPDLRKQVETGYPET
                     VAVSSGAGKEKGSDNIDCNLLSSLDNRGFCRNWKEMLWLTALSLPSPITIDGVLTVST
                     CARVLIFAGRKAAGQIRRSAPDKTLKGNYLEGTNLASFDVPTAIASDFSGASMFDYHN
                     SSADLIRCLQ"
     gene            1458..2210
                     /locus_tag="PLL14_06700"
     CDS             1458..2210
                     /locus_tag="PLL14_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG03651.1"
                     /translation="MNRRCCRVWHDQGFTLTELAVVLVIVSLLIGGLLVPLSAQVDIR
                     STNDTRTALAEIREALLGFAVANGRLPCPALPSTASGVAGAGLEVAPPLPGAGCVSLA
                     GVLPWATLAVSENDAWGRRYSYRVTSAFSRFPVGAATAGFTLSSTGDLTIRPAKGAAL
                     IADRVPAVVISHGKNGNGAYLSQGTQLPVGVDADEQENQLTNTAPGVWIDGPVVAEFV
                     KRAAPTATYDDEVVWISPGVLFNRMISAGKLP"
     gene            2333..>3312
                     /locus_tag="PLL14_06705"
     CDS             2333..>3312
                     /locus_tag="PLL14_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG03652.1"
                     /translation="MSADANFIDRLRNAGVYPTDSPELRLQKSLLFFATGLITLASMV
                     WLLIYWQLGPQFSSTLPFVLQLLLVGNLLVYLKTLNFEVFRVVQLSLFLFMPFVAQWS
                     IGSFITASGISLWALLAPIGAILFIGPRESAAWFFAYVFLTTLSGVFDYYLAEPLNLP
                     AYKVPPQTTAFFFALNFAAVSSIVYLLLRYSDTEKHRAQQHLQEAHRLLQIEQERSER
                     LLLNILPGSIAERLKNSTQTIADGFAEVSVMFADIVNFTRVAEGLTPQQVFAMLNKIF
                     SCFDELAERYGLEKIKTIGDAYMVAAGLNAACSHPTESLADMALEMCRLL"
BASE COUNT          580 a          997 c         1043 g          692 t
ORIGIN      
        1 acgcgcgccg ggaatccggc gcaggtgctg atcgaatcgg gcgacgcgcc ggcggccagg
       61 gcgcgtgtcg gcgagacggt caaccgcaat accggcgagt cgcacgatct gctgaatggc
      121 ggcgagatac gcatgcgaac ccggccgcag gcggcgaaat aggcgatgtc tagaccatca
      181 cagaggactt gtgctctgcc ggggttcgcg cgccggcgac ggagcccggt gggcagctgt
      241 cggcagcacg gggtggcgct gctggcgatg cttactctgc tggcactcgc ggggctgttc
      301 ctttttgtcg ggcagctcga tgcgatgcag tcccgactgc tgcgcgatca gaagtcggtg
      361 gcggcgctgg cggaggcccg gaccgcactg atcggcgacg cggtctcgca accgtcaatc
      421 tacgatgccg gatatctgcg cctgccggat ctgggtgaaa agccgggccc aattcctgcc
      481 gaaggggaag cctcggtgaa cttcgcaggc aacaacaagg acctctcggt ggtcgggaaa
      541 ttcccttggc aaacccttcg tacatcgtcg ttgcgtgatg cacatggcga atgcctatgg
      601 tacgttctgt caggccgctt caagatcttc ccgttgacgg gggtactgaa ttgggacacg
      661 cagggtcaga tcgacgtgat tgatgccggt ggcaacgtca cggcgagcaa tcttgccgcc
      721 ctactggtgg ctccggggcg cgcactcgat ggtcagatcc gcgatctagc tgacgaggtc
      781 tacagcgaat gtggtggcaa ctacgacgcc cgcaattacc tcgatgcttt cgatggtgcc
      841 aatgccatcg ccggagaggt caactacttc gcaggcatca accacagagt cgcgccggat
      901 accggcaaca agcacttcgt gctcgccgag accaattcct acaacgaccg ctttgcgtac
      961 gtcacggtcg atgacatctt caacgccgtg atgcggcgca gtgatttcag ggtcgccatt
     1021 cagagcctgc tcgacgaccc cgacctgcga aagcaggtgg agaccggata cccggaaacc
     1081 gtcgccgtgt cgtctggcgc ggggaaggag aagggtagcg acaatatcga ttgcaatctg
     1141 ctcagcagcc tcgacaaccg gggtttttgc cggaactgga aggagatgct ctggttgacc
     1201 gcgttgtcct tgccgtcgcc gatcacgatc gacggcgtat tgaccgtgtc gacgtgtgct
     1261 cgcgtactga tctttgcagg tcggaaggcc gccggtcaga ttcggcgcag tgcacctgac
     1321 aagaccctga aaggcaatta cctggagggc acaaacttgg cttcgtttga tgtgccgacg
     1381 gccatcgcgt ccgatttcag cggtgcctcg atgttcgact atcacaattc ctctgccgac
     1441 ctgattcggt gcttgcagtg aatcggcgct gctgccgggt ttggcatgac cagggattta
     1501 cgctgaccga actggcggtg gtgctggtga ttgtttcgct gctgatcggc gggctgttgg
     1561 tgcccctgtc tgcacaggtc gatattcgca gcacgaatga cacccgaacg gcattggccg
     1621 aaatccgcga ggcgctcctg ggctttgccg tggccaatgg ccggctaccc tgtccggcgc
     1681 tgccgagcac ggcgagtggc gtcgctggcg ccggacttga ggtggcacca ccacttcccg
     1741 gcgctggttg tgtgagtctt gccggagtgc tgccctgggc gacgctggcg gtcagtgaaa
     1801 acgacgcttg ggggcgacgc tattcgtacc gggtgacgag cgctttcagc cgcttcccgg
     1861 taggggccgc gactgccggt ttcacgctgt cgtcaaccgg ggacctgacc attcgtccgg
     1921 cgaaaggcgc cgcactgatt gctgacaggg tgccggcagt ggtgatttcc cacggcaaga
     1981 atggcaatgg cgcctacctc tcgcagggga cgcagttgcc cgtcggtgtg gatgcggatg
     2041 agcaggaaaa tcagctcacc aatactgcgc cgggggtgtg gattgacggt cccgtggtgg
     2101 ccgaatttgt gaaaagagcc gcgccgactg ccacctacga tgacgaagtg gtgtggattt
     2161 cgccgggggt tctcttcaac cgcatgatca gtgccggcaa acttccctag ccgcgcgtcc
     2221 cggctcgttg gcgagtctgt tcggcggcgg gccagtcaac tcgcattgac caatgcggtg
     2281 ctttcgggtt caataggcgt atgctcgcgt tctgccttcc gccagtgaac cgatgagtgc
     2341 cgacgcgaac ttcatcgatc ggctgcgtaa cgccggcgtt tacccgaccg actcaccgga
     2401 actgcgcctg cagaaatccc tgctcttctt cgccaccggt ctgatcacgc tcgcgtcgat
     2461 ggtctggttg ctgatttact ggcagctcgg gccgcagttc tcgtcgaccc tgcccttcgt
     2521 cttgcagttg ctgctcgtcg gcaatctgct ggtgtacctg aagacgctca atttcgaggt
     2581 ctttcgggtc gtccagttgt cgctgttcct gttcatgccc ttcgtcgcgc aatggagcat
     2641 cggcagtttc atcaccgcca gtggcatcag cctgtgggcc ttgctggcgc cgatcggagc
     2701 gatcctgttc atcgggccga gagagtcggc cgcatggttc ttcgcttacg tctttctcac
     2761 cacgctctcc ggagtcttcg actactacct cgccgaaccg ctgaacttgc ccgcgtacaa
     2821 ggtgccaccg cagaccacgg cgttcttctt tgccctcaac tttgccgccg tgtccagcat
     2881 cgtgtacttg ctgctgcgct actcggacac cgaaaagcac cgcgctcagc agcacctgca
     2941 ggaagcacac cgcctgctgc agatcgagca ggagcgctcg gagcgtttgc tgctgaacat
     3001 cctgccgggt tcgattgccg agcgcctgaa gaacagcacc cagacgattg ccgatggttt
     3061 tgccgaagtt tcggtgatgt tcgccgacat cgtgaacttt acccgcgtcg ccgaggggct
     3121 gaccccgcag caggtgttcg ccatgctcaa caagatcttc tcgtgcttcg acgaactggc
     3181 cgagcgctat ggcctggaaa agatcaagac cattggcgat gcctacatgg ttgccgccgg
     3241 tctcaacgcg gcgtgcagtc atccgaccga gtcgctggcc gacatggcgc tggaaatgtg
     3301 tcgcctgctg ca
//
LOCUS       DAOSIT010000240         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25225_length_3306_cov_10.322670, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000240 DAOSIT010000000
VERSION     DAOSIT010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25225_length_3306_cov_10.322670"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="priA"
                     /locus_tag="PLL14_06710"
     CDS             complement(<1..1439)
                     /gene="priA"
                     /locus_tag="PLL14_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG03653.1"
                     /translation="MTVPQIARVALDVPLRRFFDYRVADGENLSMADIGYRVRVPFGL
                     QHRIGILVELPVATDIPIAQLKAIDGILRDVPTLPPDWFRLTGFCASYYHAPLGQVML
                     STLPAGLRALAPRRPRQRRPEHSLAGTQQAPVLTLEQEAALAAIAAGADGFRAYLLHG
                     VTGSGKTEVYLRLIERALAAGRQSLLLVPEINLTPQLEARLAARFPAAGLVSLHSELG
                     EAARSRNWRAALNGEARIVVGTRLAVFTPLPEIGLIVVDEEHDASFKQQDGIRYSARD
                     LAVFRARDSGIPIVLGSATPSLESWANASGSDTSGRYRLLALRGRAVDGARLPLVRCI
                     DTRVDKPRDGLSSTLLLAIEQRLARAEQSLVFLNRRGYAPVLACAACGWSSSCQRCAA
                     HLVLHLADRRLRCHHCGFETRVPKTCPTCGNQDLGAFGRGTQRIEVALAEHFPQARIL
                     RVDRDSASSRKQWEAVVETIQNGAADILVG"
     gene            complement(1691..2851)
                     /gene="hemE"
                     /locus_tag="PLL14_06715"
     CDS             complement(1691..2851)
                     /gene="hemE"
                     /locus_tag="PLL14_06715"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOG03654.1"
                     /translation="MTRPCNDSFLRALLKEPTEYTPVWLMRQAGRYLPEYCETRRRAG
                     SFLQLCKNPAMACEVTLQPLARYDLDAAILFSDILTVPDAMGLGLYFSDGEGPRFERP
                     LREEWAIRDLSAPDPWDHLRYVMDAVSEIRRALNNSVPLIGFSGSPYTLACYMVEGAS
                     STDYRCIKSMLYNRPDLLHRILSVTADAVSAYLNAQIESGAQAVMIFDSWGGSLSAAA
                     YQEFSLPYLRRIIATLIRQQNGERVPSIVFTKGGGLWLESIADIGCDAIGVDWSIDIG
                     EARRHVGHKVALQGNLDPGVLFATPETIAAEAGKVLASYGADGTGHVFNLGHGISQFT
                     PPENVTALVDAVHTRSRLLRSGTDQKSPQALDLFTETMATSGPPSGSSANPG"
     gene            complement(2905..>3306)
                     /locus_tag="PLL14_06720"
     CDS             complement(2905..>3306)
                     /locus_tag="PLL14_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG03655.1"
                     /translation="GIKMIRAIVLYKQVYRELTRAMHPNAVHPVKIGSELLSSNILFA
                     VLAFGFMYMVCIVSMTLALSFSGLEIVTAFTAVVASINNTGPGLGQVGPATNFGSLSD
                     FQTWVCTFAMLLGRLEIFTLLVVLTPAFWRK"
BASE COUNT          647 a         1023 c         1068 g          568 t
ORIGIN      
        1 ccgaccagga tgtcggccgc gccgttctga atcgtctcca ccaccgcctc ccactgcttg
       61 cggctgcttg ccgaatcgcg gtcgacgcgc aggatgcggg cctgcgggaa gtgctcggcg
      121 agagccactt cgatccgctg cgtcccgcgg ccgaaggcgc cgagatcctg gttgccacag
      181 gtcgggcagg ttttcggcac gcgtgtctcg aaaccgcagt ggtggcagcg caggcgacga
      241 tcggcgagat gcagcaccag gtgggcagca cagcgctggc aggatgaact ccagccgcac
      301 gcagcgcagg ccagcaccgg tgcgtagccg cgccgattga gaaagaccag gctctgctcg
      361 gcacgcgcca gacgctgttc gatggccagc aacaaggtac tggagaggcc gtcccggggt
      421 ttgtcgacgc gcgtgtcgat gcagcgtacg agaggcagac gcgctccgtc cacggcccgc
      481 ccgcgcaagg ccagcagccg gtagcgcccg ctcgtgtcgc tgccactggc gttggcccag
      541 ctctccagtg atggcgtcgc cgaacccaga acaatcggaa tgccgctgtc gcgagcgcgg
      601 aagacggcaa gatcgcgcgc cgaatagcga atcccgtcct gttgcttgaa agaggcgtca
      661 tgctcctcgt cgaccacgat caagccgatc tcgggcagtg gagtgaatac cgcgagccgc
      721 gtgccgacga cgatcctcgc ttcgccgttc agtgcggccc gccagttgcg gctgcgggcg
      781 gcctcgccca gttcactgtg cagactgacc aagccggcgg cgggaaagcg ggcggcaaga
      841 cgcgcttcga gttgtggcgt gaggttgatt tcaggcacca gcaacagcga ctgccgtccg
      901 gcggccagag cgcgctcgat cagccgcaga tagacctcgg tcttgccgct gccagtcaca
      961 ccgtgcagca gataagcgcg gaaaccgtcc gcgcctgcgg caatcgcggc cagcgcagcc
     1021 tcctgttcga gggtcaggac aggggcctgt tgtgtgccgg caagggagtg ttctggccgg
     1081 cgctgccggg gcctccgggg cgccagcgcg cgcaggccgg ccggcagggt cgacaacatc
     1141 acctggccga gtggcgcgtg gtagtagctc gcgcaaaacc cggtcagacg gaaccagtcg
     1201 ggagggaggg tcggcacatc gcgcaggatg ccatcgatcg ctttgagctg ggcgatcgga
     1261 atatctgtcg ccaccggcaa ctcgacgagg attccgatcc tgtgttgcag accaaacggc
     1321 acccgtacac gatagccgat gtccgccatc gacaaattct cgccatcggc gacgcgatag
     1381 tcaaaaaagc ggcgcagcgg cacgtcgagt gcgacgcggg caatttgcgg cacggtcatg
     1441 gggttggcta tgaggttttg cgcggcggga attcgctgtc gacttagcgc gggaagctcg
     1501 cgccagttct ctcaaatgcg ggtaaggtgt tcagttttag acgctttccg tccgatacac
     1561 agatgctgtg gataactctg tggagcaaac cctttgcata ggccgcaaag gccctccctg
     1621 caagggtttc agagtcattg ccggaaaatt aggcaattta aacctcctta caaatcagcc
     1681 agttgccaat ctacccggga tttgcagaag acccgctcgg cggaccggaa gtcgccatgg
     1741 tttctgtgaa taagtcaagc gcctgggggg atttctggtc ggtaccactc cgcaggaggc
     1801 ggctacgggt gtgcaccgca tcgaccagcg cggtcacgtt ttccggcggc gtaaactggg
     1861 agatgccatg acccaggttg aagacatggc cggtgccgtc ggcaccatag ctggccagca
     1921 cttttccggc ctccgcagcg atcgtctccg gcgtggcaaa cagcactccc ggatcgaggt
     1981 tcccctgcaa tgccaccttg tgaccgacat gccgacgtgc ttcgccgatg tcgatggacc
     2041 agtcgacacc gatggcatcg cagccgatat cggctatcga ttccagccat agtccgccgc
     2101 ccttggtaaa cacgatcgat ggcacgcgtt cgccgttctg ctggcgaatg agggtggcga
     2161 tgatgcgccg caggtagggc agcgagaact cctggtacgc cgccgccgaa agcgaccctc
     2221 cccaggaatc gaagatcatg accgcctgcg ctcccgactc gatctgggca ttgaggtagg
     2281 cgctcacggc gtcggcggtc accgacagaa ttcgatgcag caggtcgggg cggttgtaga
     2341 gcatgctctt gatacaccgg tagtcggtac tcgaagcgcc ttcgaccatg tagcaggcca
     2401 gggtgtaagg actgccggaa aaaccgatca agggcaccga gttgttgagt gcgcgcctga
     2461 tttcgctcac tgcgtccatc acgtaacgca ggtggtccca cgggtccggc gcgctgaggt
     2521 cgcggattgc ccactcctcg cgtaacgggc gctcgaagcg gggtccttcg ccgtcactga
     2581 agtacagccc gagccccatg gcatcgggca cggtcaggat atcggagaag aggatcgccg
     2641 cgtccagatc atagcgcgcg aggggctgca gcgtcacctc gcaagccatc gccgggtttt
     2701 tgcacaactg caggaaactg ccggcacgcc tgcgcgtttc acaatactcg ggcaaatagc
     2761 gacccgcctg gcgcatcagc cagaccggcg tatattcggt gggttccttg agcagggcgc
     2821 gcagaaagct gtcattgcag ggtcgggtca cgagaggctc caccggcgag gggaatgggc
     2881 agcgtcgatt atcgcctttc cccgctactt gcgccagaaa gccggcgtca agaccaccag
     2941 cagggtaaag atctccaggc gacccaggag catggcgaaa gtacataccc aggtctggaa
     3001 atcgctcagg ctcccgaaat tggtcgccgg cccgacctgc ccgaggcctg gtccggtgtt
     3061 gttgatgctg gcgaccaccg ccgtgaaggc ggtgacgatt tccaggccgg aaaacgacag
     3121 cgccagggtc atcgacacga tgcacaccat gtacatgaaa ccgaaagcca gcacggcgaa
     3181 gagaatattc gacgaaagaa gttcgctccc gatctttacc ggatggaccg cgttcgggtg
     3241 catcgcgcgc gtcaactccc ggtacacctg cttgtagagc acgatcgcgc ggatcatctt
     3301 gatgcc
//
LOCUS       DAOSIT010000241         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25310_length_3296_cov_7.154582, whole genome shotgun sequence.
ACCESSION   DAOSIT010000241 DAOSIT010000000
VERSION     DAOSIT010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25310_length_3296_cov_7.154582"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="PLL14_06725"
     CDS             complement(<1..154)
                     /locus_tag="PLL14_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG03656.1"
                     /translation="MRLTTKGRFAVTAMIDLALRCSEGPVSLAGISERQKISLSYLEQ
                     LFGKLRR"
     gene            complement(215..967)
                     /gene="cysE"
                     /locus_tag="PLL14_06730"
     CDS             complement(215..967)
                     /gene="cysE"
                     /locus_tag="PLL14_06730"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOG03657.1"
                     /translation="MFSHLREDIQSVFDRDPAARTFWEVLTCYPGVHAMVTHRLAHWL
                     WVHRLRWLGRLVSHLGRFFTGIEIHPGASIGRRLFIDHGMGVVIGETAVIGDDVTLYH
                     GVTLGGTSWNKGRRHPTLANGVVVGAGAKVLGPITIGQSAKVGSNAVVVKDVPARATA
                     VGNPARIIDSAQAQKREEMAGQLGFSAYALAGDQDDPLAKAIHGLLDHAVEVDRRFVL
                     LLSKLEEAGVHVEGPLVTTDQFDPQYLSKIVD"
     gene            complement(1029..>1517)
                     /locus_tag="PLL14_06735"
     CDS             complement(1029..>1517)
                     /locus_tag="PLL14_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOG03658.1"
                     /translation="ALQARQAAADVLAKASTGEVALLFGNETAGLSNAEVQRCRCAIF
                     IPANPEYTSLNLASAVQLLAYELRLAAFDSQPPVVTRAVPFASPAASHQDIERFHAHL
                     ERVMVSSGFLDPQQPKRLLPKLRRLFARAELERDEINILRGLLDAVEKQLAGAAASDS
                     AP"
     assembly_gap    1518..1617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1715..2524
                     /locus_tag="PLL14_06740"
     CDS             1715..2524
                     /locus_tag="PLL14_06740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOG03659.1"
                     /translation="MHPALNIIIKAARRASQIINRASLDLEHLQITTKRQSDFVTEVD
                     RAAEAAIIDVLREAYPEYGILAEESGQTAGSAGGSEYQWIIDPLDGTTNFIHGLPQYA
                     VSIALAHRGQVTQAVVYDTVRNEMFTASKGGGAFLNERRIRVSKCLRLEEALIGTGFP
                     YRIYDHIDAYLAIFKELARKSAGIRRPGAAALDLAYVACGRFDGFWEFGLSPWDIAGG
                     ALLISEAGGLISDLAGNETYLQSGNLVAGNPKVFAQLLQVIDGHRTPGLMA"
     gene            complement(2808..>3296)
                     /locus_tag="PLL14_06745"
     CDS             complement(2808..>3296)
                     /locus_tag="PLL14_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014292986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HOG03660.1"
                     /translation="LSHQAVPGLESTPLCLHAAYGIREILTAVNWLTAERRTPFRAGV
                     LALHEHKIELLFVTLDKRVGYHQRIAYHDFAISTERFHWQSQNSAAPDTPSGRRYLDS
                     SSNGWSFQLFVRCARGNPYRACGPVTLEHAEGARPMTIYWKLSVPLPLRLFQEFSILR
                     GE"
BASE COUNT          654 a         1031 c          950 g          561 t
ORIGIN      
        1 agcggcgcag cttgccgaac agttgttcga gatacgaaag cgagattttc tgccgctcgc
       61 tgatcccggc cagagacacc ggcccctcgc tgcagcgcag tgccaggtcg atcatcgcgg
      121 ttactgcaaa acgtcctttg gtcgtcagtc tcatggttcc tccatgtatc ataatagttg
      181 attggatcgg tatactatag cgacgcagcg tagcttagtc aactatttta ctcaagtatt
      241 gcgggtcgaa ctggtcggta gtgaccagcg gcccctcgac atgcacgccg gcttcctcaa
      301 gcttgctgag cagcagcaca aagcgccgat cgacctcgac cgcatggtcg agcaagccat
      361 gaatcgcctt ggccaagggg tcatcctgat cgcccgccag cgcgtacgcc gaaaacccca
      421 gttgcccggc catctcctcg cgcttctgcg cctgcgcact gtcgatgatg cgcgccggat
      481 tgccgaccgc cgttgcccgc gccggcacgt ccttgacgac cacggcattg gacccgacct
      541 tggccgactg cccgatggtg atcggtccca gtaccttggc gccggcgccg acgaccaccc
      601 cgttggccag cgtcgggtgg cgccggccct tgttccagga agtgccaccg agggtaacgc
      661 cgtgatagag cgtgacgtcg tctccgatca ccgctgtttc accgatcacc acacccatgc
      721 cgtgatcgat gaacaggcgg cgtccgatgc ttgctccggg atggatctcg atgccggtaa
      781 agaagcgccc gaggtgcgaa accagacgcc ccagccaccg caggcgatgc acccacaacc
      841 agtgcgcaag acggtgcgtg accatcgcat gcacgcccgg gtagcaggtg agaacctccc
      901 agaacgtacg ggcagcgggg tcacggtcaa aaaccgattg aatgtcttca cgcagatggc
      961 tgaacatgga acgagagtcc tggaacggtc acccctcaat gccggggcgc tggtacaata
     1021 ccatactatt acggtgcact atccgacgca gcggcaccgg caagctgctt ctcgaccgcg
     1081 tcgagcagtc cgcgcaggat gttgatctcg tcacgttcga gctcggcacg cgcgaaaaga
     1141 cgacgcagct tgggcaacag ccgtttgggc tgctgcggat cgagaaagcc gctgctcacc
     1201 atcacccgct ccagatgcgc atggaagcgt tcgatgtcct ggtggcttgc cgccggcgag
     1261 gcaaagggta cggcccgggt caccaccggc ggctggctgt cgaaggcagc cagccggagt
     1321 tcgtaggcca gcagttgcac cgccgaggcg aggttgagcg aggtgtactc cgggttggcg
     1381 ggaatgaaga tcgcacaccg gcaacgctgc acttcggcgt tcgacagacc ggcggtctcg
     1441 ttgccgaaca acagcgccac ctcgcccgtg ctggccttcg ccagaacgtc agcggcggcc
     1501 tgacgtgcct gcagcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     1621 gaagaatgag accttgcagg ccggttcata ttagaatcgg gactcctcca caccaatcgg
     1681 gcgacaaccg ccgcccgtca ggttctccaa aaccatgcat ccagctctca acatcatcat
     1741 caaggcggcg cggcgcgcca gtcagatcat caaccgcgcc tctctggatc tggagcacct
     1801 gcagatcacc accaagcgcc agagcgattt tgtcaccgaa gtggacaggg cggccgaagc
     1861 ggcaatcatc gacgtgctgc gcgaggccta tcccgagtac ggcattctag cagaagagtc
     1921 gggccagacc gccggcagcg ccggcggcag cgagtatcag tggatcatcg acccgctcga
     1981 cggcaccacc aacttcatcc acggattgcc gcagtacgcg gtctcgatcg cgctggcgca
     2041 ccgcggacag gtcacgcaag cggtggtcta cgacacggta cgcaacgaaa tgttcaccgc
     2101 cagcaagggc ggcggcgcct tcctcaacga acgccgcatc cgggtcagca aatgcctgcg
     2161 cctcgaagaa gcgctgatcg gcaccggttt tccctaccgc atttatgacc acatcgacgc
     2221 ctatctggcg atcttcaagg aactggcgcg caagagcgcc ggcatccgcc gcccgggtgc
     2281 cgccgccctc gatctggcct acgtcgcctg cgggcgcttc gacggcttct gggagttcgg
     2341 cctgtcgccc tgggacatcg ccggtggcgc gctgctgatt tccgaggccg gcggcctgat
     2401 cagcgacctt gccggcaacg aaacctacct gcagagcggc aacctggtcg ccggcaaccc
     2461 gaaagtcttt gcgcaactgc tgcaggtgat cgacggccac cggacgccgg ggttgatggc
     2521 gtaaaggcgt agggcggaag ctatcctgcc cggtgtgcgt tgctatcggc gtacgcaggg
     2581 caccggctcc caagcaaatt cgcatcaagg tggacgacgc actgtccaac gcggcagcct
     2641 taccttaacg tcgtcaccaa ggccgaccgc gtacaccaca gccatcaatt atggccaggc
     2701 gaaagcccct ggatttgccg catctcgaat ggggcaacat ggtaatgaac cccaagccct
     2761 cgaacagaag ctgcggtact gccttgccca cggtcattct ggtgtgccta ctcgccgcgc
     2821 aagatgctga actcctgaaa caggcgaagc ggaagcggta cactcagctt ccaatagatg
     2881 gtcatcggcc ttgcgccttc ggcatgctct aaagtgactg ggccacatgc tcggtatgga
     2941 tttcccctgg cgcagcggac gaacagttga aaactccagc cgttactgga actgtcgagg
     3001 taacggcggc ccgatggcgt atccggagcc gcactattct gggattgcca atggaaacgc
     3061 tcggtgctga tggcgaaatc gtggtaagca atgcgctggt gatagccgac tcgcttgtcc
     3121 agggtcacga acagcaattc gatcttgtgc tcgtgcaaag ctagcacgcc tgcccgaaat
     3181 ggcgtacggc gctcggcagt cagccaattc acggcggtca ggatttcacg aataccatac
     3241 gccgcatgca aacaaagagg cgtgctttcc agccccggaa ccgcctgatg actgag
//
LOCUS       DAOSIT010000242         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25434_length_3284_cov_7.398885, whole genome shotgun sequence.
ACCESSION   DAOSIT010000242 DAOSIT010000000
VERSION     DAOSIT010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25434_length_3284_cov_7.398885"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1412)
                     /locus_tag="PLL14_06750"
     CDS             complement(<1..>1412)
                     /locus_tag="PLL14_06750"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG03661.1"
                     /translation="FNAGHYSEGETFARQLTERFPEHGFGWKALGALLKLQGRNTESL
                     VALRQAADLLPRDEQVQSTMGLALADLGKPEESEACHRRALRIKPNLAEAHYNLGNCL
                     CAQGRYKEAENCYRRAVSLKPSYSLAYCNLGNALMDQGCLAEAEISYRRALALQPDLA
                     EAHLALGRNLKAQDRPNDAEASYRRALAYRPDDAEAHNHLGETLRDLGRLTEAESALC
                     RALELQPDFAEAHYHLGNTLGDQGRMEEAETAWRRAVGCKPDFLFPLVALGSALRKQG
                     RLREAEASLRRALEYWPEVAKAHFNLGNILSDQERLSEAEASYRRALELMPDLTDALL
                     NLSTTLIALGRYAEAEAGFRQALTTKPESAHAHSNLLFCLTHDERVDAAALFAEHCRF
                     GQQFEAPLRAHWTPHSNSRDPERCLQIGFLSPDFRNHAVANFIEPVLAELKNFRQLSL
                     HAYYSHTVEDHVTLRLREHI"
     assembly_gap    1413..1512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1756..2880)
                     /locus_tag="PLL14_06755"
     CDS             complement(1756..2880)
                     /locus_tag="PLL14_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOG03662.1"
                     /translation="MIRADVANRLQPAADIALRPAPPAQEITDKLSGLVAGQRLLAEI
                     QSLMPNGSYRALINQRNVTLALPFAAKSGDTVELEVTESDGKLTLAIVATAGSESGKD
                     EGGSAATSLSRTGQLISNLLGGPRDGKGGPWALPLNGNQPIATSPPTDAQELLPLLKQ
                     AITTSGMFYESHQAEWVEGRYSKEQLLQEPQGKLAPQTPLPQLQQPSTIGDTIVISGS
                     PADTAPVAAKTPEANTGAAQPSQAVAAQAQSLVQQQLEAFATQNFSWQGQVWPGQQMQ
                     WEIETDNEHQQSSGDDDSQKWQTRLRLTLPRLGEVEARLHVQGQQLTLAMRATSAETR
                     ELLRGGTATLRSQLEQAGLDLASLGVASPQEKQADVQFAE"
     gene            complement(2885..3175)
                     /locus_tag="PLL14_06760"
     CDS             complement(2885..3175)
                     /locus_tag="PLL14_06760"
                     /inference="COORDINATES: protein motif:HMM:NF017237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HOG03663.1"
                     /translation="MISVLQALEELLALSESMLSAAGAEDWETLASLEAARRALTESL
                     PAGLTADLPAGLQARARVLIENCLRCDKNLRPLLDTRVSELQVVLRAAQPAI"
BASE COUNT          508 a          965 c         1031 g          680 t
ORIGIN      
        1 gaatgtgttc tcgcaggcgc agcgtcacgt ggtcctcgac ggtatggctg tagtaggcat
       61 gcagtgacaa ttgccggaag ttcttcagtt cggcgagaac cggttcgatg aaattggcca
      121 cggcgtggtt gcggaagtct ggcgaaagaa aaccgatctg caggcaacgc tcggggtcac
      181 gagaattgct gtgcggcgtc cagtgggcac gcagaggggc ttcgaactgc tggccaaacc
      241 gacaatgctc ggcaaacagt gccgctgcat caactctttc gtcatgggtc agacaaaaga
      301 gcagattgct gtgcgcgtgc gcactctcgg gtttggtcgt cagggcctgc cgaaaacctg
      361 cttcggcctc agcgtatcgg ccgagtgcga tgagggtggt ggagagattc agaagtgcat
      421 cggtcaggtc gggcataagc tccagcgcgc ggcggtagct ggcttcggct tcgctcagtc
      481 gttcctggtc actcagaatg ttgccaaggt tgaaatgcgc cttggcaact tctggccagt
      541 attccagcgc gcgccgcagg ctggcttcgg cttctctgag gcgtccctgc ttcctgagtg
      601 cggaacccaa ggcaacgagc gggaagagga agtccggttt gcagcctacc gcgcgacgcc
      661 acgcggtttc ggcttcttcc atccgcccct ggtcaccgag ggtgttgcca aggtggtaat
      721 gcgcctcggc aaaatccggt tgcagttcca gggcgcggca caaggcgctt tccgcctcgg
      781 tcagtctgcc gagatctctc agcgtttctc ccagatggtt atgtgcttcg gcatcatccg
      841 gtcggtaagc cagcgcccgt cggtagctgg cctcggcgtc gttggggcgg tcctgcgcct
      901 tgagatttct gcctagcgcg agatgcgctt cggcgaggtc tggttgcagt gccaaagctc
      961 gccggtagct gatttcggcc tcggcaaggc aaccctggtc catgagcgcg ttgcccagat
     1021 tgcagtaggc aagggaatac gatggcttca aggacaccgc tctgcggtaa cagttctcgg
     1081 cttccttgta ccgcccctgc gcacagaggc aattgccaag gttgtagtgc gcttcggcca
     1141 ggtttggctt gattctcagc gcccggcggt ggcaggcttc cgattcctcc ggtttgccga
     1201 ggtctgcaag tgccagaccc atggtgctct gcacctgctc gtctcgtggc agcaagtctg
     1261 ccgcctggcg cagagcgacc agcgactcgg tgttgcgtcc ctgcaatttg agcaacgccc
     1321 cgagcgcttt ccacccaaaa ccatgctccg gaaaacgctc ggtcagttgt cgggcgaaag
     1381 tttctccctc gctgtagtgc ccggcgttga aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nngcttcgat ggcggcaagg aaatagggca ggcttgcggc cggtcgcagc
     1561 cgccggatct ccagaagacc aagttgatag ttcgcctccg ggtgatcagg ccgtgcctgc
     1621 aggatggctt gacagagctg ctcggcttcg ctgaactgtc cggcatcgtg gcatgccagc
     1681 gccttggcca aggcctgtgc aagcaccgcg tcaagggcgg cggcagcgct gcgcccagta
     1741 actgtcggca cggagtcatt ctgcaaactg gacatccgcc tgtttttcct gtggtgatgc
     1801 gacccccagt gacgcgagat ccagcccggc ctgttcaagc tgactgcgca aggtcgccgt
     1861 tccaccgcgc agcaactcac gcgtttcagc gctggtggcg cgcatggcca aggtaagctg
     1921 ctgtccctgg acatgaagtc gggcttccac ttctccgagc cggggcagcg tcagccgcag
     1981 ccgtgtctgc catttctggc tatcgtcgtc accactgctc tgctggtgct cgttgtcggt
     2041 ttcgatttcc cactgcatct gttggccggg ccagacctgc ccttgccagg aaaaattctg
     2101 ggtcgcgaag gcctcaagtt gctgttgaac aagcgactgg gcctgtgcgg ccaccgcttg
     2161 tgatggctgc gccgcgccgg tgttcgcttc tggcgtcttg gcggccactg gcgcggtgtc
     2221 ggcgggcgag ccgctgatga ctatggtgtc gccgatggtg gacggttgct gcaactgcgg
     2281 cagcggcgtc tgtggtgcca gttttccttg cggctcctgc aagagctgct ccttgctgta
     2341 gcggccctca acccattcgg cctggtgcga ctcgtagaac atgccgctgg tcgtgatcgc
     2401 ctgtttgagc agcggcagca gttcctgggc gtcagtgggt gggctggtgg cgattggttg
     2461 attgccattc agtggcagcg cccagggtcc acctttgccg tcacgcggac cgcccaggag
     2521 attgctgatc aactgcccgg tacggctcag gctggtcgcg gcggatccac cttcgtcctt
     2581 gccgctctcc gatccggccg ttgcaacgat ggccagggtc agcttgccgt cactttcggt
     2641 gacttcgagt tcgaccgtgt cgccgctctt ggccgcaaag ggcagagcaa gcgtcacgtt
     2701 gcgctggttg atcagcgcgc ggtagctgcc gttgggcatc aatgactgaa tttccgccag
     2761 caaccgttgc ccggcgacca ggccggacag cttgtcggtg atctcctgtg ccggcggcgc
     2821 cggccgcaac gcgatgtcgg ccgccggctg caagcgattg gctacgtcgg cgcggatcat
     2881 cggttcagat ggctggctga gccgcgcgta gaacgacctg caactcgctc acgcgggtgt
     2941 ccagcagtgg ccgcaggttc ttgtcgcagc gcaggcaatt ctcgatcagg acgcgcgctc
     3001 gcgcctgtaa cccggcagga aggtcggcgg tcagcccggc gggcaggctt tcggtgagcg
     3061 cgcggcgtgc cgcttccagg ctggccagtg tttcccagtc ctcagcaccc gcggcgctga
     3121 gcatgctctc ggagagcgcc agcagttctt ccagtgcctg cagcacggaa atcatgcgac
     3181 cgcttgctgt accgcgttcg gtgaaatttc gcgccacgcg ctgtgaattt cttcaagcag
     3241 gcccgcgacc tcttccagcg catgcagatc gttgttcagg ttgg
//
LOCUS       DAOSIT010000243         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25658_length_3264_cov_9.119663, whole genome shotgun sequence.
ACCESSION   DAOSIT010000243 DAOSIT010000000
VERSION     DAOSIT010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25658_length_3264_cov_9.119663"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="hflC"
                     /locus_tag="PLL14_06765"
     CDS             complement(<1..838)
                     /gene="hflC"
                     /locus_tag="PLL14_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOG03664.1"
                     /translation="MRAGMNVFAAVFVAVLVVLGATIFTVDQRQYAIVFQLGEVRSVI
                     EEPGLYLKWPLIQNVRYFDKRILTLDSAEPERFITSEKKNVLVDSFTKWRIIDPKLYY
                     ISVAGDESRAKTRLTQTVNAGLREEFGKRTVHDVVSGERDKVMEQMREKADLDARKIG
                     VQIVDVRVKRVELPNDVSESVYRRMDAERKRVANELRSQGAADAEKIRADADKQREVI
                     VAEAYRDAQKMKGEGDARATAIYAQAFEGNAEFYAFYRSLEAYRGSFKGKTDVIVVEP
                     NSD"
     gene            complement(838..2091)
                     /gene="hflK"
                     /locus_tag="PLL14_06770"
     CDS             complement(838..2091)
                     /gene="hflK"
                     /locus_tag="PLL14_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOG03665.1"
                     /translation="MSLNDPQWGNRGNDDGGGRRPNQGPPDLEQLWRDLNRRLSSIFD
                     KKGGGGDRGGDRPPVQFNSRFLGGGIGLLLALVVVVWLASGFYIVDASQVGLVLQFGR
                     YKESTDSGLRWRLPYPIQAHELVNVSGVRTLEIGYRGSEKNKVLKEALMLTDDENIIN
                     IQFAVQYILKDPVEYIFNNRHPDDAVMQVAETAIRQIVGKNKMDFVLYEGREQIAVDA
                     SKLMQDILDRYKTGILISKVTMQNAQPPEQVQAAFDDAVKASQDRERQKNEGQAYAND
                     VIPKARGTAARLIEEAEGYKKRVIATSEGDASRFKQIYTEYAKAPEVTRSRMYLDTMQ
                     QIYSNTSKVLVDTKGQGNLLYLPLDKLLQAAGAVTAAPAAGSPEAPVATRPAAAISNE
                     VPPQVVEKNEARSRETLRQRDREAR"
     gene            complement(2112..>3264)
                     /gene="hflX"
                     /locus_tag="PLL14_06775"
     CDS             complement(2112..>3264)
                     /gene="hflX"
                     /locus_tag="PLL14_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG03666.1"
                     /translation="EERAVIVQLDFGCQDLNEQLEEVRLLTRSAGAEICAVVQGRRHS
                     PDAATFAGKGKVQEVAAEVAAHEADLVIFNHELSAGQERNLERALQCRVIDRTSLILD
                     IFAQRAQSSEGKLQVELAQLEHLATRLVRGWTHLERQKGGIGLRGPGETQLETDRRLL
                     GIRVKLLKARLSRLERQRGVQRKARGRGELLNVSLVGYTNAGKSTLFNALTHAGVFAA
                     DQLFATLDTTTRKLWLPDAGHIVLSDTVGFIRDLPHSLVAAFHATLEATAEADVLLHV
                     VDSASSARADQVADVNKVLAEIGAAAVPQLMVLNKLDLTGLPPAVERDEYGRISRVRL
                     SARCGDGLPLLREALAEMALAKTANLRERSPGAVDLPDNDMVFDSDCSP"
BASE COUNT          595 a         1081 c          940 g          648 t
ORIGIN      
        1 agtccgaatt gggttcgacg acgatcacgt cggtcttgcc cttgaagctg ccgcggtagg
       61 cctccaggct acgatagaag gcatagaatt cggcattgcc ttcgaaggcc tgggcgtaaa
      121 tggcggtggc cctggcgtcg ccctcgccct tcatcttctg tgcgtcgcga taggcctcgg
      181 cgacgatcac ctcacgctgc ttgtcggcgt cggcgcgaat cttctcggca tcggcagcgc
      241 cctgcgagcg cagttcgttg gccacccgct tgcgttccgc gtccatgcgg cgatacaccg
      301 actcactgac gtcattaggc agttcgacgc gcttgacccg cacgtcgacg atctgcacgc
      361 cgatcttgcg cgcgtcaagg tcagccttct cgcgcatctg ctccatcacc ttgtcacgtt
      421 ctcccgagac cacatcgtgc acggtgcgct taccgaactc ctcacgcagg ccggcattca
      481 cggtctgcgt gagacgcgtc ttggcacgcg attcgtcgcc agccaccgag atgtaataca
      541 gcttcggatc aatgatccgc cacttggtga atgagtcgac cagcacgttc ttcttctcgg
      601 aagtgatgaa gcgttccggc tcggcactat cgagggtcag aatccgcttg tcgaaatagc
      661 gaacgttctg gatcagcggc cacttgaggt acaggcccgg ctcctcgatc acactgcgca
      721 cctcgccgag ctggaagacg atcgcgtatt gacgctgatc aaccgtaaag atcgttgccc
      781 caagcaccac cagcacggcc acaaaaaccg cggcaaagac attcatcccc gctctcatca
      841 gcgtgcctcc cgatcacgct gtcgcagcgt ctcgcgcgaa cgcgcctcgt tcttctcgac
      901 cacctgcggc ggcacctcgt tcgagatcgc cgctgccggg cgggtggcga ccggcgcctc
      961 ggggctaccg gcagccggtg ccgccgttac tgcaccagcg gcctgcaaca gcttgtccag
     1021 cggcagatag agcaggttcc cctgcccctt ggtatcgacc agcaccttgc tggtattcga
     1081 atagatctgc tgcatggtgt cgagatacat gcgactgcgc gttacttcgg gcgccttggc
     1141 gtattcggta tagatctgct tgaaacgact ggcgtcaccc tcggaagtgg cgatcactcg
     1201 tttcttgtag ccctcggcct cctcgatcag tcgcgcggcc gtgccgcgtg ctttcgggat
     1261 cacatcattg gcgtacgcct gcccctcgtt cttctggcgc tcgcgatcct gcgaggcctt
     1321 gacggcgtcg tcaaacgctg cctgcacctg ttccggcggc tgcgcgttct gcatcgtgac
     1381 cttggagatc aggatgccgg tcttgtaacg atcgagaata tcctgcatca gcttcgaggc
     1441 atcgaccgcg atctgctccc gaccttcgta gaggacgaag tccatcttgt tcttgccgac
     1501 gatctggcga atcgccgtct cggcgacctg catcacggcg tcgtcgggat gccggttatt
     1561 gaagatgtat tccaccggat ccttgaggat gtactgcacg gcaaactgga tgttgatgat
     1621 gttctcgtca tcagtcagca tcagcgcttc cttgaggacc ttgttcttct cgctgccgcg
     1681 ataaccgatc tccagggtac ggacacccga gacattgacc agttcgtgag cctggatcgg
     1741 gtacggcagt cgccagcgca aacccgagtc ggtgctctcc ttgtagcgcc cgaactggag
     1801 cacgaggccc acctgcgaag cgtcgacgat atagaaaccg ctggccagcc agaccacgac
     1861 gaccagcgcc agcaacaggc caatgccgcc accgagaaac ctgctgttga actgtaccgg
     1921 cggccggtcg ccgccgcggt cgccgccgcc acccttcttg tcgaaaatgc tggagagtcg
     1981 acgattgaga tcgcgccaca actgttcgag gtcaggcggc ccctgattgg gccgtctgcc
     2041 accgccgtca tcgttgccgc gattgcccca ttgggggtca ttgagcgaca taagtactcc
     2101 aaggcttgaa atcatgggga gcaatctgaa tcaaaaacca tatcgttatc cggcaagtcc
     2161 accgcgcccg gcgagcgttc gcgcaggttg gctgtcttcg ccagagccat ctcggccagt
     2221 gcctcgcgca gcaggggcaa accatcacca cacctggcgc tcaggcgaac gcgcgagatt
     2281 ctaccatact cgtcccgctc gaccgccggc ggcagaccgg taagatcaag cttgttgagg
     2341 accataagct gcggaaccgc tgccgcgccg atctcggcca gtaccttgtt gacgtcggca
     2401 acctgatccg cacgtgccga actcgccgaa tcgacgacgt gcagcagcac gtccgcctcg
     2461 gccgtggctt cgagcgtggc atggaaggcg gccaccagcg aatgcggcag atcgcggatg
     2521 aaacccacgg tatcggaaag cacgatatgg ccggcatccg gcagccacaa cttgcgcgtg
     2581 gtggtatcca gggtcgcaaa cagctggtcg gcagcaaaaa cgccggcatg ggtcaacgca
     2641 ttgaagagtg tcgacttgcc ggcattggtg taacccacca gcgaaacgtt caacaactcc
     2701 ccgcgaccac gcgccttgcg ctgcacgccg cgctggcgtt cgagtcggct caggcgcgct
     2761 ttcagcaatt tgacgcgaat acccagcagg cggcggtcgg tctcgagctg tgtctcaccg
     2821 ggccctcgca gaccgatacc gcccttctgc cgctcgaggt gcgtccagcc gcgaaccaac
     2881 cgcgtggcca ggtgttcgag ttgcgccagt tcgacctgca gcttgccctc ggaactctgc
     2941 gcccgctgcg caaagatgtc gaggatcaga ctggttcggt ctatcacccg acactgcaat
     3001 gcccgctcca gattgcgctc ctggccggcg ctcaattcgt ggttgaagat caccagatcc
     3061 gcctcgtgcg cagcgacctc ggcggccact tcctgcacct tgcctttgcc ggcgaacgtc
     3121 gcggcatccg ggctgtggcg gcgcccctgg accacggcgc agatttccgc tccagccgat
     3181 ctggtcagca gacgcacctc ttcaagctgc tcgttgagat cctggcatcc gaagtcgagc
     3241 tgcacgatca cggcgcgctc ctcg
//
LOCUS       DAOSIT010000244         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25717_length_3259_cov_12.035893, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000244 DAOSIT010000000
VERSION     DAOSIT010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_3259_cov_12.035893"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLL14_06780"
     CDS             complement(<1..233)
                     /locus_tag="PLL14_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290168.1"
                     /note="regulator of RNase E; increases half-life and
                     abundance of RNAs; interacts with RNase E possibly
                     inhibiting catalytic activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 4-hydroxy-4-methyl-2-oxoglutarate
                     aldolase"
                     /protein_id="HOG03667.1"
                     /translation="MSFKTPDLLDRHEEKTQNGSVRVVAPMFQPYGGRVSFSGQIVTL
                     KLFEDNSLVREALSGEGKGKVLVVDGGGSLRCAL"
     gene            complement(307..>1545)
                     /locus_tag="PLL14_06785"
     CDS             complement(307..>1545)
                     /locus_tag="PLL14_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG03668.1"
                     /translation="QKIDVSEPSSAEAVEILKGLKSRFESHHGIKYSAAALTSAVELS
                     VRFITDRHLPDKAIDVIDEAGAAQRILPKSRQKKLIGKIDIEEIVAKIARIPSTHVSS
                     DDRNNLRNLDRDLKAVVFGQEAAIDALARAIKMARSGLGNPSKPIGSFLFSGPTGVGK
                     TEVAKQLAYCLGNELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTRKPYS
                     VLLLDEIEKAHPDIYNILLQVMDHGTLTDNNGRKADFRNVVIIMTTNAGQEAIQKSTM
                     GFTPSRQAGDEMVEIKRIFTPEFRNRLDATISFKALDREVVLRVVDKFLMQLEAQLHE
                     KKVEAVFTRALKEHLAAHGFDPLMGARPMARLIQDTIRASLADELLFGRLASGGHVTV
                     DIDANQKVKLTFEEEPAIVA"
     assembly_gap    1546..1645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1646..2439)
                     /locus_tag="PLL14_06790"
     CDS             complement(<1646..2439)
                     /locus_tag="PLL14_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOG03669.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPSAAEAL
                     RACGANIEQLRKDLTRFIGEHTPTVAGDDEIDTQPTLGFQRVIQRAILHVQSSGRKEV
                     NGANVLVAIFGEKDSHAVYYLQKQGVTRLDVVNFIAHGISKVPQNAPRSDPEVETESE
                     PQVAAGPLESYTINLNALALQGKIDPLIGRDRELERVIQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIVECDIPEILAKANVYCLDMGSLLAGTKYRGDFEQR"
     gene            complement(2440..2748)
                     /gene="clpS"
                     /locus_tag="PLL14_06795"
     CDS             complement(2440..2748)
                     /gene="clpS"
                     /locus_tag="PLL14_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOG03670.1"
                     /translation="MATKHQDGSLLEARRAQLAPPPLYKVLLLNDDFTPMDFVIVVLQ
                     KFFGLDREQATRIMLKVHTEGRGICGIYSRDVASTKVEQVTTFARSNQHPLACVMEEN
                     "
     gene            3010..3213
                     /locus_tag="PLL14_06800"
     CDS             3010..3213
                     /locus_tag="PLL14_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG03671.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGSEDLFAHFSAITMGGFKTLKE
                     GEKVSFDVTQGPKGKQASNIARA"
BASE COUNT          601 a          900 c          939 g          719 t
ORIGIN      
        1 agtgcgcaac gcagcgaacc accgccgtca acaaccagca ccttgccctt gccctctccg
       61 gacagggctt ctcgcaccag cgagttgtcc tcgaacagct tgagcgtgac gatctggcca
      121 ctgaaagaaa cccgcccgcc gtaaggctga aacattggcg ccaccacacg gacgctaccg
      181 ttctgggtct tttcttcgtg cctgtcgagg aggtcgggtg tcttgaagct catgctgcct
      241 gccttgtggg tggttgaggg gaaccaaaat ccttacgggc tggttggcca gggaggtcgt
      301 cggcggtcag gctacgatcg ccggctcttc ttcgaaagtc agtttgacct tctggtttgc
      361 gtcgatgtcc acggtaacgt ggccgccact ggcaagcctg ccgaacagca gttcgtcggc
      421 cagagaggcg cggatcgtgt cctgaatcag gcgcgccatc gggcgggcgc ccattagcgg
      481 gtcgaagcca tgagcagcga gatgctcctt caatgccctg gtgaacaccg cttcgacttt
      541 tttctcgtgc aattgcgctt cgagttgcat caggaacttg tctaccacac gcaggaccac
      601 ctcgcggtcg agcgccttga acgagatcgt ggcgtccagg cgattgcgga actcaggggt
      661 gaagatgcgc ttgatctcga ccatctcgtc gccggcctgt ctcgatgggg tgaagcccat
      721 ggtggacttc tggatcgctt cctgcccggc gttggtggtc atgatgatca ccacattccg
      781 gaagtcggcc ttgcgcccgt tgttgtcggt cagcgtgcca tgatccatca cttgcaggag
      841 aatattgtag atatcgggat gcgccttctc gatctcgtcg agcaacagca cgctgtacgg
      901 cttccgggtg acggcctcgg tcagcaggcc accctggtcg aacccgacgt agcccggcgg
      961 agcaccaatc aggcgagaca ctgcgtgccg ttccatgtat tccgacatat cgaaacggat
     1021 cagttcgttc cccaggcagt aggccaattg cttggcgact tcggtcttgc cgacgccggt
     1081 gggacctgaa aacaggaagc tgccaatcgg cttactggga ttgcccagtc cggaacgggc
     1141 catcttgatc gccctggcga gcgcgtcgat ggctgcctcc tggccaaaca cgaccgcttt
     1201 cagatctcgg tcgagattcc gcaggttgtt cctgtcgtcg gaggaaacgt gcgtcgaagg
     1261 aatgcgcgcg atcttggcga cgatctcctc gatgtcgatc ttgccgatca gctttttctg
     1321 cctggacttc ggcaggatcc gctgcgcggc gcccgcttcg tcgatcacat cgatcgcctt
     1381 gtccggaagg tggcggtcgg taataaaacg caccgacaac tcgaccgccg aggtcagggc
     1441 agcggcagag tacttgattc cgtggtgaga ttcgaaccgc gacttgagtc ccttgaggat
     1501 ctcgaccgct tcggcgctcg acggctcgct gacgtcgatc ttctgnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnncgctg ttcaaagtcg ccacgatact tggtgccggc
     1681 cagcaacgag cccatgtcga gacagtacac gttggctttg gcaagaattt cggggatgtc
     1741 gcactcgacg atcctgcgcg ccaatccttc tgctatggcc gtcttgccga cgccagcctc
     1801 gccaaccagc aaggggttgt tcttccgccg acggcatagc gtctgaatga cgcgctcaag
     1861 ctcgcggtca cgtccgatca acgggtcgat cttgccctgg agggcaaggg cgttgaggtt
     1921 gatcgtgtaa ctctcgagcg ggccggccgc cacttgtggc tcgctttcag tctcgacttc
     1981 aggatcgctg cgtggcgcgt tctgggggac cttgctgatt ccgtgggcga tgaagttgac
     2041 gacatccaaa cgcgtgacac cctgcttttg cagatagtag accgcgtgcg aatccttctc
     2101 tccgaagatc gccaccagga cattggcccc gttgacttcc tttctgcccg atgactgaac
     2161 gtgcaggatc gcacgttgaa tcacgcgctg gaaacccagc gtcggctggg tatcgatctc
     2221 gtcatcgccg gcgacggtcg gagtgtgttc gccgataaag cgggtcagat ccttgcgcag
     2281 ttgttcgata ttggcgccac aggcgcgcag ggcttccgct gccgacggat tgtcgagcag
     2341 ggcaagcagg agatgctcca cggtaatgaa ctcatgacgt ttctgccgcg cttcgacaaa
     2401 tgccatatgc aagctgactt cgagttcctg cgcaatcatt cagttttcct ccattacgca
     2461 cgccagggga tgctggttgc tgcgtgcgaa ggttgtgacc tgctcaactt ttgtggacgc
     2521 gacatcgcgc gagtagattc cacagattcc ccgcccttcg gtgtggactt tgagcatgat
     2581 ccgcgtcgct tgttctctat ccaggccaaa aaacttttgc agaacgacga tcacgaaatc
     2641 catcggtgtg aagtcgtcgt tcagcagcag gaccttgtac agtggtggcg gcgcgagttg
     2701 cgcgcgtctt gcttcgagca gtgaaccgtc ttgatgcttt gttgccatgg atgaattcta
     2761 acgagtcttt gcggatgcgc aataccgctg ttggccaatc ttggcgcaat tgagcagcct
     2821 ggggagtcgc aaacgcggaa aacagttgac gcgtacgcct gacttggcgt aaaaaggggg
     2881 catgttttta gtcaagccga tgggtcgtgg ttccgcgtgt gtcaaatccc gctccggccg
     2941 ttgttgagtt aaaagcatgc aagtcgggaa atatgcccgg atttagtctt ttttcaagga
     3001 agtggcaata tggcaactgg tacagtaaag tggttcaatg acgccaaagg tttcggcttc
     3061 attactccgg acgatggcag cgaagatctt ttcgctcatt tctccgcgat caccatgggt
     3121 ggattcaaga ccctcaaaga gggcgagaag gtttccttcg acgtgacgca gggccccaag
     3181 ggcaagcaag catcgaatat cgcgcgcgct tgatcgcgtc aggtgtgcaa cagggacccg
     3241 cctccggcgg gtttttttt
//
LOCUS       DAOSIT010000245         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25718_length_3259_cov_11.432272, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000245 DAOSIT010000000
VERSION     DAOSIT010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25718_length_3259_cov_11.432272"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..220
                     /locus_tag="PLL14_06805"
     CDS             23..220
                     /locus_tag="PLL14_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOG03672.1"
                     /translation="MPQNTQQKPIEVSAHDLPLHCPTKDSPLWARHPRVFLDVLHTGE
                     AVCPYCSAHYVLTGERPKGHH"
     gene            259..1284
                     /gene="waaF"
                     /locus_tag="PLL14_06810"
     CDS             259..1284
                     /gene="waaF"
                     /locus_tag="PLL14_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOG03673.1"
                     /translation="MKALLVAPSWIGDAVLAQALCMRLHERNPGLQLDALAPRWVAPV
                     LERMPEIARVFDSPFAHGELSLKARHRLARRLAREDYQRAYILPNSIKSALIPFFADI
                     PERIGFVGETRYGLINRRHTLNKALLPQMAERFAQLAEPPGTALPRPLPQPRLCSTGE
                     QRAATLAALGIEIPERLVVFCPGAEYGPAKRWPERHFAALANALADHGYAVWLLGSAK
                     DRAIGEDIVSRTDAARLPLNLCGATSLSQAIDLLAAASFVVCNDSGLMHVAAALDRPL
                     IALYGSSSPGFTPPLSPRARILSLHLECSPCFKRECPLGHLDCLNRLEPQRVLDACLR
                     FPGQVSG"
     gene            1281..1730
                     /locus_tag="PLL14_06815"
     CDS             1281..1730
                     /locus_tag="PLL14_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOG03674.1"
                     /translation="MIAVFATPEDVETAFYEAIARADLAALMSVWADDEEIVCIHPTG
                     QRLTGTDAIRDSWRGIFANNPRLMVRLSRGVRWSGMLLAVHNVVETLYIGDERRPHGP
                     MLATNVFQRGASGWRLLSHHSSTAADRDAADTGVAGERNANTPRTLH"
     gene            1727..2698
                     /locus_tag="PLL14_06820"
     CDS             1727..2698
                     /locus_tag="PLL14_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOG03675.1"
                     /translation="MTTYRAPGWLPGGHAQTIYPLLIRPDPLPYHRDRWETPDGDFID
                     LDWNVVPPASNGADTRPLLVLFHGLEGSSVSHYAITLMRAATAIGWSGVVVNFRGCSG
                     ESNRLPRAYHSGDSDEIDWILRRLRALCPTRPCYAVGVSLGGNALLKWLGERQAGAGD
                     CLRAAAAISAPLDLTACGHHLARGFNRIYTQYFLRTLKRNATEKLRRYPGLFDERRMS
                     AANSLYEFDDVVTAPLHGFAGADDYWRKASSKPWLTGIRLPTLVLNAQNDPFLPAQAL
                     PLARQAAASVRLEFPRQGGHVGFVTGSLPGRLDWLPQRLLHFFQHEV"
     gene            2700..>3259
                     /locus_tag="PLL14_06825"
     CDS             2700..>3259
                     /locus_tag="PLL14_06825"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924611.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOG03676.1"
                     /translation="MNALLPTEIFKAYDIRGIVGKTLTVDIVRRIGHGLGSLAVERGQ
                     QAIAVGRDGRLSGPELSAALMEGIRLAGIDTIDVGCVPTPTVYFAAHELGCQSCVAVT
                     GSHNPPDYNGLKMVIGGETLAGAAIQSIRQRVDGGALHHGAGQGSSADVRSAYLRRIT
                     ADVTLARPMKIVIDCGNGVAGGIAPEL"
BASE COUNT          532 a         1186 c         1002 g          539 t
ORIGIN      
        1 gtccgtttgc aaggttgcca ccatgcctca gaacacccag cagaagccca tcgaggtcag
       61 cgctcacgac ctgccactgc actgcccgac caaggattca ccgctgtggg ccaggcatcc
      121 gcgagtcttt ctcgatgtcc tgcacacggg tgaagccgtt tgcccgtact gcagcgcgca
      181 ctacgtgctc accggcgaac gtccgaaagg acaccactga gcgctacccg gcccgcgtag
      241 ccggctgaac cgccaatcat gaaagcactc ctcgtcgcac cgtcctggat cggcgacgcg
      301 gtgttggcgc aagcgctgtg catgcggctg cacgaacgca accccggtct gcagctcgac
      361 gcgctggccc cgcgctgggt ggcaccggtt cttgagcgta tgccggagat cgcccgagtc
      421 ttcgacagcc cctttgcaca cggtgagctg tcgctgaaag cacgccaccg cctggctcgc
      481 cgactggcac gggaagacta ccagcgcgcc tacatcctgc ccaattcgat caagtcggcg
      541 ctgattccgt tcttcgccga catccccgag cgcatcggct tcgtcggcga aacccgctat
      601 ggactgatca accgccggca cacgctgaac aaggcactgc tgccgcaaat ggccgagcgt
      661 ttcgcacaac ttgccgagcc accgggtacc gcgctgccca gacccctgcc gcagccacgc
      721 ctatgctcga ccggcgagca acgcgcggcc acgctggcag cgctgggcat cgagatcccc
      781 gagagactgg tggtgttctg tcccggtgcc gaatacgggc cggccaagcg ctggccggaa
      841 cggcatttcg cggcgctggc caatgccctg gccgaccacg gctacgccgt ctggctgctg
      901 ggctcggcca aggaccgggc catcggcgag gacatcgtca gccgcaccga cgccgccagg
      961 ctgccgctca acctctgcgg cgccacctca ctgagccagg cgatcgacct gttggccgcc
     1021 gcatccttcg tcgtctgcaa cgattcaggc ctgatgcacg tcgccgccgc cctcgaccgg
     1081 ccgctgattg cgctctatgg ctcgtcgtca cccggcttca cgccgccgct gtcgccacgg
     1141 gcccggatcc tcagtctgca tctcgaatgc agcccgtgct tcaagcgcga atgcccgctg
     1201 ggtcatctcg actgcctcaa cagactcgaa ccgcaacgcg ttctcgacgc ctgcctgcgc
     1261 tttcccggtc aggtttccgg atgattgccg tttttgccac cccggaggat gtcgagacgg
     1321 ccttctacga ggccatcgcg cgggccgacc tggccgccct gatgagcgtc tgggccgacg
     1381 acgaagagat cgtctgcatc cacccgaccg gccagcgcct gaccggcacc gacgccatcc
     1441 gcgatagctg gcgcggcatc ttcgccaaca atccccgtct gatggttcgc ctgtcgcgcg
     1501 gggtacgctg gagcggcatg ctgctcgcgg tgcacaacgt cgtcgaaacg ctgtacatcg
     1561 gcgacgagcg ccggccgcac ggcccgatgc tggcgaccaa cgtcttccag cgcggcgcca
     1621 gtggctggcg cctgctctcg catcactcgt cgacagccgc cgatcgggac gcggccgaca
     1681 ccggagtagc cggcgaacgc aacgcgaata cgccgcggac gctgcattga ccacctaccg
     1741 cgcacctggc tggctgcccg gcgggcacgc gcagaccatc tacccgctgc tgatcaggcc
     1801 cgaccccctg ccctaccacc gcgaccgctg ggaaacgccg gatggcgact tcatcgacct
     1861 ggactggaat gtcgtaccac cggcgtcgaa cggcgccgac acacggccgc tgctggtcct
     1921 ttttcatggt ctggaaggca gttcggtcag ccactacgcg attactctga tgcgcgccgc
     1981 cacagccatc ggctggtctg gcgtggtggt gaattttcgc ggttgttcgg gcgagagcaa
     2041 tcgcctgccg cgcgcctatc attccggaga cagcgacgaa atcgactgga tcctgcggcg
     2101 cctgcgggcg ctttgcccga cgcgcccgtg ttacgccgtc ggcgtctcgc tgggcggcaa
     2161 tgccctgctc aaatggctgg gcgaaaggca ggcaggcgcc ggcgactgcc tccgcgccgc
     2221 ggcggcgatc agcgccccgc tcgacctgac cgcctgtggt caccacctcg cacgcggttt
     2281 caaccgcatc tacacgcagt acttcctgcg cacactcaag cgcaacgcca ccgagaagtt
     2341 gcgccgttat cccggcctgt tcgacgaacg gcgcatgtcc gcggccaaca gcctgtacga
     2401 gttcgacgac gtcgtcaccg ctccgctgca cggctttgcc ggcgccgacg actactggcg
     2461 caaagcgtcg agcaagccct ggctgaccgg catccgcttg ccgacgctgg tgctcaacgc
     2521 ccagaacgac cccttcctgc cggcccaggc gctgcctctg gctcgccagg ccgcggccag
     2581 cgtacgcctc gaattcccgc gccagggcgg gcacgtcggt ttcgtcaccg gcagcctgcc
     2641 ggggcgactc gactggctgc cacagcgcct tcttcatttt tttcagcacg aggtttgaca
     2701 tgaacgctct cctgcccacg gaaatcttca aggcctacga cattcgcggc atcgtcggca
     2761 agacgctgac cgtcgacatc gtccggcgca tcggccacgg cctggggtcg ctggcggtgg
     2821 agcgtggcca gcaggcgatc gccgtcggcc gcgacggacg cctgtcggga ccggaactgt
     2881 cggcggccct gatggagggc attcgcctgg ccggcatcga caccatcgat gtcggctgcg
     2941 tcccgacacc gacggtctac tttgcggcgc atgaactcgg ttgccagagc tgcgttgccg
     3001 tcaccggcag ccacaatccg ccggattaca acggcctgaa aatggtcatc ggcggcgaaa
     3061 ccctggccgg tgcagcgatt cagagtatcc ggcagcgggt tgacggcggt gccttgcacc
     3121 acggtgccgg gcaaggcagc agcgccgacg tgcgctcggc gtaccttcgc cgcatcaccg
     3181 ctgacgtcac gctggcgcgg ccgatgaaga tcgtcatcga ctgcggcaac ggcgtcgccg
     3241 gcggcatcgc cccggaact
//
LOCUS       DAOSIT010000246         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25913_length_3239_cov_6.937186, whole genome shotgun sequence.
ACCESSION   DAOSIT010000246 DAOSIT010000000
VERSION     DAOSIT010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25913_length_3239_cov_6.937186"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..248
                     /locus_tag="PLL14_06830"
     CDS             18..248
                     /locus_tag="PLL14_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster co-chaperone HscB C-terminal
                     domain-containing protein"
                     /protein_id="HOG03677.1"
                     /translation="MEWREAVAEARAAGDHHELERLHHRLQREMRERHEEIAVLLDDR
                     PDLEQAADRVRRLMFLEKLLADIDDAIAACEE"
     gene            419..2320
                     /gene="hscA"
                     /locus_tag="PLL14_06835"
     CDS             419..2320
                     /gene="hscA"
                     /locus_tag="PLL14_06835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOG03678.1"
                     /translation="MALLQIAEPGESAAPHQHRLAVGIDLGTTNSLVATVRSGLAVVL
                     SDELGRPLLPSVVRYLPDGGSEVGYEAQARQSLDPRNSIVSVKRFMGRGLQDIAHREG
                     MPYVFDDAPGMLRLQTAAGRKSPVEVSAEILRTLRLRAEASLGGPLVGAVITVPAYFD
                     DAQRQATKDAARLAGLPVLRLLNEPTAAAIAYGLDNASEGIYAVYDLGGGTFDISILR
                     LARGIFEVLATGGDSALGGDDFDHRIFCWMLEAAQLKPLSVEDSRMLLTRAREAKEYL
                     TFHGVAPINARLNSGEAVDLTLTTEIFAEISQTLVAKTIQPVKKALRDAGLSVADVKG
                     VVMVGGATRMPQVQHAVGEFFRQEPLNNLDPDKVVAIGAAKQANLLVGNGLAGDDWLL
                     LDVIPLSLGLETMGGLVEKIIPRNATIPTARAQEFTTFRDGQTALAVHVVQGERELVS
                     DCRSLARFELRGIPPMVAGAARIRVTFQVDADGLLAVTAREQTAGVEASIVVKPSYGL
                     SDGEIAGMLRDSMLHVRDDALQRALKEAQVDAQRLIEAVSSALATDGDLLSGDELARV
                     EHGIVKLQGAASGENRRQIMLAMDDLEAETKDFAARRMDKSIRRAFAGRNISELDSEV
                     LATRAGETA"
     gene            2317..2658
                     /gene="fdx"
                     /locus_tag="PLL14_06840"
     CDS             2317..2658
                     /gene="fdx"
                     /locus_tag="PLL14_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOG03679.1"
                     /translation="MTQIIVLPHVELCPDGAVIDARPGESLCQNLLENGIAIEHACEM
                     SCACTTCHVIVREGFEALEPSDEVEDDLLDKAWGLEPNSRLSCQAMVNETPLLIEIPR
                     YSINMAREGKR"
     gene            2655..2849
                     /gene="iscX"
                     /locus_tag="PLL14_06845"
     CDS             2655..2849
                     /gene="iscX"
                     /locus_tag="PLL14_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HOG03680.1"
                     /translation="MKWTDINDLAVELAERHPDVDPMKINFVDLMHWVLALPGFEGDA
                     KHCGEKVLEAIQQAWIDELT"
     gene            2846..3235
                     /locus_tag="PLL14_06850"
     CDS             2846..3235
                     /locus_tag="PLL14_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HOG03681.1"
                     /translation="MKPDDCGGSQRNVLGGLLGSCSEAPITGFFRDGCCNTSDEDLGS
                     HTICVVLTADFLAFSKGRGNDLSTPRPEFGFPGLKPGDRWCLCAARWREALQAGKAPR
                     VVLNATNEAALLIVSLDDLKRHAIDLN"
BASE COUNT          572 a          995 c         1080 g          592 t
ORIGIN      
        1 acttcctcgt tgagcagatg gaatggcgcg aggcggttgc cgaagcgcgc gctgcgggcg
       61 atcatcatga gctggagcgt ctgcatcacc gcttgcaacg tgagatgcgt gagcgccatg
      121 aggaaatcgc cgtgctgctc gacgatcgtc ctgatctgga gcaggctgct gatcgcgtgc
      181 ggcgactgat gtttctggag aagttgctcg ctgacatcga tgatgcgatt gcggcgtgtg
      241 aagagtaggc cgtacacctg tatgcgattg gccagccgat ttccagcgga cagggaaccg
      301 ttgaccgttt gcctcacccc ccgcccttcg cccgcacgcg ggcgaagggg agcgccgtga
      361 gtcgctggcg cgacctccac gtcatccacg aaccaagcct caggacctca ttttcttcat
      421 ggctctatta cagattgcgg aaccgggcga atcggcagcg ccgcaccagc accggctggc
      481 tgtcggcatc gacctcggga cgaccaattc gctggtggcg acggtgcgca gcggtttggc
      541 cgttgtcctc agcgacgagt tgggccgccc cttgttgcct tcggtggtgc gttacctgcc
      601 ggacggtggc agcgaagtcg gttacgaagc gcaggccaga caatcgctcg atccgcgcaa
      661 cagcatcgtt tcggtcaagc gcttcatggg ccgtgggctg caggacatcg ctcaccgcga
      721 gggcatgccc tacgtcttcg acgatgcacc ggggatgctg cgcctgcaga ccgccgccgg
      781 tcggaagagt cctgtcgagg tctcggccga gatcctgcgc accctgcggt tgcgggccga
      841 ggcctcgctc ggtgggccgc tggtcggtgc ggtgatcacc gtcccggcct attttgacga
      901 cgcgcagcgc caggcgacca aggacgccgc caggctcgcc ggactgccgg ttctgcgcct
      961 gctcaacgag ccgacggcgg cggcgatcgc ctacgggctc gacaacgcct ccgaaggcat
     1021 ctatgcggtc tatgacctcg gcggcggcac cttcgacatc tcgattctcc gactcgcgcg
     1081 tggcatcttc gaggtgctgg ctacgggcgg cgactcggcg ctgggtggcg acgatttcga
     1141 ccatcgcatc ttctgctgga tgctcgaagc ggcccaactc aaacccttgt cggtcgagga
     1201 ttcgcggatg ctgctgacgc gcgcgcgcga ggccaaggaa tacctgacct ttcatggcgt
     1261 ggccccgatc aacgcccgtc tgaacagtgg cgaggcggtg gatctgacgc tgactacgga
     1321 gatctttgcc gagatcagcc agacgctggt ggcgaagacc attcagccgg tcaagaaggc
     1381 cctgcgcgat gccgggctgt cggtggccga cgtcaagggc gtggtcatgg tcggcggcgc
     1441 gacgcgcatg ccgcaggtgc aacacgcggt cggcgagttc ttccggcagg agccgctgaa
     1501 caatctcgat cccgacaagg tggtggccat tggtgcggcg aagcaggcca atctcctggt
     1561 tggcaacggg ctggccggcg acgactggct gttgctcgac gtgattccct tgtcgctggg
     1621 gctcgaaacg atgggaggtc tggttgaaaa gatcatcccg cgcaatgcca ccattccgac
     1681 ggcgcgggcg caggaattca cgacttttcg cgacggccag acggcgctgg ctgtacatgt
     1741 ggtgcagggc gagcgcgaac tggtcagcga ttgtcgttca ttggcccgtt ttgagctgcg
     1801 cggcattccg ccgatggtcg ccggcgccgc acgcatccgc gtcactttcc aggtcgatgc
     1861 cgacggtctg ctggcggtga cggcccgcga gcagaccgcc ggtgtcgaag cgagcatcgt
     1921 cgtcaagccg tcctacgggc tgtcggatgg cgagatcgct ggcatgctca gggactcgat
     1981 gctgcacgtc agggatgacg cgctacagcg cgcgctcaag gaagcgcagg tggacgcgca
     2041 gcggctgatc gaggcggtaa gttctgctct ggcgacggat ggcgacctgc tctccggcga
     2101 tgaactggcg cgggtagagc acggcatcgt caagcttcag ggtgcggcca gcggtgagaa
     2161 ccgtcggcag atcatgctgg cgatggacga cctcgaagcc gagaccaagg attttgcggc
     2221 caggcgcatg gacaagtcga tccggcgggc cttcgccggg cgcaacatca gcgaactgga
     2281 cagcgaagtg ctcgctacgc gggcggggga aacagcatga cccagatcat cgttctgccg
     2341 catgtcgaac tctgccccga tggcgcggtg atcgacgccc gtcccggtga atcgctgtgc
     2401 cagaatctgc tcgagaacgg tatcgccatc gaacatgcct gcgagatgtc ctgtgcctgc
     2461 accacctgcc acgtcatcgt tcgcgaaggc ttcgaggcgc tcgaaccgtc cgatgaggtc
     2521 gaggacgatc tgctcgacaa ggcctgggga ctggaaccca actcacgcct gtcgtgtcag
     2581 gcgatggtca atgaaacgcc gttgctgatc gagattccgc gctactcgat caacatggcc
     2641 agggagggca agcgatgaaa tggaccgaca tcaacgacct cgccgtcgaa cttgcggaga
     2701 ggcacccgga cgtggacccg atgaagatca acttcgtcga tctgatgcac tgggtcctgg
     2761 ccttgcccgg cttcgaaggc gacgcgaagc attgcggcga gaaggtgctg gaggcgatcc
     2821 agcaagcctg gattgatgaa ctgacatgaa acccgatgat tgcggcggca gccagcgcaa
     2881 cgtcctcggc ggtttgctcg gctcgtgcag cgaggcaccg attaccggct tctttcgcga
     2941 tggttgttgc aacaccagtg acgaagacct cggcagtcac accatttgcg tcgtgctcac
     3001 ggcagatttt cttgcgtttt cgaaagggcg cggcaacgac ctgtccacgc ccaggccgga
     3061 gttcggtttc cccggcctga agcccggcga tcgctggtgt ctgtgtgccg cccgctggcg
     3121 cgaagccctg caggctggca aggcgccgcg cgtcgtgctc aatgccacca acgaagccgc
     3181 tttgctgatc gtcagcctcg acgacctcaa gcgccacgcg atcgacctca actgacggg
//
LOCUS       DAOSIT010000247         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_25920_length_3238_cov_8.665724, whole genome shotgun sequence.
ACCESSION   DAOSIT010000247 DAOSIT010000000
VERSION     DAOSIT010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25920_length_3238_cov_8.665724"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="PLL14_06855"
     CDS             <1..1114
                     /locus_tag="PLL14_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOG03682.1"
                     /translation="ARLEEMLGTPARIYYKNEGVSPAGSHKPNTAVAQAYYNKQAGIK
                     RITTETGAGQWGCSMALAGQMFGLEVRVYMVRISYQQKPYRRSMMQTWGAEVFASPSA
                     MTQTGRAALEQDPENMGSLGLAISEAVEEAASRTDTNYALGSVLNHVALHQTIIGLEA
                     RKQFELAGDWPDVVFAPCGGGSSFAGIAFPFIADNAVGGHQGKPVRLVAVEPSSCPSL
                     TKGQYAYDFGDASGFTPLMKMYTLGHDFMPPGIHAGGLRYHGDSPLVSQLYHEGLIEA
                     VAVAQLATFEAGVMFARAEGVIPAPESNHAIRAAIDEALRCRTTGEARTIFFNLTGHG
                     HFDMAAYDRYFAGELEDFEYPAEAIRTSLEHLPKVG"
     gene            1111..1809
                     /locus_tag="PLL14_06860"
     CDS             1111..1809
                     /locus_tag="PLL14_06860"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG03683.1"
                     /translation="MIANMRIVVFLLVLANLLVLVWTRGYLGAPANPDARRAEQQLLP
                     EQVKVVGRAEPPQATNRKDEGEKVADAKGAVACQFWSDLVSADADQVERLLAEQFSAF
                     KATRRTIAENSGYWVYVPPLANKEEVNKKTAELQLLGIQDFFVVQASGPNQLAISLGT
                     YRTEEAAKAGLEGLRAKGVKSAKMSERKGKPPFNTLEIRGPATQVDALRQAIVTLLPK
                     ASPTACKVKAEAAP"
     gene            1806..2429
                     /gene="coaE"
                     /locus_tag="PLL14_06865"
     CDS             1806..2429
                     /gene="coaE"
                     /locus_tag="PLL14_06865"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765348.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG03684.1"
                     /translation="MSFVVGLTGGIGSGKSTVAELFVQHGAALVDTDAIAHTLTAPHG
                     AAMAAIRNAFGERVLRADGGLDRAAMRSLVFSDHTAKATLEAILHPLIREHSEARCAD
                     ATSAPYVLLVVPLLVESGSYRRRADRILVVDCDEAVQIARVMTRSGLTAGEVEAIMAT
                     QASRLERRAAADDVVLNDRELGALAPQVDGLHRQYLELAMAKAHAGR"
     gene            2490..>3238
                     /gene="zapD"
                     /locus_tag="PLL14_06870"
     CDS             2490..>3238
                     /gene="zapD"
                     /locus_tag="PLL14_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOG03685.1"
                     /translation="MITYEYPFNERIRTLLRLEDLFQKAGSFLQRDGSQEHHVVLLTL
                     FEILDVAGRADLKMDLIQELERQRQSLQLFRNNPDISEEALSGALYEIEHASAALLGM
                     TGKMGQHIRDNDWLMSIKSRASIPGGVCEFDLPSYHYWRHQAPGIRRDALLAWLQPML
                     PLRDALAIVLRLLRSSGRPEHHVATGGAFQLMLGGSSSQMVRISLKLHDPYIPEISAN
                     KYALNIRFTRPDSDHRPRHCDNDVEFDMLFC"
BASE COUNT          630 a         1008 c         1008 g          592 t
ORIGIN      
        1 ggcgcggctc gaggaaatgc tgggcacgcc ggcgagaatc tattacaaga atgaaggcgt
       61 ttcaccggcc ggttcgcaca agcccaacac cgccgtggcg caggcctatt acaacaagca
      121 ggcggggatc aaacgcatca ccaccgagac cggcgccggg caatggggtt gttcgatggc
      181 gctggccggg cagatgttcg ggctcgaagt gcgcgtctac atggtcagga tcagctacca
      241 gcagaagcct taccgccgct cgatgatgca gacctggggt gccgaggtgt tcgccagccc
      301 gtcggcgatg acgcagaccg gccgcgctgc gctcgaacaa gacccggaaa acatgggttc
      361 gctcggactg gcgatttccg aggctgtcga agaggcagca tcacgcaccg ataccaacta
      421 cgcgctcggc tcggtgctca atcacgttgc gctgcatcag acgatcatcg ggctggaggc
      481 caggaagcag ttcgaactcg ccggtgactg gcctgacgtc gtcttcgcgc cctgcggcgg
      541 tggttcctcg tttgccggta tcgcctttcc gttcattgcc gacaatgctg tcggtgggca
      601 tcaaggaaag ccagtgcggc tggtggcggt cgagccctcg tcctgcccgt cgctgaccaa
      661 gggccaatac gcctacgatt ttggcgatgc gtccggcttc acgccgctga tgaagatgta
      721 caccctcggc cacgacttca tgccgccggg catccatgcc ggtggcctgc gttatcatgg
      781 cgattcgcca ctggtttcgc agctctacca tgaaggcctg atcgaggctg ttgccgtggc
      841 gcaactggcg accttcgaag ccggcgtgat gttcgcccgc gccgagggcg tgattcccgc
      901 accggagtcc aaccatgcga tccgcgcggc catcgacgag gcgctgcgct gcaggacaac
      961 cggcgaggcg cggacgatct tcttcaatct caccgggcac ggacacttcg acatggctgc
     1021 ctacgaccgc tatttcgccg gtgaactcga ggatttcgag tacccggctg aagccatcag
     1081 gacgtcgctg gaacatctgc cgaaagtggg atgattgcca acatgcgtat cgtcgtgttc
     1141 ctgctggtgc tggccaacct gctggtgctg gtgtggacgc gcggctacct cggcgcgccg
     1201 gccaatcccg acgcgcggcg tgccgagcag cagttgcttc ccgaacaggt gaaggtcgtc
     1261 gggcgggcgg aaccaccgca ggcgacgaac aggaaagatg aaggcgaaaa agttgccgac
     1321 gcgaagggcg ccgtcgcctg ccagttctgg agcgatctgg tcagcgccga cgcggatcag
     1381 gtcgagcgcc ttctggctga acagttctcg gcgttcaagg caacgcggcg gacgatcgcc
     1441 gagaacagcg gctactgggt ctatgtcccg ccactggcca acaaggagga agtcaacaaa
     1501 aagaccgctg aattgcaact gctgggcatc caggatttct tcgtcgtgca ggccagcggg
     1561 cccaaccagc tggccatctc gctgggcacc taccgcaccg aggaagcggc caaggcaggg
     1621 ctggaaggct tgcgcgccaa gggcgtcaag tcggcaaaaa tgagcgagcg caagggtaag
     1681 ccaccgttca acaccctcga aatccgtggg cctgcaaccc aggtcgatgc tctgcgccag
     1741 gcgattgtca cactgctgcc gaaagccagc ccgaccgcct gcaaagtcaa ggccgaggcg
     1801 gcgccatgag cttcgtggtg gggctcaccg gcggcatcgg tagcggcaag agtacggttg
     1861 ccgaactgtt cgttcaacac ggcgccgccc tggtcgacac cgatgccatt gcgcataccc
     1921 tgaccgctcc acacggcgcg gccatggccg cgatcaggaa cgcctttggc gagcgtgtgc
     1981 tgcgggccga cggtggcctg gaccgcgccg cgatgcgcag cctggttttc tctgatcaca
     2041 cggcgaaggc gacactcgaa gccatcctgc atccactgat ccgagagcat agcgaggcgc
     2101 gttgcgcaga tgcgacaagt gctccgtacg tgttgctcgt ggtacctttg ctggtcgaat
     2161 cgggcagcta ccgccgccgc gccgaccgga tcctcgtcgt cgattgcgac gaggcggtac
     2221 agattgctcg cgtcatgacg cgcagcggac tcaccgccgg agaggtcgag gcgatcatgg
     2281 ccacgcaggc gtcacgactc gaaaggcgcg cggcggcaga cgatgtagtg ctcaatgatc
     2341 gcgaactcgg cgctctggca ccacaggtcg acggtctgca ccgccagtat cttgaactgg
     2401 ccatggccaa agctcatgct gggcgttgag gttttgctgc aaatgattca gaattatcga
     2461 aaatccccgt tcccggcgct ggcggcagtg tgatcacata cgaatatcct ttcaatgaac
     2521 gcatacgcac gctgttgcgt ctggaggatt tgttccagaa ggctggcagt ttcctgcagc
     2581 gggatggatc ccaggagcac catgttgtcc tgttgacgct tttcgaaatc ctcgatgtcg
     2641 cagggcgcgc cgacctgaaa atggatctga ttcaggaact cgaacgtcag cgccagagcc
     2701 tgcagctttt tcgcaacaat ccggatattt ccgaagaagc cctctccggc gccctgtacg
     2761 agatcgagca cgcctccgca gcgttgctgg gcatgaccgg aaaaatgggg cagcatattc
     2821 gtgacaacga ctggctgatg agcatcaaga gtcgcgccag cattcccggc ggcgtctgcg
     2881 agttcgacct gccgtcatac cattactggc gtcatcaggc ccccggcatt cgccgcgacg
     2941 ccctgctggc ctggctgcag cccatgctgc cgctgcgtga cgcactggcc atcgtactgc
     3001 gcctgttacg ttccagtggc cgtcccgagc accacgtggc taccggcggc gccttccagc
     3061 tcatgcttgg cggtagcagc tcacagatgg tgcgtatctc gctcaagctg cacgatccct
     3121 atattcctga aatcagcgcc aacaagtacg cgctgaacat ccgcttcaca cgacccgaca
     3181 gcgatcaccg gccgcggcat tgtgacaacg atgtcgagtt tgacatgctg ttctgcaa
//
LOCUS       DAOSIT010000248         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26063_length_3225_cov_7.331230, whole genome shotgun sequence.
ACCESSION   DAOSIT010000248 DAOSIT010000000
VERSION     DAOSIT010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26063_length_3225_cov_7.331230"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3225
                     /locus_tag="PLL14_06875"
     CDS             <1..>3225
                     /locus_tag="PLL14_06875"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG03686.1"
                     /translation="QFWEVLSSGQHCFEKIPSERWRHAGIYHDQRDILGKTTIRNGSF
                     LSDIDRFDPRYFNISQRDAELMSPEVRLFLQVGVEAFEDAGYSREEMHQRFDGDVGVL
                     VGTMSNHYGLYGFQNMLVRGARANGSYTGTLPNMLSYFYGFSGPSIFMDTMCSAAATC
                     IDQGVHMLRSRQCRMVLAGGANLLLHPYNFISSSQEHFTTRTSELIRSYGQGADGTIL
                     GEGVGAVLLKPLVEAIRDRDHIYGVIKGTAINNAGVRNGFTVPSPTMQARAVDKALRD
                     AAVDPRSISYVEGHGSGTKLGDPIEIKALTQAYRSYTEARQFCPIGSVKSNIAHLLAA
                     AGVAGFTKVLLQLKHQQIVPSLHSEELNPDIPFADSPFYVQRELSGWERPKVDGQVYP
                     RRAGVTSIGAGGMNSHIIVEEYSATMAPRLPSQPRLVVVSAGSRSLLLSYLERLRGYL
                     ERHPDSDLASLSYTLQVGRNALPCRLAVVVADAAALLRTIAGFARDGVADGLRHGDNI
                     VTGNQDFDDADIERALDTASLERIADYWVRGAAIDWDRLWGMQLPYRISLPTCPFEKI
                     RCWYAVDADAPTVENPLSSQRKCHPFIGRNVSDLASVRYETDFRISELLDYAFQHGTR
                     RQILPSFVIDTALALAGIAGVRGEFVVADLKWHQAADWEAIATLYIAIAAGSDGDHAA
                     QIAWQEPDDAPRLLAEWQLRPALLSPAPAPAPLPAAQQTIDGADFYRELQEGGLDYAP
                     YLEGVHHLSMLDGGACLATLQAPPLRQHHYPSNVRIEPYVLGALVQVAQYLAKREGRS
                     DWAGYAPLDIGELALLPGQVTQVLLHGLRGRAELLASDATILGRISGIRFGNAAADAT
                     PLAAVTVAVAVPDDESALIRADLVAQIRAHMSAILKFPLADIDTRTPFYNFGFDSISM
                     TELAGKISVEFGLSLTPALFFEVENADQLVEHMLGAHLLKLQERWRRTHQQQRPAAAP
                     PGAAAAALAGSRSLSRSPAGPGLPIAIIGAAGRFPGADDLDAFWDQLAGGCNAIGPLP
                     MSRYGEAYQELLRHADFPKRAGILHGIDRFDAAFFNISPTEAEL"
BASE COUNT          547 a         1106 c         1022 g          550 t
ORIGIN      
        1 agcagttctg ggaagtgctc agcagcggcc agcactgctt cgagaagatc ccgtccgagc
       61 gctggcggca cgccggcatc taccacgacc agcgtgacat cctcggcaag acgaccatcc
      121 gcaacggcag cttcctcagc gatatcgacc gcttcgatcc gcgctacttc aatatctcgc
      181 agcgcgacgc cgagctgatg tcacccgagg tgcgcctgtt cctgcaggtc ggggtcgaag
      241 ccttcgaaga cgccggctat tcgcgcgagg agatgcacca gcgcttcgac ggcgacgtcg
      301 gggtgctggt cgggacgatg agcaaccatt acggcctgta cggctttcag aacatgctgg
      361 tgcgtggtgc gcgtgccaac ggcagctaca ccggcaccct gccgaacatg ctgtcctatt
      421 tctacgggtt ttccggtcca tcgatcttca tggataccat gtgctcggcc gccgccacct
      481 gcatcgacca gggcgtgcac atgctgcgct cacggcagtg ccggatggtg ctggcgggcg
      541 gcgccaatct gctgctgcat ccctacaact tcatctccag ttcgcaggag cacttcacca
      601 cccgcacctc ggagctgatc cgcagctacg gccagggcgc cgacggcacc attcttggcg
      661 aaggtgtcgg cgcggtgctg ctcaagccgc tggtcgaggc gatcagggac cgcgaccaca
      721 tctacggcgt catcaagggc acggcgatca acaatgccgg cgtgcgcaac ggctttaccg
      781 tgcccagccc gacgatgcag gcccgggcgg tggacaaggc gctgcgcgat gcggccgtcg
      841 acccgcgcag catcagctat gtcgaagggc acggatcggg taccaagctc ggcgacccga
      901 tcgagatcaa ggccctgacc caggcctacc gttcgtatac cgaggcgcgc cagttctgcc
      961 cgatcggttc ggtgaaatcg aatatcgcgc acttgctggc cgccgccggg gtggccggat
     1021 tcaccaaggt gctgctgcag ctcaagcacc agcagatcgt gccctcgctg cattccgagg
     1081 aactcaaccc cgacatcccg ttcgccgatt cgccgttcta cgtgcagcgc gagctgtccg
     1141 gctgggagcg cccgaaggtc gatggccagg tttatccgcg tcgtgccggc gtcacttcca
     1201 tcggtgccgg cggcatgaat tcgcacatca tcgttgaaga gtacagcgca accatggcgc
     1261 cgcgcctgcc aagccagcca cgcctggtgg tggtgtcggc ggggagccga agcctgttgc
     1321 tgagctacct ggaacgtttg cgcggatacc tggaacggca tcccgacagc gacctggctt
     1381 cgctctcgta tacgctgcag gtgggccgca acgccttgcc gtgtcgcctt gccgtggtcg
     1441 tcgccgacgc cgcggcgctg ttgcgaacga tcgccggttt cgcgcgcgac ggtgtcgcgg
     1501 acgggctacg ccatggcgac aatatcgtca ctggcaatca ggacttcgac gacgccgaca
     1561 tcgagcgcgc gctcgacacc gccagcctgg agcgcatcgc cgactactgg gtacgcggtg
     1621 cggcgatcga ctgggaccgc ctgtggggca tgcaactgcc gtaccgcatc tcgctgccca
     1681 cctgcccctt tgaaaagatc cgttgctggt acgccgtcga tgcggacgca ccaacagtcg
     1741 aaaatcccct gtcttcgcaa cgcaagtgtc accccttcat cggccgcaac gtgtccgacc
     1801 tggcctcggt gcgctacgag accgactttc ggatcagtga actgctcgac tacgcttttc
     1861 aacacggcac gcgtcggcag atcctgccga gtttcgtgat cgacactgcg ctggccctgg
     1921 ccggcatcgc tggcgtcagg ggcgagttcg tggtggccga cctgaagtgg catcaggccg
     1981 ccgactggga ggcgatcgct accctgtata tagccattgc cgcaggctca gacggcgacc
     2041 atgcggcgca gatcgcctgg caggagccgg acgacgcgcc gcgcttgctg gccgaatggc
     2101 agttgcggcc ggcgctgctg tcgccggcgc cggcgccggc gccattgccg gcagcgcagc
     2161 aaaccattga cggcgccgac ttctaccggg agctccagga gggcggcctc gactacgcgc
     2221 cttatctcga aggtgtacac catctgtcga tgctcgacgg cggcgcctgc ctggccacgc
     2281 tgcaggcacc gccgctccgg cagcaccact acccgtcgaa tgtgcgcatc gagccgtacg
     2341 tgctcggcgc actggtgcag gtagcgcaat acctggccaa gcgcgaaggc cgcagcgact
     2401 gggccggtta tgcgccgctc gacatcggcg aactggcgct gctgcccggc caggtcacgc
     2461 aggtgctgct gcacggcctg cgtggccgtg ccgaattgct cgccagcgac gcgacaatcc
     2521 tgggccggat cagcggtatc cgcttcggca acgccgccgc cgacgccacg ccgctcgcgg
     2581 cggtgacggt ggcggtggcg gtgcctgacg atgaaagcgc actgatccgt gccgatctgg
     2641 tggcgcagat ccgcgcgcac atgtcggcga tcctgaaatt cccgctcgcc gacatcgaca
     2701 cccgcacccc cttctacaac ttcggcttcg attcgatctc gatgaccgaa ctggccggca
     2761 agatcagcgt cgagttcggg ctcagcctga ctccggcact gttcttcgag gtcgaaaacg
     2821 ccgatcagtt ggtcgagcac atgctgggcg cgcacctcct gaaactgcag gagcgctggc
     2881 gacgcaccca tcaacagcaa cggccagccg ccgcaccgcc aggcgccgcg gcggcggccc
     2941 tggcgggcag ccgctcgctg tcacgcagcc cggccgggcc gggtctgccg atcgcgatca
     3001 tcggtgcggc cggcaggttt ccgggcgcgg acgaccttga cgcattctgg gaccagctcg
     3061 caggcggctg caacgccatt ggacctctgc cgatgagccg ttacggcgaa gcctaccagg
     3121 agctgctgcg acatgccgat tttcccaagc gggcaggcat cctgcacggt atcgaccgct
     3181 tcgacgccgc cttcttcaat atctcgccca ccgaagccga gctga
//
LOCUS       DAOSIT010000249         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26256_length_3206_cov_8.998413, whole genome shotgun sequence.
ACCESSION   DAOSIT010000249 DAOSIT010000000
VERSION     DAOSIT010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26256_length_3206_cov_8.998413"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1465
                     /locus_tag="PLL14_06880"
     CDS             290..1465
                     /locus_tag="PLL14_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HOG03687.1"
                     /translation="MKRNRFSSRKRISADATELSRLAIGLAESGSKLEDLFWQNRLAE
                     LVDRLFQEGSEDDFTGSLDRLFDAHPMAHDDLADIIESQAESCVMTDRGHALDILLFA
                     APVLAWSRFSIPATAIPKGTLQTLKVHLCAHVFAAGSRVALADYLYSPDQLPHSFVET
                     WQLLQQLGKAALASSDLTIDASSMPETNRFLSDTRYVVGALAIPRGTPIFRWNEADKI
                     TREASLKEWIRQGGPCLEPLLTGCAYQPLLADAYHAACRSADSASRPYSLNASVAFLQ
                     STLGQPVESLRAVIGGFHDKRLEEYRVGFGPHDSEMIYHGVVWPLLGIEDETSDVAAE
                     IEAALRKSGLKEVVVLDQQFPYEFCDDCGAPLYPNIEGDTVHAEMPEQNNAPSQTLH"
     gene            1481..2812
                     /locus_tag="PLL14_06885"
     CDS             1481..2812
                     /locus_tag="PLL14_06885"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOG03688.1"
                     /translation="MSNSDLLQRSLNAVWHPCTQMQQHAAAADALPMVPIARGAGVWL
                     YDFDGRRYLDGISSWWVNLFGHCHPRINAALREQLEELEHVILAGFTHRPVIELSERL
                     SALTGGTLGHCFYASDGASATEIALKMSFHSWQNRGRTDKQEFLCLAGSYHGETVGAL
                     AVTDVALFKDAYAALIRPACTVPTPDARLAEAGETAVDVARRAAAALERHLEEHHRRI
                     AALIVEPLIQCASGMAMYDPEYLRLARALCDRYEVHLICDEIAVGCGRTGTFFAHEQA
                     GIRPDFLCLSKGISGGYLPLSIVMTTAAVYADFYDPATSRGFLHSHSYTGNALACRAA
                     LATLDIFADDQVIDANRVLAEHLGNCLRPLAAHPQVRHLRQRGMIAAFDVLSDDPQFS
                     QRFYRQALAHEILLRPIGNTVYFMPPYAISAAECEFLVTRATAALDTTLHT"
     gene            2809..>3206
                     /locus_tag="PLL14_06890"
     CDS             2809..>3206
                     /locus_tag="PLL14_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03689.1"
                     /translation="MIWDELQSALDALQRDGLKRNRRTLESPCGPVAQVAGRTLISFC
                     SNDYLGLANDPALVAAACVGARTWGVGSGASHLVSGHFAAHATLEEKLAAFTGLARAL
                     LFSTGYMANLGIVPALVGRGDAVFADRLNHA"
BASE COUNT          550 a         1161 c          965 g          530 t
ORIGIN      
        1 atcgcgagcg cctgctgagc ttcgcggagg cccgcaacac cattcgcgag tatcttgagc
       61 aacaacagca cagcctctgc cagaaggcct ggatcgccac cctgcgccgg ccgggcatgg
      121 gcgcaacccc gccgtcggcc tagaccaggc ccgcacaggt cggtctgctg cggggaatcg
      181 ccgcgaccct gccacgacag ccggcacagg caagaagatg catgcgcgcc aacgtcaggc
      241 tcgccagctt ggtacaatga cggattgacc gtcttccagg ctccagacca tgaaacgcaa
      301 ccgcttctcc tcccgcaagc gcatttcggc cgacgccacc gagctcagcc ggctggccat
      361 cggcctggcc gaatccggca gcaagcttga ggatctgttc tggcagaacc gcctcgccga
      421 actggtggac cggttgttcc aggaaggctc ggaggatgac ttcaccggct cgctggaccg
      481 cctcttcgac gcccatccga tggcccatga cgatctcgcg gacatcatcg agtcgcaagc
      541 cgagtcgtgc gtgatgaccg atcgcggcca cgcgctcgac atcctgctgt ttgcagcgcc
      601 ggtactcgcc tggtcgcgct tttcgatccc ggccacggcg atcccgaagg gcaccctgca
      661 gacgctgaaa gtccatctct gcgcgcacgt cttcgccgcc ggcagccgcg tggcgctcgc
      721 cgactatctg tacagcccgg atcaactgcc gcacagtttt gtcgagacct ggcagttgct
      781 gcaacagctt ggaaaagcgg cgctggcgag cagcgatctg accattgacg cgtcctcgat
      841 gcccgaaacc aaccgctttc tctcggatac ccgttatgtg gtcggggcgc tggccattcc
      901 gcgcggcacc cccatttttc gttggaacga ggccgacaag atcacccgtg aagcgtccct
      961 caaggagtgg atccgacagg gcggcccctg cctcgaaccc ctgctcaccg gttgcgccta
     1021 tcaaccgctg ctggccgacg cctaccatgc ggcctgccgc tcggccgaca gcgcctcgcg
     1081 gccctactcg ctgaacgcgt cggtggcgtt tctgcaaagc accctcggcc aacccgtcga
     1141 aagtctgcgc gcggtgatcg gcggcttcca cgacaagcgg ctggaggaat accgcgtcgg
     1201 tttcggcccg cacgacagcg agatgatcta tcacggggtg gtctggcccc tgctgggcat
     1261 cgaagacgaa accagcgatg tcgccgctga aatcgaagcc gcactgcgca agtccggcct
     1321 caaggaagtg gtggtgctcg accagcaatt cccctacgag ttctgtgacg actgcggcgc
     1381 accgctctac cccaacatcg aaggcgatac ggtacacgcc gagatgccgg agcagaacaa
     1441 cgccccgtcg caaacgctgc actgaaacgg gccagtaccg atgagcaaca gcgatctgct
     1501 gcagcgcagc ctcaacgctg tctggcatcc ctgcacgcag atgcagcagc atgcggcggc
     1561 ggccgacgcc ctgccgatgg tgccgatcgc caggggcgcc ggcgtctggc tgtatgactt
     1621 cgatggccga cgctacctgg acggcatcag ctcgtggtgg gtcaacctct tcggccactg
     1681 ccatccgcgg atcaatgccg cgctgcgcga gcaactcgaa gaactggagc acgtcatcct
     1741 cgccggcttc acgcatcggc cggtgatcga gctttccgag cgtttgtcgg cattgaccgg
     1801 cgggacgctg gggcactgct tctacgcctc cgacggcgcg tcggcgaccg aaatcgcgct
     1861 caagatgtcg ttccactcgt ggcagaaccg tggccgcacc gacaagcagg aattcctgtg
     1921 cctggccgga agctatcacg gagaaaccgt cggcgccctg gcggtcaccg acgtcgcgct
     1981 gttcaaggac gcctacgcgg cgctgatccg cccggcgtgc accgttccga cgccggacgc
     2041 acgcctcgcc gaagccggcg aaacggccgt cgacgtcgcc cgccgtgcgg ccgccgccct
     2101 cgaacgccat ctcgaagagc atcatcgacg catcgcggca ctgatcgtcg agcccttgat
     2161 ccagtgcgcc agcggcatgg cgatgtacga cccggaatac ctgcgtctcg cccgcgcgtt
     2221 gtgcgaccgc tacgaggtgc atctgatctg tgacgagatc gccgtcggct gcggtcgcac
     2281 cggcaccttc ttcgcccacg aacaggccgg catccgcccg gacttcctgt gcctgtccaa
     2341 gggcatctcc ggcggctacc tgccgctgtc gatcgtgatg accaccgcgg cggtgtacgc
     2401 ggatttttac gacccggcca ccagccgcgg cttcctgcac tcgcactcct acaccggcaa
     2461 cgcgctggcc tgtcgcgctg ccctggcgac gctcgacatc ttcgccgacg accaggtcat
     2521 cgacgccaac cgcgtcctcg ccgagcacct cggcaactgc ctgcggcctc tggccgcgca
     2581 cccgcaagtc cggcacctgc gccagcgcgg catgatcgcg gccttcgacg tgctcagcga
     2641 cgatccacag ttctcgcagc gcttctaccg acaggccctg gcgcacgaaa tcctgctgcg
     2701 gccgatcggc aacaccgttt atttcatgcc cccgtacgcg atcagcgccg ccgagtgcga
     2761 atttctcgtg acgcgtgcca ccgccgccct ggacaccacg ctgcacacat gatctgggac
     2821 gaactgcaaa gcgcactcga cgccctgcag cgcgacggcc tgaagcgcaa ccgccgcacc
     2881 ctcgaaagcc cttgcggacc cgtcgcgcaa gtcgcgggcc ggacgctgat cagcttctgc
     2941 agcaacgatt acctcggcct ggccaatgac ccggcccttg tcgcagccgc ctgcgtcggc
     3001 gccaggacct ggggggtcgg cagcggcgcc tcgcatctgg tcagcggcca cttcgccgcg
     3061 cacgccacgc ttgaggagaa gctcgcggcg tttaccggct tggcgcgtgc gctgctcttt
     3121 tccaccggct acatggccaa tctgggtatc gttccggcgc tggtcgggcg cggcgacgcc
     3181 gtcttcgccg accggctcaa ccacgc
//
LOCUS       DAOSIT010000250         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26421_length_3189_cov_9.615507, whole genome shotgun sequence.
ACCESSION   DAOSIT010000250 DAOSIT010000000
VERSION     DAOSIT010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26421_length_3189_cov_9.615507"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLL14_06895"
     CDS             complement(<1..212)
                     /locus_tag="PLL14_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOG03690.1"
                     /translation="MRIHALNVEEALASLKTVAGGLSTGEAARRLAEFGPNHLEEVER
                     EPLLLRCAREFTHFFAIILWIGAGLA"
     gene            complement(303..1964)
                     /locus_tag="PLL14_06900"
     CDS             complement(303..1964)
                     /locus_tag="PLL14_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03691.1"
                     /translation="MPAESKALDQRVSHFRQILMWPLQLMPLPADAPIDSHSQLLEAA
                     SPDNPWHELVDEFSGDPGQFHERHYSEFVTFLPYVQRFLYGEGGGQKAIARYGESPIR
                     IFRRHDVAKVRMTFKEGDEALSFEIVHLDLYFFYDIDVVILVVEICADDLELRQVQDT
                     LYRFGRAYPPYWDDDGSGGHCLKRCEWLAADGQSLSVSDYEKRSKYLSFVSRFRAPCI
                     SSHWEFLLRPLVLHHSDAKGLVRYRQIEYHRMPLLAYLAMDDPRTLSRGDFARLVLVT
                     GPGKQGSLPYSDQHLEGFESRYCIDRFWHPQADDPGTRLLSCGHAFVMVGSAHDDYFT
                     GLETGLLGQFRHQFFLLALIPHFHKAALLMLSDRLVTALNRLEIGNAESVKQFKRAIR
                     QILEVFLRFSHRYWFHEISDQAQARALFSMTREHLGTENLYTELREEIQDMSQYLDSD
                     SLRRQANTVVRLTVVTTAGLVATITTGFLGINLIDAAQDGLVDRILFFALVAIPSVLL
                     TGFAVVKSKPLSDFLEALSDERLSHRDKLAALLPVWRKKRPPDAG"
     gene            complement(2060..2977)
                     /locus_tag="PLL14_06905"
     CDS             complement(2060..2977)
                     /locus_tag="PLL14_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HOG03692.1"
                     /translation="MRPDQERLDVPLAAALCEAIGGGTGSPANIDSLTGIAGGSISRA
                     LLVESAGERYFVKLNDAELADMFSAEADGLRALAACPALRVPQVLGHGVSGRQAYLLL
                     EYLPLQALRTDQHGAKAGHALAALHRLHGPQYGWHRDNFIGSTVQLNAEHRTWPFFFA
                     HRRLLPQLELARQHAQYNRLIADGERLAGKLAALFVDHHPQPSLLHGDLWHGNAATDE
                     TGTLTLFDPAVHFGDREADLAMSELFGGFPDSFYAGYREAWPLNDGYEQRKTLYNLYH
                     VLNHLNLFGSGYLRQAERMVATLLAEIGC"
BASE COUNT          634 a          943 c         1018 g          594 t
ORIGIN      
        1 aaggccagac cggcgccgat ccacaggatg atggcaaaga agtgggtgaa ctcgcgtgcg
       61 caacgcagca gcaggggttc ccgttccacc tcttcgagat ggttcgggcc gaactccgcc
      121 agacgacgcg ccgcttcgcc ggtgctgagg ccgccagcca ccgttttcag gctggccagt
      181 gcctcttcga cgttgagagc gtgaatgcgc aaggggcgaa actcctgcta atggcgcgaa
      241 gccagacagc aatttacacc cttgtccgaa acaaagcatc tgccgagatg gtctggatta
      301 cttcagccgg cgtccggcgg gcgcttcttt cgccatacgg ggagcagcgc ggcgagcttg
      361 tcgcgatgcg acaggcgttc gtcggacagc gcctcaagaa aatccgataa cggtttcgac
      421 ttcacgaccg cgaagccagt gagcagtacc gacggaatgg ccaccagggc aaagaagagg
      481 attctgtcga ccaacccgtc ctgggcggcg tcgatcagat tgattcccag gaagccggtg
      541 gtaatcgtcg ccaccagccc ggcagtggtc accaccgtca accgcaccac cgtgttggcc
      601 tgccggcgca ggctgtcgct gtccaggtac tgactcatgt cctggatttc ctcacgcagc
      661 tcggtgtaga gattttcggt gcccaggtgt tcacgggtca tgctgaacag cgctctggcc
      721 tgtgcctgat ccgagatctc atggaaccag tagcgatggc taaagcgcag gaagacctcg
      781 aggatctgcc ggatggcgcg tttgaattgc ttgaccgatt cggcgttgcc aatctccaga
      841 cgattgagcg ccgtgaccag acgatccgag agcatcagca gcgccgcctt gtggaaatgc
      901 ggaatcagtg ccagcaggaa gaactggtgg cgaaactgcc cgagcagacc ggtttccagt
      961 ccggtgaagt aatcgtcatg ggcgctgccg accatcacga aggcgtggcc gcaggacagc
     1021 aggcgcgttc ccggatcgtc ggcctgtgga tgccagaagc ggtcgatgca gtagcggctt
     1081 tcaaaacctt cgagatgttg gtcggaatag ggcagggagc cttgctttcc aggtccggtg
     1141 accaggacca ggcgggcaaa gtcgccgcga ctcaaggtgc gaggatcgtc catcgccaga
     1201 taggccagca gcggcatacg gtgatactcg atctgccggt agcggaccag tccctttgca
     1261 tccgagtggt gcagtaccag agggcgcagc aaaaattccc agtgcgacga aatgcacggt
     1321 gcacgaaagc ggctgacgaa agacaggtac ttgctgcgct tctcgtagtc ggaaacggac
     1381 agcgactgac cgtccgccgc caaccactcg caacgtttga ggcaatggcc gccgctgcca
     1441 tcgtcgtccc agtacggcgg ataggcgcgt ccgaagcgat acagcgtgtc ctgtacctgt
     1501 cgcagttcga ggtcgtcggc gcaaatttcg acgaccagga tgacgacgtc gatgtcgtag
     1561 aagaaataca ggtcgaggtg cacgatctcg aagctgagcg cctcgtcgcc ctccttgaag
     1621 gtcatccgta ccttcgccac gtcatgacgc cggaaaatgc ggatcggtga ctcgccgtag
     1681 cgcgcgatgg ccttctggcc tccgccctca ccgtaaagaa agcgctggac atacggcagg
     1741 aaggtgacga actcgctgta gtggcgctcg tggaattgac cagggtcgcc ggagaactcg
     1801 tcgacgagtt catgccacgg attgtcgggt gaggccgctt cgagcaactg gctatggctg
     1861 tcgatgggcg catcggcggg caacggcatc aactgcaacg gccacatgag gatctgccgg
     1921 aaatgactga cccgctggtc tagtgccttg ctctctgctg gcatgttcac ctccaagaag
     1981 aatccaagaa tagatccgtg cgacggttgc tcggcggctg gtcatcactg aaggctgctg
     2041 cgctcacctt gtccggtaat cagcagccaa tctcggcaag caacgtagcg accatgcgct
     2101 cggcctggcg aagatagccg ctgccgaaca ggttcaagtg attgagcacg tgatagaggt
     2161 tgtacagcgt cttgcgctgc tcgtaaccgt cgttcaatgg ccaggcctcg cggtagcccg
     2221 cgtagaaact gtccggaaaa cctccgaaca gctcgctcat cgccagatcg gcttcgcggt
     2281 cgccgaaatg gacggccgga tcgaacaggg tcagggtgcc cgtctcgtcg gtcgcggcgt
     2341 taccgtgcca caggtcgcca tgcagcaggc tgggttgggg gtgatggtcg acgaacaatg
     2401 ccgcaagttt gccggccagg cgttcaccgt cggcgatcag ccggttgtac tgggcgtgtt
     2461 gcctggccag ttcgagctgt ggcaacagac gccggtgggc aaagaagaag ggccaggtgc
     2521 gatgctcggc attaagctgt actgtgctgc cgatgaagtt gtcacgatgc cagccgtact
     2581 gcggaccatg caggcggtgc aatgcggcca gtgcgtgccc ggcctttgcc ccgtgctggt
     2641 ctgtacgcaa ggcttgcagc ggcaggtatt cgagcagcag ataagcctgc cggccgctca
     2701 cgccgtgtcc taacacctgt ggcacgcgca gtgcgggaca agcggccagc gcgcgcaggc
     2761 catcggcctc ggcagagaac atgtcggcca gttcggcgtc gttcagtttg acgaagtagc
     2821 gttcgccggc gctttccacc agtagcgcgc gcgaaatcga accgccggcg attccggtca
     2881 gcgagtcgat gtttgccggt gaacccgtac ccccgccgat ggcctcgcac agcgccgcag
     2941 caagcggcac atcgagccgt tcctggtctg gtctcatgtc cctccttgcg gctcgagtcg
     3001 aaagccgtcc atcatcgcga ccctggagct atcgctgggg gccgggttta ctcgtgccgg
     3061 ggaatgtgtt gtgataaggc gatgcgtgca ggctgtcggt gaccacgcac caggcaggca
     3121 tggcacggcc gcggttcgcg cgacgcgttc tcgaaacggc ccgtggtttc tctctatcca
     3181 gatgacttc
//
LOCUS       DAOSIT010000251         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26646_length_3168_cov_8.026662, whole genome shotgun sequence.
ACCESSION   DAOSIT010000251 DAOSIT010000000
VERSION     DAOSIT010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26646_length_3168_cov_8.026662"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLL14_06910"
     CDS             <1..462
                     /locus_tag="PLL14_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03693.1"
                     /translation="LSAFTYGSAKAITSSQHESAKQGLEKAKGEASAAQGNADAPGAA
                     AGLQDLAAHAAGLQSQAQQQVDLKTKGTPEMKNLFCDDLGNLDFGDSQMFFIVLLTVA
                     IFILTSFHFLEWVAYSPQIILPDLDTTLLSTFGLGQGAYLVKKAASPLGKG"
     gene            497..1228
                     /locus_tag="PLL14_06915"
     CDS             497..1228
                     /locus_tag="PLL14_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOG03694.1"
                     /translation="MIDTILLTAARICTFAQQRALTNASGFFFERAGRLFLVTSRHVM
                     LDKPSRHFPDRLEIELHIDPDNMAGSTGFSIPLYREGKSLWRQGLDTAGDIDVALIEI
                     DRYALPTSTVYRAFTPEHLPGPFDQVEVGSSLLVVGFPLGFHDTLHHMPVVRQAVIAS
                     SFGLRFKGEGYFLTDARTHRGTSGAPVVMRVPGHDPRRRDLPWMLLGIHSARLDVGTR
                     DRKLDEALGLNSAWYADILMTLSAA"
     gene            1228..>1739
                     /locus_tag="PLL14_06920"
     CDS             1228..>1739
                     /locus_tag="PLL14_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG03695.1"
                     /translation="MSSFPLAPRYRQTLQMLSFAWRMLRRDARGGELRLLAAALVVAV
                     AALTAVGFFADRVRQALDRESHQLLGADLLLTADHPWPEKLVGDARALGLQVVETRTF
                     PSMVTRGEGDELRAQLAEIKAVGATYPLRGALRIAPALNVADAPAVGVPGPGTIWVDE
                     RLASSLGARVG"
     assembly_gap    1740..1839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1840..>3168
                     /locus_tag="PLL14_06925"
     CDS             <1840..>3168
                     /locus_tag="PLL14_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG03696.1"
                     /translation="LVRGQRLEDPQNGRPEIRTALDRAQKFLGLSALLTVVLAAVAVA
                     LASRRYVQRHLDPCAVMRCLGATQALLLRLYLAQFVVLGVLAAAGGCVLGYLAHFALH
                     AWLAQLLATPLPPPGLLPAAQGVLVGLLLLFACAVPPLLQLKRVSTLRVLRRELGSPR
                     VGLLGGYLSGFAALAMLMFWVAGEIALGAYVVTGFSVALFVFALLARLVVRLAAAARG
                     GVSAGRSAGIGWRYGLASLERRASTSVVQIVALALGFMALLLLTVTRGDLLDAWQRAV
                     PPDAPNRFVVNIQPEQVEGVRALLVAQGLSPELAPMIRGRLTTINGVEVSAASYTDDR
                     AQRLVDREFNLSVRADLPEGNRLSAGRWFSPADLGGKGASAVASVEEGLANTLGLSVG
                     DRLEFVVAGDRIGMTVVGLRKVNWDTMRANFFVLTPPVVLDGYPASWITS"
BASE COUNT          467 a          947 c          995 g          659 t
ORIGIN      
        1 ctgagcgcgt ttacctatgg ctcggcgaaa gcgataacct ccagccagca cgaaagtgcc
       61 aaacaagggc ttgaaaaggc aaaaggcgag gccagtgccg cccaagggaa cgccgatgct
      121 cccggtgcag cggcaggttt gcaggacctc gcggcgcatg cggcaggtct ccagagtcag
      181 gcccagcagc aggtagactt gaagaccaaa ggtacaccgg aaatgaagaa tctgttttgc
      241 gatgacctgg gtaatctcga tttcggcgac agtcagatgt ttttcatcgt cttgctgact
      301 gttgcgatct ttatcctcac ctcgtttcat tttcttgagt gggtagccta ttcgccgcaa
      361 atcatcctgc ccgacctcga tactacgctg ctgtctacgt tcggactggg acagggcgcg
      421 tatctggtga agaaggccgc cagcccattg ggcaagggct agggagtcat caccttgcca
      481 ccgtaccgtt gacctgatga tcgataccat cctgctgacc gctgcgcgca tctgtacgtt
      541 tgcccagcaa cgcgcgctga ccaatgccag cggttttttt ttcgagcgtg ccggccggct
      601 gtttctggtc accagccggc acgtgatgct cgacaagccg agccgacatt ttcctgatcg
      661 tctcgagatc gagttgcaca tcgaccccga caacatggcc ggctcgacgg gcttttcgat
      721 tccgctgtac cgcgagggaa agagcctctg gcgccagggc ctggacactg ccggagacat
      781 cgacgtggcg ttaatcgaga tcgaccgcta cgcgctcccg acgagtacgg tttatcgcgc
      841 ctttaccccc gagcatctgc ccggcccctt cgaccaggtc gaagtcggca gttcgctgct
      901 ggttgtcggt ttcccgcttg gtttccacga tacgctgcat cacatgccgg tggttcgcca
      961 ggccgtgatc gcttcctcat tcggtctccg attcaagggt gaaggctact tccttaccga
     1021 tgcgcgtacc caccggggga ccagtggcgc gccggtggtg atgcgtgttc ccggtcacga
     1081 ccccaggcga agggacttgc cgtggatgct gctcggaatc cattccgccc ggcttgatgt
     1141 cgggacccgg gatcgcaagc tcgatgaagc ccttggcctg aacagcgcct ggtacgcgga
     1201 catcctgatg acgctaagcg cagcttgatg agttcgttcc cgctcgcgcc gcgctaccgg
     1261 cagacgcttc aaatgctgtc ctttgcctgg cgaatgttgc gccgtgacgc acgcggcggt
     1321 gagttgcgct tgctggcggc ggcgctggtg gtcgccgtcg ccgccctgac ggcggtcggc
     1381 ttcttcgccg accgcgtgcg tcaggcactc gaccgtgagt cccatcagtt gctcggtgcc
     1441 gatctgttgc tgaccgccga ccatccctgg cctgaaaagc tggtcgggga tgcgcgggcc
     1501 ctcggtctgc aggtcgtcga gacacgtacc tttccaagta tggtgacacg cggcgagggt
     1561 gacgagttgc gggcacaact ggcggagatc aaggccgttg gcgccactta tccgctgcgt
     1621 ggcgccttgc gcattgcgcc ggcgctgaac gtcgccgatg ctccggcggt tggcgtgccg
     1681 ggtccgggca cgatctgggt cgacgagcgt ctggcctcgt ctctgggtgc gcgcgttggn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttggtgcgcg ggcaacggct
     1861 cgaagaccct cagaacggtc gcccggagat ccgcaccgcg ctcgatcggg cgcagaaatt
     1921 cctggggctc tcggcactgc tgacggtcgt tctggcggcg gtggcggtcg ccctcgcatc
     1981 gcgacgctat gtgcagcgcc atctcgatcc ctgcgccgtc atgcgctgtc tcggtgctac
     2041 ccaagctctg ctgttgcgcc tgtatctggc acagtttgtg gttctcggcg tgctcgccgc
     2101 agccggtggc tgtgtactcg gctacctcgc tcatttcgcg ctgcacgcct ggctggcgca
     2161 gcttctggcg acgccattgc cacctcccgg actgttgcct gcggcacagg gcgtactggt
     2221 cggtttgctg ctgctgttcg cctgcgccgt gccaccgctg ctgcagctga aaagggtctc
     2281 gacgctacgg gttctgcgtc gcgaattggg aagtccacgc gttggtctgc tcggtggtta
     2341 tttgtcgggt ttcgcagcac tggcgatgct gatgttctgg gttgccggcg agatcgcgct
     2401 cggtgcctat gtggtcacgg gcttcagcgt ggccctgttc gtctttgcgc tgctggcccg
     2461 cctcgtcgtc aggctggccg ccgcggcgcg cggcggtgtg agtgccggaa ggtcggctgg
     2521 gatcggctgg cgctacggtc tggctagtct ggaaaggcgg gcaagtacca gcgtcgtcca
     2581 gatcgtcgcg ctggcgctgg gcttcatggc gctgttgctg ctgaccgtga cacgcggcga
     2641 tctcctcgac gcgtggcagc gagcggttcc tcccgatgcg cccaatcgct ttgtcgtcaa
     2701 tatccagccc gaacaagtcg aaggggtacg cgcgttactg gtggcgcagg gcttgtcacc
     2761 cgagttggcg ccgatgattc gcggccgcct gacgaccatc aacggcgtcg aagtcagtgc
     2821 cgcaagctac accgacgatc gggcacagcg cctcgtcgat cgcgagttca acctgtcggt
     2881 gcgcgcggat ctccccgagg gcaacagact cagtgccggg cgctggtttt cacctgccga
     2941 tcttggcggc aagggagcct ccgcggttgc ttctgtggaa gagggcctgg ccaacaccct
     3001 gggtctgagc gtcggcgacc gcctcgagtt cgtcgtcgcc ggtgacagaa tcggcatgac
     3061 ggtggtcggt ttgcgcaagg tcaattggga tacgatgcgt gcaaatttct ttgtcctgac
     3121 gccgccggtg gtcctcgatg ggtatccggc aagctggatc accagctt
//
LOCUS       DAOSIT010000252         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26748_length_3159_cov_7.872423, whole genome shotgun sequence.
ACCESSION   DAOSIT010000252 DAOSIT010000000
VERSION     DAOSIT010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26748_length_3159_cov_7.872423"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PLL14_06930"
     CDS             <1..90
                     /locus_tag="PLL14_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOG03697.1"
                     /translation="LRDLIRRGDVSANVDMKPGDVLIIPQSWF"
     gene            187..1746
                     /locus_tag="PLL14_06935"
     CDS             187..1746
                     /locus_tag="PLL14_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOG03698.1"
                     /translation="MDELIRQALTVLRGMWKRRWLGVVVAWVVGVASVAAVLAIPDKY
                     EASARIFVDTQSILRPLMSGLVTQPNVDQQVMLLSRTLINRPNVEKLIRMADLDLNIQ
                     SKVDREALVETLMSSLKINNTSRDNIYTITYRDTQPEHAKRVVQSLVSIFVESNLGDS
                     RKDTEAARKFLDKEIAGYEKKLEEAEARLKEFKLRNLANQNSEGKDHFAQMSATGALL
                     EQSRLELREAQNSRDALRRQIQGEEPTVLPDVSQEPIAGVAVPEIDSRIDALKRNLDA
                     MLQRFTDKHPDVVSTRRLIKDLEEQKQQEIVARRKTAVTNPSVSVNANPVYQQLKVTL
                     SEMEARVAALQTRVGEYQARYDRLKSAISLVPQIEAEFTQLNRDYEINRKNYEQLVQR
                     RESASLGSEMDNTAGVDFRLIDPPRASPKPVAPNRNLFLPLTFLLALVAGVAATFAAS
                     QLRPVFFDSRSLREACGLPLLGTVSMLIDEESQRRDRKDLLRFAAACGSLVAAYGAGL
                     LALFLISVRTA"
     assembly_gap    1755..1854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1855..2556
                     /locus_tag="PLL14_06940"
     CDS             <1855..2556
                     /locus_tag="PLL14_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HOG03699.1"
                     /translation="APRSQLADQYRVIKRPLIANAMGKGASPIKRGNLIMVTSAVSGE
                     GKSFTAINLAMSIAMELDNTVMLVDADVPKPSVLKMLGLPPSRGLLDVLTDESVALSQ
                     VMMRTSVDKLSILPSGTQHPRATELLASEAMVKLLDEMANRYPDRIIIFDSPPLLMTT
                     EARVLASHMGQVVVVVKAESTVRSEVKHALAAIDSCPVKLVLLNQARTSTQDGYGYGY
                     GYGYGYGYGKVAEDA"
     gene            2549..>3159
                     /locus_tag="PLL14_06945"
     CDS             2549..>3159
                     /locus_tag="PLL14_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HOG03700.1"
                     /translation="MLEGTTGQALFAGPRSRRYPVDAATVRDGRRYPAPVSWLGFALI
                     CGAIPAAHAENWRITPTLAVNETLTNNLYLTSSNRTSDLVTGITPGISIEGKGARASL
                     RLSYAFTEQLYARESSSNGHQNALTAIGTLEAIDDWLFIDATGSISQQYLSAFGAISP
                     STANVNRNQTETSSYSISPYIKGRFLTSTEYFLRYKGTTTSSQS"
BASE COUNT          613 a          929 c          940 g          577 t
ORIGIN      
        1 ttgcgtgatc tcatccggcg gggtgacgtg tcggcgaatg tggacatgaa acccggcgac
       61 gtgctgatca ttccgcagag ctggttctaa gtcggccggt tcgccttgca atgccctacg
      121 tgcttttcct ggcgtcgctg aacgtcggga atacccctga aacgatactg ataaagtctg
      181 aatgcgatgg atgaactgat tcgacaggcg ttaacggtcc tgcgcgggat gtggaagcgt
      241 cgctggctgg gggtcgtcgt cgcctgggtc gtgggcgtgg cgagcgtggc ggcggtcctg
      301 gccattcccg acaagtacga agcgtcggcg cgcatctttg tcgacaccca gtcgattctc
      361 cggccgttga tgtccggtct ggtgacacag ccgaacgtcg atcagcaggt catgctgctc
      421 agccgcacct tgatcaaccg gccgaacgtt gagaaactga tccggatggc cgacctggat
      481 ctcaacatcc agtcgaaagt ggatcgggaa gcgctggtgg aaacactgat gtcgtcgctg
      541 aagatcaaca atacttcgcg cgacaatatc tacaccatca cctaccgcga tacgcagccc
      601 gagcacgcca agcgcgtggt gcaatcgctg gtctcgatct ttgtcgaatc gaatctcggt
      661 gactcccgca aggataccga agcggcacgc aagtttctcg acaaggagat cgccggctac
      721 gagaagaaac tcgaggaggc cgaggcgcga ctgaaggaat tcaagctgcg caatctggcc
      781 aaccagaatt ccgagggcaa ggaccacttc gcgcagatga gcgccaccgg tgcattgcta
      841 gagcagagcc ggctcgaact acgcgaggcc cagaattcgc gtgacgcgct caggcgccag
      901 atccagggcg aggagccgac cgtgctgccg gacgtaagcc aggagccgat tgccggagtg
      961 gcggtgccgg agatcgacag tcggatagac gccctcaagc gcaatctcga cgcgatgttg
     1021 cagcgtttca ccgacaagca ccccgacgtc gtgagcacgc ggcgcctgat caaggatctc
     1081 gaagagcaga agcagcagga gatcgtcgcc cgccgcaaga cggcggttac caatcccagc
     1141 gtttcggtca acgccaatcc cgtgtaccag caacttaagg ttaccctctc cgaaatggag
     1201 gcgcgggtcg cggcgctgca gacacgtgtt ggcgagtatc aggcgcgcta tgaccggctg
     1261 aaatcggcga tcagtctggt gccccagatc gaggcggagt ttacgcagct gaaccgcgac
     1321 tacgagatca accggaaaaa ctacgaacaa ctggtgcagc ggcgtgaatc ggccagcctc
     1381 gggagcgaga tggacaacac cgctggcgtc gattttcgcc tgatcgatcc accgcgcgct
     1441 tcacccaagc cggtggcccc gaaccgcaac ctgttcctgc cgctgacatt ccttctggcc
     1501 ctggtagccg gggtggctgc cacttttgcg gccagtcagc tccgaccggt gtttttcgat
     1561 tcgcgttcgc tgcgggaagc ctgcggacta ccgctgctcg ggaccgtctc catgctcatc
     1621 gacgaggaaa gccagcgtcg cgacaggaag gatttgctgc gctttgctgc tgcctgtgga
     1681 tcactggtcg cggcctatgg cgcgggactt ctcgccttgt ttcttatttc cgtccgtact
     1741 gcctgaggaa caccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgccg
     1861 cgatcgcagc ttgccgatca gtatcgggtc atcaagcggc ccctgatcgc caacgccatg
     1921 ggcaagggag cttcgccgat caagcgcggc aacctgatca tggtgaccag cgctgtttcc
     1981 ggtgagggca agagcttcac cgcgatcaac ctggcgatga gcatcgccat ggaactggac
     2041 aacacggtga tgctggtcga tgccgacgtg ccgaagccat cggtgctcaa gatgctcggc
     2101 ctgccgccgt cgcgcggcct cctcgatgtg ctcaccgacg agtcggtggc gctgtcgcag
     2161 gtcatgatga ggaccagcgt cgacaagttg agcatccttc cgagtggtac gcagcatccg
     2221 cgcgcgaccg aactgctggc cagtgaggcc atggtcaagt tgctcgacga gatggccaac
     2281 cgttatcccg accggatcat catcttcgat tcgccgccgc tgctgatgac caccgaggcg
     2341 agggtgctgg ccagccacat ggggcaggtc gtcgtcgtcg tgaaggccga aagtaccgtg
     2401 cgcagtgagg tcaagcacgc tctcgccgcg atcgactcgt gcccggtgaa actggtgctg
     2461 ctcaaccagg cgcgcaccag cacgcaggat ggttacggct atggttatgg ttacggctat
     2521 ggttacggct atggcaaggt tgccgaagat gcttgaaggg acgaccggac aagccctttt
     2581 cgccgggccg cggagcaggc gttaccctgt tgatgcagcc accgttcggg acggccggcg
     2641 ctacccggcg cccgtatcat ggctgggttt tgcgctgatt tgcggtgcca ttccggcggc
     2701 gcatgccgag aactggcgga tcacgccgac ccttgccgtc aacgagacgc tgaccaacaa
     2761 tctttatctc acttccagca accggaccag cgatctggtc accggcatca ccccggggat
     2821 ctcgatcgaa ggcaaggggg cgcgtgccag cctgcgcctg agttacgcct ttaccgaaca
     2881 gctctatgcc cgggagtcct cgtcgaacgg gcatcagaat gcgctgacgg cgatcgggac
     2941 gctggaagcc atcgatgact ggttgttcat cgatgcgacg ggcagcatct cgcaacagta
     3001 tctttccgcc tttggcgcga tttcgccgag caccgccaac gtcaaccgta accagacgga
     3061 aacgtcgagc tactcgattt ctccctatat caagggcagg ttcctgacct ccaccgagta
     3121 cttcctgcgc tacaagggaa cgacgacctc ctcgcaatc
//
LOCUS       DAOSIT010000253         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26881_length_3146_cov_7.123585, whole genome shotgun sequence.
ACCESSION   DAOSIT010000253 DAOSIT010000000
VERSION     DAOSIT010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26881_length_3146_cov_7.123585"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..421)
                     /locus_tag="PLL14_06950"
     CDS             complement(26..421)
                     /locus_tag="PLL14_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03701.1"
                     /translation="MISNEDARSFSSARTMALPSGWFGKLMAIMASATLVVVGLMFSV
                     LALAVLLVGGSLLFVYLKWKTRHLRRVLDAQMQLARAPEQQSSGRVIEGEIIGDAEYD
                     ANSHAPARNPLLNDVPASREQQPESATDR"
     gene            1246..1857
                     /locus_tag="PLL14_06955"
     CDS             1246..1857
                     /locus_tag="PLL14_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HOG03702.1"
                     /translation="MNELNRHLADTYGGGDGSPQVALGNMAEGIASAPGSSGVPRDRL
                     ILSMVNIKEEKALKNLPNHVRNDTALTVTYQNPPVFLNCQILVVASHGNYTNALLMLS
                     RAIRFFQFKNVFSQDNVAPASMTTNAPGNALDQLESFKLVLDLYSPNMEEINHLWGTL
                     GGKQYPFALYVLRLLELQFKATQREGVLVTEVVTDFYQKTVAD"
     gene            1970..2869
                     /locus_tag="PLL14_06960"
     CDS             1970..2869
                     /locus_tag="PLL14_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03703.1"
                     /translation="MKARLLQAYDVRRLLGIRPTGSTRVALAANQCLFEETALGFVVL
                     LEGTASLPADSIFEFMIVVRDSRFFDYSALTLRSQRIHEAYDRSEGVICRYKENVPVV
                     SNLSGATRGAGAGKLLFLSRRYRPLAPNEQVEALGLSGTALGQLTSDGPNARIQQLAA
                     DANDLPVYLHQGDVPAIIPPAGVVGVPARGVRLSDDVADDVFALISLAAVRPGDSAFS
                     FVDATGRPKSSPPVYQLRIRNRSTFWAYVNKTTGVVDSIESDALPLTHFGNAGQKQKP
                     SERLVKVEKTGAVISRLVSEVHV"
     gene            2908..>3146
                     /locus_tag="PLL14_06965"
     CDS             2908..>3146
                     /locus_tag="PLL14_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HOG03704.1"
                     /translation="MATAYKTPGVYIEEIPKFPPSIAPVATAIPAFIGYTRKAENRTA
                     ADLHLKPTRISSMIEYEKYFGGAQSEEAISVTVTVT"
BASE COUNT          638 a          891 c          958 g          659 t
ORIGIN      
        1 caaagggtgg aaggaactgc ggcgctcaac ggtcagttgc ggattcaggc tgctgttcac
       61 ggcttgccgg cacgtcgttc agtagcggat ttcgtgccgg ggcatgagaa ttggcgtcgt
      121 attcagcatc gccgatgatt tcaccctcga tcacacgacc gctggactgc tgttcaggcg
      181 ctctggccag ctgcatttgc gcgtccagga cgcgacgcag gtgacgtgtt ttccacttca
      241 ggtagacaaa cagcagcgat ccgccgacca acagcactgc gagagccagc accgagaaca
      301 tgaggccgac gacgaccaac gtcgcgctgg ccatgatcgc cattaactta ccgaaccaac
      361 cggaggggag cgccatcgtt cgtgcgcttg aaaaactgcg tgcgtcctcg ttgctgatca
      421 tcgaagtcct tgttttcaga ttcggcgaag agtggcgtcg ccgcctattg taaggtttcg
      481 gaagttctcc ggccatccct ggacagcact cgatgtcgcg atcgctggcg ggaggtcgct
      541 accgaccaaa agaacggccc gcgcaggggg ggctgggcgg gccggcgttg cagtcgccgc
      601 gacactccaa ggggaggggg agggggagaa agcatcccga cgacgagacg aatcctacag
      661 gcgaaagcag caggcatctg tggcgcgtat gttccattgt gtgacagcgt gtaagggctg
      721 ggcttccggt gggcttcaag ccgctaacgt cgctgttcga gtcgtgaagg gcttcccgtc
      781 tcctgcgggt cgagcggccg cttttccttg gcggctggcc gaacagttgc tcgacggcat
      841 gcacaggtcg gagcgccgcg ccggcagggt accgctgccg ctgcaattga caagcacggc
      901 tgagtatggt ttgctttgat ataaggtcgt ttcagccgga ttcgcgtacc actgtgccga
      961 ggagggcatc gatgccgcac ccctgttcca gctccaggtg ccagccgaca attgcggcga
     1021 cggatggtgt gccttggctg cagatttttg ccctcaggcc cctgccggca tggagtggcg
     1081 gccccctcgc cggaccttcc tcgacgcgtc gttgtgtctt gagcgcgggg ttccctggat
     1141 ggaagccggt agcgcctcac gcactcgcct ggcggtctgc caccgcgccg gccgctgctg
     1201 tgagcggttg ctgatgacat gatctcgcat gcattgacca tcgtgatgaa cgagttgaac
     1261 aggcatctcg ccgataccta cggcggtggc gatgggtctc cgcaggtggc tttgggcaac
     1321 atggcggaag gcattgccag cgctccaggc agcagtggcg taccccgcga caggctcatt
     1381 ctgtcgatgg tgaatatcaa agaggaaaag gcgctcaaga acctgcccaa tcatgtccgt
     1441 aacgatacgg ctttgacggt cacctatcaa aatccacctg tatttctgaa ttgccagatt
     1501 cttgtcgttg ccagccatgg caactatacc aatgccttgt tgatgttgtc gcgggcgatc
     1561 cgttttttcc agttcaagaa cgtgttcagc caggataatg tcgctccggc ttcaatgacc
     1621 accaatgcac cgggcaatgc cctcgatcaa ctcgaatcgt tcaagctcgt cctcgatctg
     1681 tattccccga acatggagga gatcaaccat ttgtggggta ccctgggcgg caagcagtat
     1741 ccctttgccc tgtatgttct gcgtcttctc gaactgcaat tcaaggccac acagagggaa
     1801 ggcgtgctgg ttaccgaggt ggttaccgac ttttatcaga aaacggtggc ggattaatat
     1861 ggcggagcgg atggcaggta gatacaggca attgttcgaa gtccggatcc ttcaccatta
     1921 ttggctggat cggggagcga gcgtctttga ccggatcacg gatcagacga tgaaagccag
     1981 actgctgcaa gcctacgatg tacgtcgttt gcttggcata cgacctacag gaagcacccg
     2041 cgttgcgctc gcggctaacc agtgcctgtt tgaagaaacg gcactgggtt tcgtggtgct
     2101 cctggaaggt accgcgagcc tgccggcgga cagcatcttc gagttcatga tcgttgtcag
     2161 ggacagtcgt ttttttgact actcggcgtt gaccttgcgt tcgcaaagga tccacgaggc
     2221 atacgaccgg agcgaaggtg tcatctgtcg ctacaaggaa aacgtgcccg tcgtgtcgaa
     2281 tctgagtggg gcgacaagag gggcaggggc ggggaaattg ctgttcctgt cgcgaagata
     2341 tcgccctctg gcacccaacg agcaggttga agccctgggt ttgtcgggta cggcgttggg
     2401 gcaactcacc agtgacggtc cgaacgcacg catccagcaa cttgccgccg acgcaaacga
     2461 cctcccggtg tacctccatc aaggtgacgt cccggcgatc atcccgccgg cgggagtggt
     2521 cggtgtgcca gcgcggggcg tcaggctgtc ggacgatgtg gcggatgacg tatttgcgct
     2581 gatcagtctg gccgccgtgc gtccgggtga cagtgcgttc agttttgtcg atgcgactgg
     2641 ccggccgaag agcagtcctc ccgtctacca gcttcgcatc aggaaccgtt cgacgttctg
     2701 ggcctacgtg aacaagacga ccggagtcgt agactcgatc gaatcagatg ctctacccct
     2761 gacccatttt ggcaacgcag gccaaaagca aaagccttcc gaacggctgg tgaaagtgga
     2821 gaagaccggc gccgtcatca gccgcctcgt ttcggaagtt catgtctaac tgcaagctct
     2881 tactctaacc acatacgggt gatattcatg gctacagcgt acaagactcc aggcgtttac
     2941 atcgaggaaa ttccaaaatt cccgccgtcc attgccccgg ttgcgaccgc gattccagcg
     3001 ttcatcggct atacccggaa ggccgagaac cgcacggcag cagatctgca cctgaagccg
     3061 acgcgcatct cgtcgatgat cgagtacgaa aaatactttg gcggcgcaca atccgaggag
     3121 gccatcagcg tgacggtcac cgtaac
//
LOCUS       DAOSIT010000254         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_26963_length_3138_cov_8.228349, whole genome shotgun sequence.
ACCESSION   DAOSIT010000254 DAOSIT010000000
VERSION     DAOSIT010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26963_length_3138_cov_8.228349"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLL14_06970"
     CDS             <1..1032
                     /locus_tag="PLL14_06970"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HOG03705.1"
                     /translation="APEHGGPEAASEEPDDDDASDVPEGDAFWRTILDPLTDVALASI
                     RLGGHLWGQFLGLRNDPAGIRDYARVAAEIAQEVGKLALLPNDSPTRFKGKPGTVKRV
                     AWSEPIALSDIKAVGKVLGCSVNDTLLAAVAGALRGYLVARGDPVVSTAAIRAMVPVN
                     LRAASDVDELGNRFGLVALELPIGIDNPLARLYATRARMAALKSSYQAMLTFALLGAA
                     GTAPKFVQDQILKLLTSKTTAVMTNVPGAQQPRYLAGNRIDQQMVWVPQAGDIGMGVS
                     ILSYNGRVQFGLITDKNRVDDPEQIVGRFADEFEKLLWLLLLEPWDRLGDPTAVEEDL
                     SRLIKTAAE"
     gene            complement(1055..1828)
                     /locus_tag="PLL14_06975"
     CDS             complement(1055..1828)
                     /locus_tag="PLL14_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03706.1"
                     /translation="MTWKTFPYPDQDYNYAGAALQENWGRLHRGDCEPFPDDPKVQDA
                     WRSYHAGDFGKAVEAGLACGLAGYNAANKAATIYASYLETDDDRKRHLLMEVAQRCEE
                     QQQKSPKVVNAWYLHAFALGRYSQSISVVKALAQGLGGKIKHSLTQALEIEPRHADAH
                     IALGAYHAEVIDKVGALVGGITYGVKKDAGKALLEAALKLNPDSAITRIEYANALVMM
                     FGKAGMKDAERLYQEAAECQPMDAMERLDVEAAKVELAD"
     gene            complement(1847..2494)
                     /locus_tag="PLL14_06980"
     CDS             complement(1847..2494)
                     /locus_tag="PLL14_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG03707.1"
                     /translation="MEKKPKRRTRERILETSLRLFNDFGEPNVTTTVIADEMDISPGN
                     LYYHFHNKDEILESIFADFEREIEDILAVPTRRSADVEDIWLFLHLLFEQIWKYRFFY
                     RDLNDLLTRNRVLEIHFKQILAHKVRTATTLCESLVFAGAMRAGPEELRALATNMTVI
                     ATYWLSFEYACDPRGKVESGRIGRGVYQVMAMISPYLFGESKGLLEKLSHEYLRA"
     gene            complement(2594..>3138)
                     /locus_tag="PLL14_06985"
     CDS             complement(2594..>3138)
                     /locus_tag="PLL14_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOG03708.1"
                     /translation="HASVALHEGAELVICINPLVPFDARLAAKRPAHENPSPAPEHLV
                     DGGLPVVLSQTFRAIIHSRMRTGMDRYRLEFEGKDVVLFEPTRDDADMFFTNVFSYRG
                     RSRLCEHAYQRTRSDLYRRRHELRPIFERHGIELNLGVLKDHSRSLLSKKRRPDLATS
                     ASALDSSLRDLEAWLLAQKA"
BASE COUNT          620 a          981 c          934 g          603 t
ORIGIN      
        1 gcccccgagc atggcggccc ggaggcagcg tcggaggaac cggatgacga tgacgccagt
       61 gacgtaccgg aaggcgacgc tttctggcgg accatcctcg atccactcac ggacgttgca
      121 ctggcgtcaa tccgcctggg tggccatctg tggggacagt ttctcgggct tcgcaatgac
      181 ccggcaggta tccgcgacta cgcgcgcgtc gcggcagaaa tcgcccagga agtcgggaag
      241 ctggccctgc tacccaacga cagtccaaca cgcttcaagg gaaagccggg aacggtcaaa
      301 cgcgtcgcct ggtcggaacc catagccttg tcggacatca aggccgtcgg caaggtcctc
      361 ggctgttcgg tcaacgatac cctgctggcg gcggtggccg gcgccttgcg cggctacctg
      421 gttgcgagag gcgatccggt ggtaagcacc gccgccatcc gcgccatggt ccccgtcaat
      481 ctgcgagcag cgagcgatgt cgatgaactc ggcaaccgct ttggcctggt cgccctcgaa
      541 ctaccgatcg gtatcgacaa tccgctggcc cggctgtacg ccacgcgtgc caggatggcg
      601 gcgttgaaga gttcctacca ggcgatgctg accttcgccc tgctcggtgc agccggcacc
      661 gccccgaagt tcgttcagga ccagatcctc aaactgctga ccagcaagac gactgcggtg
      721 atgaccaacg tacccggtgc acagcaaccc cgctacttag ccggcaacag gatcgaccag
      781 caaatggtct gggtaccgca ggccggcgac atcggcatgg gcgtcagcat cctctcctac
      841 aatggccgag tacaattcgg actgatcacc gacaagaacc gagtcgacga ccccgagcag
      901 atcgtgggcc gcttcgccga cgagttcgag aagctcttgt ggctgctcct gctggaaccc
      961 tgggatcgcc tgggggaccc gacggccgtg gaggaagacc tctcgcgcct gatcaagacg
     1021 gccgccgaat aggggtcggt agcgcagacg gcgttcagtc tgccaattcg accttggctg
     1081 cctcgacgtc gagacgctcc atggcgtcca tcggctggca ttccgcggct tcctggtaga
     1141 gccgctcggc atccttcatg ccggccttgc cgaacatcat caccagggca ttggcatact
     1201 cgatgcgggt gatcgcggaa tccgggttga gtttcagagc ggcctcaagc agggccttgc
     1261 cggcgtcttt tttgaccccg taagtaatgc cgccaaccag agccccgacc ttgtcgatca
     1321 cctcggcgtg atacgcgccc agcgcgatat gcgcgtcggc gtgcctgggt tcgatctcca
     1381 gcgcctgcgt caggctgtgt ttgatcttcc cacccagacc ttgtgcgagc gccttgacca
     1441 ccgagatgct ctggctgtaa cgaccgagag caaaggcatg cagataccag gcgttgacca
     1501 ccttgggcga tttctgctgc tgctcctcac agcgctgcgc aacctccatc agcaggtgac
     1561 gcttgcggtc gtcgtcggtc tcgaggtaac tggcatagat cgtggcagcc ttgttggcgg
     1621 cgttgtagcc ggcgagacca caagccagac cggcctcgac cgccttgccg aagtcacccg
     1681 catggtaact ccgccaggca tcctggacct tgggatcgtc aggaaacggc tcgcagtcgc
     1741 cacggtgcag acgaccccaa ttctcctgta gcgccgcacc ggcatagttg tagtcctggt
     1801 ccgggtaggg aaaggttttc caagtcatgc tcttcctcca tatgggttac gctctgaggt
     1861 attcatgcga cagtttttcg agcaaccctt tcgattcgcc aaacagatac ggcgagatca
     1921 tcgccatcac ctgatatacg ccgcggccga tgcgtccact ttcaaccttg ccgcgcggat
     1981 cacaagcgta ttcgaaggac agccagtagg ttgcgatcac cgtcatgttc gtcgccagtg
     2041 cgcgcagttc ctcagggcct gcgcgcatcg cacctgcgaa caccagactt tcgcacaagg
     2101 tcgtcgccgt acgcaccttg tgcgccagga tctgtttgaa atgaatctcc aatacccgat
     2161 tgcgtgtcag cagatcattc aggtcgcggt aaaaaaaacg atacttccag atctgttcga
     2221 acagcaggtg cagaaaaagc cagatgtctt cgacatcggc tgaccgccgc gtcggcaccg
     2281 cgaggatgtc ctcgatctcc cgctcgaagt cagcgaaaat cgactccagt atctcgtcct
     2341 tgttgtgaaa atggtaatag aggttgccgg ggctgatgtc catttcgtcg gcgatgaccg
     2401 tggtcgtcac gttcggctca ccaaaatcgt tgaacaggcg caggctggtt tccagaatac
     2461 gttctcgcgt tcgccgcttc ggtttctttt ccatcaactg tccaccgtga tccgctcgga
     2521 aaggccagaa tatcaccgcc ggcaacgtca gggaatggcc gacgatcgaa attgaaccga
     2581 cggccgaacc ggatcaggcc ttctgagcca gcagccaggc ttccagatcg cgcagcgaag
     2641 aatccagggc actggcgctg gtcgccaggt cgggacgacg tttcttcgac aacagggagc
     2701 ggctatggtc cttgagtaca ccaaggttca gctcgatccc gtggcgctcg aagatcggcc
     2761 tcaactcatg acgccgccga tacagatcgc tgcgcgtacg ctgataggca tgctcgcaca
     2821 agcggctgcg gccgcgataa ctgaatacgt tggtgaagaa catgtccgca tcgtcgcggg
     2881 tgggttcgaa cagcaccaca tccttgccct cgaattcaag acggtagcgg tccatgccgg
     2941 tgcgcatccg cgagtggatg atggcgcgga aagtctgcga caggaccacc ggcagccccc
     3001 cgtcgacgag atgctcggga gccggagaag ggttctcgtg cgctggtcgt ttggccgcca
     3061 ggcgcgcatc gaatggcacc agcgggttga tgcagatgac cagttcggca ccttcgtgca
     3121 atgccacgga cgcgtgca
//
LOCUS       DAOSIT010000255         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27054_length_3128_cov_6.818418, whole genome shotgun sequence.
ACCESSION   DAOSIT010000255 DAOSIT010000000
VERSION     DAOSIT010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27054_length_3128_cov_6.818418"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLL14_06990"
     CDS             complement(<1..380)
                     /locus_tag="PLL14_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOG03709.1"
                     /translation="MSFQQGLSGLDASSAALDVIGNNVANASTVGFKSGSAHFADLFA
                     ASLGILGASQVGIGVDVAAIQQQFTQGNVTTTNNPLDLAINGAGFFRVSHAGATSYTR
                     NGQFHLDNQGYVINDQNLRLTGYTA"
     gene            complement(395..1084)
                     /locus_tag="PLL14_06995"
     CDS             complement(395..1084)
                     /locus_tag="PLL14_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOG03710.1"
                     /translation="MAAVQSSTAASDIFAALNAQAKGSSTVADVTSTSEAVQNRFLKL
                     LVTQLKNQDPLNPLDNAAVTTQISQINTVTGIEKLNATLETLLNTYNEGQAMQAAGLI
                     GKSVMVAGSKLALADGEAIGGVNLAGAADSVKLTILDAAGKVVQSQALGAREAGSFAF
                     NWDGKTDTGSIAPAGNYSFKVDAVRGGEKVVAEALQIGTVSALVKGKTGYQLELGGLG
                     RVDFDKVEQIL"
     gene            complement(1114..1518)
                     /gene="flgC"
                     /locus_tag="PLL14_07000"
     CDS             complement(1114..1518)
                     /gene="flgC"
                     /locus_tag="PLL14_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOG03711.1"
                     /translation="MSIFNTFHVAGSALTAQSMRLNAVASNLANADSVVSSDGKPYRA
                     KQVVFAATPMGSAAAQGVRVAQVVEDSSPARMVYDPRNPVADEKGYVAMPNVSVVDEM
                     TNMISASRSYQTNAEVMNTAKQLLLKTLTLGQ"
     gene            complement(1524..1925)
                     /gene="flgB"
                     /locus_tag="PLL14_07005"
     CDS             complement(1524..1925)
                     /gene="flgB"
                     /locus_tag="PLL14_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOG03712.1"
                     /translation="MVNKLDSAFFLQQQALTLRANRQQVLAGNIANADTPHYQARDFD
                     FASALQKAVSGGGEGSLRLAVTAAGHLPGSADSVPVRLMYRQVTQASADGNSVEMDVE
                     RAQFAENAIHYEAGMTFLSGKIKTLLAALQA"
     gene            2138..2842
                     /gene="flgA"
                     /locus_tag="PLL14_07010"
     CDS             2138..2842
                     /gene="flgA"
                     /locus_tag="PLL14_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOG03713.1"
                     /translation="MCIARTRKRPGRLLRLLALLSAAMSNTVDAQTSLPTALDHYLRI
                     QTQGLPGKVSYSIGKLDQHTQLAACSAFEPFLPPGSRLWGNATVGIRCLGPSAWTVYV
                     PVQVRIAGNYLLTARRLTPGQVVTASDVVTQSGDLGALPAGILTDPAQAIGKTVRNGV
                     AAGQPLRGDVLIAAWAVQQGQSVRLLTTGTGFSVSNEGKALNNATDGQIVQARTASGQ
                     VVSGIARNGGTVEVAY"
     gene            2839..>3128
                     /locus_tag="PLL14_07015"
     CDS             2839..>3128
                     /locus_tag="PLL14_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOG03714.1"
                     /translation="MSFRCGRKNNVKVLPDLADTWIEYNRSKGTTVKIDSTTTTASTV
                     SETRNRTTIAQPAAGAAAEVHLSALAAQLQAPTEAPTFDAGRVAEIRQAISEG"
BASE COUNT          610 a          970 c          906 g          642 t
ORIGIN      
        1 gccgtgtatc cggtcagacg aaggttctgg tcgttgatca cgtaaccctg gttgtcgagg
       61 tgaaactgac cattgcgggt gtagcttgtc gctccggcat ggctcacgcg aaaaaagccg
      121 gcgccattga tcgccagatc aagcgggttg ttggtggtcg taacgttccc ctgggtaaac
      181 tgttgctgga tcgcggcgac gtccacgcca atacccacct gcgaagcgcc aagaataccc
      241 agggacgcgg cgaacagatc cgcgaaatgt gcgctcccac tcttgaaacc gaccgtgctg
      301 gcgttcgcca cattgttgcc gatcacatcc agtgctgcgg acgaagcatc cagaccactc
      361 aaaccctgtt ggaaactcat attgctcctc gcttctaaag aatctgttca accttgtcaa
      421 aatctactct tcccagcccg ccgagttcaa gctgatagcc ggtcttccct ttgaccaacg
      481 cagaaaccgt accaatctgc aaggcttctg caaccacttt ttcaccgccg cgcacggcat
      541 caaccttgaa actgtagtta cccgccggcg cgatgctccc ggtatcggtc ttgccgtccc
      601 agttgaaggc aaagctgcct gcttcacgcg cacccagcgc ctgactttgc acgaccttgc
      661 cggcggcatc gagaatggtc agcttcacac tgtcggctgc cccggccaga ttgacgccgc
      721 caatggcttc accgtcggcc agtgccagct ttgacccggc caccatgacg ctcttgccga
      781 tcagacccgc agcctgcatc gcctgaccct cgttgtaggt gttcagcaag gtttccagcg
      841 tggcgttgag tttctcgata ccggtcaccg tattgatctg cgaaatctgg gtggttaccg
      901 cagcgttgtc gagggggttg aggggatcct ggttcttgag ttgcgtcacc agcaacttca
      961 gaaagcgatt ctgcacggcc tcactcgtcg aggtgacgtc tgcgaccgtg ctgctaccct
     1021 tggcctgagc attgagcgcg gcaaagatgt cactggctgc ggtgcttgat tgtacggctg
     1081 ccattggttt ctccttgatg ttcttttcta ggtctactgg ccgagggtca acgtcttcaa
     1141 cagcagttgt ttggccgtgt tcatcacttc ggcgttggtc tggtacgagc gcgaagccga
     1201 gatcatgttg gtcatttcgt cgaccacact cacgttgggc atggcgacgt agcctttttc
     1261 atccgcaacc ggattgcgtg ggtcataaac catgcgcgcc gggctgctgt cctcgaccac
     1321 ctgggccaca cggacccctt gtgccgcggc actgcccatc ggcgtggccg caaaaaccac
     1381 ctgtttggcg cgataaggtt tgccgtcgga actcaccaca ctgtccgcgt ttgccaggtt
     1441 gctggccacc gcgttgaggc gcatcgactg cgcggtcagc gcactgcccg ctacgtgaaa
     1501 ggtgttgaaa atactcatcg tgactacgcc tgtagtgcgg cgagcagggt cttgatcttg
     1561 ccgctgagga aagtcatccc ggcctcgtag tggatcgcgt tctcggcaaa ctgagcacgc
     1621 tcgacatcca tttcgacact gttgccgtcg gcactggcct gcgtgacctg gcgatacatc
     1681 aatcgcaccg gaaccgaatc cgcactcccg ggcaagtgcc ccgccgcggt gacagccagc
     1741 cgcaagctgc cttcaccgcc gccgctgacc gccttctgca gcgcgctggc aaagtcgaag
     1801 tctctggcct ggtaatgcgg ggtgtcggcg ttggcaatat tccccgccag cacctgctgc
     1861 cgattggcgc gcagggtaag cgcctgctgt tgcaggaaga atgcgctgtc aagcttgttg
     1921 accatgggaa cctcgccagg cgatttgatg caaaatacca agcaatactc gtgccgtggc
     1981 tcttttgcag caacaggcaa tgccgctgat gtccgctagc tgcagcaccg cgtcggtagt
     2041 tgccggactt ccggcaaata ttgccggcgg ccccaaagca tcgcctcaac cggacttcat
     2101 ccttcgccca gggcacagat tcaggcagaa tggcgggatg tgcattgctc gaacccgaaa
     2161 acgccctggc cgcctgctga gactgctggc cttgctgtcg gcagcgatga gcaatacggt
     2221 agacgcccag acgtcgttgc ccactgcact ggaccactac ctgcggatcc agacgcaagg
     2281 actgccgggc aaggtcagtt atagcatcgg caaacttgat caacataccc agcttgccgc
     2341 atgcagtgcg tttgaaccct tcctgccacc aggcagccgg ctctggggca acgcgacggt
     2401 cgggattcgt tgcctaggcc ccagtgcatg gaccgtttat gtacccgtgc aggtgcggat
     2461 tgcgggaaac tacctgctca ctgcgcgccg gctgacgccg ggccaggtcg tgaccgcgtc
     2521 cgatgtggtc acgcagagtg gtgatctcgg cgccctgccg gccggtatcc tgaccgaccc
     2581 ggctcaggcc attggcaaga cggtcaggaa cggcgtcgct gccggtcaac cgctacgcgg
     2641 cgacgtgctg attgccgcct gggctgttca gcagggacag agcgtcagac tgttgacgac
     2701 gggcaccggc ttcagtgtca gcaacgaggg aaaagcactg aacaacgcca ctgacgggca
     2761 gatcgttcag gcgcggacgg cctccggcca ggtggtcagc ggcatcgccc gtaacggtgg
     2821 cactgtagaa gtggcctatt gagctttcgc tgcggcagga agaataatgt taaagtttta
     2881 ccggacctcg ccgatacatg gattgagtac aaccgttcaa agggcacgac cgtgaagatc
     2941 gacagcacaa ccacaactgc gagcactgtc agcgagacgc gcaatcgcac aaccatcgcc
     3001 cagcctgcag caggcgctgc ggcggaagtc catctgagtg cgcttgccgc tcaactgcag
     3061 gcacccacgg aggcaccgac cttcgatgcc ggccgggtgg ctgaaatcag gcaagccatc
     3121 tccgaggg
//
LOCUS       DAOSIT010000256         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27209_length_3113_cov_12.639961, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000256 DAOSIT010000000
VERSION     DAOSIT010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27209_length_3113_cov_12.639961"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..550
                     /gene="rpsF"
                     /locus_tag="PLL14_07020"
     CDS             164..550
                     /gene="rpsF"
                     /locus_tag="PLL14_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG03715.1"
                     /translation="MRHYEIVLIVHPDQSEQVPAMVERYKTLVATRAGQIHRLEDWGR
                     RQLAYPIQKLHKAHYILMNIECDDATLTELEHGFKFNDAILRHLTIRTKRAVSTPSPM
                     MKDEKSKSLLEVREPAPAATAGQPAA"
     gene            565..894
                     /gene="priB"
                     /locus_tag="PLL14_07025"
     CDS             565..894
                     /gene="priB"
                     /locus_tag="PLL14_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HOG03716.1"
                     /translation="MNRVELTGTLIERKALRFTPAGVPVSECLVGHQSEQLEAGSPRR
                     VECEIQAIALGATAQWLQAASPGAALHLTGFLAARSRNSKQPRLHITTIEFVEGNQDG
                     QVLQEEG"
     gene            866..1141
                     /gene="rpsR"
                     /locus_tag="PLL14_07030"
     CDS             866..1141
                     /gene="rpsR"
                     /locus_tag="PLL14_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG03717.1"
                     /translation="MARFFKKKDDKNVKKRGSGLFKRRKFCRFTAEKMEEIDYKDVDL
                     FKEYIAENAKIMPARLTGTKAGYQRMLSVAIKRARFLALLPYTDNHQ"
     gene            1156..1614
                     /gene="rplI"
                     /locus_tag="PLL14_07035"
     CDS             1156..1614
                     /gene="rplI"
                     /locus_tag="PLL14_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG03718.1"
                     /translation="MQIILMEKVANLGNLGDLLKVKDGYARNFLIPKGKAKRATPAAL
                     KEFEVKRVELENAAAEKLAAAQAYAEKLGGVVVRIARKAGVDGRLFGSVTNFDVADGL
                     QALGFAIEKSAVRMPAGPLKMIGESQLQVALHSDVMATITVAVVVEQLKI"
     gene            1615..1743
                     /locus_tag="PLL14_07040"
     CDS             1615..1743
                     /locus_tag="PLL14_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03719.1"
                     /translation="MLDSASLSGAEEEALPAGKAFLRSLAGISFLGALNSQPNSFS"
     gene            1754..>3113
                     /gene="dnaB"
                     /locus_tag="PLL14_07045"
     CDS             1754..>3113
                     /gene="dnaB"
                     /locus_tag="PLL14_07045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG03720.1"
                     /translation="MVKPFDSFNSENVQRANGRGARRERNPQADPALSELRVPPHSIE
                     AEQSVLGGLLLDNAAFDKIADVMSEGDFYRDEHKRIYRQIRKLLERGKPADAVTVAES
                     LDQAGEGKDTGGLAYLGELAANTPSAANIRRYAEIVRERAILRQLVTAGDEIAGSALN
                     PLGRDPKTLLDEAEAKVFAIAEGGFRHQSGFVHINPLLTQVVERIQELHDRDNPSDIT
                     GVPSGYHDLDSKTSGLQGGDLLIVAGRPSMGKTSFALNIAEHVAIEVGLPVAIFSMEM
                     GGTQLAMRMLSSVGRLDAHRVRTGRLNDDEWSRLSFALGKMHEAPIYIDETPALNPID
                     LRARARRLHRQCGKLGLIVIDYLQLMSATSHGENRATEISEISRSLKGLAKELGVPLM
                     ALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVIMFIYRDEVYNPDTPDKGVAEIII
                     GKQ"
BASE COUNT          647 a          920 c          974 g          572 t
ORIGIN      
        1 tgcggggcta caggcaaacg aacccgattg ggcagggtca tgcgtcaggg ctgcaaccct
       61 ggcgaaagcg ttgctcgaca gacggcaagg gggctataat acgcggtttt tccaccttgc
      121 cccaccgttc gcatggcggg gggttaaccc ataaggagtg tgcatgcgcc actatgaaat
      181 cgttttaatc gtccacccgg accagagtga gcaggtcccg gcgatggtcg agcgctacaa
      241 gacacttgtt gccacacgcg cagggcagat ccaccgtctc gaagactggg gacgccgcca
      301 actcgcctat ccgatccaga agttgcacaa ggcgcactac attctgatga acatcgagtg
      361 cgatgacgcc acgctgaccg aactcgaaca cggcttcaag ttcaacgacg ccattcttcg
      421 tcatctgacg atacgcacca agcgtgccgt cagtacgccg tcgccgatga tgaaggatga
      481 gaagtccaag tcgctgctgg aagtccgtga gccggcgccg gctgcgactg ccggacagcc
      541 agccgcctga ttgacgaggc gctgctgaac cgcgtcgagc tcaccggaac cctgatcgag
      601 cgcaaggcgc tgcgttttac accggctggt gtgccggtca gcgaatgcct ggtcgggcat
      661 cagtcagagc agcttgaagc aggcagtcca cgccgtgtcg agtgtgaaat ccaggcgatt
      721 gccctgggtg cgacggcaca gtggttgcag gcggcaagcc cgggggcggc gctgcatctg
      781 acgggttttc ttgccgccag gagccggaac agcaagcagc ccaggctgca tatcactacg
      841 atcgaatttg tcgaaggaaa tcaagatggc caggttcttc aagaagaagg atgacaagaa
      901 tgtcaagaag cgcggcagtg gtttgttcaa gcggcgcaag ttctgccgct tcactgccga
      961 gaagatggaa gagatcgact acaaggatgt cgacctcttc aaggagtaca tcgctgaaaa
     1021 cgccaagatc atgccggcgc gtctgaccgg caccaaggcc ggttatcagc gcatgctgtc
     1081 ggtggccatc aagcgggcgc gcttcctggc gctgctgccg tataccgaca atcaccagta
     1141 agcgaggaca tgacgatgca aatcattctg atggaaaagg tcgccaacct cgggaatctc
     1201 ggcgacctgc tcaaggtgaa agatggttac gcgcgcaact tcctgatccc gaagggcaag
     1261 gccaaacgtg cgacgccagc ggccctgaag gagttcgaag tcaagcgagt cgaactcgag
     1321 aacgcggccg ccgagaaact ggcggctgcg caggcctatg ccgaaaaact tggcggcgtc
     1381 gtggtcagga tcgcgcgcaa ggctggcgtg gatggccgtc tgtttggctc ggtcaccaat
     1441 ttcgatgtcg cggatggcct gcaggcgctc ggttttgcca tcgaaaagtc tgccgtgcgc
     1501 atgcccgccg gtccgctcaa gatgatcggt gagagccagt tgcaagtggc cctgcacagc
     1561 gacgtcatgg cgacgattac ggtggccgtg gtcgtcgaac agctgaagat ttaggtgctg
     1621 gattcggcgt cgctttccgg cgccgaagag gaagccttgc ccgcgggcaa ggcttttttg
     1681 cgttcgcttg ccggtatctc gttcctgggc gcgctaaact cgcagcccaa ctcgttctcc
     1741 tgacggatta ttcatggtca agcccttcga ttccttcaac tccgagaatg tgcaacgtgc
     1801 gaacggtcgt ggcgcgcggc gcgaaaggaa tccgcaggct gaccccgcgc tgtccgaact
     1861 acgcgtgccg ccgcattcga tcgaggccga gcagtcggta ctcggcggct tgttgctcga
     1921 caacgctgcc ttcgacaaga tcgccgatgt gatgtcggaa ggcgatttct atcgggacga
     1981 gcacaagcgc atctatcgcc agatccgtaa actgctcgaa cgcggcaaac cagccgacgc
     2041 ggtgacggtc gccgaaagcc tggaccaggc cggggagggc aaggataccg gcgggctggc
     2101 ctatctcggc gaacttgcgg ccaacacccc gtcggcggcg aatatccggc gctacgcgga
     2161 gatcgttcgc gaacgggcga ttctgcgcca actggtgacc gccggcgacg agatcgccgg
     2221 cagtgcactc aatccgctcg gacgcgatcc gaaaaccctg ctcgatgaag ccgaggcgaa
     2281 ggtctttgcg attgccgaag gtggctttcg ccatcagagc ggcttcgtgc acatcaaccc
     2341 gctgttgacc caggtggtcg agcgcatcca ggaactacac gatcgcgaca atccctcgga
     2401 cattaccggc gtgccgagcg gttatcacga tctggactcg aaaacctcgg gcctgcaggg
     2461 cggtgatctg ttgatcgtcg ccggccggcc gtcgatgggg aagacctcgt ttgcgctcaa
     2521 tattgccgag cacgtggcga tcgaagtcgg tttgccggta gccatttttt cgatggagat
     2581 gggcggtacc caactcgcca tgcgcatgct gtcctcggtc ggccggctcg atgcgcatcg
     2641 cgtccgcacc ggccgcctca atgatgacga gtggtcgcgc ctgtcctttg ccctgggcaa
     2701 gatgcatgag gcgccaatct acatcgacga gacgccggca ctcaacccga tcgatctccg
     2761 ggcgcgggcc cgccgtctgc accgccagtg cggcaaactc gggttgatcg tcatcgacta
     2821 tctgcagttg atgtccgcga cctcgcacgg cgagaaccgg gccaccgaaa tttccgagat
     2881 ttcgcgttcg ctgaaaggac tggcaaagga gttgggtgtg cccttgatgg cgctctcgca
     2941 actcaaccgc tcgctggaac aacggccgaa caagcggccg gtgatgtcgg acctgcgcga
     3001 gtcgggagcc atcgagcagg acgccgatgt gatcatgttc atctatcgtg acgaggtcta
     3061 caacccggac acgccggaca aaggcgttgc cgagatcatc atcggcaagc agc
//
LOCUS       DAOSIT010000257         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27223_length_3112_cov_7.213608, whole genome shotgun sequence.
ACCESSION   DAOSIT010000257 DAOSIT010000000
VERSION     DAOSIT010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27223_length_3112_cov_7.213608"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLL14_07050"
     CDS             complement(<1..293)
                     /locus_tag="PLL14_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03721.1"
                     /translation="MNGRSRPSSSRQRHWLGFMLLGVVLLAVPFVAALGGQAWVRIAN
                     FAILYVFLALGLNIVVGYAGLLDLGYVAFYAVGAYTYALLASPHFGIHLPFWV"
     gene            complement(290..1213)
                     /locus_tag="PLL14_07055"
     CDS             complement(290..1213)
                     /locus_tag="PLL14_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG03722.1"
                     /translation="MDTLLQQLINGLTLGAVYALVALGYTMVYGIIGLINFAHGEVVM
                     IGTMVAFTVIVSLAGSGMPPLLVLGIGLLVSVVVCMVLGWALERVAYRPLRHAPRLTP
                     LITAIGMSIVLQNVAMIIWGRNYITFPPMIHKQIYDIGGASFTNVQVAIVSLAAVVML
                     GLIYLVHRTRIGMAMRATAQNPHVASLMGVDINRVIAMAFIIGSALGAVAGCMVGSYY
                     EIAHYQMGFMLGLKAFTAAVLGGIGNLAGAMAGGLLLGVIEALGAGYIGDMTGDILGS
                     HYQDIFAFIVLIIVLIFRPSGLFGERTGDRA"
     gene            complement(1383..2684)
                     /locus_tag="PLL14_07060"
     CDS             complement(1383..2684)
                     /locus_tag="PLL14_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG03723.1"
                     /translation="MKSVRTPGVLLVLAASLAVIGCGKKEEPKAAAPAPAPVAAPAPA
                     PKPEMVVKIGHVAPLTGPIAHLGKDNENGARLAVDDANAKKIEIDGKVIKFELLGEDD
                     QADPKTGTVVAQKLVDAKVAGVVGHLNSGTTIPASAIYNAAGLPQISPSATNPKYTQQ
                     GFNTAFRVMANDIQQGSVVGAYAVKDLGAKKIAIIDDRTAYGQGLADEVEKAAKAAGA
                     TIVAREFTNDKATDFKAILTKIKAKKPDVIFLGGMDAVGGPMLKQMKELGITAKFTTG
                     DGGCSPELIKLAGPASEGVVCTQAGLPVDKLPNGQKFVEDFSKKYGQIQIYSPYSYDA
                     VMVLVDAMKRANSADPVKYLPEVGKTSYDGVTGRIEFDEKGDIKNGAITVFEIKDGKL
                     VTLKSEGGTAPAAPAAAAAPAPAPAAPAPAAAPAPAPEAKK"
     gene            complement(2856..>3112)
                     /locus_tag="PLL14_07065"
     CDS             complement(2856..>3112)
                     /locus_tag="PLL14_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOG03724.1"
                     /translation="GAVQADEALAKSKNCLSCHAVDKKVVGPAYKDVAKKYTAKDEAM
                     LAAKVIKGGKGSWGEVPMPPNPSVTPDEANKLVKWILSLK"
BASE COUNT          653 a          946 c          928 g          585 t
ORIGIN      
        1 atcacccaga acggcaggtg aatgccgaaa tgcggcgacg ccaggagtgc gtaggtatag
       61 gcgccgacgg cgtagaaagc gacgtaaccg agatcaagca gacccgcata gccgacgacg
      121 atattcagac ccagtgcgag gaagacgtaa agaatcgcga aattcgcaat ccgcacccac
      181 gcctgccccc ccagcgcggc aacaaaagga acggccagca gcacgacgcc gagcagcatg
      241 aagcccagcc aatgtctctg ccgagaggaa gatggtctgg atcggccgtt catgcgcgat
      301 ctcctgtacg ctcgccaaaa agacccgaag gacgaaaaat cagaacgatg atcaacacga
      361 tgaaggcaaa aatgtcctga tagtgactgc cgaggatgtc accggtcata tcaccgatgt
      421 aaccggcacc cagcgcctcg atcacaccga gcaacaggcc acccgccatg gctcccgcga
      481 gattgccgat gcctcccagg acagccgccg tgaaagcctt caggccaagc atgaaaccca
      541 tctggtaatg cgcaatctcg taatacgaac cgaccatgca gccggcgacg gcgcccagcg
      601 cggagccgat gatgaaggcc atggcgatga cgcggttgat gtcgacgccc atcagcgacg
      661 ccacgtgcgg gttctgtgcc gtagcccgca tggccatgcc gatgcgcgtg cgatgaacca
      721 ggtaaatcag acccaacatc accaccgccg ccagtgacac gatggcgacc tgaacgttgg
      781 tgaaactggc gccgccgatg tcgtagatct gtttgtgaat catcggtgga aaagtgatgt
      841 agttccggcc ccagatgatc atcgccacat tctgcagcac gatcgacatg ccgatcgccg
      901 tgatcagcgg cgtcagtctt ggcgcatgcc gaagggggcg ataggcaaca cgttcgagtg
      961 cccagccgag caccatgcag accaccaccg aaaccagcaa gccgatgccg agaaccagca
     1021 gaggcggcat accggagccg gcaagggaga cgatcaccgt aaaggcgacc atcgtaccga
     1081 tcatcaccac ctcaccgtgg gcgaagttga tcaggccgat aatgccatac accatcgtgt
     1141 aacccagagc gacgagcgcg tagacagcgc ccagcgtgag gccgttgatc agttgctgga
     1201 ggagagtgtc cataatcgag ctttccgcaa caaggtgctg ctggcgaacc gtatgttccg
     1261 aggcatgccg ccagggaggc ggccgcgcgc aagcaggacc cggttgatgc tgagcggatg
     1321 cggatgcagg aaaaacgagg gaatggccgg agccattccc tcgggatcaa gttgccgtgc
     1381 ccttacttct tggcttccgg agccggtgcg ggtgcagcag caggtgccgg ggccgcaggt
     1441 gctggcgccg gggcagctgc agcagcgggc gcagccggtg ccgtaccgcc ttcggatttc
     1501 agggtaacca gcttgccatc cttgatttcg aaaacggtaa tcgcgccgtt cttgatgtca
     1561 cccttctcgt cgaactcgat cctgccggtg acgccatcgt aggaggtctt gccaacttcc
     1621 ggcagatact tgaccgggtc ggccgagttg gcacgcttca tggcatcgac caggaccatc
     1681 actgcgtcat agctgtaggg agagtagatc tgaatctgac cgtacttctt ggagaagtct
     1741 tcaacaaact tctggccgtt cgggagtttg tcgaccggca ggccggcctg cgtacaaacc
     1801 acgccctcgg acgccgggcc ggcgagcttg atcagttcgg gcgagcagcc accgtcgccg
     1861 gtggtgaact tggcggtgat gccgagttcc ttcatctgct tgagcatcgg accacccacc
     1921 gcatccatcc cgccaaggaa gatcacgtca ggcttcttgg ccttgatctt ggtcagaatg
     1981 gccttgaagt cggtcgcctt gtcgttggta aactcgcgcg cgacgattgt cgcgccggca
     2041 gccttggcag ccttctcgac ctcatccgcc aaaccctggc cataggccgt gcgatcgtcg
     2101 atgatcgcaa tcttcttggc gcccaggtcc ttcaccgcgt aggcgccgac caccgagccc
     2161 tgctggatgt cattggccat cacccggaag gcggtattga agccctgctg ggtgtacttc
     2221 ggattggttg ccgagggcga aatctgcggc aggccggccg cgttgtagat cgccgaggca
     2281 gggatggtcg tacccgagtt gaggtgcccg acgacgccgg ccactttggc atcaaccagc
     2341 ttctgggcga cgacggtacc ggttttcggg tcggcctgat catcttcgcc aagcaactca
     2401 aacttgatga ctttgccatc gatctcgatc ttcttcgcgt tggcatcgtc gacggccagg
     2461 cgggcaccgt tctcgttgtc cttgcccagg tgcgcgatcg ggccggtcag tggcgcgaca
     2521 tggccgatct tgaccaccat ttcgggtttc ggcgccggcg cgggcgctgc caccggggca
     2581 ggagcagggg ccgcagcctt gggttcctct ttcttgccgc agccgatcac ggccagcgag
     2641 gctgccagaa cgagcaatac accgggtgta cgaacactct tcataggttt catctccgat
     2701 attgtctgtg tagagggtgg gaaacgggcg attgtccaag cacatcagga ccttgtcaac
     2761 atgcatatga tctctcgacc tgcatatcag ctttcggcac cacatccctg aaacggaaaa
     2821 ccggcaattg gccggttttt ttgttcatgc aacgatcact tcagactgag gatccacttg
     2881 accagcttgt tcgcctcgtc gggcgttacc gaaggatttg gcggcatcgg cacttcaccc
     2941 caggagccct tgccaccctt gatgaccttc gcagcgagca tggcttcatc cttggcggtg
     3001 tacttcttgg ccacgtcctt gtaggccggc ccaacgacct tcttgtcgac ggcgtgacaa
     3061 gacaggcagt tcttggactt ggccagggct tcatcagcct ggacggcgcc gg
//
LOCUS       DAOSIT010000258         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27407_length_3096_cov_10.521539, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000258 DAOSIT010000000
VERSION     DAOSIT010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27407_length_3096_cov_10.521539"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLL14_07070"
     CDS             complement(<1..332)
                     /locus_tag="PLL14_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOG03725.1"
                     /translation="MKLVLASNNTKKLKELDAILAPLGWELVPQGQLGVPEAEEPHCT
                     FVENALTKARHASRLSGLPALADDSGLCVDAFGGAPGVLSARYAGEPKSDARNNQKLL
                     VELGENDRR"
     gene            complement(329..1045)
                     /gene="rph"
                     /locus_tag="PLL14_07075"
     CDS             complement(329..1045)
                     /gene="rph"
                     /locus_tag="PLL14_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOG03726.1"
                     /translation="MRPSNREPSQLRQVRMTRRFTCHAEGSVLIEIGNTRVLCTASVE
                     EFVPAFLRGSGQGWVTAEYGMLPRSTHTRTGREAAKGKQTGRTQEIQRLIGRALRAVT
                     DLKALGERQITLDCDVLQADGGTRCAAITGAWVALNDACEHLLGKRKIPAHPVRDQVA
                     AVSVGIYQGLAVVDLDYAEDAHCDTDMNVVMTGRRGIVELQGTAEGEPFTRTQMNQLL
                     DLAEAGIGELLAHQKTALTL"
     gene            complement(1054..1974)
                     /locus_tag="PLL14_07080"
     CDS             complement(1054..1974)
                     /locus_tag="PLL14_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HOG03727.1"
                     /translation="MKFTIYQESRTGKRANNEDRLAYCYSREALLMVVADGMGGHYYG
                     EVAAQIAVQTLTEAFPQAFKRKVLDPFMFLQENMLNAHRGILDFAGKHHLLDSPRTTC
                     VACIVQNSVAYWAHAGDSRLYLLRNGKVLAQTRDHSRVRMLVDQGKISEAEVTGHPDR
                     NKIYGCLGGQQAPQIEYSRKTTLETGDVLLLCTDGVWSVLPGDSLAGSLKTADLMRAV
                     PALLDQAEERGGPHADNLSMIAVRWGDTYLDESSSSSSLISTKTMARDTVMTKLDEFG
                     RHPARKSELTEQEIELAIEEIRSTIQKYSK"
     gene            complement(1971..2930)
                     /locus_tag="PLL14_07085"
     CDS             complement(1971..2930)
                     /locus_tag="PLL14_07085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOG03728.1"
                     /translation="MAQQANHPLPAGFRIDEYCIEHQLSLGGFSIVYVATDAHGQRVA
                     IKEYLPNTLALRSEGQTAPIITEDHLPAFRYGMKCFFEEGRALAGLSHPNVIRVLNFF
                     RANDTVYMVMEYEHGRTLQELIQKNRPGVTENFIRNIFTKMLNGLREVHSHRLLHLDL
                     KPSNIYMRNNHMPVLIDFGAARQTLACDTPMLKPMYTPGFASPEHYHQRELLGPWSDI
                     YSVGASMYACISGNTPQPADNRLEKDRLVPAMVRWEGQYSDQLLETIDWCLCLNHRYR
                     PQSVFALQKALTEAVVPPTPSPTEPKETWLDQVVSKFKKTRDA"
BASE COUNT          532 a          890 c          999 g          675 t
ORIGIN      
        1 cgcctgtcgt tttcacccag ttcgacgagc agcttctggt tgttgcgggc atcggatttc
       61 ggctcgcccg cgtagcgtgc ggataacacg ccgggggcgc cgccgaaggc gtcgacacac
      121 agacccgagt cgtcggcgag ggccggcagg ccgctcagcc gcgacgcgtg ccgcgccttg
      181 gtcagggcat tctcgacgaa ggtacagtgc ggctcctcgg cctcgggaac gccgagctgg
      241 ccctggggaa caagctccca gcccagcggc gcgagaatcg catcgagttc tttcagtttc
      301 ttggtgttgt tggaagcgag aacgagtttc atagggtcag ggccgtcttc tggtgggcca
      361 gcagttcacc gatgccggct tctgccaggt ccagcaactg gttcatttgc gtacgagtaa
      421 agggttcgcc ttcggcggtt ccctgcagtt cgactattcc ccgcctgccg gtcatcacca
      481 cgttcatgtc cgtatcgcag tgggcgtctt cggcgtagtc gaggtcaacc acagccaacc
      541 cctgatagat tcccaccgac actgcggcga cctgatcgcg gacggggtgt gcagggatct
      601 tgcgtttgcc gagcaagtgc tcgcaggcgt cgttgagtgc cacccaggcg ccggtgatcg
      661 cggcgcagcg ggtgccgccg tcggcttgca ggacgtcgca gtcgagcgtg atctggcgtt
      721 cgcccaatgc cttgaggtcg gtcacggcac gcagggcgcg accgatcagg cgctggattt
      781 cctgggtgcg gccggtctgc ttgcctttgg ccgcctcgcg cccggtgcgc gtgtgcgtcg
      841 agcgcggcag catgccgtat tcggccgtca cccagccctg tccgctgccg cgcagaaagg
      901 ctggcacgaa ttcctcgacg ctggccgtgc agagcacgcg ggtgttgccg atttcgatca
      961 gtaccgagcc ttcggcgtgg cacgtaaagc ggcgcgtcat ccggacctga cgcaactgcg
     1021 atggttcacg gttgcttgga cgcatgtggt tccctatttt gagtatttct ggatggtcga
     1081 gcggatttct tcgatggcca gctcgatctc ctgctcggtg agttcgctct tgcgcgcggg
     1141 gtgacgaccg aactcgtcga gtttggtcat caccgtgtcg cgagccatgg ttttcgtcga
     1201 gatcaggctg ctggaactgc tgctttcatc gaggtaggtg tctccccagc gcaccgcgat
     1261 catcgacaga ttgtccgcat gcgggccgcc gcgttcttcc gcctgatcaa gcagtgccgg
     1321 aaccgcgcgc atcaggtcgg cggtcttcaa cgagccagcc aagctgtcgc caggcagaac
     1381 gctccagaca ccgtcggtgc acagcagcaa gacgtctccc gtctccagtg tcgtcttgcg
     1441 tgaatactcg atctgcggcg cctgctgacc gcccaggcac ccgtagatct tgttgcggtc
     1501 ggggtgaccg gtcacctcag cttcactgat ctttccctgg tcgaccaaca tccgtacacg
     1561 cgagtgatcc ctggtctggg ccaggacttt gccattgcgc aggaggtaca ggcgagaatc
     1621 accggcatgc gcccagtaag cgacgctgtt ctgcacgatg caggcgacgc aggtggtgcg
     1681 tggtgagtcc agaagatggt gcttgccggc aaagtcgaga atgccacggt gggcgttgag
     1741 catgttctcc tggagaaaca tgaaggggtc gagtaccttg cgcttgaagg cttgcgggaa
     1801 agcttcggtg agcgtctgca cggcgatctg ggcggcgact tcgccgtagt agtgaccgcc
     1861 catgccgtct gcgacgacca tcagcaaggc ttcgcgggaa tagcagtagg ccagccggtc
     1921 ttcattgttg gcgcgtttgc cggtccgact ttcctggtag atggtgaatt tcatgcgtcc
     1981 ctggttttct tgaatttgct gacgacctgg tctaaccagg tttccttggg ttctgtgggg
     2041 gagggtgttg gcgggacgac ggcttcggtc agtgccttct gcagggcaaa aacgctctgc
     2101 ggccggtagc ggtggttcag gcacaggcac cagtcgatcg tttcgagcaa ctggtccgag
     2161 tattgtcctt cccagcggac catggcgggt accagccggt ctttttcgag gcggttgtcg
     2221 gccggttgtg gcgtgttgcc cgaaatgcag gcatacatcg aggcgccgac gctgtagatg
     2281 tcgctccagg ggcccagcaa ctcgcgctga tgatagtgct cgggcgacgc gaagcccggg
     2341 gtatacatcg gcttgagcat cggtgtgtcg caagccagcg tctggcgggc cgcgccgaaa
     2401 tcgatcagca ccggcatgtg gttattgcgc atgtagatgt tagatggttt gaggtcaaga
     2461 tgcagcagcc ggtgtgaatg cacttcgcgc agaccgttga gcatcttggt aaagatgttg
     2521 cgaatgaagt tttccgttac ccccggcctg tttttctgga tcagctcctg cagggtgcgg
     2581 ccatgttcat attccatcac catgtaaacg gtgtcgttgg cacggaagaa gttcagcacc
     2641 cgaatcacgt tgggatggga aaggccggcc agcgcccgtc cttcctcgaa aaaacacttc
     2701 atgccgtagc gaaaggcagg cagatgatct tcggtgatga tcggcgcggt ctgcccttca
     2761 ctacgcaagg ccagcgtatt gggcaggtac tccttgatgg ccacacgctg cccatgggcg
     2821 tcggtcgcga cgtagacgat cgaaaaccca cccagcgaga gctgatgctc aatgcaatat
     2881 tcgtcgatcc ggaaaccggc ggggaggggg tggttcgctt gttgcgccat ccgtgtcagg
     2941 gctatcattc aaggtttcag gcattgtccg gtttttcggg gtggactgta aagccggcaa
     3001 ctgatacaca atcgtcaata tgtcaggatt tcgatgattt acagcatgac cggctatgcc
     3061 gccaggacgc gcgccgtcaa gggcggtgcc ttgcac
//
LOCUS       DAOSIT010000259         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27453_length_3093_cov_8.474984, whole genome shotgun sequence.
ACCESSION   DAOSIT010000259 DAOSIT010000000
VERSION     DAOSIT010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27453_length_3093_cov_8.474984"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1533
                     /locus_tag="PLL14_07090"
     CDS             19..>1533
                     /locus_tag="PLL14_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG03729.1"
                     /translation="MSTVIARHLLLVVLLALGAVANAADIPARPRVGLVLGGGGARGA
                     AHIGVLEVLERLRVPVDCVAGTSMGGLVAGAYAAGLSPTVMREELAKADWDDLFHDDP
                     SFSQQNFRKKVLDKRFLPASETGVGPDGLKYQTGVVTGQKIKLFFNQLVGDYLGEREI
                     DRLPLPLSIIATDIVQGQRVVFRSGSLTTAMRASMSVPGLMSPVEINGEKLVDGGLVD
                     NVPIGEARSRCQADVVIAVNVGSPLLKAEDISGLLSISVQMVNILTEQNVTRSLATLK
                     ATDIYIKPELEGISAGDFKRTSETADRGVKAAESVADRLRLLAVSEAAYTAWAESIQV
                     ARREAPKIHEIQIAGLRLVNPAMVEQHIQVKPGDPVNPSVINPDLLRVYGDGYYQNVD
                     YAMLDTLRDRNILRVMPLEKSWGPDYIRYGINLDSNFKSDSSYSLRLGYQKTWLNSLG
                     AELLAIGEIGSTNGVAVDFYQPVDARQRYFLETNLKYVSKISSIYQNDNKLAEYR"
     assembly_gap    1534..1633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1634..2075
                     /locus_tag="PLL14_07095"
     CDS             <1634..2075
                     /locus_tag="PLL14_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HOG03730.1"
                     /translation="SKVDGYASAYRSFGDWVLSSRVSYQGSPVGRLPFYDVGSLGGML
                     NMTAFAVGQLKGDDMSYVNARAERIIGRLPLGLRGDMRMGLAVEGAKIGTPYTETNLK
                     GWINSTAIYLGGETPLGPVYLGYGYSSSGSTGGYSNLYLFLGTP"
     gene            complement(2176..2784)
                     /locus_tag="PLL14_07100"
     CDS             complement(2176..2784)
                     /locus_tag="PLL14_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOG03731.1"
                     /translation="MKRPVLVLAMAAAMLLAAPVGHAAIISLYADLNGANEAPPNASP
                     GSGFASVSVDTGTHIMHVRVDFKDLLGTTTAAHIHCCTPLPLTATAGVATTVPNFIGF
                     PLGVTSGLYEHDFDLELDSSYNPSFLTAHGGTAAAAEAFLLAGMTSGRSYLNIHTTFR
                     PGGEIRGFLVPEPAMISLLTLGLGLGFVSLRRKNTGEDWRAA"
BASE COUNT          612 a          921 c          901 g          559 t
ORIGIN      
        1 cctgaatgga gtagcctcat gagcaccgtg attgcccggc acctgctgct ggttgttctg
       61 cttgcgctgg gtgcagtggc caacgccgca gatattccgg cccggccgcg tgtcggactg
      121 gtcctcggtg gcggcggcgc gcgcggcgcg gcgcatatcg gcgtgctcga agtgctcgaa
      181 aggctgcgcg taccggttga ctgcgtcgcc ggcaccagca tgggcggact ggttgccggc
      241 gcctacgcgg ccgggctctc gccgacagtc atgcgggaag aactggcgaa agccgactgg
      301 gacgacctgt tccacgatga cccgtccttc tcgcagcaga atttccgcaa gaaggtcttg
      361 gacaagcgct tcctgccggc gtcggaaacc ggcgtcggcc cggatggcct gaagtaccaa
      421 acgggcgtcg tcaccggcca gaagatcaag ttgtttttca accaactggt cggcgactat
      481 ctgggggaac gggagatcga ccgactgccg ttgccgctgt cgatcatcgc caccgatatc
      541 gttcagggcc agcgggtggt ctttcgcagc ggcagcctga ccaccgccat gcgcgccagc
      601 atgtcggttc ccggcctgat gtcgccggtc gagatcaacg gcgaaaaact ggtagacggc
      661 ggcctcgtcg ataacgtgcc tatcggcgag gcgcgttccc gctgtcaggc cgatgtcgtg
      721 attgccgtca atgtcggctc gccgctgctc aaggctgaag acatcagcgg cctgctgagc
      781 atttcggtgc agatggtgaa tatcctgacc gagcagaatg tgacccgttc actggccacc
      841 ctgaaagcga ccgacattta tatcaagccc gagcttgagg ggatcagcgc cggtgacttc
      901 aaacgcactt ccgaaactgc ggatcgtggc gtgaaggctg ctgaatcggt cgccgatcgc
      961 ctgcgcctgc tggccgtcag cgaagccgcc tacaccgcct gggccgagag catccaggtg
     1021 gcacggcgcg aagcgccgaa gatccacgaa atccagatcg ccggactcag actggttaac
     1081 ccagccatgg ttgaacagca catccaggtc aagccgggcg acccggtcaa cccgtccgtg
     1141 atcaaccccg acctgctgcg agtctacggc gatggctact atcagaacgt cgattacgcc
     1201 atgctcgaca cgctgcgcga ccgcaacatt ctgcgcgtga tgcccctcga aaaaagctgg
     1261 ggacccgatt acattcgcta cggtatcaac ctcgacagca atttcaagag cgactccagt
     1321 tacagtctgc gtcttggcta ccaaaagacc tggctcaact cgctgggtgc ggaactcctg
     1381 gccattggcg agatcggctc gaccaacgga gttgccgtag acttttacca gccggttgat
     1441 gcgcgccagc gctactttct cgaaaccaac ctgaagtacg tcagcaaaat cagcagcatt
     1501 tatcagaacg acaacaaact tgccgaatac cgannnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnncagcaag gtcgacggtt atgccagcgc ctaccggagc tttggcgact
     1681 gggtactgag cagccgggtt tcgtatcagg gctcgcctgt cggccgcctg ccattctatg
     1741 atgtaggaag cctcggcggc atgctgaaca tgaccgcgtt tgctgtcggt cagctcaagg
     1801 gcgatgacat gagctacgtc aatgcccgtg ccgagcgcat cattggccgc ctgccgctcg
     1861 gtctgcgtgg cgacatgcgc atgggtctgg cggtcgaggg ggccaaaatc ggcaccccgt
     1921 acacggaaac caacctgaaa ggctggatca actccaccgc catttatctc ggcggcgaga
     1981 ctcccctggg gccggtgtac cttggatacg gctactccag ctcgggttcg accggcggtt
     2041 actccaatct ctacctgttt ctcggaaccc cctgaccaga cctcccacct ctcagatcaa
     2101 acgcacaagc tgcttgccga gattggtgcc cttgagcagg ccgatgagaa ggcctcggga
     2161 accagaccag cggattcacg cggcccgcca atcttctccg gtgttcttgc ggcgcagcga
     2221 aacgaaaccc agtccaagcc caagagtgag cagactgatc atcgccggct ccggtaccag
     2281 gaagccgcgg atttcacctc ccgggcggaa agtcgtatga atgttcaggt aactcctgcc
     2341 cgaggtcatg ccggccaaga ggaacgcttc ggcggctgcc gccgtcccgc cgtgcgccgt
     2401 gagaaaactc gggttatagg aagagtccag ttccaggtcg aagtcgtgtt cgtacagccc
     2461 gctggtgacg ccgagcggaa aaccgatgaa attcggcacc gtcgtcgcca cacccgcagt
     2521 cgccgtcaaa ggcaagggcg tgcaacagtg aatgtgtgca gccgtcgtgg tgcccagcag
     2581 atccttgaaa tcgacgcgca cgtgcatgat atgggttccc gtatcgacgc tgacgctggc
     2641 aaatccggat cccggcgagg cgttcggtgg agcctcgttg gcgccgttaa gatcggcgta
     2701 aagactgatg atggccgcgt gacccacggg agctgcgagg agcatggctg cagccatagc
     2761 caagacaagg actggacgtt tcatgatgcc tcctgattgg tgaaggtgaa aagccagatt
     2821 gcgccgccgc gcgagcacgc cttacagcgc ccattcgcac gcctccaggc tgcacgcaaa
     2881 gatcatgccg atcatttata tagccattgc aacaatgggt tgcatgacat ccatggtgac
     2941 cacggcgacc acgtgtaaag attttcgaca cctgttacgc ggcttcacca ctggacaagg
     3001 ttgctgcgtc gctgcggctg cacgggtgcc cttgagggat cagctgatca aggtgtgtcc
     3061 cacagcgcgt gccaggattc cgacagcgga ccc
//
LOCUS       DAOSIT010000260         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27578_length_3083_cov_10.047226, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000260 DAOSIT010000000
VERSION     DAOSIT010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27578_length_3083_cov_10.047226"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..>3083
                     /locus_tag="PLL14_07105"
     CDS             391..>3083
                     /locus_tag="PLL14_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOG03732.1"
                     /translation="MNSAIPEGQGLYDPANEHDACGVGFVAHIRGKKSHSIIEQGLLI
                     LKNLDHRGAVGADPLMGDGAGILIQIPDAYLREEMAKQGVQLPQPGDYGVGMVFLPKE
                     SASRLACQEEIERAVRSEHQVVLGWRDVPIDHALPMSPTVRTREPVIRQIFIGRGPDI
                     MVTDALERKLYVIRRSAANAVQALGLKHSKEFYQPSMSARTIVYKGLLLANQVGEYYT
                     DLKDPRCISAMALVHQRFSTNTFPTWQLAHPFRMIAHNGEINTLRGNYNWMRAREKGT
                     SSPLLGADLEKLWPLIYPGQSDSASFDNALELLVMGGYSLAHAMMMLIPEAWESHTLM
                     DEKRGAFYKYHAAMIEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLVTDDDLVVMAS
                     EAGVLPIPEERIVKKWRLQPGKMFLIDLDQGRIIDDLELKDTLARTKPYQDWLSRINI
                     RLDDLEVPNNLPETDCSASLLDRQQAFGYSQEDLKFILEPMAASGEEATGSMGNDSPL
                     AVLSDRSKPLFNYFRQLFAQVTNPPIDPIREQLVMSLVSFIGPKPNLLGINEINPPYR
                     LEVAQPVLDFDNMAKLRRIATYTGNKFHSAELDICYPLAWGNEGVEARLASLCAEAED
                     HVHKGSSILIVSDRRIDAEHVAIPALLATSAVHHHLVTKGLRTRVGLVVETGAARETH
                     HFALLAGYGAEAVHPYLAFETLRQLAGGDAEKGEKAIKHFIKGVGKGLLKVMSKMGIS
                     TYMSYTGAQIFEAIGLQKKMVDKYFSGTSTQVEGIGVFEVMEEAIRLHKKAFGDDPVL
                     ATMLDAGGEYAYRVRGEEHMWTPDAIAKLQHATRTGKYETYKEYAALINDQTRRHMTL
                     RGLFEFKAAGPALSLDEVEPAKEIVKRFTTGAMSLGSISTEA"
BASE COUNT          619 a          893 c          955 g          616 t
ORIGIN      
        1 cgagaataag ggtcaccgct atccctgtcg ccgaactgct gtttcactgt tccggtccca
       61 aacaggccgg ttcgctcggt caagctttcg cagggtcggt tgtggcgggt caggcatatt
      121 aattgcatat agtgcaaaaa ggctcgttga ggctgtgctg gcacagagaa atggccgcca
      181 ctgcttcatt gcggtgccaa agcgtcaact gcgcgcactc ccttggtgcg gcgcaacatc
      241 ttgaatggca ggagtattca gggtatagtc gccagtcccc gtgggtgggt cggtggtcgg
      301 tttgcgccgg ttttgcggcg gttttgtggc agttatgcgc ccaaccgatt gccggggtac
      361 cgttttttgt tttgtgagga ttcgagcgtg atgaattcgg ccatacctga agggcagggg
      421 ctttacgacc cggccaacga gcacgatgcc tgtggcgtcg gttttgtagc gcacatccgg
      481 ggcaagaaga gtcactcgat catcgagcag ggcctgttga tcctgaagaa tctcgatcac
      541 cgcggggcgg ttggtgctga tccgctgatg ggcgacgggg ccgggatcct gattcagatt
      601 ccggatgcct accttcgtga ggagatggcc aagcaggggg tgcaattgcc gcaacccgga
      661 gactatggcg ttggaatggt cttcctgccg aaggagtcag cttctcgcct tgcctgtcag
      721 gaagaaatcg aacgcgcagt gcgctccgag catcaggtcg ttctgggttg gcgcgatgtg
      781 ccgatcgacc acgctttgcc gatgtcgccc accgtgcgca ccagggagcc ggtcatccgc
      841 cagatcttca tcggtcgtgg tccggacatc atggtgaccg acgctctcga gcgcaagctc
      901 tacgtgatcc gtcgtagcgc agccaacgcc gtgcaggcgc ttggactgaa gcacagcaag
      961 gaattttacc aaccgtcgat gtcggcacgg acgatcgtct acaaagggtt gctgctcgcc
     1021 aatcaggtcg gcgagtacta caccgacctc aaggatccgc gttgcatctc ggcaatggcc
     1081 ctggtgcacc agcgcttttc gaccaatacc tttccgacct ggcaactggc gcacccgttc
     1141 cgcatgatcg cccacaacgg tgaaatcaac accctgcgcg gcaactacaa ctggatgcgg
     1201 gcgcgcgaga aaggcacctc gtcaccgctt ctcggcgccg atcttgaaaa actttggccg
     1261 ctgatctacc cgggtcaatc cgattccgct tccttcgaca acgcgctcga actgctggtg
     1321 atgggcggct attcacttgc gcacgcaatg atgatgctga tcccggaagc ctgggagtcg
     1381 cataccttga tggacgagaa gcgtggcgct ttctacaagt accatgcggc gatgatcgag
     1441 ccttgggatg gtccggcggc cgtggcgttt accgacgggc gccagatcgg cgcgacgctc
     1501 gaccgcaacg gcttgcgacc ggcacggtat ctggttaccg acgacgatct ggtggtgatg
     1561 gcctcggaag ccggggtgct accgattccc gaagaacgga tcgtcaagaa gtggcggctg
     1621 caaccgggca agatgtttct gatcgacctc gaccaggggc ggatcatcga cgatctggaa
     1681 ctcaaggaca cgctggccag gaccaagcct taccaggact ggctgagccg catcaatatc
     1741 cggctcgatg acctggaggt cccgaataac ctgccggaga cagactgttc cgcgtcgctg
     1801 ctcgaccgtc agcaggcgtt cggctacagt caggaggatc tcaagttcat actggagccg
     1861 atggccgcct cgggcgagga ggcgaccggt tcaatgggca acgattcgcc actggcggtc
     1921 ttgtcggacc gaagcaagcc gctgttcaat tattttcgcc agctctttgc gcaggtgacc
     1981 aatccgccga ttgacccgat ccgcgagcaa ctggtgatgt cgctggtttc gttcatcggc
     2041 cccaaaccca atctgctcgg gatcaacgaa atcaatccac cctatcgtct cgaagtcgcc
     2101 cagccggtac tcgatttcga caacatggcg aagttgcggc gtattgcgac ctacaccggc
     2161 aacaagttcc actccgccga actcgacatc tgttacccgc tggcgtgggg caatgaaggc
     2221 gttgaagccc gtctggcttc gctgtgtgcg gaagccgagg accacgtcca caaggggtcg
     2281 agcatcctga tcgtatcgga tcgacggatc gatgccgagc atgtcgcgat tccggccttg
     2341 ctggccacct cggcggtgca tcaccatctg gtcaccaagg gtttgcgcac gcgtgtcggg
     2401 ctggttgtcg aaaccggcgc agcgcgcgaa acgcatcatt tcgccttgct tgccggttac
     2461 ggcgccgaag ccgtgcaccc gtacctggcg ttcgaaacac tccggcagtt ggctggcggg
     2521 gacgccgaga agggcgagaa ggcgatcaag cacttcatca agggcgtcgg caaaggcctg
     2581 ctcaaggtga tgtcgaagat gggcatttcg acttacatgt cgtacaccgg agcacagatc
     2641 ttcgaggcga tcggtcttca gaagaagatg gtcgacaagt acttcagcgg aacttcgacg
     2701 caggtggaag gcatcggtgt cttcgaggtg atggaagaag cgattcgcct gcacaagaag
     2761 gctttcggcg acgaccccgt gttggccacc atgctcgacg ccggcggcga gtacgcttac
     2821 cgcgtgcgtg gcgaagagca catgtggacg cccgatgcga ttgccaaact gcagcacgcg
     2881 acacgcacag gaaagtacga gacctacaag gaatatgccg cgctgatcaa cgatcagacg
     2941 cggcggcaca tgacgctgcg cgggttgttc gagttcaagg ctgccgggcc ggcgctgtcg
     3001 cttgacgaag tcgagccggc caaggaaatc gtcaaacgct tcaccaccgg cgcgatgtcg
     3061 ctcgggtcga tctccaccga ggc
//
LOCUS       DAOSIT010000261         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27579_length_3083_cov_6.414135, whole genome shotgun sequence.
ACCESSION   DAOSIT010000261 DAOSIT010000000
VERSION     DAOSIT010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27579_length_3083_cov_6.414135"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3008)
                     /locus_tag="PLL14_07110"
     CDS             complement(<1..3008)
                     /locus_tag="PLL14_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOG03733.1"
                     /translation="MTTKSERLTVLSDAEQEALYGLPDFDDAQRLEYLALTETELALA
                     SSRPGLHAQVYCILQIGYFKAKHAFFRFDWSEVEHDCAFVLSRYFHGESFEHKPISKH
                     EHYTQREWIADLFGYRPWAAEFLAQLAQQAAQTVRRDVMPGFIAAELIVWLNEHKIIR
                     PGYTTLQELVSEALSAERRRLAGLLSEVLDESAKAALGRLLVRDDTLSQLAALKQDAK
                     DFGWRQMAREREKRATLEPLHRIAKALLPKLGVSQQNLLYYASLANFYTVHDLRNLKA
                     DQTYLYLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFVAEQVRRQQDTPQ
                     VGRLLSLYIDDSVPDPTPFGDVRQRAYKIMPRDTLQTTAQRMSVKPVSKLALHWQAVD
                     GLAERIRRHLRPLYVALDLAGTDPGSPWLVALAWAKDVFAKQQRLSQRPLAECPAATL
                     PKRLRPYLLTFDADGKPTGLHADRYEFWLYRQVRKRFQSGELYLDDSLQHRHFSDELV
                     SLDEKAAVLAQIDIPFLRQPLDAQLDALATELRAQWLAFNRELKQGKLTHLEYDKDTQ
                     KLTWRKPKGENQKAREKAFYEQLPFCDVADVFRFVNGQCQFLSALTPLQPRYAKKVAD
                     ADSLMAVIIAQAMNHGNQVMARTSDIPYHVLESAYQQYLRHATLHAANDCISNAIAAL
                     PIFPYYSFDLDALYGAVDGQKFGVERPTVKARHSRKYFGRGKGVVAYTLLCNHVPLNG
                     YLIGAHDYEAHHVFDIWYRNTSDIVPTAITGDMHSVNKANFAILHWFGLRFEPRFTDL
                     GDQLKELYSADDPALYDQCLIRPAGRIDRDLIVSEKPNLDQIVATLGLKEMTQGTLIR
                     KLCTYTAPNPTRRAVFEFDKLIRSIYTLRYLRDPQLERNVHRSQNRIESYHQLRSTIA
                     QVGGKKELTGRTDIEIEISNQCARLIANAVIFYNSAILSRLLMKYEASGNAKAHALLT
                     QISPAAWRHILLNGHYTFQSDGKMIDLDALV"
BASE COUNT          517 a          925 c         1033 g          608 t
ORIGIN      
        1 acgagcgcat ccaggtcgat catcttgccg tcgctctgga aggtgtaatg cccgttcagc
       61 aggatgtgcc gccaggccgc cggcgatatc tgggtcagga gagcgtgcgc cttggcgttg
      121 ccgctcgcct cgtacttcat cagcagccgc gagaggatgg ccgagttgta gaagatgacc
      181 gcgttggcga tcagcctggc gcactggttg ctgatctcaa tttcgatgtc ggtgcgcccg
      241 gtcaattcct tcttgccgcc gacctgggcg atggttgagc gtagctggtg ataggactcg
      301 atgcggttct gtgagcggtg aacgttgcgc tccagttgcg gatcgcgcag gtagcgcagc
      361 gtgtagatgc tgcggatgag cttgtcgaac tcgaacaccg cgcgccgcgt ggggttcggc
      421 gcggtgtagg tgcatagctt gcggatcagc gtgccctgcg tcatctcctt cagtccgagc
      481 gtggcgacaa tctggtcgag gttcggcttc tcgctgacta tgagatcgcg gtcgattctc
      541 ccggccggcc ggatcaggca ctgatcgtac agcgccggat cgtcggcact gtagagttcc
      601 ttcaactgat cgccaaggtc ggtgaagcgc ggctcgaaac gcaggccgaa ccagtgcagg
      661 atagcgaagt tggccttgtt gacgctgtgc atgtcgccgg tgatcgcggt cggcacgatg
      721 tccgacgtgt tgcgatacca gatgtcgaac acgtgatggg cctcgtaatc gtgcgcgccg
      781 atcaggtagc cgttgagcgg cacgtggttg cacagcagcg tgtaggcgac cacgcccttg
      841 ccgcgcccaa agtatttgcg cgagtggcgc gctttcacgg tcggccgctc gacgccgaat
      901 ttctgaccat cgacggcacc gtacagtgca tcgaggtcga acgagtagta cgggaagatc
      961 ggcagcgcgg cgatggcgtt gctgatgcag tcgttggccg cgtgcagcgt tgcgtggcgc
     1021 aggtactgtt ggtaggcgct ctccagcacg tggtacggga tgtcgctggt gcgtgccatg
     1081 acctggttgc cgtggttcat cgcctgcgcg atgatgaccg ccatcaggct gtcggcgtcg
     1141 gcgaccttct tcgcatagcg cggctgcaaa ggcgtcagcg ccgacaggaa ctggcactgg
     1201 ccgttgacga agcggaacac gtcggccacg tcgcagaacg gcagttgctc gtagaacgcc
     1261 ttctcgcgcg ccttctggtt ctcgcccttg ggcttgcgcc atgtcagctt ctgcgtgtcc
     1321 ttgtcgtatt ctaggtgcgt cagcttgccc tgcttcagct cgcggttgaa ggccagccac
     1381 tgagcgcgca gctcggtcgc gagcgcatcg agctgggcat cgagtggctg ccgcaggaac
     1441 gggatgtcga tctgcgccag cacggcggcc ttctcatcca gcgaaaccag ctcgtcggaa
     1501 aaatgccggt gctgcaagct gtcgtcgagg tagagttcac ccgactggaa gcgcttcctg
     1561 acctggcggt acagccagaa ctcgtagcgg tcggcatgca ggcccgtcgg cttgccatcg
     1621 gcatcgaagg tcagcaggta cggtcgcaag cgtttcggca gcgtggccgc tggacattcg
     1681 gcgagcggcc gttgcgatag gcgctgctgt ttggcgaaca cgtccttggc ccaggccagc
     1741 gccacgagcc acgggctgcc cggatcagtg ccagcgaggt cgagcgcgac atacagcggc
     1801 cgaagatggc ggcggatgcg ctcagccagg ccgtccaccg cctgccagtg caaagccagc
     1861 ttgctcaccg gcttcacgct catgcgctgc gcggtggttt gcagcgtatc gcggggcatg
     1921 attttgtagg cgcgctggcg cacatcgccg aacggcgtgg gatcgggcac gctgtcgtcg
     1981 atgtaaagcg acagcaggcg gccgacctgc ggtgtgtctt gctgacggcg cacctgctcg
     2041 gcgacaaagg attgctttgc gcccgcactg ctttcgtcct ccaactgctt catgtggtag
     2101 gccatcgcat cgaccaggtt gtcggaaagc tgccggtagc gcacccaggc atagcaaagc
     2161 aggtagaggt aggtctgatc ggccttcagg ttgcgtagat cgtggacggt gtagaagttc
     2221 gccaggctgg cgtagtacag cagattctgc tgcgagacgc cgagcttggg cagcagcgcc
     2281 ttggcgatcc ggtgcagcgg ctccagcgtg gcgcgctttt cgcgttcgcg ggccatctga
     2341 cgccagccaa agtccttggc gtcctgcttg agcgccgcca attgcgacag ggtgtcgtca
     2401 cgcactagaa gccgacccag cgcggccttg gccgattcgt ccaacacttc cgacagcagg
     2461 ccagccagcc gccgacgctc ggcggacagg gcttcgctca ccagctcttg cagggtggta
     2521 tagccgggcc ggatgatctt gtgctcgttt agccagacga tcagctcggc ggcgatgaac
     2581 cccggcatca cgtcgcgccg cacggtctgc gcggcctgct gcgcgagctg cgccaggaac
     2641 tcggccgccc acggccggta gccgaacaga tcggcaatcc actcgcgctg ggtgtagtgc
     2701 tcgtgcttgg agattggctt gtgctcgaag gactcgccgt ggaagtagcg gctcagcacg
     2761 aaggcgcaat cgtgctcgac ctcactccag tcgaagcgga agaaggcatg cttggccttg
     2821 aagtaaccga tctgcaagat gcaatagacc tgggcatgga gaccaggccg gctgctggcg
     2881 agcgccagtt cggtttcagt caacgccaag tattccagcc gctgggcgtc gtcgaagtcc
     2941 ggcaggccgt acagggcttc ctgctcggcg tccgacagga cggtcaatcg ttcgctcttg
     3001 gtcgtcatcg cgatgacgct ccacgagagc ccgtccaacc aacccgtgcc agggtctcga
     3061 tcagcgtggt tcgcttgacg ccg
//
LOCUS       DAOSIT010000262         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27597_length_3081_cov_8.087905, whole genome shotgun sequence.
ACCESSION   DAOSIT010000262 DAOSIT010000000
VERSION     DAOSIT010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27597_length_3081_cov_8.087905"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLL14_07115"
     CDS             complement(<1..488)
                     /locus_tag="PLL14_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG03734.1"
                     /translation="MHAFEVSDPFAIRALTPTRPALTISGELQRMPCQALPADAVYGI
                     LEVVDLALCKNPTTREAWSYARVQAAQVGLAQSDFLPTVDGQVAASRIRADSRSTTQR
                     NASLTLSWLLFDFGARSANLEVATQLFNAAVSTLDSTVQSVFLGALQAYYNVQAARAA
                     VTA"
     gene            867..1202
                     /locus_tag="PLL14_07120"
     CDS             867..1202
                     /locus_tag="PLL14_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HOG03735.1"
                     /translation="MPWMEAVGLLVMLAGGWFWLDSFKAREAGMAAARAACAAENLLL
                     LDDTVALGALHPARDDDGRLRLRRVYHFEYSDTGDNRRHGRVTLLGSELLMVHLPQTR
                     APRLCSPSA"
     gene            complement(1199..2095)
                     /locus_tag="PLL14_07125"
     CDS             complement(1199..2095)
                     /locus_tag="PLL14_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOG03736.1"
                     /translation="MNTAQSILLLAVPALLAGTPVLAKEGPDQYPNGSENFMAGALPP
                     PGNYFINYLGYYQGDYRDNDGHKVPGLNVSATFDALRFIHVTNQKVFGGDWAVHAIVP
                     LVSQRMHTPFPGIGNGSVFGLGDIVIDPLIIGWHFPPDWHLTVGIDIYLPTGKYSKSN
                     PTDSIGANYYSFEPIFAFTYLNQSGFEASAKLMYNLKTKNLDTDYQSGNEFHMDYLIG
                     QHFGPWAVGLAGYYLRQTTDDQVNGQKVGPDGRRGQVFAYGPAIKYDYKGMSFVGSWN
                     HESSVDNRFQGNKFFFKWVTAF"
     gene            complement(2092..>3081)
                     /locus_tag="PLL14_07130"
     CDS             complement(2092..>3081)
                     /locus_tag="PLL14_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HOG03737.1"
                     /translation="LVMPSRNMDAADADFAVCCAVPVDAPGLTMVARPAGRPGEKLEH
                     GAALFSRRYGQSTAVLIFERVFVPWERVFFAGEWEHSGAVTYHYATHHRHSCIAARAG
                     FGDLLIGAGALMCEANGFDPGRKASLREPMVELIKITEGFFACGVAASVYGTRDEHSG
                     SFMPEPVFANIGKLLLSTQIYDMHRLAHEVSGGLIVALPGPDEDHNPATSATLAEVLR
                     ANPMVPYDKRIEVARFIEDLSASYQGGWYSLISLHGGGSPAALKQEIWRHYPLGNKVE
                     LVERLLDRGVLHDDARPITRNRQPGRCCDAGCSPPGQAIMLPLPYTNKGEENQ"
BASE COUNT          547 a          899 c         1047 g          588 t
ORIGIN      
        1 gcggtgaccg cggcgcgcgc ggcctgaacg ttgtaatagg cctgcagggc accgagaaaa
       61 acgctctgca cggtcgagtc gagcgtcgaa acggcggcgt tgaataactg cgttgccact
      121 tcgagatttg ccgaacgggc accgaagtcg aacagcaacc acgagagcgt cagcgaggca
      181 ttgcgctggg tggtgctccg tgaatcggcc ctgatccggc tggcggccac ctgaccatcg
      241 accgtcggca ggaagtcgga ctgcgccagg ccgacctggg cggcttgcac gcgcgcgtac
      301 gaccacgcct cacgggtcgt cgggttcttg cacaatgcca ggtcgacgac ttccagtatc
      361 ccatagacag cgtcggcggg cagtgcctgg caaggcatgc gctgaagctc gccgctgatc
      421 gttagcgcgg gacgtgttgg ggtaagcgcg cggatggcga acgggtcgct gacttcgaag
      481 gcatgcaacg gggtcgccaa tgctgcacac aaggtggcga gggcgaggcg gggtaatgtg
      541 gcgccatggg cgagcgcagt cggtcgggat gtgatgtagg ccatttttcg ctagtatagc
      601 gaggcttgcc gcgatgtctt gcgacactga cgccttggtg cagcgtccgg caggtttttc
      661 cattagcaaa tgttaccacc cgcaacggcg accggccgcg gcgatacgca cttcaggagg
      721 gcagcggcga tgacgacgag cgcctgcgag gcgccttgcc ggtgggcatg cgagggaagt
      781 caggcggata ctcgggcgga tgggcaagcc tccgtaggcc aaggtcgtat cgccgccgcg
      841 cggccgtctt ccacgggagc cgggtgatgc cgtggatgga agcggttggc ctcttggtca
      901 tgctggccgg agggtggttc tggcttgaca gcttcaaggc gcgcgaggcc ggcatggctg
      961 ccgcgcgtgc cgcctgtgca gcagagaacc tgctgctgct tgacgacacc gtggcgctgg
     1021 gggcattgca tccagcgcgt gacgacgatg gccggctgcg cctgcggcgc gtctatcatt
     1081 ttgaatatag tgataccggg gataatcgcc gtcatggtcg ggtgaccctg ctcggcagcg
     1141 agttgctgat ggtgcatctg ccgcagacgc gcgcgccgcg gctgtgctcg ccttccgcct
     1201 agaacgcagt gacccacttg aaaaagaact tgttgccctg gaagcggttg tcgacgctgc
     1261 tctcgtggtt ccaggatccg acgaaactca tccccttgta gtcgtacttg attgccggac
     1321 cgtaggcgaa gacctgaccg cgccggccat ccgggccgac tttctggccg ttcacctgat
     1381 cgtcggtggt ctgccgcagg tagtagccgg ccagcccgac ggcccacggc ccgaagtgct
     1441 ggccgatcag gtagtccatg tggaactcat tgcccgactg atagtcggtg tcgaggttct
     1501 tggtcttgag gttgtacatc agcttggcgg atgcttcgaa accgctttgg ttcaggtagg
     1561 tgaaagcgaa gatcggctcg aaactgtagt agttggctcc gatactgtcg gtagggttgc
     1621 tcttgctgta cttgccggtg ggcaggtaga tgtcgatacc gacggtcagg tgccagtccg
     1681 gcgggaaatg ccagccgatg atcagcgggt caatgacgat gtcaccaaga ccgaagacgc
     1741 tgccgttgcc gatacctgga aacggagtgt gcattctctg ggagacgagc ggtacgatcg
     1801 catgcaccgc ccagtcacca ccaaagacct tctgattggt gacgtggatg aagcgcagag
     1861 cgtcgaaagt ggcactgacg ttcagtcctg gtaccttgtg gccgtcgttg tcgcgatagt
     1921 caccctggta atagccgagg tagttgatga agtagttgcc gggcggcggc agcgcgccgg
     1981 ccatgaagtt ctcggatccg ttcggatatt ggtcaggccc ctccttggcg aggaccgggg
     2041 tgccggcgag cagggccggc accgccagaa gcagaatgct ttgcgcggtg ttcattggtt
     2101 ttcttctccc ttgttggtgt acggcagcgg cagcatgata gcttgtcccg gtggtgagca
     2161 gccagcatca caacagcggc ctggttgtcg attgcgggtg atcgggcgcg cgtcatcgtg
     2221 cagtacgccc cggtcaagca gccgttcgac cagttcgacc ttgttgccca gcgggtagtg
     2281 gcgccagatc tcctgcttca atgccgccgg cgaaccgccg ccatgcagac tgatcaacga
     2341 ataccagccc ccctggtacg aagcgctcag gtcttcgatg aaacgggcga cctcgatccg
     2401 cttgtcgtag ggcaccatcg ggttggcgcg caggacctcg gcgagggtcg ccgaggtcgc
     2461 cgggttgtgg tcttcatcgg gaccgggtag cgccacgatc aacccccccg acacttcgtg
     2521 tgccagacga tgcatgtcgt agatctgggt ggagagcaga agtttgccga tattggcaaa
     2581 caccggttcg ggcatgaagc tgccgctgtg ctcgtccctg gtgccgtata cgctggccgc
     2641 gacaccacag gcgaagaagc cttcggtgat cttgatcagt tcgaccatgg gttcgcgcag
     2701 gctggccttg cggcccggat cgaagccgtt ggcctcgcac atcagggcac cggcaccgat
     2761 cagcaggtcg ccgaagccgg ctctggcggc gatgcagcta tggcgatgat gcgtggcgta
     2821 gtggtaggtc actgctccgg aatgctccca ctcgccggca aagaacaccc gttcccaagg
     2881 cacgaaaacg cgctcgaaga tcagcaccgc ggtcgattgt ccatagcgcc gcgagaacag
     2941 ggcggcgccg tgttcgagct tttctccggg gcgccctgcc gggcgggcaa ccatcgtcaa
     3001 gcccggcgcg tcgaccggca ccgcgcagca cacggcgaaa tcggcgtcgg ccgcgtccat
     3061 gttgcggctc ggcatgacca g
//
LOCUS       DAOSIT010000263         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27640_length_3077_cov_7.316678, whole genome shotgun sequence.
ACCESSION   DAOSIT010000263 DAOSIT010000000
VERSION     DAOSIT010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27640_length_3077_cov_7.316678"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLL14_07135"
     CDS             complement(<1..632)
                     /locus_tag="PLL14_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOG03738.1"
                     /translation="MPLNRKSIELLAPAKNAEFGIEAIIHGADAVYVGGPAFGARTKA
                     DNSVADIGRLASFAHRYNARVLVAMNTLLKDEELEQARCLIWQLYDAGADALIIQDMG
                     LLELDLPPIQLHASTQTNIRSLEKARFLEDVGFSQIVLARELALDQIRMIANETRIVL
                     EFFIHGALCVSYSGLCNLSYAQTGRSANRGECSQACRLPYSLASQNGQLVA"
     gene            818..1102
                     /locus_tag="PLL14_07140"
     CDS             818..1102
                     /locus_tag="PLL14_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03739.1"
                     /translation="MGSLSTESFDQFLDSLKQAGVEISNECELRERLAEAQRWRFAFA
                     TLAANGRPLGIRFQDRSSGANEADIHRTLARFQFPEVLQTTFAASLRAEH"
     gene            complement(1348..>1582)
                     /locus_tag="PLL14_07145"
     CDS             complement(1348..>1582)
                     /locus_tag="PLL14_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03740.1"
                     /translation="LGLPLWLSVRRSVGLAVSRSDGDFDLDDLIPLLIGAISLWYGEK
                     FTKPPTRVLRNWIVHADIMTHTAAAVQQACTCR"
     assembly_gap    1583..1682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1683..1789)
                     /locus_tag="PLL14_07150"
     CDS             complement(<1683..1789)
                     /locus_tag="PLL14_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG03741.1"
                     /translation="MRQIFLDTETTGLEHKLGHRIIEIAGVEMFNRRLT"
     gene            complement(1848..2315)
                     /gene="rnhA"
                     /locus_tag="PLL14_07155"
     CDS             complement(1848..2315)
                     /gene="rnhA"
                     /locus_tag="PLL14_07155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOG03742.1"
                     /translation="MSHENIINIYADGGCRGNPGPGGWGVLLQAGGQEKELWGGEKAT
                     TNNRMEMTAAIRALEALRKPATVHLHTDSQYLQKGISEWIHNWKRNGWRTSDKKPVKN
                     ADLWQRLDELAGQHQVKWCWVKGHAGHVGNERADALANRGMDELQRAPTSITS"
     gene            complement(2305..3030)
                     /locus_tag="PLL14_07160"
     CDS             complement(2305..3030)
                     /locus_tag="PLL14_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG03743.1"
                     /translation="MSIAGLDDWLQSPQGNYVMAWEQARIDAIVADLFGYNAIQLGLP
                     QRDLLAQNRIPLRQVVGDSATANVVCDLRQLPFAAHSIDLVVMAHVLEFHAHQHQILR
                     EVERVLIPEGEVLITGFNPLSLWGLRRRLPNCPGGFPWNGHYLSVPRLKDWLQLLGFE
                     VDRGSFGCYSPPCHHQRWLKRWHFVEATGNRWWNPAGGVYLLRAIKRVHGMRLILPNW
                     KQQASPRKALSVVSKKEIEQHES"
BASE COUNT          581 a          839 c          912 g          645 t
ORIGIN      
        1 gcgacgagtt ggccgttctg gctggcgagc gaatacggca gtcggcaggc ctgcgagcac
       61 tcgccacggt tggcgctacg cccggtctgg gcatagctga ggttgcacag gccactgtaa
      121 ctgacgcaaa gcgcgccatg gatgaagaat tccagcacga tcctggtttc gttggcgatc
      181 atacgaatct gatcgagcgc cagttcgcgc gccagaacga tctgggagaa acccacatcc
      241 tcgaggaaac gtgctttctc aagcgaacgg atgttggttt gcgtgctggc gtgcaactgg
      301 atcggcggca ggtcgagctc gagcagcccc atgtcctgga tgatcagggc gtctgcgccg
      361 gcatcgtaga gctgccagat caggcagcgc gcttgctcaa gttcctcgtc tttgagcagg
      421 gtattcatcg ccaccagtac ccgtgcgttg tagcggtggg cgaagctggc gaggcgtccg
      481 atatcggcca cggagttgtc tgccttggtc cgagcgccga acgccggacc gccgacatag
      541 acggcgtcag cgccatggat gatcgcttcg atcccgaact cggcattctt ggcgggagcc
      601 agcagttcga tactttttcg gtttagaggc atgtggggcc gcgcgcaagc aatgcaagca
      661 ttgtataccg tacgcacgtc aaagctgacg gtcatggcga ctgtactggg ccgtgtggga
      721 agggcagctt tcttgaccct ggctgtcaac cgctgcgcaa agcgagcgtt actcggagca
      781 tgggtgagac actaaaatac ttcgacagga gaacaaaatg ggtagcctaa gcaccgagtc
      841 ttttgaccag tttctggatt cactcaagca ggctggtgta gaaatcagca atgagtgcga
      901 attgcgggag cggttggcag aagcccagcg ctggcgtttt gcttttgcca ccttggcggc
      961 caatggccga ccgttgggaa ttcgctttca ggacaggagc agtggggcta acgaggcaga
     1021 tattcaccgg acccttgcgc gcttccagtt tcctgaagtc ctgcagacga cgtttgctgc
     1081 cagcttgcgg gctgagcatt gattgcaagc tgatctcccc gcgggaagat cagtctcatc
     1141 gagtccgccg atccttgggt cactccgggg tgtcggggaa ctaaaaggcc cgcttcgaag
     1201 cgggcctttt tcagtgaacc tgacgcccga ctggctgtaa ctcgggtggc cgacaatata
     1261 cctcggctct ccgcgcatca gtctcaaagc ggcttggctt gtgaggggtg taggctccct
     1321 gcgaggatgc ttctgaacgc tgcgaggtca tcgacacgtg caagcttgtt gaaccgctgc
     1381 cgctgtgtga gtcataatgt cggcatgaac gatccaattc ctcaagaccc gcgtcggcgg
     1441 cttcgtgaac ttctcgccat accagagcga gatcgctccg atgagcagtg ggatgagatc
     1501 atcgagatcg aaatcaccat cgctccggga aaccgcgagt ccgaccgacc tgcggacaga
     1561 cagccagagc ggcaggccga gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnttggtcag acgccgattg aacatctcga caccagcgat ctcgatgatg cggtgtccga
     1741 gcttgtgctc caggccggtg gtttcagtat cgagaaagat ctggcgcatc agtaggttcc
     1801 ctggtagtga aggtcgcttc agcgaatcgt gatcggtgct gcggctgtca ggacgtaatg
     1861 cttgtcggcg cccgttgcag ttcgtccatg ccgcgattgg ccagggcgtc ggcacgctcg
     1921 ttgccgacgt gacccgcatg tcctttgacc cagcaccact tgacctggtg ttgcccggca
     1981 agttcatcga gtcgctgcca aaggtcggcg ttctttaccg gcttcttgtc cgaggttcgc
     2041 cagccattgc gcttccagtt gtggatccac tcgctgatgc ctttttgcag gtattgcgaa
     2101 tcggtatgca aatgtacggt cgcaggcttc ctgagcgctt ccagggcgcg tatggcggcg
     2161 gtcatttcca tgcggttgtt ggtggtggct ttttcgccgc cccatagttc cttttcctga
     2221 ccaccagcct gcaggagaac tccccaacca ccgggcccag ggttgccacg acagcctccg
     2281 tcggcgtaga tgttgatgat gttttcatga ctcatgctgt tcaatctcct tcttgctgac
     2341 taccgacaag gcctttcgcg gtgaagcttg ctgcttccag ttcggaagga tcaagcgcat
     2401 gccatgcacg cgcttgatcg cacgcagcag gtagacacct cctgcagggt tccaccaacg
     2461 attgccggtg gcctccacga aatgccagcg tttgagccag cgttgatgat ggcagggtgg
     2521 cgaataacag ccgaagctcc cgcggtcgac ctcaaaaccg agaagttgca gccagtcctt
     2581 gaggcgcggc accgagagat agtggccgtt ccacgggaag ccgccggggc agttgggcag
     2641 ccggcgccgg agcccccaaa gtgagagcgg attgaagccg gtaatcagca cttcgccttc
     2701 cggaatcaaa actcgttcaa cctcgcgcaa tatctggtgc tggtgggcat ggaactccag
     2761 cacgtgcgcc atgaccacca ggtcgatgct gtgcgctgca aatgggagtt gacgcagatc
     2821 gcagacgacg ttggcggttg ctgaatcgcc aaccacctgt cgcaagggga tgcggttctg
     2881 ggccagcagg tcgcgttgcg gcaggcctag ctggatcgcg ttgtagccga aaaggtcggc
     2941 aacgatggcg tcgatccgtg cctgctccca ggccatgacg tagtttcctt gcgggctttg
     3001 cagccagtcg tcgagtccag cgattgacat tttccgcaat tcttccaaaa ttcaatgatg
     3061 ttcgacgtca tccgtat
//
LOCUS       DAOSIT010000264         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27691_length_3072_cov_9.202519, whole genome shotgun sequence.
ACCESSION   DAOSIT010000264 DAOSIT010000000
VERSION     DAOSIT010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27691_length_3072_cov_9.202519"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="cas1"
                     /locus_tag="PLL14_07165"
     CDS             complement(<1..1006)
                     /gene="cas1"
                     /locus_tag="PLL14_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOG03744.1"
                     /translation="MTSLYVDRRGVTLKADGEALVFYENGERIGTVPLAPLSRVFMRG
                     DVTLSSALLGKLGERGIGVVVLSGRKAVPTMLLGRPHNDAARRVAQYRLSLDESFCLR
                     FARAIVEAKLRAHAGFLAERRDTEPQSRYLLTLCLQRLATSIAAVDQQGSIASLRGLE
                     GAGAAAYFEGFADLLPPRLKFSGRNRRPPRDPVNALLSLGYTLLHAEAVLALYGAGLD
                     PFIGFYHALDYGRESLACDLVEPLRVEVDRHALMLFRSEKLRPEDFSITESGCLLGKA
                     GRARFYSEWEQLAARLRKLLAESVADVAGAIMQAGGVEAPRSPASDDAAAEAPGTEAD
                     D"
     gene            complement(1023..1310)
                     /gene="cas2"
                     /locus_tag="PLL14_07170"
     CDS             complement(1023..1310)
                     /gene="cas2"
                     /locus_tag="PLL14_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOG03745.1"
                     /translation="MSDAARTLFLVCYDVCKTSMRTRVRKFLMAYKVGGQKSFFECWL
                     TAAELREVRLTLAELIDPAEDRVHIFQLDPRMRRDLLGRATEPVTDVFLIV"
     assembly_gap    1312..1411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1457..>3072
                     /locus_tag="PLL14_07175"
     CDS             1457..>3072
                     /locus_tag="PLL14_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG03746.1"
                     /translation="MHVVAQTHFGTTAALPVGDNRPSDELQKALAQGSRQGTYASAST
                     TADGVSRIHAYHRSEKYGFTVLVGIPQSLITSIWLKQSAIVALLLVAILIGLLLGTQL
                     VSRRLADQLGRSVSEAREAERELFRTLIRSIPNLIWLKNAEGVYLACNREFEKFFGKS
                     EAEIVGKTDYDFLSGELADFFRGHDRAAAAAGKPTVNEEWITYADDGHRALLRTTKTP
                     MIAADGRLIGVLGIAHDITEMRRQEEALRESRETLNRAQAVAHVGSWMLDIEANRLEW
                     SDECYRLFGLPPGSPMTLERFFACIHPDDLERVAAAWHATLGGAEYDIEHRILAAGET
                     RWVRERAIIERDADGKARRGLGTVQDITLRRQTEAALQEERRVRDTLLESIPGVFYAI
                     DARGMFTFWNHHFEQVTGRSAEELRHFNALDLFEGEDREHVAERIEQVFLEGQSDIEA
                     SLVTKDGQHLPYYFTGRRIEMSGQPILVGAGMDIGPLKAADEAMRRINEELEARVRQN
                     TADLQASYAKLRDTEFAMNQAGIGIAWVNT"
BASE COUNT          533 a          965 c          991 g          483 t
ORIGIN      
        1 catcgtccgc ctccgtgcct ggtgcctcgg ccgccgcgtc gtcgctcgcc ggcgagcggg
       61 gggcctcgac gccgccggcc tgcatgatcg cgccggccac gtcggcgacg ctctcggcca
      121 acagcttgcg caggcgcgcc gcgagttgct cccactcgct gtagaagcgc gcgcgcccgg
      181 ccttgccgag caggcaaccg ctctcggtga tcgagaaatc ctcggggcgc agtttctccg
      241 atcggaacag catcagcgcg tgccggtcga cttcgacgcg cagcggctcg acgaggtcgc
      301 aggccagcga ttcgcggcca tagtcgagcg cgtggtagaa gccgatgaag gggtcgagcc
      361 cggcgccgta cagcgccagc accgcttcgg cgtgcagcag cgtgtagccg agcgagagca
      421 gggcgttgac cgggtcgcgc ggcggccggc ggttgcggcc gctgaatttc agacgcggcg
      481 gcagcaagtc ggcgaagcct tcgaagtagg cggcggcgcc ggcgccttcg aggccgcgca
      541 gcgaggcgat cgagccttgc tgatcgaccg ccgcgatgct cgtcgccaga cgctgcaggc
      601 agagggtgag caggtagcgc gactgcggct cggtgtcgcg gcgctcggcg agaaaaccgg
      661 cgtgcgcgcg cagcttggct tcgacgatgg cgcgcgcaaa gcgcaggcag aaggattcgt
      721 cgagcgacag ccggtattgc gccacccgcc gcgccgcgtc gttgtgcggc cgcccgagga
      781 gcatcgtcgg taccgccttg cggccggaga ggacgaccac gccgatgccg cgttcgccga
      841 gcttgccgag cagcgccgac gacagcgtca cgtcgccgcg catgaacacc cgcgacagcg
      901 gcgcgagcgg cacggtgccg atgcgctcgc cgttctcgta gaacaccagc gcctcgccgt
      961 cggctttcag cgttaccccg cgtcggtcca cgtacaggct ggtcatggct tctgctcctt
     1021 gttcaaacga tcagaaagac gtcggttacc ggctccgtgg cgcggcccag caggtcgcgc
     1081 cgcatgcgcg ggtcgagctg gaagatgtgc acgcggtctt cggcggggtc gatcagttcg
     1141 gccagcgtca gccggacttc gcgcagttcg gcggcggtca gccagcactc gaagaacgat
     1201 ttctgcccgc cgaccttgta ggccatgagg aacttgcgca cgcgcgtccg catgctcgtc
     1261 ttgcagacgt cgtagcagac cagaaacagg gttcgcgccg catcactcat gnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttccatctt gttcctggcg gtgagatcat
     1441 cctgcgcgac cggaacatgc acgtcgtggc gcagacccat tttggaacga ccgcggcatt
     1501 acccgttggc gacaaccgcc cttccgatga gttgcagaag gccctggccc agggttcgcg
     1561 gcaaggcacc tacgccagcg cttcgacaac cgccgatggc gtcagccgaa tccacgccta
     1621 tcaccgcagc gaaaagtatg gtttcaccgt gttggtgggg attccccaat cgctcatcac
     1681 cagcatctgg ctgaagcaat cggcgatcgt ggccctcctg ctcgtggcca tcctcatcgg
     1741 tttgctgctc ggaacccagc tggtttcccg ccggctggcc gatcagctgg gccgcagcgt
     1801 cagcgaagcg cgcgaagcgg aaagggaatt gttcaggacg ctgattcgct ccattcccaa
     1861 tctgatctgg ctgaagaacg ccgaaggggt ttatctggcc tgcaaccggg agttcgaaaa
     1921 atttttcggc aagagcgaag ccgagattgt cggcaagacc gattacgact tcttgtccgg
     1981 ggaactggcg gatttcttcc gcggccatga tcgtgcggcc gccgccgccg gcaagccgac
     2041 ggtcaacgag gagtggatca cctatgccga cgacggccac cgcgcgctgc tgagaaccac
     2101 caagacgccg atgatcgcgg ccgatggccg cctgatcggc gtgctcggca tcgcccatga
     2161 catcaccgag atgcgccgcc aggaagaagc gctgcgcgaa agccgcgaaa ccctcaatcg
     2221 tgcccaggcg gtggcgcatg tcgggagctg gatgctggac atcgaagcca accggctgga
     2281 gtggtcggac gaatgctatc gcctcttcgg cctgccgcca ggttcgccga tgaccctcga
     2341 gcgctttttc gcctgcatcc atcccgacga tctggagcgc gtggccgccg catggcatgc
     2401 gacgctcggc ggtgccgagt acgacatcga acatcgcatc ctcgccgccg gcgagacccg
     2461 ctgggtgcgc gagcgggcca tcatcgagcg cgatgccgac ggcaaggcgc gccgcggact
     2521 gggtacggtg caggacatca cgctgcgcag gcagaccgaa gcggcgttgc aggaagagcg
     2581 tcgggttcgc gacaccctgc tggaatcgat ccccggcgtt ttctacgcca tcgatgcaag
     2641 aggtatgttc accttctgga atcatcattt cgaacaggtg acaggacgca gcgcggagga
     2701 gttgcgccac ttcaatgcgc tggacctttt cgagggcgag gatcgcgagc acgttgccga
     2761 acgcatcgag caggtcttcc tggaaggtca gagcgatatc gaagccagcc tggtgacgaa
     2821 ggacggccag cacctcccgt attacttcac cggccggcgc atcgagatga gcgggcagcc
     2881 catcctggtt ggcgcgggta tggacatcgg cccactcaag gccgccgacg aggcgatgcg
     2941 tcgcatcaac gaagaactgg aagcgcgggt gcggcaaaac acagctgacc tgcaagccag
     3001 ctacgccaaa ctgcgcgaca ccgagttcgc catgaatcag gccgggatcg gtattgcctg
     3061 ggtgaatacg ga
//
LOCUS       DAOSIT010000265         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27702_length_3070_cov_9.618242, whole genome shotgun sequence.
ACCESSION   DAOSIT010000265 DAOSIT010000000
VERSION     DAOSIT010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27702_length_3070_cov_9.618242"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLL14_07180"
     CDS             complement(<1..169)
                     /locus_tag="PLL14_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HOG03747.1"
                     /translation="MPVFVRRLGRRDYSQTWHAMRDFTATRTEGTLDEFWCCEHAPVF
                     TLGQAGKPEHLL"
     gene            complement(247..516)
                     /locus_tag="PLL14_07185"
     CDS             complement(247..516)
                     /locus_tag="PLL14_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HOG03748.1"
                     /translation="MAEESDSLFEFPCAFPIKVMGLAADTLTRTVLDVVLRHAPDFNA
                     ATMVIRSSSAGKYLSLTCTINATSRAQLDALYRELSNHPLVKFVL"
     gene            complement(570..1703)
                     /locus_tag="PLL14_07190"
     CDS             complement(570..1703)
                     /locus_tag="PLL14_07190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG03749.1"
                     /translation="MLKLRSLLLVLLCLPVLSVAQQLPTPPALAAKSWLLVEAASGQD
                     LAAQAPEQRLEPASLTKLMTAYLSFAAIRQGTIKLDQTVPVSQKAWKTGGSRMFIQVG
                     TQVKVEDLLKGMIVQSGNDACVALAEAIAGDEENFAQMMNREAQRLGMQGSSFRNASG
                     LPDPQHYTTARDLAILAGALIRDFPEEYAKYYSMKEFRYNNITQPNRNRLLYIDPTVD
                     GLKTGHTEAAGYCLVSSAKRGSRRLLSVVLGAASDNARAQESLKLLNYGFQFYDAVQL
                     YAKNEAASSLKVWKGKESAVKIGFSNALVIAVPKGFGPKIRTELVSQQPLIAPVALGQ
                     VLATLKVSIDDKPYGEYPVLALEAVPAAGIVGRMVDTVRLWFN"
     gene            1998..2828
                     /locus_tag="PLL14_07195"
     CDS             1998..2828
                     /locus_tag="PLL14_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG03750.1"
                     /translation="MRQITATTSQILNIERVSLWGLSAGRKNLECIDLYELSRNRHSR
                     GATLQAARYPEYFRALETGEPIVADDAMKHPSTQEFTNDHLLMNGISALINSPIHADG
                     ELQGVLCIERVGPHSPWTSVQRLFSHAVANLVSVALLQHQLLSKEAELRDANSLRRAL
                     FDGARDAILISDASTGHILDANPQAERLFGRRLEELLGILQSELYASRDALDVRDLFK
                     ALAQAEGSEAVCKEVLGCDGKLIPVEITGQVIHLEKGKKIVQGVFRPLDDEESESTDV
                     "
     gene            complement(2829..>3070)
                     /locus_tag="PLL14_07200"
     CDS             complement(2829..>3070)
                     /locus_tag="PLL14_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG03751.1"
                     /translation="ASMRSVFLQLGAFASAENAESLRSHLLRELDWLSEGVQINAGGG
                     MHRVHLGPYASRADAERVAERIRLALGYKPTLVAR"
BASE COUNT          636 a          959 c          884 g          591 t
ORIGIN      
        1 acagcaggtg ctcgggcttg ccggcctgac cgagagtaaa caccggcgcg tgctcgcaac
       61 accagaactc gtcgagcgtg ccctcggtac gcgtagcagt gaagtcgcgc atcgcgtgcc
      121 aggtctggga atagtcgcgc ctgccgaggc ggcgaacgaa caccggcacc tgacccggtg
      181 cggaaaacag aacacccgca ctgtccgggc tgtccctgtc acccgcaagc ccttgctcgg
      241 cccacttcac aatacgaact tgacgagtgg gtggttgctg agttcccggt agagcgcgtc
      301 cagttgtgct ctgctggtgg cattgatggt acacgtaaga ctcaggtact tgcccgccga
      361 cgaagaacgg atgaccatcg tcgccgcatt gaagtccggc gcgtgccgga gcaccacatc
      421 gagtaccgtc ctggtcaagg tgtcggcagc cagtcccatg accttgatcg ggaacgcaca
      481 agggaattca aacaggctat cgctctcttc ggccatcgcc accaccgttg tttgcctccc
      541 gatagtcaat gagccaagcc tgcatctgcc tagttgaacc acaagcggac agtatcgacc
      601 atccggccaa caatccctgc agccggcacc gcttccagtg ccagcaccgg gtattcgccg
      661 tacggcttgt catcgatgct cactttcaat gtggcaagca cctgccccag cgctaccgga
      721 gcgatcaacg gctgctgcga gacgagttcc gttcgtatct tcggaccaaa acccttgggc
      781 acggcaatca cgagtgcatt cgaaaacccg atcttgaccg cgctttcctt gcccttccag
      841 actttgagac tggacgctgc ctcgttcttc gcatagagct gcaccgcgtc gtaaaactga
      901 aaaccatagt tgagcagttt gagcgattcc tgggcgcgtg cattgtccga tgccgcgccg
      961 agtaccactg acagcaggcg gcgagagccg cgcttcgccg acgacaccag acaataacct
     1021 gccgcctcgg tgtgacccgt cttcagacca tcgacggttg gatcgatgta caacaagcgg
     1081 ttgcgattcg gctgggtaat gttgttatac ctgaactcct tcatcgagta gtacttggca
     1141 tactcctcgg gaaaatcacg aatcagggcg ccagccagta tcgcaagatc tcttgccgtc
     1201 gtgtagtgct gcgggtcggg caagccgctg gcattgcgga agctcgaccc ctgcatgccc
     1261 aaccgctgcg cctcccgatt catcatctgc gcgaagtttt cctcgtctcc ggcaatggct
     1321 tcggccagcg cgacgcaggc atcgttgcct gactggacaa tcatgccttt cagcagatcc
     1381 tcgaccttga cctgcgtacc gacctggatg aacatccgtg agccgcccgt cttccatgcc
     1441 ttctgggaga ccggcacggt ctgatcaagc ttgatcgtac cctgccggat tgccgcgaaa
     1501 ctcagatagg cggtcatcaa cttggtcagg gaggccggtt caaggcgttg ttcgggcgcc
     1561 tgggcagcca gatcctggcc cgaagcggcc tcgacaagca accatgactt ggcggccagc
     1621 gcgggtgggg tgggcagttg ttgggcgacg gacagaactg gcaggcagag cagaacaaga
     1681 agcagggatc gaagcttgag catggcagaa tttcgcgcaa agggtgaatt atagcaagcc
     1741 acctgtgccc agcctgtgcg cgttttgtga ccacccggcg ccgagaatcg accacttcgt
     1801 gacttaccac acagcccggc ggagagtcga acacgtacca tagggcaact ttttggcacc
     1861 ccggcccacc tgccagtggg caaagcgtcc ttcccatgtt gccaccgttt cgacccatat
     1921 cgagttcgct tgacctgcag agcgcatttg cgcaactgtc aaggcgcgga ttcgccggca
     1981 accgcctgga tgagagtttg cgtcagatca ccgccacaac gagtcagatc ctcaacattg
     2041 agcgcgtcag tctgtggggg ttgtccgccg ggcggaaaaa tctggagtgc atcgacctct
     2101 acgagttgtc gcgcaaccga catagccgcg gcgctacgct acaagcggca cgctatccgg
     2161 agtacttccg ggccctcgaa acgggcgagc cgattgtcgc cgacgatgca atgaaacatc
     2221 catccacgca ggaattcacc aacgatcacc tgctgatgaa tggtatcagc gcgctcatca
     2281 attcgccgat tcatgccgat ggcgaattgc agggagtgct ctgcatcgag cgggtcggac
     2341 cccactcgcc ctggacttcc gtacaacggc tgttctcgca cgcggtcgcc aatctggtca
     2401 gcgtcgccct gctgcagcac cagttgcttt cgaaggaagc cgaactgcgc gacgcgaaca
     2461 gcctgcgacg tgccttgttc gacggcgcgc gtgatgccat cctgatctcc gacgccagta
     2521 cggggcatat cctcgatgcc aacccccagg cagaaagact cttcggacga cgcctggaag
     2581 aacttctcgg catcctgcaa agcgagttgt atgccagccg cgacgctctc gacgtgcgtg
     2641 acctcttcaa agcgctggcc caggcagagg gtagcgaggc cgtctgcaaa gaggtactcg
     2701 gttgtgacgg caagctgatt ccggtcgaga tcaccggcca ggtgatccat ctcgaaaaag
     2761 gcaagaagat tgtccagggc gtgtttcgcc cgcttgacga cgaggagtcg gagtcgaccg
     2821 acgtctgatc aacgcgccac caaggttggc ttatagccca aggccaggcg aatcctctcg
     2881 gctacccttt cggcatcggc acggctggca tagggcccca ggtggactcg gtgcatgccg
     2941 ccaccggcat tgatctggac gccctcgctc agccagtcga gttcccgaag cagatgtgag
     3001 cgcaaactct cggcgttttc agcgctggca aaagcgccaa gttgcaggaa cacgctgcgc
     3061 atgctcgccg
//
LOCUS       DAOSIT010000266         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27736_length_3067_cov_11.601594, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000266 DAOSIT010000000
VERSION     DAOSIT010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27736_length_3067_cov_11.601594"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLL14_07205"
     CDS             complement(<1..516)
                     /locus_tag="PLL14_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG03752.1"
                     /translation="MPIYAIGAKRPQIDPQSWVAPNAIVIGDVRLAKNASIWWSATVR
                     GDNDPVSIGENSNIQDNCVLHTDEGVPLSIGRDVTVGHLVMLHGCTVGDGALIGIGSV
                     LLNRAVIGKGCIVGANTLIPEGKIFPDHSLIVGSPGKVVRQLSAEEVDRLQHSADHYV
                     ANWQRYLRELQI"
     gene            complement(565..1785)
                     /locus_tag="PLL14_07210"
     CDS             complement(565..1785)
                     /locus_tag="PLL14_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG03753.1"
                     /translation="MLKAMLREAWQAMGANRLRTVLTMLGMVIGVGSVVLMMAIGQGA
                     QYAVAQTISTMGSNLYILISGSTSTGGLRSGGGGGPTLTVADAEAIAELDGVANVAPV
                     HQGTQQLVYGPNNWSSTVVGTTPPYLEARAWTIVSGHPFGDSDVRSATRVALIGQTAA
                     DNLFANDDPLGKTIRIRQSPFVIIGVLGKKGQNLDGRDQDDTLIIPLSTAQRKVFGTP
                     FAGSVRLIMVQATSAEAMASVERSMTALLRQRHRIREGQDNDFYLRNLAAAADSAAET
                     TRVMSVLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARQKDILTQFLLE
                     ALMISVAGCLIGLVLGIAGALLTNAVTDMVIVISGGSILVAFGVAAGIGIFFGYYPAH
                     RASGLDPIEALRHQ"
     gene            complement(1779..2489)
                     /locus_tag="PLL14_07215"
     CDS             complement(1779..2489)
                     /locus_tag="PLL14_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03754.1"
                     /translation="MSQVVIRTLAVGKSYRTAAGPFAALSGVNLAIEAGEFVAIMGPS
                     GSGKSTFMNLLGCLDTPSSGDYFLVGRNVAQLGKDELATLRNRTIGFVFQGFNLLPRM
                     TLADNVALPLVYAGVERDARRARALQELARVGLGDYAASLPSRISGGQQQRVAIARAL
                     VNSPQLILADEPTGNLDSHTSEEIMALFTELNREGITIVLVTHEPDIAACARRQVRFL
                     DGRLVSDVPTAARALAAC"
     gene            complement(2489..>3067)
                     /locus_tag="PLL14_07220"
     CDS             complement(2489..>3067)
                     /locus_tag="PLL14_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG03755.1"
                     /translation="SSFAEADIGSIREGQKVRFTVDAFPNRSFQGQVQQIRLNPTTQQ
                     NVVTYNVRVSLSNPEQILLPGMTAYVSIGVASRQDALLVPNAALRFKPSDIAAGDKPP
                     GGSAPPGGAKATGEGAGEAKGKGRKRDSASGTVYRIDKDELKAVSVQLGITDNRSTEV
                     LGGELKAGDTVVVSENIANTSGKPSSVGMRLF"
BASE COUNT          510 a          930 c         1039 g          588 t
ORIGIN      
        1 gatttggagt tcgcgcaggt agcgttgcca gttggcgacg tagtgatcag cactgtgctg
       61 caggcgatcg acttcttctg cgctgagctg gcgcacgact ttgccggggg agccgacgat
      121 cagcgagtgg tccgggaaga tcttgccttc gggaatcagc gtgttggcgc cgacgatgca
      181 gcccttgccg atcaccgcgc gattgagtag caccgagccg atgccgatca aggctccgtc
      241 acctaccgtg cagccgtgca gcatcaccag atggccgacc gtgacgtcgc ggccgatcga
      301 cagcggtacg ccttcatcgg tgtgcagcac gcaattgtcc tggatgttgc tgttctcgcc
      361 gatgctgacc ggatcgttgt cgccacgcac ggtggcgctc caccagatcg aggcgttttt
      421 tgcgagccgg acgtcgccga tcacgatggc attgggggcg acccagcttt gagggtcgat
      481 ctgcgggcgc ttggcgccga tggcgtagat gggcatggct tgaatctcgc ggtggttgca
      541 ggtgaacaat tgtagcctgg ccggctattg atggcgtagc gcctcgatcg gatcgaggcc
      601 ggatgcccgg tgcgccggat aataaccgaa gaagatgccg atccccgcgg cgacgccaaa
      661 ggcaaccagg atcgagccgc cggaaatgac gatcaccatg tcggtgacgg cgttggtgag
      721 cagggcgccg gcgatgccga ggacgaggcc gatcaggcaa ccggcaaccg agatcatcag
      781 cgcttcgagc agaaactgcg tcaggatgtc tttctggcgg gcgccgatgg ccatgcggat
      841 gccaatttcg cgcgttcgtt cggttaccga gacgagcatg atgttcatga tgccgatacc
      901 gcccacgagc agcgaaaccg acgcgatggc tcccagcagg accgacatca cgcgcgtggt
      961 ttcggcagcg gagtcggccg cagcagccag gttgcgcaga tagaagtcat tgtcctgacc
     1021 ttcgcgaatg cgatggcgct ggcggagcag ggcggtcatg ctcctttcga cgctcgccat
     1081 cgcttccgcc gaggtggcct ggaccatgat cagacgcacc gaacccgcaa agggcgtacc
     1141 gaagaccttg cgctgggcgg tgctcagcgg aatgatcagg gtatcgtcct ggtcgcgtcc
     1201 gtcgaggttc tgtcccttct tgccgagcac gccgatgatc acgaagggac tctggcggat
     1261 acggatggtc ttgccgaggg gatcgtcgtt ggcgaacaga ttgtcggcgg ccgtctgtcc
     1321 gatcagtgcg acgcgcgttg ccgatcggac atccgagtcg ccgaaggggt ggccgctgac
     1381 gatcgtccac gctcgtgctt cgaggtaagg cggggtggta ccgaccaccg tcgaactcca
     1441 gttgttggga ccatacacca gttgctgcgt gccttgatgc accggcgcga cgttggccac
     1501 cccgtcgagt tcggcgattg cttcagcatc agcgacggtc agcgtcgggc cgccgccacc
     1561 gccgctgcgc aggccgccgg tactggtcga cccggagatc aggatgtaca gattgctgcc
     1621 catggtgctg atggtctgcg ccaccgcata ctgcgcgccc tggccgatgg ccatcatcag
     1681 taccaccgag ccgacgccga tgaccatccc gagcatggtc aggacggtgc gcaggcgatt
     1741 ggcgcccatc gcctgccagg cctcgcggag catcgccttc agcatgcggc aagcgcgcgt
     1801 gcagccgtcg gaacgtcgct gacgaggcgg ccgtcgagga aacgtacctg ccgtctggcg
     1861 caggcggcga tgtccggttc gtgggtgacc agcacgatag tgattccttc ccggttgagt
     1921 tccgtgaaca gggccatgat ttcttcgctg gtatggctgt cgaggttgcc ggtcggttcg
     1981 tcggcgagga tcaattgtgg cgagttgacc agcgcgcggg caatcgccac gcgttgttgt
     2041 tggccgcccg agatgcgact gggcagcgat gcggcgtaat cgccgaggcc gacccgtgcc
     2101 agttcctgca gggcgcgggc tcgcctcgca tcgcgctcga cgccggcgta caccaagggc
     2161 agggcgacgt tgtcggccag cgtcatccgt ggcagcaggt tgaagccctg gaatacgaag
     2221 ccgatggtgc ggttgcgcag cgtcgccaat tcgtccttgc ccagttgggc gacgttgcgg
     2281 ccgaccagga agtagtcgcc gctgctcggc gtatcgaggc agccgagcag gttcatgaag
     2341 gttgatttgc cggatcccga ggggcccatg atggccacga attcgccggc ttcgatagcc
     2401 aggttgactc cgctcagagc cgcaaaggga ccggcggccg tgcggtacga cttgcccacg
     2461 gcaagagtgc gtatgaccac ctgcgacatc agaacagccg cattcccacg ctgctcggct
     2521 tgccggaggt gttggcgatg ttctcgctga cgacgaccgt atcgcctgcc ttcagttcgc
     2581 cgccaagcac ctcggtgctg cggttgtcgg tgattccaag ttgcacactg accgctttca
     2641 gttcgtcctt gtcgatgcgg tataccgtgc cactggcgct gtcgcgcttc ctgcccttgc
     2701 ccttcgcttc gccggcaccc tcgccggtgg ctttcgcgcc gcccggtggg gcactgccgc
     2761 ctggcggctt gtcgccggcg gcgatgtcgc tgggcttgaa acgcaaggcc gcgttcggca
     2821 ccagcagagc atcctgacgg ctggctacgc cgatgctgac gtaggcggtc atgccgggca
     2881 gcagaatctg ttcggggttg gagagcgaca cgcgaacgtt gtaggtcacg acattctgct
     2941 gggtcgtcgg gttgaggcga atctgctgca cctgcccctg gaagctgcgg ttggggaagg
     3001 cgtcgacggt gaaacggacc ttctgtccct cgcggatgct gccgatgtcg gcttctgcaa
     3061 agctcga
//
LOCUS       DAOSIT010000267         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_27780_length_3064_cov_9.663343, whole genome shotgun sequence.
ACCESSION   DAOSIT010000267 DAOSIT010000000
VERSION     DAOSIT010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27780_length_3064_cov_9.663343"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="PLL14_07225"
     CDS             <1..2115
                     /locus_tag="PLL14_07225"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOG03756.1"
                     /translation="EQLMEDLEPQAFFEAKRLEQDERHRRYQDSPYSLEPNCKEAPGG
                     LRDLQTILWIARAAGYGNSWEDLAQHGFITPAEEQQLQRSVSFLQRLRTRLHLHVNRR
                     EDRLLFDYQTALAEQLGFEPTATRRASERLMQGYYRTAKEVTQINTILLLNLGAAISP
                     PPQQLPQAINERFRNACDLLDVVDEDLFGSNPGAMLEAFLLMQRHPQLQGMTARTLRA
                     LWHARTLIDDSFRRSPDNRAYFLEILKQPRGVLHELRRMNQFGVLGRYLPNFGRIVGQ
                     MQHDLFHVYTVDQHSLQVLRNLRRFLAGEFAHEYPLCSELISDFPRPWVLYVAALFHD
                     IAKGRGGDHSELGAVDAAKFCADHGIDAEDSELIVWLVRRHLLMSSVAQKQDIADPDV
                     VGAFAATVKDERQLVALYLFTVADIRGTSPKVWNTWKGQLLEQLFRSTCRTLLAGGQV
                     PVRQGLIEERQQEALRLLRYFALPDAVHQRLWRQLDTVYFLRHSAEEIAWHTRALHYR
                     TRVEEPVVKARVNPQGEGLEVMIYTRDQRDLFARLVGFFSRAGYTIVDAKIHTTRHGY
                     ALDSFMLLDLSDRGSDRAMITYIEHELSARLSRLSPPEAPGSGRISRQVRHFPVQPLV
                     TIEPDEKGLHYVLSVSAADRPGLLYTVAMTLAAHSANLHTAKISTLGERVEDTFLLSG
                     GDLGNTASRIKLESELLERLKI"
     gene            2520..>3064
                     /locus_tag="PLL14_07230"
     CDS             2520..>3064
                     /locus_tag="PLL14_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03757.1"
                     /translation="MKFIDSYRRDETTDEDGKMATENDDTKELLLTRLNRVRSRSGEA
                     AMKSTEADGNALQTLGSSPTKTMTGNPVQLAAVQPDIRRRRLVRGAAAVAPMVLTLRS
                     GALAAASCTGAKIISIDSTGQINPAGLNDTDVCFTDQQVCPGYPLSGPTKIESGTRVG
                     QIAPTYPGGPYACGNLKSTNVA"
BASE COUNT          658 a          933 c          887 g          586 t
ORIGIN      
        1 gagcaactca tggaagacct tgagccgcag gccttcttcg aagccaagcg cctggaacag
       61 gacgagcgcc accgccgtta tcaggattca ccgtacagcc tcgaacccaa ctgcaaggag
      121 gcaccggggg gtttgcgcga tctgcagacg atcctgtgga ttgcccgcgc cgcaggctat
      181 ggaaattcct gggaagatct ggcccagcac ggtttcatca ccccggcaga agagcagcaa
      241 ctgcagcgca gcgtgagttt cctgcagcgt ctgcgaaccc gtctgcacct gcacgtcaac
      301 cggcgcgaag atcgcctcct cttcgattac cagacggcgc ttgcagaaca gttggggttc
      361 gagcccacag ccacccgccg cgccagcgag cgactgatgc aaggctatta tcgaacggcc
      421 aaggaggtca cacagatcaa caccatcctg ctgctgaacc tcggcgcagc gatatcgccg
      481 ccaccgcagc agttgccgca agcgatcaat gagcgcttcc gcaacgcctg cgatctgctt
      541 gatgtggtcg acgaggatct cttcggcagc aaccctggcg ccatgctcga ggcctttttg
      601 ttgatgcaaa ggcatcccca gctgcagggg atgacggcgc gcactttgcg tgccctgtgg
      661 catgcccgca cgctgatcga cgacagcttc cgccgctctc ccgacaatcg tgcgtatttc
      721 ctggagatcc tgaaacaacc gcgcggggta ctgcacgaac tccggcgcat gaaccagttc
      781 ggagtgcttg gcaggtacct gcccaatttc ggcaggattg tcggccagat gcagcatgac
      841 ttgttccacg tgtacaccgt cgaccagcat agcctgcagg ttctgcgcaa cctgcgccgc
      901 tttctcgccg gcgagttcgc tcacgagtat ccgctgtgca gcgagctgat cagcgatttc
      961 ccacgtccct gggtgctcta cgtcgcagcg ctgttccacg atatcgccaa ggggcgaggt
     1021 ggcgaccact cggaactcgg tgccgtcgat gccgcgaagt tctgtgcaga tcacggcatc
     1081 gacgctgagg acagcgaact gattgtctgg ttggtccggc gtcatctgtt gatgtccagc
     1141 gttgcccaga aacaggatat cgccgaccct gacgtcgtgg gtgcatttgc cgcgacagtg
     1201 aaggacgagc gacaactggt ggccctttat ctgttcaccg tcgccgacat tcggggtacc
     1261 agtcctaaag tgtggaacac atggaaaggc caactgctcg agcagctctt ccggagcacc
     1321 tgccgaaccc tgctcgccgg cggccaagta cccgtcagac aaggactcat cgaggagcga
     1381 caacaggaag cactgcgcct gctgcgctac ttcgcactcc ctgacgcggt gcatcaacgc
     1441 ttgtggcggc aactcgacac ggtgtatttc ctccgtcatt cggcggagga aatcgcctgg
     1501 cacacgcgcg ccctgcacta tcggactcgc gtcgaggaac cggtggtcaa ggcacgcgtc
     1561 aacccgcagg gcgaagggct ggaggtgatg atctacacgc gcgaccagcg cgacctgttt
     1621 gcacggctgg tggggttttt cagccgtgct ggttacacca tcgtcgatgc caagatccac
     1681 accacgcgtc acggttatgc gctggacagt ttcatgctgc tcgacctcag cgatcgcggc
     1741 agcgaccgcg cgatgatcac ctacatcgag cacgagttga gcgcgcgcct cagtcgcctc
     1801 agtccaccgg aggcgccagg cagcgggcga atctcgcggc aggtgaggca cttcccggtg
     1861 cagccgctgg tcaccatcga acccgacgaa aaaggcctgc actatgtgct ctcggtcagc
     1921 gctgccgatc gccccggcct gctgtataca gttgccatga ctttggctgc acacagtgct
     1981 aatctgcaca cggccaagat ttcgacgctc ggcgaacggg ttgaagatac ctttctgctc
     2041 agcggcggag acctcggcaa caccgccagc cgcatcaagc tggaaagcga gttgctggag
     2101 cgactcaaaa tctgagcatg tttgcgcacc ggcggcaccc cgggcttggc tgtacttcag
     2161 aaaatccttg cggcaggcac caaggcgaat cgatggcagg gcagacgatg gttcaaagtg
     2221 tcggtttttt ttacacacgt atcagccacc cccttccagc aattcacaaa gcaaggataa
     2281 tgcaatgatt tagttggttg tcttctggac acacaacgat tggtacagaa tttgcttgat
     2341 ctttgacgaa acctgactcg gaggcgcaaa acccagagcc cagatcgtcg tgattctgcc
     2401 catcgggtaa aagcgtgatg aagtccgcaa gtactccgca tggtcagcac taaaatacat
     2461 tggtgcagaa aagaatcggt ctcggtattg gcttagtgcc tgcagcggtg gagactgaag
     2521 tgaaattcat cgattcgtac cggcgggacg agacaactga cgaggatggc aagatggcta
     2581 ccgaaaatga cgatacaaag gagctgcttt tgacacggtt gaaccgcgtt cggagtcgtt
     2641 cgggagaagc tgcaatgaag agtacggaag cagatggcaa cgcgctacag accctcgggt
     2701 cctcgccgac aaaaacaatg accgggaacc ccgtgcagct tgccgctgtg cagcctgaca
     2761 tcaggagaag gcgcctggtt cgtggtgccg cggcggttgc accgatggtc ctcaccttgc
     2821 gtagtggtgc gctggcggct gcatcctgca ctggcgcaaa aatcatctcc atcgacagca
     2881 ctggccagat caatcccgcc ggtctcaatg ataccgacgt gtgcttcacc gaccagcagg
     2941 tgtgcccagg atatcccctg agcggcccta ccaagatcga atcaggaacc cgcgtcggtc
     3001 aaatcgcccc gacgtatcct ggcggtccct atgcttgcgg caacctcaag agcaccaacg
     3061 tggc
//
LOCUS       DAOSIT010000268         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28064_length_3042_cov_6.824908, whole genome shotgun sequence.
ACCESSION   DAOSIT010000268 DAOSIT010000000
VERSION     DAOSIT010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28064_length_3042_cov_6.824908"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLL14_07235"
     CDS             complement(<1..479)
                     /locus_tag="PLL14_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOG03758.1"
                     /translation="MTTDSSIAVEGAPAGAEDGQVADAPPAVASSRMVEAERIIHKDM
                     LLAMGVGVVPIPLVDFIGVTAVQLRMLRRLTELYQTPFSKRDTSALVSALVGGALPAF
                     AAWPVASLVRWIPVVGWTLGSASMVILSGASTYAVGKVFAQHFERGGSISDFDTGAA"
     gene            complement(649..2307)
                     /locus_tag="PLL14_07240"
     CDS             complement(649..2307)
                     /locus_tag="PLL14_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HOG03759.1"
                     /translation="MELIKLFKSESSKAFKLIILMAVVSGAANGALVGLINAGAMAVH
                     SETVATRDFILFVVGLVLYLYSKDISENRGKQVMELAMSKQRLRIYEKIQNASLSTIE
                     ALNPSEVIAKTTRNISQILQSSDSVIYGMQSLMMLVFCSIYLFIISAAAFLVVMAGVG
                     LLIFLRYMRDSTNKRQLDELIVREGRQSGYMADMIEGFKEIKINHAKCEDLFATFNGA
                     VQDARALSMQTAVQYIRFRIVMQASFYIILAVVVFMLPRFIDTYSRQVMESTSVVLFI
                     VGYLSGFVEIIPVFARSNAALKNMAALEAQLDAAVELRPAMLELFPGFQTISARDLHF
                     AYQDQSGEKAFSVGPLNLDVQRGEILFLTGGNGSGKSTLLKLLTGLYRPDAGAIAVDG
                     VAVNQASVFALREMYSAIFTDFHLFDRFYGYHDVDAARINALIHDLGLEQKVRFADGA
                     FSTLKLSTGQRKRLALIIALIEDRQIYVFDEWAADQDQRFRRHFYEVILPELKQRGKT
                     VIAVTHDDAYWGHADRLVKLDGGAVVGDTHAAPASREGSASNAI"
     gene            complement(2350..>3042)
                     /locus_tag="PLL14_07245"
     CDS             complement(2350..>3042)
                     /locus_tag="PLL14_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG03760.1"
                     /translation="KLIAIDLDGPLLVDTFSPIMYQLCSDYYHIDYTRALERHTFSRP
                     RAEVVAYIRQLVQATMSAQDLAKTTEQSLESYFEYRAEYLRHNPSGMRPEVPAFLDLL
                     AGLEVTLLCYGGLAEQHMREGLGTYAGHFVQYVCTNDCRPGIREIIEDIHPVEPQQAL
                     FIDDVNFVAEHARALRVPFIGVPSLAPWSWQKKDMQETGVRHIVDSVGQIDRTMLDAI
                     DREAGSGDFWWR"
BASE COUNT          612 a          950 c          911 g          569 t
ORIGIN      
        1 ttggccgcgc cggtgtcgaa atcgctgatg ctgccgccgc gctcgaaatg ctgcgcaaac
       61 accttgccga cggcataggt cgaggcgccc gacagaatca ccatcgaggc cgaacccagc
      121 gtccagccga ccaccggtat ccagcgcacc aggctggcca ccggccaggc cgcaaaggcc
      181 ggcaaggcgc cgcccaccag tgccgaaacc agtgcgctgg tatcgcgttt tgaaaaaggc
      241 gtctggtaca gttcggtcag acgacgcagc atgcgcaact gcacggcggt cacaccgatg
      301 aagtcgacaa gaggaatcgg caccaccccc actcccatcg ccaacagcat atccttgtgg
      361 atgatgcgct cggcttcgac catccggctg ctggcaacgg cgggcggcgc atccgccacc
      421 tgcccgtcct cggcacccgc tggcgcgcct tcaaccgcaa tgctactgtc tgtcgtcatg
      481 attaccgctc ctctgaatga aaaacaagcc cgccaggact tgcgccggcc tgctgccagc
      541 cgcccgtccc gccagggcga agaaattgcg gcgcaacggc gctgtcttgc ctgccgcgtc
      601 gatctgccaa cgatttcgcc ttataacaga ttcattattg atgcacactc aaattgcgtt
      661 gctggccgat ccctctcggg atgccggtgc ggcgtgcgta tcgccgacga cagccccgcc
      721 gtcgagcttg acgaggcggt cggcatgccc ccagtaggcg tcgtcatggg tgacggcaat
      781 caccgtcttg ccgcgttgct tgagttcggg caggatgact tcgtagaaat ggcgccggaa
      841 acgctgatcc tggtcggccg cccattcgtc gaagacatag atctgccgat cctcgatgag
      901 cgcgatgatc aacgccagac gcttgcgctg gccggtcgat agcttgagcg tcgagaacgc
      961 gccatcggca aagcggacct tctgttccag ccccaggtcg tggatcaggg cgttgatgcg
     1021 cgccgcgtcg acgtcgtgat agccgtagaa gcggtcgaac agatgaaagt cggtaaagat
     1081 tgccgagtac atttcgcgca gcgcgaatac gctggcctgg ttgaccgcca caccatcgac
     1141 ggcaatggct ccggcatcgg ggcggtacag gccggtcagc agcttcagca gcgtagactt
     1201 gccgctgccg ttgccgccgg tcaggaacag gatctcgccg cgctgtacat ccagattgag
     1261 cggacccacc gaaaaagcct tctccccgga ctgatcctga tacgcaaagt gcaggtccct
     1321 ggcactgatc gtctggaagc cgggaaacag ttccagcatc gccggacgca gttcgaccgc
     1381 cgcgtcgagc tgcgcctcaa gcgccgccat gttcttgagc gcggcattcg aacgggcaaa
     1441 gacggggatg atttcgacga agccggacag atagccgacg atgaacagca ccaccgaggt
     1501 gctctccatg acctgacggc tgtaggtgtc gatgaaacgc ggcagcatga agaccaccac
     1561 cgccaggatg atatagaacg aggcctgcat gacgatgcga aagcggatgt actggaccgc
     1621 cgtctgcatg ctcagggcac gcgcgtcctg gaccgcgccg ttgaaggtgg cgaacagatc
     1681 ttcgcacttg gcgtggttga tcttgatttc cttgaagccc tcgatcatgt cggccatgta
     1741 acccgactgg cgaccctcgc ggacgatcaa ctcatcgagc tgccgcttgt tggtgctgtc
     1801 gcgcatgtag cgcaggaaga tcagcaaccc gaccccggcc atcaccacca ggaaggccgc
     1861 ggctgaaatg atgaacaggt agatcgaaca aaacaccagc atcatcagcg actgcatgcc
     1921 gtagatcacg ctgtcggagg actgcaggat ctggctgatg ttgcgcgtgg tcttggcgat
     1981 cacttcgctg gggttgagcg cttctatcgt gctcaggctg gcgttctgga ttttctcgta
     2041 gatgcgcagg cgctgcttgc tcatcgccag ttccatcacc tgtttgccgc gattctcgga
     2101 aatatccttc gaataaaggt agagcaccag gccaaccacg aacaggatga agtcgcgcgt
     2161 ggcgacggtt tcggaatgga ccgccatggc acccgcattg atcaggccga ccagcgcgcc
     2221 gtttgccgcg cccgacacca ccgccatcag gatgatgagc ttgaacgcct tcgacgactc
     2281 gctcttgaat aacttgatca attccatgcc aggctggtcc ggtggttttc tggtaaacgt
     2341 ccggcggctt caacgccacc agaagtcgcc gctgccggct tcgcggtcga tggcgtcgag
     2401 catggtgcgg tcaatctggc cgaccgagtc gacgatatgc ctgacaccag tctcctgcat
     2461 gtccttcttc tgccagctcc acggcgccaa cgacggtacc ccgatgaagg gcacgcgcag
     2521 cgcccgggca tgctcggcga caaagttgac gtcgtcgatg aacaaggcct gttgcggttc
     2581 gaccggatgg atatcctcga tgatctcgcg tatcccgggg cgacagtcgt tggtgcacac
     2641 atactgcacg aagtgcccgg catacgtccc cagtccttcg cgcatgtgct gttcggcaag
     2701 gccaccgtaa cagagcagcg tcacctccag gccagccagc aggtcgagga acgccggcac
     2761 ttcaggccgc atgcccgacg gattgtggcg cagatactcg gcgcggtatt cgaaatagct
     2821 ttcgagactt tgctcggtgg tcttggccag gtcttgcgcg ctcatcgtcg cctgcaccaa
     2881 ctggcggatg taggcgacca cttcggcgcg cggacgcgag aaggtatggc gttcgagcgc
     2941 gcgcgtatag tcgatgtggt aatagtcgct gcacaactgg tacatgatcg gactgaaggt
     3001 atccaccagc agcggcccgt ccaggtcgat ggcaatcagc tt
//
LOCUS       DAOSIT010000269         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28257_length_3028_cov_6.323579, whole genome shotgun sequence.
ACCESSION   DAOSIT010000269 DAOSIT010000000
VERSION     DAOSIT010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28257_length_3028_cov_6.323579"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..879)
                     /locus_tag="PLL14_07250"
     CDS             complement(94..879)
                     /locus_tag="PLL14_07250"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03761.1"
                     /translation="MGLGLNLEGVSFAYDATPVLQDIHVNIPEGQFVSLLGASGSGKT
                     TLLRLAAGLNEVSSGRLSWNGVPVRGPSLERGVVFQDYALFPWLSVVDNVEIAIAKAK
                     PGIRKAERLSEARGYLRQLGLAAAIGKYPGELSGGMRQRAAIARALALGSPLLLMDEP
                     FGALDPVNRARLQDLLLASWELATPRKTIVFVTHDVDEAIYLGDRLIVLGSSPGRLIA
                     DLPVPLARPRERRSLLDSHSLHALREQVAATLVQDTLQRLRAA"
     gene            complement(879..1901)
                     /locus_tag="PLL14_07255"
     CDS             complement(879..1901)
                     /locus_tag="PLL14_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG03762.1"
                     /translation="MISFRTGRQLLAALVLVAGLGNAVADTLKVGYLPVTGHAKFFIA
                     KEQGLFAREGLDVELIEFQNSADGLNAIVAGKLDIGAFGTTAPVAHIAKGAKIKIIGG
                     IMGGDAALIATPANAGKIAGVADLRGKKIATVRMASGDAVLRGALKDKGINWKNDVQI
                     FELKNPAAVIEAVKSGQVDAGLVWGPHDLRAEDQGLRVVIHSTDLQPGHPCCRLVVTE
                     ASLKNPVTWEKFLRAILQAEKFAAENKKETIDSIARYVKIDRQLLWRGYYSQYLDQSS
                     DPDVKGVKHFAEVMLNSEFITAKPDLDAAIDVRVYEKVLNQLARENPGDPFWKKLQRE
                     FRQKDA"
     gene            2107..>3028
                     /locus_tag="PLL14_07260"
     CDS             2107..>3028
                     /locus_tag="PLL14_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG03763.1"
                     /translation="MTGHLKIQSISSLVAIAFCNAPAAEENIAPLSTIEVRGDVDNGY
                     VAKRANSATKTDTPILDTPMAIQVITRDVLDDQQTLSIQEAVKNVSGVQTPPGVYYDN
                     FLIRGFSTGSDTFRNGLKLNGVIGTEDMAFVDHVDVVKGPASMLYGRIQPGGLVNIVT
                     RKPQDETAASLQQQVGSWGLLRTTADITGPANKDKTLLYRVMGVYDQADNFIDYQHHK
                     NAAGAAYLSWLPSSSFQANLQLEYYDQKVANPGYTAQQIPIIGNRPADVPRHWTQNDP
                     VMWSQWPNTVKRTTAAFDWTYAFNDSWKLTQ"
BASE COUNT          595 a          968 c          880 g          585 t
ORIGIN      
        1 cgatatagtc gctcatcggt ggttctcctc gtactttcag gtcgcggccg aacagcgggg
       61 tcagggcgtc acgactcggg cacatgtcct gtttcaggca gccctgagac gctgcagggt
      121 atcctgcacc agcgtcgcgg ccacctgttc ccgcagtgca tgcagactgt ggctgtcgag
      181 cagactgcgg cgctcgcgtg gccgcgccag cggcaccggc aggtcggcaa tcagccggcc
      241 gggcgacgat ccgaggacga tcaggcggtc cccgaggtag atcgcctcgt ccacgtcatg
      301 cgtgacgaaa acgatggtct tgcgcggtgt cgcgagttcc cagcttgcca gcagcagatc
      361 ctgcaagcgc gcccggttca ccggatcgag cgccccgaag ggttcgtcca tcaacagtag
      421 cggcgaaccc agggccaatg cacgggcaat ggcggcgcgc tgacgcatgc cacccgacag
      481 ttcgccaggg tatttgccga tcgccgcggc cagcccaagt tgcctgaggt agccgcgcgc
      541 ctcactcagg cgctcggctt tccggatgcc gggcttggcc ttggcaatgg cgatctcgac
      601 gttatcgacc accgacagcc agggaaacag cgcgtagtcc tgaaaaacca cgccgcgctc
      661 aaggctgggg ccgcgcaccg gtaccccgtt ccaggacaac cggccgctgg agacctcgtt
      721 cagaccggcc gccagtcgca gcaaggtggt tttccccgac ccgctggccc ccagcaggct
      781 gacgaactgg ccttccggga tattgacatg gatatcctgg agaaccggtg tcgcatcgta
      841 ggcgaagctg acaccctcca gattgagacc gagtcccatc aggcgtcctt ctgccggaat
      901 tccctctgca gcttcttcca gaagggatcg ccggggttct ccctggccaa ctggttcaac
      961 accttttcgt agacccgcac gtcaatcgcg gcgtcgaggt cgggcttggc ggtgatgaac
     1021 tcgctattca acatcacctc ggcgaaatgc ttcacgccct tgacgtcggg atcgctggac
     1081 tggtcaagat attgcgagta gtagccgcgc cagagcaact ggcgatcgat cttgacgtag
     1141 cgggcaatcg aatcgatggt ttccttcttg ttctcggccg cgaatttttc ggcctgcagg
     1201 atggcacgca ggaatttctc ccaggttacc gggttcttca ggctggcctc ggtgaccacc
     1261 agacggcagc acggatgacc gggttgcagg tcggtggagt gaatgaccac ccgcagcccc
     1321 tggtcttcgg cccgcaggtc atgcggtccc cacaccagtc cggcgtcgac ctggcccgac
     1381 ttgaccgcct cgatcaccgc cgcgggattc ttcagttcga agatctgcac atcgttcttc
     1441 cagttgatgc ctttgtcctt gagagcgccg cgcagcacgg catcgcccga agccatgcgc
     1501 acggtggcga tcttcttgcc ccgcagatcg gccacgccgg cgatcttgcc ggcattcgcc
     1561 ggcgtggcga tcagggcggc atcaccgccc atgatgccgc cgatgatctt gatctttgcc
     1621 cctttggcaa tgtgggccac cggtgccgtg gtcccgaagg cgccgatgtc gagtttgccg
     1681 gcaacgatgg cgttgagtcc atccgcggag ttctggaact cgatcagttc gacgtccaat
     1741 ccttccctgg caaacaggcc ctgctccttg gcaatgaaga acttggcatg gccggtgacc
     1801 ggcagataac cgaccttgag ggtatccgcc acagcgttgc cgaggcctgc gaccagcacg
     1861 agcgccgcca gaagctgtcg ccctgtcctg aaactgatca tcgttctctc ctgaagacgt
     1921 gaaaattcac aacgggaatg taaaccctgc tcccagctgt agaaaacgat gcatttcttc
     1981 gatccttata cccccaccgg ttcgggtctc gacacccctc gtcgctgtca cctgcggcaa
     2041 ttggtcactt ccgccatgcc caaaccaaat gctatcttgc cgcgctttac cgtttgggaa
     2101 tcagtcatga ccggacacct gaagattcag tcgatctcgt cactcgtcgc gatcgctttt
     2161 tgtaatgctc ctgctgcgga agaaaacatt gcgccgctat cgacgatcga ggtgcgcggc
     2221 gatgtcgaca acggctacgt cgccaagcga gccaactccg cgacaaagac cgatacgccg
     2281 attctggaca ctccgatggc aatccaggtg atcacacgcg acgtgcttga cgaccagcaa
     2341 accctgtcga ttcaggaagc ggtgaagaac gtcagtggcg ttcagacgcc gcctggtgtc
     2401 tattatgaca atttccttat ccggggcttt tcgaccggca gcgatacctt ccgcaacggg
     2461 ctgaagctca atggggtcat cggaacggaa gacatggctt tcgtcgacca tgtcgacgtc
     2521 gtcaaagggc cggcgtcgat gctttatggg cgcatccaac ccggcggact ggtgaacatc
     2581 gtgaccagga agccgcagga cgaaacggct gccagcctgc aacagcaagt ggggagttgg
     2641 gggctgctcc gcactaccgc cgacatcact ggtccggcga acaaggacaa gacgctgctg
     2701 taccgcgtca tgggggttta cgaccaggcc gacaacttca tcgactacca gcatcacaag
     2761 aatgccgctg gcgcagcgta tttgagctgg ctcccctctt ccagctttca ggccaacctg
     2821 caactggaat actacgacca gaaggtcgcc aaccccggat acacggccca gcagatcccg
     2881 atcattggca atcggcctgc cgacgtaccg aggcactgga cgcagaacga tccggtcatg
     2941 tggtcgcagt ggcccaatac cgtcaagcgg acgacagcgg cattcgactg gacctacgcc
     3001 ttcaatgact cctggaagtt gacgcaac
//
LOCUS       DAOSIT010000270         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28439_length_3013_cov_8.856322, whole genome shotgun sequence.
ACCESSION   DAOSIT010000270 DAOSIT010000000
VERSION     DAOSIT010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28439_length_3013_cov_8.856322"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="nifM"
                     /locus_tag="PLL14_07265"
     CDS             complement(<1..770)
                     /gene="nifM"
                     /locus_tag="PLL14_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HOG03764.1"
                     /translation="MDSNVYLSLKLAAKLFGKPLESLAPDELHRVHRVAARQSAIETR
                     ILATPEAASVILSDDSIDASLSEIRGRYGNDQDYQTDLERIGLDVHSLRQAVARDMVV
                     EAVLERIAAQAAAVSEIDVEIFWYLHKDRFRRAEMRVLRHILVTINEQLAGNDRQSAR
                     IRIEAIRTRLLKEPQRFAEQALKHSECPTAINGGLLGTVPRGQLYPQLEATAFALDAA
                     MLSDVVESKLGYHLIYCEAIHRERLLSFAEARNTIREYL"
     gene            complement(763..1221)
                     /locus_tag="PLL14_07270"
     CDS             complement(763..1221)
                     /locus_tag="PLL14_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOG03765.1"
                     /translation="MLPRWDYGDAVRVTRNVRNDGTFPGARTGDLLVRRGRVGHVRDV
                     GTFLQDQIIYSVHFLDEGRVVGCREEELIGVDEPWIDSRFEVRQKVRATRSLVIRGEI
                     RVPLGSRGEILDLERSAAQGVVYHTHFDCLPGNPLLVPEGALGLWESDDG"
     gene            complement(1226..1540)
                     /locus_tag="PLL14_07275"
     CDS             complement(1226..1540)
                     /locus_tag="PLL14_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HOG03766.1"
                     /translation="MPCETDSFEDDLADLSSAEEFLEYFDIDFDVRVVQVNRLHILQR
                     FHDYLAKQEAGKAPDYAAYRQWLARAYADFVDSSAQAEKVFAVFQKAEGSSFVPLSSL
                     TD"
     assembly_gap    1564..1663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1664..2301)
                     /gene="cysE"
                     /locus_tag="PLL14_07280"
     CDS             complement(<1664..2301)
                     /gene="cysE"
                     /locus_tag="PLL14_07280"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOG03767.1"
                     /translation="MTDLSTPLLHTVRPSQTPPGLRALFAEDLACVFDRDPAARSRIE
                     VLLTYPGLHAILVHRLAHRLWRRGWGFSARWLAFIGRWLTNVDIHPGATIGRRFFIDH
                     GAGVVIGETAEIGDDCTLYHGVTLGGTTWNKGKRHPTLGNGVVVGAGAKILGAIEVGD
                     GANVGANSVVVKEVPPGRTAIGIPARLVGGRSKVGAVGTAGMAINLDHHLIP"
     gene            complement(2309..>3013)
                     /locus_tag="PLL14_07285"
     CDS             complement(2309..>3013)
                     /locus_tag="PLL14_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOG03768.1"
                     /translation="EDASRGEPDLLAAIAEAAQSAGAQRIRFADTLGVLDPFATFERI
                     RHLRARCDLEIEMHAHDDLGMATANTLAAARAGATHLNTTVNGLGERSGNAPLEEVVL
                     GLHVCHGIETGIDLAGFPDISGRVAIASGRPLCWQKSIVGEGAFTHEAGIHVDGLLKD
                     PANYQGFDPCLVGRQHRVVLGKHSGARAVQQVMASLGHALSDAAARAFLQRLRGFMTH
                     HKQVPSEADLLKLLDR"
BASE COUNT          487 a          910 c         1000 g          516 t
ORIGIN      
        1 agatactcgc gaatggtgtt gcgggcctcc gcgaagctca gcaggcgctc gcgatggatg
       61 gcttcgcagt agataaggtg gtagcccagt ttgctctcga ccacgtcgga gagcatggcg
      121 gcgtcgaggg cgaatgcggt ggcttcgagt tgcggataga gctggccgcg cggcaccgtg
      181 ccgagcaggc cgccgttgat cgcggtcgga cactcagaat gcttcagcgc ctgttccgcg
      241 aagcgctgcg gttccttgag caggcgcgtg cggatggcct cgatcctgat gcgggcggac
      301 tgccgatcgt tgcccgccag ttgctcgttg atggttacca ggatgtgccg cagcacgcgc
      361 atttcggcgc gtcgaaaacg atccttgtgc aggtaccaga agatctcgac atcgatctcg
      421 ctgaccgctg ccgcctgcgc cgcaatcctt tccagtaccg cttcgacgac catgtcgcgg
      481 gcgacggcct ggcgcaggga gtggacgtcg agaccgatgc gttcgaggtc ggtctggtag
      541 tcctggtcat tgccgtagcg gccacggatc tcgctcaggc tggcatcgat cgagtcgtcc
      601 gacaggatca cgctggcggc ttccggcgtt gccaggatcc gggtctcgat cgcggattgt
      661 ctggcggcga cccggtgtac cctgtgcaac tcgtcgggcg ccagcgattc caggggtttg
      721 ccgaacagtt tcgcggccag cttgagcgag agatagacgt tgctatccat cgtcgctctc
      781 ccataatccg agcgcgccct cggggacaag caggggattc cccggcaggc agtcgaagtg
      841 ggtgtgataa acgacgccct gcgccgcgga tcgttcgaga tcgagaatct cgccacggct
      901 gcccagcggc acccggatct cgccgcggat gaccagcgag cgcgtcgcgc gtaccttctg
      961 gcgaacctcg aagcggctgt cgatccaggg ttcgtcgacc ccgatcagtt cttcttcgcg
     1021 acagccgacg acgcggccct cgtcgaggaa gtgcacggag tagatgatct ggtcctgcag
     1081 aaacgtgccg acatcgcgaa catgcccgac gcgaccgcgc cgaaccagca gatcgccggt
     1141 tctggcgccg gggaaggtgc catcgttgcg cacgttgcgg gtcacgcgca ccgcgtcccc
     1201 gtagtcccag cgcggcaaca cggcttcagt ccgtcagcga ggacaggggc acgaaggacg
     1261 agccttcggc tttctggaac acggcgaaga ccttctcggc ctgcgcgctg gagtcgacga
     1321 agtccgcgta ggctctcgcc agccactggc ggtaggccgc gtagtcgggc gccttgccgg
     1381 cttcctgttt ggccaggtaa tcgtggaagc gctgcaggat gtgcaggcgg ttgacctgga
     1441 ccacgcgcac atcgaagtcg atgtcgaagt actccagaaa ttcttcggcc gaactcagat
     1501 cggcgaggtc gtcctcgaag ctgtcggttt cgcagggcat ggtcttctcc ggtttcagtc
     1561 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgggaa tcagatggtg
     1681 gtcgagattg atggccatcc cggcagtgcc caccgcgccg accttgctgc gcccgccgac
     1741 cagtcgtgcc gggatgccga tggcggtacg gcccggtggg acttccttga cgaccaccga
     1801 attggcgccg acgttcgcgc cgtcaccaac ctcgatggca ccgagaatct tggcgccggc
     1861 accgaccact accccgttgc cgagggtcgg atgccgttta cccttgttcc aggtcgttcc
     1921 tcccagcgtg acgccgtggt acaaggtgca gtcgtcgccg atctcggcgg tttcgccgat
     1981 gaccacgccg gcgccgtgat cgatgaagaa gcggcgacca atggtggcgc cgggatggat
     2041 gtcgacgttg gtcagccagc gcccgatgaa ggccagccag cgcgcgctga atccccagcc
     2101 ccggcgccag aggcgatgcg cgaggcgatg cacgaggatg gcgtgcaggc cgggataggt
     2161 cagcagcact tcgatgcgcg aacgcgccgc cgggtcgcga tcgaaaacgc aggccaggtc
     2221 ttccgcgaac agcgcccgca aacccggcgg ggtctggctt ggcctgacgg tgtggagcaa
     2281 cggcgtcgag aggtcggtca tcatcgggtc agcgatcgag cagtttgagc aggtctgcct
     2341 cggagggcac ctgcttgtgg tgggtcatga agcctcgcag ccgctgcagg aaggcacgtg
     2401 ccgcggcgtc cgacagggcg tggccgaggc tggccatgac ttgttgtacg gcacgtgctc
     2461 cggagtgctt gccgagaacg acgcgatgct ggcgtccgac cagacaggga tcgaagccct
     2521 ggtagttggc cgggtccttg agcagtccgt cgacgtggat gccggcctcg tgggtgaaag
     2581 caccttcgcc gacaatactt ttctgccagc acagggggcg tcccgaagcg atggcgacgc
     2641 gacccgaaat gtccgggaat ccggccagat cgatgcccgt ctcgatgcca tggcagacgt
     2701 gcagaccgag cacgacttcc tcgagcgggg cattgcccga gcgctcgccg agaccgttca
     2761 ccgtcgtgtt gagatgcgtc gcgccggcgc gtgcggcggc cagcgtgttg gccgtggcca
     2821 ttccgagatc gtcgtgggca tgcatctcga tttccaggtc gcagcgtgcg cgcaggtggc
     2881 ggatgcgttc gaaggtcgcg aaggggtcga gcacgcccag cgtatcggca aaacggatgc
     2941 gctgggcacc cgccgactgc gccgcttcgg cgatcgccgc caggagatcc ggctcgccgc
     3001 gcgaagcgtc ctc
//
LOCUS       DAOSIT010000271         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28586_length_3001_cov_10.482010, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000271 DAOSIT010000000
VERSION     DAOSIT010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28586_length_3001_cov_10.482010"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2796
                     /locus_tag="PLL14_07290"
     CDS             <1..2796
                     /locus_tag="PLL14_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG03769.1"
                     /translation="DGRYDDLFTSNYVTLNVLDRLKRLPGTTNVQIFGAKDYAMRIWL
                     KPDRLAQLKLTPGDVANAINEQNAQFAAGKVGQSPIGKGQDLVYTITSKGRLADPAQF
                     REIIVRANPDGSTVRLADVARVELGSRDYEFNGRYNGRPATLVGIFLSPGANALDVAK
                     SVQTTVDELQQRFPEGLSYAVPYDTTRFVRVSIEEVLKTLGEAMLLVFLVVYLFLQSW
                     RAAIIPFAAVPVSLIGTFAGIYMLGYSINTLTLFGMVLAIGIVVDDAIVVLENVERIM
                     REEGLPPRDAAVKAMHEVTGPIIAIVLTLTAVFVPIAFLGGLTGELYRQFAVTISISV
                     VLSGIVALTLSPALCVIMLKHGHRQPNRFFRWFNGWFARVTRHYLDGVVWVIRRAALV
                     LTLFAGMVAITAFLWRTTPGSLVPDEDQGFYIGAVILPDGATLERTTRVVEQVEAAVR
                     SNPDNQDVVAFAGFDFIGGGFRNNAATLFVTQKPWHERQTTSQQLVGEMFMKTAGIKE
                     ALVLAFNPPAIFGLGTAGGYEFFIQNRGDGGSKRLQEVLQQFLARANSDPQLGGAQTL
                     WRATVPQLFVDVDREKAKKAGVPIDEVFAALSSTLGTYYVNDFNKYGRTWQVLMSAEP
                     EYRRRPDDIQGLYVRSQKGEMVPLAALVNVRHSSGPDSLDRFNNLPAVKIIGQTAPGI
                     SSGQGIARVEAIAREVLPPDFSFDWGGSSYQEKKSSGASTFALGLAVVMVFLILAAQY
                     EKWSLPLCVLLALPFGTFGALVAIWAKNLLGPFFGAAPLTNDVYFQIGLVTLLGLAAK
                     NAILIVEYAVLKHAEGLSASASAIEAARLRFRPILMTSLAFILGVLPLAISTGAGAGA
                     RHSVGTGVMGGMIAATLLAVFHVPLFFKVLYDRSVREQRSHDELLDEVRHLHDVPQAS
                     PPPPDMPGSSQGGGGHD"
     gene            2789..>3001
                     /locus_tag="PLL14_07295"
     CDS             2789..>3001
                     /locus_tag="PLL14_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03770.1"
                     /translation="MTDFRASLRRQPALALAAAVTLALAACQTTPSAPPVLDLPTATA
                     TFALDLERWWANFNDPTLTTLIDEALA"
BASE COUNT          485 a         1051 c          939 g          526 t
ORIGIN      
        1 gacggccgtt acgacgacct gttcacctcg aactacgtga cgctgaacgt gctcgaccga
       61 ctgaagcgac tgcccggcac gaccaatgtg cagatcttcg gcgccaagga ctacgccatg
      121 cgcatctggc tgaagccgga tcgcctcgcg cagttgaagc tgacgcccgg tgacgtcgcc
      181 aacgccatca acgaacagaa cgcgcaattc gccgccggca aggtcggtca gtcgccaatc
      241 ggcaagggcc aggatctggt ctacacgatt acctccaagg ggcgcctggc cgacccggcg
      301 cagttcaggg agatcatcgt tcgcgccaac cccgacggtt ccaccgttcg cctcgccgat
      361 gtcgcgcgcg tcgaactggg ttcccgcgac tacgaattca acggccgcta caacggccgg
      421 ccggcgacgc tggtcggcat cttcctgtcg cccggcgcca acgccctcga cgtggcgaag
      481 agcgtgcaga ccaccgtcga cgaactgcag cagcgcttcc ccgagggcct cagctacgcc
      541 gtgccgtacg acaccacgcg cttcgtcagg gtgtcgatcg aggaggtgct gaagacgctc
      601 ggcgaagcga tgctgctggt cttcctggtg gtgtacctgt tcctgcagag ctggcgcgcg
      661 gcgatcatcc ccttcgccgc cgtaccggtg tcgctgatcg gcaccttcgc cggcatctac
      721 atgctcggct attcgatcaa caccctgacc ctgttcggca tggtgctggc cattggcatc
      781 gtcgtcgacg acgccatcgt cgttctggaa aacgtcgagc ggatcatgcg cgaggaaggc
      841 ctgccgccgc gcgacgcggc ggtcaaggcg atgcacgagg tgaccgggcc gatcatcgcc
      901 atcgtgctga cgctgaccgc ggtgttcgta ccgatcgcct tcctcggcgg gctgaccggc
      961 gaactctacc gccagtttgc ggtgaccatt tcgatctcgg tggtcctctc gggcatcgtc
     1021 gcactgacgc tgtcgccggc gctgtgcgtg atcatgctca aacacggcca tcgccagccg
     1081 aaccgcttct tccgctggtt caacggctgg tttgcgcgcg tcacccggca ctacctcgac
     1141 ggcgtcgtct gggtgatccg ccgcgccgcg ctggtgctca cgctcttcgc cggcatggtg
     1201 gcgatcaccg ctttcctctg gcgcacgaca cccggcagcc tggtccccga cgaggaccag
     1261 ggcttctaca tcggcgctgt catcctgccc gacggcgcca ccctcgagcg caccacccgg
     1321 gtcgtcgagc aggttgaggc ggcggtgcgc agcaaccccg acaaccagga tgtcgtcgcc
     1381 ttcgccggct tcgacttcat tggcggcggc ttccgcaaca atgcggcgac gctgttcgtg
     1441 acgcagaaac cgtggcacga acgccagacc acttcgcagc aactggtcgg cgaaatgttc
     1501 atgaagaccg cgggcatcaa ggaagcgctg gtactggcct tcaatccgcc ggcaatcttc
     1561 ggcctcggga ccgccggcgg ctacgaattc ttcatccaga accgcggcga cgggggttcg
     1621 aagcggctgc aggaagtcct gcagcagttt ctcgcacgcg ccaacagcga cccgcaactc
     1681 ggcggtgcac agaccttgtg gcgcgccacc gtgccgcagc tcttcgtcga cgtcgaccgc
     1741 gagaaggcga aaaaggctgg cgtgccgatc gacgaggttt tcgcggcgct gtcgtccact
     1801 ctcggcacct actacgtgaa cgatttcaac aagtacggcc gcacctggca ggtcctgatg
     1861 tccgccgaac ccgaataccg ccggcggccg gacgacatcc agggcctcta cgtgcgctcg
     1921 cagaagggcg agatggtgcc gctggcggcg ctggtcaacg tccggcactc ctccgggccg
     1981 gattcactcg accgcttcaa caacctgccg gcggtcaaga tcatcggcca gacggcgccc
     2041 ggcatcagct ccggccaggg catcgcgcgc gtcgaagcga tcgcccggga ggtattgccg
     2101 ccggacttca gcttcgactg gggcggttcc tcgtaccagg agaagaaatc aagcggtgcc
     2161 tcgacctttg cgctcgggct ggcggtggtg atggtcttcc tgatcctcgc cgcgcagtac
     2221 gagaagtggt cgctgccgct gtgcgtactg ctcgcgctgc ctttcggcac cttcggcgca
     2281 ctcgtggcga tctgggccaa gaacctgctc ggaccgttct tcggcgcggc gccgctgacc
     2341 aacgacgtgt attttcagat cggcctggtg accctgctcg gtctggccgc aaagaatgcg
     2401 atcctgatcg tcgaatacgc ggtgctcaaa cacgccgagg ggctgtccgc ctcggcctcg
     2461 gcgatcgagg ccgcacggct gcgcttccgg ccgatcctga tgacctcgct ggccttcatc
     2521 ctcggtgtcc tgccgctggc catctcgacc ggcgccggcg ccggtgcgcg ccactcggtg
     2581 ggtaccggcg tcatgggcgg catgatcgcc gccaccctgc tcgccgtctt ccacgtgccg
     2641 ctgttcttca aggtgctcta cgaccgtagc gttcgcgaac agcgctcgca cgacgaactg
     2701 ctggacgagg tcaggcatct gcatgacgtc ccgcaggcgt cgcccccgcc accggacatg
     2761 ccgggtagca gccagggagg cggcggccat gactgatttt cgagcatcgc ttcggcgcca
     2821 gcctgcgctg gccctcgccg cggcagtgac cctcgcgctc gccgcctgcc agacgacgcc
     2881 gagtgccccg ccggtcctcg acctgccgac ggcgacggca accttcgccc tggatctcga
     2941 acgctggtgg gcaaacttca acgacccgac gctgacgacc ctgatcgacg aggcgctggc
     3001 c
//
LOCUS       DAOSIT010000272         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28597_length_3000_cov_8.847199, whole genome shotgun sequence.
ACCESSION   DAOSIT010000272 DAOSIT010000000
VERSION     DAOSIT010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28597_length_3000_cov_8.847199"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..909
                     /locus_tag="PLL14_07300"
     CDS             115..909
                     /locus_tag="PLL14_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOG03771.1"
                     /translation="MRSVAVIVTLFVASLIVGCGLLPEDKDETAGWSANKLYAEAKDA
                     LNESSYAKAIKYFEKLESRYPYGRYAQQAQIDIAYAYWKDQEPASAIAACDRFIKLHP
                     NHPNVDYVYYLRGLINFNENLGIMGAISNQDMTERDPKGARESFDAFRELVTRFPESK
                     YTPDAILRMKYLVNALASLELHVARYYMKRGAYLAAANRAQYTVTNYPGAPATEEALF
                     IMVKAYDALTLNDLRDDAERVMRKNFPNSEYYVRGLERKEPWWKLW"
     gene            complement(946..>3000)
                     /locus_tag="PLL14_07305"
     CDS             complement(946..>3000)
                     /locus_tag="PLL14_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG03772.1"
                     /translation="AHAAGIIHRDLKPSNILIDDDGVPRVMDFGIAARTEGLTDDRGQ
                     LIGTPAYMAPEYILRRSSSARSDIFSAGLVLYELLTGQRAVPGTDSAEVMKRIASEDI
                     RLSQDARNLLEERLANLVYRALERDPENRYESAVQMREALDDYLVPENLAPSTDPRQS
                     TIDFLLRRMRHKSDFPALSEAVGAINRITTSENQSISEVSNTILKDFSLTNKILRMVN
                     SAYYQQAGGGNISTVSRAVVVLGLDAVRSIAITVMLFDHLQNKENASQLKDEFLRANL
                     AGVLAKDIAGKVSARADIEQVFICSMFHNLGRLLSQYYFPEESAEIRRLLLQKNSSEE
                     AAALQVLGISFEELGVGIAQAWGFPKLLVNSMRKLPAGSIRRPANTEDRLRILAAMSN
                     EICTVITDVPADQQQKELRKLATRFGEVVALDEQDLHDTLERSFEQVGQFAGIIHLNL
                     QQTRLGRQIKAWTGTSVNLPSMQVSPDAEDGLAGTVLSDALAAEVACDDVADEANLAT
                     EDIAAVVSEAILLAGIQDVSNALVSDFKLNDVLRIILETIYRAKGFKRVILCIRDGRS
                     NAMLGRFGFGPDALEIARSFKFSLVFTPDIFHAALANGVDILISDTNDPKIAARIPGW
                     FRKAVAAETFLVFPLCIKGSGVAMIYADRAQAGEIVISEKELSLLRTLRNQALLAIKQ
                     SI"
BASE COUNT          610 a          922 c          856 g          612 t
ORIGIN      
        1 ccgcagtctc tgggcacgct cagggtgatt cgggggtttg agctataatc gccgtgcccg
       61 atgtcctgga tagcggcgtc ggctacagcc ttccttttct ttgggttttc catcatgcgt
      121 agtgtagcgg ttatcgtcac tctttttgtc gcttctctga tcgttggctg tgggctactc
      181 ccggaagaca aggatgagac ggctggctgg tcggcaaaca aactgtatgc ggaagccaag
      241 gatgcgctga acgaaagctc ttacgccaag gccatcaagt atttcgagaa gctggaatcc
      301 cgctatccct acgggcgcta cgcacagcaa gcacagatcg acatcgccta cgcttactgg
      361 aaggaccagg agccggcgtc ggcgattgcc gcctgcgacc gtttcatcaa gctgcatccc
      421 aatcacccga acgtcgacta cgtgtactac ctgcgcgggc tcatcaattt caacgaaaac
      481 ctgggcatca tgggtgccat cagcaaccag gacatgaccg aacgcgatcc gaaaggtgcc
      541 cgcgagtcgt ttgatgcctt ccgggaactg gtgacacgct ttccggagag caagtacaca
      601 cccgatgcga tcctgcggat gaagtatctg gtcaatgccc tggcctcgct ggaactgcat
      661 gtcgccagat attacatgaa acgcggtgct tatctggctg cagccaaccg tgcccagtac
      721 acagtcacca actatcccgg ggcgccggcc accgaagagg cgctcttcat catggtcaag
      781 gcctatgatg cgctcacact gaacgatctg cgcgatgacg ccgagcgcgt catgcgcaag
      841 aactttccca acagcgaata ctacgtgcgc gggcttgagc gcaaggaacc gtggtggaaa
      901 ctctggtagg ccgccagacc tgatacctga tcgcaaacgg ctgaatcata tggactgctt
      961 gatggccaga agcgcctgat tgcgcaaggt cctcagcaag gacaattcct tctcggaaat
     1021 gacgatctcg cccgcctgtg cgcggtcggc gtagatcatg gccaccccgc ttcccttgat
     1081 gcagagcgga aagaccagga aagtttcggc agccaccgcc ttgcgaaacc acccggggat
     1141 gcgcgccgcg atcttcgggt cgttggtgtc gctgatcagg atgtcgacgc cattggcgag
     1201 cgccgcatga aagatatcgg gcgtaaagac cagtgaaaat ttgaaactcc tggcgatctc
     1261 cagggcatcg ggcccaaacc cgaaacgacc cagcatggcg ttgctgcgac cgtcacggat
     1321 gcacaggatt acacgcttga agcccttcgc ccggtagatg gtctcaagaa tgatccgcag
     1381 cacgtcattg agtttgaagt cactgaccaa ggcgttgcta acgtcctgaa tccctgccag
     1441 aagaatggct tcgctcacca ccgccgcgat atcttccgtg gcgaggttgg cctcatcggc
     1501 aacgtcatcg cacgccacct cagccgccag ggcgtccgag agcacggtgc ccgccaaacc
     1561 gtcctccgca tcaggtgaca cctgcatgct tggcaggttg accgaagtcc ctgtccaggc
     1621 cttgatctgc ctgccgagcc gcgtctgctg cagattgagg tggatgatgc cggcaaactg
     1681 tcccacctgt tcaaacgacc gctccagcgt gtcgtgcaga tcctgctcgt cgagcgcgac
     1741 aacctccccg aaccgcgttg caagcttgcg cagctccttc tgctgctgat cggccgggac
     1801 atctgtgatg accgtgcata tctcgttcga catcgccgcg agaatgcgca ggcgatcctc
     1861 ggtattcgcg ggacggcgaa tgctgcctgc cggcagcttg cgcatgctgt tgaccagcag
     1921 cttcggaaag ccccacgcct gggctatacc gacaccgagt tcctcgaacg agatgcccag
     1981 tacctgcagt gccgcagcct cttcggacga gttcttctgc agcagcaggc gccggatttc
     2041 ggcgctctct tcaggaaagt agtactggct caacaggcgg ccaaggttgt ggaacatgga
     2101 gcagatgaac acctgctcga tatccgcgcg tgcagaaacc ttgccggcga tgtccttggc
     2161 caggaccccg gcgagattgg cgcgcaggaa ttcatccttg agctggctgg cattctcctt
     2221 gttctgcaga tggtcgaaga gcatcaccgt aatcgcaatg ctgcgcacgg cgtcgaggcc
     2281 cagaacaacc actgcgcgcg agacggtact gatgttcccc ccaccagctt gctggtaata
     2341 ggcggagttg accatccgca ggatcttgtt ggtcaacgag aaatccttga ggatcgtgtt
     2401 cgaaacctcg gaaatactct gattctcaga cgtcgtgatc ctgttgatcg ccccgaccgc
     2461 ctccgacaag gccggaaaat cgctcttgtg gcgcattcgc cgcagcagga agtcgatcgt
     2521 gctttgcctg ggatccgttg acggcgccag gttctcgggt accaagtagt cgtccagggc
     2581 ctcgcgcatc tgcacggccg actcgtagcg gttctccgga tcgcgctcca gcgcccggta
     2641 cacgaggttt gcgagccgct cctcaagcag gttgcgggca tcttgcgaca ggcggatatc
     2701 ttcgctggca atccgcttca tcacctccgc gctgtcggtc ccgggaaccg cccgctgccc
     2761 ggtgagcaat tcgtaaagca ccagccccgc tgaaaagatg tccgatcttg cactgctgct
     2821 tcgccgcagg atgtactctg gagccatgta cgcgggggtc ccgatcagtt gcccgcggtc
     2881 gtcggtcaaa ccttcggtac gcgccgcaat gccgaaatcc attactctcg gcacgccatc
     2941 gtcgtcaatc aggatgttgc tgggcttgag atcccggtga atgatcccgg ccgcatgcgc
//
LOCUS       DAOSIT010000273         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28665_length_2995_cov_7.002041, whole genome shotgun sequence.
ACCESSION   DAOSIT010000273 DAOSIT010000000
VERSION     DAOSIT010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28665_length_2995_cov_7.002041"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLL14_07310"
     CDS             complement(<1..282)
                     /locus_tag="PLL14_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG03773.1"
                     /translation="MAGMIRQLGWFVFVGCAAAATHWLIAVACVAVWHLPPLAANVCG
                     WLVAFLVSFSGHYLLTFRQQRKSLAVACRRFFLVSAAGFMINEVSYACLL"
     gene            281..1162
                     /locus_tag="PLL14_07315"
     CDS             281..1162
                     /locus_tag="PLL14_07315"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOG03774.1"
                     /translation="MQQTCSKASTRSPNQARKGIVICADDFGMSAGIDAGILQLASAG
                     RLSAVSCLTQGSTWKHQTRSLAALPVDIGLHLNFTESFASGQFFLPLPRLIAACYGRR
                     LPAAAIAAAINAQLDAFEAGFGRPPDYIDGHQHVHQLPMLRESLLQIMALRYPGQGLW
                     LRSTRPPSRGNPYPLKAAIISMLGARKMRRLVSAQGLPMNGRLLGVYDFSASREKYAD
                     LLRTWLAIAADGDLLMCHPAVFAEPADGIGPQRTREFSVLSDSRFPTWLEQQGLTLSR
                     LSLETVRPTGTPGRHPG"
     gene            complement(1185..>1579)
                     /locus_tag="PLL14_07320"
     CDS             complement(1185..>1579)
                     /locus_tag="PLL14_07320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG03775.1"
                     /translation="GHIHFSPLLQRTPTATEALYLLFKHAFELGYRRCEWKCDALNAP
                     SRAAAQRLGLVFEGIFRQATVVKGRNRDTAWYAVIDREWPALDAAFSRWLDPANFDAG
                     GEQRVALSSLTRAALAAEAGLCPPARTI"
     assembly_gap    1580..1679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1767..2972)
                     /locus_tag="PLL14_07325"
     CDS             complement(1767..2972)
                     /locus_tag="PLL14_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03776.1"
                     /translation="MFRTLNQPSLWAITLAAAGILMVTMGARQSLGLFLSPLNTATGL
                     GVASISLAMAVGQFTWGAIQPLAGAAADRFGPARVLLGGLLMLALGSALTPFMSSTWG
                     LIVSLGLLSAIGSGAGSFSVLIGASSQRLPITARGAASGVINAGGSFGQFVFAPILQA
                     LIQVLGWMGAMWSLALMTLAALPLVRVVSKPVENPPAPSAGETGLWRSVRGAMADGSY
                     QLLNLGFFTCGFHIAFLVTHLPGEVDLCGLPTTVASWALAIIGLFNIAGSLYAGSCVA
                     RYRSKYVLFWMYGSRALMVGLYLLAPKTELTFYVFAAGLGFTWLATVPPTAAIVGKLF
                     GVRYLGTLFGMSLLSHQIGGFLGAYLGGIALTQFGDYAWMWYADIALAAAAALLNLPI
                     REAALPATA"
BASE COUNT          586 a          986 c          912 g          411 t
ORIGIN      
        1 caacaggcag gcgtaggaca cttcgttgat catgaagccg gccgctgaaa ccagaaagaa
       61 acgccggcag gccacggcca gggatttgcg ctgctgacga aaagtcagca gatagtggcc
      121 gctgaaagac accaggaagg cgaccagcca cccgcagaca ttggccgcca gcggcggcag
      181 atgccagacc gcaacgcacg cgaccgcaat cagccagtgt gtggcggcgg cggcacaacc
      241 gacgaagaca aaccaaccga gctgccggat cattcctgcc atgcaacaaa cctgtagtaa
      301 ggcgtctacg cgatctccga accaagcccg gaaagggatc gtcatctgcg ccgatgattt
      361 cgggatgagc gccggcatcg acgccggcat cctccagttg gccagtgccg gccgcctcag
      421 cgccgtcagt tgcctgaccc agggatcgac atggaaacac cagacacggt cgttggcggc
      481 gctgccggtg gacatcggac tgcacctcaa ttttacggag tccttcgcga gcgggcagtt
      541 tttcctgccg ctaccgcgcc tgattgccgc ctgttacggg cgacgcctgc ctgccgcagc
      601 gatcgccgcg gcaatcaacg cccagcttga tgccttcgag gccggctttg gacgcccgcc
      661 ggattacatt gacggccacc agcacgttca ccaattgccg atgctgcgcg aaagtctgct
      721 gcagatcatg gccctgcgct accccgggca aggcctctgg ctgcgctcga ccaggccgcc
      781 atcgcgtggg aacccgtacc cgctgaaggc cgccatcatc tcgatgctcg gagcacggaa
      841 aatgcgccgt ctggtcagcg cgcaagggtt gccgatgaac ggtcgcctgc tgggcgtcta
      901 tgacttttcc gcaagccggg agaaatacgc ggacctgctc aggacctggc tggccatcgc
      961 cgctgacggc gacctgctga tgtgccaccc ggcggttttc gccgaacccg cagacggcat
     1021 cggaccgcaa cgcacacggg aattctcggt gctctccgat agccgcttcc ccacctggct
     1081 ggaacagcag ggcttgaccc tgtcgcggct gtcgctggaa accgtccgcc cgacaggcac
     1141 gcccgggcgg catccggggt gagaagtggc ggccgctcgc ctcctcagat cgtccgcgcc
     1201 ggcgggcaaa gacccgcttc tgcggcgagc gccgcccgcg tcagcgacga gagcgcgacg
     1261 cgctgctcgc cgccggcgtc gaaattggcc gggtcaagcc agcgcgagaa agcggcgtca
     1321 agggctggcc attcgcggtc gatcaccgcg taccaggcgg tatcccgatt gcggcccttg
     1381 accaccgtcg cttgtcggaa gatgccttca aataccaggc caaggcgctg cgctgccgcc
     1441 cgcgaaggcg cattgagggc atcgcatttc cactcgcagc gtcggtaacc cagttcaaaa
     1501 gcgtgtttga acaatagata cagggcttcg gtggctgtcg gcgtgcgctg cagaagcggc
     1561 gagaaatgga tatgccccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     1681 ccccgctggc ggtggcggcg ccctccagcc gggcagcggc tccccgacgg gttgctcgtc
     1741 agcgctgccg gtgcgcagac cccggctcat gccgttgccg gcagcgccgc ctccctgatc
     1801 ggcagattca gcagcgccgc tgccgccgcc agcgcgatgt cggcgtacca catccaggcg
     1861 tagtcgccga actgcgtcag tgcgatgccg cccaggtagg ctccgaggaa accgccgatc
     1921 tggtgcgaga gcagtgacat gccgaacaag gtgccgagat agcgcacgcc gaagagtttg
     1981 ccgacgatcg ccgcggtcgg cggcacggtc gccagccagg tgaaaccaag accggccgcg
     2041 aaaacgtaga aggtcagttc ggtcttcggt gcgagcagat acagcccgac catcagcgcg
     2101 cgtgacccgt acatccagaa caggacatac ttgctgcgat agcgcgcgac gcacgaaccc
     2161 gcatagaggc tgccggcgat attgaacagc ccgatgatcg ccaacgccca actggccacc
     2221 gtcgtcggca gaccacaaag gtccacctcg cccggcagat gcgtcaccag aaaggcgatg
     2281 tgaaaaccac aggtgaagaa gccgagattc aggagctgat agctcccgtc cgccatcgcg
     2341 ccccgcacgc tccgccacag gcccgtttca ccagcagaag gggcgggtgg attttcgacc
     2401 ggcttcgaga ccacccgcac caggggcaag gccgccaggg tcatcaacgc cagcgaccac
     2461 atcgccccca tccagccgag cacctgaatc agcgcctgca ggatcggcgc gaagacgaac
     2521 tggccgaacg agccaccggc gttgatcacc cccgaggcgg cgccgcgggc ggtgatcggc
     2581 aggcgttggc tggaagcccc gatcagaacc gaaaagctcc ctgcccccga accgatcgcc
     2641 gaaagcaggc cgagcgagac gatcagtccc caggtgctgc tcatgaacgg cgtcagcgca
     2701 ctaccgaggg ccagcatcag gagcccgccc agcaacactc gcgccggccc gaagcgatca
     2761 gcggcagccc cggcgagagg ctgaatggcg ccccaggtga attgcccgac ggccatcgcc
     2821 aggctgatcg acgccacgcc aagaccggtg gccgtattca gcggcgaaag aaagagcccg
     2881 agcgattgcc gcgcccccat ggtgaccatc aggataccgg ccgccgccag ggtgatcgcc
     2941 cacagcgacg gttgattgag cgttcgaaac atggcggcga ccttggtcaa caatg
//
LOCUS       DAOSIT010000274         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28730_length_2990_cov_5.605111, whole genome shotgun sequence.
ACCESSION   DAOSIT010000274 DAOSIT010000000
VERSION     DAOSIT010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28730_length_2990_cov_5.605111"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2990
                     /locus_tag="PLL14_07330"
     CDS             <1..>2990
                     /locus_tag="PLL14_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03777.1"
                     /translation="DAENRARREYEVMQHWQKSPFLPSLLDSFQEAERYPGELYWLSL
                     VDPAAPTLTERAKDASWSLDDRLRYAREALLALAEFHQPADQTQHRILHRHITPLTLR
                     VRHSGRPLFTDFSLARLDQALTISRAPMDFGEDAPYVAPEVHEGGLAAADARSDVFAL
                     CASLITLFPAEESRAREAREFLEQGCALNPEGRESLAELASVLERNTAPLARPTLELP
                     APEYWDEDTVVPFQRAHFKIISRLGSGGIGQTFKVVEVDARSDEVYGSYVAKLIHHQA
                     DGEVALRAYRRARAYTIHPHLSAIHEIAPEWQAHRFVALMKWVEGMPLSELTGVLALH
                     AEELGETSQQDLLLRWLRDLCDALWALHQVGLVHGDVSPRNIIVQGGEVVLTDYDTVS
                     EAGTAVRSRNPLYASHSVESGAAIQTGDDLFALAASFFHVLFDKDPFDFAGQRLKNRG
                     LNWQGVDATGLERVVEFMKRATTPIEGERFEDARAAFSFLSAASTDGEVIGVPLAPAL
                     SANSAPRLAELLSAYPGSRHGNSETRGLDSAFATATYVETRLDDVLRQEIEDDRVKLA
                     ILFGNAGDGKTAFLQHLLAALGVRDVHSSQRVQERRLADGRRLKVNLDGSAAWQGQSA
                     NALLDQFFQPCHELVFEGAARHPRVLAINSGKLLEWLETREDTPLSEQLYAALFESED
                     DDELAIDPRIRLIDLNRRSLVGGIANGELRADFLSALLDRFLGVGQEADPWAGCATCT
                     AQHRCTAWHSVRTLRDADTGPHLRARLADALQACHLRGEIHITARELRAALAFLLFGV
                     HDCGELHADPELMPPRYWDRAFAADAPQRQGELLAELARFDPALDSNPLLDRHLLRHT
                     PHGPNDIAAALASARRRAWFEWDEARYAALQLPTDALPLFGGRHLDRFRRVPLMNEDE
                     RADLCRELCLGIARLEDLPEAAFSSESGLPLRIQPRTPTESAFWVVKPWKRFHLEARL
                     PHTAEGLEALHTHLLLIYGYGIGG"
BASE COUNT          505 a          995 c          931 g          559 t
ORIGIN      
        1 aggatgccga gaaccgcgcc cggcgcgagt acgaagtcat gcagcactgg cagaaatcac
       61 cctttctgcc gagtttgctc gactcgtttc aggaggccga acgctatcca ggcgagttgt
      121 actggctctc cctggtcgat cccgccgcgc caacgctcac cgaacgtgcg aaggacgcaa
      181 gttggagtct ggacgaccgc ttgcgctacg cccgtgaagc gctcctggcc ctggccgagt
      241 ttcatcagcc tgccgaccag acgcaacatc gtatcctcca tcgccatatc acgccgctta
      301 ccttgcgggt gcgtcatagc ggccgcccct tgtttacgga tttcagtctg gctcgccttg
      361 accaggcgct gaccatttcg cgtgctccga tggatttcgg cgaggacgcc ccttatgtcg
      421 ccccggaggt tcacgaaggg gggttggcgg cggcggatgc ccggtccgac gtgttcgcct
      481 tgtgcgccag cctgatcacg cttttcccgg ctgaagagtc ccgtgcccgc gaagcgcgtg
      541 agttcctgga gcagggctgc gccctgaatc ctgagggacg cgagtcgctt gccgaactgg
      601 cctctgtgct ggagcggaac accgcgccac ttgccaggcc gacactcgaa cttcctgcgc
      661 cggaatactg ggacgaagac accgtggtgc ctttccagcg cgcgcacttc aagatcatca
      721 gccgtcttgg gagtggcggc atcggccaga cgttcaaggt cgtcgaggtc gatgcgcgct
      781 ccgatgaagt ctacggcagc tacgtcgcca agctgatcca ccaccaggcc gatggcgaag
      841 tcgccttgcg cgcctatcgc agggcgcgcg cctacaccat tcacccacac ctttccgcca
      901 tccacgaaat cgccccagag tggcaggcgc accgcttcgt cgccttgatg aaatgggtcg
      961 aagggatgcc gttgtccgaa ctcaccggcg tgctcgcctt gcacgccgag gaattggggg
     1021 aaaccagtca gcaagatctg ctgctgcgct ggttgcgcga cctgtgcgat gcgctttggg
     1081 ccttgcatca ggtgggtctg gtgcatggcg atgtcagccc gcgcaacatc atcgttcagg
     1141 ggggcgaagt ggtgctcacc gattacgaca ccgtcagcga agccggcacc gcggtgcgca
     1201 gccgcaatcc cttgtatgcc agccacagtg tcgagagtgg tgcggcgatc cagacggggg
     1261 atgatctctt tgccttggcc gccagttttt tccacgtgct gttcgacaaa gaccccttcg
     1321 actttgccgg acaacgcctc aagaaccgcg ggctgaattg gcagggcgtg gacgccacgg
     1381 ggcttgaacg ggtcgtcgag ttcatgaagc gcgcgacgac gcccatcgaa ggcgaacgct
     1441 tcgaagatgc ccgcgcagcg ttctctttcc tctccgcggc aagtaccgat ggcgaagtga
     1501 ttggcgtacc gttggcgccg gcgctctccg ccaacagcgc accgcgcctg gccgaactgc
     1561 tctccgccta tccgggctcc cgccacggca acagcgaaac gcgggggctc gattcggctt
     1621 tcgccaccgc cacctacgtc gagacacggc tggatgatgt tctgcggcag gaaatcgaag
     1681 acgatcgggt caaactcgcg atcctgtttg gcaatgccgg tgacggcaag accgcgttcc
     1741 tgcagcactt gctggcggcc ctgggggtgc gcgatgtcca ctcttcacag cgcgtgcagg
     1801 agcgccgcct ggccgatggg cgcaggttga aggtcaatct cgatggctcg gcggcgtggc
     1861 agggccaatc ggccaatgcg ctgctcgacc agttcttcca gccctgccat gaactggttt
     1921 ttgaaggtgc ggcacgccat ccccgcgtgc tcgccatcaa cagcggcaag ctgctggaat
     1981 ggctggagac gcgggaagac acgccgctga gcgaacagct ctatgctgcc ctgttcgagt
     2041 ccgaagacga cgatgaactg gcgatcgacc cgcgcattcg cctgatcgat ctcaaccggc
     2101 gctcgctggt gggaggaatt gccaacggcg agcttcgcgc cgatttcctc agtgcgctgc
     2161 tggaccgttt tctcggcgtt ggccaggaag ccgacccctg ggcgggctgc gcgacctgca
     2221 cggcgcaaca tcgttgcacc gcctggcatt cagtgcggac cttgcgcgac gcagatactg
     2281 gtccgcacct gcgcgcccgc ctggcagacg ccctgcaagc ctgccacctg cgaggcgaaa
     2341 tccacatcac cgcgcgcgaa ctgcgggcag cgctggcctt cctcctcttt ggcgtgcatg
     2401 actgcggcga actccatgcc gacccagaat tgatgccacc tcgctattgg gatcgagcct
     2461 tcgccgccga cgcgccgcaa cggcagggcg agttgctcgc ggaactcgcc cgtttcgacc
     2521 cggcgctgga cagcaacccg ttgctcgacc gacacctgct caggcacact ccgcacggtc
     2581 cgaacgacat cgccgccgcg ctggcctctg cccgtcgccg cgcgtggttc gaatgggatg
     2641 aggcgcgtta cgccgcgcta caacttccca ccgatgccct gccgttgttc ggcggtcgac
     2701 atctggatcg tttccgccgc gtgccgctga tgaacgagga cgagcgcgcc gacctctgcc
     2761 gggagttgtg tctgggcatc gcccgactgg aggacctgcc cgaggccgcc ttttcctccg
     2821 agtctggcct gccgctgcgc attcagccgc gcacgccgac cgaaagcgcc ttctgggtgg
     2881 tcaagccctg gaagcgtttc cacctggaag cgcgcctgcc gcataccgcc gaagggctgg
     2941 aagccttgca tacccatctg ctgctgattt atggctatgg aattggcggt
//
LOCUS       DAOSIT010000275         2989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_28740_length_2989_cov_7.529652, whole genome shotgun sequence.
ACCESSION   DAOSIT010000275 DAOSIT010000000
VERSION     DAOSIT010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28740_length_2989_cov_7.529652"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLL14_07335"
     CDS             complement(<1..710)
                     /locus_tag="PLL14_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG03778.1"
                     /translation="MARRRVVITGLGIVSPVGNSVAEAWQNILAGRSGIGPVTRFDAS
                     AFPVRIAGEVKGFDVGQYLSPKEARRMDVFIHYGMAAGLQAIRDAGFDANQVRPERIG
                     VSIGSGIGGLPMIESTCDDFAAGGVRKVSPFFVPGSIINMISGNLSIMCGYKGPNISL
                     VSACSTGTHSIGDAGRLIEYGDADIMIAGGAEGCVSNLGLGGFCAPRALSTRNDDPQT
                     ASRPWDRDRDGFVLGEGAG"
     gene            complement(824..1060)
                     /gene="acpP"
                     /locus_tag="PLL14_07340"
     CDS             complement(824..1060)
                     /gene="acpP"
                     /locus_tag="PLL14_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG03779.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADIKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDDEAEKITTVQQAIDYVTAHKK"
     gene            complement(1220..1960)
                     /gene="fabG"
                     /locus_tag="PLL14_07345"
     CDS             complement(1220..1960)
                     /gene="fabG"
                     /locus_tag="PLL14_07345"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOG03780.1"
                     /translation="MLDGQVALVTGASRGIGRAIALELGRLGASVVGTATSPAGADAI
                     SAFLGEAGCRGEGAVLEVRDALQIDALIGHIEKTYGAVTVLVNNAGITRDNLALRLKD
                     DEWETVLDTDLKAVFRLSKAVMRGMMKARQGRIINVTSVVGHAGNPGQVNYCAAKAGV
                     TGMSRALARELGSRNITVNCVAPGLIDTDMTRALDDRQKESLLAGIPLARLGSPPEVA
                     AAVAFLASAGAAYVTGTTIHVNGGMFMG"
     gene            complement(1967..2908)
                     /gene="fabD"
                     /locus_tag="PLL14_07350"
     CDS             complement(1967..2908)
                     /gene="fabD"
                     /locus_tag="PLL14_07350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOG03781.1"
                     /translation="MAFAFVFPGQGSQSVGMMAAYGESRVVRATFDEASAVLDQDLWQ
                     LLAEGPAEALAQTVNTQPVMLVADIAVYRLWLDKGGGLPAVVAGHSLGEYAALVAAGV
                     IAFADAVPLVRLRAAAMQEAVPVGAGAMAAILGLDDEKIAAACAEAEMSAGAGAVVQA
                     VNFNGPGQTVIAGSKAAVELACAGCKARGAKRALLLPVSAPFHSSLLRPAAERLAAGL
                     AGVNFASARIPVINNVDVLIESEPDRIKDALIRQAYSPVRWVETIRKMAAMGITTVAE
                     CGPGKVLAGLSKRCADNLASVALADLVALEAAVANLE"
BASE COUNT          600 a         1046 c          831 g          512 t
ORIGIN      
        1 ccggcccctt cgccgagcac aaagccatcg cgatccctgt cccacggacg gcttgccgtc
       61 tgcggatcgt cgttgcgcgt cgataacgca cgcggcgcac agaagccccc cagccccagg
      121 ttcgagacac agccttccgc gccgccggca atcatgatgt ccgcatcgcc atattcgatc
      181 aggcgcccgg catcaccaat gctgtgggta cccgtcgaac acgcgctgac cagcgaaata
      241 ttgggaccct tgtagccgca catgatcgac agattgcccg agatcatgtt gatgatcgaa
      301 ccagggacga aaaatggcga caccttgcgt acgccacccg cggcgaaatc gtcgcaggtg
      361 ctttcgatca tcggcaggcc accaatcccc gaaccgatgg acaccccgat ccgctcaggc
      421 cgcacctgat tggcgtcgaa tcccgcgtca cgtatggcct gaagtccggc cgccatgccg
      481 taatggatga aaacgtccat ccgccgggct tctttcggcg acagatactg gccgacgtca
      541 aaacccttca cttcaccggc gatgcggacg ggaaaggccg aagcatcgaa acgggtcacc
      601 gggccaatcc ccgagcgccc ggcaagaata ttctgccaag cttcagcgac agaattaccg
      661 actggagaaa cgataccgag gccggtgatg accactctgc gacgtgccaa cttgaactcc
      721 gaacactaga acatgcggaa gccggaactt ccctttttca tggtcacccc ctgcaggaca
      781 cggccagcga cccgtccaca gggacccccc gacgcacggt tggttacttc ttgtgagcag
      841 tcacgtagtc tatggcctgc tgaacagtgg tgatcttctc ggcttcgtca tccgggatct
      901 cgcactcgaa ctcttcttcg agagccatca ccagttccac cgtgtcaagc gaatcggccc
      961 ccagatcatc gacaaacgac gattcattct tgatgtccgc ctcattgaca ccaagttgtt
     1021 ccgcgacgat cttcttgaca cgctgctcga tattttccat caaagtgttc cttccaaagt
     1081 tcaaatgtaa aacaaaacgc gccattctaa caaaatggct gcaacttacc aatcatcctg
     1141 gaaacgctgc tccagcccag gttgacttgc cggaataccc catgaccata tgacccacca
     1201 gggcgagtgc ccggactgct caacccatga acatgccgcc gttgacgtgt atcgtcgtgc
     1261 cggtcacata agcggcgccg gccgaagcga gaaacgcgac ggcggcggcg acctccggag
     1321 gcgatccgag ccgcgccagc ggaatgccgg cgagcaggga ctccttctgc cggtcgtcaa
     1381 gcgcccgcgt catgtcggta tcgatcagac cgggcgcgac gcaattcacg gtgatgttac
     1441 gactgcccaa ctcgcgagcc agcgcgcggc tcatgccggt gaccccggcc ttggcggcgc
     1501 agtaattgac ctggcccgga ttgccggcgt gaccaaccac cgaagtcacg ttgatgatcc
     1561 ggccctgccg ggctttcatc atcccgcgca tgaccgcctt ggaaagtcgg aagacggcct
     1621 tcaagtcggt gtcgaggacc gtctcccact cgtcgtcctt cagacgcaga gcaaggttgt
     1681 cgcgggtaat gccggcgtta ttgaccagga ccgtcaccgc accgtaggtc ttctcgatgt
     1741 ggccgatcag cgcatcgatc tgcagcgcat cgcgcacttc cagcactgct ccctcacccc
     1801 ggcaacctgc ctcacccaga aacgccgaga tcgcgtccgc acccgcgggg ctggttgccg
     1861 tcccgaccac gctggcccca aggcggccga gttccagggc aatcgcccgg ccgatgccac
     1921 gcgacgcacc ggtcacgagg gcaacttgtc cgtcgagcat actcacctac tccaggtttg
     1981 caacggcggc ttccagggcc accagatcag ccagcgcgac actggccaga ttgtcggcac
     2041 agcgtttgct gagaccggcg aggaccttgc cgggaccgca ttcggccacc gtcgtgatgc
     2101 ccatcgccgc cattttccgg atcgtctcga cccaacgcac cggcgagtac gcctgccgga
     2161 tcagcgcgtc cttgatcctg tcgggctccg actcgatcag cacatcgacg ttattgatca
     2221 ccggaatgcg tgcgcttgcg aagttcacgc cggccagccc ggcggccagt cgctccgctg
     2281 ccggccgcag caacgaggaa tggaaaggcg cagaaaccgg cagcaatagc gcgcgcttgg
     2341 caccacgcgc cttgcaccct gcgcaggcga gttcgactgc cgccttgctg ccggcgatga
     2401 cggtctgccc ggggccattg aaattgaccg cttggactac ggcgccggca cctgccgaca
     2461 tttcggcttc ggcgcaggcg gcggcaatct tttcgtcgtc gagccccaga atggccgcca
     2521 tcgcgccagc tcccacgggc accgcttcct gcatcgcggc cgcacgcaga cgcaccaggg
     2581 gcaccgcatc ggcaaacgcg atcaccccgg cggcgaccag tgccgcgtat tcgcccaggc
     2641 tgtgtccggc cacgaccgcc ggcaagccgc cacccttgtc cagccacagc cgatagaccg
     2701 cgatatcggc gaccagcatc acgggctggg tgttcaccgt ctgcgccagc gcttcggcgg
     2761 gtccctcggc aagtagctgc cagaggtcct ggtcgagtac cgccgacgcc tcgtcgaaag
     2821 tggcgcgaac gacgcgtgac tcgccatagg ccgccatcat cccgaccgac tgcgacccct
     2881 gacctgggaa aacgaacgcg aacgccatgt acctaatcct ccttcagagc tcgagcagca
     2941 ccgcgcccca ggtaaaacca ccgccaacgc cttcgagcag gactttctg
//
LOCUS       DAOSIT010000276         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29012_length_2965_cov_9.677663, whole genome shotgun sequence.
ACCESSION   DAOSIT010000276 DAOSIT010000000
VERSION     DAOSIT010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29012_length_2965_cov_9.677663"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..528)
                     /locus_tag="PLL14_07355"
     CDS             complement(82..528)
                     /locus_tag="PLL14_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG03782.1"
                     /translation="MVQRPLFWFLSLLLALPVRAEVVDIDNAELARLAGAGVPVIDIR
                     TAGEWKQTGILPGSRLLTFFDEHGRADPPAWLQQVQAVAGPDQPVIVICRSGNRTRVA
                     SQFLSQQAGYQKVYNVKEGIRSWVGEGRMLTPMPGALATCPSGARC"
     gene            819..2666
                     /locus_tag="PLL14_07360"
     CDS             819..2666
                     /locus_tag="PLL14_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG03783.1"
                     /translation="MKPTDIGAPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYSEAY
                     MVNWVSNVFPGILGRTCDRPCEPACRRSRVEEETADGLNRREPVAICRLKRVAADYKQ
                     DIRGLLPQAASQKNGRRIACVGAGPASLTVARDLAPLGYEVVIYDGDARAGGMIRTQI
                     PKFRLPEEIIDEETGYILGLGVDFRSRQYVSSLKNLLAEGYDAIFVGSGAPRGHDLDI
                     PGRKEAAKYIHLGIDWLASVAFGHVTSISKRVIVLGGGNTAMDCCRSSRRLGGQDVKV
                     IVRSGFEQMKASPWEKEDAMHEGIPILNNLVPKAFTHNGGRLTGVTFQKIRAVFDEQG
                     RRSLVPTGEPDAHFECDEVLVAVGQDNAFPWIERDIGIEFDKWGLPRLDERTMQSTLP
                     NVFFGGDAAFGPKNIIWAVAHGHEAAVSIDRLLNGEDITIRPAPMTNLVSQKMGIHAW
                     RYDNAISVDRRLRVPLRDISAALKSIKIEVELGFDPQLAFKEAQRCLNCDVQTVFTSR
                     LCIECDACMDICPMDCISFTANGEEQELRPRLLAPALNTSQDLYVSETLKTGRVMVKD
                     EDVCLHCGLCAERCPTGAWDMQKFLLVMTHAGSGCRSAGVQGPQPVVVA"
     gene            2676..>2965
                     /locus_tag="PLL14_07365"
     CDS             2676..>2965
                     /locus_tag="PLL14_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG03784.1"
                     /translation="MQKIEAVNDFVVKFANINGSGSASANELFARAILRMGVPVSPRN
                     IFPSNIQGLPTWFEVRVTEHGYLGRRGGVDMMVAMNPQTWDQDVREIDPGGYL"
BASE COUNT          568 a          971 c          920 g          506 t
ORIGIN      
        1 gccgcggtgc cagccagcga tcccaggcct cccattccgc ctgccggtga gcgatatatc
       61 ggcgttcctt gaccatgcag gtcagcaacg tgctccagac ggacaggtgg ccagggcgcc
      121 gggcatcggc gtcagcatcc gaccttcgcc aacccaggag cgtatgccct ccttgacgtt
      181 gtacaccttc tgataacccg cctgctgcga gagaaactga ctcgccaccc gggtccgatt
      241 gccgctgcgg caaatgacga tgaccggctg atcgggcccg gccaccgcct gtacctgttg
      301 cagccaggcc ggcggatcgg cacggccatg ctcgtcgaaa aaggtcaaca gcctgctgcc
      361 cggcaggatt ccggtttgct tccattcgcc ggcggtacgg atgtcgatga ccggcacgcc
      421 cgcgccggca aggcgtgcca gttcggcatt gtcgatatcc accacttcgg cgcggaccgg
      481 aagcgcaaga agcagcgaca gaaaccagaa aagcgggcgc tggaccatga tcgacactcc
      541 tgagacactc gagccgccat catacagtgt ccctctgcca gcgcggcagg gcatgcgcat
      601 cccggcagca ccgacgctga cattccagtg cgcggctgcc aatgcgacga gcaggaaagt
      661 catgaaaatg gcaccggagc ccgcttcccc ggtgcgccct gccctttgcg tcgcgaaacc
      721 cttttcagcg ctgcgggggc gcgttagtat tcatgttcgc tcttttgggg ccagaggcca
      781 tgactctggg ctcgtgccaa ggaaaggata ggttcgtctt gaaacccacc gatatcggcg
      841 cacccgacta cttccacaaa gtcgtggatt gccaatgggc atgcccagcc cacacccccg
      901 ttcccgaata catccgcctg attgccgccg gccgctacag cgaggcgtac atggtcaact
      961 gggtgtcgaa cgtcttcccc ggcattcttg gacgcacctg cgaccgtccg tgcgaaccgg
     1021 cctgtcgtcg cagccgcgtc gaagaggaaa cggcggacgg cctcaaccgc cgggaaccag
     1081 tcgccatctg tcggctgaag cgcgtcgccg ccgactacaa gcaggatatc cgtggcctgc
     1141 tgccgcaagc ggcaagccag aagaacggcc ggcgcatcgc ctgcgtcggg gccggtcccg
     1201 cctctctgac cgtggcgcgt gacctcgcgc cgctcggcta cgaggtcgtc atttacgacg
     1261 gcgatgcccg cgccggtggc atgatccgca cgcagatccc gaaattccgg ctgcccgagg
     1321 agatcatcga cgaagaaacg ggctacattc tcggactcgg cgtcgacttc cgcagccgcc
     1381 agtacgtcag ttcgctgaag aacctgctcg ccgaaggcta cgatgcgata ttcgtcggct
     1441 cgggagcgcc gcgtggccat gatctcgaca ttcccggacg caaggaggcg gcgaagtaca
     1501 ttcatctcgg catcgactgg ctggccagcg tcgctttcgg ccacgtgacc tcgatcagca
     1561 agcgggtgat cgtgctcggc ggcggcaata cggcgatgga ctgctgccgc tcttcacgcc
     1621 gcctcggcgg tcaggacgtg aaggtcatcg tgcgctcagg cttcgagcag atgaaggcca
     1681 gcccctggga gaaggaagac gcgatgcacg aaggcatccc gatcctcaac aacctggtgc
     1741 ccaaggcctt cacccacaac ggtggccgtc tcaccggcgt caccttccag aagattcgcg
     1801 cggtattcga cgaacaggga cggcgcagcc tggtgccgac cggcgaaccc gacgcccact
     1861 tcgagtgcga cgaggtgctg gtggcggtcg gtcaggacaa tgccttcccc tggatcgagc
     1921 gcgacatcgg catcgaattc gacaagtggg ggctgcccag gctcgacgaa cggaccatgc
     1981 agtccacgct gccgaatgtt tttttcggcg gcgacgcggc tttcggaccg aagaacatca
     2041 tctgggcggt ggcgcacggc cacgaggcgg cagtgtcgat cgaccgcctg ttgaacggcg
     2101 aagacatcac catccgaccg gcgccaatga ccaacctggt ttcacagaag atgggcatcc
     2161 atgcctggcg ttacgacaat gccatctcgg tggaccgccg cctgcgcgta ccgctgcgtg
     2221 acatcagcgc cgccctgaag agcatcaaga tcgaggtcga actgggcttt gatccgcaac
     2281 tggcgttcaa ggaggcgcag cgctgcctca actgcgacgt acagacggta ttcacgtcca
     2341 ggctgtgtat cgagtgcgac gcctgcatgg acatctgccc gatggactgc atcagtttca
     2401 cggccaacgg cgaggagcag gagttgcgcc cgcgcctgct cgccccggcg ctgaacacca
     2461 gccaggatct ctacgtttcg gagaccctga aaaccggccg ggtgatggtc aaggacgaag
     2521 atgtgtgcct gcactgcggc ctgtgcgccg aacgctgccc gaccggcgcg tgggatatgc
     2581 agaaattcct gctggtgatg acccacgcgg gctccggttg ccgcagtgcc ggcgtccagg
     2641 gtccgcaacc agtagtcgtc gcctgaggag cgaaaatgca gaagatcgaa gcggtcaatg
     2701 atttcgtcgt caagttcgcc aatatcaacg gctcgggctc ggcttcagcc aacgaactgt
     2761 tcgcgcgcgc catcctgcgc atgggtgtgc cggtctcacc gcgcaacatc ttcccgtcca
     2821 acatccaggg gctgccgacc tggttcgaag tgcgcgtcac cgagcacggc tatctcggcc
     2881 ggcgcggtgg cgtggacatg atggtggcga tgaatcccca gacctgggat caggatgtcc
     2941 gggaaatcga ccccggcggc tacct
//
LOCUS       DAOSIT010000277         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29080_length_2960_cov_7.042685, whole genome shotgun sequence.
ACCESSION   DAOSIT010000277 DAOSIT010000000
VERSION     DAOSIT010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29080_length_2960_cov_7.042685"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..335
                     /locus_tag="PLL14_07370"
     CDS             33..335
                     /locus_tag="PLL14_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HOG03785.1"
                     /translation="MNKTITTASLALALGAALTVAASPAFAADSAADKEKCYGVSLKG
                     KNDCKAGAGTSCAGTSKTDYQGNAWTLVPKGTCEKTVSSTSPTGKGQLAEFKEKKA"
     gene            364..1281
                     /locus_tag="PLL14_07375"
     CDS             364..1281
                     /locus_tag="PLL14_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOG03786.1"
                     /translation="MTIPGNRRPSHGDAPALPVTTLPARGGVGLKPEHFREILDTAPD
                     VGFFEVHAENYMVNGGPFHHYLGRIRQRHPISIHGVGLSIGGEEPLDAAHLDRLAALL
                     ARYQPESFSEHLAWASHGGVYLNDLLPAPYHVGTLARVCAHIDHVQERLQRRMLLENP
                     ATYVEFAASTMAETDFIGEVVRRTGCGLLLDVNNVHVASVNHNRDPRAYIRALPLKAV
                     GQIHLAGFARDIDAAGDPLLIDSHDAPVAQVVWDLYAFALDLLGPQPTLIERDGDIPV
                     FPVLLAEAQRADRIMRQQLAATAPAEACA"
     gene            1278..2063
                     /locus_tag="PLL14_07380"
     CDS             1278..2063
                     /locus_tag="PLL14_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOG03787.1"
                     /translation="MSAQRHFAEAVLAADPVCPPGLMAWNGSDPARRFVVYRNNVMVS
                     LIDALADSYPVTQDLVGEEFFRAMARLFIRTHPPRSPVLALYGAGLAEFIEGFAPAAV
                     LPYLADVARLEMLRVQAWHAADAAPLGLADFAALLENAPALAATGFDLHPSLAVLRSA
                     HAVVSLWAAHQAQDGTPDLAAVDPALPESALVLRIGLDVEIRRIPCATAQFILHLQQG
                     LAFGAAAQFALDNEPAFDLPAVLGMLICGGAIINLSQPRREPA"
     gene            2063..2596
                     /locus_tag="PLL14_07385"
     CDS             2063..2596
                     /locus_tag="PLL14_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOG03788.1"
                     /translation="MNSIATVNAASQAGSSGPIGLVLKLIAVFGRIPDSLIALLGRFS
                     IAAIFWKSGQTKVQGLVIDIVSGEFHFGVPQLSDSALSLFREEYRLPLVAPELAAPLA
                     AFAEHLFPLLILLGLATRFSAIALLVMTLAIQFLVYPDAYPTHGVWASVLVFLIARGP
                     GAVSVDHLIAARFKPIS"
     gene            complement(2768..>2960)
                     /locus_tag="PLL14_07390"
     CDS             complement(2768..>2960)
                     /locus_tag="PLL14_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOG03789.1"
                     /translation="TLLAFETHWGEEDRQTQRELPRLNVEEGALYDDLRDNRLRRNLR
                     LEQERIGFGWVEAALADLA"
BASE COUNT          495 a         1022 c          910 g          533 t
ORIGIN      
        1 caagccatcc aacccaaaca aggagtgtac cgatgaacaa gacaataacg acagcttcgc
       61 tggccctggc gctcggcgcc gccctgacgg tagcggccag cccggctttc gccgccgata
      121 gcgcggccga caaggaaaaa tgctacggcg tctccctcaa gggcaagaat gactgcaagg
      181 caggcgccgg cacctcgtgt gccggtacct cgaaaaccga ctaccaaggc aatgcctgga
      241 ccctggtacc caaggggacc tgcgaaaaga ctgtttcctc gacttcgccg accggcaagg
      301 gccagcttgc cgagttcaag gaaaagaagg cctgatcgaa atccggatcg cggagcatcc
      361 gccatgacga tccctggcaa tcgccggcca tcccacggtg atgcccccgc gctccccgtc
      421 acgaccctgc ccgcgcgcgg cggggtcggg ctcaaaccgg aacattttcg cgagatcctt
      481 gacacagcgc cggatgtcgg cttcttcgag gtgcatgcag agaactatat ggtgaacggc
      541 ggacccttcc accactacct gggccgcatt cgccaacgcc atcccatttc gatccatggt
      601 gtcggcctgt cgatcggcgg cgaggagccc ctggacgccg cgcatctgga ccggctggcc
      661 gcgctgctgg cgcgttacca gccggaatcc ttctccgaac acctggcatg ggccagccac
      721 ggcggggtct atctcaacga cctgctgccg gcaccttatc acgtcggcac gctggcgcgg
      781 gtttgcgcgc atatcgacca tgtgcaggag cgtttgcagc ggcggatgct gctggagaac
      841 cccgcgacct acgtcgaatt cgccgcctcg accatggccg aaaccgattt catcggtgaa
      901 gtcgtgcgcc gcaccggctg cggcctgctg ctggacgtca acaacgtcca tgtcgccagc
      961 gtcaatcaca accgcgaccc gcgcgcctat atccgcgccc tgccgctgaa ggcggtgggc
     1021 cagatccatc tggccggctt cgcccgcgac atcgacgccg cgggcgatcc cctgctgatc
     1081 gacagccacg acgcgcctgt ggcgcaggtg gtctgggatc tctatgcatt cgcactggac
     1141 ctgctgggtc cgcagccaac cctgatcgag cgtgacggcg acatacctgt gtttccggtc
     1201 ctgctcgccg aggcgcagcg ggcggatcgg atcatgcgcc agcagttggc cgctaccgcg
     1261 ccggctgagg cttgcgcgtg agcgcgcaac ggcacttcgc cgaggccgtg ctggctgccg
     1321 atccggtatg cccaccgggc ctgatggcct ggaacggctc agacccggcg cggcgtttcg
     1381 tcgtctaccg caacaatgtc atggtctcgc tgatcgacgc actggccgac agctatccgg
     1441 tgacgcagga tctggtcggc gaggaattct tccgcgccat ggcgcgcctg ttcatccgca
     1501 cccatccgcc gcgctcgccg gttctggcgc tctacggcgc gggtctggcg gaattcatcg
     1561 aaggttttgc gccggcggcc gtcctgccct acctggccga cgtcgcccgg cttgagatgc
     1621 tccgcgtgca ggcctggcac gccgccgatg cggcgccgct tggcctcgcg gactttgcgg
     1681 ccctgctgga gaatgcgccg gcactggcgg caactggttt cgacctgcac ccctcgctgg
     1741 cggtgctgcg ttcggcccac gccgtggttt cgctgtgggc tgcgcaccag gcgcaagatg
     1801 gaacgcccga cctggcggcg gtcgatcccg cgctgcccga atccgctctc gtcctgcgga
     1861 tcgggctcga cgtcgaaatt cggcggattc catgcgccac ggcacaattc atcctgcatc
     1921 tgcagcaggg cctggccttc ggcgccgcag cccaatttgc cctggacaac gagcccgcat
     1981 tcgatctgcc cgccgtgctc ggcatgctga tctgcggcgg agccatcatc aacctgagtc
     2041 agccaagaag agaaccggca tgatgaattc catcgccacc gttaacgcag ccagccaggc
     2101 tgggagtagc ggtccaatcg gcctcgtgct caagctgatc gccgtgttcg ggcgcatccc
     2161 cgatagcctg atcgccttgc tcggccgctt ttccatagcg gcgatcttct ggaagtcggg
     2221 acaaaccaag gtacagggcc ttgtcatcga catcgtcagc ggcgagtttc atttcggcgt
     2281 gccgcagctc tcggactctg ccctgagtct gtttcgcgaa gaatatcggc taccgctggt
     2341 agcgccggaa ctcgcggcgc cgctggctgc cttcgccgag cacctgtttc cactgttgat
     2401 cctgctcggc ttggccacac gcttctccgc gatcgctctg ctggtaatga ccctggcgat
     2461 ccagttcctg gtctaccccg acgcctatcc gacccacggc gtctgggcca gcgtactagt
     2521 cttcctgatc gcccgcggac cgggcgcggt gtcggtggat cacctgatcg ccgcacgctt
     2581 caagccgatc tcataagacg gcggcgatag aaggcagtgc gcgcgggcgc tgaggcggca
     2641 catgggaatt cgggccgtat agttaaccgc aaggcaggca gcacggctac tcgggctgga
     2701 cggcgggtcg cgctggcgcg ggtctgagca aacctgagga tcgatcaggc ggacttgcag
     2761 caacctgtca cgccaaatcc gccagcgccg cctcgaccca gccaaagccg atcctttcct
     2821 gctcaagacg gagattcctg cgcaagcggt tgtcgcgcag gtcgtcatac agggcgccct
     2881 cctccacgtt cagccgtggc agttcgcgct gcgtctgcct gtcctcctcg ccccagtgcg
     2941 tctcgaaagc cagcagggtc
//
LOCUS       DAOSIT010000278         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29094_length_2959_cov_11.961433, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000278 DAOSIT010000000
VERSION     DAOSIT010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29094_length_2959_cov_11.961433"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..424
                     /gene="glnK"
                     /locus_tag="PLL14_07395"
     CDS             86..424
                     /gene="glnK"
                     /locus_tag="PLL14_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOG03790.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAISDSLLDQVIEAIEKSASTGKIGDGKIFISAIEQVIRI
                     RTGETGEEAL"
     gene            435..>1327
                     /locus_tag="PLL14_07400"
     CDS             435..>1327
                     /locus_tag="PLL14_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HOG03791.1"
                     /translation="MRRIFAILALLGAVSIGAPAWAEDKPAAAPATTTAVAAPAAADA
                     ASAAPAAAATAPAPVANKGDNAWVMTSAALVILMSIPGLALFYGGLVRTKNMLSVLMQ
                     VFVTFSLISVLWVVYGYSVAFTEGGGFFGVLDKIFLKGITVDSVAATFSKGVVISELA
                     YVVFQGAFAAITCGLIVGAFAERAKFAAILVFMVIWFTLSYLPMAHMVWYWAGPDAYV
                     DAAAGEAAGKTAGFLFQKGALDFAGGTVVHINAAMAGLVGALLIGKRIGYGRESMAPH
                     SLTFTMIGASLLWFGWFGFNAG"
     assembly_gap    1328..1427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1437..>2959)
                     /locus_tag="PLL14_07405"
     CDS             complement(1437..>2959)
                     /locus_tag="PLL14_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03792.1"
                     /translation="GTVLGAVSMKVSLKSTRDAVARQRMQTIVAAVLLTLPMLLVIFV
                     FIRKVVTRPLAHGVDVAHAVAAGKLDNDIEVVSSDEVGDLLKTLKVMQEGLNVVLQEV
                     ESCGLSMGQSSYQISSISNEISKVNQQQQSRSSEVDGAMSQLDEISRQALAQAVTAVE
                     RSNHVEDMARAGIANVESNIEAMNATSARVEQVSNEIRDLQRSAREIDEIANTIKEIA
                     GQTNLLALNAAIEAARAGEQGRGFAVVADEIRKLAERSARSAERVGVIIGHLSGQVGQ
                     VVSTIDALVASVGSTREESRRMAETMERISNNVAETVRANQNISAASTQQLDQFRLLQ
                     QTLDTLFQTLRESSNKVEVTAAISDDLRSVAARLNTIMARFSFTHSTLIDKAQNEQRR
                     APRLQSNLRVLVEQRGERFEGVTSDVSLVGARLRLRRSIASDQALTVLIYLPAEEMAL
                     YEQQAPLKVSGRVAWSEGGSGEDCVCGVEFFELDEQQRAGLARCFEYFGKNPLFNR"
BASE COUNT          527 a          918 c          835 g          579 t
ORIGIN      
        1 cgccgtcgtt tcagtcagcc ggagctttta ggcaaacatt ggttaaaccg gggaggcgcc
       61 gccttcccat gacatggaga gcaacatgaa actggtaacc gccatcatca agcccttcaa
      121 gcttgacgaa gtacgagaag cgctgtcggc gatcggcgta caggggatca ccgtcaccga
      181 ggtcaagggc ttcgggcgcc agaagggcca caccgagttg tatcgcggcg ccgaatatgt
      241 cgtcgacttc ctgcccaagg tcaaggtcga ggctgcgatc agcgacagtc tcctcgatca
      301 ggtgatcgag gccatcgaaa aatctgccag taccggaaag atcggcgacg gcaagatctt
      361 tatctcggca attgaacaag tcatccgcat tcgcaccggc gaaaccggtg aggaagccct
      421 gtaaggagcc catcatgaga cgcatattcg ccatcctcgc cctgctcggc gccgtaagca
      481 tcggcgctcc ggcctgggcc gaagacaaac ctgcagcagc gcctgcaacc acaacggcgg
      541 tggccgcacc agccgccgcc gatgccgcca gcgctgcacc ggccgcagcc gccacggcgc
      601 cggcaccggt tgccaacaag ggggacaatg cctgggtgat gaccagcgct gccctggtca
      661 tcctgatgtc tatccccgga ctggcactgt tctacggagg gctggtccgc accaagaaca
      721 tgctttcggt gctgatgcag gtgttcgtga ccttctcgct gatcagcgtg ctgtgggtgg
      781 tctacggcta ctcggtggcc tttaccgaag gcgggggctt cttcggcgtg ctcgacaaga
      841 tattcctcaa gggcatcacg gtcgactcgg tcgcagccac cttcagcaag ggcgtggtca
      901 tctctgaact ggcatacgtc gtgttccagg gcgccttcgc agcgattacc tgtggtctga
      961 tcgtcggcgc ctttgccgag cgtgcgaagt ttgccgcgat tctcgtcttc atggtgatct
     1021 ggtttaccct ttcgtacctg ccaatggcgc acatggtctg gtactgggcc ggtcctgacg
     1081 cctatgtcga cgctgcggct ggcgaagcgg caggcaagac ggccggcttc ctgttccaga
     1141 agggcgctct cgactttgcc ggcggaaccg tggtgcacat caacgcggcc atggccggtc
     1201 tggttggagc gctgttgatc ggcaagcgga ttggttatgg ccgtgagtcg atggctccgc
     1261 acagcctgac cttcacgatg atcggtgctt cgctgctgtg gttcggctgg ttcggtttca
     1321 acgccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg ccagccttat
     1441 cggttgaaca aggggttctt gccaaaatac tcaaagcagc gcgccaagcc ggcccgttgt
     1501 tgttcgtcga gctcgaagaa ttcgacaccg caaacacaat cttcgccgct acccccctcg
     1561 ctccacgcca cacgcccgct gaccttgagt ggggcctgct gctcgtatag agccatctcc
     1621 tcagctggca ggtaaatcaa cacggtcaga gcctgatcgg aagcgatgga ccgtcgcagg
     1681 cgcaggcgcg cgccgaccag cgaaacgtcg ctggtaacgc cctcaaacct ctcaccccgc
     1741 tgctcgacaa gcacacgcag gttgctttgc aaccgtggcg cgcgacgctg ctcgttctgc
     1801 gccttgtcga tgagggtgct atgggtaaat gagaacctgg ccatgatcgt gttgagacgc
     1861 gcagcaactg aacgcaggtc gtcactgatc gcggcggtga cctcgacctt gttgctcgac
     1921 tcgcgcaatg tctggaacag cgtatcgagc gtctgctgca acagcctgaa ctgatcgagc
     1981 tgttgtgtgc tggccgcgga aatgttctga ttggctcgca cagtctctgc gacattgttc
     2041 gaaatgcgct ccatggtctc ggccatgcgc cgcgattcct cgcgcgtcga gccgaccgag
     2101 gcgaccaggg catctatggt cgatacgacc tgccccacct gtcccgacaa atgcccgatg
     2161 atgaccccaa cccgctcggc agacctggcg gagcgctcgg caagtttgcg aatttcgtcc
     2221 gccacgaccg caaagccccg cccctgttcg ccggcacggg ccgcctcgat ggccgcgttc
     2281 agcgccagca ggttggtttg tccggcgatt tccttgatcg tgttggcgat ctcatcgatc
     2341 tctcgtgccg accgctgcag gtcccgaatt tcgttcgata cctgctcgac tctggccgaa
     2401 gtcgcattca tcgcctcgat gttgctctcg acgttggcga tccccgcgcg cgccatgtcc
     2461 tccacatggt tcgagcgctc gaccgcggtg acagcctgcg cgagcgcttg ccgcgagatt
     2521 tcgtccaact gcgacatcgc tccgtccacc tcgctggaac ggctttgctg ctgctggttg
     2581 accttcgaaa tctcgttcga aatcgacgaa atctggtagg acgactggcc catgcttaac
     2641 ccgcagctct cgacttcctg cagcacgaca ttcaggccct cctgcatgac cttcaacgtc
     2701 ttgagcaggt cgccgacttc gtcgctcgaa acgacctcga tatcgttgtc gagcttgccg
     2761 gcagccacgg catgcgcgac atccaccccg tgcgcgagcg gccgcgtgac caccttgcgg
     2821 atgaacacga aaatcaccaa cagcatcggc agggtcagca ataccgcggc cacgatggtc
     2881 tgcatgcgct ggcgcgccac cgcatccctg gtcgatttca gcgaaacctt catgcttacc
     2941 gcacccagga cggtgccca
//
LOCUS       DAOSIT010000279         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29192_length_2951_cov_7.701657, whole genome shotgun sequence.
ACCESSION   DAOSIT010000279 DAOSIT010000000
VERSION     DAOSIT010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29192_length_2951_cov_7.701657"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLL14_07410"
     CDS             complement(<1..701)
                     /locus_tag="PLL14_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03793.1"
                     /translation="MPIYVFNAVVGTTLPSFDPALDTLSFDGAYTATSLVLTQSGSDT
                     LVSCEGQGVLLSGVNRSSLGGSQFAFADGSLFRQGTAASDSLTGSSFGDQFDITAGGS
                     DTVTGGDGNDVVLAGAALNAADRIDGGSGFADQLRLAGNYLSRVDLNATTVTGIEQFI
                     FGSGGTVRLRLHDQLFASANAAVTFSAVNQGQTDSLQLDGSLITSAGVDATGGDGADV
                     LTGGAVGDHLVGGVG"
     gene            1219..2070
                     /gene="folD"
                     /locus_tag="PLL14_07415"
     CDS             1219..2070
                     /gene="folD"
                     /locus_tag="PLL14_07415"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOG03794.1"
                     /translation="MTARILDGNALAQTLRADFKKRAEALATRGNRPGLAVILVGEDA
                     ASQVYVRNKVNACVQAGFHSEKLTYAPDVEARVVLAKIAELNADPRIHGILVQLPLPK
                     HFDVDAVLKAISPEKDVDGFHAENVGALMQGHPRFIPCTPYGIMQFLADAGIDLKGKE
                     AVVLGRSNIVGKPMAMLLMQASATVTVCHSQTRDLAFHTRRADVLVAALGKARFVTAD
                     MVKPGAVVIDVGINRLPDGKLCGDVDFGPVSAIASAITPVPGGVGPMTITMLLANTLE
                     AAEREAR"
     gene            2070..2567
                     /gene="purE"
                     /locus_tag="PLL14_07420"
     CDS             2070..2567
                     /gene="purE"
                     /locus_tag="PLL14_07420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG03795.1"
                     /translation="MPQRDPLVGVVMGSDSDWPTMQAAAVMLKEFGIPFEARVVSAHR
                     TPDLLFEYAAAAGERGLKAIIAGAGGAAHLPGMLAAKTTLPVLGVPVQSKALSGQDSL
                     LSIVQMPKGIPVATFAIGEAGAANAALFAVAMLATGDPELAQRLGDFRRAQAEKVMAM
                     KLPEV"
     gene            2612..>2951
                     /locus_tag="PLL14_07425"
     CDS             2612..>2951
                     /locus_tag="PLL14_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HOG03796.1"
                     /translation="MILPPATLGMLGGGQLGRFFVAAAHEMGYAVWVLDPDPHSPAAL
                     IADRHLLAAYDDFVALDELAQGCAAISTEFENVPAGTLDYLDKFVVVRPSAAAVSVCQ
                     NRIAEKNFLRD"
BASE COUNT          569 a          938 c          936 g          508 t
ORIGIN      
        1 tcgccgacgc cgccgacaag gtggtcaccg acagcgcccc cggtcagcac gtcggcgcca
       61 tcgccaccgg ttgcgtcgac gccggcgctg gtgatcaaac tgccatcgag ctgcagagaa
      121 tcggtttgtc cctggttgac agcgctgaag gtgaccgctg cgttggccga ggcaaagagt
      181 tggtcgtgga gccgcagccg gaccgtgccg ccggacccga aaatgaactg ttcgataccc
      241 gtgacggtgg tggcattgag gtctacgcgg ctgaggtagt tgccggcaag acgcaactgg
      301 tcggcaaatc ccgacccgcc gtcaatacga tcggcagcgt tgagcgccgc accggcgagc
      361 acgacatcat tgccatcgcc acccgtgacg gtgtcgctac caccagcggt gatatcaaac
      421 tggtcgccaa aggacgatcc ggtcagggag tccgacgctg ctgtgccctg acgaaacagc
      481 gagccatcgg cgaaggcaaa ttggctgcca ccgaggctac tccggttaac cccggagagc
      541 agtacgccct ggccctcgca gctgacgagc gtatccgaac cggactgcgt cagtacgaga
      601 gaagtggcgg tgtatgcgcc atcaaaagaa agcgtgtcaa gcgcagggtc gaatgaaggg
      661 agcgttgtcc caacgacagc attgaagacg taaatgggca ttggaatccc tattgaaaga
      721 gatgaaaggc gccaatggcc gattcggcgt tcagcgcgct gtttagctac atcatgctag
      781 gtcgctacgt cggccccctt cgtcacggcc agggcgggca caaattcctg cccgacgtcg
      841 cctgcggcta cgttgccgcc gtaaagcaac caatctccgg tcgccgtcaa ggacgagcca
      901 atcacctgcc atttcctgga aaatggcaac acgacaatcg ttgaaaacaa cgtgcctgat
      961 catgctgacg tgaggataat agatcattga ttctatgctg aaatgtgtag gtcaacaaca
     1021 aatatttccc cggtctcaag gcacgctttc caaaggaccc aggagcgtgt agtcgccatt
     1081 cccgcggcga gggccaccgc cgagacgcgc tgatttgcgc tagaatcgcg cccttgattg
     1141 atccatgcgc aattcggacg atgcaccgaa cctgggcatg gcacccttct gcttccggcc
     1201 accccaggga aacgcaaaat gacggcaaga atcctcgacg gcaacgcgct ggcgcagacg
     1261 ctgcgcgccg acttcaagaa gcgcgccgaa gcgctggcga cgcgcggcaa ccgccccgga
     1321 ctggcggtga tcctggtcgg cgaagacgcc gcttcgcagg tctatgtgcg caacaaggtc
     1381 aatgcctgtg tgcaggccgg tttccactcc gagaagctga cctacgcgcc ggatgtcgaa
     1441 gcgcgcgtgg tactcgcgaa aatcgccgag ctcaatgccg atccacgcat ccacggcatt
     1501 ctggtgcagt tgccgctgcc gaagcacttc gacgtcgacg cggtgctcaa ggccatttcc
     1561 cccgagaagg acgtcgacgg ttttcacgcc gagaacgtcg gggcgctgat gcagggccac
     1621 ccgcgcttca ttccgtgcac cccttacggg atcatgcagt tccttgccga cgccggaatc
     1681 gatttgaaag gcaaggaagc cgtcgtcctc ggacgctcga acatcgtcgg caaaccgatg
     1741 gcgatgctgc tgatgcaggc cagcgcgacg gtgacggtct gccactcgca gacgcgcgac
     1801 ctggccttcc acacgcggcg tgccgacgtc ctggtggcag cgctcggcaa ggcgcggttc
     1861 gtcaccgccg acatggtcaa accgggcgcg gtcgtcatcg acgtcggcat caaccggctg
     1921 ccggacggaa aactgtgtgg cgacgtcgat ttcgggccgg tcagcgcaat cgcctcggca
     1981 atcacgccgg ttcccggcgg tgtcggcccg atgaccatca ccatgctgct cgccaacacg
     2041 ctcgaagctg ccgaaagaga ggcccgctga tgccgcaacg tgatccgctg gtcggcgtgg
     2101 tcatgggttc cgactccgac tggccgacga tgcaggcggc cgcggtgatg ctcaaggagt
     2161 tcgggatacc gttcgaagcg cgcgtcgtat cggcgcatcg cacgccggat ctgcttttcg
     2221 agtacgctgc ggcggctggc gagcgcggcc tgaaagcgat catcgccggc gccggcggcg
     2281 ccgcacatct gccgggaatg ctggccgcca agaccacgct gccggtgctc ggggtgccgg
     2341 tgcaatcgaa ggcgctgtcc ggtcaggatt cgctgctgtc gatcgtgcag atgccgaaag
     2401 ggatccctgt cgccactttc gccatcggcg aagccggcgc cgcgaatgcc gccctgtttg
     2461 ctgtcgccat gctggccacc ggcgatccgg aactggcgca gcgcctcggc gacttccgtc
     2521 gggcacaggc cgaaaaggtg atggccatga agctgccgga ggtgtgagcg gcctggagga
     2581 gtcgccgacg cgacactcgc gggtcggcca gatgattctt ccgccggcaa ctctcggcat
     2641 gctcggcggc ggccagctgg gccgcttctt cgtcgccgcc gcgcacgaaa tgggttacgc
     2701 ggtctgggta cttgacccgg atccgcacag cccggccgcc ctgatcgccg atcgccattt
     2761 gctggccgcc tatgacgatt ttgtggcgct ggatgaactg gcgcagggct gtgcggcgat
     2821 cagcaccgag ttcgagaacg tccccgcggg cacgctcgac tatctcgaca agttcgttgt
     2881 cgtgcgcccg tcggcagcgg cggtcagcgt ctgccagaat cgcatcgccg aaaagaactt
     2941 cctgcgcgac a
//
LOCUS       DAOSIT010000280         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29392_length_2934_cov_9.862105, whole genome shotgun sequence.
ACCESSION   DAOSIT010000280 DAOSIT010000000
VERSION     DAOSIT010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29392_length_2934_cov_9.862105"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..355)
                     /locus_tag="PLL14_07430"
     CDS             complement(2..355)
                     /locus_tag="PLL14_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOG03797.1"
                     /translation="MQGSFLRFYVNAAQEHHGVLLWEWLLDQGNRHGIRGGSAFRSIG
                     GFGRRHTVHEDRFFELAGSGGIEVEFVVTRDEEQRLLDLVRHEKIRIFYTRIPAHFGV
                     INPDDQDPAGEATED"
     gene            complement(409..795)
                     /gene="crcB"
                     /locus_tag="PLL14_07435"
     CDS             complement(409..795)
                     /gene="crcB"
                     /locus_tag="PLL14_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOG03798.1"
                     /translation="MWKSILAISTGAALGALLRWQLGLKLNSLFPTVPPGTLAANLIG
                     AYVIGLAVAFFASFSALSPEWRLLVITGFCGGLTTFSTFSAELTTLLQEGRISWALAA
                     VAAHVVGSVLMTLAGIATVVWVKASS"
     gene            1106..>1326
                     /locus_tag="PLL14_07440"
     CDS             1106..>1326
                     /locus_tag="PLL14_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG03799.1"
                     /translation="MRRTKIVATVGPATAAAGTMRQLLEAGVDVVRLNAAHADMDTHS
                     NNARQARELAQVLGRSVGVLVDLPGPKIR"
     assembly_gap    1327..1426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1427..2395
                     /gene="pyk"
                     /locus_tag="PLL14_07445"
     CDS             <1427..2395
                     /gene="pyk"
                     /locus_tag="PLL14_07445"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG03800.1"
                     /translation="TLRSRKGMHLPRAEEHVDPFTDRDARALEMAVAAKVDFLGLSFV
                     RRAEDVEQVRALLPRRGMRPALIAKIETASAVDNLAGIINAADGVMVARGDLGIQMPA
                     RRVPLLQKEIIRFCNQAGKPVITATQMLESMTRSPLPTRAEVNDVANAVLDGTDALML
                     SEETAVGSFPGETVRMMSEVAESAEGWPRDRVDPAASVSADADRVAWAVAHAAVQAAE
                     DLGVAAILCPTRSGQTAHRVAAFRPRMPIAAISPSPQVLGGLCLVWGVRPLVVPENLN
                     GEEQIRLAIAAARAAGIVREGDLVALVFGWAGPRAGSTDSVRIVRA"
     gene            2468..>2934
                     /locus_tag="PLL14_07450"
     CDS             2468..>2934
                     /locus_tag="PLL14_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03801.1"
                     /translation="MKILEYTGLDTSRVKASYRKVADAIARHDFRAAQVRKLANLGHG
                     KFYRARLDDSNRLLFSLVRHGDEVCALMLEVIVNHDYDKSRFLRGAAIDDDRIPDIGV
                     EDAIREAQPVRYLHPERTAIHLLDKPISFDDTQDAIYRRPPPLIVVGCAGSGKT"
BASE COUNT          527 a          985 c          885 g          437 t
ORIGIN      
        1 gtcaatcttc ggtcgcctcg ccggcaggat cctgatcatc cggattgatc actccgaagt
       61 gcgccggaat gcgcgtgtag aagatacgga ttttctcgtg acggaccaga tcgagaagcc
      121 gttgctcttc gtcacgtgtc acgacgaatt cgacctcgat gccaccgctt cccgccagtt
      181 caaaaaatcg gtcttcgtgc acggtatgcc gtcgcccgaa accgccgatc gaccgaaagg
      241 ccgacccgcc ccggatgccg tgcctgttgc cctggtcgag cagccactcc cacaacagaa
      301 caccgtgatg ctcctgagcc gcgttcacgt agaatctcag gaatgaacct tgcatgcttg
      361 cctccccgcc ctgtccttgc acgccgctgt cggcaatatg caaccgcatc agcttgacgc
      421 cttcacccag acgacggtgg caatcccggc cagggtcatc agcaccgatc ccacgacgtg
      481 cgccgcaaca gcagccagtg cccaggatat cctcccctcc tgcagcaggg tcgtgagttc
      541 ggccgagaac gtcgagaacg tcgtcagccc gccgcagaag ccggtaatga ccagcagacg
      601 ccattccggc gagagcgctg aaaatgacgc aaagaacgcc acggccaggc caatgacgta
      661 ggcgccgatc agattcgcag ccagggtgcc ggggggcacc gtcggaaaga ggctgttgag
      721 cttgaggccg agttgccagc gcaacaaggc gccaagcgcg gcaccggtcg agatggcaag
      781 gattgatttc cacataccgc taccctgcaa agctggtgcc aaagcggcca ggccgaacgc
      841 atcgacggcc gaacgccatc tcccaagcgc tcaatatagc accggaactt gtgccatgaa
      901 cagcggtcgc gctgcacgcg ggtgccgcca ggcacggccg gtccccgcca taaagcgcag
      961 gcgtggcccg tacctcgatt gacggtgcgt gtctctgctc cctatactcg ggggctttac
     1021 cttggatatc ccgtgttccg ctgcggactt tcgcgcgcat gcgcacccgg caggacagga
     1081 tgtcgaagcg atcgaggagt acgtgatgcg ccgaacgaag atcgtggcca cggtcggccc
     1141 ggccactgcc gcagccggaa cgatgcgaca actgctggaa gccggcgtcg atgtcgtacg
     1201 cctcaacgcg gcgcacgccg acatggacac gcacagcaac aacgcgcgcc aggcgcggga
     1261 actggcgcag gttctcggcc gcagcgtcgg cgtgctggtc gatctgccgg gccccaagat
     1321 ccgcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgc tgcgctcgcg
     1441 caaggggatg cacctgccac gtgccgagga gcacgtcgat ccgttcaccg accgcgacgc
     1501 ccgtgcgctc gagatggcgg tggccgcgaa ggtggacttc ctcggacttt ctttcgtccg
     1561 ccgcgccgaa gacgtcgagc aggtgcgcgc cctgctcccc aggcgcggca tgcggccggc
     1621 actgatcgcc aagatcgaga ccgccagcgc ggtcgacaac ctcgcgggaa tcatcaacgc
     1681 cgccgacggc gtcatggtcg cacgcggcga tctcggcatc cagatgccgg cgcgacgcgt
     1741 gccactcctc cagaaggaga tcatccgctt ctgcaaccag gccggaaaac cggtgatcac
     1801 cgccacgcag atgctggaat cgatgacccg ctcgccgctg ccgacgcgcg ccgaggtcaa
     1861 tgacgtggcc aacgcggtgc tcgacggcac cgacgcactg atgctctcgg aagagacggc
     1921 agtcggatcg ttccccggag aaaccgtgcg catgatgagc gaagtcgccg aatcggccga
     1981 aggctggccg cgcgaccgcg tcgatccggc cgccagcgtc agcgccgacg ccgaccgcgt
     2041 cgcctgggcg gtggcgcacg ccgcggtaca ggcggccgaa gacctcggtg tggcggcgat
     2101 cctgtgtccc acgcgcagcg gtcaaacggc gcatcgcgtc gccgccttcc ggccgcggat
     2161 gccgatcgcc gccatctcgc ccagcccgca ggtgctcggc gggctctgcc tggtgtgggg
     2221 cgtgcgaccg ctggtggtcc ccgaaaacct caacggcgag gaacagatac ggctcgcgat
     2281 cgcggccgca cgggcggcgg gcatcgtccg cgaaggcgat ctggtggcgc tcgttttcgg
     2341 ctgggccggg ccgcgcgccg gcagcaccga cagcgtacgc atcgtgcgcg cctgaggcga
     2401 agcatgtcgg acccagctgc ctacgctgcg cctgcgcctg tacactttca gcgtatcctg
     2461 gccgaccatg aagattcttg aatataccgg ccttgacact tcccgcgtca aggccagcta
     2521 ccgcaaggtg gccgacgcca tcgcccgcca cgacttccgc gccgcgcagg tcaggaagct
     2581 cgccaatctc ggccacggca agttctaccg ggcccggctc gacgacagca accggctgtt
     2641 gttctcgctg gttcgccacg gcgacgaagt ctgtgccctg atgctcgagg tcatcgtcaa
     2701 tcacgattac gacaagtcgc gctttctgcg cggcgccgca atcgacgatg acaggattcc
     2761 cgacatcggc gtcgaggacg ccatccgcga agcccagccg gtacgctacc tgcatcccga
     2821 gcggacggcg attcatctgc tcgacaagcc gatctccttc gacgacacgc aggacgccat
     2881 ctatcgccga ccgccaccgc tgatcgtcgt cggctgcgcc ggcagcggca agac
//
LOCUS       DAOSIT010000281         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29433_length_2930_cov_8.034435, whole genome shotgun sequence.
ACCESSION   DAOSIT010000281 DAOSIT010000000
VERSION     DAOSIT010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29433_length_2930_cov_8.034435"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLL14_07455"
     CDS             <1..477
                     /locus_tag="PLL14_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOG03802.1"
                     /translation="LTYKNEHYFPLTKDLVLMLMGELGYANGLQGQSLPFYKNFYAGG
                     IGSVRGYQTASLGPVDPLYPDTYLGGTSKAVFNAELLMPLPGFDKSVRLGPFFDAGNV
                     FSDNYTIGKEGLAMSVGLTAAWISPLGPLKFSYGQPINEKSNAKLQKFQFQMGTTF"
     gene            487..1011
                     /locus_tag="PLL14_07460"
     CDS             487..1011
                     /locus_tag="PLL14_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOG03803.1"
                     /translation="MSLKKTIARLLVALMASLPVSYASAVDQLKIGYVNTQRLFRDAP
                     AAVRAAKKIEQEFSKRDQDLQRLAKQVQGLQETLEKGGLTMAESERRAKEKDLGELSR
                     EFQRKQREFREDLNLRQNEENAAIIEKANKAIKQLAENEKFDLIVQDVVWVSPKLDIT
                     DKVIKALSEPQAAK"
     gene            1038..>1486
                     /locus_tag="PLL14_07465"
     CDS             1038..>1486
                     /locus_tag="PLL14_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOG03804.1"
                     /translation="MDVSRGLRLGDIVARLGGVLHGDETVFVSQVGSLLSAGAGQIAF
                     LANPKYRDQLHRTQASAVIVPPPFATETSLPRIVDRNPYAYYARVVGLLNPPAQGRPG
                     IHPGAVVRSTVPSSASVGENVVVGEGVCLGENVILHPGCVIGDGVTIG"
     assembly_gap    1487..1586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1587..1835
                     /locus_tag="PLL14_07470"
     CDS             <1587..1835
                     /locus_tag="PLL14_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HOG03805.1"
                     /translation="GILSFKTLGAKPDSSSLFYFVGIDSARFKKTVTAGDQLHLHVSI
                     LRQMRNIWKFKAEARVDGEIVAEAELMCAKSSIVKAPG"
     gene            1844..2614
                     /gene="lpxA"
                     /locus_tag="PLL14_07475"
     CDS             1844..2614
                     /gene="lpxA"
                     /locus_tag="PLL14_07475"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOG03806.1"
                     /translation="MIHQTAIVHAGAQLGENVAVGPYSIIGEHVEIGDNTIVGPHVVI
                     SGRTRIGCENRIFQFCSLGEVPQDKKYAGEPTCLEIGDRNTIREFCSFNLGTALDAGV
                     TRVGDDNWIMAYVHIAHDCQVGNRTVFANNAQLAGHVHIDDWAILGGYTGVHQFCRVG
                     AHTMTAVGTVVLQDIPPFVMAAGNTASPFGINAEGLKRRGFSAEALLALKRAYRTLYK
                     SGLMLDEARAKLAQEVVAHPEIQPLIDFLAVSKRGIIR"
     gene            2611..>2930
                     /locus_tag="PLL14_07480"
     CDS             2611..>2930
                     /locus_tag="PLL14_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOG03807.1"
                     /translation="MTAAGFRIAMVAGEASGDLLASQLIQALRARLPNAVFYGVGGPK
                     MLGMGFEAWHPLEKLAVRGYVEVLRHYREIAAIRRDLKRRFLADPPDVFIGVDAPDFN
                     LALE"
BASE COUNT          564 a          801 c          891 g          574 t
ORIGIN      
        1 ctgacctaca agaacgaaca ctactttccc ctgaccaaag acctggtgct gatgctcatg
       61 ggggaactcg gttacgcgaa tggtctgcaa ggccagagct tgccgttcta caagaatttc
      121 tacgctggcg gcatcggctc ggtgcgcggc taccagactg ccagtctcgg cccggtagac
      181 ccgctgtatc ccgataccta cctcggaggt acgagcaagg cggtgttcaa cgccgagttg
      241 ctgatgccgc tgcccgggtt cgacaagtcg gtccgtctgg ggccattttt tgatgcgggt
      301 aacgtgttca gcgataacta cacgatcggg aaggaaggac tggcgatgtc cgtcggtctc
      361 accgcagcct ggatatcgcc gttgggaccc ctgaaattca gttatggcca gccgatcaac
      421 gagaaaagta atgctaaact gcagaagttc cagttccaga tggggaccac cttttaatcc
      481 aggagaatga gtttgaagaa gacgattgcc aggctgctgg ttgcgttgat ggcctcgctg
      541 ccggtgtctt acgcgtcggc ggtggaccag ttgaagatcg gctacgtcaa tacccagcgt
      601 ctttttcgtg acgcaccggc agccgtgaga gccgcgaaga agatcgagca ggagttctcc
      661 aagcgggacc aggacctgca acgtctggcc aagcaggttc agggtttgca ggagactctc
      721 gaaaaaggcg gattgacgat ggctgaatcg gagcgccgtg ccaaggagaa ggatctcggt
      781 gagttgtcga gggagtttca gcgcaagcag cgggagttcc gcgaagacct gaatctgcgc
      841 cagaacgaag aaaatgcagc gatcatcgaa aaggccaaca aggcgatcaa gcagcttgcc
      901 gaaaacgaga agttcgacct gatcgttcag gatgtcgtct gggtcagtcc gaagctcgac
      961 attaccgaca aggtcatcaa agccctgtcc gaaccccagg ccgcgaagta agcgcggccg
     1021 tctgccagat tgctggggtg gatgtgtcac gaggtctgcg gctaggcgat atcgtcgccc
     1081 gtctcggtgg ggtgctgcac ggtgacgaaa cggtgttcgt ctcgcaggtc ggttcgctgc
     1141 tgtccgcggg ggcagggcag atcgcgtttc tcgccaatcc gaaataccgc gaccaactgc
     1201 atcgcacaca ggcctccgcg gtgatcgtgc cgccgccttt cgcgacggaa acgtcactac
     1261 cgcgcatcgt tgaccggaat ccctacgcct attacgcgcg cgtcgtgggc ttgctgaatc
     1321 cgcctgcgca aggtcggccc gggattcatc cgggggcggt ggttcgctcc acagtaccgt
     1381 catcggcgag tgtcggcgaa aacgttgttg tcggcgaagg cgtgtgtctt ggcgagaacg
     1441 tgatcctgca ccctggctgc gtgattggcg acggtgtgac gatcggnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnggca tcctctcgtt caagacgctg ggtgccaagc
     1621 ccgacagttc ctccctcttc tatttcgtgg gaattgacag tgcgcgtttc aagaagaccg
     1681 taaccgcggg tgaccaactg cacctgcacg tcagcatcct gcgccagatg cgcaacatct
     1741 ggaaattcaa ggccgaggca cgcgtcgacg gcgagatcgt cgccgaagcc gagctgatgt
     1801 gcgccaagag ttcgatcgtc aaggcgccgg ggtgatggcg gcaatgattc atcaaacggc
     1861 aatcgtccac gcgggtgcgc aacttggcga aaacgttgcc gttggtcctt attcgatcat
     1921 cggcgagcac gtggagattg gcgacaacac gatcgtcggc ccgcatgtgg tgatctccgg
     1981 gcggacacgc atcggctgtg aaaaccgcat cttccagttc tgctccctgg gcgaagtgcc
     2041 gcaggacaag aagtatgctg gcgagccgac ttgccttgag attggcgatc gcaacacgat
     2101 ccgggagttc tgctcgttca acctgggtac ggcgctcgat gccggcgtta ctcgtgttgg
     2161 cgacgacaac tggatcatgg cctacgtgca tatcgcgcat gattgccagg tgggtaaccg
     2221 taccgtgttt gccaacaatg cgcagttggc cggccacgtg catatcgatg actgggccat
     2281 cctcggcggc tacaccggcg tccaccagtt ctgccgggtc ggcgcgcaca ccatgactgc
     2341 cgtcggcacg gtggtcctgc aggatattcc acccttcgtg atggctgccg gcaataccgc
     2401 cagcccattc ggaatcaatg ccgaagggct caagcgccgg ggtttttcgg cggaagcgct
     2461 gctggccctg aagcgtgcct accggacgct gtacaagtct gggctgatgc tcgacgaggc
     2521 gcgcgccaaa ctggcgcagg aagtggttgc gcatcccgaa atacagcccc tgatcgattt
     2581 cctcgcggtg tcgaaacgcg gcatcattcg atgaccgcag cggggttccg gatcgccatg
     2641 gtggctggtg aggcatcggg cgatctgctc gccagtcagt tgatccaggc gctccgcgca
     2701 aggttgccga acgccgtttt ttatggcgtg ggcggtccga agatgctggg catgggcttc
     2761 gaggcctggc atccactgga gaagctggcg gttcgcggct atgtcgaggt gctccgacac
     2821 tatcgtgaaa ttgccgccat tcgcagggat ctcaaacgcc gctttctggc cgatccgccc
     2881 gatgtcttca tcggtgtgga tgcgccggat ttcaatctgg cgctcgaaaa
//
LOCUS       DAOSIT010000282         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29624_length_2917_cov_7.466457, whole genome shotgun sequence.
ACCESSION   DAOSIT010000282 DAOSIT010000000
VERSION     DAOSIT010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29624_length_2917_cov_7.466457"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLL14_07485"
     CDS             complement(<1..769)
                     /locus_tag="PLL14_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG03808.1"
                     /translation="MDWIYTLSGFVVGAIVGLTGVGGGSLMTPLLVLLFGIHPATAVG
                     TDLLYAAITKAGGTVVHARKEHVDWRITRLLAGGSIPAALLTIWALSLLPKQSATVSH
                     IISFSLGIALLLTAAAILFRSKLQQQALAHADDAAHTQLRAPITVAVGALLGVLVTIS
                     SVGAGALGVAVLFYLYPRLPAIRIIGSDLAHAVPLTLIAGLGHWLIGSVDWSLLGSLL
                     LGSLPGIWLGSHASAKVPERVLRPILAGMLLLIGSKLI"
     gene            873..1820
                     /gene="cysB"
                     /locus_tag="PLL14_07490"
     CDS             873..1820
                     /gene="cysB"
                     /locus_tag="PLL14_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HOG03809.1"
                     /translation="MKLQQFRYLVEVARRGLNVSEAAEALYTSQPGISKQIRLLEEEL
                     GVTVFERGGKRLTSITEPGRAVLEIAERILRDAENIRRVGEEYAGGDSGSLVIATTHT
                     QARYALPTVVKNFVDRHPRVRLSLHQGHPAQIAELTLKGEADIAIATEALDQYPQLLM
                     LPCYQWVHCVIAPDGHPILGEKPVSLTALSRWPLITYDSAFAGRSRINKAFELAQLTP
                     NVVLTAIDADVIKTYVGLGLGLGIIARMAFDPVRDTGLQAMAAEHLFGSNTTRIGLRR
                     GTHIRRYEYDFIELFASHLTKKAVDMAMAGARPADEYQL"
     gene            1979..2629
                     /locus_tag="PLL14_07495"
     CDS             1979..2629
                     /locus_tag="PLL14_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03810.1"
                     /translation="MRNAALCDLRGLKYLVDGPCPPEARTTRPPGQQRCDEAGQAGGT
                     TVQAQPMDSIEALSLPPPRRDLAWVGRIERWLLPGGLVIGGGVLAALFLRILRGRRAR
                     PDVEEASPRVGWTVVQLLVAASLAVPLAYKVAGLAFQRVFASFDNHDTAAPWLLAAPV
                     GLLVFVLMAGMAFALLALVLGYLFKGCTRQQGRVTTRQARGQPPKSGVHDDAQDQP"
BASE COUNT          527 a          928 c          971 g          491 t
ORIGIN      
        1 caatcagctt gctgccgatc agtagcagca tgccggccag gatcgggcgc agaacgcgtt
       61 ccggcacctt cgctgaggcg tggctgccga gccagatgcc gggcagcgat ccgagcagca
      121 ggctgccgag cagcgaccag tcgacgctgc cgatcagcca gtgccctagg ccggcgatca
      181 gcgtcagcgg tacggcgtgc gccagatcgc tgccgatgat acgaatcgcc ggtagccggg
      241 ggtaaaggta gaacaatacg gcgacgccga gcgcgccggc tccgaccgac gaaatggtta
      301 ccagcacgcc gagcagcgcg ccgacggcaa ccgtgatcgg cgcccgcaac tgcgtgtgtg
      361 cggcgtcgtc ggcgtgcgcc agggcctgct gctgcagctt gctgcgaaag aggatcgcgg
      421 cggcggtgag cagcagggcg ataccgagtg aaaaggagat aatatgcgac accgtagcac
      481 tctgcttcgg cagcagggac aatgcccaga tcgtcagcag cgcggccgga atgctgccac
      541 cggccagcag acgggtgatg cgccaatcga cgtgctcctt gcgagcgtgc accaccgtgc
      601 cgccggcctt ggtgatggct gcatacagca ggtcggtgcc gaccgccgtt gccgggtgga
      661 tgccgaacag gagcaccagc aacggcgtca tcagcgagcc gccgccgacg ccggtgagcc
      721 cgacgatggc gccgacgacg aatcccgaca aggtgtatat ccagtccatg aatcgaccac
      781 cggaaaaagt tggcgcatgg tagcagcgca gcgtttgatc gaaaaagaat attcagttag
      841 attgttataa ccaatttaat ggtgcgatag ccatgaaact ccagcagttc cgctatctcg
      901 tcgaagtggc gcgccgtggt ctcaatgtct ccgaagccgc ggaggcgctt tatacctcgc
      961 agccggggat ctccaagcag atcaggctgc tcgaagagga actcggggtg accgtcttcg
     1021 agcgtggagg caagcgcctg acgtcgatta ccgagcccgg cagggcggtg ctcgaaattg
     1081 ccgaacgcat tctgcgcgat gccgagaaca tccggcgcgt cggcgaagag tacgctggcg
     1141 gcgactccgg cagcctggtc atcgccacca cccatacgca ggcgcgctat gcgttgccga
     1201 cagtggtcaa gaactttgtc gaccggcatc cccgggttcg cctgtcgctg caccaggggc
     1261 atcccgcaca gatcgccgaa ttgacgttga aaggcgaggc cgacatcgcc atcgccaccg
     1321 aagcgctcga ccagtatcca caactgctga tgttgccgtg ttaccagtgg gtgcactgtg
     1381 tgatcgctcc cgacgggcac ccgattctcg gcgaaaagcc ggtgtcgctg accgccctgt
     1441 cgcgctggcc actgatcacc tacgactcgg cctttgccgg tcgctcacgc atcaacaagg
     1501 cttttgaact ggcgcaactg accccgaatg tggtgctcac ggcgatcgac gccgacgtga
     1561 tcaagaccta tgtcgggctg gggctggggc tggggatcat tgcccgcatg gctttcgatc
     1621 cggtgcgcga caccggtttg caggcgatgg ccgccgagca tctgttcggt tcgaacacga
     1681 cgcgtatcgg tttgcgccgt ggcacgcaca tccgccgcta cgaatatgac ttcatcgaac
     1741 tcttcgcctc gcacctgacg aagaaggccg tcgacatggc gatggccggt gcgcgaccgg
     1801 ccgacgagta tcagctctga gtgcgccggt gtaccggatc agccggggga atggagaagc
     1861 accgaggcgc cgccagcgtg ccgctttggg tcttgccggc agtgtgtcgg tggcgttgct
     1921 ggtcactgcc gcttcggcat gccgggcaga cgtaccgacc atcgccgagg agtgccggtt
     1981 gcgcaacgcc gcgctctgcg acctgcgtgg cctgaagtac ctcgtcgacg gcccctgtcc
     2041 gccggaagcc cggaccactc gcccgccagg tcagcagcgc tgtgacgagg cgggacaggc
     2101 gggggggacg acagtgcaag cacagcctat ggactcgatc gaggcgctat cgctaccgcc
     2161 acccagacgc gacctggcct gggtagggcg aatcgagcgc tggctgttgc cgggggggtt
     2221 ggtcatcggc ggcggcgtac tcgctgcgct gttcctgcgg atactccgcg ggcgaagggc
     2281 gcggcccgat gtcgaagagg cgagtccgcg cgtcggctgg acggtcgtgc aactcctggt
     2341 cgctgccagc ctggccgtgc cgctggccta taaggtggcc ggcctggcct ttcaacgggt
     2401 gttcgcgagc ttcgacaatc acgataccgc cgcaccctgg ctactcgccg ctccggtcgg
     2461 attgctggtg tttgttctga tggcgggcat ggcctttgcg ctgcttgcgc tcgtcctcgg
     2521 ctacctgttc aagggctgca cgcggcagca gggaagggtc acgacccggc aggcgcgtgg
     2581 tcagccgccc aagtccggtg ttcatgacga cgcgcaggac cagccgtaag gcgtctgtcg
     2641 tgtgcgctcc aggcacgcgc gggcggtggc gagtttcagc cacaccttgc cccttgcagc
     2701 gtcggcggcc tgagctacac ttcaaccatg ccgccacttc cacgaaggga tccctcatga
     2761 gcacaccgtc ctccgctcca tcggccaaac gcaagcctgc ggttgcccgg cgaaccgcaa
     2821 agaagtcaga tgcgcaggcg agcgccgaca gcgcacggtc gaccgagccg ggcatggtcg
     2881 atcagatgcg cgcgatggcc agccgtttgc tggacct
//
LOCUS       DAOSIT010000283         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29660_length_2914_cov_10.456803, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000283 DAOSIT010000000
VERSION     DAOSIT010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29660_length_2914_cov_10.456803"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLL14_07500"
     CDS             <1..185
                     /locus_tag="PLL14_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOG03811.1"
                     /translation="TARYVRFDMSRCPQSNPGSYPSCAIGEVAFRSADVPEPAMLGLL
                     GLGLVGLVGSRRRKAA"
     gene            complement(319..1830)
                     /locus_tag="PLL14_07505"
     CDS             complement(319..1830)
                     /locus_tag="PLL14_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOG03812.1"
                     /translation="MKTALRSEALYRAAELRSIESAAADQPLMQRAGLAAADLATALC
                     RVQGAPLLIFAGPGNNGGDAFVAARHLRERRFAVHVVFAGEGSRLPKDAAAAYKRFID
                     DGGSVLESIPTGQRWALIIDGLFGIGLQRPISGRYGEFVLAANALAARDRCPLLALDC
                     PSGLDADTGRLCGATIRASHTITFIAGKPGLHTADGPDHCGIISLAALDVDAERLTTA
                     SARTVGSDLFIDWLRPRSRNSHKGSNGSAGIIGGARSMVGAAFLAGRAALRLGSGRVY
                     LGLLDGLAQAIDPLQPELMLRQPDALLATTLNALACGPGMGSGEEAPALLERACALDI
                     PLLLDADALNLLAADHTLQQVVPARHARGAATLLTPHPTEAARLLGCDTVRVQADRAG
                     AALQLAARYQALVVLKGCGSIIATPAGRWFVNTSGNPGLATAGTGDVLSGFVVALLAQ
                     GWPALEAALAAVHLHGAAADECVAGGYGPIGLTAGELIDSARACFNRWVANGR"
     gene            1944..>2914
                     /locus_tag="PLL14_07510"
     CDS             1944..>2914
                     /locus_tag="PLL14_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOG03813.1"
                     /translation="MADILLLRGTPAVSSFRLQRLQQAVTIAVPGARIDAADYWHFAA
                     CRQPLGAIERRQLSALLEERQEDEREGGELFLVTPRIGTISPWSSKATDIASNCGLRA
                     ISRIERGIAYRLHVSRRQLSATEQSRVAALLHDRMLETVLGDFAQVAELFHHFAPKPL
                     CSVDLLAGGRAALVDANKRLGLALSDDEIDYLVDLFLRTQRDPTDVELMMFAQANSEH
                     CRHKIFNASWIVDGQMRDETLFGMIRETHQAHPAGTVVAYSDNSSVIEGAQVARFHPG
                     ADGCYGYREEQTHILAKVETHNHPTAISPFPGAATGSGGEIRDEGATG"
BASE COUNT          492 a         1024 c          920 g          478 t
ORIGIN      
        1 cgacggcacg ctacgttcgt tttgacatgt cgcgttgccc gcagtccaat cccggcagtt
       61 acccgtcctg cgcgattggc gaagtcgcct tccgcagtgc cgacgtcccc gagcccgcca
      121 tgctcggtct cctgggcctc ggcctcgtcg gactggtggg cagccgtcgc cgcaaagccg
      181 cgtaatttgt agtctgcggg tatacggccg gactgaagac cgggctgcag cgacatggtc
      241 gctgcagccc ggtctttgct tgtgcgacag gttcaggccg aagcggtcgc cggtgcacca
      301 gcagccacac cggcgacctc accggccatt cgccacccaa cggttgaagc aggcacgcgc
      361 gctgtcgatc agctcgccgg ccgtcagccc gatcggccca tagccaccgg cgacacactc
      421 gtcagcggct gcgccgtgca gatgtacagc ggccagcgcc gcttcgagcg ccggccagcc
      481 ttgcgccagc agggcgacga cgaagccgct gagcacgtcg cccgtgccgg ccgtggcgag
      541 acccggatta ccactggtgt taacgaacca gcgacccgct ggtgtggcga tgatactgcc
      601 gcaacccttc agcaccacca gcgcctgata ccgggcggca agctgcagcg ccgcaccggc
      661 acgatcggcc tgtacccgta ccgtatcgca gcccagcaag cgcgcggcct cggttggatg
      721 cggcgtcagc agcgtcgccg cgccgcgcgc gtggcgtgcc ggtaccacct gctgcaaggt
      781 gtgatccgcg gccagcaggt tcaatgcgtc ggcgtcgagc agcagcggaa tgtcgagcgc
      841 gcacgcgcgc tcaagcaggg ctggcgcttc ctcgccactg cccatccctg ggccgcaggc
      901 cagcgcgttc agcgtagtag ccagcaaggc gtcgggctgc cgcagcatca gttcgggctg
      961 cagcgggtcg atcgcctgcg ccagcccatc gagcagcccc aggtagacgc gaccgctgcc
     1021 gagccgcagc gctgcgcgtc cggccagaaa cgcggcgcca accatgctgc gcgcgccccc
     1081 gatgatgccg gcgctgccgt tgctgccctt atgactgttc ctgctgcgcg gacgcagcca
     1141 gtcgatgaac aggtcactgc cgactgtccg ggcactggcc gtcgtcaggc gctcggcgtc
     1201 gacgtcgagc gcggccagcg aaatgatgcc gcagtgatcg ggaccgtcgg cggtgtgcag
     1261 gcccggcttg ccggcgatga aggtgatcgt gtggctggcc cgaatcgtcg cgccacagag
     1321 tcttccggta tcggcgtcga gcccgctggg acagtcgagg gccagcagcg ggcagcgatc
     1381 gcgggcggca agcgcgttgg cggccaacac gaattcgccg taacgtccgc tgatcggccg
     1441 ttgcagacca ataccgaaga ggccgtcgat gatcagcgcc cagcgctgac ccgtggggat
     1501 gctctcgagc acgctgccgc catcgtcgat gaaacgcttg tacgcggcgg cggcgtcctt
     1561 cggcaggcga ctcccctcac cggcaaaaac gacatgcacc gcgaaacgcc gttcgcgcag
     1621 gtggcgtgcg gcgacgaagg catcgccacc gttgttgccc ggcccggcga agatcagcag
     1681 cggcgccccc tggacacgac aaagggcagt cgcgagatcg gccgccgcca gccccgcgcg
     1741 ctgcatcaac ggctgatcgg cggcggcgct ctcgatgctg cgcaactcgg cggcacggta
     1801 gagcgcttct gatcggagag ctgttttcat tgccggatcc tagcccaacg ggctcgactt
     1861 tgcatgccct ttgcgtcggc tataatcgcc cctcaccgtt gttcccccaa cgcagctcaa
     1921 cctgcccttc tccgcagccc accatggccg atatcctcct tctccgcggc actcccgccg
     1981 tttcgtcttt ccgtctgcag cgcctgcagc aggcagtgac cattgccgtt cccggagcgc
     2041 gcatcgatgc cgccgactat tggcacttcg ccgcctgccg acagccgctc ggcgcaatcg
     2101 aacgtcgcca gctttcggcg ttgctcgaag agcgccagga agacgaaagg gaaggcggcg
     2161 aactgtttct ggtcaccccg cgtatcggga cgatctcgcc ctggtcgtcg aaagccaccg
     2221 acattgccag caactgcggc ctgcgcgcca tcagccgcat cgaacgcggt attgcctacc
     2281 ggctgcacgt atccaggcgt caattatcgg cgaccgagca gagccgcgtc gcagccctgc
     2341 tccacgaccg tatgctcgaa accgttctcg gcgactttgc acaggtcgcg gaactgttcc
     2401 atcactttgc gccgaagccg ttgtgcagtg tcgacctgct ggccggcggc cgtgcggctc
     2461 tggttgacgc caacaagcgc ctggggctgg cgctgtccga cgatgaaatc gattacctgg
     2521 tcgatctctt cctgcggacg cagcgcgacc cgaccgacgt cgagctgatg atgttcgcac
     2581 aagccaactc cgaacattgc cggcacaaga tcttcaacgc gtcctggatc gtcgacggac
     2641 aaatgcgcga cgaaaccttg ttcggaatga tccgcgagac ccaccaggca cacccggcgg
     2701 gcaccgttgt cgcctattcc gataattcat cggtcatcga aggcgcccag gtcgcacgct
     2761 tccatcctgg tgccgatggc tgttacggct atcgggaaga gcagacacac attctcgcca
     2821 aagtcgagac acacaaccac ccgacggcga tttcgccttt ccccggggcg gcgaccggct
     2881 cgggcggcga gattcgcgac gagggcgcca ctgg
//
LOCUS       DAOSIT010000284         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29755_length_2906_cov_7.708874, whole genome shotgun sequence.
ACCESSION   DAOSIT010000284 DAOSIT010000000
VERSION     DAOSIT010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29755_length_2906_cov_7.708874"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLL14_07515"
     CDS             complement(<1..482)
                     /locus_tag="PLL14_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOG03814.1"
                     /translation="MADASIALQNFFVRFGQRQLAGPVLILLILAMMVLPLPPFVLDL
                     FFTFNIAISVIVMLVAMSVMKPLDFSVFPTVLLVTTLLRLSLNVASTRVVLLEGHTGP
                     GAAGQVIEAFGHFLVGGNYAVGLVVFTILVVINFVVITKGAGRVAEVAARFTLDAMPG
                     "
     gene            complement(475..1614)
                     /gene="flhB"
                     /locus_tag="PLL14_07520"
     CDS             complement(475..1614)
                     /gene="flhB"
                     /locus_tag="PLL14_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOG03815.1"
                     /translation="MAEESDLERTEAASARRLEQAREEGQVPRSREVGAFLILVVAAT
                     AFWLMGPWMVQRVSGIVRRGLVFDHQQARDPSLMLVRLADIALDALFSFAPLFAALVV
                     AALLSPFFLGSWNFSAQALQPDLSRLDPLKGLARLVSWSGLAELLKAVAKASLVGGVA
                     VWVLWSERGELLAMFAQSIQVGLSSAGHMLSFSFFAMVAVMLLIVAVDVPFQLWQYHD
                     KLKMSHQEVKQEGKELEGNPEVKGRIRQLQREAARKRMMAAVPAADVIVTNPTHYAVA
                     LAYKSGMSAPKVLAKGMGEVALKIRQVAADAGVPMIEAAPLARALHRHVDLDQEIPSA
                     LYAAVAEVLAYVYQLANWRQTGGSFPQPPQAITVPEHLLAEALNG"
     gene            complement(1785..>2906)
                     /locus_tag="PLL14_07525"
     CDS             complement(1785..>2906)
                     /locus_tag="PLL14_07525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOG03816.1"
                     /translation="ILAGKTDSETLQALDQAVSQLDLLVSDLQNSVMKTRMQPIGRLF
                     QKYPRIARDLARQLGKDVELALVGEETEVDKTMIEDLADPLIHLIRNAVDHGVESPEE
                     RRAAGKPAKSVIRLEARQEGDHIVLIIADDGHGMSPERIRAKAVEKNIISEEEANTLD
                     DRQSLNLIFLPGFSTMARASAVSGRGVGMDVVKTNIQKLNGAIEIRSEPGKGSVFIIS
                     LPLTLAILPVLLVLLGEQPFALPLSLVREILPIDHDRMQEVGGKETLVVRGEILPVIA
                     LARLLGWPQVQRPEYGVLMQAAERSFILAVDMFAGRDDAVIKALDDFRPRGVAGVTTL
                     SNGQIVLILDMKELLGDLTANVERDVRGVAPTRALEFSA"
BASE COUNT          652 a          923 c          817 g          514 t
ORIGIN      
        1 ttgcccggca tggcgtcgag ggtgaagcgt gcagccacct cggcaacccg tccggcaccc
       61 ttggtgatca ccacgaagtt gatcaccacc agaatggtga acaccaccaa gccaaccgca
      121 tagttgccgc cgaccaggaa atgaccgaag gcttcaatga cctgccctgc agcccccggc
      181 cccgtatgtc cttcgagcag gacgacccgc gtcgaggcca cgttcaacga caatcgcagc
      241 agcgtggtca ccagcagcac ggttgggaaa acggaaaagt ccagcggttt catcacgctc
      301 atggcgacca gcatgacgat caccgagatg gcgatgttga aggtgaaaaa aaggtccagc
      361 acaaaaggcg gcagcggcag gaccatcatc gccagaatca gcagaatcaa caccggacca
      421 gccagttgcc gctgtccaaa gcgcacgaag aaattctgca gtgcgatgct cgcgtcagcc
      481 attcagggcc tccgcgagaa gatgctccgg aaccgtgatg gcttgcggtg gctgcggaaa
      541 gctgccaccg gtctgccgcc agttggccaa ctgataaaca taagccagca cctcggcgac
      601 ggccgcatac agcgccgacg gtatctcctg gtcgagatcg acatgccggt gaagcgctcg
      661 cgccaaaggt gccgcctcga tcatcggaac ccccgcgtcg gcagcgacct gacggatctt
      721 gagggccact tcgcccattc ccttggccag caccttcggc gcgctcatgc cgctcttgta
      781 ggccagcgcg actgcatagt gagtcgggtt ggtgacgatc acgtccgccg ccggcacggc
      841 ggccatcatc cgtttgcgcg cggcctcgcg ctgcaactgg cggatgcgcc ccttcacctc
      901 ggggttgcct tcgagttcct tgccttcctg tttgacttcc tggtggctca tcttgagttt
      961 gtcgtgatac tgccagagct gaaaaggcac atccaccgcg acgatcagca gcatgaccgc
     1021 aaccatcgcg aaaaaactga aactgagcat gtggccggca ctcgacaggc cgacctggat
     1081 cgattgcgca aacatggcca acagttcacc gcgctcgctc cacaggaccc ataccgcaac
     1141 gccacccacc agagacgctt tggccacggc cttgagcagt tcagcgagcc cactccagga
     1201 aacgagacgg gccagaccct tgagtggatc caggcgactc aggtcaggct gcaacgcctg
     1261 cgcggagaaa ttccaactgc cgagaaagaa tggggacaag agcgctgcga ccaccaacgc
     1321 ggcgaacaag ggtgcaaagg aaaacagggc gtcaagggcg atatcggcca accgaaccag
     1381 catcaacgaa ggatctcgcg cctgttggtg gtcgaaaacc agaccgcgcc ggacaattcc
     1441 ggagacccgc tgcaccatcc agggccccat caaccagaaa gccgtggccg ccacgacaag
     1501 aatcaggaac gcaccgacct ctcgcgaacg agggacctgt ccctcctcac gtgcctgttc
     1561 cagacgtctt gccgacgccg cttcggttct ttcgaggtcg ctttcctcag ccatttcgaa
     1621 cttccatctc tgccaaaggt attataaaaa aacagaagtc aaaataaagg cgtatgggtt
     1681 gttatttaga cggattctcg gatagttgcg aaagagcaac agttgtcgca gagctacaaa
     1741 aacaaacggg gaccgcagcc cccgcaccag gtggtccttc ccggctaggc ggaaaattcc
     1801 agggcacgcg tcggagcaac gccgcgcacg tcacgttcca cgttcgcagt cagatccccc
     1861 agcagttcct tcatgtccag gatcagtacg atctggccat tcgacagcgt cgtcaccccg
     1921 gcgactccac gtggacggaa atcatcgaga gccttgatca cggcatcatc acggccggca
     1981 aacatatcga cggcaagaat gaagctccgt tcggcggcct gcatcagcac accgtattcg
     2041 ggacgctgca cctgtggcca gcccaaaaga cgcgcgagag caatcaccgg caggatctcg
     2101 ccacgcacaa ccaaggtttc cttgcccccc acttcctgca ttctgtcgtg gtcgatcggt
     2161 agaatctcgc gaaccaacga cagcggcagg gcaaagggct gctcaccaag caggaccagc
     2221 agaacgggga ggatcgccag ggtcagtggc aaggaaatga tgaataccga gcccttccca
     2281 ggctctgagc ggatctcgat ggcgccgttg agcttctgga tgttggtctt gacgacatcc
     2341 atcccgacgc cgcggccaga cacggccgag gcccgcgcca tcgtggaaaa gcccggtaga
     2401 aagatcaggt tcaggctctg acgatcatcg agtgtgttcg cctcctcttc gctgatgatg
     2461 ttcttctcga cggccttggc ccggatgcgc tcggggctca tcccgtggcc atcgtcggca
     2521 atgatcagga cgatatggtc gccctcctga cgtgcctcca ggcggatgac gctcttggcc
     2581 ggtttgccgg cagcccgacg ttcctcgggt gactcgacac catggtcgac cgcgttgcgg
     2641 atcaggtgaa tgagcggatc ggccaaatcc tcgatcattg tcttgtcgac ttcggtctct
     2701 tcgccaacca acgccagttc gacatccttg cctaattgtc gagccagatc gcgggcaatg
     2761 cgcgggtatt tctggaacaa tcgaccgatc ggctgcatcc gcgtcttcat caccgaattc
     2821 tgcagatccg aaaccagcag gtcgagctgg ctgaccgcct ggtcaagtgc ctgcagtgtt
     2881 tccgaatcgg tcttgccggc gagaat
//
LOCUS       DAOSIT010000285         2905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29768_length_2905_cov_9.204912, whole genome shotgun sequence.
ACCESSION   DAOSIT010000285 DAOSIT010000000
VERSION     DAOSIT010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29768_length_2905_cov_9.204912"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1399
                     /locus_tag="PLL14_07530"
     CDS             314..1399
                     /locus_tag="PLL14_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOG03817.1"
                     /translation="MRNTANDIPARRQAQQRADEIRVFQTELARLETEGVLALEESQR
                     QALAAHHASLLANYARAYDIDRDSRAKQLSLGMRIASFLGAMALAASVFFLFYQFWGR
                     FPEAAQVAILITSALGSLGLTVWIQGRDATGYFTRLAALVAFACFVLNVSMIGQIFNI
                     TPSDKALLPWAAMAFLLAYACDLRLLLAAGIACVIAYVAARVGTWSGVYWLHAGERPE
                     NFFVVAALLLAMPRLAVHRHYPGFEAIYRVFGLLTALIPMLILGHWGQISYLDLDPKL
                     IEGGYQVAGFVGSALTIWIGTRREWPETVNTGIIFFVLFLYTKFYDWWWEVMPKYLFF
                     LVLGLCAVLILLLLKRLRGAADRGNNA"
     gene            1396..>1676
                     /locus_tag="PLL14_07535"
     CDS             1396..>1676
                     /locus_tag="PLL14_07535"
                     /inference="COORDINATES: protein motif:HMM:NF027432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HOG03818.1"
                     /translation="MIAWNRGRTLAAGAALILLTNIVALSGVIINRTGEPESRLMLSA
                     RELSLPYRGYRQAENSGLALTLTWRVLDADEEGSDYGSSNYGVQPTWLDA"
     assembly_gap    1677..1776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1777..2025)
                     /locus_tag="PLL14_07540"
     CDS             complement(<1777..2025)
                     /locus_tag="PLL14_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOG03819.1"
                     /translation="MSTHASHPDIIKRLKRAEGHLKSIIAMLEDSRNCLEIAQQLQAV
                     ESAVGNAKKTLVHDHIDHCLEQAVRNGSRDADDAIREFK"
     gene            complement(2039..2476)
                     /locus_tag="PLL14_07545"
     CDS             complement(2039..2476)
                     /locus_tag="PLL14_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HOG03820.1"
                     /translation="MRIGELAQRSDCDVETVRFYEREGLLDLPAREANGYRLYSEAHL
                     VQLKFVRHCRSLGMGLPDVRILRGFQANPDLACEEINQLIDRQIERIHQQLDSLQLLE
                     RQLHKLRDTCHANRKAGQCGIMRNLARAAEGGDCSCHPPAATN"
     gene            2559..>2905
                     /locus_tag="PLL14_07550"
     CDS             2559..>2905
                     /locus_tag="PLL14_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOG03821.1"
                     /translation="MNPHNQRKRVFHLHVQEKEACCASGHEHRQQPSPASRAADDHHH
                     GHPAHAHADACCSHGEVTSKRTPARHDPPPAGTRQVRYRIDHMDCPTEEGLIRNRLET
                     MPGIVRLDFRLLDR"
BASE COUNT          466 a          938 c          868 g          533 t
ORIGIN      
        1 aggggtggca tcggcgaaga ggggtttcat ggcgactccc ggaagtagcg gtgtccgata
       61 aggggacgct tgcccgggca tctcgcgccc cgccctgcga cgccgtcaag ccccctggca
      121 tgcagcagta taaggtgccg tcagcgtcgc ggaaagaggg cgactgatcc tgccggatct
      181 tgccggtcct cccgtatcga ccacggccaa cgccgcccgc ggccggccgg caaggtcgag
      241 ccgaatccgg cgcgctcccg tgcgggtgaa acgtggccga atcggcgcaa accgggcata
      301 atcgccggcg accatgcgca acaccgccaa cgatattccg gcgcgccgcc aggcgcagca
      361 acgtgccgac gagatccgcg tcttccagac cgaactggcg cgcctggaaa cggaaggtgt
      421 gctcgccctc gaagaatcgc agcggcaggc gcttgccgcc caccacgcgt cgttgctggc
      481 gaactacgcg cgcgcctacg acatcgatcg cgacagccgc gccaaacagc tctcgctcgg
      541 catgcgcatc gcctcgtttc ttggcgccat ggcgctggcg gcgagcgtat tcttcctctt
      601 ctaccagttc tgggggcgtt ttccggaagc ggcgcaggtg gccatcctga tcaccagcgc
      661 gctcggcagc ctcggcctga cggtctggat acagggtcgc gacgccaccg ggtacttcac
      721 cagactggcc gcgctggtgg cctttgcctg cttcgtgctc aacgtctcga tgatcggcca
      781 gatcttcaac atcacgccgt cggacaaggc gctgttgccc tgggcggcga tggccttcct
      841 gctcgcctac gcttgcgatc tgcggctcct gctggcggcc ggcattgcct gcgtcatcgc
      901 ctacgttgcg gcgcgcgtcg ggacctggag cggtgtctac tggctgcacg ccggcgaacg
      961 gccggagaac ttcttcgtcg tcgcggccct gctgctcgcc atgccgcgac ttgcggtgca
     1021 tcgccactac cccggtttcg aggccatcta ccgagtcttc ggtctgctga ccgcgctgat
     1081 cccgatgctg atcctcggcc actggggcca gatcagttac ctcgacctcg atccgaagct
     1141 gatcgagggc ggctatcagg tcgccggctt tgtcggcagc gcgctcacca tctggatcgg
     1201 cacgcgccgc gaatggcccg aaacggtcaa caccggcatc atctttttcg tcctcttcct
     1261 gtacaccaag ttctacgact ggtggtggga ggtgatgccc aagtatctgt tcttcctggt
     1321 actgggcctc tgcgcggtgc tgattctgct gctcctcaag cgcttgcgcg gcgctgccga
     1381 tcgaggcaac aacgcatgat cgcctggaac cgcggacgca ccctggcagc cggggcggca
     1441 ctgatcctgc tcaccaacat cgtcgccctc tccggtgtca tcatcaaccg gacaggcgaa
     1501 cccgagagcc gcctcatgct cagcgcgcgc gaactgtccc tgccctaccg cggctaccgc
     1561 caggcggaaa acagcggcct ggcgctgacg ctgacctggc gcgtgctcga tgcggacgaa
     1621 gaaggttccg actacggttc ctcgaactat ggcgtgcagc cgacctggct ggatgcnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttg aactcgcgga tggcgtcgtc
     1801 cgcgtcccgg gaaccgttgc gcaccgcctg ttcgaggcaa tgatcgatat ggtcgtgcac
     1861 cagtgttttc ttggcgttgc ccaccgcgct ctcgacggcc tgcagttgct gggcgatctc
     1921 caggcaattg cggctgtcct cgagcatggc gatgatgctc ttgaggtggc cttcggcgcg
     1981 tttcaggcgc ttgatgatgt ccgggtggga agcgtgcgta ctcatgcctt gctcctgctc
     2041 agttcgtcgc cgcgggcgga tggcacgaac aatcgccgcc ctcggccgcc cgtgccagat
     2101 tgcgcatgat gccacactga ccggcttttc gattggcgtg gcaagtgtca cgcagtttgt
     2161 gcaattgccg ttcgagcagc tgaagtgaat ccagttgctg gtggatgcgc tcgatctggc
     2221 ggtcgatcaa ctggttgatt tcttcacagg ccagatccgg gttggcctgg aagccgcgca
     2281 ggatgcgcac gtccggcagc cccatgccga gcgatcgaca gtgacgaacg aacttgagct
     2341 gtaccaggtg tgcttcgctg tagaggcggt agccgttggc ctcgcgcgcg ggcaggtcga
     2401 gcagtccttc acgctcgtag aagcgcaccg tttccacgtc gcagtcgctg cgttgtgcca
     2461 gttcgccgat cctcattgct ccctgctcca gaaagttctt gaccctgtac caactaaaga
     2521 ctgttcaatg atggcaccgt tcccggagat atgccaccat gaatccgcac aaccagcgca
     2581 aacgcgtgtt tcatctgcac gtacaggaga aggaagcgtg ctgcgcgtca gggcatgaac
     2641 atcggcagca gccgtctcca gcaagccgcg ccgcagatga tcaccatcac ggccaccccg
     2701 cgcatgcgca tgccgatgcc tgctgctcgc acggcgaggt gaccagcaag cgcactcctg
     2761 cccggcacga cccaccaccc gcgggcacca gacaggtgcg ctaccgtatc gaccacatgg
     2821 actgccccac cgaggaaggg ctgatccgca atcgcctgga gacgatgccg gggatcgtcc
     2881 ggctcgactt cagactgctc gatcg
//
LOCUS       DAOSIT010000286         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29833_length_2900_cov_12.368366, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000286 DAOSIT010000000
VERSION     DAOSIT010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29833_length_2900_cov_12.368366"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLL14_07555"
     CDS             complement(<1..707)
                     /locus_tag="PLL14_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03822.1"
                     /translation="MILRLFPKRPDHPLADGQELKRILAELHVARALNAVDEVAGWFE
                     SLKHAEDFRLDHLFDVLRQLDDAAQPHLRRLARDYLHSPHLSTIERQRLWTRSCTYLS
                     EVATVYSLCIERARLDPKSRGSVAFKASLPLLNTRLQAACCARLKWLAYRYEPPGEEL
                     WRCLGETYLAAEAAGHAQKSLQLYPAQLGLSSVAQQYLHALVFFTSSVDGLLPVQVEM
                     ADRLIAHFNLHRQQSVDR"
     gene            complement(896..2368)
                     /gene="trpE"
                     /locus_tag="PLL14_07560"
     CDS             complement(896..2368)
                     /gene="trpE"
                     /locus_tag="PLL14_07560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOG03823.1"
                     /translation="MTEAYFNRLAAEGYNRIPVTLETFADLDTPLSIYLKLANAPYSY
                     LLESVQGGERFGRYSIIGLASPTRIVVNAHQVLVLNGNRIAEREDDTNPLDFIGKYMK
                     RFRAAPTTGLPRFCGGLVGCFGYDTVRYIETRLTRSQKTDDLGIPDIVLLLSEEIAVV
                     DNLSGKLTLVVYAEPGVPGAYHKAHARLRELLASLREPVRIPPETPRSSQPAASMFGE
                     AQFRQAVVKAKHYITEGDLMQVVLSQRMSKPLAASPMALYRSLRSLNPSPYMFYFDFE
                     DFHVVGASPEILVRLEGDTVTVRPIAGTRRRGVSFEEDQALATELLADEKERAEHVQL
                     LDLGRNDAGRVARVGTIRLTENMIVERYSHVMHIVSNVEAKLRPKLSALDVLKATFPA
                     GTVSGAPKVRAMEIIDELEPVKRGIYAGAVGYLGFNGDMDLAIAIRTAIVKDGQLHVQ
                     AGAGIVADSDPASEWQETQNKAQAILRAAELAEHGLDTRC"
     gene            complement(2508..>2900)
                     /locus_tag="PLL14_07565"
     CDS             complement(2508..>2900)
                     /locus_tag="PLL14_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG03824.1"
                     /translation="FPGVLEGLQAFKALDLPLGVITNKAEAFTLPLLQRTGLFPFFDV
                     IVSGDVLPRAKPDPMPLLWASGRLGVSPVEVLMIGDSVHDFHAGRAAGCHVFLVPYGY
                     NEGRDIRELACDGIVSTLSEAARRVVHA"
BASE COUNT          552 a          868 c          949 g          531 t
ORIGIN      
        1 cggtcgacgg actgctgccg gtgcaggttg aaatgcgcga tcaagcgatc ggccatttca
       61 acctgcaccg gcagcagtcc gtcgaccgat gacgtaaaga acaccagcgc gtgcaggtat
      121 tgctgcgcaa cactgctgag ccccaactgg gcggggtaaa gctgcagcga tttctgcgcg
      181 tgccctgccg cttcggccgc gagataggtt tctccgagac aacgccagag ttcttcgccc
      241 gggggctcgt agcgatacgc cagccacttc aggcgcgcgc agcatgctgc ctgcaagcgg
      301 gtgttgagca ggggcagcga cgccttgaaa gcaacgctgc cccgactttt gggatccaga
      361 cgggcgcgct cgatgcagag ggagtagacc gtggccactt cgctgagata agtgcaactt
      421 cgcgtccaca gccgttggcg ttcgattgtc gacaggtgcg gcgagtgcag atagtctcgc
      481 gccagacgac gcaggtgcgg ctgcgccgca tcgtcgagtt gcctgagcac gtcgaacaag
      541 tggtccagtc ggaaatcttc ggcatgcttc agcgactcga accagcccgc cacttcgtca
      601 accgcgttca gtgcccgggc gacatgcagt tcggcgagaa tcctcttcaa ctcctggccg
      661 tcggccagag gatgatcagg acgcttcgga aacagtctga ggatcattga aggtcagcct
      721 tgcgacgaat tgcatgcttg cgccaggaaa agacggtcaa ttgtaatcca ggcacgcaac
      781 ggctgtagat caaatgacac gcgtaccctg ggtaaacgta gcctctgcac gggctcggtt
      841 cagcaggcgg cggcacgctc aacccgggcg gcagactgcg gcaaacccgg ttgctctagc
      901 agcgcgtgtc gaggccgtgt tcggcaagct cggctgcccg cagtatggcc tgcgccttgt
      961 tctgggtttc ctgccattcg gaagccgggt cggagtcggc gacgatgccg gcgccggcct
     1021 gcacgtgcaa ctggccgtcc ttgacgatgg ccgtgcggat cgcgatggcc agatccatgt
     1081 cgccgttgaa accgagatag ccgacggcac ctgcgtagat tcctcgcttg acgggttcga
     1141 gctcatcgat gatctccatg gccctgacct tcggagcccc ggaaaccgtc cccgccggaa
     1201 acgtggcctt cagcacgtcg agcgcactga gctttggtct tagcttggct tcgacgttcg
     1261 agacgatatg catcacgtgt gagtagcgct cgacgatcat gttttcggtg agtctgatcg
     1321 tgccgacgcg cgcgacgcgg ccggcgtcgt tgcggccgag atcgagcagc tgcacatgct
     1381 ctgcacgttc cttctcgtcg gccagcaact cggtggcaag tgcctgatcc tcctcgaacg
     1441 aaacgccgcg ccggcgtgtg ccggcgatcg ggcggacggt gaccgtatcg ccttccaggc
     1501 ggacgagaat ctccggtgag gcgccgacca cgtggaagtc ctcgaagtcg aaatagaaca
     1561 tgtagggcga aggattgagc gagcgcagcg agcggtacag ggccatcgga ctggcggcaa
     1621 gcggtttgct catgcgctgg gacagcacga cctgcatcag gtcgccctcg gtgatgtaat
     1681 gtttcgcctt caccaccgct tgccgaaact gcgcttcgcc aaacatcgac gccgccggtt
     1741 gtgaagatcg cggcgtttcg ggcggaatgc ggacgggctc gcgcaggctg gcgagcagtt
     1801 ccctgagccg ggcgtgtgcc ttgtggtatg cgccgggaac gccaggttcg gcatagacca
     1861 ccagtgtcag cttgcccgaa aggttgtcga cgaccgcgat ttcttcggaa agcagcagca
     1921 cgatgtcggg aattcccagg tcgtcggttt tctgggagcg cgtcaggcgg gtttcgatgt
     1981 aacggacggt gtcgtagccg aagcacccga ccaggccgcc gcagaagcgc ggcagcccgg
     2041 ttgtcggagc ggcacgaaag cgcttcatgt acttgccgat gaagtcgagt gggttggtgt
     2101 cgtcttcacg ctcggcgatg cggttgccgt tcagtaccag tacctgatgc gcattgacga
     2161 caatgcgcgt cggactggcc aggccgatga tcgagtagcg gccaaagcgc tcgcccccct
     2221 gcaccgattc gaggagatag ctgtaaggcg cgttggccag tttcagatag atcgacagcg
     2281 gcgtatcgag gtcggcaaag gtttcgagcg tgaccgggat gcggttgtag ccttcggctg
     2341 ccaggcgatt gaaataggct tcagtcatcg gaattccaca aggatgtgct acaggatgcg
     2401 gcgcgcgaac cgatgcgcat cggccacatg ggctgggcgg cccccacagt caggccgcgc
     2461 gtgttacggc caggcccggg cggctgtcgg acgccggtat ggggcgctca tgcgtgtacc
     2521 acgcgtcgcg cggcttcgga aagggtcgaa actataccat cacaagccag ttcgcgaatg
     2581 tcgcgtcctt cgttgtagcc gtaaggcacc agaaacacat gacaacctgc ggcccgtccg
     2641 gcatgaaagt catgcaccga atcgccgatc atcagaacct cgaccggcga cacgccgagc
     2701 cgaccgctgg cccagagcag gggcatcggg tcgggctttg cccgcggcag cacgtcgccg
     2761 ctgacgatca cgtcaaaaaa ggggaacagc ccggtgcgtt gcaagagggg cagggtaaat
     2821 gcttcggcct tgttggtgat caccccgagc ggcaggtcca gcgccttgaa tgcctgcagc
     2881 ccctccagca cgccggggaa
//
LOCUS       DAOSIT010000287         2890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_29976_length_2890_cov_9.378483, whole genome shotgun sequence.
ACCESSION   DAOSIT010000287 DAOSIT010000000
VERSION     DAOSIT010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29976_length_2890_cov_9.378483"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="PLL14_07570"
     CDS             <1..>1082
                     /locus_tag="PLL14_07570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG03825.1"
                     /translation="NPATQATPVIFLTALGDPGDEEHGLSLGAVDYIVKPIQPAVVLA
                     RVRNQLDLKRARDILSDHNAWLEAEVSRRVAENRRLEDASREAQARLSHQLELILSTA
                     GEGIYGTDADGVVNFVNPAATEMLGFSRDELIGFSAHATFHHSRVDGSPYPQHDCPLV
                     NSLAAGIALRSQEETFWRKDGTQLPIEYSSVPIIEAERRLGAVVSLRDISERKRYLAQ
                     LERQSNFDDLTGLPNRNLLNDRLAHAIDQPRHSPEAVLAVLAIEPDRFQTVNETLGRA
                     TGDRVITELATRLAAHTQPADTLARLEGDEFVLVIEVARSELASSLAQSVVGAMARPM
                     LLAGRELTLGVSIGIALFPKDGSNGD"
     assembly_gap    1083..1182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1183..1944
                     /locus_tag="PLL14_07575"
     CDS             <1183..1944
                     /locus_tag="PLL14_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03826.1"
                     /translation="GTGFSSLSYLRRFAIDRLKIDQSFVHDINRDPENAAIALSVIAL
                     AHNLRMAVIAEGVETEAQLGFLRAHGCDEMQGYLFSRPLPASDFAQLLGGGRHVSLTA
                     DPTAPSRTLLLVDDETSILSSLKRLFRREGYAVLTAGNGAEALDLLATHPVKVIISDA
                     RMSGMDGSELLGKVRALYPDTVRLMLSGYTDLQAVTRAVNSGELYQFITKPWDDARLV
                     EAVRAAFLHYEARHKARHGLASPVDAAPAREESPP"
     gene            1941..>2890
                     /locus_tag="PLL14_07580"
     CDS             1941..>2890
                     /locus_tag="PLL14_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03827.1"
                     /translation="MNSQAPAGLTSARPVRAEMLRVVVAYAVFATLWILFSDTLIGSL
                     SDNPGTLVMLGTLKGLAFVALTSLLLYALLRRVGGTDEATATPVGNRRLVAWVVGLAL
                     LILLAGAAAMRQVAQRHEDLAAAQLHATARLKVGQIEAWLNERRKDIEIVRHLPALTR
                     ALRQWQRDGDAASTAFAREQLVEVQRIGGHRSMAVLDATGTARFTAGDDYHLPEDRLD
                     TAIKRALSGDTSQFADLFVDRAGGTELSFAFVAPLPATAGTPALLILLLANAGDFLLP
                     LLQVLPVPSASAETLLIRRDSDSVLLLDAPRDRDGSTRPRR"
BASE COUNT          451 a         1007 c          883 g          449 t
ORIGIN      
        1 aaccccgcaa cgcaggccac cccggtgatc ttcctgaccg cactcggcga tccgggagac
       61 gaggagcacg gactgtcgct cggcgccgtc gattacatcg tcaaaccgat ccagccggct
      121 gtggtcctgg cgcgcgtccg caatcagctc gatctcaagc gtgcgcgcga catcctcagt
      181 gaccacaacg cctggctcga agccgaagtc agccgccgcg tcgccgagaa ccgccgcctc
      241 gaggacgcca gccgggaagc ccaggcacgg ctcagccacc aactggagtt gatcctcagc
      301 accgccggtg agggcatcta cggtaccgac gccgacggcg tggtcaattt tgtcaacccc
      361 gccgcgacgg agatgctcgg cttcagccgc gacgaactga tcggtttttc ggcgcacgcc
      421 accttccatc actcgcgcgt cgacggcagc ccctatccgc agcacgactg tccgctggtc
      481 aactcgctgg ccgccggcat cgccctgcgc agtcaggagg aaaccttctg gcgcaaggac
      541 ggtacgcagt tgccgatcga gtactcgagc gtgccgatca tcgaagccga gcggcgactc
      601 ggcgccgtcg tcagcctgcg cgacatcagc gagcgcaaac gctacctggc gcaactcgaa
      661 cgccagtcca acttcgacga cctgaccggt ctgcccaacc gcaacctgct caacgaccgc
      721 ctggcgcacg ccatcgacca gccccggcac tccccggagg cggtgctggc ggtgctggcg
      781 atcgagccgg accgcttcca gacggtcaac gaaacccttg gccgcgccac tggcgaccgc
      841 gtcatcaccg agctggcgac acgcctggcg gcgcacacgc agccggccga caccctcgcc
      901 cggctcgaag gcgacgaatt cgtgctggtt atcgaggtcg cccgcagcga actcgccagc
      961 agcctcgcac agtccgtggt cggcgccatg gcgcggccga tgttgctcgc cgggcgcgag
     1021 ctgacgcttg gcgtcagcat cggcattgcc cttttcccga aggacggcag caacggcgac
     1081 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcaccgg tttctcgtcc
     1201 ttgagctacc tgcggcgctt cgccatcgac cgtctgaaga tcgaccagtc ttttgtccac
     1261 gacatcaacc gcgaccccga gaacgccgcc atcgcacttt cggtgatcgc cctggcgcac
     1321 aacctgcgca tggcggtgat cgccgagggt gtcgagaccg aagcccagtt gggcttcctg
     1381 cgcgcccacg gttgcgatga aatgcagggc tacctgttca gccggccatt gccggccagc
     1441 gacttcgcgc aactgctcgg tggtggccgc cacgtcagcc tgacggccga tccgacggca
     1501 ccctcccgta ccctgctgct ggtcgacgac gagaccagca tcctctcgtc gctgaagcgc
     1561 ctgttccggc gcgaaggata tgccgtgctg actgccggca acggcgctga agcgctcgac
     1621 ctgctggcca cgcacccggt caaggtcatc atttcggatg cgcgcatgtc cggcatggac
     1681 ggcagcgaac tgctcggcaa ggtgcgcgcg ctctaccccg atacggtgcg cctgatgctc
     1741 tccggctata ccgatctgca ggcggtcacg cgtgcggtca atagcggcga gctgtaccag
     1801 ttcatcacca agccgtggga cgacgccagg ctggtcgagg cggtgcgcgc cgcgttcctc
     1861 cattacgaag cacgtcacaa ggcccggcat ggcttggcta gcccggtcga cgcggcgccc
     1921 gcccgcgagg aatcgccgcc gtgaactcgc aggcgccagc cgggctcacg tccgccaggc
     1981 cggtgcgcgc cgagatgctg cgcgtggtgg ttgcctacgc ggttttcgcc acgctctgga
     2041 tcctgttctc cgacaccctg atcggcagtc tgagcgacaa tcccggaacg ctggtcatgc
     2101 tcggcacgct caaggggctg gcctttgtcg cgttgacctc gctgctgctg tacgcgctgt
     2161 tgcggcgcgt cggcggtacc gacgaggcaa cggcaacccc ggtcggcaac cgccgactgg
     2221 tggcctgggt cgtcggactg gcgttgctga tcctcctcgc cggcgctgcg gccatgcggc
     2281 aggtggcgca gcggcacgag gacctggccg ccgcgcaact gcacgccacc gcccgcctca
     2341 aggtcggcca gatcgaggca tggctgaacg agcggcgcaa ggacatcgag atcgttcgcc
     2401 acttgccggc gcttacccgg gcgctgcgtc agtggcagcg cgatggtgac gcggccagta
     2461 ccgcgttcgc ccgcgagcag ctggtggagg tacagcgcat cggcggccac cgcagcatgg
     2521 ccgtgctcga cgccaccggc acagcgcggt ttaccgccgg cgacgactac caccttcctg
     2581 aagatcgcct cgatacggcg atcaagcgcg cgctgagcgg tgatacatcg cagttcgcgg
     2641 acctcttcgt ggatcgtgcc ggtggtaccg agctgtcgtt cgctttcgtc gccccgctgc
     2701 cggctaccgc cggcaccccg gctctgctca ttctgctgct cgccaatgca ggcgatttcc
     2761 tcttgccgct gctgcaggtt ctgccggtac ccagcgccag tgccgaaacc ctgctgatac
     2821 gccgcgacag cgattcggta ctgctgctcg acgcgccgcg cgaccgcgac ggcagcacgc
     2881 ggccacggcg
//
LOCUS       DAOSIT010000288         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30101_length_2881_cov_6.502831, whole genome shotgun sequence.
ACCESSION   DAOSIT010000288 DAOSIT010000000
VERSION     DAOSIT010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30101_length_2881_cov_6.502831"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1303
                     /locus_tag="PLL14_07585"
     CDS             182..1303
                     /locus_tag="PLL14_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03828.1"
                     /translation="MRIDSSLLAEFYSEAAAIVATEKTLHAIFYRLLTMRGIAPEVIG
                     REFGSPRPFDVTLFADHTCRQPVAVIEFKGGAYGSRNSLSDEIKADGFCEDMEKLEPF
                     AHAGVSVWFICVDHAELGTSIAPERRKTIADQCRRRGINFAYHCTEESNAIVVAGAAP
                     VMSVPVEVARTDGTRLPADALFSRNGDVMQGFAQQALSIAGSEDDYCGLLYTALRAAG
                     CGLRQISLETYFCFARRGTKAERMQDRPDLCVFDPGVRGRFNLYQGGKRRQPLDAHKL
                     YELRALAELKGGRSLATKSDAQLMRVYLADVAKLGEWRRRVTLARHELGLAIDLPTRY
                     VFVAVDIRPRCLAADACASLYASAGETGTDVVYVGRKSS"
     gene            1340..1960
                     /locus_tag="PLL14_07590"
     CDS             1340..1960
                     /locus_tag="PLL14_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03829.1"
                     /translation="MKKPNSVRSLEDLGRVRLSSSFYFRDFLYSEISNLHGIPNIPDD
                     PDLAIAAGMRLCEDLLEPLQATFGRLAIRSAYRAPAVNDFGNAHALRCANNEKNRAAH
                     IWDQRDENGCMGATACIVVPWFADRYASTGDWTPLAWWIHDHLPYDSAVFFPRLAAFN
                     LRWHEAPRRRIDSYVAPKGCLTQSGMANHAGSHADAYADFPIYRKG"
     gene            complement(2187..>2881)
                     /locus_tag="PLL14_07595"
     CDS             complement(2187..>2881)
                     /locus_tag="PLL14_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG03830.1"
                     /translation="IEGTDRPGLERLLRYCARPAFALERLREVDAEHLVYESIKPGPG
                     GSISLMLTPLELIERLAALIPPPRRHRHRYYGVLAPNAPLRAQVTALVGVPESPTTPL
                     VTATETETEPIAAQITAPGASSDSEEQAEEALLRRAARYAWALLLARIYEVFPLVCPR
                     CGGEMRIIAFITDAAAVREILSHLGEPTSPPCLMKARAPPLWEMQGANMGEDEAQAQS
                     APEYQFDQRIAW"
BASE COUNT          518 a          858 c          916 g          589 t
ORIGIN      
        1 gtcttgttga cccggattaa ctgggggggc cgggtgccga tccgtgcgac tatgcgattg
       61 gaaatatcgt atgatattcg cgatgcctga aaatcggtgc caacttcggc aattgtcgct
      121 tttccgtgcc gccgaagcgg accccgacac tgatcggacg tttgcctgag gcattcaact
      181 gatgcgtatc gactcttcct tactcgccga gttctattcc gaagcggcag ctattgtcgc
      241 caccgaaaag acgctgcacg ccattttcta ccgtctgctc acgatgcggg gtatcgcgcc
      301 ggaagtaatc ggccgtgagt ttggctcgcc acggccgttc gatgtcactc tgtttgccga
      361 tcatacgtgc cggcagccgg tcgccgtgat cgagttcaag ggcggtgcct atggatcccg
      421 aaattcgctc agtgatgaga ttaaagctga cgggttctgc gaggacatgg aaaagctcga
      481 accgtttgcg cacgccggtg tgtcagtttg gtttatttgt gttgaccatg ccgagttggg
      541 gacctcgatt gccccggaac gacggaaaac catcgccgac caatgccggc gccgcggcat
      601 caactttgct tatcattgca ccgaggagtc caatgccatc gtagtggcag gcgccgcgcc
      661 ggtgatgtct gtgcctgttg aggttgcacg gacggatggc acgcgcctgc ccgccgatgc
      721 cctgttcagc cggaatggcg acgtgatgca gggatttgca caacaggcac tctcaatcgc
      781 cggtagcgag gatgattact gtggcctgtt gtacacagca ctacgtgcgg ccggttgtgg
      841 actcaggcag atttcgctcg aaacgtactt ctgctttgcc cggcgtggca cgaaggcaga
      901 gcggatgcag gatcgccccg acttgtgcgt cttcgatccc ggggtgcgtg gtcgcttcaa
      961 cctgtaccag ggaggcaagc gacgtcagcc gctcgatgcg cacaagctct atgaactgcg
     1021 ggcactggcc gaactcaagg gcgggcggtc cttggccact aagagtgatg ctcagctgat
     1081 gagagtctac ctggccgacg tcgccaaact tggcgagtgg cgaaggcgcg taacgttggc
     1141 tcgtcacgag cttggcctgg ctatcgatct gcctacccgt tacgtattcg tggcagtcga
     1201 tatccgcccg cggtgccttg ccgccgatgc ttgtgcgagt ctgtacgcgt cagccggcga
     1261 aacaggcacc gatgtggtct acgtcggccg caagtcgtcg taataggtgt gcgggtcgat
     1321 tggtcttgct gggggtcgaa tgaagaagcc gaatagcgtc cggtcactcg aggatctggg
     1381 ccgggtacgc ctttcttcgt ccttctactt ccgcgatttc ctgtacagcg agatctcaaa
     1441 tctgcatgga attccgaata ttcccgacga tccggacttg gctatcgccg cgggtatgcg
     1501 cctttgcgaa gacctgctcg agccactgca ggccacattc ggtcgactgg cgattcggtc
     1561 ggcttaccgc gcaccggccg taaacgactt cggcaacgcc catgcgctcc gttgtgccaa
     1621 caacgaaaag aaccgggctg cgcatatctg ggaccagcgc gatgaaaacg gctgtatggg
     1681 cgcgaccgcc tgcatcgtcg taccatggtt cgcggaccgt tacgcgagta ccggagactg
     1741 gacgccgctt gcatggtgga ttcatgacca tctgccctac gacagcgccg tgtttttccc
     1801 ccgactcgct gcgttcaact tgcgttggca tgaggcgccg agacgccgca ttgatagcta
     1861 tgtcgccccc aagggctgcc tgacgcagtc cgggatggca aaccacgctg gctctcatgc
     1921 cgatgcctac gccgactttc cgatttatcg taagggctga cggaaggata ggagattcaa
     1981 cgccacttcc gcaagtgttc caggctctga taggccctga cagcagtcgt gagtcgggga
     2041 aagcgtgccg acggagcctg aaaccctcgg cagaatcgca gcacgaggat ggccatgggt
     2101 cgccgcggtg gccgacgagg ccgccgcggc gacccatggt gcaagcgttc cctgacgagc
     2161 ccgatcggat cgtcttcgtg cggcctttac caggcgatgc gctgatcgaa ttggtactcc
     2221 ggcgccgact gagcctgcgc ttcgtcctcg cccatgttgg cgccctgcat ctcccagagc
     2281 gggggcgcac gggctttcat cagacatggc ggcgaggtcg gttcgcccag atggctcagg
     2341 atctcgcgga cggcagcggc gtcggtgatg aaggcgatga ttcgcatctc gccgccgcag
     2401 cgtggacaga cgagcggaaa gacctcataa atccgcgcga gcagcagcgc ccaagcgtaa
     2461 cgggcggcgc gacgcaggag cgcttcctct gcctgctcct cgctgtcgga cgacgcgcct
     2521 ggggcggtga tttgcgccgc gattggctca gtctccgtct cagtcgctgt aacaagcggc
     2581 gtggtcggac tctctggcac gccgacaagc gcggtgacct gtgcgcgtag cggtgcgttg
     2641 ggcgccagta cgccgtagta ccgatgccga tgacggcgcg gcggcggaat cagcgccgcc
     2701 agacgctcga tcagttcgag cggtgtgagc atcaggctga tgctgccgcc gggacccggc
     2761 ttgatgctct cgtagaccaa gtgttcggcg tcgacttcac gcaagcgctc cagcgcaaag
     2821 gccgggcgag cgcagtagcg cagcagccgt tccaggcccg ggcggtcggt gccttcgatg
     2881 c
//
LOCUS       DAOSIT010000289         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30158_length_2876_cov_10.813896, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000289 DAOSIT010000000
VERSION     DAOSIT010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30158_length_2876_cov_10.813896"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..582
                     /locus_tag="PLL14_07600"
     CDS             88..582
                     /locus_tag="PLL14_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOG03831.1"
                     /translation="MATHRENRAIALSSEWLFDIVADVERYPEFLPLVREARILNRYA
                     EAYETEQSLVLGLLAHRFRTRTELKRPHAIVVTSADRSFCRFDIRWTFSERHDEHCQV
                     DFALDCTTRSLFLMPVIELLVMPMATSMVAAFEARAYALAADAGAAAGRRVSHRVEEN
                     PRDP"
     gene            699..2546
                     /locus_tag="PLL14_07605"
     CDS             699..2546
                     /locus_tag="PLL14_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG03832.1"
                     /translation="MSQEPKSNEASSSALASTNFIRNIIEADLAAARHASRRWSGRPG
                     PAAQQLAGPPDPAQIRTRFPPEPNGYLHFGHAKSICLNFGLAQDYGGRCHLRFDDTNP
                     EKEEQEYVDSIVDSVRWLGFSWTEPASAESSDGECNLYFASNYFDWMVEFAEYLIASG
                     HAYVDSHSAEEMRASRGTLTEAGTDSPFRERSVEESMDLFKRMQKGEFPDGAHVLRAR
                     IDMSAANINLRDPAIYRIRHASHHNTGDRWCVYPMYTYAHPIEDALENITHSICTLEF
                     ADQRPFYDWLLERLAEGGLLQRPLPQQIEFSRLNLTYIVLSKRKLIQLVEEKHVDGWD
                     DPRLPTLVGARRRGYPAEGFRLFAERIGASRSESLIEYTQLEDTMREVLNESAERRVA
                     VLDPLRLIIDNYPQDQREEAFAPNHPLKPELGKRAMPFGRELWIEREDFMVQPSKGYH
                     RLYPGNMARLRYGYVVRCTGYDCDASGAVMAVHCDYLPDSRSGTSGADMYKVKGNLHW
                     VAVAEAYLAEVRLYDRLFAVPSPGARRDGDAEDLERDFRDDINPNSIRVIKAWLEPAL
                     KDARAEERFQFERHGYFVADRVDSQPGAPVFNRTVTLKDSWSAKPGRQR"
     gene            complement(2569..>2876)
                     /locus_tag="PLL14_07610"
     CDS             complement(2569..>2876)
                     /locus_tag="PLL14_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOG03833.1"
                     /translation="NGIVAEVIDYLKTPPDREELRHLLRLLGMRPAELVRRGELVFKE
                     HYAARTLDDEQWLDALLAHPILIERPIIVRGDRAVLGRPPEKVLELLQGSISVGVDR"
BASE COUNT          545 a          907 c          872 g          552 t
ORIGIN      
        1 gccaagagcg agatccgccc ggccatggtc gagatgatga acaacctgac gcgtttcagt
       61 cgtccgaagg gttgagccgc aagcggcatg gcaacccatc gtgagaaccg tgccattgcg
      121 ctatcttcgg agtggctgtt cgatatcgtt gcggatgtcg aacgctatcc ggaattcctg
      181 ccgctggtcc gcgaagcgcg gatcctaaac cgttatgcgg aggcatatga aaccgaacag
      241 agcctggtac tcggtctgct ggcacatcgc ttccggacgc gcaccgaact caagcgcccg
      301 cacgccatag tggtcacatc ggcggatcgt tctttttgcc ggttcgacat tcgctggact
      361 ttctcggagc gccacgacga gcactgtcag gtcgactttg cgcttgactg cacgacgcgc
      421 tcgctctttc tcatgccggt catcgagctc ctggtcatgc ccatggcaac cagcatggtg
      481 gctgccttcg aggcgagagc gtacgcgctg gctgccgacg ccggtgctgc ggcgggacga
      541 cgggtgtcgc acagggtcga ggagaatccg agagatcctt gacgacgggc gcctgaacgc
      601 gcgtgcgctg agttcaatgg actaccgggc aggatttctc gcagtcctgc aggcatcgcc
      661 ggttaaaatg cgaggcttct cgaatcaagg actgcaccgt gagccaggag ccaaaaagca
      721 acgaggcaag ttcatctgca cttgccagta ccaacttcat ccgcaacatc atcgaagccg
      781 atctggccgc cgccagacac gcgtcgcgca gatggtcggg tcgccccggc ccggccgcac
      841 aacaactcgc gggaccgccc gatccggcgc agatccgcac gcgttttccg cctgagccca
      901 acggttacct gcatttcgga catgccaagt cgatctgcct caacttcgga ctggcccagg
      961 attacggcgg ccgctgtcat cttcgtttcg acgacaccaa tccggaaaag gaagagcagg
     1021 aatacgtcga ttcgatcgtc gattcggtgc gctggctcgg tttttcctgg accgagccag
     1081 catcggcgga gtcatcggac ggcgagtgca acctctattt cgcgtccaac tacttcgact
     1141 ggatggtcga gttcgccgaa tacctgatcg ccagtgggca tgcgtatgtc gatagtcaca
     1201 gtgccgagga aatgcgtgcc agccgcggca cgctcaccga agccggcacc gactctccgt
     1261 ttcgcgagcg cagcgtggag gagagcatgg atctcttcaa gcgtatgcaa aagggtgagt
     1321 ttccggacgg cgcgcacgtt ctgcgcgcgc gtatcgacat gtccgccgcg aacatcaatc
     1381 ttcgtgatcc ggcgatctat cgaatccgtc atgccagtca tcacaatacc ggcgacagat
     1441 ggtgcgttta cccgatgtac acctacgccc acccgatcga ggacgcgctg gagaacatca
     1501 cgcactcgat ctgtacgctc gaatttgccg accagcggcc tttctacgac tggctgctcg
     1561 agcgcctggc cgagggcggt ctgttgcagc gaccgctgcc gcagcagatc gagttctcgc
     1621 gcctgaacct gacctacatc gtgctctcga agcgcaagct gatccagctc gtcgaggaga
     1681 agcacgtcga cggttgggat gacccgcgat tgccgacact cgttggcgct cgccgacgcg
     1741 gctaccctgc cgagggtttt cgcctttttg ccgagcgcat cggtgcttcc cgttcagaat
     1801 ccctgatcga gtacacgcaa ctcgaagaca ccatgcgcga ggtgctcaat gaatcggccg
     1861 agcgccgtgt tgccgtgctc gacccactcc ggctgatcat cgacaactac ccgcaggacc
     1921 agcgcgaaga ggccttcgct ccgaaccatc cgctgaaacc cgaactgggc aagcgggcga
     1981 tgccttttgg tcgggaactg tggatcgagc gcgaggactt catggtgcag cccagcaagg
     2041 gctaccaccg tctctatccc ggcaacatgg cccgcctgcg ctatggctac gtcgtcagat
     2101 gcacgggtta cgactgcgat gccagcggcg ctgtcatggc cgtgcactgc gactacctgc
     2161 cggactccag gagtggcacc agtggcgccg acatgtacaa ggtgaagggc aatctgcact
     2221 gggttgcggt ggcggaggcc tatctggccg aggttcgtct ctacgaccga ctgttcgccg
     2281 ttccttcacc cggtgcgcgg cgtgatggcg atgccgagga tctcgaacgc gatttccgcg
     2341 acgacatcaa cccgaacagc attcgggtca tcaaggcctg gctcgagccc gcactgaagg
     2401 atgccagggc cgaggagcgt tttcagttcg aacggcacgg ttacttcgtt gccgaccgcg
     2461 tcgattcgca gccaggtgcg ccggtgttca accggacggt gacgctcaag gattcgtggt
     2521 cggccaagcc cggccgacag cgctgagtct actgacctac ggctgtcgct accggtcgac
     2581 tccgacggag atgcttccct gcagcaactc cagtaccttc tccggcggcc gtccgagaac
     2641 ggcccggtcg ccgcgcacga tgatcgggcg ctcgatcagg atcggatgcg caagcagtgc
     2701 atccagccat tgctcgtcgt ccagggtacg cgccgcgtag tgttccttga ataccagctc
     2761 gccgcggcgc acgagttcgg ctggtcgcat gccgagcagg cgcagcaagt ggcgcagttc
     2821 ttcccggtcc ggaggcgtct tcaggtagtc gatgacctcg gccacgatgc cgttgt
//
LOCUS       DAOSIT010000290         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30171_length_2875_cov_10.575532, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000290 DAOSIT010000000
VERSION     DAOSIT010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30171_length_2875_cov_10.575532"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLL14_07615"
     CDS             complement(<1..480)
                     /locus_tag="PLL14_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG03834.1"
                     /translation="MPWLSISIQTDCLHAEALADALLDAGALAVSIEDADAGTSAEQP
                     QFGEPGSISRPGWEHSRVVALLAPDTDAGLLLSACAAQAGLDELPAFAQETVAEQDWV
                     RLTQSQFEPIRVSERLWIVPSWHQAPDPEAIVLVLDPGMAFGTGSHPTTRLCLEWLER
                     "
     gene            complement(496..1860)
                     /gene="accC"
                     /locus_tag="PLL14_07620"
     CDS             complement(496..1860)
                     /gene="accC"
                     /locus_tag="PLL14_07620"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOG03835.1"
                     /translation="MFGKVLIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVRL
                     ADESVCIGPAPSAQSYLNVPAIISAAEVTDAQAIHPGYGFLSENADFAERVESSGFVF
                     IGPRPETIRLMGDKVSAKEAMRITGVPCVPGSEGALPEDPKEIVQIAKAVGYPVIIKA
                     AGGGGGRGMRVVHTEAALINAVNMTRSEAQSFFGNPNVYMEKYLENPRHVEIQVLADS
                     FRNAVYLGERDCSMQRRHQKIIEEAPAPGIATRHIVRIGERCAEACRRINYRGAGTFE
                     FLYENNEFYFIEMNTRIQVEHPVTELISGVDLVAEQIRIAAGEKLRFRQRDLSFRGHA
                     IECRINAEDPYTFVPSPGKISFYHPPGGPGIRVDSHIYQGYTIPSHYDSMVAKVIAYG
                     DSRDQAIRRMRIALSEMSIEGIKTNIALHQELMHDARFMEGGTSIHYLEHRLAAKDEV
                     KKGG"
     gene            complement(1876..2328)
                     /gene="accB"
                     /locus_tag="PLL14_07625"
     CDS             complement(1876..2328)
                     /gene="accB"
                     /locus_tag="PLL14_07625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOG03836.1"
                     /translation="MDLRKLKKLIDLVEESSITELEINDAEERVRIVKYGGALAQQAY
                     ALPPQAPLHAAPAGAAPPANAAEEPPLPEGDAVKAPMVGTFYRASSPGAEPFVEVGSV
                     VKVGDTLCIIEAMKLLNEIEAERAGTVKAILIENGQPVEYGEPLFIVG"
     gene            complement(2413..2847)
                     /gene="aroQ"
                     /locus_tag="PLL14_07630"
     CDS             complement(2413..2847)
                     /gene="aroQ"
                     /locus_tag="PLL14_07630"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOG03837.1"
                     /translation="MLVLHGPNLNLLGTREPKHYGSSTLAEINLALAHRAAAAGVHLE
                     DFQSNHEGALIERVHAASTQGVRYLIINPAAYTHTSIALRDAIAASAIPFVEVHLSNV
                     HARESFRHHSCFSDLAIGVITGLGSEGYLLALEYLLRRISVE"
BASE COUNT          562 a          853 c          888 g          572 t
ORIGIN      
        1 ccgctcaagc cactccagac acaggcgagt cgtcggatgc gaacccgtgc cgaaagccat
       61 gcccggatcg agtaccagga caatcgcctc gggatcgggg gcttgatgcc atgagggaac
      121 gatccacagg cgttccgata cgcgaatcgg ctcgaactgc gactgggtca atcgcaccca
      181 atcctgctcg gcgacggttt cctgcgcaaa cgccggcaac tcgtcgaggc cggcctgcgc
      241 tgcgcaggcg gacagcaaca gcccggcatc ggtatccggc gccagcagcg cgaccacgcg
      301 cgaatgctcc caaccgggcc tgctgatcga gccgggttca ccgaactgtg gctgctcggc
      361 ggaggtcccg gcatcggcat cctcgatact caccgccaag gctccggcgt cgagcagcgc
      421 gtcggcgagc gcctcggcgt gcaggcagtc ggtctggatg ctgatagaga gccagggcat
      481 cgatttcggt ggtcgtcagc cgcccttttt gacctcgtcc ttggctgcga gccgatgttc
      541 gaggtagtgg atactcgtac caccctccat gaaacgggca tcgtgcatga gttcctgatg
      601 caaagcgata ttcgtcttga tgccttcgat gctcatttcc gagagtgcaa tccgcatgcg
      661 gcggatggcc tgatcgcggc tgtcaccgta ggcaatcacc ttggccacca tcgagtcata
      721 atgcgacgga atcgtgtagc cctgataaat atgcgagtca acgcggatgc caggcccgcc
      781 cggcggatga tagaagctga tcttgccagg ggaaggcacg aaggtgtagg gatcttccgc
      841 attgatacgg cattcgatgg cgtggcctcg gaaactgagg tcacgctgcc ggaagcgaag
      901 cttttcacct gcggcaatcc ggatctgctc ggctacgaga tcaacgccgg agatcaattc
      961 cgtgaccggg tgctcgacct ggatgcgcgt gttcatctcg atgaaataga actcgttatt
     1021 ctcgtagagg aactcgaagg taccggcacc gcgatagttg atgcgccggc acgcttcggc
     1081 acaacgctcg ccgatacgca cgatgtgccg tgtagcgata ccgggggctg gtgcctcctc
     1141 gatgattttc tgatggcggc gctgcataga gcagtcgcgt tcgccgaggt acacggcatt
     1201 cctgaaactg tcggcaagca cctgaatctc gacatgccgg ggattttcca ggtacttctc
     1261 catataaacg ttggggttgc cgaaaaaact ctgggcctcg gagcgggtca tgttcacggc
     1321 attgatcagt gccgcttcgg tatgcacgac gcgcatgccg cgtccgccgc cacctccagc
     1381 cgccttgatg atcaccggat agccgacggc cttggcaatc tgcacgattt ccttcgggtc
     1441 ctcgggaagt gcgccctcgg aacccggaac acagggcacg ccggtaatcc gcatggcctc
     1501 cttggcagaa accttgtcac ccatcaggcg gatggtttct gggcgtgggc cgatgaaaac
     1561 gaagcctgat gactccaccc gttccgcaaa gtcggcgttc tcggagagaa aaccgtaacc
     1621 cgggtggatg gcctgcgcgt ccgtgacttc ggccgcagaa atgatcgccg gtacgttgag
     1681 atagctctgg gcggatggcg ccggaccgat acagaccgac tcgtccgcca atcgaacgta
     1741 cttggcctcg cggtccgcct cggaatgcac gaccacggtc ttgataccca gttcgcggca
     1801 ggcccgctgg atgcggagtg cgatttctcc gcgattggcg atgaggactt tcccgaacat
     1861 ggtggacagt tctccttagc cgacgatgaa caagggttca ccgtattcga ccggctggcc
     1921 gttctcgatg agaatggcct tgaccgtgcc tgcgcgctcg gcttcgatct cgttcaacaa
     1981 cttcatggct tcgatgatgc acagcgtgtc tccaaccttg acgacactgc caacctcgac
     2041 aaacggttct gctcccggac tcgaggcgcg gtagaaggtg ccgaccatcg gggccttgac
     2101 ggcatcgcct tccgggagcg gcggctcctc tgcggcgttg gcgggcggtg cagcccccgc
     2161 tggcgcggcg tgcagcggag cctgtggagg cagggcgtag gcttgttgcg cgagagcccc
     2221 accgtatttg acgatacgga ccctttcttc ggcgtcgttg atctcgagct cggtaatgct
     2281 cgactcttcg acgaggtcaa tcagtttctt cagcttacgc agatccatgg acggtctccg
     2341 gagggttgtt cttgcttgcg ttcaaacctg gaaaaaggca gcagacacaa gccgtagatg
     2401 aaatcgaagg cctcactcga cagagattcg gcgcaacagg tattcgagcg caagcaggta
     2461 accctcgctg ccgaggccgg taatgactcc aatcgccaag tcggaaaaac acgagtgatg
     2521 gcgaaacgat tcgcgtgcgt gtacgttgga gagatggact tcgacaaacg gaatggcgct
     2581 cgcagcaatg gcatcacgca gcgcgatgct agtgtgtgtg taggccgctg ggttgatgat
     2641 caggtagcgc accccttggg tactcgcggc gtgcacccgc tcgatcagcg ctccctcgtg
     2701 attgctctgg aagtcctcca ggtgcacgcc ggctgctgca gctctgtggg caagcgccag
     2761 attgatctcg gcaagcgtgc tcgaaccgta atgtttgggt tcacgagtac caaggaggtt
     2821 gagattgggt ccgtgcagga ccagcacggc agccgtgtcc ggcaacttcc cggat
//
LOCUS       DAOSIT010000291         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30242_length_2870_cov_9.771581, whole genome shotgun sequence.
ACCESSION   DAOSIT010000291 DAOSIT010000000
VERSION     DAOSIT010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30242_length_2870_cov_9.771581"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /gene="hpnE"
                     /locus_tag="PLL14_07635"
     CDS             complement(<1..973)
                     /gene="hpnE"
                     /locus_tag="PLL14_07635"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOG03838.1"
                     /translation="MKAAIVGGGWAGLSTAVELAKAGVGVTVFEAAKQLGGRARSVDL
                     HGHRLDNGQHLLVGAYCETLRLMREVGADPERLLKRLPLELRFPASQPRPFRMRLPRL
                     PAPWHLAAGLLSARGSSLTEKISAVRFMRCLQDADYRLGDDCTVSALLDGHGQRGSLR
                     QRLWEPLCLAALNTAPARASAQIFVNTLRDSLGGGRAATDLLLPAADLDRLFPEAAAH
                     FIRARGGEIRLSTRVAAIDDGFTICGEPFDYAVVAVAPQHAPVLLAGHAQTRGIAQLL
                     AGYAFEPIATLYCGYPPQVCLPFPMLGLDDRPAGRIGQWVFDRGALCG"
     gene            complement(970..1803)
                     /gene="hpnD"
                     /locus_tag="PLL14_07640"
     CDS             complement(970..1803)
                     /gene="hpnD"
                     /locus_tag="PLL14_07640"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HOG03839.1"
                     /translation="MTPDEYCQQKAARSGSSFYYSFLFLPPQQRRAITALYAFCREVD
                     DVVDECQDPQVAATKLAWWRQELARLPAGKPQHPVTQALQAVSTEFHLPEEQLQEIID
                     GMEMDLQQSRYLDFKALSLYCYRVASVVGLLAAEIFGYQDRKTQKYAHDLGVAFQLTN
                     IIRDVGEDARRGRIYLPIDELQRFQVTAADILNARHSDNFRRLMDFQIERAESYYAQA
                     IGALPAIDRRAQRPGLVMAAIYRTLLDEIKRDGCQVLSQRTSLTPIRKLWIAWRTWVR
                     G"
     gene            complement(1847..2656)
                     /gene="hpnC"
                     /locus_tag="PLL14_07645"
     CDS             complement(1847..2656)
                     /gene="hpnC"
                     /locus_tag="PLL14_07645"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOG03840.1"
                     /translation="MPVDHYENFPVASLLLPRRLRRPIEAIYRFARAADDIADEGDAT
                     DAQRLQGLAEFAGALDRIERGEHPAQADFAELAAVIHAWRLPVALFRDLLDAFAQDVV
                     TRRYADYPQLLDYCRRSANPVGRLLLHLVGRADAENLRRSDCICSALQLVNFWQDIAI
                     DWQKERVYLPQADLERFGIGEAQIAAGCWSAEWAALLAFETGRTRRLMNEGAPLVHQL
                     PGRMAWEIRLTVQGGLRILERIERVRGDVFAQRPVLGVRDWLRIGGRALRM"
     gene            2784..2868
                     /locus_tag="PLL14_07650"
     tRNA            2784..2868
                     /locus_tag="PLL14_07650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2818..2820,aa:Leu,seq:tag)
BASE COUNT          493 a          955 c          976 g          446 t
ORIGIN      
        1 taccgcagag tgcgccacgg tcgaacaccc attgcccgat ccgaccggca gggcggtcgt
       61 cgagcccgag catcgggaag ggcaggcaca cttgcggcgg atagccgcag tagagcgtgg
      121 cgatcggctc gaaggcgtag ccggcgagca gctgcgcgat gccccgcgtc tgcgcgtgcc
      181 cggcgagcaa taccggcgca tgctgagggg cgacggcgac gaccgcgtag tcaaagggct
      241 cgccacagat cgtgaacccg tcgtcaatcg cggcgacacg ggtcgacagg cgtatttccc
      301 cgccacgcgc gcggatgaag tgcgccgcgg cttccggaaa gagtcggtcg aggtccgctg
      361 ccggcagcag caggtcggtc gcggcgcggc caccgccaag gctgtcgcgc agcgtgttta
      421 cgaagatctg cgccgaagcc cgggccggcg cggtattcag cgcggccagg cacagcggct
      481 cccagaggcg ctgacgcagg ctgccgcgct ggccatggcc atcgagcaag gccgacaccg
      541 tgcaatcgtc gccgaggcgg tagtccgcgt cctgcaggca acgcatgaaa cgaacggcgc
      601 tgattttctc ggtcagactg ctgccgcgcg cgctcagcaa acccgccgcc agatgccacg
      661 gtgccggcag ccgcggcagc cgcatccgga agggacgcgg ctggctggcc gggaagcgca
      721 gttcgagtgg caggcgcttc aggagccgct cgggatcggc gccgacttcg cgcatcaggc
      781 gcagggtctc gcaataagcg ccgaccagga ggtgctgccc gttgtcgagc cggtggccgt
      841 gcaggtccac gctgcgcgct cgcccaccga gctgcttcgc ggcctcgaaa acggtgaccc
      901 cgaccccggc ctttgccagt tcgaccgccg tggacagccc cgcccagccg ccgccgacga
      961 tcgcggcctt catccccgca cccaggtccg ccaggcgatc cacagcttgc ggatcggcgt
     1021 cagcgaagtg cgctggctga ggacctgaca gccatcccgc ttgatctcgt cgagcagcgt
     1081 gcgatagatc gcggccatca ccagcccggg gcgctgcgct cggcggtcga tcgccggcag
     1141 tgcgccgatc gcctgcgcgt agtagctctc ggcgcgctcg atctggaagt ccatcaggcg
     1201 ccggaagttg tccgaatggc gggcgttgag gatgtcggcg gcggtgacct gaaaacgctg
     1261 caactcgtcg atcggcagat agatgcgacc cctgcgcgcg tcttcgccga cgtcgcggat
     1321 gatgttggtg agctggaacg cgacgcccag atcgtgcgcg tatttctggg tcttgcggtc
     1381 ctggtagccg aagatctccg cggcgagcag accgacgacg ctggccacgc gataacaata
     1441 gagcgagagc gccttgaaat cgaggtagcg cgattgctgc agatccatct ccatgccgtc
     1501 gatgatctcc tgcaactgct cctccggcag atggaactcg gtgctgaccg cctgcagcgc
     1561 ctgcgtcacc ggatgctgcg gcttgcccgc cggcagccgc gccagctcct gccgccacca
     1621 ggccagcttg gtggcggcga cctgcgggtc ctggcactcg tcgacgacat cgtcaacctc
     1681 gcggcaaaag gcgtacagtg ccgtgatcgc gcgccgttgc tgtggcggca ggaagagaaa
     1741 gctgtagtag aagctcgagc cactgcgggc cgccttctgt tggcagtatt cgtcgggggt
     1801 cattcgcaat ctcgggattt ccacagggtc gccattctcc cacgacttac atgcgcaggg
     1861 cgcgtccacc gatgcgcaac cagtcgcgca cgcccagcac cggccgctgc gcgaacacat
     1921 cgccgcgaac ccgctcgatc cgctccagaa tgcgcaggcc gccctgcacc gtcagccgga
     1981 tttcccaggc catgcggccg ggcagttgat ggaccagcgg ggcaccttca ttcatcaacc
     2041 ggcgggtccg cccggtttcg aaagcgagta gcgctgccca ctcggcgctc cagcagccgg
     2101 ccgcgatctg cgcttcgccg atgccgaagc gttcgagatc ggcctgcgga agatagaccc
     2161 gttccttctg ccagtcgatg gcgatgtcct gccagaaatt caccagttgc agcgcgctgc
     2221 agatgcaatc cgagcggcgc agattctcgg cgtcggcgcg gcccaccaga tgcagcagca
     2281 ggcggccgac cggattggcc gagcgacgac aataatccag aagctgcggg tagtccgcgt
     2341 agcgcctggt gacgacatcc tgcgcgaagg cgtcgagcag gtcacggaac aacgccaccg
     2401 gcaggcgcca cgcgtggatg accgccgcca gctccgcgaa gtccgcctgg gccggatgct
     2461 cgccacgttc gatgcggtcg agcgccccgg cgaattcagc caggccctgc aggcgctgcg
     2521 catcggtggc atcaccctcg tcggcaatgt catcggcagc gcgcgcgaaa cgatagatcg
     2581 cctcgatcgg tcggcgcagg cgtctcggca gcaggagcga ggcaacagga aagttttcgt
     2641 agtggtcgac aggcatcgcg gcgaagtctg ctgcgtctgg agcagcccag gtgctttgag
     2701 ggctgacagt atagggtcgg ggcggttaga atactgctcc gtcgcgcgat tcgcggcggc
     2761 agcaggtctc ggtgatggac cgtgcccagg tggcgaaatt ggtagacgca gcaggtttag
     2821 gtcctgccgc cgcaaggtgt gggggttcga ttcccttcct gggcaccaaa
//
LOCUS       DAOSIT010000292         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30269_length_2868_cov_7.984714, whole genome shotgun sequence.
ACCESSION   DAOSIT010000292 DAOSIT010000000
VERSION     DAOSIT010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30269_length_2868_cov_7.984714"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLL14_07655"
     CDS             complement(<1..596)
                     /locus_tag="PLL14_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG03841.1"
                     /translation="MPQVINTNVSSLNSQRSLNTSQASLTTSLQRLSSGLRINSAKDD
                     AAGLAISARMTSQINGLNQASRNANDGISLAQTAEGGLQSITESLQRMRELSVQASNA
                     TNTATDRAALQQEVDTLVQQINTVAGQTAFNGVKLLDGTFNSQSFQVGANTGESITIN
                     SIASAKANSLGVGTTSTYTTSESATVTNGAISTGGITV"
     gene            1129..1872
                     /gene="surE"
                     /locus_tag="PLL14_07660"
     CDS             1129..1872
                     /gene="surE"
                     /locus_tag="PLL14_07660"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOG03842.1"
                     /translation="MRILLCNDDGYFAPGIEHLARALSDMAEITVVAPERDRSGASNS
                     LTLDRPLSLKRAANGFYYVNGTPTDCVHLAVTGMLEGLPDMVVSGINIGANMGDDTIY
                     SGTVAAATEGFLLGVPSVAVSLCSKSGENFTTAARVARGLVEVLQRQALCEPVLLNVN
                     VPDVTYEQLRGTVVTRLGKRHKAEPVLRAVSPRNETLFWVGAAGAAQDAGEGTDFYAV
                     LNHQVSVTPLQIDLTHNGQLSMVREWLAR"
     gene            1869..2531
                     /locus_tag="PLL14_07665"
     CDS             1869..2531
                     /locus_tag="PLL14_07665"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOG03843.1"
                     /translation="MMPGRPHTGFGMTSQRTRARMIERLREQGIVDEEVLAAIAAVPR
                     HVFVEEALASRAYEDTALPLGFSQTISQPYVVARMIEALRAGRDLGKTLEIGAGCGYQ
                     AAVLAQLTDEVYAVERIAPLLAKAKLNLRAIKQLKVRLKHADGQFGLPEAAPFDTIIV
                     AAAAAHVPRALLQQLAPNGRMILPLGTATQQLVLIERRAEAYVETRLDAVRFVPLLSG
                     VE"
     gene            2528..>2868
                     /locus_tag="PLL14_07670"
     CDS             2528..>2868
                     /locus_tag="PLL14_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOG03844.1"
                     /translation="MKDQGILFRYGLALACTAVLVLDGCASKSPAPVVERGGQVGAQQ
                     TPGAAPLASKDVYLVKRGDTLYSIALDHGLDYKDLVAWNAIENPNRILVGQPLRVRAP
                     GAAAAAEGTVV"
BASE COUNT          476 a          826 c          949 g          617 t
ORIGIN      
        1 ttgaccgtga tgccgccggt cgagatggcg ccgttggtga ccgttgccga ttcactcgtg
       61 gtgtaagtcg aggtcgtccc gacgccgagc gaattggcct tggcgctggc aatcgaattg
      121 atcgtgatcg attcaccggt gtttgcgccc acctggaatg actgcgagtt gaacgtaccg
      181 tcgagcagtt tcacgccgtt gaaagcggtt tggccggcaa cggtattgat ctgctgcacc
      241 agggtgtcga cttcctgctg caatgccgcg cggtcggtgg cggtgttggt cgcgttcgag
      301 gcctgcaccg aaagttcgcg catccgttgc agcgattcgg tgatgctctg caggccgcct
      361 tcagcggtct gcgccaggga gatgccgtcg ttggcgttgc gtgaggcctg gttgagaccg
      421 ttgatttgcg aggtcatccg ggcggagatg gccaatccgg cggcgtcgtc cttggcgctg
      481 ttgatgcgca ggccggagga caggcgctgc agggatgtcg tcagcgaagc ttgcgacgtg
      541 ttgagactac gttgcgagtt gagcgacgat acgttggtgt tgataacttg gggcattttc
      601 cttctcctat cactgtcgaa ttgactattg gttgccgggg ttctgccttg cgaggcgttt
      661 cctggaccat cctgtgtggc gatcgtttcg cttacatctt ttcttacgac ccaccgccgg
      721 aaaactttag tgattttctt ctggcctgcc gtatgactgc tttatcggcg gtccgctggc
      781 gaagttgagc gtgctccgca ggatgccgcg tgcgtcggta cggctggtgt gggcaatgct
      841 catcgtcagg tcgtctgtgg cagcgtgtga ccgcttcggc tgcaagtcgg tgttcagtga
      901 tcgtcgcagg cggttataat ggcttccggt ttcggcagta gtgcggcctg cgccccaggc
      961 cacctttcag tacgcctgcg ttcgacgaac gccgggtgac aacagtagag ggttgcggcc
     1021 gacaggctgg cgatagggta ggcggtagcc tcgcggttgg tcagcaggcc ggcagatcgc
     1081 cggttttttt gtccctttgc ccgtgtaagt cgatgttcgt ggagtgctat gcggatcctg
     1141 ctctgtaacg atgacggtta tttcgcaccg gggatcgaac atctggcgcg cgcgctgtcc
     1201 gatatggcgg aaatcacggt ggtagctccc gagcgcgacc gcagcggcgc cagcaattct
     1261 ttgacgctcg atcgtccgct gtcgctcaaa cgtgctgcca atggcttcta ctatgtcaac
     1321 ggcaccccca ccgattgtgt ccacctggcc gtcaccggca tgcttgaagg gctaccggac
     1381 atggtggtgt cggggatcaa catcggcgcc aacatgggcg acgacacgat ttattcgggg
     1441 accgtcgcgg cggctaccga aggcttcctg ctcggcgttc cgtcggtggc cgtttccctg
     1501 tgcagcaaga gcggcgaaaa cttcacgact gcggcacggg ttgctcgcgg actggtcgaa
     1561 gtattgcaga gacaggcact atgcgaaccg gtactgctca acgtcaatgt tcccgacgtg
     1621 acttacgagc aactgcgtgg caccgtggtc actcgcctgg gcaagcggca caaggctgaa
     1681 ccagtgctgc gtgcggtatc gccgcgcaat gaaaccttgt tctgggtggg tgcggctggt
     1741 gccgcgcagg atgccggtga gggcacggat ttctacgccg tgctcaacca tcaggtttcg
     1801 gtgactccac tacagatcga cctgacgcac aacgggcaat tatcgatggt gcgcgagtgg
     1861 ctggcgcgat gatgcccggg cgcccgcata ccggtttcgg catgacctcg caacgcacgc
     1921 gcgcgcgcat gatcgaacgt ctgcgcgaac aggggatcgt ggacgaagag gtgctggcag
     1981 cgatcgccgc cgtgcctcgg cacgttttcg tcgaggaggc gctggcctcg cgcgcctacg
     2041 aggatacggc cttgccgctg ggtttctcgc agaccatctc gcagccctat gtcgtcgcgc
     2101 ggatgatcga agcgctgcgt gccgggcgtg atctcggcaa gactctggaa atcggggccg
     2161 gttgcggtta tcaggctgcg gtgctcgcgc aactgaccga cgaggtctat gcggtcgaaa
     2221 ggatcgcgcc cttgctggcc aaggccaagc tcaacctgcg cgcgatcaag cagctcaagg
     2281 tccgcctgaa gcacgccgac ggtcagttcg gactgccgga agcggcgccg tttgacacga
     2341 tcatcgtcgc ggcggcggcg gcgcacgttc cgcgcgcact cctgcagcaa ctcgcaccca
     2401 atggccggat gattctgcca ttgggtacgg cgacacaaca gcttgtgctg atcgagcgac
     2461 gcgccgaggc ttacgtcgaa acccgcctgg atgctgttcg cttcgttccg ttattatcgg
     2521 gagtcgaatg aaggatcaag gtattctctt tcgctacggc ctggctctgg cctgcacggc
     2581 ggtgctcgtt ctcgacggct gcgccagcaa atcgccggca cctgtcgtcg aacgtggagg
     2641 acaggtgggt gcgcaacaaa ctcctggtgc cgccccgctg gccagcaagg acgtgtatct
     2701 cgtcaagagg ggcgatacgc tgtactcgat cgcgctcgat catgggctcg actacaagga
     2761 cctggtggcg tggaacgcca tcgagaatcc gaaccgcatc ctggtcgggc agccgctgcg
     2821 ggtacgcgcg ccgggtgctg cagccgctgc cgaagggacc gtcgtcaa
//
LOCUS       DAOSIT010000293         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30293_length_2867_cov_3.266003, whole genome shotgun sequence.
ACCESSION   DAOSIT010000293 DAOSIT010000000
VERSION     DAOSIT010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30293_length_2867_cov_3.266003"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1579
                     /locus_tag="PLL14_07675"
     CDS             <1..1579
                     /locus_tag="PLL14_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG03845.1"
                     /translation="EITGYSAEECVGRTPKLLSSGRHDAAFYQAMHESLARTGTWQGE
                     IWNRRKNDEVFPEWLTISAVRDSEGCVTHYVSTLMDITLRKAAQEEIRHLAFYDPLTG
                     LPNRRLLHDRLRVAMAASARRDRQGALLFIDLDNFKTLNDTLGHAMGDLLLQQVAKRL
                     SGCVRDRDTVARLGGDEFVVMLEDLGTQSQEAAKQSRIVGEKILGVLNRPYDLAGSEY
                     HNTPSVGITLFGGQQEDIEELMKRADLAMYEAKATGRNTFRFFDPQMQAVVTARVALE
                     RDLREALQKQEFFLCYQPQVDSAGRIVGAEALLRWQHRQRGFVSPAEFIPLAEETGLI
                     LPLGLWVLETASAQAAVWAAEAGREDFTVSVNVSARQLLQPNFVEQVLGVLGNTGTNP
                     HNLKLELTESMLLDKNQEIIAKMTALKERGVGFSLDDFGTGYSSLSYLKRLPLDQLKI
                     DQSFVRDLLTDPNDEAIARTIVALASSLGLSVIAEGVESVEQRDVLAAQGCHTYQGYL
                     FSRPLPLADFNLFLQRH"
     gene            complement(1650..1820)
                     /locus_tag="PLL14_07680"
     CDS             complement(1650..1820)
                     /locus_tag="PLL14_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03846.1"
                     /translation="MDAGSLANLDWGAVALVAIIGGGLVWGLFFFLRQNREDLDSLQE
                     TLASDRDDEEPR"
     gene            complement(1979..>2867)
                     /locus_tag="PLL14_07685"
     CDS             complement(1979..>2867)
                     /locus_tag="PLL14_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOG03847.1"
                     /translation="AELAALTATPAARIQPPPAITPTPLADCVVWFSESHRFASTSGI
                     GRLAADINAGRGAEACDWLAGTTDLSVGWLPDGSADLEAATRQAIRDGYQPYREALMH
                     PHADPATCRRQVFAAFDRFRVLCAQRETARGVHAINALVSRHLQQSVGDTHATVTGSP
                     WYLGRPVMVVRNDYVLKLFNGDIGLCLPDDAGELMVWFPGQDAGFRPVAPIRLPEHDT
                     AFAMTVHKAQGSEFESVLLLLPEQTNRVVTRELLYTGVTRASKRVCLVAGQEVLVNAC
                     ANRSRRHSGLIARLGETGP"
BASE COUNT          518 a          915 c          933 g          501 t
ORIGIN      
        1 cgagatcacc ggctacagcg cagaggaatg cgtcggacgc acgccgaagc tgctcagctc
       61 cggtcgccac gacgctgctt tctatcaggc gatgcatgag agtcttgcgc gcacagggac
      121 ctggcaaggc gagatctgga accggcgcaa gaacgacgaa gtcttcccgg agtggctgac
      181 gatctccgcc gtcagggaca gcgagggctg tgtcacccac tacgtatcga cgctgatgga
      241 catcaccctg cgcaaggcgg ctcaggaaga gatccggcat ctggcctttt acgaccccct
      301 gaccgggctg ccgaatcgcc gcctgctgca tgaccgcctg cgggtggcca tggccgccag
      361 cgcccgtcgt gaccggcagg gtgcactgct gttcatcgac ctggacaatt tcaagacgct
      421 caacgatacg ctcggtcatg ccatgggtga tctgctcctg caacaggtcg ccaagcgctt
      481 gtcgggctgc gttcgggacc gggataccgt cgcccgtctc ggcggtgacg aattcgtggt
      541 catgcttgag gatctcggca cgcaatcgca ggaggccgcc aagcagagcc ggatcgtcgg
      601 tgagaagatt ctcggcgttc tcaatcgccc ctatgatctg gccgggtcgg agtaccacaa
      661 tacgccgagc gtcggcatca ccctgtttgg tggccagcag gaggatatcg aggaactgat
      721 gaagcgggcc gatctggcca tgtacgaggc caaggcgacc ggtcgcaata ccttccgttt
      781 cttcgatccg cagatgcagg ccgtggtcac cgcgagggtt gctctcgagc gggatctgcg
      841 cgaggcgctg caaaagcagg agttcttcct gtgctaccaa ccgcaggtcg acagcgctgg
      901 ccgcatcgtc ggtgccgagg cgctgctccg ctggcaacat cggcagcgtg gcttcgtctc
      961 gcccgccgaa ttcattcctc tcgccgagga aaccgggctc attctgccgc tgggcctgtg
     1021 ggtgctggaa accgccagtg cgcaggcggc cgtctgggcg gccgaggccg gccgagagga
     1081 cttcacggtt tccgtcaatg tcagtgcccg ccagctcctg cagcccaact tcgtcgaaca
     1141 ggtgctgggg gtccttggca acaccggtac caacccgcac aacctcaagc ttgaactgac
     1201 cgagagcatg ttgctcgaca aaaaccagga gatcatcgcc aagatgaccg ctctgaaaga
     1261 gcggggcgtc ggcttctcgc tcgacgattt tggcaccggc tactcgtcgc tgtcgtatct
     1321 taagcggctc ccgctcgatc agttgaagat cgaccagtcg ttcgtcaggg acctgctgac
     1381 ggaccccaat gacgaggcaa ttgcacgcac gatcgtcgcc ctggccagca gcctcggcct
     1441 gtcggtcatc gccgagggcg tggaaagtgt cgaacagcgc gacgttctgg cggcacaggg
     1501 ctgccacacc tatcagggct acctcttcag ccggccgcta cctttggctg acttcaacct
     1561 gtttctccag cggcattgac cacggctttc ccgcgtaacg aggcaatcgc ctcgcgccgc
     1621 gggtctgccg ccgactcctg gtcacgagtt catcggggtt cctcgtcgtc acgatcagac
     1681 gccagcgtct cctgcaggct gtcgaggtcc tcgcggtttt gccgcagaaa gaaaaaaagg
     1741 ccccacacca agccgccacc gatgatggcg accagtgcga cagcgcccca gtccaggttg
     1801 gccagcgatc cagcgtccat cggtctttcc tcagtatgcc cgggcccatt atgccacccg
     1861 cagcgccgac caatgcattt caaccttgac tgacggaaca gcctgtggcg ttgccggaat
     1921 cgggaacccg ggcagagtga tggcgcagtg aatggacagc acgcttgcgc gggtccgatt
     1981 acggtccggt ctcacccagc cgggcgatca ggccggaatg gcggcgggat cggttggcgc
     2041 aggcattgac cagaacctcc tggccagcga ccaggcagac tcgctttgat gcccgcgtga
     2101 cgccggtgta gagcagctcg cgcgtcacca cccggttggt ctgttcggga agcagcaaga
     2161 gtaccgactc aaattcggag ccctgcgcct tgtgtacggt catggcaaag gcggtgtcgt
     2221 gttccggtag acggatcggc gcgaccggcc ggaaaccggc gtcctgcccg gggaaccaga
     2281 ccatcagctc accggcatcg tccggcaggc agaggccgat gtcgccgttg aacaacttca
     2341 ggacgtagtc attcctgacc accatcaccg gacgtccgag ataccacggc gaaccggtga
     2401 cggtggcgtg ggtgtcgccg accgattgct gcaggtgccg gctgacgagc gcgtttatgg
     2461 catggacgcc acgcgccgtc tcgcgctgcg cgcacaggac gcgaaagcga tcaaaggcgg
     2521 caaacacctg gcggcggcac gtcgccgggt cggcgtgtgg gtgcatgagg gcttctcgat
     2581 agggctgata gccgtcgcga atcgcctgcc gtgtcgcggc ttcgaggtcg gcgctgccgt
     2641 cgggcagcca gccgaccgac agatccgtgg tgccggccag ccagtcgcag gcctcggcgc
     2701 cgcggccggc attgatgtcc gcagccagac ggccgatgcc ggaggtgctg gcgaaacggt
     2761 ggctttccga gaaccagaca acgcagtcgg cgagcggggt cggcgtgatc gctggcggtg
     2821 gctggatgcg cgccgccggc gtcgcggtga gcgcggcgag ttcggca
//
LOCUS       DAOSIT010000294         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30541_length_2849_cov_10.610594, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000294 DAOSIT010000000
VERSION     DAOSIT010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30541_length_2849_cov_10.610594"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLL14_07690"
     CDS             complement(<1..1067)
                     /locus_tag="PLL14_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03848.1"
                     /translation="MHVRRLLYLSAHQLTAFKWQSGVLSEEGSFDATEAGKLGFANYL
                     AGNRKSIFSILANVSDEGFHIETIPFLRGADRTAIVRRKLGQLYFSAALTTSMSLGHQ
                     KSRRKDERIMLAALTNNEVFAPWLTALASAESPLAGVYSLPLLGPVLLRKLGITDERC
                     LLLTIQDQSIRQSYLEKGELFFSRLTPLHNSSIGGIAQTFATEARKLQQYLVSQRLIG
                     RQQPINAYLLAHANTSKALESSCVDSDNLSFTILDIEACAQQCSFKTLPADTRCEPLF
                     LQLLAADPPRTQFASDAQRHDYHLWLIRSVLQGAGAVALFGCLLWSGRQLYEAYQLNQ
                     EVEVITAETSLARRRYEDILKT"
     gene            complement(1072..2274)
                     /locus_tag="PLL14_07695"
     CDS             complement(1072..2274)
                     /locus_tag="PLL14_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG03849.1"
                     /translation="MPLYTYKAVNPDGRLVFGRIDAINLVDLELRLRRMELDLVSGEP
                     LSNRSLLGGGGVPRREIIHFCFHLQQLVRAGVPVLEGLTDLRDSLEHPRFREVVASLI
                     ESIEGGQTLSQAMDNHRRVFNRVFVSLIRAGETTGRLPEVLQSLNESLKWEDELASQT
                     KKMVAYPAFVGSIVLAATFFLMVYLVPQLKLFVKNMGQVLPMQTKILFFVSDLMVAYW
                     YLLLLLPAVAALAVRFLLHSNPLARLRFDAIKLRVPVLGNILRKIVLSRFANTFALLY
                     SSGIPILESIRTTQDVVGNLVIRQGLERVEQLIGEGQNVTAAFHSTGFFPPLVIRMLR
                     VGENTGALDDALLNVSYFYNRDVRESVQKMQQLIEPLLTVVMGGLLGWIMLSVLGPVY
                     DVISKIKA"
     gene            complement(2280..>2849)
                     /locus_tag="PLL14_07700"
     CDS             complement(2280..>2849)
                     /locus_tag="PLL14_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG03850.1"
                     /translation="RSMMRQDPDIILVGEVRDAETAEMALRAAMTGHQVYSTLHTNSA
                     IGAIPRLLDIGILPDIMAGNIIGIIAQRLVRRLCVHCKIAYQAEAHEARLLSNLVEGP
                     RPVIYRPGGCERCDFQGYRGRLAIMELLRINGDMDELIARRSTVREMRQLATRQGFAT
                     LADDGLRRVLDGSTSLEEIGRVVDLTDRM"
BASE COUNT          556 a          859 c          921 g          513 t
ORIGIN      
        1 gtcttgagga tgtcctcgta gcgccgtcgc gccaggctgg tttccgccgt gatcacttcg
       61 acttcctggt tgagttgata ggcctcgtag agctgcctgc ctgaccatag caggcagccg
      121 aacaacgcga cggcacccgc tccctgcagc accgaacgga tcagccataa atggtaatcg
      181 tggcgttgcg cgtcgctggc aaactgggtg cgcggcggat cggcagccag caactgcaga
      241 aacagcggct cgcagcgggt atccgcaggg agcgtcttga aactgcattg ttgcgcgcag
      301 gcctcgatgt cgagaatggt gaaggacagg ttgtcgctgt cgacacaact gctctccagt
      361 gccttgctgg tattcgcgtg cgccagcagg taggcgttga tcggctgctg acgaccgatg
      421 agacgctgac tgacgagata ctgctgcagt ttgcgcgcct cggtggcgaa ggtctgcgcg
      481 atgccgccaa tgctgctgtt gtgcagcggg gtgaggcggc tgaaaaacag ctcccccttt
      541 tccaggtagc tctgccggat gctctggtcc tgaatggtga gcaacaggca acgctcgtca
      601 gtgataccga gcttgcgcag caacacaggc cccagcaagg ggagcgaata gacaccggcg
      661 agaggcgact cggcactggc cagggcggtc agccagggtg caaagacctc gttgttggtc
      721 agcgcggcga gcatgatgcg ctcgtccttg cggcgtgact tctgatgtcc gagagacatc
      781 gacgtggtca gcgcggcgct gaagtagagc tgcccgagct tgcgtctgac gatcgccgtt
      841 cgatccgcgc cgcgcaggaa ggggatggtc tcgatatgga agccttcgtc ggagacattg
      901 gcgagaatcg agaaaatgct cttccggttt cctgcgaggt agttggcaaa gccaagcttc
      961 ccggcttccg tggcgtcgaa ggaaccctcc tcggacagca ctcccgattg ccacttgaag
     1021 gccgtgagct gatgcgcgct gaggtagagg aggcgacgga catgcatggc gctaggcctt
     1081 gatcttgctg atcacgtcgt acaccggtcc gagcaccgac agcatgatcc acccgagcag
     1141 gccgcccatc accacggtca gcagcggctc gatcaactgc tgcatcttct ggacggattc
     1201 cctgacatca cggttataga agtaactgac attcaggagc gcatcatcga gcgcgccggt
     1261 gttttcgccg acgcgcaaca tgcggatcac cagcggcggg aagaagccgg tactgtgaaa
     1321 cgccgccgtc acgttctgcc cctcgccgat cagttgttcc acccgctcga ggccttgccg
     1381 gatcaccaga ttgccgacga catcctgcgt cgtccggatc gattcgagaa tcggtatgcc
     1441 cgacgagtag agcagggcga aggtgttggc aaagcgcgag aggacgatct tgcgcaggat
     1501 gttcccgagc accggcaccc gcagcttgat agcgtcgaag cgcaggcgcg ccagcgggtt
     1561 gctgtgcagc aggaatctca cggccagcgc ggcgacagcc ggcagcagca gcagcaggta
     1621 ccagtaggcg accatcaggt cggagacgaa gaacaggatc ttggtctgca tcggcagcac
     1681 ctgccccatg ttcttgacga acagcttgag ttgcggcacc agataaacca tcagaaagaa
     1741 ggtggcagcg agaacgatcg agccgacgaa ggccgggtaa gcgaccattt tcttcgtctg
     1801 cgacgcgagt tcgtcctccc acttgagcga ttcgttcagg ctctgcagga cttccggcaa
     1861 gcgaccggtc gtctcgccgg cccggatcag gctgacaaag acccggttga agacgcgccg
     1921 atgattgtcc atggcctgcg acagggtctg gccaccctcg atcgactcga tcaggctcgc
     1981 caccacctcc cggaaacgcg gatgttccag actgtcgcgc aggtcggtga gtccttcgag
     2041 gaccggcacg cccgcgcgca ccagttgctg caggtgaaag cagaagtgaa tgatctcccg
     2101 acgcggcacg ccaccaccgc ccagcaggct gcggttgctg agcggttcgc cgctgaccag
     2161 gtcgagctcc atgcgccgca gtcgcagttc gaggtccacc aggttgatgg cgtcgatcct
     2221 gccgaagacc aggcgcccat cggggttgac cgccttgtag gtatagagcg gcaccggtgc
     2281 tacatccggt cggtgaggtc gacgacgcgg ccgatttcct cgagcgaggt ggagccgtcg
     2341 agaacccgtc gcagaccgtc atcggcgagg gtggcgaaac cctgacgagt cgccagctgc
     2401 cgcatttcac ggacggtact gcggcgcgcg atcagctcgt ccatgtcacc gttgatgcgc
     2461 agcagctcca tgatcgccag acgcccacga taaccctgga aatcgcaacg ctcgcagccg
     2521 ccggggcggt agatgaccgg gcgtggccct tcgaccaggt tgctcagcag acgggcttcg
     2581 tgggcctcgg cctggtacgc gatcttgcag tgcacacaca ggcgtcgcac caggcgttgc
     2641 gcaatgatgc cgatgatgtt cccggccatg atgtccggca gaatgccgat atcgagcagg
     2701 cgcggaatgg cgccgatcgc tgaattggtg tgcagggtcg aatacacctg atggccggtc
     2761 atcgccgcac gcagggccat ttcggcggtt tcggcgtcgc ggacttcgcc gacgagaatg
     2821 atgtccgggt cctggcgcat catcgagcg
//
LOCUS       DAOSIT010000295         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30569_length_2847_cov_10.379298, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000295 DAOSIT010000000
VERSION     DAOSIT010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30569_length_2847_cov_10.379298"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..421)
                     /gene="hypA"
                     /locus_tag="PLL14_07705"
     CDS             complement(80..421)
                     /gene="hypA"
                     /locus_tag="PLL14_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOG03851.1"
                     /translation="MHEMSLAMGVLQIVEDAARAQRFRRVRSVLLEIGELSMVEAEAM
                     RFCFDAVSRGTLAEGAALNVVEVAGQGLCFNCNMTVPLAALYDPCPACGGHPVQATGG
                     TEMRVKELEVE"
     gene            complement(414..557)
                     /locus_tag="PLL14_07710"
     CDS             complement(414..557)
                     /locus_tag="PLL14_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03852.1"
                     /translation="MEIKIDSKAFSMLREDSLARLAGWLDEDPLPLSLDPCAPKEVGI
                     TNA"
     gene            631..1272
                     /gene="ccmA"
                     /locus_tag="PLL14_07715"
     CDS             631..1272
                     /gene="ccmA"
                     /locus_tag="PLL14_07715"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HOG03853.1"
                     /translation="MLEASNLECVRGEQRLFASVSFRLEGGEMLSLQGRNGSGKTSLL
                     RMLCGLTPPANGEIRWRGQPIDKLGEEFRSELFYLGHHNAIKDDLTPLENVVASTTLA
                     QEALDERAALDALERVGLRGREDLACRYLSQGQKRRTALARLVHERRALWVLDEPYVA
                     LDAAAVELVAGLVGAHLQRGGLAVLTTHQAVAVAAGGVRELWLGTDDAAHNPL"
     gene            1276..1944
                     /gene="ccmB"
                     /locus_tag="PLL14_07720"
     CDS             1276..1944
                     /gene="ccmB"
                     /locus_tag="PLL14_07720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOG03854.1"
                     /translation="MLKLIRAVISRDLRLAMRRQADIVAAMFFFIIVVSLFPLGVGPE
                     PDQLRKLAPGVLWVAALLATMLSLPRLFAEDHRDGTLEQLALAPQPLGLIVIGKIVAH
                     WLVAGLPLVVLAPVLGIQFDLTGDALAVLTLSLLIGTPALSGIGAIGAALTLGVRGGG
                     VLLSLLVLPLYIPVLIFGAGAVDATVTGLGAQAHLSLLGALALGGVFFAPWPTAAALR
                     IALE"
     gene            1945..2694
                     /gene="ccmC"
                     /locus_tag="PLL14_07725"
     CDS             1945..2694
                     /gene="ccmC"
                     /locus_tag="PLL14_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HOG03855.1"
                     /translation="MSNRLINWFRYASPQTFYPLAGKLIPWFWTLAAIFGVAGLTVGF
                     LLAPIDEQQGEGYRIIFLHVPAAWMSMFIYLVMAFWAATGLAFNTRLSGMMASALAPT
                     GALFAFLSLWTGALWGKPMWGTWWVWDARLTSELILLFLYIGFIALQAAIDDPRRADK
                     AGAILALVGVVNIPIIYFSVKWWNTLHQGASVSLTRAPSMATMMLWGMLLCTLACWMY
                     SIAVALMRVRMIMLERERHTDWVHALLIDPE"
     gene            2732..>2847
                     /gene="ccmD"
                     /locus_tag="PLL14_07730"
     CDS             2732..>2847
                     /gene="ccmD"
                     /locus_tag="PLL14_07730"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HOG03856.1"
                     /translation="MGRHGLYVWGSVIVMALLMLAEPMLLRHGRKQLIARLRR"
BASE COUNT          471 a          893 c          899 g          584 t
ORIGIN      
        1 gcggacacgg ccttgccggc gacccgggtt tccccggtgc cacaaccgca ggttgtacac
       61 attgctgtct cctgtattgt tattcgactt ccagttcctt cacccgcatc tcggtaccac
      121 cggttgcctg aaccgggtgg ccaccgcagg ctggacaggg gtcgtacaga gccgcgagcg
      181 gcaccgtcat attgcaatta aagcataaac cctgccctgc cacctcgact acgttcaggg
      241 ccgcgccttc ggccagcgta ccgcggctga cggcatcgaa acagaaacgc atggcttcgg
      301 cttcgaccat cgacagttcg ccgatttcga ggagcaccga ccggacgcgc cgaaagcgct
      361 gcgcgcgcgc cgcgtcctcg acgatctgca ggacccccat ggccagcgac atttcatgca
      421 ttcgtgatcc ccacctcttt cggcgcgcaa gggtcgagcg acaaaggcag aggatcttcg
      481 tccagccagc cagcgagtcg agccagagaa tcttcacgca gcatactaaa agcctttgaa
      541 tcgattttga tttccatcaa tccggggcta aactcccgcc ttcacaataa ctcctcgccg
      601 tcctcggcca attccggact cactctcggc atgcttgaag cttcaaatct ggaatgcgta
      661 cgcggcgaac agcgcctgtt cgccagcgtc agctttcgtc tggaaggcgg tgagatgctt
      721 tccctgcagg gcaggaacgg ttccgggaaa accagccttc tgcgcatgct gtgcgggctg
      781 acgcccccgg cgaacggcga aatacgctgg cgtggccagc cgatcgacaa gcttggcgag
      841 gagtttcgca gcgaactgtt ctacctcggc caccacaacg cgatcaagga tgacttgaca
      901 ccactcgaga atgttgtcgc gtcgactaca ctggcccagg aagctctcga tgagcgtgca
      961 gcgctcgatg ccctggagcg tgtcggactc cgtggccgtg aggacctggc gtgccgctat
     1021 ctgtcgcagg ggcagaagcg ccgcaccgca ctggcgcggc tggtgcatga aaggcgtgcc
     1081 ctgtgggtgc ttgatgaacc ctatgtcgcg ctcgacgcgg cggctgtcga actggtcgcc
     1141 ggcctggtcg gtgcgcacct gcagcgcggc ggactggctg tcctgaccac ccatcaggcg
     1201 gtggcggtgg cggctggagg cgtgcgtgaa ctctggctgg gcaccgatga tgcagcgcac
     1261 aacccgctct agttgatgct aaaattgatc cgtgcggtca tcagtcgcga cctgcgactg
     1321 gcgatgagac gacaggccga catcgttgcc gcgatgttct ttttcatcat tgtcgtcagc
     1381 cttttcccac tcggcgtcgg tccggaaccg gaccagttgc gcaagctggc gcccggtgtg
     1441 ttgtgggttg ccgcgctgct ggcgacgatg ctttcgctgc cgcggctgtt tgcagaagac
     1501 caccgcgacg gcacgttgga acaactggcg ctggcaccgc aaccgcttgg cctgatcgtg
     1561 atcggcaaga tcgtagcgca ctggctggtc gccggtctgc cgctggtggt actggcaccg
     1621 gtactgggga tccagttcga cctgaccggc gatgcgctgg cagtgctgac cctgtccctg
     1681 ctgatcggca caccagccct gtcgggcatt ggcgcaatcg gcgcggcgct gaccctcggc
     1741 gtacgcggcg gtggcgtgct gctgtcgctg ctggttctgc cgctgtacat cccggtgctg
     1801 attttcggcg ccggtgcggt cgacgcgacg gtaaccggac tcggcgcgca ggcccatctg
     1861 tcgctgcttg gcgcgctggc gctgggcggc gtgttttttg cgccgtggcc cactgccgcg
     1921 gccctgagaa ttgccctgga atagatgagc aaccgcctaa tcaactggtt caggtacgcc
     1981 agcccgcaga ccttctaccc gctcgcgggc aaactgatac cctggttctg gacactggca
     2041 gcaatcttcg gcgtcgccgg gctgacggtc ggtttcctgc tggcaccgat cgacgagcag
     2101 cagggggaag gttatcggat catctttctg catgtcccgg cagcgtggat gtcgatgttc
     2161 atttacctgg tgatggcttt ctgggccgct accgggctgg ctttcaatac tcgcctgtca
     2221 gggatgatgg cctcggcgct ggcacccacc ggtgcgcttt tcgctttcct ctccttatgg
     2281 accggcgcgc tgtggggcaa accgatgtgg ggtacctggt gggtgtggga tgcgcgcctg
     2341 acatcggaat tgatcctgct cttcctctac atcggcttca tcgccctgca ggcggcgatc
     2401 gacgatcctc gccgggccga caaggcgggc gcgatcctcg cgctggtcgg ggtggtgaac
     2461 attccgatca tctatttttc ggtgaagtgg tggaacaccc tgcaccaggg ggcgtcggtc
     2521 agcctgaccc gagcgccgag catggcgacg atgatgctct gggggatgct gctatgcacc
     2581 ctggcgtgct ggatgtacag cattgccgtc gccctgatgc gcgtgcgcat gatcatgctc
     2641 gaacgtgagc ggcacaccga ctgggtacac gccctgctca tcgacccgga gtaaacgcat
     2701 gcactggaac agccttgccg agtttctggc catgggtcgc cacggcctct acgtctgggg
     2761 atcggtcatc gtgatggcac tgctgatgct ggcggaaccg atgctgctgc ggcatggtcg
     2821 gaagcaactc atcgcccgcc tcaggcg
//
LOCUS       DAOSIT010000296         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30607_length_2845_cov_9.318280, whole genome shotgun sequence.
ACCESSION   DAOSIT010000296 DAOSIT010000000
VERSION     DAOSIT010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30607_length_2845_cov_9.318280"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1414)
                     /locus_tag="PLL14_07735"
     CDS             complement(<1..>1414)
                     /locus_tag="PLL14_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03857.1"
                     /translation="FLLAAADALPTRRDALFGDDEPAGEDDAPEQREPEVPASRGTDA
                     PVSRDSLFADEPPAKPTTSASPAGRDALFGDPPARPQAPPEPSSRVAGFRGFIQNVMA
                     YDWPKPQHWSEMMTRADVSAQGDFSSNVKWKLGVRVDYDATYTFTDFYPQAVADNQRF
                     NILLRENYLDISAGDWDFRLGRQQIVWGEMVGLLFGDVVSAKDMRQFILPEFEILRIP
                     QWAARAEYFKDDFHAELVWIPIASYDNIGKPGSEFYVYTLPPPPGFGTSFRNEKFPTR
                     SLANTNYGLRLSMLKDGWDVAAFAYSSMSVAPTFYREIVAGPQPTVVYQARHDRIQQF
                     GSTLAKDFGSVVLKAEAVYTRGLNYQVTNPGDADGVVPQNTLTWVLGLDFTEFADTRI
                     NTQIFQSHFFNHDPDIIQSTNEYGYSLLVNHKFSDKFEAEALWIASLNRTDWMLRPRV
                     TWNLEKNWRFALGVDIFNGSP"
     assembly_gap    1415..1514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1515..2173)
                     /locus_tag="PLL14_07740"
     CDS             complement(<1515..2173)
                     /locus_tag="PLL14_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG03858.1"
                     /translation="MSVVRIEHVFKDYLLGEQKVQALKDITLAFDPGVFLAIAGPSGS
                     GKTTLLNLIGCIDTPSSGKIYINDQDVSGRTPDQLAKLRARTIGFIFQTFNLLPVLSA
                     AENVEYPLLHRTDLSPAERQKRVAHFLDVVGLSKYARNRPNQLSGGQRQRVAIARALA
                     IKPAIVLADEPTANLDHKTGEEILLLMKKINRAYKTTFIFSTHDKRVIARADRLVRIE
                     DG"
     gene            2228..>2845
                     /locus_tag="PLL14_07745"
     CDS             2228..>2845
                     /locus_tag="PLL14_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOG03859.1"
                     /translation="MEIMTIRHSGVNTALALLAAACLACAAPGRAAEAVPQAGGEDAE
                     ARMIVEKADQVRFPADGFQVDVSITTTGSGPSTEVRKYRVLSKGNTNSVVMVTEPASE
                     RGQILLMKGRDLWVFMPDVSQPIRLSLSQRLTGQVANGDLARANFAADYNPKLLRSEK
                     IGNEDFHVLELIGVDRSVTYQRVHYWVREKDFWPHKAEFYSLSNRL"
BASE COUNT          522 a          857 c          849 g          517 t
ORIGIN      
        1 ggcgagccgt tgaaaatatc cacccccagc gcgaagcgcc agttcttctc caggttccag
       61 gtgacgcgcg ggcgcagcat ccagtcggta cgattcaggc tggcaatcca cagcgcttcc
      121 gcctcgaact tgtcgctgaa cttgtggttc accagcaggc tgtagccgta ttcgttggtc
      181 gactggatga tatccgggtc gtggttgaag aaatggctct gaaagatctg ggtattgatc
      241 cgcgtatcgg cgaactcggt gaaatcgagg ccaagcaccc aggtcagggt gttctgcggc
      301 accaccccgt cggcatcgcc gggattcgtc acctgatagt tcaggccgcg cgtatagact
      361 gcttccgcct tcaacaccac cgagccgaaa tccttggcca gagtgctgcc gaattgctgg
      421 atacggtcat gcctggcctg gtagaccacc gtcggctgcg gaccggcaac gatctcgcgg
      481 tagaaggtcg gcgcgacgct catgctgctg tacgcaaagg ccgccacgtc ccagccatcc
      541 ttcaacatcg acagacgcag gccgtagttg gtattggcca gactgcgcgt gggaaacttt
      601 tcgttgcgga aagacgtgcc gaagcccggc ggcggtggca gggtatagac gtaaaactcg
      661 gatcccggct tgccgatgtt gtcgtagctg gcgatcggta tccagaccag ttcggcgtga
      721 aagtcgtcct tgaaatactc cgctcgggca gcccactgcg gtatgcgcaa gatctcgaat
      781 tcgggcagga tgaactgccg catgtccttg gcggaaacca cgtcgccgaa cagcaggccg
      841 accatttccc cccagacgat ctgctgccga ccgagacgaa aatcccagtc accggcgctg
      901 atgtcgaggt agttttcacg caacaggata ttgaagcgct ggttgtcggc aaccgcctgc
      961 gggtagaaat cggtgaaggt gtatgtcgcg tcgtagtcga cgcgaacccc cagcttccac
     1021 ttgacgttac tgctgaagtc accctgggcg ctgacatcgg cgcgggtcat catttccgac
     1081 cagtgttgcg gcttcggcca gtcgtaagcc atcacgttct ggatgaaacc ccggaaaccg
     1141 gctacccgcg acgagggctc gggcggagcc tgcggccgcg ccggcgggtc cccgaacagc
     1201 gcgtcgcgac cggccggcga tgccgatgtc gtcggtttcg ccggaggctc gtcggcgaac
     1261 agcgagtccc gactcacggg tgcgtcggtc ccccggctcg cgggaacttc aggctcccgc
     1321 tgctcggggg cgtcgtcctc gcccgcaggc tcgtcgtcgc caaacagggc atcgcggcgc
     1381 gtcggcagcg cgtcggcggc agcgagcaga aagcnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnccatcc tcgatgcgca ccagacggtc ggccctggcg atcacccgct
     1561 tgtcgtgcgt cgaaaagatg aaggtcgtct tgtaggcccg gttgatcttc ttcatcagca
     1621 gcaggatttc ctcgccggtc ttgtgatcga ggttggccgt cggctcatcg gccaggacga
     1681 tggccggctt gatcgccaat gctcgggcga tcgccacccg ctgccgctgg ccaccgctca
     1741 actgattcgg acgattcctg gcgtatttcg acaggcccac gacatcgaga aagtgcgcca
     1801 cgcgcttctg gcgctccgca ggcgacagat ccgtgcgatg cagcagcgga tattcgacgt
     1861 tctcggcggc cgacaacacc ggcagcaagt tgaaggtctg aaagatgaaa ccgatggtgc
     1921 gcgcgcgcag tttggcgagt tggtcgggcg tgcgcccgct gacatcctga tcgttgatgt
     1981 agatcttgcc gctgctcggc gtgtcgatgc agccgatcag gttcaggagc gtcgtcttgc
     2041 cgctgcccga aggtccggcg atcgccagaa aaacgcccgg atcgaaggcc agggtaatgt
     2101 ccttgagggc ttggactttc tgctcgccga gcagataatc cttgaatacg tgttcaatgc
     2161 ggacaacgct cattcgctgg cacttcccgc caaccgcgtc gacggtcggc acaagagggt
     2221 gaaaactatg gaaatcatga ccatccgtca ttctggtgtc aacaccgcgt tggcgctcct
     2281 ggctgccgcc tgcctcgcct gcgctgcacc cggcagggcc gcggaggccg tcccgcaggc
     2341 cggcggcgaa gacgccgaag cccgcatgat cgtcgaaaaa gccgaccagg ttcgcttccc
     2401 ggcagacggc tttcaggtcg atgtcagcat taccaccacc ggcagcggac ccagtaccga
     2461 agttcgcaaa taccgcgtgc tctcgaaggg gaacaccaac agcgtggtga tggtcaccga
     2521 acccgcttcc gagcggggcc agatcctgct gatgaaaggc cgcgaccttt gggtgttcat
     2581 gcccgatgtc tcacagccga tccggctctc gctttcgcag cgactgaccg gtcaggtggc
     2641 caacggcgac ctggcgcggg ccaacttcgc cgccgactac aatccgaagc tgctgcgcag
     2701 cgaaaagata ggcaacgaag acttccacgt cctggagttg atcggcgtag accgcagcgt
     2761 cacttaccag cgggtacact actgggttcg ggaaaaggat ttctggccgc acaaggccga
     2821 gttctactcg ctctccaacc gcctg
//
LOCUS       DAOSIT010000297         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30608_length_2845_cov_8.669176, whole genome shotgun sequence.
ACCESSION   DAOSIT010000297 DAOSIT010000000
VERSION     DAOSIT010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30608_length_2845_cov_8.669176"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLL14_07750"
     CDS             complement(<1..467)
                     /locus_tag="PLL14_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOG03860.1"
                     /translation="MSRLIYSLLFYLAMPLIWLRLLWRARRQPEYLQQLGERHGFYRA
                     RSAQPLLWLHAVSVGETRAAEPLIAALLRDYPAHGVLLTHMTPTGRAAGGELLRRYPG
                     RVLQVYLPYDLPGACARFLDHFRPQIGLLMETEIWPNLIAAAGKRNLPLALINA"
     gene            complement(464..>1087)
                     /locus_tag="PLL14_07755"
     CDS             complement(464..>1087)
                     /locus_tag="PLL14_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG03861.1"
                     /translation="GYAAEAAREPLAARFYDATFVIPRNVHAVQRNRWLAAAVFEYPL
                     DLPLDYGIAAPVSVFSWLPTGPLAVLLTATSRDDKLWDERHWSAVGRALLARGLTPVL
                     PAGNALERQRAERLAAAVVGSVVAPPLRLPQVAALIARASLAIGVDTGLAHLAAALRV
                     PVLALYIATDPALTGVCGTGFVRNLGGAGTPPSVQEVLTVAAQALRA"
     assembly_gap    1088..1187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1424..2845
                     /locus_tag="PLL14_07760"
     CDS             1424..2845
                     /locus_tag="PLL14_07760"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOG03862.1"
                     /translation="MNSPVVRPVVLCGGSGTRLWPLSRTHYPKQFLCLVDEFSLLQNT
                     LRRLDGLAGLGRTIAVANENHRFLVAEQLHEIGLQADLLLEPAARNTAPAVAAAALHA
                     LGEAPGEDPPLLLVLPSDHVIAEVEVFQAAVRAAIPHALAGALVTFGVVPDRAETGYG
                     YIRRGDPVLGGYALDRFVEKPDPETAQAYLDSGQYFWNSGMFLFRADTYLEALQRFAP
                     GIRECVASAVAAARRDLDFLRLDQSAFAASPANSIDYAVMEKTEHGVVIPLDAGWNDI
                     GAWDALATIGQADANNNTQHGDVLSIDTRNCVLLSEGRLLATVGVSDLVVVATPDAVL
                     VANKAKAQEVKALVDELKRSSRAETEFRHIVHRPWGSYEGVAHGPRYQVKRILVKPGA
                     SLSLQKHHHRAEHWIVVKGTAMIVRGDDSLLLSENQSTYIPLGVVHRLENPGKIDLEI
                     VEVQSGSYLGEDDIVRLEDSYGR"
BASE COUNT          504 a          914 c          888 g          439 t
ORIGIN      
        1 gcgttgatca acgccaacgg tagattgcgc tttccggcag cggcgatcag gttaggccag
       61 atctcggttt ccatcagcag cccgatctgc gggcggaagt gatcgaggaa gcgggcacag
      121 gcacccggca gatcgtaggg caggtacacc tgcagcacgc gccctgggta ccgtcgcagc
      181 agttccccac ccgccgctcg accggtcggg gtcatgtgcg tcaacaacac accgtgcgcg
      241 gggtagtcgc gcaacagcgc ggcgatcagc ggttcggcgg ccctcgtctc gcccaccgaa
      301 accgcgtgca gccagagcag tggctgtgcg gaacgcgctc gataaaaacc gtgccgctcg
      361 ccaagctgtt gcagatactc gggctgccgg cgcgcccgcc acaacagccg cagccagatc
      421 aagggcatcg ccagatagaa cagcagcgaa tagatcagac ggctcatgcg cgcagggcct
      481 gcgcggcgac cgtcaggacc tcctgcaccg acggtggtgt acccgcacct ccgagattcc
      541 gcacgaagcc ggtaccacaa acgccggtca atgccggatc cgtcgcgatg tagagcgcga
      601 gcaccggcac gcgcagggca gcggcgaggt gcgccaggcc ggtatcgacg ccgatcgcca
      661 ggctggcgcg tgcgatcagt gccgccacct gcggcaggcg caggggcggc gccaccaccg
      721 atccgaccac cgcagcggca aggcgctcgg cgcgctgccg ttcgagcgcg ttaccagcag
      781 gcagaacggg ggtgagtccg cgcgccagga gcgcccgccc gaccgcactc caatgccgtt
      841 catcccacaa tttgtcgtcg cgactggtcg cagtcagcag caccgccaga ggtcccgtgg
      901 gtagccagga gaacacactc accggcgcag cgatcccgta atccagtggc aggtcgagcg
      961 ggtactcgaa aactgcggcg gccagccagc ggttgcgctg tacggcgtgc acattgcgcg
     1021 gaatcacgaa ggtggcgtcg tagaaacgcg cggcgagcgg ttcacgcgcc gcttcggcgg
     1081 cgtaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct ccggcgcagg
     1201 tcgctgacca ccgggaggtt gtgaattacg tcaccgagcg aagaggtctt gaccagtagg
     1261 atgcgcatcg gtgagccggc ggtggggacg atcggcggga gccgcgaagt gcgcattata
     1321 gaagatgcct gcgcgcctca ggccggctcg cagcccgacg aagcgctata atgccgcctc
     1381 ctgcaaacct gcggagactc tgccccctct ggagacacgc tcaatgaatt cccctgtcgt
     1441 tcgcccggta gtcctgtgtg gcggttccgg aacccgtttg tggccccttt cgcgaacgca
     1501 ctatccgaag cagttcctct gcctggtcga cgagttttcg ctgctgcaga acaccctgcg
     1561 tcgactcgac ggactcgcgg ggctgggccg gacgattgcc gtggccaacg agaaccaccg
     1621 cttcctggtc gccgagcagc tccacgagat cgggctgcag gccgatctcc tgcttgaacc
     1681 ggcagcgcgc aacacggcgc cggccgtcgc cgccgccgca ttgcatgcgc tcggagaagc
     1741 gcctggagaa gacccgcccc tgctgctggt acttccgtcg gaccatgtga tcgccgaggt
     1801 ggaggttttt caggctgcag tcagggcggc gattccgcat gccctcgccg gtgctctggt
     1861 cacctttggc gtcgtccccg accgtgccga aaccggctac ggctatattc gccgcggcga
     1921 tccggtgctc ggcggctacg ctctcgaccg tttcgtcgag aaacccgacc ccgaaaccgc
     1981 gcaggcctac ctggattccg gccaatactt ctggaacagc ggcatgttcc tgttccgtgc
     2041 cgacacctac cttgaggccc tgcagcgctt tgcgccgggt attcgtgagt gcgttgcctc
     2101 ggctgtggct gccgcgcgtc gcgatctcga cttcctgcgc ctcgatcagt cagccttcgc
     2161 ggccagcccg gcaaactcga tcgactacgc ggtcatggaa aagaccgagc acggggtggt
     2221 gattccgctc gatgccggct ggaacgacat cggcgcctgg gatgcgctgg caacgatcgg
     2281 ccaggccgat gccaacaaca acacgcaaca cggcgatgtg ctcagcatcg atacccgcaa
     2341 ctgtgtgttg ctgtccgaag gccgtttgct cgccacggtt ggcgtcagcg acctggtcgt
     2401 ggtggcgaca ccggacgccg tgctggtggc caacaaggcg aaagcacagg aggtcaaggc
     2461 gctggtcgac gaattgaagc gctcgtcccg cgccgagacc gaattccggc acatcgtaca
     2521 tcgcccctgg ggcagctacg aaggggtggc gcacggtccg cgttaccagg tcaaacgcat
     2581 cctggtcaag ccgggggcca gcctgagtct gcagaagcac caccatcgcg ccgaacactg
     2641 gatcgtcgtc aagggaacgg caatgatcgt ccgcggcgac gactcgctgc tgttgtcgga
     2701 gaaccaatcg acgtatatcc ccctcggcgt ggttcatcgc ctggaaaacc cgggcaagat
     2761 cgacctcgaa atcgtcgagg tccagtccgg cagctatctg ggggaagacg acatcgtccg
     2821 cctcgaagac agctacggcc gctag
//
LOCUS       DAOSIT010000298         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30695_length_2840_cov_10.523878, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000298 DAOSIT010000000
VERSION     DAOSIT010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30695_length_2840_cov_10.523878"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLL14_07765"
     CDS             complement(<1..545)
                     /locus_tag="PLL14_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03863.1"
                     /translation="MRLSTFFEELKSAYEAELDDLTTDSAGHDVLAKRLQAKRGQVSQ
                     LLPMIECNPEMVAVAFHGGMRFDSPEVMRDLAAQEPEDLPTWTELLASVHLEPWAETL
                     ANIVLKDPNGEQFLVVTACLEYLLGRSAIPSESGEGPSRGSGGDGGGDDDDGRAESDD
                     DVDLEEAGADWLSEQGFDRRDR"
     gene            complement(542..820)
                     /locus_tag="PLL14_07770"
     CDS             complement(542..820)
                     /locus_tag="PLL14_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOG03864.1"
                     /translation="MKDHYAILGIASDASSDEVRSAYRRKALQFHPDRNTAPEATDKF
                     RDVQEAYETLSDVARRYAYDENRRRNLLEKPLETAREIWKHYLEGVLQ"
     gene            complement(941..1780)
                     /gene="metF"
                     /locus_tag="PLL14_07775"
     CDS             complement(941..1780)
                     /gene="metF"
                     /locus_tag="PLL14_07775"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG03865.1"
                     /translation="MTPDLRTFSLELFPPQTPEGAEKLRAARARMALLKPSFFSVTFG
                     AGGSTRDRTLDTVLEIQSEGHAAAPHLSCIGSTRESIRSILEQYRAHGIRQLVALRGD
                     LPSGMARPGAFRYANELVEFIRAETGDWFHIEVAAYPEYHPQARSPREDLLAFKRKLD
                     AGANSAITQYFYNFDAYAHFVDESRAIGIDVPIVPGIMPISSYSSLARFSDNCGAEIP
                     RWIRRRLESYGDDTASIQAFGLDVVTHLCEQLLASGVPGLHFYTLNQVLLTSTLWQRL
                     GLA"
     gene            complement(1788..2141)
                     /locus_tag="PLL14_07780"
     CDS             complement(1788..2141)
                     /locus_tag="PLL14_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOG03866.1"
                     /translation="MRLILLWLLNALALLAVAYLMPSIQVASFGSAMVAALVLGLVNA
                     VLRPLLLLLTLPVTLLTLGLFLFVLNGLMFWLAGSLLEGFVVGGFWPGVFGAILYSIF
                     SSLLSSLLPTGKRTP"
     gene            complement(2166..>2840)
                     /locus_tag="PLL14_07785"
     CDS             complement(2166..>2840)
                     /locus_tag="PLL14_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOG03867.1"
                     /translation="TDVMIAGKVALIAGYGDVGKGSAQAMRALSAQVWVTEIDPICAL
                     QAAMEGYRVVTMDYAADKADIFVTCTGNYHVITHAHMQKMKDQAIVCNIGHFDNEIDV
                     ASVEVYPWEEIKPQVDHVIFPDGKRIILLAKGRLVNLGCGTGHPSYVMSSSFANQTIA
                     QIELFTRNADYPIGVYTLPKHLDEKVARLQLKKLNAQLSELTDQQAAYIGVAKEGPYK
                     SDHYRY"
BASE COUNT          530 a          872 c          920 g          518 t
ORIGIN      
        1 cgatccctgc gatcgaagcc ctgctcggaa agccagtcgg caccggcctc ttcgaggtcg
       61 acatcgtcgt cggactccgc gcgcccgtcg tcatcatcgc cgccgccgtc acccccgctg
      121 ccccgcgacg gtccttcgcc ggactccgaa gggatcgccg aacggccgag caggtactcc
      181 aggcacgcgg tcacgaccag gaactgctcg ccattcggat ccttgaggac aatgttggcc
      241 agggtttcgg cccacggctc caggtgtacc gacgccagca attccgtcca ggtgggcagg
      301 tcttctggtt cctgcgcggc gaggtcgcgc atcacttcgg gactgtcgaa acgcatcccg
      361 ccatggaagg cgacggcgac catctccggg ttgcactcga tcatcggcag caactgggac
      421 acctggcccc gctttgcctg cagccgtttc gccaggacat cgtgtcccgc cgaatcggtc
      481 gtcaggtcgt cgagttcggc ctcataggcg gatttcagtt cttcaaaaaa tgtcgagagt
      541 ctcactgcaa tactccttca agatagtgct tccagatctc ccgggccgtt tcgagcggct
      601 tctcgagcag attgcggcgt cgattctcat cataggcgta gcgtcgcgcg acgtccgaaa
      661 gcgtttcgta ggcttcctgg acatcgcgga atttgtccgt cgcttccggg gccgtgttcc
      721 ggtccgggtg aaactgcaag gccttcctgc gataggccga cctgacctcg tcgcttgacg
      781 cgtcgctggc gatacccagt atggcgtaat ggtccttcat gcaccctcaa caagtcgctg
      841 ggcgtgccgc ccgatggccc attctagcag cctcggcgca cccgcatacg ggcagggccg
      901 ctcgacggct gcccgcatgc ggcgcttgtg ccgttgccga tcaggcaagc cccaggcgct
      961 gccacagggt gctggtcagc agcacctgat tgagggtgta gaagtgcagg ccgggcaccc
     1021 cactggccag cagttgttca cagaggtgcg tgacgacatc cagtccgaag gcctggatcg
     1081 atgccgtatc gtcaccgtag ctttcgagcc tgcgccggat ccagcgcgga atctcggcgc
     1141 cgcagttgtc ggaaaagcgc gccagactgc tgtagctgga aatcggcatg atgccgggaa
     1201 cgatcgggac gtcgatgccg atcgcccggg attcgtcgac gaagtgcgcg taggcatcga
     1261 aattgtagaa atactgggtg atcgccgaat tggcgccggc atcgagcttg cgcttgaagg
     1321 ccagcaggtc ttcgcgcggg ctcctggcct gcgggtgata ctccgggtac gcggcgactt
     1381 cgatgtggaa ccagtcgccg gtttcggcac ggatgaattc gacgagttcg ttggcgtagc
     1441 gaaacgcgcc gggccgggcc atccccgacg gcaggtcgcc gcgcagcgcc acgagctgcc
     1501 ggataccgtg cgcccggtac tgctccagga tgctgcgaat gctctcgcgc gtcgaaccga
     1561 tgcacgacag gtggggcgcc gcggcgtggc cttcgctctg gatttcgagc acggtatcga
     1621 gggtgcggtc gcgcgtcgaa ccgccggcac caaaagtgac ggagaagaag ctgggtttga
     1681 gcagcgccat gcgcgcgcgc gcggcgcgca gcttctccgc accctcgggc gtttgtgggg
     1741 ggaagagctc aagactgaag gtgcgaaggt caggggtcat gacgagacta tggcgtacgt
     1801 tttcccgtcg gcaggagcga cgaaagcagc gacgagaaaa tgctgtagag gatggcgccg
     1861 aacactcccg gccagaagcc gccgacgacg aagccttcca gcagcgaacc cgccagccag
     1921 aacatcaggc cattcaggac gaagagaaag agccccagcg tcaacagcgt gaccggcagc
     1981 gtcagcagga gcaacagggg gcgcaagacg gcattcacca gtccgagcac cagagccgcg
     2041 accatcgccg acccgaaaga cgccacctgt atcgatggca tcagataggc aacggcgagc
     2101 agcgcgaggg cgttgagcaa ccagagcagg atcagtcgca tggcggtccc ctgtcgaggt
     2161 aggtatcagt agcgataatg gtcggacttg taggggcctt ccttggcgac accgatgtac
     2221 gccgcctgct gatcggtcag ttcggaaagc tgcgcattga gtttcttgag ttgcaggcgg
     2281 gcgaccttct cgtcgaggtg tttgggcagc gtatagacgc cgatcgggta gtcggcattg
     2341 cgcgtgaaca actcgatctg ggcgatcgtc tggttggcga acgacgaact catcacgtag
     2401 gacggatggc cggtaccgca accgagattg accaggcgac ccttggccag caggatgatg
     2461 cgcttgccat ccgggaagat gacatggtcg acctgcggct tgatctcttc ccagggatag
     2521 acctcgaccg aggcgacgtc gatttcgttg tcgaagtggc cgatgttgca gacgatcgcc
     2581 tgatccttca tcttctgcat gtgcgcatgc gtgatgacgt ggtagttgcc ggtgcaggtg
     2641 acgaagatgt cggccttgtc ggcggcgtag tccatggtca ccacgcggta accttccatg
     2701 gccgcctgca gcgcacagat cggatcgatt tcggtgaccc acacctgcgc cgacaaggcg
     2761 cgcatggcct gcgccgagcc cttgccgacg tcgccatagc cggcgatcaa ggcgaccttg
     2821 ccggcgatca tcacgtcggt
//
LOCUS       DAOSIT010000299         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30710_length_2839_cov_8.299569, whole genome shotgun sequence.
ACCESSION   DAOSIT010000299 DAOSIT010000000
VERSION     DAOSIT010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30710_length_2839_cov_8.299569"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1005)
                     /locus_tag="PLL14_07790"
     CDS             complement(85..1005)
                     /locus_tag="PLL14_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03868.1"
                     /translation="MRDHTALEVARRTVLPIALISPALLLLLYYSVRRALKPLDELAA
                     DVASRSPENLRPLAQNAAPLEARPLVSALNRLFGRLDDTLANERRFTADAAHELRTPL
                     AVVRIQAQVAQLSRNTEDRQHALQQIVLGADRATRVLEQLLRLARLDPLAGLPHPAEV
                     DLAGLAERVLSDARHATADRCIVLERSDDPLLLSGDAELLAIALRNLLDNALRYTPPA
                     TTIRVIAARQDGELTLGVADDGPGVPPAELPRLVERFYRGSAARAEGSGLGLAIAQRV
                     AELHTARFELENLPGGGFAARLRWPRPPTA"
     gene            complement(1059..1460)
                     /locus_tag="PLL14_07795"
     CDS             complement(1059..1460)
                     /locus_tag="PLL14_07795"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOG03869.1"
                     /translation="MPGTVTPTHSQSLRWRLLRAVFATALLVWGLNAILSYTQARHEA
                     EELMDGHLAQAARLLLALVRDNESHLEDLATRLASVRSQEKHRYESPLEFQVGRADGS
                     LLCGRHRRRQNRWPRRPATTTYPTPATPGES"
     gene            complement(1460..2122)
                     /locus_tag="PLL14_07800"
     CDS             complement(1460..2122)
                     /locus_tag="PLL14_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03870.1"
                     /translation="MRILLVEDDDLLGDGIRAGLKLADYAVDWVRDGEAARLALLDHA
                     YQACVLDLGLPKRDGLWLLKDLRERGNPLPVLILTARDSSADKIAGLDAGADDYLTKP
                     FDLFELQARLRALIRRAGGNATPRLEHAGVVLEPASKRVSRDGQPVTLSAREFALLRD
                     LLSHKNHIRTRAQLEESLYAWGEETGSNTVEVYIHHLRRKLGAQFIRTVRGLGYRLGD
                     PG"
     gene            complement(2149..2772)
                     /locus_tag="PLL14_07805"
     CDS             complement(2149..2772)
                     /locus_tag="PLL14_07805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG03871.1"
                     /translation="MPVELQHARLHLASRSPRRRELLTQIGVAFDSIVFRSSPRDDAE
                     LDETPLPDEDPVHYVQRIAGNKAAHGCRIVDWRRLLPQPVLAADTTLELAGRLIGKPR
                     DASDAESILRRLSGNTHRVLTAVAVGSGERLELAVSISEVRFRPLEAHEITRYVASGE
                     PMDKAGAYAIQGRAGMFVEYLAGSYSGVMGLPLCETAQLLKRFGYRL"
BASE COUNT          429 a          905 c         1026 g          479 t
ORIGIN      
        1 gctgagcctc atcgccgtac tcctggccgc cctgctgctc agccagtatc tgtgatcatc
       61 ctggccatgc gcggaggcac aggctcaggc tgttggcggt cgcggccagc gcaggcgcgc
      121 cgcgaatccc ccgccgggca ggttttccag ttcgaaacgg gcggtgtgca gttcggcgac
      181 acgctgcgcg atggccagtc cgaggccgct gccttcagct cgcgccgcgc tgcctcgata
      241 aaagcgttcg accagccgcg gcagttccgc gggcggaacg ccggggccgt cgtcggcgac
      301 gccgagcgtc aactcgccgt cctgtcgcgc ggcgataacg cggatcgtcg tcgccggcgg
      361 ggtgtagcgc agcgcgttgt cgagcagatt gcgcagcgcg atggccagca attcggcgtc
      421 accgctgagc agcaaggggt cgtcgctacg ttccagaacg atgcagcggt cagcggtggc
      481 gtggcgggca tcgctcagca cccgctctgc cagcccggca aggtcgactt cggcgggatg
      541 aggcagcccg gccagcgggt cgaggcgcgc cagacgcagc agttgttcga gcacacgggt
      601 ggcacggtcg gcgccaagca cgatctgttg caacgcgtgc tgtcggtctt cggtgttgcg
      661 actgagttgc gcgacctggg cctggatcct gaccaccgcc agcggcgtac gcagctcgtg
      721 cgcggcgtcg gccgtgaatc gcctttcgtt ggccagggtg tcgtcgagcc ggccgaagag
      781 gcggttgagc gccgaaacca gcggccgggc ttcgaggggg gccgcgttct gggccagcgg
      841 ccggaggttt tccggcgagc gcgacgcgac gtcggcggcc agctcatcga gcggcttcag
      901 cgcgcgccgc accgagtagt agagcagcaa aagcagcgcc ggcgagatca gcgcgatcgg
      961 caacaccgtc cggcgggcga cctcgagcgc cgtgtggtcc cgcaagtcga tcgattgtgc
     1021 gacctgtacc cggtagtcgc cagaggtcga ggcgacggtc aggactcgcc aggggtggcc
     1081 ggcgtgggat acgtcgtcgt agccgggcgc cgcggccagc gattttgccg gcgcctgtgc
     1141 cgaccgcaga gcagtgagcc gtcggcacgc ccgacctgaa attcgagcgg tgactcgtaa
     1201 cgatgctttt cctggctgcg gaccgacgcc aggcgcgttg ccaggtcttc caggtggctc
     1261 tcgttgtcgc gcaccagcgc cagcaacagg cgggcagctt gcgccagatg gccgtccatc
     1321 agttcttcgg cttcgtgccg ggcctgggtg tagctgagga tcgcgttcag gccccagacc
     1381 agtagcgcgg tggcgaagac ggcgcgcagc agccgccagc gcagcgactg gctgtgcgtg
     1441 ggtgttaccg tcccgggcat cagccagggt cgcccaggcg gtagcccagg ccgcggacgg
     1501 tgcggatgaa ctgggcgccg agttttctgc gcaggtggtg gatatatacc tcgacagtgt
     1561 tgctgccggt ttcctcgccc caggcataca gactttcctc gagttgggcg cgcgtgcgga
     1621 tatggttctt gtggctgagc aggtcgcgca gcagggcgaa ttcgcgcgcc gagagggtga
     1681 ccggctggcc gtcccggctg acccgcttgc tggccggttc gagcaccacg ccggcgtgct
     1741 ccaggcgcgg cgtggcgttg ccgccggcgc ggcgaatcag ggcgcgcagg cgtgcctgca
     1801 gttcgaacag gtcgaagggt ttggtgaggt agtcgtcggc gccggcgtcg agcccggcga
     1861 tcttgtcggc gctgctgtcg cgcgccgtga gaatcagtac cggcaacgga ttgccgcgtt
     1921 cgcgcaggtc cttcagcagc cacagaccat cgcgcttcgg caggccgagg tcgagaacgc
     1981 aggcctgata ggcgtggtcg agcagtgcca ggcgtgcggc ttctccgtca cgtacccagt
     2041 cgacggcgta gtcggccagc ttcaggccgg cacgtatgcc gtcaccaagc aggtcatcgt
     2101 cttccaccag cagtatccgc acggatcgtc tcccttccga acgaggtgtt acagacgata
     2161 tccgaagcgc ttcaacaact gtgccgtttc gcacagcggc aggcccatga caccgctgta
     2221 gctgccggcg agatactcga cgaacatccc ggcgcgcccc tgaatggcgt aggcgccggc
     2281 cttgtccatc ggttcgccgc tggcaacgta gcgggtgatc tcgtgcgctt cgagggggcg
     2341 aaaacgcacc tcgctgatcg atacggcgag ttcgaggcgt tcgccgctac ccacggcgac
     2401 ggcggtaagc acccggtgcg tgttgccgga caagcgtcgc aggatggatt cggcatcgct
     2461 ggcgtcgcgg ggcttgccga tcagccgacc ggccagttcg agagtggtgt cggcagccag
     2521 caccggttgc ggcagcagtc ttcgccagtc gacgatgcgg cagccgtgtg ccgccttgtt
     2581 gcccgcaatg cgttgcacgt agtgcaccgg gtcctcgtcg ggcagtggcg tttcgtcgag
     2641 ttcggcgtcg tcgcgcggcg aactgcggaa gacgatgctg tcgaaagcga cgccgatctg
     2701 ggtcagcagt tcgcgccgac gtgggctgcg cgaggccaga tgcaggcgcg cgtgctgcag
     2761 ttcgaccggc atcagccctc ccggtggtaa gggtgctggc ggacgatgct gatcgcgcga
     2821 tacaactgct cgcagagga
//
LOCUS       DAOSIT010000300         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_30746_length_2836_cov_9.472851, whole genome shotgun sequence.
ACCESSION   DAOSIT010000300 DAOSIT010000000
VERSION     DAOSIT010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30746_length_2836_cov_9.472851"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLL14_07810"
     CDS             <1..453
                     /locus_tag="PLL14_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG03872.1"
                     /translation="TWQVSNIHGGTGAGNVIPGSVVIDFNFRFGTASTPEQLQTRMRA
                     LLDRHGLDHDLVWTLGARPFLTGRGALIDAALLAIRNVTGHQAELSTSGGTSDGRFIA
                     EICPQVIEIGPVNASIHKVDECVEEAAPAQLAAIYRHILEQLLPAADV"
     gene            446..1339
                     /gene="prmB"
                     /locus_tag="PLL14_07815"
     CDS             446..1339
                     /gene="prmB"
                     /locus_tag="PLL14_07815"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG03873.1"
                     /translation="MFEQAQTELATLRDLLRFAVSRFNAAGLCFGHGADNAWDEAVYL
                     LLHSLHLPRDRLEPFLEARLTSAERANVLRIIQRRIDERLPAAYLTHEAWLGEYRFYV
                     DQRVIVPRSFIAELLREQLAPWIDDPDAVQAVLDLCTGSGCLAIVAAHAFPDARIDAV
                     DLSPAALDVARRNVADYDLAARIRLAEGNLFAAIGKQRYDLIIANPPYVNAESMATLP
                     AEYRREPELALASGEDGLDLTRAILAGAGKHLKPHGLLVVEIGHNREQLEAAFPATPF
                     TWLDTSAGDQYVFLLHRDELP"
     gene            1336..1962
                     /locus_tag="PLL14_07820"
     CDS             1336..1962
                     /locus_tag="PLL14_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOG03874.1"
                     /translation="MSLLRLLLAILLLAGCHDAPRLAALPAGSQVLAFGDSITFGSGA
                     GAGEDYPSQLAASSGWEVHNAGVPGDTAENAGQRIRQVLAETRPVLVIVEIGGNDFLR
                     RRPEAAVKEDLRALITTIKAAGVQVVLVAVPKLSLIGAVTGKLPDAAIYADLANEQKL
                     PLVDGVLASVLSDPALKADPIHPNATGYRKLAEGISDRLIRAGLLNKP"
     gene            2022..>2836
                     /gene="pepN"
                     /locus_tag="PLL14_07825"
     CDS             2022..>2836
                     /gene="pepN"
                     /locus_tag="PLL14_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOG03875.1"
                     /translation="MQTETPQKIFRLDYTPPPFLVEHVQLDVQIHRGEVLVRCELRLH
                     RNPAVPPGQPLQLDGHELETLTVAIDGQALGKERYTCSDSTLTIDSPPESLTLQTLVR
                     IDPDHNTSLSGLYRSKDGYFTQCEAQGFRRITWFPDRPDIMSRYVVTLHADKASLPVL
                     LANGNPVGSGDEADGRHWARWEDPFPKPCYLFALVAARLDVLRDRITTASGRGVALSV
                     FVEHGKLDQCAHAMDALQRAMRWDEDVFGLECDLDHYMIVAVGDFNMGAMENKG"
BASE COUNT          498 a         1032 c          863 g          443 t
ORIGIN      
        1 acctggcagg tctcgaacat ccacggcggc accggtgccg gcaacgtcat ccccggcagc
       61 gtggtcatcg acttcaactt ccgtttcggc accgccagca ccccggagca gttgcagacc
      121 cggatgcgcg cgctgctcga tcggcatgga ctggaccatg atctcgtctg gacgctcggc
      181 gccaggccct tcctgaccgg tcgcggagcg ctgatcgacg cggcgctgct ggcaatccgc
      241 aacgtgaccg gccaccaggc cgaactatcg accagcggcg gcacctcgga cggacgcttc
      301 atcgccgaaa tctgcccgca ggtgatcgag atcggcccgg tcaacgccag catccacaaa
      361 gtcgatgaat gcgtcgaaga agccgcgccg gcacaactgg cggccatcta tcgccatatt
      421 ctcgaacagt tgctgccggc cgccgatgtt tgaacaggcc cagaccgagt tggcgacgct
      481 gcgcgacctg ttgcgcttcg ccgtcagccg cttcaacgcc gccggactgt gcttcgggca
      541 cggtgccgac aacgcctggg acgaagccgt gtatctgctg ttgcacagcc tgcacctgcc
      601 ccgcgatcgc ctggaaccgt ttctcgaagc gcgcctgacc agcgcggaac gggcgaacgt
      661 gttgcgcatc atccagcgac gcatcgacga acgactgccg gcggcgtacc tgacccacga
      721 ggcctggctc ggcgagtacc gcttctatgt cgaccaacgc gtcatcgtcc cgcgctcgtt
      781 catcgccgaa ttgctgcgcg aacaactggc gccgtggatc gacgatcctg acgcggtgca
      841 ggccgtgctc gatctctgta ccggttcggg ctgcctggcg atcgtcgccg cgcacgcctt
      901 ccccgatgcc cgcatcgacg ccgtcgacct ctcgccggcg gcgctcgacg tcgcccggcg
      961 caacgtcgcc gattacgacc tcgcggcgcg catccgcctc gccgagggca acctgttcgc
     1021 cgcgatcggc aagcagcgct acgacctgat catcgccaat ccgccgtacg tcaatgccga
     1081 atcgatggcc accctgcccg ccgaataccg gcgcgaaccc gaactcgcgc tggccagcgg
     1141 cgaggatggt ctcgacctga cgcgcgccat cctcgccggt gccggcaaac acctcaagcc
     1201 gcacggcctg ctggtggtcg agatcggaca caaccgcgag cagctcgaag cggcgttccc
     1261 ggccaccccc ttcacctggc tcgacaccag cgccggcgac cagtatgtct tcctcctgca
     1321 tcgcgacgag ttgccgtgag cctgctgcgc ctgctgctgg ccatcctgct gctggcaggc
     1381 tgccacgacg cgccacggct ggcggcactg cccgccggca gccaggtcct cgctttcggc
     1441 gacagcatta ccttcggtag cggcgccggc gcgggcgagg actacccgag tcaattggcc
     1501 gcgagcagcg gttgggaagt gcataacgcc ggcgtgccgg gtgacaccgc tgaaaacgcc
     1561 ggccagcgca ttcggcaggt gctggccgag acgcgtccgg tactggtgat cgtcgagatc
     1621 ggcggcaacg atttcctgcg caggcgtccc gaagcggcgg tcaaggaaga tctgcgcgcg
     1681 ctgatcacga ccatcaaggc ggcaggagtg caggtcgtgc tggtcgcggt accgaagctg
     1741 tcgctgatcg gcgcggtcac cggcaaactc cccgacgcgg cgatctacgc cgacctggcc
     1801 aacgaacaaa aattgccgct ggtcgacggc gtcctggcgt cggtcctctc cgacccggcg
     1861 ctgaaagccg accccatcca ccccaacgcc acgggttacc gcaagctcgc ggaaggcatt
     1921 tcagaccgcc tgatccgcgc cggtttgctg aacaagccct gagcctggcc ccacgccggc
     1981 gcgcccgcag atcccaggcg ccgggcagac acggagaacc catgcaaacc gaaaccccgc
     2041 aaaagatctt tcgcctggac tacaccccac cacccttcct ggtcgagcac gtgcaactcg
     2101 acgtgcagat ccaccgcggc gaagtcctgg tccgctgtga gttgcgcctg caccgcaatc
     2161 ccgccgtacc acccggacag cccctgcaac tcgacggcca tgagctcgaa acgctcacgg
     2221 tggcgatcga tggccaggcg ctcggcaagg agcgctacac ctgcagcgat tccacgctga
     2281 ccatcgacag cccacccgag tcgctgacgc tgcagacgct ggtacgcatc gatccggacc
     2341 acaacaccag cctgtcggga ctctatcgct cgaaggacgg ctatttcacg cagtgcgagg
     2401 cacagggatt tcgtcgcatc acctggttcc ccgaccgccc ggacatcatg tcgcgctatg
     2461 tcgtgactct gcacgccgac aaggccagcc tgccggtgct gctggccaac ggcaaccccg
     2521 tcggcagcgg cgacgaggcc gacggtcgcc actgggcccg ctgggaagac ccctttccca
     2581 agccctgtta tcttttcgcg ctggtcgccg cgcgcctcga cgtgctgcgc gaccggatca
     2641 ccaccgccag cggccgcggc gttgcgctct cggtcttcgt cgaacatggc aagctcgacc
     2701 agtgcgcgca cgcgatggac gccctgcaac gcgcgatgcg ctgggacgag gacgttttcg
     2761 gcctcgaatg cgacctcgac cactacatga tcgtcgccgt cggcgacttc aacatgggcg
     2821 cgatggaaaa caaggg
//
LOCUS       DAOSIT010000301         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31050_length_2814_cov_8.563248, whole genome shotgun sequence.
ACCESSION   DAOSIT010000301 DAOSIT010000000
VERSION     DAOSIT010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31050_length_2814_cov_8.563248"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="tsf"
                     /locus_tag="PLL14_07830"
     CDS             <1..866
                     /gene="tsf"
                     /locus_tag="PLL14_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG03876.1"
                     /translation="LRERTDAPMMECKRALTESGGDLLKAEEILRVKLGSKASKAASR
                     ITAEGVVAIHRSSDDKLGAIVEINCETDFVGKNQEFLSLARDCAVLVAGYDPVDVAAL
                     SALPMGEGSVESTRTALVGKIGENMSIRRFARIEAKGRLASYVHGGAKIGVLVDYIGG
                     DDQLGKDLAMHIAAAKPRALDPTGVAVELIESERRIAIEKAREGGKPEAMIDKIAEGS
                     VQKFLNEVTLLGQVFVKDESHKQTIAQLLKAKEASVVSFTLFVVGEGIAKRANDFAAE
                     VAAQAAAAAQK"
     gene            917..1639
                     /gene="pyrH"
                     /locus_tag="PLL14_07835"
     CDS             917..1639
                     /gene="pyrH"
                     /locus_tag="PLL14_07835"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG03877.1"
                     /translation="MSTNSPKYKRILLKLSGEALMGSDAYGINRKTISGIVAEIKVIV
                     DMGVQVGVVIGGGNIFRGVAPAARGMDRAQADYMGMLATVMNGLALHDAMRVAGMVSR
                     VQSALSIEQVVEPYVRAKAIRYLEQGRTVIFSGGTGNPFFTTDTAAALRGAEIGAEIV
                     LKATKVDGIYSADPKKDPQATRYERISFDDAIAQNLAVMDATAFALCRDQKLPINVFS
                     IFKQGALRRVTMGENEGTLVYC"
     gene            1651..2211
                     /gene="frr"
                     /locus_tag="PLL14_07840"
     CDS             1651..2211
                     /gene="frr"
                     /locus_tag="PLL14_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG03878.1"
                     /translation="MSIADVKKAAEHKMHRSLEALKTDLAKVRTGRAHTGLLDHVQVE
                     YYGSPVPINTVANVTLIDARTLGVQAWEKGMTAKVEKAIRDSDLGLNPAAQGDLIRVP
                     MPALTEERRRDLIKVVKGEGEGAKVAVRNLRRDAIATLKEMLKNKECSEDDEHRAQDE
                     IQKLTDRYVAEIEKQLSQKETELMAI"
     gene            2235..>2814
                     /gene="uppS"
                     /locus_tag="PLL14_07845"
     CDS             2235..>2814
                     /gene="uppS"
                     /locus_tag="PLL14_07845"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOG03879.1"
                     /translation="MVRFASSTRDVPNTSAVPRHVAIIMDGNGRWAKKRLLPRVAGHH
                     RGVDTVRATVKTCLERGIEYLTLFAFSSENWRRPEEEVSLLMQLFVRVLKEEVKKLDR
                     NGVRLRVIGDLSRFDPDLQALIAASEERTAGNDRLVLTIAANYGGRWDILQAVKRMLN
                     SDQKARAEWSESDLAPHLAMSYAPEPDLFIRTG"
BASE COUNT          602 a          766 c          920 g          526 t
ORIGIN      
        1 agcttcggga aagaaccgac gcaccgatga tggagtgcaa gagggcgctg acggaatccg
       61 gtggcgacct gctcaaggcc gaggaaattc tgcgcgtcaa gctcggttcc aaagccagca
      121 aggcggcgag tcgtatcacc gccgagggcg tcgtggccat ccacagatcg agtgacgaca
      181 aacttggtgc gatcgtcgaa atcaactgcg aaactgactt tgtcggcaag aaccaggagt
      241 ttctgagcct ggcgcgtgat tgcgcggttt tggtcgccgg gtacgaccca gtcgacgtcg
      301 ctgccttgtc ggcgttgccg atgggtgaag gtagtgtcga gtcgacacgc acggcgctgg
      361 tcggcaagat tggcgagaac atgtcgatcc gtcgtttcgc gcgcatcgag gccaagggaa
      421 ggctcgcttc ctacgtgcat ggtggggcca agatcggtgt tctggtggat tacattggcg
      481 gcgacgatca actcggcaag gatctggcca tgcacattgc ggctgccaag ccgcgggcac
      541 tggatccgac cggtgtcgcc gtcgaactga tcgagagcga acgccgcatt gccatcgaga
      601 aggcccgtga aggtggcaag ccggaggcga tgatcgacaa gattgccgaa ggctccgtac
      661 agaagtttct caatgaggtc acgctcctcg gtcaggtgtt tgtgaaggac gaaagccaca
      721 aacaaacaat tgcccagttg ctcaaggcca aagaggcttc ggtggtgagt ttcaccctgt
      781 tcgtggttgg tgaaggcatc gccaagaggg ccaacgattt tgccgccgaa gtggccgcac
      841 aagccgctgc cgccgcgcag aagtaaccgc tgggctgact gaccgatcac gcgcttaccg
      901 aaggagaccg acgccgatgt ccaccaactc gccgaagtac aaacgcatcc tgctgaaact
      961 ctccggtgag gcgctcatgg gtagtgatgc ctacggaatc aaccggaaaa cgatctcggg
     1021 aattgttgcc gagatcaagg tgatcgtcga catgggggta caggttggcg tggtcatcgg
     1081 cggtggcaac atttttcggg gcgttgcgcc ggcggccaga ggcatggatc gcgcccaggc
     1141 ggattacatg ggcatgctgg cgacggtgat gaatggtctg gcactgcacg acgcgatgcg
     1201 tgttgccggg atggtttcgc gcgtgcagtc ggctctgagc atcgagcagg tggtcgagcc
     1261 gtacgtccga gccaaggcca tccgctacct ggagcagggc cggacggtca tcttttccgg
     1321 tggtaccggt aaccccttct ttacgaccga taccgctgcg gctctgcggg gggcggagat
     1381 cggtgccgaa atcgtactca aggccaccaa ggtcgatggc atctattcgg ccgacccgaa
     1441 gaaggatccg caggctaccc gctacgagcg gatcagcttc gacgatgcca tcgcgcagaa
     1501 cctcgcggtc atggatgcca ctgcttttgc cttgtgccgt gaccagaagc tgccgatcaa
     1561 cgtgttctcg attttcaagc agggtgcgct gcggcgggtt acgatgggcg agaacgaagg
     1621 caccctggta tattgctgac ggagatggga atgtccatag cagacgtgaa aaaggccgcc
     1681 gagcacaaga tgcacaggtc gcttgaggca ctcaagaccg atctggcaaa ggtgcggacc
     1741 gggcgtgcgc atacgggttt gctcgaccac gtgcaggtcg agtattacgg ctcgccggtg
     1801 ccgatcaata ccgtggccaa cgtgaccttg atcgacgctc gcacgctcgg tgttcaggcc
     1861 tgggaaaagg gcatgacggc aaaggtcgag aaggccattc gcgattccga cctgggcctc
     1921 aacccggccg cacagggaga cctgatccgc gtaccgatgc ctgccctgac cgaggaacgg
     1981 cggcgcgatc tgatcaaggt cgtgaaaggt gagggcgagg gtgccaaggt tgcggttcgc
     2041 aatctgcgac gcgacgccat tgcgacgctg aaggaaatgc tcaagaacaa ggagtgctcc
     2101 gaggacgacg agcatcgcgc tcaggacgag atccagaagc ttaccgaccg ctatgtggcg
     2161 gaaatcgaga agcagctcag tcagaaggaa accgagttga tggctatcta gcgactgccg
     2221 caggagtcat cagaatggtg cgttttgcca gttcgactcg cgatgtgccg aatacgtcgg
     2281 cggttcctcg ccatgtggcg atcatcatgg acggcaatgg gcgttgggcc aagaagcgac
     2341 tgttgccgcg cgtcgccggg catcatcgcg gcgttgatac ggtgcgcgca acggtgaaga
     2401 cctgccttga acggggcatc gagtatctga ccctgttcgc cttcagttca gagaactggc
     2461 gtcgccctga agaggaagtc tcgctgctga tgcaactctt cgtacgcgta ctcaaggaag
     2521 aggtcaagaa acttgatcgc aacggcgtac gcttgcgggt gattggcgac ctttcgcgct
     2581 ttgatccgga cctgcaggcg ctgattgctg cctccgagga gcgcacggcg ggtaacgacc
     2641 gactcgttct gaccatcgcc gcgaattacg gtggacgctg ggatatcctg caggcggtca
     2701 agcgcatgtt gaacagcgat cagaaggcac gcgcagaatg gagcgaaagc gatcttgcac
     2761 cacatctggc aatgagctat gcacccgagc ccgatctgtt cattcgcacc ggtg
//
LOCUS       DAOSIT010000302         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31076_length_2812_cov_9.392093, whole genome shotgun sequence.
ACCESSION   DAOSIT010000302 DAOSIT010000000
VERSION     DAOSIT010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31076_length_2812_cov_9.392093"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..575)
                     /locus_tag="PLL14_07850"
     CDS             complement(39..575)
                     /locus_tag="PLL14_07850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOG03880.1"
                     /translation="MAELVTIARPYAEAVFRIALENKALAAWSERLSLLSAIATDEQM
                     RSCIGNPELSATQKSELFKSLVGKTQEGGEANLIDVLATNERLSALPEIAGLFEALRA
                     AQEGVREATIYSAFPVDDEQRKALIEDLEARFKTRLTAEVVVDQSLIGGVKIVVGDQV
                     LDTSVRGKLESLRLALKN"
     gene            complement(580..1050)
                     /locus_tag="PLL14_07855"
     CDS             complement(580..1050)
                     /locus_tag="PLL14_07855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOG03881.1"
                     /translation="MNINATLIGEAIWFGVFIWITMKYVWPPLAKAMADRQKLIADGL
                     AAGERGKHELELAGKRSADALRDAKAKAADIISAAEKRGAQMVEEAKQVAKVEADKVV
                     ASAKAEIAQQVEQARAELRGRVADLAVAGAERILKREVDAKAHAEMLVALKQEL"
     gene            complement(1091..1348)
                     /gene="atpE"
                     /locus_tag="PLL14_07860"
     CDS             complement(1091..1348)
                     /gene="atpE"
                     /locus_tag="PLL14_07860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOG03882.1"
                     /translation="MSMLLGNTAIAVAILIGAGALGTAIGFGLLGGRFLEGAARQPEM
                     IPTLQVKMFIVAGLLDAVTMIGVGIALFMLFANPFIAVVKG"
     gene            complement(1442..2212)
                     /gene="atpB"
                     /locus_tag="PLL14_07865"
     CDS             complement(1442..2212)
                     /gene="atpB"
                     /locus_tag="PLL14_07865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOG03883.1"
                     /translation="MASGEALTSSGYIVHHLTNLTVGHGFWTFHLDSLLVSGLLGLLA
                     FVGMAKLARNATAGVPGGLQTFVEMLVSFIDQQVKDTYHGKSPLVTPMAITIFVWVLL
                     MNTMDLIPVDFVPLVLGWGGVHYFKSVPTTDPNLTFGMSLTVFAIMLIMGIKVKGVGG
                     FLHEVFTVPFGAKMAPVNLLFRIIEDIAKPISLSLRLFGNMYAGEMVFILIALLGLWQ
                     LPLALPWALFHILIITLQAFVFMMLTIVYLSMASESHG"
     gene            complement(2230..2592)
                     /locus_tag="PLL14_07870"
     CDS             complement(2230..2592)
                     /locus_tag="PLL14_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOG03884.1"
                     /translation="MHPQVRWVLGCQLLVTLGAGLVAGVVLGFNAAVSAMIGGGIAMA
                     SALAYVWRAMRPSGRAVADAKMAFNAQVAGEGYKFAVTLLLFALVFKTYAQLAALPLF
                     LTYVATIVVYWMALLRQQ"
BASE COUNT          652 a          868 c          780 g          512 t
ORIGIN      
        1 cgctgatttc ggaaggattc aactgcatgg aaattctcct agttcttgag cgccaaccgc
       61 aggctctcga gttttccgcg tacggaggta tcgagaacct ggtcgccaac cacaatcttg
      121 acgccgccga tcagcgattg atcgacgacc acttcggctg ttagccgggt cttgaagcgc
      181 gcttccaggt cttcgatcag agccttgcgc tgctcgtcgt caaccggaaa ggcactgtag
      241 atcgtggctt ccctgacgcc ttcctgggcg gctctcagcg cctcgaagag accagcgatt
      301 tccggcagag cggacagccg ctcattggta gcgaggacgt cgatcagatt ggcttcgccg
      361 ccttcctgcg tcttgccgac cagcgacttg aagagttcgc tcttctgcgt ggctgagagt
      421 tcggggttgc cgatgcagga acgcatctgc tcatccgtgg cgatcgcaga aagcagcgac
      481 aaccgctcgg accaggccgc cagcgccttg ttttccaacg cgatgcggaa caccgcttca
      541 gcgtaggggc gcgcgatggt aacgagttcg gccatggctt cagagttcct gcttgagcgc
      601 gaccagcatt tcggcatgcg ccttggcatc gacctcgcgc ttcaggatgc gttcggcacc
      661 agccacggcc agatcagcga ctcgcccacg cagctcggcc cgtgcctgct ccacctgctg
      721 ggcaatctcg gccttcgccg aagcgaccac cttgtcggct tcgaccttgg ccacctgctt
      781 cgcctcttca accatctggg caccgcgctt ctctgctgca gagatgatgt cggcagcttt
      841 cgccttggcg tcacgaaggg catcggcgga gcgcttgccg gcaagttcca gctcatgctt
      901 tccgcgctcg ccggcggcca acccgtcggc aatcaatttt tggcgatcag ccatggcctt
      961 cgccagcggc ggccagacgt acttcatggt gatccagatg aagacgccaa accagatcgc
     1021 ttcgccaatc aacgttgcgt tgatgttcac gctaacctcc tgttaagagt tagtccgaaa
     1081 ggagaagcga tcaacccttg acgacggcaa tgaagggatt cgcaaacagc atgaacaggg
     1141 cgataccgac accgatcatg gtgaccgcgt cgagaagacc agcaacgatg aacatcttga
     1201 cctggagagt cggaatcatt tccggttggc gagccgcgcc ttcgaggaag cgcccaccca
     1261 gcagaccaaa tccgatagcg gtaccgagag cacccgcacc gatcaggatg gcgacggcga
     1321 tggcggtatt accaagcaac attgacagag cggcttccat tagtttctcc tagtaagaca
     1381 tgctaaatgc gttaaacaag aacaacgtga atcaaactcg aaagcaggac gactggccac
     1441 gctaaccgtg cgactcgctg gccatcgaca ggtacacgat ggtcagcatc atgaacacga
     1501 aggcctgcag ggtgatgatc agaatatgga aaagcgccca gggcagggcc agcggcaact
     1561 gccagagacc gagcagggca atcaggatga acaccatttc accggcgtac atgttgccga
     1621 acagacgcag cgagagcgag atcggtttgg cgatatcctc aatgatccgg aaaagaagat
     1681 tcaccggcgc catttttgcg ccgaagggga ccgtgaacac ctcgtgcagg aagccgccaa
     1741 cccccttgac cttgatgccc atgatcagca tgatcgcgaa aacggtcagc gacatgccga
     1801 aagtcaggtt cggatcggta gtcggcactg atttgaagta gtgcacgcca ccccaaccga
     1861 ggaccaacgg gacgaagtca accgggatca ggtccatggt gttcatcaac aggacccaga
     1921 cgaaaatggt gatcgccatc ggcgtgacca gcggactctt gccgtggtag gtgtccttga
     1981 cctgctggtc gatgaacgaa accagcattt caacgaaagt ctgcagcccg cccggcaccc
     2041 ccgcggtagc gttcctggcc agcttggcca tgccgacgaa agcaagcagg ccaagcaagc
     2101 cggaaaccag cagggagtcc aggtgaaacg tccagaaacc gtgaccgacc gtgaggttgg
     2161 tcaggtggtg aacaatgtat ccactcgacg ttaacgcctc gcccgaagcc atatcttccc
     2221 cctgtttgac tattgctgcc tgagcagcgc catccaataa acgacaatcg ttgcaacgta
     2281 ggtcaggaag agcggcaagg cggcgagttg cgcataggtc ttgaacacca aggcaaacag
     2341 caacagggtc acggcgaact tgtacccttc accagccacc tgggcattga acgccatctt
     2401 cgcatcggct accgctcgcc cggatggccg catggcccgc catacgtagg ccagagcact
     2461 ggccatggcg atgccaccac cgatcatagc ggacaccgcc gcgttgaacc ccaagaccac
     2521 ccccgccacc agccctgctc ccagtgtgac cagcaattgg cagccaagga cccagcggac
     2581 ctgaggatgc acagctagcc cctccaaaac gttggcagaa taccagactc gctgagggga
     2641 atcaaaccgc aagtgcaaaa aactttgaat tcctcgcaaa catctgcctt gcagccggca
     2701 tccgggccct ttggcaacgc ccccatggtg cgctgcgtca atgacgcaga cgctgcagaa
     2761 taccctctag ctgatcaagg tttccgaagt cgatcaacac cttgccagcg cc
//
LOCUS       DAOSIT010000303         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31157_length_2807_cov_6.515262, whole genome shotgun sequence.
ACCESSION   DAOSIT010000303 DAOSIT010000000
VERSION     DAOSIT010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31157_length_2807_cov_6.515262"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..>2807
                     /locus_tag="PLL14_07875"
     CDS             210..>2807
                     /locus_tag="PLL14_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOG03885.1"
                     /translation="MFTSEVSHEGGGGVKGFRAGANGVDRVPVLEVAEALGLPIKRSG
                     GASYIGCPASAHEHDAERPRCELGGDKPALFFCHKCGAAGDAVGLVKLVQDCDAATAF
                     DWLRAHRFLPQDGNHRRATPADPLERWAAARDFEIATLRAFDITGERGEVFIPERDES
                     GAVIGRVRRRADGTRYSSGDKSLSVKGSKRGLTYSPADLEAGAAAGETLAITEGGPDT
                     LRFRDCGHRAVIGVPSVRPGSRVCAGVAHLAARYGFKRAVIFADPGGLIMRDAVGKAL
                     AAAGLAVAWVVPDPDVDTDGRLRHLPRAERPAELARLIEAALPWTSPAGEADDAADAP
                     AGAPDATGPRPLTDLGNAERFAAQHKGRLLFDVRCNAWRVWDGKRWRLDTTGEAMRRA
                     AQTVRNIYREAAAAADSKQREALANWAKSSESRMRLTALVALAQGLLVIEPDQWDADP
                     WALNVNNGMVDLRTGRLRPHNPADHCTRLAPVDFIEGATHPVLDKVLKEAIPDPEERD
                     FTQRIFGYSLSGEKYEKAVAFVYGPKDTAKSTLVEPVMKALGEYAVTVEPEILMKRDF
                     SNGGGPSPEIARLDGARLVGTSETAEGQKLNSGLVKRISGGGARLTARECHANTREFD
                     VTFLLLFQSNYRPRVAADDDAFFTRLKVIPFDVVIPRDRRDPRIKQTLESDPAAQSAI
                     LWWLVEGCLAWQREGLREPAAVTAATGKYQDEMDPLSGFFDDCCRFSPDARATAKELR
                     EAYENWAKGEGLKERYLVSSKVFGSRLTARGCISGKVNRERGWIGIELSDGTDGTDGT
                     DSPGLFPYIRDCLEETTAKRPKRPMCPTGGTELDRELDEVMAGAQWFADWSAPERATW
                     ARSVLAASNG"
BASE COUNT          505 a          859 c         1002 g          441 t
ORIGIN      
        1 ggtggaacgc ggcgactttg accgatggct gcgggatgaa ttgcagcggc gcccgagccg
       61 tcacgagatt atccgcaagg cgctggccgg cggccggcga ggcgggccga tccactagca
      121 ctgcgcaacc cgcggtcccc gccggcgcac ccgcgcgcgg ctgaaaaaaa acccgccgtt
      181 gcgagcggcg ggacaggcag gaatacgaaa tgtttacatc cgaagtatcg cacgaaggcg
      241 gcggcggcgt caaggggttt cgcgcgggcg cgaacggcgt ggaccgcgtg cccgtgctcg
      301 aagtagcgga agcgctgggc ctgccgatca agcgcagcgg cggcgcgtcc tacatcggct
      361 gcccggcgtc ggcgcatgag catgacgccg agcggccgcg gtgcgaactc ggcggcgaca
      421 agccggcgct gtttttctgc cacaaatgcg gcgcggcggg tgatgcggtc gggctggtga
      481 agctggtcca agactgcgac gcggccacgg cgttcgactg gctgcgcgcg catcggtttt
      541 tgccgcagga cgggaatcat cgccgggcga cccctgctga tccgctcgag cgctgggccg
      601 cggcgcgtga ctttgagatc gccacactga gggccttcga catcacgggc gagcgcggcg
      661 aggttttcat ccccgagcgc gacgaaagcg gcgcggtcat cggccgcgtg cgccggcggg
      721 cggacgggac gcgatacagc agcggcgaca agtcgctgag cgtgaagggc tcaaagcgag
      781 ggctcaccta ctcgccggct gatctcgagg ccggggccgc ggccggcgaa acgctggcca
      841 tcactgaggg cgggccggac accctgcgct tccgtgactg cggacaccgg gccgtgatcg
      901 gcgtgccgag cgttcggccc ggctcgagag tctgcgcggg ggtcgcgcac ctcgccgcgc
      961 ggtatggttt caaacgcgcg gtgatctttg ctgatcctgg cggcctgatc atgcgtgacg
     1021 ccgtggggaa ggctctcgcc gcggccgggc tggcggtcgc gtgggtcgtg cccgatcccg
     1081 acgtcgatac cgatggccgc ctgcgccacc tgccgcgagc cgaacggccg gccgaactgg
     1141 cgcgcctgat cgaagcggcg cttccgtgga cctcgccggc gggcgaggcc gatgacgccg
     1201 cggatgcgcc ggcgggggct cccgatgcga cagggccgcg gccgctaact gatcttggca
     1261 atgccgagcg gtttgcagcg cagcacaagg gccggctgtt attcgatgtc cgatgcaatg
     1321 cctggcgcgt ctgggacgga aaacgctggc gcctggatac gaccggcgag gcgatgcgcc
     1381 gggccgctca gacggtgcga aatatctacc gagaagcggc ggcggcggcc gactcaaagc
     1441 aacgcgaggc gctggcgaac tgggccaagt catccgaaag ccggatgcgt ttgaccgccc
     1501 tggtggcgct ggcgcaaggg cttctcgtta tcgaaccgga ccaatgggac gccgaccctt
     1561 gggcgctgaa cgtgaacaac gggatggtgg accttcggac tggccggctt cgcccgcaca
     1621 acccggcgga ccattgcacc cgactggcgc ccgtcgattt catcgaaggc gcaacgcatc
     1681 cggtgctcga caaggtattg aaggaagcga ttcccgaccc cgaggaacgc gacttcacgc
     1741 aacgcatctt cggctattcg ttaagtggtg agaaatacga gaaggccgtc gcctttgtct
     1801 acgggccgaa ggatacggcc aagtcaactc tggtcgaacc ggtcatgaag gcactgggcg
     1861 agtatgccgt gacagtcgaa ccggagattc tgatgaaacg tgacttcagc aacggcggcg
     1921 gaccctcgcc agagattgcg cgcctggacg gggcgcggct ggtgggaaca tcggaaacgg
     1981 ccgaggggca gaaactcaat tcgggactcg tgaagcgcat cagcggcggc ggcgcgcggc
     2041 tcactgcccg agaatgccat gcaaataccc gcgagtttga cgtgaccttc ctgctgctgt
     2101 tccagtcgaa ctacaggccg cgggtggccg ccgacgatga cgcctttttc acccgcctga
     2161 aggtcatccc gttcgatgtc gtgatccctc gcgaccgtcg cgacccgcgc atcaagcaga
     2221 ccctcgaatc cgaccccgcg gctcagtcgg caatcctctg gtggctggtg gaaggctgcc
     2281 tcgcctggca acgcgagggg ctgcgcgaac cggccgcggt gacggccgcg acgggcaagt
     2341 atcaggatga aatggacccc ttgagcggtt tctttgatga ctgctgccgt ttttcgccgg
     2401 acgcacgagc gacggccaag gaactacggg aagcctatga aaattgggcc aagggcgagg
     2461 ggctaaaaga gcgctatctc gtgtcgtcaa aggtgtttgg ctcgcggctg accgcccgcg
     2521 gatgcatcag cgggaaggtc aatcgggagc gcggttggat cgggattgaa ttgtccgatg
     2581 ggacggatgg gacggatggg acggattcac cgggtttatt tccctatata agagattgct
     2641 tggaagaaac cactgcaaag cgtcccaagc gtcccatgtg tcccacgggg ggcactgaac
     2701 tggaccgcga acttgacgaa gtaatggccg gcgctcaatg gttcgcggac tggtcggcgc
     2761 ccgagcgggc cacctgggca aggtcggtgt tggccgcaag caacggc
//
LOCUS       DAOSIT010000304         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31377_length_2791_cov_10.685673, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000304 DAOSIT010000000
VERSION     DAOSIT010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31377_length_2791_cov_10.685673"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PLL14_07880"
     CDS             complement(<1..1178)
                     /locus_tag="PLL14_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HOG03886.1"
                     /translation="MTTTATTGHPFPRHLMLAALLAAGLGGCATDSQYEPRSPVVSPP
                     PVRATPPPTTPPAALPRPSPVPPASPHERDGRALLNRLLPANIGDRAGWTTDILAALT
                     ALQIPALPENLCAAIAVIEQESSFVADPPVAGLSRIAWQEIERRREKYSIPRLVLDLA
                     LRKSSPDGRTYRARIDALQTERQMNALYDDMLSELPGGKTLFGGYNPIRTGGPMQVSI
                     AFAEEQIRQRPYPYVRRDSVRNEVFTRRGGVYFGMAMLLDYPGSYSQMLYRFADFNAG
                     RYSSRNAAFQDAVSRLSGQTLALDGDLLRYQDGVPADGSSETQRAIFLLRARLRLNEA
                     EIVRDLKLEKSPAFEHSVLYQRLYALADATSGAPRPREHLPQIDLKSPKITRQLTTEW
                     "
     gene            complement(1181..>2791)
                     /locus_tag="PLL14_07885"
     CDS             complement(1181..>2791)
                     /locus_tag="PLL14_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOG03887.1"
                     /translation="ASAAGLAMLQAGGAAVDAAVAAQMVLTLVEPQSSGIGGGALLLH
                     YDGRDTVAFDGRETAPAAVSETLFSKPDGQPMRFHEAVVGGRAVGVPGTVRLLEMAHA
                     EYGKLPWASLFEPAIRLAEDGFAVGQRLHTLLQADPYLRKDPQAAAYFYGRDGQAAAI
                     GSTLRNPELAEVLRRIARDGSRALHEGEIAQAVVDKVQGHPENPGQLSLGDMATYQPR
                     RRTPFCHRHTPRAVVPPRSYVICGFPPPASGAIAIAQILGILDHTEAPALAFSDPLWM
                     HYYGEASRLAFADRAQYVADPDFVAAPGGDWQALISPAYLAERARLIGPRSMRLASPG
                     TPSAQATRQAPMPEQAEHGTSHLSVVDGYGNALALTTTIEDAFGARQMVRGFLLNNEL
                     SDFSFTARDERGTPIANRVEPGKRPRSSMSPTLVFDAQTGELVMSGGSPGGALIIHFT
                     AKLLYATSYWGLGPQQAIDLPNFGSLNGPILLEEKRFTASFIAALQARGHEVREIPMN
                     SGLQAILKNAGGYAGGADPRREGRVMGE"
BASE COUNT          424 a          886 c         1005 g          476 t
ORIGIN      
        1 aaccactcgg tggtcagttg gcgggtgatc tttggacttt tcaggtcgat ctgcggcagg
       61 tgttcgcgtg gccgcggcgc gccgctggtc gcatcggcca gggcgtagag gcgctggtag
      121 agcacgctgt gttcgaaggc cggagacttt tcgagtttga gatcgcgcac gatctcggct
      181 tcattgaggc gcaggcgtgc gcgcagcagg aagatcgccc gttgggtctc gctgctgccg
      241 tctgccggga cgccgtcctg gtaacgcagc aggtcgccgt cgagcgccaa cgtctgaccg
      301 ctgaggcggc tgacggcgtc ctggaacgcc gcgttgcggc tgctgtagcg gccggcgttg
      361 aagtcggcaa agcggtagag catctggctg taggagcctg ggtagtcgag cagcatggcc
      421 attccgaaat agacgccgcc gcgacgtgtg aacacctcgt tgcgcacgct gtcgcggcgc
      481 acgtaggggt aagggcgctg gcgaatctgt tcctcggcaa aggcgatgct cacctgcatc
      541 gggccgccgg tacggatcgg gttgtagccg ccgaaaagag tcttgccgcc cggcaactcc
      601 gagagcatgt cgtcgtagag cgcgttcatc tgtctttcgg tctgcaaggc gtcgatgcgc
      661 gcccggtagg tgcgtccgtc cggggaggac ttgcgcaggg caaggtccag gaccagcctg
      721 gggatgctgt acttttcccg tcgccgctcg atttcctgcc aggcgatgcg cgacagcccg
      781 gcaaccggcg ggtccgcgac gaagctcgac tcctgttcga tgaccgcgat cgcggcacag
      841 agattttccg gcagcgccgg gatctgcagg gcggtgagcg ccgccagaat gtccgtcgtc
      901 cagccggcac ggtcgccgat gtttgccggc agcagacggt tgagcaaggc gcggccatcg
      961 cgttcgtgtg gggaggctgg cgggacgggc gacggacgcg gtagcgctgc cggaggcgtc
     1021 gtaggcggtg gggttgcgcg caccggcggc ggtgagacca ccggcgatcg gggttcgtat
     1081 tggctgtcgg tggcgcagcc gccgaggccg gcggcaagca gagcggcgag catcaggtgt
     1141 cgcgggaagg gatgcccggt tgtggctgtg gtggtcactt tcactcgccc atcacccgtc
     1201 cctcacgccg cggatcggcg ccaccggcgt aaccgccggc atttttcaga atggcttgca
     1261 ggccgctgtt catcgggatt tccctgactt catggccacg ggcctggagt gccgcgatga
     1321 atgacgcggt gaagcgcttt tcctcgagca gtatcgggcc gttcaacgag ccgaaattgg
     1381 gcaggtcgat cgcctgctgc ggccccaggc cccagtacga agtcgcgtag agcagcttgg
     1441 ccgtaaagtg gatgatcagc gcgccgcctg gcgagccgcc gctcatcacc aactcgccgg
     1501 tctgcgcatc gaagaccagt gtcggactca tcgacgagcg tgggcgtttg ccgggctcga
     1561 cgcggttggc gatcggcgtg ccacgctcat cgcgggcggt gaaggaaaaa tcgctgagtt
     1621 cgttgttgag cagaaaacct ctgaccatct ggcgggcacc gaaggcgtcc tcgatggtcg
     1681 tggtcagcgc cagcgcgttg ccgtagccgt cgacgacact gaggtgcgag gtgccgtgct
     1741 cagcctgctc cggcatcggt gcctggcgcg tggcctgtgc cgatggcgtc cccggactgg
     1801 ccagccgcat gctccgcggg ccaatcagcc gcgcacgctc ggccaggtag gcggggctga
     1861 tcagcgcctg ccagtcgccg cccggcgcgg cgacgaaatc cggatcggcg acatactggg
     1921 cacggtcggc gaaagccagg cgtgacgctt cgccgtaata gtgcatccac agcgggtctg
     1981 aaaacgccag tgcaggcgcc tcggtgtggt cgaggatacc gaggatctgc gcaatggcga
     2041 tggccccgga agcggggggc ggaaaaccgc agattacgta acttcgcggc ggcaccaccg
     2101 ccctgggcgt gtgccggtgg cagaacggcg tacgccgcct cggctggtag gtggccatgt
     2161 cgccgaggct cagttggccg ggattttcgg gatgcccctg caccttgtcg accaccgcct
     2221 gcgcgatctc gccctcgtgc agcgcccgcg atccgtcgcg cgcgatccgg cgcaggacct
     2281 cggcaagttc ggggttgcgc agcgtgctgc cgatggccgc cgcttgtccg tcccgaccgt
     2341 agaaatacgc ggcggcctgc gggtccttgc gcaggtatgg atcggcctgg agcaaagtgt
     2401 gcaggcgctg gccgaccgcg aagccgtcct cggccagccg gatcgctggc tcgaagaggc
     2461 tcgcccaggg cagcttgccg tactcggcgt gcgccatttc gagcaggcgt acggtacccg
     2521 gcacgccgac cgcgcgccca ccgacgacgg cttcgtgaaa acgcatcggc tggccgtcgg
     2581 gcttgctgaa cagcgtttca ctgactgccg ccggcgccgt ctcgcggccg tcgaaggcca
     2641 ccgtatcacg cccgtcgtag tgcagcagca aggcgccgcc gccgatgccg ctggactggg
     2701 gttcgaccag ggtcaacacc atctgcgcgg cgaccgccgc gtccaccgcc gcgccgccgg
     2761 cctggagcat tgccagtccg gcggcgctgg c
//
LOCUS       DAOSIT010000305         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31463_length_2785_cov_8.972527, whole genome shotgun sequence.
ACCESSION   DAOSIT010000305 DAOSIT010000000
VERSION     DAOSIT010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31463_length_2785_cov_8.972527"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..2724
                     /locus_tag="PLL14_07890"
     CDS             136..2724
                     /locus_tag="PLL14_07890"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOG03888.1"
                     /translation="MPAHNALIAHFKAHLQEGQRAIREHFAASGDAARLLDERCRLVD
                     QVLCELWRELDLPASLALVAVGGYGRGELYPASDVDLLILLPEQPGSALTARLERLIC
                     VFWDIGLETGHSVRTIEQCLEEASADITVQTAMIEARLLTGDALLFQGFTAVLKASLD
                     APAFFQTKRMEQEDRHQRFSDTPYSLEANCKEGPGGLRDLQLILWIAQAAGYGRCWRD
                     LEQRGFITAEEQQLLETCEAFLCRLRTHLHLHVGRREDRLLFEYQTALAERLGFAATA
                     TRRSSEQLMQRYYRTAKAVTQINAIVLQNIGAALFPPPEGPPQPINERFQNTNEFLDV
                     VHEDVFNHNPGALLEAFLLLQQHAELQGMSARTQRALWRARHLIDDDFRRQPENRQHF
                     LQLFKQGRRLVQEFRRMNQLGVLGRYLPGFGRIVGQMQHDLFHVYTVDQHILQVVRRL
                     RRFSMEEFAHEYPFCSRLLSDLGNPWVLYIAALFHDIAKGRGGDHSELGAVDAQEFCE
                     DHGLAAADTELVVWLVRRHLLMSSVAQKQDISDPGVVARFAAIVGDERHLIALYLLTV
                     ADIRGTSPKVWNAWKGQLLEQLFNATRRFLLSNDSTLMTQGIIAQRQQEAMRLMRFFA
                     LSEKAHEGLWQQLDTVYFLRQSAEEIAWHACALHERIEIDQPLVRARINSLGAGLEVM
                     VYTHDQPDLFVRMVGFFSRAGYSIVDAKIHTTRHGYALDTFMLLDVSGRDSDRAMISY
                     IEHELGERLLRQAPADVPANGRISRQVKHFPIQPQVSIQPLVSLEADETGKLFVLSVV
                     AADRPGLLFVIARKLADHGANLHTAKIATLGERVEDTFLISGGHLEESAGRVKLETDL
                     LKHLLV"
BASE COUNT          523 a          928 c          829 g          505 t
ORIGIN      
        1 gccgatcgat gcttcgttgc acacctggaa actccacctc gaaggtagtc ggcaccttgc
       61 cgggcctgcc ccggcgttgc ggctgcggcg tacaggtggc tacgtgcccc cgggccggga
      121 acggtgaaac cggaagtgcc tgcccacaac gccctgatcg cgcacttcaa ggcccacctg
      181 caggaaggac agcgcgccat acgcgagcat ttcgcagcca gcggcgatgc agcgcgactc
      241 ctcgacgaac gctgccgcct tgtcgaccag gtgttgtgcg agctctggcg cgagcttgat
      301 ctaccggcct cgctggcgct ggtcgccgtt ggcggctacg gccgcggcga gttgtacccg
      361 gcttccgatg tcgatctgct gatcctgctt cccgaacaac ccggcagcgc cctgactgcc
      421 cgtctcgagc gactgatttg cgtgttctgg gacatcggcc tcgaaaccgg ccatagtgta
      481 cggaccatcg aacaatgcct ggaagaagcc agtgccgaca tcaccgtgca gacggcgatg
      541 atcgaggccc gcctgctcac cggcgatgcg ttgctttttc agggatttac cgcggtcctc
      601 aaggccagtc tcgacgcgcc cgccttcttc cagaccaagc gcatggagca ggaggaccgc
      661 caccagcgct tcagcgacac cccttacagc ctcgaagcga actgcaagga aggacccggc
      721 ggactgcgcg acctgcaact gatcctctgg attgcccagg cggcaggcta cggcaggtgc
      781 tggcgggatc tcgaacagcg cggtttcatc accgccgagg agcaacaact gctcgaaacc
      841 tgcgaggcct tcctgtgccg cctgcgcacc cacttgcacc tgcacgtcgg gcgccgtgag
      901 gatcgcctgc tgttcgaata tcagacggcg ctggccgaac gactgggatt cgccgccacc
      961 gccacgcgcc gctccagcga gcagttgatg cagcgctact accggacggc gaaggcggtc
     1021 acccagatca atgcgatcgt tttgcagaac atcggcgcgg cgcttttccc accgccggaa
     1081 ggcccaccgc aaccgatcaa cgaacgcttc cagaatacca acgagttcct cgacgtggtg
     1141 cacgaggatg ttttcaacca caaccccggc gccctcctcg aagcctttct gctgctacag
     1201 caacatgccg aactgcaggg catgagtgcg cgtacgcagc gtgcgctgtg gcgcgcacgt
     1261 catctgatcg acgacgattt ccgtcgccag cccgagaacc ggcagcattt cctgcagctc
     1321 ttcaagcaag ggcgtcgcct ggtccaggag ttccggcgca tgaaccagct tggcgtcctg
     1381 ggtcgctacc tgccgggttt cggacgtatc gtcggtcaga tgcaacacga tctgttccac
     1441 gtatacactg tcgaccagca catcctgcag gtggtacgcc ggctgcgccg tttctcgatg
     1501 gaagagttcg ctcacgaata cccgttctgc agtcgcctgc tcagcgacct gggaaacccc
     1561 tgggtactct atattgccgc cctgtttcac gatatcgcca aggggcgggg tggtgatcat
     1621 tccgaactcg gggcggtcga tgcgcaggag ttctgcgagg accacggcct ggcggcggca
     1681 gacaccgaac tggtcgtctg gctggttcgc cgccacctgc tgatgtccag cgtcgcccag
     1741 aagcaggata tctccgaccc tggcgtggtc gcccgctttg ccgcgatcgt cggcgacgag
     1801 cggcatttga tcgcgctcta cctgctgacc gttgccgaca tccgcggtac cagcccgaag
     1861 gtctggaacg cctggaaggg acaactgctg gagcaactgt tcaacgccac ccggcgcttc
     1921 ctcctgtcca atgatagtac cctgatgacc cagggcatca ttgcccagcg tcaacaggaa
     1981 gcaatgcgcc tgatgcgctt tttcgcgctg tccgagaagg cgcacgaagg cctctggcaa
     2041 caactcgaca cagtgtattt cctgcgccag tcggccgaag aaattgcctg gcacgcgtgc
     2101 gccctgcacg agcggatcga gatcgatcag ccgctggtgc gggcacgcat caattcgctc
     2161 ggcgccgggc tcgaagtgat ggtctacacg cacgaccagc ctgacctgtt cgtgcgcatg
     2221 gtcggcttct tcagccgtgc cggctacagc atcgtcgacg ccaaaatcca cactacgcgc
     2281 catgggtatg cgctcgacac cttcatgctg ctcgacgtca gtggccgtga cagcgaccgt
     2341 gcgatgatca gctacatcga acacgagctc ggcgagcgcc tgctgcggca ggcaccggca
     2401 gacgtacccg ccaacgggcg catctcgcgg caggtcaaac atttcccgat ccagccacag
     2461 gtgagcattc aacccctggt cagcctggaa gcggacgaga ccggcaagct cttcgtgctg
     2521 agcgtcgttg ccgccgatcg acccggcctg ctgttcgtca tcgccagaaa gctcgctgac
     2581 catggagcca acctgcacac cgccaagatc gccaccctcg gtgagcgggt cgaagacacc
     2641 ttcctgatca gcggcgggca tctcgaggaa agcgccgggc gggtcaagct ggaaacggat
     2701 ttgctgaagc atttgctggt ctgagtcagc gacgctgcag cacgccccat tgaagtgcca
     2761 caatgcggtt ttcgtgcctt cacga
//
LOCUS       DAOSIT010000306         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_31770_length_2764_cov_7.715024, whole genome shotgun sequence.
ACCESSION   DAOSIT010000306 DAOSIT010000000
VERSION     DAOSIT010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31770_length_2764_cov_7.715024"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLL14_07895"
     CDS             complement(<1..168)
                     /locus_tag="PLL14_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOG03889.1"
                     /translation="MPPENKLPDAVQRVTDLLARNGHAVTIVMLPATGKTSAQAAAGL
                     GCNVAEIAKSIV"
     gene            430..>1553
                     /locus_tag="PLL14_07900"
     CDS             430..>1553
                     /locus_tag="PLL14_07900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOG03890.1"
                     /translation="MLDRPDEHQEIRDAVRALCRQFPDAYFRAIDAQRAYPEAFVDAL
                     IGAGWLSAMIPEAYGGSGLGLTAASVIMEEINRCGGNSGAVHGQMYNMGTLLRHGSRA
                     QKEKYLPAIAAGKLRLQSMAVTEPSTGTDTTRIRTTAVRKDGRYLVNGQKVWISRVQH
                     SDLMILLARTTPLAEAPRKSEGLSIFVVDLHDAVGNGLSVQPIANMVNHETNELFFDN
                     LEVPAENLIGEEGQGFRYLLDGLNAERALIAAECIGDGYWFIERASRYASERIVFDRP
                     IGMNQGVQFPIAEAFIEIEAANLMRFKACALFDAQQPCGAQANMAKYLAAKASWEAAN
                     VCLQTHGGFGFANEYDIERKFRETRLYQVAPISTNLIFSYV"
     assembly_gap    1554..1653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1654..2538
                     /locus_tag="PLL14_07905"
     CDS             <1654..2538
                     /locus_tag="PLL14_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOG03891.1"
                     /translation="AAARLGLSYAALNAKHPRLIVCDISGYGSDGPYRDRKAYDLLIQ
                     SESGFLSVTGESDTPAKAGCSIADIAAGMYAYSHILAALLQRGRSGCGCHIEVSMLES
                     MVEWMSYPLYYAFEGAAPPPRAGAAHATIYPYGPFPAGDGKMVMLGLQNDREWHVFCL
                     QVLERPDLAGDPRYASNALRTAAREELQPLIVECFACLNSEQLIARLDAAQIANARMN
                     DMHDVWQHPQLAARQRWIQVQTPAGPIPALLPPGEIDAFTPRMDAVPALGEHSEAILG
                     ELGWEPPEIARLRAIGAI"
BASE COUNT          480 a          828 c          855 g          501 t
ORIGIN      
        1 gacgatggat ttggcgattt cggcgacgtt gcacccgaga ccggctgcgg cctgggcaga
       61 agtcttgccg gttgccggca gcatgactat cgttacggcg tggccgttcc tggcgagcag
      121 gtcggtaact cgctgcaccg catcggggag tttgttttca ggcggcatgg gtaccttcca
      181 tggctctggc ggtggcgtgc gccgccgggg ggtggcattc gggctgtgcg tgggtggcgg
      241 caggagtatc gcacacgaag ccggcgacag cacacggtac tgccatcagg aaagttcctt
      301 caggcgcgtc gaaggtccgt tgttggcgac tgcgtcagcg ctgcccagcg gcgataatga
      361 cgggtgcgct ggccgccgac cgaagcctgt ggtccctggc gcaagtcaac gccagttcag
      421 gagacagcca tgctcgaccg acccgacgaa caccaggaaa tccgcgatgc cgtgcgtgcc
      481 ctttgccgtc agtttccgga cgcgtatttc cgggcgatcg acgcgcagcg cgcttatccc
      541 gaggctttcg tcgatgcgct gatcggtgcg ggctggttgt cggcgatgat tcccgaggct
      601 tatggcggtt cgggcctggg attgaccgcg gcgagcgtga tcatggaaga gatcaaccgt
      661 tgcggcggca attccggcgc cgtgcatggc cagatgtaca atatgggcac gctcctgcgc
      721 cacggcagcc gcgcccagaa ggaaaaatat ctgccggcga tcgccgccgg caaactgcgc
      781 ctgcagtcga tggcggtcac cgaaccgagt accggaaccg acaccacccg catccggacc
      841 accgcggtca ggaaggacgg ccgttacctg gtcaatggcc agaaggtctg gatttcgcgg
      901 gtgcagcact cggacctgat gatcctgttg gcgcgtacga cgccgcttgc cgaagcgccc
      961 cggaaatccg aaggcctgtc gatctttgtc gtcgatctgc atgacgcggt cggcaatggc
     1021 ctctcggtgc aaccgatcgc gaacatggtc aatcacgaga ccaacgaact gttcttcgac
     1081 aacctcgaag ttcccgccga gaacctgatc ggcgaggaag gccagggctt ccgctatctt
     1141 ctcgacggcc tcaacgccga gcgcgcgctg attgccgccg agtgcatcgg cgacggctac
     1201 tggttcatcg aacgcgccag ccgctacgcc agcgagcgca tcgttttcga ccggccgatc
     1261 ggcatgaacc agggggtcca gttcccgatc gccgaggcat tcatcgagat cgaggcggcg
     1321 aacctgatgc gtttcaaggc ctgcgccctg ttcgatgcgc agcagccctg cggcgcgcag
     1381 gccaacatgg ccaagtacct tgccgccaaa gcctcctggg aggcggccaa cgtctgcctg
     1441 cagacccacg ggggcttcgg cttcgccaac gaatacgata tcgagcgcaa gtttcgcgaa
     1501 acgcgcctgt atcaagtcgc gccgatctcg accaacctga tcttctccta cgtnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgccg cgcgtcttgg tttgtcgtac
     1681 gcggctctga acgcaaagca tccgcgactg atcgtctgcg acatttccgg ttacggtagc
     1741 gacgggccct atcgcgacag gaaggcctac gatttactga tccagagcga gtcaggcttc
     1801 ctctccgtca ccggcgagtc cgacacgcca gccaaggccg gttgctcaat cgccgacatc
     1861 gcggccggca tgtacgccta cagccacatc ctggccgcgc tgctgcagcg cggcaggagc
     1921 ggttgtggtt gccacatcga ggtgtcgatg cttgaaagca tggtcgagtg gatgagttat
     1981 ccgctgtact atgctttcga aggagcggcg ccgccaccgc gtgccggcgc cgcgcacgcg
     2041 acgatctatc cgtatggtcc gttcccggca ggtgacggca agatggttat gctgggcctg
     2101 caaaacgatc gcgagtggca tgtgttctgc ctccaggtcc tcgaacgtcc cgatcttgcg
     2161 ggcgatccac gctacgcctc gaacgccttg cgcacggccg cccgcgaaga actgcaaccg
     2221 ctgatcgttg aatgtttcgc gtgcctgaat tcggaacaac tgatcgccag gctggacgcc
     2281 gcgcagattg ccaacgcgcg catgaacgac atgcacgacg tctggcaaca cccgcaactg
     2341 gcggcacgtc agcggtggat ccaggtgcag acaccggccg gcccgattcc ggccctgctg
     2401 cctcccggcg agattgatgc cttcacgccg cggatggatg cggtgcccgc tctgggcgaa
     2461 catagcgaag ccatccttgg cgaactgggt tgggaaccgc cggagattgc ccgtttgcgt
     2521 gccatcgggg ccatatgaat gtggccccga acgcggattc ctcccgctcg aaggcttccg
     2581 ttgggtccgg caaatgcgcg gccgacgatc gggaggatgg cactggtttg atgcagggag
     2641 tcaaacagtc gccgtggcga tgctgccctt tgataagatg gctgggggag acctgccggg
     2701 cggtgtgaag ctgttctgca cttttcttcc ggattttggg tacagtacac ttgcaccaga
     2761 ctaa
//
LOCUS       DAOSIT010000307         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32003_length_2748_cov_7.740438, whole genome shotgun sequence.
ACCESSION   DAOSIT010000307 DAOSIT010000000
VERSION     DAOSIT010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32003_length_2748_cov_7.740438"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLL14_07910"
     CDS             <1..843
                     /locus_tag="PLL14_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOG03892.1"
                     /translation="THDFILCFTNRGRVYWLKVYEVPQGTRTSRGKPIVNLFPFEDGE
                     KISAILPVKEFAEDRYIFMCTANGTVKKTPLSDFSNPRKAGIIAVALDEDDVLIGVEI
                     TNGAQDVVLVSDAGKAVWFDEEDVRPMGRTARGVRGMRLAEQQKVLSLLVADNDQQTV
                     LVATENGFGKRTALADFRHSGRGTQGVIAIAASERNGKVVAARLVRDDDEIMLITTGG
                     VLIRTRVREIRELGRATQGVTLIALDAGEKLAGLEKVIETEDEAESPGQSLAETPEAD
                     PGGE"
     gene            923..2014
                     /gene="pheA"
                     /locus_tag="PLL14_07915"
     CDS             923..2014
                     /gene="pheA"
                     /locus_tag="PLL14_07915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOG03893.1"
                     /translation="MNDDLLRELSVVRQQIDALDGQLLELLNQRARYAQQVGEIKARH
                     GEAGFIYRPEREAQVLQRIQGSNRGPLSNESVTWFFREVMSACLSLEQPLGIAFLGPL
                     GTFSEGAATKHFGHAARLMPQISIDDVFREVEACHADYAVVPIENSTEGAIGRTLDLL
                     LTTPLKICGEVLLRIHQHLLSNELALGAVKKVYSHAQSLAQCHEWLNRSLPLAQRIPV
                     GSNAQAAQLAAAEAGAAAIAGQAAAERYHLPELASNIEDEPNNTTRFVVLGRHDAGPS
                     GRDKTSLVMSAHNQTGALGKLLAPLSDAGVSMTRLESRPARHTLWEYVFFVDIDGHSD
                     DAPVRAALVELARRAPYLKLLGSYPMAAY"
     gene            2042..>2748
                     /locus_tag="PLL14_07920"
     CDS             2042..>2748
                     /locus_tag="PLL14_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03894.1"
                     /translation="MAIHEQSLPYIRAISPYQPGKPITQLAREMGLAVEQIVKLASNE
                     NPLGMSPKARVAVHEAIGGLARYPDDFALKQALAERTGLGMEQIVLGNGSNDVLDLVA
                     RVFLAPGRSAIFAQYAFAVYPLATLAAGGEPIAVAARDHGHDLDAMRTAIRPDTRLLW
                     IANPNNPTGTFVPYPQLRTFLQSLPEDVIVVLDEAYNEYLPPAERVDTTAWLATMPNL
                     VITRTFSKIYGLAGLRVG"
BASE COUNT          485 a          900 c          890 g          473 t
ORIGIN      
        1 acccacgatt tcatcctctg cttcaccaac cgtgggcggg tctattggct caaggtctat
       61 gaagtgccgc aggggacgcg taccagtcgc ggcaagccga tcgtcaacct ctttcccttc
      121 gaggacggcg agaagatcag cgccatcctg ccggtcaagg agttcgccga ggatcgctac
      181 atcttcatgt gcaccgccaa cgggacggtg aagaagacgc cgctgtccga tttctccaat
      241 ccgcgcaagg ccggcatcat cgccgtcgcg ctcgacgagg atgacgtcct gatcggcgtc
      301 gagatcacca acggcgcgca ggacgtggtc ctcgtctccg atgccggcaa ggccgtgtgg
      361 ttcgacgaag aggacgtgcg gccgatgggg cgcacggcgc gcggtgtacg cggcatgcgt
      421 ctggccgagc agcagaaggt gttgtcgctg ctggtcgccg acaacgatca gcagaccgtt
      481 ctggtggcca ccgagaacgg tttcggcaag cgcacggcgc tggccgattt ccggcattcc
      541 gggcgcggca cgcaaggggt catcgccatc gccgccagcg agcgcaacgg caaggtggtg
      601 gccgcccggc tggtacgtga cgacgacgaa atcatgctga tcaccaccgg cggggtgctg
      661 atccgcacgc gcgtgcgcga gattcgcgaa ctcggtcgag ccacccaggg ggtgacgctg
      721 atcgccctcg acgccggtga aaagcttgcc ggtctggaaa aagtcatcga gaccgaagac
      781 gaggccgagt cacccgggca gtcgttggcc gagacgcccg aagccgaccc gggcggcgaa
      841 tgatgcctgg tcgatcgatg ctcgccgtgc ggcggaccgc cggtctggcg accgtccggt
      901 cgtgcgctgt gggcaggcgg cgatgaacga cgatctcctg cgcgaactct ctgtcgtcag
      961 acagcagatc gacgcgctcg acgggcagtt gctggagttg ctgaatcagc gggccagata
     1021 tgcgcagcag gtcggagaga tcaaggcgcg ccacggcgag gccggcttca tctaccgccc
     1081 ggagcgtgaa gcgcaggtcc tgcagcgaat ccagggaagc aaccgcggcc ccttgtcaaa
     1141 cgagagtgtg acctggtttt tccgggaagt gatgtcggcc tgcctgtcgc tcgaacagcc
     1201 gctgggcatc gccttcctgg ggccgctcgg caccttctcg gaaggcgccg caaccaagca
     1261 cttcggacac gcggcacgct tgatgccgca gatctcgatc gacgatgtct tccgcgaagt
     1321 cgaggcctgt catgccgact acgcggtggt cccgatcgaa aactcgaccg aaggcgccat
     1381 cggacgtacc ctcgatctgc tgctgacgac gccgctcaag atctgcggcg aggtgctgct
     1441 gcgcatccat cagcacctgc tgtcgaacga gcttgccctt ggcgcggtga agaaggttta
     1501 ttcgcacgcg cagtcgctgg cgcaatgcca cgagtggctg aaccgctcgc tgcctttggc
     1561 gcagcgcatt ccggtcggga gcaatgccca ggccgcgcag ctcgccgccg ccgaggcagg
     1621 tgccgcggcg attgccggcc aggcggcggc cgagcgctac catctgcccg aactggcgag
     1681 caacatcgag gatgaaccga acaacactac ccgcttcgtc gtccttggcc gccacgatgc
     1741 cgggccctcg gggcgtgaca agacctcgct ggtgatgtcg gcacacaacc agaccggcgc
     1801 cctcggcaag ttactggcgc cgctgtccga tgccggagtt tcgatgaccc ggctggagtc
     1861 ccggccggca cggcacacgc tttgggaata cgtttttttc gtcgatatcg acggccacag
     1921 cgacgatgcg ccggttcggg cggcgctcgt cgaactcgcc cgccgcgcac cctacctgaa
     1981 gctgctgggc tcctatccga tggccgccta ctgagcggac gggttttttc gaggatcgct
     2041 gatggcaatt cacgagcagt cgctaccgta tatccgggcc atttcgccct atcagccagg
     2101 caaaccgatc acccagctgg cacgcgagat gggtcttgcc gtcgagcaga tcgtcaagct
     2161 ggcgtccaac gagaatccct tgggcatgag tcccaaagca cgcgtggccg ttcatgaggc
     2221 gatcggcggg ctggcgcgtt acccggacga tttcgcgctc aagcaggcgc tggccgaacg
     2281 caccggcctg gggatggaac agatcgttct cggtaacggc tcgaacgacg tgctcgatct
     2341 ggtcgcccgc gtctttctgg cgccgggccg ttcggcgata tttgcgcagt acgcctttgc
     2401 cgtttatccg ctggccacgc tggccgccgg cggcgaaccg attgccgtcg ccgcgcgcga
     2461 tcacggccat gacctcgacg cgatgcggac ggcgatcagg cccgatacac gactgctgtg
     2521 gattgccaac cccaacaacc cgaccggcac cttcgtgccg tatccgcaac tccggacgtt
     2581 tctgcagtcg cttccggaag atgtcatcgt cgtgctcgac gaagcctaca acgagtacct
     2641 gcctcccgcc gagcgcgtgg acaccaccgc ctggctggcg acgatgccga atctggtgat
     2701 tacccgcacc ttctccaaga tctacggcct ggcgggcctg cgcgtcgg
//
LOCUS       DAOSIT010000308         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32028_length_2746_cov_10.979933, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000308 DAOSIT010000000
VERSION     DAOSIT010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32028_length_2746_cov_10.979933"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1562)
                     /locus_tag="PLL14_07925"
     CDS             complement(<1..>1562)
                     /locus_tag="PLL14_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG03895.1"
                     /translation="VTLPPQHALILAGAGSGKTRVLTTRIAWLISTGQVGPQGILAVT
                     FTNKAAREMQSRLAAMLPINTRGMWIGTFHGLCNRFLRAHYREAGLPAQFQILDAADQ
                     LAAIKRLLKNLNVDDQKFPPRELMHFINAHKEQGIRAAQAEAYDQFTSRRVAWYAEYE
                     AQCQREGVVDFAELLLRSYELLQRNEPLCHHYQARFRHILVDEFQDTNRLQYAWLKLL
                     AGRGGVPPEGGGACLFAVGDDDQSIYAFRGAEIGNMRDLQREFELANVIRLEQNYRSH
                     GNILDAANSLIKQNRGRLGKNLWTEAGAGEPIRVFEAFSDIDEARFIVEEIGDLVRAG
                     APRDQMAVLYRSNAQSRVLEHQLFTLAVPYRVHGGLRFFDRQEVKHALAYLRLIANPD
                     DDTAFARVVNFPTRGIGTRSIEALQEAARCANSSLYNAAASLAGKAGTSLARFVRLIE
                     DLRSETRDLLLPEVIDRIIDRSGLRQHYLTDKEGQDRLENLDELINAATGFLREEGAA
                     SGDGDAVPDGGPL"
     assembly_gap    1563..1662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1663..2490
                     /locus_tag="PLL14_07930"
     CDS             <1663..2490
                     /locus_tag="PLL14_07930"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG03896.1"
                     /translation="PPEVIEKTLKGIAIPSRPQVLVHLHNELAKDDPDPRVITRLISG
                     DVGLSAAVLKTVNSPFFGLSRRMSSVEQAVSLLGMRSATQIVTGLLLRTAIAGKAGSL
                     ERFWDSAEKVAGIASHIALSIPHGPRDEAYCFGLFHDIGIAILMQKFPDYRQTLAMAE
                     QIIDRPITAVEEERHATDHATLGYLVAKGWFLPEVICDGILYHHDPSVFDDREVTSSQ
                     ALTLIAINALAEHFHDEYLHLRHNSNWQRMGDRALDQLGLREDEYCDLREDLTKMAG"
     gene            complement(2540..>2746)
                     /locus_tag="PLL14_07935"
     CDS             complement(2540..>2746)
                     /locus_tag="PLL14_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG03897.1"
                     /translation="PVNGFDAAGYRIGYGGGYFDRTLASVSPRPLAVGVGFELARLDS
                     ICPEPHDQRLDVMVTEAGVFRPSS"
BASE COUNT          498 a          821 c          850 g          477 t
ORIGIN      
        1 agcggcccgc catccggcac agcatcgcca tcacccgacg cagccccctc ctcgcggagg
       61 aagcccgtgg ccgcgttgat cagttcgtcc aggttctcga gtcggtcctg gccttccttg
      121 tcggtcagat agtgctggcg cagaccgctc cggtcgatga tccggtcgat cacctcgggc
      181 aggagcaaat ccctggtctc gctgcgcaag tcctcgatca ggcggacgaa gcgggcgagt
      241 gacgtgcccg ccttgccggc gagactcgcc gccgcgttgt acaggctcga attggcgcaa
      301 cgcgccgctt cctgcagggc ttcgatgctg cgcgtaccga tcccgcgcgt cggaaaattc
      361 acgacgcggg caaaggccgt gtcgtcgtcg gggttggcga tcaggcgcag gtaggccagg
      421 gcatgtttga cttcctggcg atcgaagaag cgcaggccgc catgcacacg atacggcacc
      481 gccagggtga acaactggtg ttcgagcacc cgcgattgtg cgttggagcg gtacaggacg
      541 gccatctgat cgcgcggtgc gcctgcgcgc acgagatcgc caatctcctc gacgatgaag
      601 cgcgcttcat cgatatcgga aaaggcctcg aagacgcgga tcggttcgcc ggcgccggct
      661 tcggtccaca gattcttgcc gaggcggcca cgattctgtt tgatcagcga attggcggcg
      721 tcgaggatat tgccatgcga gcgatagttc tgttccaggc gaatcacgtt cgccagttcg
      781 aattcgcgct gcaggtcgcg catgttgccg atctcggcgc cgcggaaggc gtagatcgac
      841 tggtcgtcat cgcccaccgc gaagaggcag gcgccgccgc cttccggagg cacgccgccg
      901 cgcccggcca gcagcttcaa ccaggcgtac tgcaggcggt tggtgtcctg aaactcatcg
      961 accagaatgt gccggaagcg cgcctgatag tggtggcaca gcggctcgtt gcgttgcagc
     1021 agttcgtacg accgcagcaa cagttcggca aagtcgacca ccccttcacg ctggcattgc
     1081 gcttcatact cggcgtacca cgcgacgcgg cgactggtga actggtcgta ggcttcggcc
     1141 tgtgcggcgc gaatcccctg ctccttgtgg gcattgatga agtgcatcag ttcgcgcggc
     1201 ggaaacttct ggtcgtcgac gttgaggttc ttgagcaggc gcttgatcgc cgccaactgg
     1261 tccgcggcat cgagaatctg gaactgcgcg ggcaacccgg cctcgcggta gtgcgcgcgc
     1321 aggaagcggt tgcagaggcc gtggaaggta ccgatccaca tgccgcgggt gttgatcggc
     1381 agcatcgccg ccaggcggga ctgcatctcc ctggccgcct tgttggtaaa ggtcaccgcc
     1441 agaatcccct gtggcccgac ctgtccggtc gagatcagcc aggcgatgcg ggtggtcagt
     1501 acccgcgtct tgccgctgcc ggcaccggcg aggatcaggg catgttgcgg cggcagcgtg
     1561 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaccaga ggtgatcgag
     1681 aaaaccctga agggcatcgc gattccctcc agaccccagg tcctggtgca tctccataac
     1741 gaactcgcga aagacgaccc cgacccgcga gtcatcactc gtctgatcag cggcgatgtt
     1801 ggcctctcgg ccgccgtact gaaaacggtg aattccccgt tttttggcct gtccagaagg
     1861 atgagctcgg tcgaacaggc ggtgagcctg ctcggcatgc gatccgccac gcagatcgtt
     1921 accggtcttt tgctgaggac ggcaatcgcg ggcaaggcgg gatccctgga gcgcttctgg
     1981 gacagtgccg agaaagtggc cggcatcgcc agccacatcg ccttgtccat cccccatgga
     2041 cctcgggacg aggcctattg cttcggcctg ttccacgaca ttggcatcgc catcctgatg
     2101 cagaagtttc cggattaccg gcagactctg gcgatggcag aacagatcat cgaccggccg
     2161 atcacagccg tggaggagga gcgccacgcc accgatcatg cgacgcttgg ctatctggta
     2221 gccaagggtt ggttcctgcc cgaagtcatc tgcgatggaa ttctctacca ccatgacccg
     2281 tcggtcttcg atgaccggga ggtcaccagc tcgcaggcat tgacgttgat cgccatcaat
     2341 gccctggccg agcattttca tgacgaatac ttgcacctgc gccacaacag caactggcaa
     2401 agaatgggag atcgagcact cgatcagctg ggactgcggg aggacgagta ctgcgacttg
     2461 cgggaagatt tgacgaagat ggccggctga gcggagtcgg caaccacctg agcagcggcg
     2521 cgaccgaact ggcaagtgac taactggatg gccggaagac gccggcctcg gtgaccatca
     2581 cgtcgaggcg ttgatcgtgc ggctcagggc agatcgagtc cagtcgcgcc aactcgaagc
     2641 cgacgccgac tgcgagcgga cgaggggaga ccgaagccag cgtgcggtcg aaataacctc
     2701 cgccataacc gatgcggtag cctgcggcat cgaagccatt gaccgg
//
LOCUS       DAOSIT010000309         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32029_length_2746_cov_7.557414, whole genome shotgun sequence.
ACCESSION   DAOSIT010000309 DAOSIT010000000
VERSION     DAOSIT010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32029_length_2746_cov_7.557414"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PLL14_07940"
     CDS             complement(<1..1603)
                     /locus_tag="PLL14_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HOG03898.1"
                     /translation="MTDAILLPELISCSATREPDAVALTHGQRSISYGLLQQSLSRFA
                     GGLINLGMQRGERIGIYLEKRLETVVASFGTAAAGCVFVPLNPLLKADQVAHIMRDCN
                     VRALVTSSERLSLLAPILASCHDLRQVILVNSDRLPSGDGPLAYLHWDDLLQAGIRSG
                     HRVIDTDMAAILYTSGSTGKPKGVVLSHRNIVAGAISVASYLENSAEDTLLAALPLSF
                     DAGFSQLTTAFHAGARVVLLNYLLPQDVLKALAAEKVTGLTAVPPLYIQLSQLEWPLA
                     IGQHLRYFANTGGRMPRDTLAALRARLPNSRPYLMYGLTEAFRSTYLPPEEVDRRPDS
                     IGKAIPNAEILVMREDGSPCAANEPGELVHRGALVGMGYWNDPEKTAERYKPLPASTP
                     GRGSGLVLPEIAVFSGDSVRIDEEGFLYFIGRRDEMMKTSGYRVSPTEVEEILYATRL
                     VGECAAFGVEHPSLGQAIQVIATPAAGGSLDTAAVLAECRSRMPAYMVPANIFVRQDP
                     LPRNPNGKIDRKALASEFRAHASPSG"
     gene            complement(1596..1766)
                     /locus_tag="PLL14_07945"
     CDS             complement(1596..1766)
                     /locus_tag="PLL14_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03899.1"
                     /translation="MKKQARFGKAAYRTLVVPESGNGMHCVSAGNKSQPGNVFWLKLL
                     SAILTGRCQEYD"
     gene            2017..2331
                     /locus_tag="PLL14_07950"
     CDS             2017..2331
                     /locus_tag="PLL14_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOG03900.1"
                     /translation="MNDDIVARIEANPKYHELVRKRSSYSTIMSILMIIAYYGYILLI
                     AFNKEWLGTPLAPGMVTTIGIPLGVGVIIFTIVITNVYVRKANTEFDDMSAEVIREAS
                     KK"
     gene            2328..>2746
                     /gene="actP"
                     /locus_tag="PLL14_07955"
     CDS             2328..>2746
                     /gene="actP"
                     /locus_tag="PLL14_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765784.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOG03901.1"
                     /translation="MISKSTRNIIGLILGLLATGGVLAAGADLGNTTKQATNWVAIAM
                     FAIFVAITLGITKWAAAKTKSAADFYTAGGGITGFQNGLAIAGDYMSAASFLGISGLV
                     FANGFDGLIFSIGWLVGWPVITFLMAERLRNLGKFTFA"
BASE COUNT          531 a          789 c          880 g          546 t
ORIGIN      
        1 cacctgatgg gctcgcatgc gccctgaact cgctggccag cgccttgcgg tcaatcttgc
       61 cattcggatt gcgcggcaag gggtcctggc gtacgaagat gttcgccgga accatgtagg
      121 cgggcattcg gctacggcat tcagccagca cggccgcggt atcaagactg cctccggcgg
      181 ctggcgtggc gatcacctgg atcgcctggc cgagcgaggg gtgctcgacg ccgaaagcgg
      241 cgcattcacc gaccagcctg gtggcgtaga gaatttcctc gacctcggtc gggctgaccc
      301 ggtagccgga ggtcttcatc atttcgtcac ggcgaccgat aaagtagagg aaaccttcct
      361 catcgatacg cacgctgtcg cccgagaaca ccgcgatttc gggtagcacc aggccggagc
      421 cgcggccggg cgtgctggcc gggagtggct tgtagcgttc cgcagttttt tcagggtcgt
      481 tccagtagcc catacccacc aaggcgccac gatggacgag ttcgcccggc tcgttggcag
      541 cacagggcga gccgtcttcg cgcattacga ggatttcggc attggggatg gccttgccga
      601 tcgaatccgg tcggcggtcc acctcctccg gtggcaggta ggtcgagcgg aaggcttcgg
      661 tcagaccata catgagatac ggcctgctgt tcggcaaacg tgcgcgcaag gcagccagcg
      721 tgtcacgcgg catacgtccc ccggtgttgg cgaaatagcg cagatgttgg ccgatggcca
      781 gcggccattc cagctgcgag agctggatgt atagcggagg aaccgccgtc agcccggtga
      841 ctttctcggc agcgagggcc ttgagaacgt cttgcggcaa gaggtagttg agtagtacca
      901 cgcgcgcgcc ggcatggaag gccgtcgtga gttggctgaa gccggcatcg aaagacaatg
      961 gcagggccgc cagcagcgtg tcctcggcac tgttctcgag gtagctggcg acgctgatgg
     1021 caccggcgac gatgttgcgg tgcgagagca cgaccccttt cggtttgccg gtactgccgg
     1081 aggtataaag gatcgccgcc atgtcggtgt cgatcacccg gtggccactg cggatacccg
     1141 cctgcaggag atcgtcccag tgcaggtagg ccagtgggcc gtcgccgctg ggcagccggt
     1201 cgctattgac cagaatcacc tgtcgcagat cgtggcagga agcgagaatc ggcgccagca
     1261 gggacaggcg ttcgctggat gtcaccaaag cgcgtacgtt gcagtcgcgc atgatgtggg
     1321 cgacctggtc ggccttcagc aaggggttca gtggtacgaa tacgcagccg gcggcggcgg
     1381 tgccaaagct ggcaacaacc gtttccaggc gcttttcaag gtagatgccg attcgctcac
     1441 cccgctgcat tcccagattt atcaagccgc ctgcaaagcg ggagagcgat tgctgcaaga
     1501 ggccatagct gatcgaccgc tggccgtggg tcagcgcgac ggcgtccggc tcgcgagtgg
     1561 cagagcagct gatcaattcg gggagcagga ttgcatcagt catattcttg gcagcgaccg
     1621 gtcagtatgg cagaaagcaa ttttaaccag aagacgtttc ccggctgtga tttattgccc
     1681 gccgagacgc aatgcatgcc gttgccgctc tccggcacga ccagggtgcg gtaggctgcc
     1741 ttgccgaagc gcgcttgctt tttcaaggcg agggggggct gtatcgtcgg caggcagtta
     1801 gaggaccatt cattgatcga ccgtggtgcc acaaacaccc cgcagtgcct ttccgcaggc
     1861 cacctaaggc aaggtatgaa gcagacacgg caaggcattg ttgccttggg agttccgctt
     1921 gcggcgtaag cgggaagtaa gtaatggtca ctatgattcg tcgctagtca tgatacgtga
     1981 ctcgatattt atacattcta gaggaggaga caaaaagtga acgacgacat cgttgcacgg
     2041 attgaggcca atcccaagta ccacgaactg gtccgcaagc gctccagtta cagcaccatc
     2101 atgtccattc tgatgatcat cgcctattac ggctacatcc tgttgattgc cttcaacaag
     2161 gaatggctgg gcacgcctct ggcgccgggc atggtgacga cgatcggcat tccgctgggg
     2221 gttggtgtga tcatcttcac gatcgtcatc accaatgtct atgtccgcaa ggccaacacc
     2281 gagttcgacg acatgagtgc cgaagtcatc agggaggcca gcaagaaatg atcagcaaat
     2341 ccactcgcaa catcattggc ctgatcctcg gcctgctggc caccggtggt gttctcgccg
     2401 ccggtgccga cctcggcaat accacgaagc aggcgaccaa ctgggtggcg atcgcgatgt
     2461 tcgccatctt tgtggcgatt accttgggca tcaccaagtg ggcggcggcg aagacgaagt
     2521 cggctgccga cttctacacc gccggtggtg gcattaccgg ctttcagaac ggtctggcga
     2581 ttgccggtga ctacatgtcg gcggcgtcct tcctggggat ctccggtctg gtgttcgcca
     2641 acggcttcga cgggctgatc ttctcgatcg gctggctggt cggctggccg gtgatcacct
     2701 tcctgatggc cgagcgtctg cgcaacctgg gcaagttcac ctttgc
//
LOCUS       DAOSIT010000310         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32167_length_2737_cov_9.630127, whole genome shotgun sequence.
ACCESSION   DAOSIT010000310 DAOSIT010000000
VERSION     DAOSIT010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32167_length_2737_cov_9.630127"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLL14_07960"
     CDS             complement(<1..899)
                     /locus_tag="PLL14_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOG03902.1"
                     /translation="METIGTTGMWASFFAIVLVMLAIDLLWVGGGKEHRVSLREAATW
                     SAIWVGVSFAFAGGLWWTLDGAFGREVANARALEFVAGYLIEKSLAIDNVFVWLMLFS
                     FFAVPLELQKRVLIFGVLGAIILRTIMIFAGVWLIARFDWILYVFGAFLLVTGIKMWW
                     VADEKPDLDNNPLIRWLRNHLRLSSALEGERFFVMRQTNGKWVRYATPLFLVLILVEI
                     SDLIFAVDSIPAIFAITTDPFIVLTSNVFAILGLRAMYFLLADMADRFSLLKYGLALV
                     LVFVGSKMLLLDVIKIPVVVSLAV"
     gene            1356..>2737
                     /locus_tag="PLL14_07965"
     CDS             1356..>2737
                     /locus_tag="PLL14_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG03903.1"
                     /translation="MSSQFGLLSTRRFAPFFGTQFLGAFNDNLFKNALIVLLTFQAAS
                     WTTLTPEVLTNLAAGIFILPFFLFSATAGQLADKYDKAKLARLVKLLEIVIMGVAWLG
                     FSMHSLEVLLAALFLLGLHSTLFGPVKYAILPQQLRENELVGGNALVEAGTFVAILIG
                     TLAGGLLAGAAGHPSWVAYAGLVIAALGYLCSRGIPAAPAPVPGLVVSLNPLSETWRN
                     IGFARENRTVFLSILGISWFWLYGALFLAQFPAYAKNVLGGSETSVTLLLAVFTIGIG
                     LGSLLCERLSAGHVEIGLVPFGSIGLTLFGIDLAFASPSLLPAGAPLPLAGLLALHAT
                     WRVLFDLFALGLFGGFFIVPLYALIQLRSAPEQRARIIAANNILNALFMVCGSLAAAG
                     MLGNGISIPALFGIAALCNAAVAIYIYSLVPEFMLRFIAWLLVHSVYRLQQKGLTNIP
                     EEGAAVLVCN"
BASE COUNT          501 a          836 c          838 g          562 t
ORIGIN      
        1 accgccagcg aaaccacgac cgggatcttg atgacatcga gcagcagcat cttgctcccg
       61 acgaagacca ggaccagcgc caggccgtat ttgagcagcg agaagcggtc ggccatgtcg
      121 gcgagcagga agtacatcgc ccgcaggccg agaatcgcga agacgttcga ggtcagtacg
      181 atgaaggggt cggtggtgat cgcgaagatc gccggaatcg agtcaaccgc aaagatcagg
      241 tcggaaatct cgaccaggat cagcaccagg aacagtggtg tggcgtagcg aacccatttt
      301 ccgttggtct gacgcatgac gaagaagcgc tcgccttcga gcgcgctgct caatcgcagg
      361 tggtttcgga gccacctgat cagcgggttg ttgtccagat cgggtttttc atcggcgacc
      421 caccacatct tgatgccggt gaccagcagg aaggcgccga acacgtagag gatccagtcg
      481 aatctggcga tcagccagac gccggcgaag atcatgatcg tgcgcaggat gatcgcgccg
      541 agcacgccga agatcagcac gcgtttctgc agttcgagcg gaacggcaaa gaagctgaac
      601 aacatcagcc agacgaagac attgtcgatg gccagcgatt tctcgatcag gtagccggcg
      661 acgaactcga gcgccctggc attggcgacc tcgcggccga aggcgccgtc gagagtccac
      721 cacaggccgc cagcgaaagc aaacgaaacc ccgacccaga tggcggacca ggtggccgct
      781 tccctgaggc tgacgcgatg ttccttgccg ccgccgaccc acagcaggtc gatggccagc
      841 atgacgagga cgatggcgaa gaaactcgcc cacatccctg tggtaccaat ggtttccatg
      901 aacagatact cccgatcaga tcaagtttcc cgttctcctc gtgctggcgg cacaagaaaa
      961 aacggccctt tgtccagcga ggactgaggg ccatgattct gattgcggtg ttccgaccag
     1021 gggacctttt gccatcgcgg caaaggtctt gctcatggtt acctggcgat aaccacccgg
     1081 gcgccgggaa cctcgttcat tgaggctttc cgtcctgacg acgccgcggt tgtgagctac
     1141 tcccccttgg gacaaccgca atctagcaaa acaagaataa aatagtcaac aattatttgt
     1201 ccgggagatg ggcctgctga aatctgggga cacccccccc ttgacgcttt gacgctagaa
     1261 ttcgcacccg cagccggaat cctggcgtat cgctacgtgc cgccaggcta cccagcttgc
     1321 gcggcctgcc ttgtcactca ctttaggaag acggcatgag ttcccagttt ggtctgctca
     1381 gcacccgccg ctttgcgcct tttttcggaa cgcagtttct cggcgcgttc aacgacaacc
     1441 tgttcaagaa cgccctgatc gtgctgctga cctttcaggc cgccagttgg acgaccttga
     1501 cccccgaggt actgaccaac ctggcagcgg gaatcttcat tctgcccttc tttctgtttt
     1561 cggctaccgc cggccagttg gccgacaagt acgacaaggc gaagctggcg cggctggtga
     1621 aactgctgga aatcgtgatc atgggcgtgg cctggctcgg cttctcgatg cacagtctcg
     1681 aggtgctgct cgcggcgctg tttttgcttg gcctgcattc gacactgttc gggccggtca
     1741 agtacgcgat cctgccgcag cagttgcgcg aaaacgaact ggttggtggc aacgcgctgg
     1801 tcgaagccgg caccttcgtc gcgatcctga tcggtacact ggcaggcggt ctgctggccg
     1861 gggctgccgg gcatccgagc tgggtggcgt atgccgggct ggtgatcgcg gccctcgggt
     1921 atctgtgcag ccgcggcatc ccggcggcgc cggcaccggt tcccgggctg gtggtcagtc
     1981 tcaacccact ctccgagacg tggcgcaaca tcggcttcgc gcgcgagaac cggacggtct
     2041 ttctgtcgat tctcgggatc tcctggttct ggctctacgg cgcattgttc ctggcgcagt
     2101 tcccggctta tgcgaagaat gtgctgggtg gcagcgagac ctcggtgacg ctgttgctgg
     2161 ccgtgttcac catcggcatc ggtcttggtt cactgctctg cgagcgcctg tcggccgggc
     2221 acgtggaaat tggtctggtg cctttcggtt cgatcgggtt gacactgttc ggcattgatc
     2281 tggcgttcgc ttcgccctcc ctgttgccgg ccggcgcgcc actgcccctg gctggcctgc
     2341 tggcgctgca cgcgacctgg cgggtccttt tcgatctgtt tgcgctcggg ctgttcgggg
     2401 gcttcttcat cgttccgctg tatgccctga ttcagctgcg cagcgcaccc gagcagcggg
     2461 cacgcatcat tgccgcgaac aacattctca atgccctgtt catggtctgc ggctcgctgg
     2521 ccgccgccgg catgctcggc aacggcatct caataccggc cctgttcggc atcgccgcgc
     2581 tgtgcaacgc cgcggtggcg atttacatct acagcctggt gccggagttc atgctgcgct
     2641 tcattgcctg gctgctggtg cactccgtgt atcgtctgca gcagaagggg ctgacaaaca
     2701 tcccggagga gggggcagcg gtgctggtct gcaacca
//
LOCUS       DAOSIT010000311         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32350_length_2727_cov_9.461452, whole genome shotgun sequence.
ACCESSION   DAOSIT010000311 DAOSIT010000000
VERSION     DAOSIT010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32350_length_2727_cov_9.461452"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..496)
                     /gene="nusB"
                     /locus_tag="PLL14_07970"
     CDS             complement(2..496)
                     /gene="nusB"
                     /locus_tag="PLL14_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG03904.1"
                     /translation="MTGEPRSTPDRKAATPRSPRRRARELVLQGLYQKQVSGNDEAAI
                     EAHLRDIDDFDKADREFFAGLLRGVLSERMALETQLQACLDRPFSELSPIEACVLLAG
                     AFELGNHPQTPYRVIINESIELAKAYGGTDGHKYVNGVLDKLAARLRPAEVEAKRAAR
                     GRGR"
     gene            complement(496..1014)
                     /gene="ribH"
                     /locus_tag="PLL14_07975"
     CDS             complement(496..1014)
                     /gene="ribH"
                     /locus_tag="PLL14_07975"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOG03905.1"
                     /translation="MGTPKIPGSLLPPRARYENIYEFDASLEGDGLAIGIVMSRFNQD
                     IGEGLLSACTAELKQLGVADRSIAIASVPGALEIPLTLQTMARSGRFDALVALGAVIR
                     GETYHFEIVSNDSCRALMEVQLNSGVPIANGILTCEDDDQALARMQPKGADCARAAVE
                     MARLLRALREVS"
     gene            complement(1014..2147)
                     /gene="ribBA"
                     /locus_tag="PLL14_07980"
     CDS             complement(1014..2147)
                     /gene="ribBA"
                     /locus_tag="PLL14_07980"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOG03906.1"
                     /translation="MAPQSSSADVSPIEEIIVEMRAGRTVVLVDEEDRENEGDLILAA
                     EHVTAEAINFMARFGRGLICLTLTEGRCRQLGLTQMARDNKSPYSTAFTVSIEAAEGV
                     TTGISAQDRALTIKTAVARNARPEDIVQPGHVFPIMARPGGVLVRAGHTEAGCDLALL
                     AGLEPAAVICEILKEDGSMARLGDLVDFARAHGLKIGTITDLIHYRSRHETLIERTLS
                     KPVRSRHGQFTLHAYTDRTSNEVHLVLTKGEIRADKETLVRVHEPLSVVDFLDPDGGR
                     HTFSLELAQAALARADAGVIVLLHRPESGQDILATLREPIAAQRPAARWDPRTYGIGA
                     QILRDLGVGKMKILSSPRRMPSMTGFDLEITGHIATPSELEKL"
     gene            complement(2481..>2727)
                     /locus_tag="PLL14_07985"
     CDS             complement(2481..>2727)
                     /locus_tag="PLL14_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03907.1"
                     /translation="GLAFGLATGGGLRGLAFGLATGGGLRGLAFGFAAGGGLRGLAFG
                     LVRGTLIKRLLLLLFRGRTASGKETQQDGDDYANCDQ"
BASE COUNT          477 a          831 c          874 g          545 t
ORIGIN      
        1 ctcaacgtcc gcggccgcgg gcggcacgct tggcttcgac ctcggctggg cgcaaacggg
       61 cggccaactt gtcgagcacc ccgttgacat acttgtggcc gtccgttcca ccataagctt
      121 tggccaattc gatcgactcg ttgatgatca cccggtatgg cgtttgcggg tggttaccca
      181 actcgaaagc gccggccagc agaacgcacg cctcaatcgg cgacaattcg ctgaacggac
      241 gatcgaggca ggcttgcaac tgtgtttcca gggccatgcg ctcgctgaga acgccgcgta
      301 acagtccggc gaagaactcg cggtccgcct tgtcgaagtc gtcgatgtcg cgcagatgcg
      361 cttcgattgc cgcttcgtcg ttgccactga cctgcttctg gtagagcccc tgcaagacca
      421 gttcgcgggc tctccgacgt ggggatctgg gcgtcgccgc ttttctgtca ggtgtgctgc
      481 gtggctctcc ggtcatcacg atacctcccg gagtgcgcgc agcaggcggg ccatctcgac
      541 ggcggctcgg gcgcagtcgg cccccttggg ttgcatgcgg gcgagtgcct ggtcgtcgtc
      601 ttcgcaggtc aggatgccgt tggcgatggg tacgccgctg ttgagctgga cttccatcaa
      661 cgcccggcag gaatcgttgg agacgatctc aaaatgatag gtttcgccgc ggatgactgc
      721 accaagcgca accagagcat cgaagcggcc actgcgggcc atggtctgca gcgtcaacgg
      781 aatttccagt gctcccggga cactggcaat ggcaatgctg cggtcggcga cgccaagctg
      841 cttgagttcg gccgtgcacg cactgagcag accttcaccg atgtcctggt tgaaacggct
      901 catgacgatg ccgatcgcca gcccgtcgcc ttcaagcgaa gcgtcaaact cgtatatgtt
      961 ctcgtagcgc gccctcggag gaagaaggct tccggggatt ttcggggtgc ccatcagagt
     1021 ttttccagtt cggacggagt ggcgatgtgg ccggtaattt cgagatcgaa gccggtcatg
     1081 ctcggcatgc ggcgcgggct tgacagaatc ttcatcttgc cgacgccgag atcgcgcagg
     1141 atttgcgcac cgatgccata ggtacgggga tcccagcggg cggccggacg ctgggcggca
     1201 atcggctctc gcagtgtcgc gagaatgtcc tggccgcttt cgggacggtg caacaacacg
     1261 atcacgccgg cgtctgcgcg ggccagagcc gcttgcgcca gctccagcga aaaagtatgc
     1321 cgaccgccgt cagggtcgag gaagtcgacc actgaaagtg gttcatgcac gcgcaccagg
     1381 gtttccttgt cggcgcgaat ctcacccttg gtcaatacca ggtgcacctc gttcgaggta
     1441 cggtcggtgt aggcgtgcag ggtgaattgg ccgtggcggg aacggaccgg tttggagagc
     1501 gtacgttcga tcagcgtctc gtgtcgactc cggtagtgga tgagatcggt gatcgttccg
     1561 atcttgaggc catgcgcgcg ggcaaaatcg accagatcgc ccaggcgcgc catgctgccg
     1621 tcctccttga gaatttcgca gatcaccgca gcaggttcga gcccagcgag cagggcgaga
     1681 tcgcagccgg cttcggtgtg gcccgcacgc accagaacgc cgcctggccg ggccatgatc
     1741 gggaagacgt gtcccggctg cacgatgtct tccggccgcg cgttcctggc gactgcggtc
     1801 ttgatggtca gggcgcggtc ctgcgccgag atgccggtgg tcaccccttc ggcagcttcg
     1861 atcgaaaccg tgaaagcggt gctgtagggg ctcttgttgt cgcgcgccat ctgcgtcagg
     1921 cccagctgtc ggcagcggcc ttcggtcagc gtcaggcaga tcagaccgcg cccgaaccgc
     1981 gccatgaagt tgatggcttc ggcagtgaca tgctcggcgg ccaggatcag atcgccttcg
     2041 ttctcgcggt cctcttcatc caccaggacc acggtacgtc ccgcccgcat ttcgacaatg
     2101 atttcttcga tcggcgagac atcggcagaa ctgctctgtg gtgccatctg tacctcctct
     2161 gtgaaacgtg ggttgcgcct ggcaggttgg gtaagctact tcctggtcaa ccggaactgc
     2221 atgaattcct gctgctcgac gaatgcagga cctcgattct ctcatttccc ggccaggctt
     2281 cccatatggg cggacacatt ctccgtgaat cgatcaccca tcggcccgga tattcgtttt
     2341 atcatgttcg aaaaacaaga atgaatctgt tccattgctc aggggctgga acggtgccag
     2401 ggggcgcatc ggcacctttc gatgcttgtc gatgatagtc agtacgaagg atatcttcaa
     2461 tgtcgatgaa tgagggtttg ttattgatcg cagttggcgt agtcgtcgcc atcttgctgc
     2521 gtttccttgc cgctcgccgt tctgccgcga aacaggagga gcaagaggcg cttgatcagc
     2581 gtgccgcgga cgaggccgaa cgccaggccg cggaggccgc cgcccgccgc gaagccgaac
     2641 gccaggccgc ggaggccgcc gcccgtcgcg aggccgaacg ccaggccgcg gaggccgccg
     2701 cccgtcgcga ggccgaacgc caggccg
//
LOCUS       DAOSIT010000312         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32453_length_2720_cov_11.075047, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000312 DAOSIT010000000
VERSION     DAOSIT010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32453_length_2720_cov_11.075047"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLL14_07990"
     CDS             <1..576
                     /locus_tag="PLL14_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG03908.1"
                     /translation="SQKRIQALEAHQQRLAAQTHSKTVVAPTAEKEAQPEPVPNVRGR
                     DLAQSALAMARLEGEIAKELDDYNKRPRKKFIGTSAVEYRFAQYFEEWRTKVERVGTL
                     NYPEAARGKLYGSLVLTVTIRNDGSVAGIEINRSSGHKILDDAARRIVAMAAPFAAFP
                     PAIRHDTDVLEITRTWNFTQRDSLETRAMKR"
     gene            573..1406
                     /gene="aroE"
                     /locus_tag="PLL14_07995"
     CDS             573..1406
                     /gene="aroE"
                     /locus_tag="PLL14_07995"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOG03909.1"
                     /translation="MSDRYGVFGHPVAHSKSPAIHAAFAAQCGHDLSYEAILAPLDGF
                     ADSLCAFVAAGGRGANVTVPFKEEACRLATRLTPRAAQAGAVNTLAFDSSGMLGDNTD
                     GAGLLRDLSNNLGCRITGQRLLLLGAGGAARGVVGPLLEALPAALVIANRTAARAADL
                     AERFANRGPLRACGYPDLAGESFDLVINATSASLGGEMPCLPERVFASGSLAYDMMYG
                     RGETPFLLLARAQGAARLADGLGMLVEQAAEAFRFWRGVLPDSAPVLAMLRAVAAVPA
                     H"
     gene            1409..2104
                     /gene="mtgA"
                     /locus_tag="PLL14_08000"
     CDS             1409..2104
                     /gene="mtgA"
                     /locus_tag="PLL14_08000"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOG03910.1"
                     /translation="MRGLALWVKRLLLGCLGLLLLYQFWLFGWVLWWNWANPENTRFM
                     NIRLDELRVRDPQAQLKKQWIAYEKVSNNLKRAIIAAEDAKFVDHEGFDWEGIQHALE
                     RNQKKGRVVAGGSTISQQLAKNLFLTPAKTPWRKAEEAIITLMLETIWSKQRIFEVYL
                     NVIEWGNGVFGAEAAARHYYGVSAAQLAPEQAARLAGMVPSPRFYDRNRSAPGLARKT
                     AIILARMPAADVP"
     gene            2240..>2720
                     /locus_tag="PLL14_08005"
     CDS             2240..>2720
                     /locus_tag="PLL14_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOG03911.1"
                     /translation="MKLRTILLLLVLGAIAAFSALNWGAFNTPTTLSLGVAEVQAPLG
                     VIMLGVVAFLAAFFLVFVVYVQASALFDSRRHAQELQANRELADKAEASRFTELRSFL
                     EVELQKLASGNAGTPVDAAIVARLDRLERELLTAIEQSGNGLAACIGELEDRLERRDG
                     "
BASE COUNT          482 a          875 c          877 g          486 t
ORIGIN      
        1 tcgcaaaagc gcatccaggc gctcgaagca caccagcagc ggctggcggc acaaacgcac
       61 agcaagaccg tggtcgctcc gacggccgaa aaggaagccc agcccgaacc ggtaccgaat
      121 gttcgcggcc gcgatctggc acagagcgcg ctggcgatgg cccgcctgga aggggaaatc
      181 gccaaggaac tcgacgacta caacaagcgc ccgcgcaaga agttcatcgg caccagcgct
      241 gtggaatacc gctttgcgca gtatttcgag gagtggcgaa ccaaggtcga gcgcgtcggt
      301 acgctcaact acccggaagc ggcgaggggc aagctctacg gcagcctggt gctgaccgtg
      361 accatcagga acgacggcag cgttgccggc atcgagatca accgctcttc gggtcacaag
      421 atcctcgacg acgccgcccg ccgaatcgtc gccatggccg ctcccttcgc cgccttcccg
      481 ccggccatcc ggcacgacac cgacgtcctg gagattacgc gcacctggaa ctttacccag
      541 cgcgacagcc ttgaaaccag ggccatgaag cgatgagcga tcgctacggc gtcttcggcc
      601 acccggtggc ccacagcaag tcaccggcaa tccacgcggc gtttgctgct cagtgtggtc
      661 acgatctcag ttacgaggcg atcctggcac cgctcgacgg cttcgccgac agtctgtgcg
      721 ctttcgtcgc cgcaggcgga cgcggagcca acgtcaccgt cccgttcaag gaggaagcct
      781 gccgcctggc gacgcgcctg accccgcggg cggcgcaggc gggtgcggtc aacacgctgg
      841 ctttcgattc gtccgggatg cttggggaca ataccgacgg tgccggtctg ctgcgcgacc
      901 tgagcaacaa cctcggttgc cgtattaccg gacaacgcct gcttctgctc ggcgccggtg
      961 gcgcggcacg cggcgtcgtc gggcccttgc tcgaggccct accggcggcg ctggtcattg
     1021 ccaaccgtac tgccgcgagg gccgccgacc ttgccgagcg ctttgccaac cgcggtccgt
     1081 tgcgtgcctg cggctatccg gacctcgccg gcgaatcctt cgacctggtg atcaacgcca
     1141 cgtcggcaag cctgggtggc gaaatgccgt gcctgcccga gcgggttttc gcgtccggca
     1201 gtctggccta cgacatgatg tatgggcgtg gcgagacgcc atttcttctc ctggccaggg
     1261 cgcagggcgc ggcgcgtctg gcggacgggc tcgggatgct ggtcgagcag gcggccgagg
     1321 ccttccggtt ctggcgaggg gtgcttcccg acagcgcgcc ggtgttggcc atgctgcgcg
     1381 ccgtggccgc tgtacctgcc cactgaccat gcgcggcctt gcgctctggg tgaagcggct
     1441 gcttctcgga tgcctcggct tgctgctgct gtaccagttc tggctcttcg gctgggtgct
     1501 ttggtggaac tgggccaatc cggaaaacac acgtttcatg aatatccgcc tcgacgaact
     1561 gcgggtcagg gatccgcaag cgcaactgaa aaaacagtgg attgcttatg agaaggtgtc
     1621 gaacaacctg aagcgcgcga tcatcgccgc agaggatgcc aagttcgtcg atcatgaagg
     1681 cttcgattgg gaaggcatcc agcacgcact cgaaaggaac cagaaaaagg ggcgagtcgt
     1741 tgccggcggc tcgaccatct cgcagcagtt ggccaagaat ctcttcctga cgccggccaa
     1801 gaccccctgg cgcaaggccg aagaggcgat catcacactg atgctggaga ccatctggag
     1861 caagcagcgg atcttcgagg tttatctcaa tgtcatcgaa tggggtaacg gggtgttcgg
     1921 tgccgaagcc gcggctcgcc actattacgg cgtttccgcc gcgcagctcg caccggaaca
     1981 ggcggcgcgg ctggcgggca tggttcccag cccgcgcttt tacgaccgca accgcagtgc
     2041 accggggctt gcccgcaaga cggcgatcat tctcgcccgc atgccggctg ccgacgtacc
     2101 ctgacgattc gcgcagtcga ataggcgctc gtactccggg cgatggcggg cgatcggaaa
     2161 gcctgagata cttgccgtgt tttcctgccg ccgtgcccgc cggtcggtgg ccgatgtcaa
     2221 tccatggagt gatcaatcga tgaagcttcg cactatcctt ctgctactcg ttcttggcgc
     2281 catcgctgcc ttttccgcgc tgaactgggg cgccttcaac accccgacaa cgctttctct
     2341 gggggttgcc gaagtgcagg cgccgctcgg ggtaatcatg ctcggggtgg ttgcctttct
     2401 cgccgcattc ttcctggtgt tcgtggttta cgtacaggcc tccgcgctgt tcgactcccg
     2461 ccgtcatgct caggaactgc aggccaaccg ggagctggcc gacaaggccg aggcctcccg
     2521 ctttaccgaa ctgcgcagct ttctggaggt ggagttgcag aaactggcca gcggcaatgc
     2581 cggaacgccg gtggatgcgg cgatcgtcgc ccgactcgac cggctcgaaa gggaattgtt
     2641 gaccgccatc gagcagtcgg ggaacggtct cgccgcctgc atcggcgaac tcgaggatcg
     2701 cctggagagg agagacggga
//
LOCUS       DAOSIT010000313         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32534_length_2714_cov_9.270778, whole genome shotgun sequence.
ACCESSION   DAOSIT010000313 DAOSIT010000000
VERSION     DAOSIT010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32534_length_2714_cov_9.270778"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /gene="rpe"
                     /locus_tag="PLL14_08010"
     CDS             complement(<1..644)
                     /gene="rpe"
                     /locus_tag="PLL14_08010"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG03912.1"
                     /translation="MYVIAPSILSANFAKLGEEIVNVVAAGADWIHFDVMDNHYVPNL
                     TIGPLVCEAIRPLTQAPIDVHLMVKPVDRIVPDFARAGANIITFHPESSEHVDRTLAL
                     IHEQGCQAGLVFNPATPLDYMDYVMDKLDVVLLMSVNPGFGGQKFIPATLGKLKAART
                     RIDAYARASGRQIRLEVDGGVKVDNIAEIARAGADAFVAGSAVFGAARDSDRNR"
     gene            847..>1226
                     /gene="apaG"
                     /locus_tag="PLL14_08015"
     CDS             847..>1226
                     /gene="apaG"
                     /locus_tag="PLL14_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOG03913.1"
                     /translation="MAESKKYEIAVSAVPQYIAEQSDPTNDNYVFAYTITIDNVGTVA
                     AQLISRHWIITDAGGEVQEVRGLGVVGRQPLLQPGEQFVYTSGCQLDTPVGTMRGSYQ
                     LTAVDGKQFEADIPEFTLAVPHVL"
     assembly_gap    1227..1326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1420..2658)
                     /gene="argJ"
                     /locus_tag="PLL14_08020"
     CDS             complement(1420..2658)
                     /gene="argJ"
                     /locus_tag="PLL14_08020"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOG03914.1"
                     /translation="MPVNYSTPSPAALFPVAGVRLGVTEAAIRKPDRPDLTVLDLSAG
                     TRVAGVFTANRFCAAPVQVCRRHLELGSDIRALVINTGVANAGTGDAGWHAAVDSCTA
                     LGQLLGIDDRQVLPFSTGVILEPLPTERLIAALPACVADLRVGNWHAAAHAIMTTDTV
                     AKAASRRVLIAGRTVTVTGISKGAGMIRPNMATMLGFIATDADIGTPLLQELLREAAD
                     ASFNCITVDGDTSTNDSLILIATAQAGVAFADSAAPGYAALRDTVIAVARELAQAIVR
                     DGEGASKFISIAVEGGRDRAECKRVAYAVAHSPLVKTAFFASDPNLGRILAAIGYADV
                     DDLDVDRISVWLSSVGTEVLVAEQGGRAASYREEDGAAIMQQAEIGVRIELRRGPASA
                     VVYTCDLSYDYVRINADYRS"
BASE COUNT          482 a          832 c          861 g          439 t
ORIGIN      
        1 tagcgattac ggtcgctgtc cctggcggcg ccgaaaactg ccgatccggc aacgaatgcg
       61 tcggcaccgg cgcgggcgat ttcagcgatg ttgtcgacct tcaccccgcc gtcgacctcc
      121 agacggattt gccgcccgct ggcgcgcgcg tacgcgtcga ttctggtacg tgccgccttg
      181 agcttcccga gcgtcgccgg tatgaacttc tggccgccaa agccggggtt gacgctcatc
      241 agcagcacca cgtcgagctt gtccatgacg tagtccatgt aatccagcgg cgtcgccgga
      301 ttgaacacca ggcccgcctg acaaccctgc tcatggatca gcgccagggt gcggtctacg
      361 tgttcggagc tctccgggtg gaaggtgatg atgttggcgc cggcccttgc gaaatcggga
      421 acgatgcggt ccaccggctt gaccatcaga tgcacgtcga tcggggcctg cgtcaggggg
      481 cggatggcct cacagaccag cgggccgatg gtcaggttcg gcacgtagtg gttgtccatc
      541 acgtcgaaat gaatccagtc cgcaccggcg gcgacgacat tcacgatctc ctccccgagc
      601 ttggcgaaat tggcggaaag gatgcttggc gcaatgacgt acatggcagg aacccctgat
      661 cggacagggt tatattctaa tgccaaaatg cgcccgcccc agagcacaag accgtcgaga
      721 gtgccgccaa agtcaccggg cgttgccgga aacaagcgaa tctgcgcccg gtggcgacaa
      781 caagcttgtc agcccacacg gtttggtgtg caatgcgggt tccgagtgtt ccgacatcga
      841 gcagacatgg ccgaaagcaa gaagtacgag atcgcagtca gcgcggtccc gcaatatatc
      901 gccgagcagt cggatccgac gaacgacaac tacgtctttg cgtataccat caccatagac
      961 aacgtcggga cagtcgctgc gcagctgatt tcacgtcact ggatcattac cgacgccggg
     1021 ggcgaggtgc aggaagtacg cggcctgggt gtcgtcggcc gacagccgtt gctgcagccg
     1081 ggcgagcagt tcgtctatac cagcggctgc caactcgaca cgccggtggg cacgatgcgc
     1141 ggcagctacc aactcacggc tgtcgacggg aagcagttcg aagccgacat ccccgagttc
     1201 accctggcgg ttccgcacgt cctgcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnagca gttcgaagcc gacatccccg agttcaccct ggcggttccg cacgtcctgc
     1381 attgattcca gcccttgagt ctcaagcaca gcatcgcgtc tagctgcgat agtcggcgtt
     1441 gatccgcacg tagtcgtagg aaagatcaca ggtgtacacg acagcgctgg ccgggccgcg
     1501 tcgcaactcg atgcggacgc cgatctcggc ctgctgcatg atcgcggccc cgtcctcttc
     1561 gcggtatgac gccgcccgcc cgccctgctc ggcgaccagg acctcggttc ccacgctgct
     1621 cagccagacg ctgatgcgat ccacgtcgag gtcatcaacg tcggcgtagc cgatcgcggc
     1681 cagaatgcgg ccgaggttgg ggtcggaagc gaagaaggcg gtcttcacca gcggcgaatg
     1741 ggccaccgcg taagccacgc gcttgcactc cgcacggtca cggccacctt cgacggcgat
     1801 actgatgaac ttgctggccc cttcgccgtc gcgaacgatc gcctgcgcga gttctcgtgc
     1861 gacggcgatc accgtgtccc gcagcgccgc gtagccgggc gcggccgagt cggcgaatgc
     1921 gacgccggcc tgcgcggtgg cgatcaggat cagggagtcg ttggttgacg tatcgccgtc
     1981 cacggtgatg cagttgaacg acgcatcggc cgcttcgcgc agcaactcct gcagcagggg
     2041 cgtgccgatg tcggcgtcgg tggcgatgaa accgagcatc gtcgccatgt ttggccgaat
     2101 catcccggcg cccttgctga tgccggtgac ggtgacggtc cggccggcga tgagcacacg
     2161 ccgcgaggcc gccttggcaa ccgtgtcggt ggtcatgatc gcgtgtgcgg cggcatgcca
     2221 gttgccgacc cggaggtcgg cgacacaggc cgggagagcg gcgatcaggc gctccgtcgg
     2281 caagggttcg aggatgacgc cggtcgaaaa gggcaacact tgccggtcgt cgatacccag
     2341 caactgcccg agagcggtac aactgtccac cgcggcatgc cagccggcat cacccgtgcc
     2401 ggcattcgcc acaccggtat tgatgaccag ggcgcggatg tcgctgccga gttccaggtg
     2461 gcggcggcaa acctggacgg gcgcggcgca gaagcggttg gcggtgaaca ccccggcgac
     2521 gcgcgtaccg gcgctcagat cgagaaccgt caggtccggg cgatcgggct tgcggattgc
     2581 cgcttcggtc accccgagac gaacaccggc gaccgggaac aaggctgcag gggagggggt
     2641 actgtagttg acgggcatgg cgacctcacg ttcggagaca ggcgagcggt atgcaggcgc
     2701 cgggccgctc gcct
//
LOCUS       DAOSIT010000314         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32554_length_2713_cov_8.808879, whole genome shotgun sequence.
ACCESSION   DAOSIT010000314 DAOSIT010000000
VERSION     DAOSIT010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32554_length_2713_cov_8.808879"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLL14_08025"
     CDS             <1..663
                     /locus_tag="PLL14_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 2, exosortase A system-associated"
                     /protein_id="HOG03915.1"
                     /translation="RALAAAGYAVLQIDLMGCGDSSGDFGDATWEVWLADVELACQWL
                     QRRVGAPLWLWGLRTGCLLANEAANRMVQPVNLLFWQPVLAGKQFLQQFLRLKMAAEL
                     LGNETKGVMERVRGQLRQGEPVEVAGYLLSPALANGLEKAELLLPHRSGRVEWMEISG
                     RPDGSLSPAALARLTQWQVAGQQARSTVVNGPAFWQTSEISECPALLESSLLALSETV
                     PA"
     gene            660..1619
                     /locus_tag="PLL14_08030"
     CDS             660..1619
                     /locus_tag="PLL14_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HOG03916.1"
                     /translation="MNIVEEALLFTCAGEALPGIIAWPAHLHAANTAAGSANPAQTGR
                     EHPASFADAAVSSGKEASCGVLVIVGGPQYRVGSHRQFLLLSRRLASEGYPVMRFDYR
                     GMGDGGGTMRSFEDVSADIGAAIDAFQYHCPSVQRIVLWGLCDAASAALLYVEATRDW
                     RVVGLALLNPWLRSEASLAQTHIKHYYGQRLLQREFWWKLCSGRMQVWHSLRGLLHSA
                     LLARRSGQTGGAVATRSFQERMAAGWRQFSGRMLLILSAEDYTAKEFLEFAAADPGWA
                     GLIEASKVRRIEITDADHTFSSRALRLAVEDATLTWLHTLPAA"
     gene            1653..>2713
                     /locus_tag="PLL14_08035"
     CDS             1653..>2713
                     /locus_tag="PLL14_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOG03917.1"
                     /translation="MRTESSGTYRVKLSRRGKARPGSSRWFRRYRWLVIAGWLLVGMV
                     VVWIMFIRSESVPATSSADAVAAHPAPTTQNVAEMPAVRLPADDAVHEALTEWWYYSG
                     HLQADSGERYSFHMAAFLRRGTLTHTVFQGSLLDHQTEKIYTEQARTAGNPSDGRPDG
                     FGFNFGNWQLQGAGAEHAAKMAGKDFALDLKLKDALQPVLHQAPGTPVAGLLDFGSAG
                     KSYYTSRPRMTAQGTLSIGGTVKAVRGDVWFDHQWGDFEAASLRWNWFALQLGDGADL
                     MLFELFDRQGAVVLRMGTYARDGTVSALGAADFAANSRGTWKSPTSGVLYPINWTISI
                     PARGLQLKVDPAQRASEFDA"
BASE COUNT          445 a          867 c          909 g          492 t
ORIGIN      
        1 cgcgcgctgg ccgcggccgg ctacgcggtg ctgcagattg acctcatggg ttgtggcgac
       61 agttccggcg attttggcga cgccacctgg gaggtctggc tggccgacgt cgaactcgcc
      121 tgtcagtggc tgcagcgacg ggttggcgcg ccgctgtggc tatggggcct gcgcaccggc
      181 tgcctgttgg ccaacgaagc tgccaaccgc atggtgcagc cagtcaatct gttgttctgg
      241 cagcctgtcc tcgcgggcaa acagtttctg cagcagttcc tacgactgaa gatggccgcc
      301 gaactcctcg gcaacgagac caaaggggtg atggaacgtg tgcgtggcca gttgcgacag
      361 ggagaaccgg tcgaagtggc aggctacctg ctgtcgccgg cgctcgccaa cggtctggag
      421 aaagcagagc ttctgctgcc ccaccgctct ggccgggttg aatggatgga gatctccggc
      481 aggcctgacg gcagcctttc gccggccgcg ttggcgcgcc tgacacaatg gcaggttgcc
      541 ggccagcagg cacgcagcac ggtcgtcaat ggtccggcgt tctggcagac cagcgaaatc
      601 agcgagtgcc cggcgctgct tgaaagcagc ctcctggcgc tatccgaaac ggtcccggca
      661 tgaacatcgt cgaggaagcg ctgctgttta cctgcgccgg cgaagcgttg ccgggcatta
      721 ttgcctggcc tgcccacctg cacgccgcaa acaccgctgc cggcagcgcc aaccccgcgc
      781 agaccggccg ggaacatcca gccagtttcg cggacgcagc cgtgagctcc ggaaaggagg
      841 ccagttgcgg cgtcctggtc attgtcggcg ggccgcaata tcgtgtcggc agccaccggc
      901 aattcctgct gctctcgcgc cgattggcaa gcgaaggtta cccggtgatg cgcttcgact
      961 atcgcggcat gggtgacggc ggcggcacga tgcgcagctt cgaggacgtt tccgcagata
     1021 tcggcgccgc catcgatgcc tttcagtacc actgcccgtc ggtgcagcgg atcgtgctct
     1081 gggggctgtg cgatgcggcc tcggcagcct tgctgtacgt cgaagcgacg cgtgactggc
     1141 gggtcgtggg tctggccttg ctgaacccct ggctacgctc ggaagcgagc ctggcgcaga
     1201 cccacatcaa gcactattat ggtcagcgtc tgctgcagcg cgagttctgg tggaagttgt
     1261 gcagcggccg catgcaggta tggcactcgc tgcgcggctt gctgcacagc gcgttgctgg
     1321 cgcgccgctc cggccagacc ggcggcgcag ttgccacgcg ctcgtttcag gagcgcatgg
     1381 ctgcaggttg gcggcagttt tccggacgga tgctgctgat tctgagcgcg gaagactata
     1441 ccgccaagga attcctggag ttcgccgctg ccgatcctgg ctgggcgggc ctcatcgagg
     1501 cgtccaaggt ccgtcggatt gagattactg atgccgacca tactttctcg tccagggcgt
     1561 tgcgtttggc ggtcgaggat gccacgctga catggctgca caccctgccg gcggcctgat
     1621 ccgcccccat ccacccgcga ggagctattg cgatgcgtac cgagtcgtcg ggcacctacc
     1681 gtgtgaaact ttcccgtcgg ggcaaggccc gccccggctc gtcgcgctgg ttccggcgct
     1741 atcgctggct ggtgatcgcc ggctggctct tggtcggaat ggtggtcgtc tggatcatgt
     1801 tcatccgctc cgagagcgtt ccggcaacgt catcggcgga cgccgtggcc gctcacccgg
     1861 cgccgaccac gcagaatgtg gcggagatgc ccgccgtcag actgcccgcg gacgacgccg
     1921 tgcatgaggc gctcaccgaa tggtggtatt acagcggcca cctgcaggcg gattccggcg
     1981 aacgttactc gtttcacatg gccgccttcc tgcgccgcgg cacgctcacc catacggtat
     2041 tccagggctc cctgctcgac catcagaccg agaagatcta caccgagcag gcgcgcaccg
     2101 cgggcaaccc ttcggacggt cgccccgacg gctttggctt caacttcggc aactggcaat
     2161 tgcaaggagc cggcgccgaa cacgcggcga aaatggcggg caaggatttc gcgctcgacc
     2221 tgaaactcaa ggacgcgctg cagccggtgc tgcaccaggc accggggacc ccggttgccg
     2281 gactgctcga tttcggcagc gccggcaaga gctattacac gtcgcgtccg cgcatgactg
     2341 cccaggggac attgagcatt ggcggaacag tcaaggcggt gcgcggagac gtgtggttcg
     2401 atcaccagtg gggcgacttc gaagccgcca gcctgcgctg gaactggttc gcgcttcagc
     2461 tcggcgatgg cgcggacctg atgcttttcg aactgttcga tcgccagggt gcggtcgttc
     2521 tgcgcatggg aacgtatgcc agggacggca cggtgagtgc gctcggggcc gctgacttcg
     2581 cggcgaactc gcgcggcacc tggaaaagcc caacctccgg ggtgctctat ccgatcaact
     2641 ggacgatctc gatccccgcc cgcggcctgc aactgaaagt cgatccggcg caacgggcaa
     2701 gcgagttcga cgc
//
LOCUS       DAOSIT010000315         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32576_length_2712_cov_10.312759, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000315 DAOSIT010000000
VERSION     DAOSIT010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32576_length_2712_cov_10.312759"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLL14_08040"
     CDS             <1..720
                     /locus_tag="PLL14_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HOG03918.1"
                     /translation="NKILRAFLKSKGVADADIMEEYTPFGHSDYQTIIAKIKKFSSEG
                     KKTAVVSTINGDSNVPFYKELGNAGIKATEVPVVAFSVGEEELRGVDTKPLVGHLASW
                     NYFMSLKNVENDKFTKLYRTWAVKQKLPNADKAVTNDPMEATYIGVYMWKQAVEKAKS
                     TDVDKVIAAMAGQTFKAPSGFTIKMDEKNHHLHKPVFIGEIKADGQFNVVWKTPGPVR
                     AQPWSPYIPGNDKKKDTPEGK"
     gene            832..2430
                     /gene="urtB"
                     /locus_tag="PLL14_08045"
     CDS             832..2430
                     /gene="urtB"
                     /locus_tag="PLL14_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HOG03919.1"
                     /translation="MRTIGLLLCGWLLSAASVQAAIDPSLLKPLASEDSDTKIMAIAA
                     LAQAAPEEALPVLKALADNALAVSGERLLIVDGERVFDAASNTEIKPAPAARETIGIN
                     NRVRRELSGALAALRLFSAEREVRWQAAQELTLSADAKMLPLLDKALADETDSAIRAR
                     LQLAHAQASLGSTDAGVRHVAVRALGESSDPNVRQLLMPLTEKRGDAWVEPDSAVRAA
                     AGTSIRAIEQRLAWADNLGRAFTGLSLGSILLLAALGLAITYGVMGVINMAHGELLMV
                     GAYSAWAMQSVFRAYLPSALDWYLLAAIPVGFLAAALVGVLMERIVIRHLYGRPLETL
                     LATWGISLFLMQVARTLFGAQNVEVANPVWMAGGIEVLPNLMLPWNRIIIVAFSIAVL
                     AAMWAILNLSRLGMFVRAVTQNRPMAGCVGVPTGRVDTLAFGLGAGIAGLGGVALSQI
                     ANVGPDMGQGYIVDSFMVVVLGGVGQLAGAVWAALGLGVFTKFLEGWTGAVVAKIVVL
                     VFIIIFIQKRPQGLFALKGRFVES"
     gene            2447..>2712
                     /locus_tag="PLL14_08050"
     CDS             2447..>2712
                     /locus_tag="PLL14_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HOG03920.1"
                     /translation="MRTPFIVRLGRSFDARSWQLVGLFAAFALVAVPVMHLLLPPENP
                     LYLSTYFITLTGKILCYAIVAVAMDLIWGYGGILSLGHGLFFALG"
BASE COUNT          471 a          854 c          892 g          495 t
ORIGIN      
        1 aacaagatcc tgcgcgcttt cctcaagtcc aagggcgtcg ccgacgccga catcatggag
       61 gaatacacgc cgttcggtca cagcgactat cagacgatca tcgccaagat caagaaattc
      121 tccagcgaag gcaagaagac ggccgtggtg tcgaccatca atggcgattc caacgtgccg
      181 ttctacaagg aactcggcaa cgccggcatc aaggcgaccg aagtgccggt ggtcgccttc
      241 tcggtcggcg aagaggaact gcgcggcgtc gataccaagc cgctggtggg tcacctcgcc
      301 tcgtggaact acttcatgtc gttgaagaac gtcgagaacg acaagttcac caagctgtac
      361 cggacctggg cggtcaagca gaaactgccg aatgccgaca aggcggtgac caatgacccg
      421 atggaagcga cttacatcgg cgtctacatg tggaagcagg cggtcgagaa ggccaagtcc
      481 accgacgtcg acaaggtgat tgcggcgatg gccggacaga ccttcaaggc accgtcgggt
      541 ttcacgatca agatggacga gaagaaccac catctgcaca agccggtgtt catcggcgag
      601 atcaaggccg acggtcagtt caacgtggtc tggaaaacgc ctggaccggt cagggcgcag
      661 ccgtggagcc cgtacattcc gggtaacgac aagaagaagg acacgcctga aggcaagtaa
      721 gcatcaaccg cgtgttcgtc gcatcggcga cgcacgcatc ccgttccgag cctgttcgac
      781 aggctcgcct ctcccggctg cgggagaggc ggcggggctt agggaggaag catgcgtacc
      841 atcggattac tgctttgcgg gtggctgctg agtgctgcga gcgtgcaggc ggcaatagac
      901 ccgtcgttgc tcaagccact ggcctccgaa gactccgata ccaagatcat ggccattgcg
      961 gccctggctc aggctgcgcc ggaggaggcg ctgccggtcc tcaaggcact cgcggacaat
     1021 gccctggcgg tttccggaga acgcctgctg atcgtcgacg gcgagcgcgt attcgacgcc
     1081 gccagcaaca ccgagatcaa accggcgccg gcggcgcgcg aaaccatcgg catcaacaat
     1141 cgtgtgcgtc gtgaactctc gggcgcactg gccgcgttgc gtctcttctc cgccgagcgc
     1201 gaggtgcgtt ggcaggccgc gcaggagctg acgctgagtg ccgacgccaa gatgctgccc
     1261 ctgctcgaca aggcgctggc cgatgaaacc gacagcgcga tcagggcgcg cctgcaactc
     1321 gcgcatgccc aggccagtct cggctcgacc gacgccggcg tccgccatgt ggcggtgcgc
     1381 gcactcggcg agagcagcga tccgaacgtt cgccaactgc tgatgccgct gaccgagaag
     1441 cgcggcgacg cctgggtcga acccgacagc gcggtgcgcg cggcggccgg aacgtcgatt
     1501 cgtgccatcg aacagcggct ggcgtgggcc gacaatctgg ggcgtgcctt taccggcctt
     1561 tcgctcggct cgatcctgct gctggcggca ctcggcctgg cgatcaccta cggtgtgatg
     1621 ggggtgatca acatggcgca cggcgaactg ctgatggtcg gcgcctattc ggcctgggcg
     1681 atgcagagcg tcttccgggc ctacctgccg agcgccctcg actggtatct gctggccgcc
     1741 atcccggtgg gattcctggc ggcggctctg gtcggcgtgc tcatggagcg gatcgtcatc
     1801 cgccatctct acgggcggcc gctggagacg ctgctcgcga cctggggcat ttccctgttc
     1861 ctgatgcagg tggcgcggac attgttcggc gcccagaacg tcgaggtcgc caacccggta
     1921 tggatggccg gcggcatcga ggtgttgccg aacctgatgc tgccctggaa ccggatcatc
     1981 atcgtcgcct tctcgatcgc cgtgctggcc gcgatgtggg cgatcctcaa cctctcgcgg
     2041 ctgggcatgt tcgtccgggc ggtcacccag aaccggccga tggcaggctg cgtcggcgtg
     2101 ccgaccgggc gcgtcgacac cctcgccttc ggtctcggtg ccggcatcgc cggcctcggt
     2161 ggcgtggccc tgtcgcagat cgccaacgtt ggcccggaca tggggcaggg gtacatcgtc
     2221 gactcgttca tggtcgtcgt gctcggtggc gtcgggcaac tcgccggcgc ggtctgggcg
     2281 gccctcggtc tcggcgtatt caccaagttc ctcgaaggct ggaccggcgc ggtggttgcc
     2341 aagatcgtcg tgctggtgtt catcatcatt ttcatccaga agcgtccgca ggggctgttc
     2401 gccctcaagg gccgcttcgt tgaatcctga gagcgggaga acgtccatga gaaccccttt
     2461 catcgtccgc ctcgggcgca gcttcgacgc gcgcagctgg cagttggtgg ggctgttcgc
     2521 tgccttcgcg ctggttgccg tgccggtcat gcacctgctg ctgccgcccg aaaacccgct
     2581 gtatctgtcg acctacttca ttaccctgac cggcaagatt ctctgctatg cgatcgtcgc
     2641 tgtggcgatg gacctgatct ggggctacgg gggcattctc tcgctgggtc acggactgtt
     2701 cttcgccctt gg
//
LOCUS       DAOSIT010000316         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32596_length_2711_cov_8.855045, whole genome shotgun sequence.
ACCESSION   DAOSIT010000316 DAOSIT010000000
VERSION     DAOSIT010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32596_length_2711_cov_8.855045"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="cas5c"
                     /locus_tag="PLL14_08055"
     CDS             complement(<1..365)
                     /gene="cas5c"
                     /locus_tag="PLL14_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOG03921.1"
                     /translation="MNDTRHKGIEFRLWARHALFTDPLTRVGGEKCSYHVPTYEALKG
                     IAKSIYWKPTFVWIIDEVRVIKPIRTQTKGTKPLVYSGGNDLAIYTFLADVEYQVRAH
                     FEWNQHRPELADDRNPGKH"
     gene            complement(362..>2711)
                     /gene="cas3"
                     /locus_tag="PLL14_08060"
     CDS             complement(362..>2711)
                     /gene="cas3"
                     /locus_tag="PLL14_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOG03922.1"
                     /translation="QGELIGLLHDLGKYSQAFQNYLKSAVGLLNQDEDEDYVDAASLK
                     GRIDHSTAGAQMIWSELSKQGGLGCLVGQVLSLCIASHHSGLIDCIAPDGEDTFGKRM
                     SKRSDRTFLDEALTAVDPEIISHARALLQSPQLLAGMQLVLAGILQSDSAAGKPVIAQ
                     QQIGLLARFLFSCLIDADRVDTADFEKPKAARNRMHGRYEEWGALAARLETHLARFEQ
                     RQPIDGLRADIAQHCRDGAARPKGLYTLTVPTGGGKTLASLRFALHHAKKWNMERVIY
                     AIPFTSIIDQNADVVRKVLEPFDAPASHGCIVLEHHGNLTPEQQGWKEKMLTENWDAP
                     VVYTTNVQVLETLFGAGTRGARRLHQLANAVIVFDEIQTLPINCVHLFNNAINFLVEQ
                     CGSTVVLCTATQPLLNRVNPDLGALRISASRELMPDIKGLFAKLKRVDVINHSKPGGW
                     AYAEIADLALEECRRAASCLVIVNTKASALALYRLCKPRQTAPVVHLSTSLCPAHRKR
                     ILRFVRRCLRWRIPVLCISTQLIEAGVDVDFGAVIRFTAGLDSIAQAAGRCNRHGARE
                     TGVVHVVKAREEELGTLTAIKVGQDKAERVLADFETDPEKYDRDRIGPKLLDWYYQNY
                     FFARAAEMAYPLPASETGRDDTLLNLLSRNESATREWLDRQAGPPPRLLYQSFMAAAK
                     AFKAIDSPTRGVIVPYGKTGRELVTDLCAAFEIDKQFDLLKRAQQYTVNVFPNMLAAL
                     QRAQAVYPVQEGIDILHLDPRYYSAEFGLVSEPLGLMETLLT"
BASE COUNT          525 a          794 c          833 g          559 t
ORIGIN      
        1 aaatgcttgc cggggttacg gtcatcggcc agttcgggac gatgttgatt ccactcgaaa
       61 tgtgcccgca cctgatactc gacgtcggca agaaatgtgt agatcgccaa gtcgttacca
      121 ccgctgtaca cgagcggctt ggtgcccttg gtctgcgtgc ggatcggctt gatgacccgc
      181 acttcgtcga tgatccagac gaatgtcggc ttccagtaga tcgacttggc gattcccttc
      241 agcgcttcgt aagtggggac gtgataggag cacttctcgc cgccgacacg cgtcagcgga
      301 tcggtaaaaa gggcgtggcg tgcccataac ctgaattcga ttcctttgtg gcgggtgtcg
      361 ttcacgtgag caaggtctcc atgaggccga ggggctcgct gacgaggccg aactcggcgc
      421 tgtagtagcg cgggtcgaga tgcaaaatgt cgatgccttc ctgcacggga taaaccgctt
      481 gcgcccgctg gagtgccgcc aacatattcg gaaaaacatt gactgtatat tgctgcgctc
      541 gcttcaagag gtcgaactgc ttatctattt cgaaggctgc gcaaaggtct gtgaccagtt
      601 cgcggcccgt cttgccgtag ggcacgatca cgccgcgggt cggtgaatcg atggccttga
      661 aggctttcgc cgcagccatg aaggattggt acaaaagacg tggcggtgga ccggcttggc
      721 gatccagcca ctctctggtg gccgactcgt ttcgcgaaag cagattcagc aacgtgtcgt
      781 cgcggcctgt ctcgcttgcc ggcaatgggt aagccatttc ggcggcgcga gcgaagaagt
      841 agttctggta gtaccaatcc agcaacttcg gcccgatgcg gtcgcggtcg tacttttccg
      901 gatcggtttc gaaatcagcc agcacgcgct cggctttgtc ctgacccacc ttgatcgcgg
      961 tcagcgtgcc aagctcttct tcgcgtgcct tcaccacatg gaccacgccg gtttcgcgag
     1021 cgccgtggcg attgcagcgc cccgccgcct gcgcgatcga atccagcccg gcggtgaagc
     1081 gtatgacggc accgaagtcg acatcgacgc cggcctcgat caactgcgtg ctgatgcaga
     1141 gaaccggaat ccgccagcgc aggcaacgcc gaacgaatcg cagaatgcgc ttgcggtgcg
     1201 ccgggcacag gctggtgctc aaatgcacca cgggtgcagt ttgacgcggc ttgcacaggc
     1261 gatagagcgc gagcgcagat gccttggtat tgacgatcac cagacaactt gcggcacggc
     1321 gacattcttc cagcgccaga tcggcgatct ccgcgtaggc ccagccgccc ggcttgctgt
     1381 gattgatcac gtccacgcgc ttgagcttgg cgaacaggcc cttgatgtcg ggcatcagtt
     1441 cgcggctggc ggaaatccgc aaggcgccaa ggtcgggatt gacccggttc agcagcggct
     1501 gtgtcgccgt gcaaagcacc actgtactgc cgcattgctc gaccagaaag ttgatggcgt
     1561 tgttgaacaa atggacgcag ttgatgggca gcgtttgaat ttcatcgaag acgatgacgg
     1621 cgttggccag ttgatgcagg cgccgcgcgc cgcgtgtgcc ggcgccaaac agggtttcga
     1681 gtacctgcac attggtggtg taaaccaccg gcgcgtccca gttctcggtc aacatttttt
     1741 ctttccagcc ttgctgttcc ggggtcaggt tgccgtgatg ttccagaacg atgcaaccat
     1801 gactggctgg cgcatcgaac ggttcgagca cctttcgcac cacgtccgcg ttctgatcga
     1861 tgatcgaggt aaaggggatg gcgtagatca cgcgctccat gttccatttc ttggcgtgat
     1921 gcagggcgaa acgcaggctg gccagcgtct tgccgccgcc ggtgggcacg gtcagggtgt
     1981 agagcccttt cgggcgtgcc gcaccatcgc ggcaatgctg ggcgatgtcg gcgcgcaagc
     2041 cgtcgatggg ctggcgttgt tcgaaccgcg ccaagtgcgt ttcgagacga gcggccaagg
     2101 caccccattc ttcgtagcgg ccgtgcatgc ggttgcgcgc cgctttgggc ttttcgaaat
     2161 ccgccgtgtc gacccggtcc gcgtcgatca ggcaactgaa caggaagcgc gccagcaagc
     2221 caatctgctg ctgcgcgatc accggcttcc cggcagccga atcggactgg agaattccgg
     2281 cgaggacaag ctgcatgccc gcgagcaact gcggcgactg caacagggct cgggcgtggg
     2341 agatgatctc cggatcgacg gcggtaagtg cttcgtcaag aaaagttcga tcgctccgct
     2401 tcgacatacg tttgccaaac gtgtcctcgc catccggcgc aatgcaatcg atcagaccgg
     2461 aatggtgaga agcgatgcac agagaaagca cctggccaac cagacaaccc aaaccgccct
     2521 gcttcgacaa ctcactccag atcatctgcg cgccggccgt cgagtggtcg attcgacctt
     2581 tgagactcgc ggcgtcgaca tagtcttcgt cttcatcctg attgagcaga ccaaccgccg
     2641 atttcaggta gttctgaaag gcttggctgt atttgccgag atcgtgcaac aggccgatca
     2701 attctccctg c
//
LOCUS       DAOSIT010000317         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32637_length_2708_cov_4.617791, whole genome shotgun sequence.
ACCESSION   DAOSIT010000317 DAOSIT010000000
VERSION     DAOSIT010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32637_length_2708_cov_4.617791"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="PLL14_08065"
     CDS             <1..2430
                     /locus_tag="PLL14_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOG03923.1"
                     /translation="AATALVAGLSTEQLAQLDTLFDEVPDHGVSRLTWLKSIPVAVKP
                     DHIRHILERLRFVRQIGIAAEAADNIHLDRYRQFVREGRVSATYMIERYATTRRQATL
                     VAFLIDIEERLTDSALEMADKLIGSMFTRAKNAQARRYATTSRDVARLMQIFGRTIDA
                     LTAAVDDGQDPLDALDDAVGWHTLLRIRAEVANIANTAHVDPLIVAGDRYATLRKFAP
                     DLLEALEFRAGKGSAKTIAAIETLREINRSGKRELPPNVPMPFRKEWQKLIVGDDGKV
                     NRRLWEIATLAHVRNKLRSGDLWVERSAGYRRFDSYLLTEPQAAPVVANLGLPPTAED
                     WLSERSRQLDWRLKKFAQRLKRDDLHGVRFADGRLQISPVRTAVPEAAEALADRLDAM
                     MPKIRITELLHEVARETGFLKAFTNLRTGEPCPNESALLATILADGTNLGLSRMAAAS
                     EGVTRDQLMWTHDAYIREETYRSALAAIINAHHRLPFSAVWGAGTTSSSDGQFFRGAK
                     RGASGGDINARYGVDHGFSFYTHNSDQRAPYHVNVISAATHEAPYVLDGLTSHGTDLN
                     ITEHYADTGGATDHVFALCAMLGFRFCPRLRDFPDRRLIPIGQPSAYPHIAPLLGKRV
                     RTEVIREQWSDVLRLVASIKSGHVAPSVMLRKLAAYERQNQLDVAVQEIGKVERTLFM
                     LDWLGNPELRRRCHAGLNNSEQRHALTQAIYTFRQGRIIDRSHEALQYRASGLNLVIA
                     AIVYWNTTYMADAVEHLRHAGEQVPPELLVHTSPIGWEHIAFSGDFLWERAAAATGRK
                     PLTLPSQVYAA"
     gene            complement(2515..>2708)
                     /locus_tag="PLL14_08070"
     CDS             complement(2515..>2708)
                     /locus_tag="PLL14_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03924.1"
                     /translation="TQFALAPTIGIVPARDVLAIVGFNFTGFRDRDFSASRNTQQGVF
                     VALKAKFDTSTLGFLGLGS"
BASE COUNT          523 a          860 c          821 g          504 t
ORIGIN      
        1 gccgccacag cgctcgtggc aggcctgagc actgagcagc tcgcacagct cgatacgttg
       61 ttcgatgagg tccccgacca tggcgtcagt cggctcacgt ggctcaagtc gatcccggtg
      121 gcggtcaagc ccgaccacat ccggcacatt ctcgagcggc tccgatttgt gcgacagatt
      181 ggcatcgcag ctgaggcggc cgacaacatc catctcgatc gatatcgcca gtttgtgcgc
      241 gaaggtcggg tttccgcgac ctatatgatc gagcgttatg ccaccacgcg tcgccaggcc
      301 acgctggtcg cattcctcat tgatatcgag gagcggctga ccgacagcgc gctggagatg
      361 gcggacaagc tgatcggcag catgttcacc cgagccaaga acgcgcaggc gcgccgttat
      421 gcgaccacgt cacgcgatgt tgcgaggctg atgcagatct tcggccgcac gattgatgct
      481 ctgacggccg ccgtcgatga tggccaagat ccgcttgatg cactcgatga cgccgtaggc
      541 tggcacacgc tgctcaggat cagagcggaa gtggccaaca tcgccaacac cgcgcatgtc
      601 gacccgctga ttgtcgccgg cgatcgctac gccaccttgc gcaagttcgc gccggacttg
      661 ctcgaagcgt tggagttccg tgctggcaaa ggcagcgcca agaccattgc cgcgatcgag
      721 acgctccgcg agatcaaccg atcaggtaaa cgcgaacttc cgccaaacgt gccgatgcct
      781 ttccgcaagg aatggcagaa gctgatcgtc ggcgacgatg gcaaggtcaa tcgtcgactt
      841 tgggagatcg cgaccttggc gcacgtcaga aacaagttgc gctcgggcga tctctgggtt
      901 gagcgctcgg cgggctatcg ccgcttcgac agttacctgc tcaccgagcc acaggctgcg
      961 cccgtggtgg ctaaccttgg tctgcctccg accgccgagg actggttgtc cgaacgcagt
     1021 cgccaactcg actggcggct caagaagttc gcgcagcgcc tcaaacgaga cgatttgcac
     1081 ggggtgcgct tcgcggacgg acgtttgcaa atttcgccgg tccgtacagc cgttcccgaa
     1141 gcggcggaag cgctggccga ccggcttgat gcgatgatgc cgaagatccg gatcaccgag
     1201 ttgttgcacg aggtcgctcg cgaaaccggg ttcctcaagg cctttaccaa tttgcgcacc
     1261 ggcgagccgt gcccgaacga aagcgcgctg ctcgctacga tcctggccga cggcaccaat
     1321 ctcggcctgt cccgcatggc ggcggcgagt gaaggcgtga cgcgcgatca gctaatgtgg
     1381 acacacgacg cctacattcg ggaggaaacc taccggtcgg cgctggctgc gatcatcaat
     1441 gcccatcatc ggttgccatt ttcggcagtg tggggggcgg gcacgacctc gagctctgac
     1501 gggcaattct tccggggtgc caagcgcggc gcatcgggcg gcgacatcaa cgcccgctac
     1561 ggcgtcgatc acggcttcag cttttacacg cacaattctg atcagcgcgc gccctatcat
     1621 gtcaatgtga tctcggcggc gacccacgaa gcgccgtacg tgctcgatgg cctgaccagt
     1681 catggcaccg atctcaacat cactgagcat tatgccgaca ccggcggcgc caccgatcac
     1741 gtgttcgccc tgtgcgcaat gctcgggttc aggttttgcc cgcggcttcg cgattttccg
     1801 gatcgacggt tgatcccgat cggccagccc tcggcctacc cgcatatcgc gccgctgctc
     1861 ggaaaacgcg tccgaaccga agtgatccgc gaacagtgga gtgacgtcct gcggcttgtc
     1921 gcctcgatca agtcgggcca tgtcgcgccg tcggtgatgc tccggaagct cgcagcctac
     1981 gaaagacaga accagctcga cgtcgcagtg caggagatcg gcaaggtcga gcgtacactg
     2041 ttcatgctcg attggctcgg aaaccccgag cttcgccggc gttgccacgc ggggctcaac
     2101 aacagcgagc agcgccatgc cctgacccag gcgatttaca ctttccgcca aggccggatc
     2161 atcgaccgca gtcacgaggc cctgcaatac cgcgcctccg ggctcaactt ggtgattgcg
     2221 gccatcgtct actggaacac gacctacatg gctgacgccg tcgagcacct ccgccacgcc
     2281 ggcgaacagg tgccgcccga attgctcgtt cacacgtcgc cgatcgggtg ggagcatatt
     2341 gccttttcgg gcgatttcct gtgggagcga gcggcggcgg caaccggtcg aaagccgctc
     2401 acactgccaa gccaggtcta cgcggcatga cgttcgcgct tcgttcttcc ttagcgttga
     2461 ttagcgtact ttccacgctc tgccctccac ttgcgcggcg cacagcattg ccggtcagga
     2521 ccccaggccg agaaatccaa gggtggaagt gtcaaatttg gccttgagag cgacaaagac
     2581 gccttgctgg gtgttgcgtg aggcagaaaa atcccggtct cgaaagcctg tgaaattgaa
     2641 tccgacaatt gcgagaacgt cgcgcgctgg cacgatcccg atcgtcggag ctagcgcaaa
     2701 ctgcgtta
//
LOCUS       DAOSIT010000318         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_32740_length_2700_cov_8.409452, whole genome shotgun sequence.
ACCESSION   DAOSIT010000318 DAOSIT010000000
VERSION     DAOSIT010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32740_length_2700_cov_8.409452"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLL14_08075"
     CDS             complement(<1..674)
                     /locus_tag="PLL14_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG03925.1"
                     /translation="MSAIETFPAALPDNGLLRFLTCGSVDDGKSTLIGRLLFDSKTIL
                     VDTLHAMKRTSERRGLDIVDLSLLTDGLQAEREQGITIDVAYRYFTTGTRKYIIADAP
                     GHEQYTRNMVTAASTADLAIILIDARKGVLTQTRRHSYLAHLLGIPQIVVAVNKMDLA
                     DYSPETFERIKADYLAFADQLGITDVRFMPMSALHGDMVVDRGEHLDWYDGPTLLEIL
                     ESTPAV"
     gene            complement(674..1594)
                     /gene="cysD"
                     /locus_tag="PLL14_08080"
     CDS             complement(674..1594)
                     /gene="cysD"
                     /locus_tag="PLL14_08080"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOG03926.1"
                     /translation="MSTATLSRVTLSHLDWLESEAIHIMREVAGQCSRPVLLFSGGKD
                     SICMLRIAEKAFRPGRFPFPLLHVDTGHNYPEVTDFRDRRAAELGERLIVRSVEDSMA
                     RGSVVLKSADEPRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIFSFRDEFG
                     QWDPKNQRPELWDLYNARSFKGENIRVFPISNWTEIDVWQYIEREHLALPSIYFAHQR
                     PLVRRGGGLLPVTAVTPARAGETIETLTVRFRTVGDITCTAPVISEADSVAKIIAETA
                     STTITERGATRLDDQTSDASMEQRKKEGYF"
     gene            complement(1662..2393)
                     /locus_tag="PLL14_08085"
     CDS             complement(1662..2393)
                     /locus_tag="PLL14_08085"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOG03927.1"
                     /translation="MSVQVNLDDPALLATVATRVIEARHLLREIADTFSPAAFASSLG
                     AEDMVLTNLIVRDRLDIEIFSLDTGRLPPETYDLIAAVKNHYGLTLRLYSPRHELVEA
                     YTHSNGINAFYESVELRKACCFVRKVEPLQRALAGRKAWITGMRGGQAATRDGLPVRS
                     FDAANGDGGLEKFNPLAAWSEREVWAYLKLNDVPYNALHDKFYPSIGCAPCTRPITPG
                     EDVRAGRWWWENPQSKECGLHVKHA"
     gene            complement(2390..>2700)
                     /locus_tag="PLL14_08090"
     CDS             complement(2390..>2700)
                     /locus_tag="PLL14_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HOG03928.1"
                     /translation="DDIDRLRIIAVNFPKFTDGRGYSIARLLRERYHFQGEIRAIGDV
                     LQDQLFFMRRCGIDAYALRADKDIEKALAGLSVFSETYQAAVDQSEPLFLRRRTEESA
                     "
BASE COUNT          437 a          791 c          944 g          528 t
ORIGIN      
        1 tgcaccgccg gcgtgctttc gaggatttcg agcagcgtcg gcccgtcgta ccagtccagg
       61 tgctcgccgc gatccaccac catgtcgccg tgcagcgccg acatcggcat gaagcgcacg
      121 tcggtgatgc ccagctggtc ggcgaacgcc agataatccg ccttgatgcg ctcgaaggtt
      181 tcgggcgagt agtctgcgag gtccatcttg ttcaccgcca cgacgatctg aggaatgccc
      241 agcaggtggg ccaggtagga atggcggcgg gtctgggtga gcacgccctt gcgggcgtcg
      301 atcaggatga tcgccaggtc ggcggtcgac gcggcggtga ccatgttgcg tgtgtattgc
      361 tcgtggcccg gcgcgtcggc gatgatgtac ttgcgtgttc cggtggtgaa gtagcggtag
      421 gcgacgtcga tggtgatccc ctgttcgcgc tcggcctgca ggccgtcggt cagcagcgac
      481 aggtcgacga tatccagccc gcgcctttcc gaagttcttt tcatcgcatg cagggtatcg
      541 acgaggatcg tcttgctgtc gaacagcagg cgaccaatca gggtgctctt gccgtcgtcg
      601 acgctgccgc aggtcagaaa acgcagcagg ccattgtcgg ggagggcagc ggggaatgtt
      661 tcgatcgcgg acatcagaaa tagccttcct tcttgcgttg ttccatcgag gcgtccgacg
      721 tctggtcgtc gaggcgggtc gccccgcgct cggtgatcgt cgtgctggcg gtctcggcga
      781 tgattttggc gacgctgtcg gcttcggaga tcaccggtgc ggtgcaggtg atgtcgccga
      841 cagtgcgaaa tcggaccgtc agggtctcga tcgtttcacc ggcacgggcg ggggtgaccg
      901 cggtgactgg cagcaggccg ccgccgcggc gcaccagcgg tcgctgatgt gcgaagtaga
      961 tcgacggcag cgccagatgt tcgcgttcga tgtactgcca gacgtcgatc tcggtccagt
     1021 tggagatcgg gaagacgcgg atgttctcgc ccttgaagct gcgcgcgtta tagaggtccc
     1081 acagttcggg gcgctggttt ttcgggtccc actggccgaa ctcgtcgcgg aacgagaaga
     1141 tgcgttcctt ggcgcgggct ttttcctcgt cgcggcgtgc gccgccgata caggcgtcga
     1201 aaccgaattc ctcgattgct tcgagcaggg tcaccgactg gtgcttgttg cgcggctcgt
     1261 cggccgactt gagtaccacg ctgccgcgcg ccatcgagtc ctcgaccgag cggacgatca
     1321 gacgctcacc gagttcggcc gcgcgtcggt cgcggaagtc ggtgacctcc gggtagttgt
     1381 gtccggtatc gacgtgcagc agcgggaagg ggaagcggcc tgggcggaat gccttttcag
     1441 cgatgcgcag catgcagatc gagtccttgc cgccggagaa gagcaggacc ggacggctgc
     1501 actggccggc cacctcgcgc atgatgtgga tggcctcgga ctccagccag tcgaggtggg
     1561 agagggtgac tctggaaagt gttgcagtgc tcatggggtg acctgtaatt ccgggttttc
     1621 agcgcccgtg acgtgcctgt cgcagatgtt cgtccgggcc ttcaggcgtg cttgacgtgc
     1681 aagccgcatt ccttggattg tggattttcc caccaccagc ggccagcacg cacatcctcg
     1741 ccgggcgtga tcggccgtgt acacggtgcg cagcctatgc tggggtagaa cttgtcgtgc
     1801 agtgcattgt agggaacgtc gttgagtttc aggtaagccc agacttcgcg ttcgctccat
     1861 gcggcgagcg ggttgaattt ctccaggccg ccatcaccat tggcggcgtc gaaactgcgc
     1921 accggcagcc cgtcgcgggt tgccgcctgg ccgccgcgca tgccggtgat ccaggccttg
     1981 cggccagcca gcgcgcgctg caacggctcg accttgcgca cgaaacagca ggccttgcgc
     2041 aattcgaccg attcgtagaa ggcattgatg ccgttgctgt gggtgtaggc ctcgacgagt
     2101 tcgtggcgcg gggagtacag gcgcagcgtc agtccatagt ggttcttcac cgcggcgatc
     2161 aggtcgtagg tttccggtgg caggcgaccg gtgtcgagcg agaagatctc gatgtcgagc
     2221 cggtcgcgga cgatcaaatt ggtgagcacc atgtcttcgg cgccgagact gctggcgaac
     2281 gccgccggcg agaaggtgtc ggcgatctcg cgcagcaggt gacgcgcctc aatgacgcgg
     2341 gtagccacgg tggcgagcag cgccgggtcg tcgagattga cctggacgct catgccgact
     2401 cctccgtgcg gcggcgcagg aacagaggct cgctctggtc gaccgccgcc tggtaggttt
     2461 cgctgaacac ggagaggccg gccagcgcct tctcgatgtc cttgtccgcg cgcagcgcgt
     2521 aggcgtcgat gccgcagcgc cgcataaaga agagctggtc ctgcagcacg tcgccgatgg
     2581 cgcggatctc gccctggaag tgatagcgct cgcgcagcag gcgggcgatc gagtagccgc
     2641 ggccatcggt aaacttggga aaattgacgg cgatgatgcg caaccggtcg atgtcgtcgg
//
LOCUS       DAOSIT010000319         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33010_length_2684_cov_7.014835, whole genome shotgun sequence.
ACCESSION   DAOSIT010000319 DAOSIT010000000
VERSION     DAOSIT010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33010_length_2684_cov_7.014835"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2684)
                     /locus_tag="PLL14_08095"
     CDS             complement(<1..>2684)
                     /locus_tag="PLL14_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG03929.1"
                     /translation="LNGILHCAGVLRDGYLRHKSAADLRAVLAPKVRGLVNLDQASDG
                     VGLDLFVAFSSLAGVTGNVGQADYAAANAFMDRYAAGHARLLSINWPLWADGGMRVDA
                     ATADVLRESMGLTPLATQDGLRAFYQAVAGGARQVLVAAGDSRRIDALLKPPARVAAV
                     AGDIATDATASPAAVLAELKVLFAATTRFPAAQLDAFEPLSSYGIDSIMITRLNQKLG
                     AVFGALPKTLLYEYRTLAALADYLCAHHPAACRQWRAPAGTPAPLARPAAGVSEPIAI
                     IGISGRYPQARTLAEYWDNLKAGRDCIAEIPDERWPLQGFFEPDHQLAVAQGKSYSKW
                     GGFIDGFAEFDPLFFGISPREAEGIDPQERLFIQACWEVLEDAALTRETIASRHRGRV
                     GVFAGITKSGFSLYGPELWRGGETLQPRTSFSSLANRVSYLFNLNGPSLPVDTMCSSS
                     LTAVHQACEALRHGDCELAIAGGVNLYLHPSSYVELSAAHMLSADGRCKSFGRGGDGF
                     VPGEGVGCVLLKPLARAIADGDPIHAVIRATQINHGGKTNGYTVPNPAAQRELIRATL
                     DKAGIDARSIGYVEAHGTGTEMGDPIEIAALTEAFRVDTADSGYCAIGSSKSNIGHLE
                     AAAGIAGLTRIVLQFRHRQLAPSLHAAVLNPHIDFAHSPFCVQQQLTPWQRGALPLRA
                     AISSFGAGGANAHVLLEEYVAPGTATPPPRPAAVLLSARGEAALRDRAAQLLAFLKRP
                     GAAPASALTEALRGRLAALLHVDPGLLEGAVPLEEYGVEVLHLLQLQEEFGFDGSLQD
                     CPTIDAMAQRVTPGDERVNLADLAYTLQIGREAMPFRLGCVATSVADLTARLAQFLSG
                     DASGVQLGRVSRPLEAQRELDADPARQRRAASGDLAQML"
BASE COUNT          430 a          913 c          940 g          401 t
ORIGIN      
        1 agcatctgtg ccaggtcgcc gctggcggcg cggcgctgtc gcgccgggtc ggcgtcgagt
       61 tcccgctgcg cttcgagcgg ccgcgacacc cgcccgagtt gtactccgct ggcatcgccg
      121 gacaggaact gcgcgagcct ggcagtgagg tcggcgaccg aggtggccac acatcccagc
      181 cggaagggca tcgcctcgcg cccaatctgc agcgtgtacg ccaggtccgc caggttcacg
      241 cgctcgtcgc caggcgtcac gcgctgcgcc atggcgtcga tcgtcgggca gtcctgcagg
      301 ctgccgtcga aaccgaattc ttcctgcaat tgcagcaggt gcagcacctc gacgccgtat
      361 tcttctagcg gcaccgcccc ttccagcagg ccgggatcga cgtgcaggag cgccgccagg
      421 cgtccccgca gcgcttcggt caacgccgac gcgggcgcag cgccggggcg tttcaggaag
      481 gccagcaact gcgccgcgcg gtcgcgcaag gcggcttcgc cacgcgccga cagcaacacc
      541 gcggccggcc gcggcggcgg cgtggcggtg cccggtgcga catactcttc cagcaataca
      601 tgcgcattcg cgccgccggc gccaaacgag ctgatcgccg cacgcagcgg caacgcgccg
      661 cgttgccagg gagtgagttg ctgctgcacg cagaacgggc tgtgcgcgaa atcgatatgc
      721 gggttcagga ccgccgcatg caggctgggc gccagttgcc ggtgccggaa ctgcaggacg
      781 atgcgcgtca ggccggcgat gccggcggcc gcttcgagat ggccgatgtt cgacttgctc
      841 gaaccgatcg cgcaatagcc ggagtcagcg gtgtcgacgc ggaaggcctc ggtcagtgcg
      901 gcaatctcga tcgggtcgcc catttcggta ccggtgccgt gtgcttccac gtaaccgatg
      961 ctgcgcgcgt cgatgccggc cttgtcgagc gtcgcacgaa tcagttcgcg ctgcgcagcc
     1021 ggattgggca ccgtatagcc gttggtcttg ccgccgtggt tgatctgcgt ggcgcggatc
     1081 acggcgtgga tcgggtcgcc gtcggcgatc gcacgcgcca gcggcttgag caacacgcag
     1141 ccgacgccct cgcccggaac gaaaccgtcg ccgccccggc cgaagctctt gcagcgccca
     1201 tccgcagaca gcatgtgcgc ggcgctcagt tcgacatagc tcgacggatg caggtagaga
     1261 ttgacgccgc cggcgatggc cagctcgcag tcgccgtggc gcagcgcctc gcaggcctgg
     1321 tggaccgcgg tcagcgagga cgagcacatg gtgtcgaccg gcaggctggg accgttcagg
     1381 ttgaacaggt aggaaacccg gttggccagt gagctgaagg aggtgcgcgg ttgcagcgtc
     1441 tcgccgcccc gccacaactc cggcccgtac aggctgaagc cgctcttggt aatgccggca
     1501 aacacgccga cacggccacg gtgccggctg gcgatcgtct ccctggtcag cgccgcgtct
     1561 tcgagcacct cccagcaggc ctggatgaac agtcgctcct gcgggtcgat gccctcggct
     1621 tcgcgcggcg aaatgccgaa aaacagcgga tcgaattcgg caaagccatc gatgaaacca
     1681 ccccacttgc tgtagctctt gccttgcgcg accgccaact ggtggtcggg ctcgaagaaa
     1741 ccctgcagcg gccagcgctc gtcggggatc tcggcgatgc agtcgcggcc ggccttgaga
     1801 ttgtcccagt actcggccag tgtgcgggct tgcggatagc gtccgctgat gccgatgatg
     1861 gcgatcggct cgctgacgcc ggccgcaggc ctggcgagcg gtgccggcgt gccggccggc
     1921 gcgcgccatt gtcggcacgc cgctggatga tgcgcgcaca gatagtcggc cagcgcggcc
     1981 agcgtccggt attcgtagag cagggtcttg ggcaaggcgc cgaagacggc gcccagcttc
     2041 tggttcaggc gcgtgatcat gatcgagtcg atgccgtagc ttgacagcgg ctcgaacgcg
     2101 tcgagttgcg cggcgggaaa ccgggtggtg gcggcgaaca gtaccttgag ctcggccagg
     2161 acggccgccg gcgaagccgt cgcgtccgtg gcgatgtcgc cggcgaccgc ggcgacacgc
     2221 gccggcggct tgagcagggc gtcgatccgg cgggaatcgc cggccgccac cagcacctgt
     2281 cgcgcgccgc cggcgaccgc ctggtagaaa gcccgcagac cgtcctgcgt cgccagcggc
     2341 gtcagaccca tgctctcgcg cagcacgtcg gcggtcgccg catcgacgcg catgccgcca
     2401 tcagcccaaa gcggccagtt gatcgacaac aggcgcgcgt ggccggccgc ataacgatcc
     2461 atgaaggcat tggccgccgc atagtcggcc tggccgacat tgccggtgac gccggcgagc
     2521 gaggaaaagg cgacgaacag atcgagcccg acgccgtcac tggcctggtc gaggttgacc
     2581 agaccgcgca ccttgggcgc cagcacggca cgcaggtcgg cagcgctctt gtgccgcagg
     2641 tagccgtcac gcagcacccc ggcgcagtgc aggatgccgt tcag
//
LOCUS       DAOSIT010000320         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33075_length_2681_cov_6.523991, whole genome shotgun sequence.
ACCESSION   DAOSIT010000320 DAOSIT010000000
VERSION     DAOSIT010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33075_length_2681_cov_6.523991"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLL14_08100"
     CDS             <1..1105
                     /locus_tag="PLL14_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03930.1"
                     /translation="HWDSNNRRAFAGEIIEEEACFAVAGEERCFTNIIAPIKDDHEIR
                     GIVGLNIDITERKRAEQELQRHREHLEELVSERTAALRQAMHQLVQAEKLAALGHLVA
                     GVAHELNTPLGNARLVAGTLGEHLREFASTVESGTLRRSHLATFLARAREAVELLTRN
                     TARAADLVGQFKQVAVDQTSMRRRPFDLRQTIEELLVALRPQFRRTAHRIELDIPTAL
                     RLDSYPGPLEQVISNLIGNSLIHGFVGIDAGCIGIDAAPVDAGHIRLCYRDNGTGIPE
                     PILNRIFEPFFTTRLGCGGSGLGLYIAYNLVTGVLGGTVAVRSQPGQGTVFDLVLPMV
                     APSPTVPGQASLPPGSRQRPHEPRQSSDDATNH"
     gene            complement(1152..1835)
                     /locus_tag="PLL14_08105"
     CDS             complement(1152..1835)
                     /locus_tag="PLL14_08105"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG03931.1"
                     /translation="MDGRCTPELLIFDCDGVLVDSEPIASRVLAEALTEIGIPMTAQQ
                     AVDRYTGLSLPTVLALVAAELGRDLPADFAVRLGERDRAAFLAELRPVSGVVDVLRSL
                     PWPRCVASSGSLDKIRCNLGLTGLLAFFEPYLFSSGMVPRGKPAPDLFLLAADRMGAP
                     PARCLVIEDSEAGVQAARAAGMRVLGFCGGGHTRAGTADKLLAAGAGAVFSHMPALPD
                     LLAEQGASG"
     gene            complement(1888..2367)
                     /locus_tag="PLL14_08110"
     CDS             complement(1888..2367)
                     /locus_tag="PLL14_08110"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG03932.1"
                     /translation="MNGATDPLNMKRGEPALESLLQDLLAAQKYAVLATESGGQPYTS
                     LMAFSVSEDLRDLILLTERATHKYANLMANRRVAVFIDNRENVGADTLEAVAITALGE
                     AEEVAGDDCARLRAAYLARHPYLAGFATSPSCAVMRVRVRSYIVVRDFQEVQEWRMR"
     gene            complement(2512..>2681)
                     /locus_tag="PLL14_08115"
     CDS             complement(2512..>2681)
                     /locus_tag="PLL14_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HOG03933.1"
                     /translation="SYFSTLTPQGGSQNVIFVASGYLTQGELYRLGLLTTAFCALVFL
                     VIGTPWVLWVG"
BASE COUNT          509 a          954 c          805 g          413 t
ORIGIN      
        1 gcactgggac agcaacaatc gccgggcgtt tgccggagaa atcatcgagg aagaggcctg
       61 ttttgccgtc gccggcgagg agcgatgctt caccaacatc atcgctccga tcaaggatga
      121 tcacgagatc cgggggatcg tcggactgaa catcgacatc accgagcgca agcgcgcgga
      181 acaggaactg cagcgccatc gcgaacacct cgaggaattg gtcagcgagc gcaccgccgc
      241 gttgcgccag gccatgcacc aactggtcca ggccgagaag ctggctgccc tcggccacct
      301 ggtcgccggc gtggcccatg aactcaacac cccattgggc aacgcccgcc tcgttgccgg
      361 aacgcttggc gagcatctcc gcgagttcgc cagcacggtc gaatccggca ccctgcgccg
      421 ctcgcatctg gccacgtttc tggcacgggc ccgggaagca gtcgaactgt tgacacgcaa
      481 cacggcccgc gcggccgacc tggtcggcca gttcaagcag gtcgcagtgg accagaccag
      541 catgcgccgc cggcctttcg accttcgcca gaccatcgag gaactgctgg ttgcattgcg
      601 tccgcagttc aggcgcacgg cgcaccgcat cgaactggac atccccacgg cactgcgcct
      661 tgacagctac cccggaccac tggaacaggt gatcagcaac ctgatcggca actccctgat
      721 ccacggattc gtcggcatcg acgccggctg catcggcatc gatgcggcgc cggtggacgc
      781 cgggcacatt cgcctgtgct accgcgacaa cggcacgggc attcccgagc ccatcctgaa
      841 tcgcatcttc gagccgttct ttaccacccg gctgggttgc ggcggcagcg gcctggggct
      901 gtacatcgcc tacaatctgg tcaccggcgt gctcggcggc acggtcgcgg tgcgtagcca
      961 acccggccag ggcaccgtct tcgatctcgt cctgcccatg gttgccccgt cgcccacagt
     1021 tcccggccag gcgtcgctgc cgccgggatc ccggcagcgc ccccacgagc cgcgtcaatc
     1081 aagcgacgat gccaccaatc actgaacctc agcagtcgcc gtcgacccgc tgatcgaccc
     1141 ggcggtaatc ctcaaccgct cgcgccctgc tcggcgagca gatccggcaa cgccggcatg
     1201 tggctgaaca ccgccccggc gccagcggca agcaatttgt cggcagttcc cgcacgggtg
     1261 tggccgccgc cacaaaaacc gagcacacgc atacccgccg cgcgtgcggc ctgaaccccg
     1321 gcctcactat cttcgatgac caggcaacgc gccggcggtg cgcccatgcg gtcggccgcc
     1381 aggaggaaca aatccggggc aggtttgccg cgcggtacca tcccggaact gaacaggtac
     1441 ggctcgaaga acgccagcaa tccggtcagc ccaaggttgc agcgaatctt gtcgaggctc
     1501 ccggaagagg ccacgcatct gggccagggc aagctgcgca gcacatccac caccccggag
     1561 actggacgca actcggcgag gaaagctgca cgatcacgct cgccaaggcg gacggcgaag
     1621 tccgccggca agtcccggcc cagctccgct gccaccagcg ccaggaccgt gggcaggctg
     1681 agcccggtat aacggtcgac cgcctgttgc gcggtcatcg gaattccgat ttcggtcaag
     1741 gcttcggcga gaacgcggct ggcgatcggt tcgctatcga ccagcacacc gtcgcaatcg
     1801 aaaatcagca gctctggtgt acagcgcccg tccatcagat ctgctgccgc cgcaaccgca
     1861 gctggccacc gcatcttctc ggaatcatca gcgcatgcgc cactcctgca cctcctggaa
     1921 atccctgacg acgatgtagg acctgaccct gacgcgcatg acggcgcatg acggcgaagt
     1981 cgcgaatccc gcaaggtagg ggtggcgtgc caggtaggct gcgcgcaggc gcgcgcagtc
     2041 gtcgccggcg acctcttccg cttcgcccag ggcggtgatc gccaccgcct ccagcgtatc
     2101 ggcgccgacg ttctcgcggt tgtcgatgaa caccgccacc cgccggttgg ccatcagatt
     2161 cgcatacttg tgcgtcgcgc gctcggtgag cagaatcagg tcgcgcaagt cctcgctgac
     2221 cgaaaacgcc atcaggctgg tgtacggctg cccgccgctt tcggtcgcca gtaccgcgta
     2281 tttctgagct gcaagcagat cctgcagcag cgactccagt gccggctcgc cacgcttcat
     2341 gttcaaggga tccgtagcac cgttcatcgc ctcacccttc ggccagtgca gcagactggc
     2401 tgcctcatca gaaacccagg cagttcgcgc gtcggctgtc tgaaccgcca tgctccccgc
     2461 gcgaccgcgg acgaaatccc gatcccgtcg acagcatgcg gcacaccgta atcagccaac
     2521 ccagagcacc cacggcgtgc cgatgaccag aaacaccagc gcacaaaagg ccgtcgtcag
     2581 tagcccgagc cggtacaact ctccctgggt gaggtagccg ctggcaacga agatgacatt
     2641 ctggctgccg ccctgcggcg tcagcgtgga gaagtagctg c
//
LOCUS       DAOSIT010000321         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33236_length_2670_cov_7.319312, whole genome shotgun sequence.
ACCESSION   DAOSIT010000321 DAOSIT010000000
VERSION     DAOSIT010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33236_length_2670_cov_7.319312"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLL14_08120"
     CDS             <1..936
                     /locus_tag="PLL14_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG03934.1"
                     /translation="PLAELVKIDPKSIGVGQYQHDVSQSKLAKALNGVVEDCVNAVGV
                     DVNTASVALLTRVSGLSPTLAANIVTHRDKHGAFPNRQALRGVPRLGDKTFELAAGFL
                     RVHESDNPLDRSAVHPEAYPLVERILADIRKGIGEVIGDSRLLRTLVPEKFIDDRFGL
                     PTVRDILKELEKPGRDPRPEFRAAAFREGVESAKDLQPGMILEGVVTNVANFGAFVDI
                     GVHQDGLVHISAIADRFVKDPRTVVKAGDVVKVKVLEVDLARQRIALSMRLADALPNT
                     PKAAPAPASARARQRQQDQPQPGSAMASAFARLKR"
     gene            995..>2670
                     /locus_tag="PLL14_08125"
     CDS             995..>2670
                     /locus_tag="PLL14_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOG03935.1"
                     /translation="MSKIPGTTTSKNNKAPARSAAEPRLSRQRKPADLAADEWQRALR
                     RQFGREQKFGLENLGDDPVFSEFLVFNPESRSRYRVAIRGALPGDNSCACPDFATNNL
                     GTCKHIEFTLAQIGTRPGGKTALAHGFPGSFSELFLDYSGQRRIRLRLGRDFPSDLQP
                     AARRLFDPVNDWQLPPQHFADLPAFVDHVRAVGHELRCADDALSFVAEVRDGELRRHA
                     LAAAYPLGTDSPGLQTLLKVALYPYQREGALFAARAGRALIGDEMGLGKTVQAIAAVE
                     IFARHFAAERVLIVCPTSLKHQWEREFARFTERRATVIGGLRGVRQARYAQDDFCKIT
                     NYETLSRDLDLIQAWAPDVVIVDEAQRIKNWNTIAARALKRIDSPYAIVLTGTPLENR
                     LEELISIVQFVDQYRLGPTWRLLDEHQQRDERGRVIGYRDLHRIGETLAPILLRRRKA
                     EVLDQLPERVDNTLFMPMSQLQADHHEENRQVVARIVQRWRHTGFLSDKDQLRLHCSL
                     QNMRMACNSSFLIDHETDDGYKADELLALLDDILSDPQAKVVVFSQWLRT"
BASE COUNT          533 a          920 c          781 g          436 t
ORIGIN      
        1 ccgctggccg aactggtgaa gatcgacccc aagtccatcg gggtcggcca gtaccagcat
       61 gacgtcagcc agagcaagct ggccaaagca ctcaacggcg tggtcgagga ctgtgtgaac
      121 gcggtcgggg tagacgtcaa caccgcttcg gtagcgctcc tgacccgcgt ttccggcctc
      181 agcccgacgc ttgcggcgaa tatcgtgacc catcgcgaca agcatggcgc cttccccaac
      241 cggcaagcgc tccggggagt cccgcgtctc ggcgacaaga ctttcgagct ggccgccggt
      301 ttcctgcgcg tgcatgagag cgacaacccg cttgatcgtt cggcggtaca ccccgaagcc
      361 tacccgctcg tcgagagaat tctcgccgac atccggaaag gcatcggcga agtgatcggt
      421 gactcacgac tgctcaggac gctggtgccg gaaaaattca tcgacgacag gttcggcctg
      481 ccgaccgttc gcgacatcct caaggaactc gaaaaaccgg gccgcgaccc gcgcccggaa
      541 ttccgggccg ccgctttccg ggagggcgtc gagtccgcca aggatctgca gccggggatg
      601 atcctcgaag gcgtggtcac caacgtcgcc aacttcggcg cctttgtcga cattggcgtg
      661 caccaggacg gactggtgca tatctccgcg atcgccgaca ggttcgtcaa agatccacgc
      721 acggtcgtca aggccggcga tgtggtcaag gtcaaggtgc tcgaagtgga tctggcgcgc
      781 cagcgtattg cgttgagcat gcgtctcgcc gacgcactgc cgaacacacc aaaagccgcg
      841 cccgcaccgg cctcggccag ggcacggcag cgccagcaag accagccaca accgggcagc
      901 gccatggcca gcgcctttgc ccgcctgaaa cgctgaccgc ggcggccgcc gatagccttc
      961 cacgcgaacc tgatcaacaa cattcgacga cgccatgagc aagatacccg gaaccaccac
     1021 cagcaagaac aacaaggctc ccgcccgcag cgccgccgaa ccgcgcctct cgcgccagcg
     1081 caaacccgca gacctggctg ccgatgaatg gcaaagggcg ttgcgccggc aattcggccg
     1141 cgaacagaag ttcggcctgg agaacctcgg cgacgatccg gtgttctccg aattcctggt
     1201 gttcaacccg gaaagtcgca gccgctaccg ggtggcgatt cgcggtgcac tccccggcga
     1261 caactcctgc gcgtgtcccg actttgccac caacaatctc ggcacctgca agcacatcga
     1321 attcacgctg gcgcagatcg gtactcgtcc ggggggcaag accgcgctcg cacacggatt
     1381 cccgggatcg ttcagtgaac tctttctcga ctatagcgga cagcggcgta tccgtctccg
     1441 tctgggccgc gatttcccgt ccgatctgca gccggcggcg cggcgtctgt ttgatcctgt
     1501 caatgactgg cagttgccgc cacagcactt tgccgatctg ccagcgttcg tggaccacgt
     1561 tcgcgccgtc ggccatgaat tacgctgcgc tgacgacgcc ttgtccttcg tcgccgaagt
     1621 ccgcgacggc gaactacgcc ggcacgcgct cgccgcagcc tatccgctgg gcaccgacag
     1681 ccctggcctg caaaccctgc tcaaggtcgc gctttatccc taccaacgcg aaggcgcact
     1741 cttcgctgca cgtgccggcc gcgctctgat cggcgatgaa atgggcctgg gcaaaaccgt
     1801 acaggccatc gctgcagtgg aaatcttcgc ccgccacttc gccgcagaac gggtgctgat
     1861 cgtctgcccg acctcgctca agcatcagtg ggagcgcgag ttcgctcgct tcaccgaacg
     1921 ccgcgcaacg gtcatcggtg gcttgcgcgg cgtgcgccag gcgcgttatg cacaggacga
     1981 cttctgcaaa atcaccaact acgaaacgct gagccgcgac ctcgacctga tccaggcctg
     2041 ggcgccggac gtggtgatcg tcgacgaagc acagcggatc aagaactgga acaccattgc
     2101 cgcacgtgcg ctgaagcgta tcgacagtcc ctacgccatc gtgctgaccg gcaccccact
     2161 cgaaaaccgg ctcgaggaac tgatctcgat cgtccagttc gtcgatcagt accgcctggg
     2221 acccacctgg cgcctgctcg acgaacatca acagcgcgat gagcgcggac gcgtcatcgg
     2281 ctaccgcgat ctgcaccgga tcggcgagac actggcaccg atcctgttgc gccggcgcaa
     2341 ggccgaggta ctggaccaac tacccgaacg cgttgacaac accctgttca tgccaatgag
     2401 tcagttgcag gcagaccatc acgaagagaa ccgccaggta gtcgcccgca ttgtccaacg
     2461 ctggcgccac accggctttc tctccgacaa ggaccagttg cgtctgcact gttcgttgca
     2521 gaacatgcgc atggcgtgca acagcagctt cctgatcgac cacgagaccg acgacggcta
     2581 caaggccgac gaactgctgg ccctgctcga cgacatcctg agcgacccac aggccaaggt
     2641 cgtcgtcttc agccagtggc tgcggacgca
//
LOCUS       DAOSIT010000322         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33291_length_2667_cov_9.254977, whole genome shotgun sequence.
ACCESSION   DAOSIT010000322 DAOSIT010000000
VERSION     DAOSIT010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33291_length_2667_cov_9.254977"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLL14_08130"
     CDS             complement(<1..185)
                     /locus_tag="PLL14_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOG03936.1"
                     /translation="MDEAGSRHYPEKPARVYLFGTCLIDLFCPEAGMDAVRLLEREGI
                     EVHFPAAQTCCGQPAHS"
     gene            382..1134
                     /locus_tag="PLL14_08135"
     CDS             382..1134
                     /locus_tag="PLL14_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOG03937.1"
                     /translation="MNYSKLNLPRIPDAIAHQLEARILEGSLKPGDRLPAERELAIEL
                     GVSRPSLREALKQLVSRELLVSRHGGGTYVTDRLESSFVDPWQQLMDQHPSLQDDVFE
                     FRYLLEGSAAELAAQRATDTDLQRLGQAYARLDAAYGADDRRAQVAADVAFHLTIADS
                     AHNALFSHLVASVLRLLHEHVRRSLREMAVSPEIGRQLMSQHAAIREAIVGRQPEAAR
                     LAAQTHINFVRLRLGEAARSEKRQQQSLRHLS"
     gene            complement(1181..1627)
                     /gene="smpB"
                     /locus_tag="PLL14_08140"
     CDS             complement(1181..1627)
                     /gene="smpB"
                     /locus_tag="PLL14_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOG03938.1"
                     /translation="MSIVANKKAFHDYFIEEKLEAGIALEGWEVKAIRGGRASIKEAY
                     VVIRNGEIFLFGMHITPLLAASSHVKPDPVRTRKLLLHSRQIGKLIGQVERAGYTLLP
                     LDLHFQRGRIKVEIGLAKGKKQHDKREAEKERDWVREKARLMRVKV"
     gene            1706..2149
                     /locus_tag="PLL14_08145"
     CDS             1706..2149
                     /locus_tag="PLL14_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOG03939.1"
                     /translation="MAMVNKSVLIERSSQQMFDLVDRVEDYPQFLPWCSKTRCEFRDH
                     SKTVATLHINYRSVKSHFTTENDKECPFWMRITLVDGPFRRLDGLWRFKPLAEHACKI
                     EFQLSYEFSSKMFEKVIGPVFSQIANTFVDAFVRRANEVHGLPNV"
     gene            2142..2462
                     /locus_tag="PLL14_08150"
     CDS             2142..2462
                     /locus_tag="PLL14_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOG03940.1"
                     /translation="MSEMLAIEVIYALPTKQQLVRLQLPTGSTVRQAIEASGLLQKHP
                     EIDLTRNKIGIFAKLGKLDTVLRDRDRVEIYRPLIADPKEVRKQRAAEGKVMKKGAGD
                     SEGE"
     gene            complement(2470..>2667)
                     /locus_tag="PLL14_08155"
     CDS             complement(2470..>2667)
                     /locus_tag="PLL14_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOG03941.1"
                     /translation="KAEKEQRLTAQRQEDCDAARRSLQAMESGERVAMRDARGERYFL
                     DDSQRQQEIARIRQTVQSNCK"
BASE COUNT          504 a          847 c          810 g          506 t
ORIGIN      
        1 ctgctatgcg ccggttgccc acagcaggtc tgagcggcgg gaaaatgcac ctcgatcccc
       61 tcgcgctcga gcagacggac ggcatccatg ccggcctccg gacagaagag atcgatcagg
      121 caagtcccga agaggtaaac cctcgctggc ttttccgggt agtggcggct gcctgcttcg
      181 tccatggctg actcctcttg tctcggttgg tggatggttg gcggagaagt ggtaagacca
      241 attaccggga acaataagtc ggtcggtgcg ctttgtcaat ctcccggcaa ggacccttgc
      301 gtattggtcg gcctgcccag gaaactggta agaccggccg actcctgcaa taatccaggc
      361 agacgacacc gggccacccg catgaattac agcaagctca acctgccgcg cattccggac
      421 gccatcgctc atcaactgga ggcgcgcatt cttgagggct cactgaagcc tggcgaccgc
      481 ctgccggccg aacgcgaact ggcgatcgaa ctgggggtct cgcgcccttc cctgcgcgaa
      541 gcgctcaaac aactggtctc ccgggaactg ctggtcagtc gtcacggcgg cgggacttac
      601 gtcaccgacc gactggaaag cagcttcgtc gatccctggc aacagttgat ggatcagcat
      661 ccctcgctac aggacgacgt gttcgagttc cgctatctgc tggaggggtc ggcagccgaa
      721 ctggccgcgc aaagggccac cgacaccgat ctgcagcgtc tgggacaggc gtatgcgcgt
      781 cttgacgccg cctatggtgc cgacgaccgg cgtgcgcagg tggcggctga cgtcgccttt
      841 cacctgacca tcgccgattc ggcgcacaac gccctgttca gtcatctcgt ggcgagcgtg
      901 ctgcgcctgt tgcacgagca tgtacggcgc agtctgcggg aaatggcggt gagtccggaa
      961 atcggtcgcc agttgatgag ccagcacgcg gcgatacgcg aggcgatcgt cggtcgccaa
     1021 cccgaggccg cccggctggc tgcgcagaca cacatcaact tcgtgcgtct gcgcctcggc
     1081 gaagccgcac gctcggagaa aagacagcag caatcgttgc ggcacctgag ctgacgactg
     1141 cacgaccgca cgccatgccg ctgccgctcc gcaggcgcag ctagaccttg acccgcatca
     1201 ggcgcgcctt ctcacgcacc cagtcgcgct ccttctcggc ctcgcgcttg tcgtgttgtt
     1261 tcttgccctt ggcaagaccg atctcgacct tgatgcggcc gcgctggaag tgcaggtcca
     1321 gcggcagcag ggtgtaaccg gcacgttcga cctggccgat cagcttgccg atctggcggc
     1381 tgtgcagcag caacttgcgg gtgcggaccg ggtcgggttt gacgtgcgac gacgcggcca
     1441 ggagcggcgt gatatgcatc ccgaagagaa aaatctcgcc attgcggatg accacgtacg
     1501 cttccttgat gctcgcccgg ccgccgcgga tcgccttcac ctcccaaccc tccagggcga
     1561 tgcccgcttc cagcttctct tctatgaaat agtcatgaaa ggccttcttg ttggcgacga
     1621 tgctcatgat gccgtttctc aggtgatcag ggttcgggag tgctagaatc ggcgattcta
     1681 caggattcac gggagatggc agaggatggc catggtcaac aagtcggtgc tgatcgaacg
     1741 ttcatcgcaa cagatgttcg atctggtcga ccgtgtcgag gactatccgc agttcctccc
     1801 ctggtgcagc aagacccgct gtgaattccg tgaccacagc aagacggtcg ccacgctgca
     1861 catcaactac cgcagcgtca agtcgcattt caccaccgag aacgacaagg aatgcccgtt
     1921 ctggatgcgc atcacgctgg tggatggtcc gtttcgccgg ctcgacggtt tatggcgttt
     1981 caagcctctg gccgaacacg cctgcaagat cgaattccag ctctcctacg agttctccag
     2041 caagatgttc gagaaggtga tcggcccggt cttcagccag atcgccaaca ccttcgtcga
     2101 cgccttcgtg aggcgtgcga acgaggtcca tgggctgccc aatgtctgag atgctggcga
     2161 tcgaagtgat ctacgccctg ccgacgaagc agcagctggt gcgcttgcag ttgccgacgg
     2221 gcagcaccgt acggcaggcc attgaagctt ccggactgct gcagaagcac ccggaaatcg
     2281 atctgaccag gaacaagatc ggtattttcg ccaagctcgg caaactcgac accgtcctgc
     2341 gcgatcgcga ccgcgtggaa atctaccgcc ccctgatcgc cgacccgaag gaagtacgca
     2401 agcagcgcgc ggccgaaggc aaggtcatga aaaagggcgc cggcgacagc gagggcgaat
     2461 aggccgcgct cacttgcagt tgctctgcac ggtctggcgg attctcgcga tctcctgttg
     2521 gcgctggctg tcgtccagga agtagcgctc accccgggcg tcacgcatcg ccacgcgttc
     2581 tccggattcc atggcctgca gcgagcgccg ggcggcgtcg cagtcctcct gtctctgggc
     2641 agtcagccgc tgctcctttt ccgcctt
//
LOCUS       DAOSIT010000323         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33293_length_2667_cov_8.543645, whole genome shotgun sequence.
ACCESSION   DAOSIT010000323 DAOSIT010000000
VERSION     DAOSIT010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33293_length_2667_cov_8.543645"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="secE"
                     /locus_tag="PLL14_08160"
     CDS             <1..344
                     /gene="secE"
                     /locus_tag="PLL14_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOG03942.1"
                     /translation="FKFALAVLLLVAGVAGFYLLAEQAMILRVLAVLAGASLAAAAAW
                     QTEQGRRFFDFAREASAEARKVVWPSRKETVQTTGLVFAFVVVMAVFLWLTDKSLEWL
                     LYDLVLGWRKL"
     gene            341..874
                     /gene="nusG"
                     /locus_tag="PLL14_08165"
     CDS             341..874
                     /gene="nusG"
                     /locus_tag="PLL14_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOG03943.1"
                     /translation="MSMRWYVVHAYSGFEKSVQRALAERIQRQSMQHAFGRILVPVEE
                     VVELKMGQKSISERKFFPGYVLVEMEMNDDSWHLVKSTPKVTGFVGGTANKPTPISEK
                     EVDKIMQQMQDGVDKPRPKFLFEPGEVVRVKEGPFADFHGAVEQVNYEKNRLRVSVTI
                     FGRPTPVELEFGQVEKA"
     gene            1000..>1391
                     /gene="rplK"
                     /locus_tag="PLL14_08170"
     CDS             1000..>1391
                     /gene="rplK"
                     /locus_tag="PLL14_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOG03944.1"
                     /translation="MAKKIIGYIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKTFNA
                     QTQGLEPGLPIPVVITAFADKSFTFIMKTPPATVLIKKAIGIQKGSAKPHTAKVGVLT
                     RAQCEAIATQKMPDLTAADMEAAVRTIIG"
     assembly_gap    1392..1491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1492..1971
                     /gene="rplA"
                     /locus_tag="PLL14_08175"
     CDS             <1492..1971
                     /gene="rplA"
                     /locus_tag="PLL14_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOG03945.1"
                     /translation="KTIRVAVFAQGEKAAAAKAAGADIVGFEDLAAEIKSGVINFDRV
                     IATPDAMRVVGQLGQILGPRGLMPNPKVGTVTMDVVTAVQNAKAGQVQYRTDKGGIIH
                     STIGRASFDSDKLALNLKALLDALIKAKPATSKGQYVKRVALSSTMGPGVRVDASTL"
     gene            2261..>2667
                     /gene="rplJ"
                     /locus_tag="PLL14_08180"
     CDS             2261..>2667
                     /gene="rplJ"
                     /locus_tag="PLL14_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOG03946.1"
                     /translation="MGLNLDEKKVVVAEVSARVASAQTIVLAEYSGLPVAHLTRLRAQ
                     ARAAGVYLRVMKNTLVRRAVEGTAFAGLAEQMTGPLIYSISDDPVAAAKVLNDFAKTN
                     DKLVLKAGSFAGDLLDKAGVQALASIPSRNELLA"
BASE COUNT          518 a          675 c          838 g          536 t
ORIGIN      
        1 agttcaagtt tgcgttagcg gtactgctgc ttgtggccgg cgtcgctggc ttctatctgc
       61 tcgccgagca ggccatgatc ctgcgtgtcc tggccgtcct ggccggtgca tcgctggcgg
      121 ctgcggctgc gtggcagacc gagcaggggc gccgtttctt cgacttcgca agagaagcgt
      181 cggccgaagc gagaaaggtg gtctggcctt cacgaaaaga aaccgtgcag acaacgggcc
      241 tggttttcgc cttcgttgtg gtgatggccg tatttctctg gttgaccgac aagagccttg
      301 agtggctgct gtatgacctg gttcttggtt ggagaaagct atgagcatgc gctggtatgt
      361 ggtacacgcc tattccggtt tcgagaaaag cgtgcagcgc gccttggccg aacgtattca
      421 gcggcagtcc atgcagcatg cctttggtcg catcctggtt ccggtagaag aggttgtcga
      481 actcaagatg ggccagaaaa gcatctccga gcgcaagttc ttccctggct atgtgctggt
      541 ggaaatggag atgaacgacg attcctggca tctggtcaag agtacgccca aggtgacggg
      601 ttttgtcggt ggcacggcca acaagccgac gccgatttcc gaaaaggaag tcgataaaat
      661 catgcagcaa atgcaggatg gtgtcgataa gcctcgcccc aagtttcttt tcgaaccagg
      721 cgaagtcgtt cgcgtcaagg aagggccgtt cgccgatttc catggtgcag tcgaacaggt
      781 gaactacgaa aagaatcgtc tgcgcgtgtc ggtaacgatt ttcggtcgcc cgacaccggt
      841 ggaactggag ttcgggcaag tcgagaaggc ctgatcctga aactgggatg cggtgcgtgg
      901 tgttgtcggc gcgccgcaat agtgcggctt cctggccgcg tttgtcgttt ggggagcgct
      961 gtcgcgagcg cgtttgcacc cgcctagagg agtaatagcg tggcaaagaa gattatcggc
     1021 tatatcaagc tgcaagtgcc ggcggggaag gcgaatcctt cgcctcctat cggcccggcg
     1081 ctgggtcagc gcggtctgaa catcatggag ttctgcaaaa ccttcaacgc ccagacgcaa
     1141 gggctggaac caggtttgcc gattcctgtg gtgatcaccg ctttcgccga caagagtttt
     1201 accttcatca tgaagacgcc gccggcgacc gtgctgatca aaaaggcgat cggtattcag
     1261 aagggcagtg ccaagcctca taccgccaag gttggcgtgc tgacccgggc ccagtgcgaa
     1321 gccattgcca cccagaagat gcccgacctg acggctgcgg acatggaggc tgcggttcgg
     1381 acgatcattg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagaccatt
     1501 cgcgtggccg tcttcgcgca gggcgaaaag gctgccgcag ccaaggctgc cggtgccgat
     1561 atcgtcggct tcgaagacct tgcagccgag atcaaatccg gggtgatcaa tttcgaccgg
     1621 gtgattgcaa ccccggacgc catgcgcgtg gtcggtcaat tgggtcagat cctgggtccg
     1681 cgtggcctga tgccgaaccc gaaagtgggt acggtcacga tggatgtggt taccgcagtc
     1741 cagaatgcca aggctggaca ggtccagtac cgtaccgaca agggcgggat catccacagc
     1801 accatcggac gtgcttcatt cgacagcgac aaactggcgc tgaacctgaa ggctctgctc
     1861 gatgcgctga tcaaggccaa gccggcaacc tccaaggggc agtatgtgaa gcgggtggcc
     1921 ctgtcgagca cgatggggcc gggtgtgcgc gtggacgctt ccacactgta gcagaagagc
     1981 agcaccgggc gacgacgatg cgcgttggcc tggtaacgaa ctttgttggg ttatcggccg
     2041 ctccttactg gtgtgtcgat ggatcgtcaa agaccgcagg tgtcgaaggt tgtcgccaga
     2101 ggcttaagcg tgcaagcggc ctgcgtagac ggtgcgcccc gaaaaggatt gcgcagtaga
     2161 agagccttgt gcccctcgct gcaactcctg atcaaggtcg ccgtgggtgc gacgcctcgt
     2221 atcgatgcgt cgtgtgttga cttgatggga ggaaaggctt gtgggtctca atcttgatga
     2281 aaagaaagtc gtcgtggccg aagtctcggc cagggtggcg agtgcccaga cgatagtatt
     2341 ggctgagtac agcggtttgc cggtggccca cctgacccgt ctgcgggcgc aggcgcgtgc
     2401 cgcgggtgtt tatcttcggg tgatgaagaa caccctggtc aggcgagcgg tcgagggtac
     2461 ggcgtttgcc ggcttggccg agcagatgac cggaccgttg atttacagca tctcggatga
     2521 tccggttgcc gcggcaaagg tactcaacga ctttgcaaaa accaatgaca aactggtgct
     2581 gaaggctggc agctttgctg gcgatcttct ggacaaggcc ggtgtgcagg cgcttgcgtc
     2641 gattccaagt cgcaacgaac tgctcgc
//
LOCUS       DAOSIT010000324         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33449_length_2657_cov_10.177940, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000324 DAOSIT010000000
VERSION     DAOSIT010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33449_length_2657_cov_10.177940"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..>1367)
                     /locus_tag="PLL14_08185"
     CDS             complement(177..>1367)
                     /locus_tag="PLL14_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOG03947.1"
                     /translation="KGADPLNLFIVVETGDHHVSILDGDSFERLHRFPSRYALHGGPK
                     FSPDGRFVYFASRDGWISKFDIYNLVVVAEIRVGLNTRNAAVSSDGKWVMVANTLPHD
                     LVVLDAADLSLVKIIPTMNAAGDQSSRVAAVYDAAPRRSFVAALKDVPEVWEISYDPE
                     AEPIAEGLVHDYQYREGHFKPAMFSPRRTKLEDCLDDFFFTQSYDTILGSSRSATRGQ
                     VVLLDVRRKVADLDLPGMPHLGSGITWKWRDRSGSERTVMASPNLNAGVVTVIDLGTY
                     QTIKQIKTLGPGFFLRSHENSRYAFTDSMMSKEHQNALQVIDKQTLEVVAQITAEPGQ
                     TLAHVEFTRDGRYALASLWEQDGALVVIDAQTLKEVKRIPAKKPVGKYNVWNKISRSE
                     GTSH"
     assembly_gap    1368..1467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1536..>2657)
                     /gene="nirJ"
                     /locus_tag="PLL14_08190"
     CDS             complement(1536..>2657)
                     /gene="nirJ"
                     /locus_tag="PLL14_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HOG03948.1"
                     /translation="APVVIWNLIRRCNLTCKHCYSISTDKDFPGELTTDEILAVMDDL
                     KAFRVPVLILSGGEPLLRPDIFGVASHAKAMGFYVGLSSNGTLIDATSIDRIAACDFD
                     YVGVSLDGIGATHDRFRQMPGAFDASLRGIRLCRDLGLKIGVRFTMTQENAHDLPRLL
                     ELVEDEGIDRFYFSHLNYAGRGNNNRKDDAQHRLTRWAMDLLFDSCWHYQQRGLRKEF
                     TSGNNDADGVYFLHWVQKRFPAQASNIHARLLQWGGNSSGVNVANIDNLGNVHPDTMW
                     WHYSLGNVRQRPFSEIWMDLSDPLLAGLKQFPRRVSGRCGECRYFGICNGNTRVRAQQ
                     LTGDAWAEDPGCYLDDEEIGVAPGRERLRVTPHIKLRRV"
BASE COUNT          492 a          750 c          812 g          503 t
ORIGIN      
        1 ttcgtgctgc cggtttcggc ggggccgcgg cttcaccgaa ggtcaccgcc gcatccgcat
       61 cctcggctgc cttcggaacg gccatggtca gcaccgcgaa caaggacaag gccgcagcca
      121 gcagccagcg caacagacgg tgggggaact tgctcatggt caactttctg gccggatcaa
      181 tgactggtgc cttcggaacg cgatatttta ttccagacgt tgtacttgcc gaccggtttc
      241 ttcgccggga tgcgtttcac ttccttcagc gtctgcgcgt cgatgaccac cagcgcgccg
      301 tcctgttccc aaagactggc cagcgcgtaa cggccgtcac gcgtaaactc gacatgcgcc
      361 agcgtctgtc caggttcggc agtgatttgc gcgaccactt cgagcgtctg cttgtcgatg
      421 acctgcagag cattctggtg ttccttgctc atcatggaat cggtgaaggc ataacggctg
      481 ttttcgtggc tgcggagaaa gaacccgggt ccaagggtct tgatctgctt gatggtctga
      541 taggtaccga ggtcgatcac ggtgaccaca cccgcattga gattgggcga ggccatcacg
      601 gtccgctcgc tgcccgagcg gtcacgccac ttccaggtga ttccggagcc caggtgcggc
      661 atccccggca gatccaggtc ggcaaccttg cggcggacgt ccaggaggac cacctgtccc
      721 cgggttgccg atcgcgaaga gccgagaatc gtgtcgtaac tctgggtgaa gaagaagtca
      781 tcgagacagt cctcgagctt cgtccggcgc ggggagaaca ttgccggctt gaaatgacct
      841 tccctgtatt gatagtcatg tacgagcccc tcggcaatcg gttcggcttc ggggtcataa
      901 ctgatttccc agacctccgg cacatccttg agcgcagcca cgaaactcct gcgcggcgcg
      961 gcatcataga ccgccgcaac gcgcgaactc tggtcaccgg cggcattcat cgtcgggatg
     1021 atcttcacca gagagaggtc ggcggcatcg agtaccacca gatcatgcgg cagggtgttg
     1081 gcgaccatca cccacttgcc atcgctcgat accgcggcgt tgcgcgtatt gaggccaacg
     1141 cggatctcgg cgacgacgac caggttgtag atgtcgaatt tgctgatcca gccgtcgcgg
     1201 gaggcgaagt agacgaagcg accgtccggt gagaattttg gtccgccatg cagcgcatag
     1261 cgactgggaa agcgatgcag ccgctcgaag ctgtcgccgt cgaggatcga cacatgatgg
     1321 tcgccggttt cgacgacgat gaacaggttg agcgggtcgg cacctttnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnggc cagggatgac ccggcggcaa gaccggccgc
     1501 caggccgacg acagcaaggc cagagcgcca gttcatcaga ccctccgcag tttgatgtgc
     1561 ggcgtcaccc gcaggcgctc gcgaccgggg gcgacgccga tttcctcgtc gtcgaggtag
     1621 cagcctgggt cttccgccca ggcgtcgccg gtcagttgct gggcacgcac gcgggtattg
     1681 ccgttacaga ttccgaagta ccggcattcc ccacagcgtc ctgaaacgcg gcgtggaaac
     1741 tgcttcaggc cggccagtag cgggtcggat agatccatcc agatttccga aaatggacgc
     1801 tgacgcacgt tgccgagact gtagtgccac cacatggtat cggggtggac gttgccgaga
     1861 ttgtcgatat tggccacgtt gactcccgat gaattaccac cccactgaag caggcgagcg
     1921 tggatgttgg atgcctgggc gggaaatcgc ttctgtaccc agtgcagaaa gtagacgccg
     1981 tcggcatcgt tgttgccgct ggtgaactcc ttgcgcagac cgcgctgctg gtagtgccag
     2041 cagctatcga agagcagatc catcgcccag cgcgtcagtc ggtgctgtgc gtcatccttg
     2101 cggttgttgt tgccgcgccc ggcatagttg aggtgcgaga aatagaagcg gtcgatccct
     2161 tcgtcctcga ccagttcgag caggcgtggc aggtcgtggg cattttcctg cgtcatcgtg
     2221 aaacgaaccc cgattttcag tccgaggtcg cggcagaggc ggatgccgcg cagcgaggcg
     2281 tcgaacgccc ccggcatctg gcgaaatctg tcgtgggttg caccgatgcc atcgagtgaa
     2341 acgccgacat agtcgaaatc gcatgcggcg atgcggtcga tagaggtggc gtcgatcagc
     2401 gtgccgttcg aagacaggcc gacgtaaaag cccatcgcct tggcgtggct ggcgacgccg
     2461 aagatgtctg gtcgtagcag tggctctccg ccggagagga tcaggaccgg gacgcgaaag
     2521 gctttgagat cgtccatcac ggcaaggatt tcgtcggtgg tcagttcacc ggggaagtcc
     2581 ttgtcggtgg aaatcgagta gcaatgcttg caggtcagat tgcagcgccg gatcaggttc
     2641 cagatcacca ccggcgc
//
LOCUS       DAOSIT010000325         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33566_length_2650_cov_8.204239, whole genome shotgun sequence.
ACCESSION   DAOSIT010000325 DAOSIT010000000
VERSION     DAOSIT010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33566_length_2650_cov_8.204239"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLL14_08195"
     CDS             complement(<1..890)
                     /locus_tag="PLL14_08195"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOG03949.1"
                     /translation="MYSGLVDLPWWADVLITLGLTHVSIAAVTIFLHRHQAHRALDLH
                     PLPAHFFRLWLWLATGMVTKEWAAIHRKHHAKCETPEDPHSPQIFGIHRVLWTGVFLY
                     VREARNAETLRRYGHGTPDDWIENHLYTPWHKLGVLIMLAIDMALFGVLPGFLIWAVQ
                     IIWIPFWAAGVINGLGHFWGYRNFASPDASTNISPWGILIGGEELHNNHHTYPTSARL
                     SIHWYEVDIGWLYIRILAALGMARVKKVAPRPRLGTLRPVVDFDTLQAVIVNRYDVLS
                     QYARSLKQVYREELATLQDS"
     gene            1070..1789
                     /locus_tag="PLL14_08200"
     CDS             1070..1789
                     /locus_tag="PLL14_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOG03950.1"
                     /translation="MKTRILLVEDDERLAKLTAEYLHKNDFEVLVEANGNKAETRILD
                     ERPDLVILDIMLPGKDGFEICRAVRAQYRGVILMLTARDEDFDQILGLELGADDYITK
                     PVQPRLLLARIKALLRRAPGNPVVADESAEPSEPAELSFGRFRISQATRSTQLGDDGI
                     DLTTAEFDLLWLLARHAGSILSRDDLLQELRGIGFDGLDRSIDARISRLRRKLGDDPE
                     NPTRIKTVRGKGYLFSKHDWQ"
     gene            1776..>2650
                     /locus_tag="PLL14_08205"
     CDS             1776..>2650
                     /locus_tag="PLL14_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOG03951.1"
                     /translation="MTGNNKLLAILGNYKPAPWRGRYSLSRLFFNFYLLVMGSFVAIA
                     IFADFVISTAVKGITDDYTSRFMRGTVLLIEEELFRKPRSEWPRTIEALDRKFAYKLE
                     IVDRWTLNLPAAQAEKLDGGELAIDAQGDIIYHRLKQTPQILVVGPISPERNPEYRRG
                     MPLDLRISLLTWSLIGVCLAIVVWLWVHPVWRDLEAMRQTARALGEGLFEARAPIMRS
                     SAFGPLAETLNGMAERIQRLIATQKELSSAISHELRTPIARLRFALEMLAETHDAAEG
                     ERLSRMMESDLDELDG"
BASE COUNT          535 a          841 c          813 g          461 t
ORIGIN      
        1 tggctgtcct gcagcgtggc gagttcctcg cgatacacct gcttcagcga cctggcgtat
       61 tgcgacaata cgtcatagcg gttgacgatc accgcctgca gggtatcgaa gtcgacgacc
      121 gggcgcagcg tccccaggcg cggcctgggc gccaccttct tcacccgcgc catgcccagc
      181 gcggcaagaa tgcggatata cagccagccg atgtcaactt cgtaccagtg gatcgacaac
      241 ctggcggaag taggataggt gtgatggttg ttgtgcagtt cctcgccgcc aatgaggatg
      301 ccccaggggg aaatgttggt gctggcgtcc ggggaagcga agttccggta gccccagaaa
      361 tgtccgagcc cgttgatcac gcccgccgcc cagaagggga tccagatgat ctgcacggcc
      421 cagatcagaa aacctggcag cacgccgaac aaggccatgt caatggccag catgatcagc
      481 accccgagct tgtgccacgg cgtgtagagg tgattctcga tccagtcgtc cggtgttcca
      541 tgcccgtatc ggcgcagggt ttcagcgttc ctggcttccc tgacgtagag gaagacaccg
      601 gtccacagga cgcggtggat gccgaagatc tgcgggctgt gtgggtcctc gggggtctcg
      661 catttggcgt gatgcttgcg gtgaatcgcg gcccattcct tggtcaccat gccggtcgcc
      721 aaccacagcc acaagcgaaa gaagtgcgcg ggcagcgggt gcaggtcgag cgcgcgatgc
      781 gcctgatgcc ggtgcaggaa gatggtcacc gcggcaatgc tcacatgggt caggcccaga
      841 gtaatcaata catcagccca ccagggcaaa tcgacgagtc cagagtacat ggaacgttcc
      901 aggaaagagc aaggccgcga ttctaaggca ataacacgcg cgcgccaaac tccccgatga
      961 accggaacag cgtgccggcg caagtcgcgc gccagtccca tggcagcaca tttggcatct
     1021 tattgggaac ggacgttcac acgtgccgac aacccgttat gattgaatga tgaagacacg
     1081 tatcctgctg gtcgaggacg atgaacgcct cgccaaactg accgccgagt acctgcacaa
     1141 gaacgatttc gaggtccttg tcgaggcgaa tggcaacaag gccgagacgc gcatcctgga
     1201 cgagcgaccg gacctggtga ttctcgacat catgctgccc ggcaaggacg ggtttgaaat
     1261 ctgccgggcc gtccgcgccc aatatcgcgg ggtgatcctg atgctcaccg cacgtgacga
     1321 agacttcgac cagattctgg gcctcgaact gggcgccgac gactacatca ccaagccggt
     1381 gcagccccgc ctgctgctgg cgcgcatcaa ggccctgctg cgccgcgcgc cgggcaaccc
     1441 cgtcgttgcc gatgaaagcg ctgaaccgag cgagccggct gaactgtcct tcggcaggtt
     1501 ccgcatcagc caggcgacgc gcagcacgca acttggcgac gacggcatcg acctcacgac
     1561 ggcagagttc gacctgctgt ggctgcttgc ccgccacgcc ggcagcatcc tgtcgcgcga
     1621 cgatctgctc caggaactgc gtggcatcgg tttcgacggc ctcgaccgat cgatcgacgc
     1681 gcgcatctcg aggctgcgcc gcaagctcgg tgacgacccg gaaaacccca cgcgcatcaa
     1741 gaccgtgcgc ggcaagggtt atctgttcag caagcatgac tggcaataac aagctcctgg
     1801 cgatcctcgg gaattacaag cccgcgccct ggcgcgggcg ctacagcctg tcgcggctgt
     1861 tcttcaactt ctacctgctg gtcatgggtt ccttcgtggc catcgcaatt tttgccgact
     1921 tcgtcatctc gaccgcagtc aaggggatca ccgacgatta caccagtcgt ttcatgcgcg
     1981 gcaccgtcct gctgatcgag gaggagcttt tccggaagcc gcgttcggag tggccgagga
     2041 cgatcgaggc acttgatcgc aaattcgcct acaaactcga aatcgtcgac cgctggacct
     2101 tgaatctccc ggcggcacag gccgagaagc tcgacggagg ggaactggcg atcgacgcgc
     2161 agggagacat catctaccac cgcctcaagc agacgccaca gattctcgtc gtcggaccga
     2221 tctctcccga acgcaacccc gaataccggc gcggcatgcc gctcgacctg cgcatcagcc
     2281 tgctgacctg gagcctgatc ggtgtatgcc tggcgatcgt ggtctggctg tgggtgcatc
     2341 cggtctggcg agacctcgaa gcgatgcgtc agaccgcgcg cgccctcggc gaaggcctct
     2401 tcgaggcacg cgcgccaatc atgcgcagca gcgccttcgg accgctggcc gaaaccctga
     2461 acggcatggc cgagcgcata cagcggctga tcgccacgca gaaggagttg tcgagcgcca
     2521 tctcgcacga gttgcgtacg cccattgcgc gcctgcgctt cgcgctcgag atgctcgccg
     2581 aaacccatga cgccgccgaa ggcgagcgtt tgtcgcgcat gatggagtcc gacctcgatg
     2641 aactcgacgg
//
LOCUS       DAOSIT010000326         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33657_length_2644_cov_11.334106, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000326 DAOSIT010000000
VERSION     DAOSIT010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33657_length_2644_cov_11.334106"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLL14_08210"
     CDS             complement(<1..188)
                     /locus_tag="PLL14_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG03952.1"
                     /translation="MKPVWIVDDDKSIRWVLEKTLSREKIPFQSFSSATEALARLDAG
                     GEAPQILVSDIRMPGQSGL"
     gene            complement(249..1355)
                     /gene="glnL"
                     /locus_tag="PLL14_08215"
     CDS             complement(249..1355)
                     /gene="glnL"
                     /locus_tag="PLL14_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HOG03953.1"
                     /translation="MSGGHGGNDKRIPESSGNPFGGLDLLSSAVVLLDERLAIRYLNP
                     AAENLLAISNKVFAGCPLGGVIECPPKLLAALHRVLDHGWGYTGQSIELRLAGGDVLQ
                     LNCTVNPVDAPEARLLVELWLIDQQLRATREERLVEQQHANRELIRNLVHEIKNPLGG
                     IRGAAQLLEHELNNPGLREYTQVIIKEADRLQDLMRRLLSPHRPMQPGPVNIHEILER
                     VRSLLTAEFPTTLSVRRDYDTSLPELVGDREQLIQVFLNIARNAAQAIRRQPDGSVSA
                     QGQITLRTRAARQVTLFKRRHRLALEVQVLDDGPGIPDDIRERMFYPLVSGRAGGSGL
                     GLTLAQSFVQQHQGTIDCESRPGHTCFTIRLPLA"
     gene            complement(1500..1985)
                     /locus_tag="PLL14_08220"
     CDS             complement(1500..1985)
                     /locus_tag="PLL14_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOG03954.1"
                     /translation="MKLQDLCLSIALPLLVTVPVHAQDIYKCADGDGRITYSNVPTKS
                     CRKLVLDPVNLAPAAKPPAARVATPPGFPKVDDQTQKSRDGDRRRILEGELAAEQKNA
                     EQAKKDLAEQEAIVLPSERMQGGAISGGKVQERIQQYRDKVALHQRNIEAIQKEIGNL
                     R"
     gene            complement(2115..>2644)
                     /gene="glnA"
                     /locus_tag="PLL14_08225"
     CDS             complement(2115..>2644)
                     /gene="glnA"
                     /locus_tag="PLL14_08225"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807055.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOG03955.1"
                     /translation="ALFYIGGIIKHAKALNAITNPGTNSYKRLVPHYEAPVKLAYSAK
                     NRSASIRVPHVASDKARRIETRFPDPIANPYLCFSALLMAGLDGIQNRIHPGDPADKN
                     LYDLPPEEDAKIPTVCASLEEALASLDKDREFLTRGGVFSDDWIDAYLELKMDEVNKV
                     RMTTHPVEFELYYSC"
BASE COUNT          480 a          801 c          823 g          540 t
ORIGIN      
        1 aggcccgact ggcccggcat gcggatgtcg gaaaccagga tttgtggcgc ctcaccaccg
       61 gcgtcgaggc gcgccagtgc ttcggtcgcc gaagaaaaac tctggaacgg gattttttcg
      121 cgggaaaggg tcttctcgag gacccaacgg attgatttat cgtcgtcgac gatccagacc
      181 ggtttcattg tttcctcagg cggattggca agtatcatga ttcgtaagca aaaaacacgc
      241 caaccgtctc aggccagggg caagcgaatc gtgaagcagg tgtggcccgg tcgactttca
      301 caatcaatcg tgccctgatg ctgttgcacg aaactttgcg ccagtgtcag ccccaggccg
      361 ctgccacctg cccgacccga caccagggga taaaacatgc gttcgcgaat gtcgtcggga
      421 attcccggcc catcgtccag tacctgcact tcgagggcca gacggtggcg tcgcttgaac
      481 agggttacct gccgggcggc acgcgtgcgc agggtgatct gtccctgcgc ggagacgctg
      541 ccgtctggct gccgccggat ggcctgcgct gcattcctgg caatattcag gaaaacctgg
      601 atcaattgtt cccggtcgcc aaccagttcc ggcagactgg tatcgtagtc acgccggacg
      661 ctgagcgtgg tcggaaactc ggcggtcagc aggctgcgca cccgttccag gatctcgtgg
      721 atgttcaccg ggccgggctg catcgggcgg tgcggcgaca gcaggcgccg catcaaatcc
      781 tgcaggcggt cggcctcctt gatgatgacc tgggtgtatt cgcgcaaacc gggattgttg
      841 agttcgtgtt caagcaactg cgccgcaccg cggatgccac ccagaggatt cttgatctca
      901 tgcaccaggt tacgtatcaa ctcgcgattg gcgtgttgct gttcgaccag gcgctcttcg
      961 cgtgtggccc ggagttgttg atcgatcagc caaagctcga ccagcaggcg agcctccggg
     1021 gcgtcgacgg ggttgaccgt gcagttcagt tgcagaacgt cgccacccgc gagtcgtagc
     1081 tcgatacttt gcccggtata gccccaacca tggtcaagga ctctgtggag cgcggccagc
     1141 agtttcggcg ggcactcgat gacgccgccc aacggacagc cggcgaagac cttgttgctg
     1201 atcgccaaca ggttttcggc ggcaggattc aggtagcgga tggccaggcg ctcgtcgagc
     1261 agaaccaccg ccgacgacag caggtcaagc cctccgaacg gattccccga actctcggga
     1321 attcgcttgt cattaccgcc gtgcccgccg ctcaccactg cgtcctttcc gcctcgcctt
     1381 tgacccggaa gatccgccgg gcggccaggg cgcggtcccc ctgtgtctgt cgatccattc
     1441 tgggggtgcc ggcagaatca ggcgctaccg acggaggcca caccggcagt cctcgacgct
     1501 cagcgcagat tgccgatctc tttctggatg gcttcgatgt tgcgctggtg caaggccacc
     1561 ttgtcgcggt attgctggat acgctcctgc accttgccgc cgctgatcgc accaccctgc
     1621 atacgttcgc ttggcaggac gatcgcttcc tgttcggcga ggtccttctt cgcctgctcg
     1681 gcatttttct gctcggcggc caattcgccc tcgagaatgc gtcggcggtc gccatcgcgc
     1741 gacttctgcg tctgatcgtc cactttcggg aagccgggag gcgtcgccac gcgagccgcc
     1801 ggcggtttcg cagccggcgc gagattgacc gggtcgagta caagcttcct gcagctcttg
     1861 gtcggcacat tggagtaggt gatacgcccg tcaccgtcgg cacacttgta gatgtcctgc
     1921 gcgtgcacgg gtacggtgac cagcaacggg agtgcgatcg aaagacacag gtcttgcagt
     1981 ttcatcgtat tcgtattcct tggtcgcaac caactcgccg gtggagacgg gcaaagcaat
     2041 actaacgcgg cgggaagacg atcggtagac aaaaaaagga cgggcaagcc cgtcctcctc
     2101 tctgcgcagc ctgatcagca gctgtagtac agttcgaatt ccaccgggtg ggtggtcatg
     2161 cgcaccttgt tgacctcgtc catcttcaat tcaagatagg cgtcgatcca gtcgtcggaa
     2221 aagacgccgc cgcgggtcag gaactcgcgg tccttgtcca gcgaggcgag tgcttcttcg
     2281 aggctggcac acacggtcgg gatcttcgca tcctcttccg gcggcagatc gtagaggttc
     2341 ttgtctgccg gatcgccagg atgaatccgg ttttgaatgc catcgaggcc ggccatcagc
     2401 agtgccgaga agcacaggta gggattggcg atcgggtccg ggaagcgcgt ttcgatgcgc
     2461 cgcgccttgt ctgaagccac atgcggcacg cggatcgatg ccgagcggtt cttggccgaa
     2521 taagcgagct tgaccggcgc ctcgtagtgc gggaccagcc gcttgtacga attcgtaccg
     2581 ggattggtga tcgcgttcag cgccttggcg tgcttgatga tgccgccgat atagaagagc
     2641 gcca
//
LOCUS       DAOSIT010000327         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33748_length_2639_cov_12.983359, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000327 DAOSIT010000000
VERSION     DAOSIT010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33748_length_2639_cov_12.983359"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1464)
                     /gene="rng"
                     /locus_tag="PLL14_08230"
     CDS             complement(10..1464)
                     /gene="rng"
                     /locus_tag="PLL14_08230"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HOG03956.1"
                     /translation="MPEEILINFTPQETRVAVTHQGVVQELHIERSASRGFVGNVYIG
                     RIVRILPGMQSAFIDIGLGRTAFLHVADIREPRPSDEPVRPIERLLFEGQSILVQVIK
                     DPMGSKGARLSTQVSIAGRMLVYLPQDKHIGISQRIERESDREALREKLGRLVPGEET
                     GGFIVRTMAESATEPELAKDIEYLRKLWRDIQTQATTAAPPSLLYQELSLAQRVLRDF
                     VNPETARIVIDSRENFQRLTAFAAEYTPTVAPLLEHYIGERPLFDLHGVEDELQKALA
                     RRVDLKSGGYLIIDQTEAMTTIDVNTGGFVGVRNFDDTIFKTNLEAAQTIARQLRLRN
                     LGGIIIIDFIDMENEEHRSAVLSEFNKALARDHTRLTVNGFTALGLVEMTRKRTRESL
                     AHVLCEECPTCGGRGEVKTARTVCYEILRELLREARQFNAREYRVLGNQAVIDRFLDE
                     ESQGLAMLSDFIGKPISLQAEPSYSQEQYDIVLM"
     gene            1594..>2639
                     /gene="yegQ"
                     /locus_tag="PLL14_08235"
     CDS             1594..>2639
                     /gene="yegQ"
                     /locus_tag="PLL14_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HOG03957.1"
                     /translation="MLRTALAFGADAVYAGQPRYSLRVRNNDFGDLELLARAVDEAHA
                     AGRKLYVVSNVLPHNRKVRSYLNDMAPVVALQPDALIMADPGLIDLVRETWPAMPVHL
                     SVQASTVNFAAVRFWRKLGLTRIILSRELSLDEVAEIRQECPDIELEVFVHGALCVAY
                     SGRCLLSGYFNHRDPNQGTCTNSCRWEYQLRAAEADASGDVRAAAPPPGQPGDVWLLE
                     EKERAGELMPIDEDEHGTYIMNSRDLRAVEHVRRLTEIGVDSLKIEGRTKSPYYVART
                     AQVYRRAIDDAVARRPFDPGLLGELEGLANRGYTDGFYQRHHEHEMQNYLRGHSESGR
                     SLYVGDVLQWDAPRR"
BASE COUNT          441 a          839 c          857 g          502 t
ORIGIN      
        1 ggtcaccgac tacatcagca cgatgtcgta ctgctcctgc gagtagctgg gctcggcctg
       61 cagcgagatc ggtttgccga tgaaatcgga gagcatcgcc agtccttgcg actcctcgtc
      121 gagaaagcga tcgatcaccg cctggttgcc gagcacgcgg tactcgcgag cgttgaactg
      181 acgcgcctcg cgcaggagtt cgcgcaggat ctcgtagcat accgtgcgcg ccgtcttgac
      241 ttcgccgcgg ccaccacagg tcggacattc ctcgcacagc acgtgcgcga gcgactcgcg
      301 cgtccgcttg cgggtcatct cgaccagacc cagcgcggtg aagccgttga cggtcaggcg
      361 cgtgtggtcg cgcgccagcg ccttgttgaa ttcgctgagg acggcgctgc gatgctcttc
      421 gttctccatg tcgatgaagt cgatgatgat gatgccgccg aggttgcgca ggcgcagttg
      481 ccgggcgatc gtctgcgcgg cttcgaggtt ggtcttgaag atcgtgtcgt cgaaattgcg
      541 taccccgaca aagccaccgg tattgacgtc gatggtggtc atcgcctcgg tctggtcgat
      601 gatcaggtaa cccccggact tgagatcgac gcgccgggcg agcgccttct gcagttcgtc
      661 ttccacccca tgcaggtcga aaagcgggcg ttcgccgatg tagtgctcaa gcagaggcgc
      721 gactgtcggc gtgtattcgg cggcgaaggc cgtcagccgc tgaaagttct cgcgcgagtc
      781 gatgacgatg cgggcggtct cgggattcac gaaatcgcgc agcacacgct gtgccagcga
      841 cagttcctgg tacagcaggg acggtggtgc agcggtggtc gcctgcgtct ggatgtcgcg
      901 ccagagtttg cgcaggtact cgatgtcctt ggccaactcg ggttcggtgg cactctcggc
      961 catcgttcgc acgatgaatc cgccggtttc ttcaccgggc accagccgac cgagtttttc
     1021 gcgcagcgct tcgcgatcgg actccctctc gatgcgctgc gagatcccga tgtgcttgtc
     1081 ctgcggcagg tagaccagca tccgtccggc gatcgaaacc tgcgtggaca ggcgcgcgcc
     1141 cttgctgccc atcgggtcct tgatcacctg cacgaggatg ctctgtccct cgaacagcag
     1201 gcgctcgatc ggtcgcaccg gctcgtcgct ggggcgcggt tcgcggatgt cggcaacatg
     1261 caggaacgcg gtacggccca ggccgatgtc gatgaacgcc gattgcatgc ccggcaggat
     1321 gcgcacgatg cgtccgatgt agacgttgcc gacgaagccg cgactggcgc tgcgttcgat
     1381 atgcagttcc tgcacgacgc cctgatgcgt gacggcgacg cgcgtttcct gcggagtgaa
     1441 gttgatcagg atctcttcgg gcatggcggg gcgatctgga gaatgagagt agaatttcgc
     1501 tttgctgcat tctaccccgt cacgcccctt ccccggactt ctgccgcctt gaccagtgcc
     1561 cccgagttgc tcgcccccgc cggttcgctg gcgatgttgc gcacggctct ggcgtttggt
     1621 gccgacgcgg tgtatgccgg ccagccgcgc tacagcctgc gcgtgcgcaa caacgacttc
     1681 ggcgatctcg aactgctggc gcgcgcggtc gacgaggcgc atgccgccgg ccgcaagctc
     1741 tacgtggtca gcaacgtcct gccgcacaac cgcaaggtcc gcagctacct gaacgacatg
     1801 gcgccggtgg tcgcgctgca gcctgacgcg ctgatcatgg ccgaccccgg gctgatcgac
     1861 ctggttcgcg aaacctggcc ggcgatgccc gtccatctgt cggtgcaggc cagcacggtg
     1921 aactttgccg cggtgcgttt ctggcgcaag ctgggcctga cccggatcat cctgtcgcgc
     1981 gaactgtcgc tggacgaagt tgccgagata cgccaggaat gcccggacat cgaactggaa
     2041 gtctttgtgc acggcgcgct gtgcgtcgcc tattcgggac gttgcctgct ctccggatac
     2101 ttcaaccatc gtgacccgaa tcagggcacc tgtaccaact cgtgtcgttg ggaataccag
     2161 ctgagggcgg cagaggcgga tgcgagcggc gacgtccgtg ccgccgcgcc gccaccgggc
     2221 caacccggcg acgtgtggct actcgaggaa aaggagcgtg ccggtgaact gatgcccatc
     2281 gacgaagacg agcatggcac ctatatcatg aattccaggg atctgcgcgc cgtcgagcac
     2341 gtccggcgcc tgaccgagat cggcgtcgac tcgctgaaga tcgaggggcg caccaagtcg
     2401 ccctactacg ttgcgcgcac cgcgcaggtc tatcggcgcg ccatcgacga cgcggttgcc
     2461 aggcgtccgt tcgaccccgg cctgctcgga gaactcgagg gactggccaa tcgcggctat
     2521 accgacggtt tctaccagcg tcaccatgaa catgaaatgc agaactacct gcgtggccat
     2581 tccgaatcgg ggcgcagcct ctacgtcggc gacgtgctgc aatgggatgc gccgcgccg
//
LOCUS       DAOSIT010000328         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_33874_length_2632_cov_8.459061, whole genome shotgun sequence.
ACCESSION   DAOSIT010000328 DAOSIT010000000
VERSION     DAOSIT010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33874_length_2632_cov_8.459061"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLL14_08240"
     CDS             <1..512
                     /locus_tag="PLL14_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOG03958.1"
                     /translation="GYEDGTENPTGDAAASTAIIGDERPGLTGSSFVAVQRWTHDFAR
                     FEAMPGTQQDLSFGRRRADNQEIDDAPASAHVKRTAQESFAPAAFVLRRSMPWAEGNR
                     GGLLFVAFATSFDPFEAQLRRMLGIEDGIVDALFKFTRPQSGAYFWCPPMRKGRLDLS
                     ALGLSTAAW"
     gene            682..2331
                     /locus_tag="PLL14_08245"
     CDS             682..2331
                     /locus_tag="PLL14_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG03959.1"
                     /translation="MTFSCIFRKVPAALSLLLLACGFLLASAAIPALAAEQAHDKEAT
                     LSYHFRPIVTFRATFVGATPLIRVRRALTRIDALPPNQMEQPIELTPFTADGTHGFIL
                     RIGEFVLFSILDGDLDPEEKISLEAAARRTAGEMSVALQAAATQRHPMVLLKGVGLAA
                     AATLIALILLWLIRRATTLVVGHLQHVIEAEDASNSLRWARHGWLLVQRVAQLLMGFL
                     WLSVAYLWLTYVLARFPLTEPLGDRLSDFLFGLLYTLGVGLVNAMPALTTALIILFLT
                     KSFNDAIGNFFKAAREGRVQVPGLHPETVTATHRIVSILIWGLGIAVAYPFIPMSDSD
                     AFKGLSVMFGFMLTLGSAGIVNQLMSGLVLVYSRALSVGDFVDIGENVGVVSEVGALS
                     TKMINMRNEEVTIPNAVLVGSPIRNYSRLAGARGTLVSTKVTIGYDTPWRQVHAMLIA
                     AAEQTPGLRASPRPFVYQRSLADWYVEYELFAHMDKPLERVPVLSALHAHIQDQFNTY
                     GVQIMSPHFVVQPSRNVVVPPEHWHAAPAAPEGSPGQTEHH"
BASE COUNT          440 a          882 c          804 g          506 t
ORIGIN      
        1 ccggctacga ggacggcacc gagaacccga ccggcgacgc cgcggcaagc accgcgatca
       61 ttggcgacga acgtccgggg ctcacgggtt cgagtttcgt cgccgtgcaa cgctggacgc
      121 acgattttgc tcgtttcgaa gccatgcccg gcacgcagca ggacctcagc ttcggtcgcc
      181 gacgcgccga caaccaggaa atcgacgatg ccccggcgtc ggcacacgtc aaacggacgg
      241 cgcaggaaag ctttgccccg gcggctttcg tgctgcgccg gtcgatgcca tgggccgagg
      301 gcaaccgcgg tgggctgctg ttcgtggcct tcgccacctc cttcgatcct ttcgaggcac
      361 agttgcgccg gatgctgggc attgaagacg gcatcgtcga tgcgctgttc aagttcactc
      421 gcccacagtc cggcgcctat ttctggtgcc caccaatgcg caagggacgg ctcgacctct
      481 cggcactggg cttgtcaacc gccgcgtggt gagcaatccc tgcgtcggca ggactctcca
      541 ggcctgccga cgcccgggag gtgatgctgc gctgccgacc gaatccatgt tccgcgcctg
      601 gctggccgct tcggggcacg tcgacgagcg atttcggcag ctataatccg gcactgactc
      661 tacaaccagc cgaatttcct catgaccttc tcctgcatct tccggaaagt acccgcagca
      721 ctgtcgctgt tgttgcttgc ctgtggtttc ttgctggcca gcgctgccat tccggccctc
      781 gcggcggagc aggctcacga caaggaggcc actctcagct accacttccg tccgattgtc
      841 accttccggg cgaccttcgt cggcgcaacg ccgttaattc gtgtacggcg ggcgctgacc
      901 cggatcgatg ccctgccgcc aaaccagatg gagcagccga tcgagctgac gccattcact
      961 gccgatggca cgcacggctt catcctgcgt atcggggagt tcgtcctgtt cagcatcctg
     1021 gacggcgacc tcgacccgga ggagaagatt tcgctcgaag cggcagccag gcgtactgcc
     1081 ggtgaaatgt cagtggccct gcaggcggca gcgacgcagc gccatccgat ggtgctcctg
     1141 aagggtgtcg ggctggcggc ggcggccacg ctgatcgcac tgatcctgct gtggctgatc
     1201 cggcgcgcga ccacgctggt ggtcggccac ctgcagcacg tgatcgaagc cgaggacgcc
     1261 tccaacagtc tgcgctgggc gcggcacggc tggttgctgg tacagcgagt cgcccaactg
     1321 ctgatgggtt tcctgtggtt gagcgtcgct tacctctggt tgacctacgt cctggcacgc
     1381 tttccgctca ccgaacccct cggcgaccgg ctcagcgatt tccttttcgg gctgttgtac
     1441 accctcggcg tcggcttggt caatgccatg ccggcgctga cgacggcgct gatcatcctg
     1501 ttcctgacca agtccttcaa cgatgcgatc ggcaattttt tcaaggccgc gcgagaaggc
     1561 cgggtacagg tgccgggcct gcacccggaa acggtcaccg ccacccaccg gatcgtcagc
     1621 atcctgatct ggggtctggg aatcgctgtc gcctatccgt tcattccgat gtccgacagt
     1681 gacgccttca agggcttgtc ggtcatgttc ggcttcatgc tcacgctcgg ctcggccggg
     1741 atcgtcaacc agttgatgag cggcctggtc ctggtctatt cgcgcgcgct ctcggtgggt
     1801 gacttcgtcg atatcggcga aaacgtcggg gtcgtcagcg aggtcggcgc gctgtcgacc
     1861 aagatgatca acatgcgcaa cgaggaagtg acgattccca acgcggttct ggtcggcagt
     1921 ccgatcagga actactcccg gctagccggt gcgcgcggca cgctggtgtc gaccaaggtg
     1981 accatcggtt acgatacacc gtggcggcag gtgcatgcca tgctgatcgc tgctgcagaa
     2041 cagacgccgg gtttgcgggc ttcgcccagg cctttcgtct accaacgcag cctggccgac
     2101 tggtacgtcg agtacgagtt gttcgcgcac atggacaaac cgctcgaacg cgtgccggtg
     2161 ctgtcggcac tgcatgccca catccaggac cagttcaaca cctacggagt acagatcatg
     2221 tcgccgcatt ttgtcgtcca gcccagccgc aacgtggtcg tcccgcccga gcactggcac
     2281 gcggcaccgg ctgccccgga gggctcccca ggtcagactg aacaccactg actccctggc
     2341 caagtcgata tccgtttcag tgctgcgaag tcggcttgcc gacggcgaac cctggcctgc
     2401 cgccacgccg agcctgaccg ggatggcggt acaagtttcc cgggatgacg aaagccatcc
     2461 ctcccccgcc cactctccat cccgctctcc cgccagcgtg cgggaagggg ttttaagtcc
     2521 ctggcgcgaa gatcaccctg agccaggagc gcggatcccg gccagggggc cgcaccccga
     2581 caacatttcg cgtttcttcg aaagtcccga tgatcagctt cgccaccacc gt
//
LOCUS       DAOSIT010000329         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_34036_length_2622_cov_8.830931, whole genome shotgun sequence.
ACCESSION   DAOSIT010000329 DAOSIT010000000
VERSION     DAOSIT010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34036_length_2622_cov_8.830931"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1282
                     /locus_tag="PLL14_08250"
     CDS             94..>1282
                     /locus_tag="PLL14_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOG03960.1"
                     /translation="MSNFIVRKAAVLGAGVMGAQIAAHLANAEVPVVLFDLPAREGDP
                     NGIVNKAIDALRKLEPSPLATKDRVSYIDAANYSQHLDQLKDCDLIIEAIAEKMEWKD
                     DLYRQISPFVAPSAIIASNTSGLSINRLSEALPAALRSRFCGIHFFNPPRYMHLVELI
                     ATRSTDPALLDNLESWLVSRLGKGIVRALDTPNFVANRVGVFSMLAVMHHTQRLGLGF
                     DVVDALTGPIIGRPKSATYRTADVVGLDTLAHVVKTMQDTLPEDPWHAYYSVPDWLAA
                     LIGKGALGQKTRCGIFRKDGRAIKVLDLASQDYRESAGDIDPTVLAILKNRNPAEKFA
                     QLRASEHPQAQFLWAIFRDIFHYAAFHLADIADNARDLDFAMRWGFGWSQGPFESWQA
                     AGWR"
     assembly_gap    1283..1382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1383..2202
                     /locus_tag="PLL14_08255"
     CDS             <1383..2202
                     /locus_tag="PLL14_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOG03961.1"
                     /translation="GLVLWHEAPFAFGANLKEVAEAIVGGEFELLEQYVGEFQNASMC
                     LKYAQVPVVAAVQGMALGGGCEFLMHAARRVIALESYIGLVEAGVGLIPAGGGCKEFA
                     IRAAQHAAKTGGNDPFEFIQPVFMTIAMATVSKSGAQAKELGFAVESDKIVFNANELL
                     YVALREARGLAEAGYAPRLSPRAIKVAGRTGIANCEMMLANMKEGGMISAHDYVVAKA
                     AATALCGGDIETGSVVDEQWLLSVERKLFVELLKNPKTQQRIQHMLETGKPLRN"
     gene            2223..>2622
                     /locus_tag="PLL14_08260"
     CDS             2223..>2622
                     /locus_tag="PLL14_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOG03962.1"
                     /translation="MSKQIQDAYIVAATRLPVGRRKGMLGHVRPDDMLAHAIRSVLAQ
                     APGLDPAMVEDVIVGCAMPEAEQGMNVARIGLLLAGLPNSVPGVTVNRFCASGLQTVA
                     MAADRIRLGEAEVMIAAGTETMSLMNQMMGN"
BASE COUNT          477 a          773 c          808 g          464 t
ORIGIN      
        1 gcgtataatt caaacgttcg ttttagcggc tttgtgcaga gtcgctacgg ggtgttcccg
       61 acgagcgtag acgactcaga caaggaggac agcttgagca acttcatcgt acgcaaggcc
      121 gccgtactcg gagcgggtgt gatgggcgcg cagatcgccg cgcacctcgc gaatgccgaa
      181 gtgccggtgg tgcttttcga cctgccggcc agggagggtg atcccaacgg gatcgtcaac
      241 aaggccatcg acgccctcag gaagcttgag ccgtcgccac tggccaccaa ggatcgcgtc
      301 agctacatcg acgccgccaa ctattcgcaa cacctcgatc aactcaagga ttgcgacctg
      361 atcatcgaag cgattgccga aaaaatggag tggaaggacg atctctatcg tcagatttcg
      421 ccctttgtcg cgccgagcgc gatcattgct tcgaatacct cgggcctgtc gatcaaccgc
      481 ctctcggagg cgctgccggc agcgctgcgc tcgcgattct gcggcatcca cttcttcaat
      541 ccgccgcgct acatgcacct cgtcgaactg atcgccacca ggagcaccga tccggcgttg
      601 ctcgacaacc tcgagtcctg gctggtctcg cgcctcggca agggcatcgt ccgcgccctc
      661 gatacaccga atttcgttgc caatcgcgtt ggcgtgtttt cgatgctggc ggtgatgcac
      721 catacccagc ggctcgggct gggcttcgac gtcgtcgatg ccttgaccgg accgatcatc
      781 ggtcgcccga agagcgccac ttatcgtacc gccgacgtgg tcggtctgga tactctggcg
      841 catgtcgtca agaccatgca ggacaccctg cccgaagatc cctggcatgc ctattacagc
      901 gtgcccgatt ggctggccgc gttgatcggc aagggcgcgc tcggccagaa gacgcgctgc
      961 ggcatcttca gaaaggacgg gcgggcgatc aaggtgctcg acctggccag ccaggactat
     1021 cgcgagagtg ccggcgacat tgatccgacg gtgctggcga tcctcaagaa tcgcaatcct
     1081 gccgaaaaat ttgcgcaatt gcgcgccagc gagcacccgc aggcgcagtt cctgtgggcg
     1141 atcttccgcg atatcttcca ctacgccgcc ttccatcttg ccgacattgc cgacaatgcc
     1201 cgcgacctcg atttcgcgat gcgctggggt ttcggctggt cgcaagggcc attcgaaagc
     1261 tggcaggccg ccggttggcg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nngggcctgg tgctgtggca cgaggcgccc ttcgcatttg gcgccaatct caaggaggtg
     1441 gccgaagcga tcgtcggcgg cgaattcgaa ctgctggaac aatatgtcgg cgaattccag
     1501 aacgcttcga tgtgcctgaa gtatgcccag gtgccggtgg tcgcggcggt gcagggcatg
     1561 gccctgggcg gcggctgcga atttctgatg cacgccgcca ggcgggtgat tgcgctggag
     1621 agctacatcg gcctggtcga agccggcgtc ggtctgattc cggccggtgg cggatgcaag
     1681 gagtttgcca tccgcgccgc gcaacacgcc gcgaagaccg gcggcaacga tccgttcgag
     1741 ttcattcagc cggtgttcat gaccatcgcc atggccacgg tgtcgaagag cggtgcgcag
     1801 gcgaaggaac tgggttttgc cgtcgaatcc gacaagatcg tgttcaatgc caacgaactg
     1861 ctatatgtgg cgctgcgtga ggcgcgcggt ctcgccgagg cgggctatgc cccacgcctg
     1921 tcgccgcgcg cgatcaaggt cgccggccgc accggcatcg ccaactgcga aatgatgctg
     1981 gccaacatga aggaaggcgg catgatttcg gcgcacgact atgtcgtggc caaggctgcc
     2041 gcgacggccc tgtgtggtgg agacatcgag accggatccg tggtcgacga gcagtggttg
     2101 ctgagcgtcg agcgcaagct cttcgtcgag ctgctgaaga acccgaaaac ccagcagcgt
     2161 atccagcaca tgctggaaac cggcaagccg ctgcgtaact gagcgagcaa ggagaacaga
     2221 tcatgagcaa acagattcag gacgcgtaca tcgtcgccgc cacgcgtctg ccggtcggcc
     2281 gtcgcaaggg catgttgggt cacgttcgtc cggacgacat gctggcgcac gccatcaggt
     2341 cggtactggc ccaggcgccg ggccttgacc cggcaatggt cgaggacgtg atcgtcggct
     2401 gcgccatgcc ggaagccgag cagggcatga atgtcgcgcg catcggcctg ctgctggcag
     2461 gcctgccgaa cagcgtaccc ggagtgaccg tcaaccgctt ctgcgcctcc ggtttgcaga
     2521 ccgtggcgat ggccgccgat cgcattcgcc tcggcgaagc cgaggtgatg atcgctgccg
     2581 gtaccgaaac gatgtcgctg atgaaccaga tgatgggcaa ca
//
LOCUS       DAOSIT010000330         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_34125_length_2617_cov_10.779859, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000330 DAOSIT010000000
VERSION     DAOSIT010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34125_length_2617_cov_10.779859"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1883)
                     /locus_tag="PLL14_08265"
     CDS             complement(<1..1883)
                     /locus_tag="PLL14_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03963.1"
                     /translation="MRRLSLMNRLSLTAVLALLAMLLIVWESLWSLHGLLYADRQVKT
                     RHLVETVTSVVDHYYGLQKAGTLTEEEARQQAIAAVKRLRYEKAEYFWINDLGKPVPK
                     MVMHPTVPALDGKVLDEARFNKAIFLQAGLNGDKLPADGKNLFVSFNEVVEKAGEGYV
                     EYLWPKPLAGGGVTSELFTKLSYVKKFEPWGWVIGSGIYIDDVDRIFREEAMLSVLLA
                     LAATVVLLLSGWVVRKSIVSEFGGEPRLALGVTSRIAEGDLTHEIPLRAGDQGSVLSV
                     LAHMQDSLKAMLRAVFTNANRLQASVERLSAQSNEINLATQVQASAVSHTRSAINDVS
                     ASVEVVNGLVHATEDGAHEVARRAHDGAAVAAKVAGEMQAIADTVAVSSDQVSRLVAS
                     TGEIGQMAQVIKEIADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTSKATS
                     EIGGILQGIRSDTESAVEGMRAASPVIASGVTQANSAAETLHAIEEGAQDTLEKMQAL
                     SQATRDQTRRIEEIVSNVDEVMSASGQTENVIKQSLQSAADLEAASSEMFSMVQRFKI
                     GELGDRWPTAAGGQAATVKPLMEWSSALAVGHAEIDRQHQVLIDIANRLNAAMQSGAG
                     RAA"
     gene            complement(2106..>2617)
                     /locus_tag="PLL14_08270"
     CDS             complement(2106..>2617)
                     /locus_tag="PLL14_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG03964.1"
                     /translation="IINREWGLAMNENPNQGSFIIEELTDLVEEAVLKEFEAIAARGG
                     VLGAMETGYQRGKIQEESLYYEHKKHDGSFPIVGVNTFRNPQGDAQIEIELARSTEEE
                     KQSQLTRLRDFQGRNAAASGPMLEHLKHTVIENGNVFAVLMDAVRVCSLGQITTALYE
                     VGGQYRRSM"
BASE COUNT          458 a          868 c          754 g          537 t
ORIGIN      
        1 caggccgcac gaccggctcc cgactgcatg gcggcgttca ggcggttggc gatatcgatc
       61 aggacctggt gctggcggtc gatttcggca tgcccgacgg ccagcgcact cgaccactcc
      121 atcagcggct tgactgttgc ggcctgacca cccgccgctg tcggccatct gtcgcccaac
      181 tcgccgatct tgaagcgctg caccatcgag aacatctcgc tggacgccgc ttcaagatcg
      241 gctgccgatt gcagtgactg cttgatgacg ttttcggtct gtccggaagc gctcatcacc
      301 tcgtctacat tgctgacgat ctcttcgatc cgccgcgtct ggtcacgggt cgcctgcgac
      361 agcgcctgca ttttttccag cgtgtcctgc gccccctcct cgatggcatg cagggtttcg
      421 gcagcggagt tggcctgggt cacgccgctg gcaatcaccg gcgaggccgc gcgcatgcct
      481 tccaccgcac tctccgtatc gctgcggatg ccctgcagga tgccgccgat ttcgctggtg
      541 gccttgctcg ttcgctcggc cagtttgcgc acttcgtcgg cgaccaccgc gaagccgcgt
      601 ccctgctcgc cggcgcgtgc ggcttcgatg gccgcattaa gggcaagcag attggtctgg
      661 tcggcaatct ccttgatcac ctgggccatc tgcccgatct cgccggtact cgccaccagc
      721 cgggaaacct ggtcagacga aacggcaacc gtgtcggcga tggcctgcat ttcaccggca
      781 actttcgccg ccaccgcggc accgtcatgg gcacgtcgcg ccacttcgtg cgcgccgtcc
      841 tcggtggcat gcacaagtcc gttcacaacc tcgacactcg ccgaaacatc attgatggcc
      901 gatcgagtat gactgaccgc gctggcctgt acctgcgtgg ccagattgat ctcgttggac
      961 tgtgcggaga gccgctcgac gctcgcctgc agtctgttcg cgttggtgaa cacggcccgc
     1021 aacatcgcct tgaggctgtc ctgcatgtgc gccagaaccg acagcacgct cccttgatcg
     1081 cccgcccgta gcgggatctc gtgcgtcagg tcaccttcgg cgatgcggct ggtaaccccc
     1141 agggcgaggc gtggctcacc accgaattcg ctgacgatgc ttttccgcac gacccagccg
     1201 gagagcagca gcacgacggt ggcagcaagc gccagcagaa cgctgagcat ggcttcctcg
     1261 cggaagatcc ggtcgacgtc gtcgatatag atacccgagc cgatgaccca accccagggt
     1321 tcgaacttct tgacgtacga cagcttggta aacagctcgc tggtgacgcc accgcccgcg
     1381 agtggcttcg gccagaggta ctcgacataa ccttcgcctg ctttttcaac cacttcgttg
     1441 aagctgacga agaggttttt cccgtcggcc ggaagcttat cgccattcag gccggcctgc
     1501 aggaagatcg ccttgttgaa acgcgcttca tcgagcacct tgccgtcgag agcgggtacc
     1561 gtcggatgca tgaccatctt cggcaccggc ttgccgaggt cgttgatcca gaagtactcg
     1621 gctttttcgt agcgcaagcg tttcaccgcc gcgatcgcct gctgcctggc ttcctcctcg
     1681 gtcaacgtgc ccgctttttg caggccgtag taatgatcga cgacgctggt caccgtctcg
     1741 accagatgcc gcgtcttgac ctgccgatcg gcgtaaagca gaccgtgcaa cgaccacagg
     1801 ctttcccaga cgatcagcag catggccaga agcgccagta ctgccgtcag cgacaggcgg
     1861 ttcatcagac tcaggcgacg caacgcagca accatctcga cccccccatt gtttcaccag
     1921 cccaactggt aaccagtgat gctgtaccaa tcaagccaaa gatgcaacca tctgtgggtt
     1981 cgaggaggcg caggaacgca gatcactccc cttgggaact gcccagggaa ttcgccgcgt
     2041 tgccctctac gcggcacctc tttcccggac gcagccgcgc gggcgtccgg gaatcgcccg
     2101 gtggactaca tgctgcgtcg atattgaccg cccacttcgt acagcgcggt ggtgatttgc
     2161 ccaagcgaac agacccggac agcatccatg agaacggcga agacattgcc gttttcgatg
     2221 actgtgtgct tgaggtgctc gagcatcgga ccggacgctg ccgcgttgcg accctggaag
     2281 tcgcgcaggc gcgtgagttg cgactgcttt tcctcttccg tcgagcgcgc gagttcaatc
     2341 tcgatctggg cgtcaccctg cggattgcgg aaggtattca ccccgacgat agggaacgaa
     2401 ccgtcatgct tcttgtgctc gtaatagagg gactcctcct ggatcttgcc gcgctgataa
     2461 ccggtttcca tggccccgag tacgcccccg cgcgcggcaa tggcttcgaa ttccttgagc
     2521 acggcctctt cgacgagatc ggtcagttct tcgatgatga acgaaccctg gttcgggttc
     2581 tcgttcatcg ccagccccca ctcgcggttg atgatca
//
LOCUS       DAOSIT010000331         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_34352_length_2605_cov_10.951765, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000331 DAOSIT010000000
VERSION     DAOSIT010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34352_length_2605_cov_10.951765"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1331
                     /locus_tag="PLL14_08275"
     CDS             15..1331
                     /locus_tag="PLL14_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG03965.1"
                     /translation="MSDLFAERPTDAPLAERLRPRSLAEVIGQEHLLGPGKPLAMAFA
                     SGQLHSMILWGPPGVGKTTLARLMADAFAADFIALSAVFSGVKDIREAVARAEMTRAH
                     HGRRTILFVDEVHRFNKAQQDAFLPYVEAGLLTFVGATTENPSFEVNSALLSRASVYV
                     LHPLSAVEMARLFERVCGQILPALSFDDDARARLIGLADGDARRLLNLIEQIGTATAT
                     AQVRSVDGAFIERSLAQSLRRFDKGGDAFHDQISALHKAVRGSSPDAALYWFCRMLDG
                     GADPRYLARRIMRMAWEDIGLADPRAARIALDAAESFERLGSPEGELALADAVLYMAM
                     AAKSNAAYLAYNAARSFVSGDGSRPVPVHLRNAPTRLMKNLGYGANYRYAHDEADAYA
                     AGENYFPEGMPAIEWYRPVARGLEIRIGEKLAQLRELDRQARDKKD"
     gene            1380..>2605
                     /gene="serS"
                     /locus_tag="PLL14_08280"
     CDS             1380..>2605
                     /gene="serS"
                     /locus_tag="PLL14_08280"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG03966.1"
                     /translation="MLDIQLLRNNLDAIADRLATRGFTLDRSTFQRLENERKFLQTRT
                     QELQAGRNSLSRQVGMLKGRGEDAAAVMEQVAAIKNELEANEARLGELLNELDAFLAV
                     LPNLPQDSVPVGRSDVDNVEVKRWGAPRSFAFAVKDHVDLGEALGQLDFATAAKIAGT
                     RFSLMKGQLARLHRVLAQFMLDTHTARHGYTEVYAPYLVNAASLLGTGQLPKFEEDLF
                     KVLRADAEPLYLIPTAEVPVTNIVRDAILTAAELPLKFVCHTPCFRSEAGSYGRDTRG
                     MIRQHQFDKVELVQIVAAGDSLAAHEELTGHAEAILEMLDLPYRRVALCTGDIGFSSA
                     KTYDLEVWLPAQKAYREISSCSNFEAFQARRMQARYRNERNKTELVHTLNGSGLAVGR
                     TLVAVMENFQNTDGSI"
BASE COUNT          480 a          799 c          832 g          494 t
ORIGIN      
        1 tgacgagaac cgggatgtct gacctttttg ccgagaggcc tacggacgca ccacttgccg
       61 agcggctgcg gccgagaagt ttggcggaag tgatcggaca ggagcatctg ctcggtccgg
      121 gaaagccgct ggcgatggca ttcgcctcgg ggcagttgca cagcatgatc ctgtgggggc
      181 cgccgggagt gggcaagaca accctggcac gcctgatggc ggatgccttt gctgccgatt
      241 tcattgccct ctcggcggtc ttctcagggg tcaaggacat ccgtgaagcc gtggcacgcg
      301 ccgaaatgac gcgcgctcac catggccgtc gaacgattct cttcgtcgat gaagtgcatc
      361 gtttcaacaa ggcccagcag gacgcgtttt tgccttatgt cgaggccggg ctgctgactt
      421 ttgtcggtgc gacgacagaa aacccgtctt tcgaggtcaa ttcggcgctg ctctcgcggg
      481 catcggttta cgtgctgcac ccgctctcgg cggtagaaat ggcgcgcctc ttcgaacgag
      541 tttgcgggca gatcctgccg gccttgagct tcgacgacga tgcgcgcgcg cgcctgattg
      601 gccttgccga cggcgacgcc cggcggctgc tcaacctcat cgaacagatc ggtacggcca
      661 ctgcgactgc gcaggtgcgc agtgtcgacg gggctttcat tgagcgatcc ctggcgcaga
      721 gcctgcgtcg cttcgacaag ggcggtgatg ccttccacga ccagatttca gctttgcaca
      781 aggcggttcg cggatcctct ccggatgccg cgttgtactg gttttgccgc atgcttgacg
      841 gtggcgccga tccacgttat ctggcgcgtc gcatcatgcg catggcctgg gaagacatcg
      901 gccttgccga cccgcgggca gcacggatcg cactcgacgc ggcagaatcc ttcgaacgtc
      961 tcggctctcc cgaaggcgaa ctggcacttg ccgatgcggt gttgtacatg gccatggccg
     1021 ccaagtcgaa cgccgcctac ctcgcgtata atgccgcgcg gagcttcgtc agtggtgatg
     1081 ggtcgcggcc cgtgccggtc catctgcgta acgcgccgac gcggctgatg aaaaacctgg
     1141 gttacggggc aaactaccgc tacgcccacg acgaggccga tgcctatgcg gccggcgaga
     1201 actattttcc ggaaggcatg ccggcgatcg agtggtatcg gccggtcgcg cgtggcctcg
     1261 aaatccgcat tggcgagaag ctggcgcagt tgcgtgagct cgatcgccag gcaagggaca
     1321 agaaggacta ggcggcagct tggcggggcg ccgcgaattc gccaaactga ggaccaggca
     1381 tgctcgacat acaactgctg cgcaacaacc ttgacgcgat tgccgacaga ctggcaacgc
     1441 gcggcttcac gcttgaccga tcgacctttc agcggctcga aaacgagcgc aagttcctgc
     1501 agacgcgcac gcaggagttg caggccgggc gcaacagcct ctccagacaa gtcggcatgc
     1561 tcaagggccg cggcgaagac gccgcggccg tcatggagca ggtggcggcg atcaagaacg
     1621 agctcgaagc caatgaagcc cgcctgggcg aattgctcaa tgaactcgat gcgtttctgg
     1681 cggtgcttcc gaatctgccg caagacagcg ttccggtggg ccgttcggat gtcgacaacg
     1741 tcgaggtgaa acgctggggc gcgccgcgca gctttgcctt cgcggtgaag gatcacgttg
     1801 acctcggcga ggccttgggc cagcttgatt tcgctactgc cgcgaagatc gccggcacgc
     1861 gtttttcgct gatgaagggg caacttgccc gtttgcatcg cgtgctcgct cagttcatgc
     1921 tcgatacgca taccgcgcgg catggctaca ccgaagtcta cgcgccttac ctggtcaacg
     1981 cggccagcct gctgggtacc gggcagttgc cgaagttcga ggaggacttg ttcaaggtcc
     2041 tgcgcgccga cgccgaaccg ttgtacctga ttcccacggc cgaggttccg gtcaccaaca
     2101 tcgtgcgcga cgccattctc acggcggcgg agttgccgct gaagttcgtc tgtcacaccc
     2161 cgtgttttcg ctcggaagcg ggatcatacg gtcgagacac gcgcggcatg atccgccagc
     2221 accagttcga caaggtcgag ctggtgcaga tcgtggccgc cggggattcg ctggcggcgc
     2281 acgaagaact caccggacat gccgaagcca ttctcgaaat gctcgatctg ccttatcggc
     2341 gcgtggcgtt gtgtaccggc gacatcggct tctcttcagc caagacctat gacctcgagg
     2401 tctggctgcc ggcacagaag gcttaccgcg agatctcctc gtgctccaac ttcgaggcat
     2461 ttcaggccag gcgcatgcag gcgcgttacc gcaacgagcg caacaaaacc gaactggtgc
     2521 ataccctcaa tggttccgga cttgccgtcg ggcgcaccct ggtggcggtg atggaaaact
     2581 tccagaacac cgacggcagc atcag
//
LOCUS       DAOSIT010000332         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_34582_length_2592_cov_2.996453, whole genome shotgun sequence.
ACCESSION   DAOSIT010000332 DAOSIT010000000
VERSION     DAOSIT010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34582_length_2592_cov_2.996453"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLL14_08285"
     CDS             <1..763
                     /locus_tag="PLL14_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG03967.1"
                     /translation="RSAVFSQHAFAVYPLATLSTGAELIATPARHYGHDLDAMRAAIR
                     PDTRIVWIANPNNPTGNFLPYPEVRSFLEAVAQDVVVVLDEAYNEYLPPAERVDTAAW
                     IKDFPNLVVTRTFSKIFGLAGLRVGYALASAEIADLMNRVRQPFNVNNLAIAAAIAAL
                     DDHLFVAESYELNRRGMEQLVAGLKRLGLEHIPSHGNFVTFRVGEGARVNQKLLQQGV
                     IVRPIGGYGLPEWLRVTIGSEPENARFLEALEKAL"
     gene            764..1633
                     /locus_tag="PLL14_08290"
     CDS             764..1633
                     /locus_tag="PLL14_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG03968.1"
                     /translation="MPEFGKVVVFGTGLIGGSFALALKEADAVEEVVGFGRTPSTLRI
                     AQELGVIDRAGINPTHEIEDADIVLVATPVAQMLEIFERIAPYLGPNTIVTDGGSTKG
                     DVVAAAREAFGERIRQFVPAHPIAGAENSGPSAARWDLYQGKKVVVTPLPENADDALD
                     HIKRAWSLCGADIYELTPEAHDRVFAAVSHLPHLLSFALVHDLAVREDADLFFTFAAS
                     GFRDFTRIAASHPEMWRDICIANRDALLVELDRYRAQLDDLRLALARNDGERLEETFG
                     LARQARREWADKG"
     gene            1635..2474
                     /locus_tag="PLL14_08295"
                     /pseudo
     CDS             1635..2474
                     /locus_tag="PLL14_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
BASE COUNT          463 a          852 c          817 g          460 t
ORIGIN      
        1 tcgctcggcg gtgttctccc agcatgcctt cgcggtttac ccccttgcca cgctgtcgac
       61 cggagccgaa ctgattgcga ccccggccag gcactacggg cacgacctgg acgccatgcg
      121 tgccgccatc cgtcccgaca cgcgcatcgt ctggatcgcc aatccgaaca acccgaccgg
      181 caactttctg ccctatccgg aagtgcggtc cttcctcgag gcggtggcgc aagacgtggt
      241 cgtcgtcctc gacgaggcct acaacgaata cctgccgccg gccgagcgcg tcgataccgc
      301 tgcctggatc aaggatttcc ccaatctggt cgtcacccgc accttttcga agattttcgg
      361 gctggccgga ctgcgtgtcg gctatgcgct ggcatcggcc gagattgccg acctgatgaa
      421 ccgtgtgcgc cagccgttca acgtcaacaa cctggcaatt gcggcggcca tcgccgcgct
      481 cgacgaccac ctgtttgtcg ccgaaagcta cgaactcaac cggcgcggca tggaacaact
      541 cgttgccgga ctcaagcgcc tcggactgga acacattccc tcgcacggaa actttgtcac
      601 cttcagggtg ggcgaagggg cgcgtgtgaa tcagaagctg ctgcagcagg gcgtcatcgt
      661 caggccgatc ggcggctatg gcttgccgga atggttgcgc gtcaccatcg gcagcgaacc
      721 ggaaaacgcc cgctttctcg aagcgctgga gaaggcgctg tagatgccag aattcggcaa
      781 agtcgtcgtt ttcggcaccg gactgatcgg cggttccttc gcgctggcgc tgaaggaggc
      841 ggatgccgtc gaggaggtgg tcggcttcgg ccgcacgcca tcgacattga ggatcgcgca
      901 ggaacttggt gtcatcgacc gcgccggcat caacccgacc cacgaaatcg aggatgccga
      961 catcgttctg gtcgccacgc cggtggcgca gatgctggag atcttcgagc gcatcgcccc
     1021 gtacctcggc ccgaacacca tcgtcaccga cggcggatcg accaagggcg atgtcgttgc
     1081 cgcggcgcgt gaagcattcg gcgaacggat ccgccagttc gtgccggcgc acccgattgc
     1141 cggcgccgaa aacagcggcc cgtcggcggc gcgctgggat ctctaccagg gcaagaaagt
     1201 cgtcgtcacg cccctgccgg aaaatgccga cgatgcgctc gaccacatca agcgcgcctg
     1261 gtcgctgtgc ggggcggata tctacgaact gacgccggaa gcgcacgacc gcgtatttgc
     1321 cgccgtcagt catctgcccc acctgctgtc gttcgcgctg gttcatgatc tggcggtgcg
     1381 cgaggatgcc gacctgtttt tcaccttcgc cgcctcaggg ttccgtgact tcacccgcat
     1441 cgccgccagc caccccgaaa tgtggcgcga catctgcatc gccaaccgcg acgcgctgct
     1501 tgtcgaactg gacaggtatc gcgcccagct cgacgacctc cgcctggcac tggcacgcaa
     1561 cgatggcgag cgcctcgagg agactttcgg ccttgcccgg caggcacggc gcgagtgggc
     1621 ggacaagggc tgaaatgatc cgtctgttac gcagcctgtt gctggcattc gcgctggtgc
     1681 tcccggccac gccggttcgc gccgatgccc cggccattct cctcgccgag gtctatcgca
     1741 atcaggtcga cgtcgggcag taccttgtca gtgaaaagct cgatggtgtg cgcggcatct
     1801 gggatggcca gaccctgcgc ttccgcagcg gcaagacgat caacgccccc acctggtttc
     1861 tcgatggcct cccgaagcgc cctctggacg gcgaactgtg gatgggccgc ggcaccttcg
     1921 aacgcctctc cggcatcgtc cggcgcgagg tttccgacga tgccgaatgg cggcaggtgc
     1981 gctagatgat cttcgagctg cccggtgcgc ccggcacctt tgcccagcgc gccgagcaga
     2041 ttcgcgaaac ggtcaaacag gcgaacgtgc cgtggctgcg tgaaatcgag cagttccggg
     2101 tggtcgaccc cgacagcctg aaaaaacgca tgaaagaggt cgtcaaggcg ggtggcgagg
     2161 ggctgatgct gcaccgcgcc gacgcgccct acgagacggg ccgcagcgat accctgctca
     2221 agatgaaacc ctgggaagac gccgaagcgg tcgtcatcgg ccacctgccc ggcaagggca
     2281 agcatgccgg ccagatgggc gccttgcgtg tccgcgccga agacgggcgg gaattttcgc
     2341 tgggtagcgg cttcaccgac cgggtacgcc gcgatccgcc agcagtcggc accacggtga
     2401 cctatcgcta ccacgacctg actcggacgg ggttgccgcg cttcgccagc tttctgcggg
     2461 tgcgggtgga ttgatgcgca acagcaaaat gcatttctgg atattggaaa cgaacagatg
     2521 attcttgtca ccggcggtgc cggcttcatc ggcagcaatt ttgtcctcga ctggctggct
     2581 caatgcgacg ag
//
LOCUS       DAOSIT010000333         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_34670_length_2586_cov_5.401817, whole genome shotgun sequence.
ACCESSION   DAOSIT010000333 DAOSIT010000000
VERSION     DAOSIT010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34670_length_2586_cov_5.401817"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..499)
                     /locus_tag="PLL14_08300"
     CDS             complement(26..499)
                     /locus_tag="PLL14_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03969.1"
                     /translation="MLEQDEQVVGDDADAEEGGIGGELSAGDTFHAEADLQFLDAILG
                     SFPALAIPNQGRFGRFDTVADDDMALCCFVEQFGLALVLDDDQAERLLRLAHAMHFFG
                     NGAVGVRLPGGLGNPGNGLLRRRIQSPPKICYTRCACSNSAYARRKISGQSAVVG"
     gene            1079..1900
                     /locus_tag="PLL14_08305"
     CDS             1079..1900
                     /locus_tag="PLL14_08305"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOG03970.1"
                     /translation="MNNHFRSLSIALKLNLIQGAILLIIILAATVWTTSHLRAQLTVK
                     AIDDLKQINRLAVAMLEAYDRSLRSDIERSGRVFSGNFEKQLELAEGGEAPRLLQRGM
                     PLNERLDVVDAFTARSGTVATILVRQGDDFLRTATSLKKEDGSRSTGTLLGNDHPARQ
                     ALLAGKAYTGKASMFGKDFMTHYIPVHDAAGKVAGAFFVGLEFTEGLKALKQSILAIK
                     VGETGYVAALDAGRQKGVATIHPAREGANLLAAKDSRGFEFVREMVEKKAGSIRY"
     gene            1979..>2586
                     /locus_tag="PLL14_08310"
     CDS             1979..>2586
                     /locus_tag="PLL14_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG03971.1"
                     /translation="MSTSDVEEIRRGADTAVSDIAVMAVAIILAALSSGFLVTRHWVT
                     RPLHELVLAADRIAAGDLSGRLSADSGDEVGHLKQAISAMAENLKTTIGNFRDAAATM
                     LEQSLALVTAAEQVNRGSQRQRDAATGMTSSVDTMSDSIDQVARHACEAQRLSDGSGQ
                     AAVQGAEVTRQAVAAMNQITGFVRQASTTVGELGRRSQDISAV"
BASE COUNT          513 a          826 c          797 g          450 t
ORIGIN      
        1 gactggagtt gttgctgcgc gccggtcagc cgaccaccgc cgactggccg gatattttgc
       61 gacgcgcata cgccgaattc gaacaggcgc agcgcgtgta acagatcttc ggtggcgact
      121 ggatgcgccg acgcagcagg ccatttccag ggttcccgag gccgcctgga agacgtacgc
      181 cgactgcgcc gttgccgaaa aagtgcatcg catgggcgag acgaagaagg cgttccgcct
      241 gatcgtcgtc aagtacaagc gccaggccga actgttcgac gaagcaacaa agtgccatgt
      301 catcatcagc aaccgtgtcg aatcgaccga agcgaccttg gtttggtatc gccagcgcgg
      361 ggaaacttcc gagaatggca tcaaggaact gaagatcggc ttcggcatgg aacgtatccc
      421 ctgcggacag ttcgccgcca atgccgcctt cttccgcatc ggcgtcatcg cccacaacct
      481 gttcgtcctg ttcaagcatg gcgcactggg cgccgattgg cagtgccggt gccacaaggt
      541 cgcgaccgtg cgttggcgcc tgtttcatct gcccggcacg gtggtgcgcc atgccgggtc
      601 atgggtgttg agatcgccac cgacaccgtc gatttggttc gcgccatccg cacgcgcagc
      661 tttgcccagg cgctgagcag ttctccgtag ccggcgacca ctgaccacca agttcaaagg
      721 ccaagaccct tccgcgcagc gatgcgcgag aataggctca cctagcaccc gcgcattcat
      781 gccgtagtga tccttcctgc gaagcctccg cgtcgtcagc cacactcaaa ccgatcaccc
      841 ccctcgtatc tcactcctcg gcagctccgt cgcgcccaaa aacctggcca gttcgtcgcc
      901 cccaatttca atcgcgggat tcgggttgct atgcgcgctt gccaaacggc tgcgcgcgga
      961 ggtccgccgc gccgaccgct tcgatcgagg actctcggcg cacgccgccg agccatccct
     1021 gctggcgccg ccgacaccgt agtattattc tgccaacgcc tcgtgaaaga acctgcgcat
     1081 gaacaaccac ttccgcagtc tgtcgattgc cctgaaactc aacttgatcc agggcgcgat
     1141 cctgctcatc atcattctcg cggcgacggt gtggacgacc tcgcatctac gcgcacaact
     1201 gaccgttaag gcgatcgacg atctgaagca gatcaatcgc ctcgcggtag cgatgctcga
     1261 agcctacgat cgcagcctgc gcagcgacat cgagcggagt gggagggtct tttccggcaa
     1321 cttcgagaag caactggaac tggcggaagg cggcgaggcg ccgagactgc tgcagcgcgg
     1381 catgccgctt aatgagcgcc tggatgtcgt cgacgctttt accgctagat ccgggacagt
     1441 agcgacgatt ctggtccgcc agggcgacga tttcctgcgc accgccacct cgctgaagaa
     1501 ggaggatggc agcaggtcta cgggaacgct gctcggtaac gaccatcctg cgcgccaggc
     1561 actgctcgcg ggcaaggcct acaccggcaa ggcctcgatg ttcggcaagg atttcatgac
     1621 ccattacatt ccggtgcacg acgcggccgg caaggtagcc ggcgcctttt tcgtcgggct
     1681 ggaattcacc gaagggctca aggccctgaa gcaaagcata ctggcgatca aggtcggcga
     1741 aaccggttac gtcgccgcgc tcgatgccgg caggcagaaa ggcgtggcga ccatccatcc
     1801 ggccagggaa ggtgccaacc tgctggccgc caaggacagt cgaggattcg agttcgtccg
     1861 cgaaatggtc gagaagaaag ccggcagcat ccgctactag tgggcgaatc caggcgaaac
     1921 gagcgcgcgc gagaatgtcg cggtttttga ccactacccg gcctgggatt gggtattggt
     1981 gtccaccagc gatgtcgagg agatcaggcg tggcgccgac accgcggtca gcgacatcgc
     2041 ggtaatggcc gtggcaatca ttctggcagc cctgtccagc ggcttcctgg tcacccggca
     2101 ttgggtcacc cgtcccctgc atgagctggt gctggcggcc gaccgcattg ccgccggcga
     2161 cctgagcggg cgtctgtctg ccgacagtgg tgacgaggtc ggacacctca aacaggccat
     2221 cagcgcgatg gccgagaatc tcaagacgac cattggcaac ttccgcgacg ccgccgcaac
     2281 gatgctcgaa caatctctgg ctttggtcac tgctgccgaa caggtcaacc ggggttcgca
     2341 gagacagcgc gatgcggcaa ccggcatgac cagttcagtc gacaccatga gcgacagcat
     2401 cgatcaggtc gcccggcacg cgtgcgaagc acagcgcctg tccgacggat ccgggcaggc
     2461 tgcggtgcag ggagccgaag tgacccggca ggctgtcgcg gcgatgaacc agatcaccgg
     2521 tttcgtacgt caggcgtcga cgacggtagg cgaactgggg cgccgctcgc aggacatctc
     2581 ggcggt
//
LOCUS       DAOSIT010000334         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35003_length_2567_cov_10.412022, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000334 DAOSIT010000000
VERSION     DAOSIT010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35003_length_2567_cov_10.412022"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..804
                     /locus_tag="PLL14_08315"
     CDS             91..804
                     /locus_tag="PLL14_08315"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOG03972.1"
                     /translation="MATSTMQFKIYRYDPDKDAAPYMQDISVEVDFAIDRKLLDVLTK
                     LKAKDDTLSYRRSCREGICGSDAMNINGKNGLACLTEIKDLKQPVVLRPLPGLPVIRD
                     LIVDMTQFFKQYHSIKPYLINEGPRPDRERLQSPEEREELNGLYECILCACCSTSCPS
                     FWWNPDKFVGPAGLLNAYRFIADTRDQATNERLDDLEDPYRLFRCHSIMNCVDVCPKG
                     LNPTRAIGKIKEMMVRRAI"
     gene            804..1058
                     /locus_tag="PLL14_08320"
     CDS             804..1058
                     /locus_tag="PLL14_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOG03973.1"
                     /translation="MGDREKLNRLRWRCTRRAMLEMDLLLGEFLDRIFPTLTPEQADA
                     FVNLADMEDLELWPLVNGSRECSDPVQAEVVALLRDVRVK"
     gene            1129..2424
                     /gene="gltA"
                     /locus_tag="PLL14_08325"
     CDS             1129..2424
                     /gene="gltA"
                     /locus_tag="PLL14_08325"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOG03974.1"
                     /translation="MTTERKATLIIEGRAPVEFPIMTPTHGNDCIDIRTLGAKTGLFT
                     YDSGYLSTASCKSKVTFIDGDKGELLYRGYPIEQLAEQCNFLEVAYLLWHGELPTPTQ
                     KVGFEKNIKQHTMVHEQLVKFYQGFRRDAHPMAVLVGVVGALSAFYHEAEDFSDLEHQ
                     NISFNRIVAKLPTIVAMAYKYHRGEPFIYPRNDLDYTANFMHMMFGTPCEEYKPNPVL
                     VHALDTIFTLHADHEQNASTSTVRLSGSSGANPYACISAGIACLWGPAHGGANEACLQ
                     MLEEIHDVSRVGKYIARAKDKSDDFKLMGFGHRVYKNFDPRAKLMRTVCNEVLQELGL
                     ENDRLFKLAMELEKIALEDDYFIDKKLYPNVDFYSGIVQKALGIPTTMFTCIFALART
                     VGWMTQWEEMINDPEYKIGRPRQLYVGTARRDVVPAGHR"
BASE COUNT          561 a          734 c          712 g          560 t
ORIGIN      
        1 tacaagccgg tgatcatgaa gccgctgtcc gtcgaatcca tcgcgctgaa gacgcgttcc
       61 tattgatcga gcgccaagga gtcaatgaat atggccacca gtaccatgca gttcaagatt
      121 taccgctacg atcccgacaa ggacgctgcg ccctatatgc aggacatctc ggtcgaggtc
      181 gatttcgcca ttgatcgcaa gctgctcgat gtgctcacca agctgaaagc caaggatgac
      241 acgctttcgt atcgtcggtc gtgtcgcgag ggtatttgcg gctccgatgc gatgaacatc
      301 aacggcaaga acggcctggc ctgccttacc gagatcaagg acctcaagca gccggttgtc
      361 ctgcggccgc tgccgggctt gccggtgatc cgcgacctga tcgtcgacat gacccagttc
      421 ttcaagcagt accactcgat caaaccttac ctgatcaatg aagggccgcg tccagaccgt
      481 gagcgtctgc agtcgccgga agagcgcgaa gagctcaacg gcctctacga gtgcattctg
      541 tgcgcctgct gttcaacctc atgcccatca ttctggtgga atccggacaa attcgtcggt
      601 ccggcgggat tgctgaatgc ttaccgcttc atcgccgata cccgtgacca ggcgaccaat
      661 gagcgcctgg atgatcttga ggatccttat cgcctgttcc gttgccacag tatcatgaac
      721 tgtgtcgatg tctgcccgaa gggtctcaac ccgacgcgag cgatcggcaa gatcaaggaa
      781 atgatggtgc gtcgcgcgat ctgatggggg acagggaaaa gctcaatcgc ctgcgctggc
      841 gctgcacacg ccgcgccatg ctggaaatgg atctcttgct cggggagttt ctcgaccgca
      901 ttttcccgac cctgactccc gagcaggcag atgcgtttgt caacctggcc gacatggagg
      961 acctggaact gtggccgctg gtgaatggta gccgcgagtg ttcagatccg gtgcaagccg
     1021 aagtcgtggc cttgttgcgc gacgtaagag ttaagtaagg aagttgccat aatctaggcg
     1081 acctcctgtt cgtagctgtg acctcaacac ctgaacgggg ataaaacaat gacgaccgaa
     1141 cgtaaagcaa cgctgatcat cgaagggcgg gcgcccgtcg agtttccaat catgacgcct
     1201 acccatggca atgattgtat cgacattcgt accttgggcg ccaagaccgg tctctttact
     1261 tacgattccg gctacctgtc gacagccagt tgcaagtcga aggtcacgtt cattgacggc
     1321 gacaagggcg aactgctcta tcgcggctat cccatcgagc aattggccga gcagtgcaac
     1381 ttcctggagg tcgcctacct cctgtggcat ggcgaactgc cgacgccgac gcagaaggtg
     1441 ggtttcgaaa agaatatcaa gcagcacacc atggtgcatg agcaactggt caagttctac
     1501 cagggtttcc gtcgggacgc gcacccaatg gccgtgctgg ttggcgttgt cggcgccttg
     1561 tcggcctttt accatgaggc ggaggatttt tccgacctcg agcatcagaa catcagcttc
     1621 aaccggattg ttgccaaatt gccgacgatt gtcgccatgg cctacaagta ccaccgtggc
     1681 gagccgttca tctatcctcg caacgacctc gattacaccg ccaacttcat gcacatgatg
     1741 ttcggcacgc cgtgcgagga gtacaagccg aatccggtgc tggtacatgc actggacacc
     1801 attttcaccc tgcatgccga tcacgagcaa aacgcatcga cctctacggt ccgcctgtca
     1861 ggttcttccg gcgccaatcc ctacgcctgt atctccgcgg gtatcgcttg tttatggggg
     1921 ccggcacacg gtggcgcgaa tgaggcctgc ctgcagatgc tcgaggaaat ccatgatgtc
     1981 tcgcgcgtcg gcaagtacat tgcccgcgcc aaggacaaga gcgatgactt caagctgatg
     2041 ggtttcggtc accgcgtgta caagaacttc gatccacgcg ccaagctgat gcgtacggtt
     2101 tgcaacgaag tgctgcagga acttggcctg gagaatgatc gtttgttcaa actggcgatg
     2161 gaactcgaaa aaatcgccct cgaagacgat tacttcatcg ataagaagct ctatccgaac
     2221 gtcgattttt attcaggtat cgtccagaag gcgcttggca ttccgaccac gatgtttacg
     2281 tgtatcttcg ccttggcacg cacggtcggc tggatgacgc agtgggaaga aatgatcaac
     2341 gatcccgagt acaagatcgg tcgtccgcgc cagctctatg tcggcaccgc tcggcgcgac
     2401 gtggttcctg ccggccaccg ctaggccgac ggatgcaaga gggcggcagg cagtagccgc
     2461 ccttttttca tgctgtgact tttcggccac ggctttcctt aattcgaacc agaacggtga
     2521 cgacaggtaa ctgccatgat gaatcagctt tttggtaact ccctcct
//
LOCUS       DAOSIT010000335         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35051_length_2564_cov_9.533679, whole genome shotgun sequence.
ACCESSION   DAOSIT010000335 DAOSIT010000000
VERSION     DAOSIT010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35051_length_2564_cov_9.533679"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..433)
                     /gene="tolR"
                     /locus_tag="PLL14_08330"
     CDS             complement(29..433)
                     /gene="tolR"
                     /locus_tag="PLL14_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOG03975.1"
                     /translation="MRPRRLKNEINVVPYIDVMLVLLVIFMVTAPMMTTGTIDVPSVG
                     KAAQTPGEALRVDVAADGKLSLVLPGKSEGRRVHLGDLGEAVAAAQKTPEQAVLVAGD
                     KKVNYQAVMDVMAALQKAQVKRIGLLVEQSGR"
     gene            complement(438..1115)
                     /gene="tolQ"
                     /locus_tag="PLL14_08335"
     CDS             complement(438..1115)
                     /gene="tolQ"
                     /locus_tag="PLL14_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOG03976.1"
                     /translation="MNVSQDLSILHLILNASAVVQAVMLLLAGVSFMSWYYIFRKWFT
                     VKAARAQTEQFERDFWSGGDLNSLYQSAINDRHSTGSMERIFEAGFREFTKLRSQKNL
                     DPKDVIDGSRRAMRATYQREMDSIDSHLAFLASVGSVSPYVGLFGTVWGIMHSFRGLS
                     NVGQATLAAVAPGIAEALVATAIGLFAAIPAVVAYNRFAHEIDRLSSRFESFMEEFSN
                     ILQRQMR"
     gene            complement(1112..1555)
                     /gene="ybgC"
                     /locus_tag="PLL14_08340"
     CDS             complement(1112..1555)
                     /gene="ybgC"
                     /locus_tag="PLL14_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOG03977.1"
                     /translation="MKYEQKTNAFTIPVRIYYEDTDAGGVVYYANYLKFFERCRTEWL
                     RAIGHQQAELLRDPGIAFVVRGVAIEFLMPARLDDQLVVGLEVEKISRAQLHFRQHIR
                     RADAHADGGWQELVSGRIHIVCVNSKLMKATSIPAFLRTKLEALQ"
     gene            complement(1632..>2564)
                     /gene="ruvB"
                     /locus_tag="PLL14_08345"
     CDS             complement(1632..>2564)
                     /gene="ruvB"
                     /locus_tag="PLL14_08345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG03978.1"
                     /translation="KIREQLAIFIEAARKRNDTLDHVLLFGPPGLGKTTLAHIIATEM
                     GVNLRSTSGPVLERAGDLAAILTNLEPRDVLFIDEIHRLSPVVEEILYPALEDFQIDI
                     MIGEGPAARSVKLDLPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYTPEELTLIVTR
                     SSQLLKVPTDAGGALEIARRSRGTPRIANRLLRRVRDFAEVRAGGCITPEVADAALLM
                     LDVDHGGLDVMDRKLLFAIIDKFGGGPVGVDNLAAAIGEARDTIEDVLEPYLIQQGFL
                     QRTPRGRVATAAVYRHLGLETLPREPQGALWQTP"
BASE COUNT          484 a          776 c          828 g          476 t
ORIGIN      
        1 ggttcgacag cttccacggc tcaggaactc accggccgct ttgctcgacg agaagaccga
       61 tgcgtttgac ctgcgccttt tgcagcgcgg ccatcacatc catcaccgct tgatagttga
      121 ccttcttgtc gccggccacg agcaccgctt gttcgggagt cttctgcgcc gccgcgaccg
      181 cttctcccag atcaccgaga tgcacgcgcc ggccctcgga cttcccgggg agaaccagac
      241 tcagcttgcc atcggcggcg acgtctactc gcagagcttc cccaggtgtc tgcgctgcct
      301 tgcccaccga aggcacatcg atcgtgccgg tggtcatcat cggtgccgtc accatgaaga
      361 tgaccagcag cacgagcatg acatcgatat agggaacgac gttgatttcg tttttcagac
      421 gacgcggacg catggcctta cctcatctgc cgttgcagga tgttcgagaa ctcctccatg
      481 aacgactcga agcgcgacga gagccgatcg atttcgtgcg caaaacggtt gtaggcgacc
      541 accgccggaa tcgccgcgaa caggccgatg gcggtggcga ccagggcctc ggcaatgccg
      601 ggggcaaccg cggcgagcgt cgcctgcccg acgttcgaga ggccgcggaa ggaatgcatg
      661 attccccaga cagttccgaa caggccgacg taggggctca ccgagccgac cgaagcgaga
      721 aaggcgagat gcgagtcgat actgtccatc tcgcgctggt aggtggcgcg catggcgcgt
      781 cgcgagccgt cgatgacatc tttcgggtcg aggttcttct ggctgcgcag cttggtgaat
      841 tcgcggaagc ccgcctcgaa gatgcgctcc atgctgccgg tgctgtggcg gtcgttgatc
      901 gcgctctggt agaggctgtt caagtcgcca ccgctccaga agtcgcgctc gaactgctcg
      961 gtctgcgcgc gcgccgcctt gacggtgaac cacttgcgaa agatgtagta ccaggacatg
     1021 aacgagacgc cggccagcaa cagcatgacc gcctgcacca cggcactggc gttgaggatc
     1081 aggtgcagaa tcgaaaggtc ttgcgagaca ttcattgcag ggcttccagt ttggtacgca
     1141 gaaatgcggg aatcgaagtg gctttcatca gctttgagtt gacgcaaacg atgtggatgc
     1201 ggccgctgac cagttcttgc cagccgccgt cggcgtgcgc atcggcgcgc cggatgtgct
     1261 ggcgaaaatg caactgcgca cggctgatct tctccacttc cagaccgacg acgagttgat
     1321 cgtcgagtcg cgccggcatc aggaactcga tcgcgacgcc acgaacgacg aaagcaattc
     1381 ccggatcgcg cagcagctcg gcctgctgat ggccgatcgc gcgcagccat tcggtgcggc
     1441 agcgctcaaa aaacttcagg taattggcgt aatagacgac gccgccggcg tcggtgtctt
     1501 cgtaataaat acgcaccgga atggtgaacg cgttggtttt ttgctcgtat ttcatggccg
     1561 gcgccgattc taaggcagcc cccaggaggc cactgtaagg gtttgtgact ggtcgccgag
     1621 gcaccacggg ttcagggggt ttgccacaac gcaccctgtg gttcgcgtgg caatgtctcg
     1681 aggccgaggt gacggtagac ggctgccgtc gccacgcgcc cgcgcggcgt acgttgcagg
     1741 aagccctgct ggatcagata gggttcgagc acatcctcga tcgtatcgcg cgcctcgccg
     1801 atggcggcgg cgaggttatc gacgcctacc gggccaccgc cgaacttgtc gatgatggcg
     1861 aacaacagtt tgcggtccat gacgtcgagt ccgccgtgat cgacgtcgag catcaacagc
     1921 gccgcatccg cgacctccgg cgtgatgcag ccgccggccc gtacctcggc aaaatcgcga
     1981 actcgccgca gcaggcgatt ggcgatgcgt ggcgtgccgc gcgaacggcg ggcgatttcc
     2041 agcgcgccgc cggcatcggt gggcaccttg agcagttgcg aagagcgggt gacgatcagc
     2101 gtcagttcct cgggcgtgta gaactcgagc cgcgagacga tcccgaagcg gtcgcgcagc
     2161 ggattggtca acatgccggc acgggtcgtc gccccgacca gtgtgaaggg cggcaggtcg
     2221 agtttgacag agcgcgccgc gggaccctca ccgatcatga tgtcgatctg aaagtcctcg
     2281 agcgccggat aaagaatctc ttcgacaacc ggcgagagac ggtggatctc gtcgatgaac
     2341 agtacgtcgc ggggttcgag gttggtcagg atggcggcga ggtcgcccgc gcgttcgagt
     2401 accgggcctg aagtcgaacg caggttgacc cccatttcgg tcgcgatgat gtgcgccagt
     2461 gtggttttcc ccagccctgg cgggccgaac aggagcacgt gatcgagcgt gtcgttgcgc
     2521 ttcctggcgg cctcgatgaa gatcgcgagt tgctcgcgaa tctt
//
LOCUS       DAOSIT010000336         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35052_length_2564_cov_9.349143, whole genome shotgun sequence.
ACCESSION   DAOSIT010000336 DAOSIT010000000
VERSION     DAOSIT010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35052_length_2564_cov_9.349143"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLL14_08350"
     CDS             <1..545
                     /locus_tag="PLL14_08350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG03979.1"
                     /translation="TEERVQHLAHHDQLTDLPNRVLFSDRLFQAIAQARRDRGTLALM
                     FLDLDRFKPVNDSLGHDIGDLLLREVALRLQACAPRSSDTVSRLGGDEFVILLAQIDK
                     AMDAVVVAGKVLAALGRPFRIGPHQIEISASIGIAVYPQHGDDVHELLKNADTAMYHA
                     KKAGRNCYRFFREINTEAAV"
     gene            complement(579..2075)
                     /locus_tag="PLL14_08355"
     CDS             complement(579..2075)
                     /locus_tag="PLL14_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG03980.1"
                     /translation="MPNVHCPPLRIRSPRRSGALLAVLAWLSGCASQPASDGAAIAHW
                     TQLGARGEISLRAIVAASDGCPKAVVDGVTLPLLTRAGATPLATDRNVANGNPAFEAA
                     FAVNSCELTLPGSARQASIGGQAVALPGTDIRRIVVVGDTGCRIKVPASGSGDPIQDC
                     NSPVDWPWQRVAAAAARTRPDLVIHLGDYHYREYCDQPALCGPLRERGVVIDYGWSGW
                     NADFFVPAKPLLNAAAWIVVRGNHEDCDRGGEGWMRFLSPVPYQACANQLYKTDSRSV
                     LDNNRTAEAYAIDLGDALSVVVIDNAGHEDYRPASKAAQDVAVFKRTLAALQTVPETQ
                     QIWLLLHRPIWYDLLEAPAQPNALQLALARQMPANVQFAFAGHQHAFQTINFAPESDT
                     VNYPAGRPAQVVVGASGTQLEAFDPSSPLYQGRSGSDSRERAHPDGRLYDGVAAGSGI
                     VLNRYSFLLLERDDAGWAGTLLDPDGKSISRCRLDGDRKEIACSVQDP"
     gene            complement(2115..>2564)
                     /locus_tag="PLL14_08360"
     CDS             complement(2115..>2564)
                     /locus_tag="PLL14_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HOG03981.1"
                     /translation="AIFGYLGGHSQNAALLYKNEAAIQKTAASNQWNYYQAKSNKQNL
                     AELSITLTSGEAREKYAQEVERYKKEKQEIKVEADKLEAVAKAADQKSELEMHVHERW
                     ALATTLLQVAIAMAAITLLTRKRWMLFGVYGATALGLLAGTAGYLHL"
BASE COUNT          440 a          822 c          807 g          495 t
ORIGIN      
        1 ccaccgaaga gcgtgtgcag cacctggccc atcacgacca gttgaccgac ctgccaaacc
       61 gtgtactgtt cagcgaccgc ctgtttcagg ccatcgcgca ggcgcgccgc gaccgaggta
      121 ccctggcact gatgttcctc gatctggacc gcttcaaacc ggtcaatgac agccttggcc
      181 acgacatcgg cgatctgttg ctgagggaag tcgcgctccg cctgcaggct tgtgcgccga
      241 gatcctcgga taccgtgtcg cgactcgggg gcgatgagtt cgtcattctg ctggcgcaga
      301 tcgacaaggc gatggatgcc gtggtcgtcg ctggcaaggt tctggctgcg ctcggtcgac
      361 ccttccggat cggtccccat cagattgaaa tttcggccag catcggcatc gcggtctatc
      421 cgcaacatgg tgacgacgtg cacgaactgc tcaagaacgc ggacaccgcc atgtaccacg
      481 ccaagaaagc gggtcgcaac tgctaccgct ttttccggga aatcaacacc gaggctgccg
      541 tctaatccgt cagccgacct gtcaggccgc ccagcccatc acgggtcctg gacgctgcag
      601 gcgatctcct tgcggtctcc atcgagtcgg caacggctga tgcttttccc atcaggatcg
      661 agcagggtac ccgcccagcc cgcatcgtca cgctccagca acagaaagct gtagcgattg
      721 aggacgatgc cgctgccggc ggcaacgccg tcgtagagtc gcccgtcggg atgcgccctt
      781 tcccggctgt ctgagccact cctgccttga taaagggggg aagacggatc gaaagcttca
      841 agctgcgtgc cactggcgcc gaccacgact tgcgctggcc gtcctgccgg gtagttgacg
      901 gtgtcgcttt cgggcgcaaa gttgatcgtt tgaaaggcgt gctggtgacc ggcgaaggcg
      961 aactggacgt tcgccggcat ttgccgtgcc agggcaagct gcaaggcgtt gggctgcgcc
     1021 ggcgcttcga gcaggtcgta ccagatcggg cgatgcagta acagccagat ctgctgcgtt
     1081 tccggaaccg tctgcagtgc cgccagggtg cgcttgaaga ccgcgacatc ctgtgctgcc
     1141 ttgctcgccg gacgatagtc ctcgtgtccc gcgttgtcga tgacgaccac gctgagcgcg
     1201 tcgccaaggt cgatggcata ggcttcggca gtgcggttgt tgtcgagcac cgagcggctg
     1261 tccgtcttgt acaactggtt cgcgcaagcc tggtaaggca ccggggaaag gaagcgcatc
     1321 cagccttcgc caccgcgatc acaatcttcg tggttaccgc gcacgacgat ccacgcggcg
     1381 gcgttcagca gcggcttcgc cgggacaaag aaatcggcat tccagccgga ccaaccgtag
     1441 tcgatgacca ctccccgctc gcgtagcggc ccgcagagtg ccggctggtc gcaatattca
     1501 cggtagtggt aatcgccgag atgaatcacc agatcgggac gcgtgcgcgc cgcggctgcg
     1561 gccacccgct gccagggcca atcgaccgga ctattgcaat cctggatcgg atcgcccgaa
     1621 ccgctggccg gaaccttgat tcggcagccc gtgtctccga ccaccacgat gcgtcgaatg
     1681 tcggtgccgg ggagcgccac ggcctggccg ccgatgcttg cctgacgtgc cgatcctggc
     1741 agggtgagtt cacaggaatt caccgcaaac gcggcctcga atgccgggtt cccgttcgcc
     1801 acgttgcggt ctgttgccag cggcgtggcg ccggcgcgcg tcagcagggg aagcgtgacg
     1861 ccatcgacga ccgccttcgg acacccgtca ctggcggcaa cgatcgcccg caggctgatc
     1921 tccccacgcg cgcccagttg cgtccagtgc gcgattgcgg cgccgtcgct ggctggctgg
     1981 ctggcgcagc ctgacagcca ggcgagcacc gccaacagtg cgccgctacg gcgtggagag
     2041 cgtattctga gcggcgggca gtgtacgttt ggcatcggtg tgcatctcct gaatactgca
     2101 gcctgcagcg tggatcagag gtgaaggtaa ccggccgtgc cggccagcag tccgagagcg
     2161 gtcgcaccat aaacaccgaa cagcatccag cgcttgcgcg tcagcaaggt gattgcggcc
     2221 atcgcgatgg caacctggag cagggtggtc gccagagccc agcgttcgtg cacgtgcatc
     2281 tccaactcgc tcttctggtc agccgctttg gcgaccgctt cgagcttgtc ggcttcgacc
     2341 ttgatctcct gcttttcctt tttgtagcgc tccacttcct gtgcgtattt ctcgcgcgct
     2401 tcgccgctgg tcagggtgat cgacagttcg gccaggttct gcttgttgct cttggcctgg
     2461 tagtagttcc actggttcga ggctgccgtc ttctggatcg ccgcctcgtt cttgtagagc
     2521 agggccgcat tctgcgagtg gccgccgaga tagccaaaaa tggc
//
LOCUS       DAOSIT010000337         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35154_length_2558_cov_9.236117, whole genome shotgun sequence.
ACCESSION   DAOSIT010000337 DAOSIT010000000
VERSION     DAOSIT010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35154_length_2558_cov_9.236117"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..974)
                     /gene="atpG"
                     /locus_tag="PLL14_08365"
     CDS             complement(111..974)
                     /gene="atpG"
                     /locus_tag="PLL14_08365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOG03982.1"
                     /translation="MPSGKEIRNKIKSVESTRKITKAMEMVAASKMRKAQDRMRAARP
                     YGDKIRRVAGNLSHALTDYRHPFLTVRQQVKAVGLITVTSDKGLCGGLNTNVLRLALG
                     KMKEWDAEGKKLRVTAIGNKGLGFMQRAGANVVSQLTGLGDTPHLEKLIGPVKVQLDA
                     YMKGETDVLYIAFTRFINTMKQEPTLYQLLPLSGELVGGEGNRWDYIYEPDAKVVIDD
                     LLVRYVEAMIYQSVAENMASEQSARMVAMKSASDNAKNVIGELKLVYNKARQAAITKE
                     LSEIVGGAAAV"
     gene            complement(993..2531)
                     /gene="atpA"
                     /locus_tag="PLL14_08370"
     CDS             complement(993..2531)
                     /gene="atpA"
                     /locus_tag="PLL14_08370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOG03983.1"
                     /translation="MQLNPSEISELIKSKIENLAISADLRTQGTVVSVTDGICRVYGL
                     TDVMQGEMLEFPGNTFGMALNLDRDSVGAVVLGEYDEISEGDTVKTTGRILEVPVGPE
                     LLGRVVNSLGQPIDGKGPVNNKLTDKIEKVAPGVIWRKSVSQPVQTGLKSIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGQNLICIYVAIGQKASTVANVVRKLEENGAM
                     AYTIIVAATASESAALQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSEAWVEKFTNGEVKGKTGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLDTDLFNAGVRPAIDAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLDRGRLVTELMKQPQYSPLSISEMAITLHA
                     VNKGLFDDVDVKKALATEKQMQGFVKQKYADLITKIETSKDLDADAEQKLTKAIEEFK
                     ATLA"
BASE COUNT          488 a          767 c          727 g          576 t
ORIGIN      
        1 tgggcattgc gtcacgcggg aactggatgt ccaccacggc gccgatacac tgaacaatgt
       61 ttccttgact catcgtcgta tccctaatca ataaactaac tatcgcgtgg tcagacagcc
      121 gctgcgccgc cgacgatttc cgaaagctct ttggtgatcg ctgcctggcg agccttgttg
      181 tacaccagct tgagttcgcc gatcacattc ttggcgttgt ccgaagcgga cttcatcgca
      241 accatgcgcg cgctctgctc ggaagccata ttttcggcga ccgactggta gatcatcgct
      301 tcgacataac gcaccagcaa atcgtcgatc accaccttgg catccggctc atagatgtag
      361 tcccatcggt tgccttcgcc accaacgagt tcgccggata gcggcaacag ctgataaagg
      421 gtcggctcct gcttcatggt attgatgaaa cgcgtgaatg cgatgtacag aacatccgtt
      481 tcacccttca tgtacgcgtc gagctgcacc ttgaccggtc cgatcagttt ttccaggtgc
      541 ggcgtatcgc cgagtccggt caactgcgag acgacgttcg caccggcccg ctgcatgaac
      601 cccagtccct tgttaccgat cgcggtaacg cgaagcttct tgccttcggc atcccactcc
      661 ttcatcttgc caagtgcaag acgcaagaca ttggtattca ggccaccgca gagccccttg
      721 tcagaggtta cggtgatcag tccgactgct ttgacctgct ggcgtacggt caggaaggga
      781 tggcggtagt cggtcagggc atgcgagaga ttgccggcaa cacgccggat cttgtcgccg
      841 tagggacggg cggcgcgcat cctgtcctgc gccttgcgca tcttggatgc ggccaccatt
      901 tccatggcct tggtgatctt gcgcgtgctc tcgacgctct tgatcttgtt gcggatttcc
      961 ttgccgctag gcatggcaat ctcctcgatc gatcaggcca gcgtggcctt gaattcctca
     1021 atcgccttgg tgagcttctg ctctgcgtcg gcatcaagat ccttggacgt ttcgatcttg
     1081 gtgatgaggt cagcgtactt ctgcttgacg aagccttgca tctgcttctc ggtggccagc
     1141 gctttcttca cgtcaacatc gtcgaagagc cctttgttca ccgcatgcag agtgatcgcc
     1201 atctcggaga tcgacaacgg cgaatactgc ggctgcttca tcagctcggt caccagccgg
     1261 ccacgatcga gctgcttgcg cgtcgcttcg tcaaggtcgg acgcaaactg ggcgaaggcc
     1321 gccagttcac ggtactgggc aagggccaga cgtacaccac caccgagttt cttgatgact
     1381 ttcgtttgcg ccgctccgcc gacccgcgag acagaaatac ctgcgtcgat agcgggacgc
     1441 acaccggcgt tgaagaggtc ggtgtcgagg aagatctggc cgtcggtaat cgagatcacg
     1501 ttggtcggaa cgaaggcgga cacgtcaccg gcctgcgtct cgatgaccgg cagggcagtc
     1561 aatgaaccgg tcttgccctt gacttcgcca ttggtgaatt tttcgaccca ggcttccgag
     1621 acgcgcgctg cgcgctcaag caggcgggag tgcaggtaga agacgtcacc tgggtaagct
     1681 tcacgccccg gtggacgacg cagcagcagc gacacctggc ggtaagccca cgcctgcttg
     1741 gtcaagtcgt catagatgat cagggcgtcc tggccgcggt cgcggaaata ctcgcccatg
     1801 gtgcatccgg aatacggagc gatgtactgc agggccgcgg attccgaagc ggttgcggcg
     1861 acaatgatgg tgtacgccat cgcgccattc tcttccagtt tgcgcaccac gttggcaacc
     1921 gtcgaggcct tctgaccgat cgccacatag atgcagatca ggttctgacc cttctggttg
     1981 atgatcgtgt cgaccgctac cgcagtcttg ccggtctgcc ggtcaccgat gatcagttcg
     2041 cgctggccac gaccgatcgg aaccatcgcg tcgatcgatt tcaaaccggt ctgtacgggt
     2101 tgcgaaacag atttgcgcca gatgactcca ggcgcgacct tctcgatctt gtcggtcaac
     2161 ttgttattga ccgggccttt gccgtcgatc ggctgcccca gcgagttcac gacgcgaccg
     2221 aggagttccg gtccaaccgg aacctcgagg atgcggccgg tggtcttgac cgtgtcgcct
     2281 tcgctgatct cgtcgtactc accgagaacg accgcgccaa ccgagtctcg gtcgaggttc
     2341 aaggccatgc cgaaagtgtt gcccggaaac tcgagcattt ctccctgcat gacgtcggtc
     2401 aggccgtaga cacggcagat accgtcggtc accgaaacga cggtgccctg ggtgcgcaga
     2461 tcggcgctga tcgcaagatt ttcaatcttg ctcttgatca gttcgctgat ttcggaagga
     2521 ttcaactgca tggaaattct cctagttctt gagcgcca
//
LOCUS       DAOSIT010000338         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35193_length_2555_cov_8.646400, whole genome shotgun sequence.
ACCESSION   DAOSIT010000338 DAOSIT010000000
VERSION     DAOSIT010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35193_length_2555_cov_8.646400"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLL14_08375"
     CDS             <1..455
                     /locus_tag="PLL14_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03984.1"
                     /translation="ARIDALRGENDVAKYLLLAGKLRNHELTLSPEQQVNWRRLAALV
                     APAVRTLVHLGEVVNELTAESNALSEQVKAVIDAREDSCSGVMCAVDKVEGETRVSAV
                     MVRLADTPLSALPGKDLKVRLRRTDAATKLLFAGAGGHFEWSYRTPSK"
     gene            complement(498..>2555)
                     /gene="cphA"
                     /locus_tag="PLL14_08380"
     CDS             complement(498..>2555)
                     /gene="cphA"
                     /locus_tag="PLL14_08380"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOG03985.1"
                     /translation="QFGWGSRQRRIQAAETDATSAVAESIAQDKELSKILLHAAGVPV
                     PCGRPVLSAEDAWAAACEIGCPVVVKPQDGNQGKGVAVNLSTREQIEAAYAIAFEVSD
                     EVLVERFLPGHDYRLLVVGDKLIAAARRDPPLVIGDGVHSIGQLVERVNSDPRRGEGH
                     ATSLTRIRLDDLALARLANAGLAPDSVPARGQRVVLRNNANLSTGGTATDVTADVHPE
                     FAAQAVAAAQTIGLDIAGVDVVCDSVLRPLEAQGGGIVEINAAPGLRMHLQPSFGKGR
                     PVGEAIIANMFAPGDDARIPLVAVAGTNGKTTTVRLIAHILAQSGLRVGMTTTDGVYI
                     RGRRIDTGDCSGPKSAKNVLLHPDVDAAVLETARGGVLREGLGFDRCDVAVVTNIGLG
                     DHLGLSYISTVEDLSVVKRVIVQNVRPGTGVAVLNAADPMVARMADSCPGGVTFFAAD
                     RHHPVMARHRAQRKRVVYRDGDSIVASGGGVEHRIALADIPLTQHGAIGFQVENAMAA
                     TAATWALGVSWEATHAGLRSFVNDAQTAPGRFNVFSYRGATLIADYGHNPDAIQALVD
                     AIDNLPSSVGSQRSVVISGAGDRRDEDIRQQTEILGHAFDRVVLYQDQCQRGRADGEV
                     LALLQEGLKNASRTTEIREIRGEFLAIDTALAELQPGDLCLVLIDQIEDALEHIGRRV
                     AEG"
BASE COUNT          428 a          837 c          850 g          440 t
ORIGIN      
        1 gcgcccggat cgacgccttg cgcggcgaga acgatgttgc caagtacctg ctgcttgccg
       61 gcaaactgcg aaaccacgaa ctgaccctga gtccggaaca gcaggtgaac tggcgcagac
      121 tggcggcact ggtcgctccc gccgtgcgca ccctggtgca tctcggagaa gtggtcaacg
      181 aactgacggc cgagtcgaat gcgctgagcg agcaggtcaa ggcggtcatc gatgccaggg
      241 aggattcgtg tagcggcgtg atgtgcgccg tcgataaggt tgaaggcgaa acacgtgtca
      301 gtgccgtcat ggtgcggctc gctgataccc cactcagtgc cctgccgggc aaggacttga
      361 aggtacgttt gcgacgcacc gacgcggcga ccaaactgct ctttgccggt gctggcgggc
      421 acttcgagtg gtcctatcgc acgccgtcga aatagcggcg gcacaggcac ggccggagga
      481 ggtgacgcct gccgccctca gccttcggcc acgcgtctgc cgatgtgttc aagggcatcc
      541 tcgatctggt cgatcaggac caggcaaaga tcgccgggtt gcaattcggc cagcgccgtg
      601 tcgatggcca ggaactcgcc acggatctcc cggatctccg tggtccgact ggcgttcttc
      661 aggccctcct gcagcagggc gagcacttcc ccatccgctc ggccacgctg gcactggtcc
      721 tgatagagca cgacccgatc gaaggcgtgc cccagaattt ccgtctgctg gcggatgtcc
      781 tcgtcgcgcc ggtcaccggc gccgctgatc accaccgagc gctggctgcc cacgcttgat
      841 ggcaggttgt caatggcgtc gaccagggcc tgaatggcgt ccgggttgtg gccgtagtcg
      901 gcaatcagcg tcgcgccccg ataagaaaag acgttgaagc gtcccggcgc ggtttgcgcg
      961 tcgttcacga aactgcgcag gccggcgtgc gttgcctccc aggacacccc gagcgcccag
     1021 gtggcggcgg ttgccgccat ggcgttctcg acctggaaac cgatggcgcc gtgttgtgtg
     1081 agcggaatgt cggccaatgc gatacggtgt tcgaccccgc ctccggaagc gacgatcgag
     1141 tcgccgtcac gatacacgac acgcttccgc tgtgcgcggt gcctggccat taccgggtga
     1201 tgtcggtcgg cagcaaaaaa ggtgacgccg cctgggcacg aatcggccat ccgggcgacc
     1261 atcggatcgg ccgcattgag caccgcaacg ccggtcccgg gccggacgtt ctggacgatc
     1321 acacgcttga ccaccgacag atcctcgacg gtgctgatgt aggaaagtcc cagatgatca
     1381 cccagaccga tgttggtgac taccgctaca tcgcagcggt cgaagccaag cccctcgcgc
     1441 agcacgccgc cgcgcgccgt ctcgagcacg gcggcgtcga cgtcagggtg gagcaggaca
     1501 ttcttggcgc tcttcgggcc gctgcagtcc ccggtatcga tgcgtctgcc ccgaatgtac
     1561 acgccgtcag tcgtggtcat tccgacccgc agaccgctct gcgcaagaat atgggcgatc
     1621 agccggaccg ttgtcgtctt gccgttggtg ccggcgactg ccaccagcgg gatgcgggcg
     1681 tcgtcaccag gcgcaaacat gttggcgatg atcgcttctc ccaccggccg tcccttgccg
     1741 aaggacggct gcagatgcat gcgcagtccc ggcgcggcgt tgatttcgac gatgccgcca
     1801 ccctgggcct caagcgggcg aaggacgctg tcacacacga catcgacgcc ggcgatatcg
     1861 aggccgatgg tctgtgccgc ggccaccgcc tgcgccgcga attcgggatg gacatcggcg
     1921 gtgacatcgg ttgccgtgcc tcccgtcgag agattggcgt tgttgcgcag caccacacgc
     1981 tgacctcttg ccggtaccga atccggcgcc agccccgcat tcgccaggcg cgccagggcc
     2041 aggtcatcga gacggatgcg ggtcagcgag gtggcgtggc cttcgccgcg tcgaggatcg
     2101 ctgttgaccc gctcgaccaa ctggccgatg ctgtgcacgc catcgccgat gaccagcggc
     2161 gggtcgcggc gggcggcggc gatgagtttg tcgccgacga ccagcaaacg gtagtcgtgg
     2221 cccggcagga aacgctcgac gagcacttcg tcgctgactt cgaaggcgat cgcgtaggcg
     2281 gcttcaatct gttcgcgggt gctcaggttg acggccacgc ccttgccctg gttgccatcc
     2341 tgcggtttga cgacgaccgg gcagccgatt tcgcacgccg ccgcccaggc gtcctcggca
     2401 ctgaggacgg gccggccgca gggaaccgga acgccggccg cgtgcaggag gatcttgctc
     2461 agttccttgt cctgcgcgat cgattcggcg acggcgctgg tggcatcggt ctcggcagcc
     2521 tggatgcgac gctgccggct gccccagcca aactg
//
LOCUS       DAOSIT010000339         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35209_length_2554_cov_9.048820, whole genome shotgun sequence.
ACCESSION   DAOSIT010000339 DAOSIT010000000
VERSION     DAOSIT010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35209_length_2554_cov_9.048820"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLL14_08385"
     CDS             complement(<1..287)
                     /locus_tag="PLL14_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG03986.1"
                     /translation="MTTFKSLAVENFVAFHKRLNWEPHASLNVIIGENDTGKTHLLKL
                     LYSMARAIEYFGRKKAGPEPKELSELLAHKLRWAFLPQKMELGRLVTRGSG"
     gene            complement(336..1541)
                     /gene="pglX"
                     /locus_tag="PLL14_08390"
     CDS             complement(336..1541)
                     /gene="pglX"
                     /locus_tag="PLL14_08390"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-2 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HOG03987.1"
                     /translation="MTLPNRQNLDAARSQSARLRRQMIAAQEELDWRCYRLYSLLPAG
                     SAAADFEHPTPPEVALGERAFEIVLARRVAAGQETTTWFERHGSTPITEIPGHWPEDY
                     RGVVQRRIALIESDRNIGLIERPEYKRRWNSPAWESLEQAALRDWLLARLESPRYWAT
                     SAEQPPRMTSTSRLADALQHDAEFMQIAELYAGHADFHTAQLVAELVASEAVPFLPVL
                     RYTDSGLRKRAQWEDTWSLQRREDAGEDLASSVGRIPVPPKYQSKDFLKSDFWRLRGG
                     LDVPKERWISYPGCERGADDSLVIAWAGWDHLQQATALATYFIDRKEREGWPRERLQP
                     LLAGLLELLPWLKQWHNDMNPDFGARMGDYYASFVAEEARALQFTLDDLRAWKPAATA
                     ARRGRKKAG"
     gene            complement(1538..1957)
                     /locus_tag="PLL14_08395"
     CDS             complement(1538..1957)
                     /locus_tag="PLL14_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOG03988.1"
                     /translation="MKVVIDTSVLVSAVIRDRLPERVLRWCLGNESVIWLVSPAILAE
                     YLKVIRRPRFALSAELIGWWSELLLSDTLMTQITAAPDFPRDPKDASFLACAGANGAD
                     FLITGDADFTEARALLPTVRIVSVREFAQIVGLEPGA"
     gene            complement(1954..2193)
                     /locus_tag="PLL14_08400"
     CDS             complement(1954..2193)
                     /locus_tag="PLL14_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03989.1"
                     /translation="MQAYKTYARIQATGELAIAHLPFAPGALVEVLVVGAERSAAERE
                     QEWARMMQAVQALPQSPKISDKDIAAEIDASRSGL"
     gene            complement(2198..2461)
                     /locus_tag="PLL14_08405"
     CDS             complement(2198..2461)
                     /locus_tag="PLL14_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03990.1"
                     /translation="MQAIELETTISPQGGIVLPADYKALYGRHARVILLLADDPETAG
                     PAHRRSPPPQFAGKVRELGEVMSSVSPAEWGIEERSYPTNPKE"
BASE COUNT          411 a          804 c          861 g          478 t
ORIGIN      
        1 ttgccgctgc ctcgcgtgac cagccgcccc agctccattt tctgaggcag gaacgcccac
       61 cgcagcttgt gcgccagaag ctcggagagt tccttgggct ccggtcccgc cttcttccgg
      121 ccaaagtatt cgatcgcgcg cgccatcgaa taaagcagct tcagcaagtg cgtcttgccg
      181 gtgtcgttct cgccaatgat gacgttcagc gacgcatggg gttcccaatt cagccgcttg
      241 tggaaagcta cgaagttctc aacagcgaga gatttgaatg tagtcatcaa cggctcctgt
      301 tgcttgtcgg tttctgcttc cgaatctcca tggattcagc cagccttctt tcgcccacgc
      361 ctggctgccg tagccgctgg cttccaggcg cgcagatcat caagcgtgaa ttgcagcgcg
      421 cgcgcttctt cggcgacgaa gctcgcgtag tagtcgccca tgcgcgcgcc gaaatcgggg
      481 ttcatgtcgt tgtgccactg cttgagccag gggagcagtt cgagcaggcc ggcgagcagg
      541 ggctgcaggc gctcgcgtgg ccagccttcg cgttccttcc tgtcgatgaa gtacgtcgcc
      601 agcgcggtcg cctgctgcag gtgatcccag ccggcccagg cgatgaccag gctgtcgtcg
      661 gcgccacgtt cgcagccggg gtagctgatc cagcgctcct tgggcacgtc gagaccgccg
      721 cgcaggcgcc agaagtccga cttcaggaag tccttgctct ggtacttggg cggcaccggg
      781 atcctgccga cgctgctggc caaatcctcg ccggcgtctt cgcggcgctg tagcgaccag
      841 gtgtcttccc actgcgcgcg cttgcgcagg ccgctgtcgg tatagcgcag caccggcagg
      901 aaaggaaccg cttccgacgc caccagttcg gcgacgagct gggcggtgtg gaagtcggcg
      961 tggccggcgt acagctcggc aatctgcatg aactcggcgt cgtgctgcag cgcgtcggcg
     1021 aggcggctgg tgctggtcat ccgcggcggc tgctcggcgc tggtcgccca gtagcgcggc
     1081 gactcgagcc gcgccagcag ccagtcgcgc agcgccgcct gttccagcga ttcccacgcg
     1141 ggcgagttcc agcggcgctt gtactcgggg cgctcgatca ggccgatgtt gcggtcgctt
     1201 tcgatcaggg cgatgcgccg ctgcacgacg ccgcggtagt cttcgggcca gtggccgggg
     1261 atctcggtga tcggtgtcga gccgtggcgc tcgaaccagg tggtggtttc ctggcccgcc
     1321 gcgacgcggc gggccaggac gatctcgaaa gcacgttcgc cgagtgccac ttcgggtggc
     1381 gtggggtgct cgaaatcggc tgcggcgctg ccggccggta gcaggctgta aaggcggtag
     1441 cagcgccagt cgagttcttc ctgcgcggcg atcatctggc ggcgcaggcg ggcggattgg
     1501 ctgcgggcgg cgtcgaggtt ctggcggtta ggcagggtca tgctccgggc tccagaccga
     1561 caatctgggc aaattcacgc acgctgacaa ttctgactgt cggcaagagt gctcgcgctt
     1621 cagtaaaatc ggcgtcgccg gttatcagga aatcggcgcc attggcgcca gcgcaggcca
     1681 ggaacgatgc atccttggga tcgcgcggga aatcgggcgc ggcggtgatc tgagtcatga
     1741 gcgtatccga cagcaacagc tccgaccacc agccgatcaa ctcggccgac agcgcgaaac
     1801 gcggacggcg gatgactttc agatactcgg ccagaatcgc tggcgagacg agccaaatca
     1861 ccgattcgtt gccgaggcac cagcgcaata ctcgttccgg cagacggtcg cggatgaccg
     1921 ccgacactag cacgctggta tcgatcacga ccttcatagg ccagaccggc tggcgtcgat
     1981 ttctgcggca atgtctttgt cgctgatctt cggtgactgc ggcagcgcct gcaccgcctg
     2041 catcatgcgc gcccattcct gctcgcgctc ggcggcgcta cgttcggcgc caacgaccag
     2101 gacttccacc agagcccccg gcgcaaacgg caggtgagcg atggccagtt cgccagtggc
     2161 ttgaatgcgg gcgtaggtct tgtaggcttg catcaggtta ttccttcggg ttggtggggt
     2221 acgagcgttc ctcgattccc cactcggcag gagaaacgga cgacatgact tcgccaagct
     2281 ccctgacctt gccggcgaat tgcggcggcg gcgaacggcg gtgagccggc ccggcggtct
     2341 cagggtcgtc cgccagcagc aggatcacgc gcgcgtggcg gccgtagagc gccttgtagt
     2401 cggcgggcaa cacaatgccg ccttgcggag aaatggtggt ttcgagttca atggcttgca
     2461 tgtcaggacc cctttattcc cggcgggtat gaggccgatc ctggccgtgg ccaggttccg
     2521 ggtcggggcg ggacgacgcg agcaacgccg ccgg
//
LOCUS       DAOSIT010000340         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35261_length_2551_cov_8.564103, whole genome shotgun sequence.
ACCESSION   DAOSIT010000340 DAOSIT010000000
VERSION     DAOSIT010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35261_length_2551_cov_8.564103"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLL14_08410"
     CDS             complement(<1..472)
                     /locus_tag="PLL14_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG03991.1"
                     /translation="MDILITPVTPADVAPVTALARLVWQQTYPGIISGEQIDFMLEQR
                     YNPQRLREELTMPSLWWDQARVEGQLAGFSSCLLTAAGREMKLDKLYVDPARQRLGIG
                     ARLIAQVARRAVQARCAALILAVNKRNERAIAAYRKHGFVVRESVCVDIGNGFVM"
     gene            complement(655..1476)
                     /locus_tag="PLL14_08415"
     CDS             complement(655..1476)
                     /locus_tag="PLL14_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03992.1"
                     /translation="MSTPKNRSATPIQQPFAPVNPQPRQHSRASHWPDGLPPQVRRIL
                     DTPVPVFVALQKEAPAPGAQPLTHVGFVLDRSTSMETGKSATIEGFNTQVNVVKAGAR
                     DAGETRLTMVDFATEARLRYVGLAPDALEPLNEANYIPDGWTALYDGIGLIIAELLAQ
                     PGMDSPLTATLVTIFTDGEENRSSLYSAAILRELVQKLEASGRWTFALVGPRHGVAAL
                     ARLLAIDPSNTTGFNPASEADRVDTQIRMASASASYMSSRSFGHTQMSGLYDEEG"
     gene            complement(1473..1931)
                     /locus_tag="PLL14_08420"
     CDS             complement(1473..1931)
                     /locus_tag="PLL14_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03993.1"
                     /translation="MADDESTKPPGRPRLYASASEKLSAFRARLEQAGYVRREVLVTR
                     GTEERVKALAEELNVKPLDVYSAMVAYGLDAYQREHGATEVTLSGSSTAQHAQASGPF
                     MSGGEARRLAQETDRSLAAAPGMPGSGPASPVRTNNPIAAFFQKRKETLK"
     gene            2326..>2551
                     /locus_tag="PLL14_08425"
     CDS             2326..>2551
                     /locus_tag="PLL14_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG03994.1"
                     /translation="MRFLARLLSVVVPLSLMIGGPSQAREWEAIKSSGTIVAATEGGF
                     QPFNYYDGPKLTGFEVELAEAIAKKLGLKLE"
BASE COUNT          454 a          789 c          817 g          491 t
ORIGIN      
        1 ccatcacgaa tccgttgcca atatcgacgc agaccgattc gcggaccacg aagccatgtt
       61 tcctgtaagc ggcgatggcg cgttcattgc gcttgttgac cgccaggatc agcgccgcac
      121 agcgtgcctg cacggcccgg cgggccacct gcgcgatcag tcgcgcaccg atacccaggc
      181 gctgccgcgc cggatcgacg tagagcttgt cgagcttcat ttcacggccg gcggcggtca
      241 acagacacga cgagaaaccg gccagttgac cctcgacccg ggcctgatcc caccacaacg
      301 acggcatcgt caattcctcg cgcaagcgct gcgggttgta gcgctgctcg agcatgaagt
      361 cgatctgctc gccgctgatg attccagggt aggtctgctg ccagaccagg cgcgccagcg
      421 ccgtgaccgg cgcgacatcg gcaggggtga ctggcgtgat caggatgtcc atggcggctc
      481 cggtaggttc gtcaccgcgc ggccgacgca tgcggcaggc atgccggatc ggcagcgcgc
      541 ggtgacgggg gcaaaaagag acgaattgta gccggtgatc gccggttttc gctgtcgatg
      601 cggcagcatc gaacccctgc cctggccggc gacgatcgcc ggcgcgccac cctctcagcc
      661 ttcctcgtcg taaagcccgc tcatctgtgt gtgaccgaac gagcgagacg acatgtagct
      721 ggcactggcg ctggccattc tgatctgcgt gtcgacccga tcagcctccg atgccgggtt
      781 gaaaccggtc gtattggacg ggtcgatggc cagcaaacgc gccagggccg ccacgccgtg
      841 cctggggccg accagggcaa aagtccatcg accgctggct tcaagcttct ggaccagttc
      901 cctgagaatc gccgcagagt acaaggagga gcggttttcc tccccatcgg taaaaatggt
      961 gaccagggtc gcggtcagcg gcgagtccat cccgggctgc gccaggagtt cagcgatgat
     1021 caggccaatg ccgtcgtaca gcgccgtcca cccgtccggg atgtaatttg cttcattcag
     1081 cggctccagc gcatccggag ccaggccaac gtaacgaagg cgtgcttcgg tggcaaaatc
     1141 gaccatcgtc aggcgcgtct ctccagcgtc ccgggcaccc gccttgacta cattcacctg
     1201 ggtgttgaaa ccttcgatcg tcgccgactt gccggtctcc atcgaggtcg agcggtccag
     1261 gacaaagccc acatgcgtca ggggttgcgc accaggcgcc ggggcttcct tttgcagcgc
     1321 aacgaacacc gggacgggcg tgtccaggat gcggcggact tgtggcggca acccgtccgg
     1381 ccagtgcgaa gcgcggctgt gctggcgggg ttgcgggttg accggagcaa atggttgctg
     1441 gatgggggtg gctgaacggt tctttggggt gctcatttga gagtctcctt gcgtttctgg
     1501 aaaaacgcgg cgatgggatt gttggtacga accggcgaag cagggccgct gccgggcatg
     1561 cccggagcgg cggcgaggga tcgatcggtt tcctgcgcga gccggcgggc ctcgccaccc
     1621 gacatgaagg ggcctgatgc ctgcgcatgc tgcgcagtag acgatccgga aagtgtcact
     1681 tccgtagcgc cgtgttcgcg ctgataggcg tcgagaccgt acgcgaccat tgccgagtaa
     1741 acgtccaggg gcttgacgtt cagttcctcg gccaaggcct tgactcgctc ctcggtgccg
     1801 cgggtcacca gaacttcccg gcgcacatag ccggcttgct ccaggcgagc ccgaaacgcc
     1861 gacaacttct ccgaagcact cgcatagagg cgcgggcgcc ctgggggttt ggtggattcg
     1921 tcatcggcca cgcaggcttc ctttttagtt accggtaacc aataatatcg gtaccggtaa
     1981 ctaaatgcaa tcgaaaaatg acgacgacca caacagcatg gtctcggaag gcgacatccg
     2041 gcaattcatc ggggatgccg gctatccgac gatctggcga cgactcgcgt ggcccttcag
     2101 ccggactcgc ccgtagccgg tgcctcccgc ttgctgtccg gagttgctgc gctgtgccag
     2161 tcgcagccga gcgagcccgt tgcggcagtt accgtttttc ttgcgccctg tgccccggtt
     2221 tgttgtactc ttggctactt ttttgccgaa ctggccgggt ttgcacgggt cgaggggtgt
     2281 cggcgcttgt tcaacgaggt ctgatcttta tcaacaggag gagtcatgcg tttcctagcc
     2341 aggttgttga gtgtcgtcgt tccactgtcg ctgatgatcg gcggtccgtc ccaggcccgc
     2401 gagtgggagg cgatcaaatc ctccggcacc atcgttgccg cgaccgaggg gggctttcag
     2461 ccgttcaact attatgacgg tcccaaactg accggtttcg aagtggaact cgccgaagcc
     2521 atcgccaaga agctcggcct gaagctcgaa t
//
LOCUS       DAOSIT010000341         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35321_length_2547_cov_10.316613, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000341 DAOSIT010000000
VERSION     DAOSIT010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35321_length_2547_cov_10.316613"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLL14_08430"
     CDS             <1..654
                     /locus_tag="PLL14_08430"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOG03995.1"
                     /translation="IVLAIIEMANKLDIMTVAEGVETPEQMAFLETSGCDVIQGYHFS
                     RPLPLAALTDFAEHPPYEVASKPRIKKPLAEASNPLLAWSFTYETGHAWIDAQHQELI
                     RILSRFDASTRSGDKKSEPRKVMKALIEYVRSHFSFEEKLMLEHAYPDIGEHQAQHLQ
                     LADALASYDLQLKANPRINLQQLVMSLHAWLMQHIHSSDKKLGKFLKKRGISALPGQ"
     gene            742..909
                     /locus_tag="PLL14_08435"
     CDS             742..909
                     /locus_tag="PLL14_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG03996.1"
                     /translation="MDMPITNQNGGYGTLLAGVNAELGKTLRLGLGASTTIGQPGTRS
                     SAINVTLSAPF"
     gene            complement(945..1214)
                     /locus_tag="PLL14_08440"
     CDS             complement(945..1214)
                     /locus_tag="PLL14_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOG03997.1"
                     /translation="MNKRINPEQIDNENPEWTDEDLKHARSFAGLPESLQAKLRGRPP
                     AAVTKERITIRLSREVVEQFRASGDGWQTRMDVALKDWLKTHSPA"
     gene            complement(1192..1467)
                     /locus_tag="PLL14_08445"
     CDS             complement(1192..1467)
                     /locus_tag="PLL14_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOG03998.1"
                     /translation="MDITYDADKNAGNIALRGLSFERAAAFDFHTAVFTVDDRHDYGE
                     IRHRALGLLEGRLHALVFVETATGIRIISFRKANKREVRRYEQANQP"
     gene            complement(1564..>2547)
                     /locus_tag="PLL14_08450"
     CDS             complement(1564..>2547)
                     /locus_tag="PLL14_08450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOG03999.1"
                     /translation="ANRDITRRKQMEDALREKEEFFRLIAENLEGFVAVLDVEGRRVY
                     NSPSYTRLLGERNLSGTSSFVDIHPADRDRVTRAFRDAVTTGVGQHLEFRFLRADGGI
                     LFMESRSGVIRDSTGRPKRIVVVSHDVTARNEAEGRIHHLAFYDALTQLPNRLTLKDR
                     LSQCMAASKRSGCYGALMFLDLDNFKPVNDTHGHEVGDLLLIEAADRLRKCIREIDTV
                     ARFGGDEFVVLLGGLNADKAESAAQAENVAEKIRLALSEPYLLTGMHEGEPAETIEHH
                     CTASIGVALFLNHEGSRDDLLKWADAAMYQAKEAGRNRIRFHGSPERVARR"
BASE COUNT          505 a          816 c          736 g          490 t
ORIGIN      
        1 atcgtgctgg cgatcatcga gatggccaac aagctcgaca tcatgaccgt cgccgagggt
       61 gtggaaacgc cggaacagat ggccttcctc gaaaccagcg gttgtgatgt catccagggc
      121 taccatttct cgcgtccgct gccgctggcg gcgttgaccg actttgcaga acacccgcct
      181 tacgaggtcg cctcgaagcc acgcatcaag aaaccgctcg cggaggccag caaccccttg
      241 ctggcgtggt cattcaccta cgagacggga catgcctgga tcgatgccca gcaccaggaa
      301 ctgatccgca tcctcagccg atttgacgcc agcacgcgtt caggcgacaa gaagagcgaa
      361 ccgagaaagg tgatgaaggc gctgatcgag tacgtgcgca gccacttttc cttcgaggaa
      421 aaactgatgc tcgaacacgc ttatccggac atcggtgagc accaggcgca gcacctgcaa
      481 ctggccgacg cgctggcgag ctacgacctg caactcaagg ccaaccccag gatcaacctg
      541 caacagttgg tgatgtcgct gcacgcctgg ctgatgcagc acatccattc cagcgacaag
      601 aaactcggga aattcctgaa gaaacgcggg atatcggcgc tcccggggca gtaggctcgc
      661 tgcgcccaac tcacttgtga ctacgaacac gacgacgacc agcacagcga ccgtgccggc
      721 ttcgtcggcg gtccggccgc catggacatg ccgatcacca accagaacgg cggctacggc
      781 acgctgctgg ccggcgtcaa tgccgaactc ggcaagaccc tccgccttgg ccttggcgca
      841 tcgaccacga tcggtcagcc cggaacgcgc agttcggcga tcaacgtcac cttgagcgca
      901 ccgttctgat ccgttttctg cctgcaccgg acgtgggcct gcccttacgc cggcgagtgg
      961 gtcttgagcc aatccttcag cgccacatcc atgcgcgtct gccagccgtc gccactggcg
     1021 cggaactgct ccaccacctc gcgcgacagg cggatggtaa tgcgctcctt ggtcacggca
     1081 gcaggcggac ggccgcgcag ttttgcctgc aaggattccg gaagaccggc aaaagaccta
     1141 gcatgcttga ggtcctcgtc ggtccattcc gggttctcat tgtcgatctg ttcagggttg
     1201 attcgcttgt tcataccgtc gcacctcgcg tttgttcgcc ttgcggaagc tgatgatgcg
     1261 gatacccgtc gcggtttcca cgaacaccag ggcatgcagg cggccctcaa gcaggccaag
     1321 tgcacgatgg cgaatttcgc cgtaatcgtg ccggtcatcc accgtgaaga ccgccgtatg
     1381 gaaatcaaac gccgccgccc gctcgaaact caacccgcgc aacgcgatgt tgccggcgtt
     1441 cttgtctgcg tcgtaagtga tgtccattcg atttattgta tgcacaacaa aaagggacgt
     1501 caaatcggtc agcccggaac gcgcagttcg gcgatcaacg tcaccttgag cgcaccgttc
     1561 tgatcagcgt cgcgccactc gctcaggcga tccatgaaag cgaatccggt tccgcccagc
     1621 ctccttggct tgatacatcg ccgcatccgc ccacttgagt aggtcgtcac ggctgccttc
     1681 gtgattgaga aacaaggcca ctccgatgct tgccgtacag tgatgctcga tggtctctgc
     1741 aggctccccc tcgtgcatgc cggtgagcag atacggctcc gacaaggcca ggcgtatctt
     1801 ttccgcaacg ttctccgcct gggcagccga ttcggccttg tccgcattca ggccaccgag
     1861 caacaccacg aactcgtcgc cgccgaagcg tgccacggtg tcgatctcgc ggatgcattt
     1921 ccttagccga tcggccgcct cgatcaacag caagtcgccc acttcatgcc cgtgcgtgtc
     1981 attgaccggc ttgaaattgt ccagatccag gaacatcagc gcgccatagc agccgctgcg
     2041 cttgctggcc gccatgcact ggctgagacg atccttcagc gtcaggcggt tgggaagttg
     2101 ggtgagtgcg tcatagaagg ccaggtgatg gattcttcct tcggcttcgt tgcgcgccgt
     2161 gacgtcatgc gagacgacga cgatgcgttt gggtcgcccg gtgctgtccc ggatcacacc
     2221 gctgcgcgat tccatgaaca ggattccgcc gtcggcccgc aggaagcgaa attcgagatg
     2281 ctgcccgacc cccgtggtca cggcgtcgcg aaacgccctg gtgacccgat cccggtcggc
     2341 gggatggatg tccacgaatg aagaggttcc ggaaaggttg cgctcaccga gcagacgcgt
     2401 gtaggaagga ctgttgtaga cccgccgtcc ttcgacatcg agcaccgcaa cgaagccttc
     2461 gaggttttcg gcaatcaggc ggaagaactc ctccttctcg cgcaaggcgt cttccatctg
     2521 cttgcgtcgg gtgatgtcgc ggttggc
//
LOCUS       DAOSIT010000342         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35587_length_2533_cov_8.765133, whole genome shotgun sequence.
ACCESSION   DAOSIT010000342 DAOSIT010000000
VERSION     DAOSIT010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35587_length_2533_cov_8.765133"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="trbL"
                     /locus_tag="PLL14_08455"
     CDS             complement(<1..538)
                     /gene="trbL"
                     /locus_tag="PLL14_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HOG04000.1"
                     /translation="MSMAAVGKVAIVPTLLWLVLFSIDAQAAIDNAGVLDNVLARYAA
                     AASAWAGVITARASFLFWTLAVISMVWTFGMLALRKADLGDFYAEFFRFTVFTGFFWW
                     LLTNGPRFATDIMDSLRTLGGMATGTGATLTPSGIVDIGFDIFFKVLDQSSLWSPVDS
                     AAGILISAAILIILALIGV"
     gene            complement(547..774)
                     /gene="trbK"
                     /locus_tag="PLL14_08460"
     CDS             complement(547..774)
                     /gene="trbK"
                     /locus_tag="PLL14_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion lipoprotein TrbK"
                     /protein_id="HOG04001.1"
                     /translation="MTTSRPALLTLVSLVAALISACSPEPPKQEMPAVNDENCKPERI
                     AQVEDKGAREQFASACLRRGPGFQPSPKKEW"
     gene            complement(790..1593)
                     /gene="trbJ"
                     /locus_tag="PLL14_08465"
     CDS             complement(790..1593)
                     /gene="trbJ"
                     /locus_tag="PLL14_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HOG04002.1"
                     /translation="MKVLTSKPTAAKVSATGTLLRALLLALFAGPAATTPAHAAGIPV
                     IDTANLSQTVISSIEAVAQTLKQIQQYQTQLQQYENMLQNTLAPAAYIWDSATGTMNQ
                     LRSAIDTLNYYKTNLGSIDAYLGKFQDASYYRGSPCFRQGGCTDAEWAAIKENQRLGS
                     EAQKRATDALFRGLDQQQAALEADARTLQRLQASAQSATGQMQAISYANQLASQQANQ
                     LLQIRALLVAEQNAIATRNQVLADREAQQAAAGEQLRQGRYVASPVRNW"
     gene            complement(1612..>2533)
                     /locus_tag="PLL14_08470"
     CDS             complement(1612..>2533)
                     /locus_tag="PLL14_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HOG04003.1"
                     /translation="MVAPRSAGSAPGAAGQTGAPGSREETLARLAALRQQSEAAVHDD
                     PTAAYKARLATLQGAGIGGAGAASPQLLPTAAVGGRNNVAQFGKDSSGEPGDRWKLDS
                     RLEAPRSPFELRAGFVIPGTLISGINSGLPGQIMAQVAQDVYDTPTGKHLLIPQGSRL
                     VGRYSSDIAYGQARVLVAWQRIVFPDGKAIDIGAMPGADGVGYAGFTDQVNNHYLRLF
                     GSALLMSAVTAGITYSQGQNQGNSVYGAPTASSAMSEALGQQLGQVTAQLIAKNMNIA
                     PTLEIRPGYRFNVIVARDMTFSKPYQSFDY"
BASE COUNT          413 a          778 c          841 g          501 t
ORIGIN      
        1 tcacgccgat caacgccagg atgatgagga tagcggcgct gatgaggatg ccggccgcgc
       61 tgtcgacggg cgaccacagc gacgactggt cgagcacctt gaagaagatg tcgaagccaa
      121 tatcgacgat gcccgacggg gtcaacgtgg cgcctgtccc ggtggccatg ccgccaagtg
      181 tgcgcaggga atccatgatg tcggtggcga agcgcgggcc gttggtgagc agccaccaga
      241 agaagccggt aaacaccgtg aaccggaaga actcggcgta gaagtcgccg aggtcggcct
      301 tgcgcaaggc cagcatgccg aaggtccaga ccatcgagat cacggcaagc gtccagaaca
      361 gaaagctggc tcgcgccgtg atgacgcccg cccaggcgct ggccgcggcc gcataacgcg
      421 ccaggacgtt atcgaggacg cccgcgttgt cgatcgctgc ctgggcgtcg atggagaaga
      481 gcaccagcca caacagcgtc ggaacgatgg caaccttacc gacggcggcc atgctcatcg
      541 gcgagctcac cattctttct ttggactcgg ttggaatccg gggccgcgac gcagacacgc
      601 cgaggcgaac tgctcgcgcg cgcctttgtc ctcgacctgg gcgattcttt ccggcttgca
      661 gttttcgtca ttcacggccg gcatctcctg cttgggaggc tccggcgagc aggcgctgat
      721 caaggcggcg acgagggaga cgagggtcag aagggcagga cggctggtgg tcatgtctgt
      781 tcctttcgtt taccagttgc ggaccgggct ggcgacgtag cgcccctgac gcagttgctc
      841 gccggcagcg gcttgttgtg cttcccgatc ggcaagcacc tgattgcggg tggcgatggc
      901 gttttgctcg gctaccagca gggcgcgaat ctgcagtagt tggttggcct gctggctggc
      961 cagctggttc gcgtagctga tcgcctgcat ctggccggtc gcgctctgcg cactcgcctg
     1021 gaggcgctgc aatgtcctgg cgtccgcttc cagagcagcc tgttgctggt ccagacccct
     1081 gaacagcgca tcggtggctc tcttctgtgc ttctgaaccg aggcgctgat tctccttgat
     1141 cgccgcccac tcagcgtcgg tgcaaccgcc ttgcctgaag cagggcgaac cgcggtaata
     1201 ggacgcgtcc tggaactttc cgaggtaggc gtcgatgctc cccaggttgg tcttgtagta
     1261 attcagcgtg tcgatcgcac tgcgcagctg gttcatcgtg ccggtggccg agtcccagat
     1321 ataggctgcc ggcgccagtg tgttctgcag catgttttcg tactgctgca gttgcgtctg
     1381 gtactgctga atctgcttca gcgtctgggc gaccgcttcg atggaactga tgaccgtctg
     1441 cgataggttc gcggtgtcta tgaccgggat acctgccgca tgcgctggtg tcgtcgccgc
     1501 cgggccggcg aacagtgcca gaagcagggc ccggagcagg gtgcctgtgg cacttacctt
     1561 tgcggcggtt ggtttgctgg tcaagacttt catgctcact gctccttggc gtcagtagtc
     1621 aaacgattgg tacggcttgg aaaacgtcat gtccctggcg acaatgacat tgaagcgata
     1681 ccctggccgg atctccagag ttggtgcgat gttcatgttc ttggcgatca attgcgcggt
     1741 gacctggccg agttgctggc ccagcgcttc gctcatcgcg ctgctggcgg tcggtgcgcc
     1801 atagacactg ttgccctggt tctggccctg gctgtaggtg atcccggcgg tcacggcaga
     1861 catcagcagg gccgatccga acaggcgcag gtaatggttg ttcacctggt cggtgaagcc
     1921 ggcatagccg acaccatcgg cgccgggcat ggcgccgatg tcgatcgcct tgccgtccgg
     1981 gaaaacgatg cgctgccagg cgacgagcac acgcgcctgg ccgtaggcaa tatcgctcga
     2041 gtagcggccg accaggcgcg agccttgtgg aatcaacaga tgtttgccgg ttggcgtgtc
     2101 gtacacgtct tgcgcaacct gggccatgat ctggcccggc aacccggagt tgatcccgga
     2161 aatcagcgtt cccgggatga cgaatccggc ccgcagctca aacggcgagc gcggcgcttc
     2221 cagcctggag tccagcttcc agcgatcgcc tggctcgccg ctgctgtcct ttccgaattg
     2281 cgcgacgttg ttcctgccgc ccacggccgc ggttggcagc aattggggag acgcggcgcc
     2341 ggcgcctccg atgccggcac cttgcagtgt cgccagccgg gccttgtagg ctgccgtcgg
     2401 gtcgtcgtgg accgcggcct cgctttgctg tcgtagggct gccagccgcg ccagcgtttc
     2461 ttcccggcta cccggtgcgc cggtctgacc tgcagcgcca ggcgccgagc ccgcgctacg
     2521 cggggcaacc atc
//
LOCUS       DAOSIT010000343         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35757_length_2524_cov_4.730255, whole genome shotgun sequence.
ACCESSION   DAOSIT010000343 DAOSIT010000000
VERSION     DAOSIT010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35757_length_2524_cov_4.730255"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..556
                     /locus_tag="PLL14_08475"
     CDS             47..556
                     /locus_tag="PLL14_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOG04004.1"
                     /translation="MRLLISDANILIDMEAGALMETLFQLPMQFGIPDLLYYEEIEPG
                     SPGLENRGLQIMEVSGDFVAYAQQLPGQHNHLLPAKIGPKPSHNDYLALALAKQEACT
                     LLTGDTNLRAVANKEQVNVMGTIGLLRAMIENQLLTVDDAFKALDRMKSGKRRLPWPD
                     AEKVLNALR"
     gene            complement(600..1763)
                     /locus_tag="PLL14_08480"
     CDS             complement(600..1763)
                     /locus_tag="PLL14_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOG04005.1"
                     /translation="MKLPEDVRQTLARRFQSKHREWLVGDAGESQWPLEVTLGVPTEQ
                     AALRQVDGVRAWVSAWQGWQGVGTLSWCDRRWKALGVQRLPEKLALRGPEDVAMWAGE
                     SARWERAQSRYQALIVRWPVLAQQLPRYFDVLADYGDADYQRLAEMLDWIAKYPQSGL
                     YPRQLPVSGLDSKWLDGRKGLLTDLVAVIQEDSSGDLDFYRRCGLKAPPLLVRMRVLD
                     QALRAHVGGVGDITAPVDDLAGISWPVSHVFIVENLQTGLAMSDMPGTVVFMRLGYNV
                     DVLARLPWLARAKCIYWGDLDTHGFAILHRARSYIPELKSVLMDEETLLRHQALWVDE
                     AAQHSAAELTLLTEEEQQLYRDLKQQRWGQNVRLEQERIDWTAAWNVLQRSLA"
     gene            complement(1750..>2524)
                     /locus_tag="PLL14_08485"
     CDS             complement(1750..>2524)
                     /locus_tag="PLL14_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOG04006.1"
                     /translation="RCVIKYAANTIEIVDRAHLERMDLVNDSLGQVPFNQSANQRTYL
                     HIDASDRQLVDVKEFKQEIQQALSLAWTEDREFAEARFLGLRRLVDRLASQDPEQKRW
                     RETVLDVRQHVEFIGREVDESGVEVEIYRSGAGKSGGQRQKLATTCLAAALRYQLGGN
                     DHGVPMYAPVVLDEAFDKADNEFTALAMNIFTNFGFQMVVATPLKSVMTLEPFIGGAC
                     FVDISDRRVSGVLLIEYDSDRQRLKLPEHAREEASVEAS"
BASE COUNT          540 a          795 c          690 g          499 t
ORIGIN      
        1 atcccgatga tgcgcttcca caaagaacgc cagttggagt catcggatgc ggttgctcat
       61 cagtgatgcc aacatcttga ttgacatgga agcaggagcg ttgatggaga cgctctttca
      121 gcttccgatg cagttcggca tccctgatct gctgtactac gaagaaattg aaccgggcag
      181 tccgggtctt gaaaaccgcg gcttgcagat catggaggtg agcggcgact tcgtggcgta
      241 tgcccagcag ttgccaggcc agcataacca tctgcttccc gcgaaaatcg gtcccaagcc
      301 cagtcacaac gactatttgg cactggcgct tgcgaagcag gaggcctgta ccctgctgac
      361 gggcgacacc aatttgcggg ctgttgccaa caaggagcaa gtcaacgtca tgggcaccat
      421 tgggctcctg cgcgccatga ttgaaaacca gttgctgaca gtcgacgacg ccttcaaggc
      481 actcgaccga atgaaatcag gcaagcgacg gctgccctgg ccagatgccg aaaaggtgct
      541 caacgcgctg cgctgatcag gggtcgccat cggcagccaa ctgctgcagg ccagtggctc
      601 taggccaatg accgttgcaa cacgttccat gcggcagtcc agtcgatccg ctcctgctcc
      661 aagcgaacgt tttgccccca gcgctgctgc ttgagatccc gataaagttg ctgctcctcc
      721 tcggtcagga gcgtcagttc cgctgctgaa tgctgcgccg cttcatccac ccacagtgcc
      781 tggtgtcgaa gcaaagtctc ttcgtccatg agcacggatt tcaactctgg aatgtatgaa
      841 cgggcgcgat gcaggatggc aaatccatgg gtatccaagt caccccagta gatgcacttc
      901 gcacgagcca gccaaggcag ccgggccaac acgtcgacgt tatagccaag acgcatgaaa
      961 acgacggtgc ccggcatgtc tgacatcgcc agtccggttt ggagattctc gacaatgaac
     1021 acatgcgaga caggccaact gatacctgcg aggtcgtcaa ccggcgcagt gatgtcaccc
     1081 acgcctccga catgagctcg caacgcctga tccagcaccc tcatgcgcac aaggagcgga
     1141 ggtgctttca gtccgcagcg ccggtagaaa tccagatcgc cggatgagtc ctcttgtatt
     1201 acagccacca gatccgtcag cagccccttg cgaccgtcga gccatttgct gtccagcccg
     1261 gacacgggca gctgacgagg atagaggccg gattgtggat acttcgcaat ccaatccagc
     1321 atttctgcca gcctttgata gtcggcatcg ccgtagtcag ccaggacatc aaagtacctc
     1381 ggtaactgct gcgccagtac cggccagcgg acaatgagcg cctggtagcg agactgggca
     1441 cgctcccatc gagcagactc acctgcccac atggcaacat cctctgggcc gcgcaacgcc
     1501 agcttctccg gcagtcgctg gacacccagc gctttccacc gccggtcaca ccaggatagg
     1561 gtgccgaccc cttgccaacc ttgccatgcg ctcacccaag cacgaacgcc atcgacttgt
     1621 ctcagtgcag cctgctccgt cggtaccccg agcgtaactt ccagtggcca ttgactctcg
     1681 cccgcatcac cgactagcca ctcgcggtgt ttgctctgga agcgacgggc cagggtctgc
     1741 cggacatctt caggaagctt caacgctggc ctcctctcga gcatgctctg gcagcttcaa
     1801 ccgctgacgg tcactgtcgt actcaatcag caggacgcct gacacgcgtc ggtcgctgat
     1861 atccacgaag caggcaccac cgatgaatgg ttcgagggtc atgacggact tcaacggcgt
     1921 agcaaccacc atctgaaatc cgaaattggt aaagatattc atcgccaaag cagtgaactc
     1981 gttgtcggcc ttgtcgaaag cctcatcgag cacgacgggc gcgtacatcg gcacgccgtg
     2041 gtcgttccca cccagctgat accgcaaagc agcggccaag cacgtggtgg ccagcttctg
     2101 ccgttgaccg ccagacttgc ctgctccgct gcggtagatc tcgacctcga cgccgctttc
     2161 atcgacttcc cgtccgatga actcgacatg ctgccgaacg tcgagcacag tctcacgcca
     2221 gcgcttttgc tcggggtcct gactggcgag tcgatcaacc agtcgacgca ggcccaggaa
     2281 ccgcgcttcg gcaaactcgc ggtcttccgt ccacgcaagg ctcagagcct gctggatttc
     2341 ctgcttgaac tccttgacgt cgacaagctg ccggtcgctg gcgtcaatgt ggagataggt
     2401 gcgttgattg gcgctttggt tgaacggcac ttggccaagg ctgtcgttga ccagatccat
     2461 ccgctccagg tgagcacggt caacaatctc aatggtattt gccgcgtatt tgatcacgca
     2521 gcgt
//
LOCUS       DAOSIT010000344         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35814_length_2521_cov_6.703163, whole genome shotgun sequence.
ACCESSION   DAOSIT010000344 DAOSIT010000000
VERSION     DAOSIT010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35814_length_2521_cov_6.703163"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="PLL14_08490"
     CDS             <1..1665
                     /locus_tag="PLL14_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HOG04007.1"
                     /translation="IDRFDAAFFNISPTEAELMDPQHRLFLETVWSALEHAGYRPGAL
                     PASTGVYVGVSATDYATLLAAHAVPADAYTSTGTAHSMLANRVSFFLDVHGPSQAIDT
                     ACSSSLVALHRAVESIRSGQCAMAIAGGVNVALTTDTFVGAHMAGMLSPDGQCQTFSA
                     RANGYVRGEGVAALVLKPLLQAERDGDAILGVLLGSAENHGGRAHSLTAPNARAQAEL
                     VERAMRGIDPGSISYIETHGTGTSLGDPVEINGLKLAFARLHAEFGGAASGQRCGLGS
                     VKSNIGHLEAAAGIAGVVKVLLAMRHRLLPASLHCDPLNPYIDLAGSPFHIVSAACAW
                     EAPAPRRAGVSSFGFGGANAHVVLEEYPLRPTAPPGTPGPWLVVLSARSDERLRAVVG
                     NLCRWIEGAAPAADLLERIAYTLQVGREPMSERLALAVGSPEELCHRLRAFLDGRADV
                     AQVLRGSVKRSEGGPPLPAEGDPQALLRHWIAGRDVTWEGLYRQQPRRIALPTYPFAP
                     DRYWIPTVAPEVDVARLLDEVLDEVVSVDAAIDALRRAGYGQSEQI"
     gene            1675..>2521
                     /locus_tag="PLL14_08495"
     CDS             1675..>2521
                     /locus_tag="PLL14_08495"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG04008.1"
                     /translation="MDRLQAILHDLKSGRIDKAEALRRLEAGRARPAAAVSLLAMDWR
                     EQRLGTPCGENRQRLLWACEQLGPVDAATPLCAGQADAVVRFETYAAQLLAALKDLPV
                     HGPEVLVQVAVPAAQETLFGLSGLLLTAQLENPRLRGQVIGLEGVEDMATVLRDEGGQ
                     ADALVRYRAGVRSVMVSEECALAPAGHPWRDGGVYLITGGAGGLGMLFARDILRHAGQ
                     ATVILAGRSSQPAQRLPASLHYRQIDVADATAVSAGVRAIREEFGTLNGILHCAGVLR
                     DGYLRH"
BASE COUNT          366 a          850 c          885 g          420 t
ORIGIN      
        1 atcgaccgct tcgacgccgc cttcttcaat atctcgccca ccgaagccga gctgatggac
       61 ccgcagcacc ggctgtttct ggagacggtg tggagcgcgc tcgaacacgc cggctaccgt
      121 cccggtgccc tgccggcaag caccggcgtc tacgtcgggg tgtcggcgac cgactacgcg
      181 acgctgctgg cggcgcatgc ggtgccggcc gacgcctaca cctcgaccgg caccgcccac
      241 tcgatgctgg ccaaccgggt ttccttcttc ctcgacgtgc acggcccgag ccaggccatc
      301 gacaccgcct gttcaagttc gctggtcgcg ctgcaccgcg ccgtcgagag cattcgcagc
      361 ggccaatgcg caatggcgat tgccggcggg gtcaacgtcg cgctcaccac cgacaccttc
      421 gtcggcgccc acatggcggg catgctgagc cctgacggcc agtgccagac cttctcggcg
      481 cgtgccaacg gctatgtgcg cggcgaaggc gtggccgcgc tggtgctcaa accgctgctt
      541 caggccgaac gcgacggcga cgccatcctc ggcgtgctcc tcggcagtgc cgaaaaccac
      601 ggtggccgtg cccattcgct gaccgcgccg aatgcccggg cgcaggccga actggtcgaa
      661 cgcgccatgc gtggcatcga tcctggcagt atcagctaca tcgaaacaca cggcaccggc
      721 accagcctcg gcgacccggt cgagatcaat ggtctgaaac tcgctttcgc gagactgcac
      781 gccgaattcg gcggcgccgc cagcgggcag cgctgtggtc tgggttcggt gaaatcgaac
      841 atcggtcacc tcgaagcggc agccgggatt gctggcgtgg tcaaggtcct gctggcgatg
      901 cggcaccgcc tgctgccggc cagcctgcac tgcgatcccc tcaatccgta catcgatctg
      961 gcgggcagcc cttttcacat cgtcagcgca gcgtgcgcct gggaggcgcc ggcaccgcgc
     1021 cgcgccggcg tcagcagttt cggtttcggt ggcgcgaacg cgcatgtggt gctcgaggaa
     1081 tatccgctcc ggcccaccgc gccgccgggc actccgggac cgtggctggt ggtgctctcg
     1141 gcgcgcagcg acgagcgctt gcgcgcggtg gtcgggaatc tgtgccggtg gatcgagggc
     1201 gccgcgcccg cggccgatct gctcgaacgg atcgcgtaca cgctgcaggt cggtcgcgaa
     1261 ccgatgtcgg agcggcttgc gctggcggtt ggctcgccgg aggaactgtg ccaccggctg
     1321 cgcgccttcc tcgacggccg ggccgacgtc gcgcaggtgt tgcgcggctc ggtcaagcgc
     1381 tccgaaggcg gcccgcccct gccggccgag ggcgacccgc aggccctgct gcggcactgg
     1441 atcgctgggc gcgacgtcac ctgggaaggt ctttaccggc aacaaccgcg gcgcatcgcg
     1501 ctgccgacct atcccttcgc gccggatcgc tactggattc cgacggtcgc tcccgaagtc
     1561 gatgtcgcgc gcctgctgga cgaagtgctc gatgaagtcg tcagcgtcga tgccgcgatc
     1621 gatgccttgc ggcgagccgg ttacggacag tcggaacaaa tctaggagaa cggggtggac
     1681 cgtctgcagg cgattttgca cgatttgaaa agtggtcgga tcgacaaggc cgaagctctt
     1741 cgccggctcg aagccggacg cgctcggccc gccgcggccg tctccctgtt ggccatggat
     1801 tggcgcgaac agcgcctggg tacgccatgt ggcgaaaacc ggcagcgcct gctgtgggcg
     1861 tgcgagcagt tgggaccggt tgacgcggcg acaccgctgt gcgccgggca ggctgatgcg
     1921 gtcgttcgct tcgagaccta cgctgcgcag ttgctggcgg cgctcaagga cttgcccgtg
     1981 catggacccg aggttctcgt gcaggttgcc gttccggcgg cgcaggaaac gctgttcggc
     2041 ctgtccggcc tgcttctgac ggcgcagttg gagaatccgc gcttgcgcgg ccaggtgatc
     2101 ggcctggaag gcgtagaaga catggccacc gtgttgcgtg acgagggcgg gcaagccgat
     2161 gctctggtgc gctatcgagc cggcgtgcgc tcggtgatgg tcagcgagga gtgcgccttg
     2221 gcgccggccg ggcatccctg gcgggacggc ggggtctatc tgattaccgg cggcgccggc
     2281 gggctgggca tgctgtttgc gcgcgacatc ctgcgccacg ccgggcaggc cacggtgatc
     2341 ctcgcgggtc gttcgtcgca gccggcgcag cggttgccgg cgtccctgca ctatcgccag
     2401 atcgatgtgg ccgacgccac ggcggtgagt gccggcgtac gcgcgatccg ggaagaattc
     2461 ggcactctga acggcatcct gcactgcgcc ggggtgctgc gtgacggcta cctgcggcac
     2521 a
//
LOCUS       DAOSIT010000345         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_35950_length_2513_cov_9.975590, whole genome shotgun sequence.
ACCESSION   DAOSIT010000345 DAOSIT010000000
VERSION     DAOSIT010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35950_length_2513_cov_9.975590"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="PLL14_08500"
     CDS             complement(<1..95)
                     /locus_tag="PLL14_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG04009.1"
                     /translation="MHSQRKLVQTSLIPIRWGDMDAYGHVNNTIY"
     gene            complement(208..2001)
                     /locus_tag="PLL14_08505"
     CDS             complement(208..2001)
                     /locus_tag="PLL14_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOG04010.1"
                     /translation="MSEYIAPIRDMQFVLKELAGLDEVAQLPGCEEATPDLVDAVLEE
                     AGRFAEEVLSPINWSGDQEGARWQDKAVTMPAGFREAYKLFADSGWTALACEPEWGGQ
                     GLPKVVAAAVAEMWKAANHSFSLCPLLTAGAIESLVLTGSDELKQTYLEKMVSGVWTG
                     TMNLTEPSAGSDLAAVRTRAEPQEDGTYKIFGQKIFITYGDHDMAENIIHLVLARTPT
                     APEGVKGISLFIVPKFMVNDDGSLGARNDAYCVSIEHKLGIHASPTAIMAFGDQSGAV
                     GYLVGEENRGLEYMFIMMNAARFGVGLEGVAACERAYQRARDYARERIQCTDIGVRGG
                     PKVAIINHPDVRRMLMTMKSRAEATRALAYVVAAAHDAAVHHPDNEVRKRNQAFVDLM
                     IPVVKGWSTESGVTMASLGVQVHGGMGYVEETGAAQHLRDAQISTIYEGTTGIQANDL
                     IGRKIAREGGVTLNALIADMRALDADLAQRSGGDLAAIRSRFAAGVDALASAGEWLVA
                     TYGKDVRAASAGAVPFLTLLGIVAGGWQMVRAALIAQKKIDAGDSDPFYPAKIVTTRF
                     YADHVLSQAAGLASSVTDGAEGVLALPEDMF"
     gene            complement(2014..>2513)
                     /locus_tag="PLL14_08510"
     CDS             complement(2014..>2513)
                     /locus_tag="PLL14_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04011.1"
                     /translation="SGAGPALELAEIMLSFGRVSGAAKTLEEYLAALPQESVRPWIRL
                     LQIYQRNGMRDEFEALTLKLNRNFNVEIMRWDGGDSRKELQLLPLDTQQAKAMTLEDI
                     PRIRDQIIALWGRPECPGYLEKLLRDNRDGQRSGFTLPVVEEILFLIDIVATREAAQQ
                     FSQNP"
BASE COUNT          477 a          807 c          736 g          493 t
ORIGIN      
        1 aagtagatgg tgttattgac gtgtccgtag gcgtccatat cgccccagcg gatcggaatc
       61 aggctggtct ggacgagttt gcgttggctg tgcatgggca ttcctggtct gattacgcgg
      121 gttccgtctt gccggcagac ggcaagacgg aacccgcaga acatggtcag cggcggcccg
      181 cccgaaggga ccgccgctat ccgccgctca gaacatgtct tcgggcagtg ccagcacccc
      241 ttcggcgcca tcggtcacac tgctcgccag accggcggcc tgcgacaaca cgtggtcggc
      301 atagaagcgc gtggtgacga tctttgccgg gtagaaggga tcactgtcac ccgcgtcgat
      361 ctttttctgt gcaatcagcg cagcgcggac catctgccaa cctccggcaa cgatgccaag
      421 cagcgtcagg aagggaaccg cgcccgccga tgcggcacga acatccttcc cataagtggc
      481 caccagccac tctccggcac tcgccagggc gtctacgccg gcggcaaagc gactacggat
      541 ggccgcgaga tctccaccac tccgctgagc cagatcggcg tcgagcgcac gcatgtcagc
      601 aatcagcgcg ttgagggtga cgccgccttc gcgcgcaatc ttgcggccga tcaggtcatt
      661 ggcctggata cccgtggtcc cctcgtagat cgtcgaaatc tgggcatcac gcaggtgctg
      721 cgcggcgccg gtttcttcga cgtaacccat tccgccatgt acctgcaccc cgagcgaagc
      781 catggtgact cccgattcgg tgctccagcc cttgaccacc gggatcatca gatcgacaaa
      841 ggcctggtta cgcttgcgta cctcgttgtc cggatggtgc accgcagcat cgtgcgcggc
      901 ggccaccacg taggccaggg cccgggtggc ctcggcacgc gacttcatgg tcatcagcat
      961 gcggcgaaca tcgggatggt tgatgatcgc cactttcgga ccgccgcgta cgccgatatc
     1021 ggtacactgg atgcgttcgc gcgcatagtc gcgcgcgcgc tggtaagccc gctcgcaggc
     1081 ggcaacgcct tcgaggccga cgccaaaacg cgccgcattc atcatgatga acatgtattc
     1141 caggccacga ttctcctcgc cgaccaggta gccgacggca ccgctttgat cgccaaaagc
     1201 catgatcgct gtcgggctgg cgtggatacc cagcttgtgc tcgatcgaca cgcaataggc
     1261 atcgttgcga gcccccaggc tgccgtcgtc gttgaccatg aacttcggaa caataaacag
     1321 cgaaatcccc ttgactccct cgggagctgt cggagtgcga gcaagcacga gatggatgat
     1381 gttctccgcc atatcgtggt caccataggt gatgaagatt ttctgtccga agatcttgta
     1441 ggtgccatct tcctgcggct cggcacgcgt gcgtacggcg gcaagatcgg agccggcgct
     1501 cggctcggta aggttcatcg tcccggtcca cacgccgctg accatctttt cgaggtaagt
     1561 ctgcttgagc tcgtcgctgc cggtcagcac cagcgactcg atggccccgg cggtcagcag
     1621 ggggcataga gaaaacgagt ggttggccgc tttccacatt tcggcgacgg cagcggcgac
     1681 gaccttgggc agcccctgac caccccactc gggttcgcag gccagcgccg tccagccgct
     1741 gtcggcgaac agcttgtagg cttccctgaa tccggcaggc atggtcaccg ccttgtcttg
     1801 ccagcgtgcc ccttcctgat caccggacca gttgatcggt gacaggactt cctcggcaaa
     1861 gcgacccgct tcttcgagaa ccgcatcgac cagatcaggg gtcgcttcct cgcaaccagg
     1921 caattgcgcc acctcgtcca gcccggcaag ttccttgagc acaaactgca tgtcacggat
     1981 gggggcaatg tattcactca tgtatttctc ctcttaaggg ttctgtgaaa actgctgcgc
     2041 ggcttcgcgg gtcgccacga tgtcgatcaa gaacaagatt tcctcgacta ccggcaaggt
     2101 aaaaccgctg cgctgtccat cgcgattgtc gcgcagcagt ttttccaggt aaccaggaca
     2161 ttccggcctt ccccagagcg caatgatctg gtcgcgaatg cgcgggatgt cctccagcgt
     2221 catcgccttg gcctgctgcg tatccagcgg caagagttgc agttctttac gcgaatcgcc
     2281 accatcccaa cgcatgatct caacgttgaa attccggttc agcttcagag tcagcgcttc
     2341 gaattcgtcg cgcatgccgt tgcgctggta aatctgcagc aggcgaatcc agggtcgcac
     2401 cgactcctgc ggcagggcgg cgaggtattc ctcgagagtt ttcgccgcac cactgacgcg
     2461 gccgaaagaa agcatgatct cggcaagttc gagcgccgga cccgcaccgc tcg
//
LOCUS       DAOSIT010000346         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36180_length_2501_cov_10.520850, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000346 DAOSIT010000000
VERSION     DAOSIT010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36180_length_2501_cov_10.520850"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="cas1c"
                     /locus_tag="PLL14_08515"
     CDS             complement(<1..930)
                     /gene="cas1c"
                     /locus_tag="PLL14_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOG04012.1"
                     /translation="MQLLNTLYVTLPESYLHLDNDTLRVEIERETRLRVPLHHLSAVV
                     CFGHVMVSPILMHRLADEGKSLVLLNAHGRFKARLEGPVSGNVLLRQAQHRRLESADY
                     CLSVAKSCIAGKLKNCRQVLQRGARENGREEDGAMLTASADELGRALSRLEHAADLDA
                     VRGIEGDQAKRYFAALSLLVRPEARPVFTMNGRTRRPPLDRMNALLSFLYAMLMNDAR
                     SALESVGLDPQIGFLHALRPGRAALALDLMEEFRPLLADRLALTLINRRQINADDFDE
                     HAGGAVTLKDKARRAVITAYQERKQEALTHPLLE"
     gene            complement(943..1572)
                     /gene="cas4"
                     /locus_tag="PLL14_08520"
     CDS             complement(943..1572)
                     /gene="cas4"
                     /locus_tag="PLL14_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOG04013.1"
                     /translation="MDAPDPILLSALQHWCYCPRQCALIHVEQVFADNLHTLRGQAVH
                     QQVDQPGVETRAGVRVERALPVWSDRLGLIGKADIVEFEADGTPYPVEYKHGSRSKAP
                     WIAACDDLQLAAQALCLEEMSGKPVPRGALYYATSRRRREVAITTALRQRVAEAAAAV
                     RGLIAAGSVPPAANDARCKNCSLLELCQPSLADSGALGAARARLFDADA"
     gene            complement(1559..1942)
                     /locus_tag="PLL14_08525"
     CDS             complement(1559..1942)
                     /locus_tag="PLL14_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOG04014.1"
                     /translation="MTAPWLLNENFPQPSVRRLRALGWDVVAISEESPSVDDEGVMER
                     ARTEGRWLATFDRDYGELVFKQQLPAPPVIVLLRVTSYLPEHPADWLVELQAGQQFQE
                     GCFHIFDGQTIRRRPFLAGLSGGRP"
     gene            complement(1939..2178)
                     /locus_tag="PLL14_08530"
     CDS             complement(1939..2178)
                     /locus_tag="PLL14_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOG04015.1"
                     /translation="MNWKDRITVDPQVLVGKPIIRGTRISVELLMDRLADGWSMEQIL
                     DSYPRVTREDVLAAIAFVTEVFREEDFVAIQKATV"
     gene            complement(2199..>2501)
                     /locus_tag="PLL14_08535"
     CDS             complement(2199..>2501)
                     /locus_tag="PLL14_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOG04016.1"
                     /translation="VDQGIYVCFGSMNPQLAERTGFSDADAETLKAIMPRLFENDASS
                     ARPEGSMAVKKVIWWKHGSKAGQCSSAKVHGSLKVDADGVITLADTCVEAEIIEGF"
BASE COUNT          445 a          779 c          830 g          447 t
ORIGIN      
        1 ttccagcagc gggtgcgtca gggcttcctg cttccgctcc tgataggccg tgatcacggc
       61 gcgccgcgct ttgtccttga gcgttacggc gccgcccgca tgctcgtcga aatcgtcggc
      121 gttgatctgg cggcgattga tcagcgtcag cgccaggcgg tcggccagca gcggccggaa
      181 ctcctccatc aagtcgagcg ccagcgcggc acgcccggga cgcagggcgt gaaggaagcc
      241 gatctgcgga tcgaggccga cactttccag cgccgagcgg gcgtcgttca tcaacatcgc
      301 gtagaggaag gagagcagcg cgttcatgcg gtcgagcggt ggccggcggg tgcggccgtt
      361 catggtgaaa accggccggg cttcaggacg caccagcagg gacagagcgg cgaaatagcg
      421 cttggcctgg tcgccttcga tgccgcgcac ggcatcgaga tcggcggcgt gttcgagtcg
      481 ggacaaggca cggccgagtt cgtcggcgct ggcggtgagc atcgcgccgt cttcttcacg
      541 accgttctcg cgagcgccgc gttgcagaac ctgacggcag ttcttcagtt tgccggcgat
      601 gcaggatttc gccaccgaca ggcaatagtc ggcgctttcg agacggcgat gctgggcctg
      661 gcgcagcaaa acgttgccgg aaaccgggcc ttccagccgt gccttgaagc ggccgtgagc
      721 attcagcagc accaggctct tgccttcatc ggcgagacgg tgcatgagga tcggcgagac
      781 catcacatgg ccaaaacaca cgacggcgga gagatgatgc agcggcacac gcaggcgcgt
      841 ttcgcgttcg atctcgacgc gcagggtgtc gttgtcgaga tgcaggtaac tttccggcaa
      901 ggtgacgtaa agcgtgttga gcagttgcat cgatttcccc gttcaggcgt cggcgtcgaa
      961 caagcgggcg cgggcggcgc ccagcgcgcc gctgtcggcc agggacggct ggcagagttc
     1021 gagcagcgag cagtttttgc agcgcgcgtc gttggcggcc ggtggcacgc tgccggcggc
     1081 gatcagcccg cgcacggcgg cggcggcctc ggccacgcgc tgacgcaagg cggtagtgat
     1141 ggccacttcg cggcgccggc gggaggtggc gtagtaaagg gcgccgcgcg gtaccggctt
     1201 gccgctcatt tcttccaggc acagcgcctg cgcggcgagt tgcagatcgt cgcaggcggc
     1261 gatccagggc gccttgctcc ggctgccgtg cttgtattcg acaggatagg gcgtgccgtc
     1321 ggcctcgaac tcgacgatgt cggccttgcc gatcaggcca aggcgatcgc tccacaccgg
     1381 cagcgcccgt tccacgcgca cgccggcgcg cgtctcgacg ccgggctggt cgacctgctg
     1441 atgcaccgcc tgtccgcgca gggtatgcag gttgtcggcg aacacctgct cgacgtggat
     1501 cagcgcgcac tggcgcggac agtagcacca gtgctggagg gcggagagga ggatggggtc
     1561 aggggcgtcc accggacagt cccgccagga agggacggcg acgtatcgtc tggccgtcga
     1621 aaatgtggaa gcacccttcc tgaaactgct gccctgcctg caactcgacc aaccagtcgg
     1681 ccgggtgctc cggcaggtaa ctggtcacgc gcagcaagac aatgactggc ggtgcaggca
     1741 gttgttgttt gaacaccagt tcgccatagt cgcggtcgaa ggtcgccagc cagcgacctt
     1801 cggtgcgcgc acgttccata accccttcgt cgtcgacgct tggactctct tcgctgattg
     1861 cgacgacatc ccagcccagg gcacgcagcc ggcgaaccga tggctgtggg aagttctcgt
     1921 tcaacagcca cggcgccgtc ataccgtggc cttctggatg gcgacgaaat cctcctcacg
     1981 gaaaacttcg gtaacgaaag caatcgccgc gagcacgtct tcacgcgtaa cgcgcgggta
     2041 tgaatcgaga atctgctcca tgctccagcc gtcggccaga cgatccatca gcagttcgac
     2101 ggaaatccga gtgccgcgaa tgatgggctt gccaaccaga acttgggggt cgacggtaat
     2161 gcggtctttc cagttcatgc cgagtgcctc cgtatctgtc aaaagccttc aatgatctcc
     2221 gcttccacgc aagtatccgc caatgtgatg accccgtcag catcgacttt cagactgccg
     2281 tgcaccttgg ccgaggagca ttgtccggcc ttgctgccat gcttccacca gatgactttc
     2341 ttcacggcca tgctgccttc cggacgcgcc gaagacgcat cattttcgaa caggcgaggc
     2401 ataatggctt tcagggtctc ggcatcggca tcggaaaaac cggtgcgctc ggccagttgc
     2461 gggttcatgc tgccgaagca gacgtagatg ccctgatcga c
//
LOCUS       DAOSIT010000347         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36222_length_2499_cov_11.854746, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000347 DAOSIT010000000
VERSION     DAOSIT010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36222_length_2499_cov_11.854746"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLL14_08540"
     CDS             <1..723
                     /locus_tag="PLL14_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG04017.1"
                     /translation="ASIEELTVSINEVAQNARATGDSSTAAANTAQQGQVLVHASADE
                     IEKVSEIVARSSAQIRQLANRSREIGGIADVIKEIAEQTNLLALNAAIEAARAGEQGR
                     GFAVVADEVRKLAERTSKATGEIGSMVVSVQADTDTAVLAMEEAMPQVTRSLERAREA
                     GSLLAASHHQALESSERVQQVAMATREQASVADDIARHVQHIASMTEETSTTVRSNAA
                     AAAELDRLAGELRAAVGYFRVA"
     gene            complement(844..>2499)
                     /gene="recG"
                     /locus_tag="PLL14_08545"
     CDS             complement(844..>2499)
                     /gene="recG"
                     /locus_tag="PLL14_08545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG04018.1"
                     /translation="RVVADDEPLPQSLTPLYPTTAGLTQTVLRRLIMHALRQVDLAEV
                     LEESWCCGQALPSFAAAVSFLHAPPPSVSVAALENRTHPAWQRIKFDELLAQQLSLRR
                     AYLARRHLGAPALPAACPLARRLEAALPFTLTAAQQRVAAEIAADLEQPRPMQRLLQG
                     DVGCGKTVVAALAACQAIDAGFQAALMAPTEILAEQHFLKLRAWLEPLGVEVAWLAGG
                     LGKTLRRAKQQQAAKTAQLVVGTHALIQESIDFARLGLAIVDEQHRFGVVQRLELRRK
                     GSNPHQLMMSATPIPRTLAMSYYADLDVSVIDELPPGRSPVRTRLFSDTRRDDVIAAV
                     RAVVAGGRQAYWVCPLVEESAKLELQAALVTHTSLSVELSGLRVGLVHGRLRANDKGA
                     VMAAFAAGEIDVLVATTVIEVGVDVANATLMVIEHAERFGLAQLHQLRGRVGRGTHDS
                     VCVLLYQQPLSATARARLKIIFEHTDGFEIASQDLRLRGPGEFVGSRQSGVPLLRYAD
                     LALDGELVELARAMAATLLREAPERAARHLQRWLGNREELLKA"
BASE COUNT          464 a          846 c          827 g          362 t
ORIGIN      
        1 gcttcgatcg aggaactgac ggtgagcatc aacgaggtcg cgcagaacgc gcgcgctacc
       61 ggcgacagtt ccaccgccgc agcgaacact gcccagcagg gccaggttct ggtgcacgcg
      121 tcggccgacg agatcgagaa ggtttccgag atcgtcgctc gatcgtcggc acagatccgg
      181 caattggcca accgctcccg agaaattggc ggaattgccg acgtcatcaa ggaaatcgcc
      241 gaacagacca atctgctggc gctgaatgcg gccatcgaag cggcacgcgc cggcgagcag
      301 ggtcgcggtt tcgcggtcgt cgccgacgaa gtccgcaagc ttgccgagcg gaccagcaag
      361 gccacgggag aaatcgggtc aatggtggtc agcgttcaag ccgataccga tacggcagtc
      421 ctcgccatgg aagaggccat gccacaggtc accagaagtc ttgaacgggc gcgcgaagca
      481 ggcagcctgc tggcagccag ccaccaccag gcgctggagt cgagcgagcg cgtgcagcag
      541 gtggcgatgg ccacgcgcga acaggccagc gtcgccgacg atatcgccag gcatgttcag
      601 cacatcgcgt cgatgaccga ggaaaccagc acaacggtac gaagcaacgc cgccgccgcg
      661 gccgagcttg accgcctggc gggtgaactg cgcgcggccg tcggctattt ccgggtcgca
      721 tgaacgatgc gtgtagcctt gtggcatgca ggtgccgcaa ggccgacagc gctgcaccgt
      781 tgtttggaac ttcgtcgggc cagggtggcg ggctcggcgc cggaccacgg cctgtcctgg
      841 gcttcaggct ttcagcagtt cctcccgatt ccccagccag cgctgcaggt ggcgcgccgc
      901 gcgctcgggc gcctcgcgca acagtgtcgc cgccatcgcc ctggccagtt cgacgagttc
      961 gccatcgagc gcgaggtccg cgtagcgcag cagcggcact cccgactggc ggctgccgac
     1021 gaactctccc ggacctcgta aacgaagatc ctggctggca atctcgaatc cgtcggtgtg
     1081 ctcgaaaatg atcttcaggc gggcccgcgc ggtcgccgac agcggctgct ggtagagcag
     1141 cacacagacc gagtcatggg tgccgcgacc gacgcggccg cgcaactggt ggagctgcgc
     1201 caacccgaag cgctcggcgt gttcaatgac catcaaggtg gcgttggcca catcgacacc
     1261 gacttcgatg accgtcgtcg cgaccagtac gtcgatctcg ccggcagcga aggccgccat
     1321 cacggcaccc ttgtcgttgg cccgcagacg cccgtgcacc agcccgacgc gcagaccgga
     1381 cagctcgacg ctcaagctgg tgtgggtcac gagggcggcc tgcagttcca gtttggcgga
     1441 ctcctcgacc aggggacaaa cccagtaagc ctgccgcccg ccggcaacga cggcgcgcac
     1501 ggcggcaatc acatcgtctc gccgggtatc cgagaacagc cgggtgcgaa ccggcgatcg
     1561 gcccggcggc agttcatcga tcaccgaaac gtcgagatcg gcgtaatagc tcatcgccag
     1621 cgtgcgcgga atcggcgtcg ccgacatcat caactggtgc gggttgcttc ccttgcgccg
     1681 caactcgagc cgctgcacga cgccgaagcg atgctgttcg tcgacgatcg ccagcccgag
     1741 gcgcgcaaaa tcgatgctct cctggatcag cgcgtgcgtc ccgaccacca attgcgcggt
     1801 cttcgcggcc tgctgttgct tcgcccgcct gagcgtcttg ccgaggccgc ccgccaacca
     1861 cgccacctcg acgcccagtg gctccagcca ggcgcgcagt ttcaggaaat gttgttcggc
     1921 aaggatctcg gtaggcgcca tcaaagccgc ctgaaaaccc gcgtcgatcg cctgacacgc
     1981 cgccagggca gcgacgacgg tcttgccgca gccgacatcc ccctgcagca gacgctgcat
     2041 cggacgcggc tgttcgagat cggccgcgat ctcggcggcg acacgctgtt gcgccgcggt
     2101 cagcgtaaaa gggagagcgg cttcgaggcg ccgggcgagt gggcaggcgg caggcaaggc
     2161 cggtgcaccc agatgccggc gagccaggta ggcacggcgc agcgacagct gctgtgcgag
     2221 caactcgtcg aacttgatgc gctgccaggc cggatgcgtg cggttctcca gtgccgccac
     2281 ggaaacagac gggggcggcg cgtgcaggaa agacaccgcc gccgcaaatg acggcagcgc
     2341 ctgcccgcag caccagctct cttcgagcac ctcggcgaga tcgacctgcc gcagcgcgtg
     2401 catgatcagg cgacgcagta ccgtctgcgt caaccccgcc gtcgtcggat agagcggcgt
     2461 cagcgactgc ggcagcggct cgtcgtcggc aaccacccg
//
LOCUS       DAOSIT010000348         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36259_length_2497_cov_7.339885, whole genome shotgun sequence.
ACCESSION   DAOSIT010000348 DAOSIT010000000
VERSION     DAOSIT010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36259_length_2497_cov_7.339885"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2497)
                     /locus_tag="PLL14_08550"
     CDS             complement(<1..>2497)
                     /locus_tag="PLL14_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04019.1"
                     /translation="APIHDMQQRVLLATVILTLLAGSLTWWILSREFSPMLAAARTLS
                     AMSEAEQPLQPLPIIRQDEIGGLVEGFNHLLDTLGQRDTALRESEAGYRTLFREMLSG
                     FALHEILCDDEGKPVDYRFLAINPAFTRMTGLKAEDLVSRTVLEVMPGTERYWIEIYG
                     KVALTGEPAFFENYAAELQRYFEVSAFRPAPNQFACSFSDITERKRAEMLLRDEEQHF
                     RNLANGGSTLIWTSGPDKLCSYFNEPWLRFTGRSLEQELGNGWTEGVHPDDFSSCVQI
                     YATAFDRREPFSMQYRLRHADGSYRWIRDDGTPRYNSQGEFLGYIGFCVDITEQIRTA
                     DELDQYRQHLENLVQERTLELAQAKEAAEAANLAKSSFLANMSHEIRTPINAIIGLTH
                     LLRRAEPTPVQADRLGKIDAAAAHLLSLINDILDISKIEAGKLELEQTNFALNAVLDN
                     VRSLISDQVRAKGLVIEVDPDGVPLWLRGDPTRLRQALLNYISNAIKFTEQGLITLRT
                     ILVDDRRDRVLVRFEVQDSGIGIKADQLPSLFQAFEQADASTTRRYGGTGLGLAITRR
                     LAKLMGGDAGVESQPGKGSTFWFTACLGHGHGIMPVTTAGGVGDAEAELRKHHGGARL
                     LLAEDNAINREVALELLHGAGLAVDVAIDGQDALDKARAIAYPLILMDVQMPRMDGLQ
                     AARAIHSLPGQAATPILAMTANVFDEDRRACLEAGLNDFVAKPVDPKTLYAALLKWLP
                     MLSSVAPASVLAPLPEPDRWRRQLTAIPGLDIERGLALVRGNATTYLRLLNLFIDTHV
                     QDPGHFGEDLAAGDLASIKARAHALKGSAGNIGA"
BASE COUNT          482 a          772 c          757 g          486 t
ORIGIN      
        1 cggcaccgat gtttccggca gaacctttca aggcgtgcgc ccgcgccttg atcgacgcca
       61 ggtccccagc cgccagatcc tcgccgaaat gccccggatc ctgaacgtgc gtgtcgatga
      121 acaggttcag caaccgtaga tatgttgtcg cattgccgcg caccagggcc agcccgcgct
      181 cgatgtccag acctggaata gccgtcagtt gccgccgcca gcgatccggt tccggaagtg
      241 gcgccagcac gctcgccggg gccactgagg acagcatcgg cagccacttg agcagtgcgg
      301 catagagggt cttcggatca accggcttgg cgacaaagtc gttcaaaccg gcttccaggc
      361 aggctcgccg atcctcgtcg aagacgttgg cggtcatcgc caggattggc gtggccgcct
      421 ggccaggcag tgagtgaatg gcacgcgccg cctgcagtcc gtccatgcgc ggcatctgca
      481 catccatcag gatcaacggg taggcgatgg cgcgcgcctt gtcgagcgca tcctgaccat
      541 cgatggccac atcgacggcg agtcccgctc cgtgcagcag ttccagggca acctcgcggt
      601 tgatggcatt gtcttcggcc agcagcagtc gtgcaccacc atggtgcttg cgcaactcgg
      661 cttcggcatc gcccactccg ccagcagtag tgaccggcat gatcccgtgg ccgtgcccaa
      721 ggcaggccgt gaaccagaaa gtgctgcctt tccccggttg gctctcgacg ccagcgtcgc
      781 cgcccatgag tttcgccaac cgccgggtga tcgccagccc gaggccggtg ccgccgtacc
      841 tgcgagtggt cgaggcgtcg gcctgctcga acgcctggaa cagactgggc agttgatccg
      901 ctttgatgcc gatgccggaa tcctgaactt cgaagcgcac cagtacccga tcacggcgat
      961 cgtcaaccag gatggtacgc agggtgatca aaccttgctc ggtgaacttg atggcattgc
     1021 tgatgtaatt gagcagcgcc tgacgcaacc gcgtaggatc gccacgcagc cacaggggca
     1081 cgccgtcggg atcaacctcg atgaccaggc ccttggcgcg cacctggtcg gagatcagcg
     1141 agcggacgtt atccaggacg gcattcagcg cgaagttcgt ttgctctagt tccagtttcc
     1201 cggcctcgat cttggagata tcgaggatgt cgttgatcag ggacaggagg tgagccgccg
     1261 ccgcatcgat cttgcccaac cggtcggcct gtacaggtgt tggttctgct cggcgaagca
     1321 gatgcgtgag gccgatgatg gcattgatcg gagtgcggat ttcgtgactc atattcgcca
     1381 ggaagctgct cttggccaga ttggcagctt cggcggcctc cttggcctgc gccagttcca
     1441 gcgtccgctc ctggacgagg ttctccagat gctggcgata ctggtccagt tcgtcagcgg
     1501 tccggatctg ctcggtgata tcgacacaga agccaatata gccgaggaat tcaccctgac
     1561 tgttatagcg gggggtgccg tcgtccctta tccatcgata gctgccgtcg gcatggcgca
     1621 ggcgatattg catgctgaag ggttcacgcc ggtcaaaggc tgtggcgtag atttgcacgc
     1681 aactggaaaa atcgtccgga tgcacaccct ccgtccatcc attgccaagt tcctgttcca
     1741 gagagcgtcc ggtaaacctc aaccagggtt cgttgaagta gctacagagc ttgtccgggc
     1801 cagaggtcca gatcagtgtc gacccaccat tcgcgagatt ccggaagtgc tgctcctcgt
     1861 cgcgcaacag catctcggcg cgcttgcgct ccgtgatatc ggagaaggaa caggcaaact
     1921 ggttgggagc tggccggaag gcggatacct cgaagtatcg ctgcagttcg gcggcgtagt
     1981 tctcgaaaaa cgccggctcg ccagtgaggg ccactttgcc ataaatctcg atccagtatc
     2041 tctcggtccc cggcatgacc tccagaacgg tccgactcac gaggtcttcc gccttgaggc
     2101 cggtcatgcg ggtgaaagcc ggattgatgg ccagaaagcg gtagtcgacg ggcttgccct
     2161 cgtcgtcaca aagaatttcg tgcagcgcaa aaccactcaa catttcgcgg aacagcgtcc
     2221 gatagccggc ctcgctctcc cgcaaagccg tatctcgctg ccccaaagtg tcaagcagat
     2281 gattgaatcc ctcgaccagt ccaccaattt cgtcctgtcg gatgatgggc aggggctgca
     2341 ggggttgctc tgcctccgac atggcggaca atgttctcgc agcagcaagc atcggcgaga
     2401 actctcgact caatatccac caggtcaggc tgcccgccag cagcgtcagg atgaccgtgg
     2461 ccagcagcac acgctgctgc atgtcatgga tcggggc
//
LOCUS       DAOSIT010000349         2485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36487_length_2485_cov_6.694650, whole genome shotgun sequence.
ACCESSION   DAOSIT010000349 DAOSIT010000000
VERSION     DAOSIT010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36487_length_2485_cov_6.694650"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="PLL14_08555"
     CDS             <1..168
                     /locus_tag="PLL14_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOG04020.1"
                     /translation="MSVVTLRPEVGFSGGRLPTREEVLQLHHLAHTECFIANSVRTEV
                     RCEPVFGSSEA"
     gene            complement(417..800)
                     /locus_tag="PLL14_08560"
     CDS             complement(417..800)
                     /locus_tag="PLL14_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG04021.1"
                     /translation="MDKPGNPVGWFEIYVSDMDRARTFYTAVFGRELTSLPEIGEGGE
                     MYAFPWVEGGTGAAGALVKHPMACSGKGGTMVYFSCDDVSNEANRAVEAGGRIMQPKM
                     AIGQYGHIALVEDTEGNLIGLHSMQ"
     gene            complement(865..1524)
                     /locus_tag="PLL14_08565"
     CDS             complement(865..1524)
                     /locus_tag="PLL14_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04022.1"
                     /translation="MALDAFQDIFCRMIASSDYRARLIDNPEAEFDQHDLDAREKRRL
                     TAMASHSGMRVNTAIHRANRLTPLDQTLPFTCFLLGSRLGALLDRYWHSNPTENLQLP
                     AECVRFADFIVDELQSGRLVDPYIGEVLAFERACTELRFFSAEELASRSGTQARLPAK
                     VRVLHFQHDPEPLLAALSELKSPPDSLAEGDFHLAVDARSGEVVFRLLDPVVAAALLG
                     S"
     gene            complement(1532..2428)
                     /locus_tag="PLL14_08570"
     CDS             complement(1532..2428)
                     /locus_tag="PLL14_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HOG04023.1"
                     /translation="MFSEQKLGIGLLFNPALPQFLETRPESFDYLEVIPDREWADRGV
                     HGSPRFLLLEQSLEFLERSRERKPMLCHSIGLSIGSAAIFDSGHVEQIRWLHQKLDFA
                     WHSDHLSFSRLPQDLAAAGNGPEMHAAISLPVPYDQQVLQLIAERVRYIRAVIDCQFL
                     LENNVYYAAIPEEEMSEAEFLNRLCQVSGCGILLDLHNLYVNSRNHGISMEAFLRDID
                     LRHVVEIHLAGGDELLGFYTDSHAGPVAEPVWEMLDETLRAAPWIRAVTFEFHESSYP
                     LLKTAGIAAQLARARRIWERHC"
BASE COUNT          509 a          746 c          763 g          467 t
ORIGIN      
        1 atgagtgtcg tcacgctcag gcccgaagtc ggtttctcgg ggggtcgcct gccgacccgc
       61 gaggaagtcc tgcagcttca tcatctggcg cacacggagt gcttcatcgc caattcggtc
      121 aggaccgagg ttcgctgtga accggttttc gggtcgtcgg aagcctgagc cccagccgtg
      181 gcgaaggcac gagaccgagg tcgggatgcg gcctcgaaag tttcgcgggc atacagcgtc
      241 gcagacgaaa ggcgatcgtt ccgccccctg gcggtctgaa acgcgataca cgcacgccac
      301 ggcggatgac aaggggccgg gatggcacgg gcaacgcgcc acgcggctcc cggctcacta
      361 cggctacggc aggcagtcag ctcgatccgg ccctgtccgg cgtccgggga cggcgactac
      421 tgcatggaat gcaggccgat caaattgccc tcggtgtctt ctaccagcgc aatatggccg
      481 tattgcccaa tcgccatctt tggttgcatg atcctgccac cggcctcgac cgcccgattc
      541 gcttcgttgc tgacatcatc gcaggagaaa tacaccatcg tcccgccctt cccggagcaa
      601 gccatcggat gcttgacgag cgcaccggct gcaccggtcc cgccctcgac ccagggaaag
      661 gcgtacattt cgccaccttc accgatctcg ggtagcgagg tcaactcgcg gccgaagact
      721 gcagtgtaga aggttctcgc gcgatccatg tcggaaacgt agatctcgaa ccacccaacc
      781 gggttacctg gcttgtccat gttcatgctc cttggtttgt tgtgggaaac gcatcgtgaa
      841 atattggctg cgaatttcga gatatcagga gcccagcaag gccgccgcca cgacgggatc
      901 gagcaacctg aataccacct cgccggaacg cgcgtcgacc gcgagatgga aatcgccttc
      961 ggcaaggctg tccggcggcg atttcagttc actcaaggct gcgagcaatg gctccggatc
     1021 atgttggaaa tgcagtacac gcactttggc tggcagtcgc gcctgcgtcc ctgatctcga
     1081 agcgagttcc tcggcgctga agaaacgaag ttcggtgcac gccctttcga aagccagaac
     1141 ttcgccgatg taagggtcga ccagccgtcc actctgcagt tcgtcgacga tgaagtccgc
     1201 gaaacgcacg cactcggcgg gcaactgcag gttttcggtg gggttgctgt gccagtagcg
     1261 gtccaacaat gcccccagtc gtgaaccgag cagaaagcag gtgaatggga gcgtctgatc
     1321 cagcggtgtc aagcgattgg cgcgatgaat cgccgtgttg acccgcatac cggaatgcga
     1381 cgccatcgcc gtcagacgac gcttctcgcg tgcatcgagg tcgtgttgat cgaactcggc
     1441 ttcggggttg tcgatcaagc gtgcacggta gtccgacgag gcgatcatcc ggcagaagat
     1501 gtcctgaaag gcgtcgaggg ccatgttcaa ctcagcaatg ccgttcccag atccggcgcg
     1561 cgcgcgccaa ttgcgcggca atgcctgcgg tcttgagcaa gggatacgaa gactcatgga
     1621 actcgaaggt cacggcgcgg atccaggggg ccgcccgaag cgtctcgtcc agcatttccc
     1681 aaaccggctc ggcaacgggt cccgcgtgcg aatcggtgta aaaaccgagc aactcgtcgc
     1741 cacctgcgag gtgaatctcc acgacgtgac gcaggtcgat atcgcgcaga aaagcctcca
     1801 tggaaatccc gtgattccgc gaattgacgt agaggttgtg cagatcgagc aggatcccgc
     1861 agccggaaac ctggcacagg cggttgagga actcggcttc gctcatctcc tcttccggta
     1921 ttgcggcgta gtagacgttg ttctcgagca ggaactggca gtcgatcacg gcgcggatat
     1981 acctgacgcg ctcggcgatg agttgcaaaa cctgttgatc gtaaggtacc ggcagcgaga
     2041 tggcggcgtg catttcgggg ccgttgcctg ccgcggccag gtcctgcggc aagcgggaga
     2101 acgaaagatg gtcgctgtgc cacgcgaagt ccagcttctg gtgcaaccag cgaatctgtt
     2161 cgacatggcc gctatcgaaa attgccgcac tgccaatcga caggccgatc gaatgacaaa
     2221 gcatgggctt ccgttcgcgg ctgcgctcga gaaattcgag cgactgttcg aggagcagga
     2281 accgcggcga gccatgcacg ccgcggtccg cccattcccg atcggggatt acctccagat
     2341 agtcaaacga ctccggtcgg gtttcgagaa actgcggcag cgccgggttg aacagcaggc
     2401 cgatgccgag cttctgttct gaaaacacac atcagccctg ctggacgacc tgttcgaatt
     2461 tctgcttgat gttcaggtcc aggcc
//
LOCUS       DAOSIT010000350         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36659_length_2476_cov_9.506402, whole genome shotgun sequence.
ACCESSION   DAOSIT010000350 DAOSIT010000000
VERSION     DAOSIT010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36659_length_2476_cov_9.506402"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLL14_08575"
     CDS             complement(<1..269)
                     /locus_tag="PLL14_08575"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HOG04024.1"
                     /translation="MNAPETRPTPATMADVQGSADTRQIAINKVGIKAIRHPVKVLDK
                     SGGIQHTIAMFNMYVGLPHNFKGTHMSRFVEILNSHEREISVENFP"
     gene            complement(285..2180)
                     /gene="dxs"
                     /locus_tag="PLL14_08580"
     CDS             complement(285..2180)
                     /gene="dxs"
                     /locus_tag="PLL14_08580"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG04025.1"
                     /translation="MTRYPLLDTISEPAQVRKLDRRQLPQLADELRAFLLESVARTGG
                     HLSSNLGTVELTVALHHVFDTPHDRLVWDVGHQTYAHKVLTGRRSGMGKLRMRGGVSG
                     FPKRSESSYDTFGVGHSSTSISAALGMALAAKIKGETRKVVAIIGDGAMSAGQAFEAL
                     NNAGVADADMLVVLNDNDMSISPAVGALNRYLARLFSGSSFNAARKAGEKILDFSPSL
                     REFAKRAEEHVKGMLTPGTLFEEMGFNYIGPIDGHDLESLVPTLQNLKNLKGPQFLHV
                     ITKKGQGYKLAEVDPILYHGVSKFKPDVGIADGPSGAKPSYTQIFGDWLCDMAAADPK
                     LVGITPAMREGSGLLRFSERYPERYYDVGIAEQHAVTFAAGLACEGLRPVLAIYSTFL
                     QRGYDQLVHDVALQNLPVVFALDRGGLVGADGPTHHGAYDLSFLTCIPNLVVMTPSDE
                     DECRKMLTTAYRLDGPAAVRYPRGTGSGTSIDKKLVGLPLGKGEIRRRGHGVALLAFG
                     SMLTPALAAAEELDATVANMRFVKPIDRELITALAAEHSLLISVEENALIGGAGAEVA
                     RVLEDTGNPVRLLRLGIPDRFIEHGDQALLLAEIGLDRDGIVRAVRQSQDSAADSCTP
                     AGPTSGE"
     gene            complement(2229..>2476)
                     /locus_tag="PLL14_08585"
     CDS             complement(2229..>2476)
                     /locus_tag="PLL14_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG04026.1"
                     /translation="GLLFQVVDDILDCTASTATLGKTAGKDAASEKPTYVSLMGLERA
                     RELAADLRAQALAALTVFGERSQRLMELTDFITQRKF"
BASE COUNT          447 a          726 c          806 g          497 t
ORIGIN      
        1 gggaaattct cgaccgaaat ctcgcgctcg tggctattga gaatctccac gaagcgcgac
       61 atgtgggtgc ccttgaagtt gtgcggcagg ccgacgtaca tgttgaacat ggcgatcgtg
      121 tgttgtatcc cgccggattt gtccaggacc ttgaccggat gacggatcgc cttgatcccc
      181 accttgttga tggcgatctg tcgggtgtcg gccgagccct ggacgtccgc catggtggcg
      241 ggtgtgggtc gcgtttcggg ggcgttcatt gctgctcctg gtaattattc tccgctggtg
      301 ggacctgctg gtgtgcagga atccgcagcg ctgtcctggc tctggcgtac cgcccggact
      361 atgccgtccc ggtccaggcc gatttcggcc agcagcagtg cctggtcgcc atgctcgatg
      421 aagcggtcgg gaattcccag ccgcagtaag cgcaccgggt ttccggtgtc ttcaagaacg
      481 cgcgcaacct cggcacctgc gcctccgatc aaagcatttt cttcgacgct gatcagcagc
      541 gaatgttccg cagccagtgc cgtgatcaac tcgcggtcga tcggcttgac aaagcgcatg
      601 ttggctacag tcgcgtcgag ttcctcggcg gccgccaggg cgggggtcag catgctgccg
      661 aaggcgagca gcgccacgcc atggccacgg cgacggattt cgcccttgcc aaggggtagt
      721 ccgaccagtt tcttgtcaat ggacgttccc gaaccggtgc cacgcgggta acgcaccgcg
      781 gcagggccgt ccaggcggta ggcggtggtc agcatcttgc ggcactcgtc ctcgtcggat
      841 ggcgtcatca ccaccaggtt gggaatacag gtcaggaacg acaggtcata ggcgccgtgg
      901 tgggtcgggc cgtcggcacc taccaggcca ccgcggtcga gcgcgaagac cactggcagg
      961 ttctgcagag ccacatcatg caccagttga tcatagccgc gttgcaggaa ggtcgagtag
     1021 atcgccagga caggccgcag accttcgcag gcgagtccgg cggcgaaggt aacagcatgt
     1081 tgttcggcga taccgacgtc gtaatagcgt tccgggtagc gttcgctgaa gcgcagcagg
     1141 ccagagcctt cacgcatcgc cggggtgata ccgaccagtt tcgggtcggc cgccgccatg
     1201 tcgcatagcc agtcgccgaa gatctgggta taactgggtt tggcgccgct tggcccgtcg
     1261 gcgatgccga catccggctt gaacttcgac acgccatggt aaagaatcgg gtcgacttcg
     1321 gccagcttgt agccttggcc cttcttggtg atcacatgca gaaactgcgg ccccttcaga
     1381 ttcttcaggt tctgcagtgt gggaaccagc gattcgaggt cgtggccgtc gatcggaccg
     1441 atgtagttga agcccatttc ctcgaacagc gtaccgggtg tcagcatgcc tttgacgtgt
     1501 tcctcggcgc gtttggcgaa ttcccgcagc gaaggcgaga agtcgagtat cttctcgccc
     1561 gccttgcgcg cggcgttgaa actgctgccc gagaagaggc gcgccagata gcggttgaga
     1621 gcccccactg ccggtgaaat agacatgtca ttgtcgttga ggacgacgag catgtcggca
     1681 tccgcgacgc cggcattgtt gagggcctca aaagcctggc cggcggacat cgcgccatcg
     1741 ccgatgattg ccacgacctt gcgtgtctcg cccttgatct tggcggcgag ggccatgccc
     1801 agcgcggccg agatcgaggt cgacgaatga ccgacgccga aagtgtcgta gctgctctcg
     1861 ctgcgcttcg gaaaacccga aacgccacca cgcatgcgca gcttgcccat acccgagcga
     1921 cgaccggtga gtaccttgtg tgcgtaggtc tgatggccta cgtcccacac caggcgatcg
     1981 tgcggggtat cgaaaacgtg gtgcagggca accgtgagtt cgaccgtgcc caggttcgac
     2041 gagaggtgtc cgccggtcct ggcgaccgat tccaacagga aggcgcgcaa ctcatcggca
     2101 agttgtggca attgtcggcg gtcaagtttg cgtacttgcg ccggttcgct gatggtgtcg
     2161 agcagagggt aacgggtcat tgattttcct ggtcctgcgg aaacgctcgg cggcaagcag
     2221 agaagaggtc agaacttgcg ctgggtaatg aaatcggtca actccatcag gcgctggctg
     2281 cgttcgccga aaacggtgag ggccgcgagt gcttgtgctc gtagatcggc agcaagttcg
     2341 cgggcgcgct ccaggcccat caggctgacg taggtcggtt tctcggaagc cgcatccttg
     2401 ccggccgtct tgcccagtgt tgccgtgctg gccgtgcagt caagaatgtc atcgacgacc
     2461 tggaatagca gcccgg
//
LOCUS       DAOSIT010000351         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_36751_length_2470_cov_8.080331, whole genome shotgun sequence.
ACCESSION   DAOSIT010000351 DAOSIT010000000
VERSION     DAOSIT010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36751_length_2470_cov_8.080331"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLL14_08590"
     CDS             complement(<1..910)
                     /locus_tag="PLL14_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG04027.1"
                     /translation="MSDHHHDHDEHQHAGHEHGAQAHHHHADAGPRLVWALLLTLAFA
                     AVEALTGFWSGSLALLGDAGHMVTDSASLALAAFAARLAQRPPTRRHTYGYGRVETLA
                     ALLNVVFMVLVVVGISVAAVGRILEPAAVNGEAVTLVAALGLFLNIAVAWLLMHGEQT
                     MNTRGALLHVLGDLLGSVAALVSGAVVLWTGWTTADPLLSLLICALMLASSLRLLREV
                     LLALMEGVPPSLSTEHVGRLLAGVAGVASVHDLHIWTLSSRRIALSAHLIVESLEQWP
                     TILAASRHTLADQGITHVTLQPEPLRN"
     gene            1074..>2470
                     /locus_tag="PLL14_08595"
     CDS             1074..>2470
                     /locus_tag="PLL14_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOG04028.1"
                     /translation="MNPLPSRDSINRFFVLSGLWVTLHVSVFTTQQAYSTLAVALWTL
                     ATTVSYAFLYLLPALLPGYLLHGFLARRAASRGGVVALAILLIGLTGMVQVLLFADRV
                     IYGMYGFHINGFVLDLVFTPGGIASLGASRSTELTAALVVIALLALQAATYYWTTRRC
                     AGRLPLRWMPRASWLLLAILSFAVGERVAYGFSAAANYQPILFASERYPLYLPTTFRK
                     LASRLGVSVTSEAADGLHVKHSVLNYPLVPLRVEPPARPLNIVWLAVESLRYDMLDRT
                     IMPQLWDFSEHALRLEQHYSGGNTTQMGIFSMFYGLYGNYWFPMQAARRSPLLMDVLQ
                     QQNYQFSLHTSQSFTYPPFQETVFARMNPADMHALSEGPPPWQRDRQNIDDILKFIDS
                     RDRSRPFMTYMFFEGTHANYTFPPESVIARPYLDDFNYLSADFAQQMVPIKNRYINAA
                     HHVDQQIGRIIAHLRT"
BASE COUNT          449 a          809 c          733 g          479 t
ORIGIN      
        1 tgttgcgcag gggttccggc tgcagcgtca cgtgggtgat gccctgatca gcgagtgtgt
       61 gccgactggc ggcgagtatg gtcggccact gttcgagcga ttcgacgatc aggtgcgccg
      121 acaaggcaat ccgccgagag gacagggtcc agatatgcag gtcgtgcacc gaggcgacgc
      181 ctgcgacgcc ggccagcagg cgcccgacgt gctcggtcga caggctgggc ggcacgcctt
      241 ccatcagcgc cagcagaact tcgcgcaaca ggcgcagact ggaagccagc atcagtgcgc
      301 agatcaacag cgaaagcagc gggtcggccg tcgtccaacc ggtccatagc accaccgcgc
      361 cggacaccag cgcggcgacc gaccccagca gatcgccgag gacgtgcagc agggcgccgc
      421 gcgtattcat tgtctgctca ccgtgcatca gcagccaggc aacagcgatg ttcaggaaca
      481 gtccaagcgc cgccaccaga gtaaccgcct caccgttgac tgccgccggc tccagaatgc
      541 ggccgaccgc ggcgaccgag atgccgacca ctaccaggac catgaacacg acgttgagca
      601 gcgccgccag tgtctcgaca cggccatagc cgtaggtatg tcgacgtgtc ggcgggcgtt
      661 gcgccagcct tgcggcgaag gcggcgaggg ccagcgacgc gctgtcggtg accatatgcc
      721 cggcatcgcc gagcagtgcc agcgatcccg accagaagcc ggtcaacgcc tcgacggcgg
      781 caaaggcgag cgtcagcagc aaggcccaga ccagtcgtgg accggcatca gcgtggtgat
      841 gatgcgcctg cgcgccatgc tcgtggccgg catgctggtg ctcgtcgtga tcatgatgat
      901 ggtcgctcat gacaggtatt ctaaccgaca tcaatgactg ctcgtccgct tcatcagtta
      961 gcgcctcgac acggctcagc tggccgcgcc aacgccggtc gacggtgtgt gcacggctta
     1021 aggccaggct aatctttatc cgctaaaata tcggcttacc cccaaggatg ccgatgaacc
     1081 cattgccttc tcgcgacagt atcaaccgtt tcttcgttct ttccggactg tgggtgaccc
     1141 tgcacgtcag cgtgtttacc acccagcagg cctacagcac ccttgcggtg gcgctgtgga
     1201 cgctggcaac cacggtcagc tacgcgttcc tctacctgct gccggcactg ctccccggct
     1261 atctgctgca cggattcctg gcacggcgtg ccgcgagccg aggcggggtg gtcgcgttgg
     1321 cgattttgct gatcggtctg accgggatgg ttcaggtgct gctgttcgcc gaccgggtga
     1381 tctacggcat gtacggattt cacatcaacg gctttgtcct cgatctggtg ttcaccccgg
     1441 gcgggatcgc ttcgctgggg gcaagtcgca gcactgaact gactgccgcc ctggtggtca
     1501 tcgcccttct ggctctgcag gcggccacct actactggac gacgcggcgc tgcgcgggtc
     1561 ggcttccctt gcggtggatg ccgcgtgcga gctggttgct gctggcgatc ctgagttttg
     1621 cagttggcga gcgggtggcc tatggtttca gtgcagccgc caactaccag cccatccttt
     1681 ttgccagcga gcgctatccg ctgtacttgc ccaccacctt ccgcaaactg gcctcgcgcc
     1741 tgggcgtgag cgttacttcc gaagccgcgg acggtttgca tgtcaagcac agcgtgctca
     1801 actatccact ggttccgcta cgtgtcgagc cgcctgcacg tcccctcaat atcgtctggc
     1861 tggctgtcga gtcgctgcgc tacgacatgc ttgaccgcac gatcatgccg cagctttggg
     1921 atttttccga acatgccctg cgcctcgagc agcactacag tggcggcaac accacccaga
     1981 tgggcatttt ctcgatgttc tacggcttgt acggcaacta ctggtttccg atgcaggccg
     2041 cgcgccggtc ccccctgctg atggacgtat tgcagcaaca gaactaccag ttcagtctgc
     2101 ataccagcca gagtttcacc tatcctccgt ttcaggaaac cgtttttgcc cggatgaacc
     2161 cggccgacat gcacgccctg tccgaggggc cacccccctg gcagcgcgac cggcagaaca
     2221 tcgacgacat cctgaaattc atcgacagtc gtgatcgatc caggccgttc atgacctaca
     2281 tgttctttga gggtacccat gccaattaca ctttcccgcc ggaaagtgtc atcgcccgac
     2341 cgtatctgga cgatttcaac tatctgtcgg ccgattttgc ccagcagatg gtgccgatca
     2401 agaaccgcta catcaacgct gcccaccacg tcgaccagca gatcggacgc atcatcgccc
     2461 atctgcggac
//
LOCUS       DAOSIT010000352         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37060_length_2455_cov_12.044167, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000352 DAOSIT010000000
VERSION     DAOSIT010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37060_length_2455_cov_12.044167"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLL14_08600"
     CDS             <1..280
                     /locus_tag="PLL14_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04029.1"
                     /translation="IRTACRLYPNPEKWFILRRNRSTRRNIRGSPSKPAASPSDVLPL
                     PPGLRNHGALAPCIQEIPPRRSPATQAPAVEPDNTSPSRFGGRTRTIP"
     gene            277..837
                     /locus_tag="PLL14_08605"
     CDS             277..837
                     /locus_tag="PLL14_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HOG04030.1"
                     /translation="MKQAHPSLLSRISLAFGTFFSILSDARFAASVQALRAGEQRAEP
                     SASAPAPRAATVPLKEAPPDGALQLLGLLQREARFIDFIQEDVAAYADADVGAAARVV
                     HEGCRKVLRDNFTISPVRDEAEGSRITLPEGFDATAIRVTGNVVGNPPFHGSITHRGW
                     RVVDTRLPKLTGSHELRIVAPAEVEL"
     gene            834..>2455
                     /locus_tag="PLL14_08610"
     CDS             834..>2455
                     /locus_tag="PLL14_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG04031.1"
                     /translation="MSEPQFSIGIDLGTTHCALSYVDIAACDGEKVVQGVLPVPQLTA
                     PGAVEALGLLPSFIYLPHPSELAAGDLNLPWTAEQDYAVGEMARSRGAATPIRLVSSA
                     KSWLCHAGVDRRAAILPNDAPPEVARLSPLEASLRYMSHLRAAWDQAQPAAPFVDQEI
                     VVTIPASFDPAARELTAEAARAAGCARMLLLEEPQAALYSWIEKSAGGWRKQVRPGDI
                     ILVVDVGGGTSDFSLIAVLEREGTLELHRVAVGEHILLGGDNMDLALAYAVAGKLAAQ
                     GQKLDAWQLRALTHACRNAKETMLSDPDVLTVPLVIASRGARLIGGSTRSELTRAELA
                     ATLLEGFFPPAAIADRPQGRGRGGLTQLGLPYAQDAAVTRHLAAFLARQVAATADLEG
                     FSGRLPDDATFLHPTAVLFNGGVFKSELLAERTLTTVNSWLAAEGAPAARLLEGADLD
                     LAVARGAAYYGYVRRGQGVRIRGGTARSYYVAVESVMPAVPGLQPPVQALCLAPFGME
                     EGSEAAVSTQEFGLVVGEQVRFRFFGSSVRRQ"
BASE COUNT          381 a          914 c          788 g          372 t
ORIGIN      
        1 gatccggacg gcgtgccggc tctaccccaa tcccgaaaaa tggtttatat tgcgccgcaa
       61 cagaagcacc cgcaggaaca ttcgcggatc gccttccaag ccggcggcgt ccccgtcgga
      121 cgtgctgccg ctgccgcctg gcctgcgcaa tcacggcgcg ctggcgccct gcatccagga
      181 aattccgccg cgccgctcgc ctgcaaccca ggcgccagca gtcgagcccg acaacacctc
      241 accgagccgc ttcggcggta gaaccaggac cattccatga aacaagccca tccatccttg
      301 ttgagcagaa tctcccttgc cttcggcacc tttttctcga tcctctccga tgcccgcttc
      361 gctgccagcg tacaggccct gcgtgccggc gagcaacgcg ccgagccatc ggcgtcggcg
      421 ccggcacccc gggcggccac cgtgccgctc aaggaagccc cccccgacgg cgccttgcaa
      481 ctgctcggcc tgctgcagcg cgaggcgcgc ttcatcgatt tcattcagga agacgtcgcc
      541 gcttatgccg acgccgatgt cggcgccgcg gcgcgggtgg tacacgaggg ctgccgcaag
      601 gtcctgcgtg acaacttcac gatcagcccg gtccgcgacg aagccgaggg cagccggatc
      661 acgctgccgg agggctttga tgccacggcg atccgtgtca ccggcaacgt cgtcggcaat
      721 ccgccgttcc acggcagcat cacccaccgc ggctggcgag tggtcgacac ccgcctgccc
      781 aaactcaccg gcagccatga gctgcgcatt gtcgcgccgg cggaggtgga actatgagcg
      841 agccgcaatt ttcgatcggc atcgacctcg gcaccacgca ttgcgccctg tcctacgtcg
      901 acatcgccgc ctgcgacggt gaaaaagtcg tccagggggt attgccggtg ccgcaactga
      961 ccgcccccgg cgcggtggag gccctcggcc tgctgccttc gttcatctac ctgccgcatc
     1021 cgagcgaact ggcggcgggt gatctgaacc tgccgtggac cgccgaacag gactacgcgg
     1081 tcggcgagat ggcgcgcagc cgcggcgcgg caacgcccat ccgactggta tcgagcgcca
     1141 agagctggct ctgtcacgcc ggcgtagacc gccgcgcggc gatcctgccc aacgacgccc
     1201 cgccggaagt ggcgcgtctg tcgccactcg aagcctcgct gcgctacatg agccatctgc
     1261 gcgccgcctg ggatcaggcg cagcccgcgg ccccgttcgt cgatcaggag atcgtggtga
     1321 ccatccccgc gtccttcgac ccggcggcgc gcgaactgac cgccgaagcg gcccgtgccg
     1381 ccggctgcgc gcgcatgctc ctgctcgaag aaccgcaggc cgcgctgtac agctggatcg
     1441 agaagagcgc cggcggctgg cgcaagcagg tgcgaccggg cgacatcatc ctggtcgtcg
     1501 atgtcggcgg cggcaccagc gacttctcgc tgatcgccgt cctcgaacgc gaaggcacgc
     1561 tcgaactgca ccgcgtcgcc gtcggcgagc acatcctgct cggcggcgac aacatggatc
     1621 tggccctggc ctatgccgtc gccggcaaac tcgccgcgca gggacagaaa ctcgacgcct
     1681 ggcaactgcg ggcgctgacg cacgcctgcc gcaacgccaa ggagacgatg ctcagcgatc
     1741 cggacgtact cacggtacca ctggtgatcg ccagccgcgg cgccaggctg atcggaggat
     1801 cgacccgcag cgaactgacg cgtgccgaac tcgcggcgac gctgctcgaa ggtttcttcc
     1861 cgccggcggc catcgccgac cggccgcagg gacggggccg tggcggcctg acgcaactcg
     1921 gtctgcccta cgcgcaggat gccgcggtca ctcgccatct ggccgctttc ctcgcccggc
     1981 aggtcgccgc cacggccgac ctcgagggtt tcagcggccg cctgccggac gacgcgacct
     2041 ttctgcatcc caccgcggtg ctgttcaatg gcggcgtctt caagtccgaa ctgctcgccg
     2101 aacgcacgct gacgacggtc aattcctggc tcgccgccga gggtgccccg gccgcccgtc
     2161 tgctcgaagg cgccgacctc gacctggcgg tggctcgcgg cgccgcctac tacggctacg
     2221 tccgccgcgg ccagggcgtg cgcatccgcg gcggcaccgc ccgttcctac tacgtcgcgg
     2281 tcgaatcggt gatgcccgcg gtgcccggcc tgcaaccgcc ggtgcaggcc ctgtgcctgg
     2341 cgcccttcgg catggaggaa ggcagcgaag ccgcggtgtc gacgcaggaa ttcggtctgg
     2401 tcgtcggcga gcaggtccgc ttccgcttct tcggctcgtc ggtccgccgc cagga
//
LOCUS       DAOSIT010000353         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37094_length_2453_cov_8.814429, whole genome shotgun sequence.
ACCESSION   DAOSIT010000353 DAOSIT010000000
VERSION     DAOSIT010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37094_length_2453_cov_8.814429"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLL14_08615"
     CDS             <1..744
                     /locus_tag="PLL14_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG04032.1"
                     /translation="AAAEAVNRFPEVNHNYEREHRYNLWLVVTAGSEGRLQAALGAIE
                     QAAGWPLLRLPLLQEYHIDLGFPLNGKGERPAASGGVRWQPFVPPMPLDEGERRLVSA
                     LQEGLPLFIRPFAVLASRVGCDESEVLERIRRWCAEGIIKRFGVIVRHRELGYTANAM
                     LVHDIPDADVGEIGERLACAAGVTLCYQRPRVPPDWPYNLFCMIHGQDRAQVEASIAE
                     LRARLSLTEYAHDTLFSLTRFKQRGAHYA"
     gene            737..1216
                     /locus_tag="PLL14_08620"
     CDS             737..1216
                     /locus_tag="PLL14_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOG04033.1"
                     /translation="MREAIELDTIDRAIIDTLQGGFPICERPYAEVAGRLGIAEADLL
                     WRLQRLLDRRVLTRFGPLFQIERMGGTFVLAAMRVPESDWQRVLAVVNSLHAVAHNYR
                     RESERNSEFNMWFVLAAESAAGIDVAIAQIQQRSGLQVFRFPKLQEYFVEMKLPVRP"
     assembly_gap    1221..1320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1321..1539
                     /locus_tag="PLL14_08625"
     CDS             <1321..1539
                     /locus_tag="PLL14_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG04034.1"
                     /translation="VGALDFVTHCYHRPRRLPEWPYNLFAMVHGSSRAEVRGMVAAIA
                     AVLGCDCRSYDILFSTAILKKTGLRIGA"
     gene            1661..2164
                     /locus_tag="PLL14_08630"
     CDS             1661..2164
                     /locus_tag="PLL14_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG04035.1"
                     /translation="MAIAFPDRGREVELEEGWQMGSAQVGRGFLRHPADIPIEIDAEV
                     PLPTRLRRLKDISLGGLACRSERPLEVGSGVRLTIPLVKPAFHAAGSVVWCRRHGLYY
                     EVGIRFIESDDVFAARMVEQICQIEHYRKEVLHSEGRVLDGEAAALEWIAHYAAKFPS
                     LDTTSRH"
     gene            complement(2249..>2453)
                     /locus_tag="PLL14_08635"
     CDS             complement(2249..>2453)
                     /locus_tag="PLL14_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel protein"
                     /protein_id="HOG04036.1"
                     /translation="ALLLVLVFWVELGPNLVARRVDSDLRHAIGQRLEAAGIPIPFPQ
                     RDVHLDISQPLPVRLTRTPADNA"
BASE COUNT          401 a          703 c          780 g          469 t
ORIGIN      
        1 gctgccgccg aggcggtgaa tcgcttcccc gaggttaacc acaattacga gcgcgagcac
       61 cgctataatc tgtggctggt cgtcacggcc ggttcggaag gccgcctgca ggccgccctc
      121 ggcgccatag aacaggcggc tggctggccg ctgctgcgct tgcccctgct gcaggagtac
      181 cacatcgatc tcggtttccc gctgaatggc aaaggggaga ggccggccgc cagcggtggc
      241 gtacgctggc agcccttcgt gccgccgatg ccgctggatg agggtgagcg tcgtctggtc
      301 agtgccttgc aggaaggctt gccgctgttc attcgtccgt tcgcggtcct tgccagtcgt
      361 gtcggctgcg acgaaagcga ggttctcgaa agaatccgtc gctggtgcgc cgaaggcatc
      421 atcaagcgtt tcggagtcat cgtcaggcat cgtgaactcg ggtacacggc caacgcgatg
      481 ctggttcacg acatccccga cgccgatgtc ggcgagatcg gcgagaggct ggcctgtgcc
      541 gccggagtga cgctctgcta ccagcgtccg cgggtgcctc ccgactggcc ctacaatctg
      601 ttctgcatga ttcacggtca ggatcgcgcg caggtcgagg cgagtatcgc cgaactgcgc
      661 gccaggctca gtctgaccga atacgcgcat gacacgctgt tctcgctgac ccgtttcaag
      721 cagcgcggcg cgcattatgc gtgaggcgat cgaactcgac accatcgatc gcgcgatcat
      781 cgacacgctg cagggtggtt ttccgatctg cgagcggccc tatgctgaag tggccgggag
      841 gctcgggatt gccgaggcgg acttgctctg gcgcctgcaa cgcctgctcg accggcgcgt
      901 gctgactcgc ttcggcccgc tctttcagat tgaacgcatg ggtggcacct tcgttctggc
      961 ggcgatgcgc gtccctgaat cggactggca gcgcgtgctc gcggtcgtca attcactgca
     1021 cgcggtcgcc cacaactacc gtcgcgagag tgagcggaac agcgagttca acatgtggtt
     1081 cgttcttgcc gccgagagcg cggccggtat cgatgtggcg atagcgcaga ttcaacagcg
     1141 cagtggtttg caggtcttcc gcttcccgaa gctgcaggag tatttcgtcg agatgaaact
     1201 gccggtgcgg ccatgacggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 gtcggagcgc tcgatttcgt cacccactgc tatcaccggc cgcgccgact tcccgagtgg
     1381 ccgtacaacc tgtttgccat ggtgcatggt agttcacggg cggaagtgcg gggcatggtg
     1441 gcggcgatcg cggcggtcct cgggtgcgat tgccgttcgt acgatatcct gttttcgacc
     1501 gccattctca agaaaacggg cctgcggatc ggcgcttgag cagagcttcc ctgcgcgtag
     1561 cgaacccgaa ccactgctgc actgccgatc gatatctgcc gtacaattcg accgtcggtg
     1621 ctcaggagca acaggcctgc gtacttgcct gatggagctg ttggcgatcg ctttccctga
     1681 tcggggtcgg gaggtcgaac tggaggaggg ctggcagatg ggatcggcac aggtgggacg
     1741 tggttttctt cggcatccgg ccgacattcc gatcgagatc gatgccgagg tgccgttgcc
     1801 gacgaggttg cgtcgtctga aggacatcag cctcggtggg ttggcctgtc gctcggagcg
     1861 gccactggag gtcgggtctg gtgtacggct gaccatcccg ctggtcaagc cggcctttca
     1921 cgcggcgggt tcggtggtat ggtgccgtcg ccatggtctg tactacgaag tcggtatccg
     1981 gttcattgaa tccgatgatg ttttcgctgc ccgcatggtg gagcagatct gccagatcga
     2041 gcactaccgc aaggaagtgt tgcacagcga aggacgagtg ctcgacggcg aggccgcggc
     2101 gctggagtgg atcgctcatt acgccgcgaa gtttccatcc ctggacacga ccagtcgcca
     2161 ctgaggatct gcaatgccga tcaccgtcag cgctgcggta cgcatcggcc aagggccggt
     2221 aacgccggcc agcaatcgtg caggggtttc aagcgttgtc ggcgggggtt cgcgtcagcc
     2281 gtaccggtaa cggttgtgaa atgtcgagat gcacatcacg ctgcgggaac ggaatcggaa
     2341 ttcctgcggc ttccagacgc tgcccgatcg catgccggag gtcgctatcc accctgcggg
     2401 caaccagatt cggccccagt tcgacccaga agacgaggac caacagcagc gca
//
LOCUS       DAOSIT010000354         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37205_length_2447_cov_7.419732, whole genome shotgun sequence.
ACCESSION   DAOSIT010000354 DAOSIT010000000
VERSION     DAOSIT010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37205_length_2447_cov_7.419732"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLL14_08640"
     CDS             complement(<1..545)
                     /locus_tag="PLL14_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG04037.1"
                     /translation="MNIFSKFGNQKIWVRLIVSISFMTIASWAVMILWTAHVSEEAAI
                     EQAQEFARSAHDMVLAGLTGMMVTGTIPQREVFIDQIKQLPSIREVRVLRGEAVTRQF
                     GPGIRQESEHDALEGEVLATGKEYSAVESTAKGEEALRVIRPALAHENYLGKNCMTCH
                     AVPLGTVLGAVSMKVSLKSTR"
     gene            complement(794..1294)
                     /locus_tag="PLL14_08645"
     CDS             complement(794..1294)
                     /locus_tag="PLL14_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOG04038.1"
                     /translation="MSPLSQIHAMIDARVRAIRKDRPDWLCGKGCDTCCRRLAELPQL
                     TAAEWGLLRDGLATLSPQRLDEIGRDLAALARHGARPLVCPLLEPSTGTCPVYAQRPV
                     ACRSYGFYVQRQLGLYCPEIEARVADGSLTDVVWGNHDAIDQRLADLGESRPLTEWFG
                     SWWYGQ"
     gene            complement(1335..2108)
                     /locus_tag="PLL14_08650"
     CDS             complement(1335..2108)
                     /locus_tag="PLL14_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04039.1"
                     /translation="MSANCRVPLLAFLLLLPMLANGACTINDKQWLWNYSGSIGEKYR
                     IGMTLVLAGSAVSGSYFYASQLADIRLRGRIAHGTDIVLDELDAADKVVARFEGRFLT
                     SDPSGRYQGDKLECEIITGTWHKLDANEGLPVRLTLDNGTAGSLANRYVVAGATDDER
                     VHRQALRFWHAVKRADKPTVAALLAYPLNVVVAGSRKRINDAKELIANYDAIFSPQMR
                     AAIGNALPRNMFVRDQGIMLGNGEVWFGADGKVIALNPP"
     gene            complement(2191..>2447)
                     /locus_tag="PLL14_08655"
     CDS             complement(2191..>2447)
                     /locus_tag="PLL14_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOG04040.1"
                     /translation="IYDLWGDTVNIAARMESQGVSGGIQISEATRALLPTDFVCEDRG
                     EIAVKGKGAMRVHILKGRATATASAACNALIEPPKFLEQS"
BASE COUNT          438 a          778 c          727 g          504 t
ORIGIN      
        1 tccctggtcg atttcagcga aaccttcatg cttaccgcac ccaggacggt gcccagcggc
       61 acggcatggc acgtcatgca gttcttgccc agatagttct cgtgcgccag cgcgggccga
      121 atgaccctga gggcctcctc gcccttcgcg gtgctttcga ccgcggaata ctccttaccc
      181 gttgcaagaa cctcgccttc cagagcatcg tgttcacttt cctgcctaat cccgggacca
      241 aactgcctgg tcaccgcctc gccgcgcagg accctgacct cgcgaatgct cggcaactgc
      301 ttgatctggt caatgaacac ctcgcgttgt ggaatcgtgc cggtgaccat cattccggtc
      361 agcccggcga gcaccatgtc gtgcgcactc cgagcgaact cctgcgcctg ctcgatcgca
      421 gcctcttcgc tgacgtgcgc cgtccacagg atcatcacgg cccaggacgc gatagtcatg
      481 aagctgatcg agacgatcag gcgcacccag atcttctgat taccgaattt cgaaaaaatg
      541 ttcatgaatg aggttgcctt gtaacccgtt caacttattc ttgcaggcca ggcgagcgcg
      601 acaccactac gccccggccc attttccccg cccaagttcc gataacgctt ctacgtctgc
      661 cttctgtagg ccccgtgcga aaaaatgttc tccaccgtga ttgtcgcacg cagacatgcg
      721 ggccggaatc ccattagtgg cggtgacccg gttgctgcat ttcggcagtt ccggttgcat
      781 cgggctggct acgctattgg ccataccacc aacttccgaa ccactcagtc aggggacggc
      841 tttcgcccag gtcggcgagc cgctggtcga tggcatcgtg gttgccccac accacgtcgg
      901 tgaggctgcc gtcggcaacg cgcgcttcga tttccggaca gtacagtccg agttgccgct
      961 gcacgtagaa gccgtagctg cggcaggcca ccggacgctg cgcatagacc gggcaggtgc
     1021 ctgtggatgg ttcaagcaac ggacagacca gcgggcgcgc accgtgccgg gccagagcgg
     1081 ccaggtcgcg gccgatttcg tccagccttt gtggagacaa cgtggccaga ccgtcccgca
     1141 gcaggcccca ttccgctgcc gtcagttgtg ggagttcggc cagccgtcga caacaggtat
     1201 cgcaaccctt gccgcacagc cagtcggggc gatcctttcg gatggcgcgc acgcgcgcgt
     1261 cgatcatggc gtggatttgg gaaaggggac tcatcagcgg cgtgcccgca ccaacggcct
     1321 actggcggga gatttcacgg cgggttcagg gcgatcacct tgccgtcggc accgaaccac
     1381 acctcgccgt ttcccagcat gattccctgg tcgcgaacga acatgtttcg cggcagtgcg
     1441 ttgccgatcg ctgcgcgcat ctgcggggaa aagatcgcgt cgtaattggc gatcagttcc
     1501 ttcgcatcgt tgatgcgctt gcgactgccg gcaacgacga cgttgagcgg gtacgcaagg
     1561 agcgcggcga ctgtcggctt gtcggcgcgt ttgacggcat gccagaagcg cagggcctgg
     1621 cggtgtacgc gttcgtcgtc cgttgctccg gcaacgacgt aacgattggc caggcttccc
     1681 gcagttccat tatctaatgt caggcgcacg ggcaatcctt cgttggcatc gagcttgtgc
     1741 caggtgccgg tgatgatctc gcattccagc ttgtccccct gatagcggcc gctcgggtcg
     1801 ctggtcagaa agcggccttc gaaacgggca acgaccttgt ccgcagcgtc cagctcgtcg
     1861 aggacgatgt ccgtcccatg cgcaatcctt ccccgcaacc tgatgtccgc cagttgactg
     1921 gcgtagaagt agctgccgct gacagcgctg ccggcaagca cgagcgtcat accgatgcgg
     1981 tatttttcgc cgatgctccc cgagtagttc cacagccact gcttgtcgtt gatagtacac
     2041 gcgccgttcg ccagcatggg gagcagcaga aggaaagcaa gcagtggcac tcgacagtta
     2101 gcggacattg gcgtttgcgg ctcctgtggg gggaatcggg tgctcgcccg atcatgttaa
     2161 ttgaatctgc accgctcgtt cacccagtac tcagctttgt tccaggaatt tcggaggttc
     2221 tatgagtgca ttgcaagcgg ccgatgctgt ggcggttgcg cggcccttga ggatatggac
     2281 gcgcatcgca cccttgccct tgaccgcgat ttcgccacga tcctcgcaga cgaagtcggt
     2341 gggcaacagg gcacgcgtcg cttcgctgat ctggatcccg ccggagaccc cttgcgattc
     2401 catgcgcgcg gcgatattga ccgtgtctcc ccagaggtcg tagatga
//
LOCUS       DAOSIT010000355         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37246_length_2445_cov_8.441004, whole genome shotgun sequence.
ACCESSION   DAOSIT010000355 DAOSIT010000000
VERSION     DAOSIT010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37246_length_2445_cov_8.441004"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLL14_08660"
     CDS             complement(<1..449)
                     /locus_tag="PLL14_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HOG04041.1"
                     /translation="MTAARKRDDGFDGTTAMRAGAQGTLVRRVIGICGMAGVLMLGGC
                     ADMMGTGSNVTVSDPTRITHTGFLSDYSRLTRAPGGDGALCWRNPELDMRRYDKVLIN
                     RIMVTLKPDQQASVDPTDLKTLVDYFHNSLVKALKPQMQVVGQPGPGV"
     gene            complement(509..>2445)
                     /locus_tag="PLL14_08665"
     CDS             complement(509..>2445)
                     /locus_tag="PLL14_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG04042.1"
                     /translation="GIWEYEPAGRRVHWDASMYALYGVDALPPVTTYDEWRGTVLPED
                     LPTVEAALQASIRERKQFRGDFRIRRGDGQIRMISALARPLLPADGTTVRVVGINQDV
                     TERWQAEAAALASEAYLKAIFDVVPIGISVLDQTGRIVDCNATAERLLGVSREQPFPD
                     RSDSAFWTVRRSDGSVMPREECAWVRALASGVAVFDQDLLVEMPTAGHWLSVSAVPIA
                     TSPRGRAVADGELATECGRESGGVVIACVDVGAARRNEAELRQLSRAVEQSAAAVLIT
                     DIDGIIEYVNPAACEAYGYSSAELLGANPRLLGSGLTPISTYQAMWSTILAGKAWRGE
                     LANRRRDGSLIHEAVSISPVCDRAGQMTHFVSIQEDVSARVEAEHLQDELKTRLGRVA
                     RMDVLGVMAGGVAHDFNNILVAILGFSGLGKTVLRATCGAERLVSYFEEIEAAGERAR
                     ALVQQLLVFSRGGALKLAVVPVTAVAGEVLSLLAPSLPTSVTLCTDIDDDLPLLDVDR
                     SHLHRLLVNLCLNARDAMEGPGAVVISARLVCQQAAAVCASCHGEFSGEFLRLSVSDQ
                     GRGIPEAIRDRIFEPFFTTREVGAGSGMGLAVVHGLTHLYEGHLQVFSVPEQGSEMVI
                     LLPRSMWHVDAAAASGAGEH"
BASE COUNT          482 a          853 c          702 g          408 t
ORIGIN      
        1 accccgggtc cgggctgacc gacaacctgc atctgcggct tcagggcctt gaccagcgaa
       61 ttgtggaagt aatcgacaag cgtcttgaga tcggtcggat ccaccgacgc ctgctggtca
      121 ggcttgaggg tgaccatgat ccgattgatc agtaccttgt cgtagcgccg catgtcgagt
      181 tcagggttcc gccagcacaa cgcgccgtcg ccgccagggg cgcgcgtcag ccggctgtag
      241 tcgctgagaa aaccggtatg agtgatccgt gtcggatcgc tgacggtaac gttgctccca
      301 gttcccatca tgtcggcaca tccacccagc atcaacaccc cggccatgcc acagataccg
      361 atgacgcgac ggacgagtgt cccttgcgca ccggcacgca tggcggttgt gccgtcaaat
      421 ccatcgtcgc gtttccttgc cgctgtcatt ctcatacctt tctggttgga aaggcgcatt
      481 gtacgccgtt gctactattg cgccgcactc agtgttcgcc cgcaccagac gcggccgccg
      541 cgtcaacgtg ccacatgctg cgtggtagca ggatcaccat ttcgctgcct tgctccggca
      601 cgctgaacac ctgcagatgc ccttcataaa gatgggtcag gccgtgcacc accgcaagcc
      661 ccattcccga accggctccc acttccctgg tagtgaagaa cggctcgaaa atgcggtcgc
      721 ggatcgcctc cgggatacca cgcccctggt cgctgaccga gagacgcagg aactcaccgg
      781 aaaactcccc atggcaggag gcacagacgg ctgccgcctg ctggcagacg agccgcgccg
      841 aaatcaccac cgcgcccgga ccttccattg cgtcgcgggc gttcaggcag agattgacca
      901 gcaggcgatg caggtgcgaa cgatcgacat cgagcaacgg caggtcatcg tcaatgtcgg
      961 tacacagggt caccgaagtg ggtagcgatg gggcaagcag actgagcact tcgcccgcca
     1021 ccgcggtcac gggcaccacg gccagcttga gcgcgccacc gcgcgagaag accagcaatt
     1081 gctgcaccag ggctcgggca cgctcgccgg cggcttcgat ctcctcgaaa tacgacacca
     1141 ggcgctcggc accacaggta gcccgcagca cggtcttgcc cagccccgaa aaaccgagaa
     1201 tcgccaccag gatgttgttg aagtcatgcg cgacgccacc ggccatcacc cccagcacgt
     1261 ccatgcgcgc aacccgcccc aggcgggtct tcagctcgtc ctggaggtgc tcggcctcga
     1321 cccgcgccga gacatcctcc tggatggaca caaaatgcgt catttgtccc gcccgatcac
     1381 acactggcga aatcgaaacc gcttcgtgga tcaggctacc gtcacgccgg cggttggcca
     1441 actcgccacg ccaggctttg ccggcgagaa tcgtcgacca catcgcctga taagtcgaga
     1501 tcggggtcaa tcccgaccca aggagccgcg ggttggcgcc caacagttcc gcgctgctgt
     1561 aaccataggc ctcgcaggct gccggattga cgtattcgat gatgccatca atgtcggtga
     1621 tcaacaccgc cgctgctgat tgctccaccg cccgcgagag ctggcgcaac tcggcttcat
     1681 tgcggcgcgc agcgccgaca tccacgcagg caatcaccac ccctccagat tcccgaccgc
     1741 attcagtcgc caactcgcca tcggcgaccg cgcggccgcg cggggaagtg gcgataggca
     1801 cggcactcac cgacaaccag tgtcccgctg tcggcatctc cacgagcaga tcctgatcga
     1861 acaccgcgac accactcgcc agagcgcgta cccaggcaca ctcttccctg ggcatgacgc
     1921 tgccgtcgga gcgccgaacg gtccagaaag ccgaatcgga acggtcgggg aacggctgct
     1981 cgcggctcac acccagcaat cgctcggcgg tggcattgca gtcaacgatg cgccccgtct
     2041 ggtccagcac cgatatgccg atcggaacca cgtcgaaaat cgctttcaga taagcttcgc
     2101 tggcaagtgc cgcagcctcg gcttgccagc gctcggtcac gtcctgattg atgcccacaa
     2161 cgcgtaccgt cgtcccgtcc gccggaagaa gtggccgcgc cagcgcgctg atcatgcgga
     2221 tctgcccgtc gccgcgacga atgcgaaaat cgccacggaa ctgtttgcgc tcgcgaatcg
     2281 acgcttgcaa cgccgcttcc accgtaggca gatcctccgg gaggaccgtt cccctccact
     2341 cgtcgtaggt cgtcaccggt ggcagggcgt cgacgccgta cagcgcgtac atcgacgcat
     2401 cccaatgcac gcgcctaccg gcgggttcgt actcccagat gccca
//
LOCUS       DAOSIT010000356         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37329_length_2441_cov_6.411987, whole genome shotgun sequence.
ACCESSION   DAOSIT010000356 DAOSIT010000000
VERSION     DAOSIT010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37329_length_2441_cov_6.411987"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2441)
                     /locus_tag="PLL14_08670"
     CDS             complement(<1..>2441)
                     /locus_tag="PLL14_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOG04043.1"
                     /translation="EFTHFFAIILWIGAGLAFIAEHFDPGQGMARLGVAIVGVIVVNG
                     IFSFWQEYKAERAVAALRQLLPQKVKTLRGGEIVDMLASELVPGDIVLLEEGDYVPAD
                     CRLIEVFGLRVNLSTVTGESLAKARNVEPSTEESALQAKNVVLAGTSVVSGQGRGVVF
                     ATGMHTEFGRIAHLTQTAGATTSPLQREIARLSRIVASLATGMGVLLFCVGQAMGLPT
                     WENLLFAIGIIVANVPEGLLPTVTLSLAMATQRMARRNALVRHLPAVEALGSTTVICS
                     DKTGTLTQNHMSVQQLWVGGAFLPRADLEKQENLIPEHRELFINAALCHNLKDIDVHG
                     QHTQHGDPMEIALAEIGREMVGYLQGFSRVDEIAFDTDRKRMSVLCETPQGRMLYCKG
                     ALETVLATCRFIHFDAGLATIDPAARTRLLAAQDEMAGAGLRVIAFAHRAVVDGAVPD
                     DEHEMILAGLIGLEDPPRSAVPEAIARCTAAGIRIIMVTGDHPRTALAISRQIGLVHS
                     EQVSVISGEQLRLMSPTQLQLALDAPEIIFARVAAEQKMRIVEALKKKGEIVAVTGDG
                     VNDAPALKTADIGIAMGISGTDVAKSAADLILLDDNFASIVSAIEEGRAVFENIRKFL
                     TYILSSNIPELVPYLAFVLFRIPLPLTILQILAVDLGTDMLPALALGAEKPDPGVMTR
                     PPRARSERLLSWGLLARAYLFLGMLEATAAMLAFFFVLDTGAWHYGEGLDRADPLYLQ
                     ATAACLATIVVMQMINLYLCRHPRKSSLTYGLGGNPYLQFGILAELAVLVFILYAPAG
                     NWLFGTAPISGASWL"
BASE COUNT          464 a          741 c          791 g          445 t
ORIGIN      
        1 cagccagctg gcgccgctaa tcggcgcggt gccgaacagc cagttgccgg caggggcgta
       61 caggatgaag acgagcactg ccagttcggc gagaattcca aactgcaggt aagggttgcc
      121 gcccaggccg taggtgagcg aggacttgcg cgggtgccgg cagaggtaga gattgatcat
      181 ctgcattaca acgattgttg ccaggcaggc cgccgtcgcc tgcaggtaga gcgggtcggc
      241 gcggtccaga ccctcgccgt aatgccaggc accggtgtcg agtacgaaaa agaacgccag
      301 catagccgcg gtcgcttcga gcatgccgag gaaaagatag gcacgcgcga gcagacccca
      361 ggacagcaag cgctcgctac gcgcgcgcgg cggcctggtc atcactcccg ggtcgggttt
      421 ctcggcgccg agtgccagcg cgggcagcat gtcggtgccc aggtcgacgg ccaggatctg
      481 aagaatggtc agcggcagcg ggatcctgaa cagcacgaag gcgagatagg gtaccagttc
      541 cgggatgttc gaactgagga tgtaggtgag aaacttgcgg atgttctcga ataccgcgcg
      601 tccctcttcg attgcggaaa cgatgctggc gaagttgtcg tcaagtagta tcaggtcggc
      661 cgcagacttc gcgacgtcgg tgccgctgat acccatggcg atgccaatat ctgcggtctt
      721 caatgccggg gcgtcgttga ccccgtcgcc ggtgaccgcg acgatctcgc ctttcttctt
      781 gagcgcctcg acgatgcgca ttttctgctc ggctgcaacg cgggcgaaga tgatttccgg
      841 cgcatccagt gccagttgca gttgcgtcgg ggacatcaaa cgcaactgct cgccgctgat
      901 cacggacact tgttcgctgt ggacgaggcc gatctggcgg ctgatcgcca gtgccgtacg
      961 cggatggtcg ccagtgacca tgatgatccg gatgcccgcc gccgtgcagc gcgcgatggc
     1021 ttccggcacc gcgctgcgcg gtggatcctc caggccgatc agcccggcga ggatcatttc
     1081 gtgttcatcg tcgggaacag cgccatcaac gaccgcgcga tgtgcgaaag cgatcacccg
     1141 cagaccggcg ccagccattt cgtcctgtgc cgccagcaaa cgtgtgcggg cggcgggatc
     1201 gatcgttgcc aaaccggcgt cgaagtggat gaaccggcag gtggcgagca ccgtctcgag
     1261 ggcacccttg caatacagca tgcggccctg cggtgtttcg cagagtaccg acatgcgctt
     1321 gcggtcggta tcgaaggcga tttcgtcgac gcggctgaaa ccctgaagat atccgaccat
     1381 ttcccgcccg atctcggcga gcgcaatttc cattggatca ccgtgctggg tgtgttgacc
     1441 atgcacgtcg atgtccttca ggttgtggca cagagcggca ttgatgaaga gttcacggtg
     1501 ttcaggtatc aggttctcct gtttctccag atcagcgcgc ggcaggaacg ccccaccgac
     1561 ccacaattgc tgtaccgaca tgtggttctg ggtcagcgtg ccggtcttgt cgctgcagat
     1621 caccgtcgtc gaacccaacg cctcgactgc cggcagatgg cgcaccagcg cattacgcct
     1681 ggccatccgt tgtgtggcca tcgccagcga gagggtcact gtgggcagca atccttccgg
     1741 gacattggcg acgatgatcc cgatcgcgaa caacaggttc tcccaggtcg gcagccccat
     1801 tgcctgacca acacagaaca gtagcacgcc catgccggtg gccaggctgg ccacgatccg
     1861 cgagaggcgc gcgatttcgc gttgcagcgg tgaagttgtt gcgcctgcgg tctgcgtcag
     1921 atgggcaatg cgtccgaatt cggtgtgcat gccggtggcg aagacgacgc cgcggccctg
     1981 tccggaaacg accgaggtgc cggcaagcac gacgtttttt gcctgcaatg cggattcctc
     2041 ggtgctgggc tcgacgttgc gcgccttggc cagcgattcg ccggtgaccg tagacaggtt
     2101 gacgcgcagg ccgaagacct cgatcagccg gcagtcggcg ggaacgtagt ctccttcctc
     2161 gagcagcacg atgtcgcctg ggaccagttc gctggccagc atatcgacga tctcgccgcc
     2221 acgcagggtc ttgaccttct gcggcagcaa ctggcgcaac gcagcgacgg cgcgttcggc
     2281 cttgtactcc tgccagaacg agaaaatgcc gttgaccacg atgacgccga cgatcgccac
     2341 accgaggcgc gccattccct gacccgggtc gaaatgttcg gcgataaagg ccagaccggc
     2401 gccgatccac aggatgatgg caaagaagtg ggtgaactcg c
//
LOCUS       DAOSIT010000357         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37444_length_2435_cov_8.535294, whole genome shotgun sequence.
ACCESSION   DAOSIT010000357 DAOSIT010000000
VERSION     DAOSIT010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37444_length_2435_cov_8.535294"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="PLL14_08675"
     CDS             <1..2379
                     /locus_tag="PLL14_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOG04044.1"
                     /translation="PELACRALVAEIRRCLDWHGSVWLPLASRLTSEGLKLDELLARI
                     PREASPISEYLAIERLVSSILPATLVAEVGRRKLRECELGFKHLANLATQVDPTTANR
                     GCLDRIVDAVHLRSPQAYAAALDYARRLQMVKPLVTERDALAGRLRLVAAGWSEQITQ
                     RVPPHDAGTIPGDFAMAWTWRQIHDTLVERDRLDAHALQGQIDQQRESLRQVTEQLID
                     ASAWGKQLARLQGNHSIRQALVGWLDTTRRLVSTRQLDRRQDLLSAARKSMKQCAEAV
                     PVWIMPISIMAESFDPGSTRFDVVIVDEASQADLNALIPLYLGKQVIVVGDHEQVTPL
                     GVGQGQAMLDNLRQAMLQDIPNAHLFDHLCSIYDIGRQSFGDAIRLVEHFRCVPEIIA
                     FSNQLSYAGTIRPLRESNSTDIKPACVACRVDGIREGDTNQAEARRIIDTIKAMLRHP
                     TYAGKTIGVISMLGEAQALHIQSLLHKEIPDVEIANRRIQAGISGEFQGDERDIIFLS
                     MVDSPPSEGMLRTTGEGAFELIKKRYNVAASRARDQLWVVHSFDPDRHLRPNDIRLKL
                     LQHVRDPSACLRANEREIGRTESPFERDVLKRLADAGFRVRSQWPVGYYRIDMVVEGT
                     GRRLAIECDGDRYHPMERLAEDMARQAVLERLGWQFVRLRGSAFYRNPEAAMRPVFDR
                     LAVLAIPPAVDVDIHAAQGEDMTLIHELEALIENGLQGDEAPVDTPLDLVEVLLRERR
                     GTAALEPFLHDLARAKGSQRLGPKMRAGLEAELAMLVQQGKVAIGGGDIRLL"
BASE COUNT          461 a          740 c          791 g          443 t
ORIGIN      
        1 ccggaactcg cctgtcgtgc cctggttgcg gagatccgcc gctgcctcga ctggcatggc
       61 tcggtctggt tgccactcgc ctccagattg acgtccgagg gtctgaagct ggatgagctt
      121 ctggcgcgga ttccgcgcga ggccagcccg atttccgagt acctggccat tgaacgcctg
      181 gtttcgtcga ttttgcctgc gacgctggtg gcggaggtgg ggcgacgcaa actgcgcgag
      241 tgcgagcttg gcttcaagca cctggcgaac ctggcgacac aggtcgatcc aacgacggcg
      301 aaccggggtt gcctcgaccg catcgtcgac gcggttcact tgcgcagtcc gcaggcttac
      361 gctgcggcat tggactatgc tcgccgcctg cagatggtga agccgctggt caccgaacgt
      421 gacgcactgg ctggcaggct tcggcttgtc gcagcgggct ggtcggagca gatcactcag
      481 cgcgtgccgc cacacgacgc gggaacgatt ccgggcgatt tcgcgatggc gtggacgtgg
      541 cgacagatcc acgacaccct ggtcgaacgg gaccggctcg acgcccatgc cttgcaggga
      601 cagatcgacc agcagcgcga gagccttcgc caggtcaccg aacagcttat cgacgccagt
      661 gcgtggggca aacagctcgc gcggctgcag ggcaatcact cgattcgtca ggcattggtc
      721 ggctggctgg atacgaccag acgacttgtt tcgacccggc agttggacag gcggcaggat
      781 ctgctctcgg cagcgcgaaa atcgatgaag cagtgtgccg aggcggtgcc ggtctggatc
      841 atgccgattt cgatcatggc cgagagcttt gacccgggtt ccacgcgctt cgacgtggtg
      901 atcgtcgacg aggcgagcca ggccgacctc aatgcgctga ttccgcttta cctcggcaag
      961 caggtgatcg tggttggcga tcacgagcag gtaacgcctc tcggtgtcgg ccaagggcag
     1021 gcgatgctgg ataacctccg ccaggccatg ctgcaggata ttcccaacgc gcatctgttt
     1081 gaccatctgt gctcgatcta cgacatcggc cggcagtcat tcggcgacgc catccggctc
     1141 gtcgagcatt tccgctgtgt tccggaaatc atcgccttca gcaaccagct ctcgtacgca
     1201 ggcacgatcc gtcccttgcg cgagtcgaac tcgaccgaca tcaaaccggc gtgcgtggcc
     1261 tgtcgcgtcg atggcatccg cgaaggtgac accaaccaag ccgaagcgcg acggatcatc
     1321 gacacgatca aggcgatgct gcggcatccg acctacgctg gcaagacgat cggcgtgatc
     1381 tcgatgcttg gcgaggcgca ggccttgcac atccagtcgc tgctgcacaa ggaaattccc
     1441 gacgtcgaga tcgcgaatcg ccgtatccag gccggcatct cgggggaatt ccagggcgac
     1501 gagcgggaca tcatctttct ttcgatggtc gacagcccgc ccagcgaagg gatgctgcgc
     1561 acgaccgggg aaggcgcttt cgagctgatc aagaagcgct acaacgtcgc cgccagccgg
     1621 gcacgcgatc agttatgggt ggttcactcc ttcgatcccg atcgacacct caggccgaac
     1681 gacatccggc tgaaactgtt gcaacacgtc cgggacccgt cagcttgcct gcgtgcgaac
     1741 gaacgggaga tcggcaggac agagtcgccg ttcgagcggg atgtgctgaa gcgtttggcg
     1801 gacgcgggtt tccgtgtcag gtcgcagtgg ccggtcggct actaccggat cgacatggtc
     1861 gtcgaaggca caggcaggcg actggccatc gaatgcgacg gtgatcgcta tcaccccatg
     1921 gagagacttg ccgaagacat ggcacggcag gccgtcctcg aacggcttgg ctggcaattc
     1981 gtccgtctcc gcggcagcgc cttctaccgc aatccagaag ctgccatgcg gccggtattc
     2041 gatcgactgg cggtgttagc gattcccccg gccgtggatg tcgacattca cgccgcgcag
     2101 ggcgaggaca tgacattgat tcacgaactg gaggccttga tcgagaacgg gcttcaaggg
     2161 gatgaggcac cggtcgatac accgctggac ctggtcgaag tcttgctgcg cgaacggcgc
     2221 ggcaccgctg ccctggagcc ttttctgcat gatctggcaa gagcgaaagg gtcgcagcga
     2281 ctaggaccaa agatgcgtgc gggccttgag gcagaactgg cgatgctggt gcaacaggga
     2341 aaggtagcga tcggcggggg cgacatacgg ctgctttgag ccccaggaac gcgtttcttg
     2401 cgtacactcg aaccagcacg agcccggccg cctac
//
LOCUS       DAOSIT010000358         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37445_length_2435_cov_8.062185, whole genome shotgun sequence.
ACCESSION   DAOSIT010000358 DAOSIT010000000
VERSION     DAOSIT010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37445_length_2435_cov_8.062185"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLL14_08680"
     CDS             <1..1150
                     /locus_tag="PLL14_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG04045.1"
                     /translation="RRLLFALLVLLLIAAGAWWATRPKPVAVVLKTIERGLVESTIAN
                     TRAGTVEACQRTRLSTISGGRIEVLAVKEGDRVSKGQLLLKLWNDDQQAQTAWVASQV
                     ATAYKRVSEACVVVDNAEREANRQSALRIQGFVSSAREDSARSDALARRAGCDAAKAD
                     LAQAEARVSLTRVEQGRTVLYAPFAGTIAKIVGEVGEYSTPSPPGVPTPPAIDLIDDS
                     CLYVKAPMDEVDAPRIAAGQAVRISLDALPRRSFPGKVRRVAAYVSAVEKQARTVDIE
                     VAFDDPAAPGKLLVGYSADVEVLLDIRENVIRIPSSALLEGGRVLVAAADGTLEERQV
                     KTGLANWEFTEVLEGLSTGEQVVTSLERAGVKAGASYSIDSKPATGGR"
     gene            1154..1858
                     /locus_tag="PLL14_08685"
     CDS             1154..1858
                     /locus_tag="PLL14_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG04046.1"
                     /translation="MGAALIELAGIERIFQLGDSEVHALRRLDVCIAAGEYVAVMGPS
                     GSGKSTLLNLLGLLDRPNAGIYRLAGRDVTTLSADEQARVRSERIGFVFQSFHLVPRL
                     TAAENIALPMILAGIAPAQRSARVTRALRDYGLENRADHRPDQLSGGQRQRVAIARAT
                     IMQPALILADEPTGNLDRATGEEVMRLLEELNHSGVTLIVVTHDTSLGARARRQLLMA
                     DGALESDRWRGNEAIP"
     gene            1861..>2435
                     /locus_tag="PLL14_08690"
     CDS             1861..>2435
                     /locus_tag="PLL14_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04047.1"
                     /translation="MRAPDLIRFARDAASGSPLRTVLLVLAMAIGVAAVVVLTALGDG
                     ARRYVLREFSSIGSNLVIVLPGRSQTGGFNPGNAITSTPRDLTVDDAQALLRASAVHR
                     VAPLAVGTSEISVGGKLREVMVAGTTAAFIDVRRLALAQGRFLPPGDWRRGASEAVIG
                     ARIRDEMFGREEALGQLVRIGDRRFRIVGVLA"
BASE COUNT          415 a          827 c          794 g          399 t
ORIGIN      
        1 ccgccgtctg ctcttcgcgc ttcttgtcct gctgctgatc gcggcgggcg cctggtgggc
       61 gacgcggccg aaaccggtcg ccgtcgtgtt gaagaccatc gaacgcggcc tggtcgagtc
      121 aaccatcgcc aacacccgcg ccggtacagt cgaagcctgt cagcgcacca gactgtcgac
      181 gatcagcggc ggccggatcg aggtcctggc ggtcaaggag ggcgatcggg tcagcaaggg
      241 ccaattgctg ctgaaactgt ggaacgacga ccagcaagcg cagaccgcat gggttgcatc
      301 acaggtggcc acggcttaca aacgcgtcag tgaagcctgt gtcgtggtgg acaatgccga
      361 gcgcgaggcc aatcgccaat cggcactgcg catccagggt tttgtctcca gcgccagaga
      421 agatagcgcg cgcagcgatg cattggccag gcgagcgggt tgcgacgccg ccaaggccga
      481 cctcgcacaa gccgaagcga gagtcagcct gactcgcgtc gagcaggggc ggacggtgct
      541 ctatgcgcca tttgccggca ccatcgccaa gatcgtcggt gaagtgggcg agtactcgac
      601 accgtcgccc cccggcgtcc cgacgccgcc ggcgatcgac ctgatcgacg actcctgtct
      661 ctacgtcaag gcaccgatgg atgaagtcga tgccccccgg atcgctgccg gacaggcggt
      721 gcgcatcagc ctcgacgcac taccgcgacg gtccttcccc ggcaaggtca ggcgtgtggc
      781 agcgtacgtc tcggcggtcg aaaagcaggc ccgcaccgtc gatatcgagg tcgccttcga
      841 cgacccggca gcgccgggca agctgctggt cggctacagc gcggatgtcg aagtgctcct
      901 cgacatccgc gagaacgtca tccggattcc cagctcggcg ctgctcgaag gcggacgcgt
      961 gctggtggct gccgccgacg gcacgctcga agaacgccag gtgaagaccg gattggccaa
     1021 ttgggaattc accgaagtgc tcgaaggctt gtcgaccggc gaacaggtgg tcacctcgct
     1081 cgaacgcgct ggcgtcaagg ccggtgcctc ctattcgatc gacagcaagc cggcaactgg
     1141 cggcagatag tcgatgggcg ctgcgctgat cgaactcgcc ggtattgagc ggatcttcca
     1201 gctcggtgac agcgaggtgc acgccttgcg ccgcctggac gtgtgcatcg ccgccgggga
     1261 atacgtagcc gtcatgggtc cgtcgggatc gggaaaatcc acgctgctca acctgctcgg
     1321 cctgctcgat cgccccaatg ccggcatcta ccgtctcgca ggccgtgacg tcaccaccct
     1381 gtcggcagac gaacaggcgc gcgtgcgcag cgagcgcatc ggcttcgtct ttcagagctt
     1441 tcatctggtg ccgcgcctga ccgctgccga gaacatcgcc ctgccaatga ttctcgccgg
     1501 catcgcgccg gcacagcgca gcgcacgcgt cacccgcgcc ctgcgggact atggactgga
     1561 gaaccgcgcc gaccatcgcc cggaccaact ctccggtggg cagcggcagc gggtcgcgat
     1621 cgcccgcgcg acgatcatgc agcccgcact gatccttgcc gacgagccta ccggcaacct
     1681 cgatcgcgcc accggcgaag aagtgatgcg tttgctcgaa gaactcaacc acagcggggt
     1741 caccttgatc gtcgtcaccc acgacaccag ccttggcgcc cgcgcccgcc gccagttgct
     1801 gatggccgac ggagcgctcg agagcgaccg ctggcgtggc aacgaggcta ttccataagc
     1861 atgcgcgctc ccgacctgat tcgcttcgct cgcgatgcgg ccagcggcag tcccttgcgc
     1921 accgtgctgc tggtactggc gatggccatc ggcgtcgctg ccgtggtggt gctgacggcg
     1981 cttggcgatg gcgcgcgccg ctatgtgctc cgggagtttt cgtcgatcgg cagcaacctg
     2041 gtaatcgtcc tgccaggccg ctcgcagacc ggtggcttca accccggcaa cgccatcacc
     2101 agcaccccgc gcgatctgac cgtcgatgat gcgcaggccc tgctgcgcgc cagcgccgtg
     2161 caccgcgtcg caccgctggc cgtcggcact tccgaaatca gcgttggcgg caagttgcgc
     2221 gaagtcatgg tcgccggcac caccgccgca ttcatcgacg tgcgtcgcct ggccctggcg
     2281 caagggcgtt tcctgccacc cggcgactgg cgccgcggcg catccgaagc ggtcatcggg
     2341 gccaggattc gcgatgaaat gttcggtcgc gaggaagctc tggggcaact ggtgcgtatc
     2401 ggtgaccgac gcttccggat tgtcggcgtc ctcgc
//
LOCUS       DAOSIT010000359         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37604_length_2427_cov_11.121417, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000359 DAOSIT010000000
VERSION     DAOSIT010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37604_length_2427_cov_11.121417"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1526
                     /gene="nifB"
                     /locus_tag="PLL14_08695"
     CDS             9..1526
                     /gene="nifB"
                     /locus_tag="PLL14_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOG04048.1"
                     /translation="MDLPVIESSPPAATGCSAGSCGSTAQQVDGLSDAIRARVQDHPC
                     YSEDAHHYFARMHVAVAPACNIQCHYCNRKYDCANESRPGVVSELLTPDQAVRKTLAV
                     AATIPQMTVLGIAGPGDPLANPQRTFETFRQLALKAPDIKLCVSTNGLVLPDHVDELA
                     RYNIDHVTITINCVDPDVGARIYPWIFWKNKRVFGREAAAILIEQQQKGLEMLTARGI
                     LVKVNSVLIPGVNDQHLPEVSKVVKAKGAFLHNVMPLIAEAEHGTFYGVMGQRSPKPA
                     ELKALQDACEGDMNMMRHCRQCRADAVGLLGEDRGAEFTLDRIDTMEIDYEAAMVVRA
                     ELRAQIAAELAEKRARAHPPAATATPQVISFQPHAAKPAPVPGRPVLMAVAARDGLVA
                     VHFGHAREFLVYEASASGARLVGHRKAESYCSGDESCGDAESVLEKTIRALADCEVVL
                     CSRIGYEPWGQLEAAGIQPNGEHAMQPIADAVMAVWNEMLAAGKLAVGPIVAKRA"
     gene            1537..1815
                     /locus_tag="PLL14_08700"
     CDS             1537..1815
                     /locus_tag="PLL14_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG04049.1"
                     /translation="MALEIIESCVNCWACAPLCPNQAIYEATPHFLIEADKCTECLGD
                     FAEAQCASICPIEGAIINELGEALNPPGSLTGIPPERWAAAQAEIAAR"
     gene            1818..2309
                     /locus_tag="PLL14_08705"
     CDS             1818..2309
                     /locus_tag="PLL14_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOG04050.1"
                     /translation="MADVIFYEKTGCAGNARQKALLIAAGHQVESRDLRQHFWSNLRL
                     LEFLADLPVAQWFNPSAAAIKSGEIVPDEFDQRTALAVLRNNPLLIRRPLLQVGDERR
                     VGFDAATIDAWIGLPDFPSGDLEACRRKPSSGRVYYVQSAGGGAFPCRFDDDPAHARR
                     CSP"
     gene            2287..>2427
                     /locus_tag="PLL14_08710"
     CDS             2287..>2427
                     /locus_tag="PLL14_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOG04051.1"
                     /translation="MPAAALPESAVSVAPGVHWIGAFDPDLRNFDIILRTANGTSYNA
                     YAV"
BASE COUNT          453 a          830 c          762 g          382 t
ORIGIN      
        1 agctcgccat ggatctgcca gtcatcgaat cctcaccgcc cgcagccacc ggctgtagcg
       61 ccggtagttg cggctccacg gcgcagcagg tggatggcct ctccgacgcc attcgcgcca
      121 gggtgcagga ccatccctgt tattcggaag acgcccacca ctacttcgcc cgcatgcacg
      181 tcgcggtggc gccggcctgc aacatccagt gccactactg caaccgcaag tacgactgcg
      241 ccaacgagtc acggccgggc gtcgtttccg aactgctgac accggatcag gcggtccgga
      301 aaacgctggc ggtggcggcg acgatcccgc agatgaccgt cctcggcatt gccggtcccg
      361 gcgatccgct ggccaatccg cagcgaacct ttgaaacctt ccggcaactg gcgttgaagg
      421 cgccggacat caagctctgc gtgtcgacca acggcctggt gctgcccgat cacgtcgatg
      481 aactggcgcg ttacaacatc gaccacgtca ccatcaccat caactgcgtg gaccccgacg
      541 tcggtgcccg catctatccc tggatattct ggaagaacaa gcgcgtcttt ggccgcgagg
      601 cggcggcgat cctcatcgag cagcagcaga agggcctgga aatgctcacc gcgcgcggca
      661 tcctggtgaa ggtcaactcg gtgctgatcc ccggcgtcaa tgaccagcac ctgccggaag
      721 tgtcgaaggt ggtcaaggcc aagggagcct ttctgcacaa cgtcatgccg ctgatcgccg
      781 aggccgaaca cggcactttc tacggcgtga tgggacaaag gagcccgaaa ccggcggaac
      841 tcaaggccct gcaggatgcc tgcgaaggcg acatgaacat gatgcgccat tgccgccagt
      901 gccgcgccga tgcggtcggc ctgctcggcg aggaccgcgg tgccgagttc accctcgaca
      961 ggatcgacac catggagatc gactacgaag cggcaatggt ggtacgcgcc gaactgcgcg
     1021 cgcagattgc cgccgagctg gccgaaaaac gcgccagggc acacccgccg gccgccaccg
     1081 caacgccaca ggtgatcagc ttccagccgc acgccgcgaa gccggcgcca gtacccggtc
     1141 gaccggtgtt gatggccgtc gccgccaggg acgggctggt cgccgtgcac ttcgggcacg
     1201 cccgcgagtt cctcgtatat gaagcctcag ccagcggcgc ccgcctggtc ggccaccgca
     1261 aggcggaatc ctactgttcg ggcgacgaaa gctgcggcga cgccgagtcg gtgctggaga
     1321 aaacgatcag ggcgctcgcc gactgcgaag tcgtgctctg ctcgcgcatc ggatacgaac
     1381 cctggggcca actggaggcg gccggcatcc agcccaacgg cgaacacgcc atgcaaccga
     1441 tcgcggatgc ggtcatggcg gtatggaacg agatgctggc ggcgggcaag ctggccgtcg
     1501 gaccaatcgt cgccaaacgc gcctgaggag gacaaggtgg ccctcgaaat catcgaaagc
     1561 tgcgttaact gctgggcatg cgctcccctc tgcccgaatc aggcgatcta cgaagcgaca
     1621 ccgcacttcc tgatcgaggc cgacaaatgt accgaatgcc tgggcgactt cgccgaggcg
     1681 cagtgcgcca gcatctgccc gatcgaaggg gcgatcatca acgaactcgg tgaagcgctc
     1741 aatccgccgg ggtcgctgac cggcatcccg cccgagcgct gggcggccgc gcaggccgag
     1801 attgcggcgc gctagccatg gccgacgtca ttttctacga gaagaccggc tgtgcgggca
     1861 acgcccgcca gaaagccctg ctgatcgccg ccggccatca ggtcgagagc cgcgacctgc
     1921 gccagcactt ctggagcaat ctgcgcctgc tggagttcct cgccgacctg ccggtcgcgc
     1981 agtggttcaa cccctcggca gcagcgatca agtccggtga aatcgtgccg gacgaattcg
     2041 accagcgtac cgcgttggcc gtgttgcgca acaatccact gctgattcgc cggccgctgc
     2101 tgcaagtcgg cgacgagcgg cgggtgggct tcgatgccgc gacgatcgac gcctggatcg
     2161 ggctgccgga cttcccgtcg ggtgaccttg aagcctgccg ccgcaaaccc tccagcggcc
     2221 gtgtctatta tgtgcaatca gccggcggcg gtgcttttcc ctgccgcttc gacgacgacc
     2281 cagcgcatgc ccgccgctgc tctccctgaa agcgcggtgt ctgtcgcccc cggtgtgcat
     2341 tggatcggcg ctttcgaccc ggacctgcgc aacttcgaca tcatcctgcg caccgccaac
     2401 ggcaccagct acaacgccta tgcggtg
//
LOCUS       DAOSIT010000360         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37789_length_2418_cov_9.057554, whole genome shotgun sequence.
ACCESSION   DAOSIT010000360 DAOSIT010000000
VERSION     DAOSIT010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37789_length_2418_cov_9.057554"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PLL14_08715"
     CDS             complement(<1..1178)
                     /locus_tag="PLL14_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04052.1"
                     /translation="MLKLALRNILRHKLRTAMTLAAIIFGVVGLILSGGFVEDIFLQL
                     AETTIHSQSGHLQIYKSGFFAQGSRAPEKYLIDEPDSIRGALERFPEIDDTMARVNFS
                     GLLNNGRTDLSIVGEGVEADKEARLGSHLRISAGRQLGKADAYGILVGHGVAASLKLI
                     PGERVTLLLNTAEGAMNSLEFEVVGVFQTFSKEFDARAVRIPLAAAQELLNTQGINRI
                     VLSLKKTEDTERVTRSLAAQLDGALYELKTWRQLNDFYQKTVDLYARQFGVLRLIVLI
                     MVFLGVANTINMSVFERTGEFGTMMALGNTRMTIFQLVLLENVLLGLIGASVGALLGV
                     AMAVLISTVGIVMPPPPNANIGYTALIRIVPSVVVMAFLVGLIATILAAIFPASRVAR
                     T"
     gene            complement(1210..>2418)
                     /locus_tag="PLL14_08720"
     CDS             complement(1210..>2418)
                     /locus_tag="PLL14_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04053.1"
                     /translation="LFLALRNVLRQKRRSAVAISAVGFGVVALILAAGFIEWIYQAMR
                     EDTIHSHLGHAQIVRPGYLDSGMADPFAYLLPAAPDELKTIESLPHVRTVTPRLAFSG
                     LISHGEATISFVADGVDPEKEELLSRTLLISKGEGLSSRDPQGIIIGDGLAANLGVTV
                     GDRVVLLANTASGGINAVEAHVRGLFSTVTKAYDDSALRLPLPAARQLLRVTGAHRWL
                     VLLDDTAHTESLVAELRQRLTGKKLEVVAWHQLADFYNKTVALFSKQVGVMKLIIAII
                     IVLSISNTLMMSVMERTGEIGTSMALGYSRQAILRLFVSEAALLGLFGGLLGILVGVG
                     LAHLISAIGIPMPPPPGMARGFTGEIHVTWRLTLDALGLAFGTTLLASVYPAWKASRM
                     EIVDALRHSR"
BASE COUNT          467 a          744 c          765 g          442 t
ORIGIN      
        1 gttcgggcta cccggctggc gggaaagatc gctgccagga tggtcgcgat cagaccgacc
       61 aggaaggcca ttaccaccac cgaaggcacg atgcgaatca gcgccgtata gccgatattg
      121 gcgttcggcg gcgggggcat gacgatcccg accgtcgaga tcagaactgc catggcgact
      181 cccagcaacg cgcccacgct cgccccaatg aggccgagca ggacgttttc gagcagcacc
      241 agttgaaaga tggtcattct ggtgttcccc agggccatca tcgtgccgaa ttcgccggtg
      301 cgctcgaaga cgctcatgtt gatggtgttg gccacgccga gaaagaccat gatcaggacg
      361 atcagccgca atacgccgaa ctgccgggcg taaaggtcga cggtcttctg gtagaagtca
      421 ttgagttgcc gccaggtctt caactcatag agcgcaccat cgagttgcgc ggcgaggctg
      481 cgggtgacgc gttcggtatc ctcggtcttc ttcaacgaga gcacgatgcg gttgatgccc
      541 tgggtattga gcagttcctg tgccgcagcc agcggaatcc gcaccgcgcg ggcgtcgaac
      601 tccttcgaga aagtctggaa gacgcccacc acctcgaact ccaggctgtt catcgcaccc
      661 tcggcggtat tcagcagcag cgtcacacgc tcgccgggta tcagcttcag gctggccgcc
      721 acgccgtggc cgacgaggat tccataagca tccgccttgc cgagctggcg tcctgcggag
      781 atcctgagat ggctgcccag cctggcctcc ttgtcagcct cgacgccttc cccgacgatc
      841 gacaggtccg tgcgcccgtt gttgagcagg cccgagaaat tgacccgcgc catggtgtcg
      901 tcgatttccg ggaagcgttc cagggcgccc ctgatactgt caggttcgtc gatcaggtac
      961 ttttccggtg cccgcgatcc ctgggcgaaa aaaccggact tgtagatctg caggtgtccc
     1021 gactgtgaat ggatggtcgt ctcggcgagt tgcaggaaga tgtcctcgac gaagccaccg
     1081 ctgagaatca gcccgaccac gccaaagatg atcgccgcca gggtcatcgc cgtcctgagt
     1141 ttgtgtcgga gaatgttgcg cagggcaagt ttcagcatgg gtcagtctcg ccggtcgaag
     1201 tgcggtggct cagcggctgt ggcgcagggc atcgacgatc tccatccgtg acgctttcca
     1261 tgccgggtag acgctcgcca gcagagtggt gccgaaggcc aggcccagtg cgtccagggt
     1321 cagccgccag gtcacatgga tttctccggt gaaaccgcgc gccataccgg gtgggggggg
     1381 catcggaatg ccgatggccg agatcaggtg cgccaggccc actccgacca ggatcccgag
     1441 caagccgccg aaaagtccga gcagagcggc ttcgctgacg aacagtcgga gaattgcctg
     1501 ccggctgtaa ccgagcgcca tcgacgtgcc gatttcgccg gtgcgctcca tgacgctcat
     1561 catcagggta ttcgagatgc tgagcacgat gatgatggcg atgatcagct tcatgacccc
     1621 gacctgtttc gagaacagtg cgacggtctt gttgtagaaa tccgccaact gatgccaggc
     1681 caccacttcg agttttttgc cggtcaaccg ctgccgcagt tcagcgacca gcgattccgt
     1741 gtgggcggtg tcgtcgagca gcaccagcca gcgatgcgcg ccggtaaccc gcagcagttg
     1801 ccgtgctgcc ggcaagggca gacgcaacgc cgaatcgtcg taggccttgg tgactgtcga
     1861 gaacaggcca cgtacgtgcg cctcgacggc gttgatgccg cccgaggcgg tgttggccag
     1921 caggacgacc cggtcgccca ccgtcacccc gagattggcc gccagtccgt cgccgatgat
     1981 gatgccctgc ggatccctgg acgacagtcc ttcgcctttc gagatcagca gcgtacggct
     2041 gagcagttcc tccttctcgg gatcgacgcc atcggcgacg aacgagatcg tcgcttcgcc
     2101 atggctgatc aagccgctga aggccagtcg cggggtgacg gtccggacat gcggcaggct
     2161 ttcgatggtt ttcagttcgt caggtgccgc gggcaggaga taggcgaaag gatcggccat
     2221 gcccgaatcc agatagcccg gacggacaat ctgcgcatgg cccagatgtg aatggatggt
     2281 gtcctcgcgc atcgcctgat agatccattc gatgaatccg gcggcgagga tcagcgcaac
     2341 gacgccgaag ccgaccgccg agatcgccac cgccgagcgc ctcttctggc gcaagacgtt
     2401 gcgcagcgct aggaaaag
//
LOCUS       DAOSIT010000361         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_37871_length_2415_cov_7.515678, whole genome shotgun sequence.
ACCESSION   DAOSIT010000361 DAOSIT010000000
VERSION     DAOSIT010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37871_length_2415_cov_7.515678"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLL14_08725"
     CDS             complement(<1..318)
                     /locus_tag="PLL14_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG04054.1"
                     /translation="MTLPALLGDLWGAILAYVFFYPVLMSLVWMIGGIGFYLRFERKP
                     HRHVDQPPLREHYPLIAVLVPCYNEEANVEETVDALMELRYPNFEVIAINDGSKDRTG
                     ELLN"
     gene            complement(315..1886)
                     /gene="pgaB"
                     /locus_tag="PLL14_08730"
     CDS             complement(315..1886)
                     /gene="pgaB"
                     /locus_tag="PLL14_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HOG04055.1"
                     /translation="MALLIRALLAAALCCLCTLPAAQDAPRKDTRDDNSRFIALSYHE
                     VLSDHEPLTPTAVRASDLAAQFAWLADNDYHPVSVDQILAARRGDEPLPANAVLLTFD
                     DGKKDVYSRVFPLLKLFRYPAVIALVGQWLDVPANALVDYDGQQVARSAFVTWQEVRE
                     MQASGLVEVASHAYSLHHGILANPQGNTQPAATTRLYADGRYEDDRAYAARLEAGLKN
                     VLGERLGKSPRIIVWPYGRCNLAAQEIAASLGMPIGLTLEDGHNDAHTPKAAWKRQLI
                     ENSPSLQAFAEMLRAAWAPDPARSVHIDPGQWHDLETGLSATLDRLLTLSPNIALVKP
                     TVVRDGREAVLFPTSRRPVAADVLNHIAWQTERRAGVPVFIDLPEDWLNEPELVGDLA
                     RHVNFAGLRIRAVPESDVAQRIRQVAGQWRWPLRLIHASGEPPPTQPGQPLPKGDLLA
                     VAAGESTMASLPRALADEVLFEFDPRLPAAEIARRMRELDAAGFRQFGLTGIPEIGMD
                     TIAPVLSLRSQPQLR"
     gene            complement(1874..>2415)
                     /locus_tag="PLL14_08735"
     CDS             complement(1874..>2415)
                     /locus_tag="PLL14_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04056.1"
                     /translation="TNQIALRAVADGVEASRVSVGADWRAHESRNFALALAASDFSDN
                     NRRTAVNAAWFERWSSGPRWMFETTLGADASHNTLSSPVNYFNPANDHSLWLTAAIEN
                     LSWRHYDHSFRQRLALTGGRYWQADYAAGAIEAIEYTHRWELDRNFSLRYGIGRSLRP
                     YDGVSEGRTFGTLVMLWRF"
BASE COUNT          408 a          724 c          843 g          440 t
ORIGIN      
        1 gttgagcagt tcgccggtac ggtccttgct gccgtcgttg atggcgatca cctcgaagtt
       61 ggggtaacgc aattccatca aggcgtcgac ggtttcttcg acgttcgctt cctcgttgta
      121 acagggcacc aggacagcga tcagcgggta gtgttcgcgc aacggcggtt ggtcaacatg
      181 acgatgcggc ttgcgctcga aacgcaggta aaagccgatg ccaccgatca tccagaccag
      241 cgacatcagt accggataga aaaagacgta ggcgaggatt gcgccccaga ggtcgccaag
      301 cagtgcgggc agggtcatcg tagctgcggc tgcgagcgca gcgagaggac tggtgcgatg
      361 gtatccatgc caatttccgg aatgccggtg agaccgaact gccggaaacc ggcggcgtcg
      421 agttcacgca ttcgccgcgc gatctcggct gccggcagac gcgggtcgaa ttcgaacagc
      481 acctcgtcgg caagtgctct tggcagggag gccatggttg attcaccggc cgccacggcc
      541 agcagatcgc ctttgggcag aggctgtcct ggctgcgtgg gcggtggttc cccgctcgcg
      601 tggatcagtc gcaatggcca gcgccattgc ccggcgacct gccggatccg ttgcgcgacg
      661 tcgctttccg gtactgccct gatgcgcaag ccggcgaagt tgacgtgccg ggcgaggtcg
      721 ccgaccagtt ccggttcgtt cagccagtct tcgggcagat cgatgaatac cggcacgccg
      781 gcgcgccgct cggtctgcca ggcgatgtga ttcagcacat cggcggcaac cggtcgccga
      841 ctggtcggaa agagcaccgc ctcccggccg tcacgcacga ccgttggctt gaccagggca
      901 atgttcgggc tcagggtcag caggcgatca agcgtcgccg agagcccggt ttcgagatcg
      961 tgccactggc cgggatcgat atgcacgctg cgtgccgggt cgggcgccca ggcggcgcgc
     1021 agcatttcgg caaaggcctg cagggagggg ctgttttcga tcaactgccg tttccatgcc
     1081 gccttcggcg tgtgcgcgtc gttgtggccg tcctcgaggg tcaggccaat cggcatgccg
     1141 aggctggcgg cgatttcctg cgcggccagg ttgcagcggc cgtagggcca gacgatgatg
     1201 cgcggcgatt ttccgagacg ctcgccgagc acgttcttca gtccggcctc aaggcgggcg
     1261 gcataggccc ggtcatcttc atagcggccg tccgcgtaga gtcgcgtcgt cgccgcgggc
     1321 tgggtgttgc cctgcggatt ggcgaggatg ccatggtgca ggctgtaggc gtgtgaggct
     1381 acctcgacca gccccgaggc ctgcatttcc ctgacctcct gccaggtgac gaaagcgctg
     1441 cgcgcgacct gctggccgtc gtaatcgacc agcgcgttgg ccgggacgtc cagccactga
     1501 ccgaccaggg cgatgacggc tgggtagcga aagagcttga gcagtggaaa gacacggctg
     1561 tagacgtcct tcttcccgtc gtcgaaggtt aacagcacgg cattcgcagg caagggttcg
     1621 tcgcccctgc gggcggcgag aatctggtcg acgctcaccg ggtggtagtc gttgtccgcc
     1681 agccaggcga attgcgcggc aaggtcgctg gcgcgtaccg cggttggcgt caagggctcg
     1741 tggtcactga gcacttcgtg gtagctcagc gcaatgaagc gggagttgtc gtcgcgtgtg
     1801 tccttgcgtg gagcgtcctg tgccgccggc agcgtgcaca ggcagcacag cgcagcggcc
     1861 agcagggcgc ggatcagaag cgccataaca tcaccagcgt gccaaatgtt cgtccttcgc
     1921 tgaccccgtc gtagggacgc aggctgcgtc cgatgccgta gcgaagcgag aagttgcggt
     1981 ccagttccca gcgatgggtg tattcgatcg cttcgatggc gcccgcggca tagtcagcct
     2041 gccagtagcg gccaccggtc agcgccagac gttgccggaa ggagtggtcg tagtgacgcc
     2101 aggacaggtt ttcgatggcc gcggtcagcc acagtgaatg gtcgttggcc gggttgaaat
     2161 agttgacggg gctgctcagt gtgttgtgcg aggcgtcggc gccaagcgtg gtctcgaaca
     2221 tccagcgcgg accgctggac cagcgttcga accaggcggc attgaccgca gtgcgccggt
     2281 tgttgtcgga gaaatcgctg gccgcgaggg ctagcgcgaa attgcgtgac tcgtgagcgc
     2341 gccagtcggc accgacactg acccggctgg cctcgacccc gtcggccacg gcgcgcagcg
     2401 caatctggtt ggtga
//
LOCUS       DAOSIT010000362         2406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38068_length_2406_cov_12.420247, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000362 DAOSIT010000000
VERSION     DAOSIT010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38068_length_2406_cov_12.420247"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLL14_08740"
     CDS             <1..516
                     /locus_tag="PLL14_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOG04057.1"
                     /translation="LESERGELAAISADLNAALATGQDELGIVLIQKKNALEASTKAL
                     EQEMEQARADAQEAKDSLIQVKAEIQKLKDEKDRMLAQMHSAQARLKIQNQLEGLSVD
                     AEVRALDNVREHIKNTVAEAKMGSELRDSDLDVKLAKLRQSSGAITARQQLEEMKRAR
                     NVQTDAAGKAM"
     gene            554..>2406
                     /locus_tag="PLL14_08745"
     CDS             554..>2406
                     /locus_tag="PLL14_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG04058.1"
                     /translation="MSPESGGRPALPAWAETLRQKYLAGEASVFVLYRNVFDRYQVGE
                     RYYTLLPFLSEVLLKDNKQRIFELSIDRGVRVIQGGGIDEKAELYRHLEGKGLAGIFE
                     SLERQMREQRSSALLIPYAGTIFPAAELHFLSLEERGAFTALHRWSLDEEFADRDNVV
                     ILVSESLADLSPALLTHPRVLAIEVPMPDRAGRQAAIRHYAPAMKAAEAEQLADQTAG
                     LRLLQLASIVASEAPQGMSHAQRRTLITGLLQGSPQAAERAEKLAAITAGMTPAEIRQ
                     LVDPTRALPDDGDPASEMMAVVRQRKRELIEKECAGLIEFIEPRHGLDSVGGNAHIKG
                     ELLDIARLIVSGERARAPMGLLAVGPMGAGKTFVIKAFLKEAGLSGIALKNFRSKWVG
                     STEANLERVLATVKAMGPIALVIDEGDRSFGRQGDDSDGGTSSRVIARLKEFMSDPEN
                     RGQVLFILMTNRPDKLDTDIKRPGRLDRKIPFFYAESAGERAAVVSAVFQRYRVPVDL
                     SENDLLAACEPLEGYSNADLEAVALLAAEFAERALRTDAAPGGADPRAREAPSPVVSP
                     EILARAIEDFMPPQETTMVRYMEMLAVAETSRRSLLPQRFRSLSARAVQEQL"
BASE COUNT          453 a          783 c          797 g          373 t
ORIGIN      
        1 ctggaaagcg aacgtggcga actggcggcg atctccgccg acctcaatgc ggcgctggcc
       61 accggtcagg atgaactcgg catcgtgctg attcagaaga agaacgcgct tgaggccagc
      121 accaaggcgc tggagcagga aatggagcag gcgcgtgccg acgcgcagga ggcgaaggat
      181 tcgctgatcc aggtcaaggc cgagattcag aagctgaagg acgagaagga ccgcatgctg
      241 gcgcagatgc acagcgcgca ggcgcgcctg aagattcaga accagctcga aggcctgtcg
      301 gtcgatgccg aagtccgggc gctcgacaac gtccgcgagc acatcaagaa caccgtcgcc
      361 gaggccaaga tgggcagcga actgcgcgac tccgatctcg acgtcaagct cgccaagctg
      421 cggcagagca gcggcgcgat caccgcccgc cagcaacttg aggaaatgaa acgcgcgcgc
      481 aacgtgcaga ccgatgccgc cggcaaggcg atgtgatgcg cgacgtgtcg cgctgccgga
      541 ctggcgggtg tcggtgagtc ccgagagcgg cggccggcca gccctgccgg cctgggccga
      601 gaccctgcgc cagaagtacc tggccggcga ggcgtcggta ttcgtgctct atcgcaacgt
      661 gttcgaccgc tatcaggtcg gcgaacgcta ttacacgctg ttgcccttcc tcagcgaggt
      721 gctgctcaag gacaacaagc agcgcatctt cgaactcagc atcgatcgcg gcgtacgggt
      781 gatccagggt ggcggcatcg acgagaaggc ggaactctac cgccatctcg aaggcaaggg
      841 tctcgccggg atcttcgagt cgctcgaacg tcagatgcgc gagcagcgct ccagcgcgct
      901 gctgattccc tatgcgggga ccattttccc cgccgccgaa ctgcactttc tctcgcttga
      961 ggagcgtggc gcgttcaccg cattgcaccg ctggtcgctc gacgaagagt ttgccgaccg
     1021 tgacaatgtc gtcatcctgg tcagcgaatc gctggccgat ctcagcccgg cactgctgac
     1081 gcatccgcgc gtgctggcca tcgaggtgcc gatgccggac cgcgccggcc gacaggcggc
     1141 gatccgtcac tacgcgccgg cgatgaaggc tgcggaagcc gagcaactcg ccgaccagac
     1201 cgcgggtctg cgcctgctgc aactggcgag catcgtcgcc agcgaagcgc cgcagggcat
     1261 gagtcacgcg cagcggcgga cattgatcac cggtttgctg caaggcagcc cgcaggctgc
     1321 cgagcgtgcc gaaaaactgg ccgcgatcac tgccggcatg acccctgccg agatccggca
     1381 actcgtcgat ccgacgcgcg cgctgcccga tgacggcgac ccggcgagcg aaatgatggc
     1441 ggtcgtgcgc cagcgcaagc gcgagttgat cgaaaaggag tgcgccggtc tgatcgagtt
     1501 catcgaaccg cggcacggcc tggacagcgt cggcggtaac gcgcacatca agggtgagct
     1561 actcgacatc gcgcgcctga tcgtcagtgg cgagcgcgcg cgcgcgccga tgggactgct
     1621 cgcggtgggg ccgatgggcg ccggcaagac cttcgtgatc aaggccttcc tcaaggaggc
     1681 ggggttgtcc ggcatcgccc tgaaaaactt ccgctcgaag tgggtcggct cgaccgaggc
     1741 caatctcgaa cgcgtcctgg cgaccgtcaa ggccatgggg ccgatcgctc tggtcatcga
     1801 cgaaggcgac cgcagcttcg gcaggcaggg tgacgattcc gacgggggaa cgagttcgcg
     1861 cgtcatcgct cgtctgaagg aattcatgtc tgatcccgag aatcgcggcc aggtgctgtt
     1921 catcctgatg accaaccgcc ccgacaagct cgataccgac atcaaacgcc ccggtcgcct
     1981 tgatcgcaag attcccttct tctacgccga gagcgctggc gaacgcgccg ccgtcgtcag
     2041 cgccgttttt cagcgctacc gggtgcccgt cgatctttcg gaaaacgacc tgctcgccgc
     2101 ctgcgaaccc ctcgaaggct attcgaacgc cgacctcgaa gccgttgcct tgctcgccgc
     2161 cgagtttgcc gagcgtgcgc tacggaccga cgcggccccg ggcggtgccg acccgcgggc
     2221 gagggaggcg ccatcgccgg tcgtcagtcc ggagattctc gcgcgggcca tcgaggactt
     2281 catgccaccg caggagacga ccatggtgcg ctatatggaa atgctcgcgg tggccgagac
     2341 ctcgcgacgc tcgctgctgc cacaacgctt ccgatcgctg tccgcgcgcg cggtgcagga
     2401 acagct
//
LOCUS       DAOSIT010000363         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38120_length_2403_cov_6.479983, whole genome shotgun sequence.
ACCESSION   DAOSIT010000363 DAOSIT010000000
VERSION     DAOSIT010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38120_length_2403_cov_6.479983"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..78)
                     /locus_tag="PLL14_08750"
     tRNA            complement(4..78)
                     /locus_tag="PLL14_08750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Thr,seq:ggt)
     gene            complement(120..193)
                     /locus_tag="PLL14_08755"
     tRNA            complement(120..193)
                     /locus_tag="PLL14_08755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Gly,seq:tcc)
     gene            complement(245..329)
                     /locus_tag="PLL14_08760"
     tRNA            complement(245..329)
                     /locus_tag="PLL14_08760"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Tyr,seq:gta)
     gene            801..>2403
                     /locus_tag="PLL14_08765"
     CDS             801..>2403
                     /locus_tag="PLL14_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOG04059.1"
                     /translation="MGDLSTTLDSRYTATSGRVFLSGTQALVRLPMLQRLRDEAAGLN
                     TAGFVSGYRGSPLGGLDQALWKAGRHLEAQRIVFQPAVNEDLAATAVWGTQQLNLFSG
                     AKHQGVFAMWYGKGPGVDRCGDVFRHANAAGSARYGGVLVIAGDDHAAKSSTLPHQTE
                     HIFKAVMMPVLYPANVQEYLDYGLHGWAMSRYSGCWVVMKALADTVETSASVSIDPLS
                     PCIRLPDDFEMPANDPHGLNIRWPDPPLQQEARLLNHKLYAALAYCRANQLNRVVIDS
                     TDPASPARLGIITAGKAYLDVRQALGELGIDDELAAQIGIRLYKVGMVWPLEADGVRR
                     FAEGLEEILVVEEKRQLLEYQLKEELYNWREDVRPRVVGKFDEKGEWSLLPTGSGHLT
                     HGDWLLPAAGELSVTQVARALAARIGRFFTSPAIEARLALLEAKQKSLAPVQLPAAGP
                     AAIQRTPYFCSGCPHNTSTRVPAGSRAIAGIGCHYMVTWMDRSTATFTHMGGEGVTWV
                     GQAPFSSEKHIFANLGDGTYFHSGLL"
BASE COUNT          464 a          778 c          726 g          435 t
ORIGIN      
        1 cggtggtgcc catggacaga attgaactgt cgacctctcc cttaccaagg gagtgctcta
       61 ccgctgagct acatgggcgt cgcttgagaa gcttgaaacc attttgcggc cgcacaacct
      121 ggagcgggtg aagggaatcg aaccctcgtc ataagcttgg aaggcttcag ctctaccatt
      181 gagctacacc cgcaaaacca ttacccgaag cacgattagt cagcatcacc aacagcggcg
      241 tttttggtgg agggagaagg attcgaacct tcgaaggcag agccgtcaga tttacagtct
      301 gatcccgttg gccacttggg tatccctccg gctaagaacc cacaattctg acgtccaatg
      361 aaaggactgt caaatgattt ttcaagccga cgccgattat tccttgggag cagtagatca
      421 acctactggt tccggagctt attgcaggtc gacggcgtaa aatcgcacca ttaccgaccc
      481 accagccgga ctggacacag tgctcgcaga ccatgaacat tcaccgtaca ctgccgctgc
      541 cggacgactt tgaagccacc ctgaaaaacc gctttggctg ccgcttcgca cagggagagt
      601 cggtatgtgc ccaacagggg cgcattgctt cggtgcatcg acccgcgcct cctgcttggc
      661 cttgaacttg gtcgcccccg tcgccagcgc gcaacctgcg cccgggcaag cggtctgagc
      721 agtggcgggc tcgccggcgg cagggaagca cgcccgcctg ggcctcgatc accagcagcc
      781 acagtcaggg aggagaaacg atgggcgatc tctcgacaac gctcgacagt cgctatacgg
      841 cgacctcggg acgcgttttt ctgagtggta cccaagccct cgttcgtctg ccaatgctgc
      901 agcgcctgcg cgacgaggct gccgggctga acaccgccgg ttttgtttcc gggtaccgcg
      961 gcagccccct cggtgggctc gatcaggcgc tctggaaggc cggtcggcac ctcgaagccc
     1021 agcgcatcgt cttccagccg gcagtcaacg aggacctcgc cgcaaccgcc gtctggggca
     1081 cgcagcagct caatctcttt tccggcgcca agcaccaggg ggtgttcgcc atgtggtacg
     1141 gcaagggccc cggtgtcgac cgctgcggag acgttttccg gcatgccaat gctgccggat
     1201 cggcaagata tggcggcgtc ctggtcatcg ccggcgacga ccacgcggcc aagtcctcga
     1261 ccctgccgca ccagaccgag cacatcttca aggcggtgat gatgccggtc ctctatccgg
     1321 ccaacgtcca ggagtatctc gattatgggc tgcatggctg ggcgatgagt cgctactcgg
     1381 gatgctgggt ggtgatgaag gccctggccg acacggtcga aacctcggcc tcggtctcga
     1441 tcgacccgct ttctccatgc atccggctgc cggatgactt cgagatgcca gcgaacgacc
     1501 cgcacggcct caacatccgc tggccggacc caccgctaca gcaggaagcc cgcctgctca
     1561 accacaagct gtatgcggcg ctcgcctact gccgcgccaa ccagctcaac cgcgtggtga
     1621 tcgacagcac cgacccagcc tccccggccc gcctgggaat catcaccgcc ggcaaggcct
     1681 atcttgatgt ccgccaggcg ctcggcgaac tggggatcga tgatgaactg gcggcacaga
     1741 tcggcattcg cctgtacaag gtgggcatgg tctggccgct cgaagcggac ggcgtgcgtc
     1801 gtttcgccga gggtctcgaa gagatcctgg tggtcgagga aaagcgacaa ctcctcgaat
     1861 accagctcaa ggaggagctc tacaactggc gcgaggatgt gcgtccgcgc gtcgtgggaa
     1921 agtttgacga aaagggcgaa tggtcgctgc tgccgaccgg tagtgggcat ctgacgcatg
     1981 gcgactggct gttgccggct gccggcgaac tgtcggtaac ccaggtggca cgggcgctgg
     2041 cggcacgcat cggccgcttc ttcacttcgc cggcaatcga ggcacgcttg gcgctgctcg
     2101 aagccaagca gaaaagcctc gcgccggtgc agcttcccgc ggccgggccg gcagccatcc
     2161 agcgcacgcc ctacttctgt tcgggttgcc cacacaacac ctcgacacgc gtgcccgcgg
     2221 gttcccgcgc catcgccggt atcggctgtc actacatggt cacctggatg gatcgcagca
     2281 ccgccacctt cacgcatatg ggcggcgaag gcgtgacctg ggtcggccag gcgccgttca
     2341 gcagtgaaaa acacatcttc gccaacctgg gcgacggtac ctatttccat tccgggctgc
     2401 tgg
//
LOCUS       DAOSIT010000364         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38146_length_2402_cov_7.119301, whole genome shotgun sequence.
ACCESSION   DAOSIT010000364 DAOSIT010000000
VERSION     DAOSIT010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38146_length_2402_cov_7.119301"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLL14_08770"
     CDS             <1..389
                     /locus_tag="PLL14_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOG04060.1"
                     /translation="AGTQNMADIRGLIKVSPTVGWGLMLGSLAILGMPPFGVFASEFL
                     ILTTAMREQPWATPFLLLALGVAFAAVFSRVQPMVFGETSVRPLAHPPALLPVFAHLG
                     LGLMLGLYIPPYLDDWYRQAARMMGG"
     gene            393..1964
                     /locus_tag="PLL14_08775"
     CDS             393..1964
                     /locus_tag="PLL14_08775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOG04061.1"
                     /translation="MLIADLSLDVERLPVALPMWRATVRAETWRALAQTLSTAGGRLL
                     SLWGSDRRRGAAGGLVVSAAYVLADGLLWLDLPLGERNPGYPDVAPFFPCAARMQRAA
                     ADLLGVSAEGVADRRGWLDHGAWPVQGFPLRREGGAATPLPAEPLGAPEYAFVRVEGD
                     GVHEIPVGPVHAGIIEPGHFRFAVVGEKVLRLEQRLGYTHKGVEKRFTDLPPLQAHRL
                     AGRISGDSTVAYAWACCMALESLAGGEISERACSLRALLLERERVANHLGDLGALGND
                     AAFAFGLAQFSRLREDWLRLSQEAFGHRLMMDCVVPGGVASDIDAVMADRLSRQCDVI
                     EREVLELRQVYDGHAGLQDRFMTTGRVTPELAAQLGLTGLAGRASGQATDLRRDQHWP
                     PYDRLAVRIATRREGDVAARVGVRFDEVLESLRLLRLLRLLLARPGDGDTRSALSWPE
                     QAAFGAGWVEGWRGEVFVAIEWDGERGAIRRCHVHDPSWQNWPVLEHAVIGNIVPDFP
                     LINKSFNLSYSGHDL"
     gene            1964..>2402
                     /locus_tag="PLL14_08780"
     CDS             1964..>2402
                     /locus_tag="PLL14_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HOG04062.1"
                     /translation="MWRILRQIARHGICSEAAPACSDALRVQGAAIQDELLAILGQAL
                     TIRQVDAGSCNGCELEINALNNPYTNLEGLGIKFVASPRHADLLLVTGPVTRNMAEAL
                     RRTYDATPAPKLVVAVGDCGCDGGIFGESYATCGRVANVIPVDV"
BASE COUNT          338 a          830 c          822 g          412 t
ORIGIN      
        1 aggccgggac gcagaacatg gccgacatcc gcggcctgat caaggtcagc ccgaccgtcg
       61 gctggggctt gatgctcggc tcgctggcga tcctcggcat gccgcccttc ggcgtcttcg
      121 ccagcgaatt cctgattctc accaccgcca tgcgcgagca gccgtgggcc acccccttcc
      181 tgctgctggc gctgggcgtc gccttcgccg ccgtgttcag ccgcgtgcag ccgatggtct
      241 tcggcgaaac gtcggtcagg cccctggcgc accccccggc actgctgccg gtgtttgcgc
      301 accttggtct gggtctcatg ctgggcctgt atatcccgcc gtacctggac gactggtatc
      361 gtcaggcggc gcgaatgatg ggaggttgag gggtgctgat cgccgacctg agtctggatg
      421 tcgaacgcct gccggtcgcg ctgccgatgt ggcgtgccac ggtccgcgcc gagacctggc
      481 gggcgcttgc tcagacgctg tccacggccg gcggacggct gttgtccctg tggggctccg
      541 atcgccgccg cggcgccgcc ggcgggctgg tcgtctcggc ggcctatgtt cttgccgacg
      601 gcctgctctg gctggatctg ccgcttggcg aacggaaccc cggctatccg gacgtggcgc
      661 cgttttttcc ctgcgcggcg cgcatgcagc gcgcggcagc ggacttgctg ggggtgagcg
      721 ccgaaggggt cgccgaccgg cgcggctggc tcgaccacgg cgcctggccg gtgcaggggt
      781 ttccgctgcg ccgggaaggg ggcgcggcca ccccactgcc ggcagaaccc ttgggcgcac
      841 cagagtatgc cttcgtgcgt gtcgaaggcg acggtgtgca cgagatcccc gtcggaccgg
      901 tacacgccgg catcatcgaa ccggggcact tccgctttgc ggtagttggc gagaaagtgc
      961 tgcgcctgga gcaacgcctg gggtacacgc acaagggcgt cgaaaagcgc ttcaccgatc
     1021 tgccgccact gcaggcgcat cgcctggccg gacggatttc cggcgattcg acggtggctt
     1081 acgcctgggc ctgttgcatg gctctggaat cgctcgccgg tggcgagatc tccgagcggg
     1141 cgtgctcgtt gcgggcgctg ctgctcgagc gcgaacgcgt ggccaaccat ctcggcgacc
     1201 tcggcgcact cggcaacgac gccgccttcg ccttcggact ggcgcagttc tcgcgactca
     1261 gggaagactg gctgcgcctg tcgcaggagg ccttcggaca tcgcctgatg atggactgcg
     1321 tggtgcccgg cggcgtcgcg tcggatattg acgctgtgat ggccgatcgt ctgtcacgcc
     1381 agtgcgacgt gatcgaacgc gaagtgctgg aattgcgcca ggtgtacgac gggcatgccg
     1441 ggttgcagga ccgcttcatg accaccgggc gggtgacgcc ggaactggcc gcccaactcg
     1501 gactcaccgg cctcgccggc cgcgccagcg gccaggccac cgacctgcgc cgtgaccagc
     1561 actggccacc gtacgatcgc ctggccgtgc gcatcgccac gcgtcgcgaa ggcgacgtcg
     1621 cggcgcgcgt cggcgtgcgt tttgacgagg tgctcgaatc cctgcgcctg ctgcgcctgc
     1681 tgcgcctgct gctcgccagg cctggcgacg gcgacacgcg cagcgcactg tcctggcccg
     1741 agcaggccgc gtttggcgcc ggctgggtcg aaggctggcg cggcgaagtc ttcgtcgcca
     1801 tcgaatggga cggcgagcgt ggcgccatcc gtcgctgcca cgtccacgat ccctcctggc
     1861 agaactggcc ggtgctcgaa cacgcggtga tcggcaacat cgtccccgac tttccgctga
     1921 tcaacaagtc cttcaacctc agctactcgg gacacgacct ctgatgtggc gcatcctcag
     1981 acagatcgca cgccacggca tttgcagcga ggccgcgccc gcgtgcagcg acgccctgcg
     2041 cgtgcaaggc gcagcgatcc aggacgaact gctggccatt ctcggccagg cgctgaccat
     2101 tcgccaggtc gatgccggtt cctgcaacgg ctgcgagctc gaaatcaacg ccctgaacaa
     2161 tccctacacc aatctcgaag gcctgggcat caagttcgtc gccagcccgc gccacgccga
     2221 cctgctgctg gtcaccggac cggtgacgcg caacatggca gaggccttgc gccggaccta
     2281 tgacgctacc ccggcgccga aactggtcgt cgccgtcggc gactgcggct gcgacggcgg
     2341 catcttcggc gagagctacg cgacttgcgg tcgcgtggcc aatgtcatcc cggtcgatgt
     2401 cg
//
LOCUS       DAOSIT010000365         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38222_length_2398_cov_5.329492, whole genome shotgun sequence.
ACCESSION   DAOSIT010000365 DAOSIT010000000
VERSION     DAOSIT010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38222_length_2398_cov_5.329492"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLL14_08785"
     CDS             complement(<1..181)
                     /locus_tag="PLL14_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG04063.1"
                     /translation="MLNAEKISRSTVPQALRAKQWRPHFRRGQRIYAGEAHGGAWRVL
                     SGSVRLDREEPNGEQS"
     gene            complement(200..2398)
                     /locus_tag="PLL14_08790"
     CDS             complement(200..2398)
                     /locus_tag="PLL14_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOG04064.1"
                     /translation="MDAAGIVPYRAASSDTARLLERLPGVSLYGAGGVSSLPAIHGLA
                     DDRIRIKVDGMDLIASCPNHMNPPLSYLDPTNVGTLKVYAGITPVSVGGDSIAGTIIA
                     ETPAPVFAAPGQNVLTTGQLGGFYRSNGNAWGANIAATAATENISLTYTGSSARADNY
                     KAGGNFKTTTATGRIGHTLPLDEVGSTAYESLNQMLSFALKGGNHLVEAKFGFQHIPE
                     QLYPNQRMDMLDNEQKTFNLRYLGQYDWGLFEARAYYEKVDPHFMDFGPDKRYWYGPG
                     APPAGSGGDNAVNGSPCSPISGTRMVNGAMAGCAAGMPMYTRSSNTGVNVKAEMNVDT
                     RNLLRIGGLYQHYTLNDWWPPSGAMMWPGTFDNINDGSRDRAGLFGEWESHLDRQWLT
                     LLGVRYERVTTDAGNVRGYDPTTNGMGMMVNDQKKDAAAFNSQNHERADNNWDLTALA
                     RYTPDANSDIEFGVARKVRSPNLYERYTWSTWSMAAVMNNFVGDGNGYFGNIHLKPEQ
                     AHTVSASFDLHAADRRWEFRATPYYTRVTDYIDAIRCPAGASCTPANRTTTNQFVVLQ
                     YANQSAELYGLDVSGHMPLASTGMGDFGLTGSLTYTHGKNLDTGDGLYNIMPLNGKLA
                     LTHALGGWKGVVEIVAVAAKDDLSDVRNETRTAGYGLTNLRGSYTWKQVRLDLGVDNL
                     FNKFYSLPLGGAYVGQGTTMALNPGSGNPAWGTAVPGMGRSIYAGMNLRF"
BASE COUNT          387 a          715 c          795 g          501 t
ORIGIN      
        1 agctttgttc gccgttgggt tcctcgcggt cgaggcggac cgagccgctc agcacgcgcc
       61 aggcaccgcc gtgggcctcg ccggcgtaga tgcgctgccc gcgcctgaag tggggtcgcc
      121 actgtttcgc gcgcagcgcc tgtgggacgg tgctgcgtga aatcttttcg gcattcaaca
      181 tttctgcttc ctctcgctcc tagaatcgta gattcatgcc ggcatagatc gagcgtccca
      241 tgccgggaac cgcggttccc catgcggggt tgccgctccc agggttaagc gccatggtgg
      301 ttccttgacc gacgtaggcg ccgccaagcg gcagcgaata gaacttgttg aacaggttgt
      361 cgactccaag gtcgagtcgc acctgcttcc aggtgtagct gccgcgcaga ttggtcagcc
      421 cgtagcccgc cgtcctggtt tcgttgcgca cgtcggagag atcgtctttc gccgcaaccg
      481 cgacaatctc gaccacacct ttccagccac ccagcgcgtg cgtcagcgcc agcttgccgt
      541 tgagcggcat gatgttgtag aggccgtcgc cggtgtcaag attcttgccg tgggtgtagg
      601 tgagcgagcc ggtgaggcca aaatcaccca tcccggtcga ggcgagcggc atgtgcccgg
      661 aaacatcgag gccgtaaagc tcggccgact ggttggcata ctggaggaca acgaactgat
      721 tggtcgtcgt gcggttggct ggcgtgcagg atgcgcctgc cgggcagcga atggcatcga
      781 tgtagtcggt gacccgtgtg tagtagggcg tggcgcgaaa ttcccagcga cggtccgccg
      841 catgcaggtc gaaggaggcg gaaacggtgt gcgcctgttc cggcttgaga tggatattgc
      901 cgaagtagcc gttaccgtcg ccgacgaagt tgttcatcac ggcagccatc gaccaggtcg
      961 accaggtgta gcgctcatac aggttcggcg atcgtacctt gcgcgcgacg ccgaattcga
     1021 tgtcgctgtt ggcatccggc gtatagcggg caagcgctgt caggtcccag ttgttgtcgg
     1081 cgcgctcgtg attttgcgaa ttgaacgcgg cggcgtcctt cttctgatcg ttcaccatca
     1141 tgcccatgcc gttggtggtc gggtcatagc cccgaacatt gccggcatcc gtcgtgaccc
     1201 gttcgtaacg cacaccgagc agggtcagcc attgcctgtc gaggtgcgac tcccactcgc
     1261 cgaacaagcc tgcccggtcg cgcgagccgt cgttgatgtt gtcgaacgtg ccgggccaca
     1321 tcatcgcgcc ggatggtggc caccagtcgt tgagggtgta gtgctgatag aggccaccga
     1381 tgcggagcag gttccgcgta tcgacattca tctccgcctt gacgttcacc ccggtgttgc
     1441 tgctcctggt atacatcggc atgccggccg cgcatccggc catcgcgccg ttcaccatgc
     1501 gggttccgga aatcggggaa catggcgaac cgttgaccgc gttgtcacct ccggaacctg
     1561 ccggtggcgc tccagggccg taccagtacc gcttgtcggg gccgaagtcc atgaaatgcg
     1621 ggtcgacttt ctcgtaatag gcccgcgctt cgaacagccc ccagtcgtat tgaccgagat
     1681 agcgcaggtt gaatgtcttc tgctcgttgt cgagcatgtc catgcgctgg ttcggataaa
     1741 gttgttcggg gatgtgctgg aatccgaact tggcctcgac gagatggttg ccgcctttca
     1801 gtgcgaagct cagcatctgg ttcaggctct cgtaggccgt cgagccgact tcgtcgagcg
     1861 gcagcgtgtg cccgatgcgg ccggtggccg tggtcgtctt gaagttgccg ccggccttgt
     1921 agttgtcggc ccgggcactc gaaccggtat aggtcaggct gatgttttcg gtggcggcag
     1981 tagccgcgat gttggcgccc caggcgttgc cgttgctgcg gtagaagccg ccgagctggc
     2041 ccgtagtcag gacattctgg cccggcgcag cgaacaccgg cgccggcgtc tcggcgatga
     2101 tcgtgccggc gatgctgtcg ccaccgacgc tgaccggcgt gattccggca tagaccttga
     2161 gtgtgccgac attggtcgga tcgaggtagg atagcggcgg gttcatgtgg ttggggcagg
     2221 aagcgatcag gtccatgcca tcgaccttga tccggatgcg gtcatcagcc aggccgtgaa
     2281 ttgccggcag gctggatacc cctccggcac cgtagaggct gactcccggc aggcgttcga
     2341 gcaggcgggc ggtgtcgctg ctggccgcac ggtagggcac gatgcctgcc gcatcgat
//
LOCUS       DAOSIT010000366         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38293_length_2394_cov_8.795212, whole genome shotgun sequence.
ACCESSION   DAOSIT010000366 DAOSIT010000000
VERSION     DAOSIT010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38293_length_2394_cov_8.795212"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1354)
                     /locus_tag="PLL14_08795"
     CDS             complement(74..1354)
                     /locus_tag="PLL14_08795"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG04065.1"
                     /translation="MALIVQKFGGTSVASTERIKNVAKRVARWRAQGHDIILVPSAMA
                     GETNRLIGLAKEISPSPDPRELDVIASTGEQVTIALLAMAMHAEGVKAKSFTGSQVGV
                     LTDSTFTKARILKIDQERIRKALGDGNVVVVAGFQGADEDGNITTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRLDAITFEEMLEMASLGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEDEGEGTLITVEEKGKMEQPIISGIAFNRDEAKLTVLGVPDRPG
                     IAYQILGPIADAKIDVDMIIQNIGRDGTTDFSFTVNRSDYSKSLEILQTVKEKIGARD
                     ITGDNTTCKVSAVGVGMRSHPGVASRMFGALAKESINLQMISTSEIKISVVIEEKYLE
                     LAVRVLHKAFGLEKPPKSKLGLTGRGETDIITPS"
     gene            1598..1903
                     /locus_tag="PLL14_08800"
     CDS             1598..1903
                     /locus_tag="PLL14_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOG04066.1"
                     /translation="MSTVKVVERVDPREIRRKLGLNQQQFWSKIGVTQSGGSRYESGR
                     NMPKPVRELLRLVHVEQIDIQRLKREDFEVVEYLKAEDPELFKTLRKAARSKLKKPV"
     gene            complement(1978..>2394)
                     /locus_tag="PLL14_08805"
     CDS             complement(1978..>2394)
                     /locus_tag="PLL14_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG04067.1"
                     /translation="AGLAGSEPRSLAKVASGGELSRISLAIQVVASQAARVPTLIFDE
                     VDVGIGGGVAEVVGLLLRELGAERQVLCVTHLPQVAARANWQWQVSKVAVADQLRSHV
                     AMLTGEARVEEIARMLGGVEITAITRQHAREMLKLS"
BASE COUNT          481 a          695 c          701 g          517 t
ORIGIN      
        1 gaacccttga tgcaggttta tgcccgcatg ctcccttagc aggggagtgc cttcgacctc
       61 tcggccacgt ctcctaggag ggggtgatga tatcggtttc gccgcggccg gtcaaaccca
      121 acttcgactt tggcggtttt tcgaggccga aagccttgtg caggacgcgc acggcaagtt
      181 ccagatactt ctcttcgatg actaccgaga tcttgatttc ggatgtcgag atcatctgca
      241 ggttgatgct ttccttggcc agcgcgccga acatccggct cgcgacgccc gggtgggagc
      301 gcatcccgac accaacggcg gagactttgc aggtcgtgtt gtcgccggtg atgtcgcgtg
      361 cgccaatctt ttccttgact gtctgcagga tctcgaggga cttgctgtag tcacttcggt
      421 tgacggtgaa tgaaaagtca gtggtgccgt cacggccaat attctggatg atcatgtcga
      481 cgtcgatttt cgcatcggcg atcggcccca ggatctggta ggcaattccc gggcgatcgg
      541 gaacgcccag aacggtgagt ttggcttcat cgcgattgaa tgcaatcccg gaaatgatcg
      601 gttgttccat cttccctttt tcctcaacag taatcagtgt gccttcgcct tcgtcctcga
      661 agctggacag cacgcgaagc ttgactttgt atttgccggc gaactcgacc gatcgaatct
      721 ggagaacctt ggagccaagg ctggccattt ccagcatctc ttcaaaagtg atggcgtcga
      781 gtctgcgcgc ttccggcacg acgcgtgggt cggtggtata aacgccgtcc acgtccgtgt
      841 agatttggca ctcgtcggcc ttcagcgcgg ccgcaagcgc tacggccgaa gtgtctgagc
      901 caccacggcc cagcgtggtg atattgccgt cctcgtcggc gccttgaaag ccggcgacga
      961 cgacgacatt gccatcgccc aaggccttgc gaattcgttc ctggtcgatc ttcagaatgc
     1021 gtgccttggt aaaggtgcta tcggtcaaca cgccgacctg tgaaccggta aagctcttgg
     1081 ccttgacccc ctcggcatgc atggccatgg ccagcagggc gatggtcacc tgctctccgg
     1141 tcgaggcgat cacgtcgagt tcgcgggggt ctggggaagg cgaaatttcc ttggccaggc
     1201 cgatcaggcg attggtttca ccggccatgg ccgacggtac gaggatgatg tcgtggccct
     1261 gagctcgcca gcgggcgacg cgctttgcca cattcttgat gcgctccgtc gatgcgaccg
     1321 aagtgccgcc aaacttctga actatcagtg ccattccaga tccagttgaa aaccgaaaaa
     1381 gcctgtattc taaagcattg cctgatgtta cggcaaagga acgccgcccc ggatgagccg
     1441 atcggcatta gccagatgtt ctattagaat gccacttgca attctggatg aatcgcttat
     1501 agtgtgccgt gcattaccag agtcataggg tgtggatctc tggcaggaag tcggcttctt
     1561 gctggttcat ttgaggcaat tgtaagaaag gacagccatg agcaccgtca aagtcgttga
     1621 gagagtcgat ccacgagaaa tacgccgcaa gctgggtttg aatcagcaac agttctggtc
     1681 gaagattggc gtaacgcaaa gtggcggttc ccgctatgaa agcggccgca acatgcccaa
     1741 gccggtgcgt gaactgctgc ggctcgtgca tgtggagcag atcgacattc agcgcctgaa
     1801 acgcgaagac ttcgaggtcg tcgagtacct gaaggcagaa gaccccgaac tcttcaagac
     1861 cctccggaaa gcggcccgga gcaagctcaa gaagcccgtt tgattcgcgt cggtttgggc
     1921 gcgcagaagc cgtctcgggt accggcttta cacgcgcgct gtccggccgc gtactgatca
     1981 ggacagtttc aacatctccc ttgcatgctg gcgggtaatc gccgtgatct cgacacctcc
     2041 gagcatgcgg gcgatttctt cgacccgggc ttccccggta agcatggcga catggctgcg
     2101 aagctgatcg gcgaccgcga ccttgctcac ctgccactgc cagttggcac gcgccgccac
     2161 ctggggtagg tgggtgacac aaagaacctg ccgttcggct cccaactcgc gcagcaaaag
     2221 accgacgacc tcggcgactc cgccgccgat accgacatcc acttcgtcaa agatcaacgt
     2281 cgggactctc gctgcctgac tggcgacgac ctgaatggcg agactgatac gcgacagttc
     2341 gccacctgaa gccaccttgg ccagagaccg tggctcgctg ccggccagac cggc
//
LOCUS       DAOSIT010000367         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38371_length_2390_cov_8.992291, whole genome shotgun sequence.
ACCESSION   DAOSIT010000367 DAOSIT010000000
VERSION     DAOSIT010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38371_length_2390_cov_8.992291"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLL14_08810"
     CDS             complement(<1..689)
                     /locus_tag="PLL14_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOG04068.1"
                     /translation="MDKVARRQVALWLLLCSAMVFAILVVGGVTRLTHSGLSIVEWQP
                     IVGVIPPLNQQEWEDTFEKYKLTPEYQQVNHRMALAEFKGIFYWEYWHRVLGRLVGVV
                     FLLPFLYFWLRRKLAPALVPKLLGIFVLGGLQGAMGWYMVKSGLVDDPRVSHYRLTAH
                     LSLAFLIFVAMFWLALGLLAERVRTTHDVQLRQLQRVAFWLALLVGYMVVSGGLVAGI
                     RAGKAYNTFPL"
     gene            986..1135
                     /locus_tag="PLL14_08815"
     CDS             986..1135
                     /locus_tag="PLL14_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04069.1"
                     /translation="MADHNDDDLPFMQKLLDNHFLLLFLGVASPGLLYILWGIIDIMN
                     TPVAR"
     gene            1167..1706
                     /locus_tag="PLL14_08820"
     CDS             1167..1706
                     /locus_tag="PLL14_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HOG04070.1"
                     /translation="MSAILPPSDRLWWKQPIDKVEWAWIGIAFVWGMIMFVMMIYWHI
                     YGKQNLSNEAYKTSPEMFAAKAEKFIAENTVRTETEQQIPVVKVPAGGDGYLIARLWA
                     WYPILELEKGKTYKIHLSSMDYNHGFSLQPVNINIQVIPGYEHVVKMTPTKEGTYAIV
                     CNEYCGIGHHSMLGRIYVK"
     gene            1723..>2390
                     /locus_tag="PLL14_08825"
     CDS             1723..>2390
                     /locus_tag="PLL14_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOG04071.1"
                     /translation="MATTYRTCPVSGLQFEDQAEKLMRWNAVAGVLWLLVGGITALLV
                     ILTRWPAIKLLPADQFYLILTAHGIDMLILWILFFEMAVLYFASSTLLRCRLATPKIA
                     WLGFWLMVIGGITANIAIFQGESSVMFTSYVPMQAAPHFYLGIILVAVGALLGVAVFF
                     GTLVVAKKDRTYQGSLPLVTFGALTAAIIATFTILAGAGILIPTFLWSVGLIKEIDAQ
                     LYRMV"
BASE COUNT          517 a          747 c          667 g          459 t
ORIGIN      
        1 atcagcggaa aggtattgta ggccttgccg gcgcgtatgc cggcaaccag gccgccactg
       61 accaccatat agcccacgag taacgccagc cagaaagcga cgcgctgcaa ctgccgcaac
      121 tgcacatcgt gggtggtacg cacgcgctcg gccagcaagc ccagcgccag ccagaacatc
      181 gcgacaaaga tcaggaatgc cagcgacaga tgcgcggtca ggcggtagtg gctgacacga
      241 ggatcgtcga ccagtccgct cttgaccatg taccagccca ttgccccctg cagaccgccc
      301 agcacgaaga tgcccagcaa cttcggtacc agcgcagggg cgagtttgcg tcgcagccag
      361 aaatagagaa agggcaggag aaacacgacg ccgaccaggc gtcccagcac gcgatgccag
      421 tactcccagt agaagatgcc cttgaactcg gcaagtgcca tccgatggtt cacctgctgg
      481 tattccggcg tcagcttgta tttttcgaac gtatcctccc attcctgctg gttcaacggc
      541 gggatgacgc cgacgatcgg ttgccactcg acgatcgaca ggccggaatg cgtcagccgc
      601 gtgacaccac cgacaaccag aatggcaaag accatggcac tgcaaagcag cagccaaagc
      661 gcaacttgtc ggcgagcgac tttatccatg acatgtcacc ggggaacgga aaaaggggaa
      721 aaggcgcgaa ttatatgccc tttgtcgcgc tccttgaggg ccgcaccggc tgcgaccttt
      781 agccgcaggt ctggcgctgc gacactgatg ccagcaggct ttgccggtgc ccgcgtggtc
      841 tcccgttctt gacgtgaatc aaatcaatga acacctgcct gcaggtattc tgtgggcaat
      901 tgtgccccaa aagcccaaga aattcgactt atctgcaacc cggcatgcgc cgcgcgcgcc
      961 aatcagtgat ggaggataca aaaacatggc cgaccacaac gatgacgacc ttccgttcat
     1021 gcaaaagctg ctggacaacc acttcctgct gctgtttctc ggcgtagcct cccctgggct
     1081 gctctatatc ctgtggggca tcatcgacat catgaatacg ccggtcgcca gataggccaa
     1141 tccgttcaca tcagggagag tcaactatga gtgcaattct gccgccgtcc gatcggctct
     1201 ggtggaagca acccatcgac aaggtcgaat gggcgtggat cgggatcgcc ttcgtctggg
     1261 ggatgatcat gttcgtgatg atgatctatt ggcatatcta cggtaagcag aacctgtcca
     1321 acgaggccta caagaccagc ccggagatgt tcgccgcgaa ggccgagaag ttcatcgccg
     1381 agaacaccgt gcgcaccgaa accgagcagc agatcccggt ggtcaaggta cccgccggcg
     1441 gtgacggcta cctgattgcc cgcctgtggg cctggtaccc gattctcgaa ctcgaaaaag
     1501 gtaagaccta caagattcat ctgtcttcca tggactacaa ccatggcttc tcgctgcaac
     1561 cggtaaacat caacatccag gtgatccccg gctatgaaca tgtcgtcaag atgacgccga
     1621 ccaaggaagg cacttacgcc atcgtctgca acgagtactg cggcatcggc caccactcga
     1681 tgctcggccg tatctacgtg aaataagagg gggagtaaat tgatggcaac cacctaccgt
     1741 acctgtcccg tctccggact gcaattcgag gaccaggccg agaagctgat gcgctggaac
     1801 gccgttgccg gcgtactctg gctgctggtc ggcggcatca ccgcgctgct ggtcatcctc
     1861 acccgctggc cggcgatcaa gctgctgccg gccgaccagt tctacctgat tcttaccgcc
     1921 cacggcatcg acatgctgat cctgtggatc ctgttcttcg aaatggccgt tctctacttt
     1981 gcctcatcga cgctgctccg ctgccggctg gccacaccga agattgcgtg gctcggattc
     2041 tggctgatgg tgattggcgg catcacggcc aacatcgcca ttttccaggg cgaatcgagc
     2101 gtgatgttca cctcgtacgt cccgatgcag gccgcgccgc acttctacct gggaatcatc
     2161 ctggtggccg tcggcgcact gctcggcgtc gccgtcttct tcggtaccct ggtggtcgcc
     2221 aagaaggacc ggacctacca gggctcgctg ccgctggtga cattcggcgc gctgaccgca
     2281 gcgatcatcg ccacgttcac catcctcgcc ggcgccggca tcctcatccc gaccttcctg
     2341 tggtcggtcg gcctgatcaa ggaaatcgat gcgcagttgt accgcatggt
//
LOCUS       DAOSIT010000368         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38516_length_2382_cov_9.534594, whole genome shotgun sequence.
ACCESSION   DAOSIT010000368 DAOSIT010000000
VERSION     DAOSIT010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38516_length_2382_cov_9.534594"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLL14_08830"
     CDS             <1..499
                     /locus_tag="PLL14_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04072.1"
                     /translation="GESGMNREELRDQLLAPVLQWTALGRQSSELMTGSLAVIHQRSN
                     RLLRGATLREAHDWAEMGAMTHEKVTVPLESAVAMATALQNEAQQFVTQTAEASLAVT
                     GSAMALFGSRSADEFGARQMALGQALANVAWCWYQLYGRVATVAEQGMRPILRQVKDN
                     AQRLS"
     gene            640..1731
                     /locus_tag="PLL14_08835"
     CDS             640..1731
                     /locus_tag="PLL14_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04073.1"
                     /translation="MKPSIAKLFITAAFCLSLGPVAAADLDLAGDIKAQAAKLVKVEN
                     PEVLPTIKRVVITSFMVDFVSELKYSKSLNGLEAMIGADSDVSIKLVGSSNEQFQSIA
                     DRFYDQTVELLKARGIEVVSQDELTVLPEFAELAASGVTPLPSEQDAKAGKGLFFSAH
                     GLPLHLIDETQFISRISFGKKQEDDFLTFGARFSGGFAAAQAQQIESRIARKLDATIL
                     KVRLTVLGGQLTPDAGFWTSGKVTTRAAASFVNFVTRYAFIDVEGNKARVSLQETVST
                     DEIGELVNTTSGGTKTVDTAKNVALVALNVASFAARIGGMRTGYTGGFASTSEYECRV
                     QAEPFEKTLLSHHDVIVRMFGERMQNPGQ"
     gene            complement(1757..2365)
                     /locus_tag="PLL14_08840"
     CDS             complement(1757..2365)
                     /locus_tag="PLL14_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOG04074.1"
                     /translation="MNRNDFEILDSEVCYQGFYRLERLRLRHRLFAGGMSPAIVREVI
                     EKSDVAAVLLYDPQRDAVVMIEQFRIGARDNPCGPWLLEIVAGLIEAGETPADVARRE
                     AMEEAGCALIDLLPISTFYTSPAKTSQRTHLYLGRVDCSSTGGVHGLAHEGEDIRVVC
                     LPATQAIALAESGDIDSAWPLIALFWFARHREGLRVRWSAGE"
BASE COUNT          469 a          704 c          761 g          448 t
ORIGIN      
        1 aggagaatca ggcatgaatc gagaagaact ccgtgaccag ttgctggcgc cggtcctgca
       61 atggacggcg ctgggccgac agagtagcga gttgatgacc ggctccctgg cggtaatcca
      121 tcagcgcagc aatcgcctgc tgcgtggcgc taccctgcgc gaagcgcacg actgggccga
      181 aatgggtgcc atgacccacg agaaggtcac tgttccgctg gagtcggctg tggccatggc
      241 cacagcgctg cagaacgaag cgcagcagtt cgtgacccag accgcagagg cgtcattggc
      301 cgtcaccggc agcgcgatgg ccctgtttgg cagtcgcagt gccgacgagt ttggcgcccg
      361 gcagatggca ctgggccagg cattggcgaa tgtcgcgtgg tgttggtatc aactctacgg
      421 acgggttgcc acggtggcgg agcagggcat gcggccgatt cttcgtcagg tgaaggataa
      481 cgcgcaacgg ctgagttaag cctggcggtc gcccgatgga tggggtgaca gggtctgtcg
      541 tttgatcgac cttcaccggg ccagcgtgcg gaattgccga tgtatcacac tccggattgc
      601 tagaatcacg cttttttcaa ccctgacgag atgcacgaca tgaaaccatc aatcgcgaag
      661 ctgttcatca ccgctgcctt ttgcctgagc cttgggcctg ttgctgcagc cgatctcgat
      721 cttgccggcg acatcaaggc gcaagcggca aaactcgtca aggtcgaaaa cccggaagtc
      781 ctgccgacga tcaagcgtgt ggtcattacc agcttcatgg tcgatttcgt cagcgagttg
      841 aagtacagca agtcgctgaa tggtctcgaa gccatgatcg gtgcggattc cgatgtgtcg
      901 atcaagctcg ttggcagcag taacgagcag ttccagtcga tcgccgaccg tttttacgac
      961 cagaccgtgg aattgctcaa ggcgcgtggc atcgaggtcg tcagtcagga tgaattgacc
     1021 gtcttgcccg agtttgccga actcgctgcc tccggggtca cgccgttgcc tagtgagcag
     1081 gacgccaagg ccggcaaggg tctgttcttc agtgcgcacg gactgccact gcacctgatc
     1141 gacgaaaccc agttcatcag caggatttct ttcggcaaga agcaggagga tgatttcctg
     1201 accttcggtg cgcgtttctc cggcggcttt gcggcagcgc aggcgcagca gatcgaaagc
     1261 aggatcgcca ggaagctcga cgcgacgatc ctgaaggtgc gcctgaccgt gctgggtggc
     1321 caactcactc ctgacgcggg tttctggacc agtggcaagg tcacgacgcg agctgctgcg
     1381 agtttcgtga acttcgtcac ccgctatgcg ttcattgatg tggaaggcaa caaggcgcgg
     1441 gtgtcgctcc aggagacggt gtctaccgat gaaatcggcg aactggtgaa taccaccagc
     1501 ggcggtacca agaccgtcga tacggcgaag aacgtggcgc tggtggcact caacgtggca
     1561 agttttgccg cccgcatcgg tggcatgcgg accggctaca ccggcggctt cgcctcgacc
     1621 agcgagtacg agtgtcgtgt tcaggcggaa cccttcgaaa agaccctcct gagccaccac
     1681 gacgtgattg tccggatgtt cggcgagagg atgcaaaatc ccgggcagtg aatagcgtgg
     1741 tggcgccctg cgccacctac tcgccggccg accaacgcac gcgcaaccct tcgcggtggc
     1801 gcgcaaacca gaagagggca atcagtggcc aggccgagtc gatatctccc gactcggcca
     1861 aggcgatcgc ctgcgtggcc ggcaggcaca cgacacggat atcctctccc tcgtgggcca
     1921 ggccgtgaac gccgccggtg ctggagcagt cgacgcgacc caggtagagg tgggtgcgct
     1981 gggaggtctt cgccgggctg gtgtaaaaag tgctgatcgg gagcaggtcg atcagcgcgc
     2041 aacccgcctc ttccatcgcc tcgcgtcgtg ccacatccgc cggggtttcg ccggcttcga
     2101 tgagcccggc gacgatctcg agcagccagg gaccgcaggg gttgtcgcgg gcgccaatcc
     2161 ggaactgttc gatcatcacc accgcatcac gctgcgggtc atagagcagg acggcggcga
     2221 catcgctctt ctcgatgacc tcgcgcacga tcgctggact catgccgccg gcgaacaggc
     2281 gatgacgcag tcgcaggcgc tcaagtcggt agaacccctg gtagcagacc tcgctgtcga
     2341 gtatctcgaa atcgttcctg ttcatgccga cctccgttga ct
//
LOCUS       DAOSIT010000369         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38624_length_2376_cov_6.627747, whole genome shotgun sequence.
ACCESSION   DAOSIT010000369 DAOSIT010000000
VERSION     DAOSIT010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38624_length_2376_cov_6.627747"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLL14_08845"
     CDS             complement(<1..284)
                     /locus_tag="PLL14_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOG04075.1"
                     /translation="MNGRNGTQRYGSLSIGLHWMMMLLFVAVYACIELRGYFPKGSDP
                     REALKTWHFMLGLSVLALALLRVLVHLRSTVPPIVPDPPGWQKLAARLMHL"
     gene            503..2191
                     /locus_tag="PLL14_08850"
     CDS             503..2191
                     /locus_tag="PLL14_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG04076.1"
                     /translation="MPDRIWLQHYPAGIPADIDADRYPSIPGLLDEIVGKYGGRPAFH
                     NLGRTLSYAELDKLSRDFATFLQGLPGMAKGERVAIMSPNLLQYPVALFGILRAGMIV
                     VNVNPLYTPKELEHQLQDSGARAIVVVENFAATLQQVWRKTALQHLITTQVGDLLPIP
                     KRWIVNFVVRTVKKMVPAWRLDGSLTFLEALARGAAARFTPVAIAASDLAFLQYTGGT
                     TGVSKGAMLSHRNLLANLEQTGIWISGSFEAGREIVIAPLPMYHIFCLTSTLSFMTWG
                     SLSVLISNPRDLPALVAELGRWKWSVLSGVNTLFNGLLHTPGFDALDFSSLKVVIGGG
                     AAVQRAVAEKWKQVTGCYITEAYGLTEASPGVSASPLDTPWNGMIGLPFPSTEVTLRN
                     EDFAALPPWTGDDDVEKHTGEICVRGPQVMLGYWGKPAETAAVLRDGWLTTGDIGHMN
                     QDGFITITDRKKDMILVSGFNVYPSEIEGVLASHPGVLECGAIGVPDARTGEAVKVVI
                     VRKDPQLSSEEVIRHCKAQLAAYKTPRQIEFRDALPKSPIGKILRRELRSPPQP"
BASE COUNT          431 a          816 c          745 g          384 t
ORIGIN      
        1 aggtgcatca gccgcgcggc gagcttttgc caccccggcg gatccggcac gatcggcggc
       61 accgtggacc gcaggtgtac cagcacgcgc agcaaggcga gggccaggac ggacaaaccg
      121 agcatgaagt gccaggtctt cagtgcctcg cgcggatcgc tgcctttcgg gaagtagccg
      181 cgcaactcaa tgcaggcgta aactgccaca aacagcagca tcatcatcca atgcagcccg
      241 atcgacagcg atccgtaacg ttgcgtgcca ttgcgcccgt tcatttgcgt gctccgtgaa
      301 gtgaaaaccg aatgctactg aaaagcgccg ctgccgcggt catttttcga caagcccagg
      361 ccaccatgat ggcggctgcg cgtcatcact tctgcgcgcc accgcgctta cgctcctccc
      421 ggaatgccgc ggcgtctggt gcgcctgcgt accgacggca tcccccggag cagcaacaat
      481 catgagacaa gaggagaaga cgatgcccga ccgaatctgg ctgcaacatt acccggctgg
      541 cattcccgcc gacatcgacg ccgaccgtta cccgtcgatt ccggggctgc tcgacgaaat
      601 cgtcggcaag tatggcggcc ggccggcctt ccacaatctc ggccgcacgc tgagctacgc
      661 cgagctcgac aagctgtcgc gcgacttcgc caccttcctg cagggcttgc ccggcatggc
      721 caagggcgag cgcgtggcga tcatgtcgcc aaacctgctg caatacccgg tggcgctgtt
      781 cggcatcctg cgggcgggca tgatcgtggt caacgtcaat ccgctgtaca ccccgaagga
      841 actcgaacac cagttgcagg actccggcgc cagggccatc gtcgtcgtcg agaacttcgc
      901 ggcgacgctg cagcaggtgt ggaggaagac cgcgctgcag cacctgatca ccacgcaggt
      961 cggcgatctg ttgccgatcc cgaagcgctg gatcgtcaac ttcgtggtca ggacggtgaa
     1021 gaagatggtt cccgcctggc gtctcgacgg ctccctgacc ttccttgagg cgctcgcccg
     1081 cggcgccgcg gcgcgcttca cgccggtcgc catcgcggcc tccgatctcg ccttcctgca
     1141 atacaccggc ggcaccaccg gcgtctccaa gggcgcgatg ctcagccacc gcaacctgct
     1201 cgccaacctc gaacagaccg gcatatggat ctccggcagc ttcgaggccg ggcgcgagat
     1261 cgtcatcgcg ccgctgccga tgtaccacat cttctgcctg acctcgacgc tgtcgttcat
     1321 gacctggggc agtctcagcg tgctgatcag caacccgcgc gacctgccgg cgctggtcgc
     1381 ggagctcggg cgctggaaat ggagcgtcct gagcggggtg aacaccctgt tcaacggcct
     1441 gctgcacacg ccgggattcg acgcgctcga cttctcgtcg ctgaaggtgg tgatcggcgg
     1501 cggcgcggcg gtgcaaagag cggtcgccga gaagtggaag caggtcaccg ggtgctacat
     1561 caccgaagcc tacggtctca ccgaagcctc gccgggggtc agcgcctcac cgctggatac
     1621 gccatggaac ggcatgatcg gcctgccgtt tccgtccacc gaggtgaccc tgcgcaacga
     1681 agacttcgcg gccctgccgc cgtggaccgg cgatgacgac gtcgaaaagc acaccggcga
     1741 gatttgcgtg cgcggcccgc aggtgatgct cggctactgg ggcaagcctg cggagaccgc
     1801 ggcggtgctg cgcgacggct ggctgaccac cggcgacatc gggcacatga atcaggacgg
     1861 cttcatcacc atcaccgacc gcaagaagga catgatcctg gtttccggtt tcaacgtcta
     1921 tccgagcgaa atcgaaggcg tgctggcgtc gcacccgggt gttctcgaat gcggcgccat
     1981 cggcgtgccc gacgcgcgta ccggcgaggc ggtgaaggtg gtgatcgtca ggaaggatcc
     2041 gcaactgagc agcgaggagg tgatccggca ctgcaaggcg cagcttgccg cctacaagac
     2101 gccgcgccag atcgagttcc gcgacgccct gccgaaatcg ccgatcggca agatcctgcg
     2161 ccgcgaactg cgtagcccgc cgcagccgta gcgcccgccg ccggtaattt ttcctcgtct
     2221 ccggagatcc tgccatgagt gcatccagcc catccccgcc gccgtcgtcg ccgggccgcc
     2281 tcgccgcccg ccccggaacc ggccagccga ccggctggct gccaagccga tccgttacgg
     2341 ctacatgaac ggcatcgcgc tgaccgtgct gatcag
//
LOCUS       DAOSIT010000370         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38812_length_2369_cov_8.479689, whole genome shotgun sequence.
ACCESSION   DAOSIT010000370 DAOSIT010000000
VERSION     DAOSIT010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38812_length_2369_cov_8.479689"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..634)
                     /locus_tag="PLL14_08855"
     CDS             complement(116..634)
                     /locus_tag="PLL14_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HOG04077.1"
                     /translation="MPKLVRCVWTLLMVVYAAGTAAAALREADEKPAEEIEVKFPASP
                     QEENLIPFVVSATTDNQFMIDGESLNLSGNGVLRFTLVIVSPSGARNVSYEGMNCSTG
                     ERRLYAFGRPDGTWSKARNDQWTRIIENSLNRHHAALFSEYFCPVGVTLRDADDARRA
                     LRYGGHSSTMQR"
     gene            complement(688..1233)
                     /locus_tag="PLL14_08860"
     CDS             complement(688..1233)
                     /locus_tag="PLL14_08860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HOG04078.1"
                     /translation="MLDREGYRPNVGIILCNARNEVFWGKRIREHSWQFPQGGIKRGE
                     TPEQAMYRELHEEIGLLPEHVFVLGRTKDWLRYDVPTHWVRREWRGSYRGQKQIWFLL
                     RLVGRDCDVSLRACDHPEFDAWRWNNYWVSLDAVIEFKRRVYEQALNELARFLNADQR
                     RGRSPPAHEQFSATPDIAGKE"
     gene            1463..>2369
                     /gene="proS"
                     /locus_tag="PLL14_08865"
     CDS             1463..>2369
                     /gene="proS"
                     /locus_tag="PLL14_08865"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG04079.1"
                     /translation="MRTSRFFISTLKEAPSEAEVVSHQLMLRAGMIKRLAGGIYTWMP
                     IGLRVLRKVEQIVREEMNRAGAIELSMPAVQPAELWQESGRWDKYGPELLRCKDRHQR
                     DFVIGPTHEEVITDVVRREVRSYRQLPLHFYQVQVKFRDEIRPRFGIMRGREFLMKDG
                     YSFHSSFADLQREYRNMFDTYTRIFNRLGLKFRAVAADTGSIGGTGSHEFHVLADSGE
                     DALAYCPDSGFAANVELAEALAPSTERAAATTVLQKVATPGRVKCEDVAEFLGIELTR
                     MVKAIAIVSSSSAGTLERFALILLRG"
BASE COUNT          461 a          701 c          716 g          491 t
ORIGIN      
        1 gctcgcggcg ggtgcgaaaa gcgtacgcag ggccttgtcg aactcgatga ggaagcggtc
       61 ggtaagcatg tgctgctttc ttggtgctgc gctatcggag ccactggaca gggtcctagc
      121 gctgcatggt cgatgaatgt ccgccgtaac ggagagcacg ccgagcgtca tcggcatcgc
      181 gaagcgtcac cccaacgggg cagaaatact cgctaaagag cgctgcatgg tggcgattga
      241 gcgagttctc gatgatccgc gtccactggt cgttgcgtgc tttcgaccaa gtgccgtccg
      301 ggcggccaaa tgcataaagc cgacgctccc cggtggagca gttcatgccc tcgtaactca
      361 cattgcgcgc gccggagggg ctgacaatca ccagcgtaaa gcgcagcacg ccgttgccgc
      421 tcaaattgag cgactctccg tcgatcatga actggttgtc ggttgtcgca ctgactacaa
      481 acgggatcag gttttcctcc tgcggagacg ccgggaattt cacctcgatc tcctcggccg
      541 gcttctcgtc cgcttcacgg agggctgccg ctgccgtacc tgcagcataa accaccatca
      601 acaaggtcca aacgcagcgt accagtttcg gcaacggcag gctccgccgg ggtgcggtgc
      661 gaaacggcgg cgaattcgga agtcgaatca ttccttgcct gcgatatcag gagttgcgga
      721 gaactgttcg tgggctggtg ggctgcgccc gcgacgctga tcggcattga gaaaacgggc
      781 caactcattc aaggcctgct cgtacacccg ccgcttgaac tcgatgaccg cgtcaagcga
      841 aacccagtag ttgttccagc gccaggcgtc aaactcagga tggtcgcagg cacgcaacga
      901 gacgtcacag tcacgcccga caagccgcag cagaaaccag atctgcttct gccctcggta
      961 gctgccacgc cattcgcgtc ttacccagtg cgttggcacg tcgtaacgca accagtcttt
     1021 cgttcggccg agaacgaata cgtgctcggg tagcagaccg atttcttcat gtaactcccg
     1081 gtacatcgcc tgctcgggcg tttcgccccg cttgatgcca ccctgcggaa actgccatga
     1141 atgctctcgg attcgcttgc cccagaagac ctcgttcctg gcgttacaaa gaatgatgcc
     1201 gacgttcggg cgatagcctt cacggtcaag cattatttaa aacctgcaaa ttgattgatt
     1261 ggacgcattt ttccacattt caacagcctt ggaaagcaaa ccgtgaccgg ggtgcgggtg
     1321 gtggttcgct ccactcgccg ggccgtttcc ccacggctgc atccgctgcg ccgacgaggg
     1381 ttggttcggt atgttattgg gggtcgcagt ttgaccctct gtctagtaag attgtgcctt
     1441 cgccgtccgt cagttcattc ccatgcgcac ctccaggttt ttcatttcca cactcaaaga
     1501 agcaccgtct gaggctgagg tggtcagtca ccagctcatg ctccgcgccg ggatgatcaa
     1561 acggctggct ggcggaatct acacctggat gccgatcggg ctgcgggtgc tgcgcaaggt
     1621 cgagcagatc gtccgcgagg agatgaatcg ggccggggcc atcgagttgt cgatgccggc
     1681 cgtgcagccg gcggaactat ggcaggagtc cgggcgctgg gacaagtatg gtcccgagtt
     1741 gttgcggtgc aaggatcggc atcagcgcga tttcgtgatc ggtccgacgc acgaggaggt
     1801 gattaccgat gtcgtgcgcc gggaggtcag gagctaccgt cagttgccgc tgcactttta
     1861 ccaggtgcag gtgaagttcc gcgacgagat ccgaccccgt ttcggtatca tgcgcggccg
     1921 cgaattcctg atgaaggatg gctactcgtt tcacagcagc ttcgcagacc tgcaacgcga
     1981 ataccgcaac atgttcgaca cgtatacgcg gatcttcaat cgtctagggc tgaaattccg
     2041 tgccgtagcc gccgataccg gttcgattgg cggaacggga tcgcacgagt tccatgtgct
     2101 ggcggattcc ggcgaggatg ccctggccta ttgccccgat tccggctttg cggccaacgt
     2161 cgaactcgcg gaggctctgg ccccatcaac agaacgcgcg gccgcgacca cggtcttgca
     2221 gaaggtggcc acacccggaa gggtcaagtg cgaggatgtc gccgaattcc tcggcatcga
     2281 actgacccgg atggtgaagg cgatcgccat cgtgtcaagt tcctccgcgg gcacccttga
     2341 acggtttgct ttgatcctgc tgcgcggcg
//
LOCUS       DAOSIT010000371         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38857_length_2367_cov_10.959775, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000371 DAOSIT010000000
VERSION     DAOSIT010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38857_length_2367_cov_10.959775"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLL14_08870"
     CDS             <1..522
                     /locus_tag="PLL14_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04080.1"
                     /translation="DIGDLLRELLTLYRETLFDSKGIAVETVLPGQAIRIACDRDSIK
                     QILLNLWKNASEALSRGQRIRLSLSDDVVHQGRRFAELRIEDNGPGMGEAAIDALHRP
                     AAGRIGGARGIGLSIVGTLTQRLGIPVTCRSKPGLGTMISLLLPTPTAAESRASDSNT
                     RHQTISSPDTSQE"
     gene            525..>2367
                     /locus_tag="PLL14_08875"
     CDS             525..>2367
                     /locus_tag="PLL14_08875"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG04081.1"
                     /translation="MKAIPQVDFLPHVPDSHKESSCNILIVDDEQRIRETYRQLLSAP
                     ERSIEDCGTGSEAMRRLEGRDIDVLILDLNLPDINGLEIMEWMLRRHVPTAVVVFSGD
                     ASIDSAIRALRHGAFEFIRKHGDPQELIDTVDRVLSRRRMEREHALMTIRLEHSERLH
                     RFLVEQSPDIIYTLDKNGVFIFVNGRVQALLGYSREELLGKHYSLIVHPDDQEHARFA
                     FHERRIGDRASTNVEIRLQTKSNGVRHFENRTIVTILSSQGIYATHEGPADLHFMGTS
                     GVARDITDRKKAEETIAFQAFHDLLTGLPNRTLFKDRLSVALKHAKRKRKRLGVMFID
                     IDRFKLVNDTYGHHEGDELLKNFALRARACLRSGDTLARQGGDEFTVLLPDLGSGDDA
                     ALIATKMLTELKRPFQVAGLDFFSTASIGIALYPDSGETPEVLLRNADIAMYQIKGQG
                     KNGYLQYTPEMHSGHSKRITLENDLRRALESGDQFEIHYQPQINFSQQRIIGMEALIR
                     WHHPRHGLLGPDAFIPLAEETGLIVALGEWVLENALRQMADWRARGFTSLQLAINLSP
                     KEFERQDLPERLLGRLRAHAIPAEVLDIEITEHLLMKDAEKNINMLKQ"
BASE COUNT          514 a          773 c          689 g          391 t
ORIGIN      
        1 gacatcggcg acctgctccg cgagttgctg acgctctatc gcgaaacgct gtttgactca
       61 aaaggcattg ccgtcgaaac ggtcctgccg gggcaggcga tccggatcgc ctgcgatcgc
      121 gacagcatca agcagatcct cctcaacctc tggaagaacg cctccgaagc gctgtcccgc
      181 gggcaacgca tcaggctttc gctgagcgac gatgtcgtgc accaggggcg gcgcttcgcc
      241 gaactgagga tcgaagacaa cgggccaggc atgggcgaag cggcgatcga cgcactgcat
      301 cgtccggccg ccgggcgaat cggcggcgca cgcggcatcg gcctgtcgat cgtcggcacg
      361 ctgacccagc gcctgggaat tccggtcacc tgccgcagca agccgggcct gggaaccatg
      421 atctcactgc tgttgccaac tccgaccgcg gccgagtcgc gggccagcga cagcaacact
      481 cgccatcaga cgatttcgtc gccggacacc agtcaggagt aacgatgaag gccatacccc
      541 aggtcgattt tttgccgcac gttcccgata gccacaagga gagcagctgc aacatcctga
      601 tcgtcgacga cgaacaacgc attcgggaga cctaccggca actgctatcc gcaccagagc
      661 gcagcatcga agactgcggc accggcagtg aagccatgcg ccgactggaa ggccgcgaca
      721 tcgacgtgct catcctcgat ctcaatctgc ccgacatcaa cgggctggaa atcatggaat
      781 ggatgctgcg ccggcatgtg ccgacggccg tcgtggtctt cagtggcgac gcatccatcg
      841 actcggcgat ccgcgcgttg cgccacggcg ccttcgagtt catccgcaag catggtgacc
      901 cccaggaact gatcgacacg gtggaccgcg tcctcagccg ccgccgcatg gagcgggaac
      961 acgccctgat gaccatccgg ctcgaacact ccgagcgact gcaccgcttt ctggtcgaac
     1021 agtcccccga catcatctac acgctcgaca agaacggcgt cttcattttc gtgaacggcc
     1081 gcgtgcaggc tctgctcggt tactcccggg aagaactcct cggcaagcac tattcactga
     1141 tcgtgcatcc ggacgatcag gaacacgccc gctttgcgtt ccacgaacgt cgcatcggcg
     1201 accgggcaag caccaacgtc gaaatccggc tgcagaccaa gtccaacggc gtccggcatt
     1261 tcgagaaccg taccatcgtc accatcctca gctcccaagg catctatgcc acccatgaag
     1321 gaccggccga cctgcacttc atgggtactt cgggcgtcgc gcgggatatc accgaccgca
     1381 agaaggccga ggaaaccatc gccttccagg cctttcacga tctgctcacc gggttgccca
     1441 accggacgct gttcaaggat cggctgagcg tggccctgaa gcatgcgaag agaaagagaa
     1501 aacggcttgg cgtgatgttc atcgacatcg accgcttcaa actggtcaat gacacctacg
     1561 gccatcatga aggcgacgag ttgctcaaga atttcgccct gcgcgcgcgc gcgtgcctgc
     1621 gctcgggaga cacgctggca cgtcagggcg gcgatgaatt caccgttctg ctgccggatc
     1681 tgggcagcgg cgacgatgcc gcgttgatcg ctaccaagat gctgaccgaa ctgaagcggc
     1741 cgtttcaggt cgccgggctg gatttcttca gcacggccag catcggcatc gccctgtacc
     1801 ctgacagcgg cgaaacgccc gaagtccttc tccgcaatgc cgacatcgcg atgtaccaga
     1861 tcaagggcca gggcaagaac ggctatctgc agtacacgcc ggagatgcac agcggacata
     1921 gcaagcggat cacgctcgag aacgacctgc gccgtgcgct ggaaagtggc gatcagttcg
     1981 agatccatta tcagccgcag atcaacttca gccagcaacg aatcatcggc atggaagcac
     2041 tgatccgctg gcaccacccg cgacatggcc tgctgggccc ggacgccttc atcccgctgg
     2101 ccgaggaaac cgggctgatc gtcgcacttg gcgaatgggt actcgaaaat gccctgcggc
     2161 agatggcgga ctggcgtgct cggggcttca ccagtctgca actggcgatc aacctctcac
     2221 cgaaggaatt cgaacggcaa gacctgccgg aaaggctcct ggggcgtctc agagcgcatg
     2281 ccatcccggc ggaggttctc gacatcgaga tcaccgagca cctgctgatg aaagacgccg
     2341 aaaagaacat caacatgctc aagcagc
//
LOCUS       DAOSIT010000372         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_38858_length_2367_cov_9.913495, whole genome shotgun sequence.
ACCESSION   DAOSIT010000372 DAOSIT010000000
VERSION     DAOSIT010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38858_length_2367_cov_9.913495"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLL14_08880"
     CDS             complement(<1..1115)
                     /locus_tag="PLL14_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG04082.1"
                     /translation="MNQEGEAALLDVRDLRIAFASGRSLLAAVDGISFEVAAGETLAL
                     LGESGCGKSATALGLLRLLPAAGRMLGGEVRFAGRDLLRLPEAEMRAVRGGGMAMIFQ
                     EPATSLNPVLTVGRQLAEVLERHLSLAGPAVRARSLELLTAVGLADPERRLGEYPFQL
                     SGGMKQRVMIAIALAGNPRLLIADEPTTALDVTIQAQILELLRRLQGERATGMLLITH
                     DLGVVAQMATRVGVMYAGQIVEEAPRAAFFSSPRHPYTQMLFAALPDLVRRGGRLETI
                     PGQVPPLSQMPRACRFAARCPHVWELCRQEAPPWRLVAVGHRIRCHLDGGLSASHAPG
                     ASDASPGAIVAAPATSVLTVENLCVHFPIRRGILQRTV"
     gene            complement(1112..>2367)
                     /locus_tag="PLL14_08885"
     CDS             complement(1112..>2367)
                     /locus_tag="PLL14_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04083.1"
                     /translation="IHYRPRLEAAAVPGVAPEGQPSVAYAVEVLSVLDGLLSSLRTRT
                     EKTYSAPLATRAYAKESIETRTADGQLRQVREYPRLKHAGAHLGADEARRDADVGMRL
                     LRALGLALLVWWALAAATAAGLARAEACSNLAAWRRIWRNESDFACNAVLAALGLLLL
                     LALPLLMLAADYHVLGTDKVGQDVLYQVLKSVRTALVIGLVTTLVMLPLSIFLGVLAG
                     YFRGWVDDLIQYLYTTLSSIPGVLLIAAAVLMMQVLIDTHPEWFATAAERADMRLLAL
                     CCILGVTSWTGLCRLLRGETLKLRELEYIQAAQAFGVSDLRIIARHILPNLMHIVIIA
                     LVMDFSSLVLAEAVLSYVGIGVDPTMISFGTMINNARMELAREPMVWWSLAAAFVFMF
                     ILVLAANLLADAVRDAFDPRLVGSA"
BASE COUNT          458 a          816 c          765 g          328 t
ORIGIN      
        1 acggtacgct gcaggatgcc gcggcggatc ggaaaatgca cgcacaggtt ttccaccgtg
       61 agtaccgacg ttgccggcgc cgccacgatg gccccaggcg aggcatcgga cgcacccggc
      121 gcatggcttg cactcagccc accatcaagg tgacagcgga tgcggtggcc gacggccacc
      181 agccgccacg gcggggcttc ctggcggcag agttcccaaa cgtggggaca gcgcgccgca
      241 aagcggcagg cgcgcggcat ttgtgacaat ggcggcacct gtccgggaat cgtctccagc
      301 ctgccgccac gccgcaccag atcgggcaga gcggcgaaca gcatctgcgt atagggatgg
      361 cgcggcgacg aaaagaaggc cgcgcgtggc gcctcctcga caatctgccc cgcatacatc
      421 acgcccaccc gggtggccat ctgcgcgacg acgcccaggt cgtgggtgat cagcagcatg
      481 cccgtcgcgc gctcgccctg caggcgccgc agaagttcca gaatctgcgc ctggatggtg
      541 acgtcgaggg cggtcgtcgg ttcgtcggcg atcaacagac gcggattgcc ggccagcgcg
      601 atggcgatca tcacccgctg tttcatgccg ccggaaagct ggaagggata ttctccgagc
      661 cgccgttccg gatcggccag tcccactgcc gtcagcagtt cgagcgaacg tgcgcgcacc
      721 gccggtccgg ccaggctgag gtgcctttca agcacttcag caagctgccg accaacggtg
      781 agcaccggat tgaggctggt cgccggctcc tgaaagatca tcgccatgcc gccaccgcgc
      841 acggcgcgca tctcggcttc gggcaagcgc aggagatcac ggccggcgaa acgcacttcg
      901 ccgccgagca tccgcccggc cgccggcaac aggcggagca ggcccagcgc cgtcgccgac
      961 ttgccgcaac cggactcgcc gagcagcgcc agcgtctcgc cggcggcgac ctcgaaactg
     1021 atcccgtcga ccgccgcgag cagcgaacgc ccgctggcaa aagcgatccg cagatcgcga
     1081 acgtcgagca gcgcggcttc tccctcctgg ttcatgccga ccccacaagg cgtggatcga
     1141 aagcgtcgcg tacggcatcg gccaacaggt tcgcggccag caccaggatg aacatgaaga
     1201 cgaaagcggc ggccagcgac caccacacca tcggttcgcg ggccagttcc atgcgtgcat
     1261 tgttgatcat cgtgccgaag ctgatcatcg tcggatcgac cccgatgccg acataggaca
     1321 gcaccgcctc ggccagcacc aggcttgaaa aatccatcac cagggcgatg atcacgatat
     1381 gcatcaggtt gggcaggatg tggcgggcga tgatccgcag gtcggaaaca ccgaaagcct
     1441 gcgccgcctg gatgtactcg agttcacgga gcttcagcgt ctcgccgcgc aacaagcgac
     1501 acagaccggt ccagctggtc accccgagga tgcagcacag cgccagcagg cgcatgtcgg
     1561 cacgctcggc ggcggtggcg aaccattccg gatgcgtgtc gatcagcacc tgcatcatca
     1621 gcacggcggc ggcaatcagc agcacgcccg ggatcgaact cagcgttgta tacagatact
     1681 gaatcaggtc atcgacccag ccccggaagt agccggccag cacccccagg aagatcgaca
     1741 gcggcaacat caccagcgtc gtcaccaggc cgatgaccag cgccgtgcgc acgctcttga
     1801 gcacctgata gagtacgtcc tggccgacct tgtcggtacc gagaacgtgg taatcggctg
     1861 cgagcatcag caacggcaag gcgagcagca acagcagacc gagcgcggcg agcaccgcat
     1921 tgcacgcaaa atcgctttcg ttgcgccaga tgcgccgcca cgccgcgaga ttcgaacagg
     1981 cttcggcgcg tgccagaccg gccgccgtcg ccgccgccag cgcccaccag accaggagcg
     2041 ccagtccgag cgcgcgcagc aggcgcatgc cgacatcggc atcccgccgc gcctcgtcgg
     2101 cacccagatg ggcgccggca tgcttcaggc gcgggtactc gcgcacttgt cggagttggc
     2161 cgtcggcagt acgcgtttcg atcgattcct tggcgtaggc gcgcgtcgcc agcggtgcgg
     2221 aataggtctt ctcggtacgc gtgcgcagtg agctcagcag gccgtcgagc accgacaaca
     2281 cctcgacggc gtaggccacc gacggctgcc cttcgggggc gacgcccggc actgccgcgg
     2341 cttcgaggcg cggccgatag tggatcg
//
LOCUS       DAOSIT010000373         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39224_length_2350_cov_7.174728, whole genome shotgun sequence.
ACCESSION   DAOSIT010000373 DAOSIT010000000
VERSION     DAOSIT010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39224_length_2350_cov_7.174728"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PLL14_08890"
     CDS             complement(<1..130)
                     /locus_tag="PLL14_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG04084.1"
                     /translation="MNRRLFIALLITLGTFCPLDAAAGPDCSRPFTLALHEHGLLYS"
     gene            405..809
                     /locus_tag="PLL14_08895"
     CDS             405..809
                     /locus_tag="PLL14_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04085.1"
                     /translation="MMEAVNALSSARVSTPAELTSPQNRVETPKPAGAPERQAEAPPS
                     VTVQISSAAREVAAREGASAAQDNNTTPTPPPPPRPPAAADAGSDTATDTSSSNAASA
                     QSAQVRQALQLFSDNAGSGVAQTKPSPLRASA"
     gene            896..1507
                     /locus_tag="PLL14_08900"
     CDS             896..1507
                     /locus_tag="PLL14_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HOG04086.1"
                     /translation="MIDQLSPTSAFTPSFQARPEAAQATGDAAAKAGGGVARAQQTGS
                     QLSEADQQKVRDLQQRDREVRAHEMAHVAAGGSLVRNGASFSYETGPDGQRYAIGGEV
                     SIDSSPGRTPEETRSKAERVKSAALAPADPSAQDRQVAALATRMAMQASMEQAIQAGG
                     AGGVAGNGTPASTTPELAPMLAAYAGSAAAAKAPSGSKVNVFA"
     gene            1730..>2350
                     /locus_tag="PLL14_08905"
     CDS             1730..>2350
                     /locus_tag="PLL14_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG04087.1"
                     /translation="MRSLQGKLVAFVVGLIATSVLLQGALFYQQLRGQLLESVAAETR
                     SAATGYAFAIADWIGAKSQMIRAAKTAIAAADAEHQFRLLAQGGGFDLVYAGYPDKRT
                     VFSEAQSLPPGYDPTTRPWYHDALAAGEASVIVTKPYEDAASKLLVISFASVMVKDGK
                     TVGVAAADIKIDRIVKEILGVRLGGDGFAFLLHKDGTVLAHPLQEAV"
BASE COUNT          436 a          799 c          760 g          355 t
ORIGIN      
        1 cggaatagag cagcccatgc tcgtgcagtg ccagcgtgaa tggccgcgag cagtctgggc
       61 cggcagccgc gtcgagcggg cagaaagtgc ccagggtgat cagcaaggca atgaaaaggc
      121 gacgattcat cgatatgacc cggcaacagg agtgccgcag gttcaggaca gggttcccgc
      181 ggctcggtga atagcatacg ctcgctacac cagcgtgcgc gatgaatagc gacagatgtc
      241 acggtcggga tgatgtcggc gcgcgcattc ggcttccgaa gcgtatagtc aaggttccgg
      301 cgggaaaata gaccccttgc agggaatccg gaccagcatc gctgatgacc agtcggactc
      361 ggagttttcc ggacacccct gcagtgtccg gccaggagat caccatgatg gaagccgtca
      421 acgccttgtc ctcagcgcgg gtcagtactc cggctgaact gacttccccc cagaaccgcg
      481 tcgaaacacc gaagccggcc ggcgcgcccg aacgccaggc cgaggcgccg cccagtgtca
      541 cggtgcagat cagttccgcg gcgcgcgagg tcgctgcccg cgagggggcc tcggcggcgc
      601 aggacaataa caccacgccg accccgcccc cgccgccgcg tcctcccgcc gccgccgatg
      661 ccggctctga taccgcgacg gacaccagca gcagcaacgc cgccagcgcc cagtcggcgc
      721 aggtccgcca ggcgctgcag ttgttcagcg acaacgccgg cagcggtgtc gctcagacca
      781 aaccctcgcc gctgcgcgct tcggcctgag cggccgcttt ttccgagcac cgcgacagcg
      841 gcgccagtct tgttctcgcc gaaggaggtt ctgacccgag cgcagtcatc ctgaaatgat
      901 tgaccaactg tcgccgacca gcgcattcac tccctcattc caggcccgcc ctgaagccgc
      961 gcaagccaca ggtgacgcgg cggcaaaagc cggcggcggt gttgcgcgcg cgcagcaaac
     1021 cggcagccag ttatccgagg ccgaccaaca gaaggtccgc gacctgcagc agcgcgaccg
     1081 tgaagtacgc gcccacgaaa tggcccacgt cgccgccggc ggcagcctgg ttcggaacgg
     1141 cgccagcttc agctatgaaa ccggcccgga cggtcagcgt tatgccatcg gtggtgaggt
     1201 ctcgatcgac agttctccgg gtcgcacccc ggaggagacg cgcagcaagg ctgaacgcgt
     1261 caagtccgcc gcgctggcgc cggcggaccc gtcggcgcag gaccggcagg tcgccgcgct
     1321 ggccacgcgc atggcgatgc aggccagcat ggaacaggcg atccaggccg gcggcgccgg
     1381 tggcgtagcc ggcaacggaa cacccgcatc caccaccccg gaactcgcgc ccatgctggc
     1441 cgcttatgcc ggcagcgcgg cggccgcgaa ggcgccgagc ggctcgaagg tcaacgtttt
     1501 cgcctgagcg ccgggcctcg cgcgatgttt ttcccgaccg gcggcgagcg aaaaacgaca
     1561 acaagccgag gctgacgagc aagatccggc aacacactgg agccacgcgg cttttgctct
     1621 gcctttccgc gtatgcgatg atgacgggcc cgcgcgactg ccgcctgggc gcggactgcc
     1681 ggcgctttgc ggcagcctcg ttcaccacca tgactgttgg agctcgcgga tgcgcagcct
     1741 gcaaggaaaa ctcgtggcct tcgtcgtcgg cctgatcgcc acgtcggtcc ttctccaggg
     1801 agcgctcttt taccagcaac tgcgcggcca gttgctcgag tccgtggctg ccgagacgcg
     1861 cagtgccgca accggctacg ccttcgccat cgccgactgg atcggcgcca aatcgcaaat
     1921 gatccgcgcc gcgaaaacgg caatcgcggc cgcggacgcc gagcatcagt tcagactgct
     1981 ggcccagggt ggcggtttcg acctggtcta tgccggctat cccgacaagc gaacggtttt
     2041 ctccgaagcg cagtcgctgc ctcccggtta tgacccgacg actcgcccct ggtatcacga
     2101 cgcgctcgcc gctggcgaag ccagcgtcat tgtcaccaag ccgtacgaag acgcggcctc
     2161 gaaactgctg gtgatctctt ttgcgtcggt gatggtcaag gacggaaaaa ccgtcggggt
     2221 ggctgcggca gacatcaaga tcgaccgcat cgtcaaggag atcctgggtg tccgtctggg
     2281 tggcgacggc tttgcgttcc tgctgcacaa ggatggaacc gttctggcac acccgcttca
     2341 ggaggcggtg
//
LOCUS       DAOSIT010000374         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39227_length_2350_cov_5.551198, whole genome shotgun sequence.
ACCESSION   DAOSIT010000374 DAOSIT010000000
VERSION     DAOSIT010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39227_length_2350_cov_5.551198"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLL14_08910"
     CDS             complement(<1..212)
                     /locus_tag="PLL14_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04088.1"
                     /translation="MPTALSHSSRSLLPAPWWLFPIHRAAAGSPGLGCMLRLMALFVL
                     LLVAAWPAVAAKRALVIGIDGYRNVTP"
     gene            complement(345..995)
                     /locus_tag="PLL14_08915"
     CDS             complement(345..995)
                     /locus_tag="PLL14_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04089.1"
                     /translation="MNRRRFLSMLPALPFAGAAQAEVGCHNRVEERFTLCQVGLAEAI
                     ADRMARLQPANSNWCWAACLEMIFACHGFHVGMQRIVAEAYGRQSNIGADTRMLAALL
                     SRRWIDDRGLPFASHCEVLWDAASGSRIRHTGGLAALRLAEGQPLLIGTRSHAMVLIA
                     LAFAHTGHPESLQVTHAVVLDPIPGRGRRPLTRDELSATSVLLSPRVTPLTVAPAR"
     gene            1183..2085
                     /locus_tag="PLL14_08920"
     CDS             1183..2085
                     /locus_tag="PLL14_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG04090.1"
                     /translation="MQARDSKRCRRDRKRGWPQWLALLAFAPLPAGAESPTDLPAVLL
                     AAGDIADCGPGAAATGKLLDSHPGLVLAVGDLAYPSGTGEDFQKCFAPTWGRFRARMK
                     AVPGNHDYMTAGAQPYFAALGAVAGPDRQGYYSLDYRGWHLIALNSHIPMRANSPQTV
                     WLKADLARTTARCLLAFLHLPRFSSGEGGDNDSTDVLWKTLASGGVSIVIAGHDHLYE
                     RFAPLSGDGSLDESDGVRGFTVGTGGAFLDTRPLWTRRHSEKVVTGRWGVLKLDLGND
                     GYRWAFLDINGQRWDSGQGKCRIR"
     gene            2129..2314
                     /locus_tag="PLL14_08925"
     CDS             2129..2314
                     /locus_tag="PLL14_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04091.1"
                     /translation="MLHPADIAALPNLDAGDDRHDDPITVKFAMMLLVFRGAQARKSP
                     HTAGSWACSLRDFQRSP"
BASE COUNT          425 a          684 c          812 g          429 t
ORIGIN      
        1 ggcgtgacgt tgcgataacc gtcaatgccg atcaccagcg cccgcttggc ggcaacagcc
       61 ggccaggccg caaccagcag cagcacgaaa agcgccatca gcctgagcat gcagcccaac
      121 cccggggaac cggctgctgc ccggtggatc gggaacagcc accagggcgc cggcaatagc
      181 gaacgcgacg agtgagacag ggcagtgggc atgggcgggt ttccttgagc tcacatgggc
      241 ggcggcccat ctttccggca tgggtcggaa gtataagatg cattacctac gtggaggcaa
      301 cccgaatggc acgggggcat tgccggtgcc aagccatcgc ctgactaacg ggcaggagcg
      361 accgtcaggg gggtgacccg ggggctgagc agcacactgg tcgccgacag ttcgtcgcgg
      421 gtcagtgggc gtcgtcctcg accggggatc gggtcgagca ccaccgcatg ggtgacctgc
      481 aggctttccg gatgaccggt gtgcgcgaag gcgagtgcga taaggaccat ggcgtggcta
      541 cgggtgccga tgagcagggg ttgaccctcg gcgaggcgca gtgcggcaag accgcctgtg
      601 tgcctgattc ggctacctga cgctgcgtcc cacaggactt cacaatggct ggcaaagggc
      661 agaccacggt cgtcgatcca tcggcgtgag agcagtgccg ccagcatgcg ggtatcggcg
      721 ccgatattcg attggcggcc gtaggcctcg gcgacgatgc gttgcatgcc gacatgaaat
      781 ccgtgacagg cgaagatcat ttccaggcag gcagcccaac accagttgct gttggcgggt
      841 tgaaggcgcg ccatgcgatc ggcgatggcc tctgccagac ccacctgaca cagggtgaac
      901 ctttcttcga cccggttgtg gcacccgact tcagcctgag cggctccggc gaacgggagt
      961 gccggcagca ttgacaggaa acgacgacgg ttcatgatcg gtatggggaa gttggcgata
     1021 atggaggaga tggaccgggg tgctgctggt caggaaggat ggtgtgttta tttgccctgg
     1081 tcgaatcgtt gctgcgtcac ggttgctcag gtagagatac gctgcgacgc ccgtttgata
     1141 tcgtccaggg attcgccgga caaacgatcg gttgaggaaa tgatgcaggc gagggattca
     1201 aagcgatgtc gtcgggaccg gaagaggggg tggccccagt ggcttgctct gcttgcgttt
     1261 gcgccgctgc cggcaggcgc ggaatccccg accgaccttc cggcggttct gctggcggcc
     1321 ggcgacattg ccgattgtgg gcccggtgcg gcggcaacag gcaaacttct cgactcgcac
     1381 ccgggtctgg tgcttgccgt tggcgatctg gcctatcctt ctggcactgg ggaagacttt
     1441 cagaaatgtt tcgccccgac ctggggacgt ttccgcgcgc gcatgaaggc ggtacccggc
     1501 aaccacgact acatgacggc cggagcacaa ccttactttg ccgcgctggg tgccgtcgct
     1561 ggtccggatc gccagggcta ctacagcctg gactaccgcg gctggcatct gatcgcactc
     1621 aacagccaca ttccgatgcg ggcgaattcg ccgcaaacgg tctggctgaa ggcggacctc
     1681 gcgcggacga cggcgcgctg tttgctggct ttcttacacc tgccccgttt cagctcgggc
     1741 gagggtggcg acaacgactc gacagatgtg ctttggaaaa ccctggcgtc cggcggcgtc
     1801 agtatcgtga tcgccgggca tgatcatttg tacgagcgct tcgcgccgtt gagtggcgat
     1861 ggctccctgg acgaaagcga cggcgtgcgt ggttttacgg tcggcaccgg cggtgccttc
     1921 ctggacacca ggccgttgtg gacacgtcgc cattccgaaa aggtggtgac cggccgttgg
     1981 ggtgtgttga agctggatct gggcaacgac ggctaccgct gggccttcct cgacatcaac
     2041 ggccagagat gggacagcgg acaaggcaag tgccggattc gctgaatggt tccgtgcttg
     2101 ctgcgtagcg gcggttgaat ccgggtggat gctgcacccc gcagatattg cagcgctgcc
     2161 gaatctcgac gctggcgatg accggcacga tgacccgatt acggtcaagt tcgccatgat
     2221 gctcctggtt ttccgcgggg cacaggcaag aaaaagcccg cacacggcgg gctcctgggc
     2281 gtgcagcttg cgcgactttc agcgctcgcc gtaagtccgg cggctggcag cggcgccatg
     2341 gctgcccttg
//
LOCUS       DAOSIT010000375         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39250_length_2349_cov_5.219704, whole genome shotgun sequence.
ACCESSION   DAOSIT010000375 DAOSIT010000000
VERSION     DAOSIT010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39250_length_2349_cov_5.219704"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..2170
                     /locus_tag="PLL14_08930"
     CDS             161..2170
                     /locus_tag="PLL14_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG04092.1"
                     /translation="MSMSSSEMSTRKQLLVMLSLPLLGLITYAIVTVWGAVSELRATH
                     LSKAAVEAAIATSQLVHEQQKERGLSGGFLASKGQQFGPELGRQRELTNQKLETLKAV
                     ARRLSETPVLAEALGQPLELALGKAGELSDFRRQVDLQAVKAAESFARYTAAISAGVD
                     VISAVAKATNNAEIARDATAYLMFVQAKEFAGRERATLNAAFAAKTFDPESFRRFVGI
                     VSAHDLYMRAFRTFASADASKLADATVKGNDVEEAVRMRKAAFEASPGEELDVPAGQW
                     FKASTARIDLMKAVESKLDEQIVGVLAKQEETALRTVVLALLASTLATIAALVLGSAM
                     IRRLMRRLGGEPATAAAIAHAIAGGDLTQSIEVTPGDTRSMMAAMRAMQESLREMISS
                     VHAAASEVTSQATRLAGASTEVSAASSASSQSAQTIATAVEQLSTSIQSISGNSDAVD
                     ATSKQTGEAAMNGKQIVNRTAKAMKSIAEVVDASSRSVNELGQQSEEIATIANVIREI
                     ADQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTRLSTLKISSTIERIRSCMT
                     EAERTMAAGTTGVRDALAEAERAELSMSNIQSSAAVVISSVDEITTLLREQSSSSKEI
                     SQNVSAIAASSAQVSGTVGSMAGIAQHLESLAGSLDRSVRRFQIA"
BASE COUNT          488 a          748 c          726 g          387 t
ORIGIN      
        1 aggtggaggg tagcctcagg ggccgcggtt acctcgaaat gaccggctat gccggcacac
       61 tgcggctctg agaaccgcga tgagccgcct ggacgcccca aagtggggca tgtccacgtt
      121 agaatcatgc ctttgcatgc gaacactggg ggtatgttta atgagcatga gctcctcgga
      181 aatgtcaacc cgcaagcagt tgttggtgat gctttcgctt cccctgctgg gcttgatcac
      241 ctacgcgatc gtcacggtct ggggcgcagt cagcgaactg cgcgcgaccc acctctcgaa
      301 agctgctgtc gaagccgcga tcgccaccag ccaattggtg catgagcagc agaaggagcg
      361 cgggctgtcc ggcgggtttc tggccagcaa gggtcagcag ttcggtcctg aactgggccg
      421 ccagcgggaa ctgaccaacc agaagctgga aaccctgaaa gccgtcgcca ggcgcctgag
      481 cgaaacaccc gtcctggcag aggcactggg ccagcccctt gagctggcgc ttggcaaagc
      541 cggcgaactg tccgatttcc gacgccaggt cgaccttcag gcggtcaagg cggcagaatc
      601 ctttgcccgc tataccgcgg ccatttcggc cggcgtagac gtgatcagcg ccgtcgccaa
      661 agccacgaac aatgccgaga ttgcgcgcga tgccaccgcc tacctgatgt tcgtccaggc
      721 caaggagttc gccggacgcg agcgggcaac cctgaacgcg gccttcgccg ccaagacatt
      781 cgatccggaa tcattccgcc gcttcgtcgg aatcgtcagc gcgcacgatc tttacatgcg
      841 agctttccgc acctttgcct ccgccgatgc cagcaaactc gccgacgcaa cggtcaaggg
      901 caacgacgtg gaggaagcgg tgcgcatgcg caaggctgcg ttcgaagcga gtccaggcga
      961 ggaactcgac gtccccgccg ggcaatggtt caaggcatcg acggcgcgta tcgacctgat
     1021 gaaagccgtc gaatcgaaac tggacgagca gatcgtcggc gtactcgcca aacaggaaga
     1081 gactgcactg cgtactgtgg tcctggcgtt gctggcctcg accttggcca ctatcgccgc
     1141 actggtattg ggcagcgcga tgatccggcg cctgatgcgc cgcctcggcg gcgaaccggc
     1201 gaccgccgca gccatcgccc acgccattgc cggtggtgac ctgacacagt cgatcgaggt
     1261 gactccgggc gacacacgga gcatgatggc cgccatgcga gccatgcagg aaagcctgcg
     1321 tgaaatgatt tcctcggtgc atgccgcagc gagcgaagtc acatcgcaag ccacccggct
     1381 ggccggcgcg tcgaccgagg tatcggcggc ctcgtcggca agcagccagt cggcacagac
     1441 cattgccacc gcggtggaac agttgtcgac ttcgatccag agcatttcag gcaactcgga
     1501 tgcggtcgat gccacgtcaa aacagaccgg cgaagccgcc atgaacggca aacaaatcgt
     1561 gaaccgcacg gccaaggcga tgaaatcgat cgccgaggtc gtcgatgcct cgtcgagatc
     1621 ggtcaacgaa cttggccagc aatcggagga gattgccacc atcgccaatg tgattcgcga
     1681 gattgccgac cagaccaacc tgctggccct caacgccgcg atcgaggccg cccgcgccgg
     1741 tgagcaggga cgcggcttcg ccgtcgttgc cgacgaggtg cgcaagcttg ccgagcggac
     1801 gcgcctatcg acgctgaaaa tctcctcgac cattgaaaga atccgctctt gcatgaccga
     1861 agccgaaagg accatggcgg ccggtactac aggcgttcgc gacgccctcg ccgaagcgga
     1921 gcgcgccgaa ctgtcgatga gcaatatcca gtcgtcagcc gcagtggtca tttccagtgt
     1981 cgacgaaatc accacactcc tgcgcgaaca aagcagcagc agcaaggaaa tatcgcagaa
     2041 tgtcagtgcc atcgccgcaa gcagcgcgca ggtcagcggc actgtcggtt cgatggccgg
     2101 gattgcccag cacctcgaat cactcgccgg ttccctcgat cggtcggtca ggcgcttcca
     2161 gatcgcttga gcgcattggt gggagtgcgc gccggtgcgc tcacagcccc aggaaatcct
     2221 tgatcatcgc cagaatgccg gccttcggat gcgtagccgg gccgtcggcc gggacgccgc
     2281 tgaactggcg cacgaaattg tccatttcga cctcgcgacg ggcaagaatc tcggcaatcg
     2341 cctcggcga
//
LOCUS       DAOSIT010000376         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39429_length_2341_cov_10.085739, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000376 DAOSIT010000000
VERSION     DAOSIT010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39429_length_2341_cov_10.085739"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..85
                     /locus_tag="PLL14_08935"
     CDS             8..85
                     /locus_tag="PLL14_08935"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HOG04093.1"
                     /translation="MIHDIQVEATFPDGTKLVTVHHPIV"
     gene            101..673
                     /locus_tag="PLL14_08940"
     CDS             101..673
                     /locus_tag="PLL14_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HOG04094.1"
                     /translation="MNKRTVMFLVTGLLCLPVVAIAHPGHAGDGFGAGFMHPFLGLDH
                     LLAMVVVGIWSVLHSRRVWLAPLCFVCFLAIGAMLGHGGLVVPQLEPLVAASVLVLGL
                     MLTLPLGRGTSVPLAVIGAFALCHGMAHGGELGAAGSVLAGIVIGSAILHGAGMLLAS
                     HVLQQREAWAHRLGQAVALVGGYLLVNTLF"
     gene            697..1005
                     /locus_tag="PLL14_08945"
     CDS             697..1005
                     /locus_tag="PLL14_08945"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HOG04095.1"
                     /translation="MIPGEMAVATGEIELNAGRETIRLSVANSGDRPIQVGSHYHFAE
                     TNVALVFDRAAAHGFRLDIAAGTAVRFEPGQTRSVQLVALSGERRVYGFNALVMGALQ
                     "
     gene            1002..>2341
                     /gene="ureC"
                     /locus_tag="PLL14_08950"
     CDS             1002..>2341
                     /gene="ureC"
                     /locus_tag="PLL14_08950"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOG04096.1"
                     /translation="MSVKISRQAYAEMFGPTIGDRVRLADTELWVEVEDDFTIYGEEV
                     KFGGGKVIRDGMGQGQRPAAEVADTVITNALIVDHWGIVKADIAIKGGCIAGIGKAGN
                     PDIQPGVTIAIGGGTEIIAGEGLLVTAGGIDSHIHFICPQQIEEALMSGVTTMLGGGT
                     GPATGTYATTCTPGPWHLHRMLEAADAFPMNLGFFGKGNVSLPEPLREQVRAGAIGLK
                     LHEDWGTTPAAIDNCLAVAEEMDVQVAIHTDTLNESGFVETTLAAFKGRTIHTFHTEG
                     AGGGHAPDILRAVGEANVLPSSTNPTRPYTVNTVDEHLDMLMVCHHLDSAIAEDVAFA
                     ESRIRRETIAAEDILHDLGALSMMSSDSQAMGRVGEVIIRTWQTAHKMKVQRGHLPAA
                     GTGGAPPTANDNFRVKRYLAKYTINPAITHGISHLVGSIEVGKLADLVLWKPAF"
BASE COUNT          418 a          752 c          747 g          424 t
ORIGIN      
        1 gccggaaatg atccacgaca tccaggtcga agccaccttc ccggacggta ccaaactggt
       61 gaccgtccat caccccatcg tttgaaccag ggagtcgaag atgaacaagc gtacagtcat
      121 gtttctcgtc accggtctgc tgtgcctgcc ggttgtcgcc atcgcccacc ctgggcacgc
      181 cggagacggc ttcggcgctg gcttcatgca ccctttcctt ggcctcgacc atctgctggc
      241 gatggtcgtg gtgggtatct ggagcgttct ccatagccgc cgcgtgtggc tcgcgccact
      301 ttgtttcgtc tgcttcctgg cgatcggcgc gatgcttggc cacggcggcc tggtcgtccc
      361 acaactcgaa ccgctcgtcg cagcctcggt ccttgtcctt ggcttgatgc tgaccttgcc
      421 gcttggccgt ggcacatcgg tgccactggc ggtcatcggc gctttcgcgc tctgccacgg
      481 catggcgcac ggcggcgaac tgggggctgc gggcagcgtg ctggccggaa tcgttatcgg
      541 ttcggccatt ctgcacggcg cgggaatgtt gcttgctagc catgtactgc agcaacgcga
      601 ggcctgggcg catcgtcttg gtcaggcggt ggcgctggtg ggcggttacc tgctggtcaa
      661 caccctgttc tgatggacga aaatgaaggg gcgaaaatga ttcccggtga aatggcagtc
      721 gccacgggcg aaatcgaact caacgccggc cgcgagacga tccggttgag cgtggccaac
      781 agcggcgatc ggccgatcca ggtcggttcg cattatcact ttgccgaaac caacgtcgcg
      841 ctggttttcg accgcgccgc ggcgcatggt tttcggctcg atattgccgc cggtaccgcc
      901 gtgcgcttcg agccgggaca gacgcgcagc gtgcaactgg tggcgctgtc cggcgagcgc
      961 cgggtttacg gtttcaacgc cctggtgatg ggagcactgc aatgagcgtc aagatctccc
     1021 ggcaggccta cgccgagatg ttcgggccga cgatcggtga tcgggtacgt ctggccgaca
     1081 cagaactgtg ggtcgaagtc gaagacgatt tcacgatcta cggtgaggaa gtcaagttcg
     1141 gcggtggcaa ggtcatccgt gacggcatgg ggcagggtca gaggccagcc gctgaagtcg
     1201 ccgacacggt gatcaccaat gcgctgattg ttgaccactg gggcatcgtc aaggccgata
     1261 tcgccatcaa gggcgggtgc atcgccggca tcggcaaggc cggcaacccc gacatccagc
     1321 cgggggtgac tatcgccatt ggcggcggca ccgagatcat cgccggcgaa ggtctgctcg
     1381 tcactgccgg cggtatcgac agccacatcc atttcatctg tccgcaacag atcgaggaag
     1441 cgctgatgtc gggggtgacc acgatgctcg gcggcggcac ggggccggcc accggcacct
     1501 acgccaccac ctgtacgccg ggaccctggc acctccaccg catgctcgaa gcggccgacg
     1561 cgtttccgat gaacctcggt ttcttcggca agggcaacgt ttccttgccc gaacccttgc
     1621 gcgaacaggt gcgcgccggc gccatcgggc tcaagctgca cgaggactgg ggaacgacgc
     1681 cggcggcgat cgacaactgc ctggcggtag ccgaagagat ggacgtgcag gtggccatcc
     1741 acaccgatac gctcaacgaa tcgggcttcg tcgaaacgac gctcgccgct ttcaaggggc
     1801 ggacgatcca cacctttcat accgaaggcg ccggtggcgg gcacgcgccg gacatcctgc
     1861 gtgccgtcgg cgaggccaac gtcctgccct cgtcgaccaa cccgacccgg ccctacacgg
     1921 tgaacaccgt cgatgaacac ctcgacatgc tgatggtttg ccatcacctg gactcggcga
     1981 ttgccgaaga cgtggctttc gccgaaagcc gcatccgccg cgagacgatc gccgccgaag
     2041 acatcctgca cgacctcggc gcgctgtcga tgatgtcctc ggactcgcag gcgatgggcc
     2101 gtgtcggcga agtgatcatc cgcacctggc agaccgcgca caagatgaag gtgcagcgtg
     2161 gccacctgcc ggcagcggga actggaggag cgccgccgac cgccaacgac aatttccgcg
     2221 tcaagcgcta cctcgccaaa tacaccatca atccggcgat cacgcatggc atcagccacc
     2281 tcgtcggttc gatcgaggtc ggcaagctcg ccgacctggt actctggaag ccggcgttct
     2341 t
//
LOCUS       DAOSIT010000377         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39619_length_2333_cov_11.035119, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000377 DAOSIT010000000
VERSION     DAOSIT010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39619_length_2333_cov_11.035119"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLL14_08955"
     CDS             complement(<1..250)
                     /locus_tag="PLL14_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04097.1"
                     /translation="MADINFMGRLSNLWSGFVSLWITDVEKRHPEIAYQNAIDSMIEK
                     YGKLKSATAAIMRRREEISARLESERGELAAISADLNAA"
     gene            complement(348..1232)
                     /locus_tag="PLL14_08960"
     CDS             complement(348..1232)
                     /locus_tag="PLL14_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04098.1"
                     /translation="MEAAKPSYVREVLTSQTNLYAGLGSLALGVVLSIPFGFGIGALP
                     LIAFAAGDILAALHIPSLPTFRDKVDRRWRARSRQASREQLMLEIQKRAGKRALPAAT
                     LRTYQRMYERVQSLYQRAETGHGRLAYRDVEQLDDVSLEYLALWLALLVMDDRAESVN
                     LREVETKVAALERELAAPRPGTDLRQLQKARADYLAVIERHNRMLSRRRALEAAMLSM
                     PDQLDEIYQTIMTLPVSEEVGTRLEEAIGKLRLQEDIEADLASDLAEAVPGVAMPTAA
                     AAGRQRRLVAVGSSGRAG"
     gene            complement(1235..2107)
                     /locus_tag="PLL14_08965"
     CDS             complement(1235..2107)
                     /locus_tag="PLL14_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG04099.1"
                     /translation="MTLEAAKPVAAASAAAAKPVAVVIKDIVQEYPAPGGGVTRVIDK
                     VSLSFDQPGINMLLGPSGCGKSTILHMLGGVRPMGVDTPTAGSVLIDGVECHAPHDDA
                     VMVFQRYANRPDLSVFDNVAFPFRLKLWRSQVPEADWRQRVADILKAVGLSDKVGLRP
                     AQLSGGQNQRVALARALVLRPRILLMDEPFGALDAQTREEMQQLLIGLYQAWPCLVVF
                     VTHDVTEALMLGDRVIVLSTQPARVADDFLIDEARPRSAAWQRSREAQALEERILNQL
                     HQASPGRGQVRVTV"
     gene            complement(2104..>2333)
                     /locus_tag="PLL14_08970"
     CDS             complement(2104..>2333)
                     /locus_tag="PLL14_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04100.1"
                     /translation="ADAVIVAFSVMWTYITVAEYVNAREGLGQLIQNARRFSAWDQVF
                     AGIMVIIALALATYQLMVWLKRRLYPWETQL"
BASE COUNT          396 a          800 c          746 g          391 t
ORIGIN      
        1 gcgccgcatt gaggtcggcg gagatcgccg ccagttcgcc acgttcgctt tccaggcgcg
       61 ccgagatttc ctcgcgccga cgcatgatcg ccgccgtggc gctcttcaac ttgccatact
      121 tctcgatcat cgagtcgatg gcattctggt aagcgatctc gggatgccgc ttttcgacat
      181 cggtgatcca cagcgagaca aagcccgacc agaggttgga caggcgcccc ataaaattga
      241 tgtcagccat tgccttcttc ctttcgtgag ccggtcaacc ggccgttcct gagttaccca
      301 atcatgctgc gcatcggtca atcgtcgacc accttcgtcg cggtcggcta gccggcacgg
      361 ccggaactgc ccaccgccac caggcgccgc tgccgcccgg ccgccgccgc ggtcggcatc
      421 gccacgcccg gcacggcttc ggcgagatcg ctggccaggt cggcctcgat gtcttcctgc
      481 agccgcagct tgccgatcgc ctcttcgagt cgcgtcccga cctcctcgct caccggcagg
      541 gtcatgatcg tctgatagat ctcgtccagt tggtccggca tcgacagcat cgccgcttcg
      601 agcgcccgcc gccgactcag catgcggttg tgccgctcga tgacggccag gtaatcggcg
      661 cgggccttct gcaactgacg caggtcggtg ccaggacgtg gcgccgccaa ctcccgttcc
      721 agagccgcga ccttggtctc gacctcgcgc agattgaccg actcggcacg gtcgtccatg
      781 accagcagtg ccagccacag cgccagatat tcgagggata cgtcgtccag ttgctcgacg
      841 tcccggtaag cgagccggcc atgcccggtt tctgcccgct ggtacagcga ctgcacgcgc
      901 tcgtacatgc gctgataagt gcgcagcgtc gccgcgggaa gcgcgcgctt gccggccctt
      961 ttctgaatct ccagcatcaa ctgctcgcgc gaggcctggc gggagcgcgc ccgccagcga
     1021 cggtcgacct tgtcgcggaa ggtcggcagc gacgggatgt gcaaggccgc aaggatgtcg
     1081 ccggcggcaa aggcgatcag cggcagggcg ccgatgccga agccaaacgg aatcgacagc
     1141 acgacgccaa gggccagcga cccgagaccc gcgtacaggt tggtctgact ggtcagaacc
     1201 tccctgacgt aggagggctt ggccgcttcc atgcctagac cgtaacccgg acctggcccc
     1261 tgccgggact ggcctggtgc agttgattga ggatgcgttc ctcgagcgcc tgcgcctcgc
     1321 gcgagcgctg ccaggcggcc gaccgcggac gcgcttcgtc gatcaggaaa tcgtcggcga
     1381 cgcgcgccgg ctgtgtggac aggacgatga cgcggtcacc gagcatcagc gcttcggtga
     1441 cgtcgtgcgt cacgaagacg accaggcacg gccaggcctg gtacaggccg atcagcaact
     1501 gctgcatctc ctcgcgggtc tgcgcgtcga gtgccccgaa gggctcgtcc atcaacagaa
     1561 tgcgcggccg caggacgagc gcccgcgcca gcgccacgcg ctggttctgt ccacccgaga
     1621 gctgcgccgg ccgcaggccg accttgtccg agagcccgac cgccttgagg atgtcggcga
     1681 cgcgttgccg ccaatccgct tccggcacct gcgaacgcca gagcttgagg cgaaacggaa
     1741 acgcgacgtt gtcgaacacc gacaggtccg gtcggttggc atagcgttga aaaaccatca
     1801 ccgcgtcgtc gtgcggcgcg tggcattcga ccccgtcgat cagcacgctg cccgcagtcg
     1861 gggtgtcgac gcccatcggc cgcacgccgc cgagcatgtg caggatcgtg ctcttgccgc
     1921 agcccgacgg gccgagcaac atgttgatgc cgggctggtc gaagctcagc gaaaccttgt
     1981 cgatgacgcg cgtgacgccc ccgccgggcg ccgggtactc ctggacgatg tccttgatga
     2041 ccaccgcgac gggctttgcc gccgctgcgg aagccgccgc cacgggtttc gcggcctcca
     2101 gggtcatagc tgcgtctccc agggatacag gcggcgcttg agccagacca tcagttgata
     2161 ggtggccagg gccagggcga tgatcaccat gatgccggca aagacctgat cccaggcgct
     2221 gaaacgccgc gcattctgga tcaactgccc gagtccttcg cgcgcattga cgtattcggc
     2281 gacggtgatg taggtccaca tcaccgagaa agcgacgatg acggcatcgg cga
//
LOCUS       DAOSIT010000378         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39877_length_2321_cov_9.172992, whole genome shotgun sequence.
ACCESSION   DAOSIT010000378 DAOSIT010000000
VERSION     DAOSIT010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39877_length_2321_cov_9.172992"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLL14_08975"
     CDS             complement(<1..617)
                     /locus_tag="PLL14_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04101.1"
                     /translation="MAKKTNHTASNLRLRTSVLAVASALTLTALPVATEAAGLGKVNV
                     FSALGQPLRAELEVFATREELAGMKAQLASQDAFKQAGVDFAPTLSGITLAVDKRPNG
                     QAIIRLTSTKPINDPFVDLLLELNWPTGRLIREYTFLLDPPEFTGKATAAAAPAIARP
                     VAVARQTVDTRPLEDKPARPAPYRGRASESVQKPMDGGSTYEVKRG"
     gene            complement(755..1885)
                     /gene="asd"
                     /locus_tag="PLL14_08980"
     CDS             complement(755..1885)
                     /gene="asd"
                     /locus_tag="PLL14_08980"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG04102.1"
                     /translation="MMKLGLVGWRGMVGSVLMQRMVEEGDFEHVEPLYFSTSAVGGKA
                     PLLAGKEAAPLQDAMSIEALSRMDIVITCQGGDYTKEVFPRLRGAGWRGHWIDAASTL
                     RMHDDAVIILDPVNLEVIRDALARGGRNWIGGNCTVSLMLMGLGGLFRHDLVEWISAM
                     TYQAASGAGAQNMRELLQQMGALHDAVKAELAEPASAILEIDRKVAESMRSAAFPTTN
                     FRNTALAGSVIPWIDAEVEGGQSKEEWKGGAECNKILGLPAFRTPGSIPVDGLCVRVG
                     AMRCHSQGLTIKLRRDVPLADLEDIIAQANPWVKVVPNQREASERELTPAAVTGTLSV
                     PVGRLHKLAMGSEYLGAFTVGDQLLWGAAEPLRRMLRILIDN"
     gene            complement(1912..>2321)
                     /locus_tag="PLL14_08985"
     CDS             complement(1912..>2321)
                     /locus_tag="PLL14_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG04103.1"
                     /translation="VDNAAMQLVRNPKQFDVLVTGNLFGDILSDEAAMLTGSIGMLPS
                     ASLDADNKGLYEPCHGSAPDIAGHGLANPLATILSVAMMLRYTFAHEEAALRIESAVR
                     KVLAQGYRTADIYEPGTQKLGTRAMGDAVLAVL"
BASE COUNT          434 a          774 c          683 g          430 t
ORIGIN      
        1 ccgcgcttga cctcgtaggt actgcccccg tccatgggtt tctgcaccga ttcactggcc
       61 cggccacggt acggcgcggg cctggccggc ttgtcctcca gcgggcgcgt gtcgacggtc
      121 tgtcgcgcta cagccactgg cctggcgatg gcaggcgctg cggccgctgt cgccttgccg
      181 gtgaactcgg ggggatcgag caggaaggtg tattcacgaa tcaagcggcc ggtcggccag
      241 ttgagttcaa gcagtagatc gacgaacgga tcgttgatcg gcttggtcga ggtcaacctg
      301 atgatcgcct gaccattcgg gcgcttatcg acggccagcg tgatccccga caaggtcggc
      361 gcgaagtcca cacccgcttg tttgaaggcg tcctgcgaag caagctgggc tttcatcccg
      421 gcaagttctt cgcgggtcgc aaagacttcc agttccgcgc gcaagggttg tcccagcgcc
      481 gaaaagacgt tcaccttgcc gagcccggca gcctcggtag ccacaggcaa agccgtgagc
      541 gtcagtgccg acgccaccgc caataccgag gtccgcagtc tgagattgct tgctgtgtga
      601 tttgttttct tggccacggc gccaccttga gcgtcgaaat cgggattatt aaaataacat
      661 catgaagtta gccatgcaag ctaaacatgc ggcggtagcg gcagccggct gcaccccgga
      721 aaccggtcgc acaagcccgg tttttctcca tcaatcagtt atcgatcaga atccgaagca
      781 tccggcgtag cggttcggcc gccccccaca ggagttggtc accgacagtg aacgcaccaa
      841 ggtactccga ccccatcgcc agcttgtgca aacggcccac cgggaccgag agagtgccgg
      901 tgacggccgc cggcgtcagt tcgcgctcgc tggcctcgcg ttgattcggc accaccttca
      961 cccaggggtt ggcctgcgcg atgatgtcct caagatcggc cagaggaacg tcccgcctga
     1021 gcttgatggt caggccctgc gaatggcagc gcatcgcgcc gacacgcacg cacaggccat
     1081 cgaccggaat gctcccgggg gtgcgaaacg cgggcagccc gagaatcttg ttgcactcgg
     1141 cgccgccctt ccactcctcc ttggactgac cgccctcgac ctcggcatcg atccagggaa
     1201 tgacgcttcc ggccagcgcc gtgttgcgga aattggtggt cggaaaggcc gctgaacgca
     1261 tgctttcggc aaccttgcga tcgatctcga ggatcgccga agccggttcg gccagttcag
     1321 ccttaaccgc gtcgtgcaac gcgcccatct gctgcagcag ttcacgcatg ttctgcgccc
     1381 cggctcccga agcggcctga taggtcatgg cgctgatcca ttcgaccagg tcgtgccgga
     1441 acaggccgcc aagccccatc agcatcagcg aaaccgtgca gttgccgccg atccagttgc
     1501 gaccgcctct ggccagagca tccctgatga cctcaaggtt caccgggtca aggatgatca
     1561 cggcgtcgtc gtgcatgcgc agggtcgaag ctgcgtcgat ccagtgtcct cgccagccgg
     1621 cgccgcgcag gcgggggaag acctctttgg tatagtcgcc gccctgacag gtgatgacga
     1681 tatccatccg gctcagggcc tcgatcgaca tcgcgtcctg tagcggcgcg gcctccttgc
     1741 cagccagcag cggcgccttg ccgccgactg ccgaggtcga aaaatagagc ggctcgacgt
     1801 gctcgaaatc cccctcttcg accatccgct gcatcagcac cgagcccacc atgccccgcc
     1861 aacctaccag accaagcttc atcatgttct tacctttttc cctaacccga ttcaaagtac
     1921 cgccagcact gcgtcaccca tggcccgcgt accgagcttc tgcgtaccag gctcgtagat
     1981 gtcggccgtc cgatagccct gcgccagcac tttcctgacc gcgctctcga tgcgcaaagc
     2041 ggcttcctca tgcgcaaacg tgtagcgcaa catcatcgcc acggaaagga tcgtggccag
     2101 cggattggcc agtccatgac cggcgatgtc cggagcggaa ccgtgacaag gctcgtacaa
     2161 ccccttgttg tcggcatcca gcgaggccga cggcagcatg ccgatcgaac cggtgagcat
     2221 cgccgcttcg tcagacagga tgtctccgaa caggttgccg gtgaccagca cgtcgaactg
     2281 cttcggattg cgcaccagtt gcatcgccgc attgtcgacc a
//
LOCUS       DAOSIT010000379         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39948_length_2318_cov_8.121078, whole genome shotgun sequence.
ACCESSION   DAOSIT010000379 DAOSIT010000000
VERSION     DAOSIT010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39948_length_2318_cov_8.121078"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLL14_08990"
     CDS             complement(<1..1390)
                     /locus_tag="PLL14_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOG04104.1"
                     /translation="MADAFWSAAAPAAVAGAGFSLLIERLLQPPTRPLWRRPAAALAI
                     HLGLWLLLFSCLLLVLQRPFFAAGALLAGMLFLVLVSNAKFHALNEPFVFQDFEYFSD
                     ALRHPRLYLPFLGAGRALLALLALAAAVTGGLAIERSLLSGLPVTDFLAGAGGLALAG
                     LLLLWLGLAKQLSVSFDAAADLRRLGLLAALWRYGEEELLPFKVSLPYEHLGAGPPVR
                     AHPAHLVVVQSESFFDVRRLFSGIRTDVLREFDALQAAAMWQGRLQVSAWGANTVRTE
                     FAFLSGLAIDSLAVHRFNPYRRLARQDLATLASYLRRCGYRTVCVHPYAASFYARDKV
                     YPRLGFDEFIDIRSFSGAPKSGPYVSDLAVAEKVCSLLASSSEQPLFVFVITMENHGP
                     LHLEDPLPGDSERCYTAPPAAGCDDLTIYLRHLRNADNMAGRLRQQLAALPGDGCLCW
                     FGDHVPILPEVYR"
     gene            complement(1383..2159)
                     /locus_tag="PLL14_08995"
     CDS             complement(1383..2159)
                     /locus_tag="PLL14_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG04105.1"
                     /translation="MNPPAFPASVLITGASGGIGGALAVVYAEAGRTLVLQGRNAPRL
                     AELAALCEARGARVLSQVLDLRDRAALADWLRVLCAQEQLDLVIVNAGLNTHVGPDGA
                     AEGWPEVQDLLEVNVMAAWATVNAVLPAMRLRGQGQIALISSLAAFYGLPVAPSYCAS
                     KAAIKVYGEAMRGWLAAEGIRINVVMPGYVESSMCRAMPGPKPFLWSAERAARYIRRG
                     LERNRARISFPFPLNLGTWFLSVLPPTVSERILRLLNYGG"
BASE COUNT          485 a          793 c          735 g          305 t
ORIGIN      
        1 cccgatacac ctccggcagg atcggcacat gatccccgaa ccagcacaag caaccgtccc
       61 ccggcagagc cgccagttgc tgccgcagcc tgccggccat attgtcggcg ttgcgcagat
      121 gacggagata gatcgtcagg tcatcacagc cggcggccgg cggcgccgtg tagcagcgtt
      181 cgctatcgcc gggcagcgga tcctccagat gcaagggtcc gtggttctcc atggtgatca
      241 caaaaacaaa aagcggttgc tccgaggaac tcgccagcaa ggaacagact ttctcggcaa
      301 ccgccaggtc gctgacataa ggcccgctct ttggcgcgcc ggaaaagctg cgaatgtcga
      361 tgaactcgtc gaagccgagt cgcgggtaca ccttgtcgcg cgcataaaag ctggcagcgt
      421 agggatgcac gcagacggtg cggtagccgc atcgccgcag gtaactggca agggtggcca
      481 gatcctggcg cgccaatcgg cgataggggt tgaagcgatg cacggccagc gagtcgatcg
      541 caagtcccga cagaaacgca aactcggtgc gcaccgtatt ggccccccag gccgacacct
      601 gcaaccgtcc ctgccacatc gcggccgcct gcaaggcgtc gaactcgcgc aggacatccg
      661 tgcggattcc ggaaaacagg cgccgcacat cgaagaagga ctcgctctgc accaccacca
      721 gatgcgccgg atgcgcgcga accggtggcc ccgcaccgag gtgctcatag ggcaaggaaa
      781 ccttgaaggg cagcagttct tcctcgccat aacgccacag ggcagccagc aagcccagtc
      841 gccggaggtc agccgcggcg tcgaagctga cggacagctg ctttgcgagg cccagccaca
      901 gcagcagcag cccggccagc gccaacccgc ccgcgccggc caggaagtcg gtgaccggca
      961 gcccggacag cagcgatcgc tcgatcgcca ggccgccggt gaccgcggca gccaaggcca
     1021 gcagcgccag cagcgccctg ccggcgccca ggaaaggcag atacaggcgg ggatgccgca
     1081 aggcgtcgct gaaatactcg aaatcctgga acacgaacgg ttcgttcagg gcatggaact
     1141 tggcgttgct caccagaacc aggaacagca ttcccgccaa cagcgcacca gcggcaaaga
     1201 aagggcgctg caggaccagc agcagacagg aaaacagcag cagccagagt ccgaggtgga
     1261 ttgccagggc cgcagccggt cgcctccaca aaggtcgcgt cggcggctgc aggagacgct
     1321 cgatcaacag tgaaaacccc gcgccagcca cggccgccgg tgccgccgcc gaccagaacg
     1381 cgtcagccac cgtaattgag caggcgcagg atgcgctcgg aaaccgtcgg aggcaggacc
     1441 gacaggaacc aggttcccag attgagcgga aaaggaaagc tgatgcgtgc ccggttacgc
     1501 tccagaccgc gccggatgta gcgggcggcg cgctcggcgg accacaggaa tggcttcggt
     1561 ccgggcatcg cgcggcacat cgatgattcc acgtaacccg gcatgacgac gttgatgcgg
     1621 atgccctcgg ctgccaacca gccgcgcatc gcctcgccat agaccttgat cgcggccttg
     1681 ctggcgcagt agctcggcgc caccggcaac ccgtaaaacg cggccaggga actgatcagg
     1741 gcgatctgtc cctgtccacg caggcgcatc gccggcagca ccgcatttac cgtcgcccac
     1801 gccgccatga cgttcacctc cagcaggtcc tgcacctccg gccagccctc ggcggcaccg
     1861 tccggcccga catgcgtgtt caggccggca ttgacgatca ccagatccag ttgctcctgc
     1921 gcacagagca cacgtagcca gtccgccagc gccgcgcggt cgcgcagatc gaggacctgc
     1981 gacaacacgc gagcgccgcg cgcctcgcag agcgccgcca actccgccag gcgaggcgca
     2041 ttgcgtccct gcaggacgag ggtcctgccc gcttcggcat agaccacggc cagagcgccg
     2101 ccaataccgc cgctggcgcc ggtaatcagc acgctcgcgg ggaaagccgg tgggttcaca
     2161 ggagttcgtc cagcggcgag cgctcgggct ccagcagccg ccggcagttg ttgaccgcca
     2221 tgcggatgcc ggcttgggaa tagaaccccc cattgacctg cgtggtatgg atcaccgtat
     2281 ttcgaaagcg ccggaacagt tcggcatccg gtttgccg
//
LOCUS       DAOSIT010000380         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_39992_length_2316_cov_8.112782, whole genome shotgun sequence.
ACCESSION   DAOSIT010000380 DAOSIT010000000
VERSION     DAOSIT010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39992_length_2316_cov_8.112782"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PLL14_09000"
     CDS             complement(<1..1772)
                     /locus_tag="PLL14_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOG04106.1"
                     /translation="MSEAAVNIVHEHRLTLAEVLGWLTDDGLVNAPDADQLLKESRLQ
                     RLVAHPLVVIADQQWKSAQEPHRPLNLEGLGEWLAGRVGLDYHHIDPLKVDFTAVTDV
                     MSSAYATRFGILPIQVTTREIVVATTQPYLRDWEKEIRSIAKKEIRRVMANPVDVARY
                     LVEFYNLARSVKKAAQLGGPSGNLSSFEQLVELGRTNRQFDANDQHIVNIVDWLWQYA
                     FEQRASDIHIEPRREIGIVRFRIDGVLHQVYQIPMSVMAAMVNRVKILGRMDVVEKRR
                     PQDGRIKTRTADGQEAELRLSTLPTAFGEKLVMRIFDPEVLVRSFADLGFSDDDQARW
                     KDMSERPNGIILVTGPTGSGKTTTLYSTLRQLATPAVNVCTIEDPIEMVEPAFNQMQV
                     QNVIDLGFAQGVRALMRQDPDIIMIGEIRDLETAEVAIQAALTGHLVLSTLHTNDAPS
                     AITRMLDLGVPSYLLSATVLGVMAQRLVRILCPHCKKVHQASPVEETMWDQLVAPWKS
                     NRPARFSRPVGCLDCRMTGYLGRVGLYEILMLSQDIKLAITEGKDIARIRDLAFREGM
                     KPLRISGAMKVAAGVTTLAEVFKVA"
     gene            complement(1889..>2316)
                     /locus_tag="PLL14_09005"
     CDS             complement(1889..>2316)
                     /locus_tag="PLL14_09005"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOG04107.1"
                     /translation="SFPSPASLAAAHYYAHRQNQFWRLLGVLLEEPLPEVDYVEKQRL
                     LLRHHIGVWDVYQQCLRQGALDAAIQSPAANDFLRLRNEAPGLRRVCFNGQTAGKFAG
                     WFQQQGYETRVLPSSSPAYTLAFESKLDLWRAAGLGEPR"
BASE COUNT          431 a          716 c          710 g          459 t
ORIGIN      
        1 gcgaccttga acacctcggc cagcgtggtg accccggcgg caactttcat cgcccccgag
       61 atccgcaggg gcttcatgcc ttcgcgaaaa gccaggtcgc gaatcctggc gatgtccttg
      121 ccttcagtga tcgccagttt gatgtcctgc gagagcatca gaatctcgta cagcccgacg
      181 cgcccgaggt atccggtcat gcggcagtcg agacagccca caggccgcga gaaacgcgcc
      241 gggcgattcg atttccaggg agcgaccagt tgatcccaca tcgtttcctc gaccgggctg
      301 gcctggtgca ccttcttgca gtgcgggcag aggatgcgta ccagccgctg cgccatcacc
      361 ccgagcaccg tggcactcaa cagatatgaa ggcacgccaa ggtcgagcat gcgcgtgatc
      421 gccgaaggtg cgtcgttggt gtgcagggtt gacaacacca ggtgaccggt cagtgccgcc
      481 tgaatcgcca cctcggccgt ctccagatcg cgtatttcac cgatcatgat gatatccggg
      541 tcctgccgca tcagcgcccg tacgccttgc gcaaagccga gatcgatgac gttctgcacc
      601 tgcatctggt tgaaggccgg ctcgaccatc tcgatcgggt cctcgatggt acagacattg
      661 accgccggtg tagccagttg cctcagtgtc gagtacagcg tggtcgtttt tccggatccg
      721 gtggggccgg tcaccagaat gatgccgttg ggtcgttccg acatgtcctt ccagcgtgcc
      781 tggtcgtcgt cgctgaagcc gagatcggcg aagctgcgca ccagcacttc cgggtcgaaa
      841 atgcgcatca cgagtttttc gccgaaggcc gtgggcagcg tcgacagacg cagttcggct
      901 tcctggccgt cggctgttcg cgtcttgatc cggccatcct gcggtcggcg tttctcgacg
      961 acatccatgc ggccgagaat cttgacacgg ttgaccattg ccgccatcac gctcatcgga
     1021 atctggtaaa cctgatgcag gacaccgtcg atgcggaagc gtacgatacc gatttcgcgc
     1081 cggggttcga tatgaatgtc gctggcgcgt tgttcgaaag catactgcca gagccagtcg
     1141 acgatattga cgatgtgctg gtcattggcg tcgaactggc gattggtccg gccgagttcg
     1201 accaactgct cgaacgatga aaggttccca ctcggccccc cgagttgtgc cgctttcttc
     1261 accgagcgcg ccagattgta gaactcgacc aggtagcgcg cgacgtcgac cgggttggcc
     1321 atcacccgtc ggatttcctt cttggcgatc gagcggatct ccttttccca gtcacgcagg
     1381 taaggttgcg tcgtcgccac gacgatctcc cgcgtcgtga cctgtatcgg cagaatgccg
     1441 aagcgtgtgg cataggcact cgacatgacg tcggttacgg ccgtgaagtc gaccttcagc
     1501 gggtcgatgt ggtggtaatc gagtcccacc ctgccggcca gccactcacc gagtccttcg
     1561 agattcaggg gtcgatgcgg ctcctgtgcc gacttccatt gctgatcggc gattacgacc
     1621 agcgggtgcg caacaaggcg ctgcagccgg ctttccttga gcagttgatc tgcgtccggc
     1681 gcgttgacca ggccatcgtc ggtcaaccag ccgaggactt cggccagggt cagacgatgt
     1741 tcatgaacga tattgaccgc tgcttccgac atgggccgtg ttccagagta atgaccggcc
     1801 actataccgg acaccgccaa ccgaaagcac gcgcgcggtg caggggtgca agaagagggc
     1861 cgcgccgaga cttctgcagc gccagcggtt accttggctc accaagtccg gccgcacgcc
     1921 agaggtccag cttgctttcg aaagccagag tatacgccgg gctgctcgat ggcaggaccc
     1981 gggtttcgta gccctgctgc tgaaaccagc cggcaaattt gccggccgtc tgaccattga
     2041 agcagacgcg ccgcaaaccc ggcgcctcgt tgcgcagtcg caagaaatcg ttggccgcgg
     2101 gagactggat cgctgcgtcg agcgcacctt gtcgcaggca ctgctgataa acgtcccaca
     2161 cgccgatgtg gtgccggagc agaaggcgtt gcttttcgac gtagtcgact tcgggcaagg
     2221 gctcctcaag cagcacgccg aggaggcgcc agaactggtt ttgccggtgc gcgtaatagt
     2281 gtgccgccgc cagcgatgcc ggcgacggaa aactgc
//
LOCUS       DAOSIT010000381         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_40031_length_2314_cov_11.331120, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000381 DAOSIT010000000
VERSION     DAOSIT010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40031_length_2314_cov_11.331120"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..498)
                     /gene="queF"
                     /locus_tag="PLL14_09010"
     CDS             complement(79..498)
                     /gene="queF"
                     /locus_tag="PLL14_09010"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOG04108.1"
                     /translation="MTTPPANILETFPNPAPQRDFHVHMEIPEFTCLCPKTGQPDFAT
                     LLLDYIPDETCVELKSLKLYIWSFRDTGCFHEDVTNRILDDLAGAIQPRYMRLTARFY
                     VRGGIFTTVVAEHRQAGWTPAPTVQLADFAGQSNTRG"
     gene            684..>2314
                     /locus_tag="PLL14_09015"
     CDS             684..>2314
                     /locus_tag="PLL14_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG04109.1"
                     /translation="MRLTKLKLAGFKSFVDPTTLALPGQLVGVVGPNGCGKSNVMDSV
                     RWVLGESKASELRGESMQDVIFNGSGTRKPVSRAAVELVFDNTLGRLSGQWSQYAELS
                     VKRLLTRNGQSEYYINNLHVRRKDVTDLFLGTGLGPRAYAIIGQGTISRIIEAKPDEL
                     RVFLEEAAGVTRYKERRKETENRLADTRENLARVEDIRLELGSQMERLEGQASVARQY
                     REYNDELTRKQQLQWLLRRNEAQSERARLAREVEQAGIDLEARNAALRDLETQLEEAR
                     EAHFAASDGVHAAQSDLFAANAEVARLEAEIRHRRESRREYETRLAQLTDEQRHWQAQ
                     AARLASDDEHWQGLLVSAEDRVEQGRCLLEACQERLPLAEDRHGAALEAVNQQRMAMA
                     QAEQRLHVEQANRSHAQRSLQIIASRRERLTLERQAMPAPDADEFADRQTELAELRTR
                     IGVLLESQQHQQLALPLHEQQRRAVLGEVQHAQKERAEANARRMALQQLQQRLQVNGK
                     LDDWLRRHRLQDGEPLWKSLHVEAGWEDAVEAVLR"
BASE COUNT          442 a          697 c          780 g          395 t
ORIGIN      
        1 gcctggtcca gtaccggttc gtcgagcagt tcctccaggc gctggaattg cgcctcggtc
       61 agtgatttct gcatgcgctc agcctcgggt attcgactgg ccggcgaaat cggcaagctg
      121 caccgtcggc gccggtgtcc agcccgcctg ccggtgctcg gcgacgaccg tggtgaaaat
      181 gccgccgcgt acgtagaagc gggcggtcag ccgcatgtag cgtggctgga tggcacccgc
      241 cagatcgtca aggatgcggt tggtgacatc ctcgtgaaag cagccggtgt cgcggaaaga
      301 ccagatgtag agcttcaggc ttttcagttc gacgcaggtt tcgtcgggga tgtagtcgag
      361 cagcagcgtc gcgaagtcgg gctggccggt ttttgggcac aggcaggtaa actcggggat
      421 ttccatatgc acatgaaaat cccgctgtgg cgccgggttg gggaaggttt cgagaatatt
      481 ggccggcggt gtggtcattg ttgttccgag cggtgtccgg ggagaacgaa tcgggcagac
      541 ggaatgctat cattggcacc tataatagca ttccgcgcga atatctgcgt gtcgtccgcc
      601 gccatgttat ggcctggcac tcgctcaata ccatgttttt gccgattttc tctggcgcgg
      661 cttaagcagt cgcgttctcg ttcatgcgcc tgaccaaact gaaactcgcc ggcttcaaat
      721 ccttcgtcga tccgacgacg ctggcgctgc ccggtcaact cgtcggcgtg gttggaccga
      781 atggttgcgg caaatccaat gtcatggatt cggtgcgctg ggtgctgggc gaatcaaagg
      841 cctcggagtt gcggggcgag tcgatgcagg atgtgatctt caatggttcc gggactcgca
      901 aaccggtgtc gcgagcggcg gtggagctgg tcttcgacaa taccctcggg cgcctctccg
      961 ggcaatggtc gcagtacgcc gaactgtcgg tcaagcgcct gctcacccgc aacggccagt
     1021 ccgagtacta catcaacaac ctgcacgtcc gtcgcaagga tgtgaccgac ctctttctcg
     1081 gcaccggtct gggaccgcgc gcctacgcga tcattggcca gggcaccatt tcgcgaatca
     1141 tcgaggccaa accggacgaa ctgcgcgtgt ttctcgaaga agccgctggc gtgacccgct
     1201 acaaggaaag gcgcaaggaa accgaaaacc gcctggccga cacgcgcgag aatctggccc
     1261 gtgtcgaaga catccgtctc gaactcggtt cccagatgga gcgcctcgag ggacaggcca
     1321 gtgtcgcccg ccagtatcgc gagtacaacg acgagttgac ccgcaagcag caattgcagt
     1381 ggctgctgcg acgcaatgag gcgcagtcgg agcgtgcgcg gctggcgcgt gaagtcgaac
     1441 aggcgggcat cgacctcgaa gcacgcaacg cggcgctgcg tgacctcgaa actcaactgg
     1501 aggaggcgcg cgaagcccat tttgctgcca gcgacggcgt gcatgcggcg cagagcgacc
     1561 tgttcgcggc caatgccgag gtggcgcgcc tggaagcgga gatccgtcat cgccgcgaat
     1621 cgcgacgcga gtatgagacg cggctggcac agttgaccga tgagcagcgg cattggcagg
     1681 cacaggcggc ccggctggcg agcgacgacg agcactggca ggggctgctg gtgtcggcgg
     1741 aggatcgcgt tgaacagggt cgctgccttc tcgaggcgtg ccaggaacgc ttgccgctgg
     1801 ccgaggacag gcacggtgct gcgctggagg ccgtcaatca gcagcgcatg gcgatggccc
     1861 aggccgagca gcgactgcac gtcgagcagg ccaaccgcag ccacgcgcag cgttcgctgc
     1921 agatcatcgc cagccgccgc gaacgcctga cgcttgagcg ccaggccatg ccggcccccg
     1981 acgccgacga gtttgccgac aggcaaacgg aactcgccga gttgcgcacg cgcatcggcg
     2041 tgctgctgga aagccagcag caccagcaac tggcgctgcc gcttcacgaa cagcagcggc
     2101 gcgcggtgct cggagaagtc cagcacgcgc agaaggaacg tgccgaggcg aatgctcgcc
     2161 ggatggcgct gcagcaactg cagcagcgcc tgcaggtcaa cggcaagctc gacgactggc
     2221 tgcgccggca tcgtctgcag gacggcgaac ccctgtggaa gtcgctgcac gtcgaagccg
     2281 gctgggaaga cgccgtcgaa gccgtcctgc gcga
//
LOCUS       DAOSIT010000382         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_40086_length_2311_cov_10.128989, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000382 DAOSIT010000000
VERSION     DAOSIT010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40086_length_2311_cov_10.128989"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..990
                     /locus_tag="PLL14_09020"
     CDS             13..990
                     /locus_tag="PLL14_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOG04110.1"
                     /translation="MSDLDIAERRNPQDGRFKVKALKKEVDIRVSILPTVHGEKVVMR
                     TLDKSNLAPSLASLGLDDFAYQAMKHAINQPHGIILVTGPTGSGKTTTLYSCLQDLNT
                     PDVNIVTVEDPVEYQIEGVNQVHVNQQTGLTFATVLRSVLRQDPDIVLVGEIRDDETA
                     DIAVKAALTGHLVLSTLHTNDAAGVIPRLIDMGVPPFLIASSLILAQAQRLYRKLCPV
                     CKKPATIEPDVLVANQIDPAFFENATLFEEVGCPKCNNIGFKGRGAFMEVLPVTDAIR
                     EAAVKGAPADVIRTVAIQEGMATLKDVGLMKVRDGITSLRAALEVTGSE"
     gene            1014..2099
                     /locus_tag="PLL14_09025"
     CDS             1014..2099
                     /locus_tag="PLL14_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG04111.1"
                     /translation="MSAKTSKIPTLRVQGVGKIIKKELIPFTRQTASMLNAGMSILTV
                     VSTLEDQCTHPGFKRVLRVLRESIESGMPFSDGLRHFPQIFDDMYLNMVAAGEKSGQF
                     AAVLRRLAAMLDASARLVRKVKSAMAYPTVVLSIALIIAGGLITFVVPVFTEMFAGFG
                     SALPGPTRMLVSASEFIRKYWYVLLPTVVVLIFMFRKWKASKAGRYQYDKLILKLPIF
                     GDLVQKVSIARFSRLFAQMLVSGVPILDAMQVVALSIGNKVIEESILAARAGIEQGNT
                     LSASLEGKPYLPILMIRMIAAGEKSGRIDEMLDSVADTYDDEVETTLAALTSLMEPLL
                     MVLLGLIIGSIVIAMYLPIFKLSTVIS"
BASE COUNT          465 a          711 c          645 g          490 t
ORIGIN      
        1 cgtatcaaga tcatgtcgga cctcgacatc gcggagcgcc gcaacccgca ggacggccgt
       61 ttcaaggtca aagccctcaa gaaagaggtc gatatccggg tcagcatcct gccgaccgtc
      121 catggcgaaa aggtggtgat gcgtacgctg gacaagagca atctggcgcc cagcctggcc
      181 tcgcttggtt tggacgattt tgcctatcag gccatgaagc atgccatcaa tcagccgcac
      241 ggcatcattc tggtgaccgg gccgaccggc tccggcaaga cgacaacgct ctactcttgt
      301 ctgcaagacc tgaacacgcc ggatgtcaac atcgtgacgg tcgaagatcc cgtcgagtat
      361 cagatcgagg gggtcaacca ggtgcacgtc aaccagcaga ccggactcac cttcgccacg
      421 gtcttgcgct ccgtgctgcg tcaagacccc gacatcgtgc tggtcggcga gatccgtgac
      481 gacgaaaccg ccgatatcgc cgtcaaggcg gcgctcaccg gccatcttgt gctgagcacc
      541 ctgcacacca acgacgccgc cggtgtcatt ccccgtctga tcgacatggg ggtcccgccc
      601 ttcttgatcg cctcctcgtt gatcctggcc caagcgcagc gcctctaccg caaactttgc
      661 ccggtctgta aaaagcccgc aacgatcgag cccgacgtgc tggtcgctaa ccagatcgat
      721 cccgcctttt ttgaaaacgc cacgctcttc gaagaggtcg gctgtcccaa gtgtaacaat
      781 atcggtttca aagggcgcgg tgccttcatg gaggtgctgc cggtcacgga cgccatccgt
      841 gaggccgccg tgaaaggtgc cccagccgat gtcatccgta cggttgccat ccaagagggc
      901 atggccacgc tgaaagacgt tggcctgatg aaagtccgcg atggcatcac ctccctgcgt
      961 gccgctctcg aagtcaccgg cagcgaataa tctttcagga aaggtccccc taaatgtccg
     1021 cgaaaacgtc taagattccc actctccgcg tccaaggtgt cggcaagatc attaagaagg
     1081 agttgatccc tttcacccgg cagaccgcca gcatgctgaa tgccggcatg tcgatcctga
     1141 cggttgtctc gaccctggaa gatcaatgca cacacccagg gttcaagcgc gtgctccgcg
     1201 tgctgcgcga gagtatcgag agcgggatgc ccttttctga cggcctgcgg cactttcccc
     1261 agatcttcga cgacatgtac ctcaatatgg tcgctgccgg cgagaagagc ggccagttcg
     1321 cggctgtgct cagacgcctg gccgccatgc tggacgcctc cgccaggctg gtgcgcaagg
     1381 tcaagtctgc catggcctac ccgaccgtcg tgctctcgat cgcgctgatc atcgccggtg
     1441 gcttgatcac cttcgtggtg ccggtcttca cggaaatgtt cgcggggttc ggttctgcgc
     1501 tgccggggcc gacccgcatg ctggtcagtg ccagcgaatt catccggaaa tactggtatg
     1561 tgctgcttcc taccgttgtg gtgctcatct tcatgttccg caagtggaag gcctccaagg
     1621 caggacgcta ccagtacgat aagcttatcc tcaagttgcc gatcttcggc gatctggtac
     1681 agaaggtttc catcgcgcgc ttcagccgcc tctttgcgca aatgttggtc agcggcgtac
     1741 cgattctcga cgccatgcag gtcgtggccc tttctatcgg taataaggtg atcgaagaat
     1801 cgatcctggc cgcccgcgcc ggcattgagc agggcaacac cctcagcgct tcactggagg
     1861 gcaagcccta cctgccgatc ctcatgattc gcatgatcgc tgccggcgag aagagcggcc
     1921 gcatcgatga gatgctcgac agcgtcgccg atacatacga cgatgaggtc gagactaccc
     1981 tcgccgccct gacgtcgctc atggagccgc tgctcatggt cctcctaggc ttgatcatcg
     2041 gtagcattgt gatcgccatg tacctgccga tcttcaagct cagtaccgtg attagctagg
     2101 ttctgcccgt ccttgccttt cacgccgttt ttccctataa tgaccacccg atggatacaa
     2161 atggacttgg agcctcggtt atgccgaaaa ttctgatcgt agatgacgaa gcgcgtattc
     2221 tgttactgtt gcagagtctg ctcaaggcga acggctatga tgtcgttacg gccaaggacg
     2281 gtaatggcgc gttggatgct ataaaaagtg a
//
LOCUS       DAOSIT010000383         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_40517_length_2293_cov_8.734138, whole genome shotgun sequence.
ACCESSION   DAOSIT010000383 DAOSIT010000000
VERSION     DAOSIT010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40517_length_2293_cov_8.734138"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..349)
                     /locus_tag="PLL14_09030"
     CDS             complement(113..349)
                     /locus_tag="PLL14_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04112.1"
                     /translation="MVLALQGAQALDSRLDDRVLPFCLFAHRFNATLVAARCCIQQLV
                     GDVELIKACALFGEDSLLSLEQTIQRADAGIEPG"
     gene            386..1087
                     /locus_tag="PLL14_09035"
     CDS             386..1087
                     /locus_tag="PLL14_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOG04113.1"
                     /translation="MQSETQRLEAAIRNVAAALADHQEATQDKFKRTHLAIAGLLLLT
                     VSGFALVKWMPAFAPASSESAIAKSDARITEISTQIAALSAGEAARQETAAMQQARID
                     QVSGKVSVLETSLGELRAAMRRMRVQGAGAGAVHDSRWILQQNPRAYTVQLVVSPSQA
                     DMARFIDRNVEQLALGSLAFSVTERDQRQQYNLFVGVFNTISQARAAIAALPAELRAN
                     RPWVRQFQSVQESLR"
     gene            1255..1965
                     /locus_tag="PLL14_09040"
     CDS             1255..1965
                     /locus_tag="PLL14_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOG04114.1"
                     /translation="MTANLTGTPAEGPLLPAVERQTGESPKCAIIWLHGLGADGHDFE
                     PIVDEFDFDQLPAIRFVFPHAPMRAVTINAGYVMRAWYDIVSIDFAPGREEEQGVRES
                     AEQIEALLVRENERGIPDARIVLAGFSQGGVIALHTGLRHRQRLAGILALSCYLPLTE
                     TLTAEAHPANREVPIFMAHGRGDTVIPYDFGKRSAKLLKTHGYPVEWHGYAAEHSVCA
                     EELQDIEAWLQQVLANAC"
     gene            1968..>2293
                     /locus_tag="PLL14_09045"
     CDS             1968..>2293
                     /locus_tag="PLL14_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG04115.1"
                     /translation="MLPLKYLAGYPEALQNQVREAIRHGRLAEMLHTRYPATHAVRSD
                     EALYAYALALKARFLRSAESPVKVVFDNHLLVTANALGSNGHVPRIQGKRIKSKREIR
                     VASVFK"
BASE COUNT          462 a          718 c          719 g          394 t
ORIGIN      
        1 tgccgagcaa cggatcgatg cggttcgcgg cgaagcacgc gcctggctgg ccgacacaga
       61 gcaacgctgg gacactcggc tcgcgagcaa caacgcgcgc atcagtacgg atttagcccg
      121 gctcgatgcc ggcatcggct cgctggatgg tctgttcaag gctcaggagc gaatcctcgc
      181 cgaacagcgc gcacgccttg atcagttcga cgtcacctac gagctgctgg atacagcaac
      241 gcgcggcaac aagagtcgca ttgaagcggt gcgcgaacaa gcagaaaggc aacacgcgat
      301 cgtcgaggcg cgaatcgagg gcttgggcgc cttgcagcgc gagcaccatg ccgaattgca
      361 gagcttgcag ggtctggttg gcgtcttgca gtcggagacg cagcggctgg aagcagcgat
      421 ccgcaacgtt gctgcggcac tggcggacca tcaggaggca acccaggaca agttcaagcg
      481 cacccatctc gccatcgccg gcctgctgct gctgaccgta tccggctttg ctctggtcaa
      541 atggatgccg gccttcgccc ctgcaagctc tgaatcggcg atcgcgaaaa gcgatgcccg
      601 aatcaccgaa atcagcactc agattgcggc actctcggca ggtgaggcgg ccagacagga
      661 aaccgcggca atgcaacaag cgcgcattga ccaggtatcg ggcaaggttt cggttctgga
      721 gaccagcctc ggcgaattgc gcgccgcgat gcggaggatg cgggtgcagg gcgcgggagc
      781 cggggcggta catgacagcc gctggatact tcagcaaaat cccagggctt ataccgttca
      841 gttggtggtg tccccgagcc aggccgacat ggcacgattc attgaccgca acgtggaaca
      901 gctcgctctg ggctccctgg ccttttccgt gaccgagcgc gaccagcgcc agcagtacaa
      961 cctgttcgtc ggcgtgttca ataccataag ccaggcgcgc gccgccattg ccgcgctgcc
     1021 ggctgaattg cgcgccaacc gaccgtgggt gcgccagttc cagtcggttc aggagtcttt
     1081 gcgttaaagg cacgactgcg gcaatacggt tggccaccgg gtggtggcca accgcttctt
     1141 tcgcctgcaa cgtcgggaac caccatccct tcgcgccagc gtggattctc gtgatactca
     1201 ctaccgggca gcctgctaga atggctgccc tcgacaataa caaggatcgg tcccatgact
     1261 gccaacctga ccggcactcc cgctgaaggg ccgttgcttc ctgccgtcga acggcaaacc
     1321 ggagagtccc cgaagtgcgc aatcatctgg ctgcatggcc tcggtgccga cggacacgac
     1381 ttcgagccca tcgtggacga gttcgatttc gaccagttgc cggcaatccg ctttgtcttc
     1441 ccgcatgcac ccatgcgggc ggtaaccatc aacgccggct acgtgatgcg cgcctggtac
     1501 gacatcgtct cgatcgactt tgcacccgga cgggaggagg agcagggagt acgcgagtcg
     1561 gccgaacaga tcgaagcgct gcttgtccgc gagaatgaac gcggtatccc cgatgcacgc
     1621 atcgtgctcg ccggattctc gcagggggga gtcatcgcct tgcacaccgg cttgcgacac
     1681 cgacagcgac tggccggcat cctggcgctc tcgtgctacc tgccactgac cgagacgctg
     1741 accgccgaag cgcatcccgc caaccgtgaa gtgccgatct tcatggcgca tgggcgtggc
     1801 gacacggtga ttccctacga tttcgggaag cgctcggcga aactgctgaa gacccacgga
     1861 taccccgttg agtggcacgg ttacgctgcg gaacacagcg tctgcgcgga ggaactgcag
     1921 gacatcgaag cctggttgca acaggtgctg gcaaacgcct gctgaacatg ctgccgctga
     1981 aatacctggc gggctatccc gaagcgcttc agaaccaggt ccgcgaggcc atcaggcacg
     2041 gccgtctggc ggagatgctg cacacccgct atccggcaac gcacgcggtg cgcagtgacg
     2101 aagcgctgta cgcctacgct ctggcgttga aggcgcgttt cctgcgcagt gccgaatcgc
     2161 cggtcaaggt cgtttttgac aaccacctgc tggtaacggc gaatgcactg gggagcaatg
     2221 gtcacgtgcc acgcatccag ggcaagcgca tcaagtcgaa acgcgagatc cgcgtggcca
     2281 gcgtgttcaa gga
//
LOCUS       DAOSIT010000384         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_40576_length_2290_cov_12.001342, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000384 DAOSIT010000000
VERSION     DAOSIT010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40576_length_2290_cov_12.001342"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="hslU"
                     /locus_tag="PLL14_09050"
     CDS             complement(<1..1058)
                     /gene="hslU"
                     /locus_tag="PLL14_09050"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOG04116.1"
                     /translation="MSSMTPQEIVCELDKHIVGQGKAKKAVAIALRSRWRRAQVAEPL
                     RQEITPKNILLIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGRDVETIIRD
                     LVEIAVKSGREQAMRGVRVRADDAAEDRILDALLPPARGNFFNDAQSTEDNATRQKFR
                     KKLREGELDDKEIEVEVAAPSMQAEIFAPPGMEELTAQIQGMFQNMGGGRKKSRKLKI
                     FEARRLLIEEEAAKLVNDEEVKLDAVRNVEQNGIVFLDEIDKIASRSDTHGVDVSRQG
                     VQRDLLPLVEGTTVSTKYGMIRTDHILFIASGAFHLAKPSDLIPELQGRLPIRVELDS
                     LSVADFECILTQTDACLT"
     gene            complement(1061..1597)
                     /gene="hslV"
                     /locus_tag="PLL14_09055"
     CDS             complement(1061..1597)
                     /gene="hslV"
                     /locus_tag="PLL14_09055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOG04117.1"
                     /translation="MDQYHGTTILSVRRGKRVAMGGDGQVTLGNIVIKASARKVRRIH
                     QGQILAGFAGGTADAFTLFERFEAKLDKHQGNLLRSAVELAKDWRSDRALRRLDAMLS
                     VADRENSLIITGNGDVVEPEHGIVAIGSGGAFAHSAARALVENSELDPAEVVRKSLQI
                     ASDLCIYTNQNFTIEVLE"
     gene            complement(1631..>2290)
                     /locus_tag="PLL14_09060"
     CDS             complement(1631..>2290)
                     /locus_tag="PLL14_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG04118.1"
                     /translation="GFEAVRQALAKNAHLRLDLSKVRKLDAAGCERLLELLQQAQRGK
                     REIELQGRDYIAALLDSRVVPGCPEDRPCWLLKLEFCQLRGQLEAFEEVAINYAVTFE
                     ISPPSWDPKRVQMAEPAPLMLAVDDQLVTEAYVIKGDVKGSRFGDLLAYAAANDPVLI
                     DCSAATRMDFLSAGALLNVLTTVRRTGRQIIFRHPNYLLAELFRVVGLKAVADVVLAR
                     S"
BASE COUNT          421 a          728 c          674 g          467 t
ORIGIN      
        1 ttggtcaggc aggcgtcggt ctgggtcagg atgcattcga agtcggccac cgacagcgag
       61 tccagttcga cgcggatcgg cagacggccc tgcaactcgg gaatgaggtc tgaaggtttg
      121 gccaggtgga aggcgccgct ggcgatgaac aggatgtgat cggtacggat catgccgtac
      181 ttggttgata cggtcgtgcc ttcaaccagc ggcaacaggt cgcgctgcac gccctgccgc
      241 gagacatcga cgccgtgcgt gtcgctgcgc gaggcgatct tgtcgatttc gtcgaggaag
      301 acgatgccgt tctgctcgac attcctgacc gcatcgagct tgacctcctc gtcattgacc
      361 agtttcgccg cttcctcctc gatcagcaac ctgcgcgcct cgaagatctt cagttttcgc
      421 gatttcttgc gaccaccgcc catgttctga aacatcccct ggatctgcgc ggtcaactcc
      481 tccattccgg gtggcgcaaa gatctcggcc tgcatcgatg gcgccgcaac ctcgacctcg
      541 atttccttgt cgtcaagttc gccttcgcgc aactttttac ggaatttctg gcgcgtggcg
      601 ttgtcttctg tcgattgtgc gtcgttgaag aaattgccgc gcgccggcgg cagcagcgcg
      661 tcgagaatgc gatcttcggc ggcgtcgtcg gcgcgcacac gcacaccgcg catggcttgt
      721 tcacgaccgc tcttgacggc gatctcgacc aggtcacgga tgatcgtctc gacatcgcgg
      781 cccacgtatc cgacctcggt aaacttggtc gcttcgacct tgatgaacgg agcattggcc
      841 agacgcgcca ggcggcgagc gatttcggtc ttgcccacac cggtgggtcc gatcagcagg
      901 atgttcttcg gggtaatctc ctgccgcaag ggctccgcca cctgtgcgcg ccgccaacga
      961 ctgcgcagtg cgatggccac ggccttcttg gccttgccct gaccgacgat atgtttgtca
     1021 agttcgcaaa cgatttcctg cggggtcatc gacgacatga ttattccagt acctcgatgg
     1081 tgaaattctg attggtatag atgcacagat cgctggcgat ctgcaaggac ttgcgcacca
     1141 cctcggcagg atcgagttcg ctgttctcga ccagagcgcg tgccgccgag tgcgcaaacg
     1201 ctcccccgga accaatggcc acaatgccgt gctccggttc caccacgtcc ccattgccag
     1261 tgatgatcag cgaattctcc cggtcagcca ccgacagcat ggcatccaga cggcgcaggg
     1321 cgcggtcgct ccgccagtcc ttggcgagtt caaccgccga gcgcagcaga ttgccctgat
     1381 gcttgtcgag cttggcttcg aagcgttcga agagggtaaa cgcgtctgcc gtgccgccag
     1441 caaagcctgc gaggatctgc ccctgatgaa tgcgccgaac cttgcgcgcg ctggccttga
     1501 tcacgatgtt gccaagcgtc acctgcccgt cgccgcccat ggcaacacgc ttgccgcggc
     1561 ggactgacag aatcgtcgtg ccatggtact gatccatgcg aactcctgtt gatgtgcctg
     1621 tctggagtcc ctaactcctg gcgagcacga catcggcgac ggctttcagc ccgacaacgc
     1681 ggaacaactc ggcaagcagg tagttggggt gacgaaagat gatctggcgg cccgttcgcc
     1741 tgaccgtggt caacacgttg agcaaggcac cggcgctgag gaagtccatg cgcgtcgcag
     1801 ccgagcagtc gatcagcacc ggatcgttgg ccgcagcgta cgccagcaga tcgccgaaac
     1861 gggaaccctt gacatcgcct ttgatgacat acgcctcggt caccaattga tcatcgacag
     1921 ccagcatcaa tggggcgggc tcggccatct gcacccgctt ggggtcccag gacggcggcg
     1981 aaatctcgaa cgtcacggca tagttgatcg ccacttcctc gaaggcctcc agttgccctc
     2041 gcaactggca aaactcgagc ttcaacagcc agcagggtcg gtcttccggg caccccggga
     2101 cgacccgact gtccagcagg gcggcaatgt aatcgcgccc ctgcaattcg atttcgcgct
     2161 tgcctcgctg tgcttgctgg agtagttcca gaagtcgctc gcaaccggcg gcatccagct
     2221 tgcgcacctt ggacaggtcg agacgcaggt gcgcgttctt cgcgagtgcc tgacgcaccg
     2281 cttcgaaacc
//
LOCUS       DAOSIT010000385         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_40784_length_2281_cov_8.435310, whole genome shotgun sequence.
ACCESSION   DAOSIT010000385 DAOSIT010000000
VERSION     DAOSIT010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40784_length_2281_cov_8.435310"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..296)
                     /locus_tag="PLL14_09065"
     CDS             complement(117..296)
                     /locus_tag="PLL14_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04119.1"
                     /translation="MPIDLAIAAARAATDVLSPVGICYHCDAIVPPGCRFCDADCRDD
                     HERQQRADKIAGRRP"
     gene            complement(332..691)
                     /locus_tag="PLL14_09070"
     CDS             complement(332..691)
                     /locus_tag="PLL14_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG04120.1"
                     /translation="MREVIRAAGGSKVVGCKLWPSLPVDQAASKISDCLNPDRRQHFN
                     EAELLHLLRIGREIDCHAAMHYVASICGYSQPDPVTPADEVQALQQQFILATKELKAM
                     SQRLEAITARANLTAVR"
     gene            complement(982..1218)
                     /locus_tag="PLL14_09075"
     CDS             complement(982..1218)
                     /locus_tag="PLL14_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG04121.1"
                     /translation="MITSPPIYEFVMAKLRAKTIPQRTVAAGSGVPYSTLTKIAQGSI
                     KDPSVHHVQRLADYFAKQAPASQADHVEQQQSAA"
     gene            1211..1693
                     /locus_tag="PLL14_09080"
     CDS             1211..1693
                     /locus_tag="PLL14_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04122.1"
                     /translation="MIMVAHPIPICGSAQYPHIDLRICDYPQMAIAQIISGNLIRLMA
                     SYPGRDTLGKVAKAAGVGFGTVQRARNADGNLTVQNLDLIAKAFRRSAKDLLVDPDEA
                     YGPAAPVTVLAVHEPPIDERELLQGYRDASQEVREILLDLARKASRKKDFGPRSERND
                     "
     gene            1756..2109
                     /locus_tag="PLL14_09085"
     CDS             1756..2109
                     /locus_tag="PLL14_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04123.1"
                     /translation="MDSQTPPPRRSRLAPDQGFPAGNFPAQEEAPAVDVRTADGRAAE
                     TLGRIEAAPGLPASGQPDDSPQRVIVTDVHMAFWSMVSLLVKLAFALIPAAVIITLLV
                     LVTISVAERLSSLVR"
BASE COUNT          383 a          651 c          780 g          467 t
ORIGIN      
        1 cttcgcatta cccgcagtca gaagcgctgg gtaggacccg ctgtccggct gtggggagtt
       61 gcgttgggtt gcgtgcgggt tgcgcgcgcg gtggatttcg ctggggtggg tggggttcat
      121 gggcgtcggc cggcgatctt gtcggcgcgc tgctggcgtt cgtggtcgtc gcgacaatcg
      181 gcgtcgcaga agcggcagcc gggcgggacg atggcgtcgc agtggtagca gatgccaacg
      241 ggtgagagga cgtcggtcgc ggctcgggcg gcggctatcg ccaggtcgat cggcatttgc
      301 tctgcttcct tggcgcggtc agcgtcgtcc atcagcggac tgccgtgaga ttggcgcggg
      361 ccgtgatggc ttccaagcgc tggctcatgg cttttagttc cttggtggcc aggatgaact
      421 gctgctgcaa ggcttgcacc tcgtcggctg gcgttacggg gtccggctgg gagtagccac
      481 agatcgatgc gacgtagtgc attgcggcat ggcagtcgat ctcgcggccg atgcgcagca
      541 ggtgcagcag ttccgcctcg ttgaagtgct ggcggcggtc tgggttgagg cagtcgctga
      601 tcttgctcgc ggcctgatca acggggagcg atggccagag cttgcagccg accaccttgg
      661 agccgccggc ggcgcggatg acttcgcgca aagcgtcatg gattgattcg tggaacaagg
      721 cctgttgcat ggtcggtcct ttcgtggctt gtacgtggct gtacgaaaaa tccggacagc
      781 cttcgtacag gtggcgggct atcgtttatc cgtgccggac agatcggcca gatcggtgcc
      841 tggccttctc gagtcgttga gcacgcgacg tgtccttggt gaaaaaaagc cctccccgcg
      901 tctgtcagga cgcggagagg gacagaagcc gcacaggcgc ggagagttcg cctggcggtg
      961 ggaggagacg ggttgtgggc gtcatgcggc ggactgctgt tgctcgacgt ggtcggcctg
     1021 actggccggc gcctgcttgg cgaagtagtc ggcgaggcgc tgcacgtggt gcacgctcgg
     1081 gtctttgatg ctgccctgcg cgatctttgt cagggtcgag taggggacgc cactgccggc
     1141 agcaaccgtg cgctggggta tggtcttggc ccgtagtttg gccatcacga attcgtagat
     1201 tggtggagag gtgatcatgg tcgcgcatcc tatacccata tgtgggtcag cgcaataccc
     1261 acacattgat ttgcgcattt gcgactatcc gcaaatggct attgcgcaaa tcatcagcgg
     1321 caacttgatc aggctgatgg catcctatcc aggccgcgac acgctcggga aagtggccaa
     1381 agctgccggc gtcgggtttg gcaccgttca gcgcgcgcgc aatgcggatg gcaatctgac
     1441 cgtccagaat ctcgatctga ttgccaaggc ctttcggcgc tcggccaagg acttgctcgt
     1501 tgatccggat gaggcgtatg ggccagcggc cccggtaacg gtcctggcgg tgcatgagcc
     1561 tccgattgat gagcgcgagc tcctgcaagg ctatcgagac gcctcgcagg aggtgcgaga
     1621 gatactgctt gacctagcca gaaaggcgtc gcgcaagaag gatttcgggc cgcgcagcga
     1681 gagaaacgac taacccggac tcgactgcgc gcgatcaagg gctgcaaatg tcacgaggag
     1741 tgatatcgtt ccgccatgga tagccagaca ccccccccaa ggcgctcccg gcttgctcca
     1801 gatcagggct tcccggcggg caatttcccg gctcaggaag aggcgccagc cgtcgacgtc
     1861 cggaccgcgg acggcagggc tgcggagacg ctcgggcgaa tcgaggcggc gccggggttg
     1921 ccggcttctg ggcagccaga cgacagcccg cagcgcgtca tcgtcaccga cgttcacatg
     1981 gccttctggt cgatggtctc gctgcttgtc aagctggcgt tcgccctcat tcccgcggcg
     2041 gtcatcatca ccttgcttgt gctcgtcacc atttccgtag ccgagcggtt gagcagcctg
     2101 gtccggtagc aatcgccagg ccgttggaag gagcccgtct gtaaggcggc ttttttttcg
     2161 cttgaactac ccatttacgg attgacacga cacccataaa cgggtattgt gtcattcatc
     2221 aaccaaacac ggagccacca catgccccac atcgatcaac ccggcggcgt tcttcttgcc
     2281 g
//
LOCUS       DAOSIT010000386         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41001_length_2272_cov_10.831304, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000386 DAOSIT010000000
VERSION     DAOSIT010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41001_length_2272_cov_10.831304"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="lolA"
                     /locus_tag="PLL14_09090"
     CDS             complement(<1..527)
                     /gene="lolA"
                     /locus_tag="PLL14_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOG04124.1"
                     /translation="MIRRGLFAAACLLAAQVAGAGAIDKLHQFLESTRTVRADFTQTV
                     IARNGRQAQTSSGVMMFSRPGKFRWQIEKPYSQLLLGDGEKVWIHDPDLRQVTVRKAG
                     TALGGTPASLLAGDTQIEKSFSLREAGERDGMEWIEAIPKVQDSGFEKVRLGFAGNDL
                     RAMELFDSLGQITSLA"
     gene            complement(524..>2272)
                     /locus_tag="PLL14_09095"
     CDS             complement(524..>2272)
                     /locus_tag="PLL14_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG04125.1"
                     /translation="PKQGIKRSAAREPTLAEGPQPSLADRPEPAATLAPLPPQDEQPV
                     EAPQPDPPPSEKAGAITTPPLALKVDKHIARERQPALFPDAVEGALLPPLHLLEPAPP
                     QSDRVRPETLEYTSRLIERKLADFGVQVTVTAAYPGPVITRYEFEPAVGVKGAQILNL
                     AKDLARSLSLTSVRVVETVPGKSSMAIELPNPKRQVVRLLEIISSKEYHELNSPLTIT
                     LGKDISGQPLVVDLAKMPHLLVAGTTGSGKSVGINAMILSLLYKAEPDQVRLILVDPK
                     MLELSIYEGIPHLLAPVVVDMKQAANALNWCVAEMEKRYKLMSAMGVRNLSGLNQRIR
                     DAEKSGEKIANPVSLTPDAPEPLSILPYIVVVIDELADLMMVSGKTVEQLIARLAQKA
                     RASGIHLILATQRPSVDVITGLIKANIPTRISFQVSSKIDSRTILDQMGAETLLGQGD
                     MLYLAPGTGYPTRVHGAFVADDEVHRVVDFLKKTGTPDYVDGVLKGGVGDEDGEGGGE
                     NAGLDTDGEADPVYDQAVEVVLKNRRASISLVQRHLRIGYNRSARLIEAMEKAGLVSA
                     MDARGGREVLARKEEE"
BASE COUNT          369 a          714 c          735 g          454 t
ORIGIN      
        1 gccagcgagg tgatctggcc caggctgtcg aacaactcca tcgcccgtag atcgttgccg
       61 gcaaaaccga gtcggacctt ctcgaaaccg ctgtcctgga ccttgggaat cgcttcgatc
      121 cattccatgc catcgcgttc gccggcttcg cgcaggctga agctcttttc gatctgggta
      181 tcgccggcga gcagcgaggc cggcgttccg ccgagcgcgg taccggcttt cctgaccgtg
      241 acctggcgca aatccgggtc gtggatccag actttttcac cgtcccccag cagcaactgg
      301 ctgtagggct tctcgatctg ccagcgaaac ttgccggggc gcgagaacat catgaccccg
      361 ctcgaggtct gcgcctggcg cccgttcctg gcgataacgg tctgcgtgaa gtcggcacgc
      421 accgtccggg tcgattccag gaactggtgc aacttgtcga tcgcccccgc gcctgccacc
      481 tgcgcagcca gcaggcaggc ggctgcgaag aggccgcgcc ggatcattct tcctccttgc
      541 gggccaggac ttcgcgacca ccacgcgcat ccatggcgga aaccagtcca gccttctcca
      601 tcgcctcgat caggcgggcc gagcggttgt agccgatgcg caggtgccgc tgcaccagtg
      661 agatcgacgc gcgtcgattc ttcagcacca cctcgaccgc ctggtcatag accggatcgg
      721 cctccccatc ggtgtccagg ccagcattct cgccgccacc ttcgccgtcc tcatctccga
      781 cgccgccctt gagcacgcca tcgacgtaat ccggggtacc cgttttcttc aggaagtcga
      841 cgactcgatg cacttcgtca tcggcgacaa aagcaccatg gacgcgggtc ggatagcccg
      901 ttcccggtgc cagatagagc atgtcgccct ggccaagcag cgtctcggca cccatttggt
      961 cgaggatggt gcgcgagtcg atttttgacg acacctggaa tgagatacgc gtcgggatgt
     1021 tggccttgat caggccggtg atgacgtcga ccgacggtcg ctgtgtggcg agaatcaggt
     1081 gaatgcccga ggcgcgggcc ttttgggcca ggcgggcaat cagttgctcg acggtcttgc
     1141 cggaaaccat catcaggtcg gcgagttcgt cgatcacgac gacgatgtac ggtagtatcg
     1201 acagcggttc gggagcgtcc ggggtcagcg ataccgggtt ggcgattttc tcgccgctct
     1261 tctcagcgtc acggatgcgc tggttgagtc cggacagatt gcgcaccccc atcgcggaca
     1321 tcagcttgta acgcttttcc atctcggcaa cgcaccagtt aagggcgttg gcggcttgtt
     1381 tcatgtcgac caccacgggt gcgagcaggt gcgggatgcc ctcgtagatc gacagttcga
     1441 gcattttggg gtcgaccagg atcagccgta cctggtcggg ctcggccttg tagagcagcg
     1501 acaggatcat cgcgttgata ccgaccgact tgccggagcc ggttgtgcca gctaccagca
     1561 ggtgcggcat cttggccaga tcgaccacca gcggctggcc gctgatgtcc ttgccgaggg
     1621 tgatcgtcag cggcgagttc agttcatggt attccttgct ggagatgatt tccagcaagc
     1681 gcaccacttg acgcttcggg ttcgggagtt cgatcgccat cgacgacttg cccggcaccg
     1741 tttcgacgac gcgtaccgag gtcagcgaca gcgagcgcgc cagatccttg gccaggttga
     1801 ggatctgcgc ccccttgacg cccaccgccg gctcgaattc atagcgggtg atgaccggcc
     1861 ccgggtaggc cgcggtcacc gtgacctgca caccaaagtc ggcgagtttg cgctcgatca
     1921 gccgcgaggt gtattcaagg gtttccgggc gtacgcggtc actctgcggt ggtgccggtt
     1981 cgagcagatg caggggcggc agcagggcac cttcgacggc gtcgggaaag agcgctggtt
     2041 gtcgctcgcg ggcgatgtgc ttgtcgactt tcagcgcaag aggcggtgtc gtgattgcgc
     2101 ctgctttctc actcggtgga ggatccggct gcggggcttc gacaggctgc tcgtcttgcg
     2161 gcggcagcgg tgccagggtg gcagccggct cgggcctgtc ggcgagggac ggctgcggcc
     2221 cttcggcgag cgttggctcc cgagcggcgc tgcgcttgat cccctgcttc gg
//
LOCUS       DAOSIT010000387         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41347_length_2257_cov_7.945504, whole genome shotgun sequence.
ACCESSION   DAOSIT010000387 DAOSIT010000000
VERSION     DAOSIT010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41347_length_2257_cov_7.945504"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1327)
                     /locus_tag="PLL14_09100"
     CDS             complement(134..1327)
                     /locus_tag="PLL14_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG04126.1"
                     /translation="MLSVNRDAGTIPPAIWALGFVSLLMDVSSELIHSLLPVFLMTAL
                     GVSALTIGLIEGAAEATALIVKVFSGVLSDWWGKRKPLALLGYGLGAIAKPLFALAGG
                     VGLVVAARLIDRVGKGIRGAPRDALVADLAPPGIRGAAFGLRQSLDTVGAFLGPLLAM
                     GLMLACADDFRAVFWVATLPAGLAVALLFFGVREPAGAQVARRVNPIRSENLRRLSGD
                     YWWVVGIGAACTLARFSEAFLILRAAQGGLPVALTPLVLIGMNLIYAASAYPFGKLSD
                     RLSHAGLLAWGLVVLIAADALLAYSNHWAWVWAGISLWGLHLGMTQGLLATMVAATAP
                     ADLRGTAYGFFNLVSGLAMLLASALAGLLWDRAGAPFTFLAGAGFSAMALMAMVIRSA
                     RRTAS"
     gene            complement(1361..>2257)
                     /locus_tag="PLL14_09105"
     CDS             complement(1361..>2257)
                     /locus_tag="PLL14_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HOG04127.1"
                     /translation="FDFYDGGGLDMACLGMAECDATGNVGVSRYGGRLTGAGGFINIS
                     QNARLVVFVGTFTGSGLKVEVVDGKLRIIEEGRQRKFLKQVEQITFNGQYAYDRGKPV
                     YYITERCVFRRVRRGLALIEVAPGIDVERDILPYMAFEPIIGEYGEMDARIFNTNPMG
                     LEAELLNLSLPERVIYDPERNILFLNFEGMHVRGADDVKAIWDICELRCRAAGKRVGV
                     IINYDRFRINQDMYDAYAEMDRYFLANYFSQITRYATSAFLRSKLGEAFSARSIAPHI
                     FERREEAQAFLAGRNGDSGRRA"
BASE COUNT          412 a          777 c          735 g          333 t
ORIGIN      
        1 atctgtactt cgacaccgtg gcgcatgtcc tctacggcaa caccgacgcc gatacggcgg
       61 cggaattcgc gattcagctc gtcggggtca ccaccctgac cgctgttgat ctattcctct
      121 gactccgcca cgttcatgac gctgtgcgcc gcgcgctgcg gatgaccatc gccatcaggg
      181 ccatggcgct gaagcctgcg ccggcgagaa aggtgaatgg ggccccggcg cggtcccaga
      241 gcagcccggc caaggcgctg gccagcagca tggcgagtcc gctgacgaga ttgaagaagc
      301 cataggcggt gccgcgcaga tcggccggcg ccgtggccgc caccatcgtc gccagcaagc
      361 cctgggtcat gcccaggtgc aggccccaga gggagatgcc ggcccatacc caggcccagt
      421 ggttgctgta cgccaggagc gcatcggccg cgatcagcac gaccagtccc caggccagca
      481 gcccggcgtg ggacaggcgg tccgacagct tcccgaaggg gtaggcactg gccgcgtaga
      541 tcagattcat gccgatcagc accagcggcg tcagggcgac aggcagcccg ccctgcgcgg
      601 cgcgcaggat cagaaaggct tccgaaaacc gcgccagcgt acatgccgcg ccgatgccca
      661 ccacccacca gtagtcgccc gagaggcgac gcaggttctc gctacggatc gggttcacac
      721 gtctggcgac ctgcgcgccc gcaggctcgc gcacgccgaa gaacaacagc gcgacagcga
      781 ggcctgccgg gagggtggcc acccagaaca cggcccgaaa atcgtcggcg caggcgagca
      841 tcaaacccat cgccagcagc ggcccgagga aggcgcccac ggtgtcgagc gactggcgca
      901 gaccgaatgc cgccccgcgt atgccgggcg gcgcgaggtc ggccaccagc gcatcgcgcg
      961 gcgcgccccg gatgcccttg ccgacgcggt cgatgagccg ggcggccacc accagcccga
     1021 cacccccggc cagcgcgaac agcggtttgg cgattgcccc caggccgtaa ccgagcaggg
     1081 ccagcggctt gcgcttgccc caccagtcgg agagcacgcc cgagaagacc ttcacgatca
     1141 acgccgtcgc ttccgccgcg ccctcgatca ggccgatcgt cagcgcgctg acgccgagag
     1201 ccgtcatcag gaacaccggc agcaggctgt ggatcagttc cgaagacaca tccatcagca
     1261 ggctgacaaa gcccagcgcc cagatcgcgg ggggaatcgt gccggcgtcg cggttcacgg
     1321 acagcacggc ggttcctctg tgtcggggtc gttcaccggg tcaggcgcgg cggccgctgt
     1381 cgccgttgcg cccggccagg aacgcctgcg cttcttcacg gcgctcgaag atatgcggcg
     1441 cgatgctgcg cgccgagaac gcctcgccga gcttggaacg caggaaagcg ctggtggcgt
     1501 agcgggtgat ctgactgaag taattggcca gaaagtaacg gtccatctcc gcataggcgt
     1561 cgtacatgtc ctggttgatg cgaaaacggt cgtagttgat gatcaccccg acgcgcttgc
     1621 cggccgcccg gcagcgcagt tcgcagatat cccagatcgc cttcacgtcg tcggcgccgc
     1681 gcacgtgcat gccctcgaaa ttgaggaaca ggatgttgcg ctccggatcg tagatcaccc
     1741 gttccggcaa ggacaggttg agcagttccg cctccaggcc catcgggttg gtattgaaga
     1801 tgcgcgcgtc catttcgcca tattcgccga tgatcggctc gaacgccatg tacggcagga
     1861 tgtcccgttc gacgtcgatg cccggcgcca cctcgatcag cgccagcccc cgccgcaccc
     1921 ggcggaagac gcagcgttcg gtgatgtaat acaccggctt gccgcgatcg taggcgtact
     1981 ggccgttgaa ggtgatctgc tcgacctgct tgaggaactt ccgctgccgg ccctcttcga
     2041 tgatccgcag cttgccgtca acgacctcga ccttgagacc gctgccggtg aaggtgccga
     2101 cgaagaccac caggcgggcg ttctggctga tgttgatgaa gccgccggcg ccggtcaggc
     2161 ggccgccgta gcggctgacg ccgacgttgc cggtggcatc gcactcggcc atgcccagac
     2221 acgccatgtc gaggccgcca ccgtcgtaga aatcgaa
//
LOCUS       DAOSIT010000388         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41437_length_2254_cov_9.775352, whole genome shotgun sequence.
ACCESSION   DAOSIT010000388 DAOSIT010000000
VERSION     DAOSIT010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41437_length_2254_cov_9.775352"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLL14_09110"
     CDS             complement(<1..1229)
                     /locus_tag="PLL14_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOG04128.1"
                     /translation="MSRLLLFTIVFIEGFCSLGAEVIALRQIIPHLGSSIVVTAPTIG
                     FFLLALALGYHAGAKIDEGYIEVVAKNFLLAALLVGIGLAGIGVDLLFTALRPPELAY
                     LILVAGVLCPIAWLLGQTVPILTNLLLRERAGEASGEALYYSTLGSFLGSLSLSLLVM
                     QWLGVSAAVALCALLLVIGYTLLRGPDWRTAVISLAVAGLTLVANAWLRPEIETAYAS
                     YLVLPVQYEGAINGRALKNNSSFASLIDDSEPPLYARYIRHIRSLLLDELGFRERRIL
                     VLGAGGFTLSHREPSNHYTYVDIDPAVRGIAEKHFLGEAARGEFIADDARRFVIRSEQ
                     RFAAAIVDVYGSHSSIPGHLVTREFWLATRRVLEPEGWLIANLILDSALATPYSRNLL
                     ATIESVYGRCAVEVLQK"
     gene            complement(1319..>2254)
                     /locus_tag="PLL14_09115"
     CDS             complement(1319..>2254)
                     /locus_tag="PLL14_09115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG04129.1"
                     /translation="GRRVLRTPLLKATSMCDKPMPRDLAGWVTFLSSAEMPILRQTAR
                     RLDHARQQIDRVNGRDITDIVLQDPLLAIRVLAYIQEASHRRLQSDITNIASAVMMLG
                     IDPFFRKFDQPRTLETMLSGQPQALLGVVQVILRTQRASHYAHEWAFARHDMNVEEVA
                     LAALLNDIAEILLWCFAPHLAIEIRSRLQADRALRSASAQQEVLGFPLADLQLALCHA
                     WHLPELLTTLMDDGKAHLQRVQNVKLAVNLARHSTGGWTDPAIPDDLTAVQNLLHISR
                     ETLLHRLQLPPEVLPGGLSEDAAGGQGGWPEQPRH"
BASE COUNT          423 a          689 c          773 g          369 t
ORIGIN      
        1 tttttctgca ggacctcgac ggcgcagcga ccatagacgc tctcgatggt cgccagcaga
       61 ttgcgcgaat agggtgtggc gagggcgctg tccaggatca ggttggcaat cagccatccc
      121 tcgggttcca gcacccgccg cgtcgccagc cagaattcgc gagtgaccag gtggccgggg
      181 attgacgagt gcgagccata gacgtccacg atcgccgcgg caaagcgctg ttcgctgcgg
      241 atcacgaaac ggcgcgcgtc gtcggcgatg aactcgccgc gcgccgcttc gccaaggaaa
      301 tgcttttcgg caatgccgcg gaccgccggg tcaatgtcga cataggtgta gtgattactg
      361 ggttcgcggt gtgaaagcgt gaagccgccg gcaccgagaa ccaggatccg gcgctcacgg
      421 aaacccagtt catcgagtag caggctgcgg atgtgccgga tatagcgggc gtagagcggt
      481 ggctcggagt cgtcgatcaa cgaggcgaag gaactgttgt tcttcagggc gcgcccgttg
      541 atcgcgcctt cgtattgcac cggcaacacc agatagctgg cgtaggcggt ttcgatttcg
      601 ggacgcagcc aggcattggc gacgagggtc agaccggcga cggccaggct gatcaccgcc
      661 gttcgccagt cgggtccgcg cagcagcgtg tatccgatga ccagcaacag ggcgcagagt
      721 gcgacggctg ccgaaacgcc cagccattgc atgaccagca gtgacagcga cagcgaaccg
      781 aggaaggaac cgagggtcga gtagtacagc gcttcgccgc tggcctcgcc ggcgcgttcg
      841 cgaagcagca ggttggtcag aatcggcacg gtttgcccga gcagccaggc gatcgggcag
      901 agcacgccgg cgacgaggat caggtacgcg agttccggcg gccgcagggc cgtgaacagc
      961 aggtcaacgc caatccccgc cagcccgatg ccgacgagca gcgcggcgag caggaagttc
     1021 ttcgccacca cctcgatata cccctcgtcg atcttcgcgc cggcgtggta acccagcgcc
     1081 agggcgagca gaaagaagcc gatggttggc gcggtcacca cgatcgaact gcccaggtgc
     1141 ggaatgatct gacgcagcgc gatgacctcg gcgccgagcg agcagaaccc ttcaatgaag
     1201 acaatggtaa acagcaggag gcgtgacatg ggcagggggg gcgtggcgcc ggcggaggtg
     1261 agccggcact ataccccagc acggtcacgg tccggcgctc acccgggcgc cgccgcgctt
     1321 agtggcgggg ctgctccggc cagccgccct ggccgccggc cgcgtcttcg ctgaggcctc
     1381 ccggcaggac ttccggtggc aattgcaggc ggtgcagcaa ggtttcgcgg ctgatgtgca
     1441 acagattctg gacggccgtg aggtcgtccg gtatcgccgg atcggtccag ccaccggtcg
     1501 aatggcgggc caggtttacg gccagcttca cattctgcac tctttgcagg tgggccttgc
     1561 cgtcgtccat cagggtggtc agcaactccg gcaggtgcca ggcatggcag agcgccaact
     1621 gcaggtcagc aagcggaaaa ccgaggacct cctgttgggc actggcgctg cgcagcgccc
     1681 ggtcggcctg cagccggctg cgaatctcga tggccagatg cggtgcgaag caccacagca
     1741 ggatctcggc gatgtcgttc agcagtgccg caagagccac ttcctcgacg ttcatgtcgt
     1801 gtcgcgcaaa cgcccactca tgcgcatagt gcgatgcccg ctgggtgcgc agaatcacct
     1861 gcacgacgcc aagcaaggct tgcggctgac cactgagcat ggtttcgagc gtgcgcggct
     1921 ggtcgaactt gcggaaaaac gggtcgatgc cgagcatcat gaccgcgctg gcgatgttcg
     1981 tgatgtcact ctgcaggcgg cggtggctgg cttcctgaat gtaggccagc acgcgaatgg
     2041 ccagcagcgg atcctgcagc acgatatcgg tgatgtcgcg gccgttgacc cggtcgattt
     2101 gctggcgggc gtggtcgagg cgacgcgcag tctggcgcag aatcggcatc tccgcgctgc
     2161 tcaggaatgt cacccagcct gcgaggtctc ttggcattgg cttgtcgcac atggatgtcg
     2221 ccttcaacaa gggcgtccga agaacgcgcc ggcc
//
LOCUS       DAOSIT010000389         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41458_length_2253_cov_7.306642, whole genome shotgun sequence.
ACCESSION   DAOSIT010000389 DAOSIT010000000
VERSION     DAOSIT010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41458_length_2253_cov_7.306642"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..546)
                     /locus_tag="PLL14_09120"
     CDS             complement(214..546)
                     /locus_tag="PLL14_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOG04130.1"
                     /translation="MPNEINFLDITLLGKKYRVACPPDQHAALLNAAAYVDGKMRDIA
                     EKTKSTLAERIAVMTALNIAHEHLGLNQEAAQQNQKNEFEIGLDMDAVKRRIFSMEAE
                     LESVLKAE"
     gene            complement(560..760)
                     /locus_tag="PLL14_09125"
     CDS             complement(560..760)
                     /locus_tag="PLL14_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04131.1"
                     /translation="MLNELNTLESKISQVVALCHSLRTENDGLRKQLAAAEAENERLA
                     ERMAAARSRIEQLAELLPEFKA"
     regulatory      910..1122
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1248..>2253
                     /locus_tag="PLL14_09130"
     CDS             1248..>2253
                     /locus_tag="PLL14_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOG04132.1"
                     /translation="MYPNPSRAAAATTFAASVAALSSTAPHAADQQLDSVVVTATRQA
                     TRSNELTSDVSVITRAEIDQAGESTLAQLLARQPGVQYVANGGAGTNSGVFIRGASTN
                     QSIVLIDGQRIGSATTGSAALSRIPLDQIERIEILRGPASSLYGPDAIGGVIQIFTRG
                     GEGPTRVNASSGYGSYNTTDTSVGVAGGTDVVSFSVQAGYLDTDGFNASNNRRNVSYN
                     RDRDGYYNKNLSANLSVRPARGQEIGVNLLASSGTNRYDANDFSALGAAKNYSSDQNV
                     GSYSIYSRNRLHSAWTSTLRLGRSTDASKDYVGNLRDATFRTDMDLLSWQNDLRLPVG
                     E"
BASE COUNT          449 a          653 c          663 g          488 t
ORIGIN      
        1 ttttgtcccc gcgagtgccg tcaggccggc atcctgaacc tgggttcagt taggtcactt
       61 cccttccgca tcaggtactc ccgactgggg gggcacacaa cagcggtttc tatggtccgc
      121 aaccaatgcc aatctacatt ggttcaagga atttatgacc ttaacaaaca ccgcagggaa
      181 ctctcttgga agccaaaaac agtgatgcaa actctattca gctttcagca ccgactccaa
      241 ctctgcctcc atggaaaaga ttctacgctt aacggcgtcc atgtccagcc ctatttcgaa
      301 ttcatttttc tgattctgtt gtgctgcttc ctgattgagc ccaaggtgct cgtgggcgat
      361 gttgagcgcc gtcatgactg caattcgctc ggctagagtg ctcttcgtct tctccgcgat
      421 gtcgcgcatt ttgccatcaa cataggccgc agcgttaagc aacgccgcgt gttgatcagg
      481 tggacaggca acgcgatatt tcttgccaag cagggtgatg tcgagaaaat tgatttcgtt
      541 gggcatcagg taagaatttt caggccttga attcgggcaa tagttcggcc agttgttcga
      601 tccgggaacg ggctgcggcc atgcgttcag cgaggcgttc gttctcggct tcagccgccg
      661 cgagttgctt gcggagtccg tcattttcgg tgcgcaggct gtggcacagc gcgaccacct
      721 gggagatctt gctctcgagt gtgttcagtt cattcagcat gggccagact atagattcaa
      781 catggccttc tggtcaaggt gtaagcgacg catagggatc aggtttgttg cgtgcacggc
      841 gcatttggtg gcgaacttca atcggcacgg tgtgggcgag ctataatgcg ttctttcgca
      901 gacctcgggc tgcggaagat cgtgcaaggt gccgctgtgc agcttcgctg ccgcggttaa
      961 acgggaaaca ggtgcgtgtg tgttcatcaa acaccatgcc tgtgctgccc ccgcaacggt
     1021 aagcgagtgt gggcgtgtcg aaatgccact gggcggaagc ctgggaaggc gacatgtcaa
     1081 gacttgcgag cccggatacc ggccttcgca cactactgga cggaagtgcc gcggggaagc
     1141 gggcacggaa cgctcgccgc ttgtgcgcct gttctccctc gcgcaagtcc gtatttatgg
     1201 ctcgccggct tgcggggacg ctgaccggca acagggagaa atccgtcatg tatccaaacc
     1261 catcgcgcgc cgctgccgcc acgacgttcg ctgcatccgt cgcggccttg tcctcgactg
     1321 cgccgcatgc cgccgatcag caactcgatt cggttgtggt cacggcgacc cgtcaggcga
     1381 cacgcagcaa tgagttgacc agtgatgtct cggtcatcac gcgcgccgaa atcgatcagg
     1441 ctggggaatc cacgctggcg caattgctgg cccgccaacc aggagtccag tacgttgcca
     1501 acggcggtgc aggcaccaac agcggtgttt tcatccgcgg cgccagtacc aatcaatcga
     1561 tcgtgttgat cgatgggcag cggatcggct ccgcaactac cggtagcgcg gcgctgtcgc
     1621 gcattccgct ggaccagatc gagcgcatcg aaatcctgcg cggcccggcg tcgtcactct
     1681 acgggcccga tgccatcggc ggggtgatcc agattttcac tcgtggcggc gaggggccga
     1741 cgcgagtgaa tgccagtagc ggttacggct cctacaatac gaccgatacc agcgtcggcg
     1801 ttgccggggg aacggatgtc gtttccttca gtgtgcaagc cggctatctc gacaccgacg
     1861 gcttcaatgc cagcaacaac cggcggaacg tgtcctacaa tcgcgaccgc gatggctact
     1921 acaacaagaa tctgagtgcc aacctttccg ttcgcccggc aagaggccag gagatcgggg
     1981 ttaatctact ggcgagcagc ggcaccaacc gctacgacgc caatgacttc agtgcgttgg
     2041 gggcggcgaa gaactacagc agcgaccaga acgtcggttc ctactcgatt tactcgcgca
     2101 atcgtctgca ttcggcttgg accagcactt tgcgccttgg tcgcagtacc gacgccagca
     2161 aggactatgt aggtaatctc agagacgcta ccttccgaac cgatatggac ctgctgtcgt
     2221 ggcagaatga cctccgcctt ccggttggcg agg
//
LOCUS       DAOSIT010000390         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41577_length_2249_cov_8.931176, whole genome shotgun sequence.
ACCESSION   DAOSIT010000390 DAOSIT010000000
VERSION     DAOSIT010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41577_length_2249_cov_8.931176"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLL14_09135"
     CDS             <1..267
                     /locus_tag="PLL14_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HOG04133.1"
                     /translation="IDAQAIHDMDVTAAQRFAELHRELAEEGIDVKIADAPRPFLEEL
                     AKVGLSDEIGRNDFFVSVKKAVEAFEQAQVSRSQAGLKYAASPR"
     gene            complement(329..1402)
                     /locus_tag="PLL14_09140"
     CDS             complement(329..1402)
                     /locus_tag="PLL14_09140"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOG04134.1"
                     /translation="MTTHDIDNINVSAFDPMPSPEEIHARLPLTASAASLVMHGREIL
                     RNILDRRDPRLFVVVGPCSIHDPVAGLDYAQRLKALADAVGETLYLVMRVYFEKPRTT
                     TGWKGYINDPDMDDSFRVDQGMHKAREFLLQLAELGMPTGTEALDPIAPQYLGDLMSW
                     TAIGARTTESQTHREISSGLSTPVGFKNGTNGDVGIAVNAILSASRPHSFLGINGQGR
                     TSIVRTRGNRYGHLVLRGGDGRPNYDTVSVQMAEQALRKAGLPSNIVVDCSHANSYKK
                     HDLQPLVMADAVNQVRLGNKSLVGMMIESNIVAGNQAIPDDLSQLKYGCSVTDACVDW
                     ETTEKMISDAATLLRTVLPGRFG"
     gene            complement(1416..>2249)
                     /locus_tag="PLL14_09145"
     CDS             complement(1416..>2249)
                     /locus_tag="PLL14_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG04135.1"
                     /translation="CTTIIETGIDNPHANTIVINRAEKFGLAQLHQLRGRVGRSHHQA
                     YAYLLTHDDAALNKQARQRLEAIQMMDELGAGFYLAMHDLEIRGAGEVLGANQSGEMQ
                     EVGFNLFTEMLNRAVAALKQGKEPDLTQPLAIATEIKLHTPALLPDDYAPDVHERLTL
                     YKRLADCQETEALDKLQEELVDRFGELPQQARALLDSHRLRLLCKPLGISKLDAAVEQ
                     VVVQFEPDPPIDAMRIINLIQKDRNYRLAGQDKLILRRHCATLAERVAAVKDIIHQLS
                     K"
BASE COUNT          391 a          628 c          747 g          483 t
ORIGIN      
        1 atcgatgcgc aggcgattca cgacatggat gtcaccgccg cgcagcgctt tgctgagttg
       61 catcgtgaac tcgccgagga gggtattgac gtcaagattg ccgatgcccc gcggccattc
      121 cttgaagaac tcgccaaggt cgggctctcg gacgaaatcg gcaggaacga tttctttgtc
      181 tcggtcaaga aagcggtcga ggccttcgag caggctcagg tctccaggag ccaggccgga
      241 ttgaagtacg cggcttctcc gcgctaagcg cctaggcgca agcgttcctg cggaccgggc
      301 gaagaccggt ccgcaggcag gttcagtgtc agccgaagcg tccgggcagg accgtccgca
      361 gcagggtggc ggcgtcgctg atcatctttt cggtggtttc ccagtcgaca caggcgtcag
      421 tcactgaaca gccatacttg agttgcgaga ggtcgtccgg aatcgcctgg ttgccggcca
      481 cgatatttga ctcaatcatc attccgacca gcgatttgtt cccgaggcgg acctgattga
      541 ccgcgtcggc catgaccagc ggttgcaggt catgtttctt gtaactgttg gcatgcgagc
      601 agtcgacgac gatgttgctc ggcaaacccg ctttgcgcag cgcctgttcg gccatctgca
      661 cgctgaccgt gtcatagttt ggtcggccgt cgccgccacg caggacgagg tgaccgtagc
      721 ggttgccgcg cgtgcgcacg atcgacgtgc ggccctggcc gttgattccc aggaagctgt
      781 gtgggcgcga ggccgagagg atggcgttga cggcgatacc gacatcgccg ttggtgccgt
      841 tcttgaagcc gaccggtgtg gacagtccag aagagatctc gcggtgcgtc tgcgactcgg
      901 ttgtgcgggc gccgattgcc gtccaggaca tcaaatcgcc gaggtattgc ggggcgatcg
      961 ggtcgagtgc ttcggtgcca gtgggcatgc caagttctgc gagttgcaac agaaactcgc
     1021 gtgccttgtg catgccctga tcgacgcgga aggagtcgtc catatccggg tcattgatat
     1081 aacctttcca accggtcgtc gtgcgcggct tttcgaaata cacgcgcatc accagataga
     1141 gcgtctcgcc gaccgcgtcg gcaagcgcct tgagacgctg cgcatagtcg agcccggcca
     1201 ccggatcatg gatcgagcac ggaccgacga cgacaaacag ccgcgggtcg cggcggtcga
     1261 gaatgttgcg caagatttct cgcccgtgca tcaccaggct ggcggcactt gccgtaagcg
     1321 gtaaacgggc atggatttcc tccggcgaag gcatggggtc gaaggcgcta acgttgatgt
     1381 tgtcgatgtc gtgggtagtc atggcgtccg caggttcatt tgctcagttg atgaataata
     1441 tcctttaccg cagcaacgcg ttcggcgagc gttgcgcagt ggcgtctgag gatcagcttg
     1501 tcctgtcctg ccagccggta gttgcggtct ttctgaatca ggttgatgat gcgcatggcg
     1561 tcgatcggcg ggtcaggctc gaactgcacg acgacctgct ccactgcggc gtcgagcttg
     1621 ctgattccga gcggcttgca gagcagccgc aggcggtggc tgtcgagcag tgcccgggcc
     1681 tgctgtggta actcgccgaa gcggtcgacc aattcttcct gcagcttgtc cagggcttcg
     1741 gtctcctggc agtcggccag gcgtttgtag agcgtcaacc gttcatggac gtccggtgca
     1801 tagtcgtcgg ggagcaaggc gggggtgtgc agtttgatct cggtggcgat ggccagtggc
     1861 tgcgtgaggt ccggctcctt gccttgtttg agcgccgcga cggcacgatt gagcatctcg
     1921 gtgaagagat tgaagccgac ttcctgcatt tcaccagact ggttggcgcc cagcacttcg
     1981 ccggcaccac ggatttcgag gtcatgcatg gcgaggtaga aaccggcacc gagttcgtcc
     2041 atcatctgga tggcctcgag gcgctgcctg gcctgtttgt tgagcgcagc atcgtcgtgt
     2101 gtcagcaggt aggcgtaggc ttggtggtga gagcgtccga cccggccgcg gagctgatgc
     2161 aactgcgcca gaccgaattt ctctgcacga ttgatgacga tggtgttggc gtgcgggttg
     2221 tcgatgccgg tttcgatgat cgtcgtgca
//
LOCUS       DAOSIT010000391         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41598_length_2248_cov_10.234838, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000391 DAOSIT010000000
VERSION     DAOSIT010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41598_length_2248_cov_10.234838"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLL14_09150"
     CDS             complement(<1..634)
                     /locus_tag="PLL14_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG04136.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILAKKYGGVA
                     KAYDQIDAAPEEKARGITINTAHVEYETAKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVYLNKCDMVDDAELLELVDMEVRELLSK
                     YDFPGDDVPIIQGSALKALEGDTGAIGEGSVMALADALDAYIPTPQRAIDK"
     gene            complement(656..>2248)
                     /gene="fusA"
                     /locus_tag="PLL14_09155"
     CDS             complement(656..>2248)
                     /gene="fusA"
                     /locus_tag="PLL14_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG04137.1"
                     /translation="PIVIPIGKEDYFEGVVDLIKMKAIYWDEASLGMKFDYREIPGEL
                     QAEADEWRSKMVEAAAESSEELMDRYLEQGELSEADVIRGLRDRTIACEIQPALCGTA
                     FKNKGVQRMLDAVIDLLPSPVDIPPVAGEDENGESATREASDAAKFSALAFKLMTDPY
                     VGQLTFIRVYSGILKSGDTIFNPTKGRRERIGRVLQMHANNREELKEVLAGDIAACVG
                     LKEVTTGETLCDPAAVITLEKMVFPEPVIHVAVEPKTKPDQEKMGIALGRLAQEDPSF
                     RVRTDEESGQTIISGMGELHLEIIVDRLKREFGVDANVGAPQVAYRECIKKSVEQEGK
                     FVKQSGGRGQYGHVWLKIEPNEPGKGYEFIDAIKGGTVPREYIPAVDKGLQDSVTSGV
                     LAGFPVVDIKFTLFEGSYHDVDSNENAFRMAAAIAFKEGMRRASPTLLEPMMAVEVET
                     PEDYMGNVMGDLSSRRGMIQGMEDLPGNVKAIKAEVPLAEMFGYSTTLRSLSQGRATY
                     SMEFKHYAEAPKNVAEAVINKK"
BASE COUNT          405 a          734 c          604 g          505 t
ORIGIN      
        1 gcttgtcgat cgcccgctgc ggcgtcggaa tgtacgcatc cagcgcgtcc gccagagcca
       61 tcaccgaccc ttcgcctatc gctccggtgt cgccttccag cgccttcagc gccgatcctt
      121 gaatgatcgg cacgtcatcg cccggaaaat cgtacttcga gagcagttca cgcacttcca
      181 tgtcgaccag ttcgagcagc tcggcgtcgt caaccatgtc gcacttgttc agatagacga
      241 cgatgtacgg cacgccaacc tgacgcgcca gcaggatgtg ctcgcgggtt tgcggcatcg
      301 gtccgtccgc cgccgagacc accaggatcg cgccgtccat ctgcgccgct ccggtaatca
      361 tgttcttgat gtagtccgcg tgacccgggc aatcgacgtg ggcgtagtga cgcttcgccg
      421 tctcgtactc cacgtgcgcg gtgttgatcg tgatcccgcg cgccttttcc tccggcgccg
      481 cgtcaatctg gtcgtaggcc ttggccacac caccatactt tttcgccaga atggtcgtga
      541 tcgccgccgt cagcgtggtc ttcccgtgat ccacgtgccc aatcgttccc acattgacgt
      601 gcggcttcgt ccgttcaaat tttcccttgg ccattaccgt atcctcaaaa cagagtcatt
      661 ttttgttgat gactgcctcg gcgacgtttt tcggcgcttc ggcgtagtgc ttgaattcca
      721 ttgaataggt tgcgcgcccc tggctcagcg aacgcaacgt cgtcgaatat ccgaacatct
      781 ccgccagcgg cacttcggcc ttgatcgcct tcacgttgcc gggcagatct tccataccct
      841 gaatcatgcc gcggcgactg gagaggtcgc ccatcacgtt gcccatgtaa tcctcggggg
      901 tttcgacctc cactgccatc atcggctcca gcagggttgg cgatgcacga cgcatccctt
      961 ccttgaaggc gatcgccgca gccatgcgga aagcgttttc gttcgagtca acgtcgtgat
     1021 acgagccctc gaagagagtg aacttgatat cgaccaccgg gaagcccgca agtactccac
     1081 tggttaccga atcctgcaag cccttatcta ccgcgggaat gtattcgcgc ggcacggtgc
     1141 cacccttgat cgcatcgatg aattcgtaac ctttgccggg ctcattgggt tcgatcttca
     1201 gccagacgtg accgtactgg ccgcgcccac ccgactgctt gacgaacttg ccttcctgtt
     1261 ccaccgactt cttgatgcac tcgcggtaag ccacctgcgg cgcgccgacg ttggcgtcca
     1321 cgccgaactc gcgcttcagg cggtcaacaa tgatctccag gtgcagttcg cccatcccgg
     1381 agatgatcgt ctgccccgat tcctcgtcgg tacgcacacg gaacgaggga tcctcctggg
     1441 ccagccggcc gagagcaata cccatctttt cctggtcagg cttggtcttc ggctctacag
     1501 ccacgtggat caccggctcc ggaaagacca ttttttccag cgtgatgacc gccgccggat
     1561 cgcaaagcgt ttctccggtg gtcacttcct taagtccgac acaggcggcg atatcccccg
     1621 ctagcacttc cttgagttct tcacggttgt tggcgtgcat ctgcaacacg cgcccaatgc
     1681 gctcgcggcg acctttggtc gggttgaaga tggtatcacc cgacttgagg atcccggaat
     1741 agacgcgaat gaaggtcagc tggccgacat aggggtcggt catcagcttg aaagcaaggg
     1801 cggaaaactt ggctgcatcc gaggcctcgc gagtcgcaga ctccccattc tcatcctcgc
     1861 cagcgaccgg gggaatatca accggcgacg gcagcaggtc gatgacagcg tcgagcatgc
     1921 gttgcacgcc cttgtttttg aaggcagtac cgcaaagcgc tggttgaatt tcacaggcga
     1981 tcgtgcggtc gcgcagcccc ctgatgacgt ccgcctcgct gagttctcct tgctccagat
     2041 agcggtccat caactcttcg gaggattccg ccgccgcttc gaccatcttg ctgcgccact
     2101 cgtcggcttc ggcctgcagt tcgcccggaa tctcgcgata gtcgaacttc attccaaggg
     2161 aggcttcatc ccaatagatg gccttcatct tgatcagatc cacgacgccc tcgaagtagt
     2221 cctccttgcc gatcggaatc acgatcgg
//
LOCUS       DAOSIT010000392         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41710_length_2244_cov_10.167200, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000392 DAOSIT010000000
VERSION     DAOSIT010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41710_length_2244_cov_10.167200"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLL14_09160"
     CDS             complement(<1..644)
                     /locus_tag="PLL14_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HOG04138.1"
                     /translation="MRRHYPLHVHISTLFLALILLVCGVLGGIGYQLSSDLLAASAAD
                     LTTRISREALLEMEHIIEPAEVATRLLSLQRVTTATSLRERLDSVEFLRAALDSSAAL
                     SSLYVGYDNGDFFLVGRVGQGSEAEASRVPVGASYVVQSIERAGAEASGRFIYLDASL
                     KILRTDDRPDYAAAYDPRQRPWFQQAQSASTQVKTPPYLFYSSRKVGTTLANRAG"
     gene            825..1334
                     /locus_tag="PLL14_09165"
     CDS             825..1334
                     /locus_tag="PLL14_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOG04139.1"
                     /translation="MPHTMSRSSTGLPAFESKIIAPGEIAPRLLVLPRPLVFTNGCFD
                     LLHRGHVTLLAEARALGASLVVALNADASVRRLGKGDDRPVNTLSDRLAVIAALESVT
                     LVTWFDEDTPLQRILDCRPEILVKGGDWPIERIVGNREVTNWGGRVLSLPFIHQTSTS
                     ALLDKIRRL"
     gene            complement(1383..2231)
                     /locus_tag="PLL14_09170"
     CDS             complement(1383..2231)
                     /locus_tag="PLL14_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG04140.1"
                     /translation="MNSQYNFSILEDIASDLSGEVVSFPIFLDITFQVRSALRKPDLT
                     IEQLGTVVSAEPLMSTKIIRLANSAAINRSGRPIVDINSAIARIGMEAVRTVSFAVAM
                     EQLLSSKKMAPFEALSRRLWEHTVHVAALSRVLARRLTRVHPDEAMFVGLVHDIGVFY
                     LLSRAANFPELVDDPAELHQLLVQWHDSIGHALLAAMGLPEEILVVVQEHEMERIVAS
                     PTTLAEVIFVANKLANVQHAWRDPTLCEPVDASILFELFDAEGLSTLLVESAEDVASL
                     KSALGT"
BASE COUNT          429 a          691 c          708 g          416 t
ORIGIN      
        1 ccggcacggt tggccagggt ggtgccgacc ttgcggctgg agtagaacag ataaggcggt
       61 gtcttgacct gtgtcgacgc tgactgcgcc tgctgaaacc agggccgctg ccggggatcg
      121 taggcggcag cgtagtccgg gcggtcgtcg gtgcgcagga tcttcagact ggcgtcgaga
      181 tagatgaagc ggccactggc ctcggcgccc gcccgctcga tgctctgaac gacgtagctg
      241 gcgccgaccg gtactcgcga tgcctcggct tcactgccct gcccgacacg gccaacgagg
      301 aagaagtcgc cattgtcgta gccgacatag agcgacgaca gcgccgcaga actgtctagc
      361 gccgcgcgca gaaattcgac gctgtcgaga cgttccctga gggaagtggc cgtggtcacc
      421 cgttgcaggc tcagcaggcg ggtggccacc tccgccggtt cgatgatgtg ttccatttcg
      481 agcagcgctt cgcggctgat gcgggtggtc aggtcggcgg cggacgcggc cagcaggtcg
      541 gagctgagct ggtagcctat gcctccgagg acgccacaga cgagcaggat cagcgccaga
      601 aacagcgtcg agatatggac gtgcaacggg taatggcgac gcatctgtcg gccctcggag
      661 tacgatagat ggatctaacg ataccgaaaa atgaactgcg cgtgcgcgct cacaattttc
      721 gccatcccgg agggaccatc tggcaatgac cgatcggatg cacaaacagc gcttggcccg
      781 cgtttcgaga accggtacga tgagcgctct tcgccgagtg aggcatgccc cataccatgt
      841 cccgatcatc gaccggcctg ccggccttcg aaagcaagat cattgccccc ggcgagatcg
      901 caccgcgcct gctcgtcctg ccacgcccac tggtgtttac caacggctgc ttcgaccttc
      961 tgcaccgcgg tcatgtcacc cttctcgccg aggcgcgtgc gctcggtgcg tcgctggtcg
     1021 tggcgctcaa cgccgatgcc tcggtcaggc gccttggcaa gggcgacgat cgtccggtaa
     1081 acaccctgtc tgatcgactg gcggtgatcg cagctctcga atcggtgacg ctggtcacct
     1141 ggttcgacga ggatacaccg ctgcaacgga tcctcgattg ccggccggag attctggtca
     1201 agggtggcga ctggccgatc gagcgaatcg tcggcaaccg tgaagtcacc aactgggggg
     1261 ggcgggtact gtccttgccg ttcattcacc agacctcgac cagtgcgctg ctcgacaaga
     1321 tccggcgcct gtagtcagcg tggttcggac gcggccagga tcaggcctgc cgcgccacgg
     1381 tttcaagttc ccagagcgct cttcagagag gcaacgtctt cggccgactc gacaagcagc
     1441 gtgctgagcc cttcggcgtc aaacaactca aacagaatgc tggcgtcgac cggctcgcag
     1501 agcgtgggat ctcgccacgc gtgctgtaca ttggccagct tgttggccac gaagatcacc
     1561 tcggcgagcg tcgtcgggct agctacgata cgctccatct catgctcctg caccaccacc
     1621 aggatctctt ccggcaaccc catggccgcc agcagtgcat gcccgatact gtcgtgccac
     1681 tgcaccagca actggtgcaa ctcggccgga tcgtcgacca gttcagggaa gttcgcagcc
     1741 cgcgagagca ggtaaaagac accgatatcg tggaccagtc cgacaaacat cgcttcgtca
     1801 ggatgtaccc gggtcagtcg gcgagccaat acgcgcgaca gagcggccac gtgcacggtg
     1861 tgctcccata gccgacgcga caacgcttcg aacggcgcca tcttcttcga actgagcagt
     1921 tgctccatgg cgacggcaaa ggacacggtg cgtacggcct ccatgccgat acgcgcaatc
     1981 gcactgttga tgtcgacgat gggccgcccg gagcggttga tcgccgccga gtttgccaga
     2041 cggatgatct tggtgctcat caggggttcg gcactgacta ccgtgcccag ttgttcgatg
     2101 gtcaggtcgg gtttccgcag cgcgctgcgc acctggaagg tgatgtcaag gaagatggga
     2161 aaggaaacca cctcgccgga aagatcgctg gcgatgtctt cgaggatgct gaagttgtat
     2221 tgactgttca ttctgtgctt atga
//
LOCUS       DAOSIT010000393         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41711_length_2244_cov_9.167200, whole genome shotgun sequence.
ACCESSION   DAOSIT010000393 DAOSIT010000000
VERSION     DAOSIT010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41711_length_2244_cov_9.167200"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLL14_09175"
     CDS             <1..513
                     /locus_tag="PLL14_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04141.1"
                     /translation="ERIAALVLEFEGSVVEVLTAATDARIDPDGKVTFSLAGIEGFHY
                     AARGGELSFGHGDASLAIVQPAAGSLEQRMEQRSARGPQPLTPPAGSNPAGEVWQLLK
                     EILQYPLVWLLLLLLLVGKVALLVARYRARRRTRRSGSGNRHARLKVRRTRLRHRIKR
                     VASSASLQEQ"
     gene            640..>2244
                     /gene="gpmI"
                     /locus_tag="PLL14_09180"
     CDS             640..>2244
                     /gene="gpmI"
                     /locus_tag="PLL14_09180"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG04142.1"
                     /translation="MLQKSASFPGIQGPIVTIVMDGYGIARSDVGSAIAAARKPTLDH
                     LFAKYPNITLRAHGTAVGMPSDEDMGNSEVGHNAIGAGQVYSQGAALVAGAIASGSIW
                     EGAAWRQVIAGTKAGRGVIHFIGLFSDGNVHSHIDHLQALVLRAKEEGVPVARIHILI
                     DGRDVPETSALDYLLPFEAFLADVSGGGFDARVASGGGRMNITMDRYEANWGMVEKGW
                     RTHVLGEGEQYDRASAAVEALRLKFPGTIDQDLPPFVIASGGRPVGTIEDGDSVVFFN
                     FRGDRSIEITRAFEEATFDKFDRVRVPNVTYAGMLQYDGDLKLPKRFLVDPPAILDTM
                     GEWFAKSGITQFACSETQKFGHVTYFWNGNRSNKFAGETYQEVLSDVVPFEQRPWMKA
                     AEIADAMIEALKSGQYRTLRCNFANGDMVGHTGSFRAATMAIEAVDLQLARILPVINA
                     LGGVALITADHGNADEMYEIDKKTRQPAQNPDGSFKAKTSHTLNPVPLILYDNVTGGK
                     LALRQDPGAGLSNIAATIANLLGLEKH"
BASE COUNT          437 a          700 c          686 g          421 t
ORIGIN      
        1 gagagaatcg ccgcgctggt cctcgaattc gaggggtcgg ttgttgaggt actcaccgca
       61 gccacggacg cacgcatcga tccggatggg aaggtcacct tctcattggc cggcatcgaa
      121 ggtttccatt acgctgcgcg aggcggggaa ttgtcgtttg gacacggcga tgcttcgctg
      181 gccatcgtcc agccggctgc cggctcgctt gagcagcgca tggaacaacg cagtgcccgt
      241 ggcccacaac ccttgacccc gccagcaggc tcgaaccctg ccggggaagt ctggcaactg
      301 ctcaaggaaa ttctgcagta tcccctggtc tggctgttgc tcctgctgct gttggtcggc
      361 aaggtcgccc tgctggtcgc gcgctaccgg gcccggcggc gaacgcgcag aagcggttcc
      421 gggaaccggc acgccaggct caaggtcagg cgtacgcgct tgcggcaccg gatcaagcgg
      481 gtagcctcat cagccagcct ccaggagcag taatcgccgt agttggccca gacaaccgcg
      541 gtcgccagaa ggggcttgcg gtatactggc acagacgccg ctgcttgccg atgcgtagtg
      601 gcctttgctc ccaactctcc acctccccag gaatccatca tgttgcagaa gtctgcctct
      661 ttccctggaa ttcagggccc catcgtcacg atcgtcatgg atggctacgg cattgccagg
      721 tccgatgtcg gcagtgccat cgctgctgcc aggaaaccca ccctcgacca tctctttgcc
      781 aagtacccga atatcacctt gcgcgcccac ggtaccgccg tgggcatgcc ttccgacgaa
      841 gacatgggca actccgaagt cggtcacaat gccattggcg ccgggcaggt ttacagccag
      901 ggcgcagccc tggtcgccgg agcgatagcc tccgggtcga tctgggaggg cgcggcctgg
      961 cggcaggtca ttgccgggac caaagccggc cgcggggtga tccacttcat cggcctcttt
     1021 tccgatggca atgtgcacag ccacatcgat catctgcaag ccctggtcct gcgcgccaag
     1081 gaagaaggtg taccggtggc gcggattcac atcctgatcg acgggcgtga cgtcccggaa
     1141 accagcgccc tcgactatct gctgcctttc gaggccttcc tggccgacgt gagcggcggc
     1201 ggattcgacg cccgtgtcgc ctcgggtggt ggccggatga acatcaccat ggatcgctat
     1261 gaggcgaact ggggcatggt cgaaaagggc tggcgcacgc atgtcctcgg cgagggcgaa
     1321 cagtacgacc gtgcttcggc tgccgtagag gcattgcgtc tcaagtttcc aggaacgatt
     1381 gaccaggatc tgcctccatt cgtgatcgcc agcggcggcc ggcccgttgg taccatcgag
     1441 gacggtgact cggtagtctt tttcaacttt cgcggcgacc gctcgatcga gatcacgcgc
     1501 gcctttgaag aggccacttt cgacaagttc gaccgcgtgc gcgtgcccaa cgtgacctac
     1561 gccgggatgt tgcagtatga cggcgacctc aagctcccga aacgctttct ggtcgatcca
     1621 ccagccattc tcgacaccat gggcgagtgg tttgccaaat caggcattac ccagtttgcc
     1681 tgctcggaga cgcagaaatt cgggcatgtg acctatttct ggaatggcaa ccgctcaaac
     1741 aagttcgccg gcgagaccta tcaggaagta ttgagcgacg tggtgccctt cgagcaacgg
     1801 ccgtggatga aggcagcgga aatcgccgat gccatgatcg aagccctgaa gagtggccag
     1861 taccggacct tgcgctgcaa tttcgccaat ggcgacatgg tcgggcacac tggcagtttc
     1921 cgcgcggcga cgatggccat cgaggcggtc gatctgcaat tggcacgcat cctgccggtg
     1981 atcaacgcac tcggcggcgt ggcgctgatc accgctgatc acggcaatgc cgacgagatg
     2041 tacgaaatcg acaagaaaac caggcaacca gcgcagaacc cggatggatc gttcaaggcc
     2101 aagacatcgc atacgctgaa cccggtgcct ctgatcctct acgataacgt gaccggcgga
     2161 aaactggctc tcagacagga tcccggcgcc gggctttcca atatcgcggc gacgatcgcc
     2221 aatcttctcg gcctggagaa gcac
//
LOCUS       DAOSIT010000394         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41822_length_2240_cov_9.281465, whole genome shotgun sequence.
ACCESSION   DAOSIT010000394 DAOSIT010000000
VERSION     DAOSIT010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41822_length_2240_cov_9.281465"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PLL14_09185"
     CDS             complement(<1..1066)
                     /locus_tag="PLL14_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG04143.1"
                     /translation="MDVFQFREKLVGEYSQFTRSFTRIRTADIASFVAAAYESQRYWP
                     APLVQVNPNFKAEHTVEELVAASGLHPECARIFRGGKSVTSAGVSLKLFRHQEEAISF
                     AQTGASYVLTTGTGSGKSLAYFIPIIDAILKARAADPAPRTRAIVIYPMNALANSQIE
                     ELGKFLGEYGDQPPVTFGRYTGQEDDEERQRLATHPPDILLTNFMMLELLMTRQDDKD
                     KAVMRNAKGLCFLVLDELHTYRGRQGADVALLVRRVREALAGRLQCIGTSATMATGGS
                     EADRNAVVAEVATRLFGTTVSPAHVITETLQRITPENLLVEHIRASLATAIRAGLPEN
                     PAFSDLAQHPLAVWTELTLGL"
     gene            1322..>2240
                     /locus_tag="PLL14_09190"
     CDS             1322..>2240
                     /locus_tag="PLL14_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOG04144.1"
                     /translation="MSSMERIYQIDQILGARPFATRADLLERLGVSWATLKRDIAYMR
                     DRLNAPVVFDRSLGGYRFEKPEQQVGPQYELPGLWFTAEEIHALLTMQHLLSNLDTGG
                     LLGPQIQPLLARLSGLLGAAENPVEEVRRRIRIQTVGAREFHLDHFQAVGSALLRRKR
                     LVIRYHARGTDKLTEREVSPQRLNYYRDNWYLDAWCHLRNGLRAFSVDAIERVEILDK
                     RAKDIADTRLDEVLGSGYGIFAGDQVSWAVLRFTPERARWVASERWHPKQQGQFLDDG
                     SYELRVPYADNRELIMDIMKYGSDCQVIEP"
BASE COUNT          397 a          709 c          692 g          442 t
ORIGIN      
        1 ccaggccgag ggtgagttcc gtccatacgg ccagcgggtg ctgcgccagg tctgagaatg
       61 caggattctc gggcaggccg gcccggattg ccgtcgccag ggaggcgcgg atgtgttcaa
      121 ccaggaggtt ttccggggta atgcgttgca gtgtttcggt gatcacatgc gcgggcgaga
      181 ccgtggttcc gaatagccgc gtggcgactt cggcaacgac tgcattgcga tcggcctcgc
      241 tccctcccgt tgccattgtg gccgaggtgc cgatacactg cagccggccg gccaacgcct
      301 cacgaacccg tcgcaccagc agcgccacgt ctgcaccctg gcgtccgcgg taggtgtgca
      361 actcgtcgag caccagaaag cacaagccct tggcgttgcg catgacggcc ttgtccttgt
      421 cgtcctgacg ggtcatcagg agttcgagca tcatgaagtt ggtgagcagg atgtccggag
      481 gatgcgttgc caggcgctgg cgttcttcat cgtcctcctg gccggtgtaa cggccgaagg
      541 ttaccggtgg ctggtccccg tattcgccca ggaacttgcc gagttcctcg atctggctat
      601 tggccagcgc gttcatcggg tagatgacga tggcgcgagt ccgcggcgcg gggtcggcgg
      661 cccttgcctt gaggatggca tcaatgatcg ggataaagta ggcgagcgat ttgcccgagc
      721 cggtaccggt ggtcaggacg tagctggcac ccgtctgagc gaagctgatc gcttcttcct
      781 ggtgcctgaa gagtttcagc gacacgccgg ccgaagtgac actcttgccc ccccggaaaa
      841 tgcgcgcgca ctccgggtga agaccgctgg cggcgaccag ttcctcaacg gtgtgctcag
      901 ccttgaagtt gggattgacc tggacgagcg gtgcaggcca gtagcgttgc gactcgtagg
      961 cggcggcgac aaaggacgcg atgtcggcgg tcctgatgcg cgtgaaactg cgggtgaact
     1021 ggctgtattc accgaccagt ttctccctga attggaaaac gtccatgctc gccctgcctt
     1081 ttttattttc ctgctcagcc gcacctgcct cactgcgcag gacagccttt ggcgcatgca
     1141 ccacagcctg gcaacatgcc cctcttgaat gctcgaccgg cacgctcccc aggatcaccg
     1201 cctctcttcc tcacaaccct ggcatcaact ctgccaatga gcgtaattgc gtgccatgat
     1261 acagccttgg aagctcatct ttcgagcccg caactgaatc tttcgtcgtc ggattttatc
     1321 tatgagcagc atggaacgta tctaccagat cgaccagatt ctcggcgccc gcccattcgc
     1381 gacgcgcgcg gatctgctgg agcgcctggg tgtctcctgg gcgacgctga agcgcgacat
     1441 cgcctatatg cgcgatcgct tgaatgcccc ggtcgtcttc gaccgcagtc tgggcggcta
     1501 ccgcttcgag aagcccgaac aacaggttgg cccgcagtat gaactgccgg gcctgtggtt
     1561 caccgccgag gagatccatg cgctgctgac catgcagcat ctgctctcga acctcgatac
     1621 cgggggactg cttgggccgc agatccagcc cctgctggcc cgtctctcgg gcctgctcgg
     1681 cgctgccgag aacccggtcg aggaagtccg gcgccgcatc cgtatccaga cggttggcgc
     1741 ccgcgaattt catctcgacc acttccaggc cgtcggctct gccctgttgc ggagaaaacg
     1801 cctggtcatc cgctaccacg cgcgcggcac cgacaagctg accgaacggg aagtctcgcc
     1861 gcaacgcctc aattactatc gcgacaactg gtatctcgat gcctggtgtc acctgcgcaa
     1921 cggcctgcgc gccttttcgg tcgacgccat cgaacgcgtc gaaatcctcg acaagcgggc
     1981 caaggacatc gccgacacgc ggctggacga agtgctcggg tccggctacg gcattttcgc
     2041 gggcgatcag gtgtcgtggg cggtgttgcg cttcacgccg gaaagggcgc gctgggtcgc
     2101 ctccgaacgc tggcatccga agcagcaagg ccagttcctc gacgatggca gctacgaatt
     2161 gcgcgtgccc tacgccgaca accgcgaact gatcatggac atcatgaagt acggtagcga
     2221 ctgccaggtc atcgaaccgg
//
LOCUS       DAOSIT010000395         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41847_length_2239_cov_5.944139, whole genome shotgun sequence.
ACCESSION   DAOSIT010000395 DAOSIT010000000
VERSION     DAOSIT010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41847_length_2239_cov_5.944139"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..2040)
                     /locus_tag="PLL14_09195"
     CDS             complement(226..2040)
                     /locus_tag="PLL14_09195"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG04145.1"
                     /translation="MSGLRFKKWPIIMKIMAISAISIAFISGLNLFYFVPLIESRVVD
                     ARQNEIRNLVSMAHDIFDEYEVDVRKSQVTREDAQTRAAVRIKQLRFGENNYFWINDS
                     NYRMVMHPTRPDLDGLDQSGFKDPTGKFIFKEFVDASRRDGSGFIEYLWPKPGTTLPV
                     KKIAFVKLYEPWGWVIGGGVYVDDIEKDLRQLRIILMTGTAIFAVVTLSVAWIIGINI
                     TRPLHEVMQGLEEVAEGQTGVTLAKRIEITATDEIGLLTSKFNELMESLHRLSVFKKV
                     IEEDASLEQVYMRLHWVFTETIGIADCVIYEISASGGGMRQAYPVTIHDEELFCNPEI
                     LSDCELCKAKRTGHIIDSTTFPSVCTMFQNPDGSRIHYCFPIIVGGGTTGVILFRAPR
                     PASLEQARQTRARIGKAEHYINESLSVIETKRLMGNLRESSLRDPLTGLHNRRYLQEH
                     MDNLVASMTRRKKSIGVIMCDIDYFKQVNDVHGHHAGDLVLKETATRIGKSIRETDVV
                     IRFGGEEFLVILLDINGGESLEVAEKIRRNIEGTRFKVSDGTLEKTISLGISEFPDDS
                     GTLWECIKFADVALYRAKDQGRNQSVRFIREMWSESQV"
BASE COUNT          426 a          650 c          623 g          540 t
ORIGIN      
        1 aatacatcga gcgctgtgca gcctacctcg ccaccggtcg ggttcccgtc acgccggcgc
       61 cactcccggc ctgaaacgtg gtttgcaagg ggttatttgc aaagcgtttc gtctgcgatg
      121 tgaaagcttt gttcaggcct ccacggtacc ggtacttgcg ggtcgccccc atgcttgtcg
      181 gtgtcgttct ccccgacgga atggggctgc caacgcgagg tcttgctaaa cctgactttc
      241 cgaccacatc tcccggatga aacggacact ctggttgcgg ccctggtcct tggccctgta
      301 cagggccacg tcggcaaact tgatacactc ccagagggtg ccgctgtcgt cggggaattc
      361 actgatgccg aggctgatgg tcttttccag cgttccgtca gacaccttga agcgggtgcc
      421 ttcgatattc cggcgaatct tttcggcaac ttccaggctt tccccaccgt tgatgtccag
      481 caggatgacc agaaactctt cgccaccaaa gcggatcacc acatccgttt cacggatgct
      541 ttttccgatc ctggtcgccg tttccttgag aaccagatct ccagcatgat ggccgtgaac
      601 gtcgttgacc tgcttgaagt agtcgatgtc gcacatgatg acgccgatcg atttcttgcg
      661 gcgggtcatg ctggcaacga gattgtccat gtgctcctgc aggtagcgtc ggttgtgcag
      721 acccgtcaag ggatcgcgca gcgacgattc acggagattg cccatcaacc gcttggtctc
      781 aatgacggac agcgactcgt tgatgtaatg ctcggccttg ccgattctgg cccgcgtctg
      841 ccgggcctgt tcgaggctgg ctggccgggg cgcgcggaaa agaatgactc cggtggtgcc
      901 gccgccgacg atgatcggga agcagtagtg aattctgctg ccatccggat tctggaacat
      961 ggtacagacc gacggaaatg tcgtcgagtc gatgatatgg ccggtcctct tggccttgca
     1021 cagttcgcag tcactgagga tttccgggtt gcagaacagt tcttcgtcat ggatcgtgac
     1081 cgggtaggct tgcctcatgc cgccaccact ggctgatatt tcgtagatca cgcagtcggc
     1141 aatgccgatg gtttccgtga atacccaatg caggcgcata tagacctgtt caagagaggc
     1201 gtcctcctcg atgaccttct tgaatacgga caaacggtgc aaggattcca tcaactcgtt
     1261 gaacttcgaa gtgagcaagc cgatttcgtc agtagcggtg atttcgatcc gcttggcgag
     1321 tgtgacgccg gtttgccctt cggcgacttc ttccagccct tgcatgactt cgtgcagcgg
     1381 gcgggtgata ttgattccga tgatccacgc cacgctcagc gtgacaacgg caaaaatcgc
     1441 cgtgccagtc atcaggatga tccgcagttg ccgcaaatcc ttctcgatgt cgtcaacgta
     1501 taccccgccg ccgatcaccc atccccacgg ctcgtacagc ttcacgaagg cgattttctt
     1561 taccggcagg gtggttccag gcttgggcca caggtactcg atgaagccgc tgccatcccg
     1621 cctgctggca tccacgaatt ccttgaagat gaacttgccc gttgggtcct tgaagccact
     1681 ttggtcgagc ccgtcaaggt cgggcctggt gggatgcatc accatccggt agttgctgtc
     1741 gttgatccag aaatagttgt tttccccaaa gcgaagctgc ttgatgcgca cggctgcacg
     1801 cgtctgcgca tcctcccggg tgacctggct ttttctcacg tcgacttcgt actcgtcgaa
     1861 aatgtcgtga gccatcgaca cgaggttgcg gatttcattc tgtcgcgcgt ccaccaccct
     1921 gctctcgatg agcggcacga agtaaaagag attcaagcca ctgatgaagg caatgctgat
     1981 tgccgagatg gccatgatct tcatgatgat cggccacttc ttgaatctca gaccgctcac
     2041 ggctgctctc cgggcgtcag cagtgttcat ggacaagttc agcgagtccc caaaggcata
     2101 tgcctgctcg gctttggccg tcgaacatgc tatttgctgg cctcctgcag gtctggggat
     2161 cgacttgcca ttgactctcg gtgtgctctt gaggataacc agctcatgcc atgcccctga
     2221 agttgtggct agtgtagtc
//
LOCUS       DAOSIT010000396         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_41893_length_2237_cov_11.796517, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000396 DAOSIT010000000
VERSION     DAOSIT010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41893_length_2237_cov_11.796517"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="clpX"
                     /locus_tag="PLL14_09200"
     CDS             complement(<1..947)
                     /gene="clpX"
                     /locus_tag="PLL14_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG04146.1"
                     /translation="MLARETTPSAIVRYLDQYVIGQTEAKKILAVAVYSHYRKIATFQ
                     NDKGTIAKSNVLLVGPSGTGKTLLCDTLSRMLKVPFVTADATSLAQTKYVNEEIEAVL
                     QRLLEKADGNIENAQHGIIFIDEIDKLKATNAQSRAISGESVQHALLKIMEGAPVKLK
                     NDQYIDTSNILFICGGAFVGLENIMSKTHGFGFIATSADDNQNILDRLNKRVKPTDLF
                     EFGLIPEFTGRLPVVANLHPLTKAMLVSIMSVPRNSIYRQYIEIFRGEGVELSIGQRV
                     FEQIAEIAIEYKTGARSLRGIFEELITPILYVVPDNPEIS"
     gene            complement(1119..>2237)
                     /locus_tag="PLL14_09205"
     CDS             complement(1119..>2237)
                     /locus_tag="PLL14_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOG04147.1"
                     /translation="DLYLPSLPTLARVFASDVASVQLTLSVFLAGFACGQIVYGPLSD
                     RYGRRPVLLGGLLLFCVGSLGCVFAHSIDRLILARFVQALGACAGPVIGRAVVRDLWG
                     ASESARVIAYMGGAMAIGPLLGPTLGGFLTVLFGWQSNFVLLLLVAVVQLAVVARVLG
                     ESNVHRDPEATTPARMFANFRRLLADRSYLGYLLTFSFSYSALFAFISASSFVLTGRH
                     GLTPQVYGMCFGIVVAGYLIGSVASGRLVRRLGSDGLLARGACLGAVAGVSMAVLEFS
                     GVHGVAAILAPMFFCTVATGLVMPNAIARALAPYPTMAGSASALMGFVQMTMAAGVGI
                     AVGHSLAAGAQALAVAVALCSLLALLSYLALIRVRRAP"
BASE COUNT          473 a          680 c          707 g          377 t
ORIGIN      
        1 gagatctcgg ggttgtctgg tacgacgtag agaatcggcg tgatcagttc ctcgaagatg
       61 ccgcgcaggc tgcgcgcgcc ggtcttgtac tcgatggcga tttcggcaat ctgctcgaat
      121 acccgttggc cgatactcag ttccacgcct tcgccccgga agatctcgat gtactgccga
      181 tagatcgagt ttctcggcac gctcatgatg ctgaccagca tcgccttggt caaggggtgc
      241 aggttggcga ccaccggcag gcgtccggtg aattcgggaa tcaatccgaa ctcgaacagg
      301 tccgtgggct tgacgcgctt gttcagccga tcgaggatgt tctggttgtc gtcggccgag
      361 gtggcgataa aaccgaaacc atgcgtcttg gacatgatgt tttcgagacc gacgaaagcg
      421 ccgccacaga tgaacaggat attgctggta tcgatgtatt gatcgttttt cagcttgacc
      481 ggcgcaccct ccatgatctt cagcagcgcg tgttgcacgc tctcgcccga gatggctcgc
      541 gactgcgcat tggtcgcctt cagtttgtct atctcgtcga tgaagatgat gccatgctgt
      601 gcattctcga tgttgccgtc ggctttttcg agcagccgct gcaataccgc ctcgatctct
      661 tcattgacgt acttggtctg cgccagagac gttgcgtcgg cggtcacgaa aggtaccttg
      721 agcatccgcg acagggtgtc gcagagcagc gtcttgccgg tcccggaagg cccgacgagc
      781 aggacgttgc tcttggcgat cgtccccttg tcgttctgga aagtggcgat cttccgataa
      841 tgcgagtaga ccgcgacggc caggatcttt tttgcttcgg tctggccaat cacgtattga
      901 tcgagatacc tgacgatggc gctgggcgtg gtttctctgg caagcaattc gcacctcaag
      961 atcggcaata ggagttcaca agactttcgg aaggcgctcg tgctggcctg cgggttgccc
     1021 cccaaatgat gatcttatgc cgaaaccgaa aaacgtgccg tgcgtggcga actccaggat
     1081 gggcggaggg aaatccgcca ggatcagaca gccggccctc agggagcccg gcgcacccgg
     1141 atcagcgcca ggtacgacaa cagggcgagg agcgagcaca gcgccacggc caccgccagg
     1201 gcctgcgcac cggcggcaag gctgtgaccg acggcaatgc cgacgcctgc ggccatcgtc
     1261 atctgtacga agcccatcag ggccgaagcc gatccggcca ttgtcggata cggtgccagc
     1321 gccctggcaa tggcattcgg catcaccagt ccggtcgcga cggtgcagaa aaacatcggc
     1381 gcgaggatgg cggcgacgcc atgcacgcct gaaaactcca gcaccgccat gctcaccccg
     1441 gcgaccgcac ccaggcaggc gccacgtgcc agcaggccat cgctgccaag ccgccggacc
     1501 agtcggcctg aagccaccga gccgatcaga tagccggcga cgacaatgcc gaaacacatg
     1561 ccgtagacct gcggggtcag tccgtggcgg ccggtcagga cgaatgacga agccgagatg
     1621 aaggcgaaca gggcgctgta cgagaacgag aaggtcagca gatagccgag gtagctgcgg
     1681 tcggcaagca ggcgccgaaa gttggcgaac atccgcgctg gcgtggtcgc ttccggatcg
     1741 cgatgcacat tgctttcgcc gagcacgcgg gcgacgacgg cgagttgcac cacagccacc
     1801 agcaacagga gcacgaaatt ggattgccag ccgaagagta ccgtcaggaa accgccaagc
     1861 gtcggcccga gcagggggcc gatggccatc gcgccaccca tgtaggcaat gacgcgtgcc
     1921 gactcgctgg caccccagag gtcgcggacg acggcccggc cgatgaccgg acccgcgcag
     1981 gcaccgagtg cctgcacgaa gcgcgcgaga atcagccggt cgatgctgtg ggcaaacacg
     2041 cagcccagcg agccgacgca aaacagcaac aggccgccga gcaggaccgg ccgccgcccg
     2101 tagcgatcag agagcgggcc gtaaacgatc tgcccgcagg caaagccggc aagaaacacc
     2161 gacagggtca gttgtacgct ggccacatcg ctggcaaaca ccctggcgag cgtcggcaac
     2221 gaaggcagat aaaggtc
//
LOCUS       DAOSIT010000397         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42025_length_2232_cov_10.177768, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000397 DAOSIT010000000
VERSION     DAOSIT010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42025_length_2232_cov_10.177768"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLL14_09210"
     CDS             <1..990
                     /locus_tag="PLL14_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04148.1"
                     /translation="AWAMAGLTLFAYTFLMFWNLPLPPPQGDLAQFDALLARATGGTE
                     HAAHGSGFALHVLGMWLNFVISLGVVAFFLTRMATTLKNRERELAATREAALRNEQIL
                     SLGTLAAGAAHQLGTPLATMAVVIRELELQHGDDAELREDLLLLREQVDRCKQTISQI
                     LASTGQGRDESLCSLALDAYLQRLLDDWQVIRPHARISVTLQGEQPPPLVTAERTLEQ
                     ALLNLLDNAADAHAANPEALRFSASWDSDSCRIEIFDRGPGIGGEAALRLGQAFFSSK
                     GAAHDQAGGIGIGLFLSNATIERCGGKVELFNRSDPQGGACTRVTLPLNRLKA"
     gene            996..1562
                     /locus_tag="PLL14_09215"
     CDS             996..1562
                     /locus_tag="PLL14_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG04149.1"
                     /translation="MPGRSPQAADDERSTLLLVDDDEAFRRVLARALDRRGYAVSVAA
                     TVAAAIVKAQAQSPEYAVVDLKMPGQSGLVLIEKLMELDPNTRIVMLTGYASIATAIE
                     AIKLGAIHYLAKPCDADEIVAALNKSGGGDSAIPVTGSPLSIDRLEWEHIQRVLVEHQ
                     GNVSATARALKMHRRTLQRKLGKHPSRE"
     gene            1565..1969
                     /locus_tag="PLL14_09220"
     CDS             1565..1969
                     /locus_tag="PLL14_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG04150.1"
                     /translation="MSSTAQAALERMRIDKWLWAARFYKTRSLAAQAVATGHVRLDGH
                     SLKPARELRCGDQLAITIGDQTWTITVRGLSDQRRPAAEAQQLYEEDSESCARRAAQR
                     EARQLAPVPGSDLRGRPTKKARRQIKGFNDSF"
     gene            2034..>2232
                     /locus_tag="PLL14_09225"
     CDS             2034..>2232
                     /locus_tag="PLL14_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOG04151.1"
                     /translation="MTHRVLADGIVVLHLLFIGFVVVGGVFVLRWPGLALVHLPAACW
                     GAAIELSGGICPLTPLENQFRQ"
BASE COUNT          417 a          766 c          698 g          351 t
ORIGIN      
        1 gcctgggcga tggcgggact caccctcttc gcctacactt tcctgatgtt ctggaacctg
       61 ccactaccac caccgcaggg tgacctggcg cagttcgacg ccctgcttgc ccgcgccacc
      121 ggcggcaccg agcacgccgc gcacggcagt ggcttcgcgc tgcacgttct ggggatgtgg
      181 ctcaacttcg tgatcagcct cggcgttgtg gccttcttcc tgacgcgcat ggcgacgacg
      241 ctcaagaacc gtgagcgcga actcgccgcg acccgtgaag cggcactgcg caacgaacag
      301 attctctcgc tcggaacgct ggctgccggt gcggcgcacc agttgggcac gccactggcg
      361 acgatggccg tggtgatccg tgaactcgaa ctgcaacacg gcgatgacgc cgaattgcgg
      421 gaagatctgc tgttgttgcg tgaacaggtc gaccgctgca agcaaacgat ctcgcagatc
      481 ctcgcgtcga ctggccaggg ccgtgacgaa agcctgtgct cgctggcact cgacgcctac
      541 ctgcagcgtc tgctcgacga ctggcaggtg atccgcccgc atgcccggat cagcgttacg
      601 ctgcaaggcg agcaaccccc cccgctggtc accgccgagc gcacgctcga acaggcgctc
      661 ctcaatctgc tcgacaatgc cgccgatgcg cacgcggcga acccggaagc gctccgcttt
      721 tcggcgtcct gggacagcga cagctgtcgc atcgagattt tcgatcgcgg cccagggatc
      781 ggcggagaag cggcgctgcg tcttggccag gcgtttttca gcagcaaggg cgcggcgcac
      841 gaccaggccg gcggcatcgg catcggactg tttctcagca acgccaccat cgagcgctgc
      901 ggcggcaagg ttgaactgtt caatcgcagc gacccgcagg gcggcgcctg cacccgcgtc
      961 accctccccc tgaaccgcct gaaagcgtga tcaagatgcc tggacgaagc ccgcaagccg
     1021 ccgatgacga acgctcgacg ctgctcctgg tcgacgacga cgaagctttc cgacgcgttc
     1081 tcgcacgtgc cctggatcga cgcggttatg ccgtgagtgt cgccgccacg gtcgcggcag
     1141 ccatcgtcaa ggcacaggca cagtcgccgg agtacgccgt cgtcgacctc aagatgcccg
     1201 gccagtcggg actggtattg atcgagaaac tgatggaact cgacccgaac acgcgcatcg
     1261 tgatgctcac cggctacgcc agcatcgcca ccgccatcga ggcgatcaaa ctcggcgcca
     1321 tccactacct ggcgaaaccg tgcgacgccg acgaaatcgt cgcggcgctc aacaagagcg
     1381 gcggcggcga ctcggcgatc ccggttacgg gctcgcccct gtcgatcgat cggctcgaat
     1441 gggaacacat ccagcgtgtc ctggtcgagc atcagggcaa tgtctcggca accgcaagag
     1501 ccctcaaaat gcatcgccgc acgctgcagc gcaagctcgg caaacacccg agccgggagt
     1561 aaccatgagc agcaccgcac aagcggcact ggagcgcatg cgtatcgata aatggctgtg
     1621 ggcggcgcgc ttctacaaga cgcgctcact ggccgcacag gccgtcgcca ccggccacgt
     1681 ccggctcgac gggcactcgc tcaaaccggc gcgcgagttg cgctgtggcg accaactcgc
     1741 aatcaccatc ggcgaccaga catggacgat caccgtccgc ggtctctccg atcaacgccg
     1801 cccggcggcc gaggcacagc aactctacga agaggacagc gagagctgcg cgcggcgagc
     1861 ggcgcagcgg gaggcgcgac agctcgcgcc ggtacccggt agtgacctgc gtggtcgacc
     1921 caccaagaag gccagacgcc agatcaaggg tttcaacgac agtttctgaa ccggggggcg
     1981 gcgacgatga cccagaccat gctcggcgaa ctttcgaatc ccggtccgca agcatgaccc
     2041 atcgagtcct cgcagacggc atcgtcgtcc tgcacctgct gttcatcggc ttcgtcgttg
     2101 tcggtggagt cttcgtactg cgctggccag ggctggcgct ggtgcacctc ccggcggcct
     2161 gctggggcgc ggcaatcgaa ctgagcggcg gcatctgtcc cttgacgccg ctcgagaatc
     2221 aattccggca gg
//
LOCUS       DAOSIT010000398         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42118_length_2228_cov_9.230557, whole genome shotgun sequence.
ACCESSION   DAOSIT010000398 DAOSIT010000000
VERSION     DAOSIT010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42118_length_2228_cov_9.230557"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>2228
                     /locus_tag="PLL14_09230"
     CDS             76..>2228
                     /locus_tag="PLL14_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG04152.1"
                     /translation="MLDACRHPTRRGVIRALLAVPALLLLYVLILIPFTPSISNIRKA
                     SSEQPAQVLSADGRKLAEFKQSNRQWVALSEISPQVINALIATEDHRFYDHFGLDWRR
                     TAAAALNTFRGDRQGGSTLTQQLARNLYPEAIGRDQTLTRKIKEAITALKIEAIHSKD
                     EILETYLNTVPFLYNAYGIEMAARTYFDTSADQLDVLQSATLVGMLKGNSYYNPVLNP
                     ERSLQRRNTVLKQMVRREKLTQAQFDALSRKPLRIDFERQTEPPGPAPHFARHLRKWL
                     IAWADRNGYSIYGDGLVVRTTIDSRLQAAANQAVARQGNSLQNIANAAWGKRSAWSTD
                     SSLVQAFIRESPEYAAAIAKGRTPEQARQELSADKAFMRALQRQKTQIQIGFLAIDPR
                     NGHVKAWVGSRDFEQDAFDHVEQARRQPGSTFKPFVYGEAFRQGASPDDRLIDQAVEI
                     HVGGAEIWRPTDGTPPSGEAMRLSDGLAYSKNTITAQLIQQVGPAKVAKLARHMGVRQ
                     SKLEAVPSLALGTSPVTLKEMVAAYGTIANGGGYLEPLLVSRIEDRDGRVIEEFSPPV
                     PDVALERDVAYTLLDSLRGVINRGTGKDIRGRYGIRADVAGKTGTTQDNADGWFILMH
                     RQLVAGAWVGFNDSRVTLRSDYWGQGAHSALPVVGDFFGNALRTRLIDPRARFAAPEE
                     TGLWARIKNWFRSVFSQEPAAEARPQRIVKPTRKQ"
BASE COUNT          442 a          765 c          676 g          345 t
ORIGIN      
        1 atacgttccc tgctcggtga actgaaactg cgggcgaccc ggattgcccc gggcctttca
       61 agccgcctcg ccggcatgct cgacgcttgc cgccatccca cccggcgggg ggtcatccgg
      121 gcactgctgg cggtgcctgc gctgttgctg ctgtatgtgt tgatcctgat tcccttcacg
      181 ccaagtatca gcaacatccg caaggcgtca tcggagcaac cggcgcaagt cctctcggcg
      241 gatggcagga aactggccga gttcaagcaa tccaaccggc aatgggtggc actctccgag
      301 atttcgccac aggtcatcaa cgccctgatc gccactgaag atcaccgctt ctacgaccat
      361 ttcggtcttg actggcgacg caccgcggct gccgcgctga acaccttccg cggcgatcgc
      421 cagggggggt cgactctcac ccagcagctt gcccgcaacc tctacccgga ggcgatcggc
      481 cgggatcaga cgctgacgcg caagatcaag gaggcgatca cggcgttgaa gatcgaggcc
      541 atccacagca aggacgaaat cctcgaaacc tacctcaaca ccgtgccctt cctctacaac
      601 gcctacggca tcgagatggc ggcacgcacc tatttcgata cctcggccga ccaactcgat
      661 gtactgcaaa gcgccacgct ggtcggcatg ctcaagggca acagttacta caacccggtg
      721 ctcaatcccg agcgatcctt gcagcgccgc aacacggtgc tgaaacagat ggtcaggcgc
      781 gagaaattga ctcaggcgca attcgacgcg ctcagcagga agcccttgcg tatcgacttc
      841 gagcggcaaa cggaacctcc gggaccggct ccgcatttcg ccaggcatct gcgcaagtgg
      901 ctgatcgcct gggccgaccg caacggttac agcatctacg gtgacgggtt ggtggtacgc
      961 accaccatcg actcccggct acaggcggcg gccaaccagg ccgtggcgcg ccagggcaac
     1021 agcttgcaga acatcgccaa cgcggcctgg ggaaaacgct cggcgtggag caccgactcc
     1081 agcctggtgc aggccttcat ccgcgaatcg ccggaatacg ccgccgccat cgccaaaggc
     1141 cggacgcctg agcaggccag gcaggaattg tcggccgaca aggccttcat gcgcgcgctg
     1201 cagcggcaaa agacgcagat ccagatcggt tttctggcca tcgatccgcg caacggccac
     1261 gtcaaggcct gggtcggcag ccgcgacttc gagcaggatg ccttcgacca tgtcgagcag
     1321 gcgcgtcgcc agcccggctc gactttcaaa ccctttgttt acggcgaggc gttccgccag
     1381 ggtgccagcc cggacgaccg attaatcgac caggcagtgg aaatccatgt cggcggcgcg
     1441 gagatctggc ggccaacgga tggcacgccg ccaagcggcg aggccatgcg cctgagcgat
     1501 gggctcgcct attcgaagaa caccatcacc gcgcaactga ttcagcaagt cggtcccgcc
     1561 aaagtggcca aactcgcccg ccacatgggc gtgcgccaga gcaagctcga agccgtgcca
     1621 tcgctcgccc tgggcaccag ccccgtcacc ctcaaggaaa tggttgccgc ctacggcacg
     1681 atcgccaacg gcggcggcta cctcgagccg ctgctggtca gccgcatcga agaccgcgac
     1741 ggccgggtca tcgaagaatt cagcccgcca gtccccgatg ttgcgctcga gcgcgacgtc
     1801 gcctacacgc tgctcgacag cctgcgcggg gtcatcaacc gcggcaccgg caaggatatc
     1861 cgcggccgct atggcatccg cgccgacgtc gccggcaaga ccggcacgac gcaggacaac
     1921 gccgatggct ggttcattct gatgcaccgg cagttggtgg ccggggcgtg ggtcggtttc
     1981 aacgacagcc gcgtcacgct gcgcagcgac tactgggggc agggggcgca cagcgccctg
     2041 ccggtggtcg gcgacttttt cgggaacgcc ttgcgcaccc gcctcatcga tccacgtgcc
     2101 cgcttcgcgg cgccggagga gaccggcctc tgggcacgca tcaagaactg gttccggagc
     2161 gtcttttcgc aggaacccgc cgccgaagcc aggccacagc ggatcgtcaa acccacccga
     2221 aaacagga
//
LOCUS       DAOSIT010000399         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42119_length_2228_cov_8.854119, whole genome shotgun sequence.
ACCESSION   DAOSIT010000399 DAOSIT010000000
VERSION     DAOSIT010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42119_length_2228_cov_8.854119"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLL14_09235"
     CDS             <1..996
                     /locus_tag="PLL14_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG04153.1"
                     /translation="GINGFGRIGRLALRAAWDCPELEFVHINEPNGPVASAAHLLEFD
                     SVHGRWPHAIGVSNGRMQVDGRSIGYSTARDVAGGPWRDAGVDIVLEASGKWRTVDEL
                     SPYLAAGVRKVIVAAPVKGGDTLNIVMGVNDQLYDPARDHIVTAASCTTNCLAPVVKV
                     VHEAIGISHGMITTIHSSTNTQSVHDQMHKDLRRARASSLSLVPTTTGSATAIALIFP
                     ELKGKLDGHAVRVPLLNASLTDCVFEMQRPTSVDEVNGLLKAAADGALKGILGFEERP
                     LVSMDYCGDPRSSIVDAAHTLVINGTLVKIYAWYDNEWGYANRYVELARKLAASL"
     gene            1078..>2228
                     /gene="arsJ"
                     /locus_tag="PLL14_09240"
     CDS             1078..>2228
                     /gene="arsJ"
                     /locus_tag="PLL14_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HOG04154.1"
                     /translation="MSNPSPASSDAARAGLRNYILVTAAYWSDTVTDGATRMLVLFYF
                     YNLGYTPFAVASLFIFYEVFGILTNLFGGYVGARFGLKTTLFIGLGTQLAALSMLAFA
                     PQGWLVVAWVMVSQALSGIAKDMTKMSSKSAVKLIVPEDSSATLYRWVSILTGSKNAL
                     KGVGFFLGGLLLTLLDFQTALKWLVALVGVTLVLAALLMRGGLGEANRKAKFGQMFSR
                     NGAVNKLAAARIFLFGARDVWFVVGLPVFLSSVLGWTFWQSGGFLAVWVIGYGIVQAA
                     TPGVLRSRVEGQHEPDGRTATILAFVLAGLPAGIALGLGAGVDPSTVVVSGLIVFGAV
                     FALNSAVHSYLILAYTDSDKVAMNVGFYYMANAAGRLAGTVLSGALYQWG"
BASE COUNT          348 a          713 c          752 g          415 t
ORIGIN      
        1 ggcatcaacg gtttcggtcg catcggcaga ctggcgctgc gcgcagcgtg ggactgcccg
       61 gagctggagt tcgtgcatat caacgagccg aatgggccgg tagcgtccgc ggcacacctg
      121 ctcgaattcg actcggtgca cggccgctgg ccacatgcca tcggggtcag caacgggcgc
      181 atgcaggtgg acggcaggtc gatcggctac agcactgcca gggacgtagc cggcgggccg
      241 tggcgcgacg ccggggtgga catcgtcctc gaagccagcg gcaagtggcg cacggtcgac
      301 gagttgtcgc cgtacctggc ggcgggggtc aggaaggtga tcgttgccgc gccggtcaag
      361 ggcggcgata cgctgaacat cgtcatgggg gtgaacgacc agctctacga cccggcgcgc
      421 gaccacatcg tcaccgccgc gtcgtgcacc accaactgcc tggcgccggt ggtcaaggtc
      481 gtgcatgagg cgatcggcat ctcgcacggc atgatcacca ccatccacag ttcgaccaac
      541 acccagagcg tgcatgatca gatgcacaag gatctgcgcc gcgcgcgcgc gtcgagcctg
      601 tcactggtgc cgacgaccac cggttcggcg acggcgatcg cgctgatctt cccggaactg
      661 aagggcaagc tcgacggcca cgcggtgcgc gtgccgctgc tcaacgcctc gctgaccgac
      721 tgcgtcttcg agatgcagcg tccgaccagc gtcgacgagg tcaacggcct gctcaaggcg
      781 gcggctgatg gcgcactcaa gggaatcctg ggtttcgagg aacggccgct ggtatcgatg
      841 gattactgcg gcgacccgcg ttcgtcgatc gtcgacgcgg cgcacacgct ggtgatcaac
      901 ggcacgctgg tcaagatcta cgcctggtac gacaacgagt ggggctacgc caaccgctac
      961 gtcgaactgg cgcgcaagct tgccgcgtcg ctgtagccgc gcgctttccg acattgccac
     1021 cggggccggc accgccgcgc cgaccccggt gcactggaac atctcaggag gagcagcatg
     1081 agcaacccat ccccagccag cagcgacgcc gcgcgcgcgg gcctgcgcaa ctacatcctg
     1141 gtcaccgccg cctactggtc agacacggtg accgacggcg ccacgcgcat gctggtgctg
     1201 ttctatttct acaacctcgg ctatacgcct tttgccgtcg cttcgctgtt catcttttac
     1261 gaagtcttcg ggattctgac caatctcttc ggtggctacg tcggcgcccg tttcggcctc
     1321 aagaccacgt tgttcatcgg gctcggtacg caactcgcgg cgctgtcgat gctcgccttc
     1381 gcgccgcagg ggtggctggt cgtggcctgg gtgatggttt cgcaggcctt gagcgggatt
     1441 gccaaggaca tgaccaagat gagttcgaag agcgcggtca agctgatcgt ccccgaggat
     1501 tcgtcggcga cgctgtaccg ctgggtgtcg atcctcaccg gatcgaagaa cgcgctgaag
     1561 ggcgtcggct tcttcctggg tggcctgctg ctgaccctgc tcgattttca gaccgcgctc
     1621 aagtggctcg tcgcgctggt cggtgtcacc ctggtcctcg cagccctgct gatgcgcggc
     1681 ggtctcggcg aggctaaccg caaggcaaag ttcgggcaga tgttctcgcg taacggcgcg
     1741 gtcaacaagc ttgccgcggc gcgcatcttt ctcttcggcg cgcgtgacgt ctggttcgtc
     1801 gtcggtctgc cggtgttcct gtcgagcgtg ctcgggtgga ccttctggca gtccggcggt
     1861 tttctggcgg tctgggtgat cggttacgga atcgtccagg cggcgacgcc cggcgtgttg
     1921 cgcagtcgcg tcgaggggca gcacgaaccc gacgggcgga cggcgacgat cctcgccttt
     1981 gtcctcgccg ggttgccagc cggcattgcc ctggggcttg gcgccggcgt ggacccgagt
     2041 acggtggtcg tcagcggatt gatcgtcttc ggagcggtgt ttgccttgaa ctcggcggtg
     2101 cactcgtacc tgatcctggc ctataccgac agcgacaagg tggcgatgaa cgtcgggttc
     2161 tactacatgg ccaacgccgc cggtcgcctg gccggcacgg tcctgtcggg cgcgctctac
     2221 cagtgggg
//
LOCUS       DAOSIT010000400         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42190_length_2226_cov_7.745739, whole genome shotgun sequence.
ACCESSION   DAOSIT010000400 DAOSIT010000000
VERSION     DAOSIT010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42190_length_2226_cov_7.745739"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="rfbB"
                     /locus_tag="PLL14_09245"
     CDS             <1..803
                     /gene="rfbB"
                     /locus_tag="PLL14_09245"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOG04155.1"
                     /translation="HGPEDFIQTNIVGTFHLLEAVRGYWGSLHDAARQGFRFLHVSTD
                     EVYGSLAREEAAFSETHRYEPNSPYSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGP
                     YHFPEKLIPLMIVNALAGKPLPVYGDGQQIRDWLYVKDHCSAIRRVLEAGRPGETYNV
                     GGWNEQPNLDIVHTVCSLLDELRPRADGKPYCEQIAYVTDRPGHDRRYAIDARKIERE
                     LGWKPAETFATGIRKTVQWYLDNQAWVGNVQSGAYRDWVEKNYGERQG"
     gene            800..1696
                     /gene="rfbD"
                     /locus_tag="PLL14_09250"
     CDS             800..1696
                     /gene="rfbD"
                     /locus_tag="PLL14_09250"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOG04156.1"
                     /translation="MNILLFGKGGQIGWELQRSLAPLGRIVALDWRPEGRLCGDFTDL
                     AGLSACVRAVAPDVVVNAAAHTAVDQAESEPERARTINALAPGVLAAEAARLNAWLVH
                     YSTDYVFDGSGDTAWREGDATGPLGVYGRTKLEGEQAVARHDRHLIFRTSWVFAARGN
                     NFARTMLRLAIEREQIKVVADQIGAPTGAELLADVTAHTIRRVRQQPALAGLYHLVAA
                     GETSWHGYARHVIERARQAGQAIRVAPEAIVAVPSSAYPAPAPRPLNSRLDTRKLQTV
                     FDLRLPDWQDGVDRMLEEILGQ"
     gene            1820..>2226
                     /locus_tag="PLL14_09255"
     CDS             1820..>2226
                     /locus_tag="PLL14_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG04157.1"
                     /translation="MLWWLAYIALGLFSGFFAGMLGIGGGLVMVPTLALMFAAQVAFP
                     ATEVLHLALGTSMAAILFTSLASLREHHRHGAVLWQIVGQITPGILSGTLLGTLFAAR
                     VPARPLALFFTVFVCLVAVQMILNLKPKPSRELP"
BASE COUNT          381 a          762 c          699 g          384 t
ORIGIN      
        1 tccacggacc ggaagacttc atccagacca atatcgtcgg caccttccac cttctcgaag
       61 ccgtgcgcgg ctactggggc agcctgcacg acgccgcgcg gcagggtttt cgctttctgc
      121 atgtgtcgac cgacgaagtc tatggctccc ttgccaggga ggaggcggcc ttcagcgaaa
      181 cgcatcgcta cgagccgaac agcccctact cggcgagcaa ggcggcgagc gaccatctgg
      241 tgcgcgccta tcatcacacc tatggtctgc cggtgctgac caccaactgc tcgaacaact
      301 atggtcccta ccacttcccc gaaaaactga tcccgttgat gatcgtcaat gccctggccg
      361 gcaagcctct gccggtttat ggcgacggcc agcagattcg cgactggctg tacgtgaagg
      421 accattgcag tgcgatccga cgggtgcttg aagccggccg tcccggcgag acctacaacg
      481 tggggggctg gaacgagcag cccaacctcg acatcgtgca cacggtatgc agcctgctcg
      541 acgaacttcg cccgcgtgcc gacggcaaac cctactgcga acagatcgcc tacgtcaccg
      601 accggcccgg ccacgatcgg cgctacgcca tcgacgcccg caagatcgag cgcgaattgg
      661 gctggaagcc cgccgagacc ttcgccaccg gcatccgcaa gacggtgcag tggtacctgg
      721 acaatcaggc ctgggtcggc aatgtgcagt ccggcgccta ccgcgattgg gtcgagaaaa
      781 actacggcga acgccagggg tgaacatcct gctctttggc aaaggcggac agatcggctg
      841 ggaattgcag cgcagcctgg ccccactggg ccgcatcgtc gctctcgatt ggcggccgga
      901 gggcaggctg tgcggcgatt tcaccgacct tgccgggctc tccgcctgcg tgcgtgcggt
      961 cgctcctgac gtggtggtca acgccgccgc ccataccgcc gtcgaccagg ccgagtcgga
     1021 acccgaacgg gcaagaacca tcaacgccct ggctcccggt gtgctggccg cagaagccgc
     1081 ccggctgaac gcctggctgg tccactactc gaccgactac gtgtttgacg gcagcggcga
     1141 cacagcatgg cgagaaggcg acgctaccgg cccgctcggg gtttacggaa ggaccaagct
     1201 cgaaggcgag caggccgtcg cccgccacga ccggcatctg atctttcgta ccagttgggt
     1261 ctttgccgcg cgtggcaaca attttgcccg gacgatgctg cgtctggcga tcgagcgcga
     1321 acagatcaag gtggtcgccg accagatcgg cgccccgacc ggggccgaac tgctggcgga
     1381 tgtcaccgcg cacaccattc gccgcgtccg ccagcagcct gctctcgccg gtctctacca
     1441 tctggtggcc gccggtgaaa ccagctggca tggctacgcc cgacacgtca tcgagcgtgc
     1501 ccggcaggcc ggacaggcca tccgggtggc cccggaagcc atcgtggcgg tgccgagcag
     1561 cgcctacccg gcaccggccc cccgcccgct caactcgcga ctcgacaccc gcaagctgca
     1621 gactgtcttt gacctgcggc tgcccgactg gcaggacggc gtagatcgca tgttggagga
     1681 aattctcggg caatgaggca ggcgtcatca ccgcaggagt cggcagccgc ccgtcctgtt
     1741 cgaccggagt gggagcacgc ggcgggccca gtcccggtgc cgagccaccg ctccggcggt
     1801 tcttgacaag gagtccgagt tgctctggtg gttagcatac attgcgctag gtctgttttc
     1861 cggtttcttc gccggcatgc tcggcatcgg cggcgggctg gtgatggtcc cgacgctggc
     1921 cctgatgttc gccgcgcagg tggcattccc cgcgacagag gtcctgcatc ttgcgctcgg
     1981 cacgtcgatg gcggcgatcc tgttcacctc gctggccagc ctccgtgaac atcatcggca
     2041 cggcgccgtc ctgtggcaga tcgtcggcca gatcacgccg ggcattctct ccggaacctt
     2101 gctcggcacc ctgttcgccg cgcgcgtccc ggccaggccg ctggccttgt ttttcaccgt
     2161 cttcgtctgc ctggtggcgg tgcagatgat cctcaacctg aaaccgaaac cgtcgcgaga
     2221 gttgcc
//
LOCUS       DAOSIT010000401         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42282_length_2222_cov_9.074296, whole genome shotgun sequence.
ACCESSION   DAOSIT010000401 DAOSIT010000000
VERSION     DAOSIT010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42282_length_2222_cov_9.074296"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2222)
                     /locus_tag="PLL14_09260"
     CDS             complement(<1..>2222)
                     /locus_tag="PLL14_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04158.1"
                     /translation="PTGRVLGILVATVSIHSILGEFDRVELRSLGNSGSVSLFDQQRR
                     ILAAFRLVPLADAREVARPPAGEGIEGSFIEAGADGKALLAVYRFVRIGAMEGWSLVV
                     RRERDEALSELSQRAWQLLWFGLLFSLACLLLVTLAARHLTRPIRSLAKVAQRLADGD
                     LGARVFPHPIAGGDEVSALAATFNAMAARIEAWHTTLAREVEMRTRELQDEKRIAQRY
                     LDVAGVMLLVLDHSGRIALINRKGCEVLGWPESQLLGVDWFSRFLPQPLQEQRRDLFN
                     RLLTGDGVLPTRDETTIINASGEERLIDWRNILLHADDGHIQGVLSSGEDITERKQIE
                     RQLHAQQSQLEVLVARRTADLTAALAAAKVADRAKDAFLANVSHELRTPLNAVIGLSE
                     LARRLGTDPRQRDYLDKIANAGKTLCNLINDLLDLSKIAAGRLNFANVTFSLRDLVRR
                     CNSVISHKATDKGLELCERIDDEVPDVLVGDPLRVEQILLNLLSNAIKFTAAGRVAIG
                     IGVCQRQAQRVCLEIIVEDTGIGIGEKDLGLLFEPFSQADATMSRRFGGSGLGLAICR
                     RLVTLMDGEISVSSHLGAGSTFRATLWLQLGNSGDLTASTTPSDDEPLPSTYHDARVL
                     VVEDQPINREIAEALLTEVGITPSVADNGRVALDLLYAGGGQAFDLVLMDIQMPVMDG
                     LTATRELRTHGEFRDLPIIAMAAHTMEHEKEVSRLAGMNDHIGKPFETRAFYQLLARW
                     L"
BASE COUNT          372 a          705 c          730 g          415 t
ORIGIN      
        1 agccaccggg ccagcagttg atagaacgcc cgcgtctcga agggcttgcc gatatgatcg
       61 ttcattccgg ccagtctgct gacttccttc tcgtgttcca tggtgtgtgc ggccatggcg
      121 atgatcggca gatcccggaa ctcgccgtgt gtacgcagct cgcgggtcgc cgtcagaccg
      181 tccatgaccg gcatctggat gtccatcagc accaggtcga aagcctgtcc tccaccggca
      241 tacagcaggt cgagcgcaac cctgccgttg tcggcgacgc tcggggtgat gcccacctcg
      301 gtcagcagcg cctcggcgat ctcgcggttg atcggctggt cttcgacgac caggacgcgt
      361 gcgtcgtgat aggtactcgg caagggttcg tcgtcgctgg gtgtggtgct tgccgtcaga
      421 tcgccggagt tgccgagctg cagccagagc gtggcgcgga aagtgctgcc tgccccgagg
      481 tggctgctga cgctgatctc gccatccatc agcgtgacca ggcggcggca gatggccagg
      541 ccgaggccgc tgccgccaaa gcgccgagac atcgtcgcgt cggcctgcga gaatggctcg
      601 aacagcagac cgaggtcctt ttcgccgatg ccgatgccgg tgtcctccac gatgatctcc
      661 aggcagacgc gttgcgcttg gcgttggcat acgccgatgc cgatcgccac ccgcccggcg
      721 gcggtgaact tgatggcgtt gctgagcagg ttgagcagaa tctgctctac ccgcagcggg
      781 tcgccaacga gcacatcggg tacctcgtcg tcaatccgct cgcagagttc gaggcccttg
      841 tccgtcgcct tgtgcgagat caccgaattg cagcgtcgga ccaggtcgcg caggctgaag
      901 gtcacgttgg caaagttcag tcgtccggcg gcgatcttcg acaggtcgag caggtcgttg
      961 atcaggttgc acagggtctt gccggcgttg gcgatcttgt ccagatagtc gcgttgtcgg
     1021 gggtcggtgc cgaggcgtcg ggccagttcc gaaaggccga tgacggcatt cagcggcgtc
     1081 cgcagttcgt ggctgacgtt ggccaggaag gcgtccttgg cgcggtcggc gacctttgcc
     1141 gcagcgagcg ctgccgtcag gtcggccgtc cgcctggcga ccaggacctc cagttgcgac
     1201 tgctgcgcgt gcaattgtct ttcgatctgc ttgcgttcgg taatgtcctc tccggacgac
     1261 agcacgccct ggatgtgccc gtcatcagcg tgtagcagaa tgttccgcca gtcgatgagg
     1321 cgctcttcac cactggcatt gatgatcgtt gtctcgtcac gcgtgggaag aacgccatcg
     1381 ccagtcagca ggcggttgaa cagatctcgc cgctgctcct ggaggggctg cggcagaaag
     1441 cggctgaacc agtcgacgcc gagcaactgg ctttccggcc agccgagcac ctcgcagccc
     1501 ttgcggttga tcagtgcgat gcgtcccgag tggtccagga ccagcagcat caccccggcg
     1561 acatcgaggt aacgttgtgc gattcgtttt tcgtcctgca gttcgcgcgt ccgcatctcg
     1621 acttcccggg cgagcgtcgt gtgccaggct tcgatccgcg ccgccatggc attgaaggtc
     1681 gccgccagcg ccgaaacctc atcgcctccg gcgatcgggt gggggaacac gcgcgcaccg
     1741 agatcgccgt cggccagtcg ctgcgcgacc ttggccaggc ttctgatcgg gcgcgtcaga
     1801 tgccgtgctg cgagggtcac cagcaacagg caggcgaggc tgaacagcag gccgaaccag
     1861 agcagttgcc aggcgcgttg cgacagttcg ctcaaggcct cgtcgcgttc gcggcggacg
     1921 accaggctcc acccttccat cgcgccgatc cgtacgaagc gatagacggc gagcagggct
     1981 ttgccgtcgg cgccggcttc aatgaagctg ccctcgatac cctcgccagc gggtggccgg
     2041 gcgacttccc gagcatcggc gagtggaacc agccgaaagg ccgccaggat ccgtcgctgc
     2101 tgatcgaaca gcgaaaccga accggaattg cccagcgaac ggagttcaac gcgatcgaat
     2161 tcaccgagta tcgaatggat cgataccgtc gcgacgagta tcccgagcac cctgccggtg
     2221 gg
//
LOCUS       DAOSIT010000402         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42532_length_2212_cov_9.012517, whole genome shotgun sequence.
ACCESSION   DAOSIT010000402 DAOSIT010000000
VERSION     DAOSIT010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42532_length_2212_cov_9.012517"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLL14_09265"
     CDS             <1..281
                     /locus_tag="PLL14_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG04159.1"
                     /translation="VGEQVRFRFFGSSVRRQDEVGTLLEDWAPDELQELDEIQTTLPA
                     EGRATGEVVRVRLQARVTEAGTLELEALPHDGPQHWKVEFDVRGGSGD"
     gene            complement(322..2040)
                     /locus_tag="PLL14_09270"
     CDS             complement(322..2040)
                     /locus_tag="PLL14_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOG04160.1"
                     /translation="MNASVFFLALRRMRAPLIVLIVIYAVSTLGLTLIPGIDAQGRPA
                     PPMSFFHAFYFVSYTASTIGFGELPMAFSEAQRMWVTVVIFLSVIGWSYSIISLLSLV
                     QDKGFQQVLLTARFVRQVRQLGEPFFILCGCGETGLLIARALDRDGLRVVGIERNEAR
                     IQELELEDFAADVLLLCADAAQPQQLLMAGLQHRSCRGVLAVTDDDAANLAVAIASRL
                     INPRLMVVARVASPAVERNMMSFGTHYVVNHFERFADYLAQAIQSPNWYRLVDLLTGL
                     PGQEVPERHDPPAGDWVVCGFGRFGQAVVRNLERHGVHLTVISPEAQLPIAVEHVVGH
                     GTEAEPLRQAGIATAVGIVAASDNDTNNLSIAMTAKEVNPKLFVVLRQNRVANEVLFD
                     AYDANFTMVPSRIVARECLALITSPLLSHFLQRVRNWPDARAAVVARQLEELCGNRVP
                     LVWGVRLNAAEAPAVHKLLMMEQGAMALGMLRRDPAAHQDFLPLLPLLLVRAGIDHEL
                     PAAAMPLEPGDHLLFAGTRAARFAQNLTLDNRNVLDYVLTGHDAPGWLWHLLSRPAQR
                     RQRAPR"
BASE COUNT          408 a          769 c          729 g          306 t
ORIGIN      
        1 tcgtcggcga gcaggtccgc ttccgcttct tcggctcgtc ggtccgccgc caggatgagg
       61 tcggcacgct gctcgaggac tgggcgcccg acgaactcca ggaactcgac gagatccaga
      121 ccaccctgcc ggcggaaggc cgcgccaccg gcgaggtggt ccgcgtcagg ctgcaggcac
      181 gcgtcaccga agccggcaca ctcgaactcg aagccctgcc acacgatggc ccgcagcact
      241 ggaaagtcga gttcgacgtg cgcggcggca gcggcgactg aacggcacgc cagacgcggt
      301 ggcggccagc cgctgccgcg tctagcgcgg cgcgcgctgc cggcgctgcg cgggccggct
      361 cagcaaatgc cacagccagc ccggcgcgtc gtgtccggtg agcacgtagt ccagcacgtt
      421 gcgattgtcg agcgtcaggt tctgcgcgaa cctggccgcg cgcgtcccgg caaacagcag
      481 atggtcgccg ggttcgagcg gcatcgccgc cgcgggtagc tcatggtcga tccccgcgcg
      541 caccagcagc agcggcagca gcggcaggaa gtcctgatgc gccgccggat cgcggcgcaa
      601 catgccgagg gccatcgccc cctgctccat catcagcagc ttgtgcaccg ccggcgcctc
      661 cgccgcgttc agccgcacgc cccagaccag cggcacacga ttcccgcaaa gttcctcgag
      721 ttgtcgggcg acgacggcgg cgcgcgcgtc cggccagttc cgcacccgtt gcagaaagtg
      781 gctcaacaac ggcgaggtga tcagcgccag gcactcgcgc gccacgatgc gcgacggcac
      841 catcgtgaaa ttggcgtcgt aggcgtcgaa caggacctcg ttggcgacgc ggttctggcg
      901 caggacgaca aacagcttcg ggttcacctc cttggcggtc atggcgatcg acaggttgtt
      961 ggtgtcgttg tcgctggcgg cgacgatgcc caccgccgtc gcgatgcccg cctggcgcag
     1021 cggctcggcc tcggtgccgt gccccacgac atgctcaacg gcgatcggca actgcgcctc
     1081 cggcgagatc accgtcaggt gcacgccgtg ccgttcgaga ttgcgcacca ccgcctgccc
     1141 gaagcgaccg aagccacaga ccacccagtc gccggcgggt ggatcgtggc gttcgggaac
     1201 ctcctggccc ggcaggccgg tcagcagatc gaccaggcgg taccagttcg gcgactggat
     1261 cgcctgcgcc agatagtcgg caaaacgctc gaagtgattg accacatagt gcgtgccgaa
     1321 agacatcatg ttgcgctcga ccgccggact ggcgacgcgc gcgacgacca tcaggcgggg
     1381 attgatcagg cggctggcga tcgccaccgc caggttggcc gcgtcgtcgt cggtcaccgc
     1441 cagcacgccg cgacaactgc gatgctgcag cccggccatc aacaactgct gcggctgcgc
     1501 cgcatcggcg cacagcagaa ggacgtcggc ggcgaaatcc tcgagttcga gttcctggat
     1561 acgcgcttcg ttgcgctcga tgcccaccac ccggaggccg tcgcggtcga gggcgcgcgc
     1621 gatcagcaaa ccggtctcgc cacaaccgca gaggatgaag aaaggttcgc cgagttgcct
     1681 gacctggcgc acgaagcgtg cggtcagcaa cacctgctga aaacccttgt cctgaaccag
     1741 cgacagcagg ctgatgatcg agtacgacca gccgatcacc gaaagaaaga tgaccaccgt
     1801 gacccacatc cgctgcgcct cggaaaaggc catcggcaat tcaccgaaac cgatggtgct
     1861 ggcggtatag ctgacgaaat agaaggcgtg gaaaaagctc atcggcggcg ccggccgacc
     1921 ctgcgcgtcg atgcccggaa tgagcgtcag ccccagcgtc gacaccgcgt agatgacgat
     1981 cagcacgatc agcggcgcgc gcatccgccg cagggcgagg aagaagacgc tggcgttcat
     2041 ggccgcgcgg ctcgcggtgc agcgcgcgtc agcgccgcaa catcacggtt tccgcgatca
     2101 gcagcaccac cgagacgacg ttggcgaaca gcgcgccgcc gctcagcgaa accacgctgg
     2161 tcgtgacatc cgcggacatt ccctcgccgg tgacatgcac ggcgatcgcc ca
//
LOCUS       DAOSIT010000403         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42560_length_2211_cov_7.410946, whole genome shotgun sequence.
ACCESSION   DAOSIT010000403 DAOSIT010000000
VERSION     DAOSIT010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42560_length_2211_cov_7.410946"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLL14_09275"
     CDS             complement(<1..464)
                     /locus_tag="PLL14_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOG04161.1"
                     /translation="MRRCFAVLAVAFLSACAGLAGLAQKPEVSVAGLNLVQFGLFEQR
                     FALKLRIQNPNDVELPINGLNFEIELNGQPFIKGLSDKSVTIPRFGEGVLEVMATSTL
                     GSALKQLRELQKGGRERVDYRIVGRLNVAGSVSLPFERRGDLQMPAVESPPR"
     gene            complement(461..1339)
                     /locus_tag="PLL14_09280"
     CDS             complement(461..1339)
                     /locus_tag="PLL14_09280"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG04162.1"
                     /translation="MRLGFIGLGVMGRPMALHLLRAGHELAVWARRPASCQPLLDAGA
                     TGVATPADVARRADVVFTMLTAGVDVQQVTFGPDGLAAGFAAHSVLIDMSTIAPQLAR
                     TLAQDLLARCAVEMLDAPVSGGAQGAIAATLAIMVGGKATVLERVRPLLEVLGRTLVH
                     VGEHGAGQVAKACNQMLMVAAIQAVAEALHLSRAAGVAPAGVRQALAGGSAGSRVLDV
                     MGGRMVDRDFVAGVEARLHHKDYAIVIGEAHRLGVPLPVSTQVGQQLNALMAMGWGQN
                     DTATLLRVLEAMEGAV"
     gene            complement(1339..>2211)
                     /locus_tag="PLL14_09285"
     CDS             complement(1339..>2211)
                     /locus_tag="PLL14_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOG04163.1"
                     /translation="IGFIGLGIMGRPMALNLLRAGHQLTVWARRAESMQPLLDAGAGA
                     AANPAGLAEVADVVISMVADAPDVEQVMLGEQGVASAGRPGLIAVDMSTIRPAAARAM
                     AAHLLARGIDFLDAPVSGGEVGAIAGTLSIMVGGGVAAFETVRPAFACMGHNIVHVGD
                     SGAGQVAKAANQIVTGVGVLTVAEALNFAARSGVDPARVREALLGGFAYSKILENHGQ
                     RMLDRNFKPGFKSWMHQKDMNIVMQSAHELGLCLPVSAATAQMYNAMVGSGLGEEDSI
                     AILKLLERLSGVDN"
BASE COUNT          386 a          803 c          678 g          344 t
ORIGIN      
        1 ttcctcggcg gactctcgac cgccggcatc tgcagatcac cgcgccgttc gaatggcagc
       61 gaaacactgc cggcgacgtt gaggcgcccc acgatacggt agtcgacgcg ctcgcggcct
      121 cccttctgca actcacggag ttgtttcagc gcgctcccga gcgtgctggt cgccatcact
      181 tccagcaccc cctcgccaaa acgtggaatg gtgacgctct tgtccgacag ccccttgatg
      241 aagggctgtc cattgagttc gatctcgaaa ttcagaccgt tgatcggcaa ctcgacgtcg
      301 ttcgggttct ggatgcgcag cttgagggcg aagcgctgtt cgaacaggcc gaattgcacc
      361 agattcagac cggcaacgct gacttccggt ttctgcgcga ggcctgccag accggcacag
      421 gcgctcagaa aagcgaccgc cagcacggca aaacatcttc tcataccgcc ccttccatgg
      481 cttcaagaac gcgcaacagc gtcgctgtgt cattctgccc ccagcccatc gccatcaggg
      541 cgttgagttg ctgtccgacc tgcgtggaca ccggcaaggg cacgccgagt cgatgcgctt
      601 cgccgatcac gatcgcgtag tccttgtgat gcaggcgcgc ctcgacgccc gcgacgaagt
      661 cgcggtcgac catcctgccg cccatcacgt cgagcacccg gctgccggcc gaaccgcccg
      721 ccagggcctg tcgcaccccg gccggggcga caccggcggc gcggctcaaa tgcagcgcct
      781 cggccaccgc ctggatggcg gccaccatca gcatctgatt gcaggccttg gcgacctgcc
      841 cggcgccatg ctcgccgaca tgcacgaggg tcctgccgag gacttccagc agcggccgca
      901 cgcgttcgag caccgtcgcc ttgccgccga ccatgatcgc cagcgttgcc gcgatggcgc
      961 cttgcgcgcc cccggacacc ggcgcatcga gcatctcgac agcgcagcgg gccagcagat
     1021 cctgcgccag ggtgcgcgcc agttgcggtg cgatggtgct catgtcgatc agcaccgaat
     1081 gcgccgcaaa ccccgccgcc aggccatcgg gcccgaaagt cacctgctgc acgtcgaccc
     1141 cggcagtgag catggtgaac accacgtcgg cacgccgcgc gacgtcggcg ggcgtggcga
     1201 cgcctgtcgc cccggcgtcg agcaaaggct ggcaggatgc cggccgccgc gcccagaccg
     1261 ccagttcgtg cccggcacgc agcagatgca gcgccattgg ccgccccatc acgccgaggc
     1321 cgatgaagcc gaggcgcatc agttatcgac gcccgacagg cgttcgagca gcttgaggat
     1381 ggcgatcgag tcctcctcgc cgaggcccga accgaccatc gcgttgtaca tctgcgccgt
     1441 cgcagccgaa accggcaggc acagccccag ttcatgcgca ctctgcatga cgatattcat
     1501 gtccttctgg tgcatccagg atttgaagcc cggtttgaag ttgcggtcga gcatccgctg
     1561 tccgtgattc tccagaatct tcgaataggc aaaaccgccg agcagcgctt cgcgcactct
     1621 ggccgggtca acgccgctcc tggctgcgaa gttgagcgct tcggcaacgg tcaggacgcc
     1681 gaccccggtg acgatctggt tggccgcctt ggccacctgc ccggcacccg aatcaccgac
     1741 atgcacgatg ttgtggccca tgcaggcaaa cgccggccgc accgtttcga aggcggcgac
     1801 gccgccaccg accatgatcg acagcgttcc ggcgatcgcg ccgacctcgc cgccggacac
     1861 cggcgcatcg agaaaatcga tgcccctggc gagcaggtgc gcagccatcg cgcgtgccgc
     1921 cgcgggccgg atggtgctca tatcgacggc aatcaagcct ggccggccgg cactcgccac
     1981 cccttgctcg ccgagcatca cctgctcgac atcgggcgcg tcggcgacca tcgagatcac
     2041 cacgtccgcg acctcggcga gaccggcggg attcgccgcg gcgccggcgc cggcatccag
     2101 caggggctgc atcgactccg cgcggcgcgc ccagacggtc agttggtgac cggcacgcaa
     2161 caggttcagc gccatcgggc gccccatgat acccagaccg atgaatccga t
//
LOCUS       DAOSIT010000404         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42636_length_2208_cov_9.118439, whole genome shotgun sequence.
ACCESSION   DAOSIT010000404 DAOSIT010000000
VERSION     DAOSIT010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42636_length_2208_cov_9.118439"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1210
                     /gene="hemH"
                     /locus_tag="PLL14_09290"
     CDS             107..1210
                     /gene="hemH"
                     /locus_tag="PLL14_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOG04164.1"
                     /translation="MPRYLQEPAYRHGSPALTAVLLINLGTPTAPDTQAVRAYLKEFL
                     ADPRVVEIPRPIWWLILNGIILNIRPRRSAEKYASVWTPEGSPLKVHVERQAKLLRGF
                     LGQAGQRVLVDYAMRYGEPSIPTTLSRLKAEGSSRILLLPLYPQYAASTTATALDAAG
                     AWLKRVRNQPEIRTVRSFADHPGYIAALAASVREHWTSHRRPDSSYRLLMSFHGLPRY
                     TLDKGDPYHCECQKTGRMLAEALGLGPEQYQICFQSRFGRAQWLQPYTAPTLQALAAQ
                     GVRRIDAICPGFPADCLETLEEVAIEGKCEFLRAGGKEFYYLPCLNERDDWIHALADI
                     VAQHLQGWPTQVSPDTASLELSALRAKALGASS"
     gene            complement(1289..>2208)
                     /locus_tag="PLL14_09295"
     CDS             complement(1289..>2208)
                     /locus_tag="PLL14_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOG04165.1"
                     /translation="IMAQEHIDLPRGTIKATVLVETILATFEMEEILYELREHSAGLN
                     AGRWDYIFSCIKKFKKNADFCLANRGAITMEVPFMRSYALQLVKICHKRGAPAMGGMS
                     ALIPIKNDPVANEKALAGIRHDKTRDANDGFDGGWVAHPGLVAIAGEEFQKVLGERPN
                     QWEKQRDESFTAADFLNFQPSQPITEAGVRNNINVGIHYLGSWLAGNGCVPIHNLMED
                     AATAEISRSQVWQWVVSPKGVLDDGRKVTAEMVRGMIAEELRKVKATVSAQGEDTATY
                     DQAAVIFDKMSLTPEYPEFLTLPLYEAMD"
BASE COUNT          394 a          724 c          654 g          436 t
ORIGIN      
        1 taggcgtcat cggccccacc cgcatggcct acgagcgagt gatcccgatc gtcgagatca
       61 ccgccaagct gctttcttcg gcactcactt tccaggccaa ctgctgatgc cacgctacct
      121 tcaggagcca gcctaccgcc acggttcgcc cgctctgacc gcggtgctac tgatcaacct
      181 ggggacgccg acggcgccgg acacgcaggc ggtgcgcgcc tatctgaagg aattccttgc
      241 cgacccgcgc gtcgtcgaga tcccgcgtcc catctggtgg ctgatcctga acggcatcat
      301 tctcaacatt cgacctcgac ggtcggccga aaaatacgcg tcggtatgga ctcccgaagg
      361 ctcgccgctg aaggttcatg ttgaacgcca ggccaagctg ttgcgaggtt ttctcggaca
      421 agccgggcag cgggtgctgg tcgactatgc gatgcgttac ggtgagccgt cgattccgac
      481 tacgctttcc cgcctcaagg ccgaaggcag cagccgcatc ctgctgctgc ccctctatcc
      541 acaatacgcc gccagcacca ccgccaccgc cctcgacgcg gccggcgcct ggctgaagag
      601 ggtacgcaac cagccggaaa tacggaccgt caggagtttt gctgatcatc cgggctacat
      661 cgcagcactt gcagccagtg ttcgcgagca ctggaccagc caccggcggc cggacagttc
      721 atatcgcctg ctcatgagct tccatggact gccgcgctac acgctggaca agggcgaccc
      781 ctatcactgc gagtgccaga aaaccggccg catgctggcc gaggcactgg gtctgggtcc
      841 ggagcagtac cagatctgtt tccagtcgcg tttcggacgt gcgcagtggc tgcagcccta
      901 caccgcgcca acgctccagg ccctcgccgc gcagggtgtg cggcggatcg atgcgatttg
      961 tcccggcttt ccggcagact gcctcgaaac gctcgaagaa gtcgccatcg aaggcaagtg
     1021 cgagttcctg cgcgccggtg gcaaggagtt ctattacttg ccctgcctca acgagcgtga
     1081 tgactggatt cacgcactgg ccgacattgt cgcccagcat ctgcaaggct ggcccactca
     1141 ggtttcaccc gacaccgcca gcctggaact cagcgccttg cgcgccaagg ctctcggcgc
     1201 gtcttcctga cgaacagcca cttggctccc gccagcctcc cgcacgcgct tgccggcctg
     1261 gcttccgacc ggcaagcacc aggaacaatc agtccatcgc ctcgtacaac ggcagggtca
     1321 ggaactcggg gtattccggg gtcagcgaca tcttgtcgaa gatgaccgcc gcctgatcgt
     1381 aggtcgcggt atcttcaccc tgggcactga ccgtcgcttt caccttgcgc agttcctcgg
     1441 cgatcatgcc gcggaccatc tcggcggtga ccttgcggcc gtcgtccaga acacctttcg
     1501 gggacaccac ccactgccag acctgcgagc gcgagatctc ggcagtagca gcgtcttcca
     1561 tcaggttgtg gatgggcacg caaccgttgc cggccagcca gctacccagg tagtggatgc
     1621 cgacgttgat gttgttgcgc acgccggctt cggtgattgg ttgcgaaggc tggaagttga
     1681 ggaagtcggc ggcggtaaac gattcgtcgc gctgtttctc ccactggttg gggcgctcgc
     1741 cgagcacctt ctgaaattcc tcgccggcaa ttgcgaccag acctgggtgg gccacccaac
     1801 cgccgtcgaa accgtcattg gcatcacgcg tcttgtcgtg gcggataccg gcgagcgcct
     1861 tttcattggc taccggatcg ttcttgatcg gaatcagggc actcatgcca cccatcgccg
     1921 gcgccccgcg cttgtggcag atcttgacca gttgcagggc gtagctgcgc atgaagggga
     1981 cttccatggt gattgctccg cggttggcga ggcagaaatc cgcgtttttc ttgaacttct
     2041 tgatgcatga aaagatatag tcccagcggc cggcgttgag gccggcagaa tgctcgcgca
     2101 gttcgtagag gatttcctcc atctcgaagg tggcgagtat cgtctcgacc agtaccgtgg
     2161 ccttgatcgt tccccgcggc agatcgatgt gctcctgggc catgatga
//
LOCUS       DAOSIT010000405         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42752_length_2204_cov_8.429502, whole genome shotgun sequence.
ACCESSION   DAOSIT010000405 DAOSIT010000000
VERSION     DAOSIT010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42752_length_2204_cov_8.429502"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLL14_09300"
     CDS             <1..894
                     /locus_tag="PLL14_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOG04166.1"
                     /translation="LLAAGDVPAATEEIVMSELRERMSNAMCLRGMAARTQEAYIGAL
                     VELAKYYHRSPADLRVEEVQAYLLHLIRDKKLAYASVNQASCAFRFLYRRVLGRPEAG
                     FDIPMAKVPQRLPQILSREEVARLIDSARTLRGRTLLMTTYAAGLRVSEVCALQVGDI
                     ESAADRMCLKVRQGKGGKDRYTLLSPHLLAALRTYWRDTRPRLWLFANRAGTGPMEIQ
                     TAQRIYYAARNAAGIAPEGGIHGLRHAFATHLLEAGVDLYSIGRLLGHGHLSTTSRYL
                     HLARSKLTGNPSPLDLLDPPS"
     gene            905..2062
                     /locus_tag="PLL14_09305"
     CDS             905..2062
                     /locus_tag="PLL14_09305"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOG04167.1"
                     /translation="MTLAEIFRRHGPAYLAKHALSSAKAKVWRAIVACRTAILGGHVE
                     TCDGCGVTRHVYHSCRNRHCPQCQTRAKEHWLAKRRQELLPVPYFHLVFTLPHALHAL
                     IALRPRVIYEVLFAVASATLSEFGANPRWLGGKLAFSLVLHTWKQDLGRHVHVHALVA
                     GGALTPEGKWKSPKRGFLFPVRALSTVFRGKFIAALSEARGSGRPLVAELADEKVWRA
                     LRQQLLAHDWVVYAKQPLGGPEQVLEYLGRYTHRVAISNERLVGMDETTVRFRVRATP
                     GKRVLCVPAEEFIDRFLLHVLPSGFKRIRHYGLLAPAAKATRLALARQALSVPEPDPV
                     IKATVEDFLQRVSRAEWARCTHCHEGRFVPTAPIPPLRQAAPQSASVRGPP"
BASE COUNT          338 a          750 c          740 g          376 t
ORIGIN      
        1 ctcctcgctg cgggggacgt ccccgcagca accgaggaga tagtcatgag tgagctgcgt
       61 gaaagaatga gcaacgccat gtgcctgcgg ggaatggcgg cacggaccca ggaagcctac
      121 atcggtgcgc tggtcgaact ggcgaagtac taccaccgat cgccggcgga cttgcgtgtc
      181 gaggaagtcc aggcctacct gctgcacctg attcgcgaca agaagctcgc ctacgcgagc
      241 gtcaatcaag cctcgtgtgc cttccgcttc ctctatcggc gggtgctcgg tcgcccggaa
      301 gccggcttcg acatcccgat ggccaaggtg ccccagcgcc tgccgcagat cctctcgcgc
      361 gaagaagttg cgcgcctgat cgactcggcg cgaaccctgc gcgggcgaac gctattgatg
      421 accacctacg ccgccggctt gcgggtctcg gaagtctgcg ccctgcaggt gggggacatc
      481 gagagcgccg ccgatcgcat gtgcctcaag gtgcgtcagg gcaaaggcgg caaggatcgc
      541 tacacgctgc tgtcgccgca cctgctcgcc gccttgcgca cctactggcg cgacacccgg
      601 ccgcggctct ggttgttcgc caaccgggcc ggcaccggcc cgatggaaat ccagaccgcg
      661 cagcggattt actacgccgc gcgtaacgcc gcgggcatcg cccccgaagg cggcatccac
      721 ggtcttcgcc atgccttcgc cacgcacctg ctcgaagccg gcgtcgatct ctacagcatc
      781 ggccggctcc tcggccatgg tcatctgtcg accacctcgc gctatctgca tctggcacgc
      841 tcaaagctga ccggcaaccc ctcgcccctg gacctgctcg acccgcccag ttgagccacc
      901 gccagtgacc ctggccgaga tcttccgccg gcacgggccg gcgtacctcg ccaagcacgc
      961 tttgtcgtcg gccaaggcca aggtctggcg ggcgatcgtc gcctgtcgca cggccatcct
     1021 cggcggccac gtcgagacct gtgacggctg cggggtgacg cgacacgtct atcactcgtg
     1081 ccgcaaccgc cactgtccac agtgccagac ccgagccaag gagcactggc tggcgaaacg
     1141 gcgccaggag ctgttaccgg tgccatattt ccatctggtg ttcaccctgc cgcacgcgct
     1201 gcatgcgctg atcgcgctac gcccgcgggt gatctacgag gtcctctttg ctgtcgcgtc
     1261 ggccaccctc agcgaattcg gcgccaatcc ccgctggctg ggcggcaagc tggcgttctc
     1321 gctggtcctg cacacctgga agcaggacct cgggcgacat gtgcatgtac acgccctggt
     1381 cgccggcggg gcgttgacgc ccgagggcaa atggaagtcg ccgaagcgcg gcttcctgtt
     1441 cccggttcgt gcgctgtcaa cggtctttcg cggcaagttc atcgcggcgt tgagtgaagc
     1501 cagaggctcg gggcggccgc tggtggcaga gctggccgac gagaaggtct ggcgggccct
     1561 gcgccagcag ttgctggcgc acgactgggt ggtctacgcg aagcagccgc tcggcgggcc
     1621 ggagcaggtc ctcgaatacc tgggtcgcta tacccatcgg gtggcgatct ctaacgagcg
     1681 tctcgtgggg atggacgaga cgacggtccg tttccgggtg cgcgctacgc cgggtaagcg
     1741 cgtgctctgc gtgccggcgg aggagttcat cgaccgcttc ctgctccacg tgctccccag
     1801 cggtttcaag cggattcgcc actacggcct gctggctccg gcggcgaagg cgacgaggtt
     1861 ggcgctggca cggcaggcct tgtcggtacc ggaacccgat ccggtgatca aggcgacggt
     1921 ggaggacttc ctgcagcgcg tcagccgcgc ggagtgggcg cgctgcacgc attgccacga
     1981 aggacggttc gtgccgacgg cgccgattcc gcccctgcgt caggcggcgc cgcagagcgc
     2041 atcggtgcgc ggaccgccgt gagcacggct cgagtgggcc aggggcttcc cgtgggccat
     2101 ggcggttcag gctgccaggg ggtggttcgt cggcagcagg ccgacgtcac ggttccggcg
     2161 ctggcttggg cttgccagag tgccctgttc gttcctgtga cact
//
LOCUS       DAOSIT010000406         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42812_length_2202_cov_5.716348, whole genome shotgun sequence.
ACCESSION   DAOSIT010000406 DAOSIT010000000
VERSION     DAOSIT010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42812_length_2202_cov_5.716348"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLL14_09310"
     CDS             complement(<1..713)
                     /locus_tag="PLL14_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOG04168.1"
                     /translation="MAWGMMTVELLGGLAIFLFGMLQMEEGLKAVAGERMKGILAKLT
                     VNRFMGVGTGALVTAVIQSSSVTTVLVVGFISAGLMTLSQSVGVIMGANIGTTITAQI
                     VAFKITKAALGMIAVGFAMLFIARSDRTKHYGEMLMGLGMVFFGMHVMSEAMEPLRSY
                     QPFLDLMKSMDNPLVGVLIATAFTALIQSSSASTGIVIVMAGQGFISLQAGIALAFGA
                     NIGTCATALLAVIGKPREAV"
     gene            1041..>2202
                     /locus_tag="PLL14_09315"
     CDS             1041..>2202
                     /locus_tag="PLL14_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOG04169.1"
                     /translation="MLVTAQHNAYPYALGGGPSEGWAEAQAGNGAALRGVRHGILAGE
                     YLRGRGHFMEWARGAGDFIALSSIDWELLLRVLIQCGLFSMSAFFSGSETALFSLSRI
                     DLQKLRNSRNPQSESIHAMLDEPRRLIVSLLCGNELVNIAAAANMTMILVRAFGEEDA
                     GWINVVVMVPLLLLFGEVTPKTIAVNFPVKFATLLSARFLPKWIFIVTPLRNVVRMVA
                     DRVTTLVVGEHVGRENILKADELRTLMADSEETGVIQASERVLIDKVLEAAETDVARI
                     MTPGPRIRFLDGDLPIAELVERFRSFRHPRVPVYRGHWDNVIGFLHAEDLLRRVRSEG
                     GLAAIELRDILKPAHFVPPTKKVDEMLEYFQRFDTRAAVVIGEYGEVLGIVTI"
BASE COUNT          409 a          677 c          697 g          419 t
ORIGIN      
        1 accgcttcgc gcggcttgcc gatcaccgcc agcagcgccg tcgcgcaggt gccgatgttg
       61 gcgccgaaag ccagggcgat gcctgcctgc aggctgatga aaccctgtcc ggccatgacg
      121 atgacgatgc cggtcgaagc cgacgaggac tggatcaggg cggtgaaggc ggtggcgatc
      181 aggacgccga ccaaggggtt gtccatgctc ttcatcaggt cgaggaaagg ctggtagctg
      241 cgcagcggct ccattgcctc gctcatcacg tgcatgccga agaacaccat gcccaggccc
      301 atcagcattt cgccgtagtg cttggtccgg tcgctcctgg cgatgaacag catcgcaaag
      361 ccgaccgcaa tcatccccag cgccgccttg gtgatcttga aggcgacgat ctgggcggtg
      421 atcgtggtgc cgatgttggc gcccatgatc acaccgaccg actgcgagag cgtcatcagc
      481 ccggccgaga tgaagccgac caccagcacc gtcgtcaccg aggaagactg gatcaccgca
      541 gtgaccagcg cgccggtgcc gacgcccatg aagcggttaa ctgtcagctt ggcgaggatt
      601 cccttcatcc gctcgccggc gacggccttg aggccttcct ccatctgcag catgccgaag
      661 agaaagatcg ccaggccacc gagcagttcg acggtcatca ttccccacgc cattccgccg
      721 gcatcgtcgc cggtggccgc caacgccggc caggccagcg tatacaacga tgtcccgaga
      781 gcatacaagg tgagcggaag agctacacga gtgcagggat tcagatgcgt tttcagcatg
      841 gatcacacct ccgatggtta caacccggtc aaagatacaa taaattctga ctgcatacat
      901 ccatttgtct gtcgcgacag gcagctcggt atcagcgagg tggcacgcgt attcccggca
      961 actgcggtct cggcatgccg gcaggcgaac tctcggcgca ggattgcggg tcttggcaag
     1021 tccggagacg aagccggttt gtgcttgtta ctgcgcaaca taacgcttat ccttacgcgc
     1081 tgggtggcgg gccgtccgag gggtgggctg aggcgcaagc aggtaatggc gcagcgcttc
     1141 gcggtgtgcg gcacggcatt cttgccggtg aatatctgcg ggggcggggg catttcatgg
     1201 aatgggcaag aggggctggc gacttcatcg cgctgtcgtc gatcgactgg gaactgctgc
     1261 tgcgcgtgct gattcagtgc ggcctgttca gcatgtcggc ttttttttcc ggatccgaaa
     1321 ccgcgctttt ctcactgagc cgcatcgacc tgcagaaact gcgtaattcg cgcaatcctc
     1381 aatccgagag catccacgcc atgctcgacg agccgcgacg cctgatcgtc tcgctgctgt
     1441 gcggcaacga gctggtcaat atcgcggcgg cggcgaacat gacgatgatc ctggtgcgcg
     1501 cctttggcga ggaagacgcc ggctggatca acgtggtggt gatggtcccg ttattgctgc
     1561 tgtttggcga ggtgacgccg aagaccatcg ccgtcaactt tccggtcaag ttcgccacgc
     1621 tgctttcggc gcgcttcctg cccaagtgga tcttcatcgt caccccgctg cgcaacgtcg
     1681 tgcgcatggt tgccgaccgg gtcacgacac tcgtcgtcgg cgagcacgtc ggccgcgaga
     1741 acatcctgaa ggccgacgag ttgcgtacgc tgatggccga cagcgaggaa accggggtca
     1801 tccaggcgtc ggaacgggtg ctcatcgaca aggtgctcga agcggcggag accgacgtgg
     1861 cacggatcat gacccccggc ccgcgcatcc gcttccttga cggcgacctg ccgatcgcgg
     1921 aactcgtcga gcgcttccgc agttttcgtc atccacgtgt cccggtttat cgcggtcact
     1981 gggacaatgt catcggcttc ctgcatgccg aggatctgct gcgccgcgtc cgcagtgaag
     2041 gcggcctggc cgcgatcgaa cttcgcgaca ttctgaaacc ggcgcacttc gttccgccga
     2101 ccaagaaggt cgatgagatg ctggagtact tccagcgttt cgacacgcgc gcggcggtgg
     2161 tgatcggcga gtacggcgag gtcctgggca tcgtcaccat ca
//
LOCUS       DAOSIT010000407         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42863_length_2200_cov_6.400932, whole genome shotgun sequence.
ACCESSION   DAOSIT010000407 DAOSIT010000000
VERSION     DAOSIT010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42863_length_2200_cov_6.400932"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2200
                     /locus_tag="PLL14_09320"
     CDS             <1..>2200
                     /locus_tag="PLL14_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04170.1"
                     /translation="DLNPVAVLINKAMIEIPPKFAGKPPVNPDVKRKADLAGRDWKGA
                     QGLAEDVRYYGQWMRDEAEKRIGHLYPKVEVTAAMVQERPDLKQYAGKEAYVEPVILP
                     LLPQGEGRGEGGVGYRFTVKVGKPKDAEAAKNGTKLSRGANFKCLMSGTPMAGDYIKA
                     EGKAGRMGARLMAIVAEGDRGRVYLAPTPEMEAIALTAQPEWRPETPLPADMRSHWTP
                     PYGLTTYADLFTPRQLVALTTFSDLVQEARERVKLNAIAAGLPDDGKALDKVGTGATA
                     YADAVGVYLGFGVSKSADYWSSICTWRSDPKNLGVGDVFSRQAVPMAWDYAEGNPFST
                     SSGNYLVSLDWIVRVINYLPSVARGFVQQADAQNQSIGDSKVVSTDPPYYDNIGYADL
                     SDFFYVWLRRSLKPVIPELFATLVVPKAEELVATPYRHGSKEKAERFFLDGMTQAMHR
                     LAEQAHPAFPVTIYYAFKQAENDGKDGTTNTGWDTFLAAVIEVGFAISGTWPMRTELS
                     NRMIGSGTNALASSIVLVCRQRSATAPTATRREFVTALKAELPAALAHLQRGNIAPVD
                     LAQAAIGPGMAVYTRYAKVLDAEGKSIPVRAALALINQTLDEALAEQEGDFDADTRWA
                     LTWFEQTGFAEGDYGVAEQLSKSKNTAVGGLAEAGILLSNAGKVRLLKPDELPANWDP
                     TTDSRLTVWEMVHQLIRVLEASGETAAAALVAKLGSQAETARELCYRLYTL"
BASE COUNT          451 a          697 c          661 g          391 t
ORIGIN      
        1 gacctcaatc cggtggcggt gctgatcaac aaggcgatga tcgagattcc gccgaagttc
       61 gccggcaagc cgccggtcaa tcccgacgta aagagaaaag ccgacctcgc cggacgtgac
      121 tggaaagggg cgcagggcct cgccgaggac gtgcgctatt atggccagtg gatgcgggac
      181 gaggccgaga agcgcatcgg ccatctctac cccaaggtcg aggtcacggc ggcaatggtg
      241 caggaacggc cggacctgaa acaatacgcg ggcaaggaag cgtatgtcga gccggtcatt
      301 cttccccttc tccctcaggg agagggccgg ggtgaaggtg gggttggcta ccgcttcacg
      361 gtgaaggtgg gcaagccgaa ggatgcggaa gcggcgaaga acggcaccaa gctgtcgcgc
      421 ggggcaaact tcaagtgcct gatgtcgggg acgccaatgg ccggcgacta catcaaggcc
      481 gagggcaagg ccggccgcat gggcgcacgg ctgatggcca tcgtcgccga aggtgatcgt
      541 gggcgggtct atctcgcgcc aacgcctgaa atggaggcga ttgccctgac cgcgcaaccg
      601 gaatggagac ctgagacgcc gttgcctgcg gacatgcgct cgcactggac gcccccctac
      661 gggctgacca cctacgccga tctcttcacc ccccgccaac tcgttgcgct gacgaccttc
      721 tccgatctgg tgcaggaagc gcgtgagcgg gtgaaactca atgccatcgc tgctggcctg
      781 cccgacgacg ggaaagccct ggataaagtc ggcactggag ccacggcgta tgcggatgcg
      841 gtgggtgtgt acctgggctt tggagtcagt aagtcagccg attattggtc gagtatttgc
      901 acttggagat ctgacccaaa aaacttgggc gttggggatg tgttctcgcg ccaagctgtc
      961 cccatggcat gggactatgc ggaagggaat cccttttcga catcttctgg aaattacctg
     1021 gtgtcccttg actggatagt aagagtcatc aactacctac ctagcgttgc gagagggttt
     1081 gtccagcaag ctgatgccca aaatcagtct attggcgata gtaaagttgt ctctacagac
     1141 cctccgtact acgacaacat tggctatgcc gacctgtcag acttcttcta cgtctggctt
     1201 cgccgttcgc tgaagccagt catccccgaa ctcttcgcaa ctttggttgt gcccaaagcc
     1261 gaggaactcg tcgcaacacc ctaccgccac ggcagcaagg aaaaggctga gcggtttttt
     1321 ctcgacggca tgactcaggc gatgcaccgc ctcgccgaac aggcgcaccc cgcctttccg
     1381 gtcactatct actacgcttt caagcaggcc gaaaacgatg gcaaggacgg tacaactaat
     1441 accggttggg acaccttcct cgctgcggtg attgaagtcg gcttcgccat cagtggcacc
     1501 tggccgatgc gcacggaatt gagcaaccgg atgattggct ccggcaccaa cgccctcgcc
     1561 tccagcatcg ttctcgtctg ccgccagcgc tccgctactg cccccacagc cacacgccgc
     1621 gaattcgtca cggcactgaa ggccgaactc cccgcggcgc tggcccatct ccagcgcggc
     1681 aacattgcgc cggtggacct cgcacaggcc gcgatcggcc ccggcatggc cgtctacacg
     1741 cgctatgcga aagtcctcga cgctgaaggc aaatcaatcc cggtgcgcgc cgcgctggcg
     1801 ctgatcaacc agaccctcga cgaagcgctg gccgagcagg aaggcgactt cgacgccgac
     1861 acccgttggg cgctgacctg gttcgagcag accggctttg ccgagggcga ttacggtgtc
     1921 gccgaacagc tctccaaatc caagaacacc gccgtcggcg gcctggccga agccggaatc
     1981 ctgctctcca atgccggcaa ggtgcggctg ttgaagcccg acgaactgcc tgccaactgg
     2041 gaccccacga ccgactcccg cctgaccgtc tgggaaatgg tgcaccagtt gattcgcgtc
     2101 ctcgaagctt ccggcgaaac cgccgctgcc gcactggttg ccaaactcgg cagccaggcc
     2161 gaaaccgccc gcgaactctg ctaccgcctg tacacgctgt
//
LOCUS       DAOSIT010000408         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42913_length_2198_cov_10.298647, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000408 DAOSIT010000000
VERSION     DAOSIT010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42913_length_2198_cov_10.298647"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1513
                     /gene="nifL"
                     /locus_tag="PLL14_09325"
     CDS             23..1513
                     /gene="nifL"
                     /locus_tag="PLL14_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HOG04171.1"
                     /translation="MHPQVFQHTVNQADMAISITDVRGNILYVNPAFTRVTGYGSDEA
                     IGKNQSILSNQSMPRAFYQSLWQRISHGEPWGGRLVNRRKDGSKYLAELSISPVVNGA
                     GEVLNYLGMHRDITEVQRLEYQVRNQKALIESVVDAAPVVIALLDIDDRVVLDNHAYK
                     MLQADLGMAEPAPMLMTAVRASLELDSGRGRRTSGYLFLDREVRLDPPGGKQPRWFSC
                     SGSRVREDAGTAEAFLAGQGRDYLLLVAKEITSLRAQQEKARVAALRAVMADEDRVSS
                     LRESLAAATFRLEGPLNMMASVVGMLARRKGETDPMSVALADAVTAGQKALDELRSMI
                     PGKTREAIGPVNLNELLRDVLDLSTGRLLAAGITVNWKPQAMLPTLKGAPNKLRSLFK
                     ALIDNAIEAMNTRGCRERELTVVSKARLGGIEVIIADTGPGIVGTQQLKVFEPFHTTK
                     RGGGHLGTGLPSAQQVAAEHGGFIEIEPATARGCRVRVLLPLDRRP"
     gene            1510..>2198
                     /locus_tag="PLL14_09330"
     CDS             1510..>2198
                     /locus_tag="PLL14_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG04172.1"
                     /translation="MSENRPVSLMHSQFETLLRVSQVLARAVDFSQSLREVLRALEIT
                     GHLSRGMLGVVDPDAGDLSVHLVHGLEHEEFQAVRYQAGEGLLGRILESGRSCAVVRL
                     GDEPHFLNRLGFYERDLPFIAAPIHVGGTLQGVLAVQPDAPDDGRLAERTRFVEMLAN
                     LIGQNLRLAVEMTQEDKSPDGERDSLRRSVRQRPGFGNLVGHSVSMLRIFDQIRMVSK
                     WPTTVLIRGETG"
BASE COUNT          399 a          713 c          713 g          373 t
ORIGIN      
        1 aacggaaaag ctctctccga gcattcaccc gcaggtcttc cagcacaccg tcaatcaggc
       61 cgacatggcg atctcgatca ccgacgtgcg tggcaacatc ctgtacgtca atccggcctt
      121 tacccgcgtc accggctacg gcagcgacga ggcgatcggc aagaaccagt cgatcctgtc
      181 caaccagagc atgccgcgcg cgttctatca gtcgctctgg cagcggattt cgcacggcga
      241 accgtgggga ggccggttgg tcaatcgccg caaggacggc agcaagtacc tggccgagtt
      301 gagcatctca ccggttgtca atggcgccgg cgaagtgctg aactacctcg gcatgcaccg
      361 cgacatcacc gaagtccagc gcctggagta ccaggtcaga aaccagaagg cgctgatcga
      421 gtcggtggtc gatgccgcgc cggtggtgat cgccctgctc gacatcgacg accgcgtggt
      481 actggacaac catgcctaca agatgctgca ggccgacctg gggatggccg agccggcgcc
      541 gatgttgatg accgcggttc gcgccagtct cgaactcgat tcggggcgcg gacgacggac
      601 ctccggttac ctttttctcg atcgcgaagt ccggctcgac ccgcccggcg gcaagcaacc
      661 gcgctggttc tcgtgttcgg gaagccgtgt gcgcgaggac gccggcacgg ctgaagcctt
      721 cctggccgga cagggtcgcg attacctgct gctggtggcg aaggaaatca ccagcctgcg
      781 cgcgcagcag gaaaaggccc gcgtcgccgc cctgcgggcg gtgatggcgg atgaggatcg
      841 ggtttcctcc ctgcgcgaga gtctcgccgc cgccaccttc cgcctcgaag gaccgctgaa
      901 catgatggcc tcggtggtcg gcatgctggc cagacgcaag ggcgaaacgg acccgatgtc
      961 ggtggcgctg gcggatgcgg tcaccgccgg tcagaaggca ctcgacgaac tgcgctcgat
     1021 gattcccggc aaaacgcgcg aggcgatcgg cccggtcaat ctcaatgaac tgctgcgcga
     1081 tgtcctcgat ctgtcgaccg gtcgcctgct cgccgccggg atcaccgtca actggaaacc
     1141 gcaggccatg ttgccgaccc tgaagggggc accgaacaag ctgcgttcgc tgttcaaggc
     1201 gctgatcgac aatgccatcg aagcgatgaa cacgcgcggc tgccgggaac gcgaactgac
     1261 ggtggtcagc aaggcgcgtc tgggtggcat cgaggtgatt atcgccgata ccggacccgg
     1321 catcgtgggc acccagcagt tgaaggtctt cgagcccttt cacaccacca agcgcggtgg
     1381 cggccatctc ggcaccggcc tgccttcggc acagcaggtc gcggccgaac acggcggttt
     1441 catcgagatc gaaccggcga cggcgcgcgg ctgccgcgtg cgcgtattgc tgccactgga
     1501 ccgacgacca tgagcgagaa ccgtccagtg tccttgatgc actcccagtt cgaaaccctg
     1561 ttacgggtct cgcaagtcct cgcgcgcgcg gtcgatttct cgcagagtct gcgcgaagtg
     1621 ctgcgcgcgc tcgaaatcac cggtcatctg agccggggca tgctcggcgt ggtcgatccc
     1681 gacgccggcg acctgagcgt gcacctcgtg catggactcg aacacgagga attccaggcg
     1741 gtgcgctacc aggccggcga aggtctgctc ggccggatcc tggaaagcgg ccgcagttgc
     1801 gcggtcgttc gccttggcga cgagccacac ttcctgaacc gcctgggctt ctacgaaagg
     1861 gacctgccgt tcatcgccgc accgattcat gtcggcggta cgttgcaggg cgtgctggca
     1921 gtgcagcccg acgcgccgga cgatggtcgg ctcgcagagc gcacccgttt tgtcgaaatg
     1981 ctggccaatc tgatcggcca gaatctccgg cttgcggtcg aaatgacgca ggaggacaag
     2041 tcgcccgacg gggagcgcga cagccttcgc cgctcggttc gccagcggcc cggcttcggc
     2101 aacctcgtcg gccactcggt gtccatgctg cggatcttcg atcagatccg catggtttcc
     2161 aaatggccga caacggttct gatccgcggc gaaaccgg
//
LOCUS       DAOSIT010000409         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_42995_length_2195_cov_7.283645, whole genome shotgun sequence.
ACCESSION   DAOSIT010000409 DAOSIT010000000
VERSION     DAOSIT010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42995_length_2195_cov_7.283645"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLL14_09335"
     CDS             complement(<1..1683)
                     /locus_tag="PLL14_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG04173.1"
                     /translation="MTLLERLGTALRQRQRWLLIALLAVVHLALVAGAATTISLLCWL
                     VNVGLFILWQPFIYAERKVDFSGLAVIALMLVCGAAWYGWWLLIVWVVMLAALVGGRV
                     MFIDHRPTRIFYLVAFAYLLAALLFWLVPKVVPHAALTGPSLDREFAWGMPLFLLAMT
                     LLPLSRESDRPGSGMVDLFYSLFMFLLIAVLVLGSLAFMLLRQSGYFEAVLNTLISIA
                     LLLLLIGWTWNTRPGFTGIDVFFSRYLLSIGLPFEKWLHRLTTLASSESEPERFLSQA
                     LAEMLDLPWVDGGNWTAGTRHGEFGKTSRFCHEFPGQPLLLTLHTRHKLSPALIWHFH
                     LLAQLANEHYLAKLRTRELQQVSYLRAIHETGARLTHDVKNLLQSLNNLCFLAQTMSG
                     NDDDRLDLLLQRQLPQITQRLQQTLSKLQKPQTEAGGVLPAEMWWTIQQQRYATAPVT
                     FAAVEFQADALLPTALFDSITDNLLQNALLKGRVESGLRVHLTLAADASRLCVCDTGS
                     PIGEAVLGDLLRAPVASENGLGVGLYHAAQQAESGGYELRLTSNVAGKVCFEL"
     gene            1871..>2195
                     /locus_tag="PLL14_09340"
     CDS             1871..>2195
                     /locus_tag="PLL14_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG04174.1"
                     /translation="MSKQQSGFTLVEIAIVLVIIGLLLGGVLKGQELINSAKVKNMVG
                     DFRTVSSLAYGYQDRFKALPGDQTQAQLDLAFGAGVAAACAPAAAGQCTQNNGRIDGA
                     WNAAAV"
BASE COUNT          442 a          667 c          728 g          358 t
ORIGIN      
        1 cagctcgaaa cacaccttgc cggcgacgtt gctcgtcagc cgcagttcgt agccgccgct
       61 ttccgcctgc tgcgcggcgt ggtagagccc gacgccgagg ccgttctccg aggccaccgg
      121 cgcccgcagg aggtcaccga gtaccgcctc gccgatcgga ctgccggtat cacagacgca
      181 caagcgcgag gcgtcggcgg cgagtgtcag gtgtacccgc aggccgcttt ccacgcgtcc
      241 cttgagcagc gcgttctgca gcaggttgtc ggtgatgctg tcgaaaagcg ccgtcggcag
      301 cagcgcgtcg gcctggaact ccacggcggc gaaggtcacc ggtgccgtcg cgtagcgctg
      361 ttgctgaatg gtccaccaca tctccgccgg caggacgccg ccagcctcgg tctgtggttt
      421 ctgcagcttg ctcagtgttt gctgcaggcg ctgggtgatc tgcggcaact gccgctgcaa
      481 cagcaggtcg agccggtcgt cgtcgttgcc gctcatggtc tgagcgagaa aacacagatt
      541 gttcagcgat tgcagcaggt tcttcacatc atgcgtgagg cgggcgccgg tttcatggat
      601 ggcgcgcagg tagctcacct gctgcaactc gcgagtgcgc agcttggcaa ggtaatgctc
      661 gttggccaac tgcgccagca agtggaaatg ccagatcagg gccggactga gcttgtggcg
      721 ggtgtgcagg gtcagcagca ggggttggcc gggaaactca tggcagaaac gcgaggtctt
      781 gccaaactcc ccgtggcgcg taccggcggt ccaattgccg ccatcgaccc acgggaggtc
      841 gagcatctcg gccagagcct gcgacaggaa acgttccggt tcgctctcgc tgctggccag
      901 cgtcgtcagc cgatgcagcc acttttcaaa gggcaggccg atgctcagca ggtagcgcga
      961 gaagaagacg tcgataccgg tgaagccggg acgcgtgttc cacgtccagc cgatcagcag
     1021 cagcagcagc gcgatcgaga tcagcgtgtt gagtacggcc tcgaagtatc cggactgccg
     1081 caggagcatg aacgccaggc tgccgagtac caggacggca atcagcagga acatgaacag
     1141 actgtagaag aggtcgacca tcccgctgcc gggtcggtcg ctctcgcgcg agaggggtag
     1201 cagggtcatc gccagcagga acaacggcat tccccaggcg aattcgcgat ccagcgaggg
     1261 gccagtcagg gccgcgtgcg gcactacctt gggcaccagc cagaagagca gtgccgccag
     1321 caggtaggca aaggcgacca ggtagaagat gcgcgtcggg cgatggtcga tgaacatcac
     1381 ccgtccacca accagcgcag ccagcatgac cacccagacg atcagcagcc accagccgta
     1441 ccaggccgcg ccgcagacga gcatcagggc aatcaccgcc agcccgctga aatcgacctt
     1501 tcgttcggcg tagatgaacg gctgccagag aatgaacaac ccgacattca ccagccagca
     1561 cagcaggctg atcgtcgttg cggcgccggc gaccagcgcc aggtgtacca ccgcgagcag
     1621 ggcgatgagc agccagcgct ggcgttgacg cagtgcagtg cccaggcgtt cgagaagggt
     1681 cacgcggcgc tcctggcggt tgccggtagc cgagtgacag gcgtctgcat cagtccgact
     1741 ccgggcaatt gtgtcgaaaa ctcgacagat cggcaggctt tgaagactgg atcggtggaa
     1801 ttttccgggc acgaaacgtg ctattagcca atatcatcga tgacacggac tggagaacca
     1861 aatgaaccag atgagcaagc aacagtcggg ttttaccctg gtagagattg cgattgtact
     1921 cgtcatcatt ggcctgctgc ttggcggggt gctgaagggg caggaactga tcaacagcgc
     1981 caaggtgaag aacatggtcg gggatttccg gaccgtgtcg agcctggcct atggctatca
     2041 ggatcgcttc aaggcgctgc ccggtgacca gacgcaggcg cagctagacc tggccttcgg
     2101 tgccggcgtg gccgccgcat gcgcgccggc cgctgcgggc cagtgtacgc agaacaacgg
     2161 gcgcatcgac ggcgcctgga atgccgccgc ggtga
//
LOCUS       DAOSIT010000410         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43039_length_2193_cov_9.118803, whole genome shotgun sequence.
ACCESSION   DAOSIT010000410 DAOSIT010000000
VERSION     DAOSIT010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43039_length_2193_cov_9.118803"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLL14_09345"
     CDS             complement(<1..290)
                     /locus_tag="PLL14_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG04175.1"
                     /translation="MAQVLIVDDEMGIRELLSEILADEGHSVWLAENATAARKLRAEK
                     RPDLVLLDIWMPDTDGISLLKEWSASGLLTMPVVMMSGHGTIDSAVEATRIGA"
     gene            complement(293..>2193)
                     /locus_tag="PLL14_09350"
     CDS             complement(293..>2193)
                     /locus_tag="PLL14_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04176.1"
                     /translation="PGVLIYAVSVQFVTKSIETWFDVRVEKALEAGLNLGRSALDSLL
                     EDLAVKGQTMALELSERSEAQRRLELGRLREQNGVQSVALFSTAGELLATATGELHTL
                     LPLQPAADQLRRARTERRYAVIESNGEKDLTLRVLVAVSPAGLGVETRILQLTEPVPT
                     SLALNADSVQTVYADYQELSLGREGLTRIYAMTLTLTVLLALFTAIALAFVLARRLSA
                     PLSILAEGTQAVAAGDYTPRQAVYSRDELGVLTRSFKQMTSQLDEARRDNERHRAELE
                     AARAYLESILANLSAGVLVFDHRFVLRTVNEGALTILSDDFAALLGEAAEAWPRQQVI
                     GQAILDAFAEDERTEWQEQIELEHPGGLPQILLLRGTQLPAGSHGGYVVVFDDVTRLI
                     AAQRSAAWGEVARRLAHEIKNPLTPIQLSAERLQMKLADHLNGVDAQVLARSTQTIIT
                     QVQAMKRMVNDFSDYARLPAPELAAVDLNALVLEVLGLYETSRATIETKLAPRLPPVW
                     GDTTQLRQIIHNLLRNAEDAQEALDAPFIAISTRRAEGMAEMVVRDQGPGFPPEIIAR
                     VFEPYVTTKARGTGLGLAIVKKIVDEHHGRVRINNRQPAGAEVSILLPLASLPTRKRR
                     SSTLPSET"
BASE COUNT          396 a          689 c          701 g          407 t
ORIGIN      
        1 gcaccgatgc gggtcgcttc caccgccgaa tcgatcgttc cgtgtccgga catcatcacc
       61 accggcatgg tcaaaaggcc gctcgcggac cactccttga gcaacgaaat accgtcggta
      121 tccggcatcc agatgtccag cagaaccaga tcaggccgct tttcggcgcg cagcttgcgg
      181 gccgcggtag cgttttcggc cagccacacc gagtgaccct cgtcggccag aatctcggag
      241 aggagttcgc ggattcccat ttcgtcatcg acgatcagta cttgcgccat ggtcaggtct
      301 cggaaggtag tgtgcttgag cgccgcttgc gggtgggcag ggaggccagc ggcaacagga
      361 tgctgacttc ggcgccggcc ggctgacggt tgttgatcct gacacggccg tgatgttcgt
      421 cgacgatctt cttgacaatc gccaggccca ggccggtacc gcgcgccttg gtggtgacgt
      481 agggttcgaa aacccgggca atgatttcgg gcggaaaacc tggcccctga tcccggacga
      541 ccatctcggc catgccttct gcgcgtcgcg tgctgatcgc aatgaacggt gcatccagag
      601 cttcctgcgc gtcttcggcg ttgcgcagca gattgtggat gatttgccgc aactgcgtgg
      661 tatctcccca gaccggcggt agccgcggcg cgagtttggt ctcgatcgtc gcccgcgagg
      721 tctcataaag gccgagtact tcaagcacca gggcgttgag atcaacggcc gccagttcag
      781 gagcaggcag gcgcgcgtag tcactgaaat cattgaccat gcgcttcatc gcctgcacct
      841 gcgtaatgat cgtctgcgtg gagcgtgcga gcacctgcgc gtcgacgccg ttcaggtggt
      901 cggcgagttt catctgcagg cgctcggcag acaactggat cggcgtcagc ggattcttga
      961 tctcgtgtgc gagccgcctc gccacttctc cccaggcggc actgcgctgg gcggcgatga
     1021 gccgggtcac gtcgtcaaac acgaccacat acccgccatg cgagccggcc ggcaactgtg
     1081 tgccgcgcag cagcagaatt tgcggcagtc cgccgggatg ttcgagctcg atctgttcct
     1141 gccattcggt gcgttcgtct tcggcaaagg catcaaggat ggcctggcca atcacttgct
     1201 gccgcggcca ggcttcagcc gcctcgccaa gcagagcagc aaaatcgtcc gacaggatag
     1261 tcagcgcacc ttcgttgacc gtacgcagga cgaagcgatg gtcgaaaacc agtacccctg
     1321 cagaaaggtt ggccaggatc gattcgaggt aggcgcgcgc ggcttcgagc tcggcacgat
     1381 ggcgctcgtt gtcccgtcgc gcctcgtcga gctggctggt catctgcttg aacgatcggg
     1441 tcaggacgcc caactcatcg cggctataga cggcctgccg gggcgtgtaa tcgccggcgg
     1501 ccaccgcctg ggtcccctcg gcgaggatcg acagcggtgc cgacaggcga cgtgccagta
     1561 cgaaagccag cgcgatggcc gtgaacaggg ccagcagtac agtcagggtc agcgtcatcg
     1621 cgtagatgcg ggtgaggccc tcgcggccca gcgagagttc ctgataatcg gcgtataccg
     1681 tctgtacgct gtccgcgttc agcgccaggc tggtcggcac cggctcggtg agttgcagga
     1741 tgcgggtttc gacgcccagg ccggccggag agaccgccac cagcacacgc agggtgagat
     1801 ccttctcgcc attgctttcg atgaccgcgt agcgacgctc ggtacgtgcc cgccgcaact
     1861 ggtcggcggc cggctgcagc ggcaacaagg tatgcagttc gccggtcgcc gtggccaaca
     1921 gctcgccagc ggtggagaac agggcgaccg actgcacgcc attctgctcg cggaggcgac
     1981 caagctcaag gcgccgctgt gcttctgaac gttcactaag ttccaaggcc atggtctgcc
     2041 ctttgaccgc caggtcttca agcaatgaat cgagagcgct gcgaccgaga ttcaggcccg
     2101 cttcgagggc cttctcgacg cgcacgtcga accaggtttc aatgctcttg gtcacgaact
     2161 gtaccgacac ggcgtagatc agcacgccgg gca
//
LOCUS       DAOSIT010000411         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43086_length_2191_cov_7.880618, whole genome shotgun sequence.
ACCESSION   DAOSIT010000411 DAOSIT010000000
VERSION     DAOSIT010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43086_length_2191_cov_7.880618"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLL14_09355"
     CDS             complement(<1..1007)
                     /locus_tag="PLL14_09355"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOG04177.1"
                     /translation="MEGIHFLDLFQGIATLMASEPKIMFGRIFLMLLGFLLIYLGSRN
                     ILEPLLMIPMGLGMSSVNAGVMFLDGNKMGTLFVDPLMSDPIELMNIMQIDWLQPIYT
                     LTFSNGLIACLVFMGIGVLLDVGYVMARPFQSMLIALFAELGTIAVFPIAVWLGLNPG
                     QAAAVATIGGADGPMVLFTSLVLAKDLFVPITVVGYLYLGLTYGAYPYLIKWLVPEHI
                     RGISMAADRGPKISRSQKLTFAVVACTLLCLLFPVAAPLFFSLFLGVAVRESGIDAFT
                     KLLGEVFLYAATFFLGLTLGVLCEANTLLEPTVLKLLLLGMLALTISALGGLLGGYIL
                     Y"
     gene            complement(1099..1497)
                     /locus_tag="PLL14_09360"
     CDS             complement(1099..1497)
                     /locus_tag="PLL14_09360"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359172.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOG04178.1"
                     /translation="MPRQFKVTVNGREYEVSVLELTAGQAPQQSAPAAPMAVAAAGSA
                     TSAAPAAAVAPRPAAAAGAGDELAGMGGVVVEVDVKVGQTVAVGERLLVLEAMKMKTP
                     VMATRAGQVTRVLVAPGDAVEGGQPLLTIA"
     gene            complement(1517..1846)
                     /locus_tag="PLL14_09365"
     CDS             complement(1517..1846)
                     /locus_tag="PLL14_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04179.1"
                     /translation="MVSDAIFKALQVYGIAIVISLVVAVLIKVLVAVTGRIEKAKPID
                     VPTGTVCPIGLGVPDEDVAALSAAIFVAIGAHHILHIAPTSHGWAAGARAAQHSHTPG
                     PTGRGPR"
     gene            complement(1870..>2191)
                     /locus_tag="PLL14_09370"
     CDS             complement(1870..>2191)
                     /locus_tag="PLL14_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOG04180.1"
                     /translation="MCSQEMGADMVYAWPTAEIAVMGAEAAVKILYRKELDQAEDRQA
                     KAAELAQEYRDEFASPYVSASNFYITDVIEPQDTRWMVALALRKILDKRELRPAKKHG
                     NIPM"
BASE COUNT          436 a          676 c          688 g          391 t
ORIGIN      
        1 aaatagagaa tgtaaccgcc gagaaggccg ccaagggccg agatcgtcag ggccagcata
       61 cccagcagga gcagtttgag aaccgtcggc tccaacaggg tattggcctc gcagagcacg
      121 cccagagtca ggccgaggaa gaaggttgcc gcgtagagaa acacctcccc gagcagtttg
      181 gtaaacgcgt cgataccgct ttcgcgcacc gcgacgccga gaaacagcga gaagaacagc
      241 ggcgcagcca ccggaaacag cagacatagc agcgtgcatg ccaccacggc aaaggtgagc
      301 ttctgcgagc ggctgatctt cggtccgcgg tcggcggcca tgctgatccc gcggatgtgc
      361 tcgggtacca gccacttgat caggtaaggg taagcgccat aggtcagacc gagatagagg
      421 tagccaacga ccgtgatcgg cacgaagaga tccttggcca gtaccagaga ggtgaatagc
      481 accatcgggc cgtcagcacc gccgatggtg gcgacggcag cggcctggcc cggattgagg
      541 cccagccaga cggcgatcgg aaagacggcg atggttccca gttcggcgaa gagggcgatt
      601 aacatgctct ggaaggggcg cgccatcacg tagcccacat cgagcaggac accgatcccc
      661 atgaatacca ggcaggcgat cagaccattg ctgaaggtca gcgtgtagat cggttgtagc
      721 cagtcgatct gcatgatgtt catcagttcg atcgggtcgg acatcaaggg atcgacgaac
      781 agtgtcccca tcttgttgcc atcgaggaac atcaccccgg cgttgaccga cgacatgccc
      841 agacccatcg ggatcatcag caagggttcg aggatgttcc tggagcccag atagatcagc
      901 aggaagccga gcaacatcag gaagatgcgc ccgaacatga ttttcggctc ggacgccatc
      961 agcgtggcaa tgccctggaa cagatcgaga aaatgaatgc cttccatacg cttcagtcct
     1021 cgcgccaggc ggcaggaatc atccgcccgc gcatcgagtt gcagacgaca ggaactcgct
     1081 cctgcctgtt ggcctggctc aagcgatggt cagcagcggc tgaccgccct cgaccgcatc
     1141 gccgggtgcg accagcacgc gggtgacctg acccgcgcgc gtcgccatga ccggggtttt
     1201 catcttcatg gcttcgagga ccagcagccg ctcaccgacc gccacggtct ggccgacctt
     1261 gacgtcgacc tcgaccacga cgccacccat gccggccagt tcgtcgccgg ctccggcagc
     1321 cgcggctggt cgcggcgcca ctgccgcggc cggtgcggcg ctggtggcgc tccctgcggc
     1381 tgctaccgcc atgggagccg ccggagcgga ttgctgcggt gcctggccgg cagtgagttc
     1441 gagaacgctg acctcgtact cgcgtccgtt gacggtcacc ttgaattgtc tgggcattgt
     1501 ttgctccttc taccttctat ctggggcctc ggcccgtggg ccccggcgta tgggaatgct
     1561 gggcggcgcg tgcgcctgct gcccagccat ggctggttgg tgcaatgtgc aagatgtggt
     1621 gcgcgccgat ggccacgaag atcgccgccg acagcgcggc gacgtcttca tccggaacgc
     1681 cgagtccgat ggggcagacg gttcccgtcg gcacgtcgat cggcttcgcc ttttcgatcc
     1741 ggccggtcac cgcaaccagc accttgatca ggacggcaac caccagcgag atgacaatgg
     1801 cgatgccgta gacttgcagc gccttgaaaa tggcgtcaga caccatgggg gtcttccttc
     1861 cgttgccgat tacatgggaa tgttgccgtg ctttttcgcc ggcctgagtt cacgcttgtc
     1921 gagaatcttg cgcagcgcga gggcgaccat ccagcgcgta tcctgcggtt cgatcacatc
     1981 ggtgatgtag aaattgctgg ccgatacata gggcgacgcg aattcgtcac ggtattcctg
     2041 cgccagttca gcagcctttg cctggcgatc ctcggcctga tcgagttcct tgcggtagag
     2101 gatcttgacc gcggcctcgg cgcccatcac ggcgatctcg gcagttggcc aggcgtaaac
     2161 catgtcggct cccatttcct ggctgcacat g
//
LOCUS       DAOSIT010000412         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43134_length_2189_cov_6.972352, whole genome shotgun sequence.
ACCESSION   DAOSIT010000412 DAOSIT010000000
VERSION     DAOSIT010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43134_length_2189_cov_6.972352"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLL14_09375"
     CDS             <1..299
                     /locus_tag="PLL14_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04181.1"
                     /translation="LEALHTHLLLIYGYGIGGEERLPIGLELFHLLLALKDGAQLAGA
                     GQQGVFAHLEIFTQRLAQEDARELHGWHPGDEASVFRVYVEARDGRQILVRKAA"
     gene            296..2068
                     /locus_tag="PLL14_09380"
     CDS             296..2068
                     /locus_tag="PLL14_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04182.1"
                     /translation="MNAKTIVENVLTPARARDLWTVNYFPALPFTPQHFEVGGLLPAM
                     LYMARWGYRRGKGRFIETFGPKDAQSKARPPRLIDVIQGLLTRAGDAFYGFDGQNGPA
                     MLADLLLTYCLENKSHELGHQEVVQRVFPTHYLASWVDLPETVAHLRGVPELLTVVLA
                     WQDKGEALAPETGRSRFPVGVGFANNRLLAQFGRHMSVRGLAANQAADHFQEETADDL
                     GVDELLAIRLAQTCSHAPDRLRGSEQSQIANRWPVAKTAAKHLRGDLAIFIEVFGVAL
                     PRQAFLPMLEAGISLGMVNLLLSSAVCLSEWERTGSIPKDPTPMPLFVDCSHGLDNKL
                     RNASEAAMGEALARYERLPVLMMLARVLDDRARFDRILRNSLPSASPDPTARIDLLGE
                     VYHERHNRATGLLNGLEEDCLRLADALESEASDLARALRTDTMNPALRLAEALVELHG
                     HKQQGSKFTEALDSSLMPNRPNGLAFKRRVSRNEAGTKKAVDLRSIVLSHALLDFLVH
                     RHLRKEGEGKAARPLTLRRFLDILREHYGLYVDREPPGMSVPQDLLRANKQWLERRLR
                     DLGLLVGVNDAESMKQLRARFDVA"
BASE COUNT          418 a          663 c          687 g          421 t
ORIGIN      
        1 ggctggaagc gttgcatacc catctgctgc tgatttatgg ctatggaatc ggcggtgagg
       61 aacgcctccc gatcggactg gaactcttcc acctgctgct ggcgctgaag gacggcgcgc
      121 agcttgccgg cgccggccag caaggcgtgt tcgcgcatct cgaaatcttc acccagcgcc
      181 tggcccagga ggatgcccgc gaactgcatg gctggcatcc cggcgatgaa gccagcgtgt
      241 tccgggtgta cgtcgaggcg cgggatggac ggcagatact ggtgaggaaa gcggcgtgaa
      301 cgcgaaaacc atcgtcgaga acgtattgac tccggcacgc gcccgagacc tttggacggt
      361 caactatttt ccggcgctgc ctttcacccc gcagcatttc gaagtgggtg gtctgctgcc
      421 tgcgatgctc tacatggcgc gatggggcta tcggcggggc aaaggtcgct tcatcgagac
      481 ctttggcccg aaggatgcgc agagcaaagc gcggccgcca cgccttattg acgtgataca
      541 gggcttgctg acgcgtgcgg gtgacgcgtt ctacgggttt gatggccaga acggcccggc
      601 catgctggcc gacttgttgc tcacgtactg tcttgagaac aagtcgcacg aactcggaca
      661 tcaggaagtg gtgcaacgag tttttccgac gcattacctg gcgagctggg tggatctgcc
      721 cgaaacggtc gctcatttgc gcggcgttcc ggagctgctg acagtcgtgt tggcctggca
      781 ggacaaggga gaagcacttg caccggaaac gggccggagt cgtttcccgg taggcgtcgg
      841 ctttgccaat aaccgcttgc ttgcgcagtt tggtcgccac atgtcggtac gtggtctcgc
      901 cgccaaccag gcggccgatc atttccagga ggaaactgcg gacgacctcg gggtagatga
      961 attgctggcg attcgtctgg cgcagacttg tagccatgcg cccgacagac tcagggggag
     1021 tgagcagtcg cagatcgcca atcgctggcc tgtcgccaag accgccgcca agcatttgcg
     1081 cggcgacctg gcgattttca tcgaggtctt tggcgtcgcg ctgccgagac aggctttcct
     1141 gccaatgcta gaagcgggca tcagcctcgg catggtcaac ctgcttctct cctctgcggt
     1201 ctgtttgtcg gaatgggagc ggaccggcag cataccgaaa gacccgacgc cgatgccact
     1261 gttcgtagac tgctcgcacg ggctggacaa caagctacgc aatgcttccg aagcggcgat
     1321 gggcgaggca ctggcccgct acgagcggct gccagttctg atgatgctgg cccgtgtgct
     1381 cgacgatcgt gcgcgctttg accgtattct gcgcaacagt ctaccgagcg cgagccctga
     1441 cccgactgcg cgcatcgacc tgctgggcga ggtctaccac gaacgtcata accgggccac
     1501 cggcctcctc aatggactcg aggaggattg cctacggctg gccgatgcgc tggaatcgga
     1561 ggcatcggat ctggcgcggg ctttgcgcac agacaccatg aacccggcgc tacggctggc
     1621 ggaagcgctg gtcgagttgc acgggcataa gcaacaaggc tcgaaattca cggaggcact
     1681 ggacagcagc ctgatgccca accgccccaa cggcctcgcc ttcaaacgcc gcgtttcccg
     1741 caacgaagct ggcaccaaga aggcagtaga cctacgttcc atcgtgctct cccacgccct
     1801 gctcgacttc ctggttcatc gccacctgcg caaggagggc gaaggcaaag ccgcgagacc
     1861 gctcacgctg cgcaggttcc tcgatatcct gcgcgagcac tatggccttt acgtggaccg
     1921 cgaaccaccg ggcatgtccg taccccagga cctgctgcgc gccaacaagc agtggctgga
     1981 acgtcgactg cgcgatctcg ggttgctggt gggggtgaac gatgctgaat cgatgaagca
     2041 gttgcgcgca cgatttgatg tagcatgatt ggcttcaatc gtatcgaaac cggctacaac
     2101 catgctcgca tcgcttctct tcggcgatta ccgtcagaaa gtgctcaccc gcttactgtt
     2161 gcacccggaa cagcgctacc acgtgcgcg
//
LOCUS       DAOSIT010000413         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43162_length_2188_cov_7.321144, whole genome shotgun sequence.
ACCESSION   DAOSIT010000413 DAOSIT010000000
VERSION     DAOSIT010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43162_length_2188_cov_7.321144"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            541..2049
                     /locus_tag="PLL14_09385"
     CDS             541..2049
                     /locus_tag="PLL14_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOG04183.1"
                     /translation="MGLSSLVRVIRELLHEAARDLAPTLIVVVLFQVFFIQRMPDSPV
                     ALIGGFAIVLLGLALFVKGLDAAIFPAGETMAFDFARRGSFLWLMLFAFCISFASVAA
                     EPALMAVAYKAEAVSGGSMGALTLRMVAALGVGLAVMLGVWRIVLGHSIGNYLIPGYL
                     LVVVLTVFSPPQLVGLAFDSGGVVASTVTAPLLTALGVGLATSIRGRNPMLDGFGLIA
                     FGALAPMIIIQIYGILFFVPAAQGNAVLMMPSDDQHQYAALELLIDFLIMVRNLLPII
                     AVVLFSSFVILRRPLADPKGLAVGMVLVAVGLFLFDEGLQIGLFPLGDEMTNGLMRDD
                     VPMWAVYLYGFLIGFATTMAEPALIALSIKADEVSLGQLKGLWLRTLVSIGVGIGIVI
                     GCARIIDGINIAYWLIPGYLLVLAMTKFAPDFIVPIAYDCGGVTTSTVTVPLVTALGV
                     GLAERTPGRDPMIDGFGLIAFASLLPMIIVMSYGMLATWLLRSRTLKEKQRP"
BASE COUNT          346 a          716 c          688 g          438 t
ORIGIN      
        1 tacgcactct gcgcgccgtc ggcggtcagc caggcgatgc tcagcggcaa cagcatcagg
       61 ccgccgaagc cggcgatcag gatgccgaga atgaacagca ctggagcgaa acgatcgatc
      121 atgcaagcgc ctcgtcaccc gtggaaaatc gggccagcgc cgtgcggcgg tgtacctcga
      181 ccgcgatttc gggcggccga cgagaaccag cgaaaccagg atgcacaggc atctccagcc
      241 ccccccagaa cggcaaagcc aagcgaaagg gcgagagtat gtctggcacg gcaagccccg
      301 tcaatgcgcc gcgcgactcc gcaatggcgg ccgacgaccc ggccacgccg ctgcgacgtg
      361 gattccgagc ttgccgtgcc cggcgatctc cggcatcatt ggaccccgcg ccgaagatga
      421 cttgccacct gaccgatcat ttcccatgcc gatgacaagc cgccgccaaa ctccattctc
      481 ctggccaaaa atccgtatcc ctgccgaaca acgtgctgca ggcggtcgcg cggatggtgc
      541 atgggtctga gttcgctcgt ccgcgtcatc cgcgaactcc tgcacgaggc agcgcgcgac
      601 ctggcgccga cgttgatcgt cgtcgtcctc ttccaggtgt ttttcatcca gcgcatgccg
      661 gacagtccgg tggcgctgat cggcggtttt gcgatcgtcc tgctcgggct ggcattgttt
      721 gtcaagggtc tcgacgctgc gatctttccg gccggcgaga cgatggcttt cgacttcgcc
      781 cgccgcggat ctttcctgtg gctgatgctt tttgccttct gcatcagctt cgcctcggtc
      841 gccgccgagc cggcgctgat ggccgtcgcc tacaaggctg aagcggtctc gggcggcagc
      901 atgggcgcgc tgaccctgcg catggtcgcc gcactgggcg tcggcctggc ggtgatgctc
      961 ggtgtctggc gcatcgttct cgggcactcg atcggcaact acctgatccc gggttacctg
     1021 ttggtcgtcg tgttgacggt gttttcgcca ccacaactgg tcggtctggc tttcgactcc
     1081 ggcggcgtcg tcgcctcgac ggtcaccgcg ccgctgttga ccgccctggg ggtcggcctg
     1141 gcgaccagta tccgcggccg caacccgatg ctcgacggct tcggcctgat cgccttcggc
     1201 gccctggcgc cgatgatcat catccagatt tacggcattc tgttcttcgt tcccgccgca
     1261 cagggcaacg cggtgctgat gatgccgagc gacgaccagc accagtacgc ggcgctggag
     1321 ttgctgatcg atttcctgat catggtcagg aacctgttgc cgatcattgc cgtggtcctg
     1381 ttttcgagtt tcgtcatcct gcggcggccg ctcgccgacc ccaagggtct ggcagtgggc
     1441 atggtgctgg tcgccgtcgg cctgtttctg ttcgacgaag ggctgcagat tgggctgttc
     1501 ccgctcggcg acgagatgac caacggcctg atgcgcgacg acgtgccgat gtgggcggtc
     1561 tatctctacg gcttcctgat cggcttcgcc accaccatgg ccgaaccggc gctgatcgcg
     1621 ctgtcgatca aggccgacga ggtcagcctg gggcaactca agggcctctg gttgcgcacg
     1681 ctggtctcga tcggcgtcgg catcggcatc gtcatcggct gtgcacgcat catcgacggc
     1741 atcaacatcg cctactggct gatccccggc tatctgctgg tgctggcgat gactaaattc
     1801 gcgccggact tcatcgttcc gatcgcctac gactgcgggg gggtgaccac ctcgacggtg
     1861 accgtgccgc tggtgaccgc gctcggcgtc ggcctggccg aacgtacccc cggccgtgac
     1921 ccgatgatcg acggtttcgg cctgattgcc tttgcctcgc tgctgccgat gatcatcgtc
     1981 atgtcctatg gcatgctggc cacgtggctg ctgcggtcgc ggacactcaa ggagaaacaa
     2041 cgcccatgaa attttctgct gtggtgctga tcgttccgga agaagacgag aaccgggcgg
     2101 tcgggattct caaggagtcg ggggccaccg gcctcaccat cctcaagggc aagggtctgc
     2161 ggcacaacga acgcaagacc tttctcgg
//
LOCUS       DAOSIT010000414         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43279_length_2184_cov_10.796618, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000414 DAOSIT010000000
VERSION     DAOSIT010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43279_length_2184_cov_10.796618"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1111)
                     /gene="meaB"
                     /locus_tag="PLL14_09390"
     CDS             complement(113..1111)
                     /gene="meaB"
                     /locus_tag="PLL14_09390"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOG04184.1"
                     /translation="MTSASAGSLAAADQHLVDGVLTGRRRALAKTITLVESTHSDHQR
                     RARQVLTALLPHTGRTIRVGISGAPGAGKSTFIESLGMYLIDRGKRVAVLAVDPSSSV
                     SGGSILGDKTRMELLCQRDEAFIRPSPSAGSLGGVADKTREAMLVCEAAGFDVIIVET
                     VGVGQSETTVAGMVDVFVLLQLPNAGDDLQAIKKGIVEIADLIVFNKADIDERAAAFA
                     RGQMKAALTMLRSASPNWRPPVLTLSALAKRGIAEFWAEIERYQSTLTATGEFDEKRR
                     RQAVDWMWSLIDSGLRRYFRAHPAVRSALPELSRDVAEGRSTPGAAASRLLACLKH"
     gene            complement(1141..>2184)
                     /locus_tag="PLL14_09395"
     CDS             complement(1141..>2184)
                     /locus_tag="PLL14_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOG04185.1"
                     /translation="SRIARNTQLVIQEETHITNVIDPWAGSYMMEKLTQDLVDEAWKI
                     IEEVESMGGMTHAVESGWAKMKIEGCAADKQARIDSGKDVIVGVNKYKLAKEDSLDIR
                     DIDNNAVRDSQIARLKSVRANRDTDQVNAALADLTRCAETGEGNLLDFTVRAMRLRAS
                     VGEVSDAMEKIFGRFRATQQTVSGVYGAVVEGIASWENLKAEIVRFNDEEGRRPRIMI
                     AKLGQDGHDRGAKVVATAFADLGFDIDVGPLFQTPEEAARLAIENDVHAVGVSSLAAG
                     HKTLLPALVQALKDQGADDIIVFAGGVIPAQDYDFLFQAGAKAIFGPGTRIEDSAMTV
                     LAEIRKARSQATA"
BASE COUNT          343 a          714 c          703 g          424 t
ORIGIN      
        1 cgcgcttttc tgcaagctgg cgaatgatgt cgtgcatatg ttctactccc taacgttcgc
       61 gtcaatcaga actactcaaa caggtcggag gatctcggtc cgatccctgc aatcagtgct
      121 tcagacaggc cagcagtcgc gacgctgccg caccgggcgt gctgcgccct tcggcgacgt
      181 cgcgcgacag ttccggcagc gccgagcgca cggccggatg ggcacggaaa tagcggcgca
      241 ggccggaatc gatcaggctc cacatccagt ccacggcttg ccgacggcgc ttctcgtcga
      301 attcgccggt ggccgtgagg gtgctctggt agcgttcgat ctcggcccag aattcggcga
      361 taccacgctt ggccagggcg ctgagggtga gtaccggcgg ccgccagttc gggctggcgc
      421 tgcgcagcat ggtcagcgcc gctttcatct ggccgcgcgc gaaagcggcg gcgcgttcgt
      481 cgatgtcggc cttgttgaag acgatcaggt cggcaatttc gacgatcccc ttcttgattg
      541 cctgcaggtc gtcgccggcg ttcggcaact gcagcaacac gaagacgtcg accatgccgg
      601 cgaccgttgt ttccgattgc ccgacaccga ccgtctcgac gatgatcacg tcgaatccgg
      661 ctgcttcgca gaccagcatc gcttcacgcg tcttgtcagc caccccgccc agcgagccgg
      721 ccgaagggct cggccggatg aaggcctcgt cgcgctggca cagcaactcc atgcgcgtct
      781 tgtcgccgag gatcgaacct cctgaaaccg acgacgaggg gtccacggcc agcacggcga
      841 cgcgctttcc cctgtcgatc agatacatcc cgagcgactc gatgaaggtc gatttgccgg
      901 caccgggagc gcccgagatg ccgacccgaa tggtccgccc ggtgtgcggc agcaatgccg
      961 tcaatacctg ccgcgcccgc cgctggtggt cgctgtgcgt cgactcgacg agggtgatgg
     1021 ttttggccag cgcccggcgg cgaccggtca ggacaccgtc cacgaggtgc tggtcggcag
     1081 ccgccaggct gccggcagat gcactggtca tatccatgcg ctaccgtgtg tgctgcgcgt
     1141 tcaggccgtt gcctgtgaac gtgccttgcg gatttccgcc agcacggtca tggccgagtc
     1201 ctcgatgcgt gtgccggggc cgaaaatggc cttggcgccg gcctggaaca ggaagtcata
     1261 gtcctgcgcc ggaatcaccc cgccggcgaa gacgatgatg tcgtcggcgc cctggtcctt
     1321 gagcgcctgc accagcgccg gcaacagcgt cttgtggccg gcggccagcg aggagacgcc
     1381 gaccgcgtgc acgtcgttct cgatggccag gcgagcggcc tcttccgggg tctggaagag
     1441 cggcccgacg tcgatgtcga agccgagatc ggcaaaggcg gtagccacca ctttggcacc
     1501 gcggtcgtgg ccgtcctgac cgagcttggc aatcatgatc cgtggccggc ggccttcctc
     1561 gtcgttgaac ctgacgattt ctgccttgag gttttcccag ctggcgatgc cttccacgac
     1621 cgcgccatag acacccgaaa ccgtttgctg cgtggcgcgg aaacgaccga agatcttctc
     1681 catcgcgtcc gagacttcgc ccaccgaggc gcgcaggcgc atcgccctga ccgtgaagtc
     1741 gagcagattg ccttcgccgg tttcggcgca gcgcgtcagg tcggccagtg ccgcgttgac
     1801 ctggtcggtg tcgcgattgg cgcggacgct cttcaggcgc gcaatctgcg agtcgcggac
     1861 ggcgttgttg tcgatgtcgc gaatgtcgag gctgtcttcc ttggcgagct tgtacttgtt
     1921 gaccccgacg atgacgtcct tgccggaatc gatacgtgcc tgcttgtcgg cggcgcagcc
     1981 ctcgatcttc atcttggccc agccggattc gacggcgtgc gtcatgccgc ccatgctctc
     2041 gacttcctcg atgatcttcc aggcttcgtc caccaggtcc tgcgtcagct tctccatcat
     2101 gtacgagccg gcccaggggt cgatgacgtt ggtgatgtgc gtctcttcct ggatgaccag
     2161 ctgcgtgttg cgggcgatgc gcga
//
LOCUS       DAOSIT010000415         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43371_length_2181_cov_16.384290, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000415 DAOSIT010000000
VERSION     DAOSIT010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43371_length_2181_cov_16.384290"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLL14_09400"
     CDS             complement(<1..206)
                     /locus_tag="PLL14_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04186.1"
                     /translation="MSGDSLREIIDTARQALPEVPADVWDRFEAAVRREHGATRIYIA
                     AHTKRRLLREIAAPAAEEDAAALAL"
     gene            complement(193..>2181)
                     /locus_tag="PLL14_09405"
     CDS             complement(193..>2181)
                     /locus_tag="PLL14_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOG04187.1"
                     /translation="VHYLLSDAVHTLLDDIGRRAEAASSALPEFGARFRRGARPRSLQ
                     TVSQWADKQRWLIAGTNAPGKWRTDLTPYLRDIQDDLSEHSPVRTVVFIKSSGVGGTE
                     AMFNWLGYCMHHLGNRDMLVVVPSLELRDRSFNPRLSKMIGENPPLADLVSRASRSSA
                     NRADILEYGANARIIKAGANSADSLRSDHLPYVICDEVDAYKWDVGGEGDPMTLIENR
                     QRTFSRAKTFLVSTPTNADESRIDQAYQRSDRRRYHVPCPHCGDFHHLKFSNLKYRTE
                     VAESPTPGAAEAKVVVDAWYVCESCGAEILEGEKPTLLASGRWIAERPRVKLVRGYHI
                     NSLYAPIGLGLGWRQIAQKWVDVQGDTAALKAFVNTYLGEVWREEGDGADAASVLARV
                     EPYTLETLRAARKVRRLTAGVDVQKDRLECSLVAWGNGEEGWLLDHEIFPGDTALPGP
                     WEDLDEYLRDARVTMACVDSGYNTSMAKAFCAGKIWALPTKGIAGMGRPLIEDERLRK
                     RRLRVRRKKGQPIEPLGVDQAKSLIYARLKLPTPGPGYLHFPADPAFDDEYFAQLAAE
                     QLVKRIRGSRVFSEWKQIRPRNEALDCLILALAACRLAGPLADVRISSAGAQPAPGAA
                     GAEIAAAESASSATPADTAAQVFAAMMAARAAKSRVRR"
BASE COUNT          327 a          689 c          775 g          390 t
ORIGIN      
        1 agggccaggg cggcggcgtc ttcttcagcc gccggcgcgg cgatctcgcg cagcagtcgg
       61 cgcttggtgt gggcggcgat gtagattcgc gtcgccccgt gctctcgtcg cacggcggcc
      121 tcgaagcggt cccatacgtc ggcaggcacc tccggcagcg cttgcctggc ggtgtcgatg
      181 atttcgcgca ggctatcgcc ggacacggga ctttgcggcg cgggcggcca tcatcgcggc
      241 gaacacttgc gcggcggtgt cggctggggt tgctgatgat gcggactcgg cggcggcgat
      301 ctctgcgcct gcggcgccag gcgcgggctg cgcgccggcg gacgatatcc gcacgtcggc
      361 cagcggcccg gcaagccgac aagcggcgag cgcgagaatc aggcagtcaa gcgcttcgtt
      421 ccgtgggcga atctgcttcc actcgctgaa cacgcgcgag ccgcgaatgc gcttgaccag
      481 ctgctcggcg gcgagctgcg cgaaatactc gtcgtcgaag gctgggtcgg ccggaaagtg
      541 caggtagcca gggccaggcg tcggcagctt gagtcgggca tagatcagcg acttggcttg
      601 atcgacgccg agcggctcga ttggctgccc cttcttgcgg cgcacgcgca ggcgcctttt
      661 gcgtaggcgc tcgtcctcga tcaatgggcg gcccatgccg gcgattcctt ttgtcggcaa
      721 ggcccagatt ttcccggcgc agaacgcttt cgccatggat gtgttgtatc cagagtcaac
      781 gcatgccatg gtgacgcgcg cgtcccgcag gtactcatca aggtcttccc atggcccagg
      841 aagcgccgtg tctccgggga atatctcatg atcgagtagc cagccctcct cgccgttgcc
      901 ccaggcaacg agcgagcact ccaggcgatc tttctggacg tcaacgccgg cggtgagccg
      961 gcgcaccttg cgggcggcgc gcagcgtttc cagggtgtag ggctcgacgc gtgcgagcac
     1021 gctggccgcg tcggcgccat cgccttcttc gcgccagact tcgccgaggt aggtattgac
     1081 gaaagccttc agcgcggcgg tgtcgccctg tacgtccacc cacttctgcg cgatctggcg
     1141 ccagcccagc ccgaggccta tcggggcata gagcgagttg atgtgatagc cgcgcaccag
     1201 cttgacgcgc gggcgctcgg cgatccaccg gccgctcgcc agcagggtcg gcttctcgcc
     1261 ttcgaggatc tcggcgccgc aggattcgca gacgtaccag gcgtcgacga cgaccttcgc
     1321 ttcagcggct cccggcgtgg gcgactcggc cacctctgtg cggtacttga gattgctgaa
     1381 tttgaggtga tggaagtcgc cgcagtgcgg gcaagggacg tgatagcggc ggcgatcgct
     1441 gcgctgatac gcctgatcga tgcggctctc gtcggcgttt gttggcgtcg agacgagaaa
     1501 ggtcttcgcg cgcgagaatg tgcgctgccg gttctcgatc agggtcatgg ggtcaccctc
     1561 cccgccgacg tcccacttgt aggcgtccac ctcgtcgcag atcacgtagg gcaagtggtc
     1621 ggagcgcagc gagtcggcgc tgttggctcc cgccttgatg atcctggcgt tggcgccgta
     1681 ctcgaggatg tcggcccggt tggcgctgct gcgcgaggcc cggctgacca ggtcggcgag
     1741 cggcggattc tcgccgatca tcttggacag gcgcggattg aatgagcggt cgcgcagttc
     1801 gagcgacggc acgacgacaa gcatgtcgcg gttgcccagg tggtgcatgc agtagccgag
     1861 ccaattgaac atggcctccg tgccgccgac accggagctc ttgatgaaaa cgacggtgcg
     1921 cacaggggag tgctcggaca gatcgtcctg aatgtcgcgc aggtaggggg tgaggtcggt
     1981 ccgccatttt ccgggcgcat tggtgccggc gatcagccag cgctgcttgt ccgcccactg
     2041 ggagacggtc tgcagcgagc gcggccgggc gccacggcga aagcgcgcgc cgaactcggg
     2101 tagcgcgctg ctcgccgctt cggcgcgccg gccgatgtcg tcgagcaggg tatgcacggc
     2161 gtcggaaagc aggtagtgca c
//
LOCUS       DAOSIT010000416         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43558_length_2174_cov_7.008023, whole genome shotgun sequence.
ACCESSION   DAOSIT010000416 DAOSIT010000000
VERSION     DAOSIT010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43558_length_2174_cov_7.008023"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..>2174)
                     /locus_tag="PLL14_09410"
     CDS             complement(345..>2174)
                     /locus_tag="PLL14_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOG04188.1"
                     /translation="FGAMDEELVVKAIAGSLRSQCDEGLFAIGRRLAFLSGEPEGSLP
                     IEHLIAESLGGAFEDAGLVGKAKLEILQLMERHAVDVFGPTIDDLNAFLIGRGVLPQL
                     SRTCSPSPNHPRNATDPGKPAESADLFALLQELVRPSQVSAGTGGAGAGSGAGCGPST
                     GVSMGGASPVGMAVAMEQVMASLDTLQRAVPVQTAAALMDNVLRQFHSSDAGQNLDYL
                     HSVTVDIVATLFDFIFDDASVADPIKALVARMQIPVLKVAMLDKTFFSSKSHPARRLL
                     DGISRAAVRCGPQAGHDNPLYARVAEIVERLQNEFSQDTELFDTLCVELDAFLESQEA
                     AADAHAVNAAPLVAEQERYELAAAAADHALVGWLAMPLPSAVTDLLDNEWRKLLVRHS
                     MDGDNAAWEAAMDTVSELVASVDIPSDVPSRKRLAARLPTLVRRICNGLGRLEMADDR
                     RLALIDALFSVHAAVLRGTAPPIVTPRPVLPAAEPVPVSLAREQVVRGETQIERISLA
                     GENELPPSRDRADALSLVGSLQRGDWVEFANPESGPLRYRLSWISPQRGILLFTTSQS
                     PRAISVTPNALALQIERGEAILMPPEPMFDRAVNRALQKLKAA"
BASE COUNT          423 a          695 c          675 g          381 t
ORIGIN      
        1 cgtcgagacg gccggcgagc acatcgtagg ctccgagcgc tgggcgcgcc ggtagtcaga
       61 cagtgtatat cagttgcagc agccggtggc cgtccagttg cgcaggctgc cgcggacgta
      121 cggcacgggt tgctcgcagg caatgccgaa ctgtagacgt cctgtctggt cgctctgcca
      181 ggcgacggcc ccagggcgat cgtcgtcgct ggtagtttca atttcgaacc ggagcgcatg
      241 gtgaagaatc aggcgcgcca attcggatgg cgacatcgtt gcgtcgtatt cctcatggcg
      301 gcatctggct caagtcacct tatgaactcc cggaaccagc agagttacgc agccttcagt
      361 ttttgcagtg cgcggttaac ggcgcgatcg aacatcggtt ccggcggcat gaggatcgct
      421 tcaccgcgct cgatctggag cgccagggca ttcggggtca ccgatatcgc gcgcggcgac
      481 tgcgaggtag tgaatagcag aatgcctcgc tgcgggctaa tccacgataa gcgataacgc
      541 aagggtcctg attctggatt cgcaaactct acccaatcgc cacgctgcag gcttcccacc
      601 agcgacaggg cgtctgccct gtctcgcgaa gggggcagtt cattctctcc cgccaacgaa
      661 attcgttcga tctgcgtttc gccgcgcacg acctgctcgc gagcaagact gaccggcacg
      721 ggctccgccg ccggcaacac cggccgtggc gtgacgatcg gcggtgccgt accccgcagg
      781 actgcggcat gcacactgaa gagcgcatcg atcagggcca ggcggcgatc atctgccatc
      841 tccaggcggc caagcccgtt gcagatgcgg cgaaccaggg tcggcaagcg ggccgccagc
      901 cgcttccggc ttggcacatc cgacggaatg tcgacgctcg ccaccagttc actgaccgtg
      961 tccatggctg cctcccaggc ggcattgtca ccgtccatag agtgccgaac gaggagtttg
     1021 cgccattcgt tgtccagcag atcagtcacc gccgatggca gtggcatcgc cagccagccg
     1081 acgagggcgt gatcggctgc cgcagccgcg agttcgtagc gctcctgttc ggcaaccagt
     1141 ggcgccgcgt tgacggcgtg ggcatcggct gcagcctcct ggctttcaag gaaagcgtcc
     1201 agttcgacgc acagcgtatc gaacaattcg gtatcctgac tgaactcgtt ctgcagccgt
     1261 tcgacgattt ccgcgacccg cgcgtacagt gggttatcgt gacccgcttg cgggccgcaa
     1321 cgcaccgccg ctcgcgaaat cccgtccagc aggcgccgcg ccggatgcga cttgctcgaa
     1381 aagaaagtct tgtccaacat ggcgaccttc agcaccggaa tctgcatgcg cgcaaccagg
     1441 gccttgatcg gatcggcgac agaagcgtcg tcgaagatga agtcaaacag ggtggcaacg
     1501 atatccacgg tgaccgaatg cagatagtcg aggttctggc ccgcgtcact gctatggaac
     1561 tgccgcaaga cgttgtccat caatgccgca gccgtttgca ccggcaccgc cctctgcaga
     1621 gtgtccagtg aggccatgac ctgctccatg gcaacggcca tgccgacggg cgacgcaccg
     1681 cccatggaca cgccggtgga agggccgcag cctgcgccag agccggcgcc tgcgccaccc
     1741 gtcccagcgg acacttgcga cgggcgcacg agttcctgca gcaaggcaaa gagatcagca
     1801 gattcggcag gcttgccggg gtcagtcgcg ttgcgcgggt ggtttggtga cggggaacag
     1861 gttcgggaga gctgcggcag cacaccacgg ccaatcaaga aagcattcag atcgtcaatg
     1921 gtcgggccaa agacatcgac ggcgtggcgc tccatgagtt gcaggatttc cagcttcgcc
     1981 ttgccgacca acccggcatc ctcgaatgca ccgcccagcg attcggcgat gagatgttct
     2041 atgggcaggc tgccttccgg ttctccagac aagaacgcca gccggcgccc aatggcaaac
     2101 agcccctcgt cacactgact acgaaggctg ccagcgatgg ccttgacgac cagttcctcg
     2161 tccatggcgc caaa
//
LOCUS       DAOSIT010000417         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43576_length_2173_cov_9.244570, whole genome shotgun sequence.
ACCESSION   DAOSIT010000417 DAOSIT010000000
VERSION     DAOSIT010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43576_length_2173_cov_9.244570"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="PLL14_09415"
     CDS             complement(<1..1810)
                     /locus_tag="PLL14_09415"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HOG04189.1"
                     /translation="MSADFIDLPQLLSARGTVRLPGSKSISNRVLLLAALAAGETEVR
                     DLLLSDDTARMLDALRTLGVGVEPGAGNDFRISGVAGRFPVRTSELFLGNAGTAFRPL
                     TAVLALAGGHYRLAGVARMHERPIGDLVDALRQLGADISYLGSEGFPPLAIRPPALLP
                     GRVVSLRGDVSSQFLTGLLMALPLLGVETTVEVIGELISKPYVEITLASMARFGVQVR
                     RDGWRRFTVLADSGYRSPGVVHVEGDASSASYFLALGAIGGGPLRVEGVGLDSIQGDV
                     RFTEALAAMGAQVSSGDNWIEASAPVAGTGTLRGISLDCNHIPDAAMTLATTALFAVG
                     PTTLRNIASWRVKETDRIAAMATELRKLGVRVEAGDDFIRVHPPGAGQFLHPAAIDTY
                     DDHRIAMCFSLAALGTPLRINDPQSVGKTFPDYFARFAEVSRGVPVIAIDGTSASGKG
                     TIATRVAQALGWHYLDSGALYRLTALAAGRAGIGVDDEAGVAAIAADLDVVFAGASIT
                     LSGEEVSNAIRSEEISAAASRVAALPAVRAALLFRQRAFRRPPGLVADGRDMASVVFP
                     DALTKIFLTASVEMRAWRRHKQLIEKGMAASIAALSL"
     gene            complement(1807..>2173)
                     /locus_tag="PLL14_09420"
     CDS             complement(1807..>2173)
                     /locus_tag="PLL14_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOG04190.1"
                     /translation="VIRELSAAEHDQVFAAVSHLPHLLAFALVDELARRDNRDLLFAF
                     AASGFRDFTRIAASHPEMWRDICLANRTALLTELDRYRTQLDELRDALQRADGASLEA
                     TFNVARTARRAWAESRAGE"
BASE COUNT          392 a          741 c          724 g          316 t
ORIGIN      
        1 ccagcgagag ggctgcaata ctagcagcca ttcctttttc gatcaactgc ttatgacgcc
       61 tccaggcacg catctcgacg ctggcggtga ggaagatctt ggtcagggcg tcgggaaaca
      121 ccaccgaggc catgtcgcga ccatcggcga ccagtccggg tggccgccgg aaagcccgct
      181 gccggaacag gagcgcggcc cgcacggcag gaagcgcggc cactcgcgag gcggcggcgg
      241 aaatttcctc gctacggatg gcgttggaaa cctcttcacc ggacagcgtg atcgacgccc
      301 cggcgaatac gacatccagg tcagcggcga tggccgccac cccggcttcg tcatcgacgc
      361 cgatccctgc ccgtccggca gccagcgcgg tcaggcggta caaggcgccg gaatcgaggt
      421 agtgccagcc cagagcctgg gcgacgcgtg tggcaatcgt tccctttccg gaagccgagg
      481 tgccatcgat ggcaatcacc ggcacgcccc ggctgacctc ggcaaagcgc gcgaagtagt
      541 cgggaaaggt cttgcccacg ctctgcgggt cgttgatgcg caagggcgtg ccgagcgccg
      601 ccagcgagaa gcacatggcg atgcggtggt cgtcgtaggt gtcgatcgcg gccggatgca
      661 ggaactgccc ggcgcctggc gggtggacgc gaatgaaatc gtcaccggcc tcgacacgca
      721 cgccgagctt gcgcaactcg gtggccatcg cggcgatgcg gtcggtttcc ttgacgcgcc
      781 aactggcaat gttgcgcaag gtggtcggcc ccaccgcaaa cagggcggtg gtcgccagcg
      841 tcatggccgc gtccgggatg tgattgcaat cgaggctgat gccgcgcagc gttcccgttc
      901 cggcgaccgg cgccgaggcc tcgatccagt tgtcgcccga cgagacttgc gcgcccatcg
      961 ctgccaatgc ctcggtaaaa cgcacatcgc cctggatcga gtccagacca accccctcga
     1021 cacgcagcgg cccgccgcca atcgcgccaa gggccagaaa atacgacgcc gacgaggcat
     1081 cgccctcgac atgcaccacc ccaggcgagc gatagccgct gtccgccagt acggtgaagc
     1141 gccgccagcc gtcgcgccgc acctgaacgc cgaaacgagc catgctcgcc agtgtgatct
     1201 cgacgtaagg tttggagatc agttccccga tcacctccac ggtggtttcg acgccgagca
     1261 gcggcagcgc catcagcagg ccggtcagga actgactcga tacgtcaccg cgcagactga
     1321 ccacgcgtcc cggcagcagc gcgggtggac gaatggccag ggggggaaag ccttcgctcc
     1381 ccaggtagct gatgtcggca ccgagttgac gcaacgcatc gacgaggtcg ccgatcggtc
     1441 gttcgtgcat gcgtgcgacg ccggccagcc ggtaatgccc gccggccagg gccagcaccg
     1501 cggtcagcgg ccggaaggcg gtacctgcgt tgcccagaaa gagctcgctc gtgcggaccg
     1561 ggaagcgccc ggcgacaccg ctgatgcgaa agtcgttgcc ggcccccggc tcgacaccga
     1621 cgccgagggt cctgagcgca tcgagcatgc gcgccgtatc gtcggacagc agcaggtcgc
     1681 gcacctcggt ctcgcccgcc gccagcgcgg ccagcagcag gacccgattg gagatactct
     1741 tcgaacccgg caggcgaacc gtcccgcgtg ccgaaagcag ttgcggcagg tcgatgaagt
     1801 ccgcgctcat tcgcctgccc tgctctcggc ccacgcacgc cgcgcggtcc tggcgacatt
     1861 gaaggttgcc tccaggctgg ccccgtcggc acgctgcagg gcatcgcgca actcgtcgag
     1921 ctgcgtccgg tagcggtcga gttcggtgag cagtgccgtc cggttggcca ggcagatgtc
     1981 gcgccacatc tccggatggc tggcggcgat gcgcgtgaag tcgcgaaagc cgctggccgc
     2041 aaaagcgaag agcaggtcac gattgtcgcg ccgcgccagt tcatctacca gtgcgaaggc
     2101 cagcaggtgc ggcaggtggc tgaccgccgc aaagacctga tcgtgctcgg ccgcgctcaa
     2161 ctcccggatc act
//
LOCUS       DAOSIT010000418         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43655_length_2170_cov_8.583924, whole genome shotgun sequence.
ACCESSION   DAOSIT010000418 DAOSIT010000000
VERSION     DAOSIT010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43655_length_2170_cov_8.583924"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLL14_09425"
     CDS             <1..594
                     /locus_tag="PLL14_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOG04191.1"
                     /translation="SLRELCARMDEPLRSALLSLPALYGGVETLAAATKVLPAWPQVT
                     AALETLRQLQASLPDLPLSFDLADLRGYHYHNGVVFAAYCTDFSSAVARGGRYDGVGR
                     AFGRDRPATGFSMDLRDLAGLAASGRAPGAILAPCSANSAPLADTIAVLRARGEVVVE
                     LLPSETRSEGPRCDRQLLERDGQWLIEAITGTDHRNG"
     gene            587..1894
                     /locus_tag="PLL14_09430"
     CDS             587..1894
                     /locus_tag="PLL14_09430"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOG04192.1"
                     /translation="MARNVVVVGTQWGDEGKGKIVDWLTDHARGVVRFQGGHNAGHTL
                     VVGDKVYKLNLVPSGIVREGVACFIGNGVVLDIHHLISEIRVLESGGIDVRSRLRISP
                     GCPIILPYHAALDLAREAAKCSDLRIGTTGKGIGPAYEDKVARRALRVYDLFHPTRFA
                     DKLKENLDYHNFVLTRYLGAEAVDFDAVLAQAMADAEEIKPLVADVSAALHAINQAGH
                     SLLFEGAQGALLDIDHGTYPFVTSSNCVAGQAAAGSGIGPGMLHYVLGITKAYCTRVG
                     GGPFPSELDIETAGLPGYQMSQKGREFGTVTGRKRRCGWLDAAALKRSIQINGVTGLC
                     ITKLDVLDGLPELKICRAYQLDGKTIDLLPMGADEVAACQPILETWPGWDESTVGVST
                     MQGLPAAARAYLERIEELCGIPVDIVSTGPERGETVVRRHPLG"
BASE COUNT          381 a          615 c          728 g          446 t
ORIGIN      
        1 agtctgcgcg agctttgcgc caggatggac gagcctctgc gctcggcgct gctgagcttg
       61 cccgcgctct acggaggcgt cgaaacactt gcggcggcga cgaaggtctt gcctgcctgg
      121 ccgcaggtca cggccgcttt ggagaccttg cggcagttgc aggcgtcgtt gcccgatctg
      181 ccgctatcgt tcgacctcgc tgacctgcgc ggttaccatt atcacaacgg tgtcgttttt
      241 gcggcttact gcactgattt ttcgagtgcc gttgcacgtg gcgggcgtta tgacggcgtt
      301 ggcagagcct tcggtcgcga ccgtccagcg acgggcttct cgatggacct gcgggatctg
      361 gcagggcttg cggccagtgg cagggcgcca ggggcgattc tggcgccctg ttcggcaaac
      421 agcgcccctc tggcagacac gattgcggta ttgcgtgcgc ggggcgaggt cgtcgttgaa
      481 ctcctgccgt ccgaaacacg tagcgaaggg ccgcggtgtg accgccaatt gcttgaacgt
      541 gacgggcaat ggctaattga ggcaatcaca ggaacggatc acaggaatgg ctaggaacgt
      601 cgtggtggtg ggcacccaat ggggcgatga gggtaagggc aagatcgtag actggctgac
      661 cgatcatgcc aggggtgtcg ttcgttttca gggcggtcac aacgccggtc acacgctggt
      721 ggtcggcgac aaggtgtaca aactcaatct ggttccctcc gggatcgttc gtgagggggt
      781 tgcctgcttc atcggcaacg gggtcgtgct ggatattcat cacttgatca gtgagatcag
      841 ggtcctcgag tcgggtggca tcgacgtccg ctcgcgcctt cggatcagtc cgggttgtcc
      901 gatcatactg ccctaccacg ctgctctcga cctcgcccgt gaggcagcga agtgctcgga
      961 tttgcgcatc ggcaccaccg gcaagggcat tggcccagcc tatgaagaca aggtggcgag
     1021 gcgggcgctg cgcgtctatg atctgttcca tcccactcgt tttgccgaca agctgaagga
     1081 aaacctcgac taccacaact tcgtgctgac ccgctatctg ggcgccgaag cggtcgattt
     1141 cgacgctgtc ctggcccagg cgatggccga tgccgaagag atcaagcccc tggtggccga
     1201 tgtttcggcg gcattgcatg cgatcaatca ggccggacac agtctgctgt tcgagggagc
     1261 acagggtgcg ctgctcgaca tcgatcacgg cacttaccct tttgtcacct cctcgaactg
     1321 tgtcgcgggt caggccgcag ccggatcggg catcggtccg gggatgctgc attacgtgct
     1381 gggaatcacc aaggcgtact gtacgcgcgt cggtggcgga cctttcccga gtgaactcga
     1441 tatcgagacg gcaggactgc cgggctatca gatgtcgcaa aaggggcgtg aattcggtac
     1501 cgttaccggg cgcaagcgtc gctgcggctg gctcgatgcg gcagcgctca aacgctcgat
     1561 ccagatcaac ggtgtcaccg gtctgtgcat caccaaactc gacgttctcg atggattgcc
     1621 ggagctgaag atctgccgcg cctatcagct tgatggcaag acaatcgacc tgctgccgat
     1681 gggggccgac gaggttgccg cttgccagcc gatactggaa acctggccgg gttgggacga
     1741 gtctacggtc ggtgtcagta cgatgcaggg tttgccggca gcggcgcgtg cttatctgga
     1801 gcgtatcgag gaactgtgcg ggattccggt cgacatcgtc tcgaccggac ccgagcgcgg
     1861 agagacggtg gtccgtcgcc acccattggg gtgatcggga aagttccagg tcacggcact
     1921 gcccgccacg gccagctggc cgccagtaac cgtcgggacg gctgcgagac gacgttcagg
     1981 gtcttcggag ggtactgaac tttcgcgtgg tgcaccatcg gggttgtcgc gaagggtgct
     2041 gtgcgggctt taacgtggga ggtggacaca tgggcattct tgactggttc aagaaccgtc
     2101 cggctcagtt tgatccggat cgggtttcgg acgaaatgat tcgcgcggct gcagacaagg
     2161 cgatcaccct
//
LOCUS       DAOSIT010000419         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_43942_length_2160_cov_8.914964, whole genome shotgun sequence.
ACCESSION   DAOSIT010000419 DAOSIT010000000
VERSION     DAOSIT010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43942_length_2160_cov_8.914964"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLL14_09435"
     CDS             <1..1002
                     /locus_tag="PLL14_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOG04193.1"
                     /translation="RRLEFEPFRLRVTGTAGSGKTQLALAEFRATVEAGKRPLYVCFN
                     RPLADHFQRIAPAGGVACTYHQLCDQRLRQAGETPDFTLPDAFERLQQRAAELPVSDA
                     SLFDTLIVDEGQDISEPWRDFIFRHARADARILWLEDPLQNLYGRPPTPLPGWVTLRT
                     QSNYRSPRPIVELLRRLLPGELDIEAASPIAADDIDILDYADTQGMFARVKEAIRLCY
                     SAGFKKHDLAIVSYHGRAQSHLLALDALGQTSLRHFTGAYDLFGQPVYSQGDVLVESV
                     YRFKGQAAPAVIFTEIDFTVLDDQAVRKLFVGATRSSMKLVLILHQKSAAGLLERLH"
     gene            1100..>2160
                     /locus_tag="PLL14_09440"
     CDS             1100..>2160
                     /locus_tag="PLL14_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG04194.1"
                     /translation="MPNTLPRILIQPLKPAVIAGIAQKLPVLIRVQAPAAAPTESKAR
                     KPYHLALVIDRSGSMSGAPLIEAIRCTRHIIEQLEPTDMASLVIFDDRVQTLVAAQPV
                     GDRQALHLALSRVHGGGSTNLYGGWKAGADGLLPAAGQAALTRVILLSDGNANVGEIT
                     DATAMATLCAHAAEHGVTTSTYGLGSHFNEDLMVEMAKRGGGNHYYGDSASDLFEPFS
                     AEFDFISALCARQVRLSLAAAPQVRIRLLNDYPLRSDTTFPVVHLPDIAFGAEAWALV
                     ELELPLALAADGAGPLLQVAVNASTPEGEPATFADATLTLPAVSMAVWETVPEDALVV
                     TRQSELAAGKLLEQARAAA"
BASE COUNT          350 a          808 c          653 g          349 t
ORIGIN      
        1 cggcggctcg aattcgagcc cttccgcctg cgcgtgaccg gcaccgccgg atcgggcaag
       61 acacaactcg ccctggccga attccgcgcc accgtcgagg ccggcaagcg gccgctgtat
      121 gtctgcttca accgcccgct ggccgaccac ttccagcgca tcgcaccggc cggcggcgtg
      181 gcctgcacct atcaccagct ctgtgaccag cgcctgcgcc aggccggcga gacacccgac
      241 ttcaccttgc ccgacgcctt cgagcgcctg cagcaacgcg ccgccgaact gccggtcagc
      301 gacgcctcgc tgttcgacac cctgatcgtc gacgaaggcc aggacatctc cgagccctgg
      361 cgcgacttca tcttccggca tgcgcgcgcc gacgcgcgca tcctgtggct cgaagacccg
      421 ctgcagaatc tctacggtcg cccgccgaca ccgctgcccg gctgggtgac gctgcgcacg
      481 cagagcaact accgcagccc gcgaccgatc gtcgaactgc tgcgccgcct gttgcccggt
      541 gagctcgaca tcgaggcggc ctcgccgatc gccgccgatg acatcgatat cctcgactat
      601 gccgacaccc agggcatgtt cgcccgcgtc aaggaagcca tccggctctg ctactccgcc
      661 ggcttcaaga aacacgacct ggccatcgtc agctaccacg gccgcgcgca gtcgcatttg
      721 ctggcgcttg acgccctcgg ccagaccagc ctgcgccact tcaccggcgc gtacgacctg
      781 ttcggacaac cggtctatag ccagggcgat gtgctggtcg aatcggtgta ccgcttcaag
      841 ggccaggccg cgccggcggt cattttcacc gagatcgatt tcacggtcct cgacgaccag
      901 gcggtgcgca agctcttcgt cggcgccacg cgcagttcga tgaagctggt gctgatcctg
      961 caccagaagt ccgccgccgg attgctcgaa cgtctgcact gaatcatcac cgctgcgccc
     1021 ggtggcgcat cctgtgagct cgacggtcgc tatcctgcag acatctgccg gcaatggtgc
     1081 gggcggaaca ggagacccga tgcccaatac cctaccccgt atcctcatcc agccgctcaa
     1141 gccggcagtc atcgccggca tcgcccagaa gctgccggtc ttgatccgtg tgcaggcgcc
     1201 tgctgccgcg ccgacggaga gcaaagcccg caagccctat cacctggccc tggtcatcga
     1261 ccgttccggg tcgatgtcgg gcgcgccgct gatcgaagcc atccgctgca cccggcacat
     1321 catcgagcag cttgagccca ccgacatggc ctcgctggtc atcttcgacg accgcgtgca
     1381 gaccctcgtt gcggcccagc cggtcggcga ccgccaggcg ctgcacctgg ccctgtcgcg
     1441 ggtgcatggc ggcggctcga ccaacctcta cggcggctgg aaggcgggcg ccgatggcct
     1501 gctgccggcc gccggccagg cagcgctgac gcgcgtcatc ctgctctccg acggcaatgc
     1561 caacgtcggc gaaatcaccg acgccacggc gatggccacc ctctgcgcac acgctgccga
     1621 gcatggcgtc accacctcga cctacggcct cggatcgcac ttcaacgagg acctgatggt
     1681 cgagatggcc aaacgcggtg gcggcaacca ttactacggg gatagcgcaa gcgacctctt
     1741 cgagccattc tccgcggagt tcgatttcat ctcggccctc tgcgcgcgtc aggttcgcct
     1801 gtcgctcgcg gcagcgccgc aggtccgcat ccgcctgctc aacgattatc cgctgcgcag
     1861 cgatacgact tttccggtcg tccatctgcc ggacatcgcc ttcggggcgg aagcctgggc
     1921 gctggtcgaa ctggagctgc cgctcgcgct cgccgccgat ggcgccggac cgctgctgca
     1981 ggtagctgtc aacgcctcga ccccggaagg cgagccggcc acctttgccg atgcgacact
     2041 gacgctaccc gccgtatcga tggcggtgtg ggaaaccgtg cccgaagatg cgctggtggt
     2101 cacccgccag tccgaactgg ccgccggcaa gctgctcgaa caggcgcgcg ccgctgccga
//
LOCUS       DAOSIT010000420         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44052_length_2156_cov_7.973822, whole genome shotgun sequence.
ACCESSION   DAOSIT010000420 DAOSIT010000000
VERSION     DAOSIT010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44052_length_2156_cov_7.973822"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLL14_09445"
     CDS             <1..464
                     /locus_tag="PLL14_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG04195.1"
                     /translation="KIIFAVPGMILATLFITFPFIARELIPLMQSQGKDEEEAAISLG
                     ASGWQMFWRVTLPNIKWGLLYGVILSNARAMGEFGAVSVVSGHIRGETNTLPLHVEIL
                     YNEYNAIGAFAAASVLALLALVTLVAKTIVEWRMKAETDMLNAELPGEKTS"
     gene            461..1528
                     /locus_tag="PLL14_09450"
     CDS             461..1528
                     /locus_tag="PLL14_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOG04196.1"
                     /translation="MSIEIRNISKRFGNFVALDNINLDIPTGELVALLGPSGCGKTTL
                     LRIIAGMETADQGEILFSGAEATHLHARERNVGFVFQHYALFRHMTVFENVAFGLRVK
                     PRKERPSDAEIKRRVMELLGLVQLDWLADRYPSQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDTKVRKELRRWLRRLHDDMHISSVFVTHDQEEALEVADRVVVMNHGKIEQI
                     GSPDEVYSSPASPFVYQFLGNVNVFHSRVQGGYAEVECREKPERATAFVRPHDIDITH
                     SIAEGSLEATVQHVHPIGPVVRIELLHGRVIVEVELSRERHEALQLVVGQQVWFRPRQ
                     LRVFGADNQPAAASEKQAFLF"
     gene            complement(1640..1874)
                     /locus_tag="PLL14_09455"
                     /pseudo
     CDS             complement(1640..1874)
                     /locus_tag="PLL14_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611695.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2020..>2156)
                     /locus_tag="PLL14_09460"
     CDS             complement(2020..>2156)
                     /locus_tag="PLL14_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG04197.1"
                     /translation="VFGIIKSVMGFRQFSLRGLKKVTGEWTLVCLAWNLKRMAKLRPQ
                     "
BASE COUNT          404 a          687 c          642 g          423 t
ORIGIN      
        1 tcaagatcat cttcgccgtg ccgggcatga tcctcgccac cctgttcatc accttcccct
       61 tcatcgcccg cgagctgatc ccgctgatgc agtcacaggg caaggacgag gaagaggcgg
      121 caatctcgct cggcgcctcc ggctggcaga tgttctggcg cgtcaccctg cccaatatca
      181 agtggggcct gctctacggc gtgatcctgt cgaacgcccg ggcgatgggc gaattcggcg
      241 cggtgtcggt ggtttccggc cacatccgcg gcgagaccaa cacgctgccg ctgcacgtcg
      301 agatcctcta caacgaatac aacgcgatcg gcgccttcgc cgccgcctcc gtgctggccc
      361 tgctggcact ggtgacgctg gtggccaaga ccatcgtcga atggcgcatg aaggccgaga
      421 ccgacatgct gaacgctgaa ctccccggcg agaaaacttc atgagcatcg aaatccgcaa
      481 catctccaag cgcttcggca atttcgtcgc gctcgacaac atcaacctcg atatcccgac
      541 cggagagctg gtcgcgctgc tcggcccctc cggttgcggc aagacgacct tgctgcgcat
      601 catcgccggc atggaaaccg ccgatcaggg cgagatcctg ttctccggcg ccgaagcgac
      661 ccatctgcac gcccgcgagc gcaatgtcgg tttcgtcttc cagcattacg cgctgttccg
      721 ccacatgacg gtattcgaga atgtcgcctt cggcctgcgc gtcaagccgc gcaaggagcg
      781 cccgtccgat gccgagatca agcgccgggt catggaactg ctcggcctgg tccagctcga
      841 ctggctggcg gatcgttatc cgtcgcaact ctccggtggc caacggcagc ggattgcact
      901 ggcccgcgcc ctcgccgtcg agcccaaggt gctgctgctc gacgagccct tcggcgcgct
      961 cgacaccaag gtacgcaagg aactgcgccg ttggctgcgc cgcctgcacg acgacatgca
     1021 catctcgtcg gtgttcgtca cccacgacca ggaagaagcg ctggaagtcg ccgatcgcgt
     1081 ggtggtcatg aaccacggca agatcgagca gatcggctcg ccggacgagg tgtattccag
     1141 ccccgcctcg cccttcgtgt accagttcct cggcaacgtg aacgtcttcc atagccgggt
     1201 gcagggaggt tacgccgaag tcgagtgccg cgagaagccg gagcgggcca ccgccttcgt
     1261 ccggccgcac gatatcgaca tcacccactc gattgccgaa ggcagcctag aggcgactgt
     1321 gcagcatgtg catccgatcg gaccggtggt ccgtatcgag ttactgcatg ggcgggtaat
     1381 cgtcgaagtc gaactgtcgc gcgagcgcca cgaggcgctg caactggtgg tcggccagca
     1441 ggtctggttc cggccgcgtc agctccgcgt cttcggggcc gacaatcagc ccgcggctgc
     1501 gtcggaaaaa caggctttcc tgttctgatc cggggcaaat cgggcgaacg ggggcgggtg
     1561 atataatcgg cccccacttc gtctgcacaa ccgtacccgg tgatattgag gtcggttgca
     1621 aatcgtagag tccgacgggt taagcggcca tctgcattgc atagtattgc tgactgaaag
     1681 cggtgggcga cagatggccg agtcgagcct gctctcggat gcggttgtag aagatctcga
     1741 tgtattccgt gatctcgcgt ttggcctgct cgcgggtcgc gaagcgacag tgatgcacca
     1801 gttcagtctt cagtgaatcc cagcagcttt tccatcggtg cgttgtccca acagttgccc
     1861 ttgcgaccga ggctgtgtga aaaccccttg gctgtgtact ccaaactagc agcctgtcgg
     1921 acttgaggtt tagaaaaccg ccaaatttca caatgcggaa tgaaaaggcc ggattttctc
     1981 gactttgcgg ccttttggac ggttttctcc tcgttttccc tactgtgggc gcaatttggc
     2041 catgcgcttc aagttccacg ccaggcagac cagcgtccac tcgcccgtga ccttcttcaa
     2101 accgcgcagc gagaactggc gaaagcccat gaccgatttg atgatgccga agaccg
//
LOCUS       DAOSIT010000421         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44243_length_2149_cov_9.993314, whole genome shotgun sequence.
ACCESSION   DAOSIT010000421 DAOSIT010000000
VERSION     DAOSIT010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44243_length_2149_cov_9.993314"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLL14_09465"
     CDS             <1..720
                     /locus_tag="PLL14_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOG04198.1"
                     /translation="VGPGLGRSTVFATLRSTARAAPPVAIEAPDDATPAASPDSPSVR
                     AAESEPALPPEPAESAQATSLVATLGQQRLEAQLPPYLVAPFSIDASQGPWYFPRSEL
                     TVAPKLLDEPLIPQPEKPGTAVQRSGKVVLRVFVGAGGAVDRVEVAGSSLPAVFDEAA
                     TAAFARLRFRPGEIEGVAVNSETRFEIVFDGSETDSSHATDRVSIGDPRRQAAAEPAA
                     ARSVPAGSAAASPAQARPVGR"
     gene            complement(730..1650)
                     /gene="ispH"
                     /locus_tag="PLL14_09470"
                     /gene_synonym="lytB"
     CDS             complement(730..1650)
                     /gene="ispH"
                     /locus_tag="PLL14_09470"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOG04199.1"
                     /translation="MQVILANPRGFCAGVDRAIAIVERAIEKYGAPIYVRHEVVHNLF
                     VCDNLRAKGAIFIDELADVPAGGTVVFSAHGVSKAVREEAAARGLKVFDATCPLVTKV
                     HLEVARMRREGREIVMIGHRGHPEVEGTMGQSGDGMYLVETVADARQLQVKSPASLAY
                     VTQTTLSVDDAAQVVAALKERFPEIIGPKKDDICYATQNRQDSVKQLARNTEVVLVVG
                     SRSSSNSNRLREVAELIGSRAYLVDQAEQIDPEWLSGASRIGVTAGASAPEVLVQNVV
                     DRLSGGDRAKVAQLDGAEENVTFALPRELQ"
     gene            complement(1659..2129)
                     /locus_tag="PLL14_09475"
     CDS             complement(1659..2129)
                     /locus_tag="PLL14_09475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOG04200.1"
                     /translation="MPPPAHATRPANTIATVLADSFLTLHYSLGDRDGDEYVSTFGLS
                     PATLQMGSGQIAETLEQCLLGMAVGERQVFQLAATDAFGAYNPRLVERIARSALPPEI
                     ELRVNSLVEFSLPDGAGNFAGFLRELTDNSALFDFNHPLAGKALRFEVEVIAIM"
BASE COUNT          339 a          672 c          761 g          377 t
ORIGIN      
        1 gtggggccgg gtctgggcag gtcgaccgtg ttcgcaacgc tgcgcagcac agcccgcgcg
       61 gcgccaccgg tagccatcga agcgcctgac gatgcgaccc cggcggcgtc cccagactct
      121 ccttcggtgc gcgctgctga gtcggagcca gcgctgccgc cggagccggc cgaatccgcc
      181 caagcgacgt cactggtcgc gacgttgggc cagcagcggc tggaggcgca gcttccaccc
      241 tacctcgtgg ctcctttctc catcgacgcg tcgcagggtc cctggtattt cccgcgttcg
      301 gaactgacgg ttgcgccgaa gctgctggac gagccgctga tcccgcagcc ggagaagccg
      361 ggcactgccg tgcagcgtag cggcaaggtg gtgttgcggg tgttcgtcgg tgccggcggt
      421 gcggtcgacc gcgttgaagt ggctggcagc agcttgccgg ccgttttcga cgaggctgca
      481 actgcagcct ttgcgcgatt gcgtttccgt ccgggcgaga tcgaaggggt ggcggtcaac
      541 agcgagacgc gtttcgaaat agtgttcgac ggcagcgaaa ccgacagcag ccatgcgacc
      601 gaccgggtga gcattgggga cccgcggcgg caagcggctg ccgagccggc ggcggcccgg
      661 tcggtgcccg cggggagcgc cgcggcctcg ccagcgcagg ctcggccggt ggggcgctag
      721 gccagagcgc tactgcagtt cgcgaggcag ggcgaaggtc acgttttcct cggcaccgtc
      781 gagctgggcg actttcgcgc ggtcgcctcc cgacagacga tcgacgacgt tctgcaccag
      841 aacctcgggc gccgaggcgc cggcggtgac gccgatacgg ctggcaccgc tgagccactc
      901 cgggtcgatc tgttcggcct ggtcgaccag gtaggcgcgg ctgccaatca gttcggcgac
      961 ctcgcggagt cgattcgagt tcgaactcga ccggctgcca acgacgagga cgacttcagt
     1021 gttgcgcgcc agttgtttga ccgaatcctg ccggttttgc gtggcgtagc agatgtcgtc
     1081 cttcttcggg ccgatgattt cgggaaaacg ctccttcaag gcggcaacga cctgcgcggc
     1141 gtcgtcgacc gacaaggtgg tctgcgtgac gtaggccagg ctggccgggg acttgacctg
     1201 gagttggcgc gcgtcggcga cggtttcgac gaggtacatg ccgtcgccac tctgccccat
     1261 cgtgccttcg acctcgggat gtcccctgtg gccgatcatc acgatctcac ggccttcccg
     1321 gcgcattctg gcgacttcga ggtgcacctt ggtgaccagc gggcaggtgg cgtcgaagac
     1381 cttgaggccg cgtgccgcgg cttcctcgcg cacggctttc gagaccccgt gggcactgaa
     1441 aacgaccgtg ccaccagccg gcacgtcggc cagttcgtcg atgaaaatgg cgcccttggc
     1501 acgcaggttg tcgcagacga agaggttgtg taccacttca tggcgaacat agatcggcgc
     1561 accgtacttc tcgatggcac gctcgacgat ggcgatggcg cgatcgacgc cggcgcagaa
     1621 gccgcgggga ttggcgagaa tgacctgcat gctggatctc acatgatggc gatgacttcg
     1681 acctcgaagc ggagggcttt gccggcgagc gggtggttga agtcgaagag cgcgctgttg
     1741 tcggtcagtt cgcgcagaaa accggcaaag ttgccggcgc cgtcgggcaa actgaattcc
     1801 accagcgagt tgacccggag ttcgatctcc gggggcaggg cgctgcgcgc gatacgttcg
     1861 acgaggcgtg ggttgtaggc accaaaggcg tccgttgccg ccagctggaa gacctgacgc
     1921 tcgccgacag ccattccgag caggcactgt tcgagcgttt ccgcgatctg gccgctaccc
     1981 atttgcaggg ttgcgggaga caggccgaag gtgctgacgt actcgtcgcc gtcgcggtcg
     2041 ccaaggctgt agtgcagcgt caggaaactg tcggccagca ccgttgcgat ggtgttcgct
     2101 ggccgggtgg catgggctgg cggcggcatc agcgacctcg ctcgcagcg
//
LOCUS       DAOSIT010000422         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44398_length_2143_cov_12.918582, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000422 DAOSIT010000000
VERSION     DAOSIT010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44398_length_2143_cov_12.918582"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLL14_09480"
     CDS             complement(<1..344)
                     /locus_tag="PLL14_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HOG04201.1"
                     /translation="MSLRQLALDALLVDSPRAKCAAVQSIVRSGEHPAGAAPDVRQPI
                     PAALPGRPARPLLVPPAQLAQRPVNTLEGRAALIHALAHIELNAVNLALDIVWRFDGM
                     PAEFYRDWIGVAA"
     gene            complement(341..823)
                     /locus_tag="PLL14_09485"
     CDS             complement(341..823)
                     /locus_tag="PLL14_09485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOG04202.1"
                     /translation="MHAAIHEFTVERLEGGSQSLGDFAGQVLLIVNTASQCGFTPQYQ
                     GLEALYQRYRERGLVVLGFPCNQFGAQEPGAAAEIASFCQKNYGVSFPMFAKVEVNGD
                     HAHPLYQYLKKAAPGLLGSEGIKWNFTKFLVNRQGEVTGRYAPATAPEAMATDIEELL
                     "
     gene            complement(826..>2143)
                     /locus_tag="PLL14_09490"
     CDS             complement(826..>2143)
                     /locus_tag="PLL14_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG04203.1"
                     /translation="ITWPEFADIHPFAPLDQAAGYLQMIGELETWLKSITGFDAICMQ
                     PNSGAQGEYAGLVAIARYHASRGESRRRVCLIPKSAHGTNPATAQMCGMRVVVVNCDE
                     AGNIDVADLRAKAAQHADQLACLMITYPSTHGVFEEAVQEICAIIHQHGGQVYMDGAN
                     LNAQVGLTSPALIGADVSHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFMANHSVQPI
                     PGPHAGQGAVAAAPWGSASILPIAWMYIAMMGGSGLTRATAVAILNANYVAICLQPHY
                     PVLYKGSHGRVAHECILDIRPIKAATGISEIDIAKRLMDYGFHAPTVSFPVAGTMMVE
                     PTESESRAELDRFIVAMIAIREEIRSIELGHWPADDNPLTHAPHTQADIVDSLWDRPY
                     SRQEAVFPLPYVAENKFWPSVNRIDDVYGDRHLFCACVPVDDQGER"
BASE COUNT          356 a          655 c          729 g          403 t
ORIGIN      
        1 gcggcgacgc cgatccagtc gcggtagaac tcggcgggca tgccgtcgaa gcgccagacg
       61 atgtcgagcg cgagattgac cgcgttcagt tcgatgtggg cgagcgcatg aatcagtgcc
      121 gcacgcccct cgagcgtgtt gaccggtcgc tgcgcgagtt gtgcgggcgg aaccagcagc
      181 ggcctggcgg ggcgaccagg aagtgctgcc ggtatcggtt gccgcacgtc gggcgccgcg
      241 ccggccgggt gttcgccgct gcgcacgatg ctctggacag cggcgcactt ggcgcgcgga
      301 ctgtccacca gcaaggcgtc gagggcgagt tggcgcaggc tcaaagcagt tcttcgatat
      361 ccgtggccat tgcctccggc gcggtggccg gggcgtagcg gccggtcacc tcaccctgac
      421 gattgaccag gaacttggtg aagttccact tgattccctc gctgccgagc aggccaggcg
      481 cggccttctt caggtactgg tagagcggat gggcatggtc gccattgacc tcgaccttgg
      541 caaacatcgg gaagctgaca ccataattct tctgacagaa actggcgatc tctgcggccg
      601 cgcccggttc ctgcgcgccg aactggttgc agggaaaacc cagaaccacc aggccgcgct
      661 cgcgatatcg ctggtaaagc gcttccagac cctgatattg cggtgtaaag ccgcactggc
      721 tggcggtgtt gacaatcagc agaacctggc ccgcgaaatc gccaagggac tggctgccgc
      781 cttcgaggcg ttcgacggtg aattcgtgta ttgcggcgtg catggtcatc tttctccttg
      841 atcgtcgacc ggaacacagg cacagaacag gtggcggtcg ccgtaaacat cgtcgatgcg
      901 attgacgctt ggccagaact tgttttccgc cacgtacggc agcgggaaga ccgcttcctg
      961 gcgactgtac ggcctgtccc agaggctgtc gacgatgtcc gcctgagtgt gaggcgcatg
     1021 cgtcagcggg ttgtcgtcgg ccggccagtg gcccagctcg atgctgcgga tttcttcgcg
     1081 gatggcgatc atcgcgacga tgaagcggtc gagctcggct ctggattccg actcggtggg
     1141 ttcgaccatc atcgttccgg caaccggaaa actgaccgtc ggcgcgtgaa agccgtagtc
     1201 catcagccgc ttggcgatgt cgatttccga gattccggtc gccgccttga tcggacgaat
     1261 gtcgagaatg cattcgtgcg cgacgcgccc gtggctgccc ttgtacagca ccggatagtg
     1321 gggctgcagg cagatggcga catagttggc gttgaggatg gcgacggcgg tggcgcgcgt
     1381 cagcccgctg cctcccatca tcgcgatata catccacgca atcggcagga tcgacgccga
     1441 tccccaggga gccgcggcga ccgcgccctg gccggcgtgt ggaccaggga ttggctgcac
     1501 gctgtggttg gccatgaagg gtgccagatg ggccttgagg ccgatcggtc ccatccccgg
     1561 cccgccacca ccgtgcggga tgcagaaggt cttgtgcaga ttcatgtggc tgacgtcggc
     1621 accgatcagg gccggcgagg tcaggccgac ctgcgcattg aggttggcgc cgtccatata
     1681 gacctggccg ccgtgctggt ggatgatggc gcagatttcc tgtaccgctt cctcgaagac
     1741 gccgtgcgtc gagggatagg tgatcatcag gcaggcgagc tggtccgcat gttgcgccgc
     1801 cttggcccgg aggtcggcga catcgatatt gccggcctcg tcgcagttga ccaccaccac
     1861 ccgcatgccg cacatctggg cggttgccgg attggtgccg tgtgccgatt tcgggatcag
     1921 gcagacccgg cgccggctct cgccgcggct ggcgtggtag cgcgcgatcg ccaccagacc
     1981 ggcgtactcg ccctgtgccc ccgagttcgg ttgcatgcag atggcgtcaa agccggtgat
     2041 cgacttgagc caggtttcca gctcgccgat catttgcagg taccctgccg cctgatcgag
     2101 cggcgcgaag gggtggatgt cggcgaactc cggccaggtg atc
//
LOCUS       DAOSIT010000423         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44542_length_2138_cov_9.750360, whole genome shotgun sequence.
ACCESSION   DAOSIT010000423 DAOSIT010000000
VERSION     DAOSIT010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44542_length_2138_cov_9.750360"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1471
                     /locus_tag="PLL14_09495"
     CDS             155..1471
                     /locus_tag="PLL14_09495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG04204.1"
                     /translation="MVRIRKLILPVRKMLGGTLRKLFNFLPRERRFAVYRNFVDCDPA
                     PDERLVLKIAETKEELEACFTLLHDAYVGSGFMKPHPSGMRVTIYHALPTTTTLCAKY
                     DGQVVGTLSLIRESVLGFPLQRIFDLTAIREKKGNVAEVSALAVHKRFRRTGGSILFP
                     LMKFMREYCTSFFDVRHLVIAVNPSHIQMYESLLFFQRLTQNVVENYDFVNGAPAVGA
                     TLDLRAAPEIFRKHYASKPPRRNLHAFFWQLEMPNIKMPARRFYTTNDPVLTPDLLDY
                     FFNVRTSVFSELSDRKKCLLHTIYDLPAYRAVLPVLAEGEQDRSVRRRHRRFSVRCPA
                     RFSVVGADGKESKVSLEIVQLSRYGFQAHALTAVPIDVWGEVTIQLGPAEVSRIQVSA
                     SRESSGGMYGFRLGEPDLIWRKFVNALYSGSTHADLENATRFMTDR"
     gene            complement(1509..>2138)
                     /locus_tag="PLL14_09500"
     CDS             complement(1509..>2138)
                     /locus_tag="PLL14_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG04205.1"
                     /translation="LRLLHGLFSWLVGRSIVLDSVAFPYPRPQHADDYALIYTANSTF
                     DAAALRALFAANLLDLPVRRDEAALQGFLEGAPGKLSTLYRRDREMVLRVRKVLRDAL
                     PASCSLGDVARALYLSPRTLHRRLEDEGSSFQAIKDALRRDLATNRLAKTRQPVAQIA
                     AELGFADTAAFYRAFLRWTSMAPAHYRRRLQAAATGGAVSTAQQIPKPP"
BASE COUNT          398 a          626 c          682 g          432 t
ORIGIN      
        1 gctgttctgc acttttcttc cggattttgg gtacagtaca cttgcaccag actaattggg
       61 ttccggcagt ctatgggagg agaagccggg ggttttcgtg catcgatggg gagagcgtgc
      121 atttcggacc gtaatcgttg ctgaggggat ggggatggtg aggatcagga agctaatcct
      181 gccggtgcgg aagatgctgg gcgggacgct gagaaagctg ttcaactttc tcccacgcga
      241 acggcgtttc gcggtttacc gcaactttgt cgactgtgac ccggctccgg acgagcggct
      301 ggtactgaag atcgccgaga cgaaagagga actggaggcg tgcttcacct tgctgcacga
      361 cgcctatgtc ggcagcggat tcatgaagcc gcatccttcc gggatgcggg tcaccattta
      421 ccatgccctg ccgacgacca cgaccttgtg cgcgaagtac gatgggcaag tggtcgggac
      481 gctgtcgctg atcagggaaa gtgttctcgg ctttccgctg cagcggattt tcgatctgac
      541 ggcaatccgc gagaagaagg gaaatgttgc cgaggtatcg gcgttggcgg ttcacaagcg
      601 tttccgccgc accggcggca gcatcctgtt cccgcttatg aagttcatgc gcgagtattg
      661 caccagtttc ttcgatgttc ggcatctggt gattgccgtc aaccccagtc atatccagat
      721 gtacgagtcg ctgctcttct tccagcgcct gacccagaat gtggtcgaaa attacgactt
      781 cgtgaatggt gcgccggcgg taggtgcaac cctcgacctg cgggccgctc cggaaatctt
      841 ccgcaaacac tatgcgtcga agccgccacg gcggaatctg catgcatttt tctggcagct
      901 cgagatgccc aacatcaaga tgccggcgcg ccgcttttac accaccaacg acccggtact
      961 gacccccgat ctgctcgatt actttttcaa tgtacgcacc agcgtcttca gtgagctgag
     1021 tgatcgcaag aagtgcctgc tacatacgat ctacgatttg cccgcctacc gagcggtgct
     1081 gccggtgctt gcagagggcg agcaggatcg cagtgtgcgc aggcgtcacc ggcgattttc
     1141 ggtacgctgt ccggctcggt tttctgtggt cggcgccgac ggcaaggaga gcaaggtctc
     1201 ccttgagatc gtgcagcttt cgcgctacgg tttccaggcg cacgcactga ctgccgttcc
     1261 gatcgacgtc tggggcgaag tcacgatcca gctggggccc gccgaagtct cgcgcatcca
     1321 ggtttcggcg tcccgggaat ccagcggtgg catgtatggc ttccgtcttg gtgaacccga
     1381 cctgatctgg cgcaagttcg tcaatgccct gtacagcggc agtacccacg ccgacctcga
     1441 aaacgccact cgcttcatga ccgaccgttg aggtccctcg gtcgccgccg ccgtccctcc
     1501 cgagcggttc agggtggctt gggaatctgc tgtgcggtgc tcaccgctcc tccggtcgcc
     1561 gctgcttgca gcctgcggcg gtagtgcgca ggggccatgc tggtccaccg caggaacgcg
     1621 cggtagaaag ccgccgtgtc ggcaaaaccg agttcagcgg caatctgggc caccggctgg
     1681 cgggtcttgg ccaggcgatt ggtggccagg tcgcggcgca aggcgtcctt gatcgcctga
     1741 aagctggaac cttcgtcttc caggcggcga tgcagggtgc gtggcgacaa atacaaggcg
     1801 cgagcgacgt caccgagcga acaggacgcc ggcagtgcat cacgcaggac cttgcgcacg
     1861 cgcaggacca tttcacggtc acggcgatag agtgtgctca gtttgcccgg agcgccttcg
     1921 aggaagccct gcaacgcggc ttcgtcgcgc cggacaggca agtcgagcag gttggcggca
     1981 aacagcgcac gcagtgctgc ggcgtcgaaa gtcgagttcg ccgtgtagat cagcgcataa
     2041 tcgtccgcat gctgcggtcg cgggtagggg aaggcgaccg aatcgagaac gatgctgcgc
     2101 cccaccagcc aggagaagag accgtgcagc aggcgcag
//
LOCUS       DAOSIT010000424         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44625_length_2135_cov_6.568750, whole genome shotgun sequence.
ACCESSION   DAOSIT010000424 DAOSIT010000000
VERSION     DAOSIT010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44625_length_2135_cov_6.568750"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..835)
                     /locus_tag="PLL14_09505"
     CDS             complement(140..835)
                     /locus_tag="PLL14_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HOG04206.1"
                     /translation="MSAFFRFPHTPHLAWMGGGIPRDDKVLSAVEADALLAGPVVVEE
                     KLDGANLGFSVSLDGVLRAQNRGHYLDAPHAGQFARLGQWLAPREGALFDALGEHLVA
                     FGEWCAARHSLAYTALPDWWLLFDVYDRRAGRFWSTERRDQWAGRLGLITVPALMRGQ
                     RSLQELRQMLINHRSAFREGPLEGVIIRREDESWLQSRAKLVRADFTQQIAEHWRHRS
                     LEWNRLGDVVTEG"
     gene            complement(832..1371)
                     /locus_tag="PLL14_09510"
     CDS             complement(832..1371)
                     /locus_tag="PLL14_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04207.1"
                     /translation="MTPVVIDTNVLLVANGSHQDVSCKCREACVSALLARQRSGMVVI
                     DDGYRILREYGNRTYPNQPKGVGDAFLKWLLQNHSNATRVCRVPITETGQDRFVEFPD
                     PLLEERFDPPDRKFAAVANAHPDKPPIWQGADSKWLDWWPQLAAQGISVHFLCPADVE
                     RFYAKKFPERDLPAFPPRP"
     gene            complement(1368..>2135)
                     /locus_tag="PLL14_09515"
     CDS             complement(1368..>2135)
                     /locus_tag="PLL14_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HOG04208.1"
                     /translation="EEMLERMYYLGPLRQSARRVYQWSGETPPHVGALGEHTIAALLA
                     ATEQGRKLNRGPKQKLQPFDVFIAGWLRDLGVIDSFEVHPVAEGRKEYEVLVRTHAKS
                     PVVKLTDVGVGVSQVLPALVQAFYSPPNSVVWMEQPEIHLHPSAQANLADAFVSAVQS
                     NENGSARGTQLIIETHSEHFLTRLQRRVAERRIEATDVAVYFVKREGASAELEALRLN
                     IFGEIENWPVNFFGDEMGDIAARTLEAIKRQAAGETA"
BASE COUNT          412 a          716 c          622 g          385 t
ORIGIN      
        1 catgaccata caatacggct ccacggcgac ggagtcaagt gtgtgttacg gtcagggtcg
       61 aggaggggtg cgacggtttc acctgcggaa gatctcccga cgatgccagg cgagttgatt
      121 tcgatccgcg ttagtcactt cacccttctg tcacgacatc gcccaagcgg ttccattcca
      181 gggaccggtg ccgccagtgc tcggcaattt gctgcgtgaa atccgcccgc accagcttgg
      241 cccgcgactg aagccaggat tcgtcctcgc gccggatgat caccccctca agcggtccct
      301 cgcgaaaggc gctccggtga ttgatcaaca tctgccgcaa ctcctgcaaa ctacgctggc
      361 cacgcatcag tgcgggaaca gtgatcaggc ccaatcgccc ggcccattgg tctcgccgct
      421 cggtgctcca gaagcgaccg gctcgccggt cgtaaacatc gaagagcaac caccagtcgg
      481 gcagggccgt gtaagcaagt gagtggcgcg ctgcgcacca ctccccgaag gcgacaagat
      541 gttcgccaag tgcatcgaac aatgccccct cgcgcggggc cagccactga cccagacgcg
      601 cgaactggcc ggcatggggt gcatcgaggt aatgcccacg gttctgagcg cgcaggacgc
      661 catccagcga aaccgagaat ccgagattgg cgccatcgag cttttcttcc accaccaccg
      721 gccctgcgag cagagcatca gcctcgacgg cagacagcac cttgtcgtcg cgcgggatgc
      781 cgcctcccat ccaggccagg tgcggcgtgt gcggaaaacg gaaaaaggcg ctcatggcct
      841 cggtggaaaa gccggcagat cgcgctccgg aaacttcttc gcgtagaacc gctcgacgtc
      901 cgccggacag agaaaatgga ccgaaatgcc ttgggctgcc agttgcggcc accagtccag
      961 ccacttgctg tcggccccct gccagatcgg tggcttgtcc ggatgcgcgt tcgccacggc
     1021 cgcgaacttc ctgtcgggag ggtcgaaacg ctcctccagc aaaggatcgg gaaactctac
     1081 gaaacggtcc tggccggtct cagtgatcgg cacacggcac acgcgggtgg cattggagtg
     1141 attctgcagc agccacttga gaaaggcgtc acccaccccc tttggctgat tcggatacgt
     1201 cctgtttccg tactcgcgga gaatgcggta accatcgtcg atcaccacca tgccggaccg
     1261 ctgcctggcc agcagcgcgc tgacgcaagc ttcacggcac ttgcaggaga cgtcctggtg
     1321 gctgccgttg gccaccagca acacgttggt atcgatcacc accggcgtca tgccgtctcc
     1381 cccgcggcct gacgcttgat cgcctcaagc gtgcgcgccg cgatgtcgcc catctcgtcg
     1441 ccaaagaagt tcactggcca gttttcgatc tcgccgaaga tattgagacg caaggcctcc
     1501 agttccgcgc tcgcgccctc gcgcttgacg aaatacaccg ctacgtcggt cgcctcgatt
     1561 ctgcgctccg ccacccgacg ttgcagacgt gtgaggaaat gctctgagtg cgtctcgatg
     1621 atcaactggg tgccgcgcgc gctgccgttc tcgttggact ggaccgcact gacgaaagca
     1681 tccgcaaggt tggcctgggc cgaaggatgc aggtggattt ccggttgctc catccacacc
     1741 accgaattgg gcggtgaata gaatgcctga accagtgccg gcaacacctg cgagacgcct
     1801 accccgacgt ccgtcagctt gaccaccggc gacttggcat gggtccgtac caaaacctcg
     1861 tattccttgc gtccctctgc caccgggtga acctcgaaac tgtcgatcac ccccaggtcg
     1921 cgtaaccagc cagcgatgaa cacgtcgaac ggctgcagct tctgcttcgg gccacgattc
     1981 agcttgcgcc cctgctcggt cgccgccagc agtgcggcga tggtatgttc gcccagcgca
     2041 ccgacatgtg gcggagtctc gcccgaccac tggtagaccc ggcgtgccga ctgccgcaac
     2101 ggtccgaggt agtacatccg ctccagcatt tcttc
//
LOCUS       DAOSIT010000425         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44775_length_2130_cov_7.490602, whole genome shotgun sequence.
ACCESSION   DAOSIT010000425 DAOSIT010000000
VERSION     DAOSIT010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44775_length_2130_cov_7.490602"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1283
                     /gene="ispC"
                     /locus_tag="PLL14_09520"
     CDS             96..1283
                     /gene="ispC"
                     /locus_tag="PLL14_09520"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG04209.1"
                     /translation="MQQLTILGSTGSIGVNTLDVLRRHPQRYRVVALCAHRQVDLLFA
                     QCLEFRPRHAVVGDAVLAADLAARLLAAGCPTEVGHGPGALVRMAELPEVDAVMAAIV
                     GAAGLPPTLAAAVAGKKILLANKEALVMAGPVFMRAVRENGATLLPIDSEHNAIFQSL
                     PSTYSGDPAGSGVLGLFLTASGGPFRELALSDFEAVSPEQACAHPNWIMGRKISVDCA
                     TMMNKGLEVIEAHWLFNVPADNIQVVIHPQSVIHSLVQYADGSVIAELGNPDMRTPIA
                     HALAYPQRIAAGVEALDLYKVASLHFERPDLDRFPCLGLAYRALREGGSAPTTLNAAN
                     EVAVQAFLDRRIRFTAIARVIAEVMDRLPTGGMPASLAEVLAVDRTARAAAEHLIMDP
                     AFR"
     gene            1280..>2130
                     /gene="rseP"
                     /locus_tag="PLL14_09525"
     CDS             1280..>2130
                     /gene="rseP"
                     /locus_tag="PLL14_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG04210.1"
                     /translation="MSGFLYYLAAFALVLGILVVVHEFGHYLAARWAGVKVLRFSVGF
                     GRPLWARRFGQDGTEWAIAAFPLGGYVKMLDEREGEVPAEELHRSFNRQPVLRRMVIV
                     VAGPAANFLLAIVLYWGLFWNGTEEFKPLLGAPAAESAAASAGLEDGELVLKVAGESV
                     QTWQEMRWVLLQRAVEQDQIELEVINPQHEIALRRLDVLAVRQSGWEGEALDRLGLTL
                     YRPRLPPVLGQVNANSAAADAGLRPGDEVLSIDEIPIGSWADVVQHVRKSPGKAIHLE
                     VLRDGVRV"
BASE COUNT          339 a          632 c          731 g          428 t
ORIGIN      
        1 ggggtgctgg accgcatcga cagtctcacg tcgacactgc cgctggtcgc tctggtctgg
       61 ctggcgacac agccttgata ccgtcagggt cgactgtgca gcagctcacg attctcggct
      121 cgacagggtc catcggggtc aatacgctgg atgtcctgcg acgtcaccca cagcgctacc
      181 gggttgtggc actttgcgcg caccgtcagg tggacctgct gttcgcacag tgcctggagt
      241 ttcgaccgcg ccatgcggtt gttggcgatg cggttctggc cgccgatctc gccgcgcgct
      301 tgctggccgc gggttgtccg acagaggtgg ggcatggtcc gggcgcgctg gtgcgcatgg
      361 cggaactccc ggaggtggat gcggtgatgg ccgcgatcgt cggcgcggcg ggtctgccgc
      421 ccaccttggc ggcggctgtc gccgggaaga agatccttct ggcgaacaag gaagcactgg
      481 tcatggccgg cccggtcttc atgcgtgccg tgcgcgaaaa cggtgcgacc ctgctgccga
      541 tcgacagcga gcacaatgcc attttccagt ctctcccgag cacctactcg ggcgatccgg
      601 caggcagtgg cgtgcttggg ctgttcctta cggcatcggg tggtcccttt cgtgaactgg
      661 cactcagcga ctttgaagcc gtcagccccg aacaggcgtg tgcccatccg aactggatca
      721 tgggacgaaa gatctcggtc gactgcgcga cgatgatgaa caagggcctg gaggtgatcg
      781 aggcgcactg gcttttcaac gttccggcag acaacattca ggtggtcatc catccccaaa
      841 gcgtcattca ttcgctcgtt cagtatgccg atggctcggt catcgcggaa ctcggtaatc
      901 cggacatgcg cacgccgatc gcgcacgccc tggcttatcc gcagcgcatc gcggcgggcg
      961 tcgaggcgct tgatctgtac aaggtggcgt cgctgcattt cgagcgtccc gacctggatc
     1021 gctttccgtg cctgggactg gcctatcgtg cgctcaggga gggcggcagt gcgccgacga
     1081 cactgaatgc ggcgaacgaa gtggcggtgc aggccttcct cgaccgcagg atcaggttta
     1141 cggcgattgc acgggtgatt gccgaagtca tggatcgctt gccgaccggc gggatgcctg
     1201 cctcactggc cgaggtcctg gctgtcgacc gcacggcgcg cgcagcggct gaacatttga
     1261 tcatggatcc agctttccga tgagcggctt tctttactat cttgcggcct ttgcgctggt
     1321 cctgggaatc ctcgtcgtgg tgcacgagtt cgggcactat cttgccgcgc gctgggcagg
     1381 ggtcaaggtc ctgcgcttct cggtcggctt cggtcggcca ctctgggcgc ggcgctttgg
     1441 ccaggatggc accgagtggg caattgccgc tttcccgctc ggtggttatg taaaaatgct
     1501 cgacgaacgt gaaggcgagg ttcctgccga ggagttgcac cggagcttca accggcaacc
     1561 tgtcctgcgg cggatggtga ttgtcgtagc ggggccggca gccaactttc tgctggccat
     1621 tgttctttac tggggactgt tctggaacgg aacggaagag ttcaagccgc ttctgggcgc
     1681 gcccgccgcc gagagtgcgg cggcgtcggc aggactggaa gacggtgaat tggtgctcaa
     1741 ggttgccgga gaaagcgtgc agacctggca ggagatgcgt tgggtcctgc tgcagcgggc
     1801 cgttgagcag gatcagatcg aacttgaggt gatcaacccg caacacgaga tcgcgttgcg
     1861 gcgccttgac gtgctggccg ttcgacagtc gggctgggaa ggcgaggcgc tggaccggct
     1921 gggtctcacg ctttaccgcc cccggctgcc ccccgtgctg ggccaggtca atgccaacag
     1981 tgccgccgcc gatgccgggc tgcgtcccgg agacgaggtg ctcagcattg acgaaatacc
     2041 gatcggcagt tgggccgatg tcgtgcaaca cgtccgcaag tcgcccggga aagcgattca
     2101 tctggaggtg ctgcgcgacg gcgtgcgcgt
//
LOCUS       DAOSIT010000426         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44939_length_2125_cov_5.822705, whole genome shotgun sequence.
ACCESSION   DAOSIT010000426 DAOSIT010000000
VERSION     DAOSIT010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44939_length_2125_cov_5.822705"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1335)
                     /locus_tag="PLL14_09530"
     CDS             complement(55..1335)
                     /locus_tag="PLL14_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOG04211.1"
                     /translation="MADHQYGFDTLCLHAGQIPDAATGSRAVPIYQTTSYVFDSPEHA
                     ASLFNLQTFGNVYSRLSNPTVAVLEERLAAIEDGRAAVATGSGMAAQMIALLNLCAAG
                     DEIVAARTLYGGTHTQLDVNFRKLGINTIFVDPDDPENFARAITPKTRLIYAETMGNP
                     SLNVLDIEAVARLGSQAGLPLFIDNTFASPYLCQPFRWGAAISLHSATKFIGGHGTTM
                     GGIIVESGKFPWDNGKFATMTEPSPGYHGVRFYETFGDFGFTMKCRMEGLRTFGPALS
                     PLNAFLLLQGIETLPLRMERHCSSALAVARFLLDHPKVAWVNYPGLKNNRYYDLANKY
                     LPKGAGAVLSFGIRGGAAAGQKFIDSVGFLSHLANVGDAKTLVIHPASTTHRQLSEEQ
                     QVAAGVPPDLIRLSVGLETLDDILWDIDQALAKS"
     gene            complement(1345..1815)
                     /locus_tag="PLL14_09535"
     CDS             complement(1345..1815)
                     /locus_tag="PLL14_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOG04212.1"
                     /translation="MSDINTLRRVLRESRTLAIVGLSADWFRPSNFVAKYMQEHGYRV
                     IPVNPKYDEILGEKCYPTLSAIREPVDLVDVFRKPADTPEIARESVAIGARTLWLQIG
                     VINDEAVKIAEESGLTVVMGRCVKIEYARLFGGLNWFGVNTRLISSRRPTWLPH"
BASE COUNT          430 a          602 c          663 g          430 t
ORIGIN      
        1 cctcgggaca atgacaaata actcgtattg ttcaagttat ttttgcttgc gtccttagct
       61 cttggccagc gcctggtcga tatcccaaag aatatcatcg agtgtttcca gtccgaccga
      121 gagtcggatc agatcgggcg gcacgccggc ggccacttgt tgctcttcgg agagctgacg
      181 gtgtgtcgtc gacgccgggt gaatgaccag ggtcttggca tcaccgacgt tggccaggtg
      241 ggaaaggaat ccgacgctgt cgataaactt ctgcccggcc gcagcgccgc ccctgatacc
      301 gaaactgagc accgcgccgg cacctttcgg caggtattta ttggccaggt cgtaatagcg
      361 gttgttcttc agccccggat aattgaccca ggccaccttc gggtgatcca gcaggaagcg
      421 ggcgactgcc agcgcgctgc tgcaatggcg ttccatgcgc agcggcaagg tttcgatccc
      481 ctgcagcagc aggaaggcat tcagcgggga aagcgccggg ccgaaggtgc gcaggccttc
      541 catgcgacat ttcatcgtga acccgaagtc accgaaggtc tcgtagaatc gtacgccgtg
      601 gtagcccggc gagggttcgg tcatggtcgc gaacttgccg ttatcccaag gaaattttcc
      661 ggattcgacg atgatgccgc ccatcgtcgt gccgtggcca ccgatgaact tggtcgccga
      721 gtgcaatgaa atggcggcgc cccacctgaa aggctggcac agataagggc tggcaaaggt
      781 gttgtcgatg aacagcggca ggccggcctg gctgccgagt cttgccaccg cctcgatatc
      841 gagcacattg agcgacggat tgcccatcgt ttcggcgtag atgagcctgg tttttggtgt
      901 gatggcgcgg gcgaagtttt ccggatcatc cgggtcgacg aaaatggtat taatcccgag
      961 cttgcggaaa ttcacgtcga gctgggtatg cgtgccaccg tacagggtgc gggctgcaac
     1021 aatctcgtcg ccagccgcgc acaggttgag cagggcgatc atctgggcgg ccatgccact
     1081 gccggtagcg actgcggcgc ggccgtcctc gattgccgca aggcgttctt cgagcaccgc
     1141 gacggtcgga ttggacagcc gtgaatacac attgccgaag gtttgcaggt tgaacaggct
     1201 ggccgcatgc tccgggctgt cgaagacgta ggatgtggtc tggtagatgg gcacagcgcg
     1261 gctgcctgtg gcggcgtcgg gaatctggcc ggcgtgcaga cagagggtat cgaacccgta
     1321 ttggtgatca gccatggaaa actttcagtg aggcagccag gtcggccggc gggaggaaat
     1381 caggcgggta ttgaccccga accagttgag accgccgaac aggcgggcgt attcgatctt
     1441 gacgcagcgg cccatcacga cagtcaaccc ggattcctcg gcaattttca cggcctcatc
     1501 gttgatcacg ccgatctgca gccacaaggt cctggccccg atggcgaccg actcccgtgc
     1561 gatctccgga gtgtcggccg gcttgcggaa cacatcgacc agatcgaccg gttcgcgaat
     1621 cgccgacagc gtcgggtagc acttctcgcc caggatctcg tcgtacttcg gattgaccgg
     1681 gatgacccgg taaccgtgtt cctgcatata cttggccacg aagttggagg ggcggaacca
     1741 gtcggcggaa agacccacga tggccagggt acggctttca cgaagcacgc ggcgcagagt
     1801 gttgatatcg ctcatgggtg tgatcctggt gaactaaagg taccggaatg acgatcgttg
     1861 catcaaccag aaaagtatca tcgttcttct cgacggcgac caggatctcg ggttcggctc
     1921 cgaacgctgc aaagcacatg aattcaagtt ctgattgaac gaactgccga tgacattcac
     1981 ggcgagcact gctagcgaac ggccgcctgt agcttgcgca gttcgatttc agcccagagt
     2041 gctttgccaa acgccttcca ggctgggccg ccgtggcatt cgaatccgac accggcacat
     2101 tcctccatcg tcttgagcat gatgg
//
LOCUS       DAOSIT010000427         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_44999_length_2123_cov_10.166828, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000427 DAOSIT010000000
VERSION     DAOSIT010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44999_length_2123_cov_10.166828"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLL14_09540"
     CDS             complement(<1..758)
                     /locus_tag="PLL14_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOG04213.1"
                     /translation="MSASLIKVPQGGQKIIPGQAVPDNPIIPFIEGDGIGIDITPVMI
                     KVVDAAVAKAYGGAKQIHWMEVYAGEKSTQMYGSDVWLSAETLDTLKEYSVSIKGPMT
                     TPVGGGIRSLNVALRQELDLYQCVRPIQYFRGVPSPLKDPSKTNMVIFRENTEDIYAG
                     IEWEAGSEGCNKVVKFLQEEMGVKKIRFPATSGIGIKPISKDGTERLVRAAIKYAIAN
                     KRRSVTIVHKGNIMKFTEGAFRDWSYALAKNEFGG"
     gene            1067..>2123
                     /locus_tag="PLL14_09545"
     CDS             1067..>2123
                     /locus_tag="PLL14_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOG04214.1"
                     /translation="MDIFSVENALAQRIAEAMIDGFNRHYRLIRRYGQQAKALFEAAD
                     WKGVHDAVRERIRSYDDRVRETAELLCANFGAATLDDATWQQLKLFYIGHLINHQQPE
                     LAETFFNSVCSKILHRTYFNNDYIFARSAVSTEYIQSYPPTYRSYYPAQHGLRNTIKQ
                     IIVDFDWQRPFEDLDRDVDYIMRTAELRLGEWPEAEANAQIQVLHSGFYRNKGAYIFG
                     KAINGHFECPFAIAVLHTPENKLVLDTILLDRWLISVLFSLSRAYFMVDMEVPSGYVH
                     FLRSIMLNKPPSEIYTMLGLGKQGKTLFFRDLKQHLRHSEDQFIIAPGIAGLVMLVFT
                     LPSYPYVFKLIKDVFGAS"
BASE COUNT          427 a          653 c          589 g          454 t
ORIGIN      
        1 ccaccgaatt cgttcttggc cagcgcgtac gaccagtcac ggaaggcgcc ttcggtgaat
       61 ttcatgatgt tgcccttgtg cacgatggtc accgagcggc gcttgttggc gatcgcgtac
      121 ttgatggcgg cacggaccag gcgctcggta ccatccttgg aaatcggctt gatgccgata
      181 ccggaggttg ccgggaaacg aatcttcttg acgcccatct cttcctgcag aaacttgacg
      241 accttgttgc agccctcgga accggcttcc cattcgatgc cggcgtagat gtcctcggtg
      301 ttctcgcgga agatcaccat attggtcttc gagggatcct tcagcggcga gggaacgcca
      361 cggaagtact ggatcgggcg gacgcattga taaaggtcga gttcctggcg cagcgccaca
      421 ttcagcgaac ggatgccgcc accgaccgga gtcgtcatcg gtcccttgat cgacaccgag
      481 tattccttga gcgtatcgag cgtctcggcc gacaaccaca cgtcggaacc gtacatctgg
      541 gtggactttt cgccggcata cacttccatc cagtgaatct gcttggcacc tccgtaagcc
      601 ttggcaacgg ctgcgtcgac gaccttgatc atcaccggcg taatgtcgat gccgatgccg
      661 tcgccttcga tgaacgggat gatcgggtta tccgggaccg cttgccctgg aatgattttc
      721 tggccgcctt ggggaacttt gatcagactt gcgctcatgc caactccgtg tagtgtggtg
      781 tggtgatacc cgattctggt gccggcgccg gcgctttgca gttcgctgcg ggggctcgga
      841 agctgcagat cggacgatgc tgcgtacatg ggcacgaggg atctgtctga agcagactcg
      901 cctgcggcac cattcgtgat tatgtcgcaa attcgggtgc tctgccagca tattccggac
      961 cgtcaacccg gttgccgtcc gacctgcggc gccatgccgc cgcgtgatgc gacgcaacag
     1021 cgccgatgct acaatccttc gacgccacac catggaaccc tgccgcatgg acatcttcag
     1081 cgtagaaaac gcgctcgctc aacgcattgc cgaagcgatg atcgacggtt tcaaccggca
     1141 ctaccggctg atccgtcgct acggtcagca agccaaggcg cttttcgagg ctgccgactg
     1201 gaagggcgtt catgacgcgg tgcgcgagcg catccgttcc tacgacgacc gcgtcaggga
     1261 gaccgccgag ttgctgtgcg ccaatttcgg tgccgcgacg ctcgacgatg ccacctggca
     1321 acagctcaag ctgttctata tcgggcatct gatcaaccac cagcaaccgg aactcgcgga
     1381 aacattcttc aactcggtgt gctcgaaaat ccttcaccga acctacttca acaacgacta
     1441 catctttgcc cgctcggcag tgtcgacgga gtacatccag tcgtacccgc cgacctaccg
     1501 gagttactat ccggcacagc atggtctgcg caacacgatc aaacagatca tcgtcgactt
     1561 cgactggcaa cgacccttcg aagatctcga ccgcgatgtc gactacatca tgcgtaccgc
     1621 cgagttgcgg cttggcgagt ggccagaagc ggaagccaac gcgcagatac aggttctgca
     1681 ctcgggcttc tatcgcaaca agggcgccta catcttcggc aaggcgatca acggccactt
     1741 cgagtgccca ttcgccatcg ccgtcctgca cacgccggaa aacaaactgg tcctggatac
     1801 gatcctgctc gatcgatggc tgatcagcgt gctgttctcg ctgtcgcgag cgtatttcat
     1861 ggtcgatatg gaagtccctt cgggctatgt gcactttctg cgcagcatca tgctgaacaa
     1921 accgccttcc gagatctaca ccatgctggg tctcggcaaa cagggcaaaa cgctgttctt
     1981 ccgggatctg aaacaacacc tgcgccactc cgaagaccag ttcatcattg cgccggggat
     2041 tgccggcctg gtcatgctgg tgttcacact gccgtcgtac ccctacgtgt tcaaactcat
     2101 caaggacgtc ttcggggcgt caa
//
LOCUS       DAOSIT010000428         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_45206_length_2116_cov_8.859777, whole genome shotgun sequence.
ACCESSION   DAOSIT010000428 DAOSIT010000000
VERSION     DAOSIT010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45206_length_2116_cov_8.859777"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..825
                     /locus_tag="PLL14_09550"
     CDS             223..825
                     /locus_tag="PLL14_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOG04215.1"
                     /translation="MNYTFASATILLILITDPIGNIPIFANALRHVAPERRPWVILRE
                     VLIAFVLLLTFMFVGEGFLRMMNLSELSLQIGGGVILFLIALRMIFPPPSADAPEAAG
                     EPLIVPLAIPAIAGPSALATVLLLVSQAPDRRTEWIGALSITMAVSAIILVLAERIQR
                     IAGDRFVVALERLMGLVLVAMSIEMMLRGIKTFVMQLTHP"
     gene            917..1405
                     /locus_tag="PLL14_09555"
     CDS             917..1405
                     /locus_tag="PLL14_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04216.1"
                     /translation="MSKRTHAMPWRNIAVLAVAVASGAGCYVVPTTAPDGTVYYSHYP
                     LPPVGTPIAVPPPATSTAPATLPVKLYPSNDQASHTGIVSGSVTNMMTGKGRFVVNYL
                     GEVLGGEATRVSNDERRGVASAFSPGGMYMSCEYQMNTPYQGAGTCSFANGARYQMHI
                     GN"
     gene            complement(1406..1939)
                     /locus_tag="PLL14_09560"
     CDS             complement(1406..1939)
                     /locus_tag="PLL14_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOG04217.1"
                     /translation="MRRLLKNYLPDRESILRNRWLRPFQAVLLHPRLWHLNRHSVSGA
                     VAVGLFCGLIPGPLQMLGAALGALLLRVNLPLALLVTLYSNPFTIVPLYLLAYQIGRQ
                     LTGDDSGFLLPPEFSATAVAAWAQAMQMWMLEVAEPLAVGLVALASSLAVLGYMVTRT
                     AWRLYLINAWRQRKPGS"
BASE COUNT          410 a          667 c          631 g          408 t
ORIGIN      
        1 ctaagtcagc ggcctcgatt ctacgacctt gtcgtgccaa cgccagtcct tcctgccaac
       61 ggcagcagga ggtgccggat tgggcaaacc caggctggcc tgttattctt cgcggatacg
      121 cagccgttct tgcgtcattg gcaaacccgg cccgcccaac tcgcgcaacg catgcccacg
      181 catggccgcc tggagcggga atatttgcgg agtcatgcgg ccatgaacta cacctttgct
      241 tcggcaacga tcctgctgat tctgatcacc gacccgatcg gcaatatccc gatcttcgcc
      301 aacgcgctcc ggcacgtggc cccggaacgc cgaccgtggg tgatcctgcg cgaggtgctg
      361 attgccttcg tactgctgct gaccttcatg ttcgtcggcg agggtttcct gcggatgatg
      421 aacctgagcg agttgtcgct gcagatcggt ggtggcgtga tcctcttcct gatcgcattg
      481 cgcatgatct ttcccccgcc aagcgccgat gcccccgaag ccgctgggga accgctgatc
      541 gtgccgctgg ccattccagc catcgccggg ccttcggccc tggccaccgt gctcctgctg
      601 gtctcgcagg cacctgaccg gcgcacggaa tggattggcg cactgtcgat caccatggcg
      661 gtgagcgcga tcatactggt gcttgccgaa cgcatccaga gaatcgccgg cgatcgcttc
      721 gtggtcgcac tcgaacgtct gatgggcctg gtactggtcg ccatgtcgat tgagatgatg
      781 ctgcgcggca tcaagacctt tgtcatgcaa ctcacgcacc cctgaggcga tttagacgcg
      841 tcaagccatt gcactctact atacttggat tatgggatcg ccatgagatc cgaacgctca
      901 aggaaaggag aacaccatgt cgaaaagaac acatgccatg ccttggcgaa atatcgccgt
      961 gctggctgtg gccgtggcca gcggagcggg gtgctatgtg gtaccaacga cggcgccgga
     1021 cggcacggtt tattacagcc attacccctt gccgccggtg ggaacgccga tagcggtgcc
     1081 gccgccagcc acgagtaccg cgccggcaac gctgccggtc aaactgtacc cgtccaacga
     1141 tcaggccagc cacaccggca ttgtcagcgg cagcgtcacc aacatgatga ccggcaaggg
     1201 tcgcttcgtg gtcaactacc tgggtgaggt cctcggcggt gaagcgacgc gcgtatcgaa
     1261 cgatgaaaga cgcggcgtcg cgagcgcatt cagtccgggc ggcatgtaca tgtcttgcga
     1321 gtatcagatg aacaccccct atcagggtgc cggtacgtgt tcgttcgcca acggcgccag
     1381 gtaccagatg cacatcggca attgatcagg atcctggttt gcgttgtcgc caggcgttga
     1441 tcaggtaaag gcgccacgct gtcctggtca ccatatagcc aaggactgca aggctggagg
     1501 cgagcgcgac cagacccacc gccaacggtt cggcaacctc gagcatccac atctgcatgg
     1561 cctgagccca ggcagcaacg gctgtggcgc tgaactctgg cggcaacagg aagccactgt
     1621 catcgccggt gagctgccgg ccaatctggt acgccagcag gtagagcggc acgatcgtga
     1681 atggattgct gtagagcgtg acgagcaagg ccagcggcag gttgactcgc aagagcagcg
     1741 caccgagcgc cgcccccaac atttgcaacg gccccggaat caacccgcaa aacaatccga
     1801 ccgccacggc gccggaaacc gagtggcggt tgaggtgcca taaccgggga tgcagcaata
     1861 cggcctgaaa gggacgcagc cagcgattcc ggagaatgct ttcgcgatcg gggaggtagt
     1921 ttttcaggag acggcgcatc gatgcattat cctgacaatt gcgccttgat gcgcccgaca
     1981 ccgcctccgg ccgccggctg gcgcactgct tatccccctt tacttccctt tcttcttggc
     2041 acccttgtcg gctgcagcgg acttgtcggc catcttctcg gccatcgccg tgctggaatt
     2101 catccgaccg ttgtcg
//
LOCUS       DAOSIT010000429         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_45266_length_2114_cov_9.969403, whole genome shotgun sequence.
ACCESSION   DAOSIT010000429 DAOSIT010000000
VERSION     DAOSIT010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45266_length_2114_cov_9.969403"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="PLL14_09565"
     CDS             complement(<1..113)
                     /locus_tag="PLL14_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOG04218.1"
                     /translation="MGGLDKGLQDLGGRPMIAWVIERLGPQVDELLINANQ"
     gene            complement(220..1128)
                     /locus_tag="PLL14_09570"
     CDS             complement(220..1128)
                     /locus_tag="PLL14_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOG04219.1"
                     /translation="MGKWSARVERAVAGLWPGKSGRKHAGGGSVNPAAASYVVECGSA
                     TDIGLVRNSNQDAVAFIVPSDPAQRLCRGVLAVVADGMGGHNAGEVASGLAIETICRS
                     YYATPGDDPRRALEVALREANDAVFRAAQADAALDGMGTTATALVLVGGGGWFAHVGD
                     SRLYRCSAGLCVQLTQDQTLVAELVKNQLLSAEQARHHPARNVLVSSLGTQPDLRVVA
                     ERCAPPLTGDCFVLCSDGLWESVEPGDIAEIVTSRGVEDACRDLVALALERDGHDNVS
                     VVALALRPAEPTPQAAAPRRATKHGE"
     gene            complement(1273..1854)
                     /locus_tag="PLL14_09575"
     CDS             complement(1273..1854)
                     /locus_tag="PLL14_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOG04220.1"
                     /translation="MKVFAAVMVSLSLASAVQLAWAQEEAALLADTRKTALPVLPKVV
                     QAMQTAVSEQGPVGAIPVCKEKAPQLLQDMRRQTGWNIRRVSLKSRNPATGTPDAWET
                     RQLAGFNIQFANGAKPEQLEVGEVVTAPDGKRSYRYMKALPVVEVCLSCHGTVDSLSA
                     DLRTALARDYPHDRATGYATGQVRGALSVIRPL"
     gene            complement(1923..>2114)
                     /locus_tag="PLL14_09580"
     CDS             complement(1923..>2114)
                     /locus_tag="PLL14_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG04221.1"
                     /translation="ILAIANDDRKLIFQGVQRVVGFDYGDLWEALEERRSRIVLIGKN
                     LPEKPLREAFLNCLQASLL"
BASE COUNT          400 a          739 c          604 g          371 t
ORIGIN      
        1 ttctgattgg cgttgatcaa cagttcgtcg acctgcggac cgagccgctc gatcacccag
       61 gcaatcatcg gtcggccacc gagatcctgc agacctttgt cgagaccacc catgcgccgg
      121 ccgagcccgc ccgcgaggac gacccccgtt attccatcga cgcgcaaggc gcatgcctct
      181 cgccgcgcgc gccgccgacc ctcagacact gagcgagggt cattcgccgt gcttggtggc
      241 gcggcgtggc gccgctgcct gtggcgtggg ctctgccggt cgcagggcaa gagccaccac
      301 ggaaacattg tcgtggccgt cgcgttcaag ggccagcgcc accagatccc gacaggcatc
      361 ttcgaccccc cgggaagtca caatttcggc gatgtcaccc ggctcgacac tctcccacag
      421 cccgtcggag cacaacacga agcagtcgcc ggtcaagggc ggcgcgcaac gctcggccac
      481 cacccgcagg tcgggctgcg tgcccaggga cgacaccagc acattgcgtg cgggatgatg
      541 acgcgcctgt tcagcactca gcaactggtt cttcaccagt tccgccacca gcgtctgatc
      601 ctgcgtcaat tgcacgcata gccccgccga gcaacggtag aggcggctgt caccgacatg
      661 cgcaaaccac ccgccgccgc cgaccaggac cagggccgtc gctgtcgtgc ccatgccgtc
      721 cagcgcggca tcggcctgcg cggcacgaaa aaccgcgtca ttcgcctcgc gcaacgccac
      781 ttccagcgca cgccgggggt catcacctgg cgttgcataa tagctgcggc aaatcgtctc
      841 gatcgccagc ccactcgcca cttcaccggc gttgtgcccg cccatgccat ccgccacaac
      901 cgcgagcacc ccgcgacaca gccgttgtgc aggatcactc gggacaatga aagccaccgc
      961 gtcctgattg ctgtttcgca ccaggccgat atcggtcgcc gaaccgcact cgacgacata
     1021 acttgccgcc gcgggattca ccgacccccc tccggcgtgt ttgcggccag atttgccagg
     1081 ccagaggccc gccaccgcgc gctccacgcg cgcactccac ttgcccatat tccctgcccc
     1141 ttttcgggca tccccgcgcc acgttttgcc attgaaaccg ctctttcgga tacagtcgcc
     1201 attgtgccag tctgcgctcg tcgaaggggg gtatgcgcga agcgcttgcg tctcagtccg
     1261 gcgcaaaccc gctcaaaggg gccgaatgac cgacaacgcg ccgcgtacct gcccggtggc
     1321 ataacccgtt gcacgatcat gcgggtagtc tctggccagg gccgtcctga gatcagccga
     1381 cagcgaatcg acggtaccgt ggcagctcag gcagacctcg actaccggca aggctttcat
     1441 gtagcgatag ctacgcttgc catcgggggc ggtaaccacc tcgccaactt cgagctgctc
     1501 gggcttggcg ccattggcaa actgaatgtt gaagccagcg agctggcgcg tttcccaagc
     1561 atcgggcgtg ccggtcgctg gattgcgtga cttcaggctg acgcggcgaa tattccagcc
     1621 cgtctgccga cgcatgtcct gcagcagttg tggcgctttc tccttgcaga cgggaatcgc
     1681 accgaccgga ccttgttcgc tgactgcggt ctgcatcgcc tggacaacct tgggcagcac
     1741 cggcagcgcg gtcttgcgcg tgtcggcaag caaggcagct tcctcctgcg cccacgccag
     1801 ctgaacggcg gaagcaagcg acagggaaac catgactgct gcgaaaacct tcatgtggac
     1861 ctccgataat gtataagcat gcgctaattt accattatct cgtaccaacc gtcacgaaac
     1921 catcacaaca aagaagcttg caggcagttc agaaaggctt cgcgcagcgg cttttcgggg
     1981 agattcttgc caatcaggac aattcttgaa cggcgttcct ccagcgcctc ccaaaggtcg
     2041 ccgtaatcga agccgacgac gcgctggaca ccctggaaga tcagtttccg gtcgtcgttg
     2101 gcgatcgcca ggat
//
LOCUS       DAOSIT010000430         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_45350_length_2111_cov_10.611868, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000430 DAOSIT010000000
VERSION     DAOSIT010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45350_length_2111_cov_10.611868"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2111
                     /locus_tag="PLL14_09585"
     CDS             <1..>2111
                     /locus_tag="PLL14_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG04222.1"
                     /translation="ARIRHKYKIKNTTGYSLNALIDYEDPIDILAHLMIGSEGTLGFM
                     SRITYRTVVEDPCKASTLVFFPTIETACQAVIRLKQEPVSAVEMLDRPALRSVEDKPG
                     LPHIIRTLGDDAAALLIEVRADSAALLQQRIDAALSALTGIATVEAPTFSTDPATCEM
                     YWKVRKGTFPSVGAMRRAGTTVIIEDVAFPIESLAAATLDLQALLRQHGYSEGIIFGH
                     ALEGNLHFVFTQDFSDAAEVERYARFMDDVCKLVIDKYDGSLKAEHGTGRNMAPFVEM
                     EWGRQATDLMRRIKTLFDPDNRLNPGVIINDDPQAHLKNLKPMPAAEDIIDRCIECGF
                     CEPLCPSHRLTLSPRQRIVSWRELSRRAAAGEEAADVGKDFAYFGLDTCAGCGLCSTA
                     CPVGIDTGELTRRLRGRGLGNKAPSHQVAHWTAENFGKVATASRVGLSIGHAVSSVVG
                     ESLVARLSRGAWKRGMPQAGRAPKPHRGTGAGDPVVYFPACGGRIFGANTPDEATLPE
                     VVIELLLRAGYDPRLPEGFDKLCCGQMLASKGMAEEAEQMAESVTAALLRAADDGHGG
                     HYPVIMDASTCSVRMQKHLAGRLTVLDFHEFAHDALLPRLMVTRKRGPIALHINCSVR
                     RVASDNKLRRLIAACAEEIVEPAGVTCCGFAGDRGFVVPELNVHALRKVHAALPANCC
                     EGVSTNRTCEIGLTAETGRPYR"
BASE COUNT          374 a          747 c          645 g          345 t
ORIGIN      
        1 gcgcgcatcc gccacaagta caagatcaag aacaccaccg gctacagcct caatgccctg
       61 atcgactacg aagacccgat cgatattctt gcccacctga tgatcggctc ggaggggacg
      121 ctcggcttca tgtcacgcat cacctaccgc accgtcgtcg aggacccttg caaggcgagc
      181 acactggtgt tcttccccac catcgagacc gcctgccaag ccgtcatccg cctcaagcag
      241 gagccggtgt ccgccgttga aatgctcgat cgtccggccc tgcgctcggt ggaagacaag
      301 cccggtctgc cgcacatcat tcggacgctg ggcgacgatg ccgctgcgct gctgatcgag
      361 gtgcgcgcgg acagcgccgc gctcctgcaa cagcgcattg acgccgcgct cagtgccttg
      421 accggcatcg ccaccgttga agcgccgacg ttctccaccg atcccgccac ctgcgaaatg
      481 tactggaaag tgcgcaaggg cactttcccc tcggtcggcg cgatgcgtcg cgccggcacc
      541 acggtgatca tcgaggacgt ggccttcccg atcgagtccc tggccgccgc cacgctcgac
      601 ctgcaggcgc tgttacgcca gcacggctac agcgaaggaa tcatcttcgg gcatgcgctc
      661 gaaggcaatc tgcatttcgt gttcacccag gatttctcgg atgcggcgga ggtggaacgt
      721 tacgcccgct tcatggacga tgtctgcaaa ctggtcatcg acaagtacga cggttcgctc
      781 aaggccgaac acgggacagg ccgcaacatg gcaccctttg tcgagatgga atggggccgg
      841 caggccaccg acctgatgcg ccggatcaag acgctcttcg atcccgacaa ccggctgaat
      901 cccggagtga tcatcaacga cgacccgcag gcgcacctga agaacctcaa gccgatgccg
      961 gccgccgaag acatcatcga ccgctgcatc gaatgcggct tttgcgagcc tctgtgcccc
     1021 tcgcaccgcc tgaccctgtc gccgcgccag cgcatcgtca gctggcgcga actgtcgcgc
     1081 cgcgccgccg ctggcgaaga ggccgccgac gtcggcaagg attttgccta ctttggtctc
     1141 gacacctgcg ccggctgcgg cctttgttcc accgcctgtc cggtcggcat cgacaccggc
     1201 gagctgacgc gccgcctgcg cggccgcggg ctgggcaaca aggcgccttc gcatcaggtc
     1261 gcgcactgga cggcggagaa tttcggcaag gtagccaccg cctcccgcgt cggcctgtcc
     1321 atcggccatg ctgtctccag cgtggtcggc gaaagcctcg tcgctcgcct ctcgcgtggc
     1381 gcctggaagc ggggaatgcc acaggcgggc agggcgccca aaccccacag aggcaccggt
     1441 gcgggcgacc cggtcgtgta tttccccgcc tgtggtggcc gcatctttgg cgccaacacg
     1501 cccgacgagg cgaccctgcc ggaagtggtc atcgagctgc tgctgcgtgc cggctacgac
     1561 ccgcgcctgc cggaaggctt cgacaagctg tgctgcggtc agatgctggc gagcaagggc
     1621 atggccgaag aggccgaaca gatggccgag agcgtaaccg ctgcgctgct cagggcggcg
     1681 gacgacgggc atggcggcca ctatccggtg atcatggatg ccagcacctg ctcggtacgc
     1741 atgcagaagc acctcgccgg tcgcctgacg gtgctcgatt tccatgaatt cgcccacgat
     1801 gcgctgctgc cgcgcctgat ggtcacgcgc aagcgaggac ccattgccct gcatatcaac
     1861 tgcagcgtcc gccgcgtcgc ctccgacaac aagctgcgtc gcctgatcgc ggcctgcgcc
     1921 gaggaaatcg tcgaacccgc cggcgtcacc tgctgcggtt tcgccggcga ccgcggtttc
     1981 gttgtcccgg aactcaacgt gcacgccctg cgcaaggtac acgcggcact gcccgccaac
     2041 tgctgcgagg gagtctcgac caaccgcacc tgcgaaatcg gcctcaccgc cgaaaccggc
     2101 cgcccctacc g
//
LOCUS       DAOSIT010000431         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_45360_length_2111_cov_5.340953, whole genome shotgun sequence.
ACCESSION   DAOSIT010000431 DAOSIT010000000
VERSION     DAOSIT010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45360_length_2111_cov_5.340953"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLL14_09590"
     CDS             complement(<1..521)
                     /locus_tag="PLL14_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04223.1"
                     /translation="MAPQPADELPLLRAHLKALREIDGTLPQRARLLDGLYSRSTMVT
                     SRLLPSLSVDLVLPVPRKTRRIVRSLLDLLQMLAEDTLALLDELDRQHAPDKERQLAL
                     WRSVYVLAQQLMISDLIASPARAGTWLLLHQTYAEARRLQLHATLPRDEVHSLQHLYH
                     AAVLLGWAQPASLT"
     gene            954..1208
                     /gene="rpsP"
                     /locus_tag="PLL14_09595"
     CDS             954..1208
                     /gene="rpsP"
                     /locus_tag="PLL14_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG04224.1"
                     /translation="MVVIRLARGGAKKRPFYNMVVADSRARRDGRFIERVGFYNPLAA
                     GQEQGLYVAVDRLNYWQQQGAQTSPTVARLLKQSAAKVAA"
     gene            1212..1772
                     /gene="rimM"
                     /locus_tag="PLL14_09600"
     CDS             1212..1772
                     /gene="rimM"
                     /locus_tag="PLL14_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768513.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG04225.1"
                     /translation="MPDTTGSAVPGLDAPPADIVVLGKVMAACGLQGEVRVFPFADDP
                     LGWSQLPIWWIGREGDAPHLWHRTRLLTCRLRNDLLVARLACVTDRTAAEAMRGVLVG
                     VPRAALPPTATGEYYWADLLGLTVLNTREQSLGRILGLIETPANTVLRVGDGEGAERL
                     LPFVATVVLDVDLAARQVRVEWEMDW"
     gene            1841..>2111
                     /locus_tag="PLL14_09605"
     CDS             1841..>2111
                     /locus_tag="PLL14_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG04226.1"
                     /translation="MFVALTQSGVTRKALNSGRWSLRLWNPRDFTSDRHRTVDDRPYG
                     GGPGMVMIPQPLEAAIKAAKARQQALGAASRVICLSPQGPLLTHRR"
BASE COUNT          363 a          630 c          681 g          437 t
ORIGIN      
        1 gtcaacgacg ccggctgtgc ccagccaagc aggactgcgg cgtgatagag atgctgcagg
       61 ctatgaacct cgtccctggg aagcgtcgcg tgcaactgca gcctgcgtgc ctccgcatag
      121 gtctgatgca gcagcagcca ggtgcctgcg cgcgcgggcg aggcaatcaa gtcactgatc
      181 atcaattgct gcgccagcac atacacgctg cgccacagtg ccagttgacg ctccttatct
      241 ggggcgtgtt gccgatccag ttcatcgagc agcgcaagcg tgtcttcagc aagcatctgc
      301 agcaagtcca gaaggctgcg gacgatgcgg cgcgttttcc gcggcaccgg caataccaga
      361 tcgacgctca gcgaaggtag cagtcgggaa gtgaccatcg tgctacggga gtacaagccg
      421 tccagtaggc gcgcgcgctg cggcagcgtt ccgtcaatct ccctcaaggc cttcagatgt
      481 gctcgcagca aggggagttc gtcggcgggc tgaggtgcca atggcatctc cagccactgc
      541 tggatgcttc ggatcccgga cggaagcgca gcggttcgtt ctgtaccagc ggcattgaat
      601 acggtgtcgg tcatcaggcc acctgccaaa gggggttcca ttgttgtctg tcagcgatgt
      661 cgctgggtac ctttcggctg cacagggcaa cttccgtgca gtctgggcgc aggtcataaa
      721 atcaatgcct tgaccgactg ccgccggtca taaaatcaat gccttgaccg actgccgccg
      781 gtctgccgat tatacctgtg agggcgcctg ctgggggttg tcaagtttcc accgttgtcc
      841 ggctcgatct tggccggcgg cccggattgt tctcgaacca gccaacaggt gcttcattat
      901 tcgccctttc ttgttataat cgccctcttt ttctggattc ggaagaaact atcatggttg
      961 ttattcgact ggcccgtggt ggcgcgaaaa agcgtccctt ctacaacatg gtggtggctg
     1021 attcgcgcgc tcgccgcgat ggccgtttca tcgaacgtgt tggcttctac aacccgctgg
     1081 cagcaggcca ggaacaaggc ctgtacgttg ccgtcgaccg cctgaactac tggcagcagc
     1141 aaggtgccca gacctcgccg accgttgctc gtctgctcaa gcagtccgct gccaaggtgg
     1201 cagcctgaac cttgcctgac acgacagggt ccgctgttcc ggggctcgac gcaccacccg
     1261 ccgatattgt tgtcctgggc aaggtcatgg ccgcctgtgg gctgcagggc gaggtgcggg
     1321 tttttccgtt tgccgatgat cccctcggct ggtcgcaatt gcccatctgg tggatcggtc
     1381 gcgagggtga tgcgccgcat ctctggcatc ggacaagact gctcacctgc aggctacgaa
     1441 atgacctgct ggttgcccgt ctggcgtgtg tgaccgaccg taccgctgcg gaagccatgc
     1501 gcggtgttct ggtgggcgta ccgcgggccg cgttgccgcc gaccgctacg ggtgaatact
     1561 actgggccga cctgcttggc ctgacggtgc tcaacactcg ggagcagtcc ctggggcgga
     1621 ttctcggcct gatcgaaacc cctgccaata ccgttctgcg ggttggcgat ggcgagggcg
     1681 cagaaaggct gctgcctttt gtggcgacgg tcgtgctgga tgtggacctg gcagcaaggc
     1741 aggttcgtgt cgaatgggaa atggattggt gattgcgtcg gagccggtgc cgccagcagc
     1801 ggcgccgttc gtcgccgatg tcgtgacctt gtttcccgag atgtttgtcg cgctgacgca
     1861 gtcgggcgtg acccgcaagg ctctgaactc cggccgctgg agcctgcggc tgtggaatcc
     1921 gcgcgatttc accagcgacc ggcatcgcac ggtcgatgat cgtccgtacg gcggtgggcc
     1981 agggatggtg atgataccgc agcctctgga agcggcgatc aaggcggcaa aagcgcggca
     2041 acaagcgctt ggcgcagcca gccgcgtcat ctgcctgtca ccgcaggggc cactgctgac
     2101 gcatcggcgg g
//
LOCUS       DAOSIT010000432         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_45567_length_2104_cov_6.375305, whole genome shotgun sequence.
ACCESSION   DAOSIT010000432 DAOSIT010000000
VERSION     DAOSIT010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45567_length_2104_cov_6.375305"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLL14_09610"
     CDS             <1..968
                     /locus_tag="PLL14_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG04227.1"
                     /translation="LACHRHAFEWFNGVPRKVRIDNPKCAITRACYYEPTVQRAYAEL
                     ALGYAFVIDPCPVADPAKKGRVESGVKYVKGNFMPLREFHSLAHANAQLEAWIQGEAG
                     NRLHGSTRERPLMRFNETEQALLQPLPAVAPTCPTWAKAKLHGNGHVQHAYCHYSAPF
                     RLIHQTLWLEITPDVVRLYHEHELVAIHPRLFKAGTRSTVADHLPPDAQAYFMRDPQW
                     CLTQATAVGPACRAVVESLFAKRVLDQLRAVQGLLRLADQFGRNRLDAACSRALNFGT
                     PSYRTIKQILQTGLDQQPELIDAPTLEAPYLSGGHFRRQPSDLLH"
     gene            1024..1827
                     /gene="istB"
                     /locus_tag="PLL14_09615"
     CDS             1024..1827
                     /gene="istB"
                     /locus_tag="PLL14_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOG04228.1"
                     /translation="MNPMLPLEPLLKQLRLSGILDSLEARNRQAIEAKLAYTDFLALL
                     VEDEVARRDQRQFAQRLRKAQVVGDKTLERFDCSHSPSVNHALIAELATCRFVLEQAP
                     VLIAGPTGTGKSHLVQSLCHCALRAGHEVLFISHAKLLAQLHAARATDSFERKLRQLA
                     RVDVLAIDDFGLRPMRSPQDEDFHDLIDARYERTTTLLTSNLHFDEWGAAFPNKLMGA
                     ATLDRLRDRAYQIILDGETRRKPRPLPEVRSPVQATAGRDASKPPPAAR"
BASE COUNT          382 a          721 c          623 g          378 t
ORIGIN      
        1 ggctggcctg ccaccggcac gccttcgagt ggttcaacgg cgtcccgcgc aaggtacgga
       61 tagacaaccc gaagtgcgcg atcacgcgcg cctgctacta cgagccgacg gtgcagcgcg
      121 cctacgccga actcgcgctc ggctatgcct ttgtaatcga cccctgtccg gtcgcggacc
      181 cggccaagaa aggccgcgtg gagtcgggag tcaaatatgt caagggcaac ttcatgccgc
      241 tgcgcgagtt tcatagcctg gcgcacgcga atgcgcaatt agaggcgtgg attcagggcg
      301 aggcaggcaa tcgcctccac ggcagtacgc gtgagcggcc gctcatgcgc ttcaacgaaa
      361 ccgagcaagc gctgctgcag ccgctgcccg ccgtcgcgcc aacgtgccca acctgggcta
      421 aggccaagct gcacggcaac ggccacgtcc agcatgcgta ttgccactac tcagcgccgt
      481 ttcgcctgat ccaccagacg ctgtggttgg aaatcacgcc ggatgtcgta cgcctctatc
      541 acgagcacga gctggtggcg atccatccgc gcctgttcaa agcgggtacg cggtcgaccg
      601 tcgcggatca tctgccaccc gacgcccagg cttacttcat gcgcgatccg cagtggtgct
      661 tgacccaggc cacggcggtc gggccggctt gtcgggcggt cgtcgagagc ttgttcgcga
      721 agcgtgtgct cgatcagctg cgcgccgtcc agggcttgct gcgcttggcc gatcagttcg
      781 gtcgcaaccg actcgacgcg gcgtgtagcc gcgcgctgaa ctttggtacg ccgagctatc
      841 gcacgatcaa gcagatcctc cagaccggac tcgaccagca gccggagttg atcgacgcgc
      901 cgacgctgga agcgccctat ctgagtggcg gccacttccg ccgccaaccc tccgatctgt
      961 tgcattgagc cgccgcacgg cgcgccgctc cttgctcatc ccttatctat ctggaatcct
     1021 ctcatgaatc ccatgctccc acttgaaccc ctgctcaagc aactgcgcct gtccggcatc
     1081 ctcgactcgc tcgaggcgag aaaccgtcag gcgatcgagg ccaagctggc ctacaccgac
     1141 ttcctggcgt tgctggtcga ggacgaagtc gcccgtcgtg atcagcgtca gtttgcccag
     1201 cgcctgcgca aggcgcaggt ggtcggcgac aagactctgg aacgtttcga ttgcagccac
     1261 agtccgtcgg tcaatcacgc cttgattgcc gagctggcaa cctgccgatt cgtcctcgaa
     1321 caggcgccgg ttctgatcgc cggtccgacc ggtacgggca agtctcacct ggtccagtcg
     1381 ctctgccatt gcgcactgcg cgccggccat gaggtcctgt tcatttcgca cgccaagcta
     1441 ctggctcagc tgcacgcggc gcgggcgacc gattcctttg agcgcaagct gcgtcaactg
     1501 gcccgcgtcg atgtgctggc aattgatgac ttcgggctgc gtcctatgcg ctcgccgcag
     1561 gatgaggatt tccacgatct gattgatgcc cgttatgagc gcacgaccac cttgctgacc
     1621 agcaatttgc acttcgacga gtggggcgcg gcgtttccca acaagttgat gggggcggcg
     1681 acgctcgacc gtttgcgcga ccgtgcttat cagatcattc tcgacggcga gacccggcgc
     1741 aaaccgcggc ccctgccgga agtccggtcg cccgtccagg ccacggcggg cagggacgct
     1801 tccaagcctc ccccggccgc acgttagacc ctcagcggcg agcaaaccct ggggcgctgc
     1861 cccagacccc gcactcgccg tgaggcagcc gccggggatg aaaaaacatc cccgacaact
     1921 gccgtcagcg tctgcgacct ccagggtgtc aaggggcttt cgcggcataa acgccaaaca
     1981 gccggcattt attccacggt cccctggaca cccttccggt cgccacttgc cacgttgcaa
     2041 aaagactgaa aacggcgtaa aaccgacccg tccgaaaccg ctaaaacagc cacttctgct
     2101 ggcc
//
LOCUS       DAOSIT010000433         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46045_length_2089_cov_10.245329, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000433 DAOSIT010000000
VERSION     DAOSIT010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46045_length_2089_cov_10.245329"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLL14_09620"
     CDS             <1..288
                     /locus_tag="PLL14_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HOG04229.1"
                     /translation="VPFFGVQAATITGLSRLARLAGAAVVPCVTRILPNAEGYIVTVG
                     EPWQDFPSEDVEADTRRMNAWIETAVRTMPEQYYWVHRRFKTRPPGEPRPY"
     gene            435..>2089
                     /locus_tag="PLL14_09625"
     CDS             435..>2089
                     /locus_tag="PLL14_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014851331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOG04230.1"
                     /translation="MALTLLGLSGALTHDPSAALYIDGRLVAAAEEERFVRDKHAKNR
                     MPYEAAKFCLDFAGIKPADVDAVAIPFAPISLSEPARWHYAKRYWYAPERAMDAILSG
                     NRRYYRYRKRIEWCLQQLGFDLKNTEIVPVEHHLAHAASAYHCSGFREKTAILGIDGK
                     GEYATTFFGYGENGRIHKLKEFYDPDSLGGLYGALTEYLGFEMLDGEYKVMGMAPYGD
                     PGKYDFSRLAKFENGELTINTDYANVIGLRRYKESGKGYYFSPKLVDWLGPRRSGDVA
                     DDPYIHYAASMQKLFEDLSLQMIDYYLADILRDTGRLAFSGGGALNVKLNQKIIARPD
                     VRELFVQPASGDAGTAVGAAAYVSVQRGVAVEKMEHVYLGPAYSNEDVIAACARHPAQ
                     PAWQRIEQVPQRVARLLADGHPVAWFQGRMEFGPRALGGRSILGCPSVAGVADRINAQ
                     IKFRERWRPFCPSMLDSVGPQMLNSGHPAPFMTFTFEVAPGWKERVPEVVHEDGTSRA
                     QVLKREFNPRYHDLMSELEKLTGNGVVLNTSLNRRGEPMICSPT"
BASE COUNT          388 a          651 c          643 g          407 t
ORIGIN      
        1 gtcccgtttt ttggcgtgca ggcggccacc atcaccggtt tgtcgcgttt ggcgcggctt
       61 gcaggcgccg ccgtcgtccc ctgcgtgacg cgtatcctcc ccaacgccga gggctacatc
      121 gtaactgtcg gagagccatg gcaagatttt ccctccgagg acgtcgaagc cgataccagg
      181 cgcatgaatg cctggatcga aacggcggta cgtacgatgc cggagcagta ttactgggtg
      241 caccgtcgtt tcaagacccg gccgcccggt gaaccgcgtc cctattgatc cttgcggcgt
      301 ttcgccggcc ggaccggctt gttccgacga gcgaacagca gatgggccgg ggcaggtata
      361 cttgccgatt tcgtcgaccc gggaccgctg atgcacgttc tgggtcgaag gtgtgatcct
      421 ctgagacctc gaccttggca ctgaccctac ttggattgtc cggcgcgctg acccacgacc
      481 cttcggcggc gctttacatc gacggcaggc tcgtcgcagc cgcagaagag gaacgcttcg
      541 tccgcgacaa gcacgccaag aaccgcatgc cctacgaagc cgcgaagttc tgcctcgact
      601 ttgccggcat caagcccgcc gatgtcgacg ccgttgccat cccctttgcc ccgatcagcc
      661 tttccgagcc cgcgcgctgg cactatgcca aacgctactg gtacgcgccg gagcgtgcca
      721 tggacgccat tctgagcggt aaccggcgct attaccgtta ccggaagcgt atcgaatggt
      781 gcctgcagca actcggtttc gatttgaaaa atacggaaat cgtgccggtc gagcatcatc
      841 tggcgcacgc ggccagtgcc taccattgct cgggattccg ggagaagacg gcgattctcg
      901 gcatcgatgg caagggcgag tacgccacca ctttttttgg ctacggcgag aatggccgga
      961 ttcacaagct caaggagttc tacgatcccg attccctggg cggtttgtac ggcgccttga
     1021 ccgagtatct gggtttcgag atgctggacg gcgagtacaa ggtgatgggc atggcgccct
     1081 atggcgaccc cggtaaatac gacttctcgc gcctggccaa attcgagaac ggtgaattga
     1141 ccatcaacac cgactatgcc aacgttatcg gcctgcgtcg ctacaaggaa agcgggaaag
     1201 gctattattt ttcaccgaaa ctggtcgact ggctgggtcc gcggcgttcg ggggacgtcg
     1261 ccgacgaccc ctacatccac tacgcggcaa gcatgcagaa gctgttcgag gatctgtcgt
     1321 tgcagatgat cgactattac cttgccgaca ttctgcgcga caccggacgc ctcgcctttt
     1381 cagggggggg cgcgttgaac gtgaagctca accagaaaat catcgcccgt cccgatgtca
     1441 gggaactctt tgtccagccc gcttccggcg atgccgggac ggcggtcggc gcagccgctt
     1501 atgtctcggt gcagcgcggc gtggcggtcg agaagatgga gcacgtatac cttgggcctg
     1561 cgtacagcaa cgaagacgtg atcgccgcct gcgcccgcca tccggcgcag cctgcgtggc
     1621 aacggatcga gcaggtgccg cagcgcgtcg ccaggttgct ggcggacgga caccccgtgg
     1681 cgtggtttca ggggcggatg gaatttggtc cgcgcgccct tggcggccgc tcgattctgg
     1741 gctgcccgag cgttgccggg gtcgccgacc ggatcaacgc ccagatcaag ttccgcgagc
     1801 gctggcgacc gttctgcccg agcatgctcg acagcgtcgg gccgcagatg ctgaacagcg
     1861 gacatcccgc accgttcatg accttcacct tcgaagttgc cccaggctgg aaggaacgtg
     1921 tccccgaagt cgttcacgaa gacggtacct cgcgtgcgca ggtgttgaag cgcgagttca
     1981 acccgcgtta tcacgacttg atgagcgaac tcgagaagct gaccggtaac ggcgtggtcc
     2041 tcaacacttc gctcaatcgc cgcggcgaac cgatgatctg ctctccgac
//
LOCUS       DAOSIT010000434         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46257_length_2082_cov_6.210163, whole genome shotgun sequence.
ACCESSION   DAOSIT010000434 DAOSIT010000000
VERSION     DAOSIT010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46257_length_2082_cov_6.210163"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLL14_09630"
     CDS             <1..575
                     /locus_tag="PLL14_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOG04231.1"
                     /translation="SATNGFGMGLATAAVMAASSLLVATFRNHISQEVRIPVFILIVA
                     TMVTLVDLAMNAWMHELYKVLGLFIPLIVSNCLPLARLEAFAAKAPVVPAFVDGLCMG
                     LGFTIALTTIGAVREIIGSGTLFADASLLLGPSFKFMELRLLPASMGVLMMILPPGGF
                     LVTGLMVVCKRLLDLRAGKEIQMAGAHGIA"
     gene            597..1091
                     /locus_tag="PLL14_09635"
     CDS             597..1091
                     /locus_tag="PLL14_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG04232.1"
                     /translation="MAATGSSGDTVEGIARVVHTKDGVIWLEPEQTTSCGACAAAGQC
                     GSKGIGSLASRIEARRFVLHGQQDFGVGERIVIGVGAQTLLKGALIAYGLPLASSLIA
                     GGAVQAFGGGDVESMAGAAAGLAVGLLLAGFGARRLSARGELAPRFLRRAQPGESCSG
                     KDEA"
     gene            1088..1669
                     /gene="rsxA"
                     /locus_tag="PLL14_09640"
     CDS             1088..1669
                     /gene="rsxA"
                     /locus_tag="PLL14_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG04233.1"
                     /translation="MKDYALMMIGAALVNNVILARFLGLCSFMGVTTRIDAAAGMGLA
                     TTFVITVSSMADWAVQHYLLAPYGLGFLRTVTFILVIASAVQFTELTVRKVSPQLFQM
                     LGIYLPLITTNCAVLGIALLLVEGRMSFMTSTLFAFASSVGYSLVMVIFAGQRERLAV
                     AAVPRLFAGPPIGFIVASLLAMAFMGFSGISTG"
     gene            1683..>2082
                     /locus_tag="PLL14_09645"
     CDS             1683..>2082
                     /locus_tag="PLL14_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOG04234.1"
                     /translation="MFIAVASLTLMGIALGGLLGLAARYLAVEENPLEAELQALLPGS
                     QCGQCGFVGCAQAAAALARNEAAPTLCPPGGRAVAEAIAARLGIRIDLAAVRDDGPQL
                     AFVSEDICTGCCRCSKVCPTDAIIGAAKQIH"
BASE COUNT          303 a          636 c          710 g          433 t
ORIGIN      
        1 ccagtgccac caacggcttc ggcatggggc tggcaacggc ggcggtgatg gcggcctcca
       61 gtctgctggt cgccaccttc cgcaaccaca tcagccagga ggtacggatc ccggtgttca
      121 tcctcatcgt ggccacgatg gtgactctgg ttgacctggc gatgaacgcc tggatgcacg
      181 agttgtacaa ggtgctgggt ctgttcattc cgctgatcgt ctcgaactgc ctgccgcttg
      241 cgcgtctgga agctttcgcc gccaaggcac cggtcgtgcc ggcattcgtc gatggcctgt
      301 gcatgggcct tggcttcacg atcgcgctga ccaccatcgg cgcggtgcgc gagatcatcg
      361 gctcgggtac gcttttcgcg gatgcctcgc tgcttctcgg accgtccttc aagttcatgg
      421 aactgcgcct gttgccggcc agtatgggag tgctgatgat gatcctgccg cccggaggtt
      481 ttctggtgac agggctgatg gtggtgtgca agcgcctgct ggatctgcgt gccggcaagg
      541 aaatccagat ggccggcgcg cacgggatcg cctgaatcat gacgaagggg atcagcatgg
      601 cagcaaccgg cagcagtggc gacacggtcg agggcattgc ccgcgtcgtt cacacgaagg
      661 atggcgtgat ctggctggaa cccgagcaga ctaccagttg tggcgcctgc gccgcggctg
      721 ggcagtgtgg cagcaagggt atcggcagcc ttgccagccg tatcgaggcg cgacgctttg
      781 tgctgcacgg gcagcaggat ttcggcgtcg gtgagcgcat cgtgatcggc gtcggcgcgc
      841 agaccctgct caagggcgca ttgatcgctt acggcttgcc gttggccagt tcgctgatcg
      901 ccggcggcgc cgtgcaggcg tttggcggtg gcgatgtcga gagcatggcc ggcgcggcgg
      961 cgggtctggc agtcggcctg ctgctggcgg gtttcggggc ccggcggctt tctgcacgtg
     1021 gtgaattggc gccccgcttc ctgcgccggg cgcagcctgg cgagtcttgc agtggaaagg
     1081 acgaggcatg aaagactacg cactgatgat gatcggcgcg gcgctggtca acaacgtgat
     1141 cctggcgcgc tttctgggcc tgtgctcgtt catgggcgtc acgacacgga tcgacgcggc
     1201 cgccggcatg gggttggcga cgaccttcgt cattaccgtg tcgtcgatgg ccgactgggc
     1261 ggtgcagcac tacctgctgg cgccctacgg cctgggtttc ctgcgcaccg tcaccttcat
     1321 cctggtgatt gccagcgcgg tgcagttcac cgaactgacg gtacgcaagg tctcgccgca
     1381 gttgttccag atgttgggca tctacctgcc gctgatcacc accaactgtg cggtgctcgg
     1441 cattgccctg ctgctggtcg aaggtcgcat gagcttcatg accagcacgc tgttcgcctt
     1501 cgcctcgtcg gtcgggtaca gcctggtgat ggtgattttt gccgggcagc gcgaacgcct
     1561 ggctgtggcc gcggtgccgc gactttttgc cgggccgccg atcggtttca tcgttgccag
     1621 cctgctggcg atggctttca tgggtttttc cggtatcagc accggttgaa aagggggtcg
     1681 tcatgttcat cgcagtcgcc agtctaaccc tcatgggtat tgccctcggc ggtcttctcg
     1741 gactggccgc ccgttacctc gcggtcgagg agaatccgct cgaagccgaa ttgcaggcgc
     1801 tgttgcccgg ctcgcaatgt gggcagtgcg gttttgtcgg ctgcgcccag gccgcggcag
     1861 ccctggcgcg caatgaagcg gcaccgacgc tctgcccgcc gggcggacgc gctgtcgcgg
     1921 aagccatcgc cgccagactc ggtatccgga tcgacctcgc ggcggtacgt gacgatggtc
     1981 cgcaactcgc ctttgtctcc gaggacatct gcaccggctg ttgtcgttgc agcaaggtct
     2041 gcccgaccga cgcgatcatc ggcgcggcca agcagatcca ca
//
LOCUS       DAOSIT010000435         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46373_length_2078_cov_9.626792, whole genome shotgun sequence.
ACCESSION   DAOSIT010000435 DAOSIT010000000
VERSION     DAOSIT010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46373_length_2078_cov_9.626792"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PLL14_09650"
     CDS             <1..1417
                     /locus_tag="PLL14_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOG04235.1"
                     /translation="DIGKNIVGVVLGCNGYDVIDLGVMVSCDRILHAAREHGAQAVGL
                     SGLITPSLEEMSHVAAEMERLGFAAGADGGPPVPLLIGGATTSRAHTAIKIAPNYSGP
                     VVYVPDASRAVGVVTRLLSIDNAADFRVELAADHAKVRAQHANKKGIKLLTLPAVRAN
                     RFVWNGDAAYRPTAPKKPGVHVLREIDLATLVDYIDWGPFFQTWDLAGSYPKILDDAV
                     VGATARAVFNDARALLEKVVSEKWLRTNAVFGVFPANSVGDDIEIYADETRSQPSMVW
                     HNLRQQQERPAGKPHYCLSDFVADRGTPDWIGAFAVGAGFGIEEKLAEFNAAHDDYHA
                     IMLKAIADRFAEACAEWLHAQVRRDYWGYASDEDLDRAALIREDYRGIRPAPGYPACP
                     DHTAKGALFELLDAPRNTGMALTESFAMSPAAAVSGFYLAHPQARYFAVSKIARDQLE
                     DWAQRTGFSLSEAEHWLAPLL"
     gene            1507..>2078
                     /locus_tag="PLL14_09655"
     CDS             1507..>2078
                     /locus_tag="PLL14_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG04236.1"
                     /translation="MALPGQAACYAEIRAAAQLAQLAGPSGRRQFVLGGGSNLVLGGD
                     FDGLILHMAIAGRQLLGEDAEAWYVYAGAGENWHQLVLWTLQQGWPGLENLALIPGTV
                     GAAPIQNIGAYGLEVADRVLSLDAVDMLTGKTIRLDRADCRFAYRDSVFKQEGWHLDA
                     RRVVIGVTFRLPKAWRPLTGYGDLAAELDRR"
BASE COUNT          359 a          706 c          669 g          344 t
ORIGIN      
        1 cgacatcggc aagaacatcg tcggcgtggt gctcggctgc aacggctacg atgtcatcga
       61 cctcggggtc atggtttcct gcgacaggat tctgcacgca gcgcgtgagc acggcgcgca
      121 agcggtcggc ctgtcgggtc tgatcacgcc gtcgctggag gaaatgagcc acgtcgcagc
      181 cgagatggag cgtctcggct ttgccgccgg cgccgacggt ggcccgccgg ttcccctgct
      241 gatcggtgga gcgacgacca gccgtgcgca cacggcgatc aagatcgcgc cgaactattc
      301 cgggccggtg gtctatgtcc cggatgcctc gcgcgccgtt ggcgtcgtca cccgcctgtt
      361 gtcgatcgac aacgccgccg atttcagggt cgaactggcc gccgaccacg ccaaagtgcg
      421 tgcccagcac gccaacaaga aaggcatcaa gctattgacg ctgccggcag tgcgtgccaa
      481 ccgtttcgtc tggaacggcg atgccgccta tcgaccgacg gcaccgaaga aaccgggcgt
      541 gcatgtgctg cgcgagatcg atctcgccac cctggtcgac tatatcgact gggggccgtt
      601 ttttcagacc tgggatctcg ccggcagcta cccgaagatt ctcgacgatg ccgtcgtcgg
      661 cgccacggct cgagcggtat tcaatgacgc cagggcacta ctggaaaagg tcgtcagcga
      721 aaagtggttg cgcaccaacg ccgtcttcgg cgtgttcccg gccaacagcg tcggtgacga
      781 catcgaaatc tatgccgacg aaacccgttc ccaaccgtcg atggtctggc acaacctgcg
      841 ccagcagcag gaacggcccg cgggcaaacc gcactattgc ctcagcgatt tcgtcgccga
      901 ccgcggcacc ccggactgga tcggcgcgtt tgccgtcggt gccggcttcg gcatcgagga
      961 gaaactcgcc gaattcaacg ctgcgcacga tgattaccat gcgatcatgc tgaaagccat
     1021 cgccgaccgc tttgccgaag cctgcgccga gtggttgcac gcgcaggtca gacgcgacta
     1081 ctggggctac gccagcgacg aagacctcga tcgcgcagcg ctgatccgcg aggactatcg
     1141 cggcatccgc ccggcccccg gctatccggc ctgcccggac cacaccgcca agggtgcgct
     1201 gttcgagttg ctcgacgccc cccgcaacac cggcatggcg ctcaccgagt ccttcgccat
     1261 gtcgccggct gcggcggttt ccggcttcta tctggcacat ccgcaagcgc gttatttcgc
     1321 ggtcagcaag atcgccagag atcagctcga agactgggcg cagcgtaccg gcttcagcct
     1381 ctccgaagcc gagcactggc tggcgccgct gctctgagca aggccgggag cctgggcgcg
     1441 gccgtgcccg ccaagccact gccggacttc gtcgagcacg ccgtcagcct cgaaaggcgc
     1501 acgacgctgg ccctgcccgg gcaagccgcg tgctacgccg aaatccgggc ggccgcccaa
     1561 ctggcgcagc ttgccggccc cagcgggcgg cgtcagttcg ttctcggcgg cggcagcaac
     1621 ctcgttctcg gcggcgactt tgacggcctg atattgcaca tggcgattgc cggccggcaa
     1681 ctgctcggcg aggacgccga ggcatggtat gtgtacgcgg gtgccggcga gaactggcat
     1741 caactggtgc tgtggacgct gcagcagggc tggccgggac tggaaaacct ggcgctcatc
     1801 ccgggcacgg tcggcgccgc accgatccag aacatcggcg cctacgggct cgaagtcgcc
     1861 gaccgcgtcc tcagcctcga cgctgtggac atgctcaccg gcaaaacgat acggctggat
     1921 cgtgccgact gccgctttgc ctatcgcgac agcgtcttca agcaggaggg ctggcacctc
     1981 gacgcgcgtc gcgtggtgat cggggtgacc ttccggctgc cgaaagcctg gcggccactg
     2041 accggttatg gcgatctcgc ggccgaactc gaccgccg
//
LOCUS       DAOSIT010000436         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46401_length_2077_cov_10.845697, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000436 DAOSIT010000000
VERSION     DAOSIT010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46401_length_2077_cov_10.845697"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLL14_09660"
     CDS             complement(<1..1115)
                     /locus_tag="PLL14_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG04237.1"
                     /translation="MTAKPPGSAPPRASSSPFADCLAVDQRRLRSLARDLRHLFGGKR
                     EALSAEHDRLLGQSRAAVAARRARLPTPEFADDLPVNLRREEIAALIDKNPVVIVCGE
                     TGSGKTTQIPKICLEIGRGTTGLIGHTQPRRIAARATAARLAQELRGELGRLVGYKIR
                     FSDRTSPDAFIKLMTDGILLAETQGDPWLEQYDTLIIDEAHERSLNIDFLLGFLKQLL
                     PKRRDLKLIITSATIDAERFSQHFDGAPVIEVSGRLYPVEVRYRPLQTEKEETAPDDE
                     RDLYDAIADACDELHRVGPGDVLVFLPGEREIREAAEALRKHHPPHTEILPLFARLST
                     EEQGRIFKPHVGSRIVLATNVAETSLTVPGIRYVVDSG"
     gene            complement(1164..1457)
                     /locus_tag="PLL14_09665"
     CDS             complement(1164..1457)
                     /locus_tag="PLL14_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HOG04238.1"
                     /translation="MTTTPHSSFIIVGLTIAGKKFRPSDWAERLCGVLSAFGAEKKMR
                     YSPYVGPRNYKGEKAVYVDGRLYEVEPMAYRFVLHFAQDNDLQLIQDVAATSS"
     gene            complement(1558..1632)
                     /locus_tag="PLL14_09670"
     tRNA            complement(1558..1632)
                     /locus_tag="PLL14_09670"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1598..1600),aa:Val,seq:cac)
     gene            complement(1968..2075)
                     /locus_tag="PLL14_09675"
     CDS             complement(1968..2075)
                     /locus_tag="PLL14_09675"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOG04239.1"
                     /translation="MVKRILRKYRYPPDQQEAAIELVLQQAEVLGEEWL"
BASE COUNT          424 a          652 c          630 g          371 t
ORIGIN      
        1 cccgaatcga cgacgtagcg gatgccgggc acggtcagcg aggtttcggc gacgttggtc
       61 gccagaacga tccggctgcc gacatgcggt ttgaaaatcc gcccctgttc ctcggtcgag
      121 agccgagcaa acaggggcag gatctcggta tgcggtgggt ggtgcttgcg cagcgcctcg
      181 gcagcctcgc gaatctcgcg ttcacccggc agaaagacca ggacatcacc cggcccgaca
      241 cgatgcaact cgtcgcaggc gtcggcgatc gcgtcataaa ggtcgcgttc gtcgtccggg
      301 gcggtttcct ccttttcggt ctgcagcgga cgataacgca cttcaaccgg atacagccgg
      361 cccgacacct cgatcaccgg cgcgccgtca aagtgctgcg aaaagcgttc ggcgtcgatc
      421 gtcgccgagg tgatgatcag tttcaggtca cgccgctttg gcagcaactg tttgaggaag
      481 ccgagcagga agtcgatgtt caggctgcgc tcgtgcgcct cgtcgatgat cagcgtgtcg
      541 tactgctcca gccaggggtc gccctgcgtt tcggcgagca gaatgccgtc ggtcatcagc
      601 ttgatgaagg cgtctggcga agtacgatcg ctgaagcgaa tcttgtatcc gaccagacgt
      661 cccaactcgc cccgcagctc ctgcgcgagg cgtgcagcgg tggcacgtgc ggcgatgcgt
      721 cgcggctgcg tgtggccgat cagacccgtc gtgccgcgcc cgatttcgag acaaatcttc
      781 ggaatctgcg tggtttttcc cgaacccgtt tcaccgcaga cgatcaccac cgggttcttg
      841 tcgatcagcg ccgcaatttc ctcgcggcgc aagttgaccg gcagatcgtc ggcaaactcc
      901 ggcgtcggca agcgcgcgcg gcgcgcggcg acggcggcac gcgactgccc caggaggcgg
      961 tcgtgctcgg ccgacagcgc ctcacgtttg ccgccaaaaa ggtgcctgag atcgcgcgcc
     1021 agggagcgca ggcggcgctg gtcgacggcc agacaatcgg cgaaagggct gctggaggca
     1081 cgcgggggag ccgatccggg aggcttggcg gtcatcggtc agaaactacg ggcgcgcgcc
     1141 agcgcgccat tagccacaga cacctacgag ctggtcgcag cgacgtcctg aatcagttgc
     1201 agatcgttgt cctgagcaaa gtgaaggaca aaacgataag ccatcggctc aacttcgtac
     1261 aaccgaccgt ccacgtaaac cgccttctca cctttgtagt tccgcggacc aacataaggg
     1321 gaatagcgca ttttcttctc ggcgccaaaa gccgacagaa caccacagag tctctccgcc
     1381 cagtcgctcg gccgaaactt cttgcccgcg atggttagac caacgatgat gaaagaggag
     1441 tgcggcgtag tggtcatatc tcggtggtgg ggtagcgagg ttaaccgcga attactctct
     1501 tggattgtcg ttgaacgcct gccaggacgg tcgacagccc catatcaaca gctgtgatgg
     1561 tgggcgatag tgggttcgaa ccactgactt ccaccgtgtg aaggtggcac tctaccactg
     1621 agttaatcgc ccaagttcct gtttttttga tgcccggata gcgaaggcag agttggcaac
     1681 cagccagacg cagcaacggg cagttctgcc cactccatgc tgtttaggtc cagtgccacc
     1741 atcaaactga aagcgctgca ccgcatctcc accgcaagcc acaggagtag agattacatc
     1801 acaagtcgct actccggtca atcgagcacg cccacacgcc gacatccgga taccgacggc
     1861 ggcgcaagaa caggccaggc cagcggaaac acggcgaaag cgccgcgccc tgatcgagga
     1921 gcttgagagt agcccgccga gccgatcccg caccagaacg ccagcactca cagccactct
     1981 tcacccaaca cctcggcctg ctgcagcacc agctcgatgg ctgcctcctg ctgatccggc
     2041 gggtacctgt acttgcgcag gatgcgcttg accatca
//
LOCUS       DAOSIT010000437         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46403_length_2077_cov_8.678042, whole genome shotgun sequence.
ACCESSION   DAOSIT010000437 DAOSIT010000000
VERSION     DAOSIT010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46403_length_2077_cov_8.678042"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2077)
                     /locus_tag="PLL14_09680"
     CDS             complement(<1..>2077)
                     /locus_tag="PLL14_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOG04240.1"
                     /translation="ILDQATAARSMAELRIEIETLKGLEGQALAVRRSGTDTKWRELA
                     SLLAEIFTPAAIAHRVAEPPAPYGAGPIPPPTPSPHQKLVIFTEHRDTLNYLEQRITT
                     LLGRREAVVIIHGGIGREERLKVQESFKHDPAVQVLLATDAAGEGINLQRAHLMVNYD
                     LPWNPNRLEQRFGRIHRIGQTEVCHLWNLVADDTREGDVYRKLLEKLEQARQALGGQV
                     FDVLGKLEFEGKSLRELLIEAIRHGEKPEVKAYLTTVLDIALDHDHLQNLLEERVLAH
                     DAMDASRVQRIREDMERADARRLQPHYIESFFHEAFRQLGGAARQREPRRYEVTHVPA
                     PVRHRDRLIGIGEPVLPRYERIAFDKALVAPQDQPLAAFICPGHPLLDAVIDLTLERN
                     RDLLKRGTVLVDERDPGTLPRVLFYLEHAIQDAGLTHAGERRVVSKRMLYVELAADGT
                     TRHVHYAPYLDYRPLKTDEPGVAALLDRPEAQWIAHGLEQRAQGHAVAHVVPEHLAEV
                     RGRKFALIARTEAAVKERLTKEITYWDHRAEELKLQEQAGKPNARLNSNEARKRADLL
                     QGRLQKRLEDLKLEAQISPLPPVVLGGLLVVPLGLIQAMTGQATAAFTAPVDTQLSAA
                     RARAIVMAIERGLGFEPTDREVEKLGYDIESKIPGTGKLRFIEVKGRVTGAPTITVTR
                     NEILYSLNKP"
BASE COUNT          309 a          640 c          731 g          397 t
ORIGIN      
        1 ccggcttgtt gagcgagtag agaatctcgt tgcgcgtgac ggtgatcgtc ggcgcgccgg
       61 tcacgcggcc cttcacctcg atgaagcgca gcttgccggt gccgggaatt ttgctttcga
      121 tgtcgtagcc gagcttctcg acttcgcggt cggtcggctc gaaaccgagc cctcgctcga
      181 tcgccatgac gatggcgcgc gcacgggcag cgctgagttg ggtgtcgacc ggagcggtga
      241 aagcggcggt cgcctgaccg gtcatcgcct ggatcaagcc cagaggaacg accagcaggc
      301 caccgagcac gacgggcggc agcggtgaaa tctgcgcttc gagtttcagg tcttcgagcc
      361 gtttctgcag ccgtccctgc agcaggtcgg cgcgcttgcg ggcctcattc gagttgagcc
      421 tggcgttcgg cttgccggcc tgttcctgca gcttgagttc ttccgcgcgg tggtcccagt
      481 acgtgatctc cttggtcagc cgttccttga ccgccgcctc ggtcctggcg atcagggcga
      541 acttgcggcc gcgcacttcg gccaggtgtt ccggcacgac gtgggcgacg gcatggccct
      601 gcgcccgttg ctccagtccg tgggcgatcc actgcgcttc gggccgatcg aggagcgcag
      661 cgacgcccgg ctcatccgtt ttcagcgggc ggtagtcgag atagggcgcg taatggacgt
      721 ggcgcgtcgt tccatccgcc gccagttcga cgtagagcat gcgcttcgaa acgacgcggc
      781 gctcgccggc gtgggtgagg ccggcatcct gtatggcgtg ctccagatag aagagcacgc
      841 gcggcaaggt gccgggatcg cgctcgtcga ccagcacggt gccgcgcttg agaaggtcac
      901 ggttgcgctc cagcgtgagg tcgatgacgg cgtcgagcag cgggtgaccg gggcagatga
      961 aagccgccag cggctggtcc tgcggggcga ccagcgcctt gtcgaaagcg atgcgctcat
     1021 agcgcggcag caccggttcg ccgatgccga tcaggcgatc gcggtggcgc accggggcgg
     1081 gaacgtgggt gacttcgtaa cggcgaggtt cgcgctgcct ggccgctcca ccgagctgcc
     1141 tgaaggcttc atgaaagaag gactcgatat agtgcggttg caggcggcgg gcatcggcgc
     1201 gctccatgtc ctcgcggatg cgctgcaccc ggctggcgtc catcgcgtcg tgcgccagca
     1261 cgcgctcttc gagcaggttc tgcaggtgat cgtggtcaag cgcgatgtcg agcaccgtcg
     1321 tcaggtaggc tttgacttcc ggcttttcgc catggcggat cgcttcgatc aggagttccc
     1381 tgagcgactt gccctcgaat tccagtttgc cgagaacgtc gaaaacctgg ccgccaagcg
     1441 cctggcgcgc ctgttcgagc ttttccagca gcttgcgata gacatcacct tcgcgggtat
     1501 cgtccgccac cagattccac aggtggcaga cctcggtctg gccgatgcgg tggatgcggc
     1561 cgaagcgctg ttcgagccgg ttcgggttcc acggcaggtc gtagttgacc atcagatggg
     1621 cacgctgcag gttgatgccc tcgccggcgg cgtcggtcgc cagcagcacc tgcaccgcgg
     1681 ggtcgtgctt gaacgattcc tgcaccttca gacgctcttc gcggccgatg ccgccgtgga
     1741 tgatgaccac ggcttcccgg cggccgagca gcgtggtgat gcgctgttcg agatagttga
     1801 gcgtgtcgcg gtgctcggtg aagatgacga gcttctgatg cggcgaaggc gttggcggtg
     1861 ggatcggacc ggcgccatag ggagccggcg gttcggcaac gcggtgggct atggccgccg
     1921 gcgtgaagat ttcagcaagc agactggcga gttcgcgcca cttggtgtcg gtgccactgc
     1981 ggcgcacggc cagcgcctgg ccttcaagcc ccttcagcgt ttcgatctcg atgcgcagtt
     2041 cggccatcga gcgggccgcg gtcgcctgat cgaggat
//
LOCUS       DAOSIT010000438         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46754_length_2065_cov_8.852736, whole genome shotgun sequence.
ACCESSION   DAOSIT010000438 DAOSIT010000000
VERSION     DAOSIT010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46754_length_2065_cov_8.852736"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1192
                     /locus_tag="PLL14_09685"
     CDS             80..1192
                     /locus_tag="PLL14_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HOG04241.1"
                     /translation="MIKARAAVAWAARQDLEITEVDVEAPRQGEVLVRIVATGVCHTD
                     AFTLSGADPEGIFPSILGHEGGGIVEAVGPGVTSVGIGDHVIPLYTPECGQCKFCRSG
                     KTNLCQAIRTTQGRGLMPDGSSRFSRNGTPIYHYMGTSTFSEYTVLPEISVARIAKDA
                     PLEKVCLLGCGVTTGIGAVINTAKVEPGATVAVFGLGGIGLSVVIGAVMARASRILAV
                     DTNPGKFAIARQLGATDCINPADYDRPIQEVIVDLTDGGVDYSFECIGNVKVMRSALE
                     CCHKGWGESVIIGVAGAGEEIATRPFQLVTGRVWRGSAFGGVRGRSELAGYVERAQSG
                     EIPLDTFITHTMGLEDINHAFELMHEGKSIRSVIRF"
     gene            1196..2056
                     /gene="fghA"
                     /locus_tag="PLL14_09690"
     CDS             1196..2056
                     /gene="fghA"
                     /locus_tag="PLL14_09690"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HOG04242.1"
                     /translation="MTEPAFAALEEVAANRCFGGWHRRFRHRSATLRCEMVFAVFLPP
                     HAERATVPALYWLSGLTCSDENFMQKAGAMRVAAELGIALVAPDTSPRGTHVPGDPDG
                     AWDFGHGAGFYLNATQEPWAAHYRMHDYVVAELPELIEANLPIDARRGISGHSMGGHG
                     ALLCALRNPGRYRSVSAFAPICHALASPWGQKAFLRYLGEDRAAWQDWDVCELLVGAR
                     ERLPLLVDQGDADGFLVEQLQPELLQQACAAAGHSLDLRLQPGYDHSYYFIASFIEDH
                     LRHHAGALTP"
BASE COUNT          355 a          606 c          681 g          423 t
ORIGIN      
        1 gggatcgaag tgttccgctg gccgtacatg aaaccggggg atctgcactg atcttcgcat
       61 cgttcaagga gagtcgttga tgatcaaagc acgcgctgca gtggcctggg ctgccaggca
      121 agacctcgaa atcaccgaag tcgacgtcga agcacctcga cagggtgagg tgttggtacg
      181 tatcgtcgcc accggggtct gccatacgga tgcgttcacg ctttcgggcg ccgatccgga
      241 agggatcttt cccagtatcc tcgggcatga aggcggtggc atcgtggaag ccgtcggtcc
      301 cggagtgacc tcggtaggaa ttggcgatca cgtcataccc ttgtacacgc cggagtgtgg
      361 gcagtgcaag ttctgccgct cgggcaagac gaatctgtgt caggccatcc gaacgactca
      421 gggcaggggt ctgatgcccg atggcagtag tcgcttttcc cgtaacggca caccgatcta
      481 ccattacatg gggacttcca ccttctccga gtacacggtg ttgcccgaaa tttcggtggc
      541 cagaattgcc aaggatgcac cgctggaaaa ggtctgtctg ctcggctgcg gggtgacgac
      601 gggtatcggc gcggtgatca ataccgccaa agtcgagccg ggtgccaccg tggccgtttt
      661 cgggctgggc ggcatcggtc tttcggtagt gatcggggcg gtgatggcca gggcttcgcg
      721 gatccttgcc gtcgatacca atcccggcaa atttgccatc gccaggcaac tcggcgccac
      781 cgactgcatc aatcccgccg attacgaccg gcctatccag gaagtgatcg tcgacctgac
      841 cgacggtggg gtcgattact cgttcgaatg tatcggcaac gtcaaggtga tgcgctcggc
      901 gctcgagtgc tgtcacaagg gttggggtga atcggtgatc attggtgtcg ctggtgccgg
      961 tgaagagatc gcgacccgac cgttccagtt ggtgaccggc agggtgtggc gcggttcggc
     1021 gttcggtggc gtgcgtggcc gctccgaact cgcgggttat gtcgaacgcg cgcaaagcgg
     1081 cgagattccg ctcgacacct tcattaccca tacgatgggc ctggaggata tcaatcatgc
     1141 tttcgagctg atgcatgaag gcaagagcat tcgctccgtg atccggttct gagcggtgac
     1201 ggagcccgct tttgcagcgc ttgaggaggt cgcagccaat cgttgttttg gcggctggca
     1261 tcggcgcttc cggcatcgct cggcgaccct gcgctgcgag atggtgtttg ccgtcttcct
     1321 gccgccgcac gccgagcggg cgacggtgcc cgccctgtac tggctgtcgg gcctgacctg
     1381 cagcgatgag aacttcatgc agaaagccgg agcgatgcgc gtcgcggccg agttggggat
     1441 cgccctggtg gctccggata ccagcccgcg cggtacacac gtgccgggcg acccggacgg
     1501 tgcctgggat ttcggtcacg gcgccgggtt ctatctgaac gctactcagg agccatgggc
     1561 agcacattac cggatgcacg actatgtggt tgctgaactg ccggagttga tcgaggcgaa
     1621 cctgccgata gacgcgcgcc ggggtatcag tggccattcc atgggcggac atggcgcgct
     1681 gctttgcgct ctgcgcaatc ctggccgcta ccgctcggtg tccgccttcg cgccgatctg
     1741 tcatgccttg gccagcccct gggggcagaa ggccttcctg cgctatctcg gcgaagaccg
     1801 tgcggcatgg caagactggg acgtctgcga actgctcgtc ggtgcccgcg aacgtctgcc
     1861 cctgctggtc gatcaaggtg acgcggacgg gtttctcgtc gagcaactgc agcccgaatt
     1921 gttgcagcag gcctgcgccg cagcagggca ttcgctggat ctgcgtctgc agccgggtta
     1981 cgaccatagc tactacttca tcgccagttt catcgaagat cacctgcgcc atcacgccgg
     2041 ggcgctgacg ccatgagtcg ctcgc
//
LOCUS       DAOSIT010000439         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46780_length_2064_cov_11.815331, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000439 DAOSIT010000000
VERSION     DAOSIT010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46780_length_2064_cov_11.815331"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLL14_09695"
     CDS             <1..990
                     /locus_tag="PLL14_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOG04243.1"
                     /translation="MARAAVGLPRNTGWEHPICQRIGRLTAAARIALDTRGAPLDAHL
                     ADLDTLIRERDQSIRRAAENSIQMVVEHENRQYAEQLAATWLRGRLARTRSPEVASFL
                     EKYWLRVMVAAVLEDGAAGKRWQESSTTGDELIWSVQPKQSAEERKRLASLASSLVRR
                     IGAGLDAIGVPAAERSPFLNALFDLQTAALRSQAPTPPSPGTAADSPRAIVGGASPAA
                     PRVLERDGWRVHYLGMPSDTRSPHTLAVANWQVGDWLRFRMANTEPLCGLGCWQNPSS
                     GTILFFNPDWPYAVAMSAATVDQQIRSGRAQLVSRVAIFDAAAERALGLLEKR"
     gene            1181..>2064
                     /locus_tag="PLL14_09700"
     CDS             1181..>2064
                     /locus_tag="PLL14_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG04244.1"
                     /translation="MRFDELGLAPELLRAIADKGYTEPTPIQAQAIPLVLAGKDLMGG
                     AQTGTGKTAAFTLPLLQRILAFASPSPSPARHPVRVLMLAPTRELAIQVHESVKIYSR
                     HVPIRSACVFGGVDIKPQIAEIRCGVEVLVATPGRLLDLVEQKCLNFGSVQALVLDEA
                     DRMLDMGFVPDVTRIINMLPRQRQSLLFSATFSEEIKKLADGMLQSPVLIEVAKRNTV
                     SETITHRVHPVAAEAKRALLVKLLKSADFNQVLVFTRTKIETNKLARELQRAGIAADS
                     IHGDKSQQERLKALEAFK"
BASE COUNT          396 a          695 c          647 g          326 t
ORIGIN      
        1 atggcgcggg cggcggtcgg cctgccgcgc aacaccggct gggaacatcc gatctgccag
       61 cgcatcggcc gactcaccgc agccgccagg atcgcgctgg acaccagggg tgcgccgctc
      121 gatgcacacc tcgccgacct ggacacgctg atccgggaac gcgaccagtc gatccgacgg
      181 gcggcggaga actccatcca gatggtcgtc gagcacgaga accggcagta tgccgaacaa
      241 cttgcggcga cctggctgcg cggcaggctg gccagaacac gttcgcccga ggtcgcgtcg
      301 tttctcgaaa agtactggtt gcgagtgatg gtcgccgctg ttctggagga cggcgccgcg
      361 ggcaaacgct ggcaagagag cagcactacc ggcgatgaac tgatctggag cgtacaacca
      421 aaacaaagcg ccgaggaacg caagcgcctc gccagcctgg cctcgtcgct ggtccggaga
      481 attggcgccg ggcttgacgc catcggcgtt cctgccgccg agagaagccc gtttctgaat
      541 gccttgttcg acctgcagac ggccgcgctg cgcagccagg cgccaacccc gccgtcaccg
      601 ggaacggctg cggacagccc tcgcgccatc gtcggcggcg cgtcgccggc agccccgcgc
      661 gtcctcgagc gcgacggctg gcgggtacac tatctgggaa tgccgtccga tacccgctca
      721 ccgcacacgc tggcggtggc caactggcag gtcggcgact ggttgcgttt ccggatggcg
      781 aacaccgagc cgctgtgcgg cctcggttgc tggcaaaacc cgtcctcggg gacgatcctg
      841 ttcttcaacc cggactggcc ctacgccgtc gccatgtcgg cagccaccgt cgaccagcaa
      901 atccgctccg gccgcgcgca gctcgtttcg cgagtcgcca ttttcgacgc ggccgcagaa
      961 cgcgcactcg gcctgctcga aaaacgctga cggcacgccg gaaattcgcg caaaacccgg
     1021 cccgtgcttg cctgcagcga tcgctgaagt ggctcggcgc caccgcagcg acgctccgac
     1081 ccgcgtctgc cgccgcaaag caggccggca ggggcgcgcg atcgatgtat aatcacgctc
     1141 catatgagat tgatttttga ccgatttgaa ggctttgccc atgcgttttg atgaactcgg
     1201 cctggcacct gaactgctga gagccattgc cgacaagggc tacacagagc caacgccgat
     1261 ccaggcacag gcgataccgc tcgttctcgc cggcaaggat ctgatgggcg gcgcccagac
     1321 gggcaccggc aagaccgccg cgttcacgct gccgctgctg cagcgcatcc tggccttcgc
     1381 cagccccagc ccttcaccag cccgccaccc ggtacgcgta ctgatgctgg cgccgacacg
     1441 cgaactggcg atccaggtcc acgaatcggt gaaaatctat agccgacacg taccgatcag
     1501 gagtgcctgc gtcttcggtg gggtagacat caaaccgcag atcgccgaga tccgctgcgg
     1561 tgtcgaggtc ctggtagcca cccccggtcg gctgctcgac cttgtcgaac aaaagtgcct
     1621 caacttcggc agcgtgcagg cgctcgtgct cgacgaggcg gatcgcatgc tggacatggg
     1681 cttcgtcccg gacgtcacgc gcatcatcaa catgctgcct cggcagcggc agagcctgct
     1741 gttctcggcg accttttcgg aagagatcaa gaaactggcc gacggcatgc tgcagtcgcc
     1801 ggtactgatc gaggtcgcca agcgcaatac ggtgtcggaa acgatcaccc accgggtcca
     1861 tccggtggcc gccgaggcca agcgtgcgct gctggtgaag ctgctgaaat cggccgactt
     1921 caaccaggtg ctggtattta cgcgcaccaa gatcgaaacc aacaaactgg ctcgcgagtt
     1981 gcaaagggca ggaattgccg ccgactcgat ccacggtgac aagagccagc aggaacgcct
     2041 caaggcactc gaagcgttca agga
//
LOCUS       DAOSIT010000440         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46781_length_2064_cov_7.303136, whole genome shotgun sequence.
ACCESSION   DAOSIT010000440 DAOSIT010000000
VERSION     DAOSIT010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46781_length_2064_cov_7.303136"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PLL14_09705"
     CDS             <1..1073
                     /locus_tag="PLL14_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOG04245.1"
                     /translation="PAGSGLVAFAAATGVMPLDMPESVLVRFKGSLAEAGKRGVTLRD
                     LVNAIPYYAIQAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELSDAAAERSAAA
                     CAVALNKEPIIEYMRSNITLMKWMIANGYSDARTLGRRIKAMQAWIAKPELLRGDADA
                     QYAAVIEIDLAEVTEPLVACPNDPDDVKLLSAVSGTKIDEVFIGSCMTNIGHFRAAGK
                     VLEGKTDIPTRLWIAPPTKMDAMILNEEGYYAILGKSGARMEMPGCSLCMGNQAQIRK
                     GSTAMSTSTRNFPNRLGIDTNVYLGSAELAAVCALMGRIPTPAEYLEQVQAVNAKAAD
                     VYRYMNFDQIREFSDVADTVTV"
     gene            1165..1419
                     /locus_tag="PLL14_09710"
                     /pseudo
     CDS             1165..1419
                     /locus_tag="PLL14_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
     gene            1543..1733
                     /locus_tag="PLL14_09715"
                     /pseudo
     CDS             1543..1733
                     /locus_tag="PLL14_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            1741..1953
                     /locus_tag="PLL14_09720"
     CDS             1741..1953
                     /locus_tag="PLL14_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG04246.1"
                     /translation="MRYAIVIEKAESNYSAYVPDLPGCIATGANIQQVEAEIREAIEF
                     HINGLREDGSQIPLPSSQVEYIEIAA"
     gene            complement(1877..>2064)
                     /locus_tag="PLL14_09725"
     CDS             complement(1877..>2064)
                     /locus_tag="PLL14_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04247.1"
                     /translation="LGQSQALDELAQRRDDALADFDAAGNAENQGFVALRVMPRFRCT
                     QPDWTARGSGFRLLASR"
BASE COUNT          400 a          664 c          637 g          363 t
ORIGIN      
        1 tcccggccgg ttccggtctg gtcgcctttg ccgccgccac cggcgtcatg ccgctcgaca
       61 tgccggaatc ggtgctggtg cgcttcaagg gctcgctcgc cgaagccggc aagcgcggcg
      121 tcacgctgcg cgacctggtc aacgcaattc cctattacgc catccaggcc ggcctgctga
      181 ccgtcgagaa gaagggcaag aagaacatct tctcgggccg catcctcgaa atcgaagggc
      241 tgccggatct caaggtcgaa caggccttcg aactctccga cgccgctgcc gagcgttcgg
      301 ccgccgcctg tgcggtggcg ctgaacaagg aaccgatcat cgagtacatg cgctcgaaca
      361 tcacgctgat gaagtggatg atcgccaacg gctactccga cgcccgtacc ctcggccgcc
      421 gcatcaaggc catgcaggcc tggatcgcca aaccggaact gctgcggggt gacgccgacg
      481 cgcagtacgc cgcggtgatc gagatcgacc tcgccgaggt caccgagccg ctggtcgcct
      541 gcccgaacga cccggacgac gtcaaactgc tctccgccgt ctccggcacg aagatcgacg
      601 aagtcttcat cggctcgtgc atgaccaaca tcggccactt ccgcgccgcc ggcaaggtcc
      661 tcgaaggcaa gaccgacatc ccgacgcgcc tgtggatcgc gccgccgacc aagatggacg
      721 cgatgatcct caacgaggaa ggctactacg cgatcctcgg caagtccggc gcgcgcatgg
      781 aaatgcccgg ctgctcgctg tgcatgggca accaggcgca gatccgcaag ggcagcacgg
      841 cgatgtcgac ctcgacgcgc aacttcccga accgtctcgg catcgacacc aacgtatatc
      901 tcggctcggc cgaactcgcc gccgtctgcg cgctgatggg caggatcccg acgccggccg
      961 agtatctgga gcaggtgcag gcggtcaatg ccaaggctgc agatgtctat cgctacatga
     1021 acttcgatca gatcagggag ttcagtgacg tggcggatac cgtgacggtg taggcgtcaa
     1081 ctcagcggct aactgaaacg cccccggaag gagactcccg ggggcgtttt tcactgcgac
     1141 atgatcgatc gcgaagtgct ggatctggtc accggacccg tgacgcgcaa cagggcagag
     1201 gccttgcggc atacctgtga cgccaccccg gcactaaaac tggtcgtcgc cgtcggcgac
     1261 tgcgacggcg gcatcttcgg cgcgagctgc gcggtttgcg gtcgcgtggc cgatgtcgcg
     1321 gtgcccggtt gcccgccgcc gctcgagaag cagctgtgcg ggattctgac ggcggtgcgc
     1381 tgctgtcggg cgccgtcggc ccaaggggtt accgcctgat ccagccgacc tgcgggcggg
     1441 gctgccctgt cgttcttcgc gctgcgcccc tgagcttgga cctcatgccg ctcgctatcg
     1501 gcgcgaattg tttgcaagtg gacaagcgga tattcttcgc ggatgaaaat cagtgaaatt
     1561 ctgcgtatgc ttcaagctga tggctggttt ctggtagcga cccgaggaag ccccatcgac
     1621 agtacaaaca tcctgtgaaa cctggccggg tcaccgtgcc tggtaagcca agcgacgatg
     1681 ttgctcccgg tacattgaac agcatcatga agcagtccgg ttcgaagcag tgaggtcacc
     1741 atgcgttacg ccatcgtaat tgaaaaggct gaatcaaatt actctgccta cgtcccggac
     1801 ttaccggggt gcatcgctac tggtgcaaac attcagcagg tcgaggcgga aattcgtgaa
     1861 gccatcgagt tccatatcaa cggcttgcga gaagacggaa gccagatccc cttgccgtcc
     1921 agtcaggttg agtacatcga aatcgcggca taacccggag ggcgacgaag ccctgattct
     1981 cggcattgcc cgccgcgtcg aaatcggcca gcgcatcgtc gcgccgctgc gccagctcgt
     2041 cgagggcctg cgactggcct agca
//
LOCUS       DAOSIT010000441         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_46978_length_2058_cov_6.482277, whole genome shotgun sequence.
ACCESSION   DAOSIT010000441 DAOSIT010000000
VERSION     DAOSIT010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46978_length_2058_cov_6.482277"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="PLL14_09730"
     CDS             complement(<1..1838)
                     /locus_tag="PLL14_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG04248.1"
                     /translation="MDHGDCYHCGQAVATGVDLSVNIDGLARGMCCGGCQAVAQAIVD
                     NGLSDYYRNRDALPNAPREALPAIVEGLQLYDHADFQKGFVRVRGEGPDSSEREASLI
                     LEGITCSACIWLNEQHLSRLPGVTAVDINYATRRARVCWDERRVRLSQILSAIAAIGY
                     RAFPYDTAKSEELARKERRAALWRVFVAGFGMMQVMMYAVPVYLAGDGEMTPAVEQLM
                     RWASLVLTLPVVFYSAAPFFRNAWRDLRLARVGMDVPVALGVGAAFAASVWATLTAGG
                     EVYFDSVTMFVFFLLSGRFLEMTARQRAVSVTEALARLMPVVAARMPAYPDQRNLEQV
                     MAADLRAGDVVLVKPGETIPADGRVIEGESSANEALLTGESAPVRKRPGVPVTGGAVN
                     IESPLLVEVAQVGEGTRLSAIVRLMERAAMEKPRIVELADRIASRFIICLLFLAVGVA
                     VAWSFIDPRQALWITVSVLVVSCPCALSLATPVALTVAGGAMARAGLLVTRSHAIETL
                     ARATHFVFDKTGTLTTGEMHVLEVLPLGTLSRDQSLALAAALEQASEHLIGKALRAAM
                     AGRVMPAVVGLASEPGYGVRAQYGGRQVRVGRPDYVGKLHGGPLPD"
     gene            complement(1879..>2058)
                     /locus_tag="PLL14_09735"
     CDS             complement(1879..>2058)
                     /locus_tag="PLL14_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04249.1"
                     /translation="LAERRIKLDEALKLAQNATLLAPKDPNVFDTLAAVYRARGDANE
                     ERKAQAQARQLGKRG"
BASE COUNT          385 a          765 c          593 g          315 t
ORIGIN      
        1 ctgtccggca agggcccgcc atgcagtttg cctacatagt ccggccgccc gacgcgcacc
       61 tggcggccgc cgtactgcgc acgcacgcca tacccgggct cgcttgccag gcccacgaca
      121 gccggcatga ctcgcccagc catcgccgcc cgcagcgctt tgccgatcag atgctcggac
      181 gcctgctcta gagcagccgc cagcgccaga ctctggtccc gactgagcgt accgagcggc
      241 aaaacttcga gcacgtgcat ctcgccggtg gtcagcgtgc ccgtcttgtc gaacacgaaa
      301 tgggtagccc gcgcgagggt ttcgatggca tgactgcgcg tcaccagcaa ccctgcgcgc
      361 gccattgctc cgccggcaac ggtcagcgcc accggggtag ccagcgacag cgcacaggga
      421 caactgacga ccagcaccga aaccgtgatc cacagcgcct gccgggggtc gatgaacgac
      481 caggcgacgg ccacgccgac cgccaggaac aacagacaga tgatgaaacg actggcgatg
      541 cgatcggcca gttcgacgat ccggggtttc tccatcgccg cacgttccat cagccggacg
      601 atcgccgaca ggcgcgtgcc ttcgccgacc tgcgccacct cgaccagcag ggggctttcg
      661 atattcactg caccaccggt cacgggcacg ccggggcgct tgcgaaccgg cgcactctcc
      721 ccggtgagca gtgcctcgtt ggcactgctt tccccctcga tgacccgccc atccgccgga
      781 atggtttccc cgggtttgac caggacgaca tcgcccgcgc gcaggtcggc ggccatcacc
      841 tgctccaggt tgcgctgatc cggatacgcg ggcatgcgcg ccgcaaccac cggcatcagc
      901 ctggccagtg cctcggtgac gctgaccgca cgctgacgag cggtcatttc caggaagcga
      961 ccgctcaaca agaagaagac gaacatcgtc accgagtcga aatagacttc cccgccggca
     1021 gtcagcgtcg cccagacgct ggccgcgaaa gccgcgccca cccccagcgc cacgggcaca
     1081 tccatgccca cccgagcgag tcgcagatcg cgccaggcat tgcggaaaaa tggcgccgcc
     1141 gaatagaaca ccaccggcag cgtcaacacc agactggccc agcgcatcaa ctgttcgacc
     1201 gccggcgtca tctcgccgtc gcccgccaga tacaccggta cggcgtacat catcacctgc
     1261 atcatgccga agccggcgac aaacacccgc cacagcgcag cccgccgctc cttgcgcgcc
     1321 agttcctcgc tcttcgccgt atcgtaggga aaagcgcgat agccgatcgc ggcaatcgcc
     1381 gaaagaatct gcgacagcct gacccgccgc tcgtcccagc agacacgcgc acgccgcgtc
     1441 gcgtagttga tgtccaccgc ggtcaccccg ggcaggcgcg acaggtgttg ctcgttcagc
     1501 cagatacacg ccgaacaggt gatcccttcg aggatcagcg acgcctcgcg ctcgctgctg
     1561 tcaggtccct cgccgcggac gcgcacgaaa cccttctgga aatctgcgtg atcataaagc
     1621 tgcaagccct cgacgatcgc cggcagggct tcgcgcgggg cattcggcaa ggcatcacga
     1681 ttgcggtagt agtcgctcag gccgttgtcc acaatcgcct gcgccactgc ctgacagccg
     1741 ccacaacaca tgccgcgcgc cagcccgtcg atatttaccg agagatccac tccggtcgcc
     1801 acggcctgtc cgcagtggta gcaatcgcca tggtccatca ctgccaggag gaagtcgacc
     1861 gcgctggttg acccgccctc agccgcgttt cccgagttgc ctggcctgcg cctgcgcttt
     1921 cctctcctcg ttggcgtcgc cacgcgcccg gtacaccgcc gccagagtat cgaacacatt
     1981 cgggtccttc ggcgccaaca gcgttgcatt ctgagccagc ttcagcgcct catcgagttt
     2041 gatcctgcgc tcggcaag
//
LOCUS       DAOSIT010000442         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47133_length_2053_cov_9.602102, whole genome shotgun sequence.
ACCESSION   DAOSIT010000442 DAOSIT010000000
VERSION     DAOSIT010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47133_length_2053_cov_9.602102"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="PLL14_09740"
     CDS             complement(<1..1835)
                     /locus_tag="PLL14_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOG04250.1"
                     /translation="MNEAVIHFHQTGPLLALVGNPNCGKTALFNLLTGSRQKVANYAG
                     VTVERKEGRLTTPAGRTLRVLDLPGAYSLYPRSPDERVTCDVLFGRAAGEKRPDLVVC
                     VVDATNLRRNLRLALAVKRLGLPCVVALNMTDLAEKRGLRIVPESLSAELGMPVVSTV
                     AIRHTGAEPLLALFDDQQTWRRATAGTPIPGHAAPIGDPHSNQSDHVAVRQILQNLGL
                     DEIVPHTPSDRIDRVVLHPIIGPLLLASILFLVFQLVFAWAETPMSLIKAATTEVGQA
                     IGTALPDTWIRSLIVDGVIAGAGGVLVFLPQIVILYFFILLLEESGYLPRAAFLLDRL
                     MGGVGLSGRSFIPLLSSFACAIPGIMAARTIANPRDRLVTIMIAPLMTCSARLPVYAL
                     LIGAFVPQREVYGLLELQGLVLLALYLAGIVGALAVAWGLKRFTAAGREVRPLMMELP
                     NYHWPTPLNVGLGVWQRVAIFLKRVGGIILILTILLWLLASFPAPPEGATGPAIEYSL
                     AGMLGQALAVVFEPIGFNWQISIALVPGLAAREVAVSALGTVYALAAAGEDTAQALAP
                     LIAANWSLATALSLLAWYVFAPQCLSTLATVKRETGGWTMPLLMAG"
     gene            complement(1832..>2053)
                     /locus_tag="PLL14_09745"
     CDS             complement(1832..>2053)
                     /locus_tag="PLL14_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG04251.1"
                     /translation="YVQPPTAAPEWARWLEEIGFIVGERVMLMARGLPGGDPLVVRVA
                     QSTFALRRAEAACIGVTPVNPTDTQDIET"
BASE COUNT          378 a          664 c          690 g          321 t
ORIGIN      
        1 cccgccatca gcaggggcat cgtccagcca ccggtctcgc gtttgaccgt tgccagcgtc
       61 gataggcatt gtggcgcgaa gacgtaccag gccagcagtg atagcgcggt ggccaggctc
      121 cagtttgcgg cgatcagcgg cgccagcgcc tgcgcggtgt cctcgcctgc cgccgcgagc
      181 gcgtataccg tgcccagggc gctgaccgcg acttcgcgcg ccgccagacc agggaccagc
      241 gcgatactga tctgccagtt gaagccgatc ggttcaaaca ccaccgccag cgcctgtccc
      301 agcatgcccg ccaggctgta ctcgatcgcc ggtccggtgg cgccttccgg tggtgcggga
      361 aagcttgcca gaagccacag caggatcgtg agaatgagga tgatgccgcc aacccgcttg
      421 aggaagatcg ccacccgttg ccagacgccg aggccgacgt tgagcggcgt cggccagtga
      481 taattgggca gttccatcat cagcggacgg acctcgcgtc ccgccgcggt gaagcgcttc
      541 agcccccagg cgacagccag ggcgccgacg atgccggcaa ggtacaaagc cagcaacacc
      601 agcccctgca gttccagcag cccataaacc tcacgctgcg gcacgaatgc gccgatcagc
      661 agcgcgtata ccggtagcct tgccgaacag gtcatcagcg gtgcgatcat gatggtgacc
      721 aggcgatcac gcgggttggc gatggtgcgc gcggccatga tgccggggat ggcacaggca
      781 aaactcgaca gcaaggggat gaacgagcgg ccgctgagcc ccacgccgcc catcaggcgg
      841 tcgagcagga acgctgcgcg cggcagatag ccggactctt cgagcaggag aatgaagaaa
      901 tagagaatca cgatctgcgg caggaacacc aggaccccgc cggcgccggc gatcacgccg
      961 tccacgatca ggctcctgat ccacgtgtcc ggcaaggccg taccgatcgc ctggcccact
     1021 tcggtggtag ctgccttgat caggctcatc ggtgtttcgg cccaggcgaa gaccagctgg
     1081 aacaccagga acaggattga cgccagtagc agcggtccga tgatcgggtg caggacgacg
     1141 cggtcgatgc ggtcgctcgg cgtgtgcggc acgatctcgt ccagacccag attctgcagg
     1201 atctggcgca ccgcgacatg gtcggactgg ttgctgtggg gatcaccgat gggggccgca
     1261 tggcccggga tgggcgtgcc cgccgtagcg cggcgccagg tctgctgatc gtcgaacagc
     1321 gccagcaagg gttccgcgcc cgtgtgcctg atcgcgacgg tgctcaccac cggcattccc
     1381 agttcggcgg acaggctttc cggtacgatc ctgagtccgc gtttctcggc cagatcggtc
     1441 atgttgaggg cgaccacgca gggcaggccg agacgcttca cggccagtgc cagtcgcaga
     1501 ttgcgccgca gattggtggc atcaacgacg cagaccacga ggtccgggcg cttttcgccg
     1561 gcggcacggc caaacaggac gtcacaggtc acccgctcgt caggcgaacg cggatacagg
     1621 ctgtaggctc ccggcagatc gagaacgcgc agcgtcctgc cggcgggggt cgtgagccgc
     1681 ccttccttgc gttccacggt aaccccggcg tagttggcga ccttctgccg actaccggtg
     1741 agcaggttga acagggcggt cttgccgcag ttggggttgc ccaccaaagc gagcaggggg
     1801 ccagtctggt gaaagtgaat gacggcctcg ttcatgtctc gatgtcctgg gtgtcggtcg
     1861 ggtttaccgg ggttactccg atgcaggcgg cctcggccct gcgcaaggcg aaggtagact
     1921 gggcaacgcg caccaccagc gggtcgccgc cgggtaggcc gcgggccatc agcatcaccc
     1981 gttcgccgac gatgaaaccg atttcctcca gccagcgtgc ccattccggc gcggcagttg
     2041 gcggctgaac gta
//
LOCUS       DAOSIT010000443         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47336_length_2047_cov_6.794177, whole genome shotgun sequence.
ACCESSION   DAOSIT010000443 DAOSIT010000000
VERSION     DAOSIT010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47336_length_2047_cov_6.794177"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1737
                     /locus_tag="PLL14_09750"
     CDS             238..1737
                     /locus_tag="PLL14_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOG04252.1"
                     /translation="MPLAIVHSRGLDGLSAPEVAVEVHLAGGLPSLTLVGLPDTEVKE
                     ARDRVRAALQNSGFDFPGKRITVNLAPAELPKESGRFDLPIALGILAASGQIPSAALA
                     NHEFAGELSLSGELRPIRGALAMVLAAGGVGRSFVLPTSSAREAALASKVRVLTARTL
                     LDVCAHLTGQGALDECQATDATADDGSDYPDLGEVRGQVQAKRGLEIAAAGAHSLLLA
                     GPPGTGKSMLASRLPGLLPPMTLTAALESAAVLSLAGQFRPEVFRRHPYRAPHHTASS
                     AALVGGGSIPRPGEISLAHQGVLFLDEIPEFDRRVLEALREPLDSGRIHISRAARQAE
                     FPAQFQLVAAMNPCPCGYHGDSRGRCRCTPDQVMRYRSRISGPLLDRIDLQIEVPAVP
                     AEVLQQPPDGEASATVRARVTQARERQMQRQGKPNARLTGKEIDQYCQPQADAAALLK
                     HAISRFELSARAFHRLLKVARTIADLAGSESIEAPHVAEAVQFRRFAKD"
BASE COUNT          329 a          694 c          651 g          373 t
ORIGIN      
        1 tccggctacc gctttcggga aaatggcgtc ctctggtgga gtagcctcgg ttttctggaa
       61 cgtttgagaa tcacgatctt cggttgaaag caacgggacg tatgggatga cttcaggtgg
      121 agaaagcccg tcggcgagtc cgcttcgacg ctcattcgag cacggatgtt ttgccatccg
      181 gctaccccct tttttcctcc ggattgttta gactgcggtt ttcccagagt cagcgtcatg
      241 ccgctcgcca tcgtccacag ccgtggtctc gacggactct ctgctccgga ggtcgcggtc
      301 gaggtgcatc tcgcgggcgg cctgccgagc ctcaccctgg tcgggctgcc cgatacggag
      361 gtcaaggagg cgcgcgatcg ggtacgtgcg gcactgcaga actcgggctt cgattttccc
      421 ggcaagcgca ttaccgtcaa cctcgcgcca gccgagttgc ccaaggaatc ggggcgcttc
      481 gatctgccga tcgcgctcgg cattctcgcc gcctccggtc aaatcccgag tgcggcgctg
      541 gccaaccatg aatttgccgg tgaactgtcg ctctccggtg aattgcgacc gatccgcggg
      601 gcgctggcca tggtcctcgc cgctggtggc gtgggacgca gtttcgtcct cccgaccagc
      661 agcgcccgcg aagcggcttt ggccagcaag gtgcgggtgc tgacggcgcg cacgctgctc
      721 gacgtctgcg cacacctgac cgggcaaggc gcgctggacg aatgccaggc caccgatgcg
      781 acagccgatg acggcagcga ttaccctgac ctcggcgagg tacgaggcca ggtacaggcc
      841 aagcgggggc tggaaatcgc tgccgccggc gcgcactcgc tccttctcgc cggccctccg
      901 ggtaccggca aatcgatgct cgccagccgc ctgccaggcc tgctgccgcc gatgacgctg
      961 accgctgcgc tcgaatcggc ggccgtgttg tcgctggccg ggcagtttcg tcccgaggtc
     1021 tttcgccgcc atccctaccg tgcaccgcac catacggcgt catcggcggc gctggtcggt
     1081 ggcggcagca taccccggcc cggcgagatc tctctggcgc atcagggcgt cctgtttctc
     1141 gacgaaattc ccgagttcga tcggcgggtg ctcgaagccc tgcgcgaacc gctggactcg
     1201 gggcgcatcc acatctcgcg tgcggcaagg caagcggagt ttcccgccca gttccagctc
     1261 gtcgcggcga tgaacccctg tccctgcggt tatcacggcg attcgcgcgg ccgctgccga
     1321 tgtacgccgg accaggtgat gcgttatcgc agccggatct cggggccgct gctcgaccgc
     1381 atcgacctgc agatcgaagt gcccgcggtg ccggcggagg tcctgcaaca gccacctgac
     1441 ggcgaagcgt cggctaccgt tcgggcacgt gtcacgcagg cgcgggaaag gcagatgcag
     1501 cgacagggaa aacccaatgc gcgcctgacc ggcaaggaga tcgaccagta ctgccagccg
     1561 caggcggacg cggcagcgct gctcaagcac gccatcagtc gcttcgaact ctcggcgcgc
     1621 gctttccacc gcctgctcaa ggtggcgcgg acgatcgccg atcttgcggg tagcgaatcc
     1681 atcgaggccc cgcatgtggc ggaagccgtg cagttccgcc gcttcgcgaa ggattgattt
     1741 cccgaacagg acaattccac cggcgacgcg cggcattacc gtggtcctgg gcgctctggc
     1801 ctcactcgga cccacctcga tcgacaccta cctgccatca ttcaacgaca tcagtgcttc
     1861 cctgcttgcc acaccgcgcg aagtccaaca gaccctgacc gctacctgct gccctaagtg
     1921 ctgatgacac tgtggcatgg tgtgctcttg gctgcactcg gtcgcactcg cgccagcgat
     1981 cgcgccgatg ctcggcctga gggtcaccgc atctgccggg catgggtctc gcgggcaaac
     2041 gggatca
//
LOCUS       DAOSIT010000444         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47340_length_2047_cov_6.030622, whole genome shotgun sequence.
ACCESSION   DAOSIT010000444 DAOSIT010000000
VERSION     DAOSIT010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47340_length_2047_cov_6.030622"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLL14_09755"
     CDS             complement(<1..128)
                     /locus_tag="PLL14_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOG04253.1"
                     /translation="MNIFVGNLALETDETELTDLFKAFGQVKSVQVMRELFSGASKG"
     gene            complement(183..1565)
                     /locus_tag="PLL14_09760"
     CDS             complement(183..1565)
                     /locus_tag="PLL14_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG04254.1"
                     /translation="MLGLSADLIRVVAEQGYSEPTPVQAQAIPVILEGRDVLAGAQTG
                     TGKTAGFALPLLQRLAGSGTAAAHSPRRVRALILTPTRELAAQIEESVRTYGKYLPLK
                     STLVYGGVNINPQIDALRRGVDILVATPGRLLDHLQQKTANLAYVEILVLDEADRMLD
                     MGFIRDIRRILALLPARRQNLLFSATFSNEIRKLADGLLHAPAHVEVARRNAESELVV
                     QRVHPVDGWRKRELLTHLVSAGDWRQVLVFTRMKSGANRLAEQLCKAGIEATAIHGNK
                     SQPARTRALAGFKAGTVRVLVATDIAARGLDIEELPHVVNFELPHVPEDYVHRIGRTG
                     RAGSAGEAISLVAVEENSLLADIEKLLTRRIERQVVEGFEPGQAPPPQVLAAAATQPT
                     GRGGRDHNRHSVGHAKDGLVRNPSVATLARPSTPASTPPRSSPPPQRSRPDTGAPLPA
                     LFRSPARRGG"
     gene            complement(1860..>2047)
                     /locus_tag="PLL14_09765"
     CDS             complement(1860..>2047)
                     /locus_tag="PLL14_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04255.1"
                     /translation="QAGKMDLHGKSPLQRSNFFLSWEYRKKNCGIPEKSNLIQASIPG
                     GVQPQASRCTDQSTRRG"
BASE COUNT          380 a          639 c          635 g          393 t
ORIGIN      
        1 ccttttgatg cgccgctgaa caattcgcgc atcacctgca ccgacttgac ctgtccgaag
       61 gccttgaaca gatcggtgag ttctgtttcg tccgtctcaa gcgcaagatt acctacgaag
      121 atgttcattg ccgattcctc tgatcagacg tttcgatacg tggggtgaag cgtggccttt
      181 gctcaaccgc cgcgccgcgc cgggctacgg aacagcgccg gcaacggcgc gccggtgtcc
      241 ggcctggaac gctgcggcgg cggactgctg cgcggcgggg tagacgccgg cgtgcttggt
      301 cgcgccaaag ttgccacgct cgggttacga accagaccat ccttagcgtg gccaacactg
      361 tgccggttat ggtcacgacc gccgcggccc gtgggttgtg tcgccgccgc ggccagcacc
      421 tgcggcggcg gcgcctggcc aggctcgaat ccctcgacca cttggcgttc gatacggcgg
      481 gtaagcagct tctcgatgtc ggccagcaag gagttctcct ccactgcaac gagggaaatc
      541 gcctcgccgg cactccccgc ccgtcccgtg cgtccgatcc gatgcacata gtcctcggga
      601 acgtgcggca gttcaaagtt gacgacgtgc ggcaactctt caatgtccag accacgtgcg
      661 gcaatgtccg tcgccaccag cacgcgtacc gtgcccgcct tgaagcccgc caatgcgcga
      721 gtgcgcgccg gctggctctt gttgccgtga atcgccgtag cctcaatgcc ggccttgcac
      781 agttgctccg cgagacgatt ggcaccgctc ttcatgcgcg tgaacaccag cacctggcgc
      841 cagtcacccg ccgacaccag gtgcgtcagc agttcgcgct tgcgccagcc gtcaaccggg
      901 tgtacccgct gcacgacgag ttcggactcg gcgttgcgac gtgcgacctc cacgtgcgcg
      961 ggggcatgca gcaggccatc ggcaagcttg cgaatctcat tggaaaaagt cgccgagaac
     1021 agcaggttct ggcgccgcgc cggcagcagc gcgagaatgc gtcggatatc acggatgaaa
     1081 cccatgtcga gcatgcggtc ggcttcgtca agcaccagga tctcgacata cgcgaggttc
     1141 gctgtcttct gttgcaagtg atcgaggagc cggccgggag tggccaccag gatgtccacc
     1201 ccgcgccgca gagcgtcgat ctgcgggttg atgttgaccc caccgtagac cagggttgat
     1261 ttgagcggca gatacttccc ataggtacgc acactttctt cgatctgggc cgccagttcg
     1321 cgtgttggcg taaggatcag cgcccgcacg cggcgggggg aatgcgccgc ggcagttccc
     1381 gatccagcca ggcgctgcag caacggtagt gcaaagccgg cggtcttgcc ggtaccggtc
     1441 tgagccccgg cgaggacatc acgtccttcg aggatgaccg gaatggcctg tgcctgtacg
     1501 ggggttggtt cgctgtagcc ctgttcggca acgacgcgaa tgaggtcggc cgacaggccg
     1561 agcatggcaa acgattggga caagaatacc tcctgcgaac gagcggctgt ggcacgacgc
     1621 catcagagcc ggctcgggcg gatacgacga cttttgggat tggacggttc ggggcgcagt
     1681 caggacactg ccgggggtga acagtcgcga aggaaacaaa ccgttggccg gccgacgcgc
     1741 aaactgcttc ctgtttggac tatacacggt ttcgggatac aggacaggaa tatcctgcta
     1801 caggttctgg tatggcctgg cgccagcctg agcctgcctg accccgccgc gatcgccaac
     1861 tatccgcgac gcgtcgattg gtcagtgcac cggctggcct gcggctgcac accaccaggg
     1921 atacttgcct gaattaaatt cgatttctct ggaattccgc aattcttctt tctatactcc
     1981 caggacaaga aaaaatttga tctttgcaaa ggagacttgc catgaagatc catttttccg
     2041 gcttgct
//
LOCUS       DAOSIT010000445         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47419_length_2045_cov_12.102513, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000445 DAOSIT010000000
VERSION     DAOSIT010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47419_length_2045_cov_12.102513"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PLL14_09770"
     CDS             complement(<1..1248)
                     /locus_tag="PLL14_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG04256.1"
                     /translation="MTQLRSRPVSQRVQWQLEQQGLHPLLARVYAGRGIVARSELDYE
                     FSSLLPPARLTHAAAAAVILADAIADRQRILIVADYDCDGATACALGIRALRTCGAAV
                     DYLVPNRFTYGYGLSPDIVDLAASRKPALIVTVDNGIASVDGVARARQLGIATLITDH
                     HLPGDELPAADCIVNPNLPACAFPSKCLAGVGVMFYVMLALRAELRSRNWFGAGSGRS
                     EPNLANLLDLVALGTVADVVSLDHNNRVLVSQGLQRIRQGRLTPGLRALFGAAGRNPA
                     QASSFDLGFMIGPRLNAAGRLSDMSLGIECLITDDQGRALHIAQQLDALNRERREIEA
                     GMQEQALLQVAKAAADLEGEPAAAVSLFDPDWHQGVVGIVAARIKERLHRPVFAFARG
                     DAGEIKGSGRSIPGLHLRDALDLV"
     gene            complement(1245..>2045)
                     /locus_tag="PLL14_09775"
     CDS             complement(1245..>2045)
                     /locus_tag="PLL14_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04257.1"
                     /translation="PVHLRLHQERLILADGGSLGIQSAEAQAIVDELNRQFSDRGTFH
                     ATAADRWYLQLPADTALGCFDVLPLSAVAGRSVGQQLPATPEARWLRQMLNEAQMILH
                     QHPANARREDEGRSTINSLWMWGAGKLPTGGAGEFAGLWSDQPLARGLGRAAAIPVQG
                     VPENAAALLARSPPGSRQMLLLDALQSPVQYEDSEAYRATLLDLEARWFAPLQKALAG
                     GRMQRLRLVATTAYATLAWDTDRLVQWQLWRRPQPLAATAQALARGER"
BASE COUNT          311 a          656 c          755 g          323 t
ORIGIN      
        1 caccagatcg agcgcatcgc gcagatgcag gccgggaatc gaacgtcccg agcccttgat
       61 ttcgccggca tcgccacggg caaaggcgaa caccgggcgg tgcagtcgct ccttgatgcg
      121 cgcggcgacg atgccgacga cgccctggtg ccagtcaggg tcgaacagcg acaccgcggc
      181 agcgggttca ccctccaggt cggcggctgc cttggcgacc tgcagcagcg cctgttcctg
      241 catgccggct tcgatttcgc gtcgctcacg attgagcgcg tcgagttgct gtgcgatgtg
      301 cagggcacgt ccttggtcgt cggtgatcag gcattcgatg cccagagaca tgtcggacag
      361 gcggccggcg gcgttgaggc gcgggccgat catgaacccg aggtcgaagc tcgacgcttg
      421 cgcgggattg cgacccgcgg cgccgaagag ggcgcgcaga cccggcgtca atcggccctg
      481 ccggatgcgc tgcagaccct ggctgacgag gacgcggttg ttgtggtcga ggctgacgac
      541 gtcggcgacg gtgccgagcg ccaccaggtc gagcaggttg gcgagattcg gctcgcttcg
      601 cccgctgccc gcgccaaacc agttgcggct gcgcaactcg gcgcgcagcg cgagcatcac
      661 gtagaacatc acgccgacgc cggccagaca cttgctcgga aaggcgcagg ccgggaggtt
      721 cgggttgacg atgcagtcgg ctgccggcaa ctcgtcgccc ggcaggtggt ggtcggtgat
      781 cagcgtggcg atgccgagtt gtcgggcgcg cgccacgccg tcgacgctgg cgatgccgtt
      841 gtcgacggtg acgatcagcg ccggttttct gctcgccgcg aggtcgacga tgtcgggcga
      901 caggccgtag ccgtaggtga agcggttcgg taccagataa tcgacggccg caccacaggt
      961 gcgtagcgcc cggatgccga gcgcgcaggc ggtggcgccg tcgcagtcgt agtcggcaac
     1021 gatcaggatg cgctgccggt cggcgatggc gtcggccagg atgacggcgg ctgcggcggc
     1081 gtgtgtcagg cgagccggcg gcagtagcga cgagaattca tagtcgagtt cgctgcgtgc
     1141 gacgatgccg cggccggcgt agacgcgcgc cagcagcggg tgcagaccct gctgttcgag
     1201 ttgccactgg acacgttgcg agacggggcg gctgcgcagc tgggtcatcg ctcgcctctg
     1261 gccagcgcct gcgcggtggc cgccagtggc tgcgggcggc gccacagttg ccactgtacc
     1321 aggcgatcgg tatcccaggc cagcgtggcg taagccgtcg tcgccaccag gcgcaggcgc
     1381 tgcatccggc cgccggcgag cgctttctgc agcggcgcga accagcgggc ttcgaggtcg
     1441 agcaaggtgg cgcggtaggc ctcgctgtct tcatactgca ccggcgattg cagtgcatcg
     1501 agcagcagca tctggcggct gccgggaggc gaccgagcga gcagcgccgc ggcgttttcg
     1561 ggcacgccct gcaccggtat ggccgcagcg cggcccagcc cgcgcgccag tggctggtcg
     1621 ctccacaggc cggcaaattc ccccgcgccg ccggtcggca gcttgccggc accccacatc
     1681 cacaggctgt tgatcgtcga tcggccttcg tcttcgcgcc tggcgttggc cggatgctgg
     1741 tgcaggatca tctgcgcctc gttgagcatc tggcgcaacc agcgtgcctc gggggttgcg
     1801 ggtaattgtt ggccgacgct gcgaccggcg acggccgaca gcggcaggac atcgaagcag
     1861 ccgagtgcag tgtcggcagg cagttgcagg taccagcgat cggcggcggt ggcgtgaaag
     1921 gtcccgcgat ccgagaactg gcgattgagt tcgtcgacga tggcctgcgc ctccgcggat
     1981 tgaatcccga gactgccgcc gtcggcgaga atcagtcgct cctggtgcag gcgcagatgc
     2041 accgg
//
LOCUS       DAOSIT010000446         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47471_length_2043_cov_7.600101, whole genome shotgun sequence.
ACCESSION   DAOSIT010000446 DAOSIT010000000
VERSION     DAOSIT010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47471_length_2043_cov_7.600101"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLL14_09780"
     CDS             complement(<1..937)
                     /locus_tag="PLL14_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOG04258.1"
                     /translation="MSFSTHPLWLVGFRPFFTLACLSGLSLPVLWALIFSGMLAPPAT
                     PFSPAQWHAHEMFFGFGWAVLGGFLLTATRNWVKIRGYHGQALMLLVSAWLLERAGMW
                     YAGILPPLVFRLFSNLFLAMIVLMLLWTLIRHHQDDSFPDNTFFLIVLPLFLVAKNLL
                     LSVDHFQVGAAMTLGLFRVAFLVMLERTVTQFMKNAVQADILRSAPLDRAIKLLALLL
                     VFVGLLPTQIAPWLALLLALLLSGRFVFWKPQLAMRRLDIGIMYLGYLAIVAQLLLQS
                     IEQLVTPGWLGSLSMHVFTFGAMGLIIPAMLIRIAN"
     gene            complement(1075..1767)
                     /locus_tag="PLL14_09785"
     CDS             complement(1075..1767)
                     /locus_tag="PLL14_09785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG04259.1"
                     /translation="MAPSGTARNVPPPPPYCPPPNDTLDLLYVDSWLLMANKPAGLLS
                     VPGRGEDKQDCLVLRVQAEYPDALIVHRLDLPTSGLLLLARGKEMQRRLSHEFAQRRV
                     EKRYLAVVMGCPLSVTGVIDLPLAVDWPNHPRQKVDRLTGKPALTRYRFLSHTASDDT
                     SRLALSPETGRTHQLRVHLQALGHPIVGDMLYADATTAAKSDRLLLHANELAFTHPAT
                     DRRMRFCSPPPF"
BASE COUNT          435 a          554 c          715 g          339 t
ORIGIN      
        1 cgttggcgat gcggatcagc atcgcgggga tgatcaggcc catggcgccg aaggtaaaca
       61 cgtgcattga caacgagcct agccatcccg gcgttacgag ctgctcgatg gactgcaaca
      121 gcaactgggc gacgatggcg agatagccga ggtacatgat gccgatatcg agtcggcgca
      181 tcgccagttg cggcttccag aagacgaagc ggccactcag caggagtgcc agcagcagtg
      241 ccagccacgg cgcaatctgt gtcggcagca ggccgacgaa cacgagcaat aaggccagca
      301 gtttgatcgc ccgatcaagt ggcgcgctgc gcaggatgtc ggcctgcacg gcgttcttca
      361 tgaactgtgt gacggttctt tcgagcatca ccaggaacgc gacgcggaac aatccaaggg
      421 tcatggcggc gccgacctgg aagtggtcta cgctgagcaa caggttcttg gcgaccagga
      481 acagtggcag gacgatcagg aaaaaggtgt tgtcggggaa ggaatcgtcc tggtggtgtc
      541 ggatcaacgt ccatagcagc atcaggacga tcattgccag gaagaggttg ctgaaaaggc
      601 ggaacacgag cggtggcaag attcctgcgt accacattcc agcgcgttcg agcagccagg
      661 cgctgaccag cagcatcagc gcctggccgt gatagccgcg aatcttcacc cagttcctgg
      721 tggcggtgag caggaagccg ccgagaaccg cccagccgaa gccgaagaac atttcgtggg
      781 cgtgccactg cgctggtgag aatggtgttg ctggcggtgc cagcattccc gagaagatca
      841 gcgcccagag tacgggcagg ctgagcccgg acaagcaggc gagggtaaag aagggtctga
      901 aaccgaccag ccatagcggg tgggtcgaaa agctcatgct cgcggggtct cggtcagata
      961 cggcgtggga aagcaggaag acagcagggg gcagatggct acccagtata gcgggatgac
     1021 cggagcctgt cgccagtcgg atccgcacgc cgccgtggta cagagccctg cgggctagaa
     1081 cggcggcgga ctgcagaaac gcatccgacg atccgttgcg ggatgcgtga aagccaattc
     1141 attggcgtgc agcagaaggc gatcggattt ggcggcggtc gtggcgtcgg cgtagagcat
     1201 gtccccgacg atggggtgcc cgagtgcctg caggtgcaca cgcaactggt gtgtgcgacc
     1261 ggtttcgggc gacagggcga ggcgcgaggt gtcatctgac gctgtgtgac tcaggaaccg
     1321 gtaacgcgtg agcgcgggct tgccagtcag tcggtcaacc ttctggcgtg gatgattcgg
     1381 ccagtcgaca gcgagtggca gatcaatgac accggtgacc gacaacggac accccatgac
     1441 gaccgccaga tagcgctttt cgacccgtcg ctgcgcgaat tcgtgactca gtcgccgctg
     1501 catttccttg ccacgcgcca gcaggagcag gcctgaagtc ggcaggtcaa ggcgatgcac
     1561 gatcagcgcg tcgggatact cggcctggac gcgcagcacc aggcagtcct gtttgtcttc
     1621 gccgcgaccg ggtaccgaga gcaggccggc cggtttattt gccatcagta gccaggagtc
     1681 gacgtacagg aggtccagcg tgtcgttggg tggcgggcag tagggtggcg ggggcggaac
     1741 attccgcgca gtgccggagg gtgccatggg cgaatcggga ctaggcccgg gaaacgagcg
     1801 gtgtggcccc gcaccatgat gacctggtat aaagggcgtt gacaaaggat atgatgggta
     1861 ttcggctggt gcaggggttg cagtcagcaa agaccaagta tagacattga ccggcggttg
     1921 cgcgggtgtc ggccgttcaa tcactttggc agcgcagcgc gatggcacgc aggaattcgc
     1981 tgcgcaattg cgcggagcgc tcgaactcgc cgctgaagga ctgggtgatc acgcgctcgt
     2041 cgc
//
LOCUS       DAOSIT010000447         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47588_length_2039_cov_5.040827, whole genome shotgun sequence.
ACCESSION   DAOSIT010000447 DAOSIT010000000
VERSION     DAOSIT010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47588_length_2039_cov_5.040827"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..703)
                     /locus_tag="PLL14_09790"
     CDS             complement(290..703)
                     /locus_tag="PLL14_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04260.1"
                     /translation="MSIWILFPFAFVGASLWLWWKRTRRVPSPVPGEEHAHPFQPYHC
                     VSIKPHDEACAGARLIEGRRFLPYAAPLLPMNVCDSSSCRCRYAHYDDRRHDERRGWN
                     AILHGFAAAPGDVERRSCADRRRSPEYDPCTLQGT"
     gene            complement(763..1080)
                     /locus_tag="PLL14_09795"
     CDS             complement(763..1080)
                     /locus_tag="PLL14_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04261.1"
                     /translation="MTDSSLGALFPIDRQRILRRDRHRDPSLIACPGKIRETFPEMPG
                     VFPLPRKPDRVRKFGPNQGIAANTRIPARRMCTLEITRRVKLAMIDAPDGRFGASIGN
                     LRT"
     gene            1427..2032
                     /locus_tag="PLL14_09800"
     CDS             1427..2032
                     /locus_tag="PLL14_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOG04262.1"
                     /translation="MLLYYLDLAGVAVFAASGVLAARDRHLDLLGVIVVAAITAIGGG
                     TLRDLLLDRHPIFWVIDTWYLVVIIASALLTVAYVRIWPPPNATLLVADALGLALFAL
                     SGAQLAEAAQCPALIVVLMGTMTGVTGGVLRDVITAQVPLILRREIYATAAIVGVAVY
                     QTLHALGTPRAAAFWAGMIVVVAMRLLAIRWGLHLPVFRKF"
BASE COUNT          354 a          589 c          672 g          424 t
ORIGIN      
        1 tcgctggatt cccaagccgg taccggccac aatgattagc tgttgatgat attgatggcg
       61 tggctgtggg gaattgacgc gtgtcaattc tggaaaaagc agtccgcgcc gccgccaagg
      121 cagttttcag gcctttgcgg ctggcgcatg tttggtgcgg ggttgatacg gggttgatgc
      181 ggcgggaggc acctggcgga gccttccggg cgttccttcc tctgtggtca gagtgggccc
      241 cggatcaggc cggtgagcga ctcgccaagc ccgcgctcgg gtgcttgcct caggtcccct
      301 gcagtgtgca agggtcatac tccggggatc gtctgcggtc ggcgcaacta cggcgttcga
      361 catcacctgg agccgccgcg aagccgtgca ggatggcatt ccaaccgcgc cgctcgtcgt
      421 gacggcggtc atcgtagtgt gcgtagcggc accgacagct cgacgaatcg cagacattca
      481 tcggcagcag gggtgccgca tagggcagaa agcgcctgcc ctcgatcagc cgggctcccg
      541 cgcaggcttc gtcgtggggc ttgatcgaga cacagtgata cggctgaaaa gggtgtgcat
      601 gttcctcgcc aggtaccggg gatggcacgc gacgcgtgcg tttccaccac agccagagag
      661 atgcgccgac gaaagcaaag ggaaacagta tccagataga catgacatgg ccctcctgac
      721 gattgcctgg ttggtgatgc attggactcg tgtttgaccc atttacgtcc gcaaattccc
      781 gatggatgcg ccgaaacgac cgtccggagc gtcgatcatc gcgagtttca ctctgcgagt
      841 gatctcgagt gtgcacatcc tgcgcgcggg aattcgggta ttcgcggcaa tgccctgatt
      901 cggtccaaat tttcggacac ggtccggctt tctgggtagt ggaaaaactc ccggcatttc
      961 cgggaatgtt tcccggattt ttccagggca ggcgatcaat gacggatcgc gatggcgatc
     1021 ccggcgcaaa atccgctgac ggtcaatcgg gaatagtgca ccgagggaac tatcggtcat
     1081 cagccgacgg ctgcgatctt gccgcatagc cgacgttgac gcggagcgat cagcccgaag
     1141 cagtccattc gacaggcgat gtcaattttt tctcgccaga ccggtcgcca ggccgcggga
     1201 agcggccctt gcgtgtagga tagcgttagc cggcaaccgg ccaacacctg ccgttggggt
     1261 ctccgatgcg gacgttggtt caagcctggc aacggtcgat gggccgagat gcggcgctga
     1321 ccggttccat acaaaacacc ggatacgtga ctcgttcatc cggtgtggtg ggagggaggg
     1381 gtcgcgaggc ttcttccgat cccgattcgg caatgtagga cggtgcgtgc tcttgtatta
     1441 ccttgacctt gccggcgtgg ccgtgtttgc cgcgagtggc gtacttgcgg cgcgagatcg
     1501 gcatctggac ctcctcgggg tgatcgtggt cgcggcgatt acagccatcg gaggaggtac
     1561 gcttcgcgat ctcctgctgg atcgccaccc gatattctgg gtcatcgata cctggtacct
     1621 cgtcgtgatc atcgcctccg ccctgttgac ggtcgcgtat gtacggatat ggccaccgcc
     1681 gaacgccaca ctcctcgtgg ccgacgcgct gggactggcg cttttcgcgc tttccggggc
     1741 ccagttggcc gaagcggcgc agtgcccagc gctcatcgtg gtgctgatgg gcacgatgac
     1801 gggcgttacc ggaggggtct tgagggacgt catcacggcg caggtaccgc ttatcctgcg
     1861 gcgggagatc tatgcgacgg ccgccatcgt gggcgtcgcc gtgtaccaga cgttgcacgc
     1921 gctcgggacg ccacgtgcag cggcgttttg ggccggaatg atcgtggtcg tggcgatgcg
     1981 cttgcttgcc atccgatggg gcctgcacct acccgtcttc cgcaaattct agcgtcaaa
//
LOCUS       DAOSIT010000448         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47643_length_2037_cov_6.689203, whole genome shotgun sequence.
ACCESSION   DAOSIT010000448 DAOSIT010000000
VERSION     DAOSIT010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47643_length_2037_cov_6.689203"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLL14_09805"
     CDS             complement(<1..1306)
                     /locus_tag="PLL14_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HOG04263.1"
                     /translation="MPRRSILSATERESLLALPDAKDELIRHYTFNETDLSVIRQRRG
                     AANRLGFAVQLCYLRFPGIFLGIDEPPFPPLLRMVAAQLKVPVESWNDYGQREQTRRE
                     HLVELQTVFGFKPFTMSHYRQAVHTLTELALQTDKGIVLASTLVENLRRQSIILPAMN
                     AIERASAEAITRANRRIYAALTDSLLSLHRQRLDELLKRKDGSKLTWLAWLRQSPVKP
                     NSRHMLEHIERLKAWQALDLPAGIERQVHQNRLLKIAREGGQMTPADLAKFEMQRRYA
                     TLVSLAIEGMATVTDEIIDLHDRIIGKLFNAAKNKHQQQFQASGKAINDKVRMYGRIG
                     QALLEAKQSGGDPFAAIEAVMPWDTFAASVTEAQKLAQPESFDFLHRIGESYATLRRY
                     APQFLDVLKLRAAPAAKGVLDAIEVLRGMNSDNARKVPADAPT"
     gene            complement(1309..1866)
                     /locus_tag="PLL14_09810"
     CDS             complement(1309..1866)
                     /locus_tag="PLL14_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOG04264.1"
                     /translation="MQGQRIGYIRVSSFDQNPDRQLEQIEVGKVFTDKASGKDTQRPE
                     LERLLAFVREGDTVVVHSMDRLARNLDDLRRIVQGLTQRGVRMEFVKEGLAFTGDDSP
                     MANLMLSVMGAFAEFERALIRERQREGIVLAKQRGAYRGRKKSLNSEQIAKLKQRVAA
                     GDQKTLVARDFGISRETLYQYLRED"
BASE COUNT          379 a          599 c          604 g          455 t
ORIGIN      
        1 cggtcggcgc gtcggcgggc accttgcggg cgttgtcgct gttcatgccg cgaagcacct
       61 cgatggcatc gagtacgccc ttggcggctg gtgccgcccg taacttaagt acgtccagga
      121 actgcggcgc gtagcggcgc aacgtggcgt agctttcacc gatgcgatgc agaaagtcga
      181 agctctccgg ctgcgcgagc ttttgcgcct ccgtgacgct ggcggcgaag gtatcccacg
      241 gcattacggc ctcgatggcg gcgaacggat cgccgccgct ctgtttggct tccagcaagg
      301 cttggccgat gcgaccatac atccgcacct tgtcattgat cgccttgccg gaagcctgaa
      361 actgctgctg atgcttgttc ttggcggcgt tgaatagttt gccaatgatg cggtcgtgca
      421 ggtcgatgat ttcgtcggtg acggtggcca taccttcaat ggccagcgaa accagagtgg
      481 catagcgtcg ttgcatctcg aactttgcca gatcggcagg cgtcatctgc ccaccctcac
      541 gggcgatctt gagcaggcgg ttctggtgaa cttgtcgctc gatgcctgcg ggaaggtcaa
      601 gcgcctgcca agccttgagg cgctcgatgt gttcaagcat atgccgagag ttcggcttga
      661 cgggcgactg gcgcagccat gccagccacg tcaatttgct gccgtccttg cgcttgagaa
      721 gttcgtccag gcgctgacgg tggagcgata acaaagaatc ggtcaacgcc gcgtaaatac
      781 gccggttggc acgggtgatg gcctcggcgc tcgcgcgctc gatggcattc atggcgggca
      841 ggatgatgct ctgccgccgc aggttttcga caagggtgct ggccagcaca atccctttat
      901 cggtctgcaa ggccagctcg gtcaatgtat gcacggcttg ccggtagtga ctcatggtga
      961 agggcttgaa cccgaacacc gtttgcagct cgaccaagtg ctcccgccgc gtctgctcgc
     1021 gctggccgta atcgttccaa ctttccaccg gcaccttgag ttgtgcggcc accatgcgca
     1081 acaggggcgg aaacggaggc tcatcgatgc ccaagaagat gccagggaat cgcaagtagc
     1141 aaagctgcac ggcgaagccc aatcgattcg cagcgccgcg acgctggcgg atcaccgata
     1201 ggtcggtttc gttgaacgtg tagtgccgta tcagttcgtc tttggcatct ggtagtgcca
     1261 gcaggctttc gcgctcggtg gcggacagga ttgagcggcg cggcatgatc agtcttcccg
     1321 caggtactgg tacaaggttt cgcgactgat gccgaagtca cgggccacca aggttttttg
     1381 atcgcctgcc gcgactcgct gtttcaactt ggcaatttgt tcgctgttca gcgatttctt
     1441 tcgtccccgg taggcaccgc gctgcttggc cagcacgatt ccctcgcgct ggcgttcgcg
     1501 gatcagtgcg cgctcgaact ccgcaaaagc ccccatgacc gacagcatca aattggccat
     1561 cggtgagtca tcgccggtga acgccagccc ttctttgaca aactccatcc gcacgccccg
     1621 ttgtgtcagc ccctgaacga tgcggcgcag gtcatcgaga ttgcgtgcca acctgtccat
     1681 gctgtgcacc accacggtgt cgccctcgcg cacaaaggcc agcagccttt caagttcggg
     1741 acgttgcgtg tccttgccgg aagccttatc ggtgaatacc ttaccgactt cgatttgctc
     1801 cagttgccga tcagggttct ggtcgaagct gctgacgcgg atatagccaa tgcgttgacc
     1861 ttgcaaggtg cctccaaagg taaaagtgtc aggatgaaat ccattaccct tggcggcttg
     1921 tgtcaataaa tacaaactac aactctattc tgacgtgctt tgcgccaagc atctgacatc
     1981 aggttagggt atagcttaac cggacacatc ggcctctgtg cctctactgc ttttcgg
//
LOCUS       DAOSIT010000449         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47802_length_2032_cov_11.056146, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000449 DAOSIT010000000
VERSION     DAOSIT010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47802_length_2032_cov_11.056146"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLL14_09815"
     CDS             <1..342
                     /locus_tag="PLL14_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOG04265.1"
                     /translation="GVGIGGVLEPLQANPTIIEDDCFIGARSEVVEGVIVGAGSVISM
                     GVYLGQSTKIFDRETGIVSYGRVPAGSVVVAGNLPAKDGNYSLYCAVIVKKVDAGTRA
                     KTSINDLLRGD"
     gene            380..1561
                     /locus_tag="PLL14_09820"
     CDS             380..1561
                     /locus_tag="PLL14_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG04266.1"
                     /translation="MILDKLFQVMADRQASDIFICAGAPIHIKIQGNTVPVNQQVMDS
                     MMIEKIAAELMTPEQANAFEATKEMNLSFGVPKLGNFRINLFQQRASISIVIRYILGN
                     IPALDSLLLPPVLADLVMEKRGLILIVGSTGSGKSTTIASMLDHRNAQCSGHILTIED
                     PIEYLFKHKKSLVNQREIGMDTLGWDNALKNAMRQAPDCILIGEIRDQETMQAAIAYA
                     QTGHLCLGTLHANNSYHALNRVINFFPIDSRSSLYMDLSVSLKAIISQRLVKKKDGKL
                     APAVEVLLNSYHVQELIESGNVPALKEAMEQSLAPGSQTFEQDLYRLYSSNVITLQEA
                     LLNADSPTNLSWMINNHQAPPTPVTREKVTDIGLDFSATNPGGPSYKEFTISLDEADG
                     Q"
     gene            1576..>2032
                     /locus_tag="PLL14_09825"
     CDS             1576..>2032
                     /locus_tag="PLL14_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG04267.1"
                     /translation="MPTERSSNTPRDATLLLTEQLMACRSVTPDDAGCLQIIAGRLEA
                     IGFACEFMPRGGVNNLWARRGEARPLLCFAGHTDVVPSGPPQEWRSDPFQPVYRDGML
                     YGRGAADMKSSLAAFITAAEAFFAARPEPAGSLAFLLTSDEEGDATDGTV"
BASE COUNT          411 a          694 c          590 g          337 t
ORIGIN      
        1 ggggtcggca tcggcggcgt gctcgagccg ctgcaggcca acccgacgat catcgaggac
       61 gactgcttca tcggcgcccg ctcggaagtc gtcgaggggg tcatcgtcgg tgccggttcg
      121 gtgatctcga tgggcgtcta tctcggtcaa tcgaccaaga ttttcgaccg cgagaccggt
      181 attgtgagct atggccgcgt gccggcgggt tcggtcgtgg tcgccggcaa cctgcccgcc
      241 aaagacggca actacagcct gtactgcgcg gtcatcgtca agaaggtaga cgccggaacg
      301 cgcgccaaga cgagcatcaa cgatctcctg cgtggcgact gagcgcatcc ccgggtagcc
      361 ctgaaacggc ggcaaacgca tgatcctaga caagcttttt caggtgatgg ccgatcgaca
      421 ggcgtcggac atcttcatct gcgccggtgc gccgatccac atcaagatcc agggcaacac
      481 cgtgccggtg aatcagcagg tcatggattc gatgatgatc gagaagatcg ccgccgaact
      541 gatgactccc gagcaggcca acgccttcga ggcgacgaag gagatgaacc tctcgttcgg
      601 cgtacccaag ctcggcaatt tccgcatcaa cctcttccag cagcgcgcct cgatatcgat
      661 cgtgatccgc tacatcctgg gcaacatccc ggcgctcgat tcgctgctgc tgccgccggt
      721 gctggccgac ctggtgatgg aaaaacgcgg cctgatcctg attgtcggat cgacgggctc
      781 gggcaaatcg accaccatcg cgtcgatgct cgaccaccgc aacgcgcagt gcagcggcca
      841 catcctcacc atcgaggatc cgatcgaata tctgttcaag cacaagaagt cgctggtcaa
      901 ccagcgcgag atcggcatgg acaccctggg ctgggacaac gcgctgaaaa acgccatgcg
      961 gcaggcgccc gactgcatcc tgatcggcga aatccgtgac caggaaacga tgcaggcggc
     1021 catcgcctac gcccagaccg gccacctctg tctcggcacg ctgcacgcca acaacagcta
     1081 tcacgcgctc aaccgggtga tcaacttctt cccgatcgac agccggagtt cgctgtacat
     1141 ggacctctcg gtcagcctga aggcgatcat ttcgcaacgt ctggtgaaga agaaggacgg
     1201 caagctcgca ccggccgtcg aagtcctgct gaacagctat cacgtgcagg aactgatcga
     1261 gagcggcaac gtcccggccc tcaaggaagc gatggaacag agcctcgctc ccggatcgca
     1321 gaccttcgag caggatctct accgcctcta cagctcgaac gtgatcaccc tgcaggaagc
     1381 cttgctcaat gccgactcac cgaccaacct gtcgtggatg atcaacaacc accaggcgcc
     1441 gcctaccccg gtgacgcggg aaaaagtgac cgacatcgga ctcgacttct cggcgaccaa
     1501 ccctggcgga ccgtcgtaca aggagttcac catcagcctc gacgaggccg acggccagta
     1561 gccgggaacc agcagatgcc caccgagcgc tcctcgaaca cgccacgcga tgccacgctc
     1621 ctcctgaccg agcaactgat ggcctgccgc tcggtcaccc ccgacgacgc cggttgcctg
     1681 cagatcatcg ccggtcgtct cgaagccatc ggtttcgcct gcgagttcat gcctcgcggc
     1741 ggcgtcaaca acctctgggc acgccgtggc gaggcccgtc cgctgctgtg cttcgccggc
     1801 cataccgacg tcgtccccag cggcccgccg caggaatggc gcagcgaccc ctttcaaccc
     1861 gtctaccggg acggcatgtt gtatggacgc ggcgccgctg acatgaagag ttccctcgcc
     1921 gccttcatca ccgcggccga agccttcttc gccgcgcgtc ccgaaccggc gggctcgctc
     1981 gccttcctgc tcacttccga cgaggaaggc gacgccaccg acggcaccgt cg
//
LOCUS       DAOSIT010000450         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47835_length_2031_cov_6.798583, whole genome shotgun sequence.
ACCESSION   DAOSIT010000450 DAOSIT010000000
VERSION     DAOSIT010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47835_length_2031_cov_6.798583"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLL14_09830"
     CDS             <1..409
                     /locus_tag="PLL14_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04268.1"
                     /translation="RPQNVGVIGVAVYREAEATPPPAVAMPSRERDAPAAAAGAVANA
                     AAKAAAPAAPPARDEITTRQIESRLGTGHGERINAPTQLTEFRRATDSAAEILTIFYD
                     SYANLLAQGIIPRPKREPAPNPFPGGFAPDPRR"
     gene            498..>2031
                     /gene="leuS"
                     /locus_tag="PLL14_09835"
     CDS             498..>2031
                     /gene="leuS"
                     /locus_tag="PLL14_09835"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG04269.1"
                     /translation="MQEKYQPAELEVAAQELWNRSGAACAIEDPSRPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRFHRMLGYNVLQPMGWDAFGMPAENAAIQNNVPPAQWTY
                     ANIAYMKTQLKRLGFAIDWQRELATCTPEYYRWEQWLFTRLYAKGLIYQRRGTVNWDP
                     VDQTVLANEQVIEGRGWRSGALVEKREIPMYYMKITAYAEELLADLDQLDGWPEQVRL
                     MQKNWIGKSTGVRFAFPYELDGQPGQLWVFTTRADTIMGVTFCAVAAEHPLASYAAAR
                     DPQVAAFVEECKRGGVAEADLATMEKKGLPTGIFVTHPLSGEQVEVWIGNYVLMSYGD
                     GAVMAVPAHDERDFVFARKYGLPIRQVIAVPGESFSLEGWQEWYADKQRGVCVNSGKY
                     DGLGCAAAVDAIAADLAAKGLGEKKVQFRLRDWGISRQRYWGCPIPIIHCATCGDVPV
                     PDADLPVVLPEDVTITGAGSPLARLPEFYETTCPGCGGPARRETDTLDTFVESSWYFL
                     RYC"
BASE COUNT          374 a          663 c          651 g          343 t
ORIGIN      
        1 tcgcccacag aacgtcggcg tgattggcgt tgccgtctac cgggaagccg aggccacgcc
       61 gccgccggcg gtggcgatgc cgtcgcgtga gcgcgatgcg cctgctgctg ccgcgggggc
      121 ggtggcgaac gcagcggcca aggctgccgc cccggctgct ccaccggcgc gtgacgaaat
      181 caccaccaga cagatcgaaa gtcgtctcgg taccggccac ggcgaacgca tcaatgcacc
      241 gacgcagctc accgagtttc gccgcgccac cgactcggcg gccgagattc tcaccatctt
      301 ttacgacagc tacgccaacc ttctcgccca gggcatcatt cctcgaccaa aacgcgaacc
      361 ggccccaaat ccctttccgg gtggcttcgc tcccgatccg cgtcgctgag gaggcaacac
      421 tcggctgccg gcggcgcggc gtcaggcgcg tatgcgtata attcgcgact tggcccattt
      481 ccggatcgta accaaccatg caggaaaagt atcaacccgc ggagcttgaa gtcgccgccc
      541 aggaactctg gaaccgcagc ggcgccgcgt gcgccatcga ggacccgagc cggcccaagt
      601 actactgcct gtcgatgttc ccctatccct cgggcaagct gcacatgggg cacgtgcgca
      661 actacacgat cggcgacgtc ctcactcgct tccaccggat gctgggctac aacgtcctgc
      721 agccgatggg ttgggacgct ttcggcatgc cggcggaaaa cgccgcgatc cagaacaatg
      781 tgccgccggc gcaatggacc tacgccaaca tcgcgtacat gaagacccag ctcaagcggc
      841 tgggctttgc catcgactgg cagcgtgaac tcgccacctg tacgccggag tactaccgct
      901 gggaacagtg gctatttacc cgcctgtatg ccaagggctt gatctaccag cgtcggggaa
      961 ccgtcaactg ggatccggta gaccagaccg tgctggccaa cgagcaggtc atcgagggcc
     1021 gcggctggcg ttcgggcgcg ctggtcgaga agcgcgagat cccgatgtac tacatgaaga
     1081 tcaccgccta cgcggaagaa ctgctcgccg acctcgatca actcgatggc tggccggagc
     1141 aggtgcgcct gatgcagaag aactggatcg gcaagagtac cggtgtgcgc ttcgcctttc
     1201 cctatgagct cgatggccag cccggccagt tgtgggtatt caccacgcgc gccgacacga
     1261 tcatgggcgt caccttctgc gccgtcgccg ccgaacatcc gctggccagt tacgcggcgg
     1321 cgcgtgaccc gcaggtggcg gcttttgtcg aagagtgcaa gcgcggtggc gtcgccgagg
     1381 cggacctcgc gacgatggag aagaagggcc tgccgaccgg cattttcgtc acccacccgt
     1441 tgagcggcga acaggttgaa gtatggatcg gcaattacgt gctgatgagc tacggcgacg
     1501 gtgcagtgat ggcggtgccg gcgcacgacg agcgcgactt cgtatttgcc aggaaatacg
     1561 gattgccgat caggcaggtg atcgccgtgc cgggcgaaag cttttcactc gaaggctggc
     1621 aggagtggta cgccgacaaa cagcgcggcg tctgcgtcaa ctccgggaaa tacgacggtt
     1681 tgggctgtgc ggcggcggtt gacgccatcg ccgcggatct cgcggccaaa ggtctggggg
     1741 aaaagaaggt gcagttccgc ctgcgcgact ggggcatttc ccggcagcgc tactggggct
     1801 gcccgatccc gatcatccac tgcgcgacct gcggcgacgt gccggtgcct gacgcagacc
     1861 tgccggtggt gctgccggaa gacgtcacca tcaccggcgc cggctcgccg ctggccagac
     1921 tgcccgagtt ttacgaaacc acctgccccg gatgcggtgg cccggcccgg cgcgagaccg
     1981 atacgctgga cacctttgtc gagtcctcgt ggtacttcct gcgttattgc t
//
LOCUS       DAOSIT010000451         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47928_length_2028_cov_9.760264, whole genome shotgun sequence.
ACCESSION   DAOSIT010000451 DAOSIT010000000
VERSION     DAOSIT010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47928_length_2028_cov_9.760264"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..2020
                     /locus_tag="PLL14_09840"
     CDS             356..2020
                     /locus_tag="PLL14_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04270.1"
                     /translation="MYEALRAYFLEDQPSQQVARNFGYSPGAFRVLCHQFRRDPDPQF
                     FVTPSHGPRSQPKKSSARELAVMLRKQNHSVYEIAQALKERQMALSPTAVRELLRAEG
                     FAPLPRRLDEERPTCIAPTVEPVADARAFSLVPGTQFVTRCGGLFLFLPELVRLQVDT
                     LANAARLPGSVMIPSGHALRAALALKLWSIERKSHVMALVADPGLALFCGLNAIPKKS
                     YLCEYSSRLDHARTTALLAAWHRTVAQDNLFAGESFNLDFHSVPYYGEDPQLERHYVS
                     MRSRAQPSVLAFLAQDAAGRAFCYSNADLRKGEEAEEIFHFIAFWKKHHGENPRHLVF
                     DSRLTTYGHLARLEALDITFITLRRRSAKLLAEIKGLPASSWRRVKLDVPARKYKTPQ
                     VFEQPVTLAGATFRQLFVLDLGHEEPTILLTNDRHSTPAKLLTRYAQRMLIENALSDA
                     VRFFHMDALSSAVGLKVDFDMALLVIASGLYRLLAQRMRGYADAQARHIFRDLIDTPA
                     DVTVSEREVHVRFHRRAHLPIIIASGILDSQVQVPWWQDYTLSMSS"
BASE COUNT          372 a          657 c          553 g          446 t
ORIGIN      
        1 agcctggatt tccacggacc agagttggct aacgcggttc ggtgcgcagg ctggctccgc
       61 gcaccggaaa tgagtggatg cggttctact tgcgttagct aaaactgcac cgacactccc
      121 atatttcaac ctacataccg ctaccgtcat atctcaagca tttctatgct ggggatagcg
      181 actgcttgca tttctcttaa ccacacggtt ttcagcttac tacctcttgt gggttaaaaa
      241 acccttgaca agttgtaatg acatgtgcaa ctatgcattt atggctatag gcgtacctaa
      301 agacataacg caatgccgca gtttcttcgc ggagccttcc caaccgcgcc agcgtatgta
      361 cgaggccctg cgcgcctact tcctcgaaga tcagccctcc caacaggtcg cccgcaattt
      421 cggctatagc cccggcgcgt ttcgggtgct ttgccatcag ttccgcaggg atccggatcc
      481 gcagttcttc gttaccccat cgcatggtcc ccgctctcaa ccgaagaagt cctcggcgcg
      541 ggaactcgcc gtgatgctgc gcaaacagaa ccattcggtc tacgagatcg cccaagcgct
      601 gaaggaaagg cagatggcct taagcccgac ggcggtgcgg gagctgctgc gcgcagaagg
      661 cttcgccccc ttgccgcgac gcctggacga ggaacggcca acgtgcatcg cgcccaccgt
      721 ggaaccggtg gccgatgcgc gggccttctc tctggttccc ggcacccagt tcgtgacccg
      781 ctgcggcgga ctcttcctgt tcttgccgga acttgtacgc cttcaggtgg acaccctggc
      841 caatgcggct cgcctgccgg gctcggtcat gattccgtcg ggacacgcgc tgcgcgccgc
      901 tttggcactg aagttgtggt ctatcgaacg taagagccat gtgatggcac tcgtcgccga
      961 tcccggtctg gcgctgttct gcggtcttaa cgcgatcccg aagaagagct acctgtgcga
     1021 gtattcctcc cgcctggatc acgctcgcac caccgccctg ctggcggcct ggcaccgcac
     1081 ggtggcgcag gacaatctct tcgccggtga atcgttcaat ctcgatttcc attcggttcc
     1141 ttactacggc gaggatccgc agctcgaacg tcactatgtt tcgatgcgca gccgtgccca
     1201 gcccagcgtt ctggccttcc tcgctcagga tgcggcgggt cgggcgttct gctactccaa
     1261 cgccgatctg cgtaaggggg aagaagcgga ggaaatcttc cacttcatcg ctttctggaa
     1321 gaagcaccac ggcgagaatc cccggcatct cgtctttgat tcgcgcctga ccacctacgg
     1381 gcatttggcg cgactggagg ccttggatat cactttcatc acgctgcgtc ggcgatccgc
     1441 caagctgctc gccgaaatca agggactacc ggcttccagt tggcgtcgcg tgaagctgga
     1501 tgtgccagcg cgcaagtaca agacgccgca ggtgttcgag cagccggtca ccttggccgg
     1561 agccacattc cggcaactgt tcgtgctcga tctgggtcac gaggagccga ccatcctgct
     1621 caccaacgat cgccacagca ctcccgccaa gctgctgacc cgctatgctc agcggatgct
     1681 catcgagaac gccctgtccg atgccgtgcg cttcttccat atggatgcac tctcttctgc
     1741 agtcggtctc aaggtcgatt ttgatatggc cctgctggtc attgccagcg gtctgtatcg
     1801 tctgctggct cagcgcatgc gtggttacgc cgacgcccag gcacgccaca tcttccgcga
     1861 cctcatcgat acccccgccg atgtcactgt ttccgagcgc gaggtccatg tgcgcttcca
     1921 tcgtcgcgct cacctgccga tcatcatcgc ctcaggtata ctcgactcac aggtacaggt
     1981 gccctggtgg caggattaca ccctgagcat gagttcgtag ctccaaca
//
LOCUS       DAOSIT010000452         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47934_length_2028_cov_7.028890, whole genome shotgun sequence.
ACCESSION   DAOSIT010000452 DAOSIT010000000
VERSION     DAOSIT010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47934_length_2028_cov_7.028890"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(522..1238)
                     /locus_tag="PLL14_09845"
     CDS             complement(522..1238)
                     /locus_tag="PLL14_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOG04271.1"
                     /translation="MASHSVAEQTRRALEVHLLDGSWPVGMRLPAERVLAETLGVSRN
                     SVREAIFCLKERGLLFSRHGSGVFVSDRLQTAISSPWRQLVAAHPDLRWDTLEFRREL
                     EGATAYYAALRANASDLENIEGIVRRLTDAYDTGEKEEEYRADADFHEAIAEASHNSM
                     FLYLHSGIVRMLREHICLNLMAMEDPTGDVTEHLRQQHLGIWERIRTQQPDEARQAML
                     AHIDFTWSELARREDAGASR"
     gene            1446..>2028
                     /locus_tag="PLL14_09850"
     CDS             1446..>2028
                     /locus_tag="PLL14_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG04272.1"
                     /translation="MNSLISALRTHFPATSIITDELRLLAWGTDASFYRLIPQVVVVV
                     ENEDDVAHVLAVAAQQGVHLTFRAAGTSLSGQAVTDGILVLIGEAFATCEIGPDAATV
                     RVGPGIIGGEVNYRLAPHGRKIGPDPASISACKMGGIAANNASGMCCGTAQNSYRTLA
                     GLRVVFADGSVLDTEDAASVARFRASHAALVDEL"
BASE COUNT          361 a          652 c          612 g          403 t
ORIGIN      
        1 tatcacgcag tcattgagga aagcctgaaa cagggacagg tttcgggctt tcctggtgtc
       61 gaccgcgaca ctgcatgacg tgtgtccggc tttatggacg cctggcgacg atcctggccg
      121 ctgcacttcc gaatagccat atggctggct acctcggcgt aaaccaggag gcggccgatc
      181 agaacagggc ggagcaggag gcatggatga gtttttcttg gtcatcgcat gagaaacgat
      241 cgtttgccgc gcgctacgct gccctatatc gtggcgatat tccctcacga agggccatct
      301 caagcgccag gagtcgacat catgaaccat cccgatacac tgtccgtagt ccagtctcat
      361 ggtggccagg accggcgtgg cgccggcggc ccggttggtt cttcggcatg gcgggccctc
      421 tggaagcttt tcgccggact ttgcgagccg gcgcggacga cctgcaggaa cccagccgcc
      481 tatccggcga ccttcatcaa ccttgcctga tccagacacc gctagcgact ggcgcctgcg
      541 tcttcccggc gcgccagttc cgaccacgta aagtcgatgt gagcgagcat cgcctgcctg
      601 gcttcgtccg gctgctgcgt gcggatccgc tcccagatgc caaggtgctg ttggcgaagg
      661 tgttcggtga cgtctcccgt cggatcttcc atggccatca gattgaggca gatgtgctcg
      721 cgcagcatgc gcacgatgcc tgagtgcagg tagagaaaca tgctgttgtg cgaggcttcg
      781 gcgatggcct cgtggaagtc ggcgtcggcc cggtactctt cctccttttc gcccgtgtcg
      841 taggcgtcgg tcagacgcct gacgatgcct tcgatgttct ccagatcgct ggcgttggcg
      901 cgtagcgcgg cgtagtacgc ggtcgcaccc tccagttcgc ggcgaaactc cagcgtatcc
      961 caacgcaagt ccggatgcgc ggccaccagt tgccgccacg gtgacgagat tgccgtctgc
     1021 aggcggtcgc tgacgaaaac gccgctgccg tgcctgctga acagcaggcc gcgctccttc
     1081 aggcaaaaaa tcgcctcacg taccgagttc cgcgaaacgc ccagcgtctc cgccaggacc
     1141 cgctcggccg gcaaacgcat gccgaccggc caggaaccat ccagcaggtg tacttcgagt
     1201 gcccgccggg tctgttctgc aacgctgtgc gatgccatgg ccatcctccc tgaatgtcct
     1261 cgacgacctg cctggcgcca ataaacaatt ggtactacca atttccgttc aatgctatct
     1321 ctgattaatc ttcgcgccgt gggttttcgt taagtgcaac gtattaccat agagtgaatg
     1381 ccccgccggc gttgccgaga aaggggcgta accacccgtc gggtctctgg aggaggaagc
     1441 caagaatgaa ctcgctcatt tctgccctgc gcacgcattt ccccgcaaca agcatcatta
     1501 ccgatgaact gcgactactg gcctggggca ccgatgccag cttctaccgg ctgatcccgc
     1561 aggttgtggt cgtcgtggaa aacgaagacg atgtggcgca cgttcttgcg gtagccgcgc
     1621 agcagggtgt tcacctgacc ttccgcgccg ccggtaccag cctttcggga caggcggtga
     1681 ccgacggtat cctggtcctc atcggagaag ccttcgccac ctgtgaaatc gggccggacg
     1741 ccgccacggt gcgcgtcggg cccggcatca tcggcggcga ggtcaattac cgtctggcac
     1801 cgcatggccg caagatcggt cccgatccgg cctcgatcag cgcctgcaag atgggtggta
     1861 tcgccgccaa caacgcttcc ggcatgtgct gtggtacggc gcagaacagt taccgcaccc
     1921 tggccggtct gcgcgtcgtg ttcgccgacg gatccgtcct cgacaccgag gatgcggcca
     1981 gcgtcgcccg tttccgcgcc agccatgccg ccctggtcga cgaactcg
//
LOCUS       DAOSIT010000453         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_47994_length_2026_cov_8.650939, whole genome shotgun sequence.
ACCESSION   DAOSIT010000453 DAOSIT010000000
VERSION     DAOSIT010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47994_length_2026_cov_8.650939"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2026
                     /locus_tag="PLL14_09855"
     CDS             <1..>2026
                     /locus_tag="PLL14_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOG04273.1"
                     /translation="FTDLHAWCEVYLPGAGWIGLDPTSGLFAGEGHIPLACSPQPSSA
                     SPITGFTEECECEFEHHMKIERVWEAPRVTKPYDEQQWLAIEALGHQIDAELVSGDVR
                     LTMGGEPTFVSIDDHDGAEWNIDALGPTKRLRAAEVFQRLRAKYAPQGLQHFGQGKWY
                     PGEQLPRWSLNCFWRRDGQPVWKNPALYADESRDYGADEVLAGRFLRQLAEVLAVNPG
                     HVFPACEDAYYYLWREHRLPINVDVSNARLDDPLERERLAKIFHQGLDRVVGYVLPLK
                     RRPQGGWQSGQWFLRAGRCFLVPGDSPIGYRLPLDSQPWVAKADYPYIHTPDPTQTFA
                     PLPAHAEIQAQCAISRSQDAGAEEAAVTGEHFAAQGIERIEGAFLHAAEVPPQDGASA
                     VSVTRTSFSAQARNGVLYIFMPPTETLDDYLELVAAVEATAEILATPVILEGYEPPSD
                     PRLTNFRVTPDPGVIEVNIQPSATWDELVERTTHLYEAAHLSRLCTEKFMLDGRHTGT
                     GGGNHFVFGGPTTLDSPFLRRPDLLRSLISYWHNHPSLSYLFSGLFIGPTSQSPRVDE
                     ARNDSVYELEIAFSQFPQPGADAAACVAPWIIDRVLRNLLIDSSGNTHRAEFCIDKLY
                     SPDGPTGRLGLLEMRAFEMPPHARMSLAQQLLLRALIARFWRTPYAPERLL"
BASE COUNT          351 a          704 c          616 g          355 t
ORIGIN      
        1 acttcaccga cctgcacgcc tggtgcgagg tgtacctgcc gggcgccggc tggatcggtc
       61 tcgacccgac ctcggggctg ttcgccggcg aaggacacat cccgctggcg tgctcgccgc
      121 aaccgtcgtc ggcatcgccg atcaccggtt tcaccgagga atgcgagtgc gagttcgagc
      181 accacatgaa aatcgagcgc gtatgggaag cgccgcgggt gaccaagccg tatgacgaac
      241 agcagtggct ggcaatcgag gcgctcggcc accagatcga cgccgagctg gtcagtggtg
      301 acgttcgcct gaccatgggc ggcgaaccca ccttcgtttc gatcgacgac cacgatggcg
      361 ccgaatggaa tatcgacgcc ctcggaccaa ccaagcgcct gcgcgccgct gaagtctttc
      421 agcggctgcg cgcaaaatac gctccgcagg ggctgcagca cttcggtcag ggcaaatggt
      481 atcccggcga gcaattgccg cgctggtcgc tgaactgttt ctggcggcgc gacggccagc
      541 cggtatggaa gaatcccgcg ctgtatgccg acgaaagccg cgactacgga gccgacgagg
      601 tcctcgccgg ccgcttcctg cggcagctcg ccgaggtgct ggcggtcaac ccggggcacg
      661 tcttcccggc ctgcgaggat gcctattatt acctgtggcg cgagcaccgc ctgccgatca
      721 acgtcgacgt gtcgaatgcc cgtctggacg acccgctgga acgtgaacgg ctggcgaaaa
      781 tcttccacca gggactcgac cgggtagtcg gttacgtgct gccgctgaag cgccggccgc
      841 agggtggctg gcaaagcgga cagtggttcc tgcgcgccgg ccgctgcttc ctggtcccgg
      901 gcgattcgcc gatcggttat cgcttgccgc tcgactcaca gccctgggtg gcgaaggccg
      961 actatcccta catccacacc cctgatccga cgcaaacctt cgcgccgctg cccgcccacg
     1021 ccgagatcca ggcgcagtgt gcgatcagcc gcagccagga tgccggtgca gaggaagccg
     1081 cggtcaccgg cgaacacttc gccgcgcagg gcatcgagcg catcgaggga gcgttcctgc
     1141 atgcagccga agtgccgcca caggacggcg cgtcggcggt atcggtcacc cgcacgtcgt
     1201 tttctgcaca ggcgcgcaat ggtgtcttgt acattttcat gccgccgacc gagacgctcg
     1261 atgactacct cgaactcgtg gccgccgtcg aagccaccgc cgagattctc gccacgccgg
     1321 tgattctcga gggttacgag ccaccgtccg acccgcgcct gaccaacttt cgcgtcacgc
     1381 ccgaccctgg cgtgatcgaa gtcaacattc agccctccgc cacctgggac gaactcgtcg
     1441 aacgcaccac gcatctttac gaagccgcgc atctgtcccg cctgtgtacc gaaaagttca
     1501 tgcttgatgg tcgccatacc ggtactggcg gcggcaacca cttcgtcttc gggggaccca
     1561 cgacactcga ttcacctttc ctgcgacgac ccgacctgtt gcgcagcctg atcagttact
     1621 ggcacaacca tccttcgctc tcgtatctct tttcgggcct gttcatcggc ccgaccagcc
     1681 agtcgccgcg cgtcgatgaa gcgcgcaacg attcggtgta tgaactggag atcgccttca
     1741 gccagttccc gcagccgggc gccgacgccg cggcgtgcgt cgctccgtgg attatcgacc
     1801 gtgtgctgcg caatctgctg atcgactcgt cgggcaacac gcaccgcgcg gagttctgca
     1861 tcgacaaact ctactcgccc gacggcccta ccggccgcct cggactgctc gaaatgcgcg
     1921 ccttcgagat gccaccgcac gcgcgcatgt cgctggcgca acagttgctg ctgcgggcgc
     1981 tgatcgcccg cttctggcgc acaccctacg caccggaacg gctgct
//
LOCUS       DAOSIT010000454         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_48026_length_2025_cov_7.637056, whole genome shotgun sequence.
ACCESSION   DAOSIT010000454 DAOSIT010000000
VERSION     DAOSIT010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48026_length_2025_cov_7.637056"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1148
                     /locus_tag="PLL14_09860"
     CDS             15..1148
                     /locus_tag="PLL14_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG04274.1"
                     /translation="MQSVGATVIGAGVIGLACARVLARQGIETVVLERHGVCASETSA
                     RNSEVIHAGFHYPTGSLKARCCVVGRRQLYDFCNTHGVSHRRCGKLVVATSAQQEGQL
                     LLLQRLGHDNGVDDLCLLSASAARALEPELACTAALLSPSTGIIDSHALTLALLGDAE
                     RAGAVLALRSPLRRGSIEDRGIVLESGGTQSLRFRSSIVINAAGLWAPEVAAALAGFP
                     PALIPANFHAKGNYFALARRAPFSRLVYPLPEAAGLGVHLTLDLGGQARFGPDVEWLP
                     DPTPGQPIGEFDYRVDPARASAFVTAIRRYWPALPDDALLPAYAGVRPKISGRSAPAG
                     DFVVQGPAEHGIPGLINLFGIESPGLTSCLAIAEHVLAELQGG"
     gene            1141..1584
                     /locus_tag="PLL14_09865"
     CDS             1141..1584
                     /locus_tag="PLL14_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOG04275.1"
                     /translation="MAERLRAVLDTNIVIDMLHFADPPAHALLGAIERGVLGCFTDQH
                     CLSELERVSAYPQFGLASPAQQALLARYRNLATLCDADGAENYSLPRCRDADDQKFLI
                     LAARCRADLLVTRDRQLLRLARQRNPAPPYVILNAEAAGALLISR"
     gene            complement(1637..>2025)
                     /gene="yaaA"
                     /locus_tag="PLL14_09870"
     CDS             complement(1637..>2025)
                     /gene="yaaA"
                     /locus_tag="PLL14_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOG04276.1"
                     /translation="MGTRLSNARGRDLYAFWGDRITEAVNAALRETGARVLINLASEE
                     YFKSVKPKQLAVPVIQPVFEDWSGGRFRVVSFYAKRARGLMARFAIVNHLQDAAGLKD
                     FAADGYAHAAAASDDQVWVFRRGGAV"
BASE COUNT          342 a          724 c          627 g          332 t
ORIGIN      
        1 cgtgtggagg aaagatgcaa agcgtgggag cgacagtgat cggggccggt gtgatcggcc
       61 tggcctgcgc acgtgtgctg gcgcggcaag gcatcgaaac ggtggttctc gaacggcacg
      121 gcgtttgcgc cagcgaaacc agtgcccgca acagtgaggt gatccacgcc gggttccatt
      181 atccgacagg atcgctcaag gcccgctgct gtgtcgtcgg ccgacggcag ctctatgact
      241 tctgcaatac gcatggcgtc agccaccggc gctgcggcaa actggtcgtc gccacctcgg
      301 cccaacagga agggcaactt ctgctgctgc agcggctggg gcacgacaac ggcgtcgacg
      361 atctgtgcct gctgagcgca tccgcggcgc gcgctctcga accggaactg gcgtgtaccg
      421 ccgccctgct ctctccctcg accggcatca tcgacagcca cgcgctgacg ctcgcgctcc
      481 tcggcgacgc cgagcgcgcc ggcgccgtgc tggcactgcg cagcccgttg cgccgcggct
      541 cgatagaaga caggggcatc gtcctcgaaa gcggcggtac gcaaagcctg cgcttcagga
      601 gttcgatcgt gatcaacgct gccggcctct gggcaccgga ggtcgcggcc gcgctggccg
      661 gcttcccgcc ggcgctgata ccggcaaact ttcacgccaa gggcaactac ttcgcgctcg
      721 ccagacgggc accgttctcg cggctggtct atcccttgcc ggaagccgcc ggcctcggag
      781 ttcatctgac gctcgatctc ggcgggcagg cgcgtttcgg ccccgacgtc gagtggctgc
      841 cagacccaac gccaggccaa cccatcggtg aattcgatta ccgcgtcgac ccggcacgcg
      901 ccagcgcctt cgtcaccgcg attcgccgct actggccagc gctgccggac gatgcgctgc
      961 tgcccgcgta cgccggggtg cggccaaaga tcagcggccg ctcggcgccc gccggggact
     1021 ttgtcgtcca gggccctgcc gagcacggca tcccgggctt gatcaacctc ttcggaatcg
     1081 aatcccccgg actgacctcg tgcctggcca tcgccgaaca tgtgctcgcg gaactgcaag
     1141 gtggctgaac gcctgcgcgc ggtcctcgac accaacatcg tgatcgacat gctgcacttc
     1201 gccgacccac cggcgcatgc gctgctcggg gccatcgagc gcggggtcct gggctgcttt
     1261 accgatcaac actgcctgtc cgaactcgaa agggtgagcg cctacccgca gttcggactc
     1321 gccagtccgg cacagcaggc gctgctggca cgctaccgca acctggcgac gctctgcgac
     1381 gccgacgggg cggagaatta ttccctgccc cgctgccgcg acgccgacga ccagaagttc
     1441 ctgatcctcg cggcacgctg ccgtgccgac ctgctggtga cccgcgatcg ccagttgctg
     1501 aggctcgccc gtcagcgcaa cccggcccca ccatacgtca tcctcaacgc cgaggcagcc
     1561 ggtgcgctgc tcatcagccg ctgacggcca tggcttggca agacaccccg gatcctcgac
     1621 gaccacccgt gcggattcaa accgcgccgc cgcgccgaaa cacccagacc tgatcgtcgg
     1681 aggccgctgc ggcatgcgca tagccgtccg ccgcgaaatc cttcagacct gcggcatctt
     1741 gcaaatgatt gacgattgcg aaacgcgcca tcagcccccg tgcgcgtttg gcgtagaaac
     1801 tcaccacccg gaaccggccg ccgctccagt cctcgaaaac cggttgaatc accgggacag
     1861 cgagttgttt cggcttgacc gacttgaagt actcctcgga cgcgagattg atcagcactc
     1921 gggcgcctgt ttcgcggagt gcggcgttga ccgcctcggt aatccgatca ccccagaagg
     1981 catagagatc ccggccacgt gcgttgctca agcgcgtccc cattt
//
LOCUS       DAOSIT010000455         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_48142_length_2021_cov_11.013225, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000455 DAOSIT010000000
VERSION     DAOSIT010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48142_length_2021_cov_11.013225"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PLL14_09875"
     CDS             complement(<1..140)
                     /locus_tag="PLL14_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG04277.1"
                     /translation="MEIEIDGKVAQVPDGSTIMDAAQQLGAYIPHFCYHKKLSIAANC
                     RM"
     gene            complement(152..1453)
                     /gene="nuoF"
                     /locus_tag="PLL14_09880"
     CDS             complement(152..1453)
                     /gene="nuoF"
                     /locus_tag="PLL14_09880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG04278.1"
                     /translation="MLSVGLDGDNAWRLADYVKRGGYTALRRILEEKVPQGDVINEVK
                     KSVLRGRGGAGFPTGLKWSFMPRSFPGDKYVVCNSDEGEPGTFKDRDILRYNPHSVIE
                     GMAIAAYAMGAGRGYNYIHGEVWEIYQRFEEALDEARAAGFIGNDLLGSGFSFELFAH
                     HGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETFASIPYIMN
                     LGGESYLEAGKANNGGTKLFSVSGHVNRPGNYEVPLGTPFATLLEMAGGMRAGRKLKA
                     CIPGGSSAPVVPAATMMDCTMDYDSISKAGSMLGSGAVIVMDETTCMVKALERLSFFY
                     YEESCGQCTPCREGTSWLYKVVHRIEGGRGRSDDLELLASVTTNIMGRTICALGDAAS
                     MPVQSFIKHFRDEFAYHIEHRKCLVPVDVQRAGSGFFTATA"
     gene            complement(1480..1953)
                     /gene="nuoE"
                     /locus_tag="PLL14_09885"
     CDS             complement(1480..1953)
                     /gene="nuoE"
                     /locus_tag="PLL14_09885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG04279.1"
                     /translation="MLTSESLKRIDREIAKYPADQKQSAVMAALAIAQEEQGWLPSEA
                     IESVANYLGMAPIAAYEVASFYNMYDLRPVGKYKVSVCTNLPCMLSGGVDAGEYLKQK
                     LGIGYNETTPDGLITLKESECMGACGDAPVVIINNRRMCSWMQPEQIDKLLAELK"
     gene            complement(1953..2018)
                     /locus_tag="PLL14_09890"
     CDS             complement(1953..2018)
                     /locus_tag="PLL14_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04280.1"
                     /translation="MLADVVTIIGSQDVVFGEIDR"
BASE COUNT          400 a          636 c          560 g          425 t
ORIGIN      
        1 cacatgcggc agttggcggc gatcgaaagc ttcttgtgat agcagaaatg cgggatatag
       61 gcgccgagct gctgcgcagc atccatgatc gtactgccgt cgggcacttg cgccaccttg
      121 ccgtcgattt cgatttccat cgtctcgtac cctaagccgt tgccgtaaag aagccgctgc
      181 cggcgcgctg gacatcaacg ggcacgaggc acttcctgtg ttcgatgtgg tacgcgaatt
      241 cgtcgcgaaa atgcttgatg aagctctgga ccggcatcga ggcggcatcg ccgagcgcac
      301 aaatggtgcg acccatgatg ttcgtggtga cgctcgcgag cagctcgagg tcatcggagc
      361 gcccccgccc gccttcgatt cggtgcacta ccttgtacag ccaactcgtg ccttcgcgac
      421 acggcgtgca ctgtccgcaa gattcttcgt aataaaagaa agacagtcgc tccagagcct
      481 tgaccatgca ggtcgtctcg tccatgacga tgaccgcacc cgacccaagc atggaacccg
      541 ccttgctgat cgaatcgtag tccatggtgc agtccatcat cgtggctgcc ggcaccaccg
      601 gcgcggaaga accacccggt atgcaggcct tcagtttccg gcccgcgcgc atgccaccgg
      661 ccatttcgag cagcgtcgcg aaaggtgtgc ccagcggcac ctcgtagttg ccgggccgat
      721 tgacgtggcc ggataccgaa aaaagcttgg tcccgccatt gttcgccttg ccggcttcga
      781 gatagctctc gccgccgaga ttcatgatgt aggggatgga agcaaaggtc tcggtattgt
      841 tgatcgtcgt cggcttcccg tagaggccga aagaggctgg gaatggcggc ttgaaccgtg
      901 gctgcccctt cttgccctca atcgattcaa gcagcgccgt ttcctcgccg cagatatagg
      961 ccccgtaacc gtgatgtgca aagagctcga agctgaaacc tgaacccagc agatcgttgc
     1021 cgataaatcc cgctgcccgc gcttcgtcca aagcttcctc gaagcgctgg tagatttccc
     1081 atacttcacc gtgaatatag ttgtatccac gcccggctcc catcgcgtag gcagcgatgg
     1141 ccatcccctc gatcaccgag tgcgggttgt agcgcaggat gtcacggtcc ttgaaggttc
     1201 ccggctcgcc ctcatccgaa ttgcacacca cgtacttgtc accgggaaac gagcgcggca
     1261 tgaaactcca cttgagaccg gtcgggaaac cggcaccacc gcggccgcgc aggaccgatt
     1321 tcttgacctc gttgatgaca tcaccctgcg gcaccttctc ttcgagaatg cgtcgcagcg
     1381 ccgtgtagcc accgcgtttg acgtaatccg cgaggcgcca ggcgttgtcg ccatccaggc
     1441 caacgctgag catcgggttg attgcaccga gaatcgccat tacttgagct ccgccagcag
     1501 tttgtcgatt tgctcgggct gcatccaact gcacatgcga cggttattga tgatcaccac
     1561 cggcgcgtca ccgcaggcac ccatgcattc gctctccttg agcgtgatca ggccatccgg
     1621 cgtcgtttcg ttataaccga ttcccagctt ctgcttcaga tattcaccgg catcgacgcc
     1681 tccggagagc atgcacggca gattggtgca caccgacacc ttgtacttgc cgaccggcct
     1741 gaggtcatac atgttgtaga agctggccac ttcataggcg gcaatcggcg ccatgccgag
     1801 atagttggcc acactctcga tggcctcgct gggtagccag ccctgctctt cctgcgcgat
     1861 cgccagagcc gccatcaccg ccgactgttt ctggtcggcc gggtacttgg caatttcgcg
     1921 atcaattctc ttgagggatt ctgaagtcag catcagcggt caatctcgcc aaaaacaacg
     1981 tcctgggaac caatgatcgt caccacgtcg gccagcatgt g
//
LOCUS       DAOSIT010000456         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_48380_length_2013_cov_6.983146, whole genome shotgun sequence.
ACCESSION   DAOSIT010000456 DAOSIT010000000
VERSION     DAOSIT010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48380_length_2013_cov_6.983146"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLL14_09895"
     CDS             <1..155
                     /locus_tag="PLL14_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG04281.1"
                     /translation="IADDKVVLDGNHPLAGMALVFTCTVTDVRPATAEEISHGHTHDD
                     DCDEDD"
     gene            152..>2013
                     /locus_tag="PLL14_09900"
     CDS             152..>2013
                     /locus_tag="PLL14_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOG04282.1"
                     /translation="MNAGVSIVAVPRQLLRGERRPRVVLVGRLRTGKSSLFLAASSAS
                     PQHRNLLRDGDGYDECLVDVGLEQMSLVDLPPIESLHTLGAHDRVLVKYLLWGDRWPP
                     VAAHEAEQPALAFRSPDVLIQVVDATALQRDLELTLELCLLGKPLVIALNRVDEARRK
                     GLYINTRALSERLGVPVVATVAHMGLGVSEVFVAALAAARKKEPPRTPAPSKYIEVSL
                     ESLRVLLTRPEVVEAFRAPWPFLLMQLAENDDYFLQEFAGHFPELLPEVLAARLAAER
                     QLPRPLSEELHADRHHRAATLFEQVTQFGGSADSGRGQRFLDGIFLHPRWGLIGSLAV
                     FALVLLMVFKVSAILDGWTSAPLADWVQQWQPESTAGVVGRAVIDGLIGLIGIVIPYM
                     LPLVLLLVALEEAGIMHRVAFVVDRGFHHIGLHGGVATPFLVGLGCNVPAISMVAATT
                     GGKERVVATLLLAFVPCSARSAIILAMGGKYLGWLGVFAIFALTLVVLTALGGTLARR
                     YTDVAVGLIQEIPPYALPTWHGLVLRTWERTSDILTIVTPLLVAGSVLLALLNHFGAD
                     QVINLLLSPITHWWLGLPVLLGVPILFGVLRKELSLLMVYQALGSMEIDPFLDWV"
BASE COUNT          313 a          601 c          661 g          438 t
ORIGIN      
        1 agatcgccga cgacaaggtg gtgctcgatg gcaaccatcc actggccggg atggcgctgg
       61 tatttacctg taccgtgacc gacgttcgcc cggcgaccgc cgaagaaatc agccatgggc
      121 atacacacga tgatgattgc gatgaggacg attgaacgcc ggcgtctcga tcgtcgccgt
      181 acctcggcaa ctgttgcgcg gagaacggcg gccgcgcgtg gtcctcgtcg ggcgcttgcg
      241 taccggcaag agcagcttgt ttcttgccgc ctcgagcgct tcgccacagc accgtaatct
      301 gctccgcgac ggcgacggct atgacgagtg tctggttgac gtcggactgg agcagatgtc
      361 gctggtcgac ctgccgccca tcgagtcgct gcacacgctg ggtgcgcacg accgggtcct
      421 ggtcaagtat ctgctgtggg gcgaccgctg gccgccagtt gccgcacacg aagccgaaca
      481 gcccgcactg gcctttcgct ccccggatgt cctgatccag gtcgtcgacg cgacggcact
      541 gcagcgcgat ctggaactga cactcgagct gtgcctgttg ggaaaaccgc tggtgatcgc
      601 gctcaaccgg gtggacgaag cgcgtcgcaa gggcctgtat atcaacacgc gggcgctatc
      661 cgagcgcctc ggcgtgccgg tggtggccac cgtcgcgcac atggggctgg gggtttcgga
      721 agtctttgtg gcggcgctcg cggccgcgcg aaagaaagag ccaccgcgaa caccggcgcc
      781 cagcaagtat atcgaggtca gcctcgaatc gctgcgcgtg ctgctcaccc gaccggaggt
      841 ggtggaggca tttcgagcgc cgtggccgtt tctgctcatg cagttggcgg aaaacgacga
      901 ctattttctg caggaatttg caggtcattt tccggaactg ttacccgagg tgcttgccgc
      961 ccgccttgct gccgaacgtc aattgccccg cccgctgtcg gaggaactgc atgccgaccg
     1021 ccatcaccgg gcggcaaccc tcttcgaaca ggtgacccag tttggtggct cggccgactc
     1081 cgggcgtggt cagcgctttc tcgacggcat cttcctgcat ccgcgctggg gcctgatcgg
     1141 tagtctggca gtgtttgcac tggtcttgct gatggtgttc aaggtcagcg ccatacttga
     1201 cgggtggact tcggcaccct tggcggactg ggtgcagcag tggcagccgg agtctactgc
     1261 tggcgtggtt ggacgggcgg tgattgatgg cctgattggt ctgataggaa ttgtcattcc
     1321 ctacatgctg ccgctggtac tgctgctggt cgcgctcgaa gaggccggga tcatgcaccg
     1381 cgttgccttt gtggtagacc ggggctttca ccacattggt ctgcacggtg gcgtcgcgac
     1441 gccgtttctg gttgggctgg gctgcaacgt gccggcgatc tcgatggtcg ccgcgacgac
     1501 cggcggcaag gagcgcgtcg ttgccaccct gttgctggcc tttgttccct gttctgcacg
     1561 ttccgcaatc attctggcga tgggcgggaa atatctcggt tggctggggg ttttcgccat
     1621 ctttgcactg accctggtgg tattgacggc gcttggtggg acgttggcca ggcggtacac
     1681 cgacgtcgct gtcggcctga tccaggaaat tcctccgtat gccttgccga catggcatgg
     1741 acttgtgctc aggacctggg agcggaccag cgatattctg accatcgtca cgcctctcct
     1801 ggtggccggg agtgtgctcc tggcattgct caaccatttc ggcgccgacc aggtgatcaa
     1861 cctcctgctg tcgccaatca cccattggtg gctcggcttg ccggtgttgc tcggcgtgcc
     1921 gatcctgttc ggggtcctgc gcaaggagtt atcgctgctg atggtttacc aggcgctggg
     1981 aagcatggaa atcgaccctt ttctcgactg ggt
//
LOCUS       DAOSIT010000457         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_48612_length_2006_cov_8.013839, whole genome shotgun sequence.
ACCESSION   DAOSIT010000457 DAOSIT010000000
VERSION     DAOSIT010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48612_length_2006_cov_8.013839"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLL14_09905"
     CDS             <1..395
                     /locus_tag="PLL14_09905"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOG04283.1"
                     /translation="QQDHPEDFVIATGVQYSVRQFVEFAAAELGISVRFEGEGVDEVG
                     IVDSVEGDQAKVKPGAVIVKVDPRYFRPTEVETLLGDPGKAREKLGWTPITSLQELVK
                     EMVVADYNSAKRDALVKLAGFKIFDHHE"
     gene            407..1465
                     /locus_tag="PLL14_09910"
     CDS             407..1465
                     /locus_tag="PLL14_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOG04284.1"
                     /translation="MTETRIYVAGHRGMVGSAIVRILQRRGETRLITRSHAELDLTDQ
                     ASVRAFFEAEKPEQVYLAAAKVGGIHANNTYPAEFIYQNLMIEANIVHEAWRVGVQKL
                     LFLGSSCIYPKLAAQPMSEEALLTGTLEPTNEPYAIAKIAGIKLCESYNRQYGTDYRS
                     VMPTNLYGPGDNYDLQNSHVIPAMIRKLHLAKLAADHDWQAIERDEARNGAIPEDLKR
                     QFHATCSRQLAPCLSLWGTGTPMREFLYVDDMADASIHVMNLDRATYQSHTQVMLSHI
                     NVGTGEDITIRELAELVGKVVGYTGRIEFDPSKPDGTPRKLLDVSRLHALGNQASVGL
                     EEGLARAYADFLAREARP"
     gene            complement(1495..>2006)
                     /locus_tag="PLL14_09915"
     CDS             complement(1495..>2006)
                     /locus_tag="PLL14_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3782 domain-containing protein"
                     /protein_id="HOG04285.1"
                     /translation="QKAEQDRKWEEQNRKWVESGERFDRMHEEIMAQARKHDRSIGAL
                     GARWGTQSEKAFRDALAGILEENFGVRVLNVNEYDDEGVVFGRPEQVELDVIIKNGLL
                     LICELKSSIDKAGMYIFERKARFYEQRHQRKADRLIVISPMIDARAQPVAKRLGIETY
                     GDSLDVEIG"
BASE COUNT          416 a          686 c          537 g          367 t
ORIGIN      
        1 tgcagcagga ccatcccgaa gacttcgtca tcgccaccgg cgtgcaatac agcgtgcgcc
       61 agtttgtcga attcgcggct gccgaactgg gtatcagcgt gcgcttcgaa ggcgagggtg
      121 tcgatgaggt cggtatcgtc gactccgtcg agggcgacca ggccaaggtc aaaccgggcg
      181 ccgtcatcgt caaggtggac ccgcgctatt ttcgcccgac cgaagtcgag accttgctcg
      241 gcgaccccgg caaggcccgc gaaaaactcg gctggacgcc catcaccagc ctgcaggaac
      301 tggtcaagga aatggtcgtg gccgactaca actcggccaa acgcgatgcc ctggtgaagc
      361 ttgccggctt caagatcttc gaccaccacg aataagggcc agagcaatga ccgaaacccg
      421 gatctacgtc gccggccatc gcgggatggt cggctcggcc atcgtgcgta tccttcagcg
      481 gcgtggcgaa acccggctga ttacgcgcag ccacgccgaa ctcgacctga ctgaccaggc
      541 ttccgtgcgc gccttcttcg aggcggaaaa gcccgaacag gtctacctgg ccgccgccaa
      601 ggtcggcggc atccacgcca acaataccta tccagcggaa ttcatctacc agaacctgat
      661 gatcgaggcc aacatcgttc atgaggcctg gcgtgtcggc gtgcagaaac tcctgtttct
      721 cggcagcagt tgcatctatc ccaaacttgc cgcgcaaccg atgagtgaag aagcgctgct
      781 caccggcacg ctggaaccca ccaacgaacc gtacgccatc gccaagatcg ccggcatcaa
      841 gctgtgcgaa agctacaacc gccaatacgg caccgactac cgtagcgtca tgcccaccaa
      901 cctctacggg ccgggcgaca actatgacct gcagaacagc cacgtcatcc cggcgatgat
      961 ccgcaagctg caccttgcca aactcgccgc cgaccacgac tggcaggcca tcgaacgtga
     1021 cgaagcccgg aacggcgcca ttcccgagga tctcaagcgc cagttccacg ccacctgcag
     1081 ccggcaactc gcgccttgtc tctcgctctg gggtaccggc accccgatgc gcgaattcct
     1141 ctacgttgac gacatggccg acgccagcat ccatgtcatg aatctcgacc gggcgaccta
     1201 ccagtcgcac acgcaggtca tgctctcgca catcaacgtc ggcaccggcg aagacatcac
     1261 catccgtgaa ttggccgaac tggtgggcaa ggtcgtcggc tacacgggtc gtatcgaatt
     1321 cgaccccagc aaaccggatg gcacgccgcg gaagctgctc gatgtctccc gcctgcatgc
     1381 cctgggcaac caggccagcg tcggtctgga agaaggcttg gccagggcct acgccgactt
     1441 tctcgccagg gaggcccgtc cctgattcag cgcctgggtg cagccccttg aagatcagcc
     1501 gatttcgacg tctagggaat ccccataggt ttcgattccc agacgtttgg ccaccggttg
     1561 cgcccgcgca tcgatcatcg gcgagatcac gatcaagcga tcggccttgc gctggtgccg
     1621 ctgctcatag aacctcgcct tgcgctcaaa gatatacatt ccggccttgt cgatcgacga
     1681 cttcaattcg caaatcagca gcaggccatt cttgatgatc acatccagct ccacctgctc
     1741 gggccgcccg aacacgacgc cctcatcatc gtattcgtta acattcaaaa cccgcacccc
     1801 gaagttctcc tccagaatgc cggccaatgc gtcccggaaa gccttttccg actgagtacc
     1861 ccagcgcgca ccgagagcac cgatgctacg gtcgtgcttg cgggcctgcg ccatgatttc
     1921 ttcgtgcatt cggtcgaatc gctcccccga ttccacccat ttgcggttct gttcctccca
     1981 ttttcggtct tgctcagcct tctgct
//
LOCUS       DAOSIT010000458         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_48676_length_2004_cov_8.320677, whole genome shotgun sequence.
ACCESSION   DAOSIT010000458 DAOSIT010000000
VERSION     DAOSIT010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48676_length_2004_cov_8.320677"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..932
                     /locus_tag="PLL14_09920"
     CDS             12..932
                     /locus_tag="PLL14_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG04286.1"
                     /translation="MARRHTLTYLAGIAVAGLALSACGSSSLTPTATTAASKAAGAAT
                     SAASAADPALVAKIPAKIKTAGKIVVGTDATYAPNEYLDTDGKTVIGMDIDIFDAVAA
                     KFGLKVEYVPASFDSIILGVGSGKYDVGVSSFTINDKRKAEANMISYFSAGTQWVTQK
                     GNPKKVDPNNACGLNIGVQKGTVQADEDLPAKSKACTDAGKPAINAIVDGDQGKVTTS
                     VLSGKADAMLADSPIGLYAVKQTNGQLEALGQIYDAAPYGFVVAKDQKDFADAIASAL
                     KAIGTSGEYKAALAKWGNESGGITTFAVNP"
     gene            932..1876
                     /locus_tag="PLL14_09925"
     CDS             932..1876
                     /locus_tag="PLL14_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG04287.1"
                     /translation="MSDQTTDRPGVIHARPVRHPWRWVAIVILAVLFAMMVSSFVTNP
                     RWDWAYAFQIMQQTPVIEGLWIGTIFGTIASMILGVGLGVVLAVMRLSDNPVLRAVAF
                     AYTWFFRAMPRYVLLAILGLGIGFLYSEIRFGVPFGQQISSALGLSGDLTFFGFSWNS
                     IASTIWVGIIGLGLSEAAYMAEIARAGILSVDKGQNEAAQALGMSSGQTMRRIVLPQA
                     MRVIVPPTGNETIAMVKDTSLLAAVPVLTELYYQAYLFGQRSFKIMPAFVAATLWYLI
                     VCSILMVGQSYLERYFGRGFGAVPKKTKQKLMKIEADH"
     gene            1876..>2004
                     /locus_tag="PLL14_09930"
     CDS             1876..>2004
                     /locus_tag="PLL14_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="HOG04288.1"
                     /translation="MATARTSQPGEMPLVRAVNVLKSFHGNQVLKGIDMDVHRGEVV"
BASE COUNT          367 a          677 c          610 g          350 t
ORIGIN      
        1 aggaaacatc catggcacgt cgtcacaccc tgacctacct ggctggcatc gcagtcgcgg
       61 gactcgcgct gtcggcttgt ggctccagct cactgacccc caccgcaacg accgccgcga
      121 gcaaggccgc cggcgcagca acgtccgccg cgagcgcggc tgacccggcc ctcgtcgcga
      181 agatcccggc caagatcaag acggccggca agatcgtcgt cggcacggac gcgacgtacg
      241 cgcctaacga atacctcgac accgatggca agaccgtcat cggcatggac attgacatct
      301 tcgacgctgt ggccgccaag ttcggcctca aggtcgagta cgtcccggcc agcttcgact
      361 cgatcatcct cggtgtaggc agcggcaagt acgacgtcgg cgtctcgtcg ttcacgatca
      421 acgacaagcg caaggccgaa gccaacatga tcagctactt cagcgccggc actcagtggg
      481 tcacccagaa gggcaatccg aagaaggtcg accccaacaa cgcctgcggt ctgaacatcg
      541 gcgtccagaa gggcacggtc caggccgacg aggacctgcc cgccaagagc aaggcctgca
      601 ccgatgcagg taagccggcc atcaacgcga tcgtcgacgg cgatcagggc aaggtcacga
      661 cctcggtgct gtccggcaag gctgacgcca tgctcgccga ctcgcccatc ggcctctacg
      721 ccgtcaagca gaccaacggc cagctcgagg cgctcggcca gatctatgac gccgccccct
      781 acggcttcgt cgtcgccaag gaccagaagg acttcgctga cgcgatcgcc tctgcgctga
      841 aggcaatcgg aaccagtggt gagtacaagg cggccctggc caagtggggc aacgagtccg
      901 gcggcatcac caccttcgcg gtcaacccct gatgtctgac cagaccaccg accggccggg
      961 cgtgattcac gcccggccgg tccggcatcc gtggcgctgg gtggcgatcg tgatcctcgc
     1021 cgtcctgttc gcgatgatgg tgagttcatt tgtcaccaat ccccgctggg actgggcgta
     1081 cgccttccag atcatgcagc agaccccggt gatcgagggc ctgtggattg gcaccatttt
     1141 cggcaccatc gcgtcaatga tcctcggtgt ggggctcggg gtcgtcctgg cggtgatgcg
     1201 cctctcggac aacccggtgc tgcgcgcggt ggccttcgcc tatacgtggt tctttcgggc
     1261 gatgccgcgc tatgtcttgc tggccatcct tgggctgggc atcggcttcc tctatagcga
     1321 gatccggttc ggcgtcccct tcgggcagca gatctcatct gccctgggtc tgtcgggcga
     1381 cctaacgttc ttcggcttct catggaactc catcgccagc accatctggg tcgggatcat
     1441 cggtctgggc ctgtccgaag cggcctacat ggccgagatc gcccgcgcgg gcatcctgtc
     1501 ggtcgacaag ggccagaacg aggcggccca agccctcggt atgagcagcg gacagaccat
     1561 gcgccgcatc gtgctgccgc aggccatgcg ggtgattgtc ccccccacgg ggaacgagac
     1621 cattgcgatg gtcaaggaca cgtcgcttct cgcagcggtt ccggtcctca ctgagctcta
     1681 ctaccaggcc tatttgtttg ggcagcggag cttcaagatc atgcccgcct tcgtcgcggc
     1741 caccctgtgg tacctcatcg tctgttcgat cctcatggtc ggccagtcct acctggagcg
     1801 ctacttcggc cgcggcttcg gtgcggtacc caagaagacg aagcagaaac tcatgaagat
     1861 cgaggcggac cactgatggc gacggcacgc acctcacaac caggcgagat gcccctggtc
     1921 cgcgccgtca acgtgctcaa gtccttccac ggcaaccagg tgctcaaggg catcgacatg
     1981 gacgtgcacc gcggcgaggt cgtc
//
LOCUS       DAOSIT010000459         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49003_length_1994_cov_10.083032, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000459 DAOSIT010000000
VERSION     DAOSIT010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49003_length_1994_cov_10.083032"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLL14_09935"
     CDS             complement(<1..634)
                     /locus_tag="PLL14_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04289.1"
                     /translation="MSRSRQRASHRKRPAHNPPQRMAIPVQEIEAIVEQSGARPLTEA
                     EQATLKAAVETLARITAELETKETTLARLRRILFGAPTESTANVLGEAEKGDTPPADT
                     PTRPSAAGDDEKAAGQATSDATSPPGVAEPKKKRPGHGRNGAADYPRAPHIAVPHPTL
                     RHGDPCPVDGCTGRVYGQRGEPAVLVRITGVAPLLAEVYELERLRCGLCGT"
     gene            complement(960..1307)
                     /gene="tnpB"
                     /locus_tag="PLL14_09940"
     CDS             complement(960..1307)
                     /gene="tnpB"
                     /locus_tag="PLL14_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852976.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOG04290.1"
                     /translation="MLALNARLRVWVAAEPADFRCGIDGLARRVRQKLAQDPFSGGVF
                     VFCNRRRTAVKLLVYDGQGFWLCQKRLSQGHYRFWPQASPGKLLAPHALAVLLSGGDY
                     AAAGGIPEWRAVA"
     gene            complement(1295..1711)
                     /locus_tag="PLL14_09945"
     CDS             complement(1295..1711)
                     /locus_tag="PLL14_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04291.1"
                     /translation="MSPSLPATAPQLAEIAAALAQWRSTRRRSSRIPEDLWSQATELA
                     ERYGIAKVAVALKLDYKSLKHRLTARNAAEDARGAPVSSSFVELDLGLPLAPPVCVLV
                     LTDRAGRALRIELPPAASSHIAEVARSLWQVQPCSR"
     gene            complement(1772..>1994)
                     /locus_tag="PLL14_09950"
     CDS             complement(1772..>1994)
                     /locus_tag="PLL14_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04292.1"
                     /translation="GRGSRFSVRLPGIIAGAGAPTASVPSPASTAATVAGRGTLILVV
                     EDDGRLVPIVTRLIETLGYAVLCPFGNAA"
BASE COUNT          304 a          671 c          670 g          349 t
ORIGIN      
        1 cggtgccaca caagccgcaa cgcagccgtt cgagttcgta gacctccgcg agcagcggcg
       61 cgacgccagt gatacgaacc aggaccgctg gctctcctcg ttgcccatac acccgaccgg
      121 tacacccgtc gacgggacac ggatcgccgt gccgtaacgt cggatgcggt acggcaatgt
      181 gcggcgctcg cggataatcg gccgccccgt tcctgccgtg tcctgggcgc ttcttcttgg
      241 gctctgcgac cccaggtggg gatgtggcgt ccgaagtcgc ctggccggcg gctttctcgt
      301 cgtcgcctgc cgccgatgga cgggtcggcg tgtccgctgg cggcgtgtcg cccttctccg
      361 cttccccgag cacattggcc gtgctctcgg tcggcgcgcc gaagagaatc cgccgtagcc
      421 gtgccagcgt ggtttccttc gtctcgagtt cggcagtgat ccgcgcgagg gtctccaccg
      481 ctgccttcag cgtcgcctgt tcggcctcgg tcagcggtct ggctccgctc tgctcgacaa
      541 tggcctcgat ctcctgcacc gggatggcca tccgctgcgg cgggttgtgt gcaggccgct
      601 tccggtggga tgcccgctgc cggctccggc tcatggccgt acgccaacga gctggccgaa
      661 gtccccccgc aaccgcttcc gggaacgctt cgagcgatgg cggggttcct tgttgccact
      721 gaccgcgcgg atgctcgcaa cctcctcggc gcggaactcc cgtctgccgt aagcccagct
      781 tggcaaactc gcaagcggca ttctcagtcc tccagcagtg accctttcta cccaaggtat
      841 tctgccaaag accagcgacg atgtctagtc ctttcgcgaa aggtcgtttt cggtatcccc
      901 gttgcggtgg gtgacgaccg gctttcggaa gttctgcttg cgggctgcgc tcgcgaaggt
      961 caggcgaccg ctcgccactc cgggattccg ccggccgcgg cgtagtcgcc tcccgagaga
     1021 aggaccgcca aggcgtgcgg cgcgaggagc ttccccggtg acgcctgcgg ccagaagcgg
     1081 tagtgcccct gcgacagccg cttttgacag agccagaagc cttgcccgtc atagaccagg
     1141 agtttgaccg ccgtgcgccg gcgattgcag aaaacgaaca cgccgccgct gaacgggtct
     1201 tgggccagtt tctgccgcac acgtcgggcg agaccgtcga tgccgcaacg aaaatccgcg
     1261 ggctcggcag ctacccagac acgcaggcgc gcgttcagcg cgagcatggt tggacctgcc
     1321 agaggctacg cgcgacctcg gcgatgtggc tgctggccgc tggtggcagt tcgatgcgca
     1381 gcgcgcggcc cgctcggtcg gtgagaacca ggacgcaaac gggtggcgcc agcggcaggc
     1441 cgagatcgag ttcgacgaag ctcgatgaca cgggcgcgcc gcgcgcgtct tcggcggcgt
     1501 tcctggcggt caggcggtgc ttcaggctct tgtagtcgag cttcagggcg acggcaacct
     1561 tggcgatgcc gtaccgctcg gcgagttcgg tcgcttgcga ccacagatcc tccggaatgc
     1621 gagaactgcg gcggcgggtg ctccgccact gggcaagcgc cgcggcgatt tcggccagtt
     1681 gcggggcggt tgcaggtaaa gacggtgaca ttggtaggcc tctcgaacgg tcgttacggg
     1741 cctaccaagg taccaggcca gcgcagggat ttcacgcagc attgccgaaa ggacacagta
     1801 cggcgtaacc cagggtttcg atcaggcgcg tgacgatcgg caccagccgg ccatcgtctt
     1861 cgacgaccag aatcagtgtc ccgcgcccgg ccaccgtggc agcggtgctc gctggtgacg
     1921 gcacgctagc ggtgggcgcg cctgcgccag cgataattcc cggcagccgc accgagaagc
     1981 ggctgccgcg gccg
//
LOCUS       DAOSIT010000460         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49004_length_1994_cov_9.301186, whole genome shotgun sequence.
ACCESSION   DAOSIT010000460 DAOSIT010000000
VERSION     DAOSIT010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49004_length_1994_cov_9.301186"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /gene="uvrC"
                     /locus_tag="PLL14_09955"
     CDS             complement(<1..1595)
                     /gene="uvrC"
                     /locus_tag="PLL14_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG04293.1"
                     /translation="MTEPFDAKSLLATLTELPGVYRMLDDAGNVLYVGKAKNLRKRLA
                     SYFRDHHPSPRIALMVAQIAQVETTVTRSEAEALLLENNLIKSLSPRYNILFRDDKSY
                     PYIVLTHDNYPRLGFFRGSTDKRADYFGPFPSSPAVRASIHLLQKMFRLRTCENPVFN
                     NRTRPCLLYQIKRCSAPCVGLIDPVRYAQDLQMATLFLQGRQQDVIGSLSAAMDQAAA
                     KLAFEQAAVYRDQIQSLRQVQEKQYVESGKDGDVDIVAAVYDGGVSCVNLAMVRGGRH
                     LGDRPQFPAHAADSTALEVLTAFLYQHYLAHPIPGRILVNLALSDGEVTDWLSATAAQ
                     TVRVGEARTTVQRIWVEMAEQNARLAITARRSVLSRQEARLEALCTTLGLDLEDGKEA
                     RIECFDVSHTQGELTVAACVVYQGNGMRKSEYRRFNIQNIQPGDDYAAIQQAVQRRYE
                     KLPSGEGLAPTLILIDGGSGQVGIAAAALDELGLAELPLVGVAKGEARKPGLETLIFA
                     DGREPLQLPPEHPALHLIQEVRD"
     gene            1640..>1994
                     /gene="earP"
                     /locus_tag="PLL14_09960"
     CDS             1640..>1994
                     /gene="earP"
                     /locus_tag="PLL14_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOG04294.1"
                     /translation="MLSRSPVPSVTTIAARPDWDVFCRVIDNFGDIGVCWRLARQLAG
                     EHALHVRLWVDDFDSFRLLYPELDPGLSIQVRQGVEIRRWENCFTPVVPARVVVEGFA
                     CHLPESFVEHMAHLSP"
BASE COUNT          375 a          592 c          608 g          419 t
ORIGIN      
        1 tcgtcacgga cttcctgaat caggtgcagt gccggatgtt caggaggcaa ttgtaacggc
       61 tcgcggccgt ccgcgaaaat caaggtttcc aggcccggct tgcgtgcttc gcccttggcg
      121 acaccgacga gcggcagttc agcgagacca agttcatcga gcgccgcagc cgcaataccg
      181 acctggccgc tcccgccgtc gatcaggatc agtgtcggcg ccaagccctc gccgctgggc
      241 agtttttcgt agcggcgctg gaccgcctgc tgtatcgcag cgtagtcgtc accaggctgg
      301 atgttctgga tgttgaagcg ccggtattca gacttgcgca tgccgttacc ctgatagacc
      361 acacaggcgg cgacggtcaa ctcgccttgc gtgtggctga cgtcgaaaca ttcgatgcgt
      421 gcctccttgc catcttcgag atcaagaccc aaggtcgtgc agagcgcttc gagtcgcgcc
      481 tcctgacgcg acaacacact gcgtcgggcg gttatcgcca ggcgcgcatt ctgttcggcc
      541 atctccaccc agatgcgctg cacggtagtc cttgcctcgc caaccctgac tgtctgtgcg
      601 gccgttgcgc tgagccagtc ggtgacctca ccatcactca gagccaggtt gacaagaatg
      661 cgccccggaa tcgggtgtgc aagatagtgc tggtagagga acgcggtaag tacttcaagt
      721 gccgtcgaat cggcggcatg ggcaggaaac tgcggcctgt cgccgagatg tcggccaccg
      781 cgaaccatgg ccagattcac acaggacacg ccgccgtcgt aaactgcggc gacgatatcc
      841 acgtctccgt ccttgccact ctcgacgtac tgcttttcct gtacctgtcg caaggactgg
      901 atttggtctc gatagactgc cgcctgttca aaggcgagtt tcgctgccgc ctgatccatt
      961 gccgccgaca gactgccgat cacgtcctgt tgtcgaccct gcaggaacag cgtcgccatc
     1021 tgcaagtcct gggcataacg cacagggtcg atcagaccga cgcagggtgc ggagcagcgt
     1081 ttgatctggt agagcagaca cggccgcgtg cggttgttga acaccggatt ctcgcaagtg
     1141 cgcagacgaa acatcttctg tagcagatgg atactcgcgc gcaccgccgg tgacgaggga
     1201 aaagggccga agtagtcggc gcgcttgtcc gtggagccgc gaaagaagcc cagccgcggg
     1261 taattgtcgt gcgtcaggac gatgtaaggg taggacttgt catcgcgaaa caggatgttg
     1321 taacgtggtg acagactctt gatcagattg ttttcgagca gcagtgcctc ggcctcggag
     1381 cgcgtcactg ttgtttcgac ttgggcgatc tgcgcaacca tcagcgcgat tcgcgggctg
     1441 ggatgatggt cacgaaaata agaggccagc cgctttctga ggttctttgc cttcccgacg
     1501 tagaggacat tgcctgcgtc atcgagcatc cgataaacgc ctggcaattc ggtcagggtg
     1561 gcaagcagcg atttggcgtc gaaaggttcg gtcatgatca ttcgcgtcaa cccaaggctg
     1621 acgtaggata gcgacttcga tgttatcacg ctcgcccgtg ccgtccgtaa ccaccattgc
     1681 cgcccgtccc gactgggatg tgttctgcag ggtcatcgac aacttcggcg atatcggcgt
     1741 ctgctggcgc ctggcgcgac aactggcggg tgaacacgca ttgcatgtgc ggttgtgggt
     1801 cgatgacttc gacagctttc ggctgctgta cccggaattg gacccagggc tgtcgatcca
     1861 ggttcgtcag ggggtcgaga ttcgccgctg ggagaactgt ttcacccccg tcgtccccgc
     1921 gcgggtcgtc gtggaaggct tcgcctgcca tcttccggaa tccttcgtcg aacacatggc
     1981 gcatctgtcg ccgc
//
LOCUS       DAOSIT010000461         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49046_length_1993_cov_7.007740, whole genome shotgun sequence.
ACCESSION   DAOSIT010000461 DAOSIT010000000
VERSION     DAOSIT010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49046_length_1993_cov_7.007740"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLL14_09965"
     CDS             complement(<1..449)
                     /locus_tag="PLL14_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG04295.1"
                     /translation="MDVTMARSGRREHGLILVLNCGSSSIKFAVFDSSAAPLPRQALW
                     NGKVQGIGGANPDFGETGVAPFSIELDTAHPYRAALRLIRDRVSRRLDGRHVGTVAHR
                     IVHGGSKYSMPVRLDANVLADLKGYIPLAPLHQPFALEAVEILLREQP"
     gene            complement(529..1281)
                     /locus_tag="PLL14_09970"
     CDS             complement(529..1281)
                     /locus_tag="PLL14_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG04296.1"
                     /translation="MQYSFSGRVALVTGAGSGIGEAIARLLASNGLNVVVSDVSADNA
                     QRVTSLINAEGGHAVANVADVACIDEVQAAVACAVDTFGDLHFAVNNAGIGGDQSPAG
                     ELDPAAWRRVIDVNLNGVFYGLRHQIPAILRSGGGAIVNVSSILGVVGDAANPAYVAA
                     KHGVTGLTRSAGLAYASKGIRINSIHPGYVRTPILDFLDESALQKAVDLHPIGRLGTS
                     DEIAHAVTFLLSEGSSFLVGTQLIADGGYTAR"
     gene            complement(1343..>1993)
                     /locus_tag="PLL14_09975"
     CDS             complement(1343..>1993)
                     /locus_tag="PLL14_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOG04297.1"
                     /translation="EVEALMKGSLHTDELMSALVSAAAGLRTKRRVSHCFLLQTPAYP
                     RPFIVTDAAINIAPNLEQKADIIRNAIELAQVIGVREPKVAILAAVETVSPTMAATLD
                     AAALCKMADRGQITGGLLDGPLAFDNAVSIAAARIKGIVSEVAGQADILVVPDLESGN
                     MLAKQLIFMGGAASAGIVLGAKVPVILTSRADSRDTRIASCAIALMLAHHYRLSPP"
BASE COUNT          367 a          612 c          659 g          355 t
ORIGIN      
        1 ggctgctccc gcagcaggat ctccacggct tcgagcgcga atggctggtg cagcggggcc
       61 agaggaatgt agcccttgag gtcggccagg acatttgcgt ccaggcgcac gggcatggag
      121 tatttgctgc cgccgtggac gatacggtgg gcgaccgtac ctacgtggcg gccgtcgagc
      181 cgccggctga cacgatcgcg gatgaggcgc agtgcggcgc gatacggatg tgccgtgtcc
      241 agttcgatcg agaacggcgc aactccggtc tctccgaaat ccgggttggc gccgccgatt
      301 ccctgcacct tgccattcca caatgcctga cgcggcagtg gcgcggccga ggaatcgaac
      361 accgcaaact tgatgctgga tgaaccgcag ttcagcacaa ggatcagtcc gtgttcgcgg
      421 cgcccggagc gggccatggt gacgtccatc aggtcgtcct ctctgcgcgc cgtccgcgcg
      481 acttcgccga ggtgtgcggg ggtgtctgct gtctcatcgt cttcagactc agcgcgccgt
      541 gtagccgcca tcggcgatga gctgggtgcc cacaaggaag ctgcttcctt ccgacagcaa
      601 aaacgtcacg gcatgggcga tttcgtccga tgtacccaga cgaccgatcg gatgcaggtc
      661 aacggccttc tggagggccg attcatccaa gaaatccagg atgggcgtac gcacgtaacc
      721 gggatgaatc gaattgatcc ggattccctt ggacgcatag gccagtcctg ccgagcgggt
      781 cagcccggtc acgccatgtt tggctgcgac gtacgcaggg ttcgctgcgt cgcccaccac
      841 ccccaggatg gaggagacgt tgacgatggc gccgcctccc gagcgcagga tggcgggaat
      901 ctgatggcgc aggccataga acacgccgtt caggttgaca tcgatcaccc ggcgccaggc
      961 ggcagggtcc agttctcccg ccgggctctg gtcgccaccg ataccggcgt tgttgacggc
     1021 aaaatgaagg tcgccgaacg tatcgaccgc gcaggccacg gcagcctgga cctcatcgat
     1081 gcatgccacg tcggccacgt tggccacggc gtggccgccc tcggcgttga tgaggctggt
     1141 gacgcgctgc gcattgtcgg cgctgacatc ggagacgacg acattcaggc cgttgcttgc
     1201 aagcagccgg gcaatggcct cgccaatgcc agaccctgca ccggtgacca gggctacgcg
     1261 gccagagaat gagtactgca tggtgtttcc tccaaagggg tggattaaaa agagggggtt
     1321 aataagttgc cgataagtcg gctcaagggg gtgacagccg atagtggtgg gccagcatca
     1381 gtgcgatcgc gcatgaggcg atgcgtgtat cacgcgagtc ggcgcggctg gtcagaatga
     1441 ctggcacctt ggctccaaga acgatcccgg cgctggctgc gccgcccatg aagatcaact
     1501 gcttggcaag catgttgccg ctctccaggt caggcacgac gaggatgtca gcttgcccgg
     1561 cgacctcgga aacgatcccc ttgatacgcg cagcggcgat ggagacggcg ttgtcgaagg
     1621 ccagcggccc gtcgagcagg ccgccagtga tctggccgcg atcggccatc ttgcacagcg
     1681 ccgcagcgtc cagcgtggcc gccatcgtcg ggctcaccgt ctcgaccgcg gccaggatcg
     1741 cgaccttggg ttcgcgcacg ccgatcacct gtgccagctc gatggcgttg cggatgatgt
     1801 ctgccttctg ttccagattc ggggcgatat tgatcgccgc atcggtgacg atgaacgggc
     1861 gcggataggc aggtgtctgc aacaaaaagc aatggctgac ccgacgtttg gtgcgcaacc
     1921 ccgccgcagc cgaaaccagc gcggacatca gctcgtcggt gtgcaggctc cctttcatca
     1981 gggcttcgac ttc
//
LOCUS       DAOSIT010000462         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49154_length_1990_cov_8.635142, whole genome shotgun sequence.
ACCESSION   DAOSIT010000462 DAOSIT010000000
VERSION     DAOSIT010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49154_length_1990_cov_8.635142"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..695
                     /locus_tag="PLL14_09980"
     CDS             33..695
                     /locus_tag="PLL14_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG04298.1"
                     /translation="MRVLLVEDDPMIGKSVQQGLRQDGHTVDWVRESRAAELALATTS
                     YEMLLLDLGLPGKSGLELLAQLRRAAHRIPVLVITARDSVADRILGLDSGADDYLVKP
                     FDLDELGARMRAVLRRHAGRADPVIALGNLRMNPATHELTQNGQPVVLSAREFALLQA
                     LLEQPGVPLSRARLEERLYGWGEEVESNAIEVYIHSLRRKLGAECIRNIRGVGYLVPA
                     SQ"
     gene            692..>1990
                     /locus_tag="PLL14_09985"
     CDS             692..>1990
                     /locus_tag="PLL14_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04299.1"
                     /translation="MKSIRHQLLVALLSAISLTTLLGALATYRTARAEANAMFDYHLR
                     QLALSLRNQVFQNGPGRDFRMEEDAQDFSVQIWSPDGTRIYLSHPRQELPRQTPIGFA
                     TIDSGHGQWRVFGMQQGGQTIQVAQPMLLRDQLALAAALRTVAPFLLMLPMLGVLIWV
                     VVGRGLRPLDAVARAVSTRTPVALDPLPHERVPSEVLPLLLALNDLLARLKRALDAQR
                     EFIADAAHELRTPLAALSLQVQLAERAADSASRAEAFAEVKGGLKRATHAVQQLLTLA
                     RQEPGGGERALAAVKLADLVAGVIAEHSPLAEARRIDLGATPTTGEPVVDGEEEALRI
                     LVGNLVSNAVRYTPPGGKVDVAVADGAEGPRIEVCDSGPGIPVDERERVFDRFYRRAQ
                     NDEAGSGLGLAIVRRIAERHRASVVLDTAPLGGLRARVQFK"
BASE COUNT          307 a          694 c          688 g          301 t
ORIGIN      
        1 cgcctgttct ctcctccggt cagccagcca cgatgcgcgt gttactcgtc gaagacgatc
       61 cgatgatcgg caagagcgtt cagcagggac tgcgccagga tggccacacg gtggactggg
      121 tgcgcgagag ccgggccgcc gaactggctc tggccaccac cagctacgag atgctgctgc
      181 tcgacctcgg cctccccgga aaatccggcc tcgaactgct cgcccagctc cggcgggcag
      241 cccatcgcat cccggtactg gtgatcaccg cccgtgattc ggtcgccgac cggatcctcg
      301 gactcgacag tggtgccgac gactacctgg tcaagccatt cgacctcgac gaactcggcg
      361 cgcgcatgcg cgcggtgctg cgtcgtcacg ccgggcgggc cgatccggtc atcgcgctgg
      421 gcaatttgcg gatgaatccg gcgacccatg agctgacgca gaatggtcaa ccggtcgtgc
      481 tctcggcgcg tgagttcgcc ctgctgcagg cgctgctcga gcagccgggc gtgccgctgt
      541 cgcgggcaag actcgaggag cgcttgtacg gctggggcga ggaagtcgaa agcaacgcga
      601 tcgaagtcta catccactcg ctgcgccgca agctcggcgc cgagtgcatc cggaacatcc
      661 gcggtgtggg ctacctggtg cccgccagcc aatgaagtcg atccgccacc agctcctggt
      721 cgccctgttg tcggcgatca gcctgaccac cctgctcggc gcactggcga cctaccggac
      781 ggcgcgcgcg gaggccaacg cgatgttcga ttaccacctt cggcaactgg ccttgtcgct
      841 gcgcaatcag gtcttccaga acggaccggg acgtgacttc cggatggaag aagacgcaca
      901 ggacttctcg gtccagatct ggagcccgga cgggacccgc atctaccttt cgcacccccg
      961 ccaggaactt ccccggcaga caccgatcgg ctttgcgacg atcgatagcg gccatggcca
     1021 gtggcgggtt ttcgggatgc aacagggcgg gcagacgatc caggtggcgc agccgatgct
     1081 cctgcgtgac cagttggcgc tcgctgccgc gttgcggacg gtggcgccgt tcctgctgat
     1141 gctgccgatg ctcggggtgc tgatctgggt cgtggtcgga cgtggactgc gaccgctcga
     1201 tgccgtggcg cgggcggtga gcacgcgcac gccggttgcg ctcgacccct tgccgcacga
     1261 gcgtgtcccc agcgaggtgc tgccgctgct gctggcgctg aacgatctgc tggcccggct
     1321 caagcgcgcg ctcgacgcgc agcgggaatt catcgccgac gccgcccacg agctgcgcac
     1381 gccgctggcg gcgctgagcc tgcaggtgca gctcgccgaa cgcgccgctg acagcgcgtc
     1441 acgtgccgag gccttcgccg aagtcaaggg cggactgaag cgcgccacgc acgcggtgca
     1501 gcaactcctg acgctggccc gccaggagcc cggcggcggc gagcgcgcgc tggccgcggt
     1561 gaagcttgcg gacctcgtcg ccggcgtcat cgccgagcat tcgccgctgg ccgaagcgcg
     1621 gcggatcgac ctcggggcga cgccgacgac cggcgagccg gtggtcgacg gcgaggaaga
     1681 ggcgctgcgc attctggtcg gcaacctggt cagcaacgcg gtccgctaca ccccgcccgg
     1741 cggcaaggtc gacgtcgcgg tcgccgacgg cgccgaggga ccccgcatcg aagtctgcga
     1801 cagcggcccc ggcattcccg tcgacgagcg cgaacgcgtc ttcgaccgct tctatcgccg
     1861 cgcgcagaac gacgaagccg gcagcggcct ggggctggcc atcgtccggc gcatcgccga
     1921 gcgccatcgg gcaagcgtcg tgctggacac ggcgccgctg ggcggcctgc gcgcgcgcgt
     1981 gcaattcaag
//
LOCUS       DAOSIT010000463         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49185_length_1989_cov_8.528956, whole genome shotgun sequence.
ACCESSION   DAOSIT010000463 DAOSIT010000000
VERSION     DAOSIT010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49185_length_1989_cov_8.528956"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLL14_09990"
     CDS             <1..639
                     /locus_tag="PLL14_09990"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOG04300.1"
                     /translation="ASRWPARGMIRYRVDRGDQGFEIGFSIHRWEVIDGAYRLTAVTE
                     TNGLVALFRPLRFEVESRGRLTAAGLLPEHVVTRQEGRETGTTADFDWEKMELRVANR
                     PARALLPGTQDVLSFAYQLGLIADLAAHGSLPIATGKAYANYRIEIVGDEEIETPAGT
                     FRCLHLRVPGVATTELWLAYDRSLLPVKVQHIDRKGALFVQLAVTIEVSPEP"
     gene            639..1901
                     /locus_tag="PLL14_09995"
     CDS             639..1901
                     /locus_tag="PLL14_09995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOG04301.1"
                     /translation="MNVRFSPALFRHAEVLLSELLRSSFPADLVVSHYFRQHRELGHA
                     DRGFVAETVFSVLRRKRSLSARCADEVTSRRLLLAALACLHGMNRRELAVVLSESESK
                     WLGQAKAVHLDDLPPAVRLDLPDWLHGELNRQFAAEAVEQLAAALNQPAPLDLRVNAL
                     KAHRQEVLARLLADGLTAAPCPYSPLGIRLAGKPALSRHPLFLDGSIEVQDEGSQLLG
                     FLLQPRRGEMVADFCAGAGGKTLLLGAMMRSQGRLYAFDVDERRLARLKPRLARSGLS
                     NVHPVRIDSERDTKIRRLAGKLDRVLVDAPCSGLGTLRRNPDLKWRQTPASVAELCTK
                     QGAILAAAGDLLKPGGRLVYATCSVLEAENEVIVQNFLARFPEFVLCSAQEILARQEI
                     AIDVGERLRLLPQHHGTDAFYGAVMERK"
BASE COUNT          322 a          641 c          647 g          379 t
ORIGIN      
        1 gcctcacgct ggcccgcacg cggaatgatt cgttatcggg tcgaccgcgg tgaccagggt
       61 ttcgagatcg gcttctccat acaccgctgg gaagtcatcg acggggcata ccggctcact
      121 gccgtcaccg agacgaacgg gctggtcgcg ctgttccggc cgctgcgctt cgaagtcgaa
      181 agtcggggcc ggctgaccgc ggcaggcctg ctccccgaac atgtcgtcac ccgccaggag
      241 ggccgcgaaa ccggcaccac ggccgatttc gactgggaaa aaatggagtt gcgcgtcgcc
      301 aatcgaccag cgcgagcctt gctccctggt actcaggatg tcctgtcgtt tgcctaccaa
      361 ctgggtctga tcgccgacct tgccgcgcac ggcagcctgc cgattgccac cggcaaggcg
      421 tacgccaact atcggatcga gatcgtcggc gacgaggaga tcgagacgcc ggccggcacc
      481 tttcgctgcc tgcacctgcg cgtaccggga gtggccacca ccgaactctg gctggcttac
      541 gaccgctcgc tgctgccggt caaggtccag catatcgacc gcaagggcgc tcttttcgtc
      601 cagcttgcgg tcaccattga agtcagcccg gaaccatgat gaacgtgcgt ttttctcctg
      661 cccttttccg ccatgccgaa gtgctgctct ccgaattgct gcgctcgtcc ttcccggccg
      721 acctggtcgt ttcgcactat tttcgacagc atcgcgaact cggccatgcc gatcgcggct
      781 tcgttgccga aacggtgttc tcggtgctgc gtcgcaagcg ctcgctgtcg gcacgttgtg
      841 ctgacgaggt gacctcgcgg cgactgttgc tcgctgccct ggcgtgtctg cacggcatga
      901 accggcgcga actggcggtg gtgctcagcg aatcggagag caagtggctg ggacaggcca
      961 aggccgtgca cctggacgac ctgccgccgg cggtgcggct ggatttgccc gactggctgc
     1021 atggcgagct gaaccggcaa ttcgccgccg aagccgtcga gcaactggcc gccgcgctca
     1081 atcagccggc gccgctcgac ctgcgggtca atgcgctgaa agcccatcgg caggaggtcc
     1141 tggcgcggct cctggccgac ggcctgacgg ctgcgccgtg cccgtattcg ccactgggca
     1201 tccgcctggc cggcaagccg gcgctgtcca gacacccctt gtttctcgat ggcagcatcg
     1261 aagtccagga tgaaggcagc cagttgctgg gtttcctgct gcaacccaga cgcggcgaaa
     1321 tggtcgccga cttctgtgcc ggtgcgggtg gcaagacgct gctgctcggg gcgatgatgc
     1381 gctcgcaggg ccggctctat gccttcgacg tcgacgaacg gcggctggcg cgactgaagc
     1441 cccgccttgc ccgatccggt ctgtcgaacg tgcacccggt gcgcatcgac tcggagcggg
     1501 acaccaagat ccggcgcctt gccggcaagc tcgatcgggt actcgtcgat gcgccgtgtt
     1561 ccggactggg aaccctgcgt cgcaatccgg acctgaagtg gcggcagaca ccggcgagcg
     1621 ttgcggagtt gtgtaccaaa cagggcgcga ttctcgctgc cgcaggggat ctgctcaagc
     1681 cgggcgggcg cctggtttac gccacctgca gcgtccttga agccgagaac gaagtcatcg
     1741 tccagaattt cctggcgcgc tttcccgagt tcgtcctgtg ctcagcgcag gagatcctgg
     1801 ctcgacagga gattgcgatt gacgttggcg agcggttgcg gctgttgccg caacatcacg
     1861 ggaccgatgc cttttatggc gcagtaatgg agcggaaatg aacgaacaag tcgtttttgc
     1921 cctgctgatc gaattgtggc gggacattcg ggagccggcg ttcctctggc aggtgctggc
     1981 cctgggcat
//
LOCUS       DAOSIT010000464         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49221_length_1988_cov_8.561821, whole genome shotgun sequence.
ACCESSION   DAOSIT010000464 DAOSIT010000000
VERSION     DAOSIT010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49221_length_1988_cov_8.561821"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1988
                     /locus_tag="PLL14_10000"
     CDS             33..>1988
                     /locus_tag="PLL14_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04302.1"
                     /translation="MISFATTVFLSAFLLFQIQPIVAKMILPWFGGSSSVWSTCLVFF
                     QAELLLGYLYVHLLHELLSPRRQNLVHGVLLLLSLATLPVIADPAWKDAAFESPTWSV
                     LVVLASAVGMPYLLLSTTGPLMQAWYARSFASMMPYRLYALSNLASMLALLSYPLLVE
                     PYFPVLQQAWAWSAAYVLFVLACLASAWQSWKGVSSERELAAVTTSTAATARPSWREC
                     LLWVGLAMTASILLLAITRHLTQDVAPVPFLWVLPLSIYLLSFILCFDAPRYYYRPGF
                     LVALPMAFLALDQVVDGGSMPVPLLVALLSLSLFVFCMVCHGELVRRRPAVRHLTLFY
                     LMLSIGGALGGSFVGLLAPAVFNAYFELPIGLFLCALLVIIVLWREIKPVWRWLLLVA
                     LFAYGYRLTGISVSYVEDYRRVLRNFYGQLRVADVDEEDLGIKRKILHGRINHGEQFI
                     SPEHRRQATAYYCEKSGIGQALRSLSGEKPRKIGVLGLGAGTLATYGRQGDEMRMYEI
                     NEQVLDLARNDFSYLGDSAARIVPVLGDGRLMLEREPIQNFDLLAIDAFSGDSIPTHL
                     LTIEAMRAYLRHMPRNGILAVHITNRYLDLRPVVAAAAQQLGMSALVVDFEPEHGERF
                     CRHSVWALIVPNDRQASLLKEIHQAETL"
BASE COUNT          294 a          672 c          595 g          427 t
ORIGIN      
        1 cgacaacatt tcgcgtttct tcgaaagtcc cgatgatcag cttcgccacc accgtcttcc
       61 tgtcggcctt cctgcttttc cagatccagc cgatcgttgc caagatgatc ctgccgtggt
      121 tcggcggctc gtcctcggtg tggagtacct gcctcgtctt cttccaggcc gaacttctgc
      181 tcggctacct gtacgtgcac ttgctgcacg agttgctgtc gccgcgccgc cagaacctgg
      241 tgcatggcgt gctgctgctg ctcagcctgg ctaccctgcc ggtgatcgcc gacccggcat
      301 ggaaggacgc cgcattcgag agcccgacct ggagcgtgct ggtcgtcctg gcctcggcgg
      361 tcggcatgcc ctacctgctg ctgtcgacca ccgggccgct gatgcaggcc tggtatgcgc
      421 gctccttcgc cagcatgatg ccctaccgtc tgtatgccct ctccaacctg gcctcgatgc
      481 tggccctcct gagttatccg ctgctggtcg agccgtattt cccggtcctc cagcaagcct
      541 gggcgtggtc cgccgcctac gtgctgttcg tgctggcttg tctggcgagt gcctggcaat
      601 cctggaaagg ggtatcgagc gaacgagagc tggctgcggt tacgaccagc accgccgcca
      661 cggcgcgccc gtcatggcgc gagtgcctgc tgtgggtggg cctggcgatg accgcctcga
      721 tcctcctgct ggccatcacc cgccacctga cacaggatgt tgcgccggta cccttcctgt
      781 gggtgttgcc gctgagcatc tacctgctga gtttcatcct ctgtttcgat gcgccccgtt
      841 actactaccg ccccggcttc ctggtggccc tgccgatggc cttcctcgcc ctcgaccagg
      901 tcgtcgacgg tggcagcatg cccgtgccgt tgctggtcgc tctgctgtcg ctgtcgctgt
      961 tcgtcttctg catggtctgc catggtgaac tggtcagacg tcggccagcc gttcgccatc
     1021 tgacgctgtt ctatctgatg ctctcgatcg gcggcgcgct cggcggaagc ttcgtcggcc
     1081 tgctggctcc ggcggtattc aacgcctact tcgaactgcc gatcggcctg ttcctctgcg
     1141 ccctgctggt gatcatcgtc ctctggcgcg aaatcaagcc cgtctggcgc tggctgctgc
     1201 tggttgccct gttcgcctac ggctaccgtc tgaccggtat ctcggtcagc tacgtcgagg
     1261 attaccggcg ggtgctgcgc aatttctacg gacaactgcg ggtcgccgac gtcgacgaag
     1321 aagacctggg aatcaagcgc aagatcctgc acgggcgcat caatcacggc gagcagttca
     1381 tctctcccga gcaccgccga caggccacgg cctactactg cgagaaatcg ggaattggtc
     1441 aggcgctgcg cagcctctcc ggagaaaagc cgcgcaagat cggggtgctg ggtcttggtg
     1501 ccgggacgct ggccacctat ggccggcagg gcgatgaaat gcggatgtac gagatcaatg
     1561 agcaggtcct cgacctggcg cgcaatgact tcagctacct tggcgacagc gccgcgcgga
     1621 tcgtcccggt tctcggcgat gggcgcctga tgctcgaacg cgaaccgata cagaatttcg
     1681 acctgctggc aatcgacgct ttctctggag actcgatccc cacgcacctg ctgaccatcg
     1741 aagccatgcg tgcctatctg cgccacatgc cgcggaacgg catcctggcg gtgcacatca
     1801 ccaaccgcta tctcgacctg cggccggtgg tcgcggccgc cgcgcagcaa ctcggcatga
     1861 gcgctctggt tgtcgacttc gaacccgaac acggcgagcg cttctgccgc cattccgtgt
     1921 gggcgctgat cgttccgaac gatcgccagg ccagcctgct gaaggaaatt caccaggccg
     1981 agacgctc
//
LOCUS       DAOSIT010000465         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49257_length_1987_cov_8.037267, whole genome shotgun sequence.
ACCESSION   DAOSIT010000465 DAOSIT010000000
VERSION     DAOSIT010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49257_length_1987_cov_8.037267"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PLL14_10005"
     CDS             complement(<1..1286)
                     /locus_tag="PLL14_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOG04303.1"
                     /translation="MALETEIKLSLPASAARRLPAHGLLADSKPLRQKLVNTYYDTPD
                     RRLQRKRVAVRYRQKGTEWLLTVKSDAPSPGGLAQRSEWEAPGQPGDFDFAHVDNPKL
                     RRFLESVTPELVPLFTTDFTRNFWVVVPQAGTRIEVALDRGKIVAGERQEPICEVELE
                     LLEGDVAALFSAALSLQSDLPLHPEGASKAERGYRLASAEALVPNKATDVRLEMGMTS
                     IAVFRSTAFSCLSQLQGNERGLRESNAPEFIHQARVAMRRLRSAIRLWRPLLPEEYVN
                     NFDPRWRTLANQLGDTRNWDVFITEILPPISKAFPDHSDLQRLVGQAMSHLAGCRKAA
                     QVVMTSPTYSQLLLEFTAATVALEESKKPPLTVFAPRSLNKRAKRVAALALETRDSDP
                     EARHALRVALKRLRYALEFFAPLFPARRLQRYHQSAA"
     gene            complement(1329..>1987)
                     /locus_tag="PLL14_10010"
     CDS             complement(1329..>1987)
                     /locus_tag="PLL14_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG04304.1"
                     /translation="LLAHVPLFNELESEEIVRLARGSRELAASRGDILFHKGDIPTGF
                     YLIVYGQVKLAFTSAQGGEKVVDILGQGQTFGEAVMFMDKPCMVYAQALADSLLLHIA
                     KTAILDELDNDPRLGRKMIAGLSMRLHHLINDVESYSLHSGRQRIIGYLLRDNPVDGD
                     KGATVTLPTNKGVIASRLNLTQEHFSRILHELSEKGLIVVEGRKIHIPDVHRLRAHDQ
                     "
BASE COUNT          350 a          584 c          660 g          393 t
ORIGIN      
        1 gcagcgcttt ggtgatagcg ctgcaggcgg cgtgccggga agagcggtgc gaagaactcg
       61 agtgcgtagc gcagccgctt cagggccacg cgcagcgcgt gtcgggcttc cgggtcggaa
      121 tccctggtct ccaacgccaa cgcggcgacg cgcttggcgc gcttgttcag ggaacgcggt
      181 gcgaatacag tcagcggcgg cttcttgctt tcctccagag cgaccgtggc ggcggtgaac
      241 tcgagcaaca attgcgagta ggtgggcgag gtcatgacca cttgcgccgc cttgcggcag
      301 cctgccaggt gactcatggc ctgaccgacc agacgctgca ggtcgctgtg atccgggaac
      361 gccttgctga tcggaggcag gatctcggta atgaagacgt cccagttgcg cgtgtcgcca
      421 agttgattgg ccagcgttcg ccagcggggg tcgaagttgt tgacgtattc ctcgggcaaa
      481 agcggcctcc agagacggat cgccgagcgc agccggcgca tggcgacacg cgcctggtgg
      541 atgaattccg gggcgttgct ctcgcgcaga ccgcgctcgt taccctgcag ttgcgacagg
      601 cacgagaagg cggtgctgcg gaacaccgcg atgctggtca tgcccatttc caaacgcacg
      661 tcggtggcct tgttcggtac cagggcctcg gcgcttgcca gacggtagcc gcgctcggcc
      721 ttgctggcgc cttcgggatg cagcggcaga tcgctctgca gggacagtgc ggcagaaaac
      781 agtgcggcga catccccctc gagcaattcg agttcgacct cgcagatcgg ttcctggcgc
      841 tcacccgcca cgatcttgcc acggtcgaga gcgacctcga tgcgcgttcc cgcctgcgga
      901 accacgaccc agaagttgcg cgtgaagtcg gtggtgaaga ggggaaccag ttcgggagtt
      961 accgattcga gaaagcggcg cagcttcgga ttgtccacgt gcgcgaaatc gaaatcaccg
     1021 ggttgaccgg gagcctccca ctcgctgcgc tgcgccagtc cgcctggcga aggggcgtca
     1081 ctcttgacgg tcagcagcca ttcggtgccc ttctgccggt agcgcacagc gacgcgcttg
     1141 cgctgcagac gccggtcagg cgtgtcatag taggtgttga ccagtttctg gcggagcggc
     1201 ttgctgtcgg ccagcaggcc gtgtgcaggc agccggcggg cggcgcttgc gggcaggctc
     1261 agcttgatct cggtttccaa tgccatgcgt ggtgtctcct ttatcggttg gtgtaagtgc
     1321 cgcagacttc attgatcgtg cgcgcgtagt ctatggacat cgggaatatg gatttttcgc
     1381 ccttcaacca cgatcagccc tttttcagag agttcgtgca gaatgcgtga gaagtgctcc
     1441 tgcgtcagat tgaggcgcga ggcaatgacg cctttgttgg tcgggagcgt gacggtggct
     1501 cccttgtcgc cgtcgacagg attgtcgcgc agcaggtagc cgatgatgcg ctgccgaccc
     1561 gaatgcagcg agtacgactc cacgtcgttg atcaggtgat ggagccgcat cgagaggccg
     1621 gcgatcatct tgcggccgag tctgggatcg ttgtcgagtt cgtcgaggat ggccgtcttg
     1681 gcgatgtgca gcagcagcga gtccgccagg gcctgcgcat agaccatgca cggcttgtcc
     1741 atgaacatca ccgcttcgcc gaaggtctga ccttgtccca ggatgtcgac caccttttcg
     1801 ccaccttgtg ccgaggtaaa tgccagtttc acctggccgt agacgatcag atagaagccg
     1861 gtcggaatgt ctcccttgtg gaacaggatg tcgccacgcg atgccgcgag ttcacgagaa
     1921 ccgcgcgcga gacggacaat ttcttcactt tccagctcat tgaacagcgg cacatgcgcc
     1981 agtagcg
//
LOCUS       DAOSIT010000466         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49366_length_1984_cov_8.994816, whole genome shotgun sequence.
ACCESSION   DAOSIT010000466 DAOSIT010000000
VERSION     DAOSIT010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49366_length_1984_cov_8.994816"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /gene="cutA"
                     /locus_tag="PLL14_10015"
     CDS             <1..239
                     /gene="cutA"
                     /locus_tag="PLL14_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOG04305.1"
                     /translation="ACVNILPACHSIYRWQGVLETADEVPLWIKTTSERYPALQAAIR
                     NRHPYALPEVIALPVVQGLPDYLAWVARETRIPD"
     gene            286..>1984
                     /gene="dsbD"
                     /locus_tag="PLL14_10020"
     CDS             286..>1984
                     /gene="dsbD"
                     /locus_tag="PLL14_10020"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HOG04306.1"
                     /translation="MLLGTLVCAEEFLDPAIAFKPTVHALDGQTLEIRFEIAKGYYLY
                     RDRFRFAVEPATVELGAPLLPPGKEKRDDTFGDVEVYYHEAIIRLPVVRNTSGPLPLT
                     LVLTSQGCADAGICYPPQQQKLAVQLPDPATVAIVAAAPREGPTGDESGHIAQLFRHA
                     DFWLLIGSFFGFGLLLALTPCVFPMIPILSSIIIGAGRRGRAVSHARGFSLSLAYVLG
                     MAVTYAAAGVAAGLTGTLLAAALQNPWVLGGFAAVFVVLALSMFGFYEVQLPTFLQSK
                     VSEEARHIRGGSLPGVAAMGALSAIIVGPCVAAPLAGALLYIGQTGDAVLGGAALFAM
                     GLGMGVPLLAVGVSTGSLLPRSGPWMEAIKKAFGVVLLATAVWIVSPVIPVVAQMLAW
                     SLLLIVPAIYLHALDPLPPHARGWQRFWKGIGIVLLLLGAAMLLGTLAGSRDPLQPLS
                     VLRGGTASADTRPLAFTRVRSLAELDSRIAAAGQPVMLDFYADWCVSCKEMERFTFAD
                     PRVQARLANWTLLQVDVTANSDDDRALLQRFKLYGPPGIIFFDRSGKEVEGLRVVGFQ
                     "
BASE COUNT          277 a          680 c          651 g          376 t
ORIGIN      
        1 cggcctgcgt gaatatcctg ccggcgtgcc actcgatcta ccgctggcag ggcgtgctcg
       61 aaacggccga cgaggtgccg ctgtggatca agacgaccag cgagcgctac ccggcgctgc
      121 aggcagcgat tcgcaaccgc cacccctacg cgcttccgga agtgatcgcg ctacccgttg
      181 tccagggact gcccgactat ctcgcctggg tcgcccgtga aacgcgcatc cccgactgac
      241 ttctccaaga ttcccgccat gatccgctgg ttgctcgctt tcactatgct gctgggcacc
      301 ctggtgtgcg ccgaggaatt tctcgacccg gcgatcgcct tcaagccgac agtacacgcc
      361 ctcgacggac aaaccctgga aatccgcttc gagatcgcca agggctacta tctctaccgt
      421 gaccggttcc gtttcgcggt cgagccggcg acggtggaac tcggcgcacc gctcctgccg
      481 ccgggcaagg agaagcgcga cgacactttc ggcgacgtcg aggtgtacta ccacgaagcg
      541 atcatccgtc tgccggtcgt gcgcaacacc tcgggaccgc tgccgctgac gctcgtcctg
      601 acctcacagg gctgcgccga cgccggcatc tgctatccgc ctcagcagca gaagctcgca
      661 gtgcagttgc cggacccggc aacggtagcg atcgtcgcgg cagccccgcg cgagggccca
      721 acgggcgacg agtcgggaca catcgcccag ctgttccggc acgccgactt ctggctgctg
      781 atcggcagct tttttggttt cgggctgctg ctggcgctga ccccctgcgt cttcccgatg
      841 attccgattc tgtcgagcat catcatcggc gccggtcgcc gtggccgtgc ggtgtcgcac
      901 gcccgcggct tctcgctgtc gctggcgtac gttctcggca tggccgtcac ctatgcggcg
      961 gccggcgtcg cggccgggct gaccggcacc ctgctggcgg cggcgttgca gaacccctgg
     1021 gtgctgggag gtttcgcagc ggtgttcgtg gtcctcgcgc tgtcgatgtt cggcttctac
     1081 gaagtgcaac tgccgacctt cctgcaaagc aaggtgtccg aggaggcccg ccatatccgc
     1141 ggcggctcac tgcccggtgt cgcggccatg ggcgcactgt cggcgatcat cgtcggaccg
     1201 tgtgtcgccg cgccgctggc cggagccctg ctctacatcg gccagaccgg cgatgcggta
     1261 ctcggcggtg cggcgctgtt cgcgatgggc ctgggcatgg gcgtaccgct gctcgccgtt
     1321 ggcgtctcga ccggcagctt gttgcccaga tccggcccgt ggatggaggc catcaagaag
     1381 gcgttcggcg tggtcctgct ggcgaccgcc gtctggatcg tctcaccggt gattccggtg
     1441 gtcgcccaga tgctggcgtg gtcgctgctg ctgatcgttc cggcgatcta tctgcatgcg
     1501 ctcgatccct tgccgccgca cgccaggggc tggcaacgct tctggaaagg gatcggcatc
     1561 gtcctgctgc tgctcggtgc ggcgatgctg ctgggcacgc tcgccggctc gcgcgatccg
     1621 ctgcagccgc tgtcggtcct gcgcggcggc acggcgagcg ccgacacccg gccactggcg
     1681 ttcacccgcg tgcgctcgct ggccgaactc gacagccgca tcgcggctgc ggggcagccg
     1741 gtaatgctcg atttctatgc cgactggtgc gtgtcgtgca aggaaatgga acgtttcacc
     1801 ttcgccgatc cacgcgtgca ggcccggctg gccaactgga ccttgctgca ggttgatgtc
     1861 accgccaact cggatgacga ccgggcattg ctgcagcgtt tcaagctcta cgggccgccg
     1921 ggaatcatct tcttcgaccg cagcggcaag gaggtcgagg gcctgcgcgt ggtcggcttc
     1981 caga
//
LOCUS       DAOSIT010000467         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49498_length_1980_cov_9.448831, whole genome shotgun sequence.
ACCESSION   DAOSIT010000467 DAOSIT010000000
VERSION     DAOSIT010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49498_length_1980_cov_9.448831"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..947)
                     /locus_tag="PLL14_10025"
     CDS             complement(21..947)
                     /locus_tag="PLL14_10025"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOG04307.1"
                     /translation="MNNAFPQASADCAPLPRTIALVGKYHSPEIAESTRLLAKYLHER
                     GMTVLIEEETARNVVSHLDLGSWASGGFAWLGAHADIAIVIGGDGTMLNAARQLARYR
                     VPLVGVNQGRLGFMTDIARADMLTSLDDLLDGKFIPETRMLLDAEIVRDERGVVSNLA
                     LNDVVVDKGAIGRMIEFELFIDGEFIYHLRSDGLIVATSTGSTAYSLSANGPILHPQV
                     SAIALVPLCPHALTNRPILVGDRNEIEIRIVNATDSRAHFDGQVTADLRNGDVVRIRR
                     SEYSICFLHPPGYSYFAMLRQKLHWSERPKGH"
     gene            1023..>1980
                     /gene="hrcA"
                     /locus_tag="PLL14_10030"
     CDS             1023..>1980
                     /gene="hrcA"
                     /locus_tag="PLL14_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOG04308.1"
                     /translation="MLDDRARTLLKTLIERYIAEGQPVGSRALSRYSGLDLSAATVRN
                     VMADLEDIGLIASPHTSAGRVPTSRGYRFFVDSLLTVKPLEKEKLDAIEGQLLPSQPR
                     QLISNASHLLSGLTHFAGIVLTPRRMAPKIRQMEFVTLSERRLLLILVTSDGDVQNRL
                     LFPGRSYTPSELVTATNYLNQHFVGHDFEHIHSRVQEELRELRGDLQALMTAALAAGD
                     EAMKGPGDRYVISGERNLLEVEEFSSNMRRLRELFDLFEQRTALMQLLDLSKRADGVQ
                     IFIGGESGLAPLDECSVVTAPYEVDGQVVGSVGVIGPTRMAYE"
BASE COUNT          394 a          588 c          606 g          392 t
ORIGIN      
        1 atggccgttg ccagaatggt ctaatgaccc ttgggccgct cgctccagtg cagcttttgg
       61 cggagcatgg cgaagtaact gtagcccggt ggatgcagga aacagatcga gtattcggag
      121 cggcggatac gcacgacatc cccgttccgc agatcagccg tcacctggcc atcgaaatgg
      181 gcacgtgaat ccgttgcgtt aacaatccgg atctcgatct cgttgcggtc gccaaccagg
      241 atcggccgat tggtcagggc atgcgggcag agtgggacca gagcgatggc cgatacctgc
      301 ggatgcagga tcgggccgtt ggcggacagc gaataggctg tcgagccggt tgatgtggcc
      361 acgatcaagc cgtcggagcg caggtggtag atgaactcgc catcgatgaa gagttcgaat
      421 tcgatcatcc gcccgatggc gcccttgtcg acgaccacgt cgttcaacgc cagattggaa
      481 acgacaccac gctcgtcgcg cacgatttcc gcatcgagaa gcatgcgcgt ttcgggaatg
      541 aacttgccgt cgaggaggtc gtccaggctg gtcagcatgt cggcacgcgc aatgtcggtc
      601 atgaagccga ggcgcccctg gttgacgccg accagcggta cgcgatagcg ggcaagctgg
      661 cgtgcagcat tgagcatggt gccgtcaccg ccaatgacga tggcgatgtc ggcgtgtgcc
      721 ccgagccagg cgaaaccacc gctagcccaa ctcccgaggt caaggtgcga aaccacgttg
      781 cgcgcggttt cttcctcgat cagtacggtc atgccgcgtt catgcaggta cttggccagc
      841 aaacgagtcg actcggcaat ctccggactg tggtactttc cgaccagcgc tatcgtcctg
      901 gggagaggcg cgcaatcagc cgaggcttga ggaaaggcgt tgttcatggg acgaattaaa
      961 ccacaattca gcttgcttcc gcgccacccc gtgcgggaag gattttttgt agaatcggaa
     1021 ccatgctcga cgaccgtgcc agaacccttc tgaagaccct gatcgaacgc tacattgcgg
     1081 aagggcaacc ggttggctcg cgcgccctgt ccaggtattc aggcctggac ctttcggctg
     1141 cgacggtacg caatgtcatg gccgacctcg aagatatcgg actgattgcc agcccgcata
     1201 cttccgcagg acgcgtaccg acgtcgcggg gttatcgctt cttcgtcgac agcctgctta
     1261 ccgtcaagcc gctcgagaag gaaaaactcg acgccatcga gggacaattg ctcccttcgc
     1321 agccgcggca actgatcagc aatgcctcgc atctcctttc cgggctgact cattttgcag
     1381 gcatcgtcct gacgccgcga cggatggctc cgaaaattcg ccagatggag ttcgtcacgc
     1441 tctcggagag gcgcctgctg ctgatcctgg tgacttcaga tggcgacgta cagaatcgcc
     1501 tgcttttccc cgggcgctcc tataccccgt ccgaactggt caccgccacc aactatctga
     1561 atcagcattt tgtcggccat gacttcgagc atatccattc ccgcgtccag gaagaattgc
     1621 gcgagttgcg cggtgacttg caggcgctga tgacggcggc gctggccgcc ggcgacgagg
     1681 cgatgaaggg gccaggcgac cgatatgtca tctcgggtga gcgtaacctg cttgaagtcg
     1741 aggaattttc ctccaacatg aggcgccttc gcgagttgtt cgacctcttc gaacagcgca
     1801 ccgcgctgat gcagttgctg gacctttcga agcgcgccga tggggtacaa atcttcatcg
     1861 gcggggaatc gggactggct cctctggacg aatgcagcgt ggtcaccgcg ccctacgagg
     1921 ttgacggcca ggtggtcggt tcggtaggcg tcatcggccc cacccgcatg gcctacgagc
//
LOCUS       DAOSIT010000468         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_49878_length_1969_cov_8.848485, whole genome shotgun sequence.
ACCESSION   DAOSIT010000468 DAOSIT010000000
VERSION     DAOSIT010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49878_length_1969_cov_8.848485"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLL14_10035"
     CDS             <1..396
                     /locus_tag="PLL14_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOG04309.1"
                     /translation="INWAGKDTLFRWPMKRFFIWLGGVPINRRENTGMIGQLIDLFAQ
                     SERFCMCITPEGTRSRSDHWKTGFYRLALATGVPVGLGFIDYGNKRIGVERWISLSGT
                     EETDLALFRSYYADKTGRYPEKAGEIRFK"
     gene            complement(496..1875)
                     /locus_tag="PLL14_10040"
     CDS             complement(496..1875)
                     /locus_tag="PLL14_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOG04310.1"
                     /translation="MDRNTRNVFQQRQRHVGRRMRATEAVEFFNVLTSPELLETTEAL
                     LPEYRERLYPPTVALSMFMRQVLEADGSCQKAVNGWAAQRAADGLAPCSVLTGGYCRA
                     RKRLPLEMVSTLARETGRLLSEKALDPWLWRGRTVKLVDGTGISMPDTEQNQARYPQP
                     SSQAPGVGFPQARLVMVICLASGAALDMAVGPYSGKGSGELGLVRAMLEGFSAGDVML
                     ADALYCNYFLIATLIDAGVDVLFEQNGSRITDFRRGQALGTRDHLVYWPKPAARPEWM
                     TPEQYAQFPDELRVREVKVAHQVLVTTMLDHRQVSKDDLSALFGRRWNVELDLRNLKT
                     TTGMDVLSCQTPQMNDKQLWVHLLAYNVIRLLMAQAACNAGIDPRQLSFKHTVQLWTE
                     WVSRGLSATKDCGRLFSLIAQCRVGNRPGRIEPRMRKRRPKPYPWLKIPRAEARRKIE
                     LHGHARETK"
BASE COUNT          382 a          617 c          577 g          393 t
ORIGIN      
        1 atcaactggg cgggcaagga cacgctcttt cgctggccga tgaagaggtt tttcatctgg
       61 ctcggcggcg tgccgatcaa ccggcgcgaa aataccggaa tgatcgggca actgattgac
      121 ctttttgcgc agagcgagcg cttctgcatg tgcatcacgc cggaaggaac gcgttcaaga
      181 agcgatcact ggaaaaccgg tttttaccgg ctggcgctgg cgaccggcgt gccggtcggg
      241 ctgggcttca tcgactacgg caacaagcgt atcggcgtcg aacgctggat cagtctcagc
      301 ggaaccgagg agaccgatct cgccctgttc cgctcctact acgccgacaa gaccggccgc
      361 tatcccgaaa aagccggcga aatccgcttc aagtagcaga actcgcagaa tttgtagaat
      421 ttgtaggtca ggttagcgcc agcgtaaccc gacgtttcga ctgtcgggtt actctggcgc
      481 taacccgaat ggcacttact tagtttcccg cgcgtgacca tggagctcta tctttcgtcg
      541 ggcctcggcg cgtgggatct tcaaccaggg gtagggtttg ggtcgtcgct ttcgcattcg
      601 gggttcgatg cgtccaggcc gattgcccac cctgcattgg gcgatcagcg agaagaggcg
      661 cccacagtcc ttggtcgccg agagaccacg cgagacccac tcggtccaca gttgcaccgt
      721 gtgcttgaaa ctcagttgcc gcggatcgat gccggcattg caggcggcct gcgccatgag
      781 cagtcggatc acgttgtagg ccagtagatg aacccacaac tgtttgtcgt tcatctgcgg
      841 cgtctggcag ctcagcacgt ccatgccggt ggtcgttttg aggttgcgca agtcgagttc
      901 gacgttccat cgccgcccaa acaacgccga gagatcgtcc ttgcttacct gccgatgatc
      961 gagcatcgtg gtgaccagca cctggtgggc cactttgact tcgcgtactc tgagttcatc
     1021 ggggaattgc gcatactgct ctggtgtcat ccactctggg cgcgccgcag gcttgggcca
     1081 gtacacaagg tgatcacgcg tgcccagcgc ctgaccacgg cgaaagtcgg tgatgcgcga
     1141 tccattttgt tcgaacaaca catccacccc ggcgtcgatc agcgtggcga tcaggaaata
     1201 gttgcagtac agggcatcgg cgagcatcac atcacctgcc gagaagcctt cgagcatcgc
     1261 gcgtaccaac ccgagttcgc cgctcccttt gccgctgtaa ggtcctactg ccatgtcgag
     1321 cgccgcgccg ctggccaggc aaatcaccat gaccagtcgc gcctgcggaa aaccgacccc
     1381 gggggcttgg gaactgggct ggggataacg ggcctgattc tgctcagtat cgggcatcga
     1441 gatccccgtg ccgtccacca gcttcaccgt gcgcccccgc caaagccatg ggtccagcgc
     1501 cttctcgctg agcagacgac cggtttcgcg cgcaagcgta ctgaccatct ccaacggcag
     1561 gcgcttgcgc gctcggcagt acccgccggt aagaacgctg caaggcgcga gcccatcggc
     1621 cgcgcgctgc gctgcccagc cgttgaccgc cttctggcac gagccatcgg cctccagcac
     1681 ctggcgcatg aacatcgaca gcgccaccgt cggcggatac aagcgctcgc ggtactcggg
     1741 cagcaacgct tcggtcgtct cgagcaactc aggactcgtc aggacgttga aaaactctac
     1801 cgcttcggtc gcccgcatcc ggcgtccgac gtggcgctgg cgttgctgaa aaacgtttct
     1861 ggtattacga tccatgacgg ctgaccttcc gaaagatggt ttgggtgcgt gagaacaacc
     1921 atcctatcaa tccttggggc agccacctcc ttctcgcctc aaattcaaa
//
LOCUS       DAOSIT010000469         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50166_length_1961_cov_8.210913, whole genome shotgun sequence.
ACCESSION   DAOSIT010000469 DAOSIT010000000
VERSION     DAOSIT010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50166_length_1961_cov_8.210913"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="rtcA"
                     /locus_tag="PLL14_10045"
     CDS             <1..948
                     /gene="rtcA"
                     /locus_tag="PLL14_10045"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOG04311.1"
                     /translation="LTLSMITGQPFRLENLRAGRPKPGLLRQHLTAVLAAAEICGARV
                     EGAAPGSRTLIFVPSAIRSGDYRFAIGTAGSCTLVLQTILPALWYADGPSTLRISGGT
                     HNPAAPPADFLLRTWLPLVRRMGVQSEIALQRHGFYPAGGGEVHARVAPGRPVAIDLL
                     TRGDWRATRATAIVAGVPEAVAQRELAQLARTLPQVSGEIRDLPEREGPGNALLVEVE
                     HAFLSEVFTAFGARGLAAEKVADQVAAKVRAYLDSPAAVGEHLADQLALPMALAGGGS
                     FTTGQPSSHLTTNLAVIERFLPLRSQIEYAEGRWLVGMG"
     gene            complement(1186..>1961)
                     /locus_tag="PLL14_10050"
     CDS             complement(1186..>1961)
                     /locus_tag="PLL14_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04312.1"
                     /translation="PFSIGFRIEHPQALIDKARFGAQAGNPLLGAADYKLVHHCRNGR
                     SVYSFCMCPGGTVVAATSEANRVVTNGMSQYSRNERNANAGIVVGITPLDYPGGPLAG
                     IELQRELEARAFQLGGGGYAAPAQLVGDFLAGRASTSLGSVIPSYQPGVHLTDLATAL
                     PGYAIAAIREALPAFERQIKGFAMADAVLTGVETRTSSPLRITRGDDCQSLNVRGLYP
                     AGEGAGYAGGILSAGVDGIKVAEAVTRAMLKAGPACASG"
BASE COUNT          314 a          666 c          649 g          332 t
ORIGIN      
        1 ctgacgctgt cgatgatcac cggccagccg tttcgtctgg aaaacctgcg cgccgggcgg
       61 ccgaaacccg gactcctgcg ccagcacctg acagcggtcc tggccgccgc cgaaatctgc
      121 ggtgcgcgtg tcgaaggtgc cgcgcccggc tcgcggacct tgattttcgt accgtcggca
      181 atccgcagtg gcgactaccg tttcgccatc ggcacggccg gcagttgcac gctggtcctg
      241 cagacgatac tgccagcctt gtggtacgcc gacggaccgt cgacgctccg catcagcggc
      301 ggcacccaca atccggcagc gccgccggcc gattttttgc tgcgtacctg gctgcccctg
      361 gtgcggcgga tgggagtgca gagcgaaatc gcgctgcagc gccacggctt ctacccggcg
      421 ggcggtggcg aggtacatgc ccgtgtcgca cccggccggc ccgtcgccat cgatctgctt
      481 acccgtggcg actggcgggc gacgcgtgcc accgcaatcg ttgccggcgt ccccgaggcg
      541 gtcgcgcagc gcgagctggc gcagctcgcg cgcacgctgc cgcaagtcag tggcgagatt
      601 cgcgacctgc ccgagcggga agggcccggc aacgcgctgt tggttgaggt cgagcacgcc
      661 ttcctgagcg aagtgttcac cgctttcggc gcgcgcggac tggctgccga aaaagtggcc
      721 gaccaggttg cggccaaggt ccgcgcctac ctcgacagcc cagccgccgt tggcgaacac
      781 ctcgccgacc agctcgccct gccgatggcg ctggccggcg gtggcagctt cacgaccggt
      841 caaccgtcta gccacctgac gaccaatctc gcggtgatcg agcgcttcct gccgctgcga
      901 agccagatcg aatacgccga aggtcgctgg ctggtcggaa tgggctaaaa tccgccgtca
      961 gccgcaacct cttttcaccc cgccgacgag ttggggttgt cctttgccgc tgcggccaac
     1021 tgcctctcga aagaacctca aaccgatgcc agccatcctc ctggaacttg cccatgacct
     1081 gcagtccgtc cgccgccctg gcgacttttt tgccagcggc aggtgcctgt tgaaccgggc
     1141 gcgtttgagt ttaccgacgg caatctgatc ggctgaaccg cggactcagc cgctggcgca
     1201 cgcaggccct gccttgagca ttgctcgcgt caccgcctcg gccaccttga tgccgtcgac
     1261 accggccgaa agaatcccgc cggcgtaacc cgcaccttcg ccggcgggat atagccccct
     1321 gacgttcagg ctctgacagt cgtcgccgcg cgtgatgcgc aagggcgaag aggtgcgcgt
     1381 ctcgacgccg gtgagcaccg catcggccat cgcaaagccc ttgatctggc gctcgaaggc
     1441 cggcagcgct tcgcggatcg cggcgatggc gtagcccggc aaggcggtcg ccaggtcggt
     1501 cagatggacg ccgggttgat acgagggaat caccgatccg agcgaggtcg atgcgcggcc
     1561 ggcaaggaag tcgccgacca gttgcgcggg tgccgcgtag ccgccaccgc cgagttgaaa
     1621 agcgcgcgct tcgagttcgc gctgcagttc gataccggcc aggggtccgc ccggatagtc
     1681 gagcggggtg atgccgacga cgataccggc gttggcgttg cgttcgttgc gtgagtactg
     1741 gctcatgccg ttggtcacca cccggttcgc ctccgatgtg gcggcgacga ccgtaccgcc
     1801 gggacacatg cagaagctat agaccgaacg accgttgcgg caatggtgca ccagcttgta
     1861 gtcggcggca ccgagcagcg ggttgccggc ctgtgctccg aaacgtgcct tgtcgatcag
     1921 cgcttgcggg tgttcgatac gaaagccgat cgagaagggt t
//
LOCUS       DAOSIT010000470         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50284_length_1958_cov_8.891224, whole genome shotgun sequence.
ACCESSION   DAOSIT010000470 DAOSIT010000000
VERSION     DAOSIT010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50284_length_1958_cov_8.891224"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLL14_10055"
     CDS             complement(<1..701)
                     /locus_tag="PLL14_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOG04313.1"
                     /translation="MDRREFMVSAGIAGTASLLGCRGTPTPLPPGELLGASLALGHRL
                     PAHDLAPPDSTQHVPVLIVGAGIGGLSAGWKLAKSGFADFLIVDLESEAGGNSRAGRN
                     TVSAYPWGAHYLPLPTREATAVRELLAELGVLQGDPRAVRPSYDERYLCATPQERLYR
                     NGRWQEGLLPQSGVGASERGQYQRFADLMAAFRRQRDTLGRRAFALPMALSSRSAELL
                     ALDQLSMRDWLLAQRL"
     gene            complement(705..>1958)
                     /locus_tag="PLL14_10060"
     CDS             complement(705..>1958)
                     /locus_tag="PLL14_10060"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOG04314.1"
                     /translation="AGLFFVFAWSSAPFKLALYLAVVGIGVLVGLEIPLIMRILERDL
                     AFKDLVSQVLTFDYLGALAVSILFPVVLVPHLGLMRSALLFGLLNVAVALWACWLFRE
                     QLRNVGWLGAQGGASMLLLLAAFAAAGELTSLAEAHLYADDIVHAESTPYQRIVVTRW
                     KDDLRLHLNNNLQFSSRDEYRYHEALVHPGLSTLPAARRVLVLGGGDGLALREILRYP
                     QIESVTLVDLDPAMTRLFASAPPLRKLNEDALRSSKVRVVNADALQWLEDNDELFDFV
                     VVDFPDPSNFAIGKLYSASFYRLLEKHLTANALAVIQSTSPLYARQSFWCVVTTLESV
                     GLLVTPYHALVPSFGEWGFVLAGRHAYRPPQAYAVATRFLTADTTPALFLFPKDMARV
                     ETEVNRLNSQVLVRYFEQEWRQVIR"
BASE COUNT          414 a          637 c          627 g          280 t
ORIGIN      
        1 agtcgctgcg ccagcagcca gtcgcgcatg ctcaactggt cgagcgccag cagttcggcc
       61 gaacggctcg acaacgccat gggcaaggcg aaggcacgcc gcccgagagt gtcgcgctgg
      121 cgtcggaaag ccgccatcag gtcggcaaag cgctgatact gtccgcgttc cgaggcaccg
      181 acgccgctct gagggagcag gccttcctgc cagcggccgt ttcgatagag ccgctcctgc
      241 ggagtggcgc acagatagcg ctcgtcatag ctcggtctga cggcacgcgg atcgccctgc
      301 agcacgccca gttcggcaag caactcgcgt accgccgtcg cttcgcgcgt cggcagcggc
      361 agataatgag cgccccaggg gtaggcactg accgtgttgc gaccggcccg agaattgccc
      421 ccggcttcgc tctccaggtc aacgatcaag aaatcggcga agcccgactt ggccagtttc
      481 cagccagcgg aaaggccgcc gatgccggca ccgacgatga gcaccgggac gtgctgcgtg
      541 ctgtcgggtg gcgccaggtc atgcgcaggc aagcgatgcc cgagggcgag cgaagccccg
      601 agcagttcgc ctggcggcag gggcgttggc gtgccgcggc agcccagcag cgaggccgta
      661 ccggcgatcc ccgcagagac catgaattcg cggcggtcca tgccctagcg aatcacctgc
      721 cgccactcct gttcgaagta gcgcaccagc acctgactgt tcagacggtt cacctcggtc
      781 tcgacacgcg ccatgtcctt gggaaacagg aacagggccg gagtggtatc ggccgtcagg
      841 aagcgcgtgg ccaccgcgta cgcctgcggt ggccgatacg cgtgtcgccc cgccagcacg
      901 aaaccccatt caccaaagga cggcaccagc gcatggtagg gtgtgacgag cagcccgaca
      961 ctctccagcg tggtgaccac acaccagaac gactgccggg cgtagagcgg cgaggtggac
     1021 tggatgaccg ccagcgcatt ggccgtcagg tgtttctcga gcaggcgata gaaggaagca
     1081 ctgtagagct tgccgatcgc aaaattcgat ggatcgggaa aatcgacgac gacgaaatcg
     1141 aagagctcat cgttgtcctc gagccactgt agcgcatcgg cattgaccac gcggaccttg
     1201 ctcgaacgta gcgcatcctc gttgagcttg cgtaacggcg gtgcgctggc aaacagccgt
     1261 gtcatcgccg gatcgaggtc gaccagcgtc accgactcga tctgcggata cctgagaatc
     1321 tcccgcaagg ccaaaccgtc gccgccaccg agaaccagca cgcgccgcgc cgccggcaaa
     1381 gtggacagtc ccggatgcac cagtgcctcg tgatagcggt actcgtcgcg cgaactgaac
     1441 tgcaggttgt tgttcagatg caggcgcaag tcgtccttcc agcgcgtgac gacgatccgc
     1501 tggtacggcg tactctcggc atgtacgatg tcatcggcgt agaggtgggc ctcggccagg
     1561 gaagtcaact cgccggccgc ggcgaaagcg gccagcagca ggagcatcga cgcgcccccc
     1621 tgtgcgccta gccagccgac gttgcgcaac tgctcgcgaa acagccagca ggcccagagc
     1681 gcgaccgcaa cgttgagcag gccgaacagc agggcactgc gcatcaggcc cagatggggc
     1741 accagcacga caggaaagag aatcgaaaca gcgagcgctc cgaggtaatc gaaggtcaat
     1801 acctgcgaaa ccagatcctt gaacgccaga tcgcgttcga gtatgcgcat gatcagcgga
     1861 atttccagcc cgacgagcac gccgatgccg acgaccgcca gatacagcgc cagcttgaag
     1921 ggcgccgacg accaggcgaa gacaaagaac aaccctgc
//
LOCUS       DAOSIT010000471         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50615_length_1948_cov_9.432118, whole genome shotgun sequence.
ACCESSION   DAOSIT010000471 DAOSIT010000000
VERSION     DAOSIT010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50615_length_1948_cov_9.432118"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>1948)
                     /locus_tag="PLL14_10065"
     CDS             complement(11..>1948)
                     /locus_tag="PLL14_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOG04315.1"
                     /translation="GLLKMAESLRLMLSANRNFRVTPEGPPEVQLLAQATNDLAQQRD
                     GLMDDVDAQIAAAKSSVEEEKNRLAALMSELAQAVVVCNRDGRILLYNSRARLQFSGL
                     AQAGAALASGALIGLGRSIFSILERNQIDHALEVVRQRLHKGAAAAIANFITTARGGQ
                     LLRVQLVPVLSSRDESQQREMSGYVLTVENITRSLEQESRRDQVLQSLTDGSRGCLAN
                     IRVAVGNLMDYPDMELEIRERFVSIISDEVVRMSQRLDQTMTEFADSLKTRWPLEDVL
                     GIDLIAAAQRRIEARLKLPSKMEEIDEGLWLRAESFSLVFSICFLASRLIDHYQIKEL
                     RFRLLPFGKLAYLDIIWAGHAMSSETFYTWETDAMQVGNESSPLSLRDVIDRHGGEIW
                     YDREKAAHRAFFRFVLPVVPPENMATGESVDVDDVRPEYYDFDLFHFRDQSADLDRPL
                     SDLVYTVFDTETTGLEPAAGDEIIQIGALRIVNGRLLRNENFNQLVDPERFLRPEGTR
                     IHGITDDMVRGQPHIDVVLPAFHDFCSDTVLVAHNAAFDMRFLQMKEERTGVVFSQPV
                     LDTLLLSGVLHANQDSHQLDSIAERLGVSVTGRHSALGDACTTGEVFLKMLPLLAQIG
                     IVTLRQALEASEKTYFARVKY"
BASE COUNT          378 a          577 c          602 g          391 t
ORIGIN      
        1 tccggagggt tcagtacttc acgcgcgcaa agtaggtctt ttcggaagcc tccagtgcct
       61 gccgcagcgt gacgattcca atctgagcca gcagtggcag cattttcagg aatacctctc
      121 cagtggtgca ggcatcgccc agcgcactgt ggcggccggt gaccgaaacg cccaaccgtt
      181 cggcgatgct gtcaagctgg tgagagtcct ggttggcatg caacacaccc gagagcaaca
      241 gggtatcgag taccggctgg ctgaagacga caccggtgcg ttcctccttc atctgcagga
      301 agcgcatgtc gaaagccgcg ttgtgcgcga cgagcacagt gtcgctacag aaatcatgga
      361 aggcgggcag caccacgtcg atgtgcggct gaccacgcac catgtcgtcc gtgatgccgt
      421 ggatgcgggt tccttcgggg cgcagaaagc gctctggatc gaccagttga ttgaaatttt
      481 cgttgcgcag gaggcggccg ttgacgatgc gcagcgcgcc aatctggatg atttcgtcgc
      541 cagctgcggg ttccaggccg gtggtctcgg tatcgaagac cgtgtagacg aggtccgaga
      601 gtggtcgatc caggtcggcg ctctgatccc gaaaatggaa caggtcgaag tcgtaatact
      661 ccgggcgcac gtcatcgacg tcgacgcttt ctcctgtggc catgttctcc ggaggcacga
      721 cgggcaggac aaagcggaag aacgcacgat gtgcagcctt ttcgcggtcg taccagattt
      781 cgccgccgtg gcggtcaatg acgtcgcgca acgacaaggg tgaactctcg tttcccacct
      841 gcatggcatc cgtttcccag gtgtaaaagg tctccgatga catggcgtgc ccggcccaga
      901 tgatgtcgag ataggccagc ttgccgaagg gcagcagccg gaagcgcaat tccttgattt
      961 gataatgatc gatcagacgg gaggcaagga agcagatcga gaataccagg gagaagctct
     1021 ccgctctcag ccacagccct tcgtcgatct cttccatctt gctcggcagt ttcagccgtg
     1081 cttcgatgcg gcgctgggcg gcggcgatga ggtcgattcc gaggacgtct tcgagtggcc
     1141 agcgggtttt cagcgaatcg gcaaactcgg tcatcgtctg atcgagacgc tgactcatgc
     1201 gcacgacttc atcactgatg atgctgacga agcgctcgcg tatctccagt tccatgtccg
     1261 ggtagtccat caggttgccg acggccaccc ggatgttggc caggcaaccc cggctgccgt
     1321 cggtcagcga ctgtagcacc tgatcacggc gcgattcctg ttccaggctg cgggtgatgt
     1381 tttccacggt gagcacgtaa ccgctcatct cgcgctgctg ggattcgtcg cgggacgaca
     1441 gtaccggtac cagttgaaca cgcaaaagct gcccgccgcg ggcagtcgta atgaaattgg
     1501 cgatcgctgc tgctgcgcct ttatgcaatc gctggcgcac gacctccagc gcgtggtcga
     1561 tctgattgcg ttcgagaatc gagaagatcg aacgtccgag gccgatcagc gcgccgctgg
     1621 ccagggctgc gccagcttgt gccagaccgc tgaattgcag gcgcgcgcgg ctgttgtaga
     1681 gcaggatgcg accatcacga ttgcagacca ctaccgcttg cgccagttcg gacatcagcg
     1741 cggccaggcg gttcttctct tcctcgaccg acgacttggc agcggcgatc tgggcatcga
     1801 catcgtccat cagaccatcg cgctgctgcg ccagatcatt cgtcgcctgg gccagcaact
     1861 ggacctccgg tggaccctcc ggcgtcactc tgaaattgcg gttggccgac agcatcaggc
     1921 gcaggctctc ggccatcttg agcaatcc
//
LOCUS       DAOSIT010000472         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50699_length_1946_cov_6.491274, whole genome shotgun sequence.
ACCESSION   DAOSIT010000472 DAOSIT010000000
VERSION     DAOSIT010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50699_length_1946_cov_6.491274"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLL14_10070"
     CDS             <1..703
                     /locus_tag="PLL14_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG04316.1"
                     /translation="RFEFDDKEKLHRQAVEVENLTFAYPGSARKIFDKLNLTINGGDR
                     LAIIGENGVGKSTLLKLLMAELTPQRGTIKWAEKARHGYYAQDHAQDFSSDTNLTDWI
                     AGYARANAADGDDLETLIRGTLGRLLFSGNEVRKAVKVISGGEQGRMIFGKLMLMKPN
                     VLVMDEPTNHLDMESIESLNTALEKFKGTLVFVSHDREFVSSLATRVLEVRLDGKVVN
                     YPGNYEEYLASQGIA"
     gene            complement(713..1297)
                     /locus_tag="PLL14_10075"
     CDS             complement(713..1297)
                     /locus_tag="PLL14_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04317.1"
                     /translation="MRSGQVLLFSLWVSCAAWAAPPEAAPEVPQTLEAAAEQRARAAS
                     MRAEAERRYQADQDHCYTKLLVNDCLVAAKKRYTASIIEARKLDQPARDFERQAKRQE
                     VEVKETQRAADQQQREANQQENAERHRTEEAAKASAREKKLADKARKAEEGQKKTAAE
                     QARRQAKLQKRAQQDAEREARKAAREGSKPAPAN"
     gene            complement(1423..1593)
                     /locus_tag="PLL14_10080"
     CDS             complement(1423..1593)
                     /locus_tag="PLL14_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04318.1"
                     /translation="MAVCRQNGVESTFFKRGEQFMVRTGGPDGKLADYEVRYTLCVDP
                     LQQSLIDALRQQ"
     gene            complement(1633..>1946)
                     /locus_tag="PLL14_10085"
     CDS             complement(1633..>1946)
                     /locus_tag="PLL14_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04319.1"
                     /translation="KAVRADGERLTMDQLSTGRRDQLFLALRLAAIEGHLENGEPLPV
                     IVDDILIQFDDEAAAATFQVLADLSLRTQVLFLTHHEHLLNVARAAIGDDAYRVQRLS
                     P"
BASE COUNT          344 a          648 c          600 g          354 t
ORIGIN      
        1 ccgcttcgag ttcgacgaca aggagaagct gcaccggcag gcagtggagg tcgagaacct
       61 gactttcgcc tatccgggca gcgcgcgcaa gattttcgac aagctgaacc tgacgatcaa
      121 cggcggcgac cggctagcga tcatcggtga aaacggcgtc ggcaagagca ccttgctgaa
      181 gctgctgatg gccgagttga cgccacagcg cggcaccatc aagtgggccg agaaggcacg
      241 ccacggttac tacgcccagg accatgcgca ggatttcagc agcgacacca acctgaccga
      301 ctggatcgcc ggctacgccc gtgccaacgc ggccgacggc gacgacctcg aaacgctgat
      361 ccgcggcacg cttggacgtc tgctgttctc gggcaacgaa gtgcgcaagg cggtcaaggt
      421 aatctcgggt ggtgagcagg gccgcatgat cttcggcaag ctgatgctga tgaagccgaa
      481 cgtgctggtc atggatgagc ccaccaacca cctggacatg gaatcgatcg agtcgctcaa
      541 caccgcgctc gaaaagttca agggtacgct ggtcttcgtt tcgcacgacc gcgagttcgt
      601 ctcgtcgctg gccacgcgtg tcctcgaagt gcgcctcgat ggcaaggtgg tcaactaccc
      661 gggcaactac gaagagtacc tggcaagcca gggcatcgcc tgacacacgc gatcagttgg
      721 cgggagccgg cttgctgccc tcgcgggcag ccttcctggc ctcgcgctcg gcgtcctgct
      781 gggcacgctt ctgcagtttc gcctgccgcc tggcctgctc ggcggccgtt ttcttctgcc
      841 cctcttcagc ctttctcgcc ttgtcggcaa gcttcttttc gcgcgcgctg gccttcgcgg
      901 cttcctcggt ccggtggcgc tcggcgttct cctgctgatt ggcctcgcgt tgctgttgat
      961 cggcggcgcg ttgggtttcc ttgacttcga cttcctgccg cttcgcctgc cgttcgaagt
     1021 cgcgagccgg ctgatcgagc ttgcgcgcct cgatgatgga cgccgtataa cgcttcttgg
     1081 ccgcaaccag gcaatcgttg accagcaact tcgtgtagca atggtcctga tcggcctgat
     1141 aacggcgctc cgcctcggcg cgcatcgacg ccgcccgcgc ccgctgctct gcggcggctt
     1201 cgagcgtttg cggcacctcc ggcgcggcct ccggtggcgc cgcccaggcc gcgcaactta
     1261 cccacagcga aaacaacagc acctgtcctg aacgcatgga atcctccttg tacacccaca
     1321 tgccgcggcc cggcaaaccc gtgcactata agcgacgcgg ccgtttgcgt ggcagcagcg
     1381 ccggccacca ccggcgacgg tcggtcaacg gcggcggtag ccttactgtt ggcgcaaggc
     1441 atcgatcagg gactgctgca gcggatccac acagagggtg taacgcactt cgtagtcggc
     1501 caacttgcca tccggaccgc cggtgcgcac catgaactgc tcgccacgct tgaaaaaggt
     1561 cgactcgacg ccgttctgtc tgcataccgc caaacgcgcc ggccaaacgc ctcgccgcat
     1621 gtgcccgccg gatcacggcg acaggcgctg tacccgatac gcgtcgtcac caatggccgc
     1681 ccgcgccaca ttgagcaggt gctcgtggtg cgtcaggaac agcacctgcg tgcgcagaga
     1741 gagatcggcc agcacctgga aagtcgctgc cgcagcctcg tcgtcgaact ggatcaggat
     1801 gtcatcgacg atcaccggca agggctcgcc gttttcgagg tgcccttcga tggcggccag
     1861 gcgcaaggcg agaaagagct gatcgcggcg accggtgctc agttgatcca tcgtcagacg
     1921 ctcgccatcg gcgcgaacgg ctttga
//
LOCUS       DAOSIT010000473         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50893_length_1941_cov_7.971898, whole genome shotgun sequence.
ACCESSION   DAOSIT010000473 DAOSIT010000000
VERSION     DAOSIT010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50893_length_1941_cov_7.971898"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..943
                     /locus_tag="PLL14_10090"
     CDS             398..943
                     /locus_tag="PLL14_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOG04320.1"
                     /translation="MRYIRILGLLACTLAPAVSLAQAEKVLRADEINERALVDLLAPE
                     KAARPKTRSINVIRDESSLAPAKPAAANLLIIFETNSAGLTPTGKQSLDVVGRALNSE
                     KLVPFSFAIEGHTDPRGGDEYNRQLSQSRAESVVGYLSQNHQIDRSRLKPVGKGPSQL
                     LNPARPEAPENRRVTIRRLDN"
     gene            complement(984..>1941)
                     /locus_tag="PLL14_10095"
     CDS             complement(984..>1941)
                     /locus_tag="PLL14_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOG04321.1"
                     /translation="EDRPRSVAEFRALLAVQDSPGTPAGNSSPAASPRAPAAVAATAA
                     ATKGMGKAIWLVGIAGTALAIAGGAFYTLRSAPDAPSRQLEVAQAKSSPETPPAAAGT
                     AVPQSTPGAELQTVDRKSVNELQLVDMLLAKNGTTNPSGNAAKPFDPVDVLQDIYRQR
                     DHEHVVTVVPEKAKVRIGQDKLRFRITSLKPGYVYILMVGTDNNHFNLLFPNALDADN
                     GIAGDGDLSLPRPGWAMTAAGPPGSNRFVVIVSENRRDFSAAGLRKVDPFAEFPLKLA
                     ERIAREARAEGGNPFAGKVVCPTGRTCSSSYGAALFVIDEVD"
BASE COUNT          349 a          626 c          603 g          363 t
ORIGIN      
        1 gtggtgacgc cttcttcggc gatctgctgg tgcaggcggg tgattttcac ctggcgccgc
       61 ccggcagtga gcacggcgtg acgaccactg atggcggcgc tctggctttc ttgcgcggcc
      121 gcgagatatt gcccctgccg cgccgggctt aacccgcgtt ttatcctgtt atgtgcttgc
      181 gctacaggga acggcgtatg ctatgcgaac cgtcccggta tgccggagag agaatgcgcc
      241 cgattcggga cggccaggca tccccgactt ggccgaggca gtcttgctgt tgcacaatga
      301 cagttttcct gcacgcgccc gagtgcccag gcgggccggt ccggtaaggg tgctcagagc
      361 gccaagttca agtcaatcgt gttcacggag ttgtcacatg agatacatca ggattctggg
      421 attgctggcc tgcacactgg ccccggcggt ttcgctcgca caggctgaaa aagtgctccg
      481 ggcagacgaa atcaacgaac gcgcgctggt tgatctgctt gcccccgaaa aagcggcgcg
      541 gccgaaaacg cgttcgatca acgtcattcg ggacgagagt tcgctggcgc ctgcgaagcc
      601 ggcggcagcg aacttgctga tcatcttcga gaccaactcc gcgggcctga cgcccaccgg
      661 caagcagtca ctcgatgtgg tcggtcgcgc cctgaactcg gaaaaactgg taccgttcag
      721 cttcgccatc gaaggccaca ccgatccacg tggtggcgac gaatacaacc ggcagttatc
      781 gcaatcgcgc gccgaaagcg tcgtcggcta cctttcgcag aatcaccaga tcgatcgcag
      841 ccgtctcaag ccggtcggca aggggccgtc gcaactgctt aatccggccc gcccggaagc
      901 ccccgaaaac cgtcgcgtga cgatcagacg actcgacaac taagcgccat cggctttgcc
      961 ggggagcctt ggttccgagc cggtcagtcg acctcgtcga tcacgaacag cgctgcgccg
     1021 tagctcgacg agcacgtccg gccggtggga cagaccacct tgccggcaaa cggattgccg
     1081 ccttcggcac gcgcctcgcg cgcgatccgt tcggcaagct tgagcgggaa ctcggcaaac
     1141 ggatcgactt ttctcagccc ggcagcagag aaatcccgac ggttttccga gacaatgacc
     1201 acgaagcggt tgctccccgg cgggccggct gcggtcatcg cccagccggg gcgaggcagg
     1261 ctcaggtcgc cgtcgccagc gatcccgttg tcggcatcca gggcgttcgg aaacagcagg
     1321 ttgaagtggt tgttgtcggt accgaccatc agaatgtaca cgtagcctgg cttgagcgag
     1381 gtaatgcgga accgcagctt gtcctgacct atcctgactt tcgctttctc cggcaccacc
     1441 gttaccacgt gctcgtggtc ccgctggcga tagatgtcct gcagcacgtc gaccgggtcg
     1501 aatggcttgg cggcatttcc tgaaggattc gtcgttccgt tcttggcgag cagcatatcc
     1561 accaactgca gctcgttgac cgatttccga tctaccgtct gtaactccgc gcccggagtc
     1621 gactgcggaa ctgccgtacc tgctgcggcc ggcggcgtct ccggtgacga ttttgcctgc
     1681 gccacctcga gttgccggga aggcgcatcg ggtgctgatc gcaacgtgta aaaagcaccg
     1741 ccggcgatcg ccagcgctgt cccggcgatg ccgaccaacc atatagcctt gcccattccc
     1801 ttcgtggccg ccgcagtagc cgccaccgcg gccggtgctc gaggtgaggc ggccggtgac
     1861 gaattgcccg ccggcgtccc tggcgaatcc tgcacggcca acagggcgcg aaactcggca
     1921 acgctgcgcg ggcggtcttc g
//
LOCUS       DAOSIT010000474         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_50930_length_1940_cov_5.949072, whole genome shotgun sequence.
ACCESSION   DAOSIT010000474 DAOSIT010000000
VERSION     DAOSIT010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50930_length_1940_cov_5.949072"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLL14_10100"
     CDS             complement(<1..264)
                     /locus_tag="PLL14_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04322.1"
                     /translation="MLHSPRLKSRGQRLVHAPQGFNTEPRRLVVDPQSLADTAPKLAR
                     PQPRPAGAQVGAARRSAGGGFAASAPAAVATAHRFAQGIERRAL"
     gene            complement(592..861)
                     /locus_tag="PLL14_10105"
     CDS             complement(592..861)
                     /locus_tag="PLL14_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOG04323.1"
                     /translation="MAFEIEFDPEAVKDLKCLDRQIQRRLLGFLKQRVAPLENPRDIG
                     EALAGAKLGRYWKYRVGDWRIICDIQDGRIVVRVLRIGNRREVYR"
     gene            complement(851..1057)
                     /locus_tag="PLL14_10110"
     CDS             complement(851..1057)
                     /locus_tag="PLL14_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin subunit"
                     /protein_id="HOG04324.1"
                     /translation="MLAIRLPETIEARLTALASETGRTKTALAREAIIEYIDDLEDYY
                     LVEARVRLNRPAIPLAEVERELGL"
     gene            complement(1167..>1940)
                     /locus_tag="PLL14_10115"
     CDS             complement(1167..>1940)
                     /locus_tag="PLL14_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HOG04325.1"
                     /translation="RCNLPAAILGGLTYQRHPTPLQIDGVADLHKPLWALLDDIPEAP
                     ERAGRFAGYMQNHFRLHQPEDAGLAAGQASARARATYLRVVRGWAFDAESREAAVLKG
                     WVESRFGLCPRHHGEALREPGSRAWQRYVESRAQGLRGTNALEAQLDLLYAYSQYELA
                     RQFPLRTHLRLYRGVNRLAEHETIHHDPGGDKVLLLNNINSFSRSRERAGEFGDDLLA
                     VDVPLPKLAFFSHLLPGMLRGEDEYVVIGGLYRVRLARF"
BASE COUNT          323 a          622 c          641 g          354 t
ORIGIN      
        1 aagggcgcgg cgctcgatgc cttgcgcgaa tcgatgggca gtcgctacag ccgccggcgc
       61 agaagcagcg aagccgccgc cggctgaacg gcgcgccgcc cccacctgcg cgcccgccgg
      121 tcgcggttgc gggcgcgcca gcttcggcgc cgtgtcggcg aggctttgcg ggtcgacgac
      181 gagtcttcga ggttcagtgt tgaatccctg cggcgcatgc acgagtcttt gacccctgga
      241 cttgagtctt ggggagtgaa gcatgactct ccgcggcttg agctggagtc ttcgcgacgc
      301 atgcacgcgt ctcaagagct tggccctgac tctccgaggc tcgctcttgg ctccccgagg
      361 tttacgcgtg actctttaca gttcgacggt gagtctgcgg gcctcgctaa cgaccatcaa
      421 cggcctaatg tccagtcttc gggaccgacg cgcaagcttg tctgggtgac gaatgcgctt
      481 gctggcagca ggaacgcgca cctgatgtca ggaaatgtcg acatcagcat cgtcaccatg
      541 gggttcatcc tctggctcgc gatgctgctc gtcaactgcg ctgcgcgcga gctatcgata
      601 aacctcgcgc cgattgccga ttcgcaacac acggacaacg atacgaccat cctgaatgtc
      661 gcaaatgatg cgccagtcgc caacgcggta tttccagtag cggcccaact tcgctccggc
      721 gagtgcttcc ccaatatcgc ggggattttc cagtggggcg acacgttgtt tcaggaaccc
      781 aagcagccgc cgttggattt ggcgatccag acatttcaag tctttcacgg cctccggatc
      841 gaactcgatc tcaaaggcca agctcacgct ccacttctgc cagaggaatc gccgggcgat
      901 tcaacctgac ccgtgcctcg accaggtaat agtcttccag atcatcgata tattcgatga
      961 tggcttcgcg cgccagcgcg gtttttgtgc gcccggtttc gctggccagg gcagtgaggc
     1021 gcgcttcaat ggtttcaggc agacggatgg ctagcatggc gacattcctc cgaggagacc
     1081 agtcaagttt agcacgccgg aaaacaggag agatggctac ccttctcgcc ctgtgtgggg
     1141 tgctctgtgt accacccccc cgcgcttcag aagcgcgcca gccgcacccg gtagagaccg
     1201 ccgatgacca cgtattcgtc ttcgccgcgc agcatgccgg gcagcaggtg gctgaagaag
     1261 gcgagcttgg gcagcggcac gtcgacggcc agcaggtcgt cgccgaattc accggcgcgc
     1321 tcgcgcgagc gcgagaagga gttgatgttg ttcagcagca gcaccttgtc gccacccggg
     1381 tcgtggtgga tggtctcgtg ctcggcgagg cgattgacgc cccggtacag ccgcaggtgg
     1441 gtgcgcagcg ggaactgccg cgccagttcg tactggctgt aggcatacag caggtcgagt
     1501 tgcgcctcca gggcgttggt gccgcgcagg ccctgcgcac ggctctccac gtagcgctgc
     1561 caggcgcggc tgcccggttc gcgcagcgct tcgccatgat ggcgcgggca gaggccgaaa
     1621 cgcgactcga cccagccctt caacaccgcc gcctcgcggc tttcggcgtc gaaggcccag
     1681 ccgcgcacga cgcgcaggta ggtggcgcgg gcgcgcgcgc tcgcctggcc ggcagcgagc
     1741 ccggcgtctt ccggctggtg caggcgaaag tggttctgca tgtagccggc gaaacgcccg
     1801 gcgcgctcgg gggcctcggg gatgtcgtcg agcagcgccc acagcggctt gtgcaggtcg
     1861 gcgacgccgt cgatctgcag cggcgtcggg tggcgctggt aggtcaggcc gccgaggatc
     1921 gccgccggca gattgcagcg
//
LOCUS       DAOSIT010000475         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51444_length_1926_cov_11.369321, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000475 DAOSIT010000000
VERSION     DAOSIT010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51444_length_1926_cov_11.369321"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLL14_10120"
     CDS             <1..614
                     /locus_tag="PLL14_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOG04326.1"
                     /translation="ALQMDIKIQGITKEIMQVALAQAGEARLHILKQMQDSMSGHREE
                     VSTYAPRLYTMKINPEKIRDVIGKGGAVIRAITEETGTTIDIKDDGTITIASVSGDAA
                     DAARARIESITADVEVGKVYDGTVLKLLDFGAIVSILPGRDGLLHISQIANERVNAVA
                     DYLKEGQKVRVKVLEADEKGRVRLSMKALTAEEGGVGAAAPAS"
     gene            complement(708..1133)
                     /gene="dksA"
                     /locus_tag="PLL14_10125"
     CDS             complement(708..1133)
                     /gene="dksA"
                     /locus_tag="PLL14_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOG04327.1"
                     /translation="MAEELLHKHFTPYVPEPGEEFMNSDQLAHFRGILEALKRQLMED
                     IERTVHTMKDEATVFADPNDRASQETDIAIELRNRDRERKLIKKIEETIARIDSGDYG
                     YCAACGVEIGIKRLEARPTATLCIDCKTLEEVREKQIAK"
     gene            complement(1268..>1926)
                     /locus_tag="PLL14_10130"
     CDS             complement(1268..>1926)
                     /locus_tag="PLL14_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG04328.1"
                     /translation="EPGMVDQMRAMASRLLDLGSATVLAGRAMETAGKFSRALQDGHP
                     LDAASEVVRAVLPGVAEPAWARTGAAIRAMRESAGLTIAEVGAAIDLKDPSLIEALEN
                     GRIALSFELILRLAAVFGRNDPVGFVMRFTRTANPDLWKSLEGLGVGKLVLQTVREHQ
                     FVNVYRSNDDARTLSDAEFAEILSFTQAAFETAMALRARYLARGDGPPVEGDPAGAGR
                     "
BASE COUNT          381 a          631 c          570 g          344 t
ORIGIN      
        1 ccgcgctgca gatggacatc aagatccagg gcatcaccaa ggagatcatg caggtcgccc
       61 tggcgcaggc cggtgaagca cgcctgcaca ttctcaagca gatgcaggac tcgatgtccg
      121 gacaccgcga ggaagtctcg acctacgccc cgcgcctgta caccatgaag atcaaccccg
      181 agaagatccg cgacgtgatc ggcaagggcg gtgcggtgat ccgggcgatc accgaggaaa
      241 ccggaacgac catcgacatc aaggacgatg gcacgatcac catcgcctcg gtcagcggtg
      301 acgcggccga tgccgcgcgg gcaagaatcg agtcgatcac cgccgatgtt gaagtcggca
      361 aggtctatga tggcaccgtg ctcaagttgc tcgacttcgg cgccatcgtc agcatcctgc
      421 ctggacgaga cggcctgctg catatttcgc agattgccaa cgaacgcgtc aatgccgtcg
      481 ccgattatct caaggaaggc cagaaagtgc gcgtcaaggt actggaagcc gacgagaagg
      541 gtcgcgtgcg cctgtcgatg aaagccctga cggccgaaga aggtggtgtc ggggcggcag
      601 cgccggcgtc gtaaccacgc aacagcagat caaaaaagga cgccacggcg tccttttctg
      661 tttccgcgcg cggcaaattc gtccgcgctg cacacgtctg ggaccgctta tttggcgatc
      721 tgcttctcgc gcacctcttc cagcgtcttg cagtcgatgc acagcgtcgc cgtcggccgc
      781 gcttccagac gcttgatgcc gatctcgacg ccgcaggccg cacagtagcc atagtcgcca
      841 ctgtcgatac gcgcgatggt ttcctcgatc ttcttgatca gcttgcgctc gcggtcacgg
      901 ttgcgcaact cgatggcgat gtcggtttcc tggctcgcac gatcattggg atcagcaaaa
      961 accgtcgctt catccttcat cgtgtgcacc gtgcgctcga tgtcttccat caactgccgc
     1021 ttgagggctt cgaggatgcc gcgaaaatga gccagctgat cgctgttcat gaactcctcg
     1081 cccggttccg gtacgtaggg agtgaaatgc ttgtggagca attcttcagc catgttcgaa
     1141 gaccctgttt tctgtttgca aaaaaagacg actagatatc agaaatagca gcgtctggca
     1201 agcaacggtc tgctgatcgg atgcgtgcgg gcggcggcaa ccggctgcca gggcgcgccg
     1261 ggaacgttca tctgcccgcc cccgctggat cgccttcgac aggagggccg tcaccgcggg
     1321 ccagatagcg tgcgcgcagc gccatcgccg tctcgaatgc cgcctgcgtg aaactcagga
     1381 tctccgcgaa ctccgcgtcg ctgagcgtcc gggcatcgtc gttgctgcga tagacgttga
     1441 cgaactggtg ctcgcgcacc gtctgcagca ccagcttgcc gacccccaag ccttcgaggc
     1501 tcttccacag atcgggattc gccgtgcgcg taaagcgcat cacaaaaccg accgggtcat
     1561 tgcgcccaaa gaccgccgcc aaacgcagga tcaactcgaa cgacaaggca atgcggccat
     1621 tctcgagcgc ctcgatcagc gagggatcct tgagatcgat cgccgcgccg acctcggcaa
     1681 tcgtcagtcc cgccgactcg cgcatggcgc ggatcgccgc cccggtcctg gcccatgcgg
     1741 gctcggcgac gcccggcaac acggcccgca ccacttcgct ggccgcgtct aacggatgcc
     1801 catcctgcag cgcccgcgag aacttgcctg cagtctccat ggctcgcccg gcaagcaccg
     1861 ttgccgaccc caggtccagc aaacggctgg ccatcgcgcg catctgatcg accatgcccg
     1921 gctcgg
//
LOCUS       DAOSIT010000476         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51445_length_1926_cov_8.740246, whole genome shotgun sequence.
ACCESSION   DAOSIT010000476 DAOSIT010000000
VERSION     DAOSIT010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51445_length_1926_cov_8.740246"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLL14_10135"
     CDS             <1..442
                     /locus_tag="PLL14_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04329.1"
                     /translation="AILLGLAILGTVGYQLAPLLNPKTDIALPLSSCDLGQGACAATL
                     PNGTRIEFAIEPRPIPVLRPLKLQVTLTGGEVRRIEVDFTGTDMKMGYNRPQLERQEG
                     SSDHFVGMASLPVCITGSMEWEATLLIDTGKAIVAVPFRFLSGK"
     gene            704..1294
                     /locus_tag="PLL14_10140"
     CDS             704..1294
                     /locus_tag="PLL14_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG04330.1"
                     /translation="MHRTIFDTPVVSSLLRACSVAFLHLTGWTVEGRLPTGTAKCVLI
                     AAPHTSNWDLPYTLMVAFSLRLHIYWMGKQSLFNPPFRGLMMWLGGIPVNREKSTGFV
                     LASVAAIKAATDGLQLVVPPEGTRGRTRHWKTGFYHIALGAEVPIVMAYMDYGRKVSG
                     LGPVFTPTGDIERDMASIKSFYAPFKGRNARLFDPE"
     gene            1337..>1926
                     /locus_tag="PLL14_10145"
     CDS             1337..>1926
                     /locus_tag="PLL14_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOG04331.1"
                     /translation="MMIERMRWLLILILLSAASGASLAATGSDQPANLDLHPDATVAT
                     AAVEVDGRVLFRVRGFSSFPARERAAAITERIKRLAGDPAVPAGSVRLVESDGYSSIM
                     AGDRRIMVLFDSDARIEEVARETLGLALCERIGKAIDEYRHDRDTQVLQRRGLEAAGA
                     TAALATMVTLLVCLARRLNGLLERRFRSRIHHLGIQS"
BASE COUNT          351 a          630 c          597 g          348 t
ORIGIN      
        1 cgccatcctg ctgggactcg ccatcctcgg caccgtcggc taccaactgg cgccgttgct
       61 caacccgaaa accgacattg ccctgccgct gtcgagttgt gacctcggtc agggagcctg
      121 cgcggcgaca ctccccaacg ggactcgcat cgagttcgcg atcgaaccac ggccgattcc
      181 ggtactccga ccgctgaagc tgcaagtcac tctcacgggt ggcgaggtac gcaggatcga
      241 ggtcgatttc accggcacgg acatgaaaat gggttacaac cgaccgcaac tggaaaggca
      301 ggaaggcagc agcgatcatt tcgtggggat ggcgagcctg ccggtatgca tcaccggaag
      361 catggaatgg gaagcgacgc tgctgatcga taccggcaaa gcgatagtcg ctgtgccgtt
      421 ccgcttcctc agcggcaagt aaaaccccac ccacgttacc gacctcgctt gccggagctc
      481 gtgtccatcg gcaagcccgg agatgattcc tccgcctctg ccttgccgat gatgcgcaat
      541 gggtttacat ttgcggcaac aatccattga ccgggaacca gggtggcgac gcccgaccca
      601 cgcaaccgca gcaatacctc gagtcctgct ccattgccgg ccacgcgctc tgcgcggcgc
      661 gcagcggctc ggcaaccgac tcgtcgcctg ggacactgcc cgcatgcacc gcacgatttt
      721 cgataccccg gtggtaagct cactgttgcg cgcctgctcc gtggcctttc tgcacttgac
      781 cggctggact gtcgaaggcc gtctgccgac tggcacagcc aaatgcgtac tgatcgccgc
      841 accgcatacc agcaactggg acctgcccta cacgctgatg gtggccttca gcttgcgcct
      901 gcacatctac tggatgggca agcagagcct cttcaatcca ccctttcgcg gtctgatgat
      961 gtggctgggc ggcatcccgg tgaaccgcga gaagtcgacc ggcttcgtgc tcgcgtcggt
     1021 ggcggcgatc aaggccgcca ccgacggact acagttggtc gtccccccgg agggcacacg
     1081 cggccgtacg cgccactgga aaaccggttt ctaccacatt gccctcggcg ccgaagtgcc
     1141 gatcgtcatg gcctacatgg actacggccg caaagtgagc ggcctgggtc cggtattcac
     1201 gcccaccggc gatatcgaac gcgacatggc atcgatcaag tcgttctacg ctcccttcaa
     1261 gggtcgtaac gccaggctgt tcgatcccga ataggccagc gtgatcgtgg gcaggcggtt
     1321 tgaggagcgt tgaagaatga tgattgagcg tatgcggtgg ttgctgatcc tgattctgct
     1381 cagcgccgcc agcggcgctt cgcttgccgc caccggcagc gatcaacccg cgaatctgga
     1441 tctccatccc gacgccacag tcgcgacggc agcggtcgaa gtcgacggcc gcgtactgtt
     1501 ccgcgtgcgc ggcttcagca gtttccccgc acgcgagcgt gcggcggcga tcaccgagcg
     1561 gatcaagcga ctggcgggcg atccggcggt gccggcgggt tcggtgcgac tggtcgagtc
     1621 cgacggttat tcctcgatca tggccggcga ccgcaggatc atggtgctct tcgattcgga
     1681 cgcgcgcatc gaagaagtcg cacgcgaaac ccttggactg gccttgtgcg agcggatcgg
     1741 caaggccatc gacgaatacc gccacgaccg ggacacgcag gtattgcagc gacgcggcct
     1801 ggaggctgcg ggtgccacgg cggctctggc cacgatggtg acgctgctgg tgtgcttggc
     1861 acgccggctc aatggcctgc tggagcggcg cttccggagc cgaatccatc acctcggcat
     1921 ccagtc
//
LOCUS       DAOSIT010000477         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51477_length_1925_cov_8.223529, whole genome shotgun sequence.
ACCESSION   DAOSIT010000477 DAOSIT010000000
VERSION     DAOSIT010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51477_length_1925_cov_8.223529"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="dapA"
                     /locus_tag="PLL14_10150"
     CDS             <1..735
                     /gene="dapA"
                     /locus_tag="PLL14_10150"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG04332.1"
                     /translation="PTVNLQEHCELIRVAVDQAAGRIPVIAGTGANSTAEAIELTGFA
                     RRAGASAALSVVPYYNKPTQEGLYRHFRAIAEAVDIPVILYNVPGRTVADMSNDTALR
                     LAEIPNIAGIKDATGNMDRGIELIERAPDGFAVYSGDDASACALILMGARGDISVVAN
                     VVPRLMHEMCAAALAGDARRARELNARLLGLHRQMFCEANPIPVKWACRELGLIEGGL
                     RLPLTPLSPDCHARVRAAMVQAGALV"
     gene            751..1893
                     /gene="bamC"
                     /locus_tag="PLL14_10155"
     CDS             751..1893
                     /gene="bamC"
                     /locus_tag="PLL14_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOG04333.1"
                     /translation="MSPPASFSRIGILLVMLTACNGSLLEPKRIEYKTAGSGSAVPTL
                     EVPPDLTSPTRDDRYAVPDIGGKGTATFSAYTAERSPQARAQQNSEVLPEVEQARIER
                     AGTQRWLVATGSPDKLWGPVKEFWQETGFLIKLELPDAGVMETDWAENRAKIQQDFIR
                     NLLGKVIDSVYSTAERDKFRTRLERGSTPGTTDIFISHRGMYEIYVSEGKDQTKWQPR
                     PADPELEAEMLRRLMIRLGSEEKRANAALQAVQEKPVERARLSRGSDGAGTLEVEESF
                     DRAWRRVGLALDRVGFTVEDRDRSRGLYFVRYVDPESDNEKKDDGFFSRLNPFKGSAA
                     KAQTQFRIFVGEAGSRSTVQVLSLEGGVDQSETSKKILNLLYDQLK"
BASE COUNT          373 a          577 c          627 g          348 t
ORIGIN      
        1 ccgacggtta acctgcagga acattgcgaa ctgatccggg tcgcggtcga tcaggccgcc
       61 ggccgcattc cggtgattgc cggtacgggc gccaactcga cggctgaagc gatcgagttg
      121 accgggttcg cgaggcgagc gggcgccagc gcggccttgt cggtcgtgcc gtactacaac
      181 aagccgacgc aggagggtct ctatcgccac ttccgcgcca ttgcggaagc ggtggacatc
      241 cccgtcatcc tctacaacgt gccgggtcgg acggtcgccg atatgagcaa tgacaccgcc
      301 ttgcgcctgg cggaaattcc gaacattgcc gggatcaagg acgccaccgg caacatggat
      361 cgagggatcg aactgatcga gcgcgcgcct gatggttttg ccgtctacag cggtgatgat
      421 gccagtgctt gtgcattgat cctgatgggc gcgcgtggcg atatctcggt ggtggccaac
      481 gtcgtgcccc gcttgatgca cgaaatgtgc gccgcagcgc tggctggcga tgcgcgtcgc
      541 gcacgggaat tgaatgcgcg cctgctcggc ctgcatcgac agatgttctg cgaggcgaat
      601 ccgattccgg tcaaatgggc gtgccgggaa ctcgggctga tcgaaggcgg gctgcgactg
      661 ccgctgacac ccttgtcgcc ggattgtcac gcgcgtgttc gtgccgccat ggtccaggcc
      721 ggcgcacttg tctagaatca gggtcgtcaa atgagtcctc ccgcttcgtt ctcccgaatt
      781 ggcattctgc tggtcatgct cactgcctgt aacggatccc tgctcgaacc caagagaatc
      841 gagtacaaga ccgcggggag cggcagcgcc gtgccgacgc tggaggtgcc gcctgacctg
      901 acctcaccca cccgcgatga ccgttatgcc gttcccgata tcgggggcaa ggggactgcc
      961 accttctcgg cctacaccgc tgaacgttct ccgcaggcca gagcgcagca gaacagcgaa
     1021 gtcctcccgg aagtcgagca ggcgcgcatc gagcgggcgg gcacccagcg ctggctggtg
     1081 gccacaggtt cgccggacaa gctctggggt ccggtcaagg agttctggca ggaaaccggc
     1141 ttcctgatca agctcgaact gcctgacgcc ggggtcatgg aaaccgattg ggccgagaac
     1201 cgcgccaaga tacaacagga cttcatccgc aacctgttgg ggaaggtgat cgactcggtc
     1261 tattcaaccg ccgagcgcga caagttccgt actcgcctgg agcggggaag cacgccgggg
     1321 accaccgaca tcttcatcag ccaccgcgga atgtacgaaa tttacgtttc cgaaggcaag
     1381 gatcaaacca agtggcaacc gcgtccagcg gacccagaac tggaagcgga gatgctgcgc
     1441 cgcttgatga ttcgtctggg tagcgaagag aagcgggcca atgcggctct gcaagcggtc
     1501 caggagaagc ccgtcgagcg ggccaggctc tcgcggggca gcgacggcgc cggtacgctc
     1561 gaggtggagg aatcgttcga tcgtgcctgg cgtcgagtcg gtctggcgct ggaccgtgtg
     1621 ggtttcaccg tggaagaccg ggatcgttcc agaggtctgt acttcgttcg ttacgtcgac
     1681 cccgaaagcg acaatgagaa gaaggacgat ggcttctttt cccgcctgaa tcccttcaag
     1741 ggaagcgccg ccaaggcgca aacgcaattc cgcatcttcg ttggcgaggc gggcagccgg
     1801 tcgaccgtgc aggtgctctc gctggagggt ggcgttgatc agtccgaaac ctcgaagaag
     1861 attctcaacc tgctctacga ccagttgaag tgatccgttt cgcatcgcta ggcagcggca
     1921 gcaag
//
LOCUS       DAOSIT010000478         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51601_length_1922_cov_8.159079, whole genome shotgun sequence.
ACCESSION   DAOSIT010000478 DAOSIT010000000
VERSION     DAOSIT010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51601_length_1922_cov_8.159079"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..977)
                     /locus_tag="PLL14_10160"
     CDS             complement(6..977)
                     /locus_tag="PLL14_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906738.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG04334.1"
                     /translation="MRRPFAEFRRHVSSRAFFYQLLVAGLALALVWLLVEQTGAELRT
                     RGIRSGFAFLFEPAGFSIGESLLDFDAEQPYWRAFLVGVLNTLRVAVAAIVLATVLGL
                     CLGACSLTRNVLLRGLCRAYVEVFRNIPLLLQLLTWYFLLAEKLPAADAALNPLPGVF
                     LSKSGLAFPWGGEVPAAGDFGIAGGGAVTPEFLALLLGLSCYGAAYLAEIVRAGILSV
                     PRGQVDAAAALGMTPIQRLRAVVLPQALRLMIPPATNQFLNLTKNSSLAVAVGYPDLV
                     SIANTSLNQTGQAAECISILMAVYLALSLLTAAATGWYNRRQLRRGH"
     gene            complement(958..>1922)
                     /locus_tag="PLL14_10165"
     CDS             complement(958..>1922)
                     /locus_tag="PLL14_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG04335.1"
                     /translation="AHAGKTLDSVRQQGRVACGVSTGLAGFSGVDSKGNWSGLDTDIC
                     RSIAAAVLGDPGKVKWLPLNSQQRFTALQAGEVDVLSRNTTWTLTRDASMGLHFTAIT
                     YYDGQGFMVPKQSKASSVKQLRNAEICVQSGTTTEKNLNDYFRAQGAKVRPVVFESFE
                     ASLKAFFSGRCQAYTTDVSSLVSIRDKEAPKPGDYVILPEVISKEPLGPAVRRGDDEW
                     FAIVKWLVFALIEAEELGITQANADSLRNSSNPAVQRLLGSGEDSGRLLGLDREWALR
                     AIKAVGNYGEIFERNLGSGSSLKLPRGSNALWTKGGLMYAPPVR"
BASE COUNT          362 a          632 c          623 g          305 t
ORIGIN      
        1 ggccatcagt gtcccctgcg caactgccgc cggttatacc agccggtcgc cgcggcggtc
       61 agcaacgaca atgcgagata gaccgccatc aggatgctga tgcactcggc ggcctgcccg
      121 gtctggttga ggctggtatt ggcaatcgat accaggtctg gataacccac ggcgacggcg
      181 agcgaggagt tcttggtcag gttgagaaac tggttggtcg ccggtggaat catcagacgc
      241 agggcctgcg gcaggaccac cgcgcgcagg cgctgtatcg gcgtcatgcc gagtgctgcg
      301 gccgcatcga cctggccgcg cggcaccgaa aggatgccgg cacggacaat ttcagccagg
      361 taggccgcgc catagcaact caggccgagc agcagggcaa gaaactccgg cgtcacggcg
      421 ccaccgccgg caatgccgaa atcgcccgcg gccggcactt ccccacccca gggaaaggcg
      481 agcccgctct tgctcaggaa aacccccggc agcgggttca gcgcagcgtc cgccgctggc
      541 agtttctcgg ccagcaggaa ataccaggtg agcagttgca gcagcaaggg gatgttgcgg
      601 aagacttcga cgtaggctcg gcacagtccg cgcagcagaa cattcctcgt caggctgcag
      661 gcgccgaggc acaggccaag gacggtcgcc aggacgatgg ccgccaccgc cacccgcaag
      721 gtgttgagta cgccgacgag aaaggcacgc caatacggtt gctcggcatc gaagtcgagc
      781 aggctctcgc cgatcgagaa gccggccggc tcgaagagaa aggcgaaacc gctgcgtatg
      841 ccacgcgtcc gcagttcggc gccggtctgc tcgaccaaca gccagacgag ggccagcgcc
      901 agcccggcga ccagtagctg atagaagaaa gcgcgcgatg aaacgtggcg ccgaaactca
      961 gcgaacgggc ggcgcataca tcaggccacc cttggtccac agcgcattcg acccacgcgg
     1021 cagcttgagc gacgaaccgg atccgagatt gcgctcgaaa atctcgccgt agttgcccac
     1081 cgccttgatc gcccgcagcg cccactcgcg atcgaggccg agcaggcggc cgctatcttc
     1141 gccgctaccg agcagccgct gcacggcggg gttggaactg ttgcgcaagc tgtcggcgtt
     1201 ggcctgcgtg attcccagtt cctcggcttc gatgagcgcg aagaccagcc acttgacgat
     1261 cgcgaaccac tcgtcgtcgc cgcgccgcac tgccgggccc agcggttcct tggagatcac
     1321 ttcgggcagg atcacatagt cgccgggctt cggcgcctcc ttgtcgcgaa tcgacaccag
     1381 gctggacaca tcggtcgtgt aggcctggca acgcccggag aagaacgcct tgagggaggc
     1441 ttcgaagctt tcgaaaacca ccggcctgac cttcgccccc tgcgcgcgga agtagtcgtt
     1501 caggttcttt tcggtggtgg tgccggactg gacgcagatt tcggcgttcc tgagctgctt
     1561 gacgctgctc gctttcgact gcttcgggac catgaacccc tggccatcgt agtaggtgat
     1621 ggcggtgaaa tgcaggccca tcgaagcgtc gcgggtcagg gtccaggtgg tgttgcgcga
     1681 cagcacatcc acctcacccg cctgcagggc ggtgaagcgc tgctgtgaat tgagcggcag
     1741 ccatttcacc ttgcccgggt ctcccagcac cgccgccgcg atgctgcggc agatgtcggt
     1801 atcgagcccg ctccagtttc ctttgctgtc caccccggaa aacccggcaa ggccggtgct
     1861 caccccgcag gcgacgcggc cctgctggcg gaccgaatcc agggtcttgc cggcgtgcgc
     1921 cg
//
LOCUS       DAOSIT010000479         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51678_length_1920_cov_8.046649, whole genome shotgun sequence.
ACCESSION   DAOSIT010000479 DAOSIT010000000
VERSION     DAOSIT010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51678_length_1920_cov_8.046649"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLL14_10170"
     CDS             complement(<1..1180)
                     /locus_tag="PLL14_10170"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOG04336.1"
                     /translation="MRVLVVAAACLGVAHAQHLPAPSDRPDSPGKSLPTATAAATANR
                     ITPSGGQGRRVALVIGNGEYRYPDNLPRLANPVHDAEDIASALRRFGFEVIERKNQTL
                     EAMHQSIAEFGSRIGGSEAALFFFAGHGIQVRNQNYLMPINAKVESEAMVPYQGVNVN
                     QILDEMDNGRSSANIVILDACRNNPVTGKFRSGQTRGLASPGGVPKGTVIVYATDPGN
                     VAADGDGRNGLLTAGLLAAFKGRDLSLDGVLTVASAEVERASAQAQTPYVNGPKTLQK
                     NFHFRVTVDPGRSEVERTFWTSIERSTDAADFEAYLKNYPQGSYRDLAENQLRRLRAK
                     PVEATRIPAPAPAPAAVPAATVAIERKGAGQNSSEGRQTGTAQLPSRVEKPALQIGDS
                     W"
     gene            1431..1625
                     /locus_tag="PLL14_10175"
     CDS             1431..1625
                     /locus_tag="PLL14_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HOG04337.1"
                     /translation="MDVSGIASVATEMSQARTVDAVQIAVLKKALDIQAQGAMQLVEA
                     AAQVIPNNPPNLGNHIDTSA"
BASE COUNT          346 a          585 c          607 g          382 t
ORIGIN      
        1 cccaactgtc gccgatctgg agagcgggtt tctcgacacg ggacggaagt tgcgctgtgc
       61 ctgtctggcg tccttcgctg ctgttctgtc cggcgccttt tctttcaatt gccaccgtag
      121 ccgccggaac ggcagcgggg gcgggggcgg gggcgggaat tctggttgct tcgaccggtt
      181 ttgcccgcaa gcgccggagc tggttttccg ccaaatcccg gtagctgcct tgcgggtaat
      241 ttttcagata cgcctcgaaa tcggccgcat ccgtactgcg ctcaatgctg gtccagaaag
      301 tcctctccac ttcgctgcgg ccggggtcca cggtgacgcg gaagtgaaag ttcttctgca
      361 gtgtcttggg gccgttgacg taaggggtct gcgcttgcgc gctggcgcgc tcgacttcag
      421 cactcgcaac cgtcagtacc ccatcaagac tcaggtcccg gcccttgaac gcagccagca
      481 ggcccgcggt caacaagccg ttgcgaccgt cgccatcggc ggcgacattg cccgggtcgg
      541 tggcatacac gatgaccgtc cccttgggta caccgccggg actggcgagg ccacgcgttt
      601 ggccggatcg gaatttgccg gtgaccggat tgttgcggca ggcatcgagg atgacgatgt
      661 tggcgctgct cctgccgtta tccatctcgt cgaggatctg gttgacgttg actccctgat
      721 agggaaccat cgcttcgctt tccactttgg cgttgatcgg catcaggtag ttctggtttc
      781 tcacctgaat gccgtgcccg gcaaagaaga acagcgcggc ttcgctgcca ccgatccggc
      841 tgccaaattc ggcgatgctc tggtgcatgg cttccagcgt ctggttcttg cgctcgatga
      901 cttcaaaacc aaagcgtcgc aaggcgctgg caatgtcttc cgcgtcgtgg accgggttgg
      961 ccaacctggg caggttgtcc gggtagcggt actccccgtt gccgatcacc agagcgacgc
     1021 gtcgtccctg accgccgctg ggggtgatgc gattcgctgt cgcggccgcc gttgcggtgg
     1081 gcagcgactt gcccggcgaa tcgggacgat cggatggtgc tggcagatgc tgcgcgtggg
     1141 ccacgccaag acacgctgcc gcgacgacca ggacgcgcaa aatgcaggta catacggcca
     1201 ataacaatcg ggatggcatg attggcgatc ttatgctcgc gaagagggct gcacaatagg
     1261 gacgcggagg tttggcgcct tgcgtagccc gacgtacctg tggcaccgcg ccttccggaa
     1321 agcgcatccc ggcagcaaac ccgaagatcc tgctgaggcc ggcactcaag aatctgtccg
     1381 aactgccgta aagatttttt catccgtaga aaacccgagg agcagcgtgc atggacgtga
     1441 gtggaattgc ctcagtggcg acagaaatga gccaggccag aaccgttgat gcagtgcaga
     1501 tcgcggtact caaaaaagca ctggatatcc aggcgcaggg cgccatgcaa cttgtcgaag
     1561 cggcggcaca ggtcatcccg aacaatccgc cgaacctggg caaccatatc gacacctcgg
     1621 cgtagctttg ggcctgaccc aggcctggtg tcgctgccgg ctacggatca actaccggcg
     1681 ccgagtgtcc agggtaaccg ccacccttcg gcgatcgcct tgccgacgtc ggtggtgctg
     1741 gccttgccgc ccatgtccgg cgttcgcggc caggtacaaa caggcaaggg ccaccgtcgc
     1801 ccgatggccc tagtattgcg aagcttgctt ccgatcagcc gatgttgaac atcagggcgt
     1861 cagcgcccga gttcagcgcg acactgatcg gcaactggtg ggtcgtcgga ccccagaact
//
LOCUS       DAOSIT010000480         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51771_length_1918_cov_9.361782, whole genome shotgun sequence.
ACCESSION   DAOSIT010000480 DAOSIT010000000
VERSION     DAOSIT010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51771_length_1918_cov_9.361782"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1007
                     /locus_tag="PLL14_10180"
     CDS             72..1007
                     /locus_tag="PLL14_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOG04338.1"
                     /translation="MSTLICGSIAFDNIMVFHDRFKNHILPEQIHILNVAFLVPEMRR
                     EFGGCAGNIAYNLKLLDGDPLIMATVGDDADPYLVRLDDLGLSRAHVRRIAGSFTAQA
                     FITTDLDDNQITAFHPGAMTYSHLNRVTDVRDATLGIVAPDGRDGMLQHAQDLAAAGV
                     PFVFDPGQGLPMFSGEELLGFMRLANYACFNDYEAKLLCDRTGRSLEQLATEVQALIV
                     TLGGAGSRILADGRCHEIPSVQAEAVVDPTGCGDAYRSGLLYGIAAGWDWEKVGRLAA
                     VMGAIKIAHRGGQNHRPGRADISDLFAKAFGTNPW"
     gene            1019..1471
                     /locus_tag="PLL14_10185"
     CDS             1019..1471
                     /locus_tag="PLL14_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOG04339.1"
                     /translation="MNLLRMFFLMLVSVTLVACVSSQSASVYSPQQARQEMTVRQGTV
                     ESVREVIMQGSKSGVGTLAGAAVGGVAGSNIGGGKGQIVGAILGAVAGGLAGSAIEEG
                     ATRKVALEVTVRLNGGQLVAVVQETDERFLPGERVRLLSRGGDTRVTH"
     gene            complement(1479..>1918)
                     /locus_tag="PLL14_10190"
     CDS             complement(1479..>1918)
                     /locus_tag="PLL14_10190"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOG04340.1"
                     /translation="PWEHAVILNRLIAMSVLAGVLTACATSESLPARSTTDARQERGK
                     NSKEQKQLKMQLASGVYRCEFGQSIEIQRVTGNLNLIELRWQGHRHTLERQRSASGLP
                     RYEDPQNGLLWIDLPWKSVLMDASSGRPLANECKVAQRTTTGS"
BASE COUNT          342 a          560 c          586 g          430 t
ORIGIN      
        1 ggcgaccggt gcatgtcgcc atcaatgcta tacttttcct caaagtagtc ctctagacct
       61 tcgggatgtc catgtccact cttatttgcg gctcaatcgc cttcgacaac atcatggttt
      121 tccatgatcg tttcaagaat cacatcctgc ccgagcagat tcacattctc aacgtggctt
      181 tcctggtacc cgaaatgcgg cgtgagtttg gcggttgcgc gggcaacatc gcctacaacc
      241 tgaaacttct cgatggcgac ccgctgatca tggcaacggt tggtgatgat gccgatccct
      301 atctcgtacg cctggacgac ctcggactgt cgcgcgcgca tgtgcgccgg attgccggca
      361 gctttacggc gcaggcgttc atcaccaccg atctcgacga caatcagatc accgcctttc
      421 acccgggtgc catgacctac tcccacctca atcgcgtgac cgatgtgcgg gatgctacgc
      481 ttgggattgt tgctcctgac gggcgtgacg gcatgctcca gcacgcgcag gacctcgcgg
      541 ccgcgggggt gcccttcgtc ttcgaccccg gacaaggcct gccgatgttc tcgggcgagg
      601 aactgctggg tttcatgcgt ctggccaact acgcctgttt caatgactat gaggcgaagt
      661 tgctgtgtga tcgaacgggc cgatcacttg agcaacttgc gaccgaggtg caagcgctga
      721 tcgtcaccct tgggggcgca ggctcgcgca ttcttgccga cggtcgttgt catgagattc
      781 ccagcgttca ggccgaggcc gtcgtcgatc ccacgggttg tggtgacgcc taccgctcgg
      841 gtctgctgta cggtattgcc gccggctggg actgggagaa ggtgggtcgc ctggcggcgg
      901 tgatgggggc gatcaagatc gctcatcgtg gtggtcagaa ccatcgtcca ggtcgcgctg
      961 acatttctga cctgtttgcc aaggcttttg gtaccaatcc ctggtaaagg atgctgcaat
     1021 gaatctcctc cgaatgtttt tcctgatgct ggtcagcgtc actcttgtcg cctgcgtgag
     1081 cagccagtcg gccagtgtct actcgcccca acaggcgaga caggagatga ctgtccgcca
     1141 gggcaccgtc gagagcgttc gggaggtcat catgcaaggc agcaagtcag gtgtcggcac
     1201 gctggcaggt gccgcagtgg gtggggtggc ggggagcaat attggcggtg gcaaagggca
     1261 gatcgtaggc gcgattctcg gcgcggtggc tggtggcttg gccggttcgg caatcgagga
     1321 aggtgcgacc aggaaagttg cgctcgaagt cacggtcagg ttgaatggtg gccaactggt
     1381 ggccgtcgtc caggaaactg acgaacgctt ccttcccggc gaaagagtgc gtcttctttc
     1441 ccgcggtggc gatacccgcg tcacgcatta gccggcagtc agctaccggt agtcgtcctc
     1501 tgcgccactt tgcactcatt ggcgagcggt cgcccgctgc tggcgtccat caggacgctt
     1561 ttccagggca ggtcaatcca caacaatccg ttctgtgggt cctcgtagcg ggggagaccg
     1621 gaggcagacc tttggcgctc cagtgtgtga cgatggccct gccaacgcag ttcgatcaga
     1681 ttgaggttac cggtaacccg ctgaatctcg atgctctggc cgaactcaca acgataaaca
     1741 ccactggcga gctgcatttt cagttgcttc tgttccttgc tgttcttgcc ccgctcctgg
     1801 cgagcgtcgg tagttgaccg tgcaggcagc gactcgctag ttgcgcaggc cgtcagcact
     1861 ccagccagga cactcatggc gatcaagcga ttcaggatca ccgcatgttc ccaaggtt
//
LOCUS       DAOSIT010000481         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_51972_length_1913_cov_6.491389, whole genome shotgun sequence.
ACCESSION   DAOSIT010000481 DAOSIT010000000
VERSION     DAOSIT010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51972_length_1913_cov_6.491389"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLL14_10195"
     CDS             <1..654
                     /locus_tag="PLL14_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-ACP transacylase"
                     /protein_id="HOG04341.1"
                     /translation="GADFILTGSINQCTVEAGTSDLVKDLLQEMQVQDTDYAPAGDMF
                     EIGARVQVLKRGLFFPVRANKLYELYRSVDSIEQIDQGIARMIQEKYFQRSFAEVYEE
                     CRRHHDAAQIAEADQHPKKKMALIFRWYFGHANRLALQGDAGHKVDFQIHCGPALGGF
                     NQWVKGTPLENWRRRHADEIGLKLMDDTARLLMRRFGALFEQDAAPPIDRRVADCVE"
     gene            666..929
                     /locus_tag="PLL14_10200"
     CDS             666..929
                     /locus_tag="PLL14_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG04342.1"
                     /translation="MEEKIRRFIEETFMFEFGEEITTETDLFKAGVIDSFGYMQLMQF
                     LDSEFGVRFADEELLSNILVSYDHLIASVRARVATQGVREGAA"
     gene            907..>1913
                     /gene="asnB"
                     /locus_tag="PLL14_10205"
     CDS             907..>1913
                     /gene="asnB"
                     /locus_tag="PLL14_10205"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOG04343.1"
                     /translation="MSAKAQHKRDIAQEYGHRGELMCGISGFYGAPPNGVEPAEAVKK
                     MLALIQHRGPDEAGYYVDDRLAMGTVRLSIIDLASGTQPLSDADERHWICYNGELYNY
                     RELRRELEGHGCRFRTQSDTEVVLQAWLFWGEQCLPRLNGAFAFAIYDNVAGSLLLAR
                     DRYGKRPLFHAQHAGALIFASEMKAFLAFRDFQFAADPQQLSSIFAQWTPLPAQSGFK
                     DIHQLPMACFLRVQEGQRSEGCYERLDFARGPAVASEAQAVERVRQALTDSVRLRLRS
                     DVEVGVYLSGGLDSSIITQLVTQMSPHTIHTFSVAFEDKYFDESEDQSEVSRCLGTEH
                     SA"
BASE COUNT          382 a          536 c          619 g          376 t
ORIGIN      
        1 ggtgccgact tcatcctcac cggctcgatc aaccagtgta cggtcgaagc gggtaccagc
       61 gatctggtca aggacctgct gcaggaaatg caggtgcagg acaccgatta cgcgccggcc
      121 ggcgacatgt tcgagatcgg cgccagggta caggtgctca aacgcggcct gttcttcccg
      181 gtgcgcgcca acaagctcta cgagttgtac cggtcggtcg attcgatcga gcagatcgat
      241 cagggaatcg ccaggatgat ccaggaaaaa tatttccagc gttcctttgc cgaggtctat
      301 gaggaatgtc ggcgccacca tgacgccgcc cagattgccg aggcggatca gcatccgaaa
      361 aagaagatgg cactgatctt ccgctggtat tttggccatg ccaatcgcct ggcgttgcag
      421 ggcgacgccg gccataaggt cgattttcag atccattgcg ggccggcact gggaggcttc
      481 aaccaatggg tcaagggcac gccactggag aactggcgcc ggcggcatgc cgacgagatc
      541 ggcttgaaac tgatggatga caccgcgcgc ctgctgatgc ggcgctttgg cgcgctgttc
      601 gagcaggatg ccgcgccgcc gatcgaccgg cgcgtggccg attgcgtgga atgacgagga
      661 agacgatgga agagaaaatc cggcggttta tcgaagaaac cttcatgttc gagttcgggg
      721 aagagatcac gacggagacc gacctgttca aggccggcgt gatcgattcc ttcgggtaca
      781 tgcaattgat gcagtttctt gacagtgaat tcggtgtccg ctttgcggac gaagaattgc
      841 tgagcaacat cctggtcagc tacgatcatc tgatcgcatc ggtgcgtgcc agggtggcga
      901 cgcagggtgt ccgcgaaggc gcagcataag cgggatatcg cccaggaata tgggcatcgg
      961 ggagaactga tgtgtgggat ttccggattc tacggcgcgc ctccgaatgg tgtcgagccg
     1021 gccgaagcgg tcaagaagat gctggcgctg atccagcatc gagggcccga cgaggcgggt
     1081 tattacgtcg acgaccgcct ggcgatgggt accgtccgtc tcagcatcat cgatctcgcc
     1141 tccggtacgc aacccttgag cgatgccgac gagcgtcact ggatctgcta taacggcgag
     1201 ctgtacaact atcgggaatt gcgcagggaa ctggaaggtc acggctgccg gttccgcacc
     1261 caatcggaca ctgaagttgt gctgcaggcc tggctgttct ggggcgagca gtgcctgccg
     1321 cgtttgaacg gcgccttcgc ttttgccatt tacgacaatg ttgccggcag tctgctgctg
     1381 gcacgcgacc gttacggcaa gcgcccgctg ttccatgcac aacacgcggg agccttgatc
     1441 tttgcgtcgg agatgaaggc cttcctggcg ttcagggatt tccagttcgc agccgacccg
     1501 cagcaactgt cctcgatctt cgcgcagtgg acgccgctgc cggcgcagag cggcttcaag
     1561 gatatccacc agttgccgat ggcctgcttt ctgcgcgtcc aggagggtca gcgcagcgaa
     1621 ggttgctacg agcggctcga ctttgctcgc gggccggcgg tcgccagcga agcacaagcc
     1681 gtggagaggg tcaggcaggc gctgaccgac agcgtgcgct tgcgcttgcg cagcgatgtc
     1741 gaggtagggg tttacctgag cggcgggctg gactcgtcga tcattaccca actcgtcacg
     1801 cagatgtcgc cacatacgat acacaccttt tcggtggcct tcgaagacaa gtatttcgac
     1861 gagtcggagg accagagcga ggtcagtcgc tgtctcggta ccgagcacag tgc
//
LOCUS       DAOSIT010000482         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52037_length_1911_cov_9.739763, whole genome shotgun sequence.
ACCESSION   DAOSIT010000482 DAOSIT010000000
VERSION     DAOSIT010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52037_length_1911_cov_9.739763"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLL14_10210"
     CDS             complement(<1..830)
                     /locus_tag="PLL14_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG04344.1"
                     /translation="MSTATQQRISILVIEDDPGDLGLIQANVRLAGMRRLGDQDPVVW
                     APTLALGVVAAAARKPDVVLLDLSLPDSTGVATVEAMRVVVPDVPIIVLTGQDDHQLA
                     EAALKAGAQDYLVKGQFEHDALGRAIRHALVRQRLESRLRLFGAALNSVANGIVITDV
                     NATIEWANPAFTRMTGIPLAEALGRKPNELLNSGKQDAQFYQRMWETILAGRVWSGEL
                     INRRKDGTLYDELLTISPVTDVNGSIQHFVAVKQDISERKATEERVQHLAHHDQLTDL
                     P"
     gene            complement(866..1315)
                     /locus_tag="PLL14_10215"
     CDS             complement(866..1315)
                     /locus_tag="PLL14_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG04345.1"
                     /translation="MNQTPRALRILMIEDEAGDARLMQVAIRRNGFIAETHEAHDGHE
                     ALCFLRKQEPYRCGAVRPDLILLDLKMPGQGGLEFLATLKADTELRAIPVVVVTSSLL
                     DADVRAAYQLGAAGYLLKPADLREFIAKVRTLGEYWFNLVRLPHNAG"
     gene            complement(1341..>1911)
                     /locus_tag="PLL14_10220"
     CDS             complement(1341..>1911)
                     /locus_tag="PLL14_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04346.1"
                     /translation="AEQSEYFNFAIDGAKRLDRMLVALLEYSRVGRMGEPLTWIESRA
                     IVDEALLFLQPAIAEAEANISVCGKWPRVYVRPDGMLRVLQNLIGNATKFRVAGRAPQ
                     ITVASTVDGADWCLSVADNGIGIPAGQLSRLFQVFQRLQSRAAFEGTGVGLALCRKIV
                     EHHGGRIWAESAGEGQGSRFCLTLPLDGP"
BASE COUNT          347 a          632 c          568 g          364 t
ORIGIN      
        1 ggcaggtcgg tcaactggtc gtgatgggcc aggtgctgca cacgctcttc ggtggctttg
       61 cgttccgaaa tatcctgctt gacggcgacg aaatgctgaa tgctgccgtt gacgtcggtc
      121 accggcgaaa tggtcagcaa ctcgtcatag agcgtgccgt ccttgcgccg gttgatcagt
      181 tcaccgctcc acacacgccc ggcgagaatg gtttcccaca tccgctggta gaactgcgca
      241 tcctgcttcc cggaattgag caactcgttc ggcttgcggc ccagggcctc ggcgagcggg
      301 atgccggtca tcctggtgaa tgccgggttg gcccattcga tcgtcgcatt gacgtcagtg
      361 atgacgatgc cgttggccac cgaattcagc gcggccccaa acaggcgcag acgggattcg
      421 agtctttggc gcaccagcgc gtgccgtatc gcacgtccca gagcatcgtg ctcgaactgt
      481 cccttgacca ggtagtcctg cgcaccggcc ttcagcgccg cctcggcaag ctgatggtcg
      541 tcctggccgg taagcacgat gattggaacg tccggcacca cgacgcgcat cgcctcgacc
      601 gtcgccacgc ccgtcgaatc gggcagggac aggtcgagca acacgacatc cggcttcctg
      661 gcggcggcgg ccacaacgcc cagcgccagg gtcggcgccc agaccacggg atcctggtct
      721 ccaagccgcc gcattccggc caaccgtacg ttagcctgaa tcaatcccag atcgccggga
      781 tcgtcctcga tgaccaatat tgaaatgcgc tgctgggttg ccgtgctcat gcccattgct
      841 ccttagtgcc ccgtgcttcc agggttcaac ccgcattgtg cggcaagcgc accagattga
      901 accagtactc gcccaaagtg cgcaccttgg cgatgaattc gcgcaagtcc gccggtttca
      961 gcaagtaccc ggccgcacca agctgatagg cggcccgaac gtcggcgtcg agcagggaag
     1021 acgtcacgac gacaaccggt atggctcgca gttccgtatc tgccttgagg gtggccagga
     1081 actcaagtcc cccctgcccg ggcatcttga ggtccagcag gatcaggtcg gggcgcaccg
     1141 cgccacaccg gtacggttcc tgcttgcgca ggaagcatag cgcctcgtgc ccatcgtgtg
     1201 cctcatgcgt ctcggcgatg aagccattcc ggcgtattgc cacctgcatc aggcgggcat
     1261 cgccggcttc atcctcgatc atcaggatgc gcagggctcg tggcgtctgg ttcatgcgcg
     1321 tcgctccgtt tcactgggca tcatggaccg tccagcggca gcgtcagaca aaaacggctg
     1381 ccctgccctt cgccggcaga ctcggcccag atcctccccc catgatgctc gacaatcttg
     1441 cggcacaggg ccaatccgac tcccgtacct tcaaaagctg ctcgactttg caggcgttgg
     1501 aagacctgaa acaggcggct cagttgcccg gcagggatgc cgataccgtt atcggcaacc
     1561 gacagacacc agtcggcacc gtcgaccgtg ctcgcaaccg tgatttgcgg cgcgcgtccg
     1621 gcgacacgga acttggtggc gttgccgatc aggttctgca ggacgcgcag catcccgtcc
     1681 gggcggacgt aaacccgcgg ccacttgccg cagacgctga tgtttgcctc tgcctcggcg
     1741 atcgccggtt gcaggaagag taatgcttcg tccactatcg cgcggctttc aatccatgtc
     1801 agcggttcgc ccatgcgtcc gacgcgcgag tattcgagga gcgccaccaa catccggtcc
     1861 agacgcttgg cgccatcgat ggcaaagttg aaatactcgc tttgctcggc a
//
LOCUS       DAOSIT010000483         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52071_length_1910_cov_12.554717, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000483 DAOSIT010000000
VERSION     DAOSIT010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52071_length_1910_cov_12.554717"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1910
                     /locus_tag="PLL14_10225"
     CDS             <1..>1910
                     /locus_tag="PLL14_10225"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOG04347.1"
                     /translation="ELEILSSRMVLGRVVNKLKLDLVAVPRTLPLIGGAIARRYQGDE
                     PNSPWLGLTSFAWGGERIQVDSFDVPADALDDRHTLIATAGGGFELFDEDEQLVLKGT
                     VGKRAGDRGYSIFVADLQARPGTQFRLIKRSAEATIDDLRRNYSVKERGKKSGVLELS
                     LLGQDPQQIGVILDDILNTYVRQNVERRSAEAEKTLKFLETQLPALKTQVDSAEAAYN
                     NYRQSRGSLDLSLETQGILKSLVEVENAAVLLKQERDELRQNFTPEHPRIQAMDNKLQ
                     RLNERRRQFESEVSRLPDTQQTVLRLARDVEVSTKLYTELLNTAQQLRVSKAGTVGDV
                     RVIDMAAVARKPVGAGAVAILAIALLLGLLFSLVVIWLLRSLRVVVEDPEIIEAQLGL
                     PVYATVPHSKAELELHRKAQSGVGELLAVVHPQEDAVESLRGLRTTLHFALLDAQHNS
                     LLITGPSPGLGKSFISKNLGVVLAQVDKSVVIVDGDLRRGHLHKEFAIERAPGVSEYV
                     AGQASLEDILKPTSVPKLWVVTTGQIPPNPSELLMHLRFEALLVELGARFDTVIVDAP
                     PVLAVSDAAIIGRHVGATLMVARAGRHPIRELEQAVKRLNQAGVQVKGFVFNDLNVSR
                     QSYRYGYKGYVYR"
BASE COUNT          346 a          621 c          619 g          324 t
ORIGIN      
        1 gaactcgaaa tcctcagttc gcgaatggtt ctcggacgcg tggtcaacaa gctcaagctc
       61 gacctcgtcg ccgtgccgcg gacgctgccg ctgatcggcg gtgcgattgc ccggcgctac
      121 cagggcgacg agccgaattc accgtggctg ggactcacca gtttcgcctg gggcggcgaa
      181 cggattcagg tcgactcgtt cgacgttccc gccgatgcgc tcgacgaccg acataccctg
      241 atcgccacgg cgggcggggg cttcgagctc ttcgacgagg acgagcaact ggttctgaag
      301 ggtacggtcg ggaagcgcgc cggcgaccgc ggctactcga tcttcgtggc cgacctgcag
      361 gcgcgtccgg gtacgcagtt ccgcctgatc aagcgctccg ccgaagcgac gatcgacgat
      421 ctgcgtcgca actattcggt gaaggaacgc ggcaagaagt ccggcgtcct cgaactgagc
      481 ctgctcggac aggatcccca acagatcggc gtgatcctcg acgacatcct caacacctac
      541 gttcgccaga acgtcgagcg gcgctcggcc gaagcggaaa agaccctcaa gttcctggaa
      601 acgcagctac cggcgctcaa gacgcaggtg gacagcgccg aggcggccta caacaactat
      661 cgccagagcc gcggctcgct cgatctcagc ctggagacgc agggcatcct gaagtcgctg
      721 gtcgaggtcg aaaacgccgc cgtgctgctc aagcaggaac gtgacgaact gcggcagaac
      781 ttcacgccgg agcacccgcg gatccaggcg atggataaca agctgcagcg tctcaacgag
      841 cgccgccggc agtttgaatc agaggtgtcg cgtctgcccg atacgcagca gaccgtcctg
      901 cgtctcgccc gcgacgtgga agtgtcgacc aagctgtata ccgagctgct caacacggcg
      961 cagcagttgc gcgtctcgaa agccggtacg gtcggcgacg tgcgcgtcat cgacatggct
     1021 gccgtcgcgc gcaagccggt cggcgccggc gcggttgcca tcctggcgat cgccctgctg
     1081 ctcggcttgt tgttcagcct ggtggtgatc tggttgctgc gctcgctgcg cgtggtcgtc
     1141 gaggacccgg agatcatcga ggcgcagctc ggtctgccgg tctatgccac ggtgccgcac
     1201 agcaaggccg aactggaatt gcatcgcaag gcacaaagcg gtgtcggcga gctgttggcg
     1261 gtcgtccatc cgcaggaaga cgcggtcgag agcctgcgcg gactgcgcac gacgctgcac
     1321 ttcgcgctcc tcgacgcgca gcacaactca ctgctgatca ccggcccgag cccgggtctg
     1381 ggcaagagct tcatctccaa gaaccttggc gtggtcctgg cgcaggtcga caagagtgtc
     1441 gtgatcgtcg atggtgacct gcgccgcggg cacctgcaca aggaattcgc gatcgagcgc
     1501 gcgccgggcg tttccgagta cgttgccggc caggcttcgc tcgaagacat cctgaagccc
     1561 acctcggtgc ccaagctgtg ggtggtcacc accggccaga ttccgccgaa tccttccgaa
     1621 ctgctgatgc acctgcgttt cgaagcactg ctggtcgaac tcggggcgcg tttcgacacc
     1681 gtgatcgtcg atgcgccgcc ggtgctcgcg gtctccgacg cggcgatcat cgggcggcat
     1741 gtgggagcca ccctgatggt cgcccgtgcc ggcaggcacc cgattcgcga gcttgagcag
     1801 gccgtcaagc ggctcaatca ggcgggcgtg caggtcaagg gctttgtctt caacgacctc
     1861 aacgtcagcc gccagagcta ccgctacggc tacaagggct acgtttaccg
//
LOCUS       DAOSIT010000484         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52074_length_1910_cov_8.547709, whole genome shotgun sequence.
ACCESSION   DAOSIT010000484 DAOSIT010000000
VERSION     DAOSIT010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52074_length_1910_cov_8.547709"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLL14_10230"
     CDS             complement(<1..430)
                     /locus_tag="PLL14_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04348.1"
                     /translation="MSEPQSLGLDFMADDTLSGFRLQRLEVFNWGTFDGRVWTLSPEG
                     RNALLTGDIGSGKSTLVDAVTTLLVPAQRIAYNKAAGADSRERTLRSYVLGHYKSERN
                     ELSGTARPVALRDHNNYSVILGVFHNAGYDQTVTLAQVFWI"
     gene            complement(423..1067)
                     /locus_tag="PLL14_10235"
     CDS             complement(423..1067)
                     /locus_tag="PLL14_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOG04349.1"
                     /translation="MMQEDLEASAVTVAASPDLSALVIPLLKGVIYQDADAGLWNALL
                     TLQARVRDYVAVLNLELVFDEAEGYAFLRSRPEPEDGLAPRLPRLVARRPLSFPVSLL
                     LALLRKKLAEFDASGGDTRLVLARDEVVELMRVFLPEGSNEARLIDQIDAHLGKVIEL
                     GFLRRLKVGTGQPAMVEVRRILKAFVDAQWLAEFDQRLAGYRALLAGQPGVADE"
     gene            complement(1064..>1910)
                     /locus_tag="PLL14_10240"
     CDS             complement(1064..>1910)
                     /locus_tag="PLL14_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOG04350.1"
                     /translation="GAVYHVTSRGDRREDIFLGDDDRRDWLEVLGLACARFNWVVHAY
                     CQMTNHYHLLVETVDGNLSSGMRQLNGLYTQRFNRRHGLVGHLFQGRYKAILVQKEAH
                     LLELTRYVVLNPLRAKLVESLEDWRWSSYPCTAGQEAPPPWLDTDGLLGQFGPNRGEA
                     LRSYGQFVMAGAGLPSPMLATRHQLLLGDKAFVERHSQTRNAEDLREVSRAHRRSIAL
                     SLDEYGARHADRDQAMAEAYLSGAYTMAEIGRHFAVHYMTVSRAVRKFEQDRKNMIFK
                     QAPAE"
BASE COUNT          376 a          646 c          553 g          335 t
ORIGIN      
        1 tgatccagaa cacctgcgcc agcgtcaccg tctggtcgta accggcgttg tgaaaaacgc
       61 cgaggatcac cgagtagttg ttgtggtcgc gcagcgccac cgggcgggcc gtaccgctga
      121 gttcgttgcg ctcggatttg tagtggccga gaacgtacga gcgcaacgtc cgttcgcggc
      181 tgtcggcacc ggcggccttg ttgtaggcga tacgctgcgc cggcaccagc agcgtcgtca
      241 ccgcatcgac cagcgtcgac ttgcccgaac cgatgtcgcc ggtcagcagg gcgttcctgc
      301 cctccggact gagcgtccac acacggccgt cgaaagtgcc ccagttgaac acttcgagac
      361 gctgcaggcg gaaacccgaa agcgtgtcat cggccatgaa atccaggccg aggctttgcg
      421 gctcactcat cggcaacgcc cggctgcccg gcgagcagcg cgcgatagcc ggccagccgc
      481 tgatcgaact cggccagcca ttgcgcatcg acgaatgcct tgagaatccg ccgcacctcg
      541 accatcgccg gctgtccagt gccgaccttc aggcgacgca ggaacccgag ttcgatgacc
      601 ttgccgagat gggcatcgat ctggtcgatc agccgcgcct cgttgctgcc ttcgggcagg
      661 aaaacccgca tcagctcgac gacctcgtcg cgcgccagta ccaggcgcgt atcgccgccg
      721 ctggcgtcga actcggccag tttcttgcgc agcagcgcga gcaacagact gaccggaaac
      781 gacaacgggc gccgcgccac cagccgcggc aggcgcggtg ccaaaccgtc ctcgggttcc
      841 ggacgcgagc gcaggaaagc gtagccctcc gcttcgtcga acaccagttc gaggttgagc
      901 accgccacgt agtcgcgcac ccgcgcctgc agtgtcagca gcgcattcca cagaccggcg
      961 tcggcgtcct ggtagatcac gcccttgagc aggggaatca ccagtgccga cagatccggg
     1021 ctggcagcca ccgtcacggc agaggcttcg agatcttcct gcatcattcc gccggcgcct
     1081 gtttgaagat catgttcttt cggtcttgtt caaacttccg caccgcgcga cttacggtca
     1141 tgtagtggac cgcgaagtgg cgcccgatct ctgccatcgt ataagcaccg gataggtaag
     1201 cctctgccat cgcctgatct ctatcggcgt ggcgcgcccc gtattcatcc agcgacaagg
     1261 cgatcgatcg tcggtgcgcc ctggaaactt cgcgcagatc ctctgcgttc ctggtctgcg
     1321 aatggcgctc cacgaacgcc ttgtcaccca gcaacaattg atggcgagtg gccaacatcg
     1381 gactaggcaa gcccgcgccc gccatcacga actgaccata gctcctgagc gcttcgccgc
     1441 ggtttggacc aaactgaccc agcaacccat ccgtatccag ccacggcggc ggtgcctcct
     1501 ggccggcagt gcaagggtaa ctgctccagc gccagtcctc aagcgattcc accagcttgg
     1561 cgcgcagtgg gttcagcacg acgtagcgag tcaactccag caagtgggct tccttttgta
     1621 cgaggatcgc cttgtagcgc ccctgaaaga gatggccaac caggccatgg cggcggttga
     1681 aacgctgcgt ataaaggccg ttcaattgcc gcatcccact cgatagatta ccgtccacag
     1741 tctccaccag caggtgatag tgattggtca tctggcaata ggcatgcacc acccagttga
     1801 accgcgcgca agcaaggccc agcacctcca gccaatccct gcgatcatcg tcgcccagaa
     1861 aaatatcttc ccgacgatca ccgcgcgagg tgacgtgata tacagcaccc
//
LOCUS       DAOSIT010000485         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52377_length_1902_cov_6.920953, whole genome shotgun sequence.
ACCESSION   DAOSIT010000485 DAOSIT010000000
VERSION     DAOSIT010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52377_length_1902_cov_6.920953"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLL14_10245"
     CDS             <1..512
                     /locus_tag="PLL14_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG04351.1"
                     /translation="TLLAAHRRLRATGRWIDAALRSMLQSNRLAASCLNTYAASACTR
                     VSALGLHGQLLAMTRRAGVAVELDLNALPVLDGAAETAALGFPSWRPAAGSCTSRAPS
                     ASDPACRHPKHALLFDPETSGGLLASVPPETAEACLQALRALGYPRATSVGRVLPAGQ
                     DPQPILLKL"
     gene            703..>1902
                     /locus_tag="PLL14_10250"
     CDS             703..>1902
                     /locus_tag="PLL14_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOG04352.1"
                     /translation="MFDNLRKYAAAYAVAIGGVLLTCLSSWNVRQELQTSHFKEFQWA
                     AGDRIQSVRAVVDQGLDALLEIRGLFYAAQGVDEREFLVFTDSILKRRPYIDSLFWAP
                     LLTSPRQALPGSAPGSMARAAPVAGQAEAAGTLRVPVLLSASRSGTGLARGVDLNSMR
                     ELAVLFQRARASGNVAVSGRMEMARPGRESAFVIYAALPVYAPGATGTAGTTKYREPL
                     GFVIGVYDIEELVHVAISLLEPRGVEVLVRDDSAAGDAQFLHFYASRLEPRAAATASG
                     LMPEGDEDQPRMVVRIPVGDRSWTVTCVATHTFRSAEVFTKAHWSVLLGGILFTALLT
                     FYLVRSRRELENRIQLTQTIYEREELFRQLAETVDVVFWATNAAATRLEYIGPAFRQI
                     AGREARLD"
BASE COUNT          302 a          588 c          638 g          374 t
ORIGIN      
        1 cgaccctgct cgctgcgcat cgccgtctgc gggctacggg ccgctggatc gatgcggcgc
       61 tgcgatcgat gctccagtcc aaccggctgg cagccagctg tctgaacacc tacgccgcca
      121 gtgcctgcac cagggtcagc gctcttggtc tgcatggcca gttgctcgcg atgacccgcc
      181 gtgcgggcgt ggctgtcgaa cttgatctga acgccttgcc ggttctcgat ggcgctgcag
      241 agactgctgc tctgggtttc ccgagctggc ggccagcggc cggttcgtgc acgagccgcg
      301 ctccaagcgc gtctgatcct gcttgccgtc acccgaagca tgcgttgttg ttcgatccgg
      361 aaacgtcagg agggctgctg gccagcgtac ctccagagac tgcagaggcg tgtctgcagg
      421 cgctgcgcgc tctcggttac cctcgggcca ccagcgtggg gcgggtgttg ccggccggcc
      481 aggatcctca gccgatcctg ctcaagctgt aaacattcgg tggcgagaag cgccatccgg
      541 acccggcggg tacggcacga tctcgtgccc tgaggaatgc gacgacgttt ccccgctgtt
      601 cttgccggac acgctttctg ctaagcttcc ggggaacagg tcgtggcgat tcgggagttg
      661 cgcccgcgca gccctgtacc gcaaccctct gatggccgcc ccatgttcga caacttgcga
      721 aagtatgccg ccgcctacgc cgtggcgatc ggtggagttc tcctgacctg cctgtcgtca
      781 tggaacgtgc ggcaggagct gcagaccagc cacttcaagg aattccagtg ggcagccggt
      841 gaccgcatcc agtccgtgcg agcggtggtc gaccagggcc tggacgcgct gctggaaatt
      901 cggggcctgt tctacgcggc gcagggagtc gacgaaaggg agttcctggt ttttaccgat
      961 tcgattctca aacgccgccc gtatatcgac agtcttttct gggcgccgct gctgacctcg
     1021 ccgcggcaag ccctgcctgg atcggcaccc ggcagcatgg cgcgggcggc gccggttgca
     1081 ggccaggcgg aggctgccgg cacgttgcgg gtgccagtac tgctttccgc atcgcgttcg
     1141 ggaaccggtc ttgcgcgggg tgtcgatctg aactcgatgc gtgaactggc ggtgctattc
     1201 cagagggcaa gagcgagcgg caacgtcgcg gtcagcggac gcatggaaat ggcacgaccg
     1261 ggaagggaat cggcgtttgt catctatgcg gcactgcccg tctatgcgcc gggcgccacg
     1321 ggcactgccg gaacaacgaa ataccgggaa ccgctgggat tcgtgattgg tgtgtacgat
     1381 atcgaagaac tggtgcatgt ggccatcagc ctgcttgagc cgcgcggcgt cgaagtactg
     1441 gtgcgtgacg attcggcggc cggtgatgcg cagttcctcc acttctacgc cagccgcctg
     1501 gagccgcgtg ctgcggccac ggctagcggt ctgatgccgg agggcgacga ggatcagccg
     1561 cggatggtgg tcaggattcc cgtcggcgat cgtagctgga cggtgacctg cgtggcaacc
     1621 catacctttc gcagtgccga agtctttacc aaggcccact ggtcggtgct tctgggaggt
     1681 atcctgttca cggcgctgct cactttctac cttgtgcgca gtcgtcgcga actggaaaat
     1741 cggattcagt tgacgcagac catctacgag cgcgaagagc tgtttcgtca gttggccgaa
     1801 acggtcgacg tcgttttctg ggcgaccaac gctgctgcga cgcggctcga gtacatcggc
     1861 ccggcgtttc gccagatcgc cgggcgcgag gccaggctcg at
//
LOCUS       DAOSIT010000486         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52378_length_1902_cov_6.744450, whole genome shotgun sequence.
ACCESSION   DAOSIT010000486 DAOSIT010000000
VERSION     DAOSIT010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52378_length_1902_cov_6.744450"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1303
                     /gene="purD"
                     /locus_tag="PLL14_10255"
     CDS             23..1303
                     /gene="purD"
                     /locus_tag="PLL14_10255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG04353.1"
                     /translation="MKLLVIGSGGREHALAWRLAKTPGLQKVFVAPGNAGTARERELE
                     NVNLSDPEALADFAARQRVHLTVVGPEAPLAAGIVNVFRARGLKIFGPTREAAQLESS
                     KDFAKRFMARHNIPTARYRTFTDRVAAQAYIDQQGTPIVIKADGLAAGKGVVVAMNLA
                     EAHAAIARMLPDNPSADPADGSAARVVIEEFLEGEEASFIVMVDGKNALPLASTQDHK
                     RIGDGDTGPNTGGMGAYSPAPVVTPEVHARAMREIILPTVRGMAADGIPYTGFLYAGL
                     MIGKDGSVKTVEFNCRLGDPETQPIMMRLKSDLLNLLEHAVAGRLNLVEASWDRRIAL
                     GVVLAAAHYPDTPRTGDLISGLPPASDDSHVFHAGTAEKDGQIVTAGGRVLCVTTLSD
                     NLKQAQKRAYDLIVGIHFDGMQYRRDIGHRALSR"
     gene            1347..>1902
                     /locus_tag="PLL14_10260"
     CDS             1347..>1902
                     /locus_tag="PLL14_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOG04354.1"
                     /translation="MIDAARLQAYFTGLQARLMATLEAVEGEAGARFRVDPWVKEAGA
                     GLAGNGCTCIIEGGRVFERGGVAFSQVSGASLPASATAKRPELAGRAFAAMGVSLVLH
                     PENPYCPTAHMNVRALITTRPDAEPAWWFGGGMDLTPYYPFADDIRHFHSTCKQALLP
                     FGEGTHARYKKWCDEYFFLKHRNEP"
BASE COUNT          370 a          635 c          576 g          321 t
ORIGIN      
        1 ctctggcggt gaaaggatcc ttatgaagtt actggtcatc ggttccggcg gtcgcgaaca
       61 cgcactcgcc tggcgtctgg cgaaaacccc tggactacag aaagtcttcg tcgcccccgg
      121 caatgccggg actgcccgcg agcgggaact cgaaaacgtc aacctcagcg atccggaagc
      181 actcgccgat ttcgctgcca gacagcgcgt ccaccttacg gtagtgggtc cggaagcgcc
      241 gctcgccgcc ggtatcgtca atgtcttccg cgcacgcgga ctcaagatct ttggtccgac
      301 cagggaagcg gcgcaactcg agagttcgaa ggatttcgcc aaacgcttca tggccaggca
      361 caatatcccg accgcgcgct acaggacctt caccgaccga gttgcggcac aagcctatat
      421 cgaccagcag ggcacgccga tcgtcatcaa ggccgacgga ctggccgcag gcaagggcgt
      481 agtggtggcc atgaacctgg ccgaggcgca tgccgccatc gcacggatgc tcccggacaa
      541 cccatcggcc gacccggcgg acggttccgc ggcacgcgtc gtcatcgaag agttcctgga
      601 aggcgaagag gcgagcttca tcgtcatggt cgatggcaag aacgcccttc ccttggcatc
      661 gacccaggat cacaagcgaa tcggcgacgg cgataccggc cccaacaccg gcggcatggg
      721 cgcctactca ccggcaccgg tggtcactcc ggaagtgcac gcccgcgcca tgcgcgagat
      781 catcctgccc acggtgcggg gcatggctgc cgacggcatc ccctataccg gcttcctcta
      841 cgccggcctg atgatcggca aggacggatc ggtgaagacg gtagagttca attgccgcct
      901 gggagacccc gaaacccagc cgatcatgat gcgcctgaag tccgacctgc tcaacctgct
      961 cgaacatgcc gtcgccggcc gactcaatct ggttgaagca agctgggatc gccgcatcgc
     1021 gctcggcgtc gtgctcgccg cagcccacta cccagacacg ccacgcaccg gcgatctgat
     1081 cagcggcttg cctccggcca gcgacgacag ccatgtcttc catgccggaa cggccgaaaa
     1141 agatggccag atcgtcaccg ccggcggccg tgtgctgtgt gtcacgaccc tgtccgacaa
     1201 tctcaagcag gcacagaagc gtgcctacga tctgattgtc ggcattcact tcgacggcat
     1261 gcagtatcgc cgcgacatcg gccaccgcgc cctcagccga tgaacctgtg gcaagcacag
     1321 cgggcccgtt gcgtggcgcg taaaggatga tcgatgcggc ccggctacag gcctacttca
     1381 cgggtttgca ggcgcgcctc atggcgacac tcgaagcggt cgagggtgaa gctggggccc
     1441 gcttccgggt tgacccctgg gtaaaggaag ccggcgctgg cctcgcgggc aacggttgca
     1501 cctgcatcat cgagggtggt cgcgtattcg agcggggcgg ggtcgccttc tcgcaggtca
     1561 gtggcgcctc cctgccggcc tcggcaaccg ccaagcgccc cgaactcgcc ggccgtgcct
     1621 tcgccgccat gggcgtttca ctggtgttgc atccagaaaa cccttactgt ccgacggcac
     1681 acatgaacgt ccgggcgttg atcactacca ggccggatgc agagcctgcg tggtggtttg
     1741 gcggcggcat ggatctgacg ccatattatc ctttcgctga cgacatccgg cattttcaca
     1801 gcacctgcaa gcaagccctg ctgcctttcg gagaaggaac gcatgcccgc tacaagaaat
     1861 ggtgcgacga gtatttcttc ctcaagcacc gcaacgaacc gc
//
LOCUS       DAOSIT010000487         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52616_length_1896_cov_8.907659, whole genome shotgun sequence.
ACCESSION   DAOSIT010000487 DAOSIT010000000
VERSION     DAOSIT010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52616_length_1896_cov_8.907659"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..358)
                     /locus_tag="PLL14_10265"
     CDS             complement(59..358)
                     /locus_tag="PLL14_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOG04355.1"
                     /translation="MPETPKTLVTRSGRVLDLPAPEEDARIAAGIAADSDTYELGAAE
                     FERLRRPGRPRAAVKRPMLSMRADPDVLAHLRASGKGWQTRVNALLREAVEQGRL"
     gene            complement(342..614)
                     /locus_tag="PLL14_10270"
     CDS             complement(342..614)
                     /locus_tag="PLL14_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOG04356.1"
                     /translation="MRIEFDLAKDVSNERKHGVGLALAADLEWDTLLSRPDTRREYGE
                     SRQIGYAIAGRRLYCVVFVDRAGDVRRIISLRKANSREVASYARNA"
     gene            complement(753..>1896)
                     /locus_tag="PLL14_10275"
     CDS             complement(753..>1896)
                     /locus_tag="PLL14_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG04357.1"
                     /translation="NDRHLPDKAIDVIDEAGAAQRILVASKRKKTIGKAEIEEIVAKI
                     ARIPPANVSNDDRGKLQTLERDLKSVVFGQDKALEVLASAVKMARSGLGRADKPIGSF
                     LFSGPTGVGKTEAAKQLAYIMGIELMRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLL
                     TEAVTKKPHAVLLLDEIEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNIIIIMTTNA
                     GAETMNKATIGFTNPRVAGDEMGDIKRLFTPEFRNRLDAMVSFKALDEVVIMRVVDKF
                     LLQLETQLAEKKVDVTFTDKLRKHLAKKGFDPLMGARPMQRLIQDTIRRALADELLFG
                     RLTDGGRLTVDIDDKDEVQLDIHPLPKKEGKGTRQEPAEPEQATAG"
BASE COUNT          300 a          607 c          611 g          378 t
ORIGIN      
        1 gcgtggaatg gccataaaca gctatcgttt caggagtagt ctggcgccgc tgcgcctgtt
       61 acagccggcc ctgctccacc gcctcgcgca gcagcgcatt gacgcgcgtc tgccagcctt
      121 tgccgctggc gcgcagatgg gccagcacat cggggtcggc acgcatgctc agcatcgggc
      181 gcttgacggc cgcgcgtggg cgccccggac ggcgcagccg ctcaaactcg gcagcgccca
      241 gttcataggt gtcggaatcc gctgcgatgc cagcagcgat gcgagcatcc tcttcgggcg
      301 cgggcaaatc cagcacccgg ccggagcggg tgacaagggt cttaggcgtt tcgggcataa
      361 ctggcaacct ctcgtgaatt ggcctttcgc aggctgataa tccggcgcac gtcgccggcc
      421 cggtccacaa acaccacgca gtacaagcgc cgcccggcaa tggcgtatcc aatctggcgc
      481 gactcgccat actcgcggcg ggtgtcaggg cggctcagca gcgtgtccca ctccaggtcg
      541 gccgccaagg ccagcccaac gccatgtttg cgctcgttgg acacatcctt ggctaggtcg
      601 aactcgatcc gcattgactt tattgtattg acatcaatat atttgtcaat acattaattc
      661 aagtggcgat gagctgcaaa gacagcacct gaccgaacaa aaaaaagccg gcactaggcc
      721 ggcgtctttt gcgcggagga tgtgcggttg cctcagcccg ccgtggcctg ctcgggctcg
      781 gcaggctcct ggcgggtgcc cttgccttct ttcttgggca gcggatgaat gtcgagctgc
      841 acctcgtcct tgtcgtcgat gtccaccgtc aggcgtccgc catcggtgag gcggccgaac
      901 agcagctcgt cggccagggc gcggcggatg gtgtcctgga tcaggcgctg catcgggcgc
      961 gcgcccatca gcggatcgaa gcccttcttg gccaggtgct tgcgcagctt gtcggtgaag
     1021 gtgacgtcga ccttcttctc ggcgagctgc gtctccagct gcagcaggaa cttgtccacc
     1081 acgcgcatga tgaccacttc gtcaagcgcc ttgaagctca ccatggcatc cagacggttg
     1141 cggaactcgg gcgtgaacag gcgcttgatg tcgcccatct cgtcgcccgc cacgcgcgga
     1201 ttggtgaagc cgatggtggc cttgttcatg gtctcggcgc ccgcgttcgt cgtcatgatg
     1261 atgatgatgt tgcggaagtc ggccttgcgc ccgttgttgt cggtcagcgt gccgtggtcc
     1321 atcacctgca gcagcacgtt gaagatgtcc ggatgcgcct tctcgatttc gtcgagcagc
     1381 agcacggcgt gcggcttctt cgtcaccgcc tcggtcagca ggccgccctg gtcaaagccc
     1441 acgtagccgg gcggcgcgcc aatcaggcgg ctgacggcgt ggcgctccat gtattccgac
     1501 atgtcgaagc gcatcagctc gatgcccatg atgtaggcga gctgcttggc ggcttcggtc
     1561 ttgcccacgc cggtggggcc ggagaacagg aaggagccga tcggcttgtc cgcgcggccc
     1621 aggcccgagc gcgccatctt gacggcgctg gccagcactt ccagggcctt gtcctgccca
     1681 aagacgacgc tcttgaggtc gcgttccagc gtctgcagct tgccccggtc gtcgttggag
     1741 acgttggccg gcgggatgcg ggcgatcttg gccacgatct cttcgatctc ggccttgccg
     1801 atggtcttct tgcgcttgga cgcgaccagg atgcgctggg ccgcaccggc ctcgtcgatc
     1861 acgtcgatgg ccttgtcggg caggtgccgg tcgttg
//
LOCUS       DAOSIT010000488         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52865_length_1889_cov_9.050164, whole genome shotgun sequence.
ACCESSION   DAOSIT010000488 DAOSIT010000000
VERSION     DAOSIT010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52865_length_1889_cov_9.050164"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLL14_10280"
     CDS             <1..666
                     /locus_tag="PLL14_10280"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HOG04358.1"
                     /translation="FRIATAARVTNLDSQTLNALSPLPAVPFGNFLGSSNTVPLIGDL
                     GETRAHDTRSLLRFELGALALTADRVRNAKFNLFALPGLPPFADPTAAKPVTTELHRV
                     TEAWSETAVTWETQPAVAGTFTSTVQSGVNQWVSFDVTALVQDWLTDPSSNFGFELLQ
                     PDIVIADSGKPIASLYASSASANAALRPFLEITAIPEPPTPTLLLGALGLVGAISRWR
                     KAV"
     gene            complement(780..>1889)
                     /locus_tag="PLL14_10285"
     CDS             complement(780..>1889)
                     /locus_tag="PLL14_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG04359.1"
                     /translation="SEEMAALALAYVARTRRQSDQLASVYFADTELAYRDDNRHLWRF
                     HELSDDEEMFDQVRPASAGTELDRLPPRHYPEWDYQTQSYRPDWVSLYESLHPSGDAG
                     HIDRLLEKHAPLARRLKRLLDLLRPQERTRIRYQEDGSELDLDIAIRSLIDFKSGANP
                     EPRINMSHRTNGRNLAVLLLLDLSKSLAERVEVGNGVPGQSILELSQEAVSLLAWSIE
                     QLGDPFAIAGFHSDTRHDVRYLHLKGYSESWGDLVKARLAAMQAGYSTRMGAAMRHAA
                     HYLAARKAEKRLLLVLTDGQPADVDVPDERLLIEDARQAVGELDQKGIFTYCISLDRK
                     ADVYVGDIFGRRYAVIDNVERLPERLPELFLALTR"
BASE COUNT          339 a          605 c          577 g          368 t
ORIGIN      
        1 ttccgtatcg ccaccgccgc gcgcgtgacc aacctcgaca gccagacgct gaacgcgctg
       61 agccccctac ccgccgtacc cttcgggaat tttctgggta gcagcaacac cgtcccgctg
      121 atcggcgatc tgggcgagac gcgcgcgcat gacaccagat cgctgttgcg tttcgaactc
      181 ggagcgctgg cgctcactgc cgaccgggtc aggaacgcga agttcaacct ctttgccctc
      241 cctggtctgc caccgttcgc cgacccgaca gcagccaaac cagtcaccac cgagttgcat
      301 cgcgtaaccg aagcctggag cgagactgcc gttacctggg agacgcagcc tgccgtcgcc
      361 gggacgttca cctcgaccgt gcaaagcggc gtgaatcaat gggtgtcgtt cgatgttacg
      421 gcgctggttc aggactggct tacggacccg tcttcaaact tcggtttcga actgttgcag
      481 ccggacatcg tcattgccga ctccggcaaa ccgatcgctt cgctgtacgc gtcttccgcc
      541 tcggccaatg cggcgctgcg tcccttcctg gaaatcacgg caatccccga gccacccaca
      601 ccaacgttgc tgctcggcgc actcggcctc gtcggcgcca tttcgcggtg gcgcaaggca
      661 gtctgagtca gagtggtgtg gcccggggac tccgtcggca aaataccccg atcgcgcaaa
      721 agtagacgac aggaggttgt gccaagcctg tagcgtgggg tctcttgcaa gactccgcgc
      781 tacctggtca gggccaggaa taactccggc aatctctccg gcaaccgttc gacattgtcg
      841 atcaccgcgt accgccgccc gaagatatcg ccgacataca catcggcttt gcggtcgagg
      901 ctgatgcagt aggtgaaaat acccttctgg tcgagctctc cgaccgcttg acgagcgtct
      961 tcgatgagca ggcgctcgtc gggcacatcc acatctgccg gttggccgtc ggtgaggacc
     1021 agcagcagtc tcttctcggc cttgcgggca gcgaggtagt gtgcggcgtg acgcatggct
     1081 gcgcccatgc gtgtcgaata gccggcctgc atcgctgcga ggcgagcctt gaccaggtcg
     1141 ccccagcttt cgctgtagcc cttgaggtgc aggtaacgaa catcgtggcg ggtgtcggaa
     1201 tgaaaaccgg cgatggcgaa ggggtcgccg agttgctcga tcgaccaggc gagcaacgac
     1261 accgcttcct gactcagttc gagaatgctc tgcccaggga caccgtttcc gacctcgacc
     1321 cgctcggcaa gcgacttcga cagatcgagc agcaacagca cggcgagatt gcgaccgttg
     1381 gtgcgatggc tcatgttgat tcgcggttca gggttggcac cgctcttgaa atcgatcagc
     1441 gaacgaatgg cgatgtcgag atcgagttcg ctgccatcct cctggtagcg tatgcgcgtt
     1501 ctctcctgcg gcctgaggag gtcgagcagg cgcttcagac gcctcgccaa cggcgcgtgt
     1561 ttttccagca ggcggtcgat gtgaccggcg tcgccactcg gatgcagcga ttcgtaaagg
     1621 ctcacccaat cgggccggta gctctgggtc tggtaatccc actccggata gtggcgtggc
     1681 ggcaggcggt cgagttcggt ccccgcggac gccgggcgca cctgatcgaa catctcctcg
     1741 tcgtcgctca actcgtggaa tcgccacagg tggcgattgt cgtctcggta ggccagctcg
     1801 gtatccgcga aatacacgct cgccagttga tcgctctgtc gacgggtgcg cgcgacgtag
     1861 gccagcgcga gagcagccat ttcttcgct
//
LOCUS       DAOSIT010000489         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52866_length_1889_cov_8.998909, whole genome shotgun sequence.
ACCESSION   DAOSIT010000489 DAOSIT010000000
VERSION     DAOSIT010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52866_length_1889_cov_8.998909"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLL14_10290"
     CDS             complement(<1..269)
                     /locus_tag="PLL14_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04360.1"
                     /translation="MQSYDATLAEGARVLVDSLAGDPPLHRSATTDGEARLSTGRLGE
                     NRILELAAPDVRILDVDFNADRRQDLVVIGERPDGNAFARLWQATG"
     gene            760..1254
                     /locus_tag="PLL14_10295"
     CDS             760..1254
                     /locus_tag="PLL14_10295"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG04361.1"
                     /translation="MSAFPVNDGTPQYGTASGVGSEQRPLPKVESSALAARLQAIIGK
                     ESVSSFARRCGLAESVLRTYLRDGRMPPLDKACAMAAAAGVSVDWLATGNGPGRAAEV
                     RASYHSGAPGVVASDTRPVDTGVLEGILKAVLEAQDTHAPPAYLAALIADLYRRAMDR
                     HLPA"
     gene            1444..>1889
                     /locus_tag="PLL14_10300"
     CDS             1444..>1889
                     /locus_tag="PLL14_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG04362.1"
                     /translation="MNNTSSFSRDSRAAWLLAGVELFFSLSWVVYVIFLPELLLRGGV
                     DKHYLPWLLAVDQLLFALADWSMGVAVDRARAALRRIGPMLVLLSAVSALAMLLLPWL
                     SGMPMLFLLMTGLWVATSSALRAPPYVLLSRYAGRSSVAGLAGIQLL"
BASE COUNT          307 a          556 c          616 g          410 t
ORIGIN      
        1 ccagtggctt gccacagcct ggcaaaggcg ttgccgtcgg gccgttcgcc gatgacgacg
       61 agatcctggc gtcggtcggc gttgaagtcg acatccagga tgcggacatc cggcgcagca
      121 agttccagga tccggttctc gccaaggcgg ccagtggaga gccgtgcttc gccatcggta
      181 gtggcggacc tgtggagcgg tgggtcacca gccagactat cgacaagcac ccgcgcgccc
      241 tcggcaaggg tcgcgtcgta gctttgcacc tccagatgtg tcccagccgt cccgatggtt
      301 gccatcggaa gcgaaaccgg atcgcccgat aacagcagtc gcggctcgat ggcttcgaaa
      361 tgaatgcgcg gactggtttc gcaggctctg gcggaggagg gggggttgcc gcaagcctgc
      421 cctggcgatg attgttttcc ctgcaaggca gggaaaactc gacgcagcgc agacagtagc
      481 ggtgcgagtg aaaatccggg tgggctatcg ggacggtcgt ttgcttctac agactggcgc
      541 atggctattc cccctcgcat cgcgatgcaa tgccacggac aaacctcacg ttccttaccg
      601 gccggcgacg gcccaaggac tgactgcaaa tgctacgaat gaacgatcag cggtatttcc
      661 tgttattctg tcgggcaaaa tcttatgcaa gatgttgcga ggtgtcaaca atttcttgcg
      721 tttattacca cagtcttctt tccttgcgat cggttgtcga tgtctgcatt tcccgtcaat
      781 gacggaacac cccaatacgg aacagcctcg ggagtcgggt cggagcagcg tccgcttccg
      841 aaagtcgaat ccagcgcgct ggcggcgcgt ttgcaggcga ttatcggcaa ggagtccgtg
      901 tcgtcctttg cgcgccggtg cggactcgcg gaatcggtac tgcggaccta tctcagggac
      961 ggtcgcatgc ctccgctcga taaggcctgc gcaatggcag cggccgccgg tgtctcggtc
     1021 gactggctgg ctaccggcaa cggcccgggc agggctgcag aagtgcgggc aagctaccat
     1081 tcaggtgcgc cgggtgtggt cgcctccgac acgcggccgg tcgacaccgg tgtgctggaa
     1141 ggaattctca aggctgtcct ggaagcacag gacacacacg ccccaccagc gtatctggcc
     1201 gcactgattg ccgatcttta ccggcgggca atggatcggc acctaccggc atagttcgca
     1261 ggcggctatt gctgctgtct tgtggttgca cgccgcggcc ataccccgga acggggcgca
     1321 ggcagttccg gagtgttcga cgatctccac tgcaagcgct tcgggtcgcg gcagatcgtc
     1381 gagatttccc aggctgtcgt ccctgggtca gatactgtcc aattcggtgc cacgattacg
     1441 accatgaaca atacttcgtc cttttctcgc gactctcggg cagcctggct gttggcgggg
     1501 gtcgagctgt ttttctccct gagctgggtg gtctacgtca ttttcctgcc cgaactgttg
     1561 ctgcggggtg gggtggataa gcactatctg ccgtggctcc tggcggtcga ccagttgctg
     1621 ttcgcgctgg ccgactggtc gatgggtgtg gcggtagatc gcgctcgcgc ggcgctgcgc
     1681 aggattgggc cgatgctggt gctgctgtcg gcagtttcgg cgctggccat gttgctgttg
     1741 ccgtggctgt cagggatgcc gatgcttttc ctgttgatga ccggcttgtg ggtggcaact
     1801 tcctcggccc tgcgcgcgcc accttacgtg ctgctttcgc gctatgccgg gcgctcctcg
     1861 gtggccggtc tggccggcat ccagttgct
//
LOCUS       DAOSIT010000490         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_52908_length_1888_cov_8.897981, whole genome shotgun sequence.
ACCESSION   DAOSIT010000490 DAOSIT010000000
VERSION     DAOSIT010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52908_length_1888_cov_8.897981"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1888)
                     /locus_tag="PLL14_10305"
     CDS             complement(<1..>1888)
                     /locus_tag="PLL14_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOG04363.1"
                     /translation="PQHWTVEPTADGERQFVIRRKGEVQLVTLAYRTPSSLHADSDAI
                     GMATEVLGDTPNGRLYRALVQPGLATQVFAYTMDTREPGFVVFGAMVNKGDALDRVRE
                     RMIEVIEGGFAREAATAAELARAVEQERLGYERMLADPEAFAVTLSEYIALGDWRLFF
                     HARDQLDQIKPEQLDAAASKYFVRDNRVLGTFIPDESPRRALVPPTPSPAQVLASFKP
                     QEKGDAGEVFDPSYDNLDSRTRLRTFGDLKVALLPKKNRGQTVNVAMTFRWGDESSLR
                     DRNMVGTLAIAMLARGTRQLTRQQIADELTRLKVRGRLTHFETTRDKLPEALRLVAQM
                     LQDASFPPEEFAELKREYLTALQAQLDSPEELSSDALNSHFNTYPPGDPRYYSSLAER
                     IEQLRKITLEQVVAYHRELIGTARGQIAIVGDFDEQAIEEEIARVFPIRVSGSPYARV
                     DREFRRIAPQRMVIDTPDKENAFLRARLDINLRDDDPDAAALQVANTIFGGHSGLSSR
                     LLDRLRQKEGFSYSATSSLAMGSRQRLANWTVVAMVAPQNAARAEQAFLEELQRARRD
                     GFTATEVAEAKKGVLEARTVTRSQDSEVAARWASYLDLGRNWQFSKDFEARVMALTPE
                     QVNAA"
BASE COUNT          319 a          596 c          616 g          357 t
ORIGIN      
        1 gcggcgttaa cctgctcagg ggtgagcgcc atcaccctgg cctcgaagtc cttcgagaat
       61 tgccagttgc ggccaaggtc gagatacgat gcccagcgcg ccgcgacttc gctgtcctgc
      121 gagcgggtca cggttcgcgc ttcgagaacg cctttcttgg cctccgcgac ctccgtggcc
      181 gtgaagccgt cacgccgtgc gcgctgcagt tcctccagga aggcctgttc cgcgcgggca
      241 gcgttctggg gcgccaccat ggccacgacc gtccagttcg ccagccgctg gcgactgccc
      301 atggccagag aggaggtggc actgtagctg aaaccctctt tctggcgcag gcggtccagc
      361 agccggctcg atagtccgga gtggccaccg aaaatggtat tcgccacctg caacgcagca
      421 gcatcgggat cgtcatcgcg cagattgatg tcgaggcgtg cgcgcagaaa tgcgttttcc
      481 ttgtccggcg tgtcaatcac catgcgctgc ggcgcaatgc ggcgaaactc gcgatcaacg
      541 cgcgcgtaag gcgatcctga gacgcgaatc ggaaacactc gggcaatttc ctcctcgatc
      601 gcctgctcgt caaaatcacc gacgatggcg atctggccgc gtgccgttcc gatcagttcg
      661 cggtgataag caacgacctg ctccagggtg atcttgcgga gctgttcgat gcgctcggcc
      721 aggctgctgt agtagcgggg atcaccgggc ggataggtgt tgaaatgcga attcagtgcg
      781 tcgctcgaca gttcctcggg gctgtcaagc tgcgcctgga gggcggtcag gtactcccgc
      841 ttgagttcgg cgaattcttc cggcggaaag ctggcgtcct gaagcatctg cgccaccagc
      901 ctgagcgcct cgggaagctt gtcgcgggtg gtctcgaagt gggtcagacg gcccctgacc
      961 ttcaggcggg tgagttcgtc ggcaatctgt tggcgggtca actggcgggt accgcgagcg
     1021 agcatggcga tcgccagcgt gccgaccatg ttgcgatccc gcagcgagct ttcatcaccc
     1081 cagcggaagg tcatggccac atttaccgtc tgaccccggt tcttcttggg cagcagcgcc
     1141 actttcagat cgccgaaggt ccgcagccgg gtacggctgt cgagattgtc gtaggagggg
     1201 tcgaatacct cgccggcatc ccctttctcc tggggtttga agctggccag cacctgcgcc
     1261 gggctcggcg tcggcggcac cagcgcgcgc cgcggggact cgtcggggat gaaggtaccg
     1321 agcacacggt tgtcgcgtac gaagtattta ctggcggctg cgtccagttg ttccggtttg
     1381 atctggtcga gttgatcgcg ggcgtggaaa aacagccgcc agtcgccgag tgcgatgtac
     1441 tcggagagcg tcacggcaaa ggcctcgggg tcggccagca ttcgctcgta acccaggcgc
     1501 tcctgctcga ccgcacgagc gagttcggcg gcggtcgcgg cctcacgcgc gaaacctcct
     1561 tcgatcacct cgatcatgcg ctcacgaacg cgatccagcg cgtcgccctt attgaccatc
     1621 gcgccgaaga ccacaaaacc gggctcacgc gtgtccatgg tgtaggcaaa aacctgcgtg
     1681 gccaggcccg gctggaccag cgccctgtag agacgaccgt ttggcgtgtc gcccagcact
     1741 tccgtggcca tgccgatggc gtcggaatcg gcatgcaggc tcgacggcgt ccgataggcc
     1801 agggtcacga gttggacctc gcccttgcgc ctgatcacga actgacgctc gccatcggcc
     1861 gtgggctcga cggtccagtg ctgcggca
//
LOCUS       DAOSIT010000491         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53015_length_1885_cov_11.391257, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000491 DAOSIT010000000
VERSION     DAOSIT010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53015_length_1885_cov_11.391257"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLL14_10310"
     CDS             <1..595
                     /locus_tag="PLL14_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG04364.1"
                     /translation="TAQAVVDALPHPITLCGWSLGAMLAMRAALLAPQRVTGLVLVGA
                     TARFTQRADWPEAQAPALLDSFSDSIARQPEQTLQRFVALLCQGDHQARSLTRTLRAG
                     LRNAPVPDSKVLNRGLHWLREVDLRALLPALSTRSLLIHGENDALNPLAAARRLAGLL
                     PDARLEVFAGAGHAPFLADPQRFIQLLDDFRDAHTIS"
     gene            576..1346
                     /gene="bioC"
                     /locus_tag="PLL14_10315"
     CDS             576..1346
                     /gene="bioC"
                     /locus_tag="PLL14_10315"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HOG04365.1"
                     /translation="MPTPSARQRVRESFDRAAASYDQAAVLQRQVGDDLLREFASNPE
                     PASILDAGCGTGYGLQILHRRWPTAGLTAVDFAPAMLALARPDADLCLAANIEALPCR
                     DACFTTWWSNLTVQWCDVDKVCREARRVLRPGGQLALSTLGPETFQELREAFTGIDRH
                     RHTLSFSEPATIAQALRDAGFADIRLIRQTISLPYPDLKSLLRAVKAIGANSVTEGAR
                     SGLLGRAAWQRVQAAYERHRTSAGLPARYDVILAYARK"
     gene            1343..>1885
                     /gene="bioD"
                     /locus_tag="PLL14_10320"
     CDS             1343..>1885
                     /gene="bioD"
                     /locus_tag="PLL14_10320"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOG04366.1"
                     /translation="MNDRRGWFITGTDTEIGKTFVACALLHAFRRTGMAALAMKPVAA
                     GVDAHGLNEDVERLLAASSMQPTRTLVNPYGFRAAIAPHIAAAEEGRRIELPQIAAAL
                     STLLTMADVVLVEGVGGFCVPLGDTRDTADLASLLALPVILVVGMRLGCINHALLTQQ
                     AIAARGLPLAGWVANRIDPAM"
BASE COUNT          277 a          695 c          608 g          305 t
ORIGIN      
        1 aacggcacag gccgtggtcg acgccctgcc gcatcccatc accctctgtg gctggtcgct
       61 cggcgccatg ctggcgatgc gcgccgcctt gctcgcgccg cagcgcgtca ccggtctggt
      121 gctggtcggc gccaccgccc gtttcacgca gcgcgcggat tggccggagg cacaggcgcc
      181 ggccctgctc gacagttttt cggacagtat cgcgcggcag ccggagcaga ccttgcagcg
      241 cttcgtggcc ctgctctgcc agggcgacca ccaggcacga tcgctgacgc gtacgctgcg
      301 tgccggcctg cgcaacgccc ccgttccgga cagcaaggtg ttgaaccgcg gcctgcactg
      361 gctgcgcgag gtcgacctgc gtgccctgct gccggcactg agcacccgca gtctgctgat
      421 ccacggcgaa aatgacgccc tgaacccgct ggccgccgcc cggcgcttgg cgggactcct
      481 gcccgatgcc cgcctggagg tgttcgctgg agccggacac gcgccgttcc ttgccgaccc
      541 gcagcgcttc atccaactgc tcgacgactt ccgtgatgcc cacaccatca gctaggcagc
      601 gtgtgcgcga atccttcgat cgcgccgcgg cctcgtatga ccaggcggcg gtactgcagc
      661 ggcaggtcgg cgacgacctg ttgcgcgaat tcgcctccaa tcccgaaccg gccagcatcc
      721 tcgacgccgg ctgcggtacc ggctatggac tccagattct gcatcgccgc tggccgacgg
      781 cagggctcac cgcggtcgac ttcgctccgg ccatgctcgc gctggcacgc ccggatgccg
      841 acctctgcct ggcggcgaac atcgaggcgc taccctgccg ggacgcgtgc ttcaccacct
      901 ggtggtcaaa cctcacggta cagtggtgcg atgtcgacaa ggtgtgccgt gaagcgcggc
      961 gcgtcctgcg tcctggcgga caactggcgc tgagtacgct cggcccggaa accttccagg
     1021 aactgcgtga ggcctttacg ggtatcgatc gccaccgcca tacgctgtcg ttcagcgaac
     1081 cggcaacgat tgcccaggcg ctgcgcgacg ccggcttcgc cgacatccgg ctgattcgcc
     1141 agacgatcag cctgccctac cccgatctca agtcgctgct gcgcgcggtc aaggcgatcg
     1201 gcgccaactc ggtcaccgaa ggcgcacgca gcggtctgct cgggcgcgcg gcgtggcagc
     1261 gcgtgcaggc ggcttacgaa cggcaccgca catcggccgg gctgcccgcc cgttacgacg
     1321 tgattctcgc ctacgcccgc aaatgaacga ccgacgcggc tggttcatca ccggcaccga
     1381 taccgaaatc ggcaagactt tcgtcgcctg cgcgctgctg cacgcctttc gtcgcaccgg
     1441 catggcggcg ctggcgatga aaccggtcgc cgccggcgtc gatgcgcatg gcctcaacga
     1501 ggatgtcgag cgcctgctcg cggcatcgtc gatgcagccg acacggaccc tggtcaatcc
     1561 ctacggtttc cgtgccgcga ttgcaccgca catcgccgcc gcggaagaag gacggcgcat
     1621 cgaactgccg cagatcgccg ccgcgctgag cacgctgctg accatggccg acgtcgtcct
     1681 ggtcgaaggg gtcggcggtt tctgcgtgcc gctcggcgac acccgcgaca ccgccgacct
     1741 ggcgtcgctg ctcgccctgc cggtgatcct ggtcgtcggc atgcgcctgg gctgcatcaa
     1801 ccatgcgctg ctgacccaac aggcgatcgc cgcccgcggc ctgccgctgg ccggctgggt
     1861 ggccaaccgc atcgatccgg cgatg
//
LOCUS       DAOSIT010000492         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53228_length_1880_cov_7.160000, whole genome shotgun sequence.
ACCESSION   DAOSIT010000492 DAOSIT010000000
VERSION     DAOSIT010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53228_length_1880_cov_7.160000"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..352
                     /locus_tag="PLL14_10325"
     CDS             152..352
                     /locus_tag="PLL14_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04367.1"
                     /translation="MSHSHETTIPWHDSELMRVNGHPLLYTITKADGMLCRLPGGKSE
                     TVEGLRAKGFNVVMPKRVRIFD"
     gene            391..636
                     /locus_tag="PLL14_10330"
     CDS             391..636
                     /locus_tag="PLL14_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04368.1"
                     /translation="MSAPAKPLRQAMPRVAEFVDAAREAFGAAAVDQAIRNGLAGGTD
                     FHASEGGHTVGHLPAAPGASFTFDQLQITRKSKDAKP"
     gene            871..1617
                     /locus_tag="PLL14_10335"
     CDS             871..1617
                     /locus_tag="PLL14_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04369.1"
                     /translation="MIPLSVRLEGFDALAASLGDQARQIPFAASQALNTTARSVREAT
                     LAEMAAQFDRPTPLVMKSLFISPATKAKLQAAVYLKDREIGGKNSRSMAEILGHQFAG
                     GTRLRKRMENAFTEAGLISLGEYLVPGPDAKLDQYGNLSRGQTQQIYAALRLFRDPYQ
                     NATQSARSQRHAKAAGRIFWSDGKGANKRRRGLWATDARGFPKLLMLVIPTPVYRRRI
                     DMDRQSAVIVASDFSANFDAALKKALATAR"
     gene            1622..>1880
                     /locus_tag="PLL14_10340"
     CDS             1622..>1880
                     /locus_tag="PLL14_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04370.1"
                     /translation="MTTATLTELQTERARLKALDARRELDEATEQTRRAADLLRAALA
                     VRALLADVLRTVPARLAQAIEGEHDETRVHYLLSDAVHTLLD"
BASE COUNT          356 a          700 c          539 g          285 t
ORIGIN      
        1 gagcacgtct gttgatacgt gataccgatg gcagatcggg gcggggcggc ttcggccgcc
       61 acgataaccg cacctttcac agagccagcg cccggacctc tgaccaatct gcctatccgg
      121 gcaccttttc catttcagga ggcagcacgt tatgagccac tcgcacgaaa caactatccc
      181 atggcacgac agcgaattga tgcgagtgaa tgggcatcca ctgctttaca cgataaccaa
      241 ggccgatggg atgctgtgcc gactccccgg cgggaaatcg gaaaccgtcg aagggttgcg
      301 cgccaaaggg ttcaatgtcg tcatgccaaa acgtgtgcgc atcttcgact agcgggaatg
      361 cgccaccagc aaagccagcg ggccgctgcg atgagcgcgc cagccaaacc cctgcgccaa
      421 gccatgccgc gcgttgccga attcgtcgac gccgcccgcg aagccttcgg cgccgcggcc
      481 gtcgatcaag ccatccgcaa cggcctggcc ggcggcaccg acttccacgc cagcgaaggc
      541 ggtcataccg tcggccacct tccggccgct cctggcgcca gcttcacctt cgaccaactg
      601 caaatcaccc gcaagagcaa ggacgccaaa ccatgatccc cgccgccacc gtaatccacg
      661 ccgaacctcc gttctcctgc gccgggtgcc ggtatctgtc cgaggctcgc cgatcgttag
      721 caggatggtc ctcagtggga tacgtcagcg agttatgcac ggtcttctgc cgtctccacg
      781 tcaccccact tgcgcgctgc aggctctacg aacacccgcc gaccggcaat ccttcccacc
      841 tctccagcct gatgaccaga taggccagcc atgatccccc tatccgtgcg cctcgaaggc
      901 ttcgacgccc tggccgccag cctcggcgac caggcccgcc agatcccgtt cgccgcttcg
      961 caagcgctca acaccaccgc ccgctccgtg cgcgaggcca cgctcgccga aatggccgcg
     1021 cagttcgacc gcccgacgcc gctggttatg aaaagcctgt tcatatcccc cgccacaaag
     1081 gccaagctgc aagccgccgt gtacctcaag gatcgcgaga tcggcggcaa gaactcccgc
     1141 tccatggccg aaatcctcgg gcaccagttc gccggcggaa cgcgcctgcg caagcgcatg
     1201 gaaaacgcct tcaccgaggc cggcctgatc agcctcggcg aatacctggt gcccggcccg
     1261 gatgcaaagc tcgaccagta cggcaacctc tcgcgtggcc agacgcagca gatttacgcc
     1321 gccctgcgcc tcttccgcga cccctaccag aacgccacgc agagcgcgcg cagccagcgc
     1381 cacgccaagg ccgccgggcg cattttctgg tcggatggca aaggcgccaa caagcgccgg
     1441 cgcggcctgt gggccaccga cgcgcgcggc tttcccaagc tgctgatgct ggtgattccg
     1501 acgccggtct atcggcggag aatcgacatg gatcgccagt ctgcggtgat cgtcgcgagc
     1561 gacttcagcg ccaacttcga cgccgcgttg aaaaaggctc tggcgacggc tcgctgacga
     1621 cgtgaccacc gccaccctca ccgaactcca aaccgagcgc gcgcgcctca aggcgctcga
     1681 cgctcgccgg gagcttgacg aggctacgga acagactcgg cgcgccgccg acctgctgcg
     1741 cgccgcgctt gccgtgcgcg cgctgctggc cgacgtcctg cgcaccgtcc ccgcgcggct
     1801 cgcgcaagcc atcgaaggcg agcacgacga gacccgggtg cactacctgc tttccgacgc
     1861 cgtgcatacc ctgctcgacg
//
LOCUS       DAOSIT010000493         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53386_length_1876_cov_9.573311, whole genome shotgun sequence.
ACCESSION   DAOSIT010000493 DAOSIT010000000
VERSION     DAOSIT010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53386_length_1876_cov_9.573311"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1843)
                     /locus_tag="PLL14_10345"
     CDS             complement(14..1843)
                     /locus_tag="PLL14_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOG04371.1"
                     /translation="MFIRRTRTNNQATGEGYFTYRLVRGERVGGKVRQITVLNLGRNF
                     AIKQEDWPTLCSRIEHLLHPQQALLPLQCAEPIERAAQRYAGQLVARAPAVEPAAADA
                     GNLRPLADFQEVDVDSLQLTQPRSVGVEQVGLFALTELGLIDTLSALGVSGVVRAAII
                     GNLIGRMGRPGSELATWNWLVRQSALGELIDVDFATMSHMSLYRASDVLMKHREEIET
                     RLFSSVQTLFDLAETVTLYDLTNTYFEGEVSGNRKARRGRSKEKRSDCPLVTLGLVLD
                     GSGFVRRSQTFAGNVSEGSTLTGMLAALDAPAGALVVMDAGIATEANITWLGEHGYRY
                     LVVRRGGTRQFDETCSVTIETAAEEPLHLQKEVSQDGKERLLHCHSPSRQLKEEAMHA
                     KASQRFEAGLQQISEGLNKPRCEKRHDKLLERVGRLKQKSGGASQHFTVDLATDDSGK
                     IVTGLTWQKTPVNGTMATHPGVYCLRTNELAWDEERLWRTYTMLTDLESVFRSLKSEL
                     GLRPVYHHKENRSDGHLFITVLAYQCVQFLRVKLKAAGITDSWATLRETLSVQRRVTA
                     TFRQRDGRTLNVRKATVAEPELMAIYRALGINPTPGGTKKLIT"
BASE COUNT          333 a          586 c          572 g          385 t
ORIGIN      
        1 cgtaatttcg gggtcaggtg atgagtttct tcgtccctcc tggtgtgggg ttgatgccca
       61 gggcgcgata gatggccatc aactcgggct cagcgacggt tgccttgcga acattgaggg
      121 tacggccatc gcgctggcgg aaggtggcgg tgacccggcg ctgcaccgag agggtctcac
      181 gcagggtcgc ccaactatcg gtgatccccg cggcctttaa ctttacccgc aggaactgta
      241 cgcactggta ggccagcacg gtgatgaaga gatggccgtc tgagcggttc tccttgtggt
      301 gatagaccgg tcgcaagccg agttcgctct tcagactacg aaagacgctc tcgaggtcgg
      361 tgagcatcgt ataggtgcgc cagagccgct cttcatccca cgcgagttcg ttggtgcgca
      421 agcaatagac ccccggatgg gtggccatcg tgccgttgac cggggttttc tgccaagtga
      481 gcccggtgac gatttttccg gagtcatcgg tggcaaggtc taccgtgaag tgctggctgg
      541 cgccgccgct tttctgcttg agtcggccga cgcgttcaag taacttgtcg tggcgtttct
      601 cgcaacgagg cttgttcagg ccctcgctga tttgctgcaa cccggcttcg aaacgctggc
      661 tggccttggc gtgcatcgcc tcttccttga gctggcgact cggagaatgg caatgcagga
      721 gccgttcttt gccgtcctgg ctgacctcct tctgcagatg cagtggctcc tcggcagccg
      781 tctcgatggt caccgagcag gtctcgtcga actggcgtgt gcccccgcga cgaaccacca
      841 gatagcggta cccgtgttcg ccgagccacg tgatgttggc ctcggtggcg atgcctgcgt
      901 ccatgaccac cagcgcccct gccggggcgt ccagtgcggc cagcatgccg gtcagtgtgc
      961 tgccttcact cacgttgccg gcgaaggtct gtgagcgccg cacaaagccg ctgccgtcaa
     1021 gcaccagacc gagggtcacc aacgggcagt cgctgcgctt ctctttcgaa cgcccgcgcc
     1081 tggccttgcg attgccactg acttcgcctt cgaaataggt gttggtcagg tcgtagaggg
     1141 tgaccgtctc tgccaggtca aagagcgtct gaacgctgct gaacagacgc gtttcaatct
     1201 cctcccgatg cttcatcagg acgtccgaag cgcggtacag gctcatgtgt gacatcgtgg
     1261 cgaagtcgac gtcgatcaac tcacccaggg cactctgccg caccagccag ttccaggtcg
     1321 ccagttcaga gccgggccgc cccattctcc cgatcagatt gccgataatc gccgcacgca
     1381 cgacgccact gacgcccaat gcgctcaacg tgtcgatcaa acccagttca gtcaaggcaa
     1441 agagtcccac ctgttccaca ccgaccgaac ggggttgtgt cagttgcagc gagtcgacat
     1501 cgacttcctg gaagtccgct aacggccgta aatttccagc gtcagcggcc gccggctcga
     1561 cggccggcgc gcgcgccacc agttgcccgg catagcgctg agcggcacgc tcgataggct
     1621 cagcgcattg cagcggcagc agcgcttgct gaggatgaag caggtgctcg atgcggctac
     1681 acagggtcgg ccagtcttcc tgcttgatcg cgaagtttcg cccgaggttc aggacagtga
     1741 tctgccgcac cttgcccccg acgcgctcgc cgcgcaccag ccgataggtg aagtacccct
     1801 cgccggtcgc ctggttgttg gtccgtgtcc gtcgaataaa catgctcacg atcgtacaac
     1861 aaaagcaagg gaaatt
//
LOCUS       DAOSIT010000494         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53471_length_1874_cov_13.603079, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000494 DAOSIT010000000
VERSION     DAOSIT010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53471_length_1874_cov_13.603079"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1805)
                     /locus_tag="PLL14_10350"
     CDS             complement(<1..1805)
                     /locus_tag="PLL14_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOG04372.1"
                     /translation="MTLAQHFAAYSEWRAQLSGVLDNFAAWLVENELSDAQTDRRITQ
                     LLEKLREDRLHVAFVAEFSRGKSELINAIFFAGYGNRILPSTAGRTTMCPTELMFDRS
                     KAPSIELLPIGSRESNASISEYKRFPEEWEVVPLATGSAESMQEALRHVSDVIRVDPA
                     TAEKLGFAIADGDNAVFRVDADGCVEIPRWRHAVINFPHPLLQQGLVILDTPGLNAIG
                     TEPELTLSLLPNAHAVLFILAADTGVTQSDLTVWREHIGNTGGRRKGRIVVLNKIDSL
                     WDELKSAEEIDAEITRQVDTCAWTLALPEDQVFPVSAQKALVAKINGDAELLKRSRLS
                     ELEFALSEELIPAKQEIVRDNTDAEFADCYQRARSLLESRLVNLREQVTELTELRGKN
                     KGVVEYMMGKVRVEKEEFESGLQRYYAVRSVFSQLTNNLFAHLGLDSLRLLTTTTREA
                     MVKATFSKTLSGAMAHFFDVARGNLARSEGEVTEILAMMEAIYKKFAVEHGLKLGSPI
                     GFSLLRYEKEIDRLEQWCDTHINTMFQLLTHEKSQLTQRFFEEVTVQVRKSFEHANRD
                     AESWLKAIMAPMEIQIREHQIQLKRRLESIKRIHQA"
BASE COUNT          364 a          552 c          554 g          404 t
ORIGIN      
        1 gcctggtgga tgcgcttgat gctttccagc cgacgtttga gctggatctg gtgctcgcgg
       61 atctgaattt ccatcggtgc catgatcgcc ttcagccacg attcggcgtc acggttggcg
      121 tgttcgaagc tcttgcgcac ctgtaccgtc acttcctcga agaagcgctg cgtcaactgg
      181 ctcttttcgt gtgtcagcaa ctggaacatg gtgttgatgt gggtatcgca ccattgctcg
      241 agacggtcga tttccttctc gtaacgcagc agcgaaaaac caatcggcga tccgagcttg
      301 aggccatgct caaccgcgaa ttttttgtag atggcttcca tcatggccag gatttcggtc
      361 acttcgccct cggatctcgc gaggttgcca cgcgcgacgt caaagaaatg cgccatcgct
      421 cccgacaagg tcttggagaa ggtggctttg accatcgcct cacgcgttgt cgtcgtcagc
      481 aggcgcagcg aatcgagccc gaggtgagca aagaggttgt ttgtcaactg cgagaaaaca
      541 ctgcgcacgg cgtagtagcg ctgcaggccg gattcgaatt cctccttttc aacccgcacc
      601 ttgcccatca tgtactcgac gacgcccttg ttcttgccgc gcagttcggt gagttcggtc
      661 acctgctcgc gcagattgac caggcgcgac tcaagcaggc tgcgcgcgcg ctggtagcaa
      721 tcggcaaact cggcgtcggt attgtcacga acgatttcct gtttggccgg gatcagttct
      781 tcggagagcg cgaactcaag ttccgacagg cggctgcgtt tcagcagttc ggcatcgccg
      841 ttgatcttgg caaccagcgc cttctgggcc gaaaccggga acacctggtc ttccggcagt
      901 gccagtgtcc aggcacaggt atcgacctgc ctggtaatct cggcgtcgat ttcctctgcc
      961 gacttgagtt cgtcccacag gctgtcgatc ttgttgagga cgacgatccg ccccttcctg
     1021 cggccaccgg tgttgccgat gtgttcgcgc cagacggtca gatcggactg ggtgacaccc
     1081 gtgtcggcgg ccagaatgaa gagtacggcg tgcgcgttcg gaagcagcga caaggtcagt
     1141 tcgggttccg tgccaatcgc gttcaaaccg ggcgtatcga gaatgaccag tccctgctgc
     1201 aaaagcggat gcgggaagtt gatgaccgca tgacgccagc gcggaatctc gacgcacccg
     1261 tcggcgtcta cgcgaaacac cgcgttatcg ccatcggcaa tcgcaaaacc aagcttctcg
     1321 gccgtcgccg ggtcgacacg tatgacatca ctgacgtgtc gaagcgcttc ctgcatggac
     1381 tcggccgaac ccgtggcgag cggcacgacc tcccattcct cgggaaagcg tttgtactcg
     1441 ctgatactgg cgttggattc gcggctaccg atcggcagca gttcgatcga cggtgccttg
     1501 gaacgatcga acatcagctc ggtcgggcac atcgtcgtgc gccccgcggt cgagggcagg
     1561 atccggttgc cgtaaccggc gaaaaatatg gcattgatca actcggactt gccgcgtgaa
     1621 aattcggcga cgaaggccac atgcagacga tcctcgcgca gtttctcgag cagctgcgtg
     1681 atgcgccggt cggtctgggc gtcgctcaac tcgttctcga cgagccaggc ggcgaaattg
     1741 tcaagcaccc cggagagctg agcacgccat tcgctgtagg cggcaaaatg ttgggcaagc
     1801 gtcatggcag gttccggcac aaggtacagg ttttcactct atcacaaaga ttcttccaat
     1861 cagcgacatg gacc
//
LOCUS       DAOSIT010000495         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53475_length_1874_cov_7.091809, whole genome shotgun sequence.
ACCESSION   DAOSIT010000495 DAOSIT010000000
VERSION     DAOSIT010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53475_length_1874_cov_7.091809"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..685
                     /locus_tag="PLL14_10355"
     CDS             122..685
                     /locus_tag="PLL14_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOG04373.1"
                     /translation="MYTLIRKLAVAATMSVAALAAQAAPVTITFNEAGAPLGPLDGTT
                     FYQSYGIAGFTQAVRFGFDGRLPDDGFGIYNNGFSGTVLFDEDMSNVSMTWAVSGSGV
                     TFHAEAYDLNNVLVADFSSGGVGLSGLASFAASNVRSIVFHDGGAQVAIDTLNFTRSG
                     TVPEPISLALVGMGLVAAGAARRRKQI"
     gene            1148..1384
                     /locus_tag="PLL14_10360"
                     /pseudo
     CDS             1148..1384
                     /locus_tag="PLL14_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031943491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          338 a          542 c          605 g          389 t
ORIGIN      
        1 tactgtcgag aaattttaca gaacctgccg aagcgcaggg cgaattatgc taaccaattg
       61 attgtattgt tgtggcacgc gatttgcttt gaaggttgtg ttactcattc tggaggttcc
      121 tatgtacaca ttgatccgca agctggcagt cgccgccacg atgtctgtcg ccgcattggc
      181 ggcgcaggct gcaccggtca ccatcacctt caacgaggcc ggtgcacctc ttggcccgct
      241 cgacggcacc accttctacc agtcctacgg cattgccggg ttcacgcagg ccgttcgctt
      301 cggctttgac ggccggctgc ccgacgacgg cttcggcatc tataacaacg gtttcagtgg
      361 gaccgtgctg ttcgacgaag acatgtccaa cgtcagcatg acttgggccg tctctgggag
      421 tggtgtcacc ttccacgccg aagcgtacga cctgaacaac gtgctggtgg ccgacttcag
      481 ttccggtggt gtcggtcttt ccggcctggc ctcgttcgcg gccagcaacg tgcgcagcat
      541 cgtcttccac gacggcggtg cccaggttgc gatcgacacc ctgaacttca cccgcagcgg
      601 cacggtgccc gagccgattt ccttggccct cgtcggtatg ggcctggtgg ctgcaggcgc
      661 tgcccgtcgc cgcaagcaga tctgagtttc tgaacgcgag caaagccccg ccaggcgggg
      721 cttttttatg tgcgccctgc acggcacggg cgcactgcac gaagggtagg aaatccaacg
      781 gcactatcga gagtatatga gccttcgtca aggggcgcca atctcgaatt tcgcccggtg
      841 gcccttgaaa cttgtcgaga tcgcgaagac ggctcagcac ctcggttatt ccgaagtacg
      901 cttttcaacc gagcctcttg caaaatcttg caaaactcgc tattcagccg aatttttcag
      961 tgcaagcggt taggaagggg tggcaggtcg ccatttctgg catgattgga gcgtcgaaac
     1021 cctgatcacg cctgccatga ggaacgatag cacaggcaca gcttgccggc tgatggaccg
     1081 gtggaatgtg gtgcagcgtg aactgatccc cagaacttga ggcgccctta cgccgaagca
     1141 ggagcgggtg atccacattc tcgagtgggt gcggatcgag gagttcacgg cagtgtcgtg
     1201 gtgtggtgtc gggtgtccgc cctttgagcg agcttggttg gcgaacgcct ttgtggccaa
     1261 ggcggtgttg gggttggcga cgacggtggg gctgattgag cggctgacga tcgaccgtgc
     1321 gttgcgccgc atctgcggtt tcccgcgggg caagagactg ccttccgaaa ccaccttctc
     1381 gcgcaccttg atcgtgccga tcagcaaacg acggaagcgg gcgatgaccg actgtgcctg
     1441 ccggttggtc agggcattgt cggcgctgta cgcctggctg gggttcaccc ggcggacctc
     1501 aaaccgggga tgcggctggt cgtgggttcc cgcttggtcg gcacggaggt cgacacccga
     1561 accagggcgt aggcttcggc tgtcggcagg aaaggtaaac gcatcgcacg ggctcaagaa
     1621 atgtgtcgtg cgcatcatag ccgcgctctg cgcttttgtg gtggaatgag ccaaggtcgc
     1681 gggcggcaag cggctgttcc cggcgaattc agactcatcc agcgcactcg acaccgcggc
     1741 gcgtggtgct ccgctgttgg cgcagtacaa cgcaaaaagc cccgccgtag cggggcttcc
     1801 agggaaatgc gggatctgct tactgcttgc ggcggcgcga tgcggcgagg ccggcgaggc
     1861 cgagaccgag cagg
//
LOCUS       DAOSIT010000496         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_53478_length_1874_cov_5.882903, whole genome shotgun sequence.
ACCESSION   DAOSIT010000496 DAOSIT010000000
VERSION     DAOSIT010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53478_length_1874_cov_5.882903"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLL14_10365"
     CDS             complement(<1..610)
                     /locus_tag="PLL14_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein E"
                     /protein_id="HOG04374.1"
                     /translation="MNAPSLHPHSRPLGQILISKGILSEDQLRIALLEQMKSNQAIGK
                     LLVALGFVSEATLRDALSESLGKQSVDLSNAIIDPSALKLVPRDLAKRHHVLPLDYDA
                     ENLRLTVAIADINDIVALDRIRGLTGDEIEIDTLLAGETEIDRAIDQCYGHELSIDGI
                     LHEIETGEIDFRGLQSSADEYSQPVVRLIDSILTDAVKHDSSD"
     gene            complement(662..>1874)
                     /locus_tag="PLL14_10370"
     CDS             complement(662..>1874)
                     /locus_tag="PLL14_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG04375.1"
                     /translation="RAEEAKRLAGPAEKPEIASAPLELKLDPLEPASSASRPLPPHAR
                     RPDPFGTQTTSETGPTPARPGVPLPGEMPADSSGSDAGQRSAAKNLFAAKQASRSTPR
                     SLWIFVGLGGFATLAIGGYFWWQLQSLSGGTLARPPVTALPAGQTPAPLVPAVQRPTP
                     PLPAPPVAAPVESPPPATAVRTPATTPRAGLPQSEFAGVPGGRAAGVFRQGGGREKQQ
                     QALDLAYQAWQADRLDDARHSYEQVLRSDARNADALLGLAAIAVRQGQPERAHDLYQQ
                     VLESDPNDVTAQAALINLRGQGDAGQSESRLKTLLASQADSSAVPFALGNVYARQGRW
                     SEAQQAYFQAYALEPDNADLLFNLAVSLDHLRQHKLAVQYYRMALSAAETRRGAFDRN
                     AAGKRILELQP"
BASE COUNT          306 a          590 c          643 g          335 t
ORIGIN      
        1 tatcggaaga atcgtgcttg acggcgtcgg tcaggatcga gtcgatcagg cgaaccaccg
       61 gctggctgta ctcgtcggcg gacgactgca ggccgcgaaa atcaatctcg ccggtctcta
      121 tctcgtgcag gatgccgtcg atcgacagtt catggccgta gcactggtcg atcgcgcgat
      181 cgatctcggt ttcgccggcg agcagggtgt cgatctcgat ttcgtcgccg gtcaggccgc
      241 gaatcctgtc cagcgcgacg atgtcgttga tatcggcgat ggccaccgtc aggcgcaggt
      301 tttccgcgtc gtagtcgagc ggcagcacat ggtggcgctt ggcgagatcg cgcgggacga
      361 gtttcagcgc cgacggatcg atgatggcat tcgacagatc gacgctctgt ttgccgaggc
      421 tttccgagag cgcgtcgcgc agcgtcgctt ccgagacgaa gccgagcgcg accagcaact
      481 tcccgatcgc ctgattcgac ttcatctgct cgagcaaggc gatgcgcagt tgatcctcgc
      541 tgaggatgcc cttgctgatc aggatctggc ccagcggtcg cgaatgcgga tggaggctgg
      601 gagcgttcat gggcggaaaa tccggcaatc cggatgcaga aatccctgca aggtgccgga
      661 attacggttg cagttcaaga atgcgcttcc cggcagcgtt cctgtcgaag gcgccacgcc
      721 gcgtttcggc ggcactcagc gccatccggt agtactgcac cgccagtttg tgctgacgaa
      781 ggtgatcaag gctgacggcc aggttgaaga ggagatcggc gttgtcgggt tccagcgcat
      841 aggcctgaaa ataagcctgc tgcgcctcgc tccagcggcc ctgccgggca tagacgttgc
      901 ccagcgcaaa cgggacggcg gacgaatcgg cctggctggc cagcagggtc ttcaaacggc
      961 tctcggactg gcccgcatcg ccctgcccgc gcaggttgat caaggctgcc tgcgcggtga
     1021 cgtcgttggg gtcggattca agcacctgct gatacaggtc atgcgcacgc tctggctgcc
     1081 cctggcggac agcaatcgcc gccagaccga gtagcgcgtc ggcattcctg gcgtcgctgc
     1141 gcaggacctg ctcatagctg tggcgcgcat cgtccagccg atcggcctgc cacgcctggt
     1201 aggcgaggtc gagcgcctgc tgctgctttt cccggccgcc gccctgacga aaaacccccg
     1261 ctgcgcggcc acccggaaca ccggcgaact cactttgcgg taaccccgca cgcggcgttg
     1321 tggcaggtgt cctgacggct gtcgccggtg gcggcgactc caccggcgcc gcgaccggcg
     1381 gcgccggcaa cgggggagtc ggacgctgaa cggccggcac caggggtgcg ggggtctggc
     1441 ctgccggcaa tgccgtgacc ggcggtctgg cgagcgtgcc accggacagc gactgcaact
     1501 gccaccagaa ataaccgccg atggccagtg ttgcgaaacc tcccagaccc acaaagatcc
     1561 acagtgatcg gggcgtcgaa cgggaagcct gtttggcggc aaagaggttt ttcgccgcgc
     1621 tgcgttggcc ggcatcgctg ccgctggaat ccgccggcat ttctcccggc agcgggacgc
     1681 cgggtcgggc cggcgtcggg cccgtttccg atgtcgtctg ggtgccgaac gggtcggggc
     1741 gcctggcgtg cggtggcagt ggccggctcg cggaactcgc cggttcgagc gggtcgagct
     1801 tcagttcgag tggagcagac gcgatctcgg gcttctccgc tggaccagca agccgtttgg
     1861 cctcctcggc ccgg
//
LOCUS       DAOSIT010000497         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54081_length_1859_cov_11.053769, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000497 DAOSIT010000000
VERSION     DAOSIT010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54081_length_1859_cov_11.053769"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1859)
                     /gene="glyS"
                     /locus_tag="PLL14_10375"
     CDS             complement(57..>1859)
                     /gene="glyS"
                     /locus_tag="PLL14_10375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOG04376.1"
                     /translation="ALEGFMRAAGVSFAQLHKTTDGKSEYFVARREQPGEALDKHLSE
                     IVAHALRKLPIAKVMRWGDSEHQFVRPVHGLIMLHGTRVVPGEVLGLQSGRTTLGHRF
                     LSTGEISIDSPADYAPRLLAGKVIAAFPERRASIAAQLAAAAGKLAARINPADGLLDE
                     VTALVEWPAVYVGEFEPEYLEVPQECLILTMQQNQKYFPLLDSAGKLLNKFLIVSNMQ
                     VDDPRHIIAGNERVVRPRLSDARFFYNQDRKNGFATRVVRLGAVVYHHKLGSLGDRAQ
                     RLGRIARSIAEKLGADANLAERAALFAKIDLLTDMVGEFPELQGIMGRYYLLHEGGLP
                     VVADAIEQHYRPRFAGDTLPAGNIACAAALADKLDALVGFFGIGQLPTGDKDPFGLRR
                     AALGVLRILMETPLPLDLGELIGDAKAALRQQGIKLEEIDEPLLVFMLERLRGMLKDS
                     GHAVDVIEAVLAQRPTRIDRVPARLAAVREFLALPDSLALAAANKRIGNILRKAESVL
                     PEPDVALLEEDAEKALFQRVLLIAPVVRSHVANEDYADALKVLASARAEVDRFFDEVM
                     VNVQEPLLRGNRLGLLKVLFDQFNAVADISRLAV"
BASE COUNT          315 a          591 c          625 g          328 t
ORIGIN      
        1 gatgaactgc ttcgagtcgt agttgatgac gccgtcgcgg tcgagaatga cgagcttcat
       61 acggccagcc tggagatgtc ggcgaccgcg ttgaactggt cgaacagcac tttcagaagc
      121 cccagacggt tgccccggag caacggctcc tggacgttga ccatcacttc gtcgaagaag
      181 cggtcgacct cggcacgtgc cgaggccagc accttgagtg cgtcggcgta atcctcgttg
      241 gcgacgtgcg acctgaccac tggcgcgatc aaaagcacac gctggaacag ggctttctcg
      301 gcgtcttcct cgagcagggc gacgtccggc tcggggagta ccgactcggc cttcctgagg
      361 atgttgccga tccgcttgtt cgccgcagcc agcgccagcg aatcggggag cgccaggaac
      421 tcgcgcaccg ccgccaacct ggccggaacc cggtcgatac gcgtcggccg ttgcgcgagc
      481 acggcctcga tgacgtcgac cgcatggccg ctgtccttga gcatgccgcg caggcgctcg
      541 agcatgaaca ccagcagcgg ttcatcgatc tcttcgagct tgatgccctg ctgccgcagc
      601 gccgccttgg cgtcgccgat cagttcgccg aggtcgagcg gcagcggtgt ctccatcagg
      661 atgcgcagga cgccgagtgc cgcacggcgc aggccgaagg gatccttgtc gccggtcggc
      721 agttgcccga tgccgaagaa tccgaccagg gcgtcgagtt tgtcggccag cgcagccgca
      781 caggcgatgt tgcctgcggg cagggtgtcg ccggcaaagc gcggtcgata gtgttgttcg
      841 atggcgtcgg cgaccaccgg caagccgcct tcgtgcagca ggtagtagcg gcccatgatg
      901 ccctgcagtt cggggaactc gccgaccatg tcggtcagca ggtcgatctt ggcaaacagg
      961 gccgcgcgct cggcgaggtt ggcgtcggct ccgagtttct cggcgatgct gcgcgcgatc
     1021 cggcccagtc gctgcgcgcg gtcgcccagc gatccgagct tgtggtggta gaccaccgca
     1081 ccgaggcgaa cgacgcgtgt ggcaaagccg ttcttgcggt cctggttgta gaagaagcgc
     1141 gcgtccgaga ggcgtggacg cacgacgcgc tcgttgccgg caatgatatg acgcgggtcg
     1201 tcgacctgca tgttggaaac gatcaggaac ttgttgagca gttttccggc gctgtcgagc
     1261 agcggaaaat acttctggtt ctgctgcatc gtcaggatca ggcattcctg cggcacttcc
     1321 agatattcgg gctcgaactc gccgacatag accgccggcc attcgaccag cgccgtcacc
     1381 tcgtcgagca ggccatcagc cgggttgatg cgggccgcga gtttcccggc ggcggccgcg
     1441 agttgcgcgg caatcgacgc gcgtcgctcg gggaaggcgg cgatcacctt gcccgccagc
     1501 agccgcggtg catagtcggc agggctgtcg atgctgatct cgcccgtcga aaggaaacgg
     1561 tggccgagcg tcgtccggcc actctgcagg cccagcacct cgccggggac gacgcgcgta
     1621 ccatgcagca tgatcaggcc atgcaccggg cgaacgaact gatgttccga atcgccccag
     1681 cgcatgactt tcgcgatcgg cagcttgcgg agtgcgtgcg caacgatctc ggacagatgc
     1741 ttgtcgagtg cctcgcccgg ctgctcgcgg cgcgcgacaa agtactcgct cttgccgtcg
     1801 gtggttttat gcagttgcgc aaagctgacg cccgccgcgc gcatgaagcc ttcgagggc
//
LOCUS       DAOSIT010000498         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54082_length_1859_cov_9.959534, whole genome shotgun sequence.
ACCESSION   DAOSIT010000498 DAOSIT010000000
VERSION     DAOSIT010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54082_length_1859_cov_9.959534"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLL14_10380"
     CDS             <1..466
                     /locus_tag="PLL14_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04377.1"
                     /translation="QSPLTRTTYFDPGTDAKAARVLESCRTHERKVRLILGDTSTGEP
                     WLEEHDVIGRIGRSTGSLKVPLLIEPGEHGGCAILCACLLAIVDWESGDFLYRHAAYR
                     EADLSIKPSGDADRSWNVLRREEVVASFRDIGKAGAYLAFMRGATIEPRVFQ"
     gene            complement(548..763)
                     /locus_tag="PLL14_10385"
     CDS             complement(548..763)
                     /locus_tag="PLL14_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04378.1"
                     /translation="MIRLGSQIRLTRREVERFRKITDIEPVDIRTLDDLDAYIARCKA
                     HYWGVSKDTQFLHWLIDREYAQCRLAA"
     gene            1227..1808
                     /gene="radC"
                     /locus_tag="PLL14_10390"
     CDS             1227..1808
                     /gene="radC"
                     /locus_tag="PLL14_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG04379.1"
                     /translation="MNLSALSRADLVRELLSPMVCASSTNPLHVSDAVAQMGMLGTTD
                     TLLAHRLGIARELLLRDLREQMRSGPLMTSPQVLRDWLRLYCAGLEHEVFLALYLDAQ
                     HRLIEVEELFRGTLTQTSVYPRELVKGALARNAAALAVAHNHPSGEAEPSRADELLTQ
                     SLKQALSLVDVRVIDHFVVAGDTVVSFAERGLV"
BASE COUNT          316 a          606 c          571 g          366 t
ORIGIN      
        1 ccagtcgccc ctgacgcgca cgacctactt cgatcccggc acggatgcca aggccgccag
       61 ggtcctggaa tcctgccgca cgcacgaacg gaaggtccgc ctgatcctcg gtgataccag
      121 caccggggaa ccctggctgg aggaacacga tgtcatcggc cggatcggcc gctcgacggg
      181 gagtttgaag gtccccttgc tgatcgagcc cggcgagcat ggcggctgcg ccatcctgtg
      241 cgcttgcctc cttgccatcg tggattggga atcgggcgac ttcctgtatc gccatgccgc
      301 ctaccgcgaa gccgacttga gcatcaagcc ttcgggcgac gccgaccggt cctggaatgt
      361 tctgcggcgc gaagaagtcg tcgccagttt ccgggacatc ggcaaggccg gcgcctacct
      421 cgccttcatg cgcggcgcga cgatcgagcc gcgtgtcttc cagtgacttc gagggcagta
      481 cccgatggag caacgcctcc ccggcagaaa cgctggggag gcggtcgccc gcagaaaacc
      541 aaccgcgcta cgccgccagg cggcactggg cgtattcgcg gtcgatgagc cagtgcagga
      601 actgggtgtc cttggagacg ccccagtagt gcgccttgca tcgggcgatg taggcgtcca
      661 ggtcgtcgag cgtgcggatg tccaccggct cgatgtcggt gatcttccgg aagcgctcga
      721 cctcccggcg agtgagtcga atttgcgagc cgaggcggat catcgtcgtt ctacgtgaag
      781 ggttgagcct tcatcttgat caaatttttc aggcttgtcg cgggttttac ggcggtcccg
      841 caaaaaattt atgcagtgcc aataggctga catcccggtc acactggagt gcaagtaggc
      901 ccttttgtcc aagccggcgg caccgatccg cagcacgaag cgatcgccac gccgtcatca
      961 ccaacgccat tcgcaagccc gtcggattcc ggccgcttga acgttgcgtc ctctgcatcc
     1021 ccttgaatcc accctactga tgccggaaac tgactgggca tacctgcatt ccatcgtgga
     1081 atgtagtccg gccccgagcc ggcgtcgcag tcgatacctg cctcattcat cgtgccgacc
     1141 caggtccgca cattcatccc aagcggggac ctcgtcccct ttgggatcgg cgttcccgct
     1201 tttcttgttt cctagaggag aacgccatga atctatctgc cttgtcccgc gccgatctgg
     1261 tccgcgaatt actttcgccc atggtttgcg cgtcgagtac caaccctttg cacgtcagcg
     1321 atgccgtggc gcaaatgggc atgctcggca ccaccgatac ccttctcgcg catcgcctgg
     1381 gcattgcccg tgaactgctg ctgcgtgacc tgcgtgagca gatgcgtagc ggcccgctga
     1441 tgacctcacc gcaagtcctg cgcgattggc tgcggctgta ttgcgctggc cttgagcatg
     1501 aggtgttcct ggcgctctac ctggacgccc agcatcgcct gatcgaagtc gaggagttgt
     1561 tccgggggac attgacccag acctccgtct atcctcggga actggtcaag ggggcgctcg
     1621 cccgtaacgc cgccgcgctg gccgttgcgc acaaccatcc ctcgggggaa gccgagccct
     1681 cgcgggccga tgagctgctg acacagagcc tgaagcaggc gctgagcctg gtggatgtgc
     1741 gggtgatcga ccatttcgtc gtcgccggcg acacggtggt gtcgtttgcc gaacgcgggc
     1801 tggtgtaacg ccatgcgccg ccggccaacc gcgtcgcgtc cgatgctcaa aggcatgcc
//
LOCUS       DAOSIT010000499         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54170_length_1857_cov_9.672586, whole genome shotgun sequence.
ACCESSION   DAOSIT010000499 DAOSIT010000000
VERSION     DAOSIT010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54170_length_1857_cov_9.672586"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1857)
                     /locus_tag="PLL14_10395"
     CDS             complement(<1..>1857)
                     /locus_tag="PLL14_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOG04380.1"
                     /translation="IFQWRSLKTRVTIFTLAIFLVSLWSLAFYAGRMLRQDMQRLLGE
                     QQFSTVTFVAATLEDELKDRLHALEHSAGSIDSRLLERPAALQQFLEQRPILQVLFNA
                     GVVALTPAGRALAAVPVSSATPGVDYLTREVLAATLREGRSMISEPVIGGRLKQPAFA
                     MLAPIRDQHGQVIAVLLGLTHLGSPNFLDRITANRYGKSGGYLLIDPQRRLIVTATDR
                     SRRMEVLPAPGINPVIDRFIEGYEGSAVMVNPLGVEALVSDKRVALAGWIMAATLPTD
                     EAFAPIRDMQQHMLLATLMLTLLVAGLTWWMLRRELSPLLAAARALLTLPAEGEPLQP
                     LPVARDDEIGQLIGGFNRLLETLTQRERALKESQVFKNVILNSVPSEIAVLDRDGVIV
                     TVNEPWTRFATEHGTEPGKPASRTGIGVNYLSVCQAGLVSQANGGELNALEGIRAVLN
                     GKLASFTLEYPCNTPEQRYWFNLMVTPLGQDGDGAVVCHSNITARKLAEQTLAESERR
                     FAQFMDALPAAAFIKDQDGNTLYINRYLAEVIGASAWLGKSTRELFPPDVAERMIADD
                     RQALEAGFLDTEEPVLGADGTLRLYQTHKFRITRQAGPPLLGGIALDITERK"
BASE COUNT          358 a          579 c          586 g          334 t
ORIGIN      
        1 tgcttgcgtt cggtgatgtc cagcgcgatg ccaccgagca agggcggtcc ggcctgccgg
       61 gtgatacgga acttgtgggt ctggtagagc cgcaacgtgc catcggcgcc aagcaccggc
      121 tcctcggtgt ccaggaaacc ggcttccagc gcctgccgat catcggcgat catcctttcc
      181 gccacatcgg gagggaagag ttcgcgcgtg gattttccaa gccacgcgct ggcgccaatg
      241 acctccgcca ggtagcgatt gatatagagc gtgttgccgt cctggtcctt gatgaacgcg
      301 gctgccggca aggcatccat gaattgggca aaacgccttt cgctttcggc aagcgtctgt
      361 tcggccagtt tgcgcgcggt gatgttggaa tggcagacca cggcgccgtc accgtcctgg
      421 ccgaggggag tgaccatcag gttgaaccag tagcgttgct ccggagtatt gcagggatac
      481 tcgagagtga aactggccag tttgccgttg agcacggccc ggatgccttc aagggcgttc
      541 aattctcccc cgttcgcctg ggaaaccagg cctgcctggc aaaccgacag gtagttcacg
      601 ccaattccgg ttcgagacgc gggtttgccg ggctcggtgc cgtgctccgt ggcaaaacgc
      661 gtccagggtt cgttgaccgt cacgatcact ccatcgcgat cgagcactgc gatttccgaa
      721 ggtacggaat tcaggatgac gttcttgaaa acctggcttt ccttcaacgc cctttcccgt
      781 tgcgtgaggg tctccagcag gcggttgaag ccgccgatca gttggccgat ctcgtcatcg
      841 cgggcgacgg gcaaaggctg cagcggctcg ccttcagccg gcagggtcag cagcgccctg
      901 gcggcggcca gcagcggcga caactcgcgc cgcaacatcc accaggtcag accagcgacc
      961 agcagcgtca gcatgagcgt ggccagcagc atgtgctgct gcatgtcgcg gatcggggca
     1021 aaagcttcgt cagtcggcag agtggcagcc atgatccaac cggccaaggc aacccgcttg
     1081 tccgacacca gggcctccac gccaagcgga ttgaccatca ctgcggaacc ctcatagcct
     1141 tcgataaaac ggtcgatgac cggattgatg ccgggcgcgg ggagcacttc catgcggcgg
     1201 ctcctgtcag tcgcggtgac gatcaggcgg cgctgcggat cgatgagcag atagccgccg
     1261 ctcttgccgt agcggttagc cgtgatccgg tccagaaaat tggggctccc caggtgggtc
     1321 agccccagca ggacagcaat cacctggcca tgctggtcac gaatcggtgc cagcatggcg
     1381 aaagcgggtt gcttgagtcg cccgccgatg accggttcgc tgatcatcga ccggccttcc
     1441 ctgagcgtcg cggcgagcac ctccctggtc agataatcaa caccaggggt ggccgaggaa
     1501 acggggaccg cggcaagcgc cctgcccgcg ggcgtcagcg caacgacccc ggcgttgaac
     1561 aggacctgca ggatcggacg ctgttccaga aattgctgca aggcagcggg ccgttcgagc
     1621 aaccgggagt caatactgcc ggcgctgtgt tcaagcgcat gcagacggtc cttcaactcg
     1681 tcttccagcg tcgccgcgac aaatgtcacc gtcgagaact gctgctcgcc cagcagtcgt
     1741 tgcatatcct gccgcagcat ccggccggcg tagaacgcca gcgaccacag gctcaccaga
     1801 aagatggcca gggtgaagat cgtcaccctg gtcttgagcg accgccactg aaagata
//
LOCUS       DAOSIT010000500         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54370_length_1852_cov_6.638286, whole genome shotgun sequence.
ACCESSION   DAOSIT010000500 DAOSIT010000000
VERSION     DAOSIT010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54370_length_1852_cov_6.638286"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLL14_10400"
     CDS             <1..229
                     /locus_tag="PLL14_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04381.1"
                     /translation="VLDDLFKLGERRFEFQLSHACCCRASGFYVDRSLRVCSWHFKGS
                     NQTGENSLLVGFRSNGKLAAQDVRSTDDTLL"
     gene            365..724
                     /locus_tag="PLL14_10405"
     CDS             365..724
                     /locus_tag="PLL14_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOG04382.1"
                     /translation="MIVIEQQPKRVEVTVYAEFSLADYKEFENAVNFAIQFEGPVDLL
                     FDLREMADVTLDVAWEDIVFARAHANDFNRIAVLTHSQWVAWSAWLAQIFVRAETRIF
                     DDELDARAWLADEEGSA"
     gene            721..1569
                     /locus_tag="PLL14_10410"
     CDS             721..1569
                     /locus_tag="PLL14_10410"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOG04383.1"
                     /translation="MIPTTLVSSARLADRLEDPLWRVFDCRHLLSDPAAGRAAYARSH
                     LPGAFFLHLDEDLSGPTNGRNGRHPLPDPLLLAGKLGAAGVSAETQVVAYDDSGGMFA
                     ARLWWLLRWRGHARVAVLDGGIDQWIGEGRAVSTAVPTSTPAILEVDLRDGVVSSDDV
                     LAGLGAECFRVIDARSPDRFRGENETLDAVGGHIPGAINRFFRDNLDASGHFRPAIDL
                     RREFLALLAGIDPAQVVMSCGSGVTACHNLLAMEIAGLPGARLYAGSWSEWCSEPTRP
                     VARQIP"
     gene            complement(1574..>1852)
                     /locus_tag="PLL14_10415"
     CDS             complement(1574..>1852)
                     /locus_tag="PLL14_10415"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG04384.1"
                     /translation="LEGDCHAPMLVYESRSLGITARTEIAAVSDRYQQDWQATLEQLA
                     AKGKLRLPAKPMRLLLFGMLNWSRQWYRPNGGLSLDEIAEAAAEMVLA"
BASE COUNT          308 a          568 c          587 g          389 t
ORIGIN      
        1 tgttctggac gatctgttca agctcggcga gcgccgcttc gaatttcagc tcagccatgc
       61 ctgttgctgt cgtgccagtg gtttctacgt cgacaggagt cttcgagttt gttcgtggca
      121 ttttaaaggc tcgaatcaaa ctggcgaaaa tagcctatta gtgggcttcc ggtcaaatgg
      181 aaaactggcg gcgcaggatg ttcgatcaac tgacgatacc ctcctgtagc gccaccgcgc
      241 tgacgtgatg ccgcgtagcg ctcggccggg tgtttgctgc aaggccctgg gcgccatacg
      301 gatgcgccgc agacctgccg ggcgggactg gtgctaaact ggaacaacaa aaaaggagtc
      361 tgccatgatt gtcatcgaac aacagccgaa gcgcgtcgaa gtcacggtct atgccgagtt
      421 cagtcttgcc gattacaagg agttcgagaa cgccgtcaat ttcgcaatcc agttcgaggg
      481 gccggtcgac ctcctcttcg acctccgcga aatggccgac gtcacactgg acgtggcctg
      541 ggaagacatc gttttcgcac gcgcgcatgc caacgacttc aatcgcattg cggttctcac
      601 ccatagccag tgggtggcct ggagcgcctg gctggcgcag atcttcgtcc gtgccgagac
      661 gcgcatcttc gacgatgaac tcgacgcgcg tgcctggctg gctgacgagg agggctcggc
      721 gtgattccga caaccctggt cagcagcgca cggcttgccg atcgtctcga agatccgctc
      781 tggcgcgtct tcgactgtcg ccacctgctg agcgatcctg ccgccgggcg cgcggcctac
      841 gccagatctc acctgccggg ggctttcttt ctgcatctcg atgaggacct gtcggggccg
      901 accaacggtc gcaatgggcg tcatccgctg cccgatccgc tgctcctcgc cggcaagctt
      961 ggcgcggcag gcgtctcggc tgaaacccag gtagttgcct atgacgattc tggcggcatg
     1021 tttgctgctc gcctctggtg gctgttacgc tggcgggggc acgcgcgggt cgccgtgctc
     1081 gacggcggca tcgatcagtg gattggcgaa ggcagggccg tctcgaccgc tgtgccgacg
     1141 tccaccccgg caatcctgga ggttgatctg cgtgacgggg tggtgagcag cgacgacgtg
     1201 ctcgcaggtc ttggcgccga gtgcttccgt gtcattgacg cccgcagtcc cgaccgtttt
     1261 cgtggcgaga atgaaaccct cgacgccgtc ggggggcaca ttcccggcgc aatcaaccgc
     1321 tttttccgcg acaaccttga cgcgagtggc cattttcgcc ccgcgatcga cctgcgtcgc
     1381 gagttcctgg cgctgctcgc cgggattgat ccggcgcagg tggtcatgtc ctgtggttct
     1441 ggcgtgactg cctgtcacaa tctgctggcg atggagattg ccggtttgcc gggggcacgc
     1501 ctctatgccg gctcgtggag cgaatggtgc agcgaaccga cgcggccggt cgcgcgtcag
     1561 attccctgaa acctcaagcc agcaccatct cggctgccgc ctcggcgatc tcgtcgagcg
     1621 agagtccgcc gttgggtcgg taccactggc ggctccaatt gagcatgccg aaaagcagga
     1681 gccgcatcgg tttcgccgga aggcgcagtt ttcctttggc cgccaactgc tcaagtgtgg
     1741 cctgccagtc ttgctgatag cggtcgctga cggcagcgat ttcggttctt gccgtgatgc
     1801 ccagcgaacg cgactcatag accagcatcg gtgcgtggca atcgccttcg ag
//
LOCUS       DAOSIT010000501         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54587_length_1846_cov_11.018425, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000501 DAOSIT010000000
VERSION     DAOSIT010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54587_length_1846_cov_11.018425"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /gene="guaA"
                     /locus_tag="PLL14_10420"
     CDS             complement(<1..1505)
                     /gene="guaA"
                     /locus_tag="PLL14_10420"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOG04385.1"
                     /translation="MSHRKILILDFGSQVAQLIARRVREQQVYCELHPYDVTPQFIRN
                     FDPQGIILSGGPNSVYAVEDFKAPPVVFELGVPVLGICYGMQTMAAQLGGQVESSTRR
                     EFGYAEMRAHGHCTLFKGIEDRRNAEGHGLLDVWMSHGDKVTALPPGFKVIGSNASTA
                     IAAMADEARRFYAVQFHPEVTHTLKGREIIGRFVRDICGCGHDWNMPDYVTEAIEKIR
                     SQVGNEEVILGLSGGVDSSVAAALIHRAIGEQLTCVFVDNGLLRLNEAEQVMQTFARN
                     LGVKVVHVDAAAQFMGQLQGVSDPEEKRKIIGREFVEVFQVEAARLSNAKWLAQGTIY
                     PDVIESAGAKTRKAHTIKSHHNVGGLPEQLNLWLLEPLRELFKDEVRELGIALGLPHD
                     MVYRHPFPGPGLGVRILGEVKPEFAELLRRADAIFIDELRAAGWYEKTSQAFAVFLPV
                     KSVGVMGDGRTYEYVVALRAVETQDFMTAHWAELPHSLLGKVSNRIINEVRG"
     gene            complement(1565..>1846)
                     /locus_tag="PLL14_10425"
     CDS             complement(1565..>1846)
                     /locus_tag="PLL14_10425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG04386.1"
                     /translation="AGAADRYFQEETANVDKLVPEGIEGRVPYKGSVLAVVHQLMGGL
                     RSSMGYLGCRTIAEMHAKAAFVEITSAGVRESHVHDVQITKEAPNYHVD"
BASE COUNT          313 a          582 c          592 g          359 t
ORIGIN      
        1 ccacgcacct cgttgatgat ccggttggaa acctttccga gcaggctgtg cggcagttcc
       61 gcccagtgcg cggtcatgaa gtcctgcgtt tccacggcgc gcagggcgac gacgtattcg
      121 taggtccgac cgtcgcccat gacccccacc gatttcacgg gcaggaatac ggcaaaggcc
      181 tggctggttt tctcgtacca tcctgcggca cgcaactcgt cgatgaagat ggcgtcggcg
      241 cggcgcagga gttcggcaaa ctcgggcttc acttcgccga gaatgcggac gccgaggccg
      301 gggccgggga aggggtggcg gtataccatg tcgtgcggca ggccaagggc gatgccgagt
      361 tcgcgtactt cgtccttgaa cagttcgcgc aggggctcga gcagccagag gttgagctgc
      421 tcgggcaggc cgccgacgtt gtgatggctc ttgatggtgt gcgccttgcg ggtcttcgcg
      481 cctgccgatt cgatcacgtc ggggtagatc gtgccttgcg ccagccattt ggcgttgctg
      541 agcctggcgg cctcgacctg gaagacctcg acgaactcgc gaccgatgat cttgcgcttc
      601 tcttccgggt cggagactcc ctgcaactgt cccatgaact gcgcggcagc gtcgacgtgt
      661 acgaccttga cgccaaggtt gcgggcaaaa gtctgcatca cctgttccgc ttcgttgagt
      721 cgcagcagtc cgttatcgac gaacacgcag gtgagctgct cgccgatcgc gcggtggatc
      781 agcgccgccg cgaccgagga atcgacgccg cccgaaaggc ccaggatcac ctcctcgttg
      841 cccacctgcg aacgaatctt ctcgatcgct tcggtgacgt agtcgggcat gttccagtca
      901 tgcccgcagc cgcagatgtc gcggacgaaa cgtccgatga tttccctgcc cttgagcgtg
      961 tgggtgacct ccggatggaa ctgcacggcg tagaagcggc gtgcctcgtc ggccatcgcc
     1021 gcgatcgctg tcgatgcatt gctgccgatc accttgaagc ccggtggcaa cgcggtgacc
     1081 ttgtcgccat ggctcatcca gacgtcgagc aggccatgcc cctcggcatt gcgccggtcc
     1141 tcgattccct tgaacagtgt gcagtggccg tgcgcacgca tttcggcgta gccgaattcc
     1201 ctgcgcgtcg agctttcgac ctggcctccc agctgtgcgg ccatcgtctg catgccgtag
     1261 cagatgccca ggacggggac acccagttcg aacaccaccg gcggcgcctt gaagtcctcg
     1321 accgcataaa ccgaattcgg tccgcccgac agaatgatcc cctgcggatc gaagttgcgg
     1381 atgaactgtg gcgtgacgtc gtagggatgc agctcgcaat acacctgctg ttcgcggacg
     1441 cggcgggcga tcagttgcgc gacctgcgag ccgaagtcga ggatgagaat tttccggtgg
     1501 gacatgggcc aggcctcaaa tgcagcgggc ggcctgcagc cgcccgctgg tcgtcgatcg
     1561 atactcaatc gacgtggtaa ttgggagctt ccttggtgat ctgaacgtca tggacgtgcg
     1621 actcgcggac gcctgccgag gtgatctcga cgaaggccgc cttggcgtgc atttccgcga
     1681 tggtccggca gccgagatag cccatcgatg aacgcaggcc gcccatcagt tgatgaacga
     1741 cggccagcac cgaccccttg tagggaaccc ggccctcgat gccttcgggc accagcttgt
     1801 cgacgttggc cgtttcctcc tggaagtagc ggtcggcggc gcccgc
//
LOCUS       DAOSIT010000502         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54622_length_1845_cov_12.974860, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000502 DAOSIT010000000
VERSION     DAOSIT010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54622_length_1845_cov_12.974860"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLL14_10430"
     CDS             <1..180
                     /locus_tag="PLL14_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04387.1"
                     /translation="DLIVVAPARDELDDPRIAAAAARLGPIQTLTAHKNGIAVGTYHA
                     RVVYGYRPPTPAPRQ"
     gene            177..1412
                     /locus_tag="PLL14_10435"
     CDS             177..1412
                     /locus_tag="PLL14_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOG04388.1"
                     /translation="MNVNLQRAIDRYLGVPLCALASLLERVLPRRTPPSLPRRIVVIL
                     LSEMGSLVLAQPMFALLRDRYPGASLHVLLFGKNREVLDLMASVPPENVITIDDRSLG
                     GLLRTLFAAIRTLRRLRPDAVLDCELFARISSLLAWLSGAPLRVGFHRHTQEGLYRGS
                     FINRRVLYNPYRHLSLQFLTLARALVSKTHPLDKEQQDPGDFPLTAPLPGFTAGEVDA
                     FQARIVDDYPRFSGRRLALLYPSGGILPIRAWPPENWRMVASGLLAEGYVVAIIGLPA
                     DKPLGRALSEAVAHPDCVDLTGYTRSVRELVALLGRAALLVTNDGGPAQFSSLTGVPT
                     VALFGPETPLLYGPLGDHASCLHLALPCSPCLSAYNHRNSPCDGDNQCLRRIAPERVL
                     TEARAVVARIRPGSIKDTP"
     gene            1409..>1845
                     /locus_tag="PLL14_10440"
     CDS             1409..>1845
                     /locus_tag="PLL14_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG04389.1"
                     /translation="MTRFERLHHLPLVGRLLRHRFIKFGVVGASGTVVNVVVLYLAQE
                     ILFRAIEPPPLRLNVSLAVAIFLATINNFLLNRAWTWLDRHEHVQTPLLLQFGQYTIA
                     CWLGIALQFALTHAFASHMHYLAANVLSIVIASVANFVVNDHWT"
BASE COUNT          280 a          667 c          539 g          359 t
ORIGIN      
        1 gacctgatcg tcgtcgcgcc ggcacgcgac gaactcgacg atccgcggat agccgccgcc
       61 gccgcccgcc tgggtccgat ccagaccctc acggcgcaca agaacggcat cgcggtcggc
      121 acctaccatg cgcgggtcgt ttacgggtat cggcccccca ctccagcgcc tcggcaatga
      181 acgtcaatct ccagcgcgcc atcgatcgct atctcggcgt gccgctctgc gcacttgcct
      241 cgttgctcga gcgcgtgctg ccacggcgca ccccgccatc tttgccacgc cggatcgtcg
      301 tgatcctgct ttccgagatg ggcagcctgg tgctcgcgca gcccatgttc gcgctattgc
      361 gcgaccgcta ccccggggct tcgctgcatg ttctgctgtt cggaaagaac cgcgaagtgc
      421 tcgacctgat ggccagcgtc cctccggaaa acgtcatcac catcgacgac cgctccctcg
      481 gcggtctcct gcggacgctc ttcgccgcaa tccgcacgct gcgtcggctg cgcccggatg
      541 cggtcctcga ctgtgaacta ttcgcgcgca tcagcagcct gctggcctgg ctgtcgggag
      601 ctccattgcg cgtgggcttt caccgccaca cgcaggaagg actgtaccgc ggttcgttca
      661 tcaaccggcg agtgctctac aacccttatc gccacctttc actgcagttc ctgaccctgg
      721 cacgcgcact cgtttcgaaa acccaccccc tcgacaagga gcagcaggac ccgggcgatt
      781 tccccctgac cgccccgtta ccgggcttca ccgcgggcga ggtggatgcc ttccaggcgc
      841 ggattgtcga cgactatcca cgcttctccg ggcgacgcct ggcgctgctc tatccgagcg
      901 gcggcatcct gccgatccgc gcatggccgc cagagaactg gcgcatggtc gcatccggcc
      961 tgctcgccga gggatatgtg gtggcgatca ttggcttgcc tgccgacaag ccactcgggc
     1021 gcgcgcttag cgaggcggtc gcgcacccgg attgtgtcga cctcacgggc tacacccgct
     1081 cggtgcggga actcgttgca ctgctcggaa gggctgcgct gctggtcacc aacgacggtg
     1141 ggccggcgca gttttcgtcg ctcaccggcg tgccgaccgt agccttgttt ggcccggaga
     1201 cgccgctgct ctacggtccg ctcggtgacc acgccagctg cctgcatctg gcgctgccct
     1261 gttcaccgtg tctctccgcg tacaaccacc gcaactcgcc ttgcgacggc gacaaccagt
     1321 gcctgcggcg gatcgctccc gagcgggtac tgacggaagc tcgcgccgtc gtcgcgcgta
     1381 tccgcccggg gtcgatcaag gatacaccat gacgcggttt gagcggctcc accatctacc
     1441 gctcgttggg cgcttgttgc ggcatcgctt catcaagttc ggcgtcgtcg gcgcctccgg
     1501 aacggtggtc aacgtcgtgg tgctctacct ggcccaggaa attcttttcc gggccatcga
     1561 accaccaccg ctgcgcctga acgtatcact cgcggtcgcc atcttccttg ccaccatcaa
     1621 caatttcctg ctcaatcgcg cgtggacctg gcttgaccgg cacgaacatg tgcagacgcc
     1681 gcttctcctg cagttcggcc agtacaccat cgcctgctgg ctcgggatcg cgctgcagtt
     1741 cgccctgaca cacgcgttcg ccagccacat gcattatctg gcggcaaatg tgctttcgat
     1801 cgtgatcgcc agcgtggcga acttcgtggt caacgaccac tggac
//
LOCUS       DAOSIT010000503         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54628_length_1845_cov_6.310615, whole genome shotgun sequence.
ACCESSION   DAOSIT010000503 DAOSIT010000000
VERSION     DAOSIT010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54628_length_1845_cov_6.310615"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PLL14_10445"
     CDS             complement(<1..1824)
                     /locus_tag="PLL14_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG04390.1"
                     /translation="MSAPPTPAPSRLLMSGNEAVARAVWEAGVRVAAAYPGTPATEML
                     ECVAGYREIYAEWSVNEKVSLEVAFGAAMAGSRSFCAMKHVGMNVASDALMTMTLTGV
                     AGGLVIAIADDVGLSSSQNEQDSRFWGRFAHVPVLEPADSQEAHDFTLAAFALSERFE
                     TPVILRLTTRICHVKGLVSVGERSERPVAGFTKDVGRWVMVPSAAGRRLPLMFDRERR
                     LRAEAEITELNIFEPGSDRRVGFIASGPAYMHVRESFPNAPVFKLGFSFPLPFDSLRQ
                     FASQCDRLVVVEEVEPLIETELKAQGMNVSGKDILPPSGELSPQVLKPAVAGLLGEAL
                     PELAGRAPMPVFPRPPTMCVACPHLGVYYTLSQLRNVTISGDIGCYTLGFGQPWNALD
                     ACISMGASMGMALGLDKGRSDAEKDKKIVAVIGDSTFLHMGMQGLLDIVYNKGNVTVL
                     LLDNRAVGMTGGQDNPGNGRDIHGDPAPRVDFAKLVEALGVKQERIHVVDPYQLPVLF
                     KTIRDEVKVPEVSVIITDQPCVLIKDYHKLKAYEVIDDKCTGCGNCIDVGCPAIHVTR
                     RGKEVKASGREVDLAFVRIETSVCTGCGLCVQPCAPNAIVHH"
BASE COUNT          332 a          604 c          600 g          309 t
ORIGIN      
        1 gtgatggacg atggcattcg gcgcgcaggg ctgcacgcac aggccacagc cggtgcacac
       61 ggaagtctcg atgcgcacaa aggcaagatc gacctcgcgc ccactggcct tgacctcctt
      121 ccctcttcgg gtgacgtgga tggccgggca gccgacatcg atgcaattgc cgcaaccggt
      181 gcacttgtcg tcgatgacct cgtaggcctt gagcttgtga tagtccttga tcaggacgca
      241 gggctggtcg gtgatgatga ccgagacttc cggcaccttg acttcgtcgc ggatggtctt
      301 gaacagcacc ggcaactggt agggatcgac gacgtggatg cgctcctgtt tgaccccgag
      361 cgcctcgacc agcttggcga agtctacgcg cggcgccggg tcaccatgga tgtcgcggcc
      421 gttgccgggg ttgtcctggc cgccggtcat gccgaccgcg cggttgtcga gcagtagcac
      481 ggtgacgtta cccttgttgt agacgatgtc gagcagcccc tgcatgccca tgtgcaggaa
      541 ggtcgaatcg ccgatcacgg cgacgatctt cttgtccttc tcggcgtcgc tgcgcccctt
      601 gtcgaggccc aacgccatgc ccatcgaagc gcccatcgag atgcatgcgt cgagggcgtt
      661 ccatggttga ccgaagccca gcgtgtagca gccgatgtca cccgagatgg tgacgttgcg
      721 caactgcgac agcgtgtaat acacccccag atgcggacag gcgacgcaca tcgtcggcgg
      781 gcgcggaaag accggcatcg gcgcgcgtcc ggcgagttcg ggtagcgcct cgccaagcag
      841 accggcgacc gccggtttga gtacctgcgg cgacaactcg ccggatggcg gcaggatgtc
      901 cttgccgctg acgttcatgc cctgcgcttt cagttcggtc tcgatcagcg gctcgacctc
      961 ttcgacgacg accaggcgat cgcactggct ggcgaactgg cgcaggctgt cgaaaggcag
     1021 cggaaaggaa aaaccgagct tgaacaccgg cgcgttcggg aagctctcgc gaacgtgcat
     1081 gtacgccggc ccggaggcga tgaagccgac ccgacgatcg ctgcccggtt cgaagatatt
     1141 gagttcggta atctcggctt cggcgcgcaa ccggcgttcg cggtcgaaca tcagcggcag
     1201 acgtcgaccg gcggcgctcg gcaccatcac ccagcgaccg acatccttgg tgaaaccggc
     1261 aaccggccgc tcgctgcgct cgccaacgct gaccaggccc ttgacgtgac agatccgcgt
     1321 cgtcaggcgc aggatcaccg gcgtctcgaa gcgctccgaa agggcgaaag cggcgagcgt
     1381 gaagtcgtgc gcttcctgcg aatcggccgg ttcgagcacg ggcacatgcg cgaaacgacc
     1441 ccaaaagcgc gaatcctgct cgttctgcga cgacgacagg ccgacgtcat cggcgatggc
     1501 gatcaccaga ccgccggcga cgccggtcag ggtcatggtc atcagggcgt cggaagcgac
     1561 gttcatgccc acgtgcttca tcgcgcagaa gctgcgcgac ccggccatcg cggcgccgaa
     1621 ggcgacttcg agcgaaacct tctcattgac cgaccactcg gcgtagattt cgcgatagcc
     1681 cgccacgcac tccagcatct cggtcgccgg ggtgccggga taggccgccg ccacccgtac
     1741 gccggcctcc catacggcac gcgccaccgc ctcgttgccg gacatcagaa gccggctggg
     1801 cgctggcgtg ggcggggcgg acatcactgc tgctgccgtc atggc
//
LOCUS       DAOSIT010000504         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54949_length_1837_cov_9.314815, whole genome shotgun sequence.
ACCESSION   DAOSIT010000504 DAOSIT010000000
VERSION     DAOSIT010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54949_length_1837_cov_9.314815"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1837
                     /locus_tag="PLL14_10450"
     CDS             <1..>1837
                     /locus_tag="PLL14_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOG04391.1"
                     /translation="VTGAADVRIFGERKYAMRVWLDPDKLAAYKLTTQDAEDAIRRSN
                     LEVPAGRIESTQREFSVTSQTDLTRPSQFSDIIIKNANGFPVRIRDVGRVQEAAADER
                     SGSRLNGKVAITVGVIRQATANPLELSRGVRAMIPKLKADLPPGISIDVANDNSVFID
                     KSIQSVFQTILEAIVLVALVIFVFLRTVRASIIPIITIPVCLIGTFALMALAGFTINT
                     LTLLALVLAIGLVVDDAIVVLENIYRHIEEGMDPFSASIKGVREVGFAVVAMTLTLVA
                     VYAPLAFTPGRTGRLFIEFALALAGAVAVSGFIALTLSPMMCSVLLRHNPNPSWFDSH
                     MERWLNALTAGYQRVLTWLVTTRYDRSRSQVGAGHSLRRMMMDARWIVIAVMVLSGIG
                     IAIVLPGMKRELSPLEDRGQVLAVVTAPDGATLDYTNRYVGAIERMGQPYKEFDRIFV
                     SSGNPTVSQANVFFRAIDWDQRSRSVLDVARELQPKMAGLAGVTAFPITPPSLGQGFR
                     ERPVNFVLITTDSYQNLAAVTRQFMDEIGKNPGIVSADIDLRLNKPELKIDVNREKAA
                     DLGVSVDVVARAIETMLGGRQVTRYKREGDQFDVIVQTQASGRDTP"
BASE COUNT          311 a          594 c          599 g          333 t
ORIGIN      
        1 gtgaccggcg cagccgatgt gcgcatcttc ggcgagcgca agtacgccat gcgcgtctgg
       61 ctcgaccccg acaagctggc cgcctacaag ctgaccaccc aggacgccga ggatgccatc
      121 cggcgcagca acctggaggt gccggccgga cgcatcgagt ccacccagcg tgagttcagc
      181 gtgacctcac agaccgacct gacgcggccg tcccagtttt ccgacatcat catcaagaat
      241 gccaacggct tcccggtccg catccgcgac gtgggacgcg tgcaggaagc agccgctgac
      301 gagcgcagcg gctcacgcct caacggcaag gtggccatca cggtcggggt gattcgtcag
      361 gccacggcca acccattgga gctgagccgc ggcgtgcgcg ccatgatccc caagctcaag
      421 gccgacctgc cgcccggcat ttcgatcgat gtcgccaacg acaactcggt gttcatcgac
      481 aagtccatcc agagcgtgtt ccagaccatt ctggaggcga tcgtgctggt ggccctggtg
      541 atctttgttt tcctgcgcac tgttcgcgcc tccatcatcc cgatcatcac catcccggtg
      601 tgtctgatcg gcacctttgc gctcatggcg ctggccggtt tcaccatcaa cacgctgacc
      661 ctgttggcac tggtgctggc gatcggcctg gtggtcgacg atgcgatcgt ggtgctggag
      721 aacatctacc ggcacatcga ggaaggcatg gatccgttct cggcctcgat caagggggtg
      781 cgggaggttg gcttcgcagt cgtggccatg acgctgacgc tggtggcggt gtatgcgccg
      841 ctggcgttca cgccggggcg caccggtcgc ctgttcatcg agtttgccct cgcgctggcc
      901 ggggcggtgg ccgtgtcggg cttcatcgcc ctgacgctgt cgccgatgat gtgttcggtg
      961 ctgctgcgcc acaacccgaa tccgagctgg ttcgacagcc acatggagcg ctggctcaat
     1021 gccctgaccg cgggttacca gcgggtgctg acctggctgg tgaccacacg ctacgaccgc
     1081 agccggtctc aggtgggtgc aggtcattcg ttgcgcagga tgatgatgga tgcacgctgg
     1141 atcgtgatcg ccgtgatggt gctctcgggc atcggtatcg cgatcgtgct gcccggcatg
     1201 aaacgcgaac tgtcgccgct ggaggaccgg gggcaggtgc tggcggtggt cacggcaccg
     1261 gatggcgcca cgctggacta caccaatcgc tatgtcggtg ccatcgaacg catgggccag
     1321 ccctacaagg agtttgaccg gatcttcgtc tcctccggca accccacggt gtcccaggcc
     1381 aacgtgttct tccgcgccat cgactgggac cagcgcagcc gctccgtgct cgacgtcgcg
     1441 cgcgagctgc aacccaagat ggccggactg gccggggtga cggctttccc gatcacgccg
     1501 ccttcgctgg ggcaggggtt tcgggagcgt cccgtcaatt tcgtgctgat caccaccgac
     1561 agttaccaga atctggccgc ggtgacgcgg cagttcatgg acgagattgg caagaaccct
     1621 ggcatcgtca gcgccgatat cgacctgcgc ctgaacaagc ccgagctcaa gatcgatgtc
     1681 aaccgggaaa aagcggcgga cctgggcgtc agcgtcgacg tcgtggcccg ggcgatcgag
     1741 accatgctgg gcggccggca ggtgacccgc tacaaacgcg agggcgacca gttcgacgtg
     1801 atcgtgcaga cgcaggcctc gggccgcgat acgcccg
//
LOCUS       DAOSIT010000505         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_54977_length_1836_cov_7.638405, whole genome shotgun sequence.
ACCESSION   DAOSIT010000505 DAOSIT010000000
VERSION     DAOSIT010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54977_length_1836_cov_7.638405"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLL14_10455"
     CDS             <1..387
                     /locus_tag="PLL14_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG04392.1"
                     /translation="DVTGFGLLGHLVEICKASQVSAEIDFAALPVLPNALDYAAKGCV
                     TGASERNWASYGEHVTLPKKQFGDLERALLTDPQTSGGLLVACAPEVVTEVLAIFLQE
                     GFDRAAVIGGISEGKPQVSVLKGKAE"
     gene            complement(475..1719)
                     /locus_tag="PLL14_10460"
     CDS             complement(475..1719)
                     /locus_tag="PLL14_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOG04393.1"
                     /translation="MSLDDSLIRPFAGLRPRGADAAAVAAPPYDVLSSAEARHLAIGK
                     PLSFLHVSKAEIDLPPEIDHHAPEVYARSAENFQRLIVGGVLRRDSSPCYYVYRLCMG
                     EHVQTGLVAVASIAAYDSNRIRKHEFTRPDKEDDRVRQIEALNAQTGPVLLAHPDSPQ
                     AEHLIASVTDAMPVADVLADDGIRHTLWVIDDAQRIAAISEVFGAMRTLYIADGHHRS
                     AAASRVAAARRGGSHATAGAEYFLSVIFPASQMLIMDYNRVLRDLNGLSEDAFLAAVG
                     ERYTVTASDARVRPQRSGVCGMYLQGRWYRLEIQPGLVPASDPVRRLDVSVLSEQLLG
                     PVLGITDLRRDTRIDFVGGMRGLAELERRVDSGEMALAFAMYPTQMADLMAVADAGQV
                     MPPKSTWFEPKLADGLVSHLLY"
BASE COUNT          372 a          583 c          570 g          311 t
ORIGIN      
        1 gacgtcacgg gctttggcct gctcggtcac ctggtggaaa tctgcaaggc ctcgcaggtt
       61 tcagccgaga ttgatttcgc ggccttgccg gtcttgccga acgctctcga ttatgcagcc
      121 aagggctgcg tcaccggtgc ttccgaacgt aactgggcga gttacggcga gcacgtgaca
      181 ctcccaaaga agcagttcgg tgacctcgaa cgcgcgctgc tgaccgaccc gcaaaccagc
      241 ggtggactgc tggtcgcgtg tgcaccggaa gtggttaccg aggttctggc aattttcctg
      301 caggagggct tcgatcgcgc cgcagtgatt ggcgggattt ccgaaggcaa accacaggtc
      361 tccgtgctca agggcaaggc ggaatgacgt cctgcaaaag gccctgatcg gcgagcccgt
      421 gccaccgccg tcaagatcgg ctggcgcgct cccagaccag ttctaccagc acactcaata
      481 gagaaggtgc gaaaccaggc cgtcagcaag cttcggttca aaccacgtgg acttcggtgg
      541 catgacctga cccgcgtcag ccactgccat cagatcggcc atctgcgtcg gatacatggc
      601 aaaagccagg gccatttctc cgctgtccac ccgccgctcg agttccgcaa gcccgcgcat
      661 gccaccgacg aaatcgatgc gcgtgtcccg acgcagatcg gtaatgccca gtaccggccc
      721 gagaagctgc tcggatagta ccgagacatc gagccggcgt accgggtccg aagcaggcac
      781 cagaccgggc tgaatctcca gccgatacca gcgcccctgg agatacatgc cacatacgcc
      841 tgaacgttgc ggcctgaccc gtgcatcgct cgcggtgacg gtatagcgct cgccaaccgc
      901 cgccagaaac gcgtcctcgg acaggccgtt cagatcccgc aacacgcggt tatagtccat
      961 gatcagcatc tgcgacgccg ggaagatgac cgacaggaaa tattcggcac cggccgtcgc
     1021 gtggctgccg ccacgtcggg cggcagccac gcgcgaggcg gcagccgaac ggtgatgacc
     1081 gtcggcaatg tacagcgtgc gcatggcgcc aaacacctca ctgatcgcgg cgatgcgttg
     1141 cgcgtcatcg atcacccaca gcgtatgcct gatgccgtca tcggccagca cgtcggcgac
     1201 cggcatggcg tcggtgaccg acgcgatcag atgctctgcc tgcgggctgt ccggatgggc
     1261 gagcaacacc gggccggtct gtgcattcag cgcctcgatc tggcgcaccc gatcgtcttc
     1321 cttgtctggc cgggtgaatt cgtgcttgcg aatgcgattg ctgtcgtagg cggcaatcga
     1381 ggccaccgcc accaacccgg tctggacgtg ctcacccatg cacagccggt aaacgtaata
     1441 gcaggggctg gagtcacggc gaagcacacc gccaacgatc aggcgctgaa aattctcggc
     1501 cgacctggca tacacctcgg gggcatggtg atcgatttcg ggaggcaggt cgatctcggc
     1561 cttcgagaca tgcaggaatg acaagggctt gccgatcgcc agatgccgtg cctcggcgct
     1621 agaaaggaca tcatagggcg gtgccgccac tgcggcggca tcagcgccac gtggtcggag
     1681 accggcaaaa gggcgaatca acgaatcatc aagtgacaag gaactctcct gcaagggctg
     1741 gtttcccgga gcacaggccg tttgcggtgc cgcgacctga gcggatgtca gatgctgcgg
     1801 tacagcgcct catagcgcac ggccgaatcg gcccat
//
LOCUS       DAOSIT010000506         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55264_length_1829_cov_13.383315, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000506 DAOSIT010000000
VERSION     DAOSIT010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55264_length_1829_cov_13.383315"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="PLL14_10465"
     CDS             complement(<1..1565)
                     /locus_tag="PLL14_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOG04394.1"
                     /translation="MRFLDLLAKPFRRAPRETAADRAAWIDGAVRHLAAQSHQARLAC
                     LRTASRSFEAGETPSWVSSWATTAAGINEDLHNQLPAMRARSRNLGRNNEWAKRYRIQ
                     LVDNVLGSAGIRLQMRLRQAARGRQQAAGTAPLDSEANALLEAAWARFGKRGNCEVSG
                     RLCWKEIETLMAWSLATDGEILYRFRPGAGPFRIQLQLLDPTLLDVTVRREYQGRRVR
                     MGVEIDDDGKPVAYWLRAAKAGDLGIDSSTVGAHVRIPAEQIRHRFLVEEVDQIRGVP
                     QLAIGARRLHMLHDFEDAAAVACSNSAKRLGFFVSPSGDAPPGFADQIVSSVLDAAHA
                     AGKVLTPDEIQQITASAEKYTTTVPGTFDTVPSGYDFRQYDSPWPNIDSTEYVKSQVR
                     GWSAAQGASYVSIGNDLADVNYSSARVGILDEREHYKELQSRLISWLHEDVFETVLPY
                     LAAATPGLQVSRLPDYLAAATWQARRWQGIDPVKESQADETNLQNGLTSRSRIIMARG
                     EDPDEIAAERAADV"
     gene            complement(1581..>1829)
                     /locus_tag="PLL14_10470"
     CDS             complement(1581..>1829)
                     /locus_tag="PLL14_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04395.1"
                     /translation="IAANLATATTFDGRSANVKALDDLNAAMASYCTAGHGPVAEYQI
                     GDRRMKFRSTTEIAELIAYYERQVAIERGGRRAVYYRG"
BASE COUNT          264 a          539 c          691 g          335 t
ORIGIN      
        1 acgtctgcgg cgcgctcggc ggcgatctcg tcggggtctt cgccgcgcgc catgatgatt
       61 cggctgcgcg atgtcaggcc gttctgaagg ttggtctcgt cggcttgcga ttccttgact
      121 gggtcgatac cctgccaacg gcgcgcctgc caggtggcgg cggcgaggta gtcggggagt
      181 ctcgagactt gcagcccggg agtcgccgcg gcgaggtagg ggagaacggt ctcaaaaacg
      241 tcttcatgaa gccaggaaat caggcgggac tgcaattcct tgtagtgctc gcgctcgtcg
      301 aggatgccga cgcgcgccga gctgtaattg acgtccgcga ggtcgttgcc gatcgagaca
      361 tacgaggcgc cctgagcggc tgaccagccg cgcacctggc ttttgacgta ctcggttgag
      421 tcgatgtttg gccagggcga gtcgtactgc cggaaatcgt agccgctcgg aacggtgtcg
      481 aatgtgccgg ggacggtggt ggtgtatttc tcggcgctgg cggtgatttg ctggatttcg
      541 tccggggtga gcaccttgcc ggcggcgtgc gcggcatcga ggacggatga cacgatctga
      601 tcggcgaatc cgggcggcgc gtcgccgctt gggctgacga aaaagccgag gcgcttggcg
      661 ctgttggagc aggcgacggc agcggcgtcc tcgaagtcgt gcagcatgtg caggcggcgc
      721 gcgccgatgg cgagctgcgg cacgcctcga atctggtcga cttcctccac cagaaagcgg
      781 tggcggattt gctcggcggg gatgcgcaca tgcgcgccga cagtgctgga gtcaatgccc
      841 aggtcgccgg ctttcgcggc gcgcagccag taggccaccg gcttgccgtc gtcgtcgatc
      901 tcgacgccca tgcgcacgcg gcggccctgg tactcgcggc gcacggtgac gtcgagcagc
      961 gtggggtcga ggagttggag ctgaatcctg aacggcccgg cgccggggcg gaatcggtag
     1021 aggatttcgc cgtctgtcgc gagagaccac gccatcaggg tctcgatttc tttccagcag
     1081 aggcggccgg agacttcgca attgccgcgc ttgccgaacc tggcccacgc ggcttcgagg
     1141 agcgcattgg cctcgctgtc gaggggagcg gtgccggcgg cttgctgccg tccgcgcgcg
     1201 gcctggcgca ggcgcatctg caggcggatg ccggcggagc cgagcacgtt gtcgacgagt
     1261 tggatccggt agcgcttggc ccactcattg tttcgcccca ggttgcgcga gcgggcgcgc
     1321 atcgccggca attggttgtg caaatcctcg ttgatgccgg cggcggtcgt cgcccaggac
     1381 gagacccatg acggcgtttc gccagcctcg aagctgcgcg aggcagtgcg cagacaggcc
     1441 agtctcgcct ggtgagactg cgcggcgagg tggcgcacgg cgccgtcgat ccacgcggcg
     1501 cgatcggcgg cggtttcgcg cggcgcgcgg cggaagggct tggcgaggag gtcgaggaag
     1561 cgcatgtcgt gggctcctgg tcagccgcgg taatagacgg ctcgccgtcc gcctcgctcg
     1621 atcgcgactt ggcgctcgta gtaggcaatg agctcggcaa tttcggtggt gctgcggaat
     1681 ttcatgcggc ggtcgccgat ctggtactcg gcgacagggc catgtccggc ggtgcagtag
     1741 ctggccatgg cggcattgag gtcgtcgagc gccttgacgt tggcgctgcg gccgtcaaag
     1801 gtggtcgcgg tggccaggtt ggccgcgat
//
LOCUS       DAOSIT010000507         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55266_length_1829_cov_9.018602, whole genome shotgun sequence.
ACCESSION   DAOSIT010000507 DAOSIT010000000
VERSION     DAOSIT010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55266_length_1829_cov_9.018602"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLL14_10475"
     CDS             <1..1322
                     /locus_tag="PLL14_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG04396.1"
                     /translation="WRDIREPAFLWQVLALGMSLLLAWGLSRYGRQQEFVRSTAEHGA
                     LRTFSTGSIKRVAFPLLSLLLIVIVRKLFKYWHLGPVSLFDLAVPLLLSMALVRAAIY
                     VLRHALTAGSWLATSERFVATSIWLCLALYLTGLADPLIEMLEQVSFHVGKQKLDLWT
                     LLNGLVTVVATVLLALWLSGVIERRLLASDALDSNVRVVLGRLVKAVLSVVALMLSLS
                     IVGIDITALSVFSGALAVGLGLGLQKIASNYVSGFIILLDRSIRIGNLVALDATTSGI
                     VTQITARYTVLRTLAGTEVIIPNEYLVSNIVRNESFTDSRLRLQVSVQVAYGTDLEQA
                     MRLMVEAARAQPRVLDDPAPQALLTAFADSGINLDLGFWIADPQEGTGGVRSAINLSI
                     WRSFCEQGIEIPFPQREVRLIGGLPPVATAPAEPAAPAVVPGLQPVA"
     gene            1555..>1829
                     /locus_tag="PLL14_10480"
     CDS             1555..>1829
                     /locus_tag="PLL14_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04397.1"
                     /translation="MQTGVGSGRRLLAGEITGRRCKNLQGLRAAPGAVIDQAEELQRY
                     AVVRDRLQDAARDPLRLRQIAALIERSQKVEQGIDHRINRARPARRSS"
BASE COUNT          287 a          592 c          605 g          345 t
ORIGIN      
        1 tgtggcggga cattcgggag ccggcgttcc tctggcaggt gctggccctg ggcatgagcc
       61 tgttgctcgc ctggggtctg tcgcgctacg gtcggcagca ggaattcgtc cgctcgacgg
      121 ccgaacatgg cgcgctgcgg acattcagca caggcagcat caagcgcgtg gccttcccgc
      181 tgttgtcgct gctgttgatt gtgatcgtgc gcaagctctt caaatactgg cacctcggtc
      241 cggtcagtct gttcgacctg gcggtgcccc tgctgctgtc gatggcgctg gtgcgagcgg
      301 cgatctacgt actgcgtcac gccttgactg cggggagctg gctggcgact tccgaacgct
      361 tcgtggccac cagcatctgg ctctgcctgg cgctgtatct gaccgggctg gcggacccac
      421 tgatcgagat gctcgaacag gtgagctttc atgtcggcaa acagaagctc gacctgtgga
      481 cgctcctgaa tggtctggtg accgtcgtcg ccacggtgtt gctcgccctc tggctgtccg
      541 gcgtcatcga gagacgcctg ctggcaagcg acgcgctgga ctccaatgtg cgggtggtgc
      601 tgggccgcct ggtcaaggcc gtgctgtcgg tggttgccct gatgctgagc ctgtcgattg
      661 tcggcatcga catcaccgcc ctgtcggtgt tcagcggtgc gctcgccgtc ggcctcggcc
      721 ttggactgca aaagatcgcc agcaattacg tcagcggctt catcattctg ctcgaccgct
      781 cgattcgcat cggcaatctg gtggcgctgg acgccacgac ctccggcatc gtgacccaga
      841 tcaccgcccg ctatacggtc ctgcgcaccc tggccggtac cgaggtgatc attcccaacg
      901 agtatctggt gtcgaatatc gtgcgcaacg aatcgttcac cgattcacgg ctgcgtctgc
      961 aggtgtccgt gcaggtggcc tacggcaccg accttgagca agcgatgcgc ctgatggtcg
     1021 aggcggcgcg ggcgcaaccg cgagtgctcg acgaccccgc gccgcaagcg ctgttgacgg
     1081 cttttgccga cagcggcatc aatctcgatc tcggcttctg gattgccgac ccgcaggaag
     1141 gaacaggcgg cgttcgttcg gcgatcaacc tgtcgatctg gcgctcgttt tgcgagcagg
     1201 gtatcgagat tcccttcccg cagcgcgagg ttcgcctgat cggcgggctg ccgcccgtgg
     1261 cgactgcgcc ggcggagccg gcggctcctg cagtggtccc gggattacag ccagtggctt
     1321 agccacgatc ttcaaccgcg attcacgccg aacgattcga ccggaaaagt gcgagatgag
     1381 cgccagcgca cgctgcgctc tgccaaaatc cggccgcgcg tcaagcgccg cccgcagcgc
     1441 gctgacggcc tgctgcggac ggcgcaggcg gaacagcagc aggccgaggt cgtaccaggc
     1501 gccggcgagc gtcgatccgt ggccgctcgc cgccggcagg cactgcgccg aggcttgcag
     1561 actggcgtcg gtagcggccg tcggctgctc gcgggggaga tcaccggccg ccgctgcaaa
     1621 aacctgcagg gcctgcgcgc ggcgcctggt gcggtaatag accaggccga ggaactgcag
     1681 cgatacgctg tcgtccggga tcgcctgcag gatgctgcgc gcgatccact ccgcctgcgc
     1741 cagatcgccg ctctgataga acgctcccag aaggtcgaac agggcattga tcatcgcatc
     1801 aaccgagctc gaccggcacg aagatcttc
//
LOCUS       DAOSIT010000508         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55267_length_1829_cov_8.470688, whole genome shotgun sequence.
ACCESSION   DAOSIT010000508 DAOSIT010000000
VERSION     DAOSIT010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55267_length_1829_cov_8.470688"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1031)
                     /gene="ispE"
                     /locus_tag="PLL14_10485"
     CDS             complement(171..1031)
                     /gene="ispE"
                     /locus_tag="PLL14_10485"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOG04398.1"
                     /translation="MNSTSQTWDWQSEYPAPAKLNLFLHVVGRRADGYHLLQTVFRFL
                     GHGDSLYFAPRADGAVRLATPLPGVPAEDDLTVRAARLLQAATGCRAGVDIRIEKRLP
                     LGGGLGGGSSDAATVLLALNHLWRLAVPRSRLQEIGLALGADVPVFLFGHNAFAEGIG
                     ETLQAVELPPCWYVVLEPPVQIPTAAIFGARELKRDSLPIAPREWHPGFGGNDLEAVA
                     CARFPTVAEHLTCLSALSPARMSGSGACVFAEFATRQQAEAALRQLPRGIRGWVAAGL
                     AEHPLLGLTN"
     gene            complement(1037..1642)
                     /gene="lolB"
                     /locus_tag="PLL14_10490"
     CDS             complement(1037..1642)
                     /gene="lolB"
                     /locus_tag="PLL14_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HOG04399.1"
                     /translation="MGTDLYLFSGGRSLACLLGVLGLSACTVLPPPAGGALPMTRTGL
                     HDFALSGRFALRQDGQSHVGRMNWRHDDSRNELLLSSPLGQGMAEMVSDASGARLIAG
                     DGKTYAAANADELLQSVLGYPLPLSRLVDWLRGNNPGGGRMALDGHGRPLRLLDEDWR
                     IDYEYDSDDPQALPGRLFVEREGGFELRLRIDEWSPLFPAN"
BASE COUNT          342 a          603 c          593 g          291 t
ORIGIN      
        1 cttcgaatgc cggaatcaaa atccggtgcc ttaaccaact tggcgactcc ccagctgtcg
       61 ctcgcatcgc ctggatggcg aacgaggcgc gcattatacc ggggattttt caggaaaaga
      121 agctccagtg ccgtttgcgg tgaacaaatc cggcgatgga actggcgcag tcagttcgtg
      181 aggccgagca ggggatgctc cgccaagccg gcggccaccc atccacgtat gcccctgggt
      241 aactgccgca gcgctgcctc ggcttgctgg cgggtggcaa actcggcgaa aacacaagcg
      301 cctgacccgc tcatccgggc cggcgaaagc gccgacaggc aggtcagatg ctcggcaacg
      361 gtcggaaagc gggcgcaggc taccgcttcg aggtcattgc caccaaaacc cgggtgccac
      421 tcgcggggcg cgatcgggag cgaatcgcgc ttgagttcgc gggcgccaaa gattgctgcc
      481 gtcgggatct gtacgggtgg ctcaaggacg acataccagc acggcggcag ttcgacagcc
      541 tgcagtgttt cgccgatgcc ctcggcaaaa gcgttgtgcc cgaagaggaa gaccggcaca
      601 tcggcaccca gggcgagacc gatctcctgc aggcgtgaac ggggcacagc aaggcgccag
      661 agatggttga gggccagcaa caccgtcgcc gcatccgaac tgccacctcc gaggccgcct
      721 ccgagcggca ggcgtttttc gatgcggatg tccacgccag cgcgacaacc cgtcgccgcc
      781 tgcaaaagcc tggcggcgcg aaccgtcaga tcgtcttcgg ccggaacacc cggcagcggc
      841 gtggccagtc gcaccgcgcc gtccgcgcgc ggtgcaaagt acaggctgtc gccatgtccg
      901 agaaagcgaa agacggtctg cagcaggtgg tagccatcgg cgcgcctgcc gacgacatgc
      961 agaaacagat tgagcttggc cggcgccgga tactcgctct gccagtccca ggtctggctg
     1021 gtggaattca ttgggctcag ttcgcgggga aaagagggct ccactcgtcg atgcgcaggc
     1081 gcaactcgaa gccgccctcg cgctcgacga acagacgacc gggcagggcc tgcgggtcgt
     1141 cgctgtcgta ctcgtagtcg atgcgccagt cttcgtcgag caggcgcagc ggacgtccat
     1201 gcccgtccag cgccatcctg ccgccacccg gattgttgcc acgtagccag tccaccagcc
     1261 tgctcaaggg caagggatag ccgagcaccg actgcagcag ttcgtcggcg ttcgccgccg
     1321 cataggtctt gccatcgccg gcgatcagac gcgcgccact ggcgtcgctg accatctcgg
     1381 ccatgccctg ccccagcggt gacgacagga gcagttcatt gcggctatcg tcatggcgcc
     1441 agttcatccg tccgacatgg ctctgtccat cctggcgcag tgcgaaacgc ccgctcagcg
     1501 caaaatcatg caagccggta cgcgtcatgg gcaatgcgcc accggccggc ggcggcagga
     1561 ccgtgcaggc gctcagccca agcacgccga gcaggcacgc gagcgagcga ccaccactga
     1621 acaggtacag gtcggtgccc aatacggcgg gccttgtgct cagggggcaa atcgcctgac
     1681 ggcttcggtc aggacttcgt tggtggggtt cttctgctgc gcctcgcgca agctgcgttg
     1741 cgcttcttcc ttgcggccta gcatccacaa cacttcaccg aggtgtgccg caatttccgg
     1801 gtcggggcgc aggccgtgcg cgcgctgca
//
LOCUS       DAOSIT010000509         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55268_length_1829_cov_8.381060, whole genome shotgun sequence.
ACCESSION   DAOSIT010000509 DAOSIT010000000
VERSION     DAOSIT010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55268_length_1829_cov_8.381060"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLL14_10495"
     CDS             complement(<1..1233)
                     /locus_tag="PLL14_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOG04400.1"
                     /translation="MRTRDFSAGEAMDVDLRFSDKEITAWGGMGLMKRMLDHLGFKAA
                     LADCGLPQPGSNRGYRPEQLILQFMLSVWCGANRFEHGEVTRHDPVLKRLFGFARMAN
                     FKAIMRLLGKFTQSRNESVMDHLYRWLFGKIAITGLTLDLDSTVMTRYGVQEGAVRGY
                     NPAKRGRASHHPLMAFVADLRMIANCWLRPGNSRSANNAQAFLSNTLHRLGDKAVCLL
                     RADSGFSDSAFLDHLDTLPMHYLIALRQNQPLQRALVGAEGWWPLTGDDGHPVPGIEL
                     TRFAYQPEAWSVPRWVIGIRQHIARRTDPKGKTLNLFADDPVIGQWRFSALTTDLDLA
                     AEMIWRMYRGRADCENRIKELKYDFAADSFNMKDFWATEAALNFVMLAYNLMSLFRQV
                     LLKTNGVKSSSGPVQHTLK"
     gene            complement(1280..>1829)
                     /locus_tag="PLL14_10500"
     CDS             complement(1280..>1829)
                     /locus_tag="PLL14_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04401.1"
                     /translation="AEANETYIADLIAGGLYQLVLTGSADQVGRYTLALTAGAPSPVI
                     DSLPVAAPLASQVLFDTHKGSKNAGVNLVNGDHLLIEGKIADDSVSAVNNEFTFTSTA
                     EALSAGIEWIVGQPDNLERTIGVNVDLFDSNDLLVASDTFVGLQDEQGFSQLAASGIG
                     PGTYTLRFTRDFPLARISNGSH"
BASE COUNT          394 a          545 c          524 g          366 t
ORIGIN      
        1 tttcagggta tgttgtacgg ggccggaaga cgacttcacg ccgttggttt taagcaaaac
       61 ctgacgaaac agactcatca ggttataggc cagcatcacg aaattgagcg ccgcttcggt
      121 cgcccagaag tccttcatgt tgaaactgtc cgccgcaaaa tcgtacttga gttccttgat
      181 ccggttctcg caatcggcgc gcccgcgata catgcgccaa atcatttcgg cggcgagatc
      241 cagatcggtc gtcaaggccg agaatcgcca ctggccaatg accggatcat cggcaaacag
      301 attcagggtc ttgccttttg ggtcggtgcg ccgagcgatg tgctgacgta tcccgatcac
      361 ccagcgcggt acagaccacg cctccggctg atacgcaaaa cgcgtcagtt cgatcccggg
      421 taccggatgg ccatcgtcac cggtcagcgg ccaccaaccc tcggcaccga ccaaggcccg
      481 ttgcaagggc tggttctggc gcaaggcgat gaggtaatgc atcggcaggg tgtcgagatg
      541 atccagaaag gcgctgtcgg agaagccact gtctgcacgg agcaggcaca cggccttatc
      601 gcccagacga tgcagggtat tggacagaaa agcctgcgca ttgttggccg aacggctgtt
      661 gcccggtcgt aaccaacagt tggcgatcat gcgcaggtca gcgacaaagg ccatcagcgg
      721 gtgatggctg gcgcgaccgc gtttggccgg gttgtacccc cgcaccgccc cttcctgaac
      781 gccgtagcgg gtcatgacgg tggagtccag gtccagggtc agcccggtga tcgcgatctt
      841 cccgaacagc cagcggtaga gatgatccat gaccgactca ttgcggcttt gggtgaattt
      901 gcccaacagc cgcatgatcg ccttgaagtt ggccatgcgg gcaaagccga acaggcgttt
      961 caagaccggg tcgtggcgcg tgacttcgcc atgttcgaag cgattggcac cacaccacac
     1021 cgagagcatg aattgaagga tgagttgctc cggacgataa cctcgattgc tccccggttg
     1081 cggtagccca caatccgcca gtgcggcctt aaatcccaag tgatcaagca tccgtttcat
     1141 gagccccatg ccgccccagg cagtgatctc tttgtcgcta aaacgaaggt caacatccat
     1201 cgcttctcct gcactgaaat cgcgagtcct tatcttgacc ggaaaacgcc ctgaacacta
     1261 ttgcaatcag ctacttgcgc taatggcttc cattagaaat ccgggctaat ggaaaatctc
     1321 gggtaaagcg caacgtgtag gtgcccggcc cgatgccaga cgcggccaat tgcgaaaacc
     1381 cctgttcgtc ttgcaggccg acgaaggtgt cgctggccac cagcaggtcg ttcgaatcga
     1441 acagatcgac gttgacgccg atcgttcgct ccagattgtc cggctgaccg acgatccact
     1501 cgattccggc tgacagcgcc tccgccgtac tggtaaacgt gaactcgttg tttactgcgc
     1561 tgacggaatc gtcggcgatt tttccctcga tcaggagatg gtcaccattc accagattga
     1621 cgccggcgtt cttcgagccc ttgtgcgtat cgaagagaac ctgacttgcc aggggcgccg
     1681 caacgggcag cgagtcgata accggggacg gcgctccggc agtcagcgcc aaggtatatc
     1741 gaccgacctg atcggcgctt cctgtcaaga ccaactgata cagtccgccg gcaatcaggt
     1801 cggcgatgta ggtctcgttc gcctccgcg
//
LOCUS       DAOSIT010000510         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55489_length_1824_cov_10.466365, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000510 DAOSIT010000000
VERSION     DAOSIT010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55489_length_1824_cov_10.466365"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PLL14_10505"
     CDS             complement(<1..1041)
                     /locus_tag="PLL14_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOG04402.1"
                     /translation="MTNPTIRFIRRGQVVTLVNVPPSRTLLEVLREDLGSTGTKEGCG
                     EGDCGACTVVLGEAVEGRIEYRAINACIRLAHSIDGLALWTAEDLASDPLIGPSAPKP
                     SRANGAAGLHPVQEALVQCHGSQCGFCTPGFAMSLFGLYQTKAPGAPVTRDEAASALS
                     GNLCRCTGYRPIVDAAVSMDRFPVQRVNEAELLAKLEHLKTLPRWGSADFDPDSIYMT
                     PNDLPQLLALRRQHPEAQIVAGCTDVGLWVTKQHRDFAKVLDVTRVAELRRVERYPHH
                     IAIGAAVTLADAFDALVADRPQLQSFAHRFAGLPVRNSGTLGGNVANGSPIGDSMPLL
                     IALGANVVLMSQR"
     gene            1127..>1824
                     /locus_tag="PLL14_10510"
     CDS             1127..>1824
                     /locus_tag="PLL14_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG04403.1"
                     /translation="MRDQTLFDKIDLHLIRVLHTVLTERSVSKAAIRLGMYQPAVSAS
                     LKKLRELAGDPLLVRSGAGMVPTDAGLRMLAPSASILRAAESMFSDARGFEPSSARQT
                     FRIAASDFLDPLFLPQLVAQIKQQAPHCVIEILPLSAESDYRARLAQGDMDVVIGNWL
                     KPPDDLHLGRLFGDEVVCLVARDHPAVRRSSQGGWDAASWLAAEHIAPTPTHPGARGV
                     IDEHLDSLGLQRNIT"
BASE COUNT          289 a          610 c          628 g          297 t
ORIGIN      
        1 gcgctggctc atcagcacca cgttggcgcc cagggcgatc agcaagggca tcgagtcgcc
       61 gatgggcgag ccgttggcca cgttgccgcc cagcgtgccc gagttgcgca ccggaaggcc
      121 ggcaaaacga tgggcaaagc tctgcagctg ggggcggtcg gccaccagcg cgtcgaaagc
      181 gtccgccagc gtgacggcgg cgccgatggc gatgtggtgc ggatagcgct cgacccggcg
      241 cagctcggcg acacgagtga cgtcgagcac cttcgcgaaa tcgcggtgct gcttggtgac
      301 ccacagtccc acatcggtgc atccggccac gatctgcgct tcggggtgct gacggcgcag
      361 cgccagcagc tggggcaggt cgtttggggt catgtagatc gaatctggat cgaaatcggc
      421 acttccccag cgtggaaggg tcttgagatg ctccagtttt gcgagcagtt cagcttcgtt
      481 gacgcgctgg accgggaacc ggtccatgga caccgcggcg tcaacgatgg gccggtagcc
      541 ggtgcagcgg cacaggttgc cggacagggc cgacgccgcc tcgtcgcggg tgacgggcgc
      601 gccgggtgcc ttggtctgat agagcccgaa caggctcatg gcaaagcccg gcgtgcagaa
      661 gccgcattgc gagccgtggc actgcaccag cgcctcctga accgggtgca aacctgctgc
      721 gccgttcgct cgcgacggtt tgggggcgct gggtccgatc agcggatcgc tggccaggtc
      781 ctcggccgtc cacagcgcca gcccgtcgat ggagtgggcc aggcggatgc aggcgttgat
      841 ggcacggtat tcgatgcggc cctccaccgc ctcgccgagc accacggtgc aggcgccgca
      901 gtcgccttcg ccacagccct ccttggtccc ggtgctgccg aggtcctcgc gcaggacttc
      961 cagcagggtg cggctgggtg gtacattgac cagcgtcacg acctggccgc ggcggatgaa
     1021 ccggatggtt gggtttgtca tgtctttgtt ccttgtgcgc gagggtagag cgatccccgc
     1081 ccccgcgcca taagctcccg catatgagtt gaatgcacgc accggtatga gagaccagac
     1141 ccttttcgac aagatagacc tccatctcat aagggtcctg cataccgtgt tgaccgaacg
     1201 cagcgtgtcg aaagccgcca tccgcctggg catgtaccag ccggcggtga gcgcgtcgct
     1261 gaaaaagctg cgcgagctcg ccggggatcc gcttctggtg cgctcaggcg ccggcatggt
     1321 gcccaccgac gccggcttgc gcatgctcgc ccccagcgca agcattcttc gtgccgcgga
     1381 gtcgatgttt tccgacgcgc gcgggttcga gcccagcagc gcgcggcaga cttttcgcat
     1441 cgccgccagt gatttcctcg acccgctgtt ccttccgcaa ctcgtggcgc agatcaagca
     1501 gcaggcgccc cactgcgtca tcgagatcct gccgctgtcg gccgagagcg actaccgcgc
     1561 gcggctggcg cagggcgaca tggacgtggt catcggcaac tggctcaagc cgccggacga
     1621 cctgcacctg ggccggctgt tcggcgacga ggtggtctgc ctggtggcgc gcgaccaccc
     1681 ggccgtgcgc cgcagcagcc agggcggctg ggatgcggcc agctggctgg cggccgagca
     1741 catcgcgccc acgcccacgc acccgggcgc gcgcggcgtg atcgacgagc acctcgactc
     1801 cctgggcctg cagcgcaaca tcac
//
LOCUS       DAOSIT010000511         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55825_length_1816_cov_7.499716, whole genome shotgun sequence.
ACCESSION   DAOSIT010000511 DAOSIT010000000
VERSION     DAOSIT010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55825_length_1816_cov_7.499716"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1816)
                     /locus_tag="PLL14_10515"
     CDS             complement(<1..>1816)
                     /locus_tag="PLL14_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG04404.1"
                     /translation="TFGRKDLSIADREYFRQIRNRPDRELHFSETLNARTTGTPSIVA
                     YRAILNRSGAFLGLVITPIDLRHFSREFSELQVGEQGMVSIRRSDDSRLVVRWPEAPE
                     EINKPADRTPPYLSIQSGKTQGIVRYMGQVDRVDRIFAFQKVSAYPFYVLVGRAVHEQ
                     FAAWRDTALISSALTVAGLLVLGGLLRRLKRSGAILRDSETRYRAIIEAQQDAVCRSL
                     PDTTLTFANHEYRRLFAGGEVEVLGRRWIEFVPEEERVAVLEQHARLSQAPDILEYEH
                     SVVFADGSVRWFHWAEVSLFDAQGQCTEIQSAGRDVTEQRQAEEALRSSEQRFRDIAF
                     TSADWIWEMDAQGRYTFASDNVMAILGHAPAELTGRTPFDLMPPQEATRVRGIFQDLA
                     ARKAPFSDLENLCRHHDGRLITLLTSGVPILSPTGELLGYRGTARDISARKQAEQALH
                     DREEVMSAIVSQAVDAIELTDLETFRFIEFNDASCRLLGYTREEYARLTVFDIQAELP
                     RDQTHALVAGARVGEERHFEARHRRKDGSVIEVQVSMRFIELHGRFCCVAIWRDIGER
                     KRVLVELERHRQHLEERVVARTAELAAAKEAAEQANLA"
BASE COUNT          317 a          569 c          559 g          371 t
ORIGIN      
        1 ttcgccagat tcgcctgttc agccgcctcc ttggcggctg cgagttcggc ggtgcgggca
       61 acaaccctct cctcaaggtg ctggcgatga cgttccagtt cgacgagcac ccgcttgcgc
      121 tcgccgatgt ctctccagat ggcaacgcag caaaagcgtc cgtgcagttc aatgaagcgc
      181 atgctgacct gaacctcgat cacgctgccg tccttgcgcc gatgcctggc ctcgaaatga
      241 cgttcttcgc cgaccctggc accggccacc agcgcgtgtg tctgatccct tggcaattcg
      301 gcctggatgt cgaagaccgt gagccttgca tactcctcac gggtgtagcc gaggaggcgg
      361 caggaagcat cgttgaactc gatgaagcgg aaggtttcca ggtcggtgag ttcgatcgca
      421 tccaccgcct gggagacgat cgcgctcatc acttcttccc gatcgtgcag ggcctgctcg
      481 gcctgtttgc gcgcactgat gtcccgggcg gtgccacggt aaccgagcag ttcgccggtc
      541 ggcgacagga tggggacgcc gctggtcagc agcgttatca gcctcccgtc gtgatgtcgg
      601 cagaggttct ccagatcgct gaaaggcgcc ttgcgtgcgg ccagatcctg gaagatgccg
      661 cgcacccgcg tggcttcctg tggcggcatc aggtcgaagg gggtgcggcc tgtcagttcc
      721 gccggcgcat gaccgagtat ggccataaca ttgtccgagg caaaggtgta gcgcccctgc
      781 gcatccattt cccagatcca gtcggcgctg gtgaaggcga tatcgcgaaa gcgttgttcg
      841 ctgctgcgca gcgcttcctc ggcttgtctt tgctcggtga catcccgtcc agccgactga
      901 atctcggtgc attgcccctg cgcgtcgaag agcgatactt cagcccaatg aaaccagcgc
      961 acactgccat cggcgaatac caccgagtgc tcgtattcga ggatgtcggg tgcttgcgac
     1021 aaacgcgcat gttgttccag gacggctacc cgttcctcct cgggaacgaa ttcgatccac
     1081 cgtcggccga gcacctcgac ttcgccgcca gcgaacaggc gacgatactc gtggttggcg
     1141 aatgtcagcg tggtgtcggg caaggatcga cagaccgcat cctgctgcgc ctcgatgatg
     1201 gcccggtaac gtgtttcgct gtcgcgcagg atggcgccgc tacgcttcaa ccgacgcagc
     1261 agaccgccca gcacgagcag tccggcgact gtcagcgccg acgagatcag tgccgtgtcg
     1321 cgccaggccg caaactgttc gtgaacggca cgaccgacca gtacgtagaa cgggtaggcg
     1381 ctgactttct gaaaggcgaa tatgcgatcc acccggtcga cctggcccat gtaacggacg
     1441 atgccttgcg tcttgccgct ctggatgctc agatacggtg gcgtccggtc ggccggcttg
     1501 ttgatttctt cgggggcctc tggccagcgc acgaccaggc gactgtcgtc gctcctgcgg
     1561 atactcacca ttccttgctc tccgacctgc agttcggaaa actcccggga gaaatggcgc
     1621 aggtctatcg gcgtgataac cagccccagg aatgctccac ttcggttcag gatggcccgg
     1681 tatgcgacga tgctcggcgt ccccgtggtt ctggcgttca gcgtctccga aaaatgcagt
     1741 tcgcgatccg ggcgattcct gatttgtcgg aagtactcgc ggtcggcaat gctcaggtcc
     1801 ttgcggccga aggtgt
//
LOCUS       DAOSIT010000512         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_55907_length_1814_cov_9.718590, whole genome shotgun sequence.
ACCESSION   DAOSIT010000512 DAOSIT010000000
VERSION     DAOSIT010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55907_length_1814_cov_9.718590"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>1814
                     /locus_tag="PLL14_10520"
     CDS             172..>1814
                     /locus_tag="PLL14_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HOG04405.1"
                     /translation="MTWQMNVDPLGNIGLSALVAAIPIFFLFWALAIKRMKGQFAAMG
                     GTGLAILIAVFVYKMPVDLAALATLYGGAFGLFPVCWIVITALFIYNMSVKTGQFEVI
                     KNSLASISDDRRLQALLIAFSFGAFIEGAAGFGTPVAITAAMLAGLGFNPLYAASICL
                     LANTAPVAWGAIGIPIVVGGQVAGIPDFALSQMVGRTLPIVSVFIPLYLVVVMSGWKK
                     GLEVWPACLVSGGSFAIAQFFSANSPVTAPLLPDIIASLASIVCTVAFLKVWHPKESW
                     HFADEPRSLGKQALKFTGGQIFRAWAPFIILSLFVCAWGIKPVNQALNQTTKMIFDYA
                     IPISGLDKMVIDQVGKPKPAVYNFNFLSAAGTAILFAWFFSIMVMGASLKTAAGVAGD
                     TLKTLKWPIVTIATILGFAYIMNFSGMAITLGYAFATTGAAFPFFASLLGWLGVFMTG
                     SDTSTNALFGKLQAVTAEKLAIDPVITMSANTCGGVCGKMISPQSIAVATGATGLVGR
                     ESEIFRFTFKHSIFLVVIIGILHMLWTYVFPGIVPAYVKP"
BASE COUNT          305 a          588 c          518 g          403 t
ORIGIN      
        1 cgaccaaccg cacctgcgaa atcggcctca ccgccgaaac cggccgcccc taccgctcca
       61 ttgcttacct gctcgaggag tgcagtcgta cggaaccccc gggagcctgc tgagccctgg
      121 caaccgtggt cacaacagcc acatgcgtgg cttattacaa aggagactcc catgacctgg
      181 caaatgaatg ttgatccgct cggcaacatc ggattgtccg cgctggtcgc ggcaatcccg
      241 atcttcttcc tgttctgggc actggccatc aagcgcatga agggccagtt tgcggcaatg
      301 ggaggcaccg gactggcgat cctgatcgcg gtgttcgtct acaagatgcc cgtggatctg
      361 gcggcgctcg cgaccctgta cggcggcgcg ttcgggctgt tcccggtttg ctggatcgtc
      421 atcacggcac tctttatcta caacatgtcc gtgaagaccg ggcagttcga ggtgatcaag
      481 aactcgctgg cctcgatttc ggacgaccgg cgcctgcagg cgctgctcat cgcgttttcc
      541 ttcggtgcct tcatcgaggg tgccgccggc ttcggtaccc cggtggcgat taccgcggcg
      601 atgctggctg gccttggctt caacccgctc tatgcggcgt ccatctgtct gctggccaac
      661 acggcgccgg tcgcctgggg tgccatcggc attccgatcg tggtcggcgg gcaggtcgcg
      721 ggaattcccg acttcgcgct gagccagatg gtcggccgga cactgccgat cgtcagcgtc
      781 ttcattcccc tctatctcgt cgtcgtcatg tcggggtgga aaaaggggct ggaagtctgg
      841 cccgcttgtc tggtcagcgg tggctccttt gccattgcgc aattcttctc ggccaactcg
      901 ccggtcacgg caccgctgct tcccgatatc attgcctcgc tggcttcgat cgtctgcacg
      961 gtcgcctttc tcaaggtctg gcatccgaaa gagagctggc acttcgccga tgaaccgagg
     1021 agcctgggca aacaggcgct caagttcacc ggcggtcaga tcttccgcgc ctgggcaccc
     1081 ttcatcatcc tgtcgctctt cgtctgcgcc tggggtatca agccggtcaa tcaggcactc
     1141 aaccagacga ccaagatgat cttcgactac gccataccga tttccggtct cgacaagatg
     1201 gtgatcgatc aggtcggcaa gcccaagcct gccgtctata acttcaactt cctcagcgcg
     1261 gccggcacgg ccattctgtt cgcctggttc ttctcgatca tggtcatggg cgcttccctc
     1321 aagaccgccg cgggcgtcgc gggcgatacg ctgaagactc tcaaatggcc gatcgtgacc
     1381 atagcgacga tcctcggctt tgcctacatc atgaactttt ccggcatggc gatcacgctc
     1441 ggttacgcgt ttgccacgac cggcgctgcc ttccccttct tcgccagcct tctcggttgg
     1501 ctgggagtgt tcatgacggg ttccgataca tcgacaaacg ccctgtttgg caagctccag
     1561 gcggtcaccg ccgagaagct cgccatcgac ccggtgatca ccatgtcggc caacacctgc
     1621 ggcggcgtgt gcgggaagat gatctcgccg caatcgatcg ccgttgccac aggcgctaca
     1681 ggactggttg gtcgtgaatc cgagatcttc cggttcacat tcaagcactc gatattcctg
     1741 gtggtgatca tcggcatcct gcacatgctc tggacgtacg tgttccccgg catcgttccg
     1801 gcttacgtca agcc
//
LOCUS       DAOSIT010000513         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56071_length_1810_cov_8.342450, whole genome shotgun sequence.
ACCESSION   DAOSIT010000513 DAOSIT010000000
VERSION     DAOSIT010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56071_length_1810_cov_8.342450"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..>1810
                     /locus_tag="PLL14_10525"
     CDS             496..>1810
                     /locus_tag="PLL14_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOG04406.1"
                     /translation="MARRSSTELAFTALVIEGGLLAPDFLNRIAHLEAAAQSEADYDI
                     PRGLKLRDEIGRYWKIAQNLWQDFAARRERSDRDPWANSRDFLEPFCRQVLGFADLKR
                     VGQVAVGERSFPIGCAAGEGRVPLVFAAHDQPLDKPGSRHGDGTRRRSPFLLAQEVLN
                     ASHAVLWAVVSNGLKLRVLRDNASLTRPAYIEADLEAIFSEGLYPDFAALWLLAHATR
                     FGQAGAEPADCPLEHWRNTAQEDGIRVRDRLREGVAKALRVLGSGFLAHPANGELRRR
                     IESGELSTPIYFEQLLRLVYRFIFLATIEDRELVFAPDAGAEARARYQAGYSLARLRR
                     LAARRRSYDRHADLWQAISITFIGLASGQPALGLPALGGLFHTAQCPDLDAAQLDNQS
                     LLTALFGLCFFRDGAALSRVNYRDMDSEELGSVYESLLELVPELTL"
BASE COUNT          302 a          675 c          527 g          306 t
ORIGIN      
        1 tgctcgcaga ccaccgccgt gtgcgcgagg ccgcccgcgg cagcggcgaa taccgagtca
       61 cgcccagcct gccggtcgac gtgatggggg tcttcgtgct cgtgccggcg tagcgcgaca
      121 gctaatccgg acacatcaag agcatgaatt cattggaatt tcgggtcgct gtagcgttaa
      181 gcattgatcg ccatgcttaa tttgacagac caggatcacg ccccgggggc ttcccgctgt
      241 cccggaatgt tcgcgcaacc agcggtttta taagagattt tcgcgctaga ctttgtagtg
      301 taaaaaaagt tacacaaaac gaattcatga catcctggaa attttacggc cgtcgcgacg
      361 agctggaggc gatcaagctg ctcctgaccc ggggccgctg gtccttctgc acgatcagcg
      421 gtcgccgccg catcggcaag atatctctga tagcgctggc ttgccgtgcc aggaacaact
      481 gaaccgggaa cccgcatggc acgccgctcc agtaccgaac tcgccttcac cgccctcgtc
      541 atcgaaggcg gcctgctcgc gcccgacttc ctcaaccgca ttgcccatct cgaagccgcc
      601 gcgcagtccg aggccgacta cgacatcccg cgcggcctca agctgcgtga cgagatcggc
      661 cgctactgga agatcgccca gaacctctgg caggatttcg ccgcccgccg cgaacgcagc
      721 gaccgcgacc cctgggccaa cagccgcgac ttcctcgaac ccttctgccg tcaggtgctc
      781 ggttttgccg acctcaagcg cgtcggccag gtcgccgtcg gcgaacgcag cttcccgatc
      841 ggctgcgccg ccggcgaagg gcgcgtgccg ctcgtcttcg cggcgcacga ccagccgctc
      901 gacaagccgg gcagccggca cggcgacggc acgcgccgcc gttcgccctt cctgcttgct
      961 caggaggttc tcaacgccag ccacgccgtg ctgtgggccg tcgtcagcaa cgggctcaaa
     1021 ctgcgcgtcc tgcgcgacaa cgccagcctc acccggccgg cctacatcga ggccgatctc
     1081 gaagccatct tcagcgaagg cctttacccg gatttcgccg ctctctggct gctcgcccac
     1141 gccacgcgct tcggccaggc cggcgccgag ccggccgact gcccgctcga acactggcgc
     1201 aacaccgcgc aggaagacgg catccgcgtc cgcgaccgcc tgcgcgaagg cgtcgccaag
     1261 gccctgcgcg tgctcggcag tggctttctc gcgcatccgg ccaacggcga gttgcgccgc
     1321 cgcatcgaga gcggcgaact gagcaccccg atctacttcg agcaactcct gcgcctcgtc
     1381 taccgcttca tctttctcgc caccatcgaa gaccgcgaac tcgtcttcgc gcccgacgct
     1441 ggcgccgagg ctcgtgcccg ctaccaggcc ggctacagcc ttgcccgcct gcgccgcctg
     1501 gccgcccggc gacgcagcta cgaccgccac gccgacctct ggcaggcgat ctcgatcacc
     1561 ttcattggcc tcgccagcgg tcagccggcg ctcggtctgc cggcgctcgg tgggctgttc
     1621 cataccgcgc aatgcccgga cctcgacgcc gcccagctcg acaaccagtc gctgctgacc
     1681 gcgctcttcg gcctctgctt cttccgcgac ggcgccgcgc tctcgcgcgt caattaccgc
     1741 gacatggatt ccgaggaact cggcagcgtc tacgaaagcc tgctcgaact ggtgcccgaa
     1801 ctgaccctga
//
LOCUS       DAOSIT010000514         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56320_length_1804_cov_10.820469, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000514 DAOSIT010000000
VERSION     DAOSIT010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56320_length_1804_cov_10.820469"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /gene="fliW"
                     /locus_tag="PLL14_10530"
     CDS             complement(<1..194)
                     /gene="fliW"
                     /locus_tag="PLL14_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOG04407.1"
                     /translation="MKIDIEHFGLRDVPVDPDTVFTFAEGVAGFTHCTQFTIFHEEGS
                     NPRVFWLQSLDDAEVMFPIVA"
     gene            364..>1804
                     /locus_tag="PLL14_10535"
     CDS             364..>1804
                     /locus_tag="PLL14_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOG04408.1"
                     /translation="MPPSIETRVAQQIAERIAAEVACRPQQVLATAALLDEGATVPFI
                     ARYRKEVTGGLDDIQLRLLDERLTYLRELEDRRRSVLASIDEQGKLSPELAAAINAAD
                     TKQRLEDLYLPYKPKRRSKAQAAREAGLAPLAEALLANPLLAPEVEAERFLNADAGFG
                     DVRSVLDGARQILMEQFSEDAALLGELRSYLEAHGFVKSTLVAGKEIEAAKFQDYFAY
                     AEPLLAVPSHRALALFRGRNEGLLQLALVLDNELDDKANGPNPCESRIARRFGIVDQG
                     RPADRWLAETVRWTWRIKASLHLELELMNALRERAEAEAIRVFGANLHDLLLAAPAGK
                     HATMGLDPGLRTGVKVAVVDPTGKLLDTATIYPHEPRRDWDGALHTLAQLARRHQVAL
                     ISIGNGTASRETDRLVGDLIRQHADLHLGKIVVSEAGASVYSASEYASREFPDLDVSL
                     RGAVSIARRLQDPLAELVKIDPKSIGVGQY"
BASE COUNT          338 a          628 c          523 g          315 t
ORIGIN      
        1 gccacaatcg gaaacatcac ctcggcgtcg tccagcgact gtagccagaa caccctggga
       61 ttgctgcctt cctcgtggaa aatcgtgaac tgcgtgcagt gcgtaaaacc cgcaaccccc
      121 tcggcgaaag tgaatacggt atccggatca accggcacgt ctctcagacc aaagtgttca
      181 atatcgattt tcatgatgcc tccctgagcc acagcgggat gtcgctgccc aagactggca
      241 atttaaccct tttctcggtg cggcggcaat gcaatcggcc tggcgaccgt cggtgttctt
      301 gagttttgcc tgccgattct gcatagttcg gcctttttca aaccttccga cctgtcctcg
      361 ctcatgccgc catcgatcga aacccgtgtt gcccagcaga ttgccgaaag gattgccgca
      421 gaagtggcct gtcgccccca acaggtgctg gccaccgccg ccctgctcga cgaaggcgcc
      481 acagtgccgt tcatcgcccg ctatcgcaag gaggtcaccg ggggactcga cgacatccag
      541 ttgcgcctgc tcgacgaacg cctgacctac ctgcgcgaac tcgaagaccg gcgcagatct
      601 gtcctcgcct cgattgacga gcagggcaag ctgagcccgg agctggcagc ggcaatcaac
      661 gccgcggaca ccaagcaacg cctcgaagat ctttacctgc cgtacaagcc gaagcggcgc
      721 agcaaggcgc aagccgcccg ggaagccggt cttgcaccac tcgccgaagc cctgctcgcc
      781 aacccgctgc tggcccctga agtcgaggcc gaacgcttcc tcaacgccga cgccggcttc
      841 ggcgatgtca ggagcgtgct cgacggcgcc cgccagattc tgatggaaca gttttcggaa
      901 gacgccgccc tgctcggcga gttgcgcagc tacctcgagg cgcatggctt cgtgaaatcg
      961 acgctcgtcg ccggcaagga aatcgaggcc gccaagtttc aggactattt tgcctacgcc
     1021 gaacccctcc tggcggttcc ctcgcatcgc gccctggcgc tgttccgcgg ccgtaacgag
     1081 ggcctgctgc aactggcgct ggttctcgac aatgaactcg acgacaaggc gaatggcccc
     1141 aacccgtgtg aatcgcgcat cgcgcggcgc ttcggcatcg tcgaccaggg ccgaccggcc
     1201 gacaggtggc ttgccgaaac agtgcgctgg acctggcgca tcaaggcttc gctgcacctc
     1261 gaactggaat tgatgaatgc gctgcgcgag cgtgccgaag ccgaggcgat ccgggtgttt
     1321 ggcgccaacc tgcacgatct gctgctcgct gcacccgccg gcaagcacgc taccatgggc
     1381 cttgaccccg gcctgcgcac cggcgtcaag gttgcagtcg tcgaccccac cggcaaactt
     1441 ctcgacaccg ccacgatcta cccacacgag ccgcgccgtg actgggacgg cgcgctgcac
     1501 accctggcac agctcgcgcg caggcatcag gtcgccctga tcagcatcgg caacggcacc
     1561 gcctcgcgcg agaccgatcg tctggttggc gacctgatcc gccagcacgc ggacctgcac
     1621 ctcggcaaga tcgtcgtttc ggaggcaggc gcttcggtct attcggcttc cgaatacgcc
     1681 tcgcgcgaat tcccggacct cgacgtcagc ctgcgcggcg ccgtatcgat tgcccgtcgc
     1741 ctgcaggacc cgctggccga actggtgaag atcgacccca agtccatcgg ggtcggccag
     1801 tacc
//
LOCUS       DAOSIT010000515         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56590_length_1798_cov_7.653471, whole genome shotgun sequence.
ACCESSION   DAOSIT010000515 DAOSIT010000000
VERSION     DAOSIT010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56590_length_1798_cov_7.653471"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLL14_10540"
     CDS             complement(<1..322)
                     /locus_tag="PLL14_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG04409.1"
                     /translation="MPVADLPPQLQERVVCSIAAAVKYQVPANIVLAVAEKEAGKPGQ
                     WVLNTNGTHDVGPMQFNTAYLRDLARYGITPGDVAAAGCYAFDLAAWRLRQHINKDRG
                     DLWTR"
     gene            complement(341..925)
                     /locus_tag="PLL14_10545"
     CDS             complement(341..925)
                     /locus_tag="PLL14_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HOG04410.1"
                     /translation="MKNHCVFAPFALAIAAATAASAGAQEVLTGDTRLACEATLCLAA
                     STRPSECSPSLSRYFSIHKRKWSDTVRARANFLSLCPVSDQTPEMRSLVNAMANGAGR
                     CDAASLNVTLLVWNSWDADGGRVLINNQMPGYCVAYTGHQYTDLGDLAPRYVGTPERG
                     GYWVDARDYNAAVARYNDRIAAEDSRRRNEEWYR"
     gene            complement(940..>1798)
                     /locus_tag="PLL14_10550"
     CDS             complement(940..>1798)
                     /locus_tag="PLL14_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HOG04411.1"
                     /translation="AQLFTMVLLVGIGKSFVDQYYAGMSAGISLKELGVMMIVAVVMF
                     ALVTKLPALIGGLAMGGSVHTMGSGLGTGAAMAAVGMASAAVGVAGAAIAAGAASMAG
                     GTQALMAAFSKASQDVADGSDMLASLAGGTSDGGRSSGGSGASPLAAAMDSSSAGGGS
                     SGDGSSHSGRSDTRQAIAKRGGTSGEVQSGGRASVVAKVGKVAAGTVANLAQGTWDVA
                     KAKAGEMGDAAMDRIGETTGGKIAAAIKARGDQKPVFGSDSLAKADDQSVDAEAEIAA
                     FRDRAARSS"
BASE COUNT          297 a          636 c          536 g          329 t
ORIGIN      
        1 cccgcgtcca gaggtcgccc ctgtccttgt tgatgtgctg ccgcagccgc caggcggcga
       61 gatcgaaggc atagcaaccg gctgccgcta cgtcgccggg cgtgatgccg tagcgagcca
      121 ggtcgcgcag gtacgccgtg ttgaactgca tcggccccac gtcgtgcgtg ccgttggtgt
      181 tgagcaccca ctgacccggc ttgccggctt ccttttccgc cactgcaagc acgatgttcg
      241 cgggaacctg gtacttcacg gcggctgcga tcgagcagac gacgcgttcc tgaagctggg
      301 gcggaagatc ggcaacaggc atgagctttc tgctttcctc ctaccgatac cactcttcat
      361 tcctgcgccg gctatcttcg gcagcaattc gatcgttgta ccgagccacg gcagcgttat
      421 agtcgcgcgc atcgacccaa tagccgcctc tttccggcgt cccgacatag cggggagcca
      481 ggtcgccgag gtcggtgtac tggtgcccgg tgtaggccac gcaatagccc ggcatctggt
      541 tgttgatcaa caccctgcca ccgtcagcgt cccagctgtt ccacacgaga agcgtcacat
      601 tcagtgaagc cgcgtcgcag cggcccgcgc cgttcgccat ggcgttcacc agcgagcgca
      661 tttccggagt ctgatccgac accgggcaca ggctcaggaa gttggcgcgt gctcgcacgg
      721 tgtccgacca cttccgttta tggatactga aatagcgcga gagcgacggc gagcactcgc
      781 tcggacgggt gctcgccgcc aggcacaggg tggcctcaca cgccaggcgg gtatcgccgg
      841 tcagcacctc ctgggcaccg gcggaggccg cggttgcggc ggcgatcgcc agggcgaacg
      901 gagcgaaaac gcagtggttt ttcatcgggg aggctccttt caagaagatc tggcggcgcg
      961 gtcacggaat gcggcgattt ccgcttcggc gtccaccgat tggtcgtctg cctttgccag
     1021 gctgtcgcta ccgaatacgg gcttctgatc gcccctggcc ttaatggccg cagcgatctt
     1081 gccgcccgtc gtctcgccga tgcgatccat cgcggcatcc cccatttcgc cggctttggc
     1141 ctttgccacg tcccaggtgc cttgcgccag attcgccacg gtacctgccg cgaccttgcc
     1201 gaccttggcc actaccgaag ctctgccgcc tgactgcact tcacccgacg ttccaccccg
     1261 cttggcgatt gcctgtctgg tgtccgatct gccggaatgt gaactgccgt cgccagacga
     1321 cccgccgccg gctgaactgc tatccatcgc cgccgccagc gggcttgcgc cgctaccgcc
     1381 cgacgatcgg cccccatccg acgtaccgcc agccaggctc gcaagcatgt cgctaccatc
     1441 ggctacgtct tgcgacgctt tggagaaggc tgccatcaag gcttgcgtac cgccagccat
     1501 gctcgccgcc ccggcggcaa tggctgcgcc ggcgacacca acggccgcac tggccatgcc
     1561 gaccgccgcc atcgccgcgc ccgtaccgag gccactgccc atggtgtgca cgctgccacc
     1621 catcgccagg ccgccgatca acgcaggcag cttggtgacg agcgcaaaca tcaccacggc
     1681 gacgatcatc atcacgccca actccttcag gctgatgcct gcactcatcc ccgcgtagta
     1741 ctggtcgaca aaggacttgc cgatgccgac cagcaggacc atggtaaaga gctgcgcg
//
LOCUS       DAOSIT010000516         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56688_length_1796_cov_6.574957, whole genome shotgun sequence.
ACCESSION   DAOSIT010000516 DAOSIT010000000
VERSION     DAOSIT010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56688_length_1796_cov_6.574957"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLL14_10555"
     CDS             <1..300
                     /locus_tag="PLL14_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG04412.1"
                     /translation="ETRRQEFQRRRDFLAPALEGLGFHVPAKPDGAFYIYADCSALTG
                     NSEIFAHDLLESAGVAVTPGLDFGRHAPEKHLRLAYTTGVERLAEAVDRIRRFLG"
     gene            complement(449..1012)
                     /gene="ampD"
                     /locus_tag="PLL14_10560"
     CDS             complement(449..1012)
                     /gene="ampD"
                     /locus_tag="PLL14_10560"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOG04413.1"
                     /translation="MEVGSCRDGGWLERARHIESPNCDARPDGEVVSLVVVHAISLPP
                     GHFGGEGIVQLFTNRLDPTAHPYFAQISGLRVSAHFLLRRDGELIQFVSGRQRAWHAG
                     RSCWQGRERCNDFSLGIELEGCDDLPFEDAQYLGLVDLVTLLCAQYPIAAVVGHSDIS
                     PGRKTDPGPCFDWRRLGSIGNLPGKLR"
     gene            complement(1024..>1796)
                     /locus_tag="PLL14_10565"
     CDS             complement(1024..>1796)
                     /locus_tag="PLL14_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG04414.1"
                     /translation="APFVPVNCGAIPENLMESEFFGYRKGAFTGADRDREGFFQAATG
                     GTLFLDEVADLPLVMQVKLLRAIQEKKVRKVGHTAEEAVDVRIISATHRMLKDCVDSG
                     TFRQDLYYRLNVIELRMPPLRERDEDVAPLVEALLVRICGTQPPRLDPQAMTVLKAYS
                     FPGNVRELENILERATALCNHDRIVLDDLQLAPATVVNDSAGRDGETLDDYLNRVEKQ
                     AILDALAKTAFNRTAAARLLGVTFRSLRYRIERLGIEE"
BASE COUNT          367 a          583 c          521 g          325 t
ORIGIN      
        1 gagacgcggc gccaggagtt ccagcgacgg cgcgatttcc tggcacccgc gctcgaaggc
       61 ctgggtttcc acgtgccggc caagcctgat ggcgctttct atatctatgc cgattgtagc
      121 gccttgaccg gcaacagcga aatcttcgcc cacgacctgc tagagtcggc aggagtcgca
      181 gtcacccccg gcctcgactt cggtaggcac gcgcctgaaa agcacttgcg ccttgcctac
      241 accaccgggg tcgaacgact ggccgaagcg gtcgacagaa tccgccgctt tctcggctga
      301 acaccgaccg actgtggcac cgggatcggc aacgatcgct ggcgtgggca acacggtacg
      361 tgggtctcac agccacttgg cagcctcaga gtgccggttc cttcctgcaa ccaggcccac
      421 tgacacccgc ctcagcacgc cctagcgatc aacgtagctt ccctggcagg ttcccgatcg
      481 atccgaggcg ccgccagtcg aaacagggcc cagggtcggt cttgcgacca ggtgaaatat
      541 cgctgtgccc gacgaccgca gcgatcgggt attgcgcgca caacagcgtc accaggtcca
      601 ccaggccgag gtactgcgcg tcctcgaacg gcaagtcgtc gcagccctcc agttcgatgc
      661 ccagcgaaaa atcgttgcaa cgctcccgac cttgccagca cgagcgaccg gcatgccagg
      721 cgcgttgccg gccggaaaca aactggatca gttcaccatc gcgtcgcagc agaaaatgcg
      781 ccgaaacccg caagccactg atctgtgcaa agtaggggtg ggcagttggg tcgagtcgat
      841 tggtaaacaa ttgcacgatg ccctcgccac caaaatgccc aggcggcagg cttatcgcgt
      901 gtacgacgac cagcgatacg acctcaccgt ccgggcgggc atcacaattg ggcgattcga
      961 tgtgccgcgc gcgttcgagc cagccgccgt ctcggcaaga gccaacctcc atgcgcgaag
     1021 ggttcactcc tcgataccca ggcgctcaat gcggtagcgc aacgaacgaa aggtcacgcc
     1081 aagcaaccgc gcggctgcag tgcggttgaa agcggtcttg gccagagcat ccagaatggc
     1141 ctgtttctcg acacgattca gataatcatc aagcgtttcg ccgtcacggc cggcgctgtc
     1201 gttgactacc gttgccggcg ccagttgaag atcgtcgaga acgatccgat cgtggttgca
     1261 cagcgcagtc gcccgctcaa gaatgttttc gagctcgcgg acattaccgg gaaaactgta
     1321 ggccttcagc accgtcatcg cttgcggatc caggcgcggc ggctgggttc cgcagatacg
     1381 taccagcagc gcctcgacca aaggcgccac atcctcgtcg cgctctcgta atggtggcat
     1441 tctgagttcg atgacgttga gccggtaata gagatcctgg cgaaacgtcc cgctatccac
     1501 acagtccttg agcatccggt gagttgccga gatgatgcgt acgtcgaccg cctcttctgc
     1561 ggtgtgcccg accttgcgaa cctttttttc ctgaatcgcc cgtagcagct tgacctgcat
     1621 caccagcggc aggtcggcga cctcgtcgag aaaaagcgtg ccgccggttg ccgcctgaaa
     1681 gaaaccttcg cggtcgcgat ccgcacccgt gaatgccccc ttgcgataac cgaaaaattc
     1741 gctctccatg aggttttccg ggatcgcacc acagtttacc gggacgaatg gggccg
//
LOCUS       DAOSIT010000517         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56728_length_1795_cov_11.355747, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000517 DAOSIT010000000
VERSION     DAOSIT010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56728_length_1795_cov_11.355747"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLL14_10570"
     CDS             <1..301
                     /locus_tag="PLL14_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOG04415.1"
                     /translation="HYFIQLLVTDAGSQREVADFIANNSRTLDLRQLRVYRSSFGGKE
                     RLGVIYGDYASREQANAALATLGEISPSSRPYVRPVARLRSARLPEGSESKALRQ"
     gene            372..>1795
                     /locus_tag="PLL14_10575"
     CDS             372..>1795
                     /locus_tag="PLL14_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HOG04416.1"
                     /translation="MLLRILRLTGALLLPLLFGACTPTTLSNGPSTGHLQADSVARPQ
                     GAIPQPVQQSVYLPKPRAIGKTETYSVVVNNVQVHDLLFALARDARLNVDVHPGLTGL
                     VTLNAIDQTLPQLLSRIAKQVDMRFELDGPNLAVMPDAPYLKNYKVDYVNIARDVTAT
                     VSTNTQISTSSLATGSGGVAGSGNTSRIQIENKSRNRFWESLEKNLKDLLHETDKVFP
                     EGSTETVTEQTASQSTTGTGAASPPSAAARVAQAAVQTLAGSPNPATLQNSGATVVKR
                     MTFREAASVIANPEGGVITVRATSRQHEKVQEFLDRVMSSARRQVLIEATIVEVTLSD
                     GYQQGIEWSRLTQATDISITKPSLTTNVQNIVTPYVLKYDSKNPLGVLATVDLLRAFG
                     TVKVLSSPKLSVLNNQTATLKVSEDFVYFSVKQDTSTLGGTQGGTLTATTTTPQSVSI
                     GFFMSLTAQISDSGTVTLNVRPSIS"
BASE COUNT          382 a          557 c          533 g          323 t
ORIGIN      
        1 ccattatttc atccagttgc tggtgaccga cgccggcagc cagcgcgaag tggccgattt
       61 cattgccaac aacagcagaa ccctcgattt acggcaactg cgggtttatc gttcaagttt
      121 cggtgggaag gagcgactcg gggtcattta tggcgactat gcttcccgcg agcaggccaa
      181 cgccgcgctg gcaacgcttg gcgagatcag cccgtcaagc aggccgtatg tgcggccggt
      241 ggccaggttg cgctctgcac gtctcccgga aggatcggaa agcaaggcct taagacaata
      301 aaaatcatgt acttgtaagg aagttccggc gtgctatcat gactcaccca ttgactggat
      361 caagtttgag cgtgctcctg cgaatcctgc gtctcactgg cgccctcctt ctcccgctcc
      421 tgttcggagc ctgtacgccg accacgctca gcaacgggcc gtcgacggga catctgcagg
      481 ccgacagcgt cgccaggccg cagggcgcga ttccgcagcc cgtacagcag agcgtctatt
      541 tgcccaagcc ccgcgccatc ggcaagacgg aaacctacag cgtggtggtc aacaatgtgc
      601 aggtgcacga cctgctgttt gccctggccc gtgacgccag gctgaacgtg gacgtccatc
      661 cgggcctgac cggcctggtg acgctcaacg ccatcgacca gacgctgccg cagttgctca
      721 gccgcatcgc caagcaggtg gacatgcgct tcgagctcga cggcccgaac ctggcggtga
      781 tgcccgacgc gccctacctc aagaactaca aggtcgacta cgtcaatatt gctcgcgacg
      841 tcacggccac cgtgtcgacc aatacgcaga tttcgaccag ttcgctggcg accggcagtg
      901 gtggcgtcgc gggctccgga aatacctcac gcatccagat cgagaacaag tcgaggaacc
      961 gcttctggga gtcgctcgaa aaaaatctca aggatctgct gcacgaaacc gacaaggttt
     1021 tcccggaggg atcgactgaa acggtgaccg agcagaccgc ctcacagagt acgaccggca
     1081 ccggtgccgc cagcccgccc agcgccgcgg cacgtgtggc gcaggccgcc gtgcagacgc
     1141 tggccggcag tccgaatccg gcgacgctgc agaacagcgg cgccacggtc gtcaaacgca
     1201 tgaccttccg ggaagcggca tcggtgattg ccaatccgga aggcggggtg atcaccgttc
     1261 gggcgacctc gcgacagcac gagaaggtgc aggagttcct cgatcgggtg atgagcagcg
     1321 ctcgccggca ggtgctgatc gaagcgacga tcgtcgaagt gacactttcc gatggttacc
     1381 agcaaggcat cgaatggagc cggctgaccc aggcgaccga catcagcatc acgaaaccat
     1441 cgctgaccac gaacgtgcag aatattgtca caccgtacgt tctcaagtac gacagcaaga
     1501 accccttggg ggtgttggca accgtggatt tgctgcgcgc ttttggtacc gtcaaggtgc
     1561 tctccagccc aaagctttcg gtgctcaata atcagacggc aacgctgaag gtttccgagg
     1621 attttgtgta cttcagtgtc aaacaggaca cgtctacgct cggcgggacg caaggcggca
     1681 cgctgaccgc caccaccacg acaccacagt cggtgtcgat tggcttcttc atgagcctga
     1741 cggcgcagat cagcgatagc ggcacggtca ctttgaacgt tcggccgtcg atttc
//
LOCUS       DAOSIT010000518         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56781_length_1794_cov_9.811961, whole genome shotgun sequence.
ACCESSION   DAOSIT010000518 DAOSIT010000000
VERSION     DAOSIT010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56781_length_1794_cov_9.811961"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLL14_10580"
     CDS             complement(<1..1154)
                     /locus_tag="PLL14_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOG04417.1"
                     /translation="MSSGLIFALICAVIAVLYGAAMTKWILSLPAGNPRMQEIAKAIQ
                     EGASAYLAKQYTTIGLVGAVLFVLIWFALGKLMAIGFLIGAVLSGATGFIGMNVSVRA
                     NVRTAEAARSGISAALDVAFKGGAITGMLVVGLGLLGVAGYYAFLKGGGSDFQHSVEP
                     LVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYAVTVVATMVLSAMMLKGVVGANDNLIVYPLVLGGVSIIASIIG
                     CFFVKASDGGKIMNALYRGLAVAGVLALIAFYPVTTYLLGNGVTLADGTLITSMNIFW
                     AASVGLVLTGLLVWITEYYTGTDFSPVKRVAEASTTGHGTNIIAGLAVSM"
     gene            complement(1361..>1794)
                     /locus_tag="PLL14_10585"
     CDS             complement(1361..>1794)
                     /locus_tag="PLL14_10585"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOG04418.1"
                     /translation="DDCQAGYLFDGFPRTLPQAEAMKVAGVALDYVLEIDVADEEIIE
                     RMSGRRVHPGSGRTYHIRFNPPREAGKDDVTGEDLIQRDDDQEETVRKRLQVYHSQTR
                     PLLDYYRDWAASGDPGAPKCRRIAGVGSVDDIRKAAFEALQ"
BASE COUNT          384 a          575 c          523 g          312 t
ORIGIN      
        1 ttcatcgaaa cggccagacc ggcgatgatg ttggtcccgt ggccggtggt cgaagcttca
       61 gcgacgcgct tgaccggcga gaagtcggta ccggtgtagt actcggtgat ccacaccagc
      121 aatccggtga gtaccagccc gacggacgct gcccagaaga tgttcatcga ggtgatcagg
      181 gtgccgtcgg cgagggtaac gccgttgccg agcaggtagg tggtgaccgg atagaaggca
      241 atcagcgcca gcacgccggc cacggccagg ccgcggtaga gggcgttcat gatcttgccg
      301 ccatcgctgg ccttgacgaa gaaacagccg atgatcgagg caatgatcga cacgccgcca
      361 agcaccagcg gatagacgat caggttgtcg ttggcaccga cgacaccctt gagcatcatc
      421 gcgctgagca ccatcgtcgc gacgacggta accgcgtagg tttcgaagag gtcggcggcc
      481 atgccggcgc agtcgccgac gttgtcgccg acgttgtcgg cgatcaccgc cgggttgcgc
      541 ggatcatctt ccggaattcc agcttcgacc ttgccgacca ggtcggcacc gacgtcagca
      601 cccttggtga agatgccgcc acccagtcgg gcgaagatcg agatcagcga accaccgaag
      661 gccaggccaa ccagcggttc aacgctgtgc tgaaagtcag atccgccgcc cttgaggaac
      721 gcgtagtaac cagcgacgcc aagcaggccc agtccgacga ccagcatgcc ggtaatcgca
      781 ccgcccttga aggccacgtc gagcgccgca gaaatgccgc tgcgtgccgc ctcggcggta
      841 cgcacgttgg cgcgcaccga aacgttcatg ccgatgaatc cagtagcacc cgacaatacc
      901 gcgccgatca gaaagcctat ggccatcagt ttgcccaggg caaaccagat caatacaaaa
      961 agtacggccc cgacgaggcc gatggtcgtg tactgcttgg cgagataagc cgaggcacct
     1021 tcctggatgg ctttggcgat ttcctgcata cgtgggttgc cggctggcaa cgagagaatc
     1081 cacttcgtca ttgctgcgcc ataaagcacc gcaatgaccg cgcatatcag cgcgaagatc
     1141 aaacctgaag acataagcaa cctccctctc taaagtgacc caaacctccc ggagagtccc
     1201 cacgggccgg tctttcccta cagctgaacg acgaaacaaa caacgctaaa tcggcccccc
     1261 ggtcctgcgg cgctgtcacg gtgctggcaa aacgatacga tcggcgccac caaaactgcg
     1321 tcgtggcgcc gccgtggcat tactcgggga gtacgaccgg ttactgcaag gcctcgaaag
     1381 ccgccttccg gatgtcgtcg acgcttccga caccggcaat tctgcggcac ttcggcgccc
     1441 cgggatcgcc acttgcagcc cagtcccggt agtaatcgag cagcggcctg gtttgcgagt
     1501 ggtaaacctg tagccttttc ctgaccgttt cttcctggtc gtcatcgcgc tgaatcagat
     1561 cttcgccggt gacatcgtcc ttgcctgcct cccgtggtgg attgaagcgg atgtggtacg
     1621 tacgaccgga acccggatgc acccgacggc cgctcatccg ctcgatgatc tcctcgtcgg
     1681 caacgtcgat ctcgagaaca tagtcgagcg ccactccggc gaccttcatc gcctctgcct
     1741 gcggcagagt gcgcggaaaa ccgtcaaaca gataacccgc ctggcaatca tcct
//
LOCUS       DAOSIT010000519         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56821_length_1793_cov_13.895282, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000519 DAOSIT010000000
VERSION     DAOSIT010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56821_length_1793_cov_13.895282"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLL14_10590"
     CDS             complement(<1..320)
                     /locus_tag="PLL14_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOG04419.1"
                     /translation="MTFLRWSEHFVTHIDSVDRQHQGLVDLVNAAAPALSCGDPSDAD
                     AQVLIDRLAEYAMTHFREEEQLMRDAGIDGNYLDHHMRSHRSFVEEVALMRRQFDTDR
                     RLDGP"
     gene            complement(317..>1793)
                     /locus_tag="PLL14_10595"
     CDS             complement(317..>1793)
                     /locus_tag="PLL14_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOG04420.1"
                     /translation="YGGAIYESTRFVIPQDDGPALLGGIALDITRRHQLETRTRALLE
                     INEVGGSLPEREFLAHGLELAERLTGSRIGFLHFVNDDQETIELVTWTAGALQGCKAA
                     FDSHYPISQAGIWADCFRRKQPVTFNHYADYADKHGLPDGHAALQRLISVPVIEESAV
                     RMMIGVGNKPDDYNDVDLTTVQLLGNDLWRIVRRARAEASLARRLAEVTQLKERLEQA
                     HLQLLQSEKMSAIGQLAAGVAHELNNPIGFVHSNLGTLQKYVDDLLAIEAAYVRVEQL
                     PPPHSVAALEDARRLKVACDYEYIIDDLPKLIRESKEGLERVRRIILDLKDFSRSGEN
                     DWQWADLHACLDSTINIAWNELKYKADVERQYGELPNVRCLPSQLNQVFMNLLVNAAQ
                     AIEQRGRIVVRTASHDGQSVWVEIEDSGQGLSPEVQRRLFEPFFTTKPVGQGTGLGLS
                     ISFNIIEKHQGRIDVRSNPGHGTTFRITLPVNGSQHKEAKA"
BASE COUNT          296 a          526 c          594 g          377 t
ORIGIN      
        1 gggccgtcga ggcgacggtc ggtatcgaac tggcggcgca tcaaggccac ctcttcgaca
       61 aagctgcgat ggctgcgcat atggtgatcg aggtagttgc cgtcgatgcc ggcgtcgcgc
      121 atcagttgct cttcctcacg aaaatgcgtc atcgcgtatt cggcaaggcg atcgatcagc
      181 acctgcgcgt cggcgtcact cggatcaccg cacgacaacg ccggcgcagc ggcattgacc
      241 aggtcgacca gtccctgatg ctggcgatcg acgctatcga tgtgggtcac gaagtgttcg
      301 ctccaccgga gaaaggtcat gctttggctt ccttgtgctg gctaccgttc accggcagcg
      361 tgatgcggaa agtggtgccg tgtccagggt ttgagcgtac atcaatccgg ccctggtgtt
      421 tttcgatgat gttgaaagag atcgacaatc ccaggccggt gccctggcca accggcttgg
      481 tggtgaagaa cggctcgaac agtcgcctct gcacctccgg tgacaagccc tgcccgctgt
      541 cctcgatctc gacccagacc gactgcccgt cgtggctggc ggttcggacg acgatgcgtc
      601 cgcgttgctc gatcgcctgg gctgcgttca ccagcaggtt catgaacacc tggttgagtt
      661 gcgagggcag gcagcggaca ttcggcagct cgccgtactg gcgctcgaca tcggccttgt
      721 acttgagctc gttccaggcg atgttgatcg tgctgtcgag gcaggcgtgc aggtcagccc
      781 attgccagtc attctcgccg ctgcgcgaaa agtccttgag gtcgagaatg atccggcgta
      841 cccgttcgag cccttccttg ctttcgcgga tcagcttcgg caggtcgtcg atgatgtact
      901 cgtagtcgca ggccaccttg aggcggcgcg catcttccag ggcggcaacg ctgtgcggcg
      961 gtggcaattg ctcgacacgg acgtaggcgg cttcgatcgc cagcaggtca tcgacgtatt
     1021 tctggagcgt gccgagattg gaatggacga aaccgatcgg gttgttgagt tcgtgcgcga
     1081 caccggccgc gagctggccg atggccgaca tcttttccga ttgcagcagt tgcaggtgcg
     1141 cttgttcgag gcgttccttg agttgcgtga cttcggcgag gcggcgcgcc agtgacgctt
     1201 cggcacgcgc gcggcgtacg atgcgccaga ggtcgttgcc gagcaactgc acggtggtca
     1261 gatcgacgtc gttgtagtcg tcgggcttgt tgccgacgcc gatcatcatc cgcaccgcgc
     1321 tttcctcgat caccggtacc gagatcagcc gttgcagcgc cgcgtgcccg tcgggcaggc
     1381 catgtttgtc ggcgtagtcg gcgtagtggt tgaaagtgac cggctgcttg cggcggaagc
     1441 aatcggccca tatcccggcc tggctgatcg ggtagtggct gtcgaaggcg gctttgcagc
     1501 cctgcagcgc gccggcagtc caggtgacga gttcgatcgt ttcctggtcg tcgttgacaa
     1561 aatgcagaaa gccgatccgg ctgccggtga ggcgttccgc aagttcaaga ccgtgcgcca
     1621 ggaattccct ttccggcaga ctgccgccga cctcgttgat ttccagcaac gcccgggtac
     1681 gcgtttcgag ttggtggcgg cgggtaatgt cgagggcgat gccgccaagc agggcagggc
     1741 cgtcgtcctg cgggatgacg aaccgggtcg attcgtagat ggcgccaccg tag
//
LOCUS       DAOSIT010000520         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_56875_length_1792_cov_8.332182, whole genome shotgun sequence.
ACCESSION   DAOSIT010000520 DAOSIT010000000
VERSION     DAOSIT010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56875_length_1792_cov_8.332182"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLL14_10600"
     CDS             <1..450
                     /locus_tag="PLL14_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOG04421.1"
                     /translation="LVKGASGILAFDAVEAAVPEVGPCIRCGSCTRACPMGLLPLEMA
                     AHIRSGDLDGAVGYSLSDCISCGCCAYVCPSHIPLVQYFSHAKGELSARERAKLRSEA
                     GKRLAEARLARLERDAREKAEVAARRKAERAAVKARAPAPQPLEAST"
     gene            447..1520
                     /locus_tag="PLL14_10605"
     CDS             447..1520
                     /locus_tag="PLL14_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG04422.1"
                     /translation="MTRPVEMPVAAPYAATPNSVSRVMIIVMVALTPATLFGFWLYGW
                     PAIYLWMITVGAAAGGEAVCLQLQGRAVAPVLLDGSAVLTGWLLALSLPPWAPWWIGV
                     FGGLFATVLGKQVFGGLGQNLFNPAMVARVALLISFPVHMTAWVAPLPIVAAAAPGPI
                     AGLLITLKGMQPAFDTVASASLLGFAKTELSRGVDLAQSLLHAPIQGWYGSRAGSLGE
                     TATWLIAAGGVAMIFLRTITWHIPLAMLLGIAVPAAILNAIDPARYMEASTHLLSGGA
                     MLGAFFIATDYVTSPNTRRGQLVFGAGCGVLTYVIRTWAGYPEGVAFAVLLMNSLTPV
                     IDSYIRPRIYGRNRQGVALEPGR"
     gene            1530..>1792
                     /locus_tag="PLL14_10610"
     CDS             1530..>1792
                     /locus_tag="PLL14_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOG04423.1"
                     /translation="MQIGVAYSEAGQQVWLTIEVPDTSSVCDVIKHSGILKSFPTIDL
                     ETQKVGVFGRLVKLDAPLRPGDRVEIYRPITCDPQTVPRRDGS"
BASE COUNT          261 a          600 c          597 g          334 t
ORIGIN      
        1 ctggtcaagg gggcgagtgg catcctcgcc ttcgatgccg tcgaggcggc ggtgcccgag
       61 gtcgggccct gtatccgctg cggttcctgc acgcgcgcct gtccgatggg cctgctgccg
      121 ctggaaatgg cggcccacat ccggagcggc gacctcgacg gggccgttgg atactcgctc
      181 agcgattgca tttcgtgcgg ctgctgcgcc tatgtctgtc cctcgcacat tccgctggtg
      241 cagtacttca gccacgccaa gggcgagttg tcggcgcgcg agcgtgccaa gctgcgcagc
      301 gaggccggca agcgcctggc cgaagcgcgc ctcgcccgac tcgaacgcga tgcccgcgaa
      361 aaagccgagg tggccgcacg ccgcaaggcc gagcgtgccg ccgtgaaggc gcgtgcgccc
      421 gctccccagc ccctggaggc aagcacatga cgcggcctgt ggaaatgccg gtggccgccc
      481 cctatgccgc caccccgaac agcgtcagcc gggtcatgat catcgtgatg gtcgcgctga
      541 cgccggcgac cctgttcgga ttctggcttt acggctggcc ggcgatctac ctctggatga
      601 tcaccgtcgg cgccgccgcc ggtggtgaag cggtgtgcct gcaactacag ggacgtgccg
      661 tggcaccggt cctcttggat ggatcggcgg tgctgaccgg ctggctcctg gcgttgtcgc
      721 tgccgccctg ggcaccgtgg tggatcggcg tcttcggtgg gcttttcgcg acggtgttgg
      781 gcaagcaggt tttcggcggt cttggccaga atctgttcaa tccggccatg gtcgcgcggg
      841 tggcgctgct gatttccttc ccggtgcaca tgaccgcctg ggtggcgccg ctgccgatcg
      901 tcgcggcggc cgcgccgggg cccattgccg ggctcctgat caccctcaag ggcatgcagc
      961 cggccttcga caccgttgcc agcgcgtcgc tgctcggctt tgccaagacc gaactgtcgc
     1021 gcggcgtcga cctggcgcag tcgctgctgc atgcgccgat ccagggctgg tacggcagcc
     1081 gcgccggcag tcttggcgaa acagccacct ggttgattgc cgccggcggc gtggcgatga
     1141 tcttcctgcg caccatcacc tggcacatcc cgctggcgat gttgctcggc attgccgtac
     1201 cggcggcgat tctcaatgcc atcgacccgg cacggtacat ggaagcgtcc acccatctgc
     1261 tctccggcgg tgccatgctc ggggctttct tcattgctac cgactatgtc acctcgccca
     1321 atacgcgacg cgggcaactg gtcttcggcg ccggctgcgg cgtgctgacc tacgtgatcc
     1381 gcacctgggc gggttacccg gaaggcgtgg cctttgccgt gctgctgatg aactctctga
     1441 ccccggtcat cgacagttac atcaggccgc ggatctatgg acgcaatcgc cagggcgttg
     1501 cgcttgaacc cggccgttag gagaaaaaca tgcagatcgg agtggcctac tcggaagccg
     1561 gccagcaagt gtggctcacc atcgaagtcc cggatacgtc cagcgtgtgc gacgtgatca
     1621 agcactccgg tattctcaag tccttcccga ccatcgatct ggaaacccag aaggtggggg
     1681 tcttcggccg gttggtgaag ctcgatgcgc ctctccggcc gggcgaccgg gtagagatct
     1741 accgtccgat cacctgcgac ccgcaaaccg tgccgcgccg ggacggtagc ga
//
LOCUS       DAOSIT010000521         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57050_length_1788_cov_9.366994, whole genome shotgun sequence.
ACCESSION   DAOSIT010000521 DAOSIT010000000
VERSION     DAOSIT010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57050_length_1788_cov_9.366994"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLL14_10615"
     CDS             complement(<1..415)
                     /locus_tag="PLL14_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG04424.1"
                     /translation="MPPLPVITSTKLSLIDKKLSGKVRVMALPETTADLLRKLSKELT
                     RMRALLIDRYPNARNSLRIMLSSMGITAVHNAGSSAEVLRQVRAHNFDIILSDYQLED
                     GRDGQQLLEELRQQHLVPLSTVFFIITSERGYHNVV"
     gene            complement(565..>1788)
                     /gene="ahpF"
                     /locus_tag="PLL14_10620"
     CDS             complement(565..>1788)
                     /gene="ahpF"
                     /locus_tag="PLL14_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOG04425.1"
                     /translation="AIPGRFHFETFISLSCHNCPDVVQALNLMAVLNPGISHEMIDGA
                     LYQDEVTARQIMGVPTVLLNGKHFGQGRMTLEEIIGQIDTGALQREADKVSARAAFDV
                     LIVGGGPAGAAAAIYAARKGIRTGVVSERFGGQVLDTLAIENFISVKETDGPKLAAAL
                     EQHVREYDVDIINLQRAEALLPGTDGGLIELRLASGATVRSKTVILATGARWREMNVP
                     GEKEYRGHGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGVVSHVTLLEFESSL
                     RADAILQKKLFSLANVTVITQALSTEVSGDGKKVNGLVYTDRKTGQVHRIDLEGIFVQ
                     IGLLPNSEWLKGTIALSARGEIEIDARGETSLPGVFAAGDVTTVPYKQIVIAMGEGSK
                     AALSAFDYLIRRSVA"
BASE COUNT          315 a          553 c          551 g          369 t
ORIGIN      
        1 aaaccacgtt gtggtagccg cgttcgctgg tgatgatgaa aaagacggta ctcaagggca
       61 ccagatgctg ctgacgcaac tcctccagca actgctggcc gtcacgtccg tcctcgagct
      121 ggtaatcgga aaggatgatg tcaaagttgt gcgcccggac ctgtcggagc acctccgctg
      181 aactgccggc gttatgcacg gcggtgatac ccatcgagga cagcatgatc cgcaacgaat
      241 tgcgggcatt ggggtagcga tcaatcagca atgcccgcat gcgggtcagt tctttgctta
      301 gcttgcgcag caggtcggca gtggtctccg gtagtgccat gacgcgaacc ttacccgaca
      361 gtttcttgtc gatcaagctc agtttagtgc ttgtgatgac cggcagtggc ggcacttgcg
      421 gacaccgccc gcccgccgct cctctgggtt gcgctgcttc tgaaccggac ctgcgctttc
      481 aagccggaat gggcgtgcct ccgcgaactg aaataaagcc tgccggtggg tcggcggtca
      541 tcctgtggtc gtgctggtga gcgctcacgc aacggaacgg cgtatcaggt aatcaaaggc
      601 gctgagcgct gccttggagc cttcacccat ggcgatgacg atctgcttgt agggcaccgt
      661 cgtcacgtcg cctgccgcga aaacgccagg cagcgaggtc tccccacggg cgtcgatttc
      721 gatctcgccg cgcgcggaga gggcgatcgt tcccttgagc cattcgctgt tgggcagcag
      781 gccgatctgg acaaagatcc cttcgaggtc tatgcgatgc acctggccgg ttttccggtc
      841 ggtgtagacc aggccgttga ccttcttgcc gtcgccgctc acttcggtac tcagcgcctg
      901 ggtaatcacg gtcacattgg ccaggctgaa aagcttcttc tgcaggatcg cgtcggcacg
      961 caggctactc tcgaattcga gcagggtgac atgcgagacg acgcccgcga ggtcgattgc
     1021 cgcttcgacc ccggagttgc cgccgccaat gacagccacc cgcttgccct tgaacagcgg
     1081 tccatcgcag tgcgggcagt aggcgacgcc gtgcccccgg tattctttct cgccggggac
     1141 gttcatttcc cgccagcgtg ccccagtggc caggatcacc gtcttgctcc tgacggtcgc
     1201 gccgctggcc agccggagtt cgatcagtcc gccgtctgtg cccggcagca gggcttctgc
     1261 gcgctgcaga ttgatgatat ccacgtcgta ttccctgacg tgttgctcca gcgcagctgc
     1321 gagtttcgga ccgtcggtct ccttgaccga aatgaagttt tcgatggcca gcgtgtcgag
     1381 cacctgtcca ccgaatcgct cgctgaccac tccggtgcga atccctttgc gggcagcata
     1441 gatagcggcg gcggcgccgg ccggaccccc gccgacgatc agcacgtcga aggccgcgcg
     1501 ggccgagacc ttgtccgcct ctcgttgcag ggcaccggta tcgatctggc caatgatttc
     1561 ctcgagcgtc atgcgacctt gcccgaaatg tttgccgttc agcaggacgg tcggtacccc
     1621 catgatctgg cgagccgtca cctcatcctg gtacagcgca ccatcgatca tttcatggct
     1681 gatgccgggg ttcagcacgg ccatcaggtt gagtgcctgc acgacgtcag gacagttgtg
     1741 gcaggaaagc gagatgaagg tttcgaagtg gaagcgaccg ggtatggc
//
LOCUS       DAOSIT010000522         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57218_length_1784_cov_9.532099, whole genome shotgun sequence.
ACCESSION   DAOSIT010000522 DAOSIT010000000
VERSION     DAOSIT010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57218_length_1784_cov_9.532099"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1628
                     /locus_tag="PLL14_10625"
     CDS             57..1628
                     /locus_tag="PLL14_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOG04426.1"
                     /translation="MSLHAALRTGVKSVFLGCEEAADALFRGEDNPLRHLGAMGLYLL
                     WIVIGSGLYLYIVLDTGIDAVYRSIGNLALQQRYFGGILRSLHRYASDGFMLVMLLHL
                     LREWCYGRYFGFRWYSWVTGVPLLWLAYLAGIGGYWIVWDQLAQLSAIATTELLDWLP
                     IFSEPSARNFLTPDSLSDRFFTMLVFIHLGVPLLLILGLWAHVHRIGHVDYLPSRRVM
                     LATLAALIALSLFAPALSNPPANLATTPGELGMDWFILFVHPLTDLTSPAIVWSLLFS
                     LTALLFALPLLPHPRPQPAAVVDAANCTGCDRCLADCPYAAITLQAHPQRPGLKLAVV
                     DPDLCASCGICAGSCPSSTPFRRQEMLVTGIDMPQQPVNALREQLEQALARLAGRTRI
                     VVFSCARGADAGFLGATDTAVIPLLCAGMLPPAFVEYALRGGADGVLLTSCRSGGCDF
                     RLGERWTGDRLQGRREPHLRRTVPAARVLLLATTVHDRSALADSLARFRSRLESEADA
                     SDRLPPYHRKASRHA"
BASE COUNT          263 a          620 c          533 g          368 t
ORIGIN      
        1 tcaatatcat caacagctaa tcattgtggc cggtaccggc ttgggaatcc agcgacttga
       61 gtttgcatgc ggcattgcgt acgggtgtta aaagcgtctt cctgggctgt gaagaggctg
      121 cagatgccct tttccgtggc gaggacaatc cgttgcgtca cctcggcgcg atgggactgt
      181 atctcctgtg gatcgtcatc ggcagcgggc tctatctgta catcgtgctc gacaccggca
      241 tcgacgccgt gtaccgctcg attggcaatc tcgctctcca gcagcggtat ttcggcggca
      301 tcctgcgcag cctgcaccgc tatgcgtccg acggcttcat gctggtgatg ttgctgcacc
      361 tgctgcgcga atggtgctac ggtcgctatt tcgggttccg ctggtattcg tgggtgaccg
      421 gcgtgccgct gctctggctg gcgtaccttg cgggcatcgg cggttactgg atcgtctggg
      481 accagctcgc gcagttgtcg gccatcgcga cgaccgagtt actcgactgg ctgccgatat
      541 tcagcgaacc atcggcgcgc aacttcctga ctccggactc gctcagcgac cgcttcttca
      601 ccatgctggt gttcatccac ctgggcgtgc cgctgctgct gattctcggc ctctgggcgc
      661 acgtccatcg catcggccat gtcgactacc tgccttcccg gcgggtcatg ctggccacgc
      721 tggcagcgct gatcgcgctc tcgctattcg ccccggcgct gagcaacccg ccggccaacc
      781 tggccacgac gcccggggaa ctcggcatgg actggttcat cctgttcgtc cacccgctga
      841 ccgacctcac ttcgccggcg atcgtctggt cgctgctctt cagcctgacc gccctgctct
      901 tcgccctgcc gctgctgccg cacccgcggc cgcagccggc agccgtcgtc gatgcggcca
      961 attgtaccgg ttgcgaccgt tgccttgccg actgccccta tgccgcgatc accctgcagg
     1021 cgcacccgca gcggcctggt ctgaagcttg ccgtggtcga tcccgacctt tgcgcgagtt
     1081 gcggaatctg cgccgggtcc tgcccgtctt cgacgccgtt ccgaagacag gaaatgctgg
     1141 tcaccggcat cgacatgcca cagcaaccgg tcaacgcgct gcgcgaacaa ctcgaacaag
     1201 ccctggcgcg cctcgctggc cgcacgcgca tcgtcgtctt cagttgcgcg cgcggtgccg
     1261 acgcaggctt cctgggcgcc accgacacgg cggtgattcc cctgctgtgc gctggcatgt
     1321 tgccgccggc gttcgtcgaa tacgcgctgc gcggtggtgc cgacggggtt ctcctgacct
     1381 cctgccggag cggcggttgc gatttccgcc tcggcgaacg gtggaccggg gatcgcctgc
     1441 aaggccggcg cgaaccccat ctccgtcgaa cggtaccggc cgcacgcgta ctcctgctgg
     1501 ccaccacggt gcacgatcgc agcgcgctcg ccgacagtct ggcccgtttc agaagccggc
     1561 tcgaaagcga ggcggatgcc agcgacagac ttccacccta tcaccggaaa gcctcacgtc
     1621 atgcttaaac ccaccgccat cgtcggtcag gtgatcctct acggcgcttt cgccgctttc
     1681 atcggctact ttgcgacttc gcccaagtac cggcaggttg ccgacgacat cgcactgatc
     1741 aagctgtcga tcagccacct cggcgatcgc gaatgtcgca agcg
//
LOCUS       DAOSIT010000523         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57485_length_1779_cov_3.943155, whole genome shotgun sequence.
ACCESSION   DAOSIT010000523 DAOSIT010000000
VERSION     DAOSIT010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57485_length_1779_cov_3.943155"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="PLL14_10630"
     CDS             complement(<1..1717)
                     /locus_tag="PLL14_10630"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOG04427.1"
                     /translation="MTFPDPLAIPHISFRYDGKDANELLPTWQTAAATATNARGTLEQ
                     RTFRDHGTGLSITIHTQRFTDFPAIEWVAEFENAGAADTPLIENIQAMDIMVPIPPQE
                     RLRLHHANGSLCRMDDFLPQTTELPPHTQKYLAPIGGRSSNGVLPFMNLQRSGAGMVI
                     GIGWSGQWCASWHRDHERLRMTAGQQQTRLRLQPGEKIRTPRILILPWEGNDPELGNN
                     LLRQLLLAHYVPRIGDTLVMPPVAQCLQAYFYLTGQASERFEMKALPKAAAAGVEVYW
                     IDACWYGNQANWWEAVGSWDVNRERFPNGVRPIRDAAHAAGMKFILWVEPERVRHHSL
                     IHRAHPEFLLASEHDTTNFLFDLGNPDARRYLTELLSDIIAENGLDIYRQDFNTDPLP
                     YWQAADAPDRSGMTENRYVQGLYTLWDELRQRHPGLWIDNCASGGRRIDLETLSRSLP
                     LWPSDFFDTCGLPSGLGLHVGDQCINAGLARWVPLFGGGVWNFTPYGTRSEIIGGFTF
                     GFHIDHANFPDDDVATNLTGNDVLARGVTLLDDEFPLPAAQAAIREWRSVRPFFTGDF
                     HLLLPL"
BASE COUNT          354 a          499 c          572 g          354 t
ORIGIN      
        1 tgagcggcag caggaggtgg aaatcgccgg tgaaaaacgg gcgcacactg cgccactcgc
       61 gaatcgcggc ttgtgcggcc ggcagcggaa attcatcgtc caacagcgtc acgccgcgcg
      121 ccagaacatc attgccggtc aggttggtgg cgacgtcatc atcgggaaag ttggcgtgat
      181 cgatatggaa cccaaaggtg aagccgccga tgatttcact gcgagtgcca tagggtgtga
      241 agttccacac gccgccgccg aagagtggga cccagcgtgc cagaccggca ttgatgcatt
      301 gatcgccgac atgcaggccc aatccggacg gcagaccgca ggtgtcgaaa aaatcgctgg
      361 gccagagcgg caaggaacgc gaaagcgtct cgagatcaat acgccggccg ccgctggcac
      421 agttgtcaat ccacagtccc ggatgccgct ggcgcagttc gtcccagagc gtgtagagac
      481 cttggacata acggttttcg gtcatgccgc ttcgatcggg tgcgtcggca gcctgccaat
      541 acggcagggg atcggtgttg aagtcctgtc ggtagatatc cagaccgttt tcggcaatga
      601 tatcgctgag cagttcggtg agataccgcc gcgcgtcggg attgccgaga tcgaacaaaa
      661 aatttgtcgt gtcgtgctcg ctggcgagca gaaactccgg atgcgctcga tgtatcagcg
      721 agtggtggcg cacgcgttcg ggctcgaccc aaaggataaa cttcataccg gctgcatggg
      781 cggcgtcgcg aatgggccgt acgccatttg gaaagcgttc gcgattgaca tcccacgagc
      841 cgacggcttc ccaccagttg gcctggttgc cataccagca ggcgtcaatc caatacacct
      901 cgacaccggc cgcggcagcc ttcggcagcg ccttcatttc gaagcgctcg gacgcttggc
      961 cggtcaggta aaaatacgct tggagacatt gcgcgaccgg cggcatcacg agcgtgtcgc
     1021 cgatgcgggg cacgtaatgc gccagcagaa gttggcgcag gagattgttg ccgagctctg
     1081 ggtcgttgcc ttcccatggc aggatcagaa tgcgcggcgt gcggattttt tcgccgggct
     1141 gcaagcgcag gcgggtctgc tgctgtccgg cggtcatgcg caatctctcg tgatcacgat
     1201 gccagcttgc gcaccattgg ccggaccagc cgatgccaat aaccatgccg gcgccggacc
     1261 gctgcagatt catgaatggc aataccccgt tcgaggagcg cccgccgatc ggcgcgagat
     1321 atttttgggt gtgcggcggc agctccgtgg tttgtggcag aaaatcatcc atgcgacaaa
     1381 gactgccgtt ggcgtggtgc aatcgaagcc gttcttgcgg cggtattgga accatgatgt
     1441 ccatggcctg aatgttctcg atgagaggag tatcggccgc gcctgcattc tcaaactccg
     1501 ccacccattc gatcgctggg aaatcggtga acctctgcgt gtgaatggta atgctcaggc
     1561 ctgtgccgtg atcgcgaaat gttcgctgct cgagcgtgcc gcgggcattg gtcgcagtcg
     1621 ccgccgccgt ttgccatgtg ggaagtagct cgttcgcgtc cttgccgtca taacggaacg
     1681 agatgtgcgg aatggcgagg gggtccggaa atgtcatggt gcatgccttc gtcagagtta
     1741 tcatgccatg tgcggcggtt cacctgcatc cattggggg
//
LOCUS       DAOSIT010000524         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57573_length_1777_cov_10.903600, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000524 DAOSIT010000000
VERSION     DAOSIT010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57573_length_1777_cov_10.903600"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLL14_10635"
     CDS             <1..479
                     /locus_tag="PLL14_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOG04428.1"
                     /translation="YPFLDNEQVVIAPLGHWAAERSIELQALSGERFILRETGSGSRR
                     TIDTHLAQIGVKLEIKLAVNSNEAIRDLVASGMGLAVLSRHALPMNPASEGLCILDVQ
                     GFPLRKPWLVIHLKSKILSLPARVFLEELLLRVSARGQSHTGEQAALGADSSSSIP"
     gene            complement(485..>1777)
                     /locus_tag="PLL14_10640"
     CDS             complement(485..>1777)
                     /locus_tag="PLL14_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOG04429.1"
                     /translation="KQFFAQLYEHLDNGAFALALATCERLLAQDSGFLLDALRLKGEI
                     LNTLGRHAEAQNVYQQVLAQRVVPWARMGLAFALRGQQRLAEAESLGQSLVDEFPEFT
                     AGYDFLAEVREEMGKPEEAQEVLLRAAVISPKNSSRQRMVGDVAARNNDLPTAEKAYG
                     KVLERRRGSSLKNIDDYTNLSRVMVDRGHTAAARQITDELRRDWRGSRQGEFAALVME
                     SLCANKEGDPAKARQAVEKALLLNDALKDEGKLSGVSQKLTLDLAQACLATGDEASAR
                     NILREVAAENHEDRHMIANVQGIYARAGKEKDGQALLAEVGKEIVELNNRGVLAARRG
                     DVEGSVKLLMEAAERVPNLQFLINATKAIFTLLDIKGWDPTLARRGLRYLQMAQAKDL
                     RNARVISTRELYQRVARKYGVAIVSLANAHRQGEEPGG"
BASE COUNT          299 a          569 c          534 g          375 t
ORIGIN      
        1 gctacccgtt tctcgacaac gagcaggtgg tgatcgctcc gctcggtcat tgggccgccg
       61 agcgctccat cgaactgcag gcgctgagcg gcgagcgctt catccttcgc gaaacgggtt
      121 cgggttcgcg ccggacgatc gatacgcatc tggcgcagat cggagtgaag ctggagatca
      181 agctggccgt caacagcaac gaagcaatcc gcgatctggt tgcaagtggc atgggattgg
      241 cagtgctttc ccgacacgca ctgccgatga atcctgcgag cgaaggcctg tgcatcctcg
      301 acgtgcaggg attcccgttg cgcaagccgt ggctggtgat ccacctgaaa tcgaagatcc
      361 tgtcgttacc ggccagggtg ttcctcgaag aactgctgct ccgggtgtcc gcccgtgggc
      421 aaagccatac tggtgagcaa gctgcgttgg gggcggactc ctcgtcgtcc atcccctgaa
      481 ctccctatcc ccccggctcc tcaccctggc ggtgagcgtt ggccagcgag acaatggcca
      541 ccccgtactt gcgtgcgacc cgctgatata gctcgcgtgt cgagatcacg cgggcgttgc
      601 gcaggtcctt ggcttgcgcc atctgcaggt agcgcaggcc acgtctggcc agtgtcggat
      661 cccagccctt gatgtccagc agcgtaaaaa tggctttggt ggcgttgatc aggaattgca
      721 ggttgggtac ccgttctgcc gcttccatca gcagcttgac cgaaccttcg acgtcgccgc
      781 ggcgagcggc caggacgccg cgattgttga gttccacgat ttccttgccg acctccgcaa
      841 gcagcgcctg gccgtccttt tccttgcccg ctctggcgta gattccctgc acgttggcga
      901 tcatgtggcg gtcttcgtga ttttcggcgg ccacctcgcg aaggatattc cgcgcactcg
      961 cctcgtcgcc ggtggccaga cacgcctgcg ccaggtcaag ggtcagcttc tgcgagactc
     1021 cggacagctt tccttcgtct ttcaaggcat cgttcagcag cagtgctttc tcgacggcct
     1081 gcctggcctt ggcgggatcg ccttccttgt tggcgcagag gctctccatg accagggcgg
     1141 cgaattcccc ttgccggctg ccacgccagt cccttcgcaa ttcatcggta atctgtcggg
     1201 cagcggccgt atggccgcgg tcaaccatca ctcgcgagag attggtgtag tcgtcgatat
     1261 tcttcagtga tgagccgcgg cgacgctcca gaaccttgcc gtaggctttt tcggcggtcg
     1321 gcaaatcgtt gttgcgtgcc gcgacatcgc cgaccatccg ttgtcgtgac gagttcttcg
     1381 gagaaatcac cgccgcacgc agcagcactt cctgggcctc ttcgggctta cccatttcct
     1441 ctcgtacttc ggcgagaaaa tcgtagcccg cggtgaattc cggaaactcg tcaaccagcg
     1501 actggcccag ggactcggcc tcggccagtc gctgttgccc ccgcaatgca aaggccagac
     1561 ccatgcgtgc ccatggcacc acccgctgcg ccagcacctg ctgatagacg ttctgggcct
     1621 cggcatgtcg ccccagggtg ttgagaatct cgcctttgag acgcagcgcg tccaggagaa
     1681 agccgctgtc ctgcgccagc aggcgttcgc aggtggctag cgccagggca aaagcgccat
     1741 tgtcgagatg ctcgtagagc tgcgcaaaga actgctt
//
LOCUS       DAOSIT010000525         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57626_length_1776_cov_9.476467, whole genome shotgun sequence.
ACCESSION   DAOSIT010000525 DAOSIT010000000
VERSION     DAOSIT010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57626_length_1776_cov_9.476467"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLL14_10645"
     CDS             complement(<1..637)
                     /locus_tag="PLL14_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04430.1"
                     /translation="MYRKLLNVVLFLMACMAFGAVAASDPPAAIESGIVKARVTAVAS
                     NYTSFTVRIGDGEEKLVLDEVAAKALQAKLRINPGDRIAVILGAPEAAGGTTVKEFKG
                     LEKQVGVGYRLLFLFIGLVVILACFAVFSTGNLRGFVIGADNRYSNSKIQIVLWFGSL
                     FVIYLGTLMLRVYVYGWDLLGGVGITENLLALSGLSAFTYGSAKAITSSQHE"
     gene            complement(727..>1776)
                     /gene="phoR"
                     /locus_tag="PLL14_10650"
     CDS             complement(727..>1776)
                     /gene="phoR"
                     /locus_tag="PLL14_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HOG04431.1"
                     /translation="NAMDAVPDGLVILDAHHQIQWSNRAAAGHLGIHLSRDHGAIIEQ
                     LVRTPGFAEYLDDPHAQAPLLLQSTAVRTRVYALRVVPLDEHNKLLVSLDVTDARRVE
                     AMRSTFVANVSHELRTPLTVVSGFLEHFADDGAMSPEQRRYFTRLMSDQTARMLSLVD
                     DLLMLSRLESEDAPATEEDVDMADLLAQLHSEAQSLSEGRHRIEVICEGPGLRGNRKE
                     YHSAFGNLVSNAIRYTPAGGEIGIEWLVRDGAGIFQVRDSGVGIAAEHLPRLTERFYR
                     VDRGRSRDTGGTGLGLAIVKHILLRHQAALRIESREGQGSTFRVEFPAWRLRPEGSDG
                     PRHGGEAIGMQDETG"
BASE COUNT          355 a          572 c          512 g          337 t
ORIGIN      
        1 tttcgtgctg gctggaggtt atcgctttcg ccgagccata ggtaaacgcg ctcaggccgc
       61 tgagcgccag cagattttcg gtaatcccga cgcctccaag caaatcccag ccataaacgt
      121 agacccgcag catcagcgtt cccagataga tcacaaacag gctcccgaac cacaacacga
      181 tctgaatctt cgagttgctg tagcgattgt ccgccccgat cacgaaacca cgcagattgc
      241 ctgtcgagaa cacagcgaag caagccagta tcaccaccag accgatgaac aggaagagca
      301 accgataacc cacgccaacc tgcttttcga gccccttgaa ctccttgacg gtagtgcccc
      361 ccgctgcctc cggtgctccc aggatcaccg ctatgcggtc gcccgggttg atgcggagct
      421 tggcctgcaa tgctttcgct gccacttcgt caagtaccag cttctcttcg ccatcaccga
      481 ttctcaccgt gaaactggtg tagttgctgg ccactgccgt gacccgggcc ttgacaattc
      541 cggactcgat cgcggctggg ggatcactcg ccgcgacggc gccaaaggcc atgcaggcca
      601 tcagaaacaa aacgacattc agcaacttgc gatacatgac aggttcctcc agaaaagaaa
      661 agcctgtgtt cagggctatg gtgtacggcc cggaacactg gctatccacg ctggccgtga
      721 tgaatcctac cccgtttcgt cctgcatccc aatagcttcg ccgccatgcc ggggcccgtc
      781 gctcccttcc ggtcgcaggc gccaggccgg gaactcgacg cggaaggtac tgccctggcc
      841 ttccctggat tcgatgcgca aggccgcctg gtggcgcagc aggatgtgtt tgacgatggc
      901 caggccaagg cccgtgccac cggtatcacg cgaacgtccg cggtcgacgc ggtagaagcg
      961 ctcggtcaac cgcggcagat gctcggccgc gatgccgacg cccgagtcac gcacctggaa
     1021 aatgcctgct ccgtcacgga ccagccactc gatgccaatc tcgccgcctg ctggcgtgta
     1081 gcggatggcg ttcgaaacga ggttgccgaa ggcgctgtgg tattccttgc ggtttcctcg
     1141 cagtcccggc ccctcgcaga tgacttcgat acgatgcctg ccctcgctga ggctctgcgc
     1201 ttcggaatgc agttgcgcca gcaggtcggc catatcgacg tcctcctcag tcgctggcgc
     1261 gtcctcggat tcgaggcgcg acaacatcaa aagatcgtct accaggctta acatgcgtgc
     1321 ggtctggtcg ctcatgaggc gtgtaaagta ccggcgctgc tcgggactca ttgcgccatc
     1381 gtcggcaaag tgctcgagaa atccgctgac tacggtcaag ggtgtacgta gctcgtgcga
     1441 gacgttggca acgaaggtgc tgcgcatcgc ctcgacccgc cgggcatcgg tcacatcaag
     1501 gctgaccagc aatttgttgt gctcgtcgag aggcaccacc cgcagggcgt aaacacgcgt
     1561 ccgcacggcc gtcgactgca acagaagggg cgcctgcgcg tgcgggtcat cgaggtactc
     1621 ggcaaaaccc ggcgtacgga ccaactgttc gatgattgct ccgtgatcgc gcgacaggtg
     1681 gattcccagg tgtccggcgg cggcacgatt gctccactgg atctggtgat gagcatcgag
     1741 gatcaccagg ccgtcgggaa ccgcgtccat cgcatt
//
LOCUS       DAOSIT010000526         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57756_length_1773_cov_10.720023, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000526 DAOSIT010000000
VERSION     DAOSIT010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57756_length_1773_cov_10.720023"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLL14_10655"
     CDS             complement(<1..473)
                     /locus_tag="PLL14_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOG04432.1"
                     /translation="MQGAASLPQRAASPLPAPSDWTFELLDEYHAVIRSTARRFALDT
                     YPNQLEIITAEQMMDAYASVGMPVNYRHWSYGKEFIATEKNYRRGQMGLAYEIVINAN
                     PCISYLMEENTMAMQALVIAHAAYGHNSFFKGNYLFRMWTDAASIIDYLVYAKDYV"
     gene            complement(500..>1773)
                     /locus_tag="PLL14_10660"
     CDS             complement(500..>1773)
                     /locus_tag="PLL14_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HOG04433.1"
                     /translation="QQIIDRRLAGKNKSIGNRERFLRRHKAQIQEAVKRAVAGRGIRD
                     IAQGEDIHIPRRDISEPVFGHGPGGRREMVHPGNKDFVRGDRIDRPQGGGAGRGTGQA
                     GDAGEGEDDFVFHLSKEEFMQVFFDDLALPNLIRTTLADAPEYKTHRAGFSSDGTPNN
                     LHIVCSMRGAIGRRIALGSESRRELTRLEGLLVVLRAQPATPSRRHEIEALERDIEAL
                     RAHLLRIPYLDPIDLRYRSRVRTPVPTARAVMFCLMDVSGSMDEARKDLAKRFFILLY
                     LFLTRHYERIDLVFIRHHTQAQEVNEHDFFHATETGGTVVSSALVLMEEIIRARYPSG
                     EWNIYGAHASDGDNWHNDSTLCRKLLAERLLPLCRYFAYIQVVEQEQNLWQEYALLAE
                     SSEAFAMRKASTADQIYPVFRDLFSKEGASK"
BASE COUNT          306 a          558 c          559 g          350 t
ORIGIN      
        1 acgtagtcct tcgcgtaaac cagatagtcg atgatcgacg ccgcgtcggt ccacatccgg
       61 aacaggtagt tgcccttgaa gaagctgttg tgaccgtagg ccgcgtgcgc gatcaccagc
      121 gcctgcatgg ccatcgtgtt ttcctccatc aggtagctga tgcagggatt ggcgttgatg
      181 acgatttcgt aggccaggcc catctggccg cggcgatagt tcttttcggt ggcaatgaat
      241 tccttgccgt agctccagtg gcggtagttg accggcatgc cgaccgacgc ataggcgtcc
      301 atcatctgtt cggcggtgat gatttcgagc tggttcggat aggtgtcgag agcgaagcgc
      361 ctggcggtgc tgcggatcac cgcgtggtac tcgtcgagca gttcgaaggt ccagtcgctc
      421 ggcgccggca ggggcgacgc cgcccgctgc ggcagcgacg ctgccccctg catgccacgc
      481 ctggggttca aggagtcgct catttgctcg ccccttcctt gctgaacaag tcgcgaaaca
      541 ccgggtagat ctggtcggcg gtgctcgcct tgcgcatcgc gaaggcttcg gacgactccg
      601 ccagcagcgc atactcctgc cacagattct gctcctgctc gacgacctgg atataggcga
      661 agtaacggca aaggggcagc agtctctccg ccagcagttt gcggcaaagc gtgctgtcgt
      721 tatgccagtt gtcgccgtca ctggcgtgtg cgccgtagat gttccattca ccactcgggt
      781 agcgggcacg gatgatttct tccatcagca ccagcgcgct cgacaccacg gtaccgccgg
      841 tttccgtggc atggaagaaa tcatgctcgt tcacctcctg tgcctgcgtg tgatggcgga
      901 tgaagaccag gtcgatcctt tcataatgcc gggtcaggaa aagatagagc aggatgaaga
      961 agcgcttcgc caggtccttg cgcgcctcgt ccatcgaacc cgagacatcc atcagacaga
     1021 acatcacagc cctcgccgta ggcaccggcg tgcgcacgcg gctgcgatag cgcaggtcga
     1081 tcgggtcgag gtagggaatg cgcaggaggt gtgcgcgcag cgcttcgata tcacgctcca
     1141 gagcttcgat ctcatgccgg cgtgacggcg tcgcgggctg cgcacgcagc acgacgagca
     1201 atccttcgag acgcgtcagt tcgcgccgcg attcgctgcc cagcgcgatg cgccggccga
     1261 tcgcgccgcg catcgagcac acgatgtgca ggttgttcgg cgtcccgtcg ctagaaaatc
     1321 cggcacgatg cgtcttgtac tcgggcgcat cggcaagcgt ggtgcggatc agattgggca
     1381 gcgccaggtc gtcgaagaag acctgcatga actcttcctt gctcaggtgg aagacgaaat
     1441 cgtcctcccc ctcgccggcg tcgccggcct gcccggtacc gcgcccagca ccaccgccct
     1501 gcgggcggtc gatgcgatca ccgcggacga aatccttatt gcccggatgc accatttcgc
     1561 gtcggccgcc cggcccgtgg ccaaacaccg gctcgctgat gtcgcggcgc gggatgtgga
     1621 tgtcctcgcc ctgggcgatg tcacggatgc cacgcccggc gaccgcgcgc ttcaccgctt
     1681 cctggatctg cgccttgtgg cggcgcagga agcgctcacg gttgccgatc gacttgttct
     1741 tgccggccag cctgcgatcg atgatctgtt gca
//
LOCUS       DAOSIT010000527         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57758_length_1773_cov_9.976717, whole genome shotgun sequence.
ACCESSION   DAOSIT010000527 DAOSIT010000000
VERSION     DAOSIT010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57758_length_1773_cov_9.976717"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLL14_10665"
     CDS             <1..684
                     /locus_tag="PLL14_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOG04434.1"
                     /translation="QGAMRIQRPNALELPYTREMMAGVLLRDPPWPRQALLIGLGAGS
                     LAKFIYHRLPATRITVVEIDPQVESVARLHFKLPDDPLRLRVCIADGADYMLQGDSLH
                     DYILVDGFDRNARAGVLDTLPFYQACRARLSDCGLLAVNLLGRERGFAASAERISKAF
                     EGRSLVFPSCDSGNTIAFATAGEPIDIPCVELINRARQCLKETGLNLLPTVARLQQQH
                     HLSDDRLRL"
     gene            884..>1773
                     /locus_tag="PLL14_10670"
     CDS             884..>1773
                     /locus_tag="PLL14_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOG04435.1"
                     /translation="MTQAHPSATVRDTPPDGSSPPLLPARWRTQLDAQLAGDERIMAW
                     LEIDLDARLHFSSGVLVVTSERLLSAEGKRWTSWTFRSGLSLSRRDHSGVGTLELTDA
                     DHLLAHWRYTLGADVAAGRLVDHFTRQLAYHVTGELPPPSTVALCPKCETPLLAGQEE
                     CPACSKEIHEPPSTWTLLRLGRFARPYRGRLLAAFALSLLTTAATLVAPYLTMPLMDK
                     VLIPYQNGQPIDPQLVAFYLSGLLGSSLFAWVLGWGRTYILALVSERIGADLRTTTYE
                     HLLKLSQEYFGGKRTGDLMAR"
BASE COUNT          302 a          604 c          540 g          327 t
ORIGIN      
        1 cagggtgcga tgcgcattca gcgccccaat gccctggaac tgccgtacac ccgcgaaatg
       61 atggctggcg tgttgctgcg tgacccaccc tggccgcgcc aggccttgtt gatcgggctc
      121 ggcgccggat cactggcaaa attcatctac caccggttgc cggcaaccag gatcaccgtc
      181 gtcgagatcg atccgcaggt cgagagcgtc gcccggctgc acttcaaact gcccgacgat
      241 ccgctgcggc tacgcgtgtg catcgccgac ggtgccgact acatgctcca gggcgacagc
      301 ctgcatgact acatattggt agacggtttc gatcgcaacg cccgcgcagg tgtgctcgac
      361 actctgcctt tctaccaggc ctgtcgtgcg cgcctgagcg actgcggact gctggcggtc
      421 aacctgctcg ggcgggaacg cggcttcgcg gcaagtgccg agagaatcag caaggctttc
      481 gaaggccgtt cgctggtctt tccttcgtgc gacagcggca ataccatcgc cttcgccacc
      541 gctggcgagc ccatcgacat cccctgcgtg gaattgatca accgcgccag gcagtgcctg
      601 aaagagaccg gactcaacct gttgccgacc gtcgcccggc tgcaacagca acaccatctg
      661 agcgacgacc gcctgcgcct ctgaacacga atcaggaggc cccggtcgtg tttgcccgtc
      721 cccggccgga tgcactctcg ccagcaaccc cgacagggca tcaggcatga cccgattgcg
      781 gctgtttcgc tggctgcggt cgcaacggcg ccgtcgcgca aggaccgcca tgccggggag
      841 atggcaggta tacttgcagc cttgttgcaa gaccggtcaa atcatgacgc aagctcatcc
      901 ctccgccacc gttcgcgaca cgccacctga cggatcgtcg ccacctttgc tgccggcccg
      961 ctggcggacc caactggatg cgcagctcgc tggcgacgaa cggattatgg cctggctcga
     1021 gatcgatctc gacgccaggc tgcatttttc atccggcgtg ctggtggtca ccagcgagcg
     1081 cctgctgtcg gccgaaggca agcgttggac cagctggact tttcgctcag gactttccct
     1141 cagccgccgc gatcattcgg gtgtcggaac gctggaactg acggatgccg accacctcct
     1201 ggcgcattgg cgctacacgc tcggtgccga tgttgccgcc ggccggctgg tcgaccactt
     1261 cacacggcaa cttgcctacc acgtcactgg agaactgccg cccccttcga cggtggcact
     1321 ctgcccgaag tgcgagacgc cgctgctggc cggtcaggag gagtgcccgg cctgcagcaa
     1381 ggagatccac gagccgccgt caacctggac gctgctgcgc ctggggcggt ttgccagacc
     1441 ctacagaggg cgcttgctgg ccgcctttgc gctgtccttg ctgaccacgg cggcaacgct
     1501 ggtggcaccg tacctgacga tgccgctgat ggacaaggtg ctgatcccct accagaacgg
     1561 ccagcctatc gacccgcaac tggtcgcgtt ctatctttcg ggcctgctcg ggtcgtcact
     1621 gttcgcctgg gtgctcggtt ggggacgcac ttacattctg gcgctggtat cggaacggat
     1681 cggcgccgac ctgcgtacca ccacctacga gcacctgctc aagctctcgc aggagtattt
     1741 tggcggcaag cgaaccggcg acctgatggc gcg
//
LOCUS       DAOSIT010000528         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57894_length_1770_cov_5.706122, whole genome shotgun sequence.
ACCESSION   DAOSIT010000528 DAOSIT010000000
VERSION     DAOSIT010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57894_length_1770_cov_5.706122"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLL14_10675"
     CDS             <1..403
                     /locus_tag="PLL14_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG04436.1"
                     /translation="AGLAAPQIGVGLRVVIFGMEGKPRYPDAEQVPWTVLINPVLTPL
                     SSVVDEGWEGCLSVPGLRGWVPRWRSLRYTGFDAGGRPIDRAVEGFHARVVQHECDHL
                     DGVLYPMRIRDFTRFGFVDELFPRGGAGVAE"
     gene            506..997
                     /locus_tag="PLL14_10680"
     CDS             506..997
                     /locus_tag="PLL14_10680"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOG04437.1"
                     /translation="MTPPYRILFVCMGNICRSPIAEAVTRSMAQRQGLATMLDLDSAG
                     THGHYHAGEAPDLRARRVAARRGYDLSRLRARLVIAADFDRFDRILAMDEANLAGLQR
                     LCPEDSRSKLGLFLDYATGLGISEIPDPYYGAESGFERVVDLCELAATGLLAEFARGR
                     LAR"
     gene            1025..>1770
                     /locus_tag="PLL14_10685"
     CDS             1025..>1770
                     /locus_tag="PLL14_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOG04438.1"
                     /translation="MSLFSRSTQQVLKHPAAFALQTLKSFSDNQGLLLAAAIAYYALL
                     SVVPLLILSVIALSHLVEQAELLATLGHYLEWLVPSQSKAFLNEVSAFLENRAAIGAV
                     LLLTMLFFSSLAFSVLEKAMAVIFAHRERVEKRHFLTSALLPYCFVLFLGVALLGVTV
                     TSIALQAIAQESLHFLGRDWSLGGFSGELLYLLGLSAEVFILTAIYLIMPVGRLRLRH
                     ALFGGVTATCIWEVIRHILIWFFTTLSKASV"
BASE COUNT          270 a          523 c          569 g          408 t
ORIGIN      
        1 tgccggactg gctgcaccgc agattggtgt cggcctgcgg gtagtgattt tcggaatgga
       61 gggcaaaccg cgttatccgg atgctgagca ggtcccatgg acggtgctga tcaatcccgt
      121 gctgacgccg ctgtcgtcag tggtcgacga gggctgggaa ggctgcctct cggtacccgg
      181 cttgcgtggc tgggtgccgc gctggcgcag cctgcgctac accggcttcg acgccggcgg
      241 ccggccgata gaccgtgccg tcgagggttt tcacgcgcgc gtggtacagc atgaatgcga
      301 ccacctcgac ggcgttcttt atccgatgcg tatcagggac ttcacgcgtt tcggttttgt
      361 cgacgaattg tttccccgcg gtggcgccgg tgtggccgag tagcccggag cgaggtcagc
      421 gacggcggca tgcgccgaca tggccgtcgc gggccgtggt gtggcggatc gggcgaatca
      481 ttgatctctg gcggggacaa cagccttgac accgccgtat cggattctct tcgtgtgcat
      541 gggtaacatc tgtcgttcgc cgatcgccga agcggtgacc cggagcatgg cgcagcgtca
      601 gggcctggcg acgatgctcg acctcgactc ggccggtacg cacggccatt accatgccgg
      661 tgaggcaccg gatctgcgcg cgcgacgtgt cgccgcccgg cggggatacg atctgtccag
      721 actgcgcgcc cgcttggtca ttgcggccga tttcgatcgt ttcgaccgta tcctcgcaat
      781 ggacgaggcc aatctggcgg gattgcagcg tctctgcccg gaggacagtc ggtcgaaact
      841 gggcctgttt ctcgattacg ccaccggact cggaatctcc gagattccgg atccctacta
      901 cggtgccgaa tcgggattcg aacgcgtcgt cgatctctgc gagttggcgg caacagggct
      961 gctcgccgag tttgcgcgtg gacggcttgc ccggtaacgg atcgccacga cccgaacggt
     1021 gagcatttcc ctcttcagtc ggtcaacgca acaggttctc aagcatccgg ccgcctttgc
     1081 cctgcaaaca ctgaagagtt tcagtgacaa tcaggggctg ctgctcgccg cggcgattgc
     1141 ctactacgcc ttgctgtcgg tcgttccttt gctgatcctc tcggtgattg cactctctca
     1201 cctggttgag caggcagaat tgctggctac ccttgggcac tacctggagt ggctggttcc
     1261 gagccagtcg aaagcatttc tcaatgaagt ttcggcgttt ctcgaaaacc gtgcggcgat
     1321 cggtgccgta ctgttgctga cgatgctgtt cttcagttcg ctcgctttct cggtactgga
     1381 gaaggcgatg gcggtgatct ttgcacaccg ggagcgcgtt gaaaaacggc acttcctgac
     1441 gtcggccctg ctgccctact gcttcgtgtt gttcctgggg gtcgccttgc tgggcgtgac
     1501 cgtcacgtcc attgcgctgc aggcgatcgc ccaggagagc ctgcatttcc tgggcaggga
     1561 ctggtctctt ggcggttttt ccggagaact gctctatctg cttggcctga gtgccgaagt
     1621 cttcattctc accgcgatct atctgattat gccggtcggg cgtttacggc tgcgtcacgc
     1681 cttgttcggg ggcgtcacgg caacctgcat ctgggaggtg attcgccaca tcctgatctg
     1741 gttctttacc acgctgtcga aggcgagtgt
//
LOCUS       DAOSIT010000529         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_57969_length_1768_cov_12.528313, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000529 DAOSIT010000000
VERSION     DAOSIT010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57969_length_1768_cov_12.528313"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..165)
                     /locus_tag="PLL14_10690"
     CDS             complement(1..165)
                     /locus_tag="PLL14_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04439.1"
                     /translation="MKSRVISVMATIATRHDQDRIYLPDMLSNFDCGSVMIKQWLKNK
                     DTVLFLGVWE"
     gene            358..1605
                     /locus_tag="PLL14_10695"
     CDS             358..1605
                     /locus_tag="PLL14_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HOG04440.1"
                     /translation="MNEKKLVTLDQLRGFIAATSEVEFQGCGQDDERYRHIEAVLKRF
                     GYGRLGRADKGLVLRYLERTTGYSRQQLTRLVAGYRRVGKLAKAYRAPKHGFVRRFTE
                     ADVALLAETDSLHNTLSGPATRYLMARALIIYGDTRYARLSTISVGHLYNLRQRRGYI
                     ERRRVFTKTRATGIPIGERRAPAPDGRPGFIRIDSVHQGDQDGIKGVYHINAVDCVTQ
                     WELIATCEKISETYLLPVIEALLAGFPFRILGFHADNGSEYINRKVAKLLDKLAAEFT
                     KSRPRHSNDNGLAETKNGAVIRKHLGYAHIPQRHAAEVNVWCAEHLNPYVNFHRPCLF
                     AETITDKKGKTRKRYPLRLIMTPFEKLASVSKVTEFLRPGITLDTLRATATAVSDNEA
                     AEQMNQARQRLFISINKRSKTAA"
BASE COUNT          392 a          551 c          487 g          338 t
ORIGIN      
        1 ctactcccat acgcccagga acagcacggt gtccttattt ttgagccatt gcttgatcat
       61 aacgctgccg cagtcgaagt tgctgagcat gtcgggcaag taaatgcgat cttggtcatg
      121 ccgggtggcg atggttgcca ttacggagat aacgcggctt ttcattcttg ccgctccctt
      181 cccctcacgg cggtgcgatt tccatttggt atcgcaagtg tactcgcggc gaggctcatc
      241 aggcgagcca tagtcttttc caacatggta tgagtctgag cggtgggtcg tgtttccaac
      301 gaggaatgag tctgagcgag ggtaaaagga tcgatcattc agggtatggt gatcgacatg
      361 aacgagaaga agctggtgac gctggaccag ttacgtggat ttatagcggc gacttcggag
      421 gtcgaatttc aaggatgcgg acaggacgac gaacgctacc gtcacatcga agcggtgctc
      481 aagcgatttg gctatggccg gcttgggcga gcggacaagg gactggtttt gcgctacctg
      541 gagcgcacga caggctactc ccggcagcaa ctgacgcgac tggtcgcagg gtaccggcgt
      601 gtcggcaagc tggccaaagc ctatcgcgcc ccgaagcacg gctttgtacg ccgctttacc
      661 gaagccgacg tcgccctgct ggccgaaacc gacagcctgc acaacaccct gtccggaccg
      721 gcaacacgtt acctcatggc tcgggcactg atcatctatg gcgatacccg ctatgcgcgg
      781 ctatccacga tctcggtcgg ccacctgtac aacctgcgtc agcgccgggg ctacatcgag
      841 cgcaggcgcg tattcaccaa gacccgagcc accggcatcc ccattgggga acgccgtgcc
      901 ccggcacccg acgggcgacc gggtttcatc cgcatcgaca gcgtccatca gggcgaccag
      961 gacggcatca agggcgtcta tcacatcaac gccgtcgatt gcgtcaccca gtgggaactg
     1021 atcgccacct gcgagaagat cagcgaaacc tacctgctgc cggtcatcga agccctgctg
     1081 gccggtttcc ccttccgcat tctgggcttt catgccgaca acggctcgga gtacatcaat
     1141 cgcaaggtgg ccaagttgct cgacaaactg gccgccgaat tcaccaaatc ccggccgcgc
     1201 cattccaacg acaatgggct ggccgagacc aagaacggcg cggtgatccg caagcatctg
     1261 ggctacgccc atatccctca gcgccatgcc gccgaggtca atgtctggtg cgccgaacac
     1321 ctgaatccgt acgtcaattt ccaccgtccc tgcctgttcg cggaaaccat cacggacaag
     1381 aaaggcaaga cacgcaagcg ctatcccttg cgtctgatca tgacgccttt cgagaaactg
     1441 gccagcgtgt caaaagtgac cgagttcttg cgcccgggca tcacgctcga caccttgcgt
     1501 gcaacggcaa ccgcggtgtc ggacaacgag gccgccgagc agatgaatca ggctaggcaa
     1561 cgacttttta tttccatcaa caaacgatcc aaaaccgccg cttgaactac ttcctcacaa
     1621 aatgcacccc aaacccggtc tgtggatttg tggacaattc gctggcaaaa agccgccagc
     1681 gaaccgggcc gcttgccgtg gataagtcgc aaagaaccgc gacttatcca ccgcgcgacc
     1741 ctttgcccac aagctccaca gaccacct
//
LOCUS       DAOSIT010000530         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_58861_length_1748_cov_7.377437, whole genome shotgun sequence.
ACCESSION   DAOSIT010000530 DAOSIT010000000
VERSION     DAOSIT010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58861_length_1748_cov_7.377437"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /gene="fliI"
                     /locus_tag="PLL14_10700"
     CDS             <1..1235
                     /gene="fliI"
                     /locus_tag="PLL14_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOG04441.1"
                     /translation="GGSAIEAEVVGFNGERLFLMPSEDVYGLSPGARVVALEAPSQPP
                     RLGQPPPARRRSIDRAKLVPVGESLLGRVLDGAGRPLDRLGPLGASSLRPLQSRPVNP
                     MSRAPIEQALDVGVRAINALLCVGRGQRMGLFAGSGVGKSVLLGMMARYTAADVIVVG
                     LIGERGREVKEFIEQILGEQGLRRAVVVAAPADVSPLMRLQGAAYATCIAEYFRDRGR
                     QVLLIMDSLTRYAMAQREIALAIGEPPVTRGYPPSVFARLPQLVERAGNGAEGGGSIT
                     GFYTVLAEGDDQQDPIADAARAILDGHIVLSRTLADAGHYPAIDIEQSISRAMVNLVS
                     PTHLEHIRKFKSLFSRYQRSRDLISVGAYAAGSDPQLDQAIAIYPRFERLLQQNLSQR
                     ADFASSVAQLLGLFGPGG"
     gene            1290..1736
                     /gene="fliJ"
                     /locus_tag="PLL14_10705"
     CDS             1290..1736
                     /gene="fliJ"
                     /locus_tag="PLL14_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOG04442.1"
                     /translation="MARPFSLQTVLELMQTRSDEATQRLARLIAVERDAKEKFAMLLQ
                     YRDEYTARFRQAVQNGLGQPEWRNYQEFLTRLDEAIKQQMQLVSVQETHTAAGQEDWK
                     RQRTKLKALDALSERHRASEARLEIRQEQKAQDEFAARSKDNEQES"
BASE COUNT          339 a          555 c          539 g          315 t
ORIGIN      
        1 ttggtggctc ggcgatcgaa gctgaagtgg taggcttcaa tggggaacga ctctttctga
       61 tgccctcgga agatgtctac ggtctgtcgc cgggagcccg ggtcgttgcc cttgaagcgc
      121 cgagccagcc gccgcgactc ggtcagccac caccggcacg tcgacgctcg atcgaccgtg
      181 caaaactcgt gccggtcggc gagtccttgc tcggccgagt gcttgatggc gccggacgac
      241 cgctcgacag gctgggccca ttaggcgcga gttctctccg cccgctgcag agtcgaccgg
      301 ttaacccgat gtcacgggca ccgatcgagc aggcgctcga cgtcggggtg cgtgccatca
      361 atgccttgct gtgtgtcggc cgcggacagc ggatggggct tttcgccgga tccggcgtcg
      421 gcaagagcgt attgctcggc atgatggccc gctataccgc agccgacgtc atcgtcgtcg
      481 gcttgatcgg tgaacgtggt cgtgaagtca aggagttcat cgaacagatt cttggtgaac
      541 aaggcctgcg gcgggcggtc gtggttgcgg cacctgccga cgtcagtccg ctgatgcgtc
      601 tgcaaggcgc ggcctacgcc acctgcattg ccgaatactt ccgggatcgg ggtcggcagg
      661 tcctgttgat catggattcg ctgacgcgct acgcgatggc gcaacgcgaa atcgctctgg
      721 cgatcggcga gccgccggtc acccgcggct acccgccatc ggttttcgcc cgtctcccgc
      781 aactcgtcga acgtgccggc aacggcgccg agggcggagg ttcgatcacc ggcttctaca
      841 cggtccttgc agaaggtgac gaccagcagg atcccatcgc cgacgcagcg cgcgccatcc
      901 tcgacggtca catcgtgcta tcacgcacgc tggccgacgc tggccactac ccggccatcg
      961 acatcgaaca atcgatttcc cgcgccatgg tgaatctggt gagcccaact catctggagc
     1021 acatcaggaa attcaagagc ctcttctcgc gctaccagcg ttcgcgcgat ctgatcagcg
     1081 tcggcgcata cgccgcgggt tctgatccgc aactcgacca ggccattgcc atctatcccc
     1141 gctttgagag attgctgcag cagaacctga gccaacgcgc cgacttcgcg agcagcgtcg
     1201 ctcaactgct cggcttgttt ggcccaggtg gctgacacct gttgcgctag acttccggca
     1261 cttcccttgt accagtcggc gagttgatca tggccagacc cttttccctg cagactgtcc
     1321 tcgaactgat gcaaacccgt agcgacgaag caacgcaacg cctcgccagg ctgattgcgg
     1381 tggaacgcga cgccaaggaa aagttcgcca tgctgctgca gtaccgcgac gagtacaccg
     1441 cgcgcttccg gcaagccgta cagaatgggc tggggcaacc cgaatggcgt aactatcagg
     1501 agttcctgac tcgtctcgac gaagcaatca agcaacagat gcagctcgtc agcgtgcagg
     1561 aaacgcacac ggctgccggg caggaggact ggaagcggca gcggaccaag ctgaaagcac
     1621 tcgatgcact ctccgagcgc catcgcgcta gtgaggcacg gctggaaata cgccaggaac
     1681 aaaaggcaca ggacgagttc gccgcgcgca gcaaggacaa tgagcaagag tcctgaacca
     1741 tgcatggc
//
LOCUS       DAOSIT010000531         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_59064_length_1744_cov_10.590882, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000531 DAOSIT010000000
VERSION     DAOSIT010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59064_length_1744_cov_10.590882"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="csx2"
                     /locus_tag="PLL14_10710"
     CDS             complement(<1..881)
                     /gene="csx2"
                     /locus_tag="PLL14_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOG04443.1"
                     /translation="MTINTLISFLGKGQANPQTGYRTATYRFDAGFSRRVAFFGMALT
                     EYLKPDRLILVGTAGSMWDVFFEREGEGADDDAMLQLMTAVEAGQVDDALLELPRREL
                     AQQLGMPVDCLLIPYARDPGEQAEVLQRLASVVQRDESLFLDVTHGFRHLPMLALVAA
                     RYLARVVGVKVEEVYYGALEMTPPDGETPVLRLAGLLAMLDWVDALATYDKDGDYGVF
                     APLLAADGMEKGRAELLRRAAYFERTGNPVRARETLGSVFPSVEAHRGPLGGLFSATL
                     RQRISWFRRGKRDDWELS"
     gene            complement(878..>1744)
                     /gene="csm6"
                     /locus_tag="PLL14_10715"
     CDS             complement(878..>1744)
                     /gene="csm6"
                     /locus_tag="PLL14_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HOG04444.1"
                     /translation="SPEDNRRAADGITALIRELTADPDCALHVSIAGGRKTMGFFLGY
                     ALSLYGRPQDKLSHVLVSAPFESSLGFYYPTPTSHVIEMPGGRLVDSASAQVTLAELP
                     FVSLRHGLPEALLTGVASYNETVEAARHALAPAELQIDLEARRVRAAGKAFALPPAEL
                     ALLSVFARRALRGEAPLPAPAKDLPDADWRQRYLVELRWIAGPMKDLDETERALRQGM
                     DGGYFSAHLSKLKKILRKELGPAAEPYLISDGGRRPRRYRLDLRPAAISYDTLEASPP
                     NGATTSNILPGA"
BASE COUNT          267 a          541 c          653 g          283 t
ORIGIN      
        1 gaaagctccc agtcgtcgcg cttgccgcgg cgaaaccagc tgatccgctg ccgtagcgtt
       61 gcgctgaaca ggccgccgag cgggccgcga tgggcctcga ccgaggggaa gacgctgccc
      121 agcgtttcgc gcgcacgcac cgggttgccg gtgcgttcaa agtaggcggc gcggcgcagc
      181 agttcggcgc gccctttttc catgccgtcg gcggcgagca gcggcgcgaa gacgccgtag
      241 tcgccgtcct tgtcgtaggt ggccagcgcg tcgacccagt cgagcatcgc cagcaggcca
      301 gcgagccgca gcaccggcgt ctcgccatcg ggcggcgtca tttcgagggc gccgtaatag
      361 acttcctcga ccttgacgcc aacgacgcgg gccagataac gcgcggccac cagtgccagc
      421 atcggcaggt ggcgaaatcc gtgcgtgacg tcgaggaaga gactctcgtc gcgctgcacg
      481 acgctggcca gtcgctgcag gacctcggcc tgctcgccgg ggtcgcgggc gtaggggatc
      541 agcaggcaat cgacgggcat gccgagttgc tgggcgagtt ctcggcgcgg caactccagc
      601 agggcgtcgt cgacctgtcc ggcctcgacg gcagtcatca gttgcagcat ggcgtcgtcg
      661 tcagcgcctt cgccttcgcg ctcgaaaaag acatcccaca tgctgccggc ggtgccgacc
      721 aggatcaggc gatcgggctt gaggtattcg gtcagagcca tgccgaagaa ggcgacacgg
      781 cgcgagaaac cggcgtcgaa gcggtaggtc gcggtgcgat agccagtctg cgggtttgct
      841 tggcccttgc cgagaaagct gatgagagtg ttgatggtca tgctccgggg agaatgttgc
      901 tggtggtggc gccatttggc ggtgacgcct caagggtatc gtaactgatc gccgccggcc
      961 gcaggtcgag ccggtagcgc cgaggccggc ggccgccgtc gctgatcagg tagggttcgg
     1021 cggccgggcc gagttccttc ctgaggatct tcttgagctt cgagaggtgc gccgagaagt
     1081 agccgccgtc catgccctgg cgcagcgcgc gctcggtttc gtcgaggtcc ttcatcggcc
     1141 cggcgatcca gcgcagttcg acgaggtagc gttgcctcca gtcggcgtcg gggaggtcct
     1201 tggccggtgc cggcaatggc gcttcgccgc gcagcgcgcg ccgggcgaag acgctgagca
     1261 gggcgagttc ggccggcggc agcgcgaagg ccttgccggc ggcacgcacg cggcgcgctt
     1321 cgaggtcgat ctgcagttcg gcgggggcga gcgcgtggcg ggcggcttcg acggtttcgt
     1381 tatagctcgc cactccggtg agcagcgctt ccggcaggcc gtggcgcagg ctgacgaagg
     1441 gcagttcggc gagcgtgacc tgcgcgctgg cgctgtcgac caggcggccg ccaggcatct
     1501 cgatcacgtg gctggtcggc gtcgggtagt agaagccgag gctcgattcg aagggtgctg
     1561 aaaccaggac gtgcgagagc ttgtcctgcg ggcggccgta gagcgacagc gcgtagccga
     1621 gaaagaagcc catcgtcttg cgaccgccgg cgatcgagac gtgcagcgcg cagtccgggt
     1681 cggcggtcaa ctcacggatc agggcggtga tgccgtcggc ggcgcggcgg ttgtcttccg
     1741 gcga
//
LOCUS       DAOSIT010000532         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_59158_length_1742_cov_8.730290, whole genome shotgun sequence.
ACCESSION   DAOSIT010000532 DAOSIT010000000
VERSION     DAOSIT010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59158_length_1742_cov_8.730290"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PLL14_10720"
     CDS             complement(<1..1481)
                     /locus_tag="PLL14_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOG04445.1"
                     /translation="MMKDRLSGQIDLAGLFGTGAAPLSIRTLALQGDDVARDAEAICA
                     VIGSPSFRDGSAAGISLPGGLATELLKAWHRSGVDIASQLHGSYALAIVDSARKCAFL
                     ATDRFAIETLCYRADGQVLAFADRADRVPGHGGELDPQAIFDYLYFHMIPAPRTIFQQ
                     VRRLPAAHSLLVDRNGAHVQRHWPLQFDEQHRQSFAPARDGFRELIRESVAEQITGHQ
                     RVGAFLSGGTDSSTVAGMLGQITGKPAPVYSIGFDAEGYDEMEYARLAATHFGCEHHE
                     YYVTPADLLDSIPAVAQHHDQPFGNSSALPAYYCARIAQADGCTRMLAGDGGDELFGG
                     NSRYAMQRLFEFYHHLPPGMRRAIEPLCEDGSPLRRIPGIKQATGYVRHARVPMPDRL
                     QSFNLIMQLDPASVLNPDFLAQLDLNEPSRHMQATWNECQAPSLVNRMLAYDWRYTLA
                     DSDLPKVRGATQMAGIEVAYPLLSDRLTDFSMALPPDWKLRRF"
BASE COUNT          309 a          506 c          594 g          333 t
ORIGIN      
        1 ttgaagcggc gcagtttcca gtcgggcggc agcgccatcg agaaatcggt cagcctgtcg
       61 gagagcagcg gataagcgac ttcgataccg gccatctgcg tggcgccgcg tactttcggc
      121 aggtcactgt cggcgagtgt gtaacgccag tcgtaagcga gcatgcgatt gaccagactc
      181 ggcgcctggc actcgttcca ggtggcctgc atgtgtcttg acggctcgtt caggtcgagt
      241 tgcgccagaa agtccgggtt gagcacgctg gccggatcga gttgcatgat caggttgaaa
      301 ctctgcaggc gatccggcat cggcacgcgg gcgtgccgta cataaccggt ggcctgcttg
      361 attccgggga tgcggcgcaa ggggctgcca tcttcgcaca gcggttcgat cgctcgccgc
      421 atgcctggcg ggaggtggtg gtagaactcg aagagccgtt gcatcgcgta gcgcgaattg
      481 cccccgaaca gttcatcgcc accgtcgccg gcgagcatcc tggtgcagcc gtcggcctgc
      541 gcgatcctgg cgcagtaata ggccggcagt gcggaagagt tgccgaaggg ttggtcgtga
      601 tgctgcgcga ccgccggaat gctgtccagg aggtccgccg gcgtcacgta atattcgtgg
      661 tgttcgcagc cgaagtgagt cgcggcgagg cgggcgtact ccatttcgtc ataaccctcg
      721 gcgtcgaagc cgatcgagta tacgggcgct ggtttgccgg tgatctggcc gagcatgccg
      781 gccaccgtcg agctgtcggt accgcccgac aggaaggcgc cgacgcgttg gtgacccgtg
      841 atctgctcgg cgacgctctc gcggatcagc tcgcggaagc cgtcgcgtgc cggggcaaag
      901 gattgacggt gttgctcgtc gaactgcagt ggccagtggc gctgcacgtg cgcaccgttg
      961 cggtcgacca gcagcgaatg cgccgccggc agccggcgta cctgctggaa gatggtgcgc
     1021 ggcgcaggaa tcatgtggaa gtacagataa tcgaaaatcg cctgtggatc aagttcgccg
     1081 ccgtgtcccg gaacgcggtc agcgcgatcg gcgaaggcga gcacctggcc atcggcccga
     1141 taacacagcg tctcgatggc gaagcggtcg gtagccagga aggcacactt gcgcgccgaa
     1201 tcgacaatgg ccagggcgta ggatccgtgc agttgcgagg cgatgtcgac cccgctgcgg
     1261 tgccaggctt tcagaagttc cgtggccaga ccgccgggta gcgagatgcc tgcggcactt
     1321 ccgtccctga aagaggggga gccgatgacc gcgcagatgg cctctgcgtc gcgtgcgaca
     1381 tcatcgccct gcaacgccag tgtcctgatg gaaagcggcg cagcgccggt gccgaagagg
     1441 cctgccagat caatctggcc agagagtcta tctttcatca tgaggtgtgt tgggggcagt
     1501 ttatgggccg acaggatgct acgctaaagc agtgactctt gttgtgtcag atttcactct
     1561 caagcctgat acacttttca gagatggcgg aggcgtcgat gtccctggtt gccgctcccg
     1621 gtcgcgccac gcagagacga aagaccgctt ttgttgaaaa ccttgccgtt tcatccactc
     1681 catgccagag gcctgagtct cctgctgctg ctcgcggcgg ccgcactggg ggtgttcatt
     1741 gc
//
LOCUS       DAOSIT010000533         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_59207_length_1741_cov_11.089561, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000533 DAOSIT010000000
VERSION     DAOSIT010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59207_length_1741_cov_11.089561"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PLL14_10725"
     CDS             complement(<1..1726)
                     /locus_tag="PLL14_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOG04446.1"
                     /translation="MWTARRKDGVPVADADIVWSDGVGVLKSGRTAADGVARLAAASP
                     ETSYVYGQDPAGGVFISENFYHDSEIYNAKIYAVTDRPLYRPGDLVQVKFLGREFRSA
                     RRSEPVASSDISVTVVDPAGTEVLTRKLRYDSAKGGETEFQLPAAAQAGGWELVFARG
                     EDSYGAAFRVADYVKPHFEVDVVADKSEFKTREAVTGRIRLSYPDGKPVVGALVRLTA
                     RAQPLTMVEGELRYGGQFPVAIKADELKTGSDGVAAFSLPPADDPARYILSLLAQDGA
                     AYRVRVTKEILVERGATLWQLAAPAYFSRPGEDVDFRYLPQGNTSPASRPARWEILRL
                     EDRQRREGSLTEGKSTPAAGENTPAGGDSIRLAFPDPGSYTVSLRDARGNLLAATSHW
                     VSGEGMKAIPGSVEIVFDKEQYQAGETAHALITFPQAVGDALLTLERDQVEAHALLSG
                     KASWLNVSQLAPAQWRADIPVKEEYGPNVTFSVLYLANGDYVFQNKGLRVAVPVVDIA
                     IRTPRERYAPGETVTLELETRTGGRPTPALVNLGVVDEMIYVLQPEIAPSIGNFFYHP
                     RRNNVRTTV"
BASE COUNT          289 a          559 c          578 g          315 t
ORIGIN      
        1 tgacggtcgt acgcacgttg ttgcggcgcg ggtgatagaa gaagttgccg atgctgggcg
       61 cgatttcggg ttgcagcacg tagatcattt catcgacgac gccgaggttg accagcgccg
      121 gtgtcggccg cccgccagtc cgggtttcca gttcgagagt gaccgtttct cccggcgcgt
      181 agcgttcgcg cggcgtgcgg atggcgatat cgacaaccgg cacggccacg cgcaggcctt
      241 tgttctggaa aacgtagtcg ccattggcga ggtagaggac ggaaaaggtg acattcggac
      301 cgtattcttc cttgaccgga atgtccgcgc gccactgcgc tggcgccagc tggctgacgt
      361 tcagccagct ggccttgccg ctgagcagtg cgtgcgcctc gacctggtca cgctccagcg
      421 tcagcaaggc atcaccgacc gcctgcggga aggtaatcag cgcgtgcgcg gtttcaccag
      481 cctggtactg ttccttgtcg aagacgatct cgacactccc cggaatcgct ttcatgcctt
      541 cgccggaaac ccagtggctg gtcgccgcca gcagattgcc tctcgcgtcg cgcagactga
      601 cggtatagga accgggatca gggaaagcga ggcggatact gtcgccaccg gcgggcgtgt
      661 tctctcctgc ggcgggcgta cttttaccct cagtcagaga tccttcgcgc cgctgccggt
      721 cctcgaggcg gagaatctcc cagcgcgccg ggcggctggc cgggctggtg ttgccctgtg
      781 gcaggtaacg gaagtcgacg tcttcgccag ggcgcgagaa ataggccggg gcggccagtt
      841 gccagagcgt ggcgccgcgt tcgacgagga tttccttggt cacccgcacg cgataggcag
      901 cgccatcctg ggccagcaga ctcaggatgt agcgagccgg atcatcggcc ggcggcaggc
      961 tgaacgccgc cacgccatcg ctgccggttt tcagttcgtc ggccttgatg gcgaccggga
     1021 actgaccacc atagcgcagt tcgccctcga ccatggtcag cggctgtgcc cgtgccgtca
     1081 gacgcaccag cgcgccgacc accggcttgc cgtcgggata gctgagacga atccgcccgg
     1141 tcacggcttc cctggtcttg aactcgctct tgtcggcgac cacatcgacc tcgaagtgcg
     1201 gcttgacgta gtcggcaact cgaaaagccg cgccgtagct gtcttcgccc ctggcgaaga
     1261 cgagttccca gccgccggcc tgggcggccg cgggcaactg gaactccgtt tcgccgccct
     1321 tggccgagtc gtagcgcagc ttgcgcgtga ggacttcggt ccccgccgga tcgacgacgg
     1381 tgacgctgat gtcgctcgaa gcgaccggct ccgaacgacg ggcactgcga aactcacggc
     1441 ccaggaactt gacctgcacg aggtcgccag gacgatacag cgggcgatcg gtgacggcgt
     1501 agatcttggc gttgtagatt tcgctgtcgt ggtagaagtt ctccgagatg aagacgccac
     1561 cggcggggtc ctggccgtag acgtaactgg tctcgggact ggcggcggcc aggcgggcaa
     1621 ctccgtcggc agcagtcctg ccgctcttca ggacgccgac gccgtcgctc cagacaatat
     1681 cggcgtcggc gaccggaaca ccgtccttgc gccgagcggt ccacaccagc atctcggtgg
     1741 c
//
LOCUS       DAOSIT010000534         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_59321_length_1739_cov_8.555819, whole genome shotgun sequence.
ACCESSION   DAOSIT010000534 DAOSIT010000000
VERSION     DAOSIT010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59321_length_1739_cov_8.555819"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PLL14_10730"
     CDS             <1..1338
                     /locus_tag="PLL14_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit TatB"
                     /protein_id="HOG04447.1"
                     /translation="RRNAARNGTSIRLTYHLQRLRQLLQRSEQLLDILVDLQPRSSDS
                     VAYPPMVSLFTQLVRDECLRNNLYRHWRQNTELIALRVTDNASHHGEHYITETRQEYW
                     SMARSATIGGFVIAFMACLKIVLAGAQMPPLTGAIAFCLNYGIGFCLIHILHGTVATK
                     QPAMTANAIAASISEAGGKLRDIEALTVLITRTCRTQVIAILGNVCIAIPLSALIAFA
                     VFSSSGQHFTSPEKSLHLLKEQSLVDSGAVFYAAIAGVCLFISGLISGYYDNYAAYNR
                     IPERILQLAWPRRLFGERRMQRIAAYIGDNLGALAGNLLFGFLLGGTTLFGVLLGLPI
                     DIRHVAFSSAFVGIAMAGLDFAPDLRLLVWAVLGVAGIGFLNLAVSFALALNVALRSR
                     QVSDSQWRTLVRTVLRNLRRQPRDFFLPPKEANACLVLQTPANTGDQSIHPDH"
     gene            1465..>1739
                     /locus_tag="PLL14_10735"
     CDS             1465..>1739
                     /locus_tag="PLL14_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04448.1"
                     /translation="MTEPKSTTKKPPAAAKKVATKETTAAAPVKAPAAAKRTPVKKAV
                     STPATDSAPIAPPEPQIPATSEAAPKPSTEERYLMVQSAAYFIAEKDG"
BASE COUNT          334 a          603 c          478 g          324 t
ORIGIN      
        1 cgcaggaatg ccgcccgcaa cggcaccagc atccgcctga cctatcatct gcaacgcctg
       61 cggcaactgc tgcagcgcag cgagcaactg ctcgacattc tggtcgatct gcagcccagg
      121 tcgagcgaca gcgtggctta cccgccgatg gtcagcctat tcacccagtt ggtgcgcgac
      181 gagtgcctgc gcaacaatct ctaccgacac tggcgccaga ataccgaact gattgccttg
      241 cgggtgaccg acaacgccag ccaccatggc gagcactaca tcaccgagac acgccaggaa
      301 tactggtcga tggcacgttc ggcgacgatc ggcggcttcg tcatcgcctt catggcctgt
      361 ctgaagatcg tcctggccgg tgcgcagatg ccaccgctga ccggcgcgat cgccttctgc
      421 ctcaactacg ggatcggctt ttgcctgatt cacatcctgc acggcaccgt cgccaccaag
      481 cagccggcga tgaccgccaa cgccattgcc gcgagcatca gcgaagccgg tggcaaactg
      541 cgcgacatcg aagcgctgac cgtactgatt acgcgcacct gccgtaccca ggtcattgcc
      601 attctcggca acgtctgcat cgccatcccg ctctcggcac tgattgcctt cgcggtgttc
      661 agcagcagcg gccagcactt caccagtccc gagaagtcgc tgcatctgct caaggagcaa
      721 agtctggtcg atagcggggc ggtcttctac gcggctattg ccggagtctg tcttttcatt
      781 tcgggtctga tcagcggcta ctacgacaat tacgccgcct acaaccgaat tcccgaacgc
      841 atcctgcaac tcgcctggcc gcggcgcctg ttcggcgaga ggcgcatgca acgcatcgcg
      901 gcgtacatcg gcgacaacct cggggctctc gccggcaacc tgctgttcgg attcctgctt
      961 ggcggcacga cgctgttcgg cgtgctgctg gggctgccga tcgatatccg ccatgtcgcc
     1021 ttctcatcgg ctttcgtcgg tatcgcgatg gccggtctcg attttgctcc cgacctgcgc
     1081 ttgctggtct gggcggtact gggcgtagcc ggcatcggtt tcctgaacct ggcggtcagt
     1141 tttgcactgg cgctcaatgt ggcgctgcgc tcccggcagg tttccgactc gcaatggcga
     1201 accctggtcc gtacggtgct gagaaacctg cgtcgccagc cccgcgattt cttcctgccg
     1261 cccaaagaag ccaacgcctg cctggtcctg caaaccccgg caaacacggg cgaccaatca
     1321 atacaccctg atcactgaga tgggtattcg ccttgaccgg caggcccgat tttcctatcc
     1381 tgctgtctga tttcagcgtc ggctgaaccg ttccagcgct tgcccaccct ccataccatc
     1441 gacaccattc ccaggaaacc gatcatgacc gaacccaaga gcacgaccaa gaaacccccc
     1501 gccgccgcca agaaggtggc gacgaaagag acgactgccg ctgcgccagt caaggcccct
     1561 gctgctgcga agcgaacccc tgtcaagaag gcagtgtcaa cgccggccac cgacagcgcc
     1621 ccaatcgcac cgcccgaacc gcaaataccc gccaccagcg aggcggcgcc caagccaagc
     1681 accgaggaac gttatctgat ggtccagtct gccgcgtatt tcattgccga gaaggacgg
//
LOCUS       DAOSIT010000535         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_59501_length_1735_cov_5.083333, whole genome shotgun sequence.
ACCESSION   DAOSIT010000535 DAOSIT010000000
VERSION     DAOSIT010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59501_length_1735_cov_5.083333"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="PLL14_10740"
     CDS             <1..>1735
                     /locus_tag="PLL14_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG04449.1"
                     /translation="GEVARLLRAAQAGRITLGERALAPGDIAILVRSHAQGRLLRDAL
                     LQLGIGSVELSQQSIFHTADAEDLERVLLAILEPARPSLLYSALATELMGVDAAAVAA
                     LAADEMGLLQTMARFSGYRETWLRRGFGVMLRAWMASEAVALRLLARNDGERRLTNLL
                     HLGELLQQTSADQPAPDALLRWLASQRSDRGADEVAQLRLESDRNLVQIVTIHKSKGL
                     EYGIVFCPFLWDGHQPSFGEFEGKEYHDDDGLPVIDFRSDLDPVEADAITRRRREEND
                     AEFMRLLYVALTRAAYRCYVVAGCYATLSFGRPSMAQGNRSLLNWLVAGKDMRYADWL
                     THQRPPEEIEEAWQTLARTAATSLSLRDLPSETPTPLAGVGLSPASLTALAAPANIAG
                     GWRIGSFSSLQHGAESEASASDHDGRSLTGLPAVGVGAAAPAEIAPDDILYFPRGPAA
                     GDCVHALFERIDFTDPGRWPEIIDEVLASHPLSLPGLPDGESGRRLAHMLRRLLDDVL
                     ATRLPGDIVLSSVARRQRLVELGFNLPAAGLTAPALNGWLTAHGYNQPRLGFAPLDGY
                     LKGFIDLVFCQA"
BASE COUNT          274 a          616 c          579 g          266 t
ORIGIN      
        1 ggcgaagtgg cgcgcctgct gcgcgcggcg caggccggcc ggatcacgct cggggaacgc
       61 gccctggcgc ctggcgacat cgccatcctg gtcaggagcc atgcccaggg gcgcctgctg
      121 agggacgcgc tgctgcagct tggtatcggc agcgtcgagc tctcgcagca aagcatcttc
      181 cacactgccg acgccgagga tctggagagg gtgctgctgg cgatcctcga accggcgcgc
      241 ccctcgctcc tctacagcgc gctggcgacc gaactgatgg gtgtcgatgc cgccgccgtc
      301 gctgcgctgg ctgccgacga gatggggctc ctgcagacca tggcgcgctt cagcggctat
      361 cgggagacct ggcttcgccg cggcttcggg gtcatgctgc gcgcctggat ggcgagcgaa
      421 gcggtcgccc tgcgtctgct ggcgcgcaac gacggcgaac ggcggctgac caacctgctg
      481 cacctcggcg aactgctgca acagaccagt gccgaccagc cggcgccgga cgcgctgctg
      541 cgctggctcg ccagccagcg cagcgaccgg ggcgccgacg aggtcgccca gttgcgcctc
      601 gagtccgacc gcaacctggt gcagatcgtc accatccaca aatcgaaagg cctcgaatac
      661 ggcatcgtct tctgcccttt cctctgggat ggacaccagc caagctttgg cgagttcgag
      721 ggcaaggagt accacgatga tgacgggctg ccggtgatcg acttccggtc cgacctggat
      781 ccggtcgaag cggacgcgat cacgagacgg cgacgcgagg agaacgacgc cgagttcatg
      841 cgcctgctct acgtcgcgct gacgcgagcg gcgtaccgct gctacgtggt tgccggctgc
      901 tacgcgaccc tgtccttcgg tcggccatcg atggcccagg gcaatcgcag cctgctcaac
      961 tggctggtcg ccggcaagga catgcgctat gcggactggc tgacgcacca gcgaccgccg
     1021 gaggaaatcg aagaagcctg gcagacgttg gcaaggacgg cggcgacgtc gttgtcgctg
     1081 cgcgacctcc cgagcgagac gccgacacca ctcgccggcg tcggcctgtc gccagcgagc
     1141 ctgaccgcgc tggccgcacc cgcgaacatc gccggcggct ggcgaatcgg cagtttcagc
     1201 agcctgcagc atggcgccga gagcgaagct tcggccagcg accacgacgg tcgctcgctc
     1261 accggcctgc cggccgtcgg cgtcggcgcc gccgcgccgg cggaaatcgc gccggacgac
     1321 atcctctact tcccgcgcgg accggccgcc ggcgattgcg tgcatgcgct cttcgagcgc
     1381 atcgacttca ccgatccggg ccgctggcca gagatcatcg acgaggtgct ggccagccat
     1441 cccctctcgc taccgggact gccggatggc gaatccggcc gccgcctggc acacatgctg
     1501 cgccgactgc tcgacgacgt tcttgcgacc cggctgcccg gcgacatcgt cctgtcctcg
     1561 gtggcgcgcc ggcaacgtct ggtcgaactg ggcttcaacc tgcccgccgc agggctcacc
     1621 gcaccggcac tcaacggctg gctgacagca cacggctaca accagccgag acttggcttt
     1681 gccccgctcg acggttacct caagggcttc attgatctgg tgttctgcca ggcaa
//
LOCUS       DAOSIT010000536         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60011_length_1725_cov_7.210778, whole genome shotgun sequence.
ACCESSION   DAOSIT010000536 DAOSIT010000000
VERSION     DAOSIT010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60011_length_1725_cov_7.210778"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="PLL14_10745"
     CDS             <1..100
                     /locus_tag="PLL14_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOG04450.1"
                     /translation="GEQGAREAGKMRAEGKEYIVKDGDVLNFLFNV"
     gene            128..850
                     /locus_tag="PLL14_10750"
     CDS             128..850
                     /locus_tag="PLL14_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOG04451.1"
                     /translation="MPRPSDPRRQEFVAGIRDTFPLLLGALPFGLIYGALAATSGLSM
                     AAAIAMSALVFAGSSQFIAVGLVAAQTPVAIIVLTTLIVNLRHMLYSATLLPYLRNLP
                     QKWRIPLAFWLTDETFAVTVHRFQKDDASAFKHWYQFGSSIAMYLNWQFWCFLGLVLG
                     NRIPDAQSWGLDVAMPVTFIGMIIPFVKTIPIAVCVLTAGAASLLTLSLPYKLGIVVS
                     AFAGITSGLLAQRLTQKGNGRR"
     gene            847..1167
                     /locus_tag="PLL14_10755"
     CDS             847..1167
                     /locus_tag="PLL14_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOG04452.1"
                     /translation="MSAREAWMLAGMMIVTFGIRYFLFALADRFRMPPWIEGALKFIP
                     PAVLTAITLPAVLLPKGEWFLSWENPYLVAALLGLAAGVLTRNLLATIAIGLAAFFVH
                     RLFL"
     gene            complement(1226..>1725)
                     /locus_tag="PLL14_10760"
     CDS             complement(1226..>1725)
                     /locus_tag="PLL14_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG04453.1"
                     /translation="VAHFGWLTPTQMIDGLALGETTPGPLIMVVAFVGFVGGYGKAFF
                     GPESLFLAGAMAASLVTWFTFLPSFVFILAGGPLVESTHNDLKFTAPLTAITAAVVGV
                     ILNLALFFGYHVLWPKGLSGTFDWVSALIAVAAGVALFRYKLNVIPVIAICAVTGLLV
                     RMVGG"
BASE COUNT          328 a          575 c          506 g          316 t
ORIGIN      
        1 gggcgagcag ggagccaggg aggccgggaa aatgcgcgcc gaaggcaagg aatacatcgt
       61 caaggacggc gacgtgctga actttctgtt caacgtctag agcgcccgcc caggagtccg
      121 ccaggccatg cctcggccca gtgatccgcg gcggcaggaa ttcgtcgccg gaatacggga
      181 caccttcccg ctcctgctcg gggctctgcc gttcggactg atctacggcg ccctcgccgc
      241 gacttcgggt ctgtcgatgg cggcggcaat cgccatgtct gcgctggttt ttgccggatc
      301 gtcgcagttc atcgccgtcg gtctggtggc cgctcagacg ccggtcgcga tcatcgtact
      361 gacaaccctg atcgtcaacc tgagacacat gctttacagc gccaccctgc tgccctacct
      421 caggaatctg ccacagaaat ggcggattcc gctggcgttc tggctgaccg acgaaacctt
      481 cgccgtcacc gtgcatcgct ttcaaaagga cgatgcctcc gccttcaagc attggtacca
      541 gttcggctcg tcgatcgcca tgtatctcaa ctggcagttc tggtgctttc ttggcctggt
      601 gctcgggaac cgcattcccg acgcgcagag ctgggggctc gacgtggcaa tgccggtcac
      661 cttcatcggc atgatcatcc cgtttgtcaa gaccatcccc atcgcggtct gcgtactgac
      721 ggccggcgcc gcctcgctgc tcaccctgag cctgccctac aaactgggga tcgtcgtttc
      781 cgcttttgcc ggcatcacct cgggcctgct cgcgcagcgc ctcacgcaga aagggaacgg
      841 ccggagatga gcgcgagaga agcgtggatg ctggccggga tgatgatcgt caccttcggg
      901 attcgctatt ttctctttgc cctggccgac cgattccgga tgcctccttg gatcgaaggc
      961 gcactgaaat tcattccgcc ggccgtgttg acagccatta ccctgcccgc agttctcctg
     1021 ccgaagggcg aatggttcct gtcgtgggaa aacccgtatc tggtcgccgc cctgctcggc
     1081 ctggccgccg gggttctgac acggaatctg ctggcgacca tcgccatcgg cctggccgcc
     1141 ttctttgtgc atcggctttt tctgtgaatc accgacagcc gcccggttca ccgaccgagc
     1201 agatccctac caagcaattc gaccatcagc cgccgaccat tctcaccagc agacccgtga
     1261 ccgcgcagat ggcgatgaca gggatgacgt tcaacttgta acgaaaaagt gcgacgcccg
     1321 ccgcaacggc gatcaacgcc gacacccagt cgaaggtgcc ggacaggcct ttgggccaca
     1381 acacatggta gccgaagaac agcgccaggt tgaggatcac gccgacgacc gccgccgtga
     1441 tcgcggtcag cggcgcggta aatttcagat cgttgtgggt cgactcgacc aaaggcccgc
     1501 cggccaggat gaacacgaac gacggcagga aggtgaacca ggtcaccagc gacgccgcca
     1561 tagccccggc aagaaacagc gactccgggc cgaagaacgc cttgccgtag ccgccgacga
     1621 agccgacaaa agccaccacc atgatcagcg gccccggcgt cgtttcaccg agggccaggc
     1681 catcgatcat ctgcgtcggc gtcagccacc cgaaatgcgc caccg
//
LOCUS       DAOSIT010000537         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60163_length_1722_cov_9.190162, whole genome shotgun sequence.
ACCESSION   DAOSIT010000537 DAOSIT010000000
VERSION     DAOSIT010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60163_length_1722_cov_9.190162"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1722
                     /locus_tag="PLL14_10765"
     CDS             <1..>1722
                     /locus_tag="PLL14_10765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG04454.1"
                     /translation="FNDMLDQLDHSRRIEQELAAQSRAAAAQRQLLEAVPIPLMVTAT
                     PHHQVLHTNAQAQAWLDGRLSDPWVTGLSPAARVRFFQLLADTGAANEFEVLWHGEKR
                     TDWALLSARRLRYQDQDAVLTAFTPIGRIKHMEERLELWAKVFEASSESILITDANRQ
                     ILTVNRAFCRNTAYERIEVIGEQPNFLRSDRHPHGFYDDLWQSASMRGSWQGEMWIRR
                     KTGEAFPVWAVLNAVRDDDARITHYIATWFDISARKADEQRISHLAQHDVLTDLPNRA
                     LCVERLRVALQQAERHQRRVAVLFIDLDRFKNINDSLGHHIGDGVLRSVAHQLQCAVR
                     TGDTVSRMGGDEFVVILNDVADVEEIRQIVDNRLIRLIRQPHNVDGAELHVSCSVGIA
                     VYPEDGRELDILMRNADAAMYQAKEQGRNNAQFFTIEMDRRARERMQIENDLRGGIER
                     GELRVYYQPRVDCRSGTLLGAEALVRWQHPQHGLMTPANFIPVAEESGLIIPLGTWVL
                     AAACRQQVRWRESGFADIVISVNLSAGQLHDPELLATLQSAITEHGVNPAMIELELTE
                     SLLMEDV"
BASE COUNT          336 a          564 c          539 g          283 t
ORIGIN      
        1 ccttcaacga catgctcgac cagctcgacc acagccgccg catcgaacag gaacttgccg
       61 cgcaatcacg cgcagccgcg gcacagcgac aactgctcga agccgttccc attccactga
      121 tggtcaccgc cactccgcat caccaggtcc tgcataccaa tgcgcaggcc caggcctggc
      181 tcgacggtcg tctgagcgat ccctgggtga ccgggctgtc acccgcggcg cgtgtgcgct
      241 tcttccagtt gctggccgat accggcgcgg ccaatgaatt cgaagtgttg tggcatggcg
      301 agaaaagaac cgattgggcg ctgctttcgg ctcgccgcct gcgctaccag gaccaggacg
      361 cggtcctgac ggcattcacc ccgatcggcc gcatcaagca catggaagaa aggctggaac
      421 tctgggccaa ggtgttcgag gcctcgtccg agagcatcct gatcaccgac gccaaccggc
      481 aaatcctgac tgtcaaccgc gctttctgtc gcaacacggc ctatgagcgg atcgaggtga
      541 tcggcgaaca acccaacttc ctgcgcagcg accgccaccc gcacggcttc tacgacgacc
      601 tctggcaatc cgcgagcatg cgcggctcct ggcagggcga gatgtggatc cggcgcaaga
      661 ccggggaggc gttcccggta tgggcggtat tgaatgcggt gcgtgacgat gacgcccgga
      721 tcacgcacta cattgcgacc tggttcgaca tcagcgcacg caaggccgac gagcaacgca
      781 ttagccatct cgcgcagcat gacgtgctga ccgacctgcc gaatcgggcg ctctgcgtcg
      841 agcgcctgcg ggtcgccctg cagcaggcgg agcgccacca gcgacgcgtc gcggtgctgt
      901 tcatcgacct cgaccgcttc aagaacatca atgattcact cggccatcac atcggcgatg
      961 gcgtcctgcg ttcggtggcg caccaattgc agtgcgcggt tcgcaccggc gacacggtca
     1021 gtcgcatggg cggcgacgag ttcgtggtga ttctcaacga tgtcgccgac gtcgaggaaa
     1081 tccgccagat cgtagacaac cgcctgatcc ggctgatccg tcaacctcac aacgtggacg
     1141 gggccgaact gcacgtttcc tgcagcgtcg gcatcgccgt ctatccggag gacggccgcg
     1201 agctcgacat cctgatgcgc aacgccgacg cggcgatgta ccaggccaag gagcaggggc
     1261 gcaacaacgc gcagttcttc accatcgaga tggaccgacg ggcacgcgag cggatgcaga
     1321 tcgagaatga tctgcgcggc ggcatcgagc gtggggaact gcgggtctat taccaaccgc
     1381 gcgtcgactg ccgcagcggc acactgcttg gtgccgaagc cctggtccgc tggcagcatc
     1441 cgcagcacgg cctgatgacc ccggcgaact tcattccggt ggccgaggaa agcggcctga
     1501 tcatcccgct gggaacctgg gtgctggcgg cagcctgtcg ccaacaggtg cgctggcgcg
     1561 agagcggctt cgccgacatc gtgatctcgg tcaatctctc ggcggggcaa ctccacgacc
     1621 cggagttgct ggccacgctg caaagtgcca ttaccgagca cggggtgaac ccggcaatga
     1681 tcgaactgga gctgaccgaa tcgctgctga tggaggatgt ga
//
LOCUS       DAOSIT010000538         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60165_length_1722_cov_8.233953, whole genome shotgun sequence.
ACCESSION   DAOSIT010000538 DAOSIT010000000
VERSION     DAOSIT010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60165_length_1722_cov_8.233953"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLL14_10770"
     CDS             complement(<1..295)
                     /locus_tag="PLL14_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HOG04455.1"
                     /translation="MNLEKVVFGFFIVLAATLNFGFFIGEIDQPRHHHVYELFAAIVV
                     NLIATVLKFGDRTQIGAVHLSTSLVADLQLVAAACVWAIAVHVTGEGMSADVTT"
     gene            complement(554..1573)
                     /locus_tag="PLL14_10775"
     CDS             complement(554..1573)
                     /locus_tag="PLL14_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOG04456.1"
                     /translation="MGTFTNNSDNCVNANGVVRPPRRLLDQLSESIRLKHYSIRTEQA
                     YRGWVRRFILFHGKRHPQEMGACEVEAFLTHLAVEGRVSASTQNQAKSALLFLYKEVL
                     RTDLPWLDDVESAKSGKRLPVVLTPEEVQRLLGPIDGTTGLILRLLYGTGMRMMECLR
                     LRVKDVDFARREILVREGKGFKDRVTMLPERLVAPLGEHLARVKALHAADLAAGFGDV
                     YLPWALERKYPNAAREWAWQYVFVAARRSVDPRSGAVRRHHVEAQSIQRAMRQALRLA
                     GVSKPATPHTLRHSFATSLLETGYDIRTVQELLGHADVSTTMIYTHVLKRGGRGVRSP
                     LDALG"
BASE COUNT          331 a          568 c          493 g          330 t
ORIGIN      
        1 tggtcgtgac atccgcggac attccctcgc cggtgacatg cacggcgatc gcccacacac
       61 aggccgccgc caccagttgc aaatcggcga ccaggctggt cgacagatgc accgcgccga
      121 tctgcgtccg gtcaccgaat ttgaggaccg tggcgatcag gttgacgacg attgcggcga
      181 acagctcata gacatgatga tgcctcggct ggtcgatctc gccgatgaag aaaccgaagt
      241 tcaaggttgc cgcgagcacg ataaagaaac cgaaaaccac cttttccagg ttcatgagcc
      301 cccccgagcg ttcgcgccac ccctgcggca gcggcctgac cccgaagcat acgcatcctg
      361 gcgtgtgccg gcaacggcag gggcttgtcc ggcgccgtag ctgcgctggc cgtgcacaag
      421 catgcttgct tccttgcgac cgaggcattg atcccggcga tccagcgttc gctcatttgt
      481 tcgctcattt tcagtcagac tgtcccgaga atcgaggcat cgatcctgtt atcggctgca
      541 cgggccgatg gttctacccc agcgcgtcga gcggactgcg aacaccgcgc ccaccgcgct
      601 tgagcacgtg cgtgtagatc atcgtcgtgc tcacatcggc atggcccagc agttcctgta
      661 cggtgcgaat gtcgtagccg gtttccagaa gggacgtggc gaacgaatgg cgcagagtgt
      721 gcggcgtagc cggcttgctg actcccgcca gccgtagcgc ctgacgcatc gcacgctgga
      781 tactctgcgc ctccacgtga tgtcgccgca ccgccccgga acgtggatcg accgatcgtc
      841 tggcggcaac gaagacgtac tgccacgccc actcacgcgc cgcgttcggg tatttccgct
      901 ccagagccca tggcaggtac acgtcgccaa aacccgcagc cagatccgcc gcgtgcagcg
      961 ccttcacacg tgccagatgc tcccccagcg gcgccaccag cctctccggc agcatcgtca
     1021 cccggtcctt gaagcccttt ccctcgcgca ccaggatttc gcggcgagcg aagtccacgt
     1081 ccttaacgcg cagtcgcagg cactccatca tgcgcatccc cgtaccgtaa agcagccgga
     1141 ggatcagccc cgtggttccg tcgatcggcc ccagcaggcg ctgaacttct tcgggcgtca
     1201 gcactacggg cagacgcttg ccggacttcg ccgactcgac atcatccagc caggggagat
     1261 cggtcctcag cacttccttg tagagaaaca gcagagcgct ctttgcctga ttctgcgtgg
     1321 atgcggaaac ccgcccctcc accgcgaggt gggtgagaaa cgcttcgacc tcgcatgcgc
     1381 ccatttcctg cggatgtcgc ttgccatgga aaaggatgaa acgtcgcacc cagcccctgt
     1441 aagcctgctc cgtccgtatg ctatagtgct tgaggcgtat gctttccgag agttggtcaa
     1501 ggagccgcct gggaggccgg acgacgccat tggcattgac gcagttgtcg gaattattag
     1561 tgaacgtgcc catactctac ccccttgttt ccgttggaag ttcagccatg catgtacatg
     1621 catacagtat agtgacgcaa agcactgcgt caagtaggat tgctacatca cgttgaacta
     1681 aaccgctggc gcggtggtgg ttagacccac cgccgcgttg ct
//
LOCUS       DAOSIT010000539         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60265_length_1720_cov_9.948949, whole genome shotgun sequence.
ACCESSION   DAOSIT010000539 DAOSIT010000000
VERSION     DAOSIT010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60265_length_1720_cov_9.948949"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..947
                     /locus_tag="PLL14_10780"
     CDS             15..947
                     /locus_tag="PLL14_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOG04457.1"
                     /translation="MAILVIAEHDNTSIKAATLNAVTAATKIGGDIHLLVAGSACAAA
                     AEAATRIAGVSKVLVADAGHYADQSPENLAALVVANAAAYACLVAGATTIGKNFMPRV
                     AALLDVAQISEIVAVESPDTFVRPIYAGNVVATVRSTDPIKVITVRATAFEAAGEGGS
                     AAIESLPAGPDLAKSRLVGRELTKSERPELTAAKVIVAGGRGLGSGENFHQLLEPLAD
                     KLGAALGASRAAVDAGFVPNDYQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDAEAPIFQVADYGLVGDLFDVLPALLKELG"
     gene            973..1632
                     /locus_tag="PLL14_10785"
     CDS             973..1632
                     /locus_tag="PLL14_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOG04458.1"
                     /translation="MNLPDGLLDGGWTAWLLFVAGVGLAAWRAPWHVLADSNRLHVFL
                     GTIVALLLLWHLKAGVKPGLSLHLLGATFLTLCYGWALAGIGLCLVLVGASLNGTAGW
                     QAFAVNALLMAGVGVAASHAVHRLVDRMLPRHMFVYIFGKGFFAAALTVFAVGAASCL
                     LLTLAGAYSGEYLSSEYLPYFFLLGFSEAWLSGMLTTLLAVYRPDLLADFDDAQFLGR
                     K"
BASE COUNT          280 a          520 c          560 g          360 t
ORIGIN      
        1 aaggtgatct gaccatggcc attctcgtta ttgctgaaca cgacaatacc tcgatcaagg
       61 cggctacgct caatgcggtc acggcggcga cgaagatcgg tggcgacatt cacctgctgg
      121 ttgccggttc cgcctgcgcg gcagcggccg aggcggctac ccggatcgcc ggagtcagca
      181 aggttctggt tgccgatgcc gggcattatg ccgaccagtc accggagaac ctggcggcgc
      241 tggtcgtcgc caatgccgcc gcgtatgcct gcctggttgc cggggcgacc accatcggca
      301 agaacttcat gccgcgcgtg gcggcactgc tcgacgtggc gcagatttcc gagatcgtcg
      361 ccgtcgaatc gcctgatacc ttcgtacgcc cgatctacgc cggcaacgtc gtcgccactg
      421 tccgatcgac cgacccgatc aaggtcatta ccgtaagggc aacggccttc gaagcggcag
      481 gtgaaggagg cagcgccgcc atcgaaagtc tgccggccgg ccccgacctc gccaagagca
      541 ggctggtcgg tcgcgagttg accaagtcgg agcgtcccga actcaccgcc gccaaggtca
      601 tcgtcgccgg cggtcgtggc ctgggtagcg gcgagaactt tcaccagctg ctggaaccgc
      661 tggccgacaa gctcggcgcc gcactcggtg cctcgcgtgc agcggttgac gccgggttcg
      721 tgccgaacga ctatcaggtc ggtcagacgg gcaagatcgt cgcgccgcag ctctacatcg
      781 cggtcggcat ttccggggcc atacagcacc tggcgggaat gaaggacagc aaggtgattg
      841 tggcgatcaa caaggacgcc gaggcaccga tcttccaggt cgccgattat ggtctggtcg
      901 gtgacctctt cgatgtcctg ccggcattgt tgaaggaact gggctgacca gcaacgggct
      961 gcgcgcggcc acatgaacct gccggacggc ctgctggatg gtggctggac ggcctggctg
     1021 ctgtttgttg ccggagtggg tctggctgcc tggcgggcgc cgtggcatgt gctcgccgac
     1081 agcaaccgcc tgcacgtctt cctggggaca atcgtcgcgc tgctcctgct ctggcacctc
     1141 aaggccgggg tcaaacccgg cctttcgctg cacttgctgg gagcgacatt ccttaccttg
     1201 tgttatggat gggcgctggc cggcatcggc ctgtgcctgg tactggtcgg ggccagcctg
     1261 aacggtactg ccggttggca ggcctttgcg gtcaacgccc tgctgatggc gggcgtaggc
     1321 gtcgccgcga gccatgcggt acatcgactg gtggaccgaa tgctgccacg ccatatgttc
     1381 gtctatatat tcggcaaggg tttcttcgcc gcggcgttga cggtattcgc cgtcggtgcg
     1441 gcgtcgtgtc tgctgttgac gctcgcagga gcctacagcg gcgagtatct gagcagcgag
     1501 tatctgcctt atttcttttt gctcggtttt tccgaggcct ggttgtcggg aatgctgacc
     1561 acactgcttg ccgtctatcg acctgattta ctggcggatt ttgacgatgc gcagtttctc
     1621 ggcagaaaat gattgtcccg gaaccgtttc ctgtgtaact gtttggcacg acaaggtatg
     1681 ctttcccgat gggcttgaga ttcctgcgta gcgcgctgac
//
LOCUS       DAOSIT010000540         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60266_length_1720_cov_8.458258, whole genome shotgun sequence.
ACCESSION   DAOSIT010000540 DAOSIT010000000
VERSION     DAOSIT010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60266_length_1720_cov_8.458258"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLL14_10790"
     CDS             complement(<1..913)
                     /locus_tag="PLL14_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOG04459.1"
                     /translation="MMQRLTSALTLLLLSGLAFAGPAMDASGAKQATNWHAIIMFLIF
                     VAMTMGITYWAAGRTKSTADFYTAGGGITGFQNGLAIAGDYMSAATLLGLTAMTYTAG
                     YDGYIYMLAFFAGWPIILFLMAERLRNLGRFTFADITSYRLDQGKVRTMAAISSLVVV
                     VFYLIAQMVGAGQLIKLLFGLDYNIAIFVVGILMMVYVTLGGMVATTWVQIIKACMLL
                     FGGTLTLLLAYVQFGFSLTTLLEKAEAVHKLGPKLMAPGSLLADPVTAISLGLGLMFG
                     TAGLPHILMRFFTVGNAKEARKSVLYAS"
     gene            complement(910..1230)
                     /locus_tag="PLL14_10795"
     CDS             complement(910..1230)
                     /locus_tag="PLL14_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOG04460.1"
                     /translation="MSSVMYERMRVNPKFQKLVQERGRFAWTLATIVLVLFYGFFMLV
                     AFNPSLLGQKIAEGSMWPIGYTIELCLFVFFWLTTVIYVRRANGEFDDLTAELIKEAQ
                     KEKL"
     gene            complement(1227..1436)
                     /locus_tag="PLL14_10800"
     CDS             complement(1227..1436)
                     /locus_tag="PLL14_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04461.1"
                     /translation="MSVYAGRASFPASLFIPLRYPAAASIEQAVGSGLVFRAARGRSC
                     QNLVDTLQNPQQYRFNKTKPEDEKT"
BASE COUNT          395 a          504 c          512 g          309 t
ORIGIN      
        1 cggaagcgta gagcaccgat ttgcgcgctt ccttggcgtt gccgacggtg aagaaacgca
       61 tcaggatatg cggcagaccg gcggtaccga acatcagacc gaggcccagc gagatggctg
      121 tgaccggatc ggccagcagg ctacccggag ccatcagctt gggaccgagc ttgtgaacgg
      181 cttccgcttt ctcgagcagc gtcgtcaatg aaaacccgaa ctgaacgtac gccaggagca
      241 acgtcagcgt accgccgaag agcagcatgc aggccttgat gatctgcacc caggtcgtcg
      301 ccaccatgcc gccaagggtg acatagacca tcatcaggat gccgacgacg aagatcgcga
      361 tgttgtagtc gaggccgaag agcagcttga tcaactgccc ggcaccgacc atctgcgcga
      421 tcaggtagaa gacgaccacc accagcgacg agatcgcggc catcgtccgc accttgccct
      481 ggtcaagccg gtacgaagtg atgtcggcaa aggtgaaacg ccccaggttg cgcagccgct
      541 cggccatcag gaacaggatg atcggccagc cggcgaagaa ggccagcatg tagatatagc
      601 cgtcatagcc cgcggtgtag gtcatcgccg tcagcccgag cagggttgcc gccgacatgt
      661 agtcgccggc gatcgccaga ccgttctgga aaccggtgat gccgccgccg gcggtgtaga
      721 aatcggccgt ggacttggtg cgtccggcag cccagtaggt gatccccatg gtcatcgcca
      781 cgaagatcag gaacatgatg atcgcgtgcc agttggtggc ctgcttggcc cccgaggcat
      841 ccatcgccgg gccggcaaaa gccaggccgg aaaggagcag cagggtgagt gccgatgtca
      901 gtcgttgcat catagcttct ccttctgggc ttccttgatc aattccgcgg tcagatcatc
      961 gaattcccca ttggcgcgac ggacgtaaat caccgtcgtc agccagaaga agacgaacag
     1021 acaaagttcg atcgtgtagc caatcggcca catcgatccc tcggcaatct tctgtccgag
     1081 caggctcggg ttgaaggcga ccagcatgaa gaatccatag aaaaggacca gcacgatggt
     1141 tgccaacgtc caggcaaagc gtccccgctc ttgcaccagt ttctggaatt tgggattcac
     1201 ccgcattcgc tcgtacataa cactactcat gttttctcat cctccggttt tgttttgttg
     1261 aagcggtatt gctgcggatt ctgcagcgta tcgaccaggt tttgacaaga ccttcccctc
     1321 gccgcgcgga atactaaccc agatccaacg gcttgttcaa tcgaggctgc tgccgggtag
     1381 cgcagtggaa tgaacagcga ggccgggaaa cttgcccgac cggcataaac tgacatgcca
     1441 gaccggcatc cgtgtcgaac aagcgtgcgt gcgccatcgt gccgttgtcg cgccgcacaa
     1501 ttgcgattga caaaaaacga ctagaatcgc aagcgaaaaa gagacaacaa gggctggccg
     1561 tcaccgtgtg gcggcgaatg caccggagga gacaagatgc gggagatgac ttcttcccag
     1621 gcgagggata cctttcctcg cctgctgctg tatcacgcgc aggttcgcgg cgacaaaccg
     1681 gcgatgcgcg agaaggacct gggcatctgg cagtcctgga
//
LOCUS       DAOSIT010000541         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60385_length_1718_cov_11.224895, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000541 DAOSIT010000000
VERSION     DAOSIT010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60385_length_1718_cov_11.224895"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLL14_10805"
     CDS             <1..234
                     /locus_tag="PLL14_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG04462.1"
                     /translation="DRHAIRLRVFERGAGETLACGTGACAAVVAGIARGLLDSPVRVE
                     TRGGMLSIAWAGKGAPVLMTGPAVTVFKGEIEL"
     gene            231..884
                     /locus_tag="PLL14_10810"
     CDS             231..884
                     /locus_tag="PLL14_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOG04463.1"
                     /translation="MRSEEVAHYLRDNPGFFEEHIDLIANVVVPHPHAGRTISITERQ
                     MLALRDKNRQLEGKMGELLQFGEENDAISVKMHRLAVGLIAARSFQSLLHTLNFHLRD
                     DFAIPHVAVRLWHRPEGSDELPEFADVSRDLQAFAETLAQPYCGTTSGFETSGWFGEA
                     ATHVRSQALIAMRNSGGTIGMIALGSEDSERFYTAMGTIYLERLGEMASAALARVLS"
     gene            895..>1718
                     /locus_tag="PLL14_10815"
     CDS             895..>1718
                     /locus_tag="PLL14_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG04464.1"
                     /translation="MSTGARAASVSAYLDELAQQRRLSAHTVKNYGRDLDLLCGLIGE
                     LPGECGFGAIETHHIRRFVAQLHVRGLGGRSLGRTLSAWRGFYRWLGQHAMATRNPVD
                     SVRPPKSPQALPKALSPDEANRLLAAAAEDLLEIRDQAIFELFYSCGLRLAELAALDL
                     SCLDDLLAGEVRVLGKRNKLRLVPVGSKAREAVAVWAGQRARLAAADEAALFVGQRGR
                     RLGMRMIQLRLQRRGLAQGLPARVHPHMLRHSFASHVLQSSGDLRAVQEMLGHASIA"
BASE COUNT          272 a          572 c          592 g          282 t
ORIGIN      
        1 gaccggcatg ccatccgcct gcgggttttc gaacgtggtg ccggtgaaac cctggcttgc
       61 ggcaccggtg cctgcgctgc cgtggtcgcc ggcatcgccc gcggactgct cgattcgccg
      121 gtgcgcgtcg aaacgcgtgg cgggatgctg agcattgcct gggccgggaa gggcgcgccg
      181 gtgctgatga ccggccccgc ggttaccgta ttcaagggag agattgagtt atgagatcgg
      241 aagaagtcgc ccactatctg cgggacaatc cggggttctt cgaggaacac atcgatctga
      301 tcgccaacgt cgtggtcccg catccgcacg cggggcgaac gatctcgatc accgaacgcc
      361 agatgctggc gctgcgagac aagaaccggc aactcgaagg caagatgggc gagttgctgc
      421 agttcggcga ggaaaacgac gcgatcagcg tgaagatgca ccgtctggcg gtcggtttga
      481 tcgccgccag gagcttccag tcgctgctgc acacgctgaa ttttcacctg cgcgacgatt
      541 tcgccatccc gcacgtggcc gtgcgcctct ggcatcggcc cgaaggcagc gatgaactgc
      601 ccgaattcgc cgacgtcagc cgcgacctgc aggcttttgc cgaaacgctg gcgcagccct
      661 attgcggcac gaccagcggc ttcgagactt ccggctggtt cggcgaagcg gcgacgcacg
      721 tgcgttcgca agcgctgatc gcgatgcgca acagcggcgg cacgatcggc atgatcgcgc
      781 tcggcagcga ggacagcgag cgcttctaca ccgcgatggg taccatctac ctggagcgtc
      841 tcggcgaaat ggcgtcggcg gcgctggcgc gcgtcctgtc ctgaaaccag ggcgatgagc
      901 accggcgcgc gcgccgccag cgtcagcgct tacctcgacg aacttgccca gcagcggcgc
      961 ctgtcggcgc acaccgtcaa gaactacggg cgcgatctcg atctgctctg tggcctgatc
     1021 ggcgaactgc ccggcgaatg cggcttcggc gccatcgaga cgcaccacat ccgccgtttc
     1081 gtcgcccagc tgcacgtgcg tggcctgggc ggacgctcgc tggggcgtac gctttctgcc
     1141 tggcgcggtt tctatcgctg gctcggccag cacgcaatgg ccacgcgcaa cccggtagac
     1201 agcgtgcggc cgccgaaaag cccgcaggcg ctgccgaaag cgctgtcgcc ggacgaggcg
     1261 aatcggctgc tggcagcggc cgccgaagac ctcctggaaa ttcgcgacca ggcgatcttc
     1321 gaactgttct actcctgcgg cctgcgcctg gccgaactcg cggcgctcga cctgtcgtgt
     1381 ctcgacgacc tgctggccgg cgaggtgcgc gtgctcggca agcgcaacaa gttacggctc
     1441 gtcccggtcg gcagcaaggc ccgcgaagcg gtcgcggtgt gggccgggca gcgcgcccgc
     1501 ctggcggctg ccgacgaggc ggcgctgttt gtcggtcagc gcggccggcg cctggggatg
     1561 cgcatgatcc agttgcggct gcagcggcgc ggcctggcgc agggcctgcc tgcccgggtg
     1621 catccgcaca tgctgcgcca ctcgttcgcc tcgcacgtgc tgcagtcctc gggtgacctg
     1681 cgcgcggtgc aggagatgct cggacacgcc agcatcgc
//
LOCUS       DAOSIT010000542         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60447_length_1717_cov_6.615523, whole genome shotgun sequence.
ACCESSION   DAOSIT010000542 DAOSIT010000000
VERSION     DAOSIT010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60447_length_1717_cov_6.615523"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLL14_10820"
     CDS             <1..411
                     /locus_tag="PLL14_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG04465.1"
                     /translation="TEHLLMKDAEKNINMLKQLRHCGVGVSIDDFGTRYSSLNYLRRF
                     PINSIKIDQSFVRDLNPNNQSSTSIIHAIACIACSFGLRVIAEGVETETQRQLLSELD
                     CDDMQGFLFSRPLTAAQFEKFLSNSVFPPPDHRH"
     gene            427..>1717
                     /locus_tag="PLL14_10825"
     CDS             427..>1717
                     /locus_tag="PLL14_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04466.1"
                     /translation="MNSRCLARAAAALDDPIVLDWAVHYYSDLPPHSERREQLAAEWF
                     PGAALERWIEAGDFDTLSILLRELPEARWSALAPTMAAHWNEWPSQMASASAKILARV
                     APDLATRAFDQYLESPPPWSIDRVVAILDSLDRLPLPAALCLADKLRPAYDTQEQRLS
                     RYLSNHVFQATVALNQIDALPGLLDKLCAQNGAHLENAIEHMATCLFAHDAYADLFFM
                     RRKGYQDVSFHDLAALFEADTPLSEMDAILASDAPLPPALSLLETCHARTPGATLAWR
                     TIQSSDAYRNAQAPTQLAALALAAVADAFERKRIDVTRLPVDQVLQLLLLDVRTNIHS
                     APLVARLQDFPRDETVASIAQRLTDHRDSYGSVRLAEATGELACPEFTPGLIACLSKN
                     DGDFLCEAAQHALLTIGEPARDALIAQWERLDASQQIY"
BASE COUNT          336 a          606 c          473 g          302 t
ORIGIN      
        1 accgagcacc tgctgatgaa agacgccgaa aagaacatca acatgctcaa gcagctacga
       61 cactgcggcg taggggtgtc catcgacgac ttcggaaccc gctactcatc gctcaactac
      121 ctgcgccgtt tcccgatcaa cagcatcaag atcgaccagt ccttcgttcg cgacctcaac
      181 ccgaacaatc aaagctcgac ctcgatcatt cacgccatcg cctgcattgc ctgcagcttt
      241 ggcttgcggg tcatcgccga aggcgtcgaa accgaaaccc agcggcaatt gctcagcgaa
      301 ctggattgcg acgacatgca gggcttcctg ttcagccgac cattgaccgc tgcgcaattc
      361 gagaaattcc tctccaacag cgtcttcccg ccgcccgatc atcgccatta gaacaaccct
      421 gcgcacatga actcccgctg cctggcacgt gccgccgccg cactcgatga tccgatcgtg
      481 ctcgattggg ccgtccacta ctacagcgac ctgccgcccc actcggaaag gcgcgagcag
      541 cttgccgccg aatggtttcc cggcgccgcc ctcgaacgct ggatcgaagc cggtgacttc
      601 gacaccctca gtattctcct gcgggagctt cccgaagccc gctggtcggc cctcgccccg
      661 acgatggccg cacactggaa cgagtggccg tcgcagatgg ccagtgcgag cgcgaaaatc
      721 ctggcccgcg tcgcaccgga cctggcaacc cgggcctttg accaatacct ggagagtcca
      781 ccgccctggt cgatcgaccg cgtggtcgcc atcctggaca gcctggatcg cttgcccttg
      841 ccggcagccc tgtgcctggc cgacaaacta cggccggcgt acgacacgca ggagcagcgg
      901 ctgtctcgct atttgagtaa tcatgttttc caggccaccg tcgcactcaa tcagatcgac
      961 gccctgcccg gtctacttga caagctgtgc gcacagaacg gtgcgcatct ggaaaacgcc
     1021 atcgagcaca tggccacctg tttgtttgcg cacgacgcgt acgccgatct cttcttcatg
     1081 cgccgcaagg gctatcagga cgtatccttc cacgatctgg cggccttgtt cgaggccgat
     1141 acgccgctgt ccgagatgga cgccatcctc gcgtcggacg cgccactgcc gccggcgctg
     1201 agcctgctgg aaacgtgcca cgcccgcacg ccaggcgcga cgctggcctg gcgcacgatc
     1261 cagagcagcg acgcctaccg caacgcacaa gcgcccacgc aactggcagc gctggctctc
     1321 gcggccgtag cggacgcttt cgaacgcaag cgcatcgacg tgacgagact gcctgtcgac
     1381 caggtgctgc aattgctgct gctcgatgtg cgcaccaaca tccactcggc acccctggtc
     1441 gcccgtttgc aggacttccc cagggacgaa accgtggcca gcattgcgca acggcttacc
     1501 gaccaccgcg acagctacgg gtcggttcgc ctggctgaag cgaccggaga actcgcgtgt
     1561 cccgaattca ccccggggct gattgcctgc ctgtccaaaa atgatggcga ttttctgtgc
     1621 gaagccgcgc agcatgcact gctcacgatc ggcgaacccg cgcgcgatgc gctgatcgcg
     1681 caatgggaac ggctcgatgc ctcgcagcag atctacg
//
LOCUS       DAOSIT010000543         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60450_length_1717_cov_5.720217, whole genome shotgun sequence.
ACCESSION   DAOSIT010000543 DAOSIT010000000
VERSION     DAOSIT010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60450_length_1717_cov_5.720217"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLL14_10830"
     CDS             <1..255
                     /locus_tag="PLL14_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOG04467.1"
                     /translation="VSNRSVDGSISLLDTQTMSVVEKYEVPGGPDDMMVRADGKELWA
                     TARFARKVQVIDLVTKKLKMSIPVGSSPHGVFFLNHAGLR"
     gene            383..1090
                     /locus_tag="PLL14_10835"
     CDS             383..1090
                     /locus_tag="PLL14_10835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG04468.1"
                     /translation="MLLLTTPAAHAAAACQAGTLYLTFDTGNMRHAELIADTLARHGV
                     KATFFLAQEETARGDHALDASWAPYWRARVAEGHAFGSHTWRHGSFRSDSGERVRYRL
                     QDGRSETLDARAVCEELQRPGSRFQELTGHRLDPLWRAPGGRTTPNTLAAARACGYQH
                     VGWAPAGFLGDELPSDSYPNALLVQRALDRLKDGDIVMAHLGIWSRKDPFAPMLDALI
                     SGLKSKGFCFATRRPQP"
     gene            1087..>1717
                     /locus_tag="PLL14_10840"
     CDS             1087..>1717
                     /locus_tag="PLL14_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOG04469.1"
                     /translation="MSNTFLESFVALQGWLLAHLLEPVLLTLGLGSYLEMAFDGVEFF
                     LCGVLQITAAYLLLRPLEALRPIEVWPDRKAVRVDVLYSLLDRLGIIPLLVFALLAPL
                     FVSVDGWLRFHDIIPAQLEDLFPALEARPLLSFLIYLILLDFAEYWRHRLSHTLRWWW
                     ALHAIHHSQRQMTLWTDSRNHLLDDLIGGFWFAVIALLIGVPPGHFIILL"
BASE COUNT          274 a          578 c          538 g          327 t
ORIGIN      
        1 gtttcgaacc gctcggtcga tggcagtatt tcgctgctcg atacgcagac catgagcgtc
       61 gtggaaaaat acgaggtccc gggtgggccg gacgacatga tggttcgcgc cgacggcaag
      121 gaactctggg caacggcgcg cttcgcccgc aaggtgcagg tcatcgacct ggtcaccaag
      181 aagctcaaga tgtcgatccc ggtcggcagt tcgccgcatg gcgtcttctt tctcaaccac
      241 gcgggtctgc gatgaacgct tcaaggcatg gctggcctgg tccaacgggt gaacacgtgg
      301 catgaacgtc acagcaggtg tggccggaat aaccatgcac actagcctcc tggcccggat
      361 cctgacgctg ctgggcagcg gcatgctgtt gctgaccacg cccgcggcgc acgccgcggc
      421 ggcctgccag gcgggcaccc tgtacctgac gtttgacacc ggcaacatgc gccacgccga
      481 attgattgcc gatacgctcg ccaggcacgg cgtcaaggcg accttcttcc tggcgcagga
      541 agagacggca cgcggcgacc atgcgctcga cgccagttgg gcgccttact ggcgcgcgcg
      601 cgtcgccgaa gggcacgcct tcggcagcca cacctggcgg cacggcagct ttcgcagcga
      661 cagcggcgag cgggtccgct accggctgca ggatggccgc agcgaaacgc tggacgcgcg
      721 tgccgtgtgc gaggaattgc agcgtccggg cagccgtttc caggaactca ccggacatcg
      781 gctcgacccc ctgtggcgcg cgccgggtgg gcgcaccacc ccgaacacgc tcgcagcggc
      841 gcgggcctgt ggctaccagc atgtcggctg ggcaccggcc ggcttcctcg gtgacgagtt
      901 gccttccgac agctacccga acgcattact cgtgcagcgc gcgctcgacc gcctgaagga
      961 tggcgacatc gtgatggccc acctcgggat ctggtcgcgc aaggatccct ttgcgccgat
     1021 gctcgacgcc ttgatcagcg gcctcaagag caagggcttc tgttttgcca cccgccggcc
     1081 gcaaccatga gcaacacctt cctggaatcc ttcgtcgcgc tgcagggctg gctgctggcg
     1141 cacctgctgg agcccgtcct gctgaccctg ggactgggaa gctatctgga aatggccttc
     1201 gacggtgttg agttcttcct ctgcggggtg ctgcagatca ctgccgccta tctgctgttg
     1261 cggccactgg aggcgctgcg cccgatcgaa gtctggcccg accgcaaggc ggtgcgtgtc
     1321 gacgtgttgt attcgttgct ggaccggctg ggcatcatcc cgctcctcgt gtttgccctg
     1381 ttggcaccgc tcttcgtgtc ggtggatggc tggctgcgct ttcatgacat cattcctgcg
     1441 caactggaag acctgtttcc ggcgctggaa gcccgccccc tgctcagctt cctgatctat
     1501 ctgatcctcc tggacttcgc cgaatactgg cgccaccgcc tttcgcacac gctccgctgg
     1561 tggtgggcac tgcacgcgat tcaccacagc caacgccaga tgaccctgtg gaccgacagc
     1621 cgcaaccatc tgctcgacga cctcatcggc ggcttctggt ttgccgtcat cgcgctcctg
     1681 atcggcgttc cgccgggcca tttcatcatc ctgctga
//
LOCUS       DAOSIT010000544         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_60500_length_1716_cov_7.655629, whole genome shotgun sequence.
ACCESSION   DAOSIT010000544 DAOSIT010000000
VERSION     DAOSIT010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60500_length_1716_cov_7.655629"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PLL14_10845"
     CDS             complement(<1..1496)
                     /locus_tag="PLL14_10845"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOG04470.1"
                     /translation="MENEALWRPSADAVETAQLSSFIAAVNLRWQAGCSDYASLWRWS
                     VARPEAFWRSLWDECRVLGESGASVVEDGDRMPGARWFPQARVNYAENLLRRRDDADA
                     LVFWGEDKIRRRLSRGELYVQVSRCAQALKGAGVVQGDRVAAYLPNMPEALIAMLATA
                     ALGAIWSSASPDFGVQGVLDRFGQIEPKILLCVDGYWYNGKRVDCMAKNVEVAAQMPS
                     VERTLVVSCLDDSPVVSGIRNGVRYVDFVAPYAAAEMEFVPLPFAHPLFIMFSSGTTG
                     VPKCIVHCHGGALLQHLKEHQLHGDIRPDDRIFYFTTCGWMMWNWLVSALASGAALLL
                     YDGSPFVRGAGRPGTILFDYADAERMTHFGTSAKFIDAIAKSGLQPRQTHRLDHLRVM
                     FSTGSPLAPESFDYVYREIKADLLLASISGGTDILSCFALGSPILPVYRGELQCRGLG
                     MAVDVFDAEGRPIPAGLGERGELVCTAPFPAMPVGFWNDPDGAKYHAA"
     gene            complement(1516..>1716)
                     /locus_tag="PLL14_10850"
     CDS             complement(1516..>1716)
                     /locus_tag="PLL14_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOG04471.1"
                     /translation="LNYPGVVEVRMFCGRPGRSSVPTHYEIRLQGDDALYATGDSKIV
                     WMDVATGKSVPIPDELRMELPA"
BASE COUNT          350 a          547 c          531 g          288 t
ORIGIN      
        1 taggcggcgt ggtacttggc gccgtcggga tcgttccaga aaccgaccgg catcgccggg
       61 aagggcgcgg tgcataccag ttcgccgcgt tcgcccagcc ccgcgggaat cggccggccc
      121 tccgcgtcga agacatcgac ggccatgccg aggccgcgac attgcaactc gccgcggtaa
      181 accggaagga tcggactacc aagcgcgaaa cacgacagga tgtcggtacc gccagaaatc
      241 gaagccagca gaagatctgc cttgatttcg cgatagacgt agtcaaagct ctccggcgcc
      301 agtgggctgc cggtcgaaaa catgacgcga agatgatcga ggcggtgtgt ctgacgcggt
      361 tggaggccgc ttttggcaat cgcgtcgatg aacttggccg aagtcccaaa gtgtgtcatg
      421 cgctcggcgt cggcgtagtc gaaaaggatc gttcccggtc taccggcccc ccgcacgaac
      481 ggtgaaccat cgtaaagcag cagcgcggcg cctgaggcaa gcgccgaaac cagccagttc
      541 cacatcatcc agccacaggt cgtgaagtag aagatgcggt catccggtct gatgtcgccg
      601 tgcagttggt gctccttgag gtgctgcaac aaggctccac catgacagtg gacgatgcac
      661 ttcggcactc cggtggtgcc cgaggaaaac atgatgaata gcgggtgcgc gaaaggcagc
      721 ggcacaaact ccatctcggc ggcggcgtac ggcgcgacga agtcgacgta gcgcacgcca
      781 ttccggatgc cggacactac tggcgaatcg tccaggcagg agacgaccag cgtccgctca
      841 acgcttggca tttgcgcagc gacttcgacg ttcttggcca tgcagtcgac ccgcttgccg
      901 ttgtaccagt agccgtcaac gcacagcagg atcttcggtt caatctgccc gaaacggtcc
      961 aggaccccct gtaccccgaa atcaggcgat gcggacgacc agatggcgcc gagcgcagcc
     1021 gttgccagca tggcgatcaa cgcctcgggc atgttcggca gataggcggc gacgcggtca
     1081 ccctggacga caccagcacc cttcagcgcc tgcgcacagc gtgaaacctg cacgtaaagc
     1141 tcacctcggg agaggcggcg cctgatcttg tcctctcccc agaaaaccag ggcgtcagcg
     1201 tcatcgcggc ggcgcaggag gttctctgca taattgaccc gggcctgcgg aaaccagcgg
     1261 gcgcccggca tgcggtcgcc gtcctcgacg acgctcgcac cggattcgcc gagcacccgg
     1321 cattcatccc acagcgaacg ccagaatgct tcgggccggg ccactgacca gcgccagagg
     1381 ctggcgtaat ccgaacagcc ggcttgccaa cggaggttga ctgcggcgat gaaggaactg
     1441 agttgcgctg tctcgacagc atcggcgctg gggcgccaga gtgcttcgtt ttccatgtcc
     1501 ccgctccccc tgtgctcagg caggcaattc catacgcagt tcgtcgggaa ttggtaccga
     1561 cttgccggtc gctacatcca tccagacgat tttcgagtcg cctgtggcat acagagcgtc
     1621 gtcgccctgc aacctgattt cgtagtgcgt cggcacgcta ctgcgcccgg gacggccgca
     1681 gaacatgcgc acttcgacga cacccggata gttcag
//
LOCUS       DAOSIT010000545         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61075_length_1705_cov_7.972121, whole genome shotgun sequence.
ACCESSION   DAOSIT010000545 DAOSIT010000000
VERSION     DAOSIT010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61075_length_1705_cov_7.972121"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLL14_10855"
     CDS             <1..321
                     /locus_tag="PLL14_10855"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOG04472.1"
                     /translation="AASLMKRKLYAMAGIGDPPRFFRQLRTLGLDFEEHPFPDHHSYA
                     AGDLAFAQDGVLLMTEKDAVKCANLVTGEAWVLPVEAVIGTPPGHAGLFETILEKLHG
                     RTPA"
     gene            302..478
                     /locus_tag="PLL14_10860"
     CDS             302..478
                     /locus_tag="PLL14_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOG04473.1"
                     /translation="MDARLLDILVCPVCKANLEYRKARAELVCKPCRLAFPIRDGIPI
                     MLEDEARQLPSEDR"
     gene            535..1299
                     /gene="kdsB"
                     /locus_tag="PLL14_10865"
     CDS             535..1299
                     /gene="kdsB"
                     /locus_tag="PLL14_10865"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOG04474.1"
                     /translation="MPFKVVIPARYGSTRLPAKPLLDLGGKPMVVRVAERALLSGAEE
                     VVVATDHLDVLAAAERHGLRALLTRTEHASGTDRLAEVVELSGWSDDTTVVNVQGDEP
                     LIDPGLIALTAARLATSGADIATVAHPVADAADFFNPHVVKVVLTASGDALYFSRAPI
                     PYARDHFAREAGGETLPPGMHAYRHVGLYAYRASFLRTYTGLAPSPLESLEALEQLRA
                     LWHGFRISVAIADHLPLAGVDTPEDARRLQEWFEKV"
     gene            1431..>1705
                     /locus_tag="PLL14_10870"
     CDS             1431..>1705
                     /locus_tag="PLL14_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOG04475.1"
                     /translation="MKLILLGAPGAGKGTQAQFIREKFSIPQISTGDMLRAAVKAGTA
                     LGLAAKQVMDAGGLVSDEIIIGLVSDRLQQDDCQAGYLFDGFPRTLP"
BASE COUNT          310 a          525 c          534 g          336 t
ORIGIN      
        1 gccgcctccc tgatgaaacg caaactgtac gcgatggccg ggatcggcga tccgccgcgc
       61 tttttccggc agttgcgaac cctgggactc gatttcgagg aacatccctt tccggatcac
      121 cattcgtatg ccgccggaga tctggctttt gcccaggacg gcgtgctgct gatgaccgaa
      181 aaagatgcgg taaaatgcgc gaatctggtc accggcgagg cctgggtgct gccggtcgag
      241 gcggtgatcg gcacaccgcc cggtcatgct ggtctgttcg agacaattct ggagaaactc
      301 catggacgca cgcctgcttg atattttagt ctgcccggtc tgcaaggcca acctggagta
      361 ccgcaaggca cgcgccgaac tggtgtgcaa accgtgccgc ctggcttttc cgattcgtga
      421 cggcatcccg atcatgctcg aagacgaggc gcgccagttg ccgagcgaag atcgttgaat
      481 acctcggatg gttccagtga cgaagtcgcg gagtgcgcaa gggccggagc gcacctgccg
      541 ttcaaggtgg tcattcccgc ccgttacgga tcgacgcgcc tgccggccaa gcccttgctg
      601 gatctcggcg gcaagccgat ggtcgtgcgc gtggccgagc gcgcgctgtt gtcgggagcc
      661 gaggaggtgg tggtggccac cgaccacctg gacgtactgg ccgctgccga gcggcacggc
      721 ctgcgggcct tgctgacgcg taccgaacac gccagcggca ccgatcgtct cgccgaggtg
      781 gtcgagttgt cgggctggag cgacgatacg acggtagtga acgtgcaggg tgacgaaccc
      841 ctgatcgatc ctggattgat tgccctgacc gcggcccgtc tggcgacaag cggcgccgat
      901 atcgccacgg tggcccatcc tgtagccgat gcggctgatt tcttcaaccc acatgtcgtc
      961 aaggtggtgc tcacggcgag cggcgacgcg ctgtatttct cgcgcgcacc gattccctat
     1021 gcgcgcgacc actttgcgcg cgaggccggt ggcgaaaccc tgcctcccgg gatgcacgcc
     1081 taccggcatg tcggcctgta tgcctatcgt gcgagcttcc tgcgcaccta caccggcctg
     1141 gcgccgtcac cgctcgaatc gctggaggca ctcgaacaat tgcgcgcctt gtggcatggc
     1201 ttccgcatca gcgtggcgat tgccgatcac ctgccactcg ccggtgtaga caccccggag
     1261 gacgcccgtc gactgcagga atggttcgaa aaggtttgac cgtgcgccca tttgagggta
     1321 agtttccgcc tgttgaccac gacaacgtga tgctgcggtc ccgtaattga gatacaagta
     1381 tctgaaatac cggacaaact gtttcatttg attttctcaa aaggcgaacc atgaaactca
     1441 tcctgctagg cgcgccaggt gccggcaagg gcacccaagc ccagttcatt cgtgaaaaat
     1501 tcagcatccc gcagatctcc accggtgaca tgctgcgcgc ggcagtgaaa gccggtactg
     1561 ctcttggcct ggcagcgaag caggtcatgg acgccggcgg cctggtgtcg gacgagatca
     1621 tcatcggtct ggtcagcgac cgcctgcagc aggatgattg ccaggcgggt tatctgtttg
     1681 acggttttcc gcgcactctg ccgca
//
LOCUS       DAOSIT010000546         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61174_length_1703_cov_9.740898, whole genome shotgun sequence.
ACCESSION   DAOSIT010000546 DAOSIT010000000
VERSION     DAOSIT010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61174_length_1703_cov_9.740898"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PLL14_10875"
     CDS             <1..927
                     /locus_tag="PLL14_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOG04476.1"
                     /translation="HYLIVDGKLQIVDEYTGRVMADRSWERGLQQMIEVKEGLALSKR
                     RDTISRITYQRFFRRYLCLAGMTGTAIEVAPEMCSVYALEVLPIPPHRPLRRCDLGQR
                     VFLDSERRWAAVVERAAAMRADGRAVLIGTRSVAASELASQRLLAAGLPHAVLNARQD
                     AGEAAIIAQAGQPGRITVATNMAGRGTDIVLSTAVRQAGGLHVILSEYHDAARIDRQL
                     FGRAGRQGDPGSCEAIVSLDDELFVAHAALLLGWLRARVTPDGLLPAALGIWLRRFAQ
                     RAAEQQNSRIRRQTVERDKQLERSLAFAGRGE"
     gene            931..>1703
                     /locus_tag="PLL14_10880"
     CDS             931..>1703
                     /locus_tag="PLL14_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG04477.1"
                     /translation="MARGRGWLLLALLCGGASAGEFDCIIEPSRTIEVRAASEGLIEK
                     IWVDRGDSVKAGQVLVTLDSGVERAATEAARYRSTMQGRIRTGESRVEFSTAKYNRRE
                     KLAGQSFISLHDRDEALTEKRLAESELIEAKDDRRLAEIEFRRLSEQLRLRTIRSPVD
                     GVVVDRLLNAGELADNRDLRKPILRLADIGTLFVEVLMPIEAFGKLVLGQSAEVLPEA
                     PVGGRYTATVKVIDRVHDAASGTFGVRLEMANPGMKLPGG"
BASE COUNT          280 a          546 c          618 g          259 t
ORIGIN      
        1 cactacctga ttgtcgacgg caagctgcag atcgtcgatg agtacaccgg ccgggtgatg
       61 gccgatcgct cgtgggagcg cggcctgcag cagatgatcg aggtcaagga aggactggcc
      121 ctcagcaagc ggcgcgatac catttcgcgc atcacctatc agcgcttctt ccgccgctac
      181 ctgtgcctgg ccggcatgac cggcacggcc atcgaagtgg cgccggaaat gtgctcggtc
      241 tatgcgctgg aggtgctgcc gattccgccg catcggccac tgcggcgctg cgacctcggg
      301 cagcgcgtct tcctcgacag cgagcggcgc tgggcggcgg tggtcgagcg cgcggccgcg
      361 atgcgtgccg acgggcgcgc ggtcctgatc ggtacccgct cggtagcggc gtccgagctg
      421 gccagtcagc ggctgctcgc cgcaggcttg ccgcacgcgg tgctcaatgc gcgccaggac
      481 gcgggcgaag ccgcgatcat cgcgcaggcc gggcagccgg gccggatcac cgtcgccacc
      541 aacatggccg gccgtggcac cgacatcgtg ctcagcacgg cagtgcggca ggccggcggt
      601 ctgcacgtga tcctctcgga ataccacgac gcggcccgca tcgaccgcca gctctttggc
      661 cgcgccggcc gtcaggggga tcccggatcg tgcgaagcga tcgtctcgct cgacgacgaa
      721 ttgtttgtcg cgcacgctgc gctgctgctc ggctggctgc gcgcgcgcgt cacgcccgac
      781 gggctgctgc cggcagcgct cggaatctgg ctgcggcgat tcgcgcagcg cgcggccgag
      841 caacagaatt cacggattcg gcgacagaca gtggaacggg acaaacagct cgaaagatct
      901 ctggcgtttg ccggaagggg ggaatgaggg atggcgaggg ggagaggctg gttgctgctg
      961 gcgctgctct gcggcggagc gtccgccgga gagttcgact gcatcatcga accgagcagg
     1021 acgatcgagg tgcgcgcggc cagcgagggt ctgatcgaga agatctgggt ggaccggggc
     1081 gatagcgtca aagccgggca ggtgctggtg acgctcgatt cgggagtcga gcgcgcggcg
     1141 accgaagccg cgcgttaccg ttcgacgatg cagggccgga tcaggaccgg cgagagccgg
     1201 gtcgagttct cgaccgccaa gtacaaccgg cgcgaaaagc tggccggaca gtcgttcatc
     1261 tcgctgcacg accgcgacga ggcgctgacc gaaaagcggc tcgccgaatc cgaactgatc
     1321 gaggccaagg atgaccgccg cctcgcggag atcgagttcc gtcgcctgtc cgagcagttg
     1381 cgtctgcgca cgatcaggag cccggtcgat ggcgtcgtcg tcgaccgcct gctcaacgcc
     1441 ggggaactcg ccgacaaccg cgacctgcgc aaaccgatcc tgcggctggc cgacatcggc
     1501 acgctgttcg tcgaggtgct gatgccgatc gaggccttcg gcaagctggt tctcggccag
     1561 agcgccgagg tgttgcccga ggcgccggtc ggtggccgct acacggcgac ggtgaaggtc
     1621 atcgaccggg tgcacgacgc cgccagtggc actttcggcg ttcgcctcga aatggccaat
     1681 ccggggatga agctgccggg cgg
//
LOCUS       DAOSIT010000547         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61224_length_1702_cov_13.330298, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000547 DAOSIT010000000
VERSION     DAOSIT010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61224_length_1702_cov_13.330298"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLL14_10885"
     CDS             <1..423
                     /locus_tag="PLL14_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HOG04478.1"
                     /translation="SRLFDAGVDATCEAARRALLSQGYLLTTVRPDAIAASKHFQPDG
                     EVHVQIAFNVVCAPEGSQGRLATAYVSAIQDRYALKKSSNSASVGVSAFGSLSIPLTP
                     SEDSLVKVASETIPAGPFYDRFFALMQRLLAQQVDEQQ"
     gene            521..>1702
                     /locus_tag="PLL14_10890"
     CDS             521..>1702
                     /locus_tag="PLL14_10890"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOG04479.1"
                     /translation="MFKALLSLFRPRAASKPAEARPAAVAAADAGQTYLCHRPILDRG
                     QRVVAHELSVDVHRALRACPWQGPSRKLFEDVLLRQFAQARLEALLAQRRVFLPVGSG
                     ILEHPALHSLPRQNVVLQIAWHKEGRSGSPGTLADGMQRLRAMGFALACAHELLDGDE
                     KAGLLADYITVDVGRMAPPELLDVQRRLATHRQQQPLLAMNIAFMEMYQACRQIRFTH
                     FHGDFIRCSEEAPVDIDLPPYKAAAMELLNAIRAQASAAVLAERAWIDPTLVLRLLRV
                     VNSPAMRLRTPISDLKQGIAYLGYDELYRWVTLLLFSSQRADDRHPMEYAWRETALVR
                     GRFMELAAAGRLPAKACSQLFVVGVLSMIDKLFGMPMPYALEKFALSPEVSQALLFRR
                     GS"
BASE COUNT          266 a          581 c          573 g          282 t
ORIGIN      
        1 tcgcgcctgt tcgacgccgg cgtcgatgcg acctgcgagg ccgcgcggcg cgcgctgctc
       61 agccagggtt acctgctgac cacggtcagg cccgacgcca tcgccgccag caagcatttt
      121 cagcccgacg gcgaggtgca tgtccagatc gcgttcaacg tcgtatgcgc cccggagggc
      181 agtcagggca ggctggccac ggcctatgtc agcgccattc aggaccgcta cgcgctcaag
      241 aagagttcga attcggccag cgtcggcgtc agcgcgttcg gctcgttgtc gatcccgctg
      301 acgccgagcg aggattcgct ggtcaaggtg gccagcgaaa cgattcccgc cgggcccttc
      361 tacgaccgct tcttcgcgct gatgcagcgc ctcctcgcgc agcaggtgga cgagcagcag
      421 tagcccacaa gccgttgccg gccgtgccgc gcgggtcgag cggccggcgt ggacgcgtcc
      481 ccgccggcga cgagcacgta gcaccaacaa gaggaaaccg atgttcaaag ccctgttgtc
      541 ccttttccgc cccagggcag cgagcaagcc ggccgaggcg cggccggcgg cggtcgcggc
      601 ggcggacgcc ggccagacgt acctgtgcca ccggccgata ctcgaccgcg ggcaacgtgt
      661 ggtcgcgcac gagttgagcg tcgacgtgca ccgtgccttg cgtgcctgcc cgtggcaagg
      721 tccctcgcgc aagctgttcg aggatgtcct gttgcggcag ttcgcgcagg ccaggctgga
      781 ggcgctgctg gcgcaacgtc gggtattcct gccggtgggg agcggcattc tcgaacatcc
      841 ggcgctgcac agcctgccgc ggcagaacgt cgtgttgcag attgcctggc acaaggaagg
      901 ccgttccgga tcgccgggta cgctggccga cggcatgcag cgcctgcgcg ccatgggctt
      961 cgcgctggcc tgtgcccacg aactgctcga cggcgacgaa aaggccgggc tgctggcgga
     1021 ctacatcacc gtggacgtcg gccgcatggc gccgcctgaa ctcctcgacg tgcagcggcg
     1081 gctggccacc catcgccagc aacagccgtt gctggcgatg aacatcgctt tcatggagat
     1141 gtaccaggcg tgccggcaaa tccgcttcac gcatttccac ggcgatttca tccgctgcag
     1201 cgaggaggcg ccggtagaca tcgatctgcc tccctacaag gcggcggcga tggaattgct
     1261 caacgccatc cgtgcgcagg ccagcgctgc cgtgctggcg gaaagggcct ggatcgaccc
     1321 gacgctggtc ctgcgcctgc tgcgcgtcgt caactcgccg gcgatgcgcc tgcgcacgcc
     1381 gatcagcgac ctcaagcagg gcatcgccta ccttggctac gacgaactct accgctgggt
     1441 gacgctgctg ctcttttcca gccagcgcgc cgatgaccgg cacccgatgg aatacgcttg
     1501 gcgcgaaacc gcactggtcc gcggtcgctt catggaactg gccgccgccg ggcggctgcc
     1561 ggcgaaggcg tgcagccagc ttttcgtcgt cggcgtgctg tcgatgatcg acaagctctt
     1621 tggcatgccc atgccgtatg cgctggagaa gttcgccctg tcgccggagg tgagccaggc
     1681 gctgctcttt cgccgcggca gc
//
LOCUS       DAOSIT010000548         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61392_length_1699_cov_12.571168, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000548 DAOSIT010000000
VERSION     DAOSIT010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61392_length_1699_cov_12.571168"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1661
                     /gene="sulP"
                     /locus_tag="PLL14_10895"
     CDS             21..1661
                     /gene="sulP"
                     /locus_tag="PLL14_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOG04480.1"
                     /translation="MTWLRTYSRQDLRKDCIAGLIVAVMLVPQAMAYAMLAGLPPVVG
                     LYASTVPLLAYACLGTSRQLAVGPVAIVSLLVADACVGPAGTVADAATITGRAAALAL
                     LVGAIQIALGLCRAGFLLNFVSHAVVSGFTSAGAIMIGLSQFGHLLGVKLSGGNSVLA
                     LALDVLRNLSDTHLPTLAIGLGAVAIQLLARFKRWRFPVTLPIIAAAILLVWAFNLEA
                     YGVRAVGKVSSGLPAWRSPDMSWNGIRHLLPSAWMIVFVGTMESIAVAKWIATREKYR
                     IDANREMLGLGLANVAGGLFSGYPVAGGFSRTAVNYQAGARTPLASVFTAALVLLTLL
                     FLTPLFTHLPNAVLAAIILVAVIGLIDFKEARYLFKIKQADGWSFVVTFLVTLGFGMD
                     KGLLAGIAFSLMLFVLRSARPGMSILGRVGEQEFRDLRRYPDAATQSWLLILRFEASL
                     YFANAGFVEDRIRELLAQQPEVRWVLLDMSGVNDIDGVAVSMLTELMRDCAAANVQFA
                     CAGMKAGVRDIVRRAGWGDLPGDRMFYPNLQQALAALP"
BASE COUNT          264 a          489 c          553 g          393 t
ORIGIN      
        1 aaccgctgag gaaccccgcc gtgacctggt tgcggaccta cagtcggcag gacttacgga
       61 aagactgcat cgccggactg attgttgcgg tgatgttagt gccgcaagcg atggcctatg
      121 cgatgctggc cgggttgccg cctgtggttg ggctgtatgc ctcaaccgtg cccttgctcg
      181 cttatgcgtg cttgggtacg tcgcggcaac ttgcggtcgg gcctgtcgcg atcgtgtcgt
      241 tgttggtggc cgatgcctgc gtcggaccgg cgggtaccgt tgccgatgcg gcaacgataa
      301 ccggtcgggc ggccgcgctg gcgttgctgg tcggggcgat acaaatcgca ctcgggctgt
      361 gccgtgcggg ttttctgctc aacttcgtct cgcatgccgt ggtcagcggc tttacctccg
      421 caggcgccat tatgatcggc ctcagccagt tcgggcacct gctgggggtc aagttgtcgg
      481 gcggaaactc cgtgctggcg ctggcgctgg acgtgctgcg caacctttca gacacgcatc
      541 tgccgacact tgcgattggt ttgggcgctg tcgcgattca gcttctggct cgcttcaaac
      601 gctggcgctt cccggtcacc ctgccgatta tcgcagcagc catcctgctg gtctgggcgt
      661 tcaatctgga agcgtacggc gtgcgcgcgg tgggaaaggt ttccagcggc ctgccggctt
      721 ggcgttcgcc cgacatgtct tggaatggca tccggcacct gctgccttcg gcatggatga
      781 ttgtctttgt cggcacgatg gagtccatcg cggtggcgaa gtggatcgcc acccgtgaga
      841 aataccgcat cgacgccaac cgtgaaatgc ttggactggg attggccaat gtggcaggcg
      901 gcctgttttc cggctacccg gtcgcaggcg gtttttcccg cactgcggtg aactatcagg
      961 ccggcgcacg cacaccgctg gcgtcggtct tcacggccgc gctagtcttg ttgacgctgc
     1021 tgttcttgac gccattgttc acccatctgc ccaatgcggt attggcggcg atcattctgg
     1081 tggcggtgat cggcttgatc gatttcaagg aagcgcggta cctctttaaa atcaagcagg
     1141 ccgacggctg gagcttcgtg gtgacctttc tggtgacact gggctttgga atggacaaag
     1201 gactgctagc cggtatcgct ttttcgctca tgctgttcgt cctgcgcagc gctcgcccgg
     1261 ggatgtcgat tctgggacgg gtcggagagc aagagtttcg cgacctccgg cgttatccgg
     1321 atgcggccac gcagtcctgg ctgttaatcc tgcgcttcga agccagcctc tatttcgcca
     1381 atgccggatt tgtggaagac cggatcaggg aacttctggc gcaacagccc gaggtgcgct
     1441 gggtactgct ggacatgtcc ggcgtcaatg atatcgatgg cgtggccgta tcgatgttga
     1501 cggagctgat gcgcgactgc gccgccgcca acgtgcagtt cgcttgcgcc ggcatgaaag
     1561 cgggcgtcag agatatcgtc cgaagagccg gttggggtga tctgccgggt gaccggatgt
     1621 tctacccgaa tctgcaacag gctttggccg cgctgccata acccctccgt ttttagcaga
     1681 cgaggctctt gcaaaaccc
//
LOCUS       DAOSIT010000549         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61681_length_1694_cov_7.197071, whole genome shotgun sequence.
ACCESSION   DAOSIT010000549 DAOSIT010000000
VERSION     DAOSIT010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61681_length_1694_cov_7.197071"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1300
                     /gene="murA"
                     /locus_tag="PLL14_10900"
     CDS             41..1300
                     /gene="murA"
                     /locus_tag="PLL14_10900"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG04481.1"
                     /translation="MDKLLIQGGTPLAGEIAISGAKNAALPILCAGLLSSEPLHLSNV
                     PRLRDISTMLRLIEQMGVRVTSEGADGVSLDSRSLDNPLAPYDMVKTMRASILVLGPL
                     LARHGEARVSLPGGCAIGARPVEQHIRGLQAMGAEIHVEHGYIHAKAGAGGLKGARFV
                     TDMVTVTGTENLMMAAVLAAGETVIENAAREPEVVDLANCLVAMGAQISGAGTDRIRI
                     RGVERLHGATHPIMADRIETGTYLCAAAATGGQVRLTRTSAAYLDAVVDKLLDAGCDI
                     VIERDAITLQAPARLKAVSVRTAPYPAFPTDMQAQFMAINCVAEGSAVIRETIFENRF
                     MHAVELIRLGAKIRIDASNAMVTGVERLQGATVMATDLRASASLVIAGLVAQGETLIE
                     RIYHLDRGYEGIEEKLGQLGAYVRRIH"
     gene            complement(1347..>1694)
                     /locus_tag="PLL14_10905"
     CDS             complement(1347..>1694)
                     /locus_tag="PLL14_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOG04482.1"
                     /translation="ILLGAQVDFALMNKYLVGGAAVVAVLMFIARPVTVFVCAGPDRR
                     AQWSIKELLFMCWTRETGVIPAALAGLLLGMKAPGAEMIASVTFIAVLMTILIQAPTT
                     AWLAGRLGLLVKR"
BASE COUNT          324 a          547 c          561 g          262 t
ORIGIN      
        1 tcgatgaaaa cccagacccc cgacgaatgg agcgctgcgc gtggataagt tgctgatcca
       61 gggcggcacg ccgctggcgg gagaaatcgc catttccggc gccaagaatg cggcgctgcc
      121 gatcctctgc gccggcctgc tttcgtcgga gccactgcat ctgagcaacg tgccgcgact
      181 ccgggatatc tcgacgatgc tgcgcctgat cgaacagatg ggcgtacggg tcaccagcga
      241 gggtgctgat ggcgtgtcac tggacagccg cagcctggac aacccgctcg ccccctacga
      301 tatggtcaag accatgcgcg cctcgatcct ggtgctcggt cccctgctgg cgcgtcatgg
      361 tgaagcgcgg gtgtccctgc cggggggctg cgcgatcggc gcgcgcccgg tggagcagca
      421 catccggggg ctgcaggcga tgggcgccga aatccatgtc gagcacggct acatccatgc
      481 caaggcgggc gccggcggcc tcaagggggc gcgcttcgtc accgacatgg tcaccgtcac
      541 cggcaccgag aacctgatga tggccgccgt gctcgcggcc ggagaaacag tcatcgagaa
      601 cgccgcacgc gagccggagg tcgtcgatct ggccaattgc ctggtggcca tgggcgcgca
      661 gatctccggt gccggcaccg acaggattcg catccgggga gtggagcgtc tgcacggggc
      721 gacacacccg atcatggccg accgcatcga gaccggcacc tatctttgcg ccgcggcagc
      781 aaccggcggc caagttcgcc tgacccggac gtcagcggcc tatctggatg cagtggtcga
      841 caagttgctc gacgccggtt gcgacatcgt catcgagcgc gatgcgatta ccctccaggc
      901 accggcgcga ctgaaagcgg tcagcgtgcg caccgcgccc tacccggcct ttccgaccga
      961 catgcaggca cagttcatgg ccatcaactg tgttgccgag ggatcggcgg tgattcgcga
     1021 aacgatcttc gagaatcgct tcatgcatgc cgtcgaactg atccgcctgg gggcaaagat
     1081 caggatcgac gccagcaacg cgatggtcac cggcgtcgaa cgtctccagg gggcgacggt
     1141 aatggccacc gacttgcgcg cctcggccag cctggtcatc gccggactgg tggcgcaggg
     1201 cgaaacgttg atcgagcgaa tctatcatct cgaccgcggt tacgaaggca tcgaggaaaa
     1261 gctgggacag ctcggagcgt acgtcagacg gattcactga accgtcaggg cgcgccccac
     1321 gcatcatggg gcgcgtcggc gaggcctcaa cgcttgacca gcaagccgag gcgtcccgcg
     1381 agccaggcgg tggtgggcgc ctgaatcagg atggtcatca gcacggcgat gaaggtcacg
     1441 gacgcgatca tctccgctcc gggagccttc atgccgagca gcaaaccggc cagcgcggcc
     1501 ggaatgacgc cggtttcgcg cgtccagcac atgaacagca gttccttgat gctccactgc
     1561 gcgcgtcggt cgggaccggc acagacaaaa acggtcaccg gtcgagcgat gaacatcagc
     1621 acggcgacga ccgctgcacc accgaccaga tatttgttca tcagggcgaa gtcgacctgc
     1681 gcgccgagca ggat
//
LOCUS       DAOSIT010000550         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_61792_length_1692_cov_7.994502, whole genome shotgun sequence.
ACCESSION   DAOSIT010000550 DAOSIT010000000
VERSION     DAOSIT010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61792_length_1692_cov_7.994502"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLL14_10910"
     CDS             complement(<1..995)
                     /locus_tag="PLL14_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG04483.1"
                     /translation="MSPLQNRVANPSLFCLIALALNAPAVAAPAVVRVDGSSTVYPIT
                     EAVAEEFQKARKGAVNVTVGISGTGGGFKKFCRGEIDIANASRPIFRKEMEECARAGI
                     RYYEMPVAYDALTVMVNPQNTFIHSMTVAELRKIWEPAAQGRISSWQDVNPSWPDQKI
                     KLYGAGSDSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGISGDRGALGYFGFAYY
                     IENRKKLKAVAIDGGKGPVSPTPESVENGSYQPLSRPIFIYVSEKSLDRPEIREFVDF
                     YMKNATHLVKEVKYIPLPTKAYTGNLEHLARKKLGTVFGGQNEVGLTIEQLMKREA"
     gene            complement(1191..1541)
                     /locus_tag="PLL14_10915"
     CDS             complement(1191..1541)
                     /locus_tag="PLL14_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04484.1"
                     /translation="MSLSAALPGVDVIDAAAHELKRRRFSGTASESPAQQASKTKQSQ
                     ALSRVRCNVRLTARELKMLARLKKRLARRGIATCKQDLVRAGLLLLVHLGRTDLKTAI
                     RDVIAPEPAPGEDH"
BASE COUNT          304 a          474 c          531 g          383 t
ORIGIN      
        1 gcttcgcgct tcatcaactg ctcgatggtc aggcccactt cgttctgtcc accaaagacg
       61 gtaccgagct tcttccttgc caggtgctca agattgccgg tgtaggcctt cgtcggcagc
      121 gggatgtact tcacctcctt gaccaggtgc gtggcgttct tcatgtagaa gtccacgaat
      181 tccctgattt ccggccgatc gagtgatttc tcgctgacgt agatgaagat gggtcttgaa
      241 aggggctggt agctgccgtt ctcgacgctt tccggtgtcg gtgaaaccgg gcccttgccg
      301 ccatcgatgg ctacagcctt gagcttcttc cggttttcga tgtagtaggc gaatccgaag
      361 taaccgagcg ctccccggtc acctgaaatg ccctgtacaa ggacattgtc gtcttccgag
      421 gccgtaaagt caccgcggct cgatttggcc ttgccgacga tggcttcggt aaagtagtcg
      481 aaggtgccgg aatcggaacc ggcaccataa agtttgatct tctggtcggg ccaggacgga
      541 ttgacgtcct gccagctgct gatcctgcct tgcgcggcgg gttcccagat cttcctgagt
      601 tcggcgacag tcatgctgtg gatgaaggta ttctgcgggt tgaccattac cgtcagcgcg
      661 tcataggcaa ccggcatctc gtagtaacgg atgccggccc tggcgcattc ctccatttcc
      721 tttcggaaga tcggacgcga ggcgttggcg atgtcgatct cgccgcgaca gaatttcttg
      781 aagccaccac cggttccgga gatgccgacg gtgacgttga ccgcgccctt cctggctttc
      841 tggaactcct cggcgaccgc ctcggtgatc ggatagacgg tactcgaccc gtccaccctg
      901 accactgccg gcgccgcgac cgccggcgcg ttcaaggcaa gggctatcaa acaaaagagt
      961 gacgggttgg cgacccggtt ctgcagagga ctcatgacta actccagtgg gatgttgatt
     1021 gcagcttcgg agttattgta tgcgggcgat gtgacggcga gatgacgcct ggtgtgtgcg
     1081 tgtcttgcac gtaaggattg gcggcatggc ctgcagatga gcggtcggca aggcggtgca
     1141 gacgccggga agcgtttgat cgatgttccg agttcgacag gcttgtatcg ctagtggtct
     1201 tcacccggtg ccggttccgg ggcgatgaca tcgcgaatcg ccgtcttgag gtcggtccgg
     1261 cccagatgca ccagcaacaa caggccggcg cgtacgagat cctgcttgca ggtagcaatt
     1321 ccccggcgtg cgagacgctt tttcagcctg gcaagcatct tcaactccct ggcggtgagt
     1381 cgcacgttac aacggacgcg cgacagcgct tggctttgct tagttttgct ggcttgctgt
     1441 gccggggatt cagaggccgt tccggaaaac cgccggcgtt tcagttcatg ggcggcagcg
     1501 tcaatgacgt caacgccggg aagggcggca gataatgaca tgataatctc catgggcgga
     1561 ttgagaaatc aggactgcgt gctgtggccg gtgaaggcca gcgggctccc gggcggtgcc
     1621 tgggcgtccc gctggtcacc atcatgatcc gtgcatcagg gcctcgagta acttgcgcat
     1681 gtcggtggga cg
//
LOCUS       DAOSIT010000551         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_62939_length_1671_cov_9.148515, whole genome shotgun sequence.
ACCESSION   DAOSIT010000551 DAOSIT010000000
VERSION     DAOSIT010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62939_length_1671_cov_9.148515"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLL14_10920"
     CDS             <1..839
                     /locus_tag="PLL14_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG04485.1"
                     /translation="YALLASPHFGIHLPFWVILPLGAGLACIFGVLLGAPTLKLRGDY
                     LAIVTLGFGEIVRIFMNNLNAPVNITNGAQGITLIDPIRIGDFSFAQTSSFLGVTLSG
                     PQKYYFLLVALALLVIMVNIRLQDSRIGRAWQAIREDEVAAKACGINTRNIKLLAFAM
                     GASFGGIAGGVFSAMQGFVSPESFSLMESITILAMVVLGGMGHIPGVILGAILLTGLP
                     EALRYGIEPLQRALFGRMIVDPESLRMLVFGLALVLVMRFRPAGLWPSSQRRRELAES
                     AG"
     gene            842..1618
                     /locus_tag="PLL14_10925"
     CDS             842..1618
                     /locus_tag="PLL14_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG04486.1"
                     /translation="MSEHQLLLEAHAVAKHFGGVKALHDVSLTIRRGEIYGLIGPNGA
                     GKTTFFNCLTGLYRNEGGHFIFDGVRLQADAPHKAAAVGIARTFQNIRLFGNMTALEN
                     VMVGRHLRTRAGVVGAIIRNPATRAEEAGIARRAEELLHYVGIHARAHDYAKHLSYGD
                     QRRLEIARALATEPKLLALDEPAAGMNGTETEALRVLVEGICRDGVTVLLIEHDVKLV
                     MGLCGRVAVLDYGEKICEDVPAVVQKDARVVEAYLGGSVA"
BASE COUNT          289 a          471 c          534 g          377 t
ORIGIN      
        1 cctacgcact cctggcgtcg ccgcatttcg gcattcacct gccgttctgg gtgatcctgc
       61 ccctaggcgc cggattggcg tgcatcttcg gcgtcttgct gggtgcgccg acactcaaat
      121 tgcgcggcga ctatctggcc atcgtgacgc tgggtttcgg tgagatagtc cgtattttca
      181 tgaacaatct gaatgcgccg gtcaacatta ccaacggtgc gcagggaatc accctgatcg
      241 atccgattcg gattggcgat ttttcctttg cccagaccag cagtttcctc ggggtgaccc
      301 tttccggacc gcagaagtac tatttcctgc tggttgccct cgccttgctg gtgataatgg
      361 tcaatatccg tttgcaggat tcgcgcatcg gtcgtgcctg gcaggccata cgcgaggacg
      421 aggttgctgc caaggcatgc gggatcaaca cccgaaacat caagttgctg gcctttgcca
      481 tgggggcttc tttcggcggt atcgccgggg gcgttttttc cgcgatgcag ggcttcgtca
      541 gtccagagag tttctcgctg atggaatcga taaccatcct ggcgatggtc gtacttggtg
      601 gcatggggca tattcccggc gtgatcctcg gcgccatcct gttgaccggc ctccccgagg
      661 ccctgcgtta tggcatcgag ccgttgcagc gagcactctt cgggcgcatg atcgttgatc
      721 ccgagagctt gcggatgctg gtcttcggac ttgcgctggt gctcgtcatg cgtttccgtc
      781 cggcgggtct gtggccctcg tcgcaacggc gccgcgaact tgccgagagt gcgggttgac
      841 gatgagcgaa catcagttgt tgcttgaggc gcacgcggtg gccaagcact tcggcggggt
      901 caaggccttg catgatgttt ccctgaccat acggcggggc gagatctatg ggctgatcgg
      961 cccgaacggg gcgggcaaga ccactttttt caattgtctt accggacttt accgaaacga
     1021 gggtggccac ttcatctttg acggggtcag actccaggcg gatgcgccgc acaaggccgc
     1081 agcagtcggg attgcccgta cgtttcagaa catccgcctg ttcgggaaca tgacggccct
     1141 cgaaaatgtc atggtcggcc gccacttgcg cacgcgcgcc ggcgttgtcg gcgcgatcat
     1201 tcgcaatccg gcgacgcgtg ccgaagaggc ggggattgct cgtcgcgcgg aagaactcct
     1261 gcactatgtc ggcattcacg cgcgcgcaca cgactatgca aaacatcttt cgtacggtga
     1321 ccagagacgt ctggagattg ctcgcgcact ggcgaccgaa cccaagcttc tggcgctcga
     1381 cgagccggca gccgggatga acggcaccga aaccgaagcc ttgcgcgtgc tggtcgaggg
     1441 tatctgtcgc gacggggtta cggtgttgct gatcgagcat gacgtcaaac tggtcatggg
     1501 tttgtgcgga agggtcgcag tgctcgatta tggcgagaag atctgcgagg acgtgccggc
     1561 tgtggtgcaa aaggatgcgc gtgtcgtcga ggcctacctc ggtggctcgg tagcctgaag
     1621 ggagcgatga atcatgctcg aagttaacgg actgcgcgtt tcctacggtg g
//
LOCUS       DAOSIT010000552         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_63473_length_1661_cov_13.740349, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000552 DAOSIT010000000
VERSION     DAOSIT010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63473_length_1661_cov_13.740349"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="tolB"
                     /locus_tag="PLL14_10930"
     CDS             complement(<1..955)
                     /gene="tolB"
                     /locus_tag="PLL14_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOG04487.1"
                     /translation="MLGIVIKLRRLGLLAIGALALLQASAHAQLTIEITGAGANRIPV
                     AIADFGGEASAARVVTSVVRGDLERSGLFRMIDPGAAVMTEASAPVYEEWKSRGADAL
                     AAGSIGESTDGRREARFRLYDITKQSVLGGSAFVTSKPMLRAAGHRIADMIYEKLTGE
                     PGIFSTRVAYVIKAGAARYELHISDADGQNAQVALISKEPIISPSWSPEGGRLAYVSF
                     ENKKPVVYVHSLASGKRIVVANFKGSNSAPAWSPDGRKLAVVLSKDGGSQIFTVNADG
                     SGVLRLTTANAINTEPNFSPDGQFVYFTSDRGGSPQIYRIAA"
     gene            complement(1167..>1661)
                     /locus_tag="PLL14_10935"
     CDS             complement(1167..>1661)
                     /locus_tag="PLL14_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOG04488.1"
                     /translation="KEASRKEDPRKPETDPRPNPRDALDRELKELKQQKAEQKAAEDR
                     RARDEAQERLRKQLQTEQASAEKARAQSLDEEAYKHRIRLKVRNNVSLPPGIQGNPKA
                     EFEVTQLPTGDVLDVRLLRSSGNPALDAAVERAIRKSSPLPKPAQPELFKRVLNLSYR
                     PQDD"
BASE COUNT          293 a          494 c          525 g          349 t
ORIGIN      
        1 cggcggcaat tcggtagatc tgcgggctgc cgccacggtc ggacgtgaaa tagacgaatt
       61 gtccgtcagg agaaaagttg ggttcggtat tgatcgcatt ggctgtggtc aggcgtagca
      121 cgccggatcc atcggcgttg accgtgaaga tctgcgagcc gccgtccttg ctcagcacca
      181 ccgccagctt gcgcccgtct ggcgaccacg ccggcgccga attagaaccc ttgaagttgg
      241 ccacgacgat acgttttccc gaggccagtg aatgcacata caccaccggc ttcttgtttt
      301 cgaaggacac ataggccaga cggccaccct cgggtgacca ggagggcgag ataatgggct
      361 ccttcgagat cagcgccacc tgtgcgttct ggccgtcggc gtcggagatg tgcaactcgt
      421 aacgcgcggc gccggccttg atcacataag cgactcgtgt ggaaaagatg ccgggttcac
      481 cggtcagctt ttcgtagatc atgtcggcga tgcgatggcc ggcggcacgc aacatcggct
      541 tagaggtcac gaaagcggag ccgccgagca cgctctgctt ggtgatgtcg tagagccgga
      601 aacgggcctc acgtcgaccg tcggtgcttt cgccgatgct gccggcagcc agcgcgtcgg
      661 caccccggct tttccactcc tcgtagaccg gggcggacgc ctcggtcatg accgctgcgc
      721 cagggtcgat catcctgaac aggccgctac gttccaggtc gccacgtacg acggacgtaa
      781 cgacgcgtgc tgcgctggcc tcgccgccaa aatcggcgat cgccaccggg attcggttgg
      841 cgccggcacc ggtgatttca atggtcagtt gggcgtgcgc cgaagcctgc agcagggcca
      901 gcgcgccgat ggccaggagg ccgagacgac gaagtttgat cacgattcca agcatggggc
      961 agtccagtac agaatgcgcg attatatcgt ctctgaatgc gcttgatttg tgacgaaacg
     1021 ttatcttgcc gaatgccgga cgctgccggc atgaactgac tcttgaagat cttgagggcg
     1081 agttcgcggg aatcggcgaa gcggtcgagt tgatgggcgt tcacgctggt gagcagaggc
     1141 catgattacg caaccgcggt gctttgctag tcgtcctggg gtctatagga caggttcaga
     1201 acacgcttga agagttcggg ttgggccggc ttgggcaacg gcgacgactt gcggatcgct
     1261 cgctcgaccg cagcatcgag cgccgggttc ccgctcgacc ggagtagcct gacatcgagc
     1321 acatcgccgg tgggaagctg cgtcacttcg aactccgcct tcgggttacc ctggatgccg
     1381 ggtggcaacg acacgttatt gcgaaccttg aggcgaattc tgtgcttgta cgcctcctcg
     1441 tcgagcgact gcgcacgtgc tttctcggcc gacgcctgct cggtctgcaa ctgcttgcgt
     1501 agacgctcct gagcttcatc gcgcgcgcgc ctatcctctg cggctttctg ctcagccttc
     1561 tgctgtttga gttccttcag ttcacgatcc agggcatcac gcgggtttgg ccttggatcc
     1621 gtttccggtt tacgcggatc ttccttccgg gacgcttcct t
//
LOCUS       DAOSIT010000553         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_63675_length_1657_cov_7.757803, whole genome shotgun sequence.
ACCESSION   DAOSIT010000553 DAOSIT010000000
VERSION     DAOSIT010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63675_length_1657_cov_7.757803"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLL14_10940"
     CDS             <1..206
                     /locus_tag="PLL14_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HOG04489.1"
                     /translation="HQIQLKRRLESIKRIHQATDTLEERIEELAHVEERLVLQVGALA
                     QIGRSLRDILQQTVSSEGALSAA"
     gene            379..>1657
                     /locus_tag="PLL14_10945"
     CDS             379..>1657
                     /locus_tag="PLL14_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HOG04490.1"
                     /translation="MTSTPARSCALFMALMLALSPAAAQQSPSTVSPASAGGPAVSTF
                     SQQELDRLLAPVALYPDPLLAQVFMASTYPLEVVEAARWVKANPKVTGKALEDAMARQ
                     SWDPAVKSLTTVPQVLQQMNENLDWTQKLGDAFLAQQQDVMNTVQGLRARADAAGNLK
                     SSPQQVVKTEKQGSQTIYVVESPKPDVVYVPTYNPAVVYGTWWYPTPPYVVYPPAYVY
                     PPGLAFATGVVVGAAIWGACSWGWGRSNVDINVNRYNSFNRTNISSSNWNHNVEHRRG
                     VAYRDQNVARQYDRGGNAQAAKAREDFRGRADAGRSELKGMDRGELDHRVKDADRGSR
                     ENLGGRGDAGRGEPRERDRGEVSNRMQNGERGAGAVGRDRADAGGVQNRATGTHDRSS
                     GGGFSGVGNGASTREASQRGSASRADAGNRGGGG"
BASE COUNT          321 a          487 c          567 g          282 t
ORIGIN      
        1 agcaccagat ccagctcaaa cgtcggctgg aaagcatcaa gcgcatccac caggcgaccg
       61 acacgctgga agagcgcatc gaggaactcg cccatgtcga ggagcgcctg gtcctgcagg
      121 tgggagcgct cgcccagatc ggccggagtc tgcgcgacat cctgcagcag acggtaagca
      181 gcgaaggcgc gctgagcgcc gcgtgaggag aggtaagccg ggggagctgc acaacccctc
      241 ggatccgtag cgtgggcggc tgtggattcc ggctccaagg cctgcttcac cagcattgac
      301 ccggaacctg gcggcaggag tcgattcctg gcagccaatt cattttgtga agctactttc
      361 tgtgttcgag gatgattgat gacatcaaca cctgcccgat cctgtgcgct gttcatggca
      421 ctcatgcttg ccttgtcgcc ggctgcggcg cagcaatccc catcgacggt gtcgcccgcg
      481 tccgccggcg gtccggcagt gagcaccttc tcgcagcagg aactcgaccg cctgctggct
      541 ccggttgcgc tgtatcccga tcccttgctg gcgcaggtgt tcatggcgtc gacttatccc
      601 ctcgaagtcg ttgaggcggc tcgctgggtc aaggccaatc ccaaggtcac cggaaaggca
      661 ctcgaggatg cgatggccag gcagtcgtgg gatcctgccg tcaagtcgct gaccaccgtg
      721 ccgcaggtgc tgcagcagat gaacgagaat cttgactgga cccagaagtt gggagacgcg
      781 ttcctcgcgc agcagcagga cgtgatgaac acggtgcagg gtctgcgtgc cagagccgat
      841 gctgccggca atctgaagtc ctcgccgcag caggtggtga agaccgaaaa acagggtagc
      901 cagacgatct acgtcgtcga atcgccaaag ccggacgtgg tgtatgtgcc gacctacaat
      961 ccggcggtgg tgtatgggac ctggtggtat ccgaccccac catatgtcgt gtacccgccg
     1021 gcctatgtct atcctcccgg cctcgcattc gcgaccggag ttgtcgtcgg tgcggcgatc
     1081 tggggtgcct gcagctgggg ttgggggcgc agcaacgttg acatcaatgt caatcgctac
     1141 aactcgttca accgcacgaa tatcagcagc agcaactgga atcacaatgt cgagcaccgt
     1201 cgtggggtgg cctatcgcga tcagaatgtt gcccgccaat atgaccgtgg cggcaatgcg
     1261 caggcggcca aagcacggga agatttccgt ggccgcgccg acgcgggacg ttcggaactc
     1321 aagggcatgg atcgtggcga actcgaccac cgcgtcaagg acgccgatcg cggatcacgg
     1381 gaaaacctgg gtggacgggg cgacgcgggc cggggcgagc ccagggaaag ggatcgcggt
     1441 gaagtgagca atcgcatgca gaatggcgaa cgtggcgccg gtgctgtggg gcgtgaccgc
     1501 gccgacgccg gcggcgtaca gaatcgtgcc accggaacgc acgaccgttc gtcgggtgga
     1561 ggtttctccg gggtgggcaa tggtgcgtcg acgcgtgagg caagccagcg cggtagtgcc
     1621 agccgggcgg atgcgggcaa tcggggaggc ggtggcg
//
LOCUS       DAOSIT010000554         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_63834_length_1654_cov_9.015009, whole genome shotgun sequence.
ACCESSION   DAOSIT010000554 DAOSIT010000000
VERSION     DAOSIT010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63834_length_1654_cov_9.015009"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..883
                     /locus_tag="PLL14_10950"
     CDS             212..883
                     /locus_tag="PLL14_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG04491.1"
                     /translation="MAPSRIAQTALYQEVAERLRQRIFAHELPPGIRIDEQLLTVDYG
                     ISRTPLREALKVLAAEGLVTLRPRRGCFVTEISEQDLDEIFPVMAMLEGRCALEATHR
                     AQAGEIAELEAIHRQLEYFANSNQIERFFEANQQFHLRIQEMSGNRWLRQAIQDLRKV
                     LKLTRLFSLSIEGRLQQSLAEHAAILAAIRAGDAIRAQTTMHDHILAGRQAVARSQPQ
                     QEAIA"
     gene            880..>1654
                     /locus_tag="PLL14_10955"
     CDS             880..>1654
                     /locus_tag="PLL14_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOG04492.1"
                     /translation="MSGRILCVREDAIATVTLSNPGKLNAIDFAMWQKLAEVLREVSG
                     DGQVRCVVLRGDGEQAFAAGGDIEEFLHRRDTLERALAYHAQVGAALQAVFDCRHPTI
                     ALLQGACIGGGLEIAAQCDLRVCGESSRFGAPINKLGFSMYPGEMEGLLRLAGAATVF
                     EILLEGRILGAAEALAKGLVNRVVADVEVVAEAYASARRICNGAPLVATWHKQWVRRL
                     QHDVPLSEQELRASFAFLDTEDYREGLSAFLEKRKPDFRG"
BASE COUNT          306 a          493 c          543 g          312 t
ORIGIN      
        1 gttcgcgttg gacatgacgg aacctttcaa gaaatctgtg gattgaggcg gtactcccgc
       61 ctgacgacgc agaggcctta ctgacccagg gtgccgcgcc gccgtttcgg tgattttaac
      121 gcttgacacc ccgccccgcg aataatactg tatccataat tatgaaagca aggtccctta
      181 gagaccctga acctcttagc gaaaaagatc gatggctccg agtcgcatcg cccagactgc
      241 cctctaccag gaagtcgctg aacgactacg gcagcgaatc ttcgcgcacg agttgccgcc
      301 gggaatccgg attgatgaac aactgctgac ggtcgattat ggcatcagcc ggacgccctt
      361 gcgggaagcc ctcaaggttc tggctgccga aggcctggtg accctgcggc cgcgtcgcgg
      421 ctgtttcgtg accgagatct cggagcagga tctggacgaa atcttcccgg tcatggccat
      481 gctcgaaggg cgctgcgcgc tggaagcgac gcaccgggcg caagccgggg aaatcgccga
      541 acttgaggcg attcatcggc aactcgagta cttcgccaac agcaaccaga tcgaacgttt
      601 tttcgaggcg aaccagcagt ttcacctgcg catccaggaa atgtccggca accgctggtt
      661 gcggcaggcg atccaggacc tgcgcaaggt actcaagctg acgcgtctgt tctccttgag
      721 catcgagggt cggttgcagc agtcgctggc ggaacacgcg gcgattctcg cggcgatcag
      781 ggccggcgac gcaatacgcg cgcagaccac gatgcacgat cacatcctgg cggggcgaca
      841 ggcggtggcc agaagccagc cgcagcagga ggcgatagcg tgagcgggcg catcctttgt
      901 gtccgcgagg acgccatcgc caccgtcacc ttgtccaatc cgggcaaact caacgccatc
      961 gattttgcga tgtggcagaa gctcgccgag gtattgcgcg aggtttcggg cgacgggcag
     1021 gtgcgctgtg tcgtcctgcg gggtgatggc gagcaggcgt ttgccgcagg gggcgacatt
     1081 gaggagttcc tgcatcggcg cgataccctg gagcgtgcgc tggcgtatca cgcgcaggtt
     1141 ggcgctgcct tgcaggcggt gttcgactgt cgccacccga cgatcgccct gctgcagggt
     1201 gcgtgcatcg gcggtggcct ggaaatcgcc gcgcaatgtg atctgcgcgt ctgcggcgag
     1261 tcgtcacgct tcggagcgcc gatcaacaag ctcggcttct cgatgtatcc cggcgagatg
     1321 gaaggtctgt tgcgtctggc cggggcggcg acggtgttcg aaatcctgct cgaaggccgc
     1381 attctcggcg ccgccgaggc cctggccaag ggcctggtga atcgtgtcgt cgccgatgtc
     1441 gaagtggttg ccgaggccta tgcgagtgcc cggcgcattt gcaatggggc gccgctggtc
     1501 gccacctggc acaagcaatg ggtgcggcgc ctgcagcacg atgtgccgct cagcgagcag
     1561 gaactgcgcg cctcgtttgc ctttctcgat accgaagact atcgcgaagg gctgtcggcg
     1621 ttcctcgaaa agcgcaagcc ggattttcgc ggtc
//
LOCUS       DAOSIT010000555         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_63895_length_1653_cov_12.299750, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000555 DAOSIT010000000
VERSION     DAOSIT010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63895_length_1653_cov_12.299750"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="rpsA"
                     /locus_tag="PLL14_10960"
     CDS             complement(<1..1253)
                     /gene="rpsA"
                     /locus_tag="PLL14_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOG04493.1"
                     /translation="MSANPSMQESFAELFEESLGRQEMRPGEVITAEVVRIDQNFVVV
                     NAGLKSESYIEIDEFLSDKGELEVKVGDFVQVAIEQLENGFGETRLSRDRAKRVAAWN
                     ALEQALNDGSLVTGTITGKVKGGLTVMTNSVRAFLPGSLVDMRPVKDTTPYEGKTYEF
                     KVIKLDRKRNNVVVSRRAVLEASVGEERDALLAALKEGSIVKGVVKNITDYGAFVDLG
                     GIDGLLHITDLAWRRVRHPSEVLAVGDEVQAKILKFDQEKNRVSLGLKQLGEDPWVGI
                     SRRYPPTTRLFGKVTNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEV
                     EVMILEIDEDRRRISLGMKQCAANPWDDFSMNYKKGDRVRGAIKSITDFGVFIGLPGG
                     IDGLVHLSDLSWSLPGEEAIRNFKKG"
     gene            complement(1444..>1653)
                     /locus_tag="PLL14_10965"
     CDS             complement(1444..>1653)
                     /locus_tag="PLL14_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG04494.1"
                     /translation="HKQLIEKGMAASIAALSLDLRERDERDSQRSVAPMQQSADAELL
                     DTTSLTIETAVAQVLDWVNKVPKVG"
BASE COUNT          312 a          516 c          475 g          350 t
ORIGIN      
        1 cccttcttga agttgcgaat cgcctcttcg ccgggcaggg accacgacag gtcggacaga
       61 tgcaccagac cgtcgatgcc accgggcagg ccgatgaaca caccaaagtc ggtaatcgac
      121 ttgatcgccc cgcgcacccg gtcgcccttc ttgtagttca tcgagaagtc gtcccacggg
      181 ttggcagcgc actgcttcat gcccagggag atgcggcggc gatcttcgtc gatctcgagg
      241 atcatcacct cgacctcgtc gcccaactgc acgaccttcg aggggtgaac gttcttgttg
      301 gtccagtcca tctcggagac atgcaccagg ccctcgatgc cctgctcgat ctcgacaaag
      361 gcaccgtagt cggtgagatt ggtgaccttg ccgaaaaggc gtgtcgtcgg cggatagcgg
      421 cgcgagatgc cgacccaggg gtcttcgccc aactgcttga ggccgagcga aacgcggttc
      481 ttttcctggt cgaacttgag gatcttggcc tggacttcgt cgccgacggc cagcacttcc
      541 gaggggtgac gcacccggcg ccatgccagg tcggtaatgt gcagcaggcc atcgatacca
      601 cccaggtcga cgaaggcgcc gtagtcggtg atgttcttga ccaccccctt gacgatgctg
      661 ccttccttga gcgccgcgag cagcgcatcg cgctcttcgc cgacgctcgc ttcgagaacg
      721 gcgcggcgcg agaccaccac gttgttgcgc ttgcggtcga gcttgatgac cttgaattcg
      781 taagtcttgc cttcgtacgg cgtggtgtcc ttgaccgggc gcatgtcgac cagcgagccc
      841 ggcaggaagg cgcggacact gttggtcatc accgtcagcc cccccttgac cttgccggta
      901 atggtgccgg tgacgagcga accgtcgttc agcgcctgct cgagcgcatt ccacgcggcg
      961 acacgtttgg cacggtcgcg cgacagacga gtttcaccga aaccgttctc gagctgttcg
     1021 atggccacct ggacgaaatc gccgaccttg acttcgagtt cgcccttgtc gctcaggaat
     1081 tcatcaattt caatgtagct ctcggacttg agaccggcat tgaccaccac aaagttctgg
     1141 tcaatgcgca cgacttccgc agtgatgacc tctccgggac gcatttcctg acgcccgagg
     1201 ctctcttcga aaagttcggc aaagctttct tgcatggagg gattggctga catatgtgtt
     1261 gagttaccta accgcaatgc tgcggggtga gttgaaaaca atcaccagtt ccggtcactc
     1321 cggaaacctc acccaagcgg gaagggaaga cactgacccg gcagaacctg gtgcaatctg
     1381 cggtcaggcc tgccgaacga cgcgtcgagc tggtgacacc tctggcaaac ggcggttgcc
     1441 gttttaccct actttcggaa ccttgttgac ccagtccagc acctgtgcca ccgctgtttc
     1501 gatggtcagg ctcgtcgtgt cgagcaattc agcatccgca ctctgctgca tcggagcgac
     1561 gctacgttgg ctatcccgtt cgtcgcgctc gcgcagatcc agcgagaggg ctgcaatact
     1621 agcagccatt cctttttcga tcaactgctt atg
//
LOCUS       DAOSIT010000556         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64163_length_1648_cov_8.038293, whole genome shotgun sequence.
ACCESSION   DAOSIT010000556 DAOSIT010000000
VERSION     DAOSIT010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64163_length_1648_cov_8.038293"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLL14_10970"
     CDS             <1..473
                     /locus_tag="PLL14_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG04495.1"
                     /translation="LAPQPGERIADMFCGLGNFTLPIARAGARVVGVEGSPELVRRAT
                     GNALANGLGDRAEYRVANLFETTPETLAALGRFDRMLIDPPREGALELIKSLDAERPQ
                     RIVYISCSPATLARDAAILVTQKDYRLRGAGVVNMFPHTSHVESIALFERNTSP"
     gene            599..1330
                     /locus_tag="PLL14_10975"
     CDS             599..1330
                     /locus_tag="PLL14_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOG04496.1"
                     /translation="MTADSREERAPKQEAEGRGTTETAAERSVPLDEVKLAIGDSFQV
                     QPQTDLAQSRYYVKLIGYLKGKSVVTTIPEADGKLCYLRDGQTLVVRFFAGKNAYAFT
                     ANVLRSSSIPFPHMYLNYPPQVRGLVVRAGERVPVRIICAIDMPQETSTIAAAGLMTN
                     LSVGGALLATKTALGKKGALLSVKFRLHVRGIEVLMAIDATIRSQSQDASGEFLQGIE
                     FAGLPDATAIALTAFVYQTLAEASR"
     gene            complement(1347..1648)
                     /locus_tag="PLL14_10980"
                     /pseudo
     CDS             complement(1347..1648)
                     /locus_tag="PLL14_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019687989.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-ACP transacylase"
BASE COUNT          314 a          523 c          497 g          314 t
ORIGIN      
        1 tgctcgcgcc gcaaccgggc gagcgcatcg cggacatgtt ctgtggtctg gggaacttca
       61 ccctgccgat cgcgcgcgcg ggcgcccgtg tggtcggtgt cgaaggcagt cccgaactgg
      121 ttcgccgcgc caccgggaac gcgctggcaa atggtctggg cgatcgggcc gaatatcgtg
      181 tcgccaatct cttcgaaacg acgccggaaa cgctggcggc cctgggtcgc ttcgacagga
      241 tgctcatcga cccaccgcgc gaaggcgctc tggaactgat caagtccctg gatgccgagc
      301 gcccgcagcg catcgtctac atctcgtgca gcccggccac cctggcacgc gacgcggcga
      361 ttctggtcac gcagaaggac taccgcctgc gtggcgccgg cgtggtgaac atgtttcccc
      421 atacctcgca cgtcgagtca atcgccctat tcgaacgtaa cacctcgccc tgagcattca
      481 cccgccgtgg caattgcgcc acgcaggacg gccggcaacg atttctctcc cgagatatcc
      541 gctttgctga aagttcttta tgatctgtgc tttcagaccg ggccacggaa atccgacgat
      601 gaccgccgac agcagggaag aacgcgctcc gaagcaggag gccgaggggc ggggcacgac
      661 cgaaacagcg gccgagcgga gcgtacctct cgacgaggtc aagctggcca tcggcgattc
      721 cttccaggtt cagcctcaga ccgatctggc gcaaagccgg tactacgtca agctgatcgg
      781 ctatctcaaa ggcaagagcg ttgtgacaac gattccggaa gcagacggaa aactctgtta
      841 cctgcgcgac gggcagaccc tggtcgtacg ctttttcgcc ggcaagaatg cctatgcctt
      901 caccgccaat gtgctgcgtt cttcgagcat cccttttcca cacatgtatc tcaactatcc
      961 gccgcaggtg cgtggtctgg tggtacgcgc cggcgagaga gtcccggtca ggatcatctg
     1021 cgccatcgac atgccgcaag agacgagcac gattgccgcc gccggcctga tgaccaacct
     1081 cagtgtcggc ggcgccttgc tggcaacgaa gacggctctg gggaagaaag gcgcgctcct
     1141 gtccgtcaag tttcgcctgc atgtcagggg aatcgaggtg ctgatggcga ttgatgcgac
     1201 gattcgctca caatcgcagg atgcgtccgg agaattcctc cagggcatcg agttcgctgg
     1261 tctgcccgat gccacggcca ttgcgctgac cgcgttcgtc tatcagaccc tggcggaagc
     1321 gtcgcgctga cggcatcggc agtgccatag acctcggcta aggaacgctg gaaatacttt
     1381 tcctggatca tcctggcggt tcccggatcg atctgctcga tcgaatcgac cgacgaccgg
     1441 tacaactcgt agagcttgtt ggcgcgcacc gggaagaata ggccgcgttt agcacctgta
     1501 ccttggcgcc gatctcgaac atgtcgccga ccggcgcgta atcggtgtcc tgcacctgca
     1561 tttcctgcag ccggtccttg accagctcgc tgctacccgc ttcgaccgta cactggttga
     1621 tcgagccggt gaggatgaag tcggcacc
//
LOCUS       DAOSIT010000557         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64260_length_1646_cov_8.792583, whole genome shotgun sequence.
ACCESSION   DAOSIT010000557 DAOSIT010000000
VERSION     DAOSIT010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64260_length_1646_cov_8.792583"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLL14_10985"
     CDS             complement(<1..500)
                     /locus_tag="PLL14_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG04497.1"
                     /translation="MPTMPATSSPPAVSEILAAANAVILGKPAQTRLALCCLLAKGHL
                     LIEDLPGVGKTTLAHTLARLLGFQFARVQFTSDMLPADILGVSIFDRERGNFRFLPGP
                     VFSQVLLADEINRATPKTQSALLEAMEEGQVTTEGETRPLPQPFFVIATQNPSNQVGT
                     FPLPES"
     gene            610..1536
                     /locus_tag="PLL14_10990"
     CDS             610..1536
                     /locus_tag="PLL14_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOG04498.1"
                     /translation="MTSTAFISHSDCQLHDMGSYHPETPERLHAISDHMIAQGLDSYF
                     SYHEAPPATFQQLKRVHTAAHLERLKRAAPELGIVHLDPDTAMNRYTWQAALRAAGAG
                     VKAVDLVMAGDVENAFCAVRPPGHHAERAAAMGFCFFNNIAVAAAHALEAHHLSRVAV
                     IDFDVHHGNGTEDCFKKDPRVLMVSTFQHPFYPYSGTVSPAANMLNIPLGRGTDGEEF
                     RRVVSDLWMPRLHEFRPEMLFISAGFDGHYEDEMGGLKLLEKDYAWVTEQMKQVAEEH
                     AQGRIVSMLEGGYSLSALARSVVAHIRVLAAI"
BASE COUNT          311 a          456 c          544 g          335 t
ORIGIN      
        1 tgcgattccg gcaatgggaa agtccccacc tgatttgaag gattctgtgt ggcaatcacg
       61 aagaaaggct gcggcagcgg gcgcgtttcg ccctcggtgg tgacctgccc ctcttccatg
      121 gcttcaagca gggcgctctg cgttttcggc gtggcgcggt tgatctcgtc ggcgagcagc
      181 acctgggaaa acaccggtcc ggggaggaac ctgaagttgc cgcgctcgcg gtcgaagatc
      241 gagacgccga ggatgtccgc cggcagcatg tcgctggtga actggacgcg cgcgaactgg
      301 aatccgagca ggcgggcgag ggtgtgcgcc agggtggtct tgcccacgcc gggcagatct
      361 tcaatcagca agtggccttt tgccagcagg cagcagagtg caaggcgtgt ttgtgcgggt
      421 ttgccgagaa ttacggcatt ggcggcggca agaatctcgc tgactgctgg cgggctggaa
      481 gtggcaggca tggttggcat ggcttgtcgt ggtggcgcaa ttgggacgat aataaaccat
      541 tagcgaataa ctcgatttaa cgtggatcca ggcgccccgg cgactgccgt ctacgacaga
      601 aaggctacag tgaccagcac tgcattcatc agccacagcg actgccagtt gcacgacatg
      661 gggtcgtatc atccggaaac tccggagcgc ttgcacgcca tcagcgatca catgattgcg
      721 caagggctgg attcgtactt ttcctaccac gaagcgccgc cggcgacctt ccagcagctc
      781 aaacgcgtgc ataccgcagc ccatctggag cgtctgaagc gtgccgcgcc ggagttgggt
      841 atcgtccacc tggacccgga tacggcgatg aaccgatata cctggcaggc ggctttgcgc
      901 gctgccggtg cgggtgtcaa ggcggttgac ctggtgatgg ccggcgatgt cgagaatgcg
      961 ttctgcgcgg ttcgtccccc cggccatcat gccgagcgtg cggcggcgat gggcttctgt
     1021 tttttcaaca atatcgctgt cgctgcggcg catgcgctcg aagcgcatca cctgtcgcga
     1081 gtggcggtga tcgatttcga cgtgcaccac gggaatggta ccgaagactg cttcaagaag
     1141 gacccgcgcg tcttgatggt cagtaccttc cagcatccgt tctatcccta cagcggtacc
     1201 gtaagtccgg ctgccaacat gctcaacata ccgcttggga ggggtaccga cggcgaggag
     1261 ttccggcggg tggtgagcga cctctggatg ccgcgcctgc acgaattccg gccggaaatg
     1321 ttgttcattt cggcgggttt cgacggacat tacgaggacg agatgggcgg tctgaaattg
     1381 cttgagaagg actatgcctg ggtcaccgag cagatgaagc aggttgccga ggagcatgcg
     1441 caggggcgta tcgtgtcgat gctcgaaggc ggatactcgc tgtcggcgct ggcgcgaagc
     1501 gtagtggccc atatccgggt cctggcggcg atctgacccc tcgccacagc ggcaaggggt
     1561 gctcaccatc gtcggagaag cacgtttttt cgtcgccaat cggctggctt tcggttagaa
     1621 ttcagcgttt taatttccac ggcgaa
//
LOCUS       DAOSIT010000558         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64261_length_1646_cov_7.619736, whole genome shotgun sequence.
ACCESSION   DAOSIT010000558 DAOSIT010000000
VERSION     DAOSIT010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64261_length_1646_cov_7.619736"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1467)
                     /locus_tag="PLL14_10995"
     CDS             complement(25..1467)
                     /locus_tag="PLL14_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOG04499.1"
                     /translation="MPATTFKRSLLAASLIIAFGAGYAKFGGLAISPTEALAAPVAAT
                     PAALPRSGVTLPDFSAIVDGTGPAVVNISVSGRQKTAATMDPDDPMFEFFRRFGVPTP
                     QQRGPARGMGSGFIVSADGVILTNAHVVADADEVTVKLTDKREFKARVVGLDKLTDVA
                     VLRIEASNLPALRLGDPARTRVGEWVVAIGSPFGFENSVTAGIVSAKSRSLASDSYVP
                     FIQTDVAINPGNSGGPLLNLDGEVIGINSQIYSRSGGYQGVSFAIPIDVAMNIEQQLL
                     KDGKVSHGRMGVTIQEVSQSLAESFALKSTAGALVSSVEKSSPAAAAGLEPGDIILKL
                     NDTVIGRSSELPPLVAALKPGTQVRLHVWRKGTSRDLTLSVGEIAAPRLASARGGEAD
                     KSRLGLALRPLSPEERRQVENPEGLLVENVSGPAARAGIRPGDIIVSLNGQAIREISQ
                     VRSLLDKAGRVVALLIERDKVKIFVPVELG"
BASE COUNT          289 a          538 c          539 g          280 t
ORIGIN      
        1 tcgaacaggg cattgatcat cgcatcaacc gagctcgacc ggcacgaaga tcttcacctt
       61 gtcgcgctcg atcagcaagg ccacaacgcg gccggccttg tcgagcaggg agcgtacctg
      121 cgatatctcg cgtatcgcct gtccattgag cgacacgatg atgtcgccgg gccggatgcc
      181 ggcgcgcgcg gcgggtccgc tgacgttttc aacgagcaga ccctcgggat tctcgacctg
      241 ccgacgctcc tcagggctca gcgggcgcag cgccaggccg agtcgggact tgtcggcctc
      301 accgccgcgc gccgacgcca gccggggtgc cgcaatttcg ccgaccgaca gcgtcagatc
      361 acggcttgtt cccttgcgcc agacgtgcag ccggacctgg gtgccgggtt tgagcgcggc
      421 gaccagcggc ggcagttcgg aagaacgccc gatcacggta tcgttgagct tgagaatgat
      481 gtcgccgggt tcaagtcctg ccgccgctgc cgggctgctt ttttcgaccg aactcaccag
      541 ggcgccggcg gtactcttca gcgcaaagga ttcggccaga gactgcgaaa cctcctggat
      601 cgtcaccccc atgcggccat ggctgacctt gccatccttg agcagttgct gctcgatgtt
      661 catcgccaca tcgatcggaa tggcgaacga cacgccctga tagccgccgc tgcgactgta
      721 gatctgcgag ttgatgccga tcacctcgcc gtccaggttg agcagcggcc cccccgagtt
      781 gccggggttg atcgccacgt cggtctggat gaacggcaca tagctgtcgc tggccagcga
      841 acgcgatttt gccgagacga tgccggcggt gacgctgttc tcgaagccga acggcgagcc
      901 gatggcgacg acccattcgc caacccgtgt gcgtgccggg tcaccgaggc gcagggccgg
      961 cagattgctg gcctcgatgc gcagcaccgc gacatcggtg agcttgtcga ggccgacgac
     1021 ccgcgccttg aattcgcgct tgtcggtcag cttgacggtc acctcgtcgg catcggcaac
     1081 gacatgcgca ttggtcagga tcaccccgtc ggcgctgacg atgaagccgg aacccatgcc
     1141 gcgagccggc ccgcgttgct gtggagtcgg caccccgaag cgccgaaaga actcgaacat
     1201 cggatcatcg ggatccatcg tcgccgccgt cttctgccgc ccactgacgc tgatattgac
     1261 caccgccggg ccggtgccat cgacgatggc actgaagtcg ggcagcgtga cgcccgagcg
     1321 gggcagggcg gcaggcgtcg ccgcgaccgg cgcggcaagc gcctcggtcg ggctgatggc
     1381 gagtccgccg aacttcgcgt agccggcgcc gaaagcgatg atcagcgaag cggcgagcag
     1441 gctgcgtttg aatgttgtgg ctggcatggc aatctcctgt ttcgttgcac caagggaagc
     1501 actgggcttc cgacgggtaa acgatagacg gccgtgactt aaggctgact taaagcggag
     1561 tcagggcaac accgggcaac cctgctgatc gcttgaattg cacgcgcgcg cgcaggccgc
     1621 ccagcggcgc cgtgtccagc acgacg
//
LOCUS       DAOSIT010000559         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64272_length_1646_cov_3.408548, whole genome shotgun sequence.
ACCESSION   DAOSIT010000559 DAOSIT010000000
VERSION     DAOSIT010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64272_length_1646_cov_3.408548"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1264
                     /locus_tag="PLL14_11000"
     CDS             230..1264
                     /locus_tag="PLL14_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOG04500.1"
                     /translation="MNVPLWAWFGVLGVILVMLAIDLFAHRKAHVIGIKEAASWSALW
                     VALGVAFGGVIWWWAGAEYAGQYYAGYLIEKSLAIDNVFVWAIIFSYFAVPRQYQHRV
                     LFYGVIGALIFRGAFIAAGSALIASFAWILYVFGAFLLITGYTMIKKRADHIDPSKSK
                     VMGLFKRFVPSTDEYHGQKFWIKQGGKWVATPLLAVLVLVETTDIIFAVDSIPAIFAV
                     TQEPFLVFTANAFAILGLRAMYFLLADLMHRFVYLKLGLGLVLIWVGIKLVLQVDIYK
                     MPTWLSLGVVALIIATSIAASLFVTRRGDATPEMDSVADGDGRPIDDGHAEEVDLSDQ
                     SRSRTGLRAP"
     gene            1179..1475
                     /locus_tag="PLL14_11005"
     CDS             1179..1475
                     /locus_tag="PLL14_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HOG04501.1"
                     /translation="MGGRLMTDTRRKWTFLTNHGHVLVYVHRNSDARVRDIATAVGIT
                     ERAAQTILADLENDGYLRKTKVGRRNTYAVNTKGQFRHPEEAGHRIGDLLSLFQ"
BASE COUNT          303 a          504 c          499 g          340 t
ORIGIN      
        1 ggtttctcgg ggcgtgtcgc cgcaacaacc gccaatcgca gccggtcgca caatgcagcc
       61 cggcgggccg acttgacaca tgcacggcta ttcaggaaac ttgaaacaag cgaaggggag
      121 tatccacgcg cggtggtcgt cattacgact tcacatgaag tccggcgccg cggaccggga
      181 cctggtcgga aagaccttcg gatgttctta acgaccgaag gagtcatctg tgaacgtccc
      241 cctgtgggcc tggttcggtg tgctcggcgt gatcctggtc atgctggcca tcgatctgtt
      301 cgcgcatcgc aaggcgcatg tcatcggcat caaagaggcg gcgagttggt cggccctgtg
      361 ggtcgccctc ggagtcgcat tcggaggtgt catctggtgg tgggccggcg cggagtatgc
      421 cgggcagtac tacgccggct acctcatcga gaagtcactg gccatcgaca acgtcttcgt
      481 ctgggccatc atcttcagct acttcgccgt gccccggcag taccagcacc gggtgctctt
      541 ctacggcgtc atcggcgcgc tgatcttccg tggcgcgttc atcgccgccg gctcggcttt
      601 gatcgcctcg ttcgcctgga tcctgtatgt gttcggcgcc ttcctgttga tcaccggcta
      661 cacaatgatc aagaagcggg ccgaccacat tgacccgtcg aaatccaagg tgatgggtct
      721 gttcaaacgg ttcgtgccct cgaccgacga gtaccacggc cagaagttct ggatcaagca
      781 gggcggtaag tgggtcgcca cgccgctgct ggcggtcctg gtgctggtcg agacgaccga
      841 catcatcttc gccgtcgaca gcattccggc gatcttcgcg gtgacccagg aacccttcct
      901 ggtcttcacg gccaacgcct tcgccatcct cggcctgcgg gccatgtact tcctgctggc
      961 cgacctgatg catcgcttcg tctacctgaa actgggcttg ggtctggttc tcatctgggt
     1021 gggtatcaaa ctcgtactcc aggtggacat ctacaagatg cctacatggc tgagtctcgg
     1081 ggtggtcgcc ttgatcatcg ccacaagcat cgcggccagc ctcttcgtca cccgccgtgg
     1141 agatgcaaca ccagaaatgg atagtgttgc tgacggcgat gggaggccga ttgatgacgg
     1201 acacgcggag gaagtggacc tttctgacca atcacggtca cgtactggtc tacgtgcacc
     1261 gtaattccga tgctcgtgtg cgtgatatcg cgacagctgt agggatcacg gagcgtgccg
     1321 cgcagaccat cctcgccgat cttgagaacg acgggtacct gcgcaagacc aaggtaggac
     1381 gtcgcaacac ctacgcggtg aacaccaagg gtcagttccg gcatccggag gaagcaggtc
     1441 accgcatcgg tgacctgctt tcccttttcc agtaggaaat gccgatcgtc gagcgcaatc
     1501 cgttgccgcg gatcgcgctg cgcacgcgtg ccgaactacg ggcctggttc caggacaatc
     1561 acggcgatca tcgaggcttc tggctggtga gcagccgacc cggaccggac atcccctacg
     1621 acgacctcgt cgaggagtgc ctcatc
//
LOCUS       DAOSIT010000560         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64309_length_1645_cov_7.193711, whole genome shotgun sequence.
ACCESSION   DAOSIT010000560 DAOSIT010000000
VERSION     DAOSIT010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64309_length_1645_cov_7.193711"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..>1645
                     /locus_tag="PLL14_11010"
     CDS             277..>1645
                     /locus_tag="PLL14_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOG04502.1"
                     /translation="MALTAGEVPMLYMPADDSDPTNEADPGVLSWTEELRRVTVAVSK
                     KDRRPAASRQQLFYLLLWTADARGFGVTVHKGRDPESAEEMWNIDRALNKPPRFVSDE
                     DRAILRLLWAERSFDTGLRAFGLGPRHGGEALQLMAETGRLCRKDDFSTLTPAAPRSA
                     TLGWHENVDGRRLPMLVPDPPANLVVPLPVPWYVDLARRQIGPLQIPGNAAVVARLFS
                     LPPLSATAAALVGEALAEPACELPGDPEQASVAMRSIVAEPLPVLRLQTLGTHGNRSW
                     REYLASYGGGPFDVALPVFRYADVEVIPDDMRDFSTLASGEMVRIERQRAREDLLMDE
                     LAGSGLEKIPGYVLHTFGRPPENAYGLAFEGGWPAFMRNEVLRLRAVGWQVEFAADYR
                     HRLLEVEAWDADLVASENGWFDLDMGIIVEGERLPLAPLLAALFRRDGRWLDPGLLAQ
                     IADQEL"
BASE COUNT          249 a          542 c          556 g          298 t
ORIGIN      
        1 gcatcgctgc ggtgaccaag gcgatcaccg atttcccgat caagtacggc aattttcgcg
       61 actgagtagt gcatcagccg gccatacccg ccagcatgcc tttccctgct tgccgccgct
      121 tgtcggcatg tggcagctta cacggccggt atctgtccgg agcttcccgt tccgggcgcc
      181 gggaaggcgg gctaccaatg acggttctgt tgcgggttca cattcctgag gcgcttccga
      241 tcatcgtgcc gcgtcgattc ctgatgcggc gccgaagtgg cgctcactgc gggcgaggta
      301 ccgatgctgt atatgcccgc tgacgattcc gacccgacga acgaggccga ccccggcgtg
      361 ctgtcctgga ccgaggaact gcgccgggtg acggtggcgg tcagcaagaa ggaccgccgc
      421 ccggccgcca gccgccagca gcttttctac ctgctgctat ggaccgccga tgcgcgcggt
      481 ttcggcgtta ccgtgcataa ggggcgcgac ccggaaagtg ccgaggagat gtggaacatc
      541 gatcgcgccc tgaacaagcc gccgcgcttt gtcagcgacg aagatcgcgc catcctgcgc
      601 ctgttgtggg ccgagcgctc cttcgacacc ggtctgcgcg cctttggcct gggaccacgg
      661 cacggcggcg aggccctgca gctgatggcc gagaccggcc gcttgtgccg caaggatgat
      721 ttctcgacgc tgacgccggc agcgccgcgt tcggcgacgc tagggtggca cgagaacgtc
      781 gacgggcgcc ggctgccgat gctggtgccg gatccgccgg cgaacctggt cgtccccctg
      841 ccggtaccgt ggtatgtcga tcttgccaga cggcagatcg gccccctgca gattccaggc
      901 aacgccgccg tcgtcgcccg cctgttctcg ctgccgccgc tgtcggcgac ggcagcggcg
      961 ctggttggcg aagccctggc cgagccggcg tgcgagttgc ccggcgaccc cgagcaggcc
     1021 agcgtggcga tgcgcagcat cgtcgccgag ccgctgccgg tgctgcgcct gcaaaccctg
     1081 ggcacccacg gcaaccgcag ctggcgggag tatcttgcca gttatggcgg aggtcctttc
     1141 gatgtcgccc tgccggtgtt tcgctacgcc gatgtcgagg tgattcccga cgacatgcgc
     1201 gatttctcga cgctggccag tggcgagatg gttcgcatcg agcgtcagcg agcacgcgaa
     1261 gatctgctga tggacgaact ggccggctcc ggtcttgaaa aaattcccgg ttacgtcctg
     1321 cacaccttcg gcagaccacc tgaaaacgcc tacggcctgg cgttcgaagg cggctggccg
     1381 gcgttcatgc gcaacgaggt gctgcgcctg cgtgctgtcg gctggcaggt cgagtttgcc
     1441 gccgattacc ggcatcgcct gctcgaggtc gaggcctggg atgccgatct cgtggcgtcg
     1501 gaaaatggct ggttcgacct cgacatgggg atcatcgtcg aaggcgagcg cctgccgcta
     1561 gcacccctgc tggcggcgct gttccggcgt gacggacgct ggctcgatcc gggcctgctg
     1621 gcacagatcg ccgaccagga gctga
//
LOCUS       DAOSIT010000561         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64363_length_1644_cov_6.683449, whole genome shotgun sequence.
ACCESSION   DAOSIT010000561 DAOSIT010000000
VERSION     DAOSIT010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64363_length_1644_cov_6.683449"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLL14_11015"
     CDS             complement(<1..784)
                     /locus_tag="PLL14_11015"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG04503.1"
                     /translation="MNKPERKAPVHAPMHHFPVRDGVLVVGGIPLPQLAARVGQTPFY
                     AYDRQLLSQRVAELRTALPPGLKLHYAMKANPMPALVAHLSGLVDGIDVASSGELKVA
                     LDAGADAREISFAGPGKRREELQQAVAAGILINIESFREVSELAAIIDRTGYPARVAV
                     RVNPDFELKSSGMKMGGGARQFGVDAEQVPELLAEIGRSGIGFEGFHLFAGSQNLKPD
                     AIVEAQDRSFELALRLAQSAPSPVRFLNLGGGFGIPYFPGEQH"
     gene            complement(781..>1644)
                     /locus_tag="PLL14_11020"
     CDS             complement(781..>1644)
                     /locus_tag="PLL14_11020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG04504.1"
                     /translation="ADLLSLRDLLAAAARDHGGAHVMHFAPMDPESSTFHALLTGLRA
                     NDWIPFRFFCFGNWFLHVDGSWEDYLRQRSANLRSAIRRRCKRFAADGGTLHIATDPE
                     HLEHAIAEFQAIYLASWKKPEPYPDFVPALIRRLASMGMLRLGIARIGERTIAAQLWI
                     VDRQKASIYKVAYHEQFASYSPGTVLTAHLLQHVIDKDRVREVDFLIGDDPYKRMWMS
                     DRRERWGIVAYNPHTLIGCALLLKEIAGRTVRAAAAALRRVCLPARRLDSRNKATKTG
                     NCDKPHKGSRW"
BASE COUNT          306 a          509 c          505 g          324 t
ORIGIN      
        1 ggtgctgctc cccagggaag taggggatgc cgaacccccc cccgaggttg aggaaacgca
       61 ccggtgatgg cgccgactgt gcgagccgca gcgcgagttc aaagctcctg tcctgggctt
      121 ccacgatggc gtcgggcttg aggttctgcg acccggcaaa gaggtgaaag ccttcaaaac
      181 ctattcccga acggccaatc tcggccagca actcgggtac ttgctcggcg tctacgccga
      241 actgtctggc acccccgccc atcttcatcc ccgaactctt cagttcgaaa tccgggttca
      301 cgcggacggc aacacgcgcc gggtagccgg ttcggtcaat gatcgccgcc agctcggaga
      361 cttcgcgaaa ggattcaata ttgatcagga tgcctgcggc gaccgcctgt tgcagttcct
      421 cacgtcgttt gccgggcccg gcaaagctga tttcccgagc atctgcgccg gcatcgaggg
      481 cgaccttcag ttcgccgctc gacgccacgt caatcccatc gaccagcccg ctcaggtgag
      541 cgaccagggc cggcatcggg tttgccttca tcgcgtagtg cagcttcaag cccgggggca
      601 gggccgtccg caattcggcg acccgttgtg acaacagttg acggtcgtag gcgtaaaacg
      661 gtgtctgtcc gacgcgggcg gcgagttgcg ggaggggtat tccgccgacg acgagcacac
      721 cgtcccttac cgggaaatga tgcattggcg cgtgtacggg ggcttttcgt tccggtttat
      781 tcaccagcgg cttcctttgt gtggcttgtc gcagttgcct gttttcgtgg ccttgtttcg
      841 ggagtcaagt ctcctggctg gcaagcagac ccggcgcaag gctgccgcgg ccgctctgac
      901 cgtacgcccc gcgatctcct tgagcaacaa ggcacagccg atcagggtat ggggattata
      961 ggcaacgatt ccccagcgtt cgcggcggtc actcatccac atccgtttgt atgggtcgtc
     1021 gccgatcagg aagtctacct cccggacgcg gtccttatcg atgacatgct gcagcaggtg
     1081 cgcggtgagt accgttcccg gagagtagga agcaaactgc tcgtgatacg ccaccttgta
     1141 gatgctggcc ttctgacggt cgacgatcca cagttgcgcc gcaatggtac gctccccgat
     1201 ccgtgcaatt ccgaggcgga gcatccccat gctggccagc cggcggatca acgcgggtac
     1261 gaaatcagga tagggttcgg gctttttcca gcttgccagg tagatcgcct ggaattcggc
     1321 gatcgcgtgc tcaaggtgtt ccgggtccgt ggcgatgtgc aaggttccac cgtcggcagc
     1381 aaaccgcttg caccttcgcc tgatcgcgct gcgcaggttg gcactgcgct gtctgagata
     1441 gtcctcccaa ctcccatcga catggagaaa ccagttgccg aaacagaaaa accggaacgg
     1501 gatccagtca ttcgcacgca agccggtgag cagcgcatga aatgtcgatg actcgggatc
     1561 catcggggca aagtgcatca cgtgcgcgcc gccatggtct cgcgcagcgg cggcaagcag
     1621 atcccgcaga ctcagcaggt cggc
//
LOCUS       DAOSIT010000562         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64505_length_1641_cov_11.227617, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000562 DAOSIT010000000
VERSION     DAOSIT010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64505_length_1641_cov_11.227617"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLL14_11025"
     CDS             <1..1107
                     /locus_tag="PLL14_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04505.1"
                     /translation="ADILLEDDKRTAAQIANAEKLRDALRSAFQESVAASKKAADEAK
                     KLLEQAATTRATGADKAAEIRRAQLPQDQQDTANANDFRDTADAATLAALEAKFAAQN
                     GRVDAAAKLAEQAIKGAERARGIIGKLADPGQQARATERLAEAEATAQEAQAKIKERQ
                     AKDAGDVATQQADRIKTLDAQIADLQKKAGEISVQVKIDEALGTIANLKAELASIDDK
                     TVNVVVKTTQTGGGTPAEDYAAYAAAADFRKEGFARGGYTGPGGKWQPAGVVHAGEFV
                     LRQEVVRQRGMLAMLQRLNREGLAALPGFASGGLVGALQISQARQPAPGAARAAAVFN
                     FPDLGRFPVTMDADPLRQLESAFARVALQKGGRR"
     gene            1104..1625
                     /locus_tag="PLL14_11030"
     CDS             1104..1625
                     /locus_tag="PLL14_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04506.1"
                     /translation="MSTLLKIGSLAIPNRAALDLDQTYETLGGETILRTVSGAAIKQE
                     TWRRLRTTLSGGGWLPAGLETINTSITQTIACIVPRALIADASRQATLPVGRRADAGH
                     QPFALALLDFGDVVPTPLAIAGNLATADAVAGAIGYQILYFPLLTCWVQRPSESGSRG
                     EASYRWELVAEEV"
BASE COUNT          309 a          586 c          540 g          206 t
ORIGIN      
        1 gctgacatcc tcctcgagga cgacaagcgc acggccgcgc agatcgccaa cgccgagaaa
       61 ctgcgcgacg cgctgcgcag cgcttttcag gaaagcgtcg ccgccagcaa gaaagccgcc
      121 gacgaagcca agaagctgct cgaacaagcg gcgacgacgc gcgccacggg cgccgacaag
      181 gccgccgaga ttcggcgcgc gcaattgccg caggaccagc aggacaccgc caacgcgaac
      241 gactttcgcg atacggcaga cgcggcaacg ctggccgcgc tcgaagccaa gtttgccgcc
      301 cagaacggcc gcgtcgatgc cgccgccaag ctcgccgagc aggccatcaa gggcgccgag
      361 cgcgcgcggg gaatcatcgg caagctcgcc gacccagggc agcaggcgcg cgctaccgag
      421 cgccttgccg aagccgaggc gacggcgcag gaggcgcaag ccaagatcaa ggagcggcag
      481 gccaaggacg ccggcgacgt ggccacgcag caggcggacc gcatcaagac gctcgacgcg
      541 cagatcgccg acttgcagaa gaaggccggc gagatcagcg tgcaggtgaa gatcgatgag
      601 gcgctcggaa ccattgccaa cctcaaggcg gagttggcat ctatcgatga caagacggtc
      661 aacgtcgttg tcaagacaac ccaaaccggc gggggcacgc ctgccgagga ttacgcggcc
      721 tacgccgccg cggccgattt ccgcaaggaa ggcttcgccc gaggtggcta caccggcccc
      781 ggaggaaaat ggcagccggc cggcgtggtg catgcgggtg agttcgtgct tcggcaggag
      841 gtggtgcgcc agcgcggcat gctggccatg ctgcagcggc tcaatcggga aggcctcgcc
      901 gcgcttcccg gcttcgccag cggcggcctg gtcggcgcgc tgcaaatcag ccaggcacgg
      961 cagccggcgc cgggcgctgc gcgcgctgct gcggtcttca acttccccga cctggggcgc
     1021 ttcccggtga ccatggacgc cgacccgctg cgccagcttg agtcagcgtt cgcccgggtc
     1081 gcactgcaga aaggcggccg gcgatgagca cgctactgaa aatcggctcg ctggctatcc
     1141 cgaaccgcgc cgcgcttgat ctggatcaaa cctacgaaac cctgggcggc gagacgatcc
     1201 tccgcaccgt ctcgggggcc gccatcaagc aagagacctg gcgccgcctg cgcaccacac
     1261 taagtggtgg cggctggttg ccggccggcc tggaaaccat caacacttcg atcacgcaaa
     1321 ccatcgcctg catcgtcccg cgcgcgctga tcgccgacgc cagccgccag gccacgctac
     1381 ccgtcggccg ccgcgccgac gccgggcacc agccgttcgc gctcgccctg ctggacttcg
     1441 gcgacgtcgt tccgaccccg ctggccatcg ccggcaacct cgccaccgcc gatgccgtcg
     1501 ccggcgccat cggctatcag attttgtact tccccctgct cacctgctgg gtgcagcgcc
     1561 ccagcgaatc cggatcccgc ggcgaagcca gctatcgctg ggaactggtc gccgaggagg
     1621 tttgacatgc cagacaccta c
//
LOCUS       DAOSIT010000563         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64569_length_1640_cov_8.153312, whole genome shotgun sequence.
ACCESSION   DAOSIT010000563 DAOSIT010000000
VERSION     DAOSIT010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64569_length_1640_cov_8.153312"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1393)
                     /locus_tag="PLL14_11035"
     CDS             complement(161..1393)
                     /locus_tag="PLL14_11035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOG04507.1"
                     /translation="MSSLESTLALPSRPGAKSQPRSAGAVLALLERIELGTLEIRLPD
                     GSLLRFGQTGPVVATLEVADWSMFERILERGDVGFADAWIDGEWHSPDLAGLLTLLAN
                     NRQCLARAVYGQWWSLLTARLRHWLNANTRAGSRRNIMAHYDLGNDFYRQWLDPTMSY
                     SSALYASDAPCSMAAAQLAKYRRILQRLNSRPGQSVLEIGCGWGAFAETAAREAELDV
                     VGLTLSPAQLQFARRRIQLAGLEQQVTLELRDYRDLAGEQFDHIVSIEMLEAVGERWW
                     PTYFTTLRRLLAPDGRAVVQVITIRDDLFARYRQGTDFVQQYIFPGGMLPSPSVVFQQ
                     AVRAGLVVRDVFAFGRDYARTLAEWSGNFERQWPAIEAQGFDERFRRLWHFYLGYCQA
                     SFNSGCTDVVQFELAHVR"
     gene            complement(1390..>1640)
                     /locus_tag="PLL14_11040"
     CDS             complement(1390..>1640)
                     /locus_tag="PLL14_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HOG04508.1"
                     /translation="DGNATWRLARIDHGDAAGDLLHTAISGNAEPLTSGPLLKAFLRF
                     PLLTLGVILRIHWQALKLWRKHVPFYRKPAPPLEETTR"
BASE COUNT          319 a          539 c          516 g          266 t
ORIGIN      
        1 ccggcgaccc acagcgtcgc gcgatagatc ggaaagccga gccagctcat ttcgctacgt
       61 ccccactgcc accagttggc ttccgacagg cgcaccgcat ccggcagggc ctgcgccaga
      121 ccactgcaag tccagagcag cagcgccatc agaggggttt tcatcggacg tgtgccagtt
      181 cgaactgcac cacgtcggta caaccgctgt tgaagctggc ctggcaatac cccagataga
      241 agtgccatag acgacgaaaa cgctcgtcga agccctgcgc ctcgattgcc ggccattgcc
      301 gctcgaagtt gccggaccat tcggccaacg tgcgcgcgta gtcgcgaccg aaggcaaaca
      361 cgtcgcgcac caccagccca gcccgaaccg cctgctggaa gacgaccgac ggactcggca
      421 acatgccgcc aggaaagatg tattgctgga cgaaatccgt cccctgacgg tagcgcgcga
      481 acaggtcgtc acgaatcgtg atgacctgca cgaccgcccg cccgtcgggt gccaacagcc
      541 gtcgcagggt tgtgaagtaa gtcggccacc agcgttcgcc gacggcttcc agcatctcga
      601 tcgaaacgat atggtcgaac tgctccccgg ccaggtcgcg atagtcgcgc aactccagag
      661 tcacctgttg ttcgaggccg gcgagttgga tgcgccgacg agcaaactgc agttgcgccg
      721 gcgacagggt cagaccgacg acatcgagtt cggcctcgcg tgcagcagtt tccgcgaaag
      781 cgccccagcc acagccgatt tcgagcacgc tctggcctgg cctggaattc aggcgttgca
      841 ggatgcgccg gtacttggcg agttgcgcag cggccatcga acacggcgca tcgctcgcgt
      901 acagcgcgct cgaatagctc atcgtcggat ccagccactg tcggtaaaag tcgttgccga
      961 gatcgtaatg ggccatgatg ttgcggcgcg agccagcgcg tgtgttggca ttgagccagt
     1021 ggcgcaggcg cgccgtcagc agactccacc attgaccgta gaccgctcgc gcgaggcact
     1081 ggcggttgtt ggccagcagg gtcagcaggc cggccaggtc cgggctgtgc cattcgccgt
     1141 cgatccaggc atcggcgaag ccgacatcgc cacgttccag aatgcgttcg aacatcgacc
     1201 agtcagcgac ctcgagtgtc gccacgaccg gccccgtctg gccgaagcgc agcaacgatc
     1261 cgtcgggcag gcgaatttcc agcgttccga gttcgattcg ttccagcagc gccagtaccg
     1321 ccccggcact acgcggctgc gacttggcgc caggcctcga aggcagcgcc agcgtggatt
     1381 ccaggctgct catcgcgtcg tctcctcaag tggaggcgca ggcttgcggt agaaaggcac
     1441 gtgcttgcgc cacagcttca gtgcctgcca gtggatgcgc agaatgacgc cgagcgtcaa
     1501 cagcgggaaa cggagaaagg ccttgagcaa gggcccactg gtcagcggct cggcattccc
     1561 ggagatcgcg gtgtgcagca ggtcacccgc ggcgtcgccg tgatcgatgc gcgccagccg
     1621 ccaggtggcg ttgccgtctt
//
LOCUS       DAOSIT010000564         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64570_length_1640_cov_8.133754, whole genome shotgun sequence.
ACCESSION   DAOSIT010000564 DAOSIT010000000
VERSION     DAOSIT010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64570_length_1640_cov_8.133754"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLL14_11045"
     CDS             complement(<1..188)
                     /locus_tag="PLL14_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG04509.1"
                     /translation="MPYPEKTAKPAAKDGTPPAAAGPAPARATVAIAFVQGMLAGLEH
                     TGRDTTALLERAHIPTQVL"
     gene            357..>1640
                     /locus_tag="PLL14_11050"
     CDS             357..>1640
                     /locus_tag="PLL14_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG04510.1"
                     /translation="MTDLSIAKKLLPYKPKHKVRFVTAASLFDGHDASINIMRRILQS
                     TGAEVIHLGHNRSVQEIVNAALQEDVQGIAITSYQGGHVEFFKYMIDLLRAGGGENIQ
                     VFGGGGGVIVPSEIDELHAYGVTRVYSPQDGQSMGLQGMINEVVTKSDFDLSALAPQD
                     PEDVLSALKAGDRRKLAQAITALENGAYPEALRKKILHAAAGLKVPTLGITGTGGAGK
                     SSLTDELVRRFRLDQGDTVKLAIISIDPSRKRTGGALLGDRIRMNAIEHPNIYMRSLA
                     TRETGSELSAALPEVIAACKLAGFDLVIVETSGIGQGNAAIVPLVDVSLYVMTPEFGA
                     ASQLEKIDMLDFADFVAINKFDRKGAEDALRDVRKQYQRNHEAFSQSPDEMPVFGTMA
                     ARFNDDGVTGLYQAIVPRLQALGLKFKRGKLPLVNV"
BASE COUNT          307 a          491 c          511 g          331 t
ORIGIN      
        1 agcacctgcg tcggaatgtg cgcgcgttca aggagcgcgg tggtgtcacg gccggtgtgt
       61 tcaagcccag ccagcatccc ctgcacgaag gcaatggcca ccgtggcgcg tgccggggcc
      121 gggcccgcgg cggctggcgg cgtaccgtct ttggccgcag gcttggcagt cttctcaggg
      181 tatggcattg gcttctcgca agatggtgta ttgacagcat atttcaagtt ggcaattttt
      241 acattgatag cggcgtattg ggtaatggct ttcttgctgt caacttccta ccatgtatgt
      301 ccccgtgcat ttcccggcaa ccacgacctt tctgattttc atggagcgcg aacgacatga
      361 ccgacctttc catcgccaag aagcttctgc cctacaagcc caagcacaag gtgcgttttg
      421 tcaccgccgc ttcgttgttt gacgggcacg acgcgtcgat caacatcatg cgccgcattc
      481 tgcagtcgac cggtgccgag gtcatccacc tcggccacaa ccgctcggtg caggagattg
      541 tgaacgcggc gctgcaggag gatgtgcagg gaatcgccat cacttcctac cagggggggc
      601 atgtcgagtt cttcaagtac atgatcgacc tgctgcgtgc cggtggtggc gagaacatcc
      661 aggtcttcgg cggtggcggc ggggtgatcg tcccgagcga gatcgacgag ttgcacgcct
      721 acggtgtgac ccgcgtctat tcgccgcagg acggtcagtc gatgggcttg cagggaatga
      781 tcaacgaggt ggtgacgaag tccgacttcg acctctcggc gctggcgccg caggatcctg
      841 aggacgtgct cagcgcgttg aaggcgggtg atcgccgcaa gctggcgcag gccatcaccg
      901 cgctcgaaaa cggcgcctac cccgaggccc tgcgcaagaa aatcctgcat gccgctgccg
      961 gcctcaaggt acctacgctc gggattaccg gtacgggtgg tgccggcaag tcgtcgctga
     1021 ccgacgaact ggtccgccgt ttccggctcg atcagggtga taccgtcaag ctggcgatca
     1081 tctccatcga tccttcgcgc aagcgcaccg gcggagcctt gctcggcgac cgcatccgca
     1141 tgaacgcgat cgagcacccg aatatctaca tgcgttcgct ggcgacacgc gaaacgggtt
     1201 ccgaactctc cgcggcactg ccggaagtga ttgccgcctg caaactggcg ggttttgatc
     1261 tggtcatcgt cgaaacttcg ggaatcgggc agggcaatgc agcgatcgtg ccgctggtcg
     1321 acgtttcgct ctacgtgatg acgccggagt tcggcgccgc ctcgcaactc gaaaagatcg
     1381 acatgctcga cttcgccgac tttgtggcga tcaacaaatt cgaccgcaag ggcgccgagg
     1441 atgctctgcg cgatgtgcgc aagcaatatc agcgcaacca cgaggctttc agccagagcc
     1501 cagacgaaat gcccgttttc ggcaccatgg ccgcgcgttt caacgacgat ggcgtgaccg
     1561 gcctgtatca ggccattgtg cccaggttgc aggcgctggg gctgaagttc aagcgcggca
     1621 aactgccgct ggtgaatgtt
//
LOCUS       DAOSIT010000565         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64630_length_1639_cov_6.490530, whole genome shotgun sequence.
ACCESSION   DAOSIT010000565 DAOSIT010000000
VERSION     DAOSIT010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64630_length_1639_cov_6.490530"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="metG"
                     /locus_tag="PLL14_11055"
     CDS             <1..890
                     /gene="metG"
                     /locus_tag="PLL14_11055"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOG04511.1"
                     /translation="HFAGQLCPCDESLPAIQAIRARRAAIAEYYEARDFGKAIREIMA
                     LIDIANQYVDSVKPWELARQAGREHELQAACSNALNLFRLFAILLKPVLPALASRAEA
                     FLNVAPFVWDDSATVLAAGHAINAYRHLLTRVDKKQVEALVAANRDSLAATPQAAPQQ
                     HAQQQEHAATVAVAQAAVGAAAVPHIGIDTFMNVDLRIARIQEATQVEGADKLVRLLL
                     DIGPLGSRQVFAGIKSAYDPATLIGRLTVMVANLAPRKMKFGMSEGMVLAASDAEGQT
                     PGIFLLSPDAGAQPGMRVK"
     gene            899..1153
                     /locus_tag="PLL14_11060"
     CDS             899..1153
                     /locus_tag="PLL14_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04512.1"
                     /translation="MNVGQRPQLTAKVMGYALLDAVGMLVFASGLMWLARHETLFIPG
                     FPTGTSTAVLTVVAGIALMVWSAARILRELARTSSDREQV"
     gene            1384..>1639
                     /locus_tag="PLL14_11065"
     CDS             1384..>1639
                     /locus_tag="PLL14_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOG04513.1"
                     /translation="MRKLEEQGYEVFLPLLTQWEKKKAGWTRKQQVMFPRYGFVRCSR
                     SGQSIAPIRSTPGVTGLVTFGNVPATLQEPTLDAIRSLAER"
BASE COUNT          286 a          559 c          506 g          288 t
ORIGIN      
        1 aacactttgc cggccagtta tgcccctgtg acgaatcgct gccggcgatc caggccatcc
       61 gcgcgcggcg tgcggcgatt gccgaatact acgaagcgcg cgacttcggc aaggcgattc
      121 gcgagatcat ggccctcatc gacatcgcca atcagtacgt cgacagcgtc aagccctggg
      181 aacttgcccg tcaggcgggt cgcgagcacg agctgcaggc cgcatgcagc aacgcgctga
      241 acctgttccg gctgttcgcc attctgctca agccggttct gccagcactc gcgtccagag
      301 ccgaagcctt cctcaacgtc gcccctttcg tctgggacga cagcgcgacg gtgctcgccg
      361 ccgggcatgc gatcaacgct tatcggcacc tgctgacgcg agtcgacaag aaacaggtcg
      421 aggctctggt cgcggccaac cgcgattcgc tggcagcgac tccgcaagcg gcgccacaac
      481 aacacgccca gcagcaggag cacgcggcaa cggtcgccgt cgcgcaggcc gctgtcggag
      541 cggccgccgt gccgcacatt ggcatcgaca ctttcatgaa cgtcgacctg cgcatcgcac
      601 gcatccagga ggcgacgcag gtcgaaggcg ccgacaaact cgtgcgtctg ctgctcgaca
      661 tcggcccgct cggctcccgg caggttttcg ccggcatcaa gtcagcctac gatccggcga
      721 cgctgatcgg tcgactgacg gtgatggtcg ccaacctggc gccgcgcaag atgaaattcg
      781 gaatgtccga gggcatggtc ctggccgcct ccgacgccga agggcagacg cccggcatct
      841 tcctgctctc gcccgatgcc ggcgcgcagc ccggcatgcg cgtcaaatag accggcacat
      901 gaacgtcggc caacgtccgc agttgacggc gaaagtgatg ggctatgcgc tgcttgacgc
      961 cgtcggcatg ctcgtcttcg ccagcggcct gatgtggctg gcccggcacg agaccctgtt
     1021 cattcccggt ttcccgactg gcacgagcac ggcggttctc accgtcgttg ccggtatcgc
     1081 cctgatggtc tggtcagcgg cccggattct gcgcgaactg gcgagaacct cgagcgaccg
     1141 cgagcaggtc tgaatccgtg gccgccgccc ggcgttcgct gatcacacgg cgctcaggcc
     1201 gtggttggcg attgactgct gcggccgatt ccggagatac ttacgctccc cctgaaattc
     1261 cgattcccct tcgctgcttc tgcgagtcgc acgcagcatt cccgtcgttg ccccgtgtct
     1321 tcgtccctgc catcaaaacc gtggtatgtc tgtctcacca agcctcggcg cgaagcactc
     1381 gcggtgcgca agctggaaga gcagggttac gaggtgtttc tgcccctgct gacgcagtgg
     1441 gaaaagaaga aagcgggctg gaccaggaaa cagcaggtga tgttcccccg ctacggtttt
     1501 gtacgttgca gtcgatccgg gcagagcatc gctcccatcc gcagcacgcc cggcgtcacg
     1561 ggtctggtca ccttcgggaa cgtgccggcc accctgcagg aaccgacgct ggacgccatc
     1621 cgttcgctgg cagagcgcc
//
LOCUS       DAOSIT010000566         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_64894_length_1634_cov_10.799873, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000566 DAOSIT010000000
VERSION     DAOSIT010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64894_length_1634_cov_10.799873"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1634)
                     /locus_tag="PLL14_11070"
     CDS             complement(<1..>1634)
                     /locus_tag="PLL14_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOG04514.1"
                     /translation="VAAMGDKIASKKLAMEARVNTIPGYNAEITDSAQAVEIAKGIGY
                     PVMIKASAGGGGKGLRVAFNDKEAFEGFEACRNEARNSFGDDRVFIEKFVEGPHHIEI
                     QVIADSFGNTVYLLERECSIQRRHQKVLEEAPSPFIDDSIRKAMGEQAVALAKAVNYQ
                     SAGTVEFVVGSDKSFYFLEMNTRLQVEHPVTEMITGVDLVELMIRVAAGEKLPFTQDD
                     IKPRGWAIECRINAEDPFRGFLPSIGRLVKYRPPATTDGEVRVDTGVFEGGEISMYYD
                     SMIAKLICHGSTRDQAINRMRDALNAFVIRGIDSNIPFQAALMQNARFLSGTFTTSFI
                     ADEYPSGFVAADVVHSDPGLLAAVAAFGRRRYIDRAVKVSGQMPGHERKVGSEWVVQI
                     EGKDYPVVLHPIPGGYSVTHGTDRYDLVSDWRFGELVFHGTCNGAPLCMQLERIGLLY
                     RVVHFGKQVKATVMTANAARMLALMPKKVPPDLSKFLLSPMPGLLREISVAEGEAVAA
                     GEKLAVIEAMKMENVLKAERDCKVRKVVAKAGESLSV"
BASE COUNT          276 a          512 c          527 g          319 t
ORIGIN      
        1 accgacaggc tttcgccggc cttggcgacg actttcctga ccttgcagtc gcgctcggcc
       61 ttgaggacgt tttccatctt catcgcttcg atcaccgcca gtttctcgcc ggcggcgacg
      121 gcttcgcctt ccgccaccga gatttcccgc agcaggcccg gcatcggcga cagcaggaac
      181 ttggagagat ccggcggcac cttcttcggc atcagcgcca gcatgcgcgc ggcgttggcg
      241 gtcatcaccg tggccttgac ctgcttgccg aagtggacca cccgatagag caggccgatg
      301 cgttcgagtt gcatgcacag cggcgcaccg ttgcaggtgc cgtggaagac gagttcaccg
      361 aaacgccagt cggagaccag gtcgtaacgg tcggtgccgt gggtaaccga gtaaccgcca
      421 ggaatcgggt gcaggacgac cgggtagtcc ttgccttcga tctggaccac ccactcgctg
      481 ccgaccttgc gctcgtggcc cggcatctgg ccactgacct tgaccgcgcg gtcgatgtag
      541 cggcggcgac caaaagcggc gacggcggcc agcaggccgg gatcgctgtg tacgacatcg
      601 gcggcgacga agccgctggg atattcgtcg gcgatgaacg aggtggtgaa ggtgcccgac
      661 aggaagcgcg cgttctgcat cagcgccgcc tggaagggta tgttcgagtc gatgccgcga
      721 atcacgaaag cgttgagcgc gtcgcgcatg cggttgatcg cctggtcgcg ggtgctgccg
      781 tggcagatca gcttggcgat catcgagtcg tagtacatcg agatttcgcc gccttcgaag
      841 acgccggtat cgacgcggac ttcaccgtcg gtggtcgccg gcgggcggta cttgaccagc
      901 cggccgatcg acggcaggaa gccgcggaac gggtcctcgg cgttgatccg gcactcgatc
      961 gcccaacccc tgggcttgat gtcgtcctgg gtgaacggca gcttctcgcc ggcggccaca
     1021 cgaatcatca attcgacgag atcgacgccg gtgatcattt cggtcaccgg atgctcgacc
     1081 tgcagacggg tgttcatttc gaggaagtag aacgacttgt cggagccgac gacaaactcg
     1141 acggtgcccg cgctctggta gttcaccgcc ttggcgagtg ccaccgcctg ctcacccatg
     1201 gccttgcgga tgctgtcgtc gataaatggc gagggcgctt cttcgaggac tttctggtgc
     1261 cgccgctgga tcgaacactc gcgttcgagc aggtagacgg tattgccgaa agagtcggcg
     1321 atgacctgga tctcgatgtg gtgcggtcct tcgacgaact tctcgatgaa cacgcgatcg
     1381 tcgccgaagc tgttgcgcgc ttcgttgcgg caggcttcga agccctcgaa ggcttccttg
     1441 tcgttgaagg cgacgcgcag tcccttgccg ccgccacccg ccgaggcctt gatcatcacc
     1501 gggtaaccga tccccttggc gatttccacg gcctgcgcgg aatcggtgat ctcggcgttg
     1561 tagcccggaa tggtgttcac ccgggcttcc atggccagct tcttggaggc gatcttgtca
     1621 cccatcgccg ccac
//
LOCUS       DAOSIT010000567         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65066_length_1631_cov_6.237310, whole genome shotgun sequence.
ACCESSION   DAOSIT010000567 DAOSIT010000000
VERSION     DAOSIT010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65066_length_1631_cov_6.237310"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLL14_11075"
     CDS             complement(<1..571)
                     /locus_tag="PLL14_11075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG04515.1"
                     /translation="MKPALSLALAAGMATLATIGWLGYRLLDQNRSPAGIPPGLVASQ
                     ELPPEQAQAAADAIMALTLPDLDGRPQAMAQWRGKVLVVNYWASWCAPCVEEMPAFSR
                     LQSRYAAQGVQFVGIGIDELKNMRTFVKANAVAYPLLVGDAAGTQTPALQVRGLPYTV
                     VIGRDGQLAHSRLGRLDEARLEPILQRLSL"
     gene            complement(568..>1631)
                     /locus_tag="PLL14_11080"
     CDS             complement(568..>1631)
                     /locus_tag="PLL14_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HOG04516.1"
                     /translation="GWGGFGLAALGTALMSVSPFFPDAEPVLNNYIPVLKQPLFFVSI
                     WTFGAGLSLTVLRALLTTWPQPFLAEPLRLGAFLGAVAAFLSLGAFVWSWAMVPWIEG
                     VIYYEVVFWGGGHTLQFQHALLMVVAWLWIATRLGSPSVASARALSFLFLVAALPLLL
                     VPAIYLLVPAGSLPHMELFAKLMLWGHPYMGPLILLGAVSLWALRGRPADPAKSALLA
                     SFTLFALGGVLGYLIQGVNVVIPAHYHGSTVGVTLAFMGLAYVLLPTLGFGRAEGRMA
                     IWQPYVYGAGQLIHVLGLAWSGGYGVQRKVAGAEQMLTTLPQKVGMGMMGLGGLIAVI
                     GGLMFVLVCLQAMSRRGER"
BASE COUNT          372 a          576 c          477 g          206 t
ORIGIN      
        1 gcaagctcag ccgttgcagg atcggctcaa gcctggcttc gtccaggcga cccaggcgac
       61 tatgtgcgag ttgcccatcg cggccgatca ccaccgtgta gggcagtccc ctgacctgca
      121 gtgccggggt ctgagtgccc gcagcgtccc caaccagcaa gggatacgcc actgcattgg
      181 ccttgacgaa agtccgcatg tttttcagtt catcgatacc gatgccgaca aactgcacac
      241 cctgcgcagc gtagcggctc tgcaggcgcg aaaaggcggg catttcctcg acacaggggg
      301 cgcaccagct ggcccagtaa ttgacgacca gaaccttgcc tcgccactgt gccatcgcct
      361 gcggacgtcc atcgagatcg ggtagcgtca gcgccatgat cgcgtcggcc gcggcctgcg
      421 cctgctccgg cggtagctcc tgcgacgcga ccaggccggg aggaataccg gccggcgaac
      481 ggttctgatc gagcaaacga tagcccagcc agccgatggt cgccagcgtc gccataccag
      541 ccgccagcgc cagactcaac gcaggcttca tcgctcgccg cgccgcgaca tcgcctgcag
      601 gcagaccaga acgaacatca gtccgccgat gaccgcgatc agccccccga ggcccatcat
      661 gcccatgccg accttctgcg gcagcgtcgt cagcatctgc tcggcgccgg caaccttgcg
      721 ctgcacaccg tagcctcccg accacgccag ccccagaaca tggatcaact gacctgcgcc
      781 atagacgtac ggttgccaga tcgccatccg gccctcggca cggccgaatc ccagcgtcgg
      841 cagcagtaca taggcgagac ccatgaaagc cagcgtgacg ccgaccgtcg aaccatggta
      901 gtgggcaggg atgaccacgt tgaccccctg gatcaaataa cccagcacac cacccagcgc
      961 aaacagcgtg aaggaggcca gcagggcgga tttggcgggg tccgccggcc ggccgcgcaa
     1021 agcccacagt gaaactgcgc cgagcagaat cagcggcccc atgtagggat ggccccagag
     1081 catcaacttg gcgaaaagct ccatgtgcgg cagcgaaccg gctggcacca gcagatagat
     1141 ggccggcacc agcagcagcg gcagggcagc caccagaaac agaaacgaca acgcgcgcgc
     1201 cgacgcgacc gacgggctgc caagccgtgt cgcgatccac aaccaggcga cgaccatcag
     1261 cagtgcatgc tgaaactgca gcgtgtgccc gccgccccag aatacgacct cgtaatagat
     1321 caccccctcg atccagggaa ccatcgccca cgaccagacg aaagcgccca acgacaggaa
     1381 ggccgcgaca gcacccagaa aagcacccag ccgcagcggc tcggccagaa aaggttgtgg
     1441 ccaggtggtc agcagcgccc gcagcaccgt aagactcaag ccggcaccaa aggtccagat
     1501 cgagacaaag aacaggggct gtttgagcac cggaatgtag ttgttgagca ccggctcggc
     1561 gtccggaaaa aacggcgaga cactcatcag cgccgtcccc agcgccgcca gcccaaagcc
     1621 accccagccc a
//
LOCUS       DAOSIT010000568         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65126_length_1630_cov_5.970794, whole genome shotgun sequence.
ACCESSION   DAOSIT010000568 DAOSIT010000000
VERSION     DAOSIT010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65126_length_1630_cov_5.970794"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..351
                     /locus_tag="PLL14_11085"
     CDS             91..351
                     /locus_tag="PLL14_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04517.1"
                     /translation="MRKEPAPPIPAAWARGFVSPLMDISLELLLGLLRVFMAMSALTI
                     GKIDGSAEATPLIANVFTGGFPTGRVEASPSRGSVAAAFRIG"
     gene            529..>1630
                     /locus_tag="PLL14_11090"
     CDS             529..>1630
                     /locus_tag="PLL14_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOG04518.1"
                     /translation="MKKQYIVGIDLGTSNTVVAYTAPGKRQVELFEIEQLVGPGQVAA
                     RPLLPSVRYHPAAGEIAAGELQLPWHTPDAEEQVITGRLALDLGAQVPGRLVSSAKSW
                     LSHASVDRLAPILPWGADSEVVKVSPVAASASYLAHVRAAWQHRFPKAPLEQQQLILT
                     VPASFDEGARALTLAAARQAGLPNLRLLEEPQAAFYDWLFRHQERLAEELAATRLLMV
                     CDVGGGTTDLTLIQVSIKDGTPTLTRIGVGDHLMLGGDNMDLALAHLVEGRLPAGEGR
                     LSAARFSQLTARCRAAKEQLLAAGAPAAVQLTLLGGGSRLIGGARSVELQRDEVLQVI
                     VDGFFPRVAADEPIHQRRAAIVEFGLPYPADPA"
BASE COUNT          259 a          548 c          535 g          288 t
ORIGIN      
        1 gctccgagga tccacggcag gaaggtcacc atcgaccgaa cgatccgcga taatgacagg
       61 tattccctgt acgtaaggaa accgatgtcg atgcgcaagg aacccgcacc accaatcccc
      121 gccgcctggg cgcggggttt cgtcagcccg ctgatggaca tctccctgga acttctgctt
      181 ggcctgctgc gggtgtttat ggcgatgtcg gcattgacta tcggcaagat cgacggttct
      241 gccgaggcca cgccactgat cgccaacgtc tttaccggag gctttccgac tgggagggtt
      301 gaagccagcc cctcgcgcgg ctccgttgcg gccgctttcc gcatcgggta agcgtgatga
      361 ggattgcgag gcccctcgcc ggctgattgt cgggtcactg ggacgcctcc ttcagttgtt
      421 ggctgggggg attcggcgtt ctggtgttcg gcggcatggg atgctttggt gtggccgaca
      481 tcggtcgaca tcactcgcca agtccctcat taatgaatga atagcgccat gaaaaaacag
      541 tacatcgtcg gtatcgatct cgggaccagc aacaccgtcg tcgcttacac agcgccgggc
      601 aagcgccagg tcgagttgtt cgagatcgag caactggtcg ggcccggcca ggtggcggcg
      661 cggccgctgc tgccgtcggt gcgttaccat ccggcagccg gtgaaatcgc tgccggtgag
      721 ttgcaactgc cgtggcacac gccggatgcg gaggaacagg tgatcaccgg ccgcctggcg
      781 cttgatctcg gcgcgcaggt ccccggccgg ctggtgagca gtgccaagag ctggctgtcg
      841 cacgcctcgg tcgaccgcct ggcgccaatc ctgccctggg gcgccgacag cgaggtcgtc
      901 aaggtttcgc cggtggcggc cagcgcgagc tatctggcgc atgtccgcgc cgcctggcag
      961 caccgcttcc cgaaggcgcc actcgaacag cagcaactga tcctgaccgt tcccgcctcc
     1021 ttcgacgaag gggcacgcgc gctgacgctg gccgccgccc gccaggccgg cttgccgaat
     1081 ctgcgcctgc tcgaagaacc gcaagccgct ttctacgact ggctcttccg gcatcaggag
     1141 cgactcgccg aagaactcgc cgccacgcgc ttgctgatgg tctgcgacgt cggcggcggc
     1201 accaccgatc tgacgctgat ccaggtgtcg atcaaggacg gcacgccgac actgacgcgt
     1261 atcggcgtcg gcgatcacct gatgctcggt ggcgacaaca tggatctcgc cctcgcccat
     1321 ctcgtcgaag gacgtctgcc ggccggcgag ggccgcctgt cggcggcgcg attttcgcag
     1381 ctcacggcac gctgccgtgc cgccaaggag cagctcctgg ccgccggcgc ccccgcggcc
     1441 gtgcaactga ccctgctcgg cggcggttcg cggctgatcg gcggcgcccg cagcgtcgaa
     1501 ctgcagcgcg acgaggtgtt gcaggtgatc gtcgatggct tcttcccgcg cgtcgcggcg
     1561 gatgagccca tccatcagcg gcgcgcggcg atcgtcgaat tcggcctgcc ctaccccgcc
     1621 gacccggcga
//
LOCUS       DAOSIT010000569         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65426_length_1625_cov_9.617834, whole genome shotgun sequence.
ACCESSION   DAOSIT010000569 DAOSIT010000000
VERSION     DAOSIT010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65426_length_1625_cov_9.617834"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLL14_11095"
     CDS             complement(<1..502)
                     /locus_tag="PLL14_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04519.1"
                     /translation="MHKLLLFRLVFVASRGLRERLTPAGLVIVALTAFAGAFGLDTQA
                     NLAYLLFSLGACGVAVDAAGALLLRWRAPRLAVRRHLPEFVTAGEPARYRLEVLNPQA
                     RPVQAGDLVEQLRQPWPSPASLAHFRSAAATNRFDQRIGYPAFLDLLRRLRAIDVQRI
                     GVPALHP"
     gene            complement(523..1455)
                     /locus_tag="PLL14_11100"
     CDS             complement(523..1455)
                     /locus_tag="PLL14_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOG04520.1"
                     /translation="MTEITAPRAIAANLAKVLHGQQANVRKIVAAWVAGLHVLLDDYP
                     GTGKTTLAKALARSLEASFSRVQFTPDLLPSDIVGVSIFDAQTQSFRFHAGPVFAHVL
                     LADEINRASPRTQSALLEAMAEAQVTVDGQRRILPQPFFVIATQNPVEQAGTYPLPEA
                     QLDRFGIRLALGYVDAAEEERLLDYSAARHPLEQLVPCATVAGLLAARDAAAAIPVAP
                     VLRRYIVSLVQATRTHPAVQMGASPRAAMAIFQLARALALFDGASFVTPDTIQDIAPD
                     VLAHRLALDSQARYSGTTAAEVVREILARIPVPL"
BASE COUNT          278 a          523 c          596 g          228 t
ORIGIN      
        1 cggggtgcag cgccggcacc ccgatccgct gcacgtcgat cgcgcgcagc cgtcgcaaca
       61 ggtccaggaa cgccgggtag ccgatccgct ggtcgaagcg gttggtcgcc gccgcgctgc
      121 ggaaatgcgc gagggatgcc ggcgacggcc agggctggcg caattgttcg acgaggtcgc
      181 cggcctgcac gggacgcgcc tgcgggttca gtacttcgag gcggtagcgc gccggttcac
      241 cggcggtcac gaactcgggc aaatggcggc gcaccgccag acgtggcgca cgccagcgca
      301 acagcagggc gccggcagcg tccacggcga caccgcaggc gcccagggag aacagcaggt
      361 acgccagatt cgcctgggta tcgagcccga aagccccggc aaaggccgtg agcgcgacaa
      421 tcaccagacc ggctggcgtc agccgctcgc gcagcccgcg cgaggccacg aaaaccaggc
      481 ggaagagcag aagcttgtgc atcgccgcaa gcccttcgcc agtcagagcg gcacggggat
      541 gcgcgcgaga atctcgcgga ccacttcggc ggcggtggtg ccggaatagc gcgcctgcga
      601 atcgagcgcc aggcggtggg cgagcacgtc gggcgcgatg tcctggatgg tgtccggggt
      661 gacgaacgat gcgccatcga acaaggccag cgcccgtgcc aactggaaga tcgccatcgc
      721 ggcgcgcgga ctcgctccca tctgcaccgc cgggtgcgtg cgcgtggcct ggaccaggct
      781 gacgatgtag cggcgcaaca ccggcgccac cgggattgcc gccgcggcat cgcgtgccgc
      841 cagcaggccg gccaccgtcg cgcagggcac gagctgctcc aggggatggc gtgcggcact
      901 gtagtcgagc aggcgctctt cctcggcggc atcgacgtag cccagcgcga gccggatgcc
      961 gaagcggtcg agttgtgcct cgggcagcgg ataggtgccg gcctgctcga ccgggttctg
     1021 cgtggcgatc acgaaaaagg gttgcggcag aattcgtcgc tggccgtcga ccgtcacctg
     1081 cgcttcggcc atggcctcca gcagggccga ctgggtgcgc ggcgacgcgc ggttgatttc
     1141 gtcggcgagc aggacatggg cgaacactgg tccggcgtgg aagcgaaacg attgcgtctg
     1201 cgcgtcgaag atcgagacgc cgacgatgtc gctgggcagc agatccggcg tgaactgcac
     1261 ccgcgagaag gaggcctcga gcgaacgggc gagtgccttg gccagcgtcg tcttgccggt
     1321 gccggggtag tcgtcgagca gcacgtgcag gccggccacc caggcggcaa cgatcttgcg
     1381 cacgttggcc tgctgcccat gcaacacctt ggccaggttg gccgcgatgg cgcgcggcgc
     1441 ggtgatctcg gtcatcggga agtcactccg gcagggggcg gtatttccgc gcaaaccact
     1501 tggccaaggc ggagccgtgg gtcaagtcgc tggatcaatg gttctgtcct gtccatcggt
     1561 cggcgggttg agtcgagtcc gaaatttaca gcaaatttca gtacccgcag cactcggcag
     1621 gccat
//
LOCUS       DAOSIT010000570         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65538_length_1623_cov_11.790179, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000570 DAOSIT010000000
VERSION     DAOSIT010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65538_length_1623_cov_11.790179"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLL14_11105"
     CDS             complement(<1..249)
                     /locus_tag="PLL14_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04521.1"
                     /translation="MSVQPRGVHRLPDQGQVFPLFVFGENIMSLEKPRPRPRRSGIES
                     EEERAWVGFYQRVGRDPAIATEVMAQLESDPEMKRTHLA"
     gene            297..527
                     /locus_tag="PLL14_11110"
     CDS             297..527
                     /locus_tag="PLL14_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04522.1"
                     /translation="MLRAGDALRFTPDEIEAFRKLGLDFDGARTQDDIDQALARWADT
                     LNDERPDLLEKIAAAMAKARGIPLPARLTRIR"
     gene            complement(498..722)
                     /locus_tag="PLL14_11115"
     CDS             complement(498..722)
                     /locus_tag="PLL14_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04523.1"
                     /translation="MPISLYRRRIAATVIQLAKDDPSLVKEVIARLRESGEIEADDLV
                     YLDRIADRWMRIAEDNGRHAASTYPRQARG"
BASE COUNT          328 a          496 c          520 g          279 t
ORIGIN      
        1 cgccagatgg gtacgcttca tttcgggatc ggactcgagc tgggccatga cttccgtcgc
       61 gatggccgga tcacggccga cgcgctggta gaagccgacc caggcacgct cttcctcgga
      121 ctcgataccg gagcgccggg ggcgaggacg gggtttctca agggacatga tgttttctcc
      181 aaagacgaac agagggaaca cctgtccctg gtcggggagg cggtggactc cccggggttg
      241 cacggacatg cggcgctgct aagctgaacg ctcacaggtc aagaggtgcc cacaccatgc
      301 tgcgtgcagg cgatgcacta cggttcacgc ccgacgaaat cgaggccttc cgaaaactcg
      361 gtctcgactt cgacggggcg cgaacacagg acgacatcga ccaggcattg gcccgatggg
      421 ccgatacgct gaacgatgaa cgtcccgatc tgctggaaaa gatcgctgcg gcaatggcga
      481 aggccagggg catcccgcta cccgcgcgct tgacgcggat acgttgatgc ggcgtgcctg
      541 ccgttgtcct cggcaatcct catccagcgg tcggcgatcc ggtccaggta gaccagatcg
      601 tcggcttcga tctcgcccga ctctcgcaag cgggcgatca cctccttgac cagggacgga
      661 tcgtccttgg ccagttggat cacagtggcg gcaatgcgcc ggcggtagag gctgatcggc
      721 atgatcagcc tcctgacgcg cggtactcgg aagcgcagcc ggaacgcccg ggggcgttgg
      781 gctgcagctt cagcaacgcc ggcacggcgg gcgtgcgaac agcgggcgtc cgggcatgtc
      841 cggagcctcc cagcagcacg gcgaccgggc gcgactctct cgttacaggg attgcgaaca
      901 tggtgttctc cttcaggaat gaaagggaac acccatccct ggcggggaac ttggggttcc
      961 ccgttcggga tgctccgccg tggacggcag agcggctcgg cgacgtgcca ggcggcacgt
     1021 gcaggatgaa ggcccacggt tcttgcgaac ggggccttcg ctcgatgccg atcgacccga
     1081 aggtcgcgcg gcaggacaca gcgttcagcc gggtggctga acaaaacata cggccatgag
     1141 catggccgga cccgatcgaa gccgggtatc cttgaggcag gtaacgactg cgacgccagc
     1201 agggctggac ttcagggcga ccggggtcgc cgatgaaggc tgtgcagaca cgatgtccac
     1261 agaaagccct tgcagtattg cgcagggctt atccgggctc aagccagcga tctgaattgc
     1321 cacccgcaag cgccagggag gtcaggggat ttgcttgatg aatgcgccgc ggacagaaac
     1381 aggctattcc accttgagac gttcgcagac cacacgccgc cttgagtcag actacgttgt
     1441 gcgggacatc acttcacttg gccactgttg cacatcacaa ctgaccaccc aaattcgtaa
     1501 tcctgaccac gctgatgtca tctgatagca acctgccgtt gctcgatgat tccggaaagc
     1561 aacctttcaa gcggcggctg taccctgaaa actgctgaaa ggccaattca cactcctcgg
     1621 cct
//
LOCUS       DAOSIT010000571         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65542_length_1623_cov_8.466837, whole genome shotgun sequence.
ACCESSION   DAOSIT010000571 DAOSIT010000000
VERSION     DAOSIT010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65542_length_1623_cov_8.466837"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLL14_11120"
     CDS             <1..216
                     /locus_tag="PLL14_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOG04524.1"
                     /translation="VGLSDDNLFHLRTNLRYGCTILRHYLDIERGDLFRALGRYNGSL
                     GQAEYPNIVRMAWQNQWSYSKSKLALR"
     gene            complement(239..1594)
                     /gene="ffh"
                     /locus_tag="PLL14_11125"
     CDS             complement(239..1594)
                     /gene="ffh"
                     /locus_tag="PLL14_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG04525.1"
                     /translation="MLDNLTQRLARVMKTLRGEARLTEANIADALREVRLALLEADVA
                     LPAIKELIAAVKEKAIGEEVIGSLSPGQALVGVVHRELTRLMGDAHSGLNLATQPPAV
                     VLMAGLQGSGKTTTVGKLAKMLRDTQRKKVLVVSCDVYRPAAIEQLKTVAAQAGADFF
                     PSTTTDKPVDIARSAVDWARRHYHDVLLVDTAGRLAIDTAMMKEIADIHAAIRPIETL
                     FVVDAMLGQDAINTARAFSEALPLTGIVLTKLDGDARGGAALSVRHVTGRPIKYAGIG
                     EKLTGLEAFHPERMASRILGMGDVLSLIEDAQKGIDEEKAVEFARKLKSGKGFDLNDF
                     KDQIGQMRKMGGLSSMIDKLPAQFSQAAGQLPAGGEEKAVARIEGIINSMTPTERTRP
                     ELLKAARKRRIALGAGVQVQEVNRLLKQFEQTQKMMKQFSRGGIAQLMRGIKGMLPGM
                     R"
BASE COUNT          279 a          511 c          489 g          344 t
ORIGIN      
        1 gtcggcttga gcgacgacaa cctcttccac ttgcgtacca atctgcgcta cgggtgtacg
       61 attcttcgcc actacctcga catcgaacgg ggtgatctct tccgtgcgct gggtcgctac
      121 aacggcagcc ttggccaggc cgaatacccc aacatcgtgc gcatggcgtg gcagaaccag
      181 tggtcgtatt cgaagagcaa gctggcgctg cgctgaccct gtattgaatg ccgggacttt
      241 agcgcatgcc cggcaacatg cctttgatgc cacgcataag ctgcgcgatg ccgccgcggg
      301 aaaactgctt catcatcttt tgcgtctgct cgaactgctt gagcagccga ttcacttcct
      361 gcacctgaac cccggcaccc agggcgatgc gtcgcttgcg cgcggccttg agcagttccg
      421 gtcgcgtgcg ctcggtcggg gtcatcgagt tgatgatccc ttcgatgcgt gcgaccgcct
      481 tctcctcgcc gccagcgggc aactggccgg cggcctgcga gaactgtgcg ggcagtttgt
      541 cgatcatcga cgacaggccg cccatcttgc gcatctggcc gatctggtcc ttgaagtcgt
      601 tgagatcgaa gcccttccct gacttgagct tccgggcgaa ctcgaccgcc ttttcttcgt
      661 cgatgccttt ctgcgcatcc tcgatcagcg acagcacgtc gcccatgccg aggattcgcg
      721 aagccatgcg ctccgggtgg aaggcttcga ggccggtcag tttttcgcct atccccgcgt
      781 acttgatcgg tcggccagtg acgtgacgaa ccgacagggc tgcacccccg cgcgcgtcac
      841 catcgagctt ggtcagcacg atcccggtca ggggcagggc ctcgctgaag gcgcgggcgg
      901 tattgatcgc atcctgaccc agcatcgcat caacgacgaa caaagtctcg atcggcctga
      961 tcgccgcgtg tatgtcggcg atttccttca tcatcgcggt atcgatggcc aggcggccag
     1021 cggtgtccac cagcaggaca tcgtggtagt gtcgcctggc ccaatcgacc gcactgcgcg
     1081 cgatatcgac cggtttatcg gtggttgtgg aaggaaagaa atcggctccc gcctgcgcgg
     1141 cgacggtttt caactgctcg atggccgccg gacggtagac gtcgcaggaa accaccagga
     1201 cctttttccg ttgcgtatca cgcaacatct tggcgagctt gccgacggtg gtggttttgc
     1261 ccgaaccctg caggccggcc atcagaacga ctgccggcgg ttgtgtcgcc agattcagtc
     1321 cgctgtgggc gtcgcccatc aatctggtga gttcgcggtg aacgacaccg accagagcct
     1381 gtcccgggct cagcgatccg atcacctctt cgccgatggc cttttccttg acggcggcga
     1441 tcagttcctt gatagccggc aaagcgacgt ccgcctcgag caaggccagg cgaacctcgc
     1501 gcagggcatc ggcgatgttg gcttcagtca ggcgtgcttc accgcgcagt gtcttcatga
     1561 cgcgggcaag gcgttgggtc aggttgtcga gcatttggat tccggcttgg tgtaaactca
     1621 aaa
//
LOCUS       DAOSIT010000572         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65598_length_1622_cov_9.735801, whole genome shotgun sequence.
ACCESSION   DAOSIT010000572 DAOSIT010000000
VERSION     DAOSIT010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65598_length_1622_cov_9.735801"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1622)
                     /locus_tag="PLL14_11130"
     CDS             complement(<1..>1622)
                     /locus_tag="PLL14_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOG04526.1"
                     /translation="LIGWAILQGRADARHTAAVATANLSRILADHFNSSVRQIDLGLL
                     NIADVVSRQGNTSRLNETAIDVAIDRQNARHPELIGFQFLGADGRLRYGSINTADTGH
                     IARPDHFLSLAEPSDGGLVVGPAEFGRNSRQWRIAVSRRVNQADGSFAGIAIGSLSAQ
                     ALTESYTRLDLGRHGTVALADTDFTLAAQFPEYDRPDDPGVSSKVASPFLEVIASGAP
                     FTQFDYVSAVDGWRRLATVRKIEGQPYYLLVGLAEDDYLAEWRRNTVWFLLLGALLSG
                     MLLAAIFTLRRHLDSSEAAARAVRESEARFRRMLDEIPIGVCLVDEQGRMYFRNQRFV
                     SLFGYLEADVPDLAEWWQVACPEAGYRQWAQDRWRASVSKAAAGVKDIETVEYRVRCK
                     DGATRDIEIAGVSFGSHFLATFFDQSERRQAEEALRESKIFVQGVLDSVSNHIAVIDE
                     TGRIIATNNAWRQFSLENSSEPGRLAPNTDVGTNYLQVCAGPASSTSAEAAAARDGIR
                     AVLDGRLPTFSLEYPCHSPQQQRWFMMTVTPLG"
BASE COUNT          295 a          507 c          523 g          297 t
ORIGIN      
        1 cccagggggg tgacggtcat catgaaccag cgttgttgct gcggtgaatg gcagggatat
       61 tcgaggctga aggtcggcag gcggccatcg agcacggcac ggatgccgtc acgggcggcc
      121 gccgcctctg ccgaggtgga ggatgccggg cccgcgcaga cttgcaggta gttggtcccg
      181 acatcggtgt ttggcgcgag gcggccaggc tcggagctgt tctccagcga gaattgacgc
      241 caggcgttgt tggtcgcaat gatcctgccg gtttcatcga tcaccgcgat gtgattggac
      301 acggagtcaa gcacgccctg gacgaagatc ttgctttcgc gcaaagcctc ttcggcttgc
      361 cggcgttcgc tctggtcgaa aaaggtggcg agaaagtggc taccgaagga gacgccagcg
      421 atttcgatgt cgcgcgtagc accgtccttg cagcgtacgc ggtattcgac ggtctcgatg
      481 tccttgacgc ctgcggcggc tttgcttacc gacgcccgcc accggtcctg cgcccattgg
      541 cgatagccgg cttccgggca ggccacctgc caccactcgg caaggtcggg cacgtcggct
      601 tccaggtagc cgaacaggct gacaaagcgc tgattgcgaa agtacatccg gccttgctca
      661 tcgaccagac agaccccgat cggtatctcg tccagcatcc gcctgaacct tgcttcactc
      721 tcccggacgg cgcgtgcggc agcctccgaa ctatcgaggt gccggcgcag ggtgaaaatc
      781 gccgccagca gcattcccga gagcagcgct ccgagcagca gaaaccagac ggtattgcgt
      841 cgccattccg caagatagtc gtcctcagcc agtccgacca gcaggtagta gggttgtcct
      901 tcaatctttc ggactgtcgc caggcggcgc cagccgtcca ccgcggaaac atagtcgaac
      961 tgggtgaacg gggctccgga agcgatgacc tccaggaacg gtgaggctac cttgctgctg
     1021 acgcccggat cgtcgggcct gtcgtactcg gggaactggg cggcaagcgt gaagtcggtg
     1081 tcggcaaggg cgacggtccc atgccgtccg agatccagcc gggtatagct ctcggtcaag
     1141 gcctgcgccg acagcgagcc gatggcgata ccggcgaagg aaccatcggc ctggttgacc
     1201 ctgcgcgaca ccgcaatccg ccattgccgc gaattccgac cgaactccgc cgggccgacc
     1261 accagcccgc catcggatgg ctcggccagg gacaggaagt ggtctggtcg tgcaatgtgg
     1321 ccagtgtcgg cagtgttgat cgagccgtaa cgcaatcttc catccgcccc caggaactga
     1381 aagccgatca gctccgggtg tcgcgcattc tgccggtcga tggcgacatc aatcgctgtt
     1441 tcgttgagcc ggctggtgtt tccttgccgg gagacgacgt ccgcgatgtt cagcaggccc
     1501 aggtcgatct gccgcacgct gctgttgaaa tggtctgcca ggattctgga taggtttgcc
     1561 gtagccaccg cggccgtgtg ccgggcatcg gcccgaccct gcagaatcgc ccaaccgatc
     1621 aa
//
LOCUS       DAOSIT010000573         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_65599_length_1622_cov_9.449266, whole genome shotgun sequence.
ACCESSION   DAOSIT010000573 DAOSIT010000000
VERSION     DAOSIT010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65599_length_1622_cov_9.449266"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PLL14_11135"
     CDS             <1..994
                     /locus_tag="PLL14_11135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG04527.1"
                     /translation="TGKVQLAIDNGVEGNPGFIMYLPVFAQGQPRDSVTQRRDSLIGW
                     VYASFHMRDFMASLYGRQAEGLAFAIYDGAVPSDAALMYRNREAAGGALSAKEYMVVA
                     SHTWTLTLVTQEEFENRFGRDISLIIAVAGVSLSLSMALLAWSMINGRARALLLAARI
                     TEELRHMAQHDSLTGLPNRALFSDRVQNKLAHARRHDRRFALIFLDLDKFKPINDKHG
                     HSVGDLLLRQVARRLQDSIRASDTVGRLGGDEFVLLIGELTGAADALALAEKIRQEVR
                     RPYTINGHQLTISCSLGVAIYPDDGTDELALTKRADEAMYRAKNSGRDSVRLAN"
     gene            complement(1062..>1622)
                     /locus_tag="PLL14_11140"
     CDS             complement(1062..>1622)
                     /locus_tag="PLL14_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HOG04528.1"
                     /translation="KRIIDHWVYGGVLAGVLLLLLAPLLTAAWSTALTATFLHLPFYM
                     LHQYEEHDDDRFRRFFNQTIGKGQEVLSREAVFVTNVPGVWGVVAVSLYLASEVHIGL
                     SLIAVYLVLVNAFVHLVHAFIFRCYNPGLATALAMFAPLGIYTLRAVQLAGGGSAALH
                     ALGAGIAVAIHAAILAHVHRRLRRGA"
BASE COUNT          316 a          522 c          520 g          264 t
ORIGIN      
        1 taccggcaag gtgcaattgg ccatcgacaa cggtgtcgaa ggcaaccccg gcttcatcat
       61 gtatctgccg gtctttgccc aggggcagcc gcgagacagc gtcacgcagc gtcgtgacag
      121 cctgattggc tgggtctatg cctcgttcca catgcgcgac ttcatggcca gtctgtatgg
      181 acggcaagcc gagggcctcg ccttcgccat ttacgacggc gccgtcccca gtgatgccgc
      241 actcatgtac cggaaccggg aggcggccgg tggcgcgttg agtgccaaag agtacatggt
      301 cgttgccagt cacacctgga cgctcaccct ggtcacgcag gaggaattcg aaaaccgctt
      361 cggccgggac atctcgctga tcattgccgt ggcgggtgtc agcctgagcc tgtcgatggc
      421 gttgctggcg tggtcgatga tcaacgggcg cgcgcgtgcc ttgctactgg ccgccaggat
      481 caccgaggaa ttgcggcaca tggcacagca tgattcgctg accggcctgc ccaaccgagc
      541 gttgttcagt gaccgcgtcc agaacaagct ggcccatgcc aggcgccatg accgccgctt
      601 cgccctgatc ttcctcgacc tcgacaagtt caaaccgatc aacgacaagc acggccattc
      661 cgtcggcgac ctgctgttgc gacaggtggc aagacgtctg caggattcga ttcgggcctc
      721 cgatacggtc ggccgcctgg gcggcgacga gttcgtcctg ctgatcggcg aactgactgg
      781 tgcggcggac gcgctggcgc tggctgaaaa gattcgtcag gaagtgcggc ggccgtacac
      841 catcaatggt caccaactga cgatttcatg cagcctcggg gttgccatct atcccgacga
      901 tggcaccgat gaacttgccc tgaccaagcg cgccgacgaa gccatgtatc gcgccaagaa
      961 cagtggccgc gacagcgtgc ggttggccaa ctgaacccgc tacctgcggc caaatgtcgg
     1021 ccggcgccgc cagggccgcc cgacagcgcc gcggccggac gctacgcgcc gcggcgcagc
     1081 cggcgatgca catgcgccag tatcgcggcg tggatcgcga ccgcaatgcc ggcgccgagc
     1141 gcgtgcaatg cagcgctgcc gccgccggcc agctggaccg cgcgcagcgt atagatcccg
     1201 agcggcgcga acatcgccag ggccgtcgcc agaccggggt tgtagcagcg gaagatgaac
     1261 gcgtgcacca ggtgcacgaa ggcattgacc agcaccaggt acaccgcgat cagcgacagt
     1321 ccgatatgga cttcagaagc caggtacagc gagacggcga cgacccccca caccccgggg
     1381 acgttggtga caaacaccgc ttcgcgggag aggacctcct ggcccttgcc gatcgtttga
     1441 ttgaaaaacc gccggaagcg gtcgtcatcg tgctcctcgt actggtggag catgtagaac
     1501 ggcagatgca gaaaggtggc tgtcagcgcc gtcgaccacg cggcggtgag caagggcgcg
     1561 agcagcagca acagcactcc ggcgaggacg ccgccgtata cccaatggtc aatgattcgc
     1621 tt
//
LOCUS       DAOSIT010000574         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66158_length_1612_cov_9.292871, whole genome shotgun sequence.
ACCESSION   DAOSIT010000574 DAOSIT010000000
VERSION     DAOSIT010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66158_length_1612_cov_9.292871"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLL14_11145"
     CDS             complement(<1..914)
                     /locus_tag="PLL14_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG04529.1"
                     /translation="MSEPSAPPSPAEQSFPAVEISPAQGVAGEAAPLVSVDVGQQLRA
                     AREARNMSLAEAAQSLKLAPRQVEALEAEDWAALPGNTMIRGFVRNYARLLNIDSDLL
                     MRGLDAAQLQQTLHLEISAGTSASLPQAGRRVERRDYLAVLAGLLLLSLAVLAYFYVP
                     ADFWREKIGGLLGRGASQAPVEEPAPPPVVAVPKAAVGESVTVLATPHATLLSDMAAP
                     TRPAGGAAAPTGGGLKLSFAQPAWVEVRDGRGQTIFAELGSAGSQREIDGQPPFSLVV
                     GNATHVKLEYRGRAIELAPRSKDDVARLT"
     gene            complement(911..>1612)
                     /gene="pilW"
                     /locus_tag="PLL14_11150"
     CDS             complement(911..>1612)
                     /gene="pilW"
                     /locus_tag="PLL14_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HOG04530.1"
                     /translation="ADSRTPTKPRDPRTRAKLHTELGALYLQNGNLAVALEELTMAIV
                     IDPDYAKAYATRGLAGFHVREMQLADQDFQRALSIDGNDPEINNNYGWFLCQVGREKE
                     AITFFQRAIRNPLYETPERAYLNAGACYVKIGDFAAAENFVQHSLRIAPDNPQALLQM
                     AGINYRRGYIELAKQQLSELLRKTEPSAEALWLGVRVERRLGERVAEARYGSQLRRKF
                     PLSPETQELLRGNYE"
BASE COUNT          272 a          522 c          514 g          304 t
ORIGIN      
        1 gtgagccgcg ccacatcatc cttgctgcgt ggcgcgagct ctatcgccct gccccggtat
       61 tcgagcttta cgtgcgtcgc attgccaacc accagcgaaa aaggcggctg tccatcgatt
      121 tcccgctggc tacccgccga acccagttcc gcaaaaatgg tttgtccgcg cccatcgcgt
      181 acctcgaccc aggccggctg cgcaaagctc agcttcaggc cgccgccggt tggtgcggcg
      241 gcacctcccg cgggccgggt tggcgccgcc atgtcggaca gcagggttgc atgcggggtc
      301 gcgaggaccg tcaccgattc cccaacggca gccttgggca cggcaacgac tggcggtggt
      361 gccggctcct cgactggcgc ctgcgaggcg ccgcggccga gcaggccgcc gattttttcc
      421 cgccagaaat cggcggggac gtagaaatag gcgagcacgg caaggctcag cagtaacagc
      481 ccggccagca cggcgagata atcgcggcgt tcaacgcggc gacccgcttg cggcaaactg
      541 gcactggtgc cggccgagat ctcgaggtgc agcgtctgct gcagttgcgc ggcatccagc
      601 ccgcgcatca gcagatccga gtcgatattc agcaggcgtg cgtagttgcg aacgaaaccc
      661 ctgatcatcg tattgccggg caacgctgcc cagtcctcgg cttcgagggc ttccacctgt
      721 cgcggagcga gcttgagcga ctgtgccgcc tcggccaggc tcatgttcct ggcctcgcgg
      781 gcagcgcgca gttgctgccc gacgtcaacg gatacgagcg gcgctgcctc gccggccacg
      841 ccttgcgccg gcgagatttc cacggccggg aagctctgtt ccgctggact ggggggtgcg
      901 gatggttcac tcattcgtag ttgcctctga gcagttcctg ggtctctggc gagagtggga
      961 atttgcgccg caactggcta ccgtagcgtg cctcggcgac ccgctcacca agccggcgct
     1021 cgacccgcac cccgagccac aacgcctccg cgctgggctc ggtcttgcgc agcaactccg
     1081 agagttgctg cttcgccagt tcgatgtaac cgcgtcgata gttgatgccg gccatttgca
     1141 acaaggcctg cgggttgtcc ggagcgatcc gcagactgtg ctggacaaaa ttctcagccg
     1201 cagcaaaatc gccgatcttg acgtaacagg cgccggcatt cagataggcg cgttccggag
     1261 tctcatacag cgggttcctg atggcgcgct gaaagaaagt gatcgcttcc ttttctcgcc
     1321 ccacctggca caggaaccag ccatagttat tgttgatctc ggggtcgttg ccgtcaatac
     1381 tcagggcgcg ctgaaaatcc tggtccgcga gttgcatctc gcgcacgtgg aaacccgcga
     1441 gtccgcgtgt tgcataggcc ttggcatagt ccgggtcgat cacgatggcc atggtcagct
     1501 cttcgagggc caccgcaaga ttgccattct gcaggtaaag cgcaccgagc tccgtgtgca
     1561 gtttggcgcg tgtgcgggga tcccgaggtt tggtcggcgt acgcgagtcg gc
//
LOCUS       DAOSIT010000575         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66382_length_1608_cov_10.137798, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000575 DAOSIT010000000
VERSION     DAOSIT010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66382_length_1608_cov_10.137798"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLL14_11155"
     CDS             complement(<1..1340)
                     /locus_tag="PLL14_11155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG04531.1"
                     /translation="MTFAEIGLHPAILKALTDSGYSEPTPVQQQAIPAALAGRDLMVS
                     SQTGSGKTAAFMLPALHLFASQERPAATTRAGDRRPAHARQRFQAAQPKMLVLTPTRE
                     LALQVTAATEKYGRQLHHLRAVAILGGMPYPKQMELLGRNPEILVATPGRLIDHMSSG
                     KIDFSQLQILVLDEADRMLDMGFIEDIERIVAATPASRQTLLFSATLDGTVGELAQRM
                     TRAPLRLQVDAVSARHDNIEQRMHFIDDLAHKNRLLDHLLRDVSIGQALVFTATKRDA
                     DTVSDRLNVAGFNAAALHGDMQQGARNRTLAAMRRGQVQILVATDVAARGIDVPTITH
                     VFNYDLPKSAEDYVHRIGRTGRAGRSGLAISLVHHAEHFNVRKIERFTKQMIPVEVVA
                     GHEPTRRPASANPRPTAKPAWKGGEKRSYGQPAGAAADSRFSKPAAPRRDGAPRA"
BASE COUNT          260 a          471 c          581 g          296 t
ORIGIN      
        1 gcccgcggtg cgccgtcgcg gcgcggcgcg gcgggcttgc tgaagcggct gtcggccgct
       61 gctcccgccg gctgcccgta gctgcgcttt tcaccgcctt tccaggcggg tttggcggtt
      121 gggcgcgggt tcgccgatgc cggccggcga gtgggctcat ggccggcgac gacttcgacc
      181 gggatcatct gcttggtaaa gcgttcgatt ttccggacgt tgaaatgctc ggcgtgatgg
      241 accagcgaga tggccagacc actgcgcccg gcgcggccgg tgcggccgat gcggtgcacg
      301 tagtcctctg ccgacttcgg cagatcgtag ttgaagacgt gcgtaatcgt cggcacgtcg
      361 atgccacgcg ccgccacgtc ggtggcgacc agaatctgta cctgcccacg gcgcatcgcg
      421 gcgagggtgc ggttgcgggc cccctgctgc atgtcgccgt gcaaggccgc ggcgttgaat
      481 ccggcgacgt tcaggcgatc actgacggta tcggcgtcac gcttggtggc ggtaaacacc
      541 agagcctggc cgatcgaaac gtcgcgcaac aggtggtcga gcaggcggtt cttgtgcgcg
      601 aggtcgtcga tgaagtgcat ccgctgctcg atgttgtcgt gccgggccga aacggcgtcg
      661 acctgcagcc gcagcggcgc gcgtgtcatg cgctgtgcca gttcgccgac ggttccgtcg
      721 agggtggccg agaacaacag cgtctggcgc gaagccggag ttgcggcgac gatcctttcg
      781 atatcctcga tgaaacccat gtccagcatg cggtccgctt cgtcaaggac cagtatctgc
      841 agttgcgaga agtcgatctt gcccgagctc atgtggtcga tcaggcgacc cggcgtggcc
      901 accaggatct cgggattgcg gccgaggagt tccatctgct tcggataagg catgccgccg
      961 aggatggcga ccgcgcgcag gtgatggagc tggcgcccgt acttctcggt ggcggcggtg
     1021 acctgcaggg caagctcgcg ggtgggcgtc aggaccagca tcttcggttg cgcagcctgg
     1081 aagcgctggc gcgcgtgtgc cggccggcgg tcgcctgcac gggtggtggc ggccggccgt
     1141 tcctgcgagg cgaagaggtg cagcgccggc agcatgaagg ccgccgtctt gccggaaccg
     1201 gtttgcgagg aaaccatcag gtcgcggccc gcgagggcgg cgggtatggc ttgttgctgg
     1261 accggcgtcg gttcgctgta gccggagtcg gtgagtgcct tcaggatggc cgggtgaagg
     1321 ccgatttctg caaaagtcat atttctcttt cgtatgggtg tgctgcgcgc tgccgtaacg
     1381 gcatggcacg ctgccaaggg aaaaaagcaa cgccaaccaa cgaaaaagct gagagacaga
     1441 ctctgttcga tcaacaggag tcagcaccaa tcagcacaaa agctttgtgc caggacggcg
     1501 ctgattgaat cgacaccaga aggcaggggg gcttgagggt tacggccagg ttcgcgatgc
     1561 ttgttgcacg ctccgctgag gcggcgtcgt gcggcgcgca ttatagca
//
LOCUS       DAOSIT010000576         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66737_length_1602_cov_8.334195, whole genome shotgun sequence.
ACCESSION   DAOSIT010000576 DAOSIT010000000
VERSION     DAOSIT010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66737_length_1602_cov_8.334195"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLL14_11160"
     CDS             complement(<1..1084)
                     /locus_tag="PLL14_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG04532.1"
                     /translation="MHLTDPRLLRSNCYMDGRWEAADDGRTLPVSDPADGGILGEVPM
                     MAAVETRRAIAAAAAALSAWQAKTAKERAAVLRQWHGLILANGEDLARLITAECGKPI
                     GEARGEVAYGASFVEWFAEEGKRAYGESIPSPTADRRLLTIRQPVGVCAAITPWNFPL
                     AMITRKVAPALAAGCTVVVKPAEQTPLTALALARLAHDAGLEAGVLNVLTGDPVAIGG
                     ALTASPVVRKLSFTGSTEIGRLLMAQCAPTIKKLSLELGGNAPFIVFDDADIAAAVEG
                     AIIAKYRNTGQTCVCANRLLVQDRVYDRFVEQLASRVASLKVGPGNDEGVAQGPLIDA
                     AALAKVEAHIADAVAGGARVVTGGHRH"
     gene            1130..>1602
                     /locus_tag="PLL14_11165"
     CDS             1130..>1602
                     /locus_tag="PLL14_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOG04533.1"
                     /translation="MRVHILLAGVVSLLLVACAGKTPLPERLPEPPARHPTSSYKGHD
                     VAMFALSLIDTGYRFGGKNPEAGLDCSGMVSYIYNRAVGLRVSGSAADIARRGRPVER
                     TGLRPGDLVFFNTRNAAYSHVGVYIGDDRFVHAPSSSGRVRIDQLDARYFAQRFDA"
BASE COUNT          278 a          566 c          482 g          276 t
ORIGIN      
        1 cgtgccggtg gccaccggtg accacgcgcg cgcccccggc aacggcgtcg gcgatatgcg
       61 cttcgacctt ggccagcgct gccgcgtcga tcagcggccc ctgagcgacg ccttcgtcgt
      121 tccctggccc caccttcagc gacgccacgc gactggccaa ttgctcgacg aagcgatcgt
      181 agacccggtc ctgaaccagc agccggttgg cacagacgca ggtctgtccg gtgttgcgat
      241 acttggcgat gatggcgcct tcgactgcgg ccgcaatatc cgcatcgtcg aagacgatga
      301 acggcgcgtt cccaccaagc tcaagcgaaa gcttcttgat cgtcggtgcg cactgcgcca
      361 tgagcagacg accaatctcg gtagacccgg tgaacgacag cttgcggacc accgggctgg
      421 cggtcagcgc gcccccgata gccaccggat caccggtcag cacgttgaga acccccgcct
      481 ccagaccggc atcgtgcgcc aggcgcgcca atgccagagc cgtcaatggc gtctgctcgg
      541 ccggcttgac caccaccgta cagccagccg caagcgccgg tgccaccttg cgggtgatca
      601 tcgccagcgg gaaattccac ggcgtgatcg ccgcacacac ccccaccggc tgtcggatgg
      661 tcagcagacg ccgatcggcg gtcgggctcg gaatgctttc gccgtaggcg cgcttgccct
      721 cctcggcaaa ccactcgacg aaggaggccc cgtaggccac ctcgccgcgt gcttcgccga
      781 tcggtttgcc acattcagcc gtaatcagcc gcgccaggtc ctcgccattg gcaagaatca
      841 acccatgcca ctgtcgcagg acggcagcgc gttccttggc cgtcttcgcc tgccaggccg
      901 atagcgccgc agccgcagcc gcgatcgcac gccgagtctc gactgccgcc atcatgggga
      961 cctcgccgag aatcccgccg tcggccgggt cgctgaccgg cagggtgcgg ccgtcatcgg
     1021 cagcctccca acgaccgtcc atgtaacagt tcgagcgcag caggcgcgga tcggtgaggt
     1081 gcatggcaat tccccgaaat ctgaaaatct gcagtttata ctgtgcacca tgcgtgtcca
     1141 tattctcctc gccggagtcg tttccctgct gctggtcgcc tgtgccggca agacaccgct
     1201 accggaaaga ctgcccgaac caccggcccg gcatccgacg agcagctaca agggccacga
     1261 cgtagcgatg tttgctctca gcctgatcga tacgggttac cgcttcggcg gcaagaaccc
     1321 cgaagcgggt ctcgactgca gcggcatggt ttcctatatc tacaaccgcg ccgtgggcct
     1381 ccgggtaagc ggcagcgcgg ccgacattgc ccggcgtggc cgcccggtcg aacgtacggg
     1441 gctgcgtcct ggcgacctgg tgttttttaa tacccgcaac gcggcttatt cacacgtcgg
     1501 cgtctacatt ggtgacgatc gattcgttca cgccccgtcg agcagcggcc gggtgcgcat
     1561 tgatcaactc gatgcccgct acttcgcgca gcgcttcgat gc
//
LOCUS       DAOSIT010000577         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66797_length_1601_cov_10.912678, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000577 DAOSIT010000000
VERSION     DAOSIT010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66797_length_1601_cov_10.912678"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /locus_tag="PLL14_11170"
     CDS             complement(<1..>1601)
                     /locus_tag="PLL14_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOG04534.1"
                     /translation="YANGDIHIGHAVNKILKDVIVRSKTLAGFDAPYVPGWDCHGLPI
                     EHQIEKLHGKHVPAERVRELCRAFAGEQIERQKKDFIRLGILGAWDKPYLTMNFKTEA
                     DEIRALGAILQKGYLYQGLKPVNWCLDCSSALAEAEVEYEDKTSSAIDVAFEVHPNHA
                     DKLARAFGLTHLRGPAFAVIWTTTPWTLPANEAVSAHPDFDYELIDTPRGALILVREL
                     AAACLQRYALAGTVIGSAKGAALEHVLLKHPFQPRDVAIICGRHVTVEAGTGLVHTAP
                     AHGTDDYLIGKVYGLPVNNPVGDDGRFVAGTPALGVGELAGRSVWEANPLVLQELAAN
                     GHLLQSEKIQHSYPHCWRHKTPIIFRATTQWFIGMDHRPTSSEAAATPVSEEEPTLRW
                     CAERAVDETRFFPTWGRARLEAMMRTRPDWCVSRQRNWGVPLPFFLHRETGAPHPRTV
                     ELIEAVAQRVEKAGIEAWFALDAEELLGAEAEQYRKMSDTLDVWFDSGATHSSVLRGS
                     HRSELAYPADLYLEGSDQHRGWFQS"
BASE COUNT          261 a          488 c          557 g          295 t
ORIGIN      
        1 aactctggaa ccagccacga tgttgatcgg agccttcgag gtagagatcg gcggggtagg
       61 cgagttcgct gcgatgcgag ccgcgcagga cgctggagtg cgtcgcgccg gagtcgaacc
      121 agacgtcgag ggtgtcgctc atcttccggt attgctcggc ttcggcgccg agcagttctt
      181 cggcgtcaag cgcgaaccag gcttcgatgc cggccttttc gacgcgctgg gcgacggctt
      241 cgatgagttc gacggtgcgt ggatgcggcg caccggtctc gcgatgcagg aaaaagggca
      301 ggggaacgcc ccagttgcgc tgacgcgaga cgcaccagtc gggacgcgtg cgcatcatcg
      361 cttccagacg cgcacgaccc caggtcggga agaagcgcgt ttcgtcgacc gcgcgctcgg
      421 cgcaccagcg cagcgtgggt tcctcttcgc tgacgggcgt cgccgcggct tcggacgacg
      481 tcggtcggtg atccatgccg atgaaccact gcgtggtggc acggaagatg atcggtgtct
      541 tgtgacgcca gcagtgcgga taactgtgct ggatcttctc ggattgcagc aggtggccgt
      601 tggcggcgag ttcctgcagc accagcgggt tggcctccca gaccgatctg ccggcaagct
      661 cgccgacgcc cagcgccggg gtgccggcga cgaatcggcc gtcgtcgccg accggattgt
      721 tcaccggcag accgtacacc ttgccgatca ggtagtcgtc ggtgccatgc gcaggcgccg
      781 tgtgtaccag gccggtgccg gcctcgacgg tgacgtgccg gccgcagatg atggccacat
      841 cgcgcggctg aaacgggtgt ttgaggagca cgtgttcgag cgccgcgcct ttggccgaac
      901 cgatcaccgt gccggcgagg gcatagcgct gcaggcaggc agcggccagt tcacgcacca
      961 ggatcagggc gccgcggggc gtgtcgatca gttcgtagtc gaagtccggg tgcgcgctga
     1021 cggcctcgtt ggccggcagc gtccagggcg tggtcgtcca gatcacggcg aaggccggcc
     1081 cgcgcaggtg cgtgaggccg aaagcccgcg ccagcttgtc ggcgtgattg gggtgtactt
     1141 cgaaagccac atcgatcgcc gacgaggtct tgtcttcgta ttcgacctcg gcctcggcca
     1201 gcgccgaact acaatcgaga caccagttca ctggtttcag gccctggtag aggtaaccct
     1261 tctgcaatat cgcgccgagc gcacggattt cgtcggcttc ggtcttgaag ttcatggtca
     1321 ggtagggctt gtcccaggcg ccgagaatac cgaggcggat aaaatccttc ttctggcgct
     1381 cgatctgttc gccggcgaag gcgcgacaca gttcacgcac ccgctcggcc gggacatgct
     1441 tgccgtgcag cttttcgatc tgatgctcga tcggcaggcc atggcagtcc cagcccggaa
     1501 catacggtgc gtcgaagccc gccagcgtct tcgagcggac gatgacgtcc ttgaggatct
     1561 tgttgaccgc gtggccgatg tggatgtcgc cgttggcgta g
//
LOCUS       DAOSIT010000578         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66849_length_1600_cov_4.982524, whole genome shotgun sequence.
ACCESSION   DAOSIT010000578 DAOSIT010000000
VERSION     DAOSIT010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66849_length_1600_cov_4.982524"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLL14_11175"
     CDS             <1..288
                     /locus_tag="PLL14_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOG04535.1"
                     /translation="LVQFDHRLGAWLLAFLVPWLWWKLSRAVVSRRVRLAATLLLIVL
                     FAQIGLGIATLLLAVPVGLGAAHQGGAMVVLGVLLWLNHELRAVPSSGGAT"
     gene            288..1229
                     /locus_tag="PLL14_11180"
     CDS             288..1229
                     /locus_tag="PLL14_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOG04536.1"
                     /translation="MASLGLRLRKVRALALLLTALSLLVVLLSAYLRLDGAGLGCADW
                     PACYGALLSSERQEQHFGVARLLHRAAASLSLLLACTLVWQCRRRPMIESAAAPATLL
                     LLLMLALSALGIWSSDPRLMPVGFLNILGGLGLVSFSWRVVLASRPGSALDQPDEPKL
                     LLRVGAVVLTSTLVLGALLGSGYAAPACPSFPDCAGRWWPTAAGWAALQPLSILTSVP
                     QAGDPGGVTLNLLHRYSAMCALILLGTAALQAMREEQRRRAAILVLVLLTTEVALGTL
                     TVISGFSLGLAISHGVCAAALLAAVATLLRSQVCRHQ"
     gene            complement(1280..>1600)
                     /gene="erpA"
                     /locus_tag="PLL14_11185"
     CDS             complement(1280..>1600)
                     /gene="erpA"
                     /locus_tag="PLL14_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOG04537.1"
                     /translation="FVFTDSAAGKVKELIEEEGNPDLKLRVFVTGGGCSGFQYGFTFD
                     EVANDDDTALQKNGVTLLVDPMSYQYLVGAEIDYTEGLEGSQFVIKNPNASSTCGCGS
                     SFSV"
BASE COUNT          217 a          502 c          540 g          341 t
ORIGIN      
        1 ctggtccagt ttgaccaccg cctgggcgcg tggctgttgg ccttcctcgt gccctggctc
       61 tggtggaagc tcagccgcgc cgtcgtctcc cggagggtac gcctggcggc aacgttgttg
      121 ctgatcgtgc tcttcgcgca gatcggactc gggattgcga cactgctgct ggcggttccc
      181 gtgggcctgg gtgccgcgca tcagggtggc gccatggtcg tcctgggtgt gctgttgtgg
      241 ttgaatcacg aactacgcgc ggtgccctcg tccggcggcg ccacctgatg gccagcctgg
      301 gtctgcgcct gcggaaagtg cgcgcgctgg cactgctgtt gacggcgctg tcgctgctgg
      361 tggtgttgct cagtgcttac cttcgtctcg acggcgccgg cctgggttgt gccgattggc
      421 ccgcctgcta tggggcgttg ctgagcagcg agcggcagga gcagcatttc ggcgtcgcgc
      481 gactgctgca tcgcgccgcc gcgtcgttgt cgctgctgct ggcctgcacg ctggtctggc
      541 aatgccggcg gcgacccatg atcgagtcgg cggccgctcc ggcgacgctg ctgctgctgt
      601 tgatgctggc tctctcggcg cttgggatct ggagttcaga tcccaggctg atgccggtcg
      661 gtttcctgaa catcctgggt ggcctcggtc tggtcagctt ctcgtggcgg gtggtgctgg
      721 ccagtcgccc cggatctgcg ctcgaccaac cggacgagcc gaagctgttg ctgcgcgtcg
      781 gggccgttgt ccttaccagt actttggtcc tgggggcgct gctcggttca ggctacgcgg
      841 cgccggcctg cccatcgttt ccggactgtg ccggtcggtg gtggccgaca gcggccggct
      901 gggcggcgct gcagcccttg tcgatcctga cttcggtgcc gcaagccggc gatcccggtg
      961 gcgtcaccct gaatttgctg caccgctaca gcgccatgtg cgcgctgatc ttgctgggca
     1021 ccgcagcgtt gcaggcgatg cgcgaagagc aacgacgccg ggcggcgatc ctggtgctgg
     1081 tcctgctcac aaccgaagtc gcgctgggta ccttgaccgt catcagtggc ttcagtcttg
     1141 ggctggcgat cagccacggg gtgtgtgctg cagcactgct ggcggcagtg gcgaccctgc
     1201 tccgaagtca ggtctgtcgc caccagtgac tgcgtgttcc agcaatgact gaaaccgcaa
     1261 cagcggtgcc gtgccgcaac tataccgaga acgacgaacc gcagccgcaa gtcgaagagg
     1321 cgttgggatt cttgatcacg aactgcgaac cctcaagccc ttcggtgtag tcgatttcag
     1381 caccaacgag gtactggtag ctcatcgggt cgaccagcag ggtgacgccg tttttctgca
     1441 gcgccgtgtc gtcgtcattg gcgacctcgt caaaggtgaa gccatactga aaacccgagc
     1501 agccaccacc ggtgacaaat acgcgcagtt tcaggtccgg gttgccttcc tcttcgatca
     1561 actccttcac cttgccagcc gcgctgtcgg tgaagacgaa
//
LOCUS       DAOSIT010000579         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66983_length_1598_cov_8.970188, whole genome shotgun sequence.
ACCESSION   DAOSIT010000579 DAOSIT010000000
VERSION     DAOSIT010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66983_length_1598_cov_8.970188"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLL14_11190"
     CDS             <1..462
                     /locus_tag="PLL14_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOG04538.1"
                     /translation="DDHEELREWMAAVALDAKAKIPPLPVVEAYEAVPYDVGNLLDPF
                     KPAKMAPDEKKGGGGFRPDLDRPKEPLELYPLESLKYVGVMTRQKVSYAIVQVDGTLH
                     QIKIGNYMGQNFGVVVDVSESEITLRELIQDSAGDWVERTSTLLLQEKGAK"
     gene            463..>1598
                     /locus_tag="PLL14_11195"
     CDS             463..>1598
                     /locus_tag="PLL14_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HOG04539.1"
                     /translation="MKQIRQYLLGLVGGVLLALGTANAQEARLNSIESMNVVQQGSVL
                     NVKLTFKEPLQALPSAFSIAKPARIAFDFSNTTNGLGKSNQAYNEGDLKSANIVQAED
                     RTRVVLNLNKAMTYEATIDGNNLLLALVPSATPAGLAPVEHFAQARPSAMSNSIRDIS
                     FRRGKDGEGRITVDLTDANAGIDIKQQGSNLVVDFAKVTLPEALRKRLDVTDFATPVL
                     TVTTVEKGPNTRMTITPRGLWEHNAYQSDNQFVIEVKPIIENPNKLVQGSRGGYQGEK
                     LSLNFQNVDVRRLLQVIGEFTGMNMVVSDSVGGSITLILKDVPWDQALDIIMQQKGLD
                     MRKNGNVILIAPREEIATKEKLEFESKAQIGDLEPLQTESFQMN"
BASE COUNT          373 a          425 c          493 g          307 t
ORIGIN      
        1 gacgatcatg aggaattacg ggaatggatg gctgccgtgg cgctggatgc caaggccaaa
       61 atccctccgc tgcccgtggt cgaagcctac gaggccgttc cttatgatgt gggcaatctt
      121 cttgaccctt tcaaaccggc caagatggct cctgacgaga agaaaggtgg cgggggtttc
      181 cggccggatc tggatcggcc gaaagagcct ctggagcttt atcccctgga atcgctgaaa
      241 tacgtcggtg tgatgaccag acagaaggtg tcgtacgcca tcgtacaggt cgacgggact
      301 ttgcaccaaa tcaagatcgg aaactacatg gggcagaact tcggcgtggt ggtggacgtg
      361 tccgaatcag agatcacctt gcgagaactc atccaggatt cggcgggtga ctgggtggaa
      421 cggacgagca ccttgctgtt gcaggagaag ggagcgaagt aaatgaagca gattagacaa
      481 tacctgctcg gtctggttgg tggtgtgctg ttggcgctgg gaacggcgaa tgcacaggaa
      541 gccaggctga attccatcga gtcgatgaac gtcgtgcaac aaggctcggt gctcaacgtc
      601 aaacttacct tcaaggaacc attgcaggct cttccgtcgg cgttcagcat tgccaaaccg
      661 gcacgcatcg cttttgactt ttcgaacacc accaatggct tgggcaagtc gaaccaggca
      721 tacaacgagg gcgacttgaa gagcgccaat attgtccagg ccgaggaccg gacccgggtg
      781 gtcctgaacc tgaacaaggc catgacctat gaggcgacca ttgatggcaa caacctgctg
      841 cttgcgctgg ttccgagtgc gacacccgcc ggcctggcgc cggtcgaaca ttttgcacag
      901 gcgcgtccgt cggcgatgtc caacagcatt cgcgacatct cgttccggcg aggaaaggat
      961 ggtgagggcc ggatcactgt cgacctgact gacgccaatg ccggcatcga catcaagcag
     1021 caggggtcga atctggtggt ggacttcgcc aaggtgacgc ttcccgaggc cttgcggaag
     1081 cggctcgatg tgaccgattt tgcgacgccc gtgctgaccg tgacgaccgt cgagaagggg
     1141 cccaacaccc gcatgacgat taccccgcgt ggcttgtggg agcacaatgc ttaccagagc
     1201 gacaaccagt tcgtcattga agtgaagccg atcatcgaga atcccaacaa actggtgcag
     1261 gggagccgcg gcggctatca gggcgagaag ctctccctga atttccagaa tgtcgacgtg
     1321 cgccgcctgt tgcaggtcat cggcgagttc acgggcatga acatggtcgt cagcgattcc
     1381 gtgggcgggt cgatcacgct gattctcaag gacgtgccat gggaccaggc gctggacatc
     1441 atcatgcagc agaagggtct cgacatgcgc aagaatggga atgtcattct tattgcgccg
     1501 cgggaggaaa tcgccaccaa ggagaaactc gaattcgaat cgaaagcgca aatcggcgac
     1561 ctggaaccct tgcagaccga gagtttccag atgaacta
//
LOCUS       DAOSIT010000580         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_66985_length_1598_cov_7.716785, whole genome shotgun sequence.
ACCESSION   DAOSIT010000580 DAOSIT010000000
VERSION     DAOSIT010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66985_length_1598_cov_7.716785"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1598
                     /locus_tag="PLL14_11200"
     CDS             160..>1598
                     /locus_tag="PLL14_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOG04540.1"
                     /translation="MVKVLQQILRWLFMRIENLFNVAFGDKMNPFYHLGTISFWQFWL
                     LVGSGIYLYIFADTGVHDAFESVERITHAQWWAGGILRSIHRYATDGMILTMLLHMLR
                     HFAYDRYRGFRSFSWLTGVALLWLIYIAGVNGFMLVWDKLAQFVVIAAAEWFDVLPMF
                     NGTLIRNFLYLESVNSRLFTLLAFIHLGAPLIVAFIMWVHVQRVPRAHINPPRPIAIA
                     VTLTFLALALVKPILSQGGEADMSVVPSNIDFDWFELPVLALVYVVNPLHLWYWVLGL
                     TAVLFLTPWLPPKRRGSALARTSITFHPDHKAVSARFGETLLDAGLRQDIDLPYECRN
                     GGCGVCKCTVLQGRVDPGLYQPSALSAAELAQGKVLLCCATALEDVEIEYQASTAPRK
                     FQEYQARVVNMQKLTHDVMQVHLKLPEGERIDFKAGQYINIILEDGQRRAFSFANPPQ
                     QAEFVELQIRLMPGGRFTTHVFERMQEGD"
BASE COUNT          292 a          537 c          448 g          321 t
ORIGIN      
        1 gcgccgggcc aggtcatggt gatcgacttc aaccccgacc gcggtggcct gttcttcaaa
       61 tgatcgccac tcctgccagc aaagctgcac gctgccggca actccgcgct attccagcag
      121 ccccgcacgc ctcacccttc atctgaaccg acatcgacca tggtcaaagt cctgcaacaa
      181 atcctgcgct ggctgttcat gcgcatcgag aatcttttca acgtcgcctt cggcgacaag
      241 atgaaccctt tctatcacct ggggacgatc agcttctggc aattctggtt gctggtgggc
      301 tccggcattt acctctacat cttcgccgat accggggttc atgacgcttt cgaatcggtc
      361 gagcggatca cgcacgcgca gtggtgggcg ggcggcatcc tgcgcagcat tcaccgctac
      421 gccaccgacg gcatgatcct gaccatgctg ctgcacatgc tgcggcactt cgcctacgac
      481 cgctaccgtg gctttcgctc gttctcctgg ctgaccggcg tcgccctgct gtggctgatc
      541 tacatcgccg gcgtcaacgg cttcatgctc gtctgggaca aactggcaca gttcgtggtc
      601 atcgccgccg ccgagtggtt cgatgtcctg ccgatgttca acggcacgct gatccgcaac
      661 ttcctgtacc tcgaaagcgt caacagccgc ctctttacgc tgctcgcctt catccacctc
      721 ggtgcgccgc tgatcgtcgc cttcatcatg tgggtgcatg tgcagcgcgt accgcgcgcg
      781 cacatcaacc cgccgcgtcc gatcgccatc gcggtcaccc tgacgtttct cgcgctcgcc
      841 ctggtcaagc cgatcctcag ccagggtggc gaggccgaca tgtcggtcgt gccgagcaac
      901 atcgacttcg actggttcga gttgccggtc ctggcgctgg tctatgtcgt caacccattg
      961 cacctctggt actgggtact gggcctcacc gccgtcctct tcctgactcc ctggctgcca
     1021 ccgaaacggc gggggtctgc gctggcacga acctcgatta ccttccaccc ggatcacaag
     1081 gcggtcagcg cacgttttgg cgaaacgctg ctcgacgccg gtctgcggca ggacatcgat
     1141 cttccctacg aatgccgcaa cggcggctgt ggggtatgca agtgcaccgt cctgcagggc
     1201 agggtcgacc ccggcctcta tcagccaagc gcgctgtcgg ccgccgaact ggcgcagggc
     1261 aaagttctgc tgtgctgcgc cacggcactc gaagatgtcg aaatcgagta ccaggcgagc
     1321 acggcgccaa ggaaattcca ggagtaccag gcccgcgtgg tgaacatgca gaagctcacg
     1381 cacgacgtca tgcaggtgca cctcaagctg ccggaaggag aacggatcga cttcaaggcc
     1441 gggcaataca tcaacatcat tctcgaagac ggccagcggc gagcgttctc gttcgccaac
     1501 ccgccacaac aggcggaatt cgtcgaactg cagatccgtc tgatgcccgg tggccgtttc
     1561 acgacacacg tttttgaaag gatgcaggaa ggcgacca
//
LOCUS       DAOSIT010000581         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_67476_length_1590_cov_8.272313, whole genome shotgun sequence.
ACCESSION   DAOSIT010000581 DAOSIT010000000
VERSION     DAOSIT010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67476_length_1590_cov_8.272313"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1590
                     /gene="nuoG"
                     /locus_tag="PLL14_11205"
     CDS             <1..>1590
                     /gene="nuoG"
                     /locus_tag="PLL14_11205"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOG04541.1"
                     /translation="PHFCYHKKLSIAANCRMCLVQVEKAPKPLPACATPVTNGMKVFT
                     HSELAIKAQKGVMEFLLINHPLDCPICDQGGECQLQDLAVGYGGSASRFQEDKRVVFH
                     KDVGSLISMQEMARCIHCTRCVRFGQEIAGVMELGMASRNMHSEIMTFVGRSVDSELS
                     GNMIDLCPVGALTSKPFRYSARSWELSRRRSISPHDSIGANLTVQIKNNVVMRVLPRD
                     NEEVNECWISDKDRFSYEALNSAARLGKPMVRVDGTLREVEWSVALDYVSHALKDVVG
                     SSGADSLASLASPHATLEELYLLQKLTRALGSGSIDFRPRRRDFSTDGKLAGVPWLGL
                     RLSEIKDLDGALVVGSFLRKDHPLFAQRLRQLGKKWGKVSLLSVGGDDPLIRLHAQLT
                     VSPADLPLALAAVVKSAAQLRSEAVPAGLDSVEPCASSQAIAKSLVEGERGAIFLGNV
                     VEQHPHAAQLHTLALELARLTGASCGFIGEAANSVGGYVAKALPTALNAYEMFAQPRK
                     AYVLLGVEPDLDCHNPHQAVA"
BASE COUNT          296 a          478 c          495 g          321 t
ORIGIN      
        1 tcccgcattt ctgctatcac aagaagcttt cgatcgccgc caactgccgc atgtgtctgg
       61 tgcaggtaga gaaggcgccg aaaccgttgc ccgcctgtgc cacaccagtg accaacggca
      121 tgaaagtgtt tacgcactcc gaactggcca tcaaggcgca gaagggtgta atggagtttc
      181 tgctgatcaa tcacccactc gattgtccga tctgtgatca ggggggcgag tgtcaactgc
      241 aggatctggc cgtgggttat ggtggcagtg cctcccgctt tcaggaagac aagcgggttg
      301 tctttcacaa ggatgtgggt tcgctgatct cgatgcagga gatggcgcgg tgcatccact
      361 gcacgcgatg cgtccgcttc ggacaggaga tcgccggagt catggaactg ggcatggcca
      421 gccgcaatat gcactccgag atcatgacct ttgttggtcg ctcagtcgac tccgagcttt
      481 ccggcaacat gatcgatctt tgcccggttg gcgcgctgac ttccaaaccc ttccgctatt
      541 ctgcccgtag ttgggagctg tcgcggcgtc gctcgatcag tccgcacgat agtatcggcg
      601 ccaacctgac cgtacagatc aagaacaacg tggtcatgcg cgtgttgccg cgcgacaacg
      661 aagaggtcaa cgagtgctgg atctcggaca aggatcgttt ttcctacgag gcgctgaact
      721 cagcggcacg cctgggcaag ccaatggtcc gcgtggatgg cacattgcgc gaggttgagt
      781 ggagcgttgc tctcgattac gtctcgcatg cgctcaagga tgtcgtcggc agcagcggtg
      841 ccgactcgct ggcgtccctg gcctcgccgc atgcgaccct cgaggaattg tacttgctcc
      901 agaaactgac tcgcgccctc gggtcgggga gcattgattt tcgtccccgg cgacgcgact
      961 tttcaactga cggcaaactt gccggtgtgc cgtggttggg cctcaggctc tccgaaatca
     1021 aggacctcga cggcgctctg gtagtcggca gtttcctgcg caaggaccat ccactgttcg
     1081 cgcaacgtct gcgtcagctc ggcaagaagt ggggcaaggt cagcctgctg tctgtcggtg
     1141 gcgacgatcc gctgatcagg ttgcacgcac agctgacggt gtcaccggcc gacctgccgc
     1201 tggcccttgc cgcggtagtc aagtctgcgg cgcaactcag gagcgaggcc gtgccggcgg
     1261 gtctcgattc ggtggaaccc tgcgcaagca gccaggccat cgccaagagc ctggtcgaag
     1321 gagaaagggg tgccattttc ctgggtaacg tcgtcgagca gcatccgcac gcagcccaac
     1381 tgcacaccct ggcgcttgaa ctcgcgcgcc tcaccggggc cagttgcgga tttatcgggg
     1441 aagcggccaa cagtgtcgga ggctacgttg ccaaggcgct gccgacggcg ctcaatgcct
     1501 acgaaatgtt cgcgcagccc cgcaaggctt acgttctgct cggcgtcgag ccggacctcg
     1561 actgccacaa cccgcaccag gcggtggctg
//
LOCUS       DAOSIT010000582         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_67537_length_1589_cov_10.808996, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000582 DAOSIT010000000
VERSION     DAOSIT010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67537_length_1589_cov_10.808996"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="mutL"
                     /locus_tag="PLL14_11210"
     CDS             complement(<1..938)
                     /gene="mutL"
                     /locus_tag="PLL14_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG04542.1"
                     /translation="MPESVRIHLLPDLLISQIAAGEVVDRPASVLKELLENALDAGSS
                     TIQIQLEEGGVKLIRVSDDGIGIARDELALALLRHATSKINSLDDLERVGTLGFRGEA
                     LASVAAVARVTLTSRQRGEQASKQHAWRLAAATGATPEPAALLAGTVVEMRDLYYNTP
                     ARRKFLKSDNTEFAHCTEAVKRIALAHPEVAFTLSHNTRVNLHLPRSDARSRAGSLLG
                     EDFLAEARCLDVQAGPLRVAGYCALPAYSRARSDAQYCYVNGRFVRDKLLGHAVREAY
                     QDLLHGSRYPAYCLFLEIDPAGIDVNVHPQKTEVRFR"
     gene            1008..>1589
                     /locus_tag="PLL14_11215"
     CDS             1008..>1589
                     /locus_tag="PLL14_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOG04543.1"
                     /translation="MEYLTSFFDIILHLDKHLAMLVQQYGPWIYAILFAIIFSETGFV
                     VTPFLPGDSLLFVAGALAALGGMEIGLLLAVLSAAAVLGNMLNYQIGRFLGPKVFHWE
                     QSRFFNRSALEKTHAFYEKHGGKTLVISRFLPLFRTFAPFVAGIGAMSYAKFTAFNLI
                     GGLAWVGSLTLAGYWFGNLPWIQQNLTLVIVAII"
BASE COUNT          265 a          487 c          513 g          324 t
ORIGIN      
        1 cggaaccgga cctcggtctt ctgcgggtgg acgttgacgt cgatgccggc gggatcgatc
       61 tccaggaaca ggcagtaagc cgggtaacgg ctgccatgca gcaggtcctg gtaggcctcg
      121 cgcacggcat gcccgagcag cttgtcgcgg acgaaacggc cgttcacata gcagtactgg
      181 gcatcgctgc gcgcgcgtga ataggccggc aacgcgcagt agccggccac gcgcagtgga
      241 ccggcctgga cgtcgagaca gcgcgcttcg gccagaaagt cctcgccgag gaggctcccg
      301 gcacgcgagc gtgcatcgct cctgggcagg tgcaggttga cccgagtatt gtgcgagagg
      361 gtgaaggcga cttcgggatg ggccagcgcg atacgcttga cggcctcggt gcaatgcgcg
      421 aattcggtat tgtcggactt gaggaatttc cggcgggcag gcgtgttgta atagaggtca
      481 cgcatttcga ccaccgttcc ggcgagcaag gcggccggtt cgggcgttgc cccggttgcg
      541 gccgccagac gccaggcgtg ttgcttgctt gcctgctcgc ccctctggcg gctggtcagc
      601 gtgacgcggg cgacggcggc caccgaagcc agcgcctcgc cacgaaagcc gagggtaccc
      661 acccgttcga ggtcgtccag ggaattgatt ttcgacgtcg cgtgccggag cagcgccagc
      721 gccagttcgt cgcgggcgat gccgataccg tcgtcactga cgcggatcag cttgacgccg
      781 ccttcttcga gctggatctg gatcgtcgaa ctgccggcgt caagggcgtt ttcaagcagt
      841 tccttgagta ccgacgccgg ccgatcgacg acttcgccgg cagcgatctg gctgatcagc
      901 aggtcgggca gcaggtggat tctcacggat tcgggcatcg cccgattata ccggcccggc
      961 ttccggtaga atcgcgcctt gccgatggcc accccttttt cgcgacgatg gaatacctga
     1021 ccagcttttt cgacatcatc ctgcacctcg acaaacacct ggcgatgctg gtgcagcaat
     1081 acgggccgtg gatctacgcc attctcttcg ccatcatctt cagcgaaacg ggtttcgtgg
     1141 tgactccctt tctgcctgga gactcgctgc tgttcgtcgc cggcgcgctg gctgcactgg
     1201 gaggcatgga gatcggcctg ttgctcgcgg tgctgagtgc cgccgcggta ctcggcaaca
     1261 tgctcaacta ccagattggt cgctttctcg ggccgaaagt cttccactgg gaacaatcgc
     1321 gctttttcaa ccgatcggca ctggaaaaga cgcacgcctt ctacgaaaag cacggcggca
     1381 agaccctggt gatttcacgc ttcctgccgc tgtttcggac ttttgcgccc tttgtcgccg
     1441 gcattggcgc gatgagttat gccaagttca cggctttcaa tctgatcggt ggcctggcct
     1501 gggtcggttc gctgacgctg gccggctact ggttcggcaa cctgccctgg attcagcaga
     1561 atctgacgct ggtgatcgtg gccatcatc
//
LOCUS       DAOSIT010000583         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_67542_length_1589_cov_7.885267, whole genome shotgun sequence.
ACCESSION   DAOSIT010000583 DAOSIT010000000
VERSION     DAOSIT010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67542_length_1589_cov_7.885267"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLL14_11220"
     CDS             <1..557
                     /locus_tag="PLL14_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOG04544.1"
                     /translation="KAPIYVDDTGGLDVMDMRARARRMKKRYKVELIVIDYLQLCNCR
                     EFARQGRQIETSQISGQIKAMAKELNLPVIVLSQLSRAPEQRGDKTNKPKLSDLRDSG
                     AIEQDADVVLLLRRPCKAPGDPEFEDERLAIVDVAKHRNGPTGEVRLDFEDALTRFGD
                     RADPGLSATADIEPDSSAYLPDDQ"
     gene            632..>1589
                     /locus_tag="PLL14_11225"
     CDS             632..>1589
                     /locus_tag="PLL14_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG04545.1"
                     /translation="MALAFLLAILIGAFLLALPIANTNGQWRDAISALFTATSATCVT
                     GLSVIDVGTELTRFGQIVLITLIQLGGLGITTFGTFLLVLIGRRLSVQNEFVLMDAYG
                     VEAVKGIRALLLWTIGFTLLFEGIGTLLLWARYMAPHPSLPLPYDPWTALYYAIFHAI
                     SAFCNAGFSLHRDSLIAFQHDPVYLFTISILVVAGGLGFLVHYNLVTIKFWRRNRMTR
                     GRITLHTKVVLIATSVLILLGLVFTFLLEMPHTLKGLPLADKLICALVHGISARTAGF
                     NVVPMGDIQEATRFITALLMLIGGSPGSAAGGIKTTTLVVLIM"
BASE COUNT          293 a          548 c          410 g          338 t
ORIGIN      
        1 gcaaagcacc gatttatgtg gacgacaccg gcggccttga cgtcatggac atgcgtgccc
       61 gcgcccgccg catgaagaag cgctacaagg tcgagttgat cgtgatcgac tacctccagc
      121 tctgtaactg ccgcgaattc gccagacagg ggcgccagat tgaaacctcg cagatctccg
      181 gccagatcaa ggccatggcc aaagagctga acctgccggt catcgtgctc agccaactca
      241 gccgtgcccc ggaacagcgc ggcgataaaa ccaacaagcc caaactctcc gacctgcgcg
      301 actccggtgc catcgaacag gatgccgatg tcgtgttgct gctgcgccgc ccctgcaagg
      361 cacccggcga cccggagttc gaagatgagc gtctggccat tgtcgatgtc gccaagcacc
      421 gcaacggccc caccggtgag gtccgactcg atttcgagga cgccctcaca cgcttcggcg
      481 accgcgccga ccccggcctc agcgcaaccg ccgatatcga gccagattcc tcggcctatc
      541 ttcccgacga tcagtgacaa cctgccctgc tgccatgcga aaaacggcca agctcgagaa
      601 aaacccgcac cctctgggac cgcaggtgcc catggccctc gcctttttgt tggcgatcct
      661 gatcggtgcc ttcctgctcg cgttgcctat tgccaacact aacggtcaat ggcgcgacgc
      721 catctctgcg ctcttcaccg ccaccagtgc cacctgcgtc accggacttt cggtgatcga
      781 cgtcggcact gaactcacgc gctttggcca gatcgtgctc attacattga ttcaactcgg
      841 tggccttggt attacgacct tcggcacctt cctgctggtg ctcatcggcc gccgcctcag
      901 tgtgcagaac gagtttgtgc tgatggacgc ctacggcgtt gaagcggtga aaggcatccg
      961 tgccctgctg ctctggacca taggtttcac cctgcttttc gagggcatcg gcaccctgtt
     1021 gctctgggca cgctacatgg cacctcaccc ctcccttccc ctcccctacg acccgtggac
     1081 tgccttgtat tacgccatct tccacgcaat cagcgccttt tgcaacgccg gtttttcgct
     1141 acaccgcgac agcctaatcg ccttccagca cgatcccgtt tatttgttca ccatcagtat
     1201 tctggttgtc gcgggtgggc tcggcttcct cgtccactac aatctggtga ccatcaagtt
     1261 ttggcggcgt aaccgcatga cgcgcgggcg catcaccctg cacaccaaag tcgttctgat
     1321 cgccacctca gtgctgatcc tgctcggcct cgtgtttaca tttctgttgg aaatgcccca
     1381 cacgctgaag ggccttcccc tcgccgacaa actgatctgc gcacttgttc acggcatttc
     1441 cgcacggact gccggtttca acgtcgtgcc gatgggtgac atccaagagg ccacccgctt
     1501 catcactgcc ctgctcatgt tgatcggcgg ctcgcccggt tccgctgcgg gtggcatcaa
     1561 gaccaccacg ctggtggtac tgatcatga
//
LOCUS       DAOSIT010000584         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_67601_length_1588_cov_6.181344, whole genome shotgun sequence.
ACCESSION   DAOSIT010000584 DAOSIT010000000
VERSION     DAOSIT010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67601_length_1588_cov_6.181344"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLL14_11230"
     CDS             complement(<1..884)
                     /locus_tag="PLL14_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG04546.1"
                     /translation="MIRPTDSLQLALRAITAHRLRSFLTLLGIAIGIAAVILLTSIGE
                     GIHRFVLAEFTQFGTNVLSIAPGKTRTSGHLPTGIPTSVRPLTLDDARALAQLPHITG
                     MTATVWGNTEVNANGRLRRTTVNGVSAEMLKVFSIKVRSGQFLPEEDSDSARAFIVLG
                     AKLKDELFGTSNPLGARIRVGGLQFRVIGVLEAKGQFLGVDLDDAAYIPTARALELYN
                     RDGIMKIDLSYEEGVPAARVGALVTAMLKARHGREDFTITTQEDMLRTLSNILDVLTM
                     AVGALGSISLLVGGVGIVT"
     gene            complement(900..>1588)
                     /locus_tag="PLL14_11235"
     CDS             complement(900..>1588)
                     /locus_tag="PLL14_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG04547.1"
                     /translation="GQLVRIGDRRFRIVGVLAASGQGLGMNTDELVIVPVALAQAMFN
                     SNTLFRILVEATNRDTIELAKTQAGEIIKLRHEGEQDVTIITQDAVLATFDRLLGTMT
                     VAVAGIAAISLAVAGILVMNVMLVSVTQRTAEIGLLKALGATGATIRNAFLTEAAMLS
                     VAGALLGFVLGQSGAALIRQLYPAYPAFPPDWAVLAGIGTALVTGILFGVLPARRAAR
                     LDPVQALARR"
BASE COUNT          276 a          490 c          548 g          274 t
ORIGIN      
        1 gtgacaatgc cgacgccacc gacgagcaat gaaatgctgc cgagcgcgcc gaccgccatg
       61 gtcagcacat cgagaatgtt cgacagcgtc cgcaacatgt cttcctgtgt ggtgatggtg
      121 aaatcttccc gcccgtgccg ggctttcagc attgccgtga ccagcgcgcc gaccctggcg
      181 gccggtacgc cttcctcata cgagaggtca atcttcatga tcccgtcgcg attgtagagt
      241 tcgagcgcgc gcgcggtcgg gatgtaagcc gcgtcgtcga gatcgacgcc gagaaactgt
      301 cctttggctt ccagcacgcc gatgacgcga aactgcagac cgccgacgcg tatccgcgca
      361 cccagcggat tgctggtgcc gaacagttca tccttgagtt tcgcgccgag gacgatgaag
      421 gcgcgcgcac tgtcggagtc ttcctcgggc aggaactggc cgctgcggac cttgatgctg
      481 aaaaccttga gcatctcggc gctgacccca ttgacggtgg tgcgtcgcag gcggccgttg
      541 gcattgacct cggtgttgcc ccagacggtg gcggtcatgc cggtgatgtg cggcagctgg
      601 gcgagcgcgc gcgcgtcatc aagggtcagc gggcgcaccg aggtcgggat gccggtcggt
      661 aggtgcccgg aggtcctggt cttgcccggg gcgatgctga gcacgttggt gccgaactgg
      721 gtgaattcgg cgagcacgaa tcggtggatg ccttcaccga tcgaggtcag caggatcacc
      781 gcggcgatgc cgatggcaat accgagcaac gtcaggaagc tgcgcaaccg gtgcgcggtg
      841 atcgcgcgca gcgcgagctg cagggagtcg gtcgggcgga tcatgggcgg cgtgcaggct
      901 cagcgccggg ccagcgcctg caccgggtcg agtcgtgctg cccggcgtgc cggcagcacc
      961 ccgaacagga tgccggtgac tagcgccgtg ccaatccccg cgagcaccgc ccagtcgggt
     1021 ggaaacgccg gataggcggg gtagagttgc cggatcagcg cggcgccaga ctggccgagc
     1081 acaaacccga gcagagcgcc ggccacggac agcatcgccg cttcagtcag gaaggcgttg
     1141 cggattgtcg cgcccgtggc gccaagtgcc ttgagcaggc cgatttcggc ggtccgctgg
     1201 gtcaccgaga cgagcatcac gttcatcacc agaatgccgg cgacggccag actgatcgct
     1261 gcgatgcccg caacggcgac ggtcatggtg ccgaggagtc ggtcgaaggt cgcaaggacc
     1321 gcgtcctggg tgatgatggt cacgtcctgc tcaccttcat gtcgaagctt gatgatctcg
     1381 ccggcctggg tcttggccag ttcgatggtg tcgcggttcg tggcttcgac aaggatgcgg
     1441 aacagcgtgt tgctgttgaa catcgcttgc gccagtgcca ccggcacgat gaccagttcg
     1501 tcggtattca tgcccaggcc ctggccgctc gcggcgagga cgccgacaat ccggaagcgt
     1561 cggtcaccga tacgcaccag ttgcccca
//
LOCUS       DAOSIT010000585         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_68025_length_1581_cov_10.066841, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000585 DAOSIT010000000
VERSION     DAOSIT010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68025_length_1581_cov_10.066841"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PLL14_11240"
     CDS             complement(<1..1406)
                     /locus_tag="PLL14_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG04548.1"
                     /translation="MSETKSVYAAYSGNTYLFGGNAPYVEEMYENYLANPGSVPDTWR
                     SYFDALQHVPASDGSDAKDVPHLPVINAFAERAKQGGTKVVQASADVEMGRKRTAVQQ
                     LIAAYRNVGQRWADLDPLQRTERPVIHELEPSFYGFSDADQEAVFDTSNTFFGKDRMS
                     LRELLNALRETYCGTVGPEYMYITNQAEKRWWQQKLESIRSKPSFNTDKKKHILDRLT
                     AAEGLERFLHTKFVGQKRFSLEGGESFIAAMDELIQQAGAKGVQEIVIGMAHRGRLNV
                     LVNTLGKMPGDLFAEFDHTAPEDLPAGDVKYHQGFSSDVTTPGGPVHLSLAFNPSHLE
                     IVNPVVEGSVRARMDRRGDRRGDQVLPVLVHGDAAFAGQGVNQETLALAQTRGYTTGG
                     TVHLIINNQIGFTTSDPRDLRSTLYCTDIVKGVESPVLHVNGDDPEAVVLATQLALEY
                     RMEFRKDVVVDIICFRKLG"
BASE COUNT          276 a          481 c          506 g          318 t
ORIGIN      
        1 cccagcttgc ggaagcagat gatgtccacc accacgtcct tgcggaattc catccggtac
       61 tcaagcgcca gttgggtcgc cagcacgacg gcctccggat cgtcgccatt gacatgcagc
      121 accggcgatt cgaccccctt gacgatgtcc gtgcagtaca gggtggaacg aaggtcgcgc
      181 ggatcggagg tggtgaagcc gatctggttg ttgatgatca ggtgcaccgt gccaccggtg
      241 gtgtagccgc gggtctgggc cagtgccagg gtttcctggt tgacgccctg cccggcaaag
      301 gcggcatcgc cgtgcaccag caccggcagc acctggtcgc cacggcggtc gccgcggcgg
      361 tccatgcgcg cacgcaccga gccctccacc acggggttga cgatttcaag gtgcgagggg
      421 ttgaacgcca gcgacaggtg aaccggaccg cccggggtgg tcacgtcgga actgaacccc
      481 tggtgatact tcacgtcgcc ggccggcagg tcttcgggtg cggtgtggtc gaactcggcg
      541 aacaggtcgc caggcatctt gcccagcgtg ttcacgagca cgttcaggcg accccgatgg
      601 gccatgccga tcacgatttc ctgcacgcct ttggcgccgg cctgctggat cagctcatcc
      661 atcgcggcaa tgaagctctc gccgccttcg agcgagaaac gcttctggcc gacaaacttc
      721 gtgtgcagga agcgctccag accttcggcg gcggtcaggc gatcgaggat gtgcttcttc
      781 ttgtcggtgt tgaagctggg cttgctgcga atgctctcca gcttctgctg ccaccagcgc
      841 ttttcggcct ggttggtgat gtacatgtat tccgggccga cggttccgca ataggtctcg
      901 cgcagggcat tgagcagttc gcgcagggac atgcggtcct tgccgaagaa ggtgttgctg
      961 gtgtcgaaaa cggcttcctg gtccgcgtcg ctgaacccgt agaacgacgg ctcgagttca
     1021 tgaatgacgg ggcgctcggt gcgttgcagg ggatccagat ctgcccagcg ctgtccgaca
     1081 ttgcggtacg cggcaatcag ctgctgcacg gccgtgcgct tgcgacccat ttcgacgtcg
     1141 gcgctggctt gcaccacctt ggtgccgccc tgcttggcgc gctcggcgaa cgcattgatc
     1201 acgggcaggt gcggaacatc cttggcgtca ctgccgtcgc tggccggaac atgctggagc
     1261 gcatcaaaat agctgcgcca cgtgtcgggc acgctgccgg gattggcgag gtagttttcg
     1321 tacatctctt cgacgtaagg cgcattgccc ccgaagagat aggtgttgcc tgaataggcg
     1381 gcgtagacgg actttgtctc actcatgttt cgctgacctc aattcccctg cagggaacat
     1441 tagctggttg aaatacctcc cgcgacacgg ctgaaccgat ctgcggatgc gactgtggct
     1501 taggaagggc cctggaaagc cttgcgattt tgccatcgaa accctgcacg caactgacaa
     1561 gctccgcgag agaccccatg a
//
LOCUS       DAOSIT010000586         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_68477_length_1574_cov_8.630020, whole genome shotgun sequence.
ACCESSION   DAOSIT010000586 DAOSIT010000000
VERSION     DAOSIT010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68477_length_1574_cov_8.630020"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1574)
                     /gene="pheT"
                     /locus_tag="PLL14_11245"
     CDS             complement(<1..>1574)
                     /gene="pheT"
                     /locus_tag="PLL14_11245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOG04549.1"
                     /translation="MAGLEVEAEETVAPHFDQVVVAQVLSVDKHPDADRLNVCRVDVG
                     SGEPLQIVCGAPNAAAGIKVPCALPGAELPGGFRIKVAKVRGVESSGMLCSARELGIA
                     EDASGLLLLAADAPVGEDIRRHLDLDDRLLTLKLTPNRADCLSLEGVAREVAALTGTP
                     ARFTEVTEVEVTLAVARAVVLDAPAACPRYCGRIIAGVDGTAATPAWMRQRLERSGVR
                     PISALVDITNYVMLELGQPLHAFDNSRLQGAIHARMAQAGETISLLNEQTLTLQPDIL
                     LISDAQRPVAMAGIMGDEASGITLATTEVFLEAAFFAPAAIAGRARRYGFVSDASHRF
                     ERGVDFAGTRRALERATRLVIDICGGQAGPVSEASASLPARLPVRLRPARVAKVLGLP
                     LTAERIGELFSRLALPYTREGQDFVVTPPSYRFDIEIEEDLIEEIARLHGYENIPSAA
                     PQAVLTMLPQTEQARPLARVRQILADCGYQEVVNFAFVEEAWETDFAANEQLIRLANP
                     IASHMSVMRSSLIGGL"
BASE COUNT          249 a          526 c          550 g          249 t
ORIGIN      
        1 caacccgccg atcagcgagg atcgcatgac gctcatgtgg ctggcaatcg ggttggccag
       61 gcggatcaat tgctcgttgg cggcgaaatc cgtttcccag gcttcttcga cgaaggcgaa
      121 gttgaccact tcctggtagc cgcagtcggc caggatctgc cgcacgcgcg ccagcggccg
      181 tgcctgctcg gtctgcggca gcatcgtcag gaccgcctgc ggcgctgccg agggaatgtt
      241 ttcgtagccg tgcaggcgag cgatctcctc gatcaggtct tcctcgatct cgatgtcgaa
      301 gcgatagctc ggcggcgtga cgacgaagtc ctggccctcg cgggtatacg gcaaggccag
      361 gcgcgagaac agttcgccga tacgctctgc ggtcaacggc agcccgagca ccttggcgac
      421 gcgcgccggg cgcaggcgca ccggcagccg cgccggcagg ctcgccgaag cctcgctgac
      481 cggccctgcc tgtccgccgc agatatcgat caccagccgt gtggcccgct cgagggcgcg
      541 ccgcgtgccg gcgaaatcga cgccgcgctc gaaacgatgc gaagcgtccg agacgaagcc
      601 atagcgccgc gcacgcccgg caatggccgc cggcgcgaag aaggccgctt cgaggaacac
      661 ctcggtggtg gccagggtga tgccgctggc ttcgtcgccc atgatcccgg ccatcgccac
      721 cggacgttgt gcatcgctga tcagcaggat gtcgggctgc agggtcagcg tctgctcgtt
      781 gagcaggctg atcgtctcgc cggcctgcgc catgcgggcg tggatggcgc cctgcaggcg
      841 gctgttgtcg aaagcgtgca gcggctgccc gagttcgagc atcacgtagt tggtgatgtc
      901 gaccagcgcc gagatcggac gcacgccgct gcgctccagg cgctgccgca tccacgccgg
      961 tgtggcggcg gtgccgtcga cgccggcgat gatccgaccg cagtaacgcg ggcaggctgc
     1021 gggcgcgtcg agcacgacgg cgcgcgcgac cgcgagcgtg acttcgacct ctgtgacttc
     1081 ggtaaaacgg gcgggggtgc cggtcagtgc cgcgacctcg cgggccaccc cttcgagcga
     1141 cagacagtcg gcccggttgg gtgtgagctt gagcgtcagc aggcggtcat cgagatcgag
     1201 atggcgacgg atgtcttcgc ccaccggcgc atcggcggcc agcagcagca gtccggaggc
     1261 gtcctcggca atcccgagtt ccctggccga gcagagcatt cccgaggatt cgacgccgcg
     1321 taccttggcc accttgatcc ggaaaccgcc gggaagttcg gcgcccggca gcgcacatgg
     1381 caccttgatg ccggccgcgg cgttcggcgc accacagacg atctgcagtg gttcacccga
     1441 accaacgtcg acgcggcaga cgttgaggcg gtcggcgtcc ggatgcttgt cgacgctgag
     1501 cacctgcgcg acgacgactt ggtcgaagtg cggcgcgacc gtctcttcgg cttccacttc
     1561 gaggccggcc atgg
//
LOCUS       DAOSIT010000587         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_68589_length_1572_cov_9.340145, whole genome shotgun sequence.
ACCESSION   DAOSIT010000587 DAOSIT010000000
VERSION     DAOSIT010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68589_length_1572_cov_9.340145"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1572
                     /locus_tag="PLL14_11250"
     CDS             <1..>1572
                     /locus_tag="PLL14_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG04550.1"
                     /translation="FAASPQGLFEPLAGQVRELEVAAADGRLLATHSAVIVDAERVVV
                     QCDLVEGAPLLRLRAASRTLVARPLHADVRRNLCLLDVPGLSGQPVGLATALPSPGAR
                     VLAVSNALGLGIGVSDGVVAAIRESGGEALIQHTAPVAPGSEGGGLFDESGRLLGVIR
                     YRRLDGQNVNFAAPAAWIPEIAARAAAQTELREWRGQAAAFERDARWAELATHAAERT
                     RRAADEPEAWFWLAWAKEAQRDWPAAEQAYREAARRAAGDVTVGLGLARALINLDRLD
                     EALTTARQLLAVHAEDTRVWAMIGWIEIRRKQPQAAATALDEAIRLAPWNTTALYLSA
                     TLAADHGDWPKALRLFRQLTRAEPANALWWIKLAEAQYRAQDYQRALNAAEKLLALSP
                     EHADGWLWKGAALSALGRRKEGIDALRAALARGPLSPAWAWIFLGNTYYALRLYPEAI
                     QAYREVAKFEAQAESVQDSLGVALKDGGQFDEALRLFEAARLKRPEDPFVWRQVGYVN
                     GLMGRSEAMIAALEQS"
BASE COUNT          221 a          516 c          604 g          231 t
ORIGIN      
        1 gttcgccgct tcgccgcagg gccttttcga accgcttgcg gggcaggtgc gcgaactcga
       61 agtggcggca gccgacggtc gtctgctggc cacgcactcg gcggtgatcg tcgacgcgga
      121 gcgggtggtc gtccagtgcg acctcgtcga aggggcgccg ctgctgcgcc tgcgcgcggc
      181 gagtcgaacg ctggtcgcgc gcccgctgca cgcggatgtg cgccgcaatc tgtgcctgct
      241 cgacgttccc ggcctgtccg ggcagccggt ggggctggcg accgcgctgc catcccccgg
      301 cgcgcgcgtg ctggcggtga gcaacgccct cgggctcggc atcggcgttt ccgacggggt
      361 cgtcgccgcg atccgcgaga gcggcggcga ggcgctgatc cagcacacgg cgccggttgc
      421 cccagggtcg gaaggtggcg gcctgttcga cgaatccggc cggctgctgg gggtgattcg
      481 ttatcgccgg ctggatgggc agaacgtgaa ttttgccgcg ccggccgcgt ggatcccgga
      541 gatcgccgcg cgtgccgcgg cgcagaccga attgcgggag tggcgtggcc aggcggcggc
      601 tttcgaacgt gacgcgcgct gggctgaact cgccacgcat gccgccgagc gtacccggcg
      661 cgcggccgac gaaccggagg cctggttctg gctggcctgg gcgaaagagg cgcagcgcga
      721 ctggccagcg gccgagcagg cgtatcgcga ggcggcgcgg cgtgctgccg gcgacgtgac
      781 cgtcgggctg gggctggcgc gcgcgctcat caacctcgat cggctcgacg aagcgctgac
      841 cacggcccgc caactgctcg ccgtacatgc cgaggacacg cgcgtctggg cgatgatcgg
      901 ctggatcgag atacgccgca aacaaccgca ggcggcggcc acggcgctcg acgaagcgat
      961 ccggctggcg ccctggaata ccaccgcgct gtacctgtcg gcgacgctgg cggccgacca
     1021 cggcgactgg ccgaaagcct tgcggctgtt ccggcaactg acacgcgccg agccggcgaa
     1081 cgccttgtgg tggatcaagc tggccgaggc ccagtaccgc gcgcaggatt accagcgggc
     1141 gctgaacgcc gccgagaagc tgctggcgct gtcgcccgag catgccgacg gatggctctg
     1201 gaagggcgcc gcgctgtcgg cgctggggcg tcgcaaggag ggcatcgatg cgctgcgggc
     1261 ggcactggcg cgcggcccgc tgagtccggc gtgggcgtgg atcttcctgg gcaacaccta
     1321 ttacgcgttg cgcctgtatc cggaggcgat ccaggcgtat cgcgaggtgg cgaaattcga
     1381 agcgcaggcc gagtcggtcc aggacagcct cggggtcgcg ctcaaggacg gcggccagtt
     1441 tgatgaggcg ctgcgcctgt tcgaggcggc gaggctgaag cgccccgagg atcctttcgt
     1501 ctggcggcag gtcggctacg tcaatggcct catgggacgc agcgaagcga tgattgccgc
     1561 cctggaacaa tc
//
LOCUS       DAOSIT010000588         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_68772_length_1569_cov_8.225231, whole genome shotgun sequence.
ACCESSION   DAOSIT010000588 DAOSIT010000000
VERSION     DAOSIT010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68772_length_1569_cov_8.225231"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLL14_11255"
     CDS             <1..230
                     /locus_tag="PLL14_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOG04551.1"
                     /translation="YVTLAGLFYKRHGSVPKVGDTVKLEGGQLTVLEMDNHRITLLKF
                     EDTAVGDDGVIRLVENSSAADANLASPEAPK"
     gene            complement(338..>1569)
                     /locus_tag="PLL14_11260"
     CDS             complement(338..>1569)
                     /locus_tag="PLL14_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HOG04552.1"
                     /translation="VEEIKEMLDGVIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRC
                     KDAMIFSTQSTHKLLAGLSQASQILVQNSEGRKLDRDVFNEAYLMHTSTSPQYAIIAS
                     CDVAAAMMETPGGTALVEESIAEALNFRSTMRKVEQEWGADWWFKVWGPDDLAGEGLE
                     DRSAWILKPGERWHGFGMLAEGFNMLDPIKATIITPGLDVDGDFAEWGIPAAIVTKYL
                     AEHGIIVEKCGLYSFFIMFTIGITKGRWNTMVTELQQFKDGYDQNLPLWKVMPVFLAK
                     NPRYEGCGLKDLCRQIHGVYAANDVAHLTTRMYLSDMEPAMKPTDAFARMAHREIDRV
                     PIDDLEGRITSTLLTPYPPGIPLLIPGERFNATIVRYLQFARDFNEHFPGFETDVHGL
                     VKAIVDGKPVYSVDCVR"
BASE COUNT          302 a          456 c          480 g          331 t
ORIGIN      
        1 attacgtcac gctcgcgggc ctgttctaca agcggcacgg cagtgtgccg aaggttggcg
       61 acaccgtcaa acttgagggt ggccaactga cggtgctgga gatggacaac caccggatca
      121 cgttgttgaa gtttgaagac accgcggtgg gtgacgacgg cgtaattcgc ctggtcgaaa
      181 actcttcggc ggcggacgcc aacctggcat ctcccgaggc gcccaagtag tcgcctggcg
      241 cgagcgttga gatgtggggt gcatgaatga tgaggtgagg caggcgtcgt ctgcctgcgg
      301 gccgaccgag gcgacgcttg cgcacctctt caactcatca gcgtacgcaa tccaccgagt
      361 agacaggttt gccgtcgacg atagccttca ccaagccatg cacgtcggtt tcgaagcctg
      421 ggaagtgctc gttgaaatcg cgcgcgaact ggagataacg aacgatcgtc gcgttgaaac
      481 gctcacccgg aatcaggagc ggtattcctg gcgggtaggg tgtcagtagc gtactggtaa
      541 tccgcccttc gagatcgtcg atcggaactc ggtcgatctc gcggtgcgcc atcctggcga
      601 aagcgtcggt cggtttcatc gccggttcca tgtccgagag atacatgcgc gtggtcaggt
      661 gcgcgacatc attggccgcg tagacgccgt ggatctgccg gcagaggtcc ttcaggccgc
      721 agccctcgta gcgcggattc ttcgccagga acaccggcat taccttccac agcggcaggt
      781 tctggtcgta accatccttg aactgttgca actcggtgac cattgtgttc catcggccct
      841 tggtaatgcc gatggtgaac atgatgaaga acgaatacag gccgcacttc tcgacgatga
      901 tgccgtgctc ggcgaggtac ttggtgacga ttgctgcggg aatgccccac tcggcgaaat
      961 cgccgtcaac atcaagaccc ggcgtaatga tcgtggcctt gatcgggtcg agcatgttga
     1021 agccttcggc gagcatgcca aagccatgcc agcgttcccc gggcttgagt atccaggccg
     1081 aacggtcttc gagtccttct cccgcgaggt cgtctggtcc ccacaccttg aaccaccagt
     1141 cggcccccca ctcctgctcg accttgcgca tcgtgctgcg gaagtttagc gcttcggcaa
     1201 tcgactcttc gaccagcgcc gtcccgccgg gggtttccat cattgctgcc gccacatcgc
     1261 aggatgcgat gatcgcgtat tgcggcgagg tcgaggtgtg catcaggtaa gcctcgttga
     1321 agacgtctcg gtcgagtttc cgcccttcgg aattttgtac cagaatctgc gaggcctgcg
     1381 agagacccgc caacagttta tgggtcgact gtgtcgagaa gatcatcgcg tccttgcagc
     1441 gcgggcgatc tgcgccgatg gcatgatagt cgccgtagaa atcgtggaag gccgcgtgtg
     1501 gcagccaggc ctcatcgaaa tgcagcgtgt cgatcacgcc atcgagcatt tccttgattt
     1561 cctcgacgt
//
LOCUS       DAOSIT010000589         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_69016_length_1565_cov_9.776159, whole genome shotgun sequence.
ACCESSION   DAOSIT010000589 DAOSIT010000000
VERSION     DAOSIT010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69016_length_1565_cov_9.776159"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLL14_11265"
     CDS             complement(<1..776)
                     /locus_tag="PLL14_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG04553.1"
                     /translation="MKMEQVSANCFAVLNENNLVCDANSGLINLGGGVVIDTQSDLPH
                     GRQMITLFGKVWRDMPRRVINTHEDGDHVWGNQLFAGAEIIAHRRVKELMPHIANPKE
                     TQQLIKGSDRFLTRLLLKALHPGALAVAQQLKQDYNFDGIRLVLPTTVFDERHVLDLD
                     GTEVHLIYVGPCHQIGDTIIHVPKEKVVFAGDVIFRECTPMGWNGTYEKWLKVLDLII
                     SLEPEVIVPGHGPVCGIEGAMEMKAYLEYVREESKRCFGQG"
     gene            complement(1023..1334)
                     /locus_tag="PLL14_11270"
                     /pseudo
     CDS             complement(1023..1334)
                     /locus_tag="PLL14_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
     gene            complement(1344..>1565)
                     /locus_tag="PLL14_11275"
     CDS             complement(1344..>1565)
                     /locus_tag="PLL14_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04554.1"
                     /translation="TARPGSVWTFAPQHLPGNALVEFLHSSIPAFLLRRRLSVAIVLD
                     RVGALHLPKQHEGLQVIRPQRRAEVIDAL"
BASE COUNT          288 a          500 c          471 g          306 t
ORIGIN      
        1 ccctgcccga agcagcgctt cgattcttcg cgcacgtatt cgaggtaggc cttcatctcc
       61 atcgcgccct cgatcccgca caccggtccg tgtccgggga cgatcacctc cggctcgagc
      121 gagatgatga gatcgagaac cttgagccat ttttcgtaag tgccgttcca gcccatgggg
      181 gtgcactcac ggaaaatcac gtcgccggcg aacacgacct tttccttggg cacatggatg
      241 atcgtgtcac cgatctggtg gcacggcccc acgtagatga ggtgaacttc cgtgccgtcg
      301 agatccagca cgtgccgctc gtcgaacacg gtggtcggca gcaccagccg gatgccgtcg
      361 aagttgtagt cctgcttcag ttgctgggca accgccaacg cccccgggtg caaagccttg
      421 agcaacaagc gtgtgaggaa gcgatcggac cccttgatca gttgctgggt ctcctttggg
      481 ttggcaatgt gcggcatcag ctccttgacc cgccggtgcg cgatgatttc ggcacccgcg
      541 aagagctggt tcccccacac gtggtcgccg tcctcgtgcg tattgatcac gcgcctgggc
      601 atgtcgcgcc agaccttgcc gaacagcgtg atcatctggc gtccgtgcgg caggtccgac
      661 tgtgtatcga tcaccacgcc gccgcccagg ttgatgaggc ccgagttggc atcgcacacg
      721 aggttattct cgtttagcac agcgaaacag ttggcgctga cttgttccat tttcatgttg
      781 tcgtcctctt gtcgtcactg cgatcgagta taaacaatcg atacccgaaa gcgcgccccc
      841 gaagggccgg ccgcgtacgt ccatggcccc cagtcagagt gtgcggtcaa tggactcccg
      901 gttcgcccga ggttgatgct tgccgccgta cccgaggcgg aggccagatt catcatagct
      961 gccggctggg aaagaatcac cttggctata cgtagagggc tatagacgcg gacaagacca
     1021 acatcgacga tgccgcgaaa tgcgccttcg ccactgtcgg ccgtggcgat gcgctcgacc
     1081 aggcggcggt acaccgtttc caggcgatgc agcggcgtct cggtttccga gatctgcact
     1141 tcgctggtcg ccaggccgcg cgcccgcgcc cgctcctgca gccagcggcc gaagaaggtc
     1201 ttgcccgatc cgtattcgcc gcggatcgcc ttgaaggtgc cgcggccgga cgccacagca
     1261 gccaactctt cgtcgagagt tccctgcagc cggccataac ccacggccag cgcatccagg
     1321 ccggccttcg gcaccgtacc gcgtcacaag gcgtcgatga cttcggctct gcgctgcgga
     1381 cggatcactt gtaaaccctc gtgctgcttt gggagatgca gggcgccaac acggtcgagg
     1441 acgatcgcca cgctcaatct ccgcctgagc aggaatgctg gaatgctgga atgcaggaat
     1501 tcgaccagcg cattgcctgg taggtgctga ggcgcaaagg tccagactga accaggacga
     1561 gctgt
//
LOCUS       DAOSIT010000590         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_69363_length_1559_cov_8.079787, whole genome shotgun sequence.
ACCESSION   DAOSIT010000590 DAOSIT010000000
VERSION     DAOSIT010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69363_length_1559_cov_8.079787"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLL14_11280"
     CDS             complement(<1..184)
                     /locus_tag="PLL14_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04555.1"
                     /translation="MSRGVPRLASILFGCLLPIVGLAVVAGVLHGAAWGWAAAALGFL
                     LLVIAQAKRLSVLSAWL"
     gene            complement(181..906)
                     /gene="phoB"
                     /locus_tag="PLL14_11285"
     CDS             complement(181..906)
                     /gene="phoB"
                     /locus_tag="PLL14_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HOG04556.1"
                     /translation="MTRALSTLPLVLVVEDDKGIQELLRFTLVRGGYQPLCADSAEQA
                     ERILRETLPDIALIDWMLPGRSGLALTGTLRHDRRTRALPIILLTARGEEGDRVAGLE
                     GGADDYIVKPFSPKEVLARVQAVLRRCAPEFAKGTLSAGPIALDTVSHEARVDGRRVT
                     LTPTEFRLLRFLMGNPGRVYTRQQLLDNVWGDHVYIEDRTVDIHIRRLRVALGPTAEQ
                     MVETVRGAGYKLGTHSESQHTAQ"
     gene            complement(903..>1559)
                     /gene="phoU"
                     /locus_tag="PLL14_11290"
     CDS             complement(903..>1559)
                     /gene="phoU"
                     /locus_tag="PLL14_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOG04557.1"
                     /translation="IRTRILQMGGLVERQVLAAIDAFSTGNIEEMEEVIRADRDVDAY
                     EVGIDEDCTLLIARRQPTARDLRLVMAISRIVTDLERSGDKAAKIANKARKLHRAGGQ
                     RIPRISDVQHCGRLATDMLQAALDALARMDVDGARQVISADKAIDAAFNAILRQLVTY
                     MMEDPRTIGEALDIIWIAKAIERVGDHATNIAENVIYIVSGVDVRHAGTATHQASKKT
                     "
BASE COUNT          268 a          521 c          488 g          282 t
ORIGIN      
        1 cgagccatgc cgacagcacc gacaggcgct tggcctgcgc gatgaccagc aacagaaaac
       61 ccagcgccgc ggccgcccaa ccccaggcgg ctccatgcag cacaccggcg accaccgcga
      121 ggccaacgat gggcagcaga cagccgaaca gaatgctggc gagcctcggc acgccgcggc
      181 tcattgcgcc gtatgctgtg actcggaatg ggtgcccagc ttgtagccgg cgccacggac
      241 ggtttcgacc atctgctcgg cggtcggccc aagggcgacg cgcaggcgcc ggatgtggat
      301 atcgacggtg cggtcctcga tatacacatg atccccccag acgttgtcca gcagttgctg
      361 gcgcgtatag actcgccccg ggttgcccat caggaagcgc aacagtcgga actcggtcgg
      421 cgtcaaggtg acccgccggc cgtcgacacg cgcttcgtgg ctgaccgtat caagcgcaat
      481 cggaccggcg ctgagcgtgc ccttggcgaa ctccggcgcg cagcggcgca gtactgcctg
      541 cacgcgcgcg agcacttcct tggggctgaa gggtttgacg atgtagtcgt cggcaccgcc
      601 ctcaagcccg gcgacgcgat cgccttcttc gccgcgggcg gtcaacagga tgatcggcag
      661 cgcacgcgta cgccggtcat ggcgcagcgt gccggtcagc gccagacccg atctgccggg
      721 cagcatccag tcgatcagcg cgatgtcggg cagggtctcg cgcaggatcc tttcggcctg
      781 ttcggcgctg tcggcacaga gtggctgata gccgccgcgg accagcgtga agcgcaagag
      841 ttcctgaatg cccttgtcgt cctcgacaac cagcaccagc ggtagcgtgg agagcgcccg
      901 tgtcatgtct tcttgctcgc ctgatgcgta gcggtgccgg cgtgtcgaac atcgacgcca
      961 ctgacgatat agatcacgtt ttctgcaatg ttggtcgcgt gatcgccaac acgctcgatg
     1021 gccttggcaa tccagatgat gtccagcgcc tcgccgatgg tacgtgggtc ttccatcatg
     1081 taggtcacca gttgccgcag gatggcgttg aatgcagcgt cgatcgcctt gtccgcactg
     1141 atgacctggc gggcaccgtc cacatccatg cgcgccagcg cgtcgagcgc agcctgcaac
     1201 atgtcggtcg ccaggcgccc acaatgctgg acatcggaaa ttcttggaat ccgctgaccg
     1261 ccggcccggt gcagtttgcg cgccttgttg gcaatcttcg cagccttgtc gccgctgcgc
     1321 tccaggtccg tgacaatccg cgagatggcc atcaccaggc gcagatcgcg cgccgtaggc
     1381 tgccgacgtg caatcagcag cgtgcagtct tcgtctatgc ccacttcgta ggcgtccacg
     1441 tccctgtcgg ctcggatgac ctcctccatt tcctcgatgt tgccggtact gaaggcgtcg
     1501 attgccgcca gcacctgtct ttcaaccagc ccccccattt gcaggatgcg ggtgcggat
//
LOCUS       DAOSIT010000591         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_69906_length_1551_cov_8.394385, whole genome shotgun sequence.
ACCESSION   DAOSIT010000591 DAOSIT010000000
VERSION     DAOSIT010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69906_length_1551_cov_8.394385"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLL14_11295"
     CDS             <1..214
                     /locus_tag="PLL14_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04558.1"
                     /translation="GEQGHQKSQGGGQDRADARVHALKHLPPGRVPLRRVAQQGASST
                     NIHRTSVRMRREQGRFPRTRTDSALH"
     gene            complement(534..899)
                     /locus_tag="PLL14_11300"
     CDS             complement(534..899)
                     /locus_tag="PLL14_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HOG04559.1"
                     /translation="MVARVIATPAALELIERLKSKHGELMFHQSGGCCDNSAANCYLP
                     TDLTIGAGDVHLGDIGGVPFYMSKSQFAYWKHTQLIIDVIDGHGGTFSLEGPEGKAFH
                     TRSRVFNDAERAELRATDD"
     gene            complement(998..>1551)
                     /locus_tag="PLL14_11305"
     CDS             complement(998..>1551)
                     /locus_tag="PLL14_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG04560.1"
                     /translation="RVEAIKQGNPLDTEAMIGAQASQEQMNKILSYLDLGKQEGATCL
                     IGGARANLGGDLAGGYYIQPTLLKGHNKMRIFQEEIFGPVLAVTTFKDEAEALAIAND
                     TLYGLGAGVWSRNGNVAYRMGRAIKAGRVWTNCYHAYPAHATFGGYKESGIGRETHKV
                     MLDHYQQTKNLLVSYSEQKLGFF"
BASE COUNT          245 a          473 c          522 g          311 t
ORIGIN      
        1 tggcgaacag ggccaccaga aaagccaggg tggcggtcag gaccgggcgg atgcgcgggt
       61 gcatgcgctc aagcacctgc cgcccgggcg cgtgccgttg cggcgcgtcg ctcagcaggg
      121 agcgtcatca acgaacatac atcgaacgtc agtacgcatg cgtcgggagc aggggcggtt
      181 tccccggacc aggaccgatt cggcgttgca ctaggaagca tttccacgag gccgtccgta
      241 agtccacatg cttctccggt cagagccccg ccgaccgggg attccaggcc ggcggttgcc
      301 ggtcgaatgt cctgccggca tgcaagagcg aagccagcaa aaccgttcgc ggtattctac
      361 cctgcgggaa gcatgggtgt ggcggcttct gctacccgta ccttgtgcgg ccatcccgct
      421 ttgccaagtg catggtcaca acggcgctgc gctgcagtgc cagttcatcg ccgccgtggt
      481 ttgggagttt ctatctgcgt tgccgccatg gccgagggca tgggttgctc atctcaatcg
      541 tcggtggccc gaagttcagc gcgctcggcg tcgttgaata cccgcgagcg ggtgtggaag
      601 gccttgcctt ccgggccttc gagcgagaag gtcccgccgt ggccgtcgat gacatcgatg
      661 atcagctgcg tgtgcttcca gtacgcgaat tgcgatttgc tcatgtagaa aggcacgccg
      721 ccgatgtcgc cgaggtgcac atcaccggca ccgatggtca ggtcggtcgg cagatagcag
      781 ttggccgcgc tgttgtcgca gcagccaccc gactgatgga acatcagctc cccgtgtttc
      841 gatttcagac gttcgatcag ttccagcgct gcgggtgtgg cgatgacgcg tgcgaccatg
      901 ctgctctcct cgtctcggga acctccggaa aagcgcgggg cggttgcccg cccctgccgg
      961 gctgcccgcc gtgcgggtca gccacggcgt gcctcgctca gaaaaagccc aacttctgtt
     1021 cgctgtagct gaccagcaga ttcttggttt gctggtagtg gtcgagcatc accttgtggg
     1081 tttcgcggcc gatgccggat tccttgtagc cgccgaaggt ggcgtgcgcc ggataagcgt
     1141 ggtagcagtt ggtccacacg cgtccggcct tgatcgcgcg gcccatgcga taggcgacat
     1201 tgccgttgcg gctccagacg ccggcaccca gaccgtagag cgtatcattg gcgatggcca
     1261 gcgcctcggc ttcgtccttg aaggtggtga ccgcgagcac cgggccgaag atctcttcct
     1321 ggaagatgcg catcttgttg tggcccttga gcagcgtcgg ctggatatag tagccgccgg
     1381 cgaggtcgcc gccgaggttg gcgcgcgcgc cgccgatcag gcaggtggcg ccttcctgct
     1441 tgccgagatc gaggtaggag aggatcttgt tcatctgttc ctgcgatgcc tgggcgccga
     1501 tcatcgcctc ggtgtcgagc gggttgccct gcttgatggc ctcgacgcgg g
//
LOCUS       DAOSIT010000592         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_69984_length_1550_cov_9.757191, whole genome shotgun sequence.
ACCESSION   DAOSIT010000592 DAOSIT010000000
VERSION     DAOSIT010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69984_length_1550_cov_9.757191"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLL14_11310"
     CDS             <1..999
                     /locus_tag="PLL14_11310"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOG04561.1"
                     /translation="DRDPGIRVIGTAHDPIFAIEKMRREWPDVLTLDIEMPRMDGITF
                     LGQLMQERPTPVVICSSLTTAGAETTLQALAAGAVSIITKPGLGVGNFLQDSSDNLVA
                     AVKAAARANMGAVQRGARAPTLAAAPPPRLAMAQTTERLVAIGTSTGGTVALEALLTV
                     LPRVSPGIVIVQHMPEMFTAAFAARLDGLCEISVQEAKHGQRVLPGLALIAPGGKQMQ
                     LARSGAQYVVEVKEGPPVNRHRPSVDVLFRSVAKFAGKNAMGVIMTGMGDDGARGLKE
                     MHDAGAYTLAQDEATCVVYGMPKEAVKLGAVDRSLPLQALAAAILSGAVSSRPRQE"
     gene            complement(1118..1486)
                     /locus_tag="PLL14_11315"
     CDS             complement(1118..1486)
                     /locus_tag="PLL14_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG04562.1"
                     /translation="MIGYVTLGTNDLSAATAFYDALLAETGAKRVWEFPRGVAWGVAP
                     DKPSLAVMTPFDGNAATVGNGVMVALVVDSHEKVDRVHARALELGGKDEGPVGARGEG
                     FYAGYFRDLDGNKLNVFCHG"
BASE COUNT          261 a          497 c          528 g          264 t
ORIGIN      
        1 gaccgcgatc ccgggatccg ggtcatcggc accgcccacg atccgatctt cgcgatcgag
       61 aagatgcgcc gcgaatggcc ggacgtgttg acgctggata tcgagatgcc gcgcatggac
      121 ggcatcacgt ttcttggtca gttgatgcag gaacgcccga cgccggtggt catctgctcg
      181 tcactgacca ccgcgggggc ggaaacgacg ctgcaggccc tggccgccgg cgcggtgtcg
      241 atcatcacca agcccggcct cggcgtcggg aactttctgc aggattcgag tgataacctg
      301 gtagccgccg tcaaggcggc ggcgcgggcc aatatgggcg ccgtgcagcg cggcgcgcgc
      361 gcgccgacgc tggcggctgc gccgccacca cgtctggcga tggcgcagac caccgagcgc
      421 ctggtggcga tcgggacttc gaccggcggc acggtggccc tcgaagcctt gctgaccgta
      481 ttgccgcggg tctcgccggg aatcgtcatc gttcagcaca tgccggagat gtttaccgca
      541 gcctttgccg cgcgtctcga tgggctctgc gagatctcgg tgcaggaagc gaagcacgga
      601 caacgcgtgc tgcccggtct ggcgctgatt gccccggggg gcaagcaaat gcaactggcg
      661 cgcagcggtg cgcagtatgt cgtcgaggtg aaggagggcc cgccggtcaa tcgccatcgc
      721 ccgtcggtcg atgtgttgtt tcgatcggtg gcgaagtttg ccggcaagaa tgcgatgggg
      781 gtgatcatga ccggcatggg cgacgatggc gcgcgcggcc tgaaggagat gcacgacgcc
      841 ggggcctata ccctggccca ggacgaggcg acctgcgtcg tctacggcat gcccaaggaa
      901 gccgtcaaac tcggcgccgt cgaccgcagc ctgccgctgc aggctctcgc cgcagccatc
      961 ttgagtggcg ccgtaagttc acgaccccgt caggaatagc tgcgagcgag cacgctcaga
     1021 ctgccggaaa ccccgcgcgg gacgtcatgg cgccggtgct atcggcggcg ccgcgttccc
     1081 ggcgggtgag gagggaagtg ccatcgccac acgcacgcta gccatggcag aagacgttca
     1141 gcttgttgcc atccaggtcg cgaaagtaac cggcgtagaa accttcgccg cgtgcgccga
     1201 ccgggccttc atccttgccg ccgagttcga gcgccctggc atgaacgcga tcgaccttct
     1261 cgtgcgagtc gaccaccagg gccaccatca cgccgttgcc caccgtggcg gcgttgccgt
     1321 cgaaaggcgt catgacggca agactcggtt tgtctggcgc cactccccag gcgacgccgc
     1381 gcggaaattc ccacacgcgt ttggctcctg tttccgccag caaggcgtca tagaaggcgg
     1441 tggcagcgga caggtcgttg gttccaagcg tgacgtagcc gatcatctga ttctcctttg
     1501 ggggtcaaga gccgccagtt taagtcactt gtcgtcgcgg cggtatcgtc
//
LOCUS       DAOSIT010000593         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_70182_length_1547_cov_5.754021, whole genome shotgun sequence.
ACCESSION   DAOSIT010000593 DAOSIT010000000
VERSION     DAOSIT010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70182_length_1547_cov_5.754021"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLL14_11320"
     CDS             complement(<1..324)
                     /locus_tag="PLL14_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG04563.1"
                     /translation="MIPLSLLIQNIGPHGDTRVDFRDLPSPLAVVGEYGSGKTYLIEG
                     IFAAIYGEFAFYPSSIYDALTLDAPEDGLIHLWFEHDGETFQAEQKIKRSRKREATIR
                     DASGNL"
     gene            complement(321..548)
                     /locus_tag="PLL14_11325"
     CDS             complement(321..548)
                     /locus_tag="PLL14_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04564.1"
                     /translation="MDTMKLRVRFGAMCEGIDEQVRQQGFMLPDKDRGTFQKCADAAT
                     LLSVQGYATDAETVKIRKRIMKAIGKAAVAS"
     gene            complement(586..>1547)
                     /locus_tag="PLL14_11330"
     CDS             complement(586..>1547)
                     /locus_tag="PLL14_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04565.1"
                     /translation="LMIPGNHDYSGPGRADALHVFDACENVTVARGVGFGGPVGVRVL
                     CVPWLYTDKSFDEVVLDAASVPCDLLLAHVQVRGALQNRRHAMEFGQPMKPYEPRPGD
                     WSTTREFLASLPVRHFALGDFHMRQDLTDGRGGYVGALRQLDFGEEGNPAGFEIWDSE
                     SNLTEWIELDECPQHRTIIARPGESVALLDDCIKYRVRYDGAPDPLEARELEAQGVQV
                     EAIVERRERVKRCNVPSGMVGDLPATLRFWAEQQTDEVDVDRALAALKLIDSDDLVPA
                     EAMDEQAQTMAEAGVEGVTQGVTAAVDQAFAATGTDDDESLPF"
BASE COUNT          275 a          512 c          467 g          293 t
ORIGIN      
        1 caggttgccg ctcgcgtcgc ggatcgtcgc ctcgcgcttc cttgagcgct tgatcttctg
       61 ctcggcctgg aaggtttccc cgtcatgctc gaaccagaga tggatcagcc cgtcctccgg
      121 cgcgtccagc gtgagcgcgt catagatcga tgacggatag aacgcgaact cgccgtagat
      181 cgccgcgaag atcccctcaa tgaggtaggt tttgcctgac ccgtattcgc cgacaacggc
      241 cagcgggctg ggcaggtcgc ggaaatcgac ccgcgtgtcg ccgtgcggac cgatgttttg
      301 gatgagaagg ctcaggggga tcatgaggcc accgccgctt tgccaatggc cttcatgatg
      361 cgcttgcgaa ttttgacggt ttcggcgtcg gtcgcatacc cctgcaccga gagcagggtt
      421 gcggcgtcgg cgcatttctg aaaagttcca cgatctttgt ccggcaacat aaagccctgc
      481 tggcgaacct gctcgtcgat gccttcacac attgcgccaa agcgcaccct cagcttcatt
      541 gtgtccatct ttcgctcctc cagaatttcc aacagaaacg tgtgatcaga acggcaacga
      601 ttcgtcgtcg tccgtgccgg tggccgcgaa agcctgatcg accgcagcag taaccccttg
      661 ggttacacct tcgacgccgg cctcggccat cgtctgcgcc tgctcgtcca tcgcctcagc
      721 cggcacaagg tcgtcgctgt caatcagctt gagtgccgcc agcgcccggt caacgtccac
      781 ctcgtccgtc tgctgctcgg cccagaaccg gagcgtcgcg ggcagatccc cgaccatgcc
      841 actcggcacg ttgcagcgct tgacccgctc gcggcgctcg acaatggcct cgacctggac
      901 gccctgcgct tcgagttcgc gcgcttccag cgggtcgggc gcgccgtcat agcgcacgcg
      961 gtatttgatg cagtcatcca ggagcgcaac cgattcgccg ggccgcgcga tgatcgtccg
     1021 gtgctgcggg cattcgtcca gttcgatcca ctcggtcagg ttgctctcgg aatcccaaat
     1081 ctcaaagccg gcggggttgc cctcttcgcc gaaatcgagt tgcctgagag cgccgacata
     1141 tccgccgcga ccgtccgtca gatcctggcg catgtggaag tcacccaaag cgaagtgacg
     1201 cacaggcagg ctggcgagaa actcgcgcgt cgttgaccag tcgcccgggc gcggctcgta
     1261 gggcttcatc ggctggccga actccatggc atgccgccga ttttgcaggg cgccacgcac
     1321 ctggacatgg gcgagcagga gatcgcacgg aacgctggcg gcatctagga cgacctcatc
     1381 gaatgacttg tcggtgtaga gccacgggac acagaggacg cggacgccaa cgggaccgcc
     1441 aaatccgacc ccgcgtgcca cagtcacgtt ctcgcaggcg tcaaacacat ggagcgcgtc
     1501 ggcccggccc ggcccgctgt agtcgtgatt gccgggaatc atcagcg
//
LOCUS       DAOSIT010000594         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_70724_length_1539_cov_7.411051, whole genome shotgun sequence.
ACCESSION   DAOSIT010000594 DAOSIT010000000
VERSION     DAOSIT010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70724_length_1539_cov_7.411051"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLL14_11335"
     CDS             <1..673
                     /locus_tag="PLL14_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04566.1"
                     /translation="PAAGDLQRLALWGGVVWFLFVAVALYRIKLLGRYYIAPTWLFTV
                     AAAASLFTHRIATRHARRLWLAATCVVAANFIGIVLQNNNPRLVERELAAFAATSSEP
                     VFTDPYTASYAAEFLHWAKIPPGRVVGSPPAEGALYFVVPTRIDAPNRLTPASMLPAL
                     QVKPEWVVVRRIDGPAALPSFLVPILRSAPLIPAWIKAKLTEPRGNLLVVRAVRLGST
                     PEKSP"
     gene            670..1026
                     /locus_tag="PLL14_11340"
     CDS             670..1026
                     /locus_tag="PLL14_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG04567.1"
                     /translation="MTPTLVVMLVLYSVMLASGQILFKMAAEAAREEGGSFFVALLFE
                     PKFILAVALYGLLTLLWTWILSQVPLSRAYPFVALAFVVTPVMGSLLLGERLSGTVIL
                     GTGLVLAGLMVVVYAE"
     gene            1016..>1539
                     /locus_tag="PLL14_11345"
     CDS             1016..>1539
                     /locus_tag="PLL14_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG04568.1"
                     /translation="MPNEDIRSSVKVLPEVLSAVQGAGIAAVIPCFRVHDKVLGVIAR
                     IGSEVERIYVVDDACPEHTADLVQRVCRDPRVAVIRLPANQGVGGAVVAGYRRALSDG
                     AQIVVKIDGDGQMAPELVPKLVAPILAGHADYVKGNRFFFLDGVRQMPLVRLVGNAGL
                     SFLTKLSTGYWQIF"
BASE COUNT          248 a          488 c          477 g          326 t
ORIGIN      
        1 ccctgccgct ggcgacttgc agcgactggc gctctggggt ggggtggtat ggttcctgtt
       61 cgtagcggtg gcactgtatc gcatcaagct gctggggcgc tactacatcg ctccgacatg
      121 gctgttcacg gtcgccgcgg ccgcctcgct gttcacccat cggatcgcga cgcgccatgc
      181 ccgccgactc tggctggcag caacctgcgt cgttgccgcc aatttcatcg gtatcgtgct
      241 gcagaacaat aacccccggc tcgtggaaag ggaactcgca gcttttgcgg caacttcctc
      301 cgagccggta tttacagatc cctacacggc cagctatgcc gcagaattcc tgcattgggc
      361 gaagattccg ccgggtcgcg tcgtgggctc cccgccggct gagggcgcgc tttacttcgt
      421 cgtacccaca cgcatcgacg cccccaatcg actgacgccg gcatcgatgc tgcctgcact
      481 ccaggtcaag ccggaatggg tggtggttcg ccggattgac ggaccggcag ccctgccgtc
      541 gttcctggtc ccgatactgc gcagcgcgcc actcattccc gcatggatca aggccaaatt
      601 aaccgagcca cgcggcaacc tgctggtcgt tcgcgcggtg cgacttggct ccactccgga
      661 gaaatcccca tgacgcccac gctagtggtc atgctcgtgc tctactcggt gatgctcgct
      721 tccggccaga tcctgttcaa gatggcggct gaagcggcac gcgaggaagg cggcagcttc
      781 tttgtggcgc tgcttttcga gccgaaattc attctcgccg tagcgctcta cggacttctg
      841 actttgctat ggacgtggat cctctcgcag gtaccgcttt cacgtgccta cccgttcgtc
      901 gcactcgcgt tcgtcgtcac tccggtgatg ggaagcctgc tgctcggcga gcggctctcc
      961 ggcaccgtca tcctgggaac aggccttgtc ctggctggcc tgatggtggt tgtctatgcc
     1021 gaatgaagac atccggagca gcgtcaaggt gctgcctgaa gtgctgtcgg ctgtgcaagg
     1081 cgccggcatc gccgcggtaa tcccgtgttt ccgggtgcac gacaaggtcc tcggggtcat
     1141 cgccaggatc gggagcgagg tggagcgcat ttatgtggtc gacgacgcct gcccggagca
     1201 tacggcagac ctcgtgcagc gcgtctgccg cgaccccagg gtcgcagtca ttcgcctgcc
     1261 ggcaaaccag ggcgtcggtg gcgcggtcgt cgcaggatat cggcgcgcgt taagcgacgg
     1321 cgcgcagatc gtggtcaaga ttgacgggga tggtcaaatg gccccggaac tcgtcccgaa
     1381 actggttgct ccgatccttg ccgggcatgc cgactatgtc aagggcaacc ggtttttctt
     1441 ccttgacgga gtccggcaaa tgcctctggt caggctggtc ggcaacgctg gactgtcctt
     1501 tctgaccaaa ctgtcgaccg gctactggca gatattcga
//
LOCUS       DAOSIT010000595         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_70836_length_1537_cov_8.719973, whole genome shotgun sequence.
ACCESSION   DAOSIT010000595 DAOSIT010000000
VERSION     DAOSIT010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70836_length_1537_cov_8.719973"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLL14_11350"
     CDS             <1..450
                     /locus_tag="PLL14_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOG04569.1"
                     /translation="VTSALQMHAAVHHEVAASDVFIAVAAVADYRPAKPIGHKIKKGA
                     QAAPPQIELLQNPDILAEVAALRDPPLCVGFAAESENLAEYAENKRRSKKIPLLVGNL
                     IHDGFGGDDNTLILFDDEGQHPLPSAPKLELARQLVARIASLLEKRR"
     gene            447..908
                     /gene="dut"
                     /locus_tag="PLL14_11355"
     CDS             447..908
                     /gene="dut"
                     /locus_tag="PLL14_11355"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG04570.1"
                     /translation="MSRPDLRVDVKLLDPRLRDSPPHYATAGSAGLDLRACIAAPADI
                     RPGETLLIPTGMAIHLADPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGEIMVST
                     WNRGLQTFTLQPLERLAQLVIVPVLQMRFNVVEDFAASARAAGGFGSTGQG"
     gene            complement(930..1418)
                     /locus_tag="PLL14_11360"
     CDS             complement(930..1418)
                     /locus_tag="PLL14_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOG04571.1"
                     /translation="MTLSLRTAFALLAMAAVGLTLAGWAIGELMKLQPCPLCIFQRLL
                     YLLIGLLALAGVLFPGARRLWGALLALTAAGGVATAAYQSWLQYLPEVSRECGFGEPT
                     VIERIVDWFGVRWPAMFMATGFCSSKEWVFLGLSMANWSVLCFLVFLAAAVWLLLRRI
                     ER"
BASE COUNT          297 a          528 c          501 g          211 t
ORIGIN      
        1 gtgaccagcg cgctgcagat gcacgcggcg gtccaccacg aggtcgcggc gagcgacgtg
       61 ttcatcgccg tcgcggcggt ggccgactac cgacccgcga agccgatcgg ccacaagatc
      121 aagaaaggcg cccaggccgc gccaccgcag atcgaactgc tgcagaatcc ggacatcctc
      181 gccgaagtgg ccgcgctgcg cgatccgccg ctgtgtgtcg gttttgctgc cgagagcgag
      241 aatctggcgg aatacgcgga aaacaaacgg cggagcaaga agattcccct gctggtcggc
      301 aacctgatcc acgacggttt tggtggcgac gacaacacgc tgatcctgtt cgacgacgag
      361 ggccagcacc ccctgccttc ggcgcccaaa cttgaacttg cccgtcaact cgtcgcgcgc
      421 atcgccagcc tgctcgagaa acgccgatga gccgaccgga cctgcgggtg gacgtgaagc
      481 tgctcgaccc gcgcctgcgt gacagcccgc cgcactatgc gactgccgga tcggccggtc
      541 tcgacctgcg cgcgtgcatc gcggcgccgg cggacatccg gcccggggaa acgctgctga
      601 ttccgaccgg gatggccatt cacctcgccg acccggggct ggcggcgatg atcctgccgc
      661 gctcggggct aggccacaaa cacggcatcg tcctgggcaa cctggtgggc ttgatcgact
      721 ccgactatca gggcgaaatc atggtctcga cctggaaccg tggtctgcag accttcacgc
      781 tgcagccgct cgaacgcctg gcgcaactgg tgatcgtgcc ggtgttgcag atgcgcttca
      841 acgtggtcga ggacttcgcg gccagcgcgc gcgccgcggg tggtttcggc agcaccggtc
      901 agggctgagg ctgaggcagc gccgaacgtt cagcgctcga tgcgccgcag cagcagccat
      961 accgcggcgg ccagaaagac gagaaaacaa agcaccgacc agttggccat cgacaggccg
     1021 aggaacaccc attccttgct cgaacagaaa ccggtggcca tgaacatcgc tggccagcgc
     1081 acgccgaacc agtcgacgat gcgttcgatc acggtcggtt cgccgaaacc gcactccctg
     1141 ctgacctcgg gcaggtattg cagccagctc tggtaggcgg cagtggccac gccgccggcc
     1201 gcggtcaagg ccagcagggc gccccacagg cggcgcgcgc cgggaaacaa gacgccggcc
     1261 agcgccagca ggccgatcag cagatagagc agccgctgaa aaatgcacag cggacacggc
     1321 tgcagtttca tcagttcgcc gatcgcccag ccggccagcg tcagacccac ggccgccatc
     1381 gccagcaggg cgaaggcggt ccgcaaggaa agcgtcatcg gcgcgccgct caggccagca
     1441 cgtagctgcg caagcgcagc gacagctcct gcaactgccg gacgccggaa ttctcggcgc
     1501 gctcgcacca gtcctgcagg gcctggagca gatgctc
//
LOCUS       DAOSIT010000596         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_70840_length_1537_cov_6.452767, whole genome shotgun sequence.
ACCESSION   DAOSIT010000596 DAOSIT010000000
VERSION     DAOSIT010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70840_length_1537_cov_6.452767"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLL14_11365"
     CDS             <1..608
                     /locus_tag="PLL14_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOG04572.1"
                     /translation="TRNWDVFLAETLPVLAEAFAGGGEVERLSAYALRRSASSRRATG
                     KALGTADYSRLLIDFSAALLALPEADGGPLGTFVPRCLKKSAKRVKERAADALGASVA
                     ARHRLRVAFKQLRYALEFFTPILEGPVLANYHHSASCLQDLLGRLNDLAVAEQLVAEA
                     LPGSRGKRLEGWLAAQTESLLPEFSRALIDFQQQREPWQAA"
     gene            complement(714..>1537)
                     /gene="mgtE"
                     /locus_tag="PLL14_11370"
     CDS             complement(714..>1537)
                     /gene="mgtE"
                     /locus_tag="PLL14_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOG04573.1"
                     /translation="EGGRLVGVVSLRQLLLTRSGTPLHKVMNRRVMKVTTDADQTTVA
                     MLVDKYRLLAIPVVDEDNVLVGMVTVDDVIDVIEEETTRDMLKMAGTSESETLTHSAF
                     KIAGIRLPWLLAAFIGGLAATAVIGQYEAILGEVLVLSAFLPVVMGMAGNVGVQSATV
                     AVRGLATGAIDVRDTVPLVLKELRVGLVLGGFYGVILAIYGYLVHHSLQLGQVVGLTI
                     LGNMTGAALLAVLLPMIFQRLKVDPAVATGPFVTTAIDILGVLNYFVIASWIYKL"
BASE COUNT          286 a          482 c          489 g          280 t
ORIGIN      
        1 atactcgtaa ctgggatgtt tttcttgcgg aaaccttgcc ggtgctggcc gaggcatttg
       61 ctggtggcgg cgaggtcgag cggttgtccg cctacgccct tcgccgctcg gcgagcagtc
      121 gcagagccac cggcaaagcg ctcggcacgg cagactactc gcggttgctg atcgacttct
      181 cggccgcgct cctggccttg ccggaagccg atggcgggcc gctcggaacg ttcgtcccgc
      241 gttgcctgaa gaaatccgcg aagcgggtca aggagcgcgc tgcggacgcg ttgggcgcca
      301 gcgttgccgc ccgtcaccgc ttgcgcgtgg ccttcaagca attgcgctac gcgcttgaat
      361 tttttacccc gatccttgaa ggacccgtgc tggccaacta ccatcactcg gcaagttgtc
      421 tgcaggatct gctgggccgt ctgaacgacc tggcggtcgc cgagcagctt gttgcggagg
      481 cgttgcctgg cagcaggggc aaacggctag aaggctggct ggcggcacag accgaatcct
      541 tgctgcctga attcagcagg gcgttgattg acttccagca gcagcgagag ccttggcagg
      601 cggcgtaaga ttgcaggtga cggcaccagt cccggttacg agactggtgg cccgcggagt
      661 gcggtgttgc tgaacgggac gccgccggcg gtccaggccc ggtggcgaca ccgtcacaac
      721 ttgtagatcc aactggcgat cacgaagtag ttgaggacgc ccagaatgtc gatggcggtg
      781 gtcacgaacg ggcctgtggc gaccgccggg tctaccttca ggcgttgaaa gatcatcggc
      841 agcagtaccg cgagtaacgc cgcgccggtc atgttgccga ggatcgtcag cccgacaacc
      901 tggccgagct gcaggctgtg gtgtaccagg taaccgtaaa tggccaggat cacgccgtag
      961 aaaccgccaa gaaccaggcc gacgcgcaat tccttgagca ccagcggtac ggtatccctg
     1021 acgtcgatcg cacccgtggc gagaccgcgc acggccacag ttgcggactg cacgccgacg
     1081 ttgccggcca tgcccatgac caccggcagg aacgcactga gcaccaggac ttcgccaaga
     1141 atcgcctcgt attgcccgat caccgccgtc gctgccagac cgccaatgaa cgccgccagc
     1201 agccagggca aacggattcc ggcgatcttg aatgccgagt gggtgagcgt ctccgattcg
     1261 ctggtgcccg ccatcttcag catgtcgcgg gttgtttcct cctcgatgac atcgatgacg
     1321 tcatcgaccg tgaccatgcc gaccagcaca ttgtcttcgt ccaccaccgg gatggcgagc
     1381 aggcgatact tgtcgaccaa catggcaacg gtggtttggt cggcgtcggt agtgaccttc
     1441 atcacgcgcc ggttcatgac cttatgcagt ggtgtaccgg aacgggtcag cagcaactgt
     1501 cgcaaggaca cgacacccac caggcgcccg ccctcgt
//
LOCUS       DAOSIT010000597         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_70889_length_1536_cov_6.376097, whole genome shotgun sequence.
ACCESSION   DAOSIT010000597 DAOSIT010000000
VERSION     DAOSIT010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70889_length_1536_cov_6.376097"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="trkA"
                     /locus_tag="PLL14_11375"
     CDS             <1..1127
                     /gene="trkA"
                     /locus_tag="PLL14_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOG04574.1"
                     /translation="DFLDSEKLLSTANFAVDYALCPEQVITEYIARLVDFPEALQVLS
                     FGGGRLALIAVRAYEGGLLVGNAIKQMRTHLPPEVDGRIAAIYRRDGAIMPSGETVIE
                     DGDEVFLLAAEEHIRTVMRELRRSVEPVRRVMIAGGGNIGLRVAQALESRCEVKTIEV
                     DRRRAEFVATTLKSVLVLCGDATDEELLQQEAIDEMDLFLALTNDDEDNIMAASLAKR
                     MGCKRVVALINRRAYAELVQGGPIDIALSPAQVSIGTLLTYVRRGDVAQVHSLRRGAA
                     EALEIVAHGDQKTSKVVGRRIGDIPSIPGAFIAAVVRDLDKSQDLGFLGLAKKKQAGR
                     VLIAHRDVLIESGDHLIVFCLDKKVVKQVEKLFAVGFHFF"
     gene            1140..>1536
                     /locus_tag="PLL14_11380"
     CDS             1140..>1536
                     /locus_tag="PLL14_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOG04575.1"
                     /translation="MQRYYPVVRVLSLVILMFGLTMLVPWGLSWALHDGAESAFDEAV
                     LLTVGTGLGLWYATRKEKRDLTIRDGFLMVALVWTVLPVYAGLPLIIQMGASFTDAYF
                     EAVSGLTTTGATVFEGLDALPVSINFWRCQ"
BASE COUNT          264 a          441 c          517 g          314 t
ORIGIN      
        1 gcgacttcct ggacagtgaa aaactgctgt cgacagcgaa tttcgcggtg gattatgctt
       61 tgtgcccgga acaggtcatc accgagtaca tcgcacgcct ggtcgatttc ccggaagccc
      121 tgcaggtgct gagttttggc ggcggacgcc tggctttgat cgccgtccgc gcctacgaag
      181 gtggtttgct ggtcggcaat gcaatcaaac aaatgcgcac gcacttgcca cccgaggtcg
      241 acggccggat cgccgcgatc tatcgccgcg acggggcgat catgcccagt ggcgagacag
      301 tgatcgaaga cggtgacgag gtcttcctgc tcgcggccga ggagcatatc cgcaccgtga
      361 tgcgcgagtt gcgacgctcg gtcgagccgg tacgccgggt gatgatcgcc ggtggcggca
      421 atatcggatt gcgcgtggcg caggctctgg agagcagatg cgaagtcaag accatcgagg
      481 tcgaccggcg gcgtgccgaa ttcgtggcta ccaccctgaa gtcggtactg gtactctgcg
      541 gcgacgccac cgacgaggag ttgttgcagc aggaggccat cgacgagatg gacctcttcc
      601 tggcgctgac caatgacgac gaagacaaca tcatggccgc gtcgctggcc aagcgaatgg
      661 gttgcaagcg ggtggtggcg ttgatcaatc gccgcgccta cgccgaactg gtccagggcg
      721 gcccgatcga catcgcgctg tcgccggctc aggtatcgat cggtacgctg ttgacctacg
      781 ttcgtcgcgg tgatgtcgcg caggttcaca gcctgcgccg gggggctgcc gaggcgcttg
      841 agatcgtcgc ccatggtgat cagaagacct ccaaggtggt tggtcggcgt atcggtgaca
      901 tcccgtcgat tccaggtgca ttcatcgcgg cggtcgtccg cgacctcgac aagagccagg
      961 atctgggctt tctcggactg gccaagaaga agcaggcggg tcgcgtgctg atcgcccaca
     1021 gggatgtgct gatcgagtcc ggcgaccacc tgatcgtctt ctgtctcgac aagaaggtcg
     1081 tcaagcaggt cgaaaagctg ttcgctgtcg gcttccattt cttctgagag ggctgcgcca
     1141 tgcaacgcta ctacccggta gtgcgggtcc tctcgctggt gatcctgatg ttcggtctga
     1201 cgatgctggt tccctggggt ctgtcctggg cgctgcatga tggtgccgag agcgccttcg
     1261 atgaagcggt tctgctgacc gtcggtaccg gcctggggtt gtggtacgcg acccgcaagg
     1321 agaagcgcga cctgacgatt cgcgatgggt tcctgatggt cgcgctggtg tggaccgtgc
     1381 tgccggtcta cgccgggctg ccgttgatca tccagatggg cgcgagtttc accgatgcct
     1441 atttcgaagc tgtgtccggc ctgacgacga ccggcgcgac cgtattcgag ggcctggatg
     1501 cgctgccggt gtcaatcaac ttctggcgtt gccagc
//
LOCUS       DAOSIT010000598         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71193_length_1532_cov_12.633717, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000598 DAOSIT010000000
VERSION     DAOSIT010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71193_length_1532_cov_12.633717"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /locus_tag="PLL14_11385"
     CDS             complement(<1..>1532)
                     /locus_tag="PLL14_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOG04576.1"
                     /translation="FLWPCQGIRPPGDTYLHLYAADLARSPDGRWWVIADRTQAPSGA
                     GYALENRTIVGRVFPEQFRDLHVQHLASFFRQLQDSLAHWAPRTGEAPLIVLLTPGPY
                     NETYFEHAYLARYLGFPLVEGQDLTVRGDCVYLRTLSGLRRVHALLRRLDDDYCDPLE
                     LRGDSALGIPGLLQAVRAGNVLVANALGSGLIESAALPGFLPGACRKLLGEPLAMPSV
                     ATWWCGERAALAFTIEHLDELVIKGSYPSQRFDPVFGNELHGSKREEMIARLRARPQA
                     YVAQELVNFSEAPAWSSSHARRLLPRGVGLRVFVAATPSGHVVMPGGLTRVSAASNAR
                     VISMQRGGSSKDTWVLTDAAVNTFSLLKQSVGKADIVRGGRNLSSRVVENLYWFGRYA
                     ERCDKTSRLLRVALSRLVDAGADTLPALNSALDLCRTMQLLPTAEAEANAAAKARKAA
                     AALPLDAQMIHAVCSREWGEGLAGDIRRLLWVATQVRERFSLDNWHAINRLQHQLQQY
                     SET"
BASE COUNT          271 a          480 c          540 g          241 t
ORIGIN      
        1 gtctcgctgt actgctgcag ttgatgctgc agacgattga tcgcatgcca gttgtcgagc
       61 gagaagcgct cgcgcacctg tgtcgccacc cacaacaggc gacggatgtc gccggcgagt
      121 ccttcgcccc attccctgct gcaaacggcg tgaatcatct gtgcatccag gggcagtgcc
      181 gcggcggctt tccttgcctt tgcagcggcg ttggcttcgg cctcggcggt cggcagcagt
      241 tgcatggtac ggcagaggtc gagggccgag ttgagtgccg gcagcgtatc ggcgccagca
      301 tcgaccaggc gcgacagggc gacgcgcagg aggcgcgaag tcttgtcaca tcgttccgca
      361 tagcggccga accagtacag gttttcgacg actcgcgacg acaggttgcg gccgccgcgc
      421 acgatgtcgg ccttgccgac cgattgcttg agcaggctga aggtgttgac cgcggcgtcg
      481 gtgagcaccc aggtgtcctt gctcgacccg ccgcgttgca tcgagatgac ccgtgcattc
      541 gaagccgcgg aaacgcgcgt caggccgccg ggcatcacta cgtggccgct gggggtggcc
      601 gcgacaaaga cgcgcaggcc gacgccgcga ggcagcaggc gccgcgcgtg actggagctc
      661 cacgccggcg cttcggaaaa attcaccaac tcctgtgcga cataggcctg cggccgggcc
      721 cgcaggcggg cgatcatctc ctcgcgtttg ctgccgtgca gttcgttgcc gaaaaccgga
      781 tcgaagcgtt gcgaagggta cgagcccttg atgaccagtt cgtcgaggtg ttcgatggtg
      841 aacgccagcg cggcccgctc gccgcaccac caggtcgcta ccgaaggcat tgccagcggc
      901 tcgccgagca gcttcctgca ggcgccgggc aggaatcccg gcagtgcggc agactcgatc
      961 aggccactgc cgagggcatt ggcgaccagg acattgccgg cgcgcacggc ctgcagcagg
     1021 cccggaatgc cgagcgccga atcgccgcgc agttcgagcg gatcgcagta gtcgtcatcc
     1081 agccggcgga gcagcgcatg cacacgccgc aagccgctga gcgttcggag atagacgcaa
     1141 tcgccgcgca ccgtcaggtc ctgtccctcg accaggggaa aaccgaggta gcgcgccaga
     1201 taggcgtgtt cgaagtaggt ttcgttgtag gggcccggcg tcagcaggac gatcagcggc
     1261 gcttcgccgg tacgcggggc ccagtgggcg agactgtcct gaagttggcg gaagaacgag
     1321 gcgaggtgct ggacgtgcag gtcgcggaac tgctcgggaa aaacgcggcc aacgatggtc
     1381 cggttttcga gcgcatagcc ggcacccgat ggggcctgcg tgcggtcggc aatcacccac
     1441 cagcggccgt cgggcgaacg cgcgaggtcg gcggcgtaga gatgcagata ggtatcaccg
     1501 ggcggtctga tcccctggca gggccagagg aa
//
LOCUS       DAOSIT010000599         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71389_length_1529_cov_7.416554, whole genome shotgun sequence.
ACCESSION   DAOSIT010000599 DAOSIT010000000
VERSION     DAOSIT010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71389_length_1529_cov_7.416554"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLL14_11390"
     CDS             complement(<1..472)
                     /locus_tag="PLL14_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbI"
                     /protein_id="HOG04577.1"
                     /translation="MVDQVTEDQMSPEASPGELSRKSGVRRVNNLPVYLVGGVMTVFL
                     AIMVLVAADRNAQQNKPNGGPKEKGGNTSLFASEIAGDQKDGIIPPAAAKPLQISELS
                     APATADGAVLIARPDNLDTPPKPPTSAGGGTPQSGSRDEELNRIRMAKMQQLEEA"
     gene            complement(479..940)
                     /locus_tag="PLL14_11395"
     CDS             complement(479..940)
                     /locus_tag="PLL14_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HOG04578.1"
                     /translation="MRQFALSALLVMALAGCASTAPYGNFATEPPAGLEERIATDTVK
                     QLVTLYPPARTRFHLGQPTPDAYGSALVEALRAKGYALVEFKPGTAPQAGSPEAAAAT
                     APGLNLHYVLDTAASPNLYRITVMVGQQSLSRAYLAQNDRVAPAGAWVRKE"
     gene            complement(943..>1529)
                     /gene="trbG"
                     /locus_tag="PLL14_11400"
     CDS             complement(943..>1529)
                     /gene="trbG"
                     /locus_tag="PLL14_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HOG04579.1"
                     /translation="LGDTARWTVEPAVTGSGAGEVQHLIIKPLDVGLETSMVVTTNRR
                     TYHLRLRSHRSQYMPQVAFTYPEEAMAKWEAIKAREGRDRDDRTLPETREYMGNLSFD
                     YEVSGSAAWKPVRVYNDGRKTIIQMPSTMAQTEAPALLVVRKDGGVFTDDETVMVNYR
                     VQGDRYIVDSVFDKAILIAGVGSSQDRVTIQRGK"
BASE COUNT          225 a          498 c          500 g          306 t
ORIGIN      
        1 ccgcctcttc gagctgctgc atcttcgcca tacggatgcg attcagttcc tcgtcgcggg
       61 agccgctttg cggcgtgcca ccaccggccg aggtcggtgg cttcggcggg gtatccaggt
      121 tgtccgggcg tgcaatcagc acggcgccgt ccgcggtcgc gggcgcagag agttcggaga
      181 tttgcagcgg tttcgcggcc gccggcggga tgattccatc cttttgatcg ccggcgattt
      241 cgctggcgaa caggctggta ttgccgccct tctccttcgg cccgccgttg ggcttgttct
      301 gctgggcatt acggtccgca gcgaccagta ccatgatggc caggaacacg gtcatgacac
      361 cgccgacgag atacaccggc aggttattga cccggcgcac gccggatttt ctcgagagtt
      421 cgccgggcga cgcctcgggg gacatctggt cttcggtcac ttggtcaacc atcgccggtc
      481 actccttgcg aacccaggca ccggccggcg ccacgcggtc attctgtgcc aggtaggcgc
      541 ggctgagcga ttgctgcccg accatcacgg tgatccggta aaggttcgga ctggccgctg
      601 tgtcgagcac gtagtgcagg ttcagccctg gcgcagtcgc cgctgcggct tccggagagc
      661 ctgcctgcgg ggccgttccc ggcttgaact ccacgagcgc atagcccttc gcccgcagtg
      721 cttccaccag ggcgctgcca taggcatccg gcgtcggctg gccaagatgg aagcgcgttc
      781 ttgccggcgg gtacagggtg accagctgct tgaccgtgtc ggtggcaatc ctttcctcca
      841 ggccggctgg gggctcggtc gcgaagttgc cgtagggcgc ggtcgacgca caaccggcca
      901 gagccatcac caggagcgcg gagagggcga actggcgcat ggctatttcc ccctttggat
      961 tgtcacgcga tcctgcgacg agccgacgcc ggcaatcagg attgccttgt cgaacacgct
     1021 atcgacgatg taacggtcgc cctgaacgcg gtaattgacc atcacggttt catcgtcggt
     1081 gaacacgccg ccgtccttgc gcaccaccag cagcgccggc gcttcggtct gggccatcgt
     1141 cgacggcatc tggatgatgg tcttgcgacc atcgttatag acccgcaccg gcttccaggc
     1201 cgcgctgccc gatacctcat agtcgaagct gaggttgccc atgtactcgc gggtttccgg
     1261 cagcgtccgg tcgtcgcgat cccttccttc gcgcgccttg atcgcttccc acttcgccat
     1321 cgcctcctcg gggtaggtga aggccacctg cggcatgtac tggctacgat gcgagcgtag
     1381 ccgcaggtga tacgtgcggc ggttggtggt cacgaccatc gacgtttcca ggccgacgtc
     1441 gagcggcttg atgatcaggt gctgcacttc gccggcgccg ctgccggtga cggccggttc
     1501 gaccgtccag cgggcggtat cgccgaggt
//
LOCUS       DAOSIT010000600         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71475_length_1528_cov_3.463001, whole genome shotgun sequence.
ACCESSION   DAOSIT010000600 DAOSIT010000000
VERSION     DAOSIT010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71475_length_1528_cov_3.463001"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLL14_11405"
     CDS             <1..410
                     /locus_tag="PLL14_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOG04580.1"
                     /translation="GASGFASLNSKDGPWVWKDSYVFAFDCRLDRMVAHPMRPDLVGR
                     SIMQITDNNGKFLFKELCKVGSQPRGGWVEYVWTRPGAGRVSRKLSYALTANIAFSTG
                     IQVAAGVYDEKLTLDNQNTLTEKMADPAKYPAH"
     gene            complement(512..1282)
                     /locus_tag="PLL14_11410"
     CDS             complement(512..1282)
                     /locus_tag="PLL14_11410"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOG04581.1"
                     /translation="MCQLLGMNCNVPTDICFSFTGFQARGGVTDVHRDGWGIAFFEGR
                     GVRVFLDPQPSCESPVAELVRHFPIHSLNVIAHIRKATQGAVGLENTHPFMRELWGRY
                     WIFAHNGNLEDYAPALDGSFLPVGQTDSELTFCHLLQTLRLRFPEAPPDRLVLREALE
                     EFAARVRPHGPFNFMLSNGDCLFAHRSTELHYIVRQAPFTVAHLKDQDVAVDFSELTT
                     PDDRVALIATLPLTDNETWTPLPVDRVAMFAEGSYHPA"
     gene            1326..>1528
                     /locus_tag="PLL14_11415"
     CDS             1326..>1528
                     /locus_tag="PLL14_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04582.1"
                     /translation="MKIAKLFYPGNACQAGRCTINTPDLRFSDASSLSDLTKSAAKSG
                     KTAHFPPYFCANRTTIRLKSVKN"
BASE COUNT          326 a          430 c          478 g          294 t
ORIGIN      
        1 agggggcgtc aggttttgct tcgctgaaca gcaaggacgg tccctgggtc tggaaggaca
       61 gctatgtctt cgcctttgac tgtcgccttg accggatggt cgcccatccg atgcgacccg
      121 acctggtggg tcgctctatc atgcagatca ccgacaacaa cggcaaattc ctcttcaagg
      181 agttgtgcaa ggtcggttct cagccccgtg gcggctgggt cgagtacgta tggacgcggc
      241 cgggggccgg acgcgtatcg cgcaagctgt cctatgcgct gaccgccaat atcgctttct
      301 cgaccggaat ccaggtcgcc gccggggtct atgacgaaaa actcactctt gacaaccaga
      361 acacgcttac cgagaagatg gctgacccag ccaaataccc ggcccactaa gtcatcgccg
      421 gtacgcattg ccgggcttcg ggaaagtgtt gtaacgggac ccggcattgt cttccgacgc
      481 agcagcgcgg cgggagacag atcaagctcg atcaggcggg gtgatacgaa ccctcggcga
      541 acatcgccac gcgatcgacc ggtagcggcg tccaggtttc gttgtcggtc agtggcaggg
      601 tggcgatcag tgccacgcgg tcgtccggtg tcgtcaactc gctgaaatcg accgccacgt
      661 cctgatcctt gaggtgggcg acggtgaacg gcgcctggcg gacgatgtag tgtaattcgg
      721 tcgagcggtg agcgaacagg caatcgccgt tcgacagcat gaaattgaag ggcccgtgag
      781 gcctgacccg cgccgcgaat tcttccaagg cttcacgcag caccaggcga tcggggggag
      841 cttctggaaa gcgcaggcga agcgtttgca gcaggtggca gaacgtaagc tcgctgtcgg
      901 tctgcccaac cggcaggaag ctgccatcga gcgccggcgc gtaatcctcg agattgccgt
      961 tgtgcgcaaa gatccagtag cggccccaga gttcgcgcat gaaggggtgc gtattttcga
     1021 ggccgaccgc accctgggtg gccttgcgga tatgggcaat gacgttgagc gagtgaatcg
     1081 ggaaatggcg taccagctcg gccaccggtg attcgcatga gggctgcggg tcgaggaaga
     1141 cgcgcacgcc gcggccctcg aaaaaagcga tgccccatcc gtcgcgatgc acatcggtca
     1201 ctccgccacg cgcctgaaaa ccggtgaacg aaaaacagat gtcggtaggg acattgcaat
     1261 tcatccccag gagttggcac atggacgcag atccgtttgg tgtctgagta ttatcggaca
     1321 cactggtgaa aattgctaag ctgttctatc cgggaaacgc ctgccaggcc gggagatgca
     1381 cgatcaatac cccggatttg cgtttctctg acgctagcag cctgtcggac ttgaccaagt
     1441 cggctgcaaa aagtgggaag acggctcatt ttcccccgta tttctgtgcg aatagaacaa
     1501 ctattcgctt gaaatccgtg aaaaactg
//
LOCUS       DAOSIT010000601         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71646_length_1525_cov_7.854422, whole genome shotgun sequence.
ACCESSION   DAOSIT010000601 DAOSIT010000000
VERSION     DAOSIT010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71646_length_1525_cov_7.854422"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1525)
                     /locus_tag="PLL14_11420"
     CDS             complement(<1..>1525)
                     /locus_tag="PLL14_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOG04583.1"
                     /translation="RGDKPAMREKDLGIWQSWTWRDVAEEVRAIACGLAEMGFRRGEN
                     LAIVGENRPRMYMMMTAAQCLGGVPLPLYQDAVASEMLFVLLDAEIRFIFVEDQEQVD
                     KVLEIQSELPQLEHLFYDDPRGMRHYTLPFIHDIRELMAMGRIHNRNHPDLLASEVAA
                     GAADDVSVMLYTSGTTGRPKGVRLTHHAFISAARGAIDFDGLTADEDILSYLPMAWVG
                     DHLFSFAQAMVAGFTINCPESAETVMTDLREIGPTYYFAPPRVFENLLTQVMIRMEDA
                     SVVKQKMFHFFMAVARRCGAEILDGQPVSPGDRLLYALGKLFVFGPLKNVLGMSRIRV
                     AYTAGAAIGPDLFRFYRSIGINLKQLYGQTETCAYVCLQPDGQIKFDSVGKPAPGVEV
                     KIADNGEILVKGPMLLKEYYKRPDATAEAIDADGYFMTGDAGLFDDDGHLKIIDRAKD
                     VGKLANGAMYAPNYIENKLKFFQYIKEAVVFGDRREMVCAFINIDIGAVGNWAERRGI
                     "
BASE COUNT          293 a          475 c          468 g          289 t
ORIGIN      
        1 caatgccacg ccgctcggcc cagttgccga cggcgccgat gtcgatgttg atgaaggcgc
       61 agaccatctc gcggcggtca ccgaagacca ccgcttcctt gatgtactgg aagaacttca
      121 gcttgttctc gatatagttc ggcgcgtaca tcgccccatt ggccagtttg ccgacatcct
      181 tggcgcggtc gatgatcttc agatggccgt catcgtcgaa caatccggca tcgccggtca
      241 tgaagtaccc gtcagcgtcg atcgcttcgg cggtggcatc gggccgcttg tagtactcct
      301 tgagcagcat cggtcccttg acgaggattt cgccgttgtc ggcgatcttc acctcgacgc
      361 cgggcgccgg cttgccgacg ctgtcgaact tgatctgtcc atcgggctgc aggcaaacgt
      421 aggcgcaggt ttcggtctgg ccgtagagct gcttcagatt gatgccgatc gagcgataga
      481 agcggaacag gtccggaccg atcgccgcgc ctgcggtata ggcgacgcgg atacggctca
      541 tccccagcac gttcttcaac ggaccgaaaa caaagagctt gcccagggca tagagcaggc
      601 gatcgccggg cgaaaccggt tgaccatcga gaatctcggc gccgcagcgc cgggcaaccg
      661 ccatgaagaa gtggaacatc ttctgcttga cgacactggc gtcctccatg cggatcatca
      721 cctgcgtcag caggttctcg aagacgcggg gcggcgcgaa gtagtaggtc gggccgatct
      781 cgcgcagatc ggtcatcacc gtctcggccg actccgggca gttgatcgta aacccggcaa
      841 ccatcgcctg cgcaaatgaa aacaggtgat cgccgaccca ggccatcggc agataggaca
      901 ggatgtcttc atcggcggtc aggccatcga aatcgatagc accgcgagcc gcgctgatga
      961 aggcgtggtg cgtcagccgc acacccttcg gcctgccggt ggttccagag gtgtagagca
     1021 tcacgctgac atcatcggcg gcgcccgccg cgacctcgct ggccaggaga tcgggatggt
     1081 tccggttatg gatccgtccc atggccatca attcgcggat gtcgtgaatg aacggcaggg
     1141 tgtaatggcg catgccgcgc gggtcgtcgt agaacagatg ctcgagctgc ggcaattcgg
     1201 actggatctc gagcaccttg tcgacctgtt cctggtcttc gacaaagatg aagcggattt
     1261 ccgcgtcgag caggacaaac aacatttcac tggccacggc gtcctggtaa agaggcaagg
     1321 gcacgccccc gaggcactgc gcggcggtca tcatcatgta catgcgcggc cggttctcgc
     1381 cgacgatggc caggttctcg ccccgtctga agcccatttc ggccagtcca caggcgatcg
     1441 cacgcacctc ctcggcgaca tcgcgccagg tccaggactg ccagatgccc aggtccttct
     1501 cgcgcatcgc cggtttgtcg ccgcg
//
LOCUS       DAOSIT010000602         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71724_length_1524_cov_8.628319, whole genome shotgun sequence.
ACCESSION   DAOSIT010000602 DAOSIT010000000
VERSION     DAOSIT010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71724_length_1524_cov_8.628319"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1524)
                     /locus_tag="PLL14_11425"
     CDS             complement(<1..>1524)
                     /locus_tag="PLL14_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOG04584.1"
                     /translation="DSAVLLLVVAAIAVTVFRHFGLGSILGLLVTGVVVGPHTPGPFV
                     TTEVEGVRHFTELGVVLLMFLIGLEMKPRRLWELRRTLFGLGSLQIILSALVISAYFR
                     LFQDNWSTALLIGSSFALSSTAFVVQMLKDQGEMASGHGQTAFAVLLMQDLAVVPLLA
                     LVPILADAGPLPAGLPLWEQLTGAVAMVALVLLGGRYLLPRVLDHLARSNNREAFLLT
                     TLAAVFIAASAMEHAGMSMALGAFLIGMMLSTSRYSIQIEASMEPHKGLLMSLFFVAV
                     GMSVDVGTLAQHPLLFVAHVVSILAIKVAVLYGLCRCFGSARGTAFKVACMLSQGGEF
                     GFVLFGAAKALAVIDDLVFAMGVAIISVTMLLTPLLAKLGNHLAQRATDATAEGHEHL
                     LYPERDSESGARVVIGGYGRVGHTVGTILANSGISFIAFDTDAALVARWRSEGHPVFY
                     GDVGDPELLATGPLQSIELLVLTIDDENAAVRATVMVRAHAPQLGIVARARDLASCDA
                     "
BASE COUNT          328 a          496 c          478 g          222 t
ORIGIN      
        1 gcgtcgcagc ttgccaggtc acgcgcgcgg gccacgatgc cgagctgcgg agcatgcgcg
       61 cggaccatca ccgtggctcg aacggcggcg ttctcgtcgt cgatggtcag gaccaacagt
      121 tcgatgctct ggagcggtcc ggtggcgagc agttccgggt caccgacatc gccgtagaac
      181 accggatgac cctcgctgcg ccatctggcg accagggcgg cgtcggtgtc gaaggcgatg
      241 aaggagatac cgctattggc caggatggtg ccgaccgtat ggccgacgcg gccgtaaccg
      301 ccgatcacca cgcgcgctcc cgattctgaa tcccgctctg ggtaaagcaa atgttcgtgg
      361 ccctctgcgg tagcgtcggt cgctcgctgt gccaggtgat tgccgagctt cgccagcagc
      421 ggggtgagca gcatggtcac tgaaatgatc gccacgccca tcgcgaacac cagatcgtcg
      481 atgactgcca gcgccttggc agccccgaac agcacgaagc cgaactcgcc accctgcgag
      541 agcatgcagg cgaccttgaa cgccgtgccg cgggcgctgc cgaagcaccg gcacagcccg
      601 tacagcacgg ccaccttgat ggccaggatc gatacgacat gcgccacgaa caggagcggg
      661 tgctgcgcga gggtgccgac gtcgaccgac atgcccacgg cgacgaaaaa caggctcatc
      721 agcagccctt tgtgaggttc catgctggcc tcgatctgga tactgtagcg cgatgtcgaa
      781 agcatcatcc cgatcaggaa cgctcccagt gccatggaca tgccggcatg ttccatggca
      841 ctggcggcaa tgaataccgc cgccagggtg gtcagcagga aagcctcgcg gttgttgctg
      901 cgtgccagat gatcgagaac ccgcggcagc aggtagcggc caccgagcag caccagggca
      961 accatggcca ccgccccggt cagttgctcc cacagcggca ggccggccgg cagcggcccg
     1021 gcgtccgcga ggatcggcac cagcgccaat agcgggacta cggcaaggtc ctgcatcaac
     1081 aacacggcga aagcggtctg gccgtgaccg ctggccatct cgccctgatc cttgagcatc
     1141 tgcaccacga atgccgtgga ggacaacgca aaacttgagc cgatgagcaa ggccgtggac
     1201 cagttgtcct gaaaaagccg gaagtaagcg ctgatcacca aagctgacag aatgatttgc
     1261 agcgaaccca gcccgaaaag cgtacgccgt aactcccaca agcggcgtgg cttcatctcc
     1321 aggccaatca ggaacatcag caataccacc ccgagttcgg tgaaatggcg tacgccctcg
     1381 acttcggtgg tcacgaaggg accgggcgtg tgcggcccga cgactacgcc ggtgaccagc
     1441 agcccgagga tcgagcccag gccgaaatgc cggaacacgg tcaccgcaat cgcggcaacg
     1501 accaacagca acactgcgga gtca
//
LOCUS       DAOSIT010000603         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71802_length_1523_cov_9.166894, whole genome shotgun sequence.
ACCESSION   DAOSIT010000603 DAOSIT010000000
VERSION     DAOSIT010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71802_length_1523_cov_9.166894"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..515)
                     /locus_tag="PLL14_11430"
     CDS             complement(6..515)
                     /locus_tag="PLL14_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOG04585.1"
                     /translation="MSDDHQAALEALAAEVGQLLVRNGEKLATAESCTGGWVGQCLTA
                     IDGSSCWFERGFITYSNAAKIEMLGVDADALAAHGAVSEATAAAMALGAVQHSHADWA
                     LAITGIAGPGGGSPDRPVGTVCFAWAGPQRRIDTDTRHFQGDREAVRAQSVAHALTGI
                     LQRARHGLA"
     gene            complement(512..1045)
                     /locus_tag="PLL14_11435"
     CDS             complement(512..1045)
                     /locus_tag="PLL14_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOG04586.1"
                     /translation="MRTDKRWRLQKEDSITLASPPSIQFLLAHPAHLVACGFGSGLSP
                     RAPGTVGTLFAWASFPLLRPWMSDLEFLGLLFLCFVGGVLAVHKTGADLGVVDHGSIV
                     WDEIVPFWAVLLFCPTPGLWQATAFGLFRFFDIVKPQPARYFDEEVKNGFGVMCDDLV
                     AAGYTLLVLAILRFVLE"
     gene            complement(990..>1523)
                     /locus_tag="PLL14_11440"
     CDS             complement(990..>1523)
                     /locus_tag="PLL14_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOG04587.1"
                     /translation="NHAVPGDELWVSGQPGLAALGLAQLQGRTQLPAELARRCLDALQ
                     RPMARVELGLELRQRGLAHAAIDVSDGLLADLDHILERSGVAAEVFVAQLPPLPTGVD
                     PLLARRCQLAGGDDYELVFSAPPDKRAEITELAAQLALPIWRFGHVVSTGGSRLRLLD
                     ADGQALAFTERGFDHFG"
BASE COUNT          293 a          502 c          496 g          232 t
ORIGIN      
        1 ggccttcagg cgagtccgtg acgtgctcgt tgcaggatcc ccgtcagtgc atgcgccacc
       61 gactgtgccc gtaccgcttc ccggtcgcct tgaaaatggc gcgtgtcggt gtcgatgcgc
      121 cgttgcggcc cggcccaggc gaaacagacg gttcccaccg gccggtcagg tgagccgccg
      181 cccgggccgg ctatgccggt gatggccagt gcccagtcgg catggctgtg ctgcaccgct
      241 cccaaggcca tggccgcggc ggtcgcttcg ctgaccgcgc catgcgctgc cagggcgtcg
      301 gcatcgacac ccagcatctc gatcttggcg gcattcgaat aggtgatgaa gccgcgctcg
      361 aaccagcatg aactgccgtc gatcgcggtc aggcactgtc ccacccagcc gcccgtacag
      421 gattcggccg tggccagctt ctcgccgttg cgcaccagca gttgcccgac ttcggccgca
      481 agcgcttcga gcgctgcctg atggtcgtca ctcattcgag cacgaagcgc agaattgcca
      541 ggaccagcag cgtataaccg gcagcgacga gatcatcaca catcacgccg aagccattct
      601 tcacctcttc gtcgaagtag cgcgctggct gcggcttgac gatgtcgaaa aagcggaaca
      661 ggccgaaggc ggtggcttgc cataagcccg gcgtcgggca gaacagcagc accgcccaaa
      721 aggggacgat ttcgtcccag acgatgctgc catggtcgac gaccccgagg tctgcgccgg
      781 tcttgtgcac tgccagaacc ccgccgacga aacagaggaa gagtaaaccg aggaactcca
      841 ggtcgctcat ccacggccgc agcagcggga aggacgccca agcgaagagt gtgcccacgg
      901 tccccggcgc ccgtggcgag agaccgctac cgaagccgca ggccaccagg tgcgccggat
      961 gcgcaagcag aaactggatt gaaggggggc tagccaaagt gatcgaatcc tctttctgta
     1021 aacgccagcg cttgtccgtc cgcatcgagt agccgcagac gagaaccgcc ggtactgacg
     1081 acgtgaccga aacgccagat gggcaaggcg agttgcgcag cgagttcggt gatttcggcg
     1141 cgcttgtcgg gtggcgcaga gaacaccagt tcgtagtcgt cgccgccggc gagctgacac
     1201 cgacgagcca gcagtgggtc aactcccgtc ggcagtggcg gcaactgcgc cacgaagacc
     1261 tctgcggcaa cgccggagcg ctcgaggata tgatcgagat cggcgagtag cccatccgaa
     1321 acgtcgatcg cggcatgcgc caggccacgc tggcgcaatt ccagcccgag ctcgacgcgc
     1381 gccatggggc gttgcaaggc gtcaagacag cgtcgtgcca actccgcggg cagttgcgtc
     1441 cggccctgca gttgggccag ccccagagcg gcaaggccgg gttgtcctga aacccagagc
     1501 tcgtcgccgg gcacggcgtg gtt
//
LOCUS       DAOSIT010000604         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_71811_length_1523_cov_4.000681, whole genome shotgun sequence.
ACCESSION   DAOSIT010000604 DAOSIT010000000
VERSION     DAOSIT010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71811_length_1523_cov_4.000681"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PLL14_11445"
     CDS             complement(<1..110)
                     /locus_tag="PLL14_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOG04588.1"
                     /translation="MHIDWSSFTPWQAVSGGILIGIGSVVLVLWNGRIAG"
     gene            525..1409
                     /locus_tag="PLL14_11450"
     CDS             525..1409
                     /locus_tag="PLL14_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG04589.1"
                     /translation="MSLSELIHQFNSQPQGVPGSRFASRFRLSGDGRVFVHFANLVLE
                     TVFLPITAVASGSTIGHAAALRVLGLSTGNPVSADAVFVLPADDDEFVWVDRLVRTLH
                     ALSYLAHPVHGTLLLKVNRRHVMSVPSDHGLSFEEILHACGLRPQHITLEIDADGIED
                     VDHFSRAIAAYRRRGYLISLSGFGRTSVDLSLLGTLHPDIVKFDRLLLASARSLEYLV
                     DRIHDLGASVAIECPGDAHWQPFAGATRADLIQHFQPVDRSGATSPRQSTGAKATPFD
                     QANVRPTVSGLPSRRRPE"
BASE COUNT          297 a          491 c          426 g          309 t
ORIGIN      
        1 atgccggcaa tacggccgtt ccacagaacc aacacgacgc tgccgatgcc aatcaggatg
       61 ccgccagaca ccgcctgcca gggggtaaag ctgctccagt ctatgtgcat gagaaactcc
      121 gaatgggtgc aaccgcagct gatcgagggc tactgcctca ggcggctact gggacgcggt
      181 cggcatcgct tcagtgctgt ggagacaggt ctatcaacag cactccacaa ggtctgtgtt
      241 cagatccaca ccatgcgaaa aaataacgcg aaccctgcac agatcgccgc gaatggagag
      301 cttagttggc gtgagagctg cggcaaacta agatttttct cttaacttat ctccgccacc
      361 ggataagcaa atggcaagtt agtgtttgcc agaagtgacc ctgccgcctt aaattcaggc
      421 atccgctgtc gcctggttcc gaaaccagat gcgagtgtat ctcgccaggc ggcacggtcc
      481 gaacgcaaga agtgcttgct ggatccactt cgggagtaaa cccaatgtcg ctgtcggaac
      541 tcattcatca attcaacagc cagccccagg gggtgccggg cagccgcttt gcctcccggt
      601 ttcgtctctc cggagatggg cgcgtgttcg tgcatttcgc caacctggtc ctcgaaaccg
      661 ttttcctgcc catcaccgcc gtcgcttccg gcagtaccat cggtcatgcg gccgcgctac
      721 gggtcctcgg cctgagcacc ggcaacccgg tcagcgcgga tgccgttttc gtcctgcctg
      781 ccgacgatga tgagtttgtc tgggtggacc gtctggtccg gacactgcac gctctcagct
      841 acctggccca tccggtacac ggaacactgc tgctcaaggt caatcggcgg cacgtcatga
      901 gcgtcccaag cgaccacggg ctgtctttcg aggaaattct gcacgcttgc ggcctccgcc
      961 ctcaacacat caccctcgag atcgatgcgg acggaattga ggacgtcgat catttttcgc
     1021 gggccatcgc tgcttatcgc cgacgtgggt acctgatctc cctgagcggc tttggacgaa
     1081 cctcggtcga tctttcgctg ctgggcacgc tgcaccctga catcgtcaag ttcgaccgac
     1141 tgcttctcgc ttcggcgcga tcccttgagt atctggtcga ccggattcac gacctgggcg
     1201 ccagcgttgc catagagtgc cccggtgatg cgcactggca accattcgcc ggagcgaccc
     1261 gcgccgacct gatccagcac ttccagcccg tcgaccggtc gggagcgact tcgccacgcc
     1321 agtctacggg tgcgaaagcg acgcctttcg accaagcgaa tgtcagacca acagtttctg
     1381 gtctgccgag ccgacgcaga ccggagtgac gtgcaactgc atcaccaggc atacttcagt
     1441 ggtctgaccg cagcgatgct gcgctgctcc tccgtgtgct gctgcgaccg ccggcagtcc
     1501 ggcaatccct tcacgaaacg gcg
//
LOCUS       DAOSIT010000605         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72018_length_1520_cov_7.926962, whole genome shotgun sequence.
ACCESSION   DAOSIT010000605 DAOSIT010000000
VERSION     DAOSIT010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72018_length_1520_cov_7.926962"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLL14_11455"
     CDS             complement(<1..398)
                     /locus_tag="PLL14_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOG04590.1"
                     /translation="MNIRRLLLIAALAGLVLAYFALDLGRFLSLDYFKSQQLTIEAWR
                     AAQPLQAALLFFATYVAVTGLSLPGATVMTLVAGAVFGLFWGLLLVSFASSLGATLAF
                     LVSRFLLRDWVQQRFGDRLRAINAGVEKEGG"
     gene            complement(477..1475)
                     /gene="arsS"
                     /locus_tag="PLL14_11460"
     CDS             complement(477..1475)
                     /gene="arsS"
                     /locus_tag="PLL14_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767285.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HOG04591.1"
                     /translation="MHATLPLLLTSNFPNLARRSVETLQVNLGYRCNQSCLHCHVNAG
                     PQRQEEMTAETIDALLAFIAASPEVKALDLTGGAPELNANFRRLVVAGRERGLRVIDR
                     CNLTILEEVGHEDLAAFLAAHRVEIVASLPCYLEENVDRQRGKGTFDASIRGLRQLNA
                     LGYGVPGGPLLLNLVFNPQGPTLPPPQGPLTAAYREHLGRNFGIVFNQLFTLTNMPIQ
                     RFGSLLVSKGQFNAYMQLLHSAHRDENVEHVMCRNLLSIDWQGYLYDCDFNQQLGLPI
                     RGAERARLHITDVQAAALDGWKIRVADHCYGCTAGQGSSCGGALGEEVAAAPTCSA"
BASE COUNT          303 a          463 c          493 g          261 t
ORIGIN      
        1 cccccttcct tctcgacgcc ggcattgatc gcccgcaggc gatcgccgaa gcgctgctga
       61 acccagtcgc ggagcaggaa acgcgagacc aggaaggcga gggtggcacc cagcgacgaa
      121 gcgaaggaaa ccagcagcaa gccccagaac aggccgaata cggcgcccgc gaccagggtc
      181 atcacggttg cgccaggcag cgaaagaccg gtcaccgcca cataagtggc gaagaacagc
      241 agagccgcct gaagcggctg cgcggcccgc caggcctcga tcgtgagttg ctggctcttg
      301 aagtagtcga gactcaggaa gcgacccagg tcgagagcaa aatacgccag taccaacccg
      361 gccagggcag caatcagcag cagtcttcga atattcatgg cagctttccc acctcttgat
      421 gatcggtaca gcgctatgtc gtgcggcagt ttgcagacct tacaatgcga gccggatcag
      481 gcgctgcagg tcggtgccgc cgcgacttcc tcacccagcg ccccaccgca actcgacccc
      541 tggcccgccg tacagccgta gcagtggtcg gcgacccgga tcttccagcc gtcaagcgcc
      601 gcagcctgca cgtcggtgat atgcaggcgg gcgcgttcgg cgcccctgat cggcaagccg
      661 agttgctgat tgaagtcgca gtcgtagagg tagccttgcc agtcgatcga cagcagattg
      721 cggcacatca cgtgctcgac attctcgtcg cggtgggcgc tgtgcagcaa ctgcatatag
      781 gcgttgaact gtcccttcga gaccagcagc gacccgaaac gctggatcgg catgttggtc
      841 agcgtgaaca actggttgaa aacgataccg aagttgcgcc cgaggtgctc gcgatacgcc
      901 gctgtcagag gtccctgcgg tggcggcaac gtcggcccct gtggattgaa gaccaggttc
      961 aggagcaggg ggccgccggg cactccgtac cccagtgcat tcagttggcg caggccgcgg
     1021 atgctggcgt cgaaggtacc cttgccgcgc tgccggtcga cgttctcttc gaggtagcag
     1081 ggtagcgatg cgacgatttc gacacgatgc gcagcgagga aagcggcaag atcttcgtgg
     1141 ccgacttcct cgaggatcgt cagattgcaa cggtcgatga cccgcagacc gcgttcgcgc
     1201 ccagcgacga ccagtcgccg gaaattcgcg ttcaactcgg gcgccccgcc agtgaggtcc
     1261 agcgccttga cttcggggct ggcggcgatg aaggccagta acgcgtcgat cgtctccgca
     1321 gtcatttcct cctggcgctg tggaccggca ttgacatggc agtgcaggca actctgattg
     1381 cagcgataac cgaggttgac ctggagggtt tcgaccgaac gccgggccag gtttggaaaa
     1441 ttgctggtca gcagcagggg cagagtggcg tgcatggtgg gttctctcgc gggttgttct
     1501 ggtgacctgt gtcgaaggga
//
LOCUS       DAOSIT010000606         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72168_length_1518_cov_11.135338, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000606 DAOSIT010000000
VERSION     DAOSIT010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72168_length_1518_cov_11.135338"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLL14_11465"
     CDS             complement(<1..734)
                     /locus_tag="PLL14_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOG04592.1"
                     /translation="MSLTLPAGMQISAPIQAGYETILTPAALALVARLHRAFEGRRQE
                     LLQARIVRQARIDAGEMPDFLPETRHIREGDWKISPLPKALERRRTEITGPVEAKMII
                     NAYNSGADSYMSDFEDSNSPKWDNQIQGQVNLYKAIRRQLSFINEAGKEYTLNDKIAT
                     LQIRPRGWHLDEKHVTVDGQRVSGGIFDFALVFFHNAHEQIARGAGPFYYLPKMESHL
                     EARLWNDIFIMAQEHIDLPRGTIKATV"
     gene            851..>1518
                     /locus_tag="PLL14_11470"
     CDS             851..>1518
                     /locus_tag="PLL14_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG04593.1"
                     /translation="MDHIKQIEAFVNSATRGSLSAAAQLEGITPAVIGRRLDALEARL
                     GVKLLLRSTRKLSLTFEGQAFLEDCQRILNDLANAEAAVSLGGIRASGHLKVSAPAGF
                     GRRHVAPQVAAFMLANPDITVRLDLTDRLVDLLNEGVDCAVRIGEMADSSLASNKLGE
                     MHRVVVASPAYLAAHPTPRTPAELATHNCLSLGQQRGWALRQTPGGEVANIKVSGTFE
                     CNDGA"
BASE COUNT          272 a          425 c          491 g          330 t
ORIGIN      
        1 accgtggcct tgatcgttcc ccgcggcaga tcgatgtgct cctgggccat gatgaagatg
       61 tcgttccaca ggcgcgcttc gagatgcgac tccatcttcg gcaggtagta gaaggggcct
      121 gcgccgcgcg cgatctgttc gtgggcgttg tggaagaaga ccagcgcgaa gtcgaagatg
      181 ccgccggaaa cccgttgtcc gtcaacggtg acgtgctttt cgtccagatg ccaaccgcgc
      241 ggccggattt gcagcgtggc gatcttgtcg ttgagggtgt attccttacc ggcctcgttg
      301 atgaaggaaa gctggcggcg gatcgccttg tagaggttga cctggccctg gatctggttg
      361 tcccacttcg ggctgttgga gtcctcgaag tcgctcatgt acgaatcggc gcccgagttg
      421 taggcgttga tgatcatttt cgcttcgacg ggtccggtga tttcggtacg gcggcgctca
      481 agagcttttg gcagcgggga aatcttccag tcgccttcgc ggatgtgccg cgtttccggc
      541 aagaagtcgg gcatctctcc ggcgtcgatg cgcgcctggc ggactatccg tgcttgcagc
      601 aattcctgac ggcgaccttc gaaagcgcgg tgcagcctgg cgaccagagc cagcgctgcg
      661 ggagtgagga tggtttcgta accggcttgg atcggcgcgc tgatctgcat tccggcgggc
      721 agcgtgaggc tcatgtggtg gctcctggct atgggttatg gtgcggtgca aggatcttat
      781 taaatgcctt agtggaaaat aagatcgatt agaatcaatt gattttttac ttcaggtatc
      841 gaataagcca gtggatcata tcaagcaaat cgaagcgttc gtcaactcgg caacacgtgg
      901 cagtctttcg gctgcggcac agcttgaagg gattaccccg gcggtgatcg ggcgccgcct
      961 ggatgccctt gaggcacgcc tgggcgtcaa gctactgctg cgctcgacgc gcaaactgtc
     1021 gctgacattc gaagggcagg cttttctcga ggactgccaa cggattctca acgacctggc
     1081 caacgccgaa gcggcggttt cgctcggcgg cattcgcgcc agtggtcatc tcaaggtgtc
     1141 tgcccccgca ggtttcgggc gccgacatgt ggcgccgcaa gtggcggcct tcatgctcgc
     1201 caatcccgat atcacggtac ggctcgatct gacagatcgt ctggtcgatc tgctcaacga
     1261 gggtgtggac tgtgccgtac gcatcgggga aatggccgat tcgagcctgg cgagcaacaa
     1321 gctcggcgag atgcaccggg tcgtggtcgc cagtcccgct taccttgcag cgcaccccac
     1381 cccacgtacg ccggccgaac tggcgaccca caactgcctg tcccttggcc agcaacgtgg
     1441 ctgggcgcta cgtcagacgc cgggtggaga agtggcgaat atcaaggttt cggggacttt
     1501 cgagtgcaac gacggcgc
//
LOCUS       DAOSIT010000607         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72528_length_1513_cov_8.749657, whole genome shotgun sequence.
ACCESSION   DAOSIT010000607 DAOSIT010000000
VERSION     DAOSIT010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72528_length_1513_cov_8.749657"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..619)
                     /locus_tag="PLL14_11475"
     CDS             complement(35..619)
                     /locus_tag="PLL14_11475"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HOG04594.1"
                     /translation="MRAPDNVSFMPWLRDRGSLTARIQARGHFAVRVLRQGLDSPTAD
                     EALALGLPRQARAWVREVALSCDGQIVVFAHTVLPHRPRGPLSLWLARLGNRSLGALL
                     FAHPGFERGTMKFKRLDRRQALFTPALAALQLADGATSALWARRSQFDFAGQSVLVTE
                     VFSPRVLRLSPSGKENSIFQFRYEPERATEWVTR"
     gene            complement(686..1129)
                     /locus_tag="PLL14_11480"
     CDS             complement(686..1129)
                     /locus_tag="PLL14_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG04595.1"
                     /translation="MPRSAFSFFPLALLLVALPVAAQMPRIELTAGFYRIEAEVAAND
                     PNRMQGLMHRRSMPANQGMLFVFAQPERHCMWMRNTLLPLSVAFLDEEGRILNIENMQ
                     PQTEDNHCAAKAARFALEMNLGWFASKGIKPGSRIGGLEKSPAPQ"
BASE COUNT          267 a          472 c          494 g          280 t
ORIGIN      
        1 cgttagactt cccttttcaa cccctcaacc cacatcaccg cgtcacccac tccgttgctc
       61 gttcaggctc atacctgaat tggaaaatac tgttttcttt tccggatggt gagagtcgca
      121 gcacgcgtgg tgaaaagacc tcggtcacca ggacggattg cccggcgaaa tcgaactgcg
      181 agcggcgtgc ccagagcgcg ctcgtagccc catcggcaag ttgcagggcc gccaacgccg
      241 gtgtgaacaa cgcctgccgg cgatctaggc gcttgaactt catcgtgccg cgctcgaaac
      301 cgggatgcgc gaagagcaag gcgcccagcg agcggttgcc gaggcgagcc agccagaggc
      361 tgagcggacc tcgcggtcga tggggcagca cggtatgcgc gaagacgacg atctgaccat
      421 cgcaggacaa ggccacttcc cgcacccagg cccgcgcctg gcgtggcaac ccgagcgcca
      481 gtgcttcatc ggccgttggg ctgtcgaggc cctggcgcag cacccgaacg gcaaaatgtc
      541 ctcgcgcctg gatgcgagcg gtcagcgagc cgcgatcgcg caaccatggc atgaaggaca
      601 cgttgtcggg cgcgcgcatc aagctgctgc gccaggaccg accggcggtg acgctcactt
      661 gccgcagacg gcgtactccc gccgcttatt gtggcgcagg ggatttttcg agaccgccaa
      721 ttcgactccc tggcttgatg cctttgctcg caaaccagcc gagattcatt tcaagcgcaa
      781 agcgtgccgc cttcgctgca cagtgattgt cctcggtctg cggctgcatg ttctcgatgt
      841 tcagaatgcg cccctcttcg tcgagaaagg caaccgacag gggtagcaag gtgttgcgca
      901 tccacatgca atggcgttcg ggctgcgcga agacgaagag catcccctgg ttggccggca
      961 tgctgcggcg atgcatcaat ccctgcatcc ggttcgggtc gttggccgcc acctcggcct
     1021 caatgcggta gaagccggcc gtcagttcga tgcgcggcat ttgcgccgca accggcagtg
     1081 caaccagcag cagggcaagg gggaaaaagg aaaaagcgga cctgggcatg caagactccg
     1141 gcgtagacga ctatcggggg tcggaaaggt gatcggtatg gatggcgaga gagtcgtacg
     1201 catcacgtgt cgcacgtccg cagcaggcac ggccctgtga tcacggttac gatttcttcc
     1261 gtggacctcc cggcgcggcc ttcgcgggag ccttccggtt tgttggcgcc ttggcagcag
     1321 gggccgtgtc cgggcgtgat cccttggtgg cggttgctgg cttggccgtc gtcactggtt
     1381 tgccgggatg ggccagcggc ttcttcgctg cgccggctac aggcggtttg ggtgccttga
     1441 ccaccgcttg cggggcagtt cgtgctgccg gtttcggcgg ggccgcggct tcaccgaagg
     1501 tcaccgccgc atc
//
LOCUS       DAOSIT010000608         1510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72750_length_1510_cov_8.529210, whole genome shotgun sequence.
ACCESSION   DAOSIT010000608 DAOSIT010000000
VERSION     DAOSIT010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72750_length_1510_cov_8.529210"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..>1510)
                     /locus_tag="PLL14_11485"
     CDS             complement(100..>1510)
                     /locus_tag="PLL14_11485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG04596.1"
                     /translation="AQIMTDATSEGLFAAGVAEILAKHIVDALPIPMFAVDNEHRVIL
                     WNVPCERLTGIPARFMIGRTGAWSAFYSSERPVMADLVLTGCRVEDLERHYAGKYHPS
                     ALIENGWEFEDFFPQLPAGGKWLAFTASPLRDEIGRIVGAVETFRDITAQREAERTAR
                     DVAVLLSEIVQGCPVPMFVIDAEHRVTHWNRACESMSGVPSEQMIGSRQHWWPFYPEP
                     RPVMADLVLDAAAGEVAKFYAGKYQPSPLMAGAWEAKDHFPNFPGGGRWLYFTAAPLH
                     GADGRVIGAVETLQDISEQKRYEAQLESQARQDLLTGLANRRVLEERLKQALSQAARD
                     DRTVAVAFIDLDRFKPINDELGHAAGDTLLREVASRLTATVREVDTVARIGGDEFVVM
                     LSAPESVEAVEGVVRRIIAEVSRPFEVAGQVVQVGCSVGIALYPEHGPDQTSLIGHAD
                     TAMYIAKASGRNTYHIHAP"
BASE COUNT          287 a          504 c          447 g          272 t
ORIGIN      
        1 aaccctgcaa tggttgatcg gcagttgcgc tgagactttc tgccgccgcc caaggttcag
       61 gcaggggtgg catccggctg tcgattgcgc gcacactgcc tacggcgcgt ggatgtggta
      121 ggtattgcgc ccggaagcct tggcaatgta catcgcggta tcggcatggc caatcaggct
      181 ggtctgatcc gggccatgct ccgggtacag cgcaatcccg acgctgcagc ccacctgcac
      241 cacctgtccg gcgacttcga acggacggct gacctcggcg atgatgcgac gaaccacgcc
      301 ctcgacggct tccacagact ctggcgcgga caacatgact acaaactcgt cgccaccgat
      361 ccgcgcaacg gtatcgacct cgcgcacggt cgccgtaagg cggctggcga cttcacgcaa
      421 cagggtgtct cctgccgcat gcccgagttc gtcattgatc ggcttgaagc ggtcaaggtc
      481 gatgaacgcg acggcgaccg tacggtcgtc gcgcgcagcc tgtgagagtg cctgtttcag
      541 acgctcttcg agcaccctgc gattggccag cccggtcagc aaatcctgcc gcgcctggct
      601 ttccaactgg gcttcgtatc ttttttgctc gctgatgtcc tgcagcgtct cgacggcgcc
      661 gatcacccgc ccatcggcgc cgtgcagcgg tgcggccgta aaatacaacc accgcccacc
      721 gcccgggaaa ttggggaagt gatccttggc ttcccaggcg cccgccatca agggggaagg
      781 ctggtacttt ccggcataga acttcgccac ctccccggcc gcggcatcca gaaccaggtc
      841 ggccatcacc ggccgtggct cgggataaaa tggccaccaa tgctgccggg agccaatcat
      901 ctgttccgac ggcacgccag acatcgactc gcaggcgcga ttccagtgcg tgacacgatg
      961 ctcggcgtca atgacgaaca tcggcaccgg gcagccttgc acgatttccg acaagagcac
     1021 ggccacgtca cgcgccgtcc gttctgcctc ccgctgcgcg gtgatgtcgc gaaaggtctc
     1081 gacggctccg acgatacggc caatctcgtc gcgcagcggc gacgcggtaa acgccaacca
     1141 cttgccgccg gccggtagct gcggaaagaa atcctcgaat tcccagccgt tctcaatcaa
     1201 cgccgaggga tgatacttgc ccgcgtagtg gcgttcgaga tcctcaacac ggcagccggt
     1261 caataccaga tcagccatta ccggccgctc gctcgaataa aacgccgacc atgcgccagt
     1321 tctgccgatc atgaagcggg cgggaatccc ggtcagccgc tcgcagggaa cattccagag
     1381 gatgacgcgg tgctcgttgt cgacggcgaa catcgggatg ggcaaggcat cgacgatgtg
     1441 cttcgccagt atttccgcta ccccagccgc aaagaggcct tcgctcgtcg cgtcggtcat
     1501 gatctgtgcc
//
LOCUS       DAOSIT010000609         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72823_length_1509_cov_15.327373, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000609 DAOSIT010000000
VERSION     DAOSIT010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72823_length_1509_cov_15.327373"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1509
                     /locus_tag="PLL14_11490"
     CDS             255..>1509
                     /locus_tag="PLL14_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOG04597.1"
                     /translation="MSHFIVTDRKTDYLLPPSLDDWLNQDHLARFIVEVVDQLDLSKL
                     TRQYAGRGSKAYHPATLLAILVYGYATGIFSSRRLERATYDSVAFRYIAAGSHPDHDS
                     LATFRRRFLDELSDLFLQVLAMAKEMKLLKLGNVCLDGTKIDANASRHSALSHGHIEK
                     LEAQLKAEVQELFSLAEQADQADVPDGVSLPEEIKRREDRLAAMAVAKAKIAARAEER
                     YQREKAEYDEKMARRAAKEEAAGKKLGGKPPKAPEPGARDSDQINLTDEESRIMPVAG
                     GRFEQAYNAQAGVDAATMLVVAVGVTQAPNDKEQVEPMLATLKAQAEVLGPVESLIAD
                     SGFCSEKNIKACEAASIDPVIAVARDEHHPDWRERHSEPAPLPENATPVQALSHRLKT
                     KAGRALYALRKQTVEPVFGIIKSVMG"
BASE COUNT          327 a          460 c          469 g          253 t
ORIGIN      
        1 tcgccgatgt tggcgcggaa ggcggttacc tgctggtcgg gccgatcgcc agaacgaagt
       61 agcacccctg gtccaccact cgcgtcataa cgtcgttgca cttcagagtt gaatctgcct
      121 cctcgataaa tcgcttcatt cctgcacctc cgtacaggaa ttaacgcacc acgccctagc
      181 agcctgtcgg actttcggct tgctccccac cggatatgct ataatcatgc cttttgaagc
      241 aaccgagggc cggcatgtcc cacttcatcg tcaccgaccg taagaccgac tacctgctac
      301 cgccgtcact ggacgattgg ctgaaccagg atcacttggc gcgattcatc gtggaggtag
      361 tcgaccagct ggacctgtcg aagctgacgc gacagtacgc tggacgtgga tcgaaggcgt
      421 accatccggc gaccctgctg gcaattctgg tctatggcta cgccacgggt attttctcca
      481 gccgcaggct ggagcgggca acctacgatt cggtagcctt ccgctacatt gccgccggaa
      541 gccatcccga tcacgacagc ctggcgacgt tccgccggcg ttttctggac gaactgagcg
      601 acctgtttct gcaggtcctg gcgatggcca aggaaatgaa gctgctcaag ctaggtaacg
      661 tctgtcttga cggcacgaag atcgacgcca acgcctcaag gcacagcgcg ctctcccacg
      721 gacacatcga aaagctggaa gcccagctca aggctgaggt gcaggaactc ttcagcttgg
      781 cggaacaggc ggatcaagcc gacgttccgg acggcgtcag cctgcccgaa gaaatcaagc
      841 gtcgtgaaga tcggctggcg gcgatggcag tggccaaggc gaagattgct gcccgggccg
      901 aggagcgcta tcagcgagag aaagccgagt acgacgagaa aatggcgcgg cgcgcggcca
      961 aggaagaagc ggccggcaag aagttgggcg gcaagccgcc gaaagccccc gagcccggcg
     1021 cacgggacag tgaccagatc aatctgaccg acgaagaatc gcgcatcatg ccggtggccg
     1081 gtggccgctt cgaacaagcg tacaacgccc aggccggggt tgatgcggcg acgatgctgg
     1141 ttgtggcggt cggcgtgacg caagccccca atgacaaaga gcaggtcgag ccgatgctgg
     1201 cgacgctcaa ggcacaagcc gaggtgctgg gtcccgtgga aagtttgatc gccgacagtg
     1261 gcttctgcag cgagaagaac atcaaggcat gcgaggccgc cagtatcgat ccggtgatcg
     1321 cggtggctcg cgacgagcac caccctgact ggcgggagcg gcacagcgaa ccggcgccgc
     1381 tgccggagaa tgcgacaccc gtgcaggccc tgtcgcatcg cttgaagacc aaagcgggac
     1441 gagccctcta tgccttgcgc aagcagaccg tcgagccggt cttcggcatc atcaaatcgg
     1501 tcatgggct
//
LOCUS       DAOSIT010000610         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_72912_length_1508_cov_8.246387, whole genome shotgun sequence.
ACCESSION   DAOSIT010000610 DAOSIT010000000
VERSION     DAOSIT010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72912_length_1508_cov_8.246387"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLL14_11495"
     CDS             <1..950
                     /locus_tag="PLL14_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG04598.1"
                     /translation="KIELENTVFAPAQLMRETQALFAGSARDKGLELRAYWRAAPAQR
                     YRSDAHRLRQMLSNLVGNAIKFTAQGGVRIEGGEVERADDAALLEFSVSDTGIGIPAE
                     KIDLLFKPFSQADSSTTREFGGTGLGLSIVNSLAKLIGGDVGVDSVPGQGSRFWFRVR
                     VEVVGAGVDSTVVVANRAEEAMVRPLRRGHVLVAEDNAINRLVIKAMLGKLGFQVSLK
                     ENGQEAVGAIVNDERFDLVLMDLSMPVLDGCGATEQIRRWEAENNRPRLPIIAFTAGA
                     FDEDRVRSLAAGMDDFLTKPVNRDALQGLLDKWLRPPPA"
     gene            complement(973..1332)
                     /locus_tag="PLL14_11500"
     CDS             complement(973..1332)
                     /locus_tag="PLL14_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG04599.1"
                     /translation="MNPLVEGWTRADAEAVLERGDVREIRYVPVSVGLEAPACGPEWA
                     ENICLALARHVDEYVRGNALLGLGHLARTCRAVSSRQAVVEAVAAGLCDQSRYVRGHA
                     LDAADDLQIFAGIVLPR"
     gene            complement(1385..>1508)
                     /locus_tag="PLL14_11505"
     CDS             complement(1385..>1508)
                     /locus_tag="PLL14_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012841747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOG04600.1"
                     /translation="FARVPCGGTHVRRTGEIGSIRLKRNNIGRGKERVEILLDA"
BASE COUNT          240 a          505 c          516 g          247 t
ORIGIN      
        1 gcaagatcga gctcgaaaac accgtcttcg cgcccgcgca actgatgcgg gaaacccagg
       61 ccctgtttgc cggctcggcc agggacaagg gtctggagtt gcgtgcgtac tggcgcgccg
      121 cgcccgcgca gcgttaccgt tccgacgccc accgcctgcg ccagatgctc agcaacctgg
      181 tcggcaacgc catcaagttc accgcgcagg gtggcgttcg catcgagggc ggcgaggtcg
      241 aacgtgcgga cgacgccgcg ctgctcgaat tctcggtcag cgataccggc atcggtattc
      301 ccgccgagaa gatcgatctg ctgttcaagc cgttctcgca ggccgacagc tcgacgacgc
      361 gcgaattcgg cggcaccggc ctgggcctct cgatcgtgaa cagcctggcc aaactgatcg
      421 gtggcgacgt tggcgtcgac agcgttcccg gccagggttc gcgcttctgg ttccgcgtgc
      481 gcgtcgaggt cgttggcgcc ggcgtcgata gcactgtcgt cgtcgccaat cgcgccgagg
      541 aggcgatggt gcgcccgcta cggcgcggtc acgtgctggt ggccgaggac aacgcgatca
      601 atcgtctggt catcaaggcg atgctgggca agctcggctt ccaggtcagt ctgaaggaga
      661 atggccagga ggcggtcggc gccatcgtca atgacgagcg cttcgacctg gtgttgatgg
      721 atctctcgat gccggtgctg gacggttgcg gcgcgaccga gcaaatccgg cgctgggagg
      781 cggaaaacaa ccggccgcgc ctgccgatca ttgccttcac cgccggcgcc ttcgacgaag
      841 accgggtgcg cagtctcgcg gcgggcatgg acgactttct caccaagccg gtcaatcggg
      901 atgcgctgca gggcttgctg gacaagtggc tgcgcccgcc gccggcctga aaccgcaggt
      961 gcgccgccgg cgtcagcgcg gcagcacgat gccggcgaag atctggagat cgtcggccgc
     1021 atccagcgca tgaccgcgaa cgtaccggct ctgatcgcac agcccggcgg cgacggcctc
     1081 gacgacggcc tgacggctgg agaccgcgcg gcaggtccga gcaaggtgcc cgagaccgag
     1141 cagggcgttg ccgcggacat attcgtcgac gtgccgcgcc agggccaggc agatgttctc
     1201 cgcccactcc ggcccgcagg ccggggcctc gaggccgacg ctgaccggga cataacggat
     1261 ctcgcgtaca tcgccgcgtt cgagcactgc ctcggcgtcg gctctcgtcc agccctccac
     1321 gagtggattc attcgtcgtg cccagtgggt gtcgcggatg gccagcgcgg gtcgaacttc
     1381 ccgctcatgc gtcgagcagg atttcgaccc gttccttgcc ccgcccgatg ttgttgcgct
     1441 tgagccggat gctgccgatc tcgccggtgc gccgcacgtg cgttccgcca cacggcacgc
     1501 gcgcaaag
//
LOCUS       DAOSIT010000611         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_73013_length_1506_cov_12.541006, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000611 DAOSIT010000000
VERSION     DAOSIT010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73013_length_1506_cov_12.541006"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLL14_11510"
     CDS             complement(<1..787)
                     /locus_tag="PLL14_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOG04601.1"
                     /translation="MTTASVSPPASRLLFLPWLILFAMLGATWFVWDHERQNARKELH
                     TQFDFALRDTVSRIEQRVAAYEQLLRGVQGLFATTGLRNRQAIHDYVETLRGDPNLSG
                     MQVIGVIERVPSAGKAAHVRAIRRLGPADYSIHPQGERETYAPVIQRESYSGRHQAPL
                     GLDTWANPVRRLAMETARDSGLAAITGKVQLAIDNGVEGNPGFIMYLPVFAQGQPRDN
                     VTQRRDSLIGWVYASFHVRDFMASLYGRQAEGLAFAIYDGAVPS"
     gene            complement(796..>1506)
                     /locus_tag="PLL14_11515"
     CDS             complement(796..>1506)
                     /locus_tag="PLL14_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG04602.1"
                     /translation="DCSRPFTLALHEHGLLYSADTDSGIDKDVADELIRRSGCKVTTS
                     LLARARIWQLIESGALDFTLSGISNAERDKFAGFAWYFSNKYYLLVRKDAGVRNQADF
                     EQNDKLQLGVIRGFRYSPSANRLVDKLHAASRVSQAGGLAPLYQTLLLGHIHGMIIEP
                     FDYPTLEEKRIRDVTSIIEFSDATVPHGLVMSKTSLPPAEQEKWRALINGMRSDGSMR
                     RIFEKYFKADLAAAMVDF"
BASE COUNT          273 a          454 c          503 g          276 t
ORIGIN      
        1 cactggggac ggcgccgtcg tagatggcga aagcgaggcc ctcggcttgc cggccgtaca
       61 gactggccat gaagtcgcgc acgtggaacg aggcatagac ccagccaatc aggctatcac
      121 gacgctgcgt gacgttgtct cgcggttgcc cctgggcaaa gactggcaga tacatgatga
      181 agccggggtt gccttcgacg ccgttgtcga tggccaattg caccttgccg gtaatcgccg
      241 cgaggccgga atcgcgcgcc gtctccatcg ccagccggcg caccgggttg gcccaggtat
      301 cgagacccag gggggcctgg tgacgtccgc tgtacgactc gcgctggatg accggagcgt
      361 aagtctcgcg ctccccttgc ggatgaatgc tgtagtcggc aggaccgagc cggcggatgg
      421 cgcggacatg cgccgctttc ccggccgacg gcacccgttc gatcaccccg atgacctgca
      481 tgcccgagag gttgggatca ccccggaggg tttcgacgta gtcgtgaatg gcttggcgat
      541 tccgcaggcc ggtggtggcg aacaggccct gcactccgcg cagcaattgc tcgtaggcgg
      601 cgacgcgctg ttcaatgcgg ctgacggtgt cgcgcagcgc gaagtcgaac tgcgtatgca
      661 gttccttgcg ggcattctgg cgttcgtgat cccagacgaa ccaggtcgcg ccgagcatgg
      721 cgaacaggat cagccagggc agaaaaagca gtcgcgatgc cggcggcgag acgcttgcgg
      781 tggtcatggc ggcgactaga agtcgaccat cgcggcggcc aggtcggcct tgaagtattt
      841 ttcgaaaatg cgacgcatgc tgccatcgct gcgcatgcca ttgatcaggg cacgccactt
      901 ctcctgctcg gccggcggca gcgacgtctt cgacatgacc agaccgtgcg gaaccgtcgc
      961 gtcgctgaac tcgatgatgc tcgtgacgtc gcgaatccgc ttttcttcga gtgtcgggta
     1021 gtcgaaaggc tcgatgatca tgccgtggat gtgacccagc agcagggtct ggtacaacgg
     1081 cgcgaggccc cccgcctggc tgacgcgact ggcggcgtgc aacttgtcca ccagccgatt
     1141 ggcggacggg ctgtaacgga agccgcgaat cacaccgagc tgcaacttgt cgttctgctc
     1201 gaaatcggcc tgattgcgca cgccggcatc cttgcgtacc aacaggtagt acttgttgct
     1261 gaagtaccag gcgaatccgg cgaatttgtc gcgctcggcg ttgctgatcc cggaaagggt
     1321 gaaatcgagg gcaccggatt caatcagttg ccagatccgg gcacgggcga gcagactggt
     1381 ggtgaccttg caaccgctgc ggcgaatcag ctcgtcggcg acgtccttgt cgatcccgct
     1441 gtccgtgtcg gcggaataga gcagcccatg ctcgtgcagt gccagcgtga atggccgcga
     1501 gcagtc
//
LOCUS       DAOSIT010000612         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_73186_length_1504_cov_7.639752, whole genome shotgun sequence.
ACCESSION   DAOSIT010000612 DAOSIT010000000
VERSION     DAOSIT010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73186_length_1504_cov_7.639752"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLL14_11520"
     CDS             complement(<1..1054)
                     /locus_tag="PLL14_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HOG04603.1"
                     /translation="MHKLLAIATLLLAVPASATVPRIVAPQEVRELLSDYLELGEVTD
                     APARAAFERRMQREVARLLATEGYFSPVVVLDQRGEELLLEVDPGARSRIGSVHIEIT
                     GPLDPERREALSRAWKLKAGQPFRQADWDEAKQSFLADLLAVDYAAAHLQETLAEVDP
                     EARRVDLRVVAVSGPRYRFGEVRISGLQRYSRDLVERFNRSVKPGEDYREERLLALQA
                     ALQSTPYFSSVNVSLERGEAADPPASVADERVTAPVRVLLRERAPYQLSLGAGYSTNT
                     GARVEAEYRSSDFFGRAWELQTGLRLEQVRQTAYADVFFPPDAGQGRDGVGTAVEHSD
                     IERLEIRRFAVGATRVQ"
BASE COUNT          281 a          491 c          472 g          260 t
ORIGIN      
        1 gctgcacacg tgtcgcaccg accgcaaagc gcctgatctc caggcgctcg atatcggagt
       61 gctcgaccgc ggtaccgacg ccgtcgcggc cttgccccgc gtccggcggg aaaaagacat
      121 cggcataggc ggtctgacgc acctgctcga ggcgcaggcc ggtctgcagc tcccaggcgc
      181 ggccgaagaa atcgctgctg cggtattctg cttcgacccg cgcgccggta ttggtgctgt
      241 aaccggcgcc cagcgacaac tgatacggtg cgcgctcgcg cagcagcacg cgcaccggcg
      301 cggtcacccg ctcatccgct acgcttgcag gcgggtcagc ggcctcaccc cgttccagag
      361 agacgttcac cgaagaaaag tacggcgtac tctgcaaggc ggcctgcaag gccagcaacc
      421 gctcttcgcg atagtcttcc cctggtttga cgctgcgatt gaagcgttcg acgagatccc
      481 gagaatagcg ctgcaggccg ctgatgcgca cttcgccgaa gcgatagcgc ggcccgctca
      541 cggcaacgac ccgcaggtcg acgcgccgtg cttcaggatc aacttcggca agggtctcct
      601 gaagatgggc ggcggcgtag tccaccgcca gcaggtcggc aagaaaggat tgcttggcct
      661 cgtcccaatc ggcttgccgg aagggctgcc cggctttgag tttccaggcc cttgacagcg
      721 cctcgcgacg ctccggatcg agcggaccgg tgatctcgat atgcacgctg ccgatccgcg
      781 aacgtgcccc gggatcgacc tccagcagca gttcctcgcc gcgctggtca agcacgacaa
      841 cgggcgagaa gtacccctcg gtagccagca acctggccac ctcacgctgc atccggcgct
      901 cgaaagccgc ccgcgccgga gcgtcggtga cctcgccgag ttccaggtaa tcactcagca
      961 gttcgcgcac ctcttgcggc gccacgatcc ggggcaccgt ggcggaagcc gggacggcga
     1021 gcagcagggt ggcaatggca aggagcttgt gcatgagttt atcggatgcc ggcgtggcaa
     1081 gtggcgaatc ctggccttga gtattctgtc aatgagcggg tcgcattgtc gactcgcgaa
     1141 tctccccgct acggcaagcg tgtctggaca cgagaaaagt gttcatggcg ttgatgatcg
     1201 ggcaaagcgg gaatggcgat gaacgtagac tcgtcccggt ctggtggcgc cagtcgttgg
     1261 cctgacggcg tcgcaactac gggcatagga taaacccttt gccgacactg gcggaaagat
     1321 cagcccggtc ggcagccctg cccgggttcg gccggctccc ggcaagctcc cgggtatttc
     1381 gcggccaagc attgcgcctt gtgatctgat ttcggtatga tcaggaagag cctttgcgca
     1441 cgcaatttcc cccgaacttt cccccgaacc actcctatgg agttccacca tggcaaaagc
     1501 caaa
//
LOCUS       DAOSIT010000613         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_73266_length_1503_cov_10.904696, whole genome shotgun
            sequence.
ACCESSION   DAOSIT010000613 DAOSIT010000000
VERSION     DAOSIT010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73266_length_1503_cov_10.904696"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="mltB"
                     /locus_tag="PLL14_11525"
     CDS             <1..907
                     /gene="mltB"
                     /locus_tag="PLL14_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HOG04604.1"
                     /translation="AFIVDMHEQHGFDIAHLTRQFATIRPNMVVLRAIRPAAVPEQQR
                     SWQRYRERFLTSTRVDGGRAFWQRHAVDLQRAAATYGVPPEVIVAIIGVETVYGQNMG
                     SFGVLEALASLAFQYPPRAEFFRSELEQFLLLARENGVSPLSIKGSYAGAIGIPQFMP
                     SSQRRFAVDFDGDDRIDLRNSSSDAIGSVARFLQQHGWQQGAPIAVPATVKGDPAALI
                     AAGIKPAINVQEMAAYGVHAVADAERAAALIDLVTPGVATEYWLGFDNFYVITRYNRS
                     SFYAMAVFQLAESLREAHARITTSR"
     gene            complement(904..>1503)
                     /locus_tag="PLL14_11530"
     CDS             complement(904..>1503)
                     /locus_tag="PLL14_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG04605.1"
                     /translation="AIEKFDPERGFRFSTYATWWIRQSIERAIMNQSRTIRLPVHVVK
                     EINLVLRAMRHLESDNGREIRIDQIAHLIDRSVEDVRRVMTLNERIASLDTPLQVDPS
                     RTMVDVIADEGALDVDELLQTNEIGDLLKKWVGQLADKQQQVLRRRYGLADGEACTLE
                     EIALDLNLTRERVRQIQIEALDHLRRIIKRGGVTRDNLF"
BASE COUNT          287 a          488 c          432 g          296 t
ORIGIN      
        1 ggccttcatc gtcgacatgc acgaacagca cggcttcgat attgcccacc tgactcgcca
       61 attcgccacc atccgaccaa acatggtcgt gctgcgagcc atccggccgg cggcggtgcc
      121 cgaacaacag cgctcatggc aacgctatcg cgagcgcttc ctgaccagta cacgcgtcga
      181 cggcggtcgc gccttttggc aaagacatgc ggtcgatctg cagcgcgccg cagcgaccta
      241 tggcgttccg cccgaagtca tcgtggccat catcggggtc gaaacggtgt acggccagaa
      301 tatgggcagc ttcggggtcc tcgaagccct cgcttcgctg gccttccagt acccgccgcg
      361 ggccgagttt ttccgcagcg aactggagca gtttctactc ctggcacgcg agaacggcgt
      421 cagcccactg tccatcaagg gctcctatgc cggcgccatc ggtatcccgc aattcatgcc
      481 cagcagccag cgccgctttg ccgtcgactt cgatggcgac gatcgtatcg acctgcgcaa
      541 cagcagtagc gacgctattg gcagcgtcgc ccggtttctg caacagcatg gttggcagca
      601 aggcgctccg atagccgtcc cggccaccgt caaaggagat ccggcggcac ttatcgccgc
      661 tggcatcaaa cccgcgatca acgtccagga aatggccgcc tacggtgtcc acgcagtagc
      721 tgacgcggaa cgagcagcgg cgctgatcga cctggtgacg cctggggtgg caaccgaata
      781 ctggctcgga ttcgacaact tctacgtgat tacccgctac aaccgctcga gcttctacgc
      841 gatggccgtc ttccagctcg cagaatctct cagggaagcc cacgcccgca tcacgaccag
      901 tcgttagaac agattgtcgc gagtcacgcc gccgcgcttg atgatgcggc gaagatggtc
      961 gagtgcttca atctggatct ggcgaacgcg ttcacgggtg agattcaggt cgagggcaat
     1021 ttcctctagg gtgcaggctt cgccgtcagc aagaccgtaa cgacgtcgca gaacctgctg
     1081 ctgtttgtcg gcaagctgcc cgacccactt tttcagcaag tctccgattt cgttggtctg
     1141 caacagctca tcgacatcaa gcgccccttc gtcggcgatg acgtccacca tcgttcggct
     1201 ggggtccacc tgtagaggcg tgtcgagcga tgcgatacgc tcgttgagcg tcatcacgcg
     1261 ccgcacatcc tctaccgaac ggtcgatcag atgcgcgatc tggtcgatcc gaatctcccg
     1321 cccgttgtcc gactccagat gtcgcatggc gcgcagcacg aggttgattt ccttgaccac
     1381 atgcaccggc aggcggatcg tgcgcgactg gttcatgatg gcgcgctcga tgctttgcct
     1441 gatccaccag gtcgcgtaag tcgagaaccg gaatccacgc tcgggatcga acttttcgat
     1501 ggc
//
LOCUS       DAOSIT010000614         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Accumulibacter sp. isolate AMR_MDS_1694
            NODE_73433_length_1501_cov_7.556017, whole genome shotgun sequence.
ACCESSION   DAOSIT010000614 DAOSIT010000000
VERSION     DAOSIT010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863223
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Accumulibacter sp. (wastewater metagenome)
  ORGANISM  Accumulibacter sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Candidatus
            Accumulibacter.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:28:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,307
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,280
            CDSs (with protein)               :: 2,280
            Genes (RNA)                       :: 8
            tRNAs                             :: 8
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 5 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Accumulibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73433_length_1501_cov_7.556017"
                     /isolate="AMR_MDS_1694"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053492"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="PLL14_11535"
     CDS             <1..>1501
                     /locus_tag="PLL14_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOG04606.1"
                     /translation="LRGMNSDNARKVPADAPTAFIKPRWAKLVLTDDGIDRRYYELCA
                     LSELKNALRSGDVWVQGSRQFKDFDEYLVPAEKFATLKLANELSLAVATYCDQYLHDR
                     LALLEQQLATVNRMAAANDLPDAIITESGLKITPLDAAVPETAQALIDQSAMLLPHVK
                     ITELLMEVDEWTGFTRHFTHLKTGDTAKDKTLLLTTILADGINLGLTKMAESCPGTTY
                     AKLSWLQAWHIRDETYSTALAELVNAQFRQSFAGNWGDGTTSSSDGQNFRTGSKAEST
                     GHINPKYGSSPGRTFYTHISDQYAPFSTKVVNVGVRDSTYVLDGLLYHESDLRIEEHY
                     TDTAGFTDHVFGLMHLLGFRFAPRIRDLGDTKLFIPKGDAAYDALKPMISSDRLNIKQ
                     IRAHWDEILRFATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWL
                     QSVELRRRVQAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLN"
BASE COUNT          329 a          458 c          445 g          269 t
ORIGIN      
        1 cttcgcggca tgaacagcga caacgcccgc aaggtgcccg ccgacgcgcc gaccgcattc
       61 atcaagccac gctgggcgaa actggtgctc acggacgatg gcattgaccg gcgctactac
      121 gagctgtgcg ccctgtcaga gctaaagaac gcgctgcgat caggcgatgt ctgggtgcag
      181 ggttcgcgcc agttcaagga ctttgatgag tacctggtgc cggccgagaa gttcgctact
      241 ctgaagctgg ccaacgaatt gtcgctggca gtggccacct attgcgatca gtatttgcac
      301 gaccgactgg cattgctgga acagcagctt gccaccgtca accgcatggc ggcggccaat
      361 gatctgccgg atgccatcat taccgagtcc ggcctgaaga tcacgccgct ggacgcggcg
      421 gtgccggaga ccgcacaagc cctaatcgac cagtcggcga tgctgctgcc acacgtcaag
      481 attaccgaac tgctgatgga agttgatgag tggacaggct tcacccggca tttcacgcat
      541 ctgaagaccg gcgacacggc caaggacaaa accttgctgc tgacgacgat cctggctgat
      601 ggcatcaatc tcgggctaac caagatggcc gagtcctgcc caggcacgac ttacgccaag
      661 ctgtcgtggc tgcaagcctg gcacatccgg gatgaaacgt actcaacggc gctggccgaa
      721 ctggtcaacg cgcagttccg gcaatccttt gccggaaact ggggcgatgg caccacgtca
      781 tcgtcggatg gccaaaattt cagaaccggc agcaaagcgg agagcaccgg gcatatcaac
      841 ccgaagtatg gaagcagtcc aggccggacg ttttataccc atatctccga ccagtacgcg
      901 cccttcagca ccaaggtagt caacgtcggc gtgcgtgatt caacctatgt gctcgatggc
      961 ctgctgtacc acgagtcgga cttgcggatc gaggagcact acaccgacac agcgggcttt
     1021 accgatcatg tcttcggcct gatgcaccta ctgggcttcc gattcgcgcc acgtatccga
     1081 gacctgggcg acaccaagct attcatccca aagggcgatg ccgcctatga cgcgctcaag
     1141 ccgatgatta gcagcgaccg gctgaacatc aagcagatac gcgcccattg ggatgaaatc
     1201 ttgcggttcg ccacttcgat caagcagggt acggtgacgg cctcgctgat gttgcgcaag
     1261 ctcggcagct atccgcgtca gaacggactg gccgttgcac tgcgcgagct ggggcgcatt
     1321 gagcgcacgc tgttcatact ggattggctg caaagcgtgg agctgcgccg ccgcgtgcag
     1381 gccggactca acaaaggcga ggcgcgcaac gcgctggcca gggcggtgtt cttcaaccga
     1441 ctgggcgaaa tccgcgaccg cagttttgag caacaacgct accgggccag cggcctcaat
     1501 c
//
